rapgef4 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	rapgef4
Entrez Gene ID	559170
Full Name	Rap guanine nucleotide exchange factor (GEF) 4
Synonyms	si:dkey-15i8.2
General protein information	Preferred Names Rap guanine nucleotide exchange factor (GEF) 4 Names rap guanine nucleotide exchange factor 4
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009304430.2	XP_009302705.1	rap guanine nucleotide exchange factor 4 isoform X1
XM_009304431.2	XP_009302706.1	rap guanine nucleotide exchange factor 4 isoform X2
XM_017357737.1	XP_017213226.1	rap guanine nucleotide exchange factor 4 isoform X3
XM_009304430.3	XP_009302705.1	rap guanine nucleotide exchange factor 4 isoform X2
XM_021478818.1	XP_021334493.1	rap guanine nucleotide exchange factor 4 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Rapgef4	XP_006234430.1
Xenopus tropicalis (tropical clawed frog)	rapgef4	XP_002934663.2
Pan troglodytes (chimpanzee)	RAPGEF4	XP_003309469.1
Canis lupus familiaris (dog)	RAPGEF4	XP_005640372.1
Mus musculus (house mouse)	Rapgef4	NP_001191094.1
Homo sapiens (human)	RAPGEF4	NP_008954.2
Macaca mulatta (Rhesus monkey)	RAPGEF4	XP_001086678.2
Bos taurus (cattle)	RAPGEF4	NP_001179831.1
Gallus gallus (chicken)	RAPGEF4	XP_426579.3
Danio rerio (zebrafish)	rapgef4	XP_687578.4
Drosophila melanogaster (fruit fly)	Epac	NP_001097202.2
Caenorhabditis elegans (roundworm)	epac-1	NP_499256.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007307	XP_308514.4

Gene Ontology

The following rapgef4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rapgef4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa60283	XM_017357737.1 Latest version!	Danio rerio Rap guanine nucleotide exchange factor (GEF) 4 (rapgef4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ODa42997	XM_009304430.3 Latest version!	Danio rerio Rap guanine nucleotide exchange factor (GEF) 4 (rapgef4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa42998	XM_009304431.2 Latest version!	Danio rerio Rap guanine nucleotide exchange factor (GEF) 4 (rapgef4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa144032	XM_021478818.1 Latest version!	Danio rerio Rap guanine nucleotide exchange factor (GEF) 4 (rapgef4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
ODa42997	XM_009304430.2	Danio rerio Rap guanine nucleotide exchange factor (GEF) 4 (rapgef4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rapgef4 ( XM_017357737.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213226.1 Danio rerio Rap guanine nucleotide exchange factor (GEF) 4 (rapgef4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa60283
Clone ID Related Accession (Same CDS sequence)	XM_017357737.1
Accession Version	XM_017357737.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1464bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	rap guanine nucleotide exchange factor 4 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGTT TGCTGGTCCC TCCCTATGGT GTTATGGACA GCGGCCCAAA CGCTGACAGA 
ATGCCTGACA AAGAGAATCT GAGCAACAGT TCTCCTGGAC TATCACCCAA GCACTTAAAT 
AAGAAATCAT TCATTGAAAA TCCCATCAAA GTTCATCCCA AACCCATTAA TCAGGTGCCG 
TCAGAGAAGA TACTTCGGGC AGGAAAAGTG CTACGAAACA TCATTTTATC TCGAGCGCCT 
CATATGATAA GAGACAGGAA ATACCACCTG AAGACGTACA GGCGGAGCTG CATCGGCACT 
GAGCTCGTTG ATTGGCTGTT GCAGCACACA TCATGTGTTC ACTCTCGTGT TCAGGCTGTA 
GGGATGTGGC AGGTGCTGCT GGAGGAGGGA GTGCTTAACC ACGTGGACCA GGAGCCGACT 
TTCCAAGATA AATATCTGTT TTATCGCTTT CTGGATGACG AAAGTGAGGA TTCAGGAATG 
CCGAGTGAGG AAGACAAGAA GGAGAGTGAC GAAGAGCTGC AGGACACGCT TCTGCTGCTG 
TCTCAGATCG GCCCTGATGC TCATCTGCGG ATGATCCTGC GCAAACAACC CGGCCAGAGG 
ACGGTCGATG ACCTGGAGAT CATTTATGAG GAGCTGCTGC ACATTAAAGC CTTGTCCCAT 
CTCTCCACCA CTGTGAAGAG GGAACTCGCT GGTGTTTTAA TATTTGAGTC GCATGCGAAA 
GCAGGCACAG TGTTGTTCAA TCAGGGTGAA GAAGGGACGT CCTGGTACAT CATCCTGAAG 
GGCTCCGTCA ATGTGGTCAT CTATGGAAAG GGTGTGGTGT GTACTCTACA TGAGGGTGAT 
GATTTCGGGA AGCTGGCTCT GGTGAATGAC GCTCCTCGGG CCGCCTCCAT CGTGCTGAGA 
GAAGACTTCT GCCACTTCCT CAGGGTGGAT AAAGAAGACT TCAACAGAAT CCTCAGGGAT 
GTGGAGGCAA ACACTGTCCG TCTGAAGGAA CATGAAGAGG ACGTGCTGGT TTTGGAGAAA 
GCTAACAACA GGAGCTCCAG TTCTTCTCCT CTCAAGTACA CTGTGATGTC AGGAACTCCA 
GAGAAGATTC TGGAGCACCT TCTGGAGACC ATGAAACTTG ATTGCCAATT CTCAGAGTCA 
GATCCTGCTC TGGATGACTT TCTGCTCATG CACTGTGTTT TTGTCCCGAA CAGTCAGCTG 
TGTCCACTGC TCATGACCCA TTATCACGCT CAGGCGTCCC AGGGCAGTGA ACAGGAGCGG 
ATGGATTACA CTCTCAATAA CAAGCGCAGG GTGGTGCGTC TGGTGTTACA GTGGGCCTCC 
ATCAACACGG ACCACCTGCA GGAGGAGGAC AGCTCCTTCA GCTTCATACA GGAGTTTTAT 
GAGATGGTGT GTGAAGACTC CAGGCTCACT CCAGCCCTGA AGGACCATCT GCCCGAACTG 
GAGAAGATCA TCAAGCAGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213226.1
CDS	164..1627
Translation	MAGLLVPPYGVMDSGPNADRMPDKENLSNSSPGLSPKHLNKKSFIENPIKVHPKPINQVPSEKILRAGKVLRNIILSRAPHMIRDRKYHLKTYRRSCIGTELVDWLLQHTSCVHSRVQAVGMWQVLLEEGVLNHVDQEPTFQDKYLFYRFLDDESEDSGMPSEEDKKESDEELQDTLLLLSQIGPDAHLRMILRKQPGQRTVDDLEIIYEELLHIKALSHLSTTVKRELAGVLIFESHAKAGTVLFNQGEEGTSWYIILKGSVNVVIYGKGVVCTLHEGDDFGKLALVNDAPRAASIVLREDFCHFLRVDKEDFNRILRDVEANTVRLKEHEEDVLVLEKANNRSSSSSPLKYTVMSGTPEKILEHLLETMKLDCQFSESDPALDDFLLMHCVFVPNSQLCPLLMTHYHAQASQGSEQERMDYTLNNKRRVVRLVLQWASINTDHLQEEDSSFSFIQEFYEMVCEDSRLTPALKDHLPELEKIIKQK

Target ORF information:

RefSeq Version	XM_017357737.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Rap guanine nucleotide exchange factor (GEF) 4 (rapgef4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357737.1

ORF Insert Sequence:

ATGGCTGGTT TGCTGGTCCC TCCCTATGGT GTTATGGACA GCGGCCCAAA CGCTGACAGA 
ATGCCTGACA AAGAGAATCT GAGCAACAGT TCTCCTGGAC TATCACCCAA GCACTTAAAT 
AAGAAATCAT TCATTGAAAA TCCCATCAAA GTTCATCCCA AACCCATTAA TCAGGTGCCG 
TCAGAGAAGA TACTTCGGGC AGGAAAAGTG CTACGAAACA TCATTTTATC TCGAGCGCCT 
CATATGATAA GAGACAGGAA ATACCACCTG AAGACGTACA GGCGGAGCTG CATCGGCACT 
GAGCTCGTTG ATTGGCTGTT GCAGCACACA TCATGTGTTC ACTCTCGTGT TCAGGCTGTA 
GGGATGTGGC AGGTGCTGCT GGAGGAGGGA GTGCTTAACC ACGTGGACCA GGAGCCGACT 
TTCCAAGATA AATATCTGTT TTATCGCTTT CTGGATGACG AAAGTGAGGA TTCAGGAATG 
CCGAGTGAGG AAGACAAGAA GGAGAGTGAC GAAGAGCTGC AGGACACGCT TCTGCTGCTG 
TCTCAGATCG GCCCTGATGC TCATCTGCGG ATGATCCTGC GCAAACAACC CGGCCAGAGG 
ACGGTCGATG ACCTGGAGAT CATTTATGAG GAGCTGCTGC ACATTAAAGC CTTGTCCCAT 
CTCTCCACCA CTGTGAAGAG GGAACTCGCT GGTGTTTTAA TATTTGAGTC GCATGCGAAA 
GCAGGCACAG TGTTGTTCAA TCAGGGTGAA GAAGGGACGT CCTGGTACAT CATCCTGAAG 
GGCTCCGTCA ATGTGGTCAT CTATGGAAAG GGTGTGGTGT GTACTCTACA TGAGGGTGAT 
GATTTCGGGA AGCTGGCTCT GGTGAATGAC GCTCCTCGGG CCGCCTCCAT CGTGCTGAGA 
GAAGACTTCT GCCACTTCCT CAGGGTGGAT AAAGAAGACT TCAACAGAAT CCTCAGGGAT 
GTGGAGGCAA ACACTGTCCG TCTGAAGGAA CATGAAGAGG ACGTGCTGGT TTTGGAGAAA 
GCTAACAACA GGAGCTCCAG TTCTTCTCCT CTCAAGTACA CTGTGATGTC AGGAACTCCA 
GAGAAGATTC TGGAGCACCT TCTGGAGACC ATGAAACTTG ATTGCCAATT CTCAGAGTCA 
GATCCTGCTC TGGATGACTT TCTGCTCATG CACTGTGTTT TTGTCCCGAA CAGTCAGCTG 
TGTCCACTGC TCATGACCCA TTATCACGCT CAGGCGTCCC AGGGCAGTGA ACAGGAGCGG 
ATGGATTACA CTCTCAATAA CAAGCGCAGG GTGGTGCGTC TGGTGTTACA GTGGGCCTCC 
ATCAACACGG ACCACCTGCA GGAGGAGGAC AGCTCCTTCA GCTTCATACA GGAGTTTTAT 
GAGATGGTGT GTGAAGACTC CAGGCTCACT CCAGCCCTGA AGGACCATCT GCCCGAACTG 
GAGAAGATCA TCAAGCAGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rapgef4 ( XM_009304430.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302705.1 Danio rerio Rap guanine nucleotide exchange factor (GEF) 4 (rapgef4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa42997
Clone ID Related Accession (Same CDS sequence)	XM_009304430.2 , XM_009304430.3
Accession Version	XM_009304430.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	rap guanine nucleotide exchange factor 4 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304430.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGTT TGCTGGTCCC TCCCTATGGT GTTATGGACA GCGGCCCAAA CGCTGACAGA 
ATGCCTGACA AAGAGAATCT GAGCAACAGT TCTCCTGGAC TATCACCCAA GCACTTAAAT 
AAGAAATCAT TCATTGAAAA TCCCATCAAA GTTCATCCCA AACCCATTAA TCAGGTGCCG 
TCAGAGAAGA TACTTCGGGC AGGAAAAGTG CTACGAAACA TCATTTTATC TCGAGCGCCT 
CATATGATAA GAGACAGGAA ATACCACCTG AAGACGTACA GGCGGAGCTG CATCGGCACT 
GAGCTCGTTG ATTGGCTGTT GCAGCACACA TCATGTGTTC ACTCTCGTGT TCAGGCTGTA 
GGGATGTGGC AGGTGCTGCT GGAGGAGGGA GTGCTTAACC ACGTGGACCA GGAGCCGACT 
TTCCAAGATA AATATCTGTT TTATCGCTTT CTGGATGACG AAAGTGAGGA TTCAGGAATG 
CCGAGTGAGG AAGACAAGAA GGAGAGTGAC GAAGAGCTGC AGGACACGCT TCTGCTGCTG 
TCTCAGATCG GCCCTGATGC TCATCTGCGG ATGATCCTGC GCAAACAACC CGGCCAGAGG 
ACGGTCGATG ACCTGGAGAT CATTTATGAG GAGCTGCTGC ACATTAAAGC CTTGTCCCAT 
CTCTCCACCA CTGTGAAGAG GGAACTCGCT GGTGTTTTAA TATTTGAGTC GCATGCGAAA 
GCAGGCACAG TGTTGTTCAA TCAGGGTGAA GAAGGGACGT CCTGGTACAT CATCCTGAAG 
GGCTCCGTCA ATGTGGTCAT CTATGGAAAG GGTGTGGTGT GTACTCTACA TGAGGGTGAT 
GATTTCGGGA AGCTGGCTCT GGTGAATGAC GCTCCTCGGG CCGCCTCCAT CGTGCTGAGA 
GAAGACTTCT GCCACTTCCT CAGGGTGGAT AAAGAAGACT TCAACAGAAT CCTCAGGGAT 
GTGGAGGCAA ACACTGTCCG TCTGAAGGAA CATGAAGAGG ACGTGCTGGT TTTGGAGAAA 
GCTAACAACA GGAGCTCCAG TTCTTCTCCT CTCAAGTACA CTGTGATGTC AGGAACTCCA 
GAGAAGATTC TGGAGCACCT TCTGGAGACC ATGAAACTTG ATTGCCAATT CTCAGAGTCA 
GATCCTGCTC TGGATGACTT TCTGCTCATG CACTGTGTTT TTGTCCCGAA CAGTCAGCTG 
TGTCCACTGC TCATGACCCA TTATCACGCT CAGGCGTCCC AGGGCAGTGA ACAGGAGCGG 
ATGGATTACA CTCTCAATAA CAAGCGCAGG GTGGTGCGTC TGGTGTTACA GTGGGCCTCC 
ATCAACACGG ACCACCTGCA GGAGGAGGAC AGCTCCTTCA GCTTCATACA GGAGTTTTAT 
GAGATGGTGT GTGAAGACTC CAGGCTCACT CCAGCCCTGA AGGACCATCT GCCCGAACTG 
GAGAAGATCA TCAAGCAGAA CTCAGATGAC GGCAGACCCT CTCAGAAAAA GCACAAAGTC 
CTGCTGCGTC AGTTCAGCAC AGGGAACGAC AGGCTTCAGA AGAGACAGCC CATCAAAAGC 
ACAGACGAGA TCCTCTTCAA GGTGTACTGC AGTGATCACA CCTACTCTAC TATCCGTGTG 
CCCGTGTCTG CATCCGTCAG AGAGGTCATC GCTGCTGTAT CCGACAAACT GAGCTCAGGA 
GACGATCTGC TGCTCATCCA TCTCAACTCA GCTGGAGATA AAGAGCTTCT GAAGCCGTCT 
GATGTTTCCA TCTTCTCATC TCTCAGCATT AATGGCCGTC TGTTCGTCTG CCCGAGGGAC 
CAAATCGACT CTTTGACGCC GCTGCCCGAG CAGGAGGGAC CGTCTGCAGG CTCCATGTCC 
ACGTTTGAGC TCATGAGCTC TAAAGATCTG GCCCATCAGA TGACCCTCTA CGACTGGGAG 
CTCTTCCACT GTGTGCATGA GCATGAGCTG ATCTACCACA CGTTCGGCCG ACATCATTTT 
AAGAAGACCA CCGCCAATCT GGACCTGTTC CTGCGGCGCT TCAATGAGGT GCAGCTGTGG 
GTGGTGACGG AGGTCTGTCT GTGTCCCGCG CTCAGCAAAC GAGTGCAGCT CCTCAAGAAG 
TTCATCAAGA TCGCTGCACA CTGTAAGGAG TTTAAGAACC TGAACTCATT CTTTGCCATT 
ATCATGGGTC TGGGAAACCC TGCGGTCTGT AGACTCAGTC AGACATGGGA GAAGCTGCCC 
AGCAAATTCA AGAAGTTTTA TGGAGAGTTT GAAAACCTAA TGGATCCCTC CAGAAATCAC 
AGAGCATATC GACTTACAAT GGCCAAGCTA GAACCCCCCA TCATCCCCTT CATGCCCTTA 
TTAATTAAAG ACATGACTTT CACACACGAG GGAAACAAGA CCTTCACTGA CCGACTGGTC 
AACTTTGAGA AGATGAGAAT GATTGCAAAC ACAGTCAGAA TCATAAGATA CTGCAGAAGT 
CAACCATTTG ATCAGGAAGC TCCGCAGGCG ACGGGTAAAA GCCATCAGGA CGTGCGCACT 
TACGTCCGCC ACATCAGCGT GATCGATAAC CAGCGCAGTC TATCACAGCT GTCCCACCGT 
CTAGAGCCCC GGCGGACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302705.1
CDS	163..2763
Translation	MAGLLVPPYGVMDSGPNADRMPDKENLSNSSPGLSPKHLNKKSFIENPIKVHPKPINQVPSEKILRAGKVLRNIILSRAPHMIRDRKYHLKTYRRSCIGTELVDWLLQHTSCVHSRVQAVGMWQVLLEEGVLNHVDQEPTFQDKYLFYRFLDDESEDSGMPSEEDKKESDEELQDTLLLLSQIGPDAHLRMILRKQPGQRTVDDLEIIYEELLHIKALSHLSTTVKRELAGVLIFESHAKAGTVLFNQGEEGTSWYIILKGSVNVVIYGKGVVCTLHEGDDFGKLALVNDAPRAASIVLREDFCHFLRVDKEDFNRILRDVEANTVRLKEHEEDVLVLEKANNRSSSSSPLKYTVMSGTPEKILEHLLETMKLDCQFSESDPALDDFLLMHCVFVPNSQLCPLLMTHYHAQASQGSEQERMDYTLNNKRRVVRLVLQWASINTDHLQEEDSSFSFIQEFYEMVCEDSRLTPALKDHLPELEKIIKQNSDDGRPSQKKHKVLLRQFSTGNDRLQKRQPIKSTDEILFKVYCSDHTYSTIRVPVSASVREVIAAVSDKLSSGDDLLLIHLNSAGDKELLKPSDVSIFSSLSINGRLFVCPRDQIDSLTPLPEQEGPSAGSMSTFELMSSKDLAHQMTLYDWELFHCVHEHELIYHTFGRHHFKKTTANLDLFLRRFNEVQLWVVTEVCLCPALSKRVQLLKKFIKIAAHCKEFKNLNSFFAIIMGLGNPAVCRLSQTWEKLPSKFKKFYGEFENLMDPSRNHRAYRLTMAKLEPPIIPFMPLLIKDMTFTHEGNKTFTDRLVNFEKMRMIANTVRIIRYCRSQPFDQEAPQATGKSHQDVRTYVRHISVIDNQRSLSQLSHRLEPRRT

Target ORF information:

RefSeq Version	XM_009304430.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Rap guanine nucleotide exchange factor (GEF) 4 (rapgef4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304430.2

ORF Insert Sequence:

ATGGCTGGTT TGCTGGTCCC TCCCTATGGT GTTATGGACA GCGGCCCAAA CGCTGACAGA 
ATGCCTGACA AAGAGAATCT GAGCAACAGT TCTCCTGGAC TATCACCCAA GCACTTAAAT 
AAGAAATCAT TCATTGAAAA TCCCATCAAA GTTCATCCCA AACCCATTAA TCAGGTGCCG 
TCAGAGAAGA TACTTCGGGC AGGAAAAGTG CTACGAAACA TCATTTTATC TCGAGCGCCT 
CATATGATAA GAGACAGGAA ATACCACCTG AAGACGTACA GGCGGAGCTG CATCGGCACT 
GAGCTCGTTG ATTGGCTGTT GCAGCACACA TCATGTGTTC ACTCTCGTGT TCAGGCTGTA 
GGGATGTGGC AGGTGCTGCT GGAGGAGGGA GTGCTTAACC ACGTGGACCA GGAGCCGACT 
TTCCAAGATA AATATCTGTT TTATCGCTTT CTGGATGACG AAAGTGAGGA TTCAGGAATG 
CCGAGTGAGG AAGACAAGAA GGAGAGTGAC GAAGAGCTGC AGGACACGCT TCTGCTGCTG 
TCTCAGATCG GCCCTGATGC TCATCTGCGG ATGATCCTGC GCAAACAACC CGGCCAGAGG 
ACGGTCGATG ACCTGGAGAT CATTTATGAG GAGCTGCTGC ACATTAAAGC CTTGTCCCAT 
CTCTCCACCA CTGTGAAGAG GGAACTCGCT GGTGTTTTAA TATTTGAGTC GCATGCGAAA 
GCAGGCACAG TGTTGTTCAA TCAGGGTGAA GAAGGGACGT CCTGGTACAT CATCCTGAAG 
GGCTCCGTCA ATGTGGTCAT CTATGGAAAG GGTGTGGTGT GTACTCTACA TGAGGGTGAT 
GATTTCGGGA AGCTGGCTCT GGTGAATGAC GCTCCTCGGG CCGCCTCCAT CGTGCTGAGA 
GAAGACTTCT GCCACTTCCT CAGGGTGGAT AAAGAAGACT TCAACAGAAT CCTCAGGGAT 
GTGGAGGCAA ACACTGTCCG TCTGAAGGAA CATGAAGAGG ACGTGCTGGT TTTGGAGAAA 
GCTAACAACA GGAGCTCCAG TTCTTCTCCT CTCAAGTACA CTGTGATGTC AGGAACTCCA 
GAGAAGATTC TGGAGCACCT TCTGGAGACC ATGAAACTTG ATTGCCAATT CTCAGAGTCA 
GATCCTGCTC TGGATGACTT TCTGCTCATG CACTGTGTTT TTGTCCCGAA CAGTCAGCTG 
TGTCCACTGC TCATGACCCA TTATCACGCT CAGGCGTCCC AGGGCAGTGA ACAGGAGCGG 
ATGGATTACA CTCTCAATAA CAAGCGCAGG GTGGTGCGTC TGGTGTTACA GTGGGCCTCC 
ATCAACACGG ACCACCTGCA GGAGGAGGAC AGCTCCTTCA GCTTCATACA GGAGTTTTAT 
GAGATGGTGT GTGAAGACTC CAGGCTCACT CCAGCCCTGA AGGACCATCT GCCCGAACTG 
GAGAAGATCA TCAAGCAGAA CTCAGATGAC GGCAGACCCT CTCAGAAAAA GCACAAAGTC 
CTGCTGCGTC AGTTCAGCAC AGGGAACGAC AGGCTTCAGA AGAGACAGCC CATCAAAAGC 
ACAGACGAGA TCCTCTTCAA GGTGTACTGC AGTGATCACA CCTACTCTAC TATCCGTGTG 
CCCGTGTCTG CATCCGTCAG AGAGGTCATC GCTGCTGTAT CCGACAAACT GAGCTCAGGA 
GACGATCTGC TGCTCATCCA TCTCAACTCA GCTGGAGATA AAGAGCTTCT GAAGCCGTCT 
GATGTTTCCA TCTTCTCATC TCTCAGCATT AATGGCCGTC TGTTCGTCTG CCCGAGGGAC 
CAAATCGACT CTTTGACGCC GCTGCCCGAG CAGGAGGGAC CGTCTGCAGG CTCCATGTCC 
ACGTTTGAGC TCATGAGCTC TAAAGATCTG GCCCATCAGA TGACCCTCTA CGACTGGGAG 
CTCTTCCACT GTGTGCATGA GCATGAGCTG ATCTACCACA CGTTCGGCCG ACATCATTTT 
AAGAAGACCA CCGCCAATCT GGACCTGTTC CTGCGGCGCT TCAATGAGGT GCAGCTGTGG 
GTGGTGACGG AGGTCTGTCT GTGTCCCGCG CTCAGCAAAC GAGTGCAGCT CCTCAAGAAG 
TTCATCAAGA TCGCTGCACA CTGTAAGGAG TTTAAGAACC TGAACTCATT CTTTGCCATT 
ATCATGGGTC TGGGAAACCC TGCGGTCTGT AGACTCAGTC AGACATGGGA GAAGCTGCCC 
AGCAAATTCA AGAAGTTTTA TGGAGAGTTT GAAAACCTAA TGGATCCCTC CAGAAATCAC 
AGAGCATATC GACTTACAAT GGCCAAGCTA GAACCCCCCA TCATCCCCTT CATGCCCTTA 
TTAATTAAAG ACATGACTTT CACACACGAG GGAAACAAGA CCTTCACTGA CCGACTGGTC 
AACTTTGAGA AGATGAGAAT GATTGCAAAC ACAGTCAGAA TCATAAGATA CTGCAGAAGT 
CAACCATTTG ATCAGGAAGC TCCGCAGGCG ACGGGTAAAA GCCATCAGGA CGTGCGCACT 
TACGTCCGCC ACATCAGCGT GATCGATAAC CAGCGCAGTC TATCACAGCT GTCCCACCGT 
CTAGAGCCCC GGCGGACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rapgef4 ( XM_009304430.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302705.1 Danio rerio Rap guanine nucleotide exchange factor (GEF) 4 (rapgef4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa42997
Clone ID Related Accession (Same CDS sequence)	XM_009304430.2 , XM_009304430.3
Accession Version	XM_009304430.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	rap guanine nucleotide exchange factor 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304430.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGTT TGCTGGTCCC TCCCTATGGT GTTATGGACA GCGGCCCAAA CGCTGACAGA 
ATGCCTGACA AAGAGAATCT GAGCAACAGT TCTCCTGGAC TATCACCCAA GCACTTAAAT 
AAGAAATCAT TCATTGAAAA TCCCATCAAA GTTCATCCCA AACCCATTAA TCAGGTGCCG 
TCAGAGAAGA TACTTCGGGC AGGAAAAGTG CTACGAAACA TCATTTTATC TCGAGCGCCT 
CATATGATAA GAGACAGGAA ATACCACCTG AAGACGTACA GGCGGAGCTG CATCGGCACT 
GAGCTCGTTG ATTGGCTGTT GCAGCACACA TCATGTGTTC ACTCTCGTGT TCAGGCTGTA 
GGGATGTGGC AGGTGCTGCT GGAGGAGGGA GTGCTTAACC ACGTGGACCA GGAGCCGACT 
TTCCAAGATA AATATCTGTT TTATCGCTTT CTGGATGACG AAAGTGAGGA TTCAGGAATG 
CCGAGTGAGG AAGACAAGAA GGAGAGTGAC GAAGAGCTGC AGGACACGCT TCTGCTGCTG 
TCTCAGATCG GCCCTGATGC TCATCTGCGG ATGATCCTGC GCAAACAACC CGGCCAGAGG 
ACGGTCGATG ACCTGGAGAT CATTTATGAG GAGCTGCTGC ACATTAAAGC CTTGTCCCAT 
CTCTCCACCA CTGTGAAGAG GGAACTCGCT GGTGTTTTAA TATTTGAGTC GCATGCGAAA 
GCAGGCACAG TGTTGTTCAA TCAGGGTGAA GAAGGGACGT CCTGGTACAT CATCCTGAAG 
GGCTCCGTCA ATGTGGTCAT CTATGGAAAG GGTGTGGTGT GTACTCTACA TGAGGGTGAT 
GATTTCGGGA AGCTGGCTCT GGTGAATGAC GCTCCTCGGG CCGCCTCCAT CGTGCTGAGA 
GAAGACTTCT GCCACTTCCT CAGGGTGGAT AAAGAAGACT TCAACAGAAT CCTCAGGGAT 
GTGGAGGCAA ACACTGTCCG TCTGAAGGAA CATGAAGAGG ACGTGCTGGT TTTGGAGAAA 
GCTAACAACA GGAGCTCCAG TTCTTCTCCT CTCAAGTACA CTGTGATGTC AGGAACTCCA 
GAGAAGATTC TGGAGCACCT TCTGGAGACC ATGAAACTTG ATTGCCAATT CTCAGAGTCA 
GATCCTGCTC TGGATGACTT TCTGCTCATG CACTGTGTTT TTGTCCCGAA CAGTCAGCTG 
TGTCCACTGC TCATGACCCA TTATCACGCT CAGGCGTCCC AGGGCAGTGA ACAGGAGCGG 
ATGGATTACA CTCTCAATAA CAAGCGCAGG GTGGTGCGTC TGGTGTTACA GTGGGCCTCC 
ATCAACACGG ACCACCTGCA GGAGGAGGAC AGCTCCTTCA GCTTCATACA GGAGTTTTAT 
GAGATGGTGT GTGAAGACTC CAGGCTCACT CCAGCCCTGA AGGACCATCT GCCCGAACTG 
GAGAAGATCA TCAAGCAGAA CTCAGATGAC GGCAGACCCT CTCAGAAAAA GCACAAAGTC 
CTGCTGCGTC AGTTCAGCAC AGGGAACGAC AGGCTTCAGA AGAGACAGCC CATCAAAAGC 
ACAGACGAGA TCCTCTTCAA GGTGTACTGC AGTGATCACA CCTACTCTAC TATCCGTGTG 
CCCGTGTCTG CATCCGTCAG AGAGGTCATC GCTGCTGTAT CCGACAAACT GAGCTCAGGA 
GACGATCTGC TGCTCATCCA TCTCAACTCA GCTGGAGATA AAGAGCTTCT GAAGCCGTCT 
GATGTTTCCA TCTTCTCATC TCTCAGCATT AATGGCCGTC TGTTCGTCTG CCCGAGGGAC 
CAAATCGACT CTTTGACGCC GCTGCCCGAG CAGGAGGGAC CGTCTGCAGG CTCCATGTCC 
ACGTTTGAGC TCATGAGCTC TAAAGATCTG GCCCATCAGA TGACCCTCTA CGACTGGGAG 
CTCTTCCACT GTGTGCATGA GCATGAGCTG ATCTACCACA CGTTCGGCCG ACATCATTTT 
AAGAAGACCA CCGCCAATCT GGACCTGTTC CTGCGGCGCT TCAATGAGGT GCAGCTGTGG 
GTGGTGACGG AGGTCTGTCT GTGTCCCGCG CTCAGCAAAC GAGTGCAGCT CCTCAAGAAG 
TTCATCAAGA TCGCTGCACA CTGTAAGGAG TTTAAGAACC TGAACTCATT CTTTGCCATT 
ATCATGGGTC TGGGAAACCC TGCGGTCTGT AGACTCAGTC AGACATGGGA GAAGCTGCCC 
AGCAAATTCA AGAAGTTTTA TGGAGAGTTT GAAAACCTAA TGGATCCCTC CAGAAATCAC 
AGAGCATATC GACTTACAAT GGCCAAGCTA GAACCCCCCA TCATCCCCTT CATGCCCTTA 
TTAATTAAAG ACATGACTTT CACACACGAG GGAAACAAGA CCTTCACTGA CCGACTGGTC 
AACTTTGAGA AGATGAGAAT GATTGCAAAC ACAGTCAGAA TCATAAGATA CTGCAGAAGT 
CAACCATTTG ATCAGGAAGC TCCGCAGGCG ACGGGTAAAA GCCATCAGGA CGTGCGCACT 
TACGTCCGCC ACATCAGCGT GATCGATAAC CAGCGCAGTC TATCACAGCT GTCCCACCGT 
CTAGAGCCCC GGCGGACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302705.1
CDS	182..2782
Translation	MAGLLVPPYGVMDSGPNADRMPDKENLSNSSPGLSPKHLNKKSFIENPIKVHPKPINQVPSEKILRAGKVLRNIILSRAPHMIRDRKYHLKTYRRSCIGTELVDWLLQHTSCVHSRVQAVGMWQVLLEEGVLNHVDQEPTFQDKYLFYRFLDDESEDSGMPSEEDKKESDEELQDTLLLLSQIGPDAHLRMILRKQPGQRTVDDLEIIYEELLHIKALSHLSTTVKRELAGVLIFESHAKAGTVLFNQGEEGTSWYIILKGSVNVVIYGKGVVCTLHEGDDFGKLALVNDAPRAASIVLREDFCHFLRVDKEDFNRILRDVEANTVRLKEHEEDVLVLEKANNRSSSSSPLKYTVMSGTPEKILEHLLETMKLDCQFSESDPALDDFLLMHCVFVPNSQLCPLLMTHYHAQASQGSEQERMDYTLNNKRRVVRLVLQWASINTDHLQEEDSSFSFIQEFYEMVCEDSRLTPALKDHLPELEKIIKQNSDDGRPSQKKHKVLLRQFSTGNDRLQKRQPIKSTDEILFKVYCSDHTYSTIRVPVSASVREVIAAVSDKLSSGDDLLLIHLNSAGDKELLKPSDVSIFSSLSINGRLFVCPRDQIDSLTPLPEQEGPSAGSMSTFELMSSKDLAHQMTLYDWELFHCVHEHELIYHTFGRHHFKKTTANLDLFLRRFNEVQLWVVTEVCLCPALSKRVQLLKKFIKIAAHCKEFKNLNSFFAIIMGLGNPAVCRLSQTWEKLPSKFKKFYGEFENLMDPSRNHRAYRLTMAKLEPPIIPFMPLLIKDMTFTHEGNKTFTDRLVNFEKMRMIANTVRIIRYCRSQPFDQEAPQATGKSHQDVRTYVRHISVIDNQRSLSQLSHRLEPRRT

Target ORF information:

RefSeq Version	XM_009304430.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rap guanine nucleotide exchange factor (GEF) 4 (rapgef4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304430.3

ORF Insert Sequence:

ATGGCTGGTT TGCTGGTCCC TCCCTATGGT GTTATGGACA GCGGCCCAAA CGCTGACAGA 
ATGCCTGACA AAGAGAATCT GAGCAACAGT TCTCCTGGAC TATCACCCAA GCACTTAAAT 
AAGAAATCAT TCATTGAAAA TCCCATCAAA GTTCATCCCA AACCCATTAA TCAGGTGCCG 
TCAGAGAAGA TACTTCGGGC AGGAAAAGTG CTACGAAACA TCATTTTATC TCGAGCGCCT 
CATATGATAA GAGACAGGAA ATACCACCTG AAGACGTACA GGCGGAGCTG CATCGGCACT 
GAGCTCGTTG ATTGGCTGTT GCAGCACACA TCATGTGTTC ACTCTCGTGT TCAGGCTGTA 
GGGATGTGGC AGGTGCTGCT GGAGGAGGGA GTGCTTAACC ACGTGGACCA GGAGCCGACT 
TTCCAAGATA AATATCTGTT TTATCGCTTT CTGGATGACG AAAGTGAGGA TTCAGGAATG 
CCGAGTGAGG AAGACAAGAA GGAGAGTGAC GAAGAGCTGC AGGACACGCT TCTGCTGCTG 
TCTCAGATCG GCCCTGATGC TCATCTGCGG ATGATCCTGC GCAAACAACC CGGCCAGAGG 
ACGGTCGATG ACCTGGAGAT CATTTATGAG GAGCTGCTGC ACATTAAAGC CTTGTCCCAT 
CTCTCCACCA CTGTGAAGAG GGAACTCGCT GGTGTTTTAA TATTTGAGTC GCATGCGAAA 
GCAGGCACAG TGTTGTTCAA TCAGGGTGAA GAAGGGACGT CCTGGTACAT CATCCTGAAG 
GGCTCCGTCA ATGTGGTCAT CTATGGAAAG GGTGTGGTGT GTACTCTACA TGAGGGTGAT 
GATTTCGGGA AGCTGGCTCT GGTGAATGAC GCTCCTCGGG CCGCCTCCAT CGTGCTGAGA 
GAAGACTTCT GCCACTTCCT CAGGGTGGAT AAAGAAGACT TCAACAGAAT CCTCAGGGAT 
GTGGAGGCAA ACACTGTCCG TCTGAAGGAA CATGAAGAGG ACGTGCTGGT TTTGGAGAAA 
GCTAACAACA GGAGCTCCAG TTCTTCTCCT CTCAAGTACA CTGTGATGTC AGGAACTCCA 
GAGAAGATTC TGGAGCACCT TCTGGAGACC ATGAAACTTG ATTGCCAATT CTCAGAGTCA 
GATCCTGCTC TGGATGACTT TCTGCTCATG CACTGTGTTT TTGTCCCGAA CAGTCAGCTG 
TGTCCACTGC TCATGACCCA TTATCACGCT CAGGCGTCCC AGGGCAGTGA ACAGGAGCGG 
ATGGATTACA CTCTCAATAA CAAGCGCAGG GTGGTGCGTC TGGTGTTACA GTGGGCCTCC 
ATCAACACGG ACCACCTGCA GGAGGAGGAC AGCTCCTTCA GCTTCATACA GGAGTTTTAT 
GAGATGGTGT GTGAAGACTC CAGGCTCACT CCAGCCCTGA AGGACCATCT GCCCGAACTG 
GAGAAGATCA TCAAGCAGAA CTCAGATGAC GGCAGACCCT CTCAGAAAAA GCACAAAGTC 
CTGCTGCGTC AGTTCAGCAC AGGGAACGAC AGGCTTCAGA AGAGACAGCC CATCAAAAGC 
ACAGACGAGA TCCTCTTCAA GGTGTACTGC AGTGATCACA CCTACTCTAC TATCCGTGTG 
CCCGTGTCTG CATCCGTCAG AGAGGTCATC GCTGCTGTAT CCGACAAACT GAGCTCAGGA 
GACGATCTGC TGCTCATCCA TCTCAACTCA GCTGGAGATA AAGAGCTTCT GAAGCCGTCT 
GATGTTTCCA TCTTCTCATC TCTCAGCATT AATGGCCGTC TGTTCGTCTG CCCGAGGGAC 
CAAATCGACT CTTTGACGCC GCTGCCCGAG CAGGAGGGAC CGTCTGCAGG CTCCATGTCC 
ACGTTTGAGC TCATGAGCTC TAAAGATCTG GCCCATCAGA TGACCCTCTA CGACTGGGAG 
CTCTTCCACT GTGTGCATGA GCATGAGCTG ATCTACCACA CGTTCGGCCG ACATCATTTT 
AAGAAGACCA CCGCCAATCT GGACCTGTTC CTGCGGCGCT TCAATGAGGT GCAGCTGTGG 
GTGGTGACGG AGGTCTGTCT GTGTCCCGCG CTCAGCAAAC GAGTGCAGCT CCTCAAGAAG 
TTCATCAAGA TCGCTGCACA CTGTAAGGAG TTTAAGAACC TGAACTCATT CTTTGCCATT 
ATCATGGGTC TGGGAAACCC TGCGGTCTGT AGACTCAGTC AGACATGGGA GAAGCTGCCC 
AGCAAATTCA AGAAGTTTTA TGGAGAGTTT GAAAACCTAA TGGATCCCTC CAGAAATCAC 
AGAGCATATC GACTTACAAT GGCCAAGCTA GAACCCCCCA TCATCCCCTT CATGCCCTTA 
TTAATTAAAG ACATGACTTT CACACACGAG GGAAACAAGA CCTTCACTGA CCGACTGGTC 
AACTTTGAGA AGATGAGAAT GATTGCAAAC ACAGTCAGAA TCATAAGATA CTGCAGAAGT 
CAACCATTTG ATCAGGAAGC TCCGCAGGCG ACGGGTAAAA GCCATCAGGA CGTGCGCACT 
TACGTCCGCC ACATCAGCGT GATCGATAAC CAGCGCAGTC TATCACAGCT GTCCCACCGT 
CTAGAGCCCC GGCGGACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rapgef4 ( XM_009304431.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302706.1 Danio rerio Rap guanine nucleotide exchange factor (GEF) 4 (rapgef4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa42998
Clone ID Related Accession (Same CDS sequence)	XM_009304431.2
Accession Version	XM_009304431.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	rap guanine nucleotide exchange factor 4 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304431.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGTT TGCTGGTCCC TCCCTATGGT GTTATGGACA GCGGCCCAAA CGCTGACAGA 
ATGCCTGACA AAGAGAATCT GAGCAACAGT TCTCCTGGAC TATCACCCAA GCACTTAAAT 
AAGGTGCCGT CAGAGAAGAT ACTTCGGGCA GGAAAAGTGC TACGAAACAT CATTTTATCT 
CGAGCGCCTC ATATGATAAG AGACAGGAAA TACCACCTGA AGACGTACAG GCGGAGCTGC 
ATCGGCACTG AGCTCGTTGA TTGGCTGTTG CAGCACACAT CATGTGTTCA CTCTCGTGTT 
CAGGCTGTAG GGATGTGGCA GGTGCTGCTG GAGGAGGGAG TGCTTAACCA CGTGGACCAG 
GAGCCGACTT TCCAAGATAA ATATCTGTTT TATCGCTTTC TGGATGACGA AAGTGAGGAT 
TCAGGAATGC CGAGTGAGGA AGACAAGAAG GAGAGTGACG AAGAGCTGCA GGACACGCTT 
CTGCTGCTGT CTCAGATCGG CCCTGATGCT CATCTGCGGA TGATCCTGCG CAAACAACCC 
GGCCAGAGGA CGGTCGATGA CCTGGAGATC ATTTATGAGG AGCTGCTGCA CATTAAAGCC 
TTGTCCCATC TCTCCACCAC TGTGAAGAGG GAACTCGCTG GTGTTTTAAT ATTTGAGTCG 
CATGCGAAAG CAGGCACAGT GTTGTTCAAT CAGGGTGAAG AAGGGACGTC CTGGTACATC 
ATCCTGAAGG GCTCCGTCAA TGTGGTCATC TATGGAAAGG GTGTGGTGTG TACTCTACAT 
GAGGGTGATG ATTTCGGGAA GCTGGCTCTG GTGAATGACG CTCCTCGGGC CGCCTCCATC 
GTGCTGAGAG AAGACTTCTG CCACTTCCTC AGGGTGGATA AAGAAGACTT CAACAGAATC 
CTCAGGGATG TGGAGGCAAA CACTGTCCGT CTGAAGGAAC ATGAAGAGGA CGTGCTGGTT 
TTGGAGAAAG CTAACAACAG GAGCTCCAGT TCTTCTCCTC TCAAGTACAC TGTGATGTCA 
GGAACTCCAG AGAAGATTCT GGAGCACCTT CTGGAGACCA TGAAACTTGA TTGCCAATTC 
TCAGAGTCAG ATCCTGCTCT GGATGACTTT CTGCTCATGC ACTGTGTTTT TGTCCCGAAC 
AGTCAGCTGT GTCCACTGCT CATGACCCAT TATCACGCTC AGGCGTCCCA GGGCAGTGAA 
CAGGAGCGGA TGGATTACAC TCTCAATAAC AAGCGCAGGG TGGTGCGTCT GGTGTTACAG 
TGGGCCTCCA TCAACACGGA CCACCTGCAG GAGGAGGACA GCTCCTTCAG CTTCATACAG 
GAGTTTTATG AGATGGTGTG TGAAGACTCC AGGCTCACTC CAGCCCTGAA GGACCATCTG 
CCCGAACTGG AGAAGATCAT CAAGCAGAAC TCAGATGACG GCAGACCCTC TCAGAAAAAG 
CACAAAGTCC TGCTGCGTCA GTTCAGCACA GGGAACGACA GGCTTCAGAA GAGACAGCCC 
ATCAAAAGCA CAGACGAGAT CCTCTTCAAG GTGTACTGCA GTGATCACAC CTACTCTACT 
ATCCGTGTGC CCGTGTCTGC ATCCGTCAGA GAGGTCATCG CTGCTGTATC CGACAAACTG 
AGCTCAGGAG ACGATCTGCT GCTCATCCAT CTCAACTCAG CTGGAGATAA AGAGCTTCTG 
AAGCCGTCTG ATGTTTCCAT CTTCTCATCT CTCAGCATTA ATGGCCGTCT GTTCGTCTGC 
CCGAGGGACC AAATCGACTC TTTGACGCCG CTGCCCGAGC AGGAGGGACC GTCTGCAGGC 
TCCATGTCCA CGTTTGAGCT CATGAGCTCT AAAGATCTGG CCCATCAGAT GACCCTCTAC 
GACTGGGAGC TCTTCCACTG TGTGCATGAG CATGAGCTGA TCTACCACAC GTTCGGCCGA 
CATCATTTTA AGAAGACCAC CGCCAATCTG GACCTGTTCC TGCGGCGCTT CAATGAGGTG 
CAGCTGTGGG TGGTGACGGA GGTCTGTCTG TGTCCCGCGC TCAGCAAACG AGTGCAGCTC 
CTCAAGAAGT TCATCAAGAT CGCTGCACAC TGTAAGGAGT TTAAGAACCT GAACTCATTC 
TTTGCCATTA TCATGGGTCT GGGAAACCCT GCGGTCTGTA GACTCAGTCA GACATGGGAG 
AAGCTGCCCA GCAAATTCAA GAAGTTTTAT GGAGAGTTTG AAAACCTAAT GGATCCCTCC 
AGAAATCACA GAGCATATCG ACTTACAATG GCCAAGCTAG AACCCCCCAT CATCCCCTTC 
ATGCCCTTAT TAATTAAAGA CATGACTTTC ACACACGAGG GAAACAAGAC CTTCACTGAC 
CGACTGGTCA ACTTTGAGAA GATGAGAATG ATTGCAAACA CAGTCAGAAT CATAAGATAC 
TGCAGAAGTC AACCATTTGA TCAGGAAGCT CCGCAGGCGA CGGGTAAAAG CCATCAGGAC 
GTGCGCACTT ACGTCCGCCA CATCAGCGTG ATCGATAACC AGCGCAGTCT ATCACAGCTG 
TCCCACCGTC TAGAGCCCCG GCGGACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302706.1
CDS	163..2712
Translation	MAGLLVPPYGVMDSGPNADRMPDKENLSNSSPGLSPKHLNKVPSEKILRAGKVLRNIILSRAPHMIRDRKYHLKTYRRSCIGTELVDWLLQHTSCVHSRVQAVGMWQVLLEEGVLNHVDQEPTFQDKYLFYRFLDDESEDSGMPSEEDKKESDEELQDTLLLLSQIGPDAHLRMILRKQPGQRTVDDLEIIYEELLHIKALSHLSTTVKRELAGVLIFESHAKAGTVLFNQGEEGTSWYIILKGSVNVVIYGKGVVCTLHEGDDFGKLALVNDAPRAASIVLREDFCHFLRVDKEDFNRILRDVEANTVRLKEHEEDVLVLEKANNRSSSSSPLKYTVMSGTPEKILEHLLETMKLDCQFSESDPALDDFLLMHCVFVPNSQLCPLLMTHYHAQASQGSEQERMDYTLNNKRRVVRLVLQWASINTDHLQEEDSSFSFIQEFYEMVCEDSRLTPALKDHLPELEKIIKQNSDDGRPSQKKHKVLLRQFSTGNDRLQKRQPIKSTDEILFKVYCSDHTYSTIRVPVSASVREVIAAVSDKLSSGDDLLLIHLNSAGDKELLKPSDVSIFSSLSINGRLFVCPRDQIDSLTPLPEQEGPSAGSMSTFELMSSKDLAHQMTLYDWELFHCVHEHELIYHTFGRHHFKKTTANLDLFLRRFNEVQLWVVTEVCLCPALSKRVQLLKKFIKIAAHCKEFKNLNSFFAIIMGLGNPAVCRLSQTWEKLPSKFKKFYGEFENLMDPSRNHRAYRLTMAKLEPPIIPFMPLLIKDMTFTHEGNKTFTDRLVNFEKMRMIANTVRIIRYCRSQPFDQEAPQATGKSHQDVRTYVRHISVIDNQRSLSQLSHRLEPRRT

Target ORF information:

RefSeq Version	XM_009304431.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Rap guanine nucleotide exchange factor (GEF) 4 (rapgef4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304431.2

ORF Insert Sequence:

ATGGCTGGTT TGCTGGTCCC TCCCTATGGT GTTATGGACA GCGGCCCAAA CGCTGACAGA 
ATGCCTGACA AAGAGAATCT GAGCAACAGT TCTCCTGGAC TATCACCCAA GCACTTAAAT 
AAGGTGCCGT CAGAGAAGAT ACTTCGGGCA GGAAAAGTGC TACGAAACAT CATTTTATCT 
CGAGCGCCTC ATATGATAAG AGACAGGAAA TACCACCTGA AGACGTACAG GCGGAGCTGC 
ATCGGCACTG AGCTCGTTGA TTGGCTGTTG CAGCACACAT CATGTGTTCA CTCTCGTGTT 
CAGGCTGTAG GGATGTGGCA GGTGCTGCTG GAGGAGGGAG TGCTTAACCA CGTGGACCAG 
GAGCCGACTT TCCAAGATAA ATATCTGTTT TATCGCTTTC TGGATGACGA AAGTGAGGAT 
TCAGGAATGC CGAGTGAGGA AGACAAGAAG GAGAGTGACG AAGAGCTGCA GGACACGCTT 
CTGCTGCTGT CTCAGATCGG CCCTGATGCT CATCTGCGGA TGATCCTGCG CAAACAACCC 
GGCCAGAGGA CGGTCGATGA CCTGGAGATC ATTTATGAGG AGCTGCTGCA CATTAAAGCC 
TTGTCCCATC TCTCCACCAC TGTGAAGAGG GAACTCGCTG GTGTTTTAAT ATTTGAGTCG 
CATGCGAAAG CAGGCACAGT GTTGTTCAAT CAGGGTGAAG AAGGGACGTC CTGGTACATC 
ATCCTGAAGG GCTCCGTCAA TGTGGTCATC TATGGAAAGG GTGTGGTGTG TACTCTACAT 
GAGGGTGATG ATTTCGGGAA GCTGGCTCTG GTGAATGACG CTCCTCGGGC CGCCTCCATC 
GTGCTGAGAG AAGACTTCTG CCACTTCCTC AGGGTGGATA AAGAAGACTT CAACAGAATC 
CTCAGGGATG TGGAGGCAAA CACTGTCCGT CTGAAGGAAC ATGAAGAGGA CGTGCTGGTT 
TTGGAGAAAG CTAACAACAG GAGCTCCAGT TCTTCTCCTC TCAAGTACAC TGTGATGTCA 
GGAACTCCAG AGAAGATTCT GGAGCACCTT CTGGAGACCA TGAAACTTGA TTGCCAATTC 
TCAGAGTCAG ATCCTGCTCT GGATGACTTT CTGCTCATGC ACTGTGTTTT TGTCCCGAAC 
AGTCAGCTGT GTCCACTGCT CATGACCCAT TATCACGCTC AGGCGTCCCA GGGCAGTGAA 
CAGGAGCGGA TGGATTACAC TCTCAATAAC AAGCGCAGGG TGGTGCGTCT GGTGTTACAG 
TGGGCCTCCA TCAACACGGA CCACCTGCAG GAGGAGGACA GCTCCTTCAG CTTCATACAG 
GAGTTTTATG AGATGGTGTG TGAAGACTCC AGGCTCACTC CAGCCCTGAA GGACCATCTG 
CCCGAACTGG AGAAGATCAT CAAGCAGAAC TCAGATGACG GCAGACCCTC TCAGAAAAAG 
CACAAAGTCC TGCTGCGTCA GTTCAGCACA GGGAACGACA GGCTTCAGAA GAGACAGCCC 
ATCAAAAGCA CAGACGAGAT CCTCTTCAAG GTGTACTGCA GTGATCACAC CTACTCTACT 
ATCCGTGTGC CCGTGTCTGC ATCCGTCAGA GAGGTCATCG CTGCTGTATC CGACAAACTG 
AGCTCAGGAG ACGATCTGCT GCTCATCCAT CTCAACTCAG CTGGAGATAA AGAGCTTCTG 
AAGCCGTCTG ATGTTTCCAT CTTCTCATCT CTCAGCATTA ATGGCCGTCT GTTCGTCTGC 
CCGAGGGACC AAATCGACTC TTTGACGCCG CTGCCCGAGC AGGAGGGACC GTCTGCAGGC 
TCCATGTCCA CGTTTGAGCT CATGAGCTCT AAAGATCTGG CCCATCAGAT GACCCTCTAC 
GACTGGGAGC TCTTCCACTG TGTGCATGAG CATGAGCTGA TCTACCACAC GTTCGGCCGA 
CATCATTTTA AGAAGACCAC CGCCAATCTG GACCTGTTCC TGCGGCGCTT CAATGAGGTG 
CAGCTGTGGG TGGTGACGGA GGTCTGTCTG TGTCCCGCGC TCAGCAAACG AGTGCAGCTC 
CTCAAGAAGT TCATCAAGAT CGCTGCACAC TGTAAGGAGT TTAAGAACCT GAACTCATTC 
TTTGCCATTA TCATGGGTCT GGGAAACCCT GCGGTCTGTA GACTCAGTCA GACATGGGAG 
AAGCTGCCCA GCAAATTCAA GAAGTTTTAT GGAGAGTTTG AAAACCTAAT GGATCCCTCC 
AGAAATCACA GAGCATATCG ACTTACAATG GCCAAGCTAG AACCCCCCAT CATCCCCTTC 
ATGCCCTTAT TAATTAAAGA CATGACTTTC ACACACGAGG GAAACAAGAC CTTCACTGAC 
CGACTGGTCA ACTTTGAGAA GATGAGAATG ATTGCAAACA CAGTCAGAAT CATAAGATAC 
TGCAGAAGTC AACCATTTGA TCAGGAAGCT CCGCAGGCGA CGGGTAAAAG CCATCAGGAC 
GTGCGCACTT ACGTCCGCCA CATCAGCGTG ATCGATAACC AGCGCAGTCT ATCACAGCTG 
TCCCACCGTC TAGAGCCCCG GCGGACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rapgef4 ( XM_021478818.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334493.1 Danio rerio Rap guanine nucleotide exchange factor (GEF) 4 (rapgef4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa144032
Clone ID Related Accession (Same CDS sequence)	XM_021478818.1
Accession Version	XM_021478818.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	rap guanine nucleotide exchange factor 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTCAC CGAAGATGGT CGCGGCACAC ACCTCCAGCC ACCCGTCTGC ATCTGCTGAC 
TGGATCGTCT GTCTGGATAA GAGACCAGAG GAGCGTTCAG GGGAAGACGT GGACATAATC 
ATGGTGCGTC TGAAAAGTGT GAAGGCCTTC GAGAAGTTCC ACCCACCTCT TCTTCAGCAG 
ATCTGTCTGT GCGGCTTTTA TGAATGTCTG GAGAAAGGCA TCACTCTGTA CAGGCAGGGT 
GACATCGGCA CTAACTGGTA CACCGTCCTG TCCGGTTCTC TAGACGTCAA AGTCTCCGAA 
ACAGCAAACC ATCAGGATGC GGTCACCATC TGCACGCTGG GCATCGGCAC TGCTTTTGGA 
GAGTCTATCC TGGATAACAC ACCCCGCCAT GCCACCATCG TCACCCGAGA GTTCAGCGAG 
CTGCTGCGGA TCGAGCAGAA GGACTTCAGG AACCTGTGGG AGAATTATCG TCAGTGTATG 
GCTGGTTTGC TGGTCCCTCC CTATGGTGTT ATGGACAGCG GCCCAAACGC TGACAGAATG 
CCTGACAAAG AGAATCTGAG CAACAGTTCT CCTGGACTAT CACCCAAGCA CTTAAATAAG 
AAATCATTCA TTGAAAATCC CATCAAAGTT CATCCCAAAC CCATTAATCA GGTGCCGTCA 
GAGAAGATAC TTCGGGCAGG AAAAGTGCTA CGAAACATCA TTTTATCTCG AGCGCCTCAT 
ATGATAAGAG ACAGGAAATA CCACCTGAAG ACGTACAGGC GGAGCTGCAT CGGCACTGAG 
CTCGTTGATT GGCTGTTGCA GCACACATCA TGTGTTCACT CTCGTGTTCA GGCTGTAGGG 
ATGTGGCAGG TGCTGCTGGA GGAGGGAGTG CTTAACCACG TGGACCAGGA GCCGACTTTC 
CAAGATAAAT ATCTGTTTTA TCGCTTTCTG GATGACGAAA GTGAGGATTC AGGAATGCCG 
AGTGAGGAAG ACAAGAAGGA GAGTGACGAA GAGCTGCAGG ACACGCTTCT GCTGCTGTCT 
CAGATCGGCC CTGATGCTCA TCTGCGGATG ATCCTGCGCA AACAACCCGG CCAGAGGACG 
GTCGATGACC TGGAGATCAT TTATGAGGAG CTGCTGCACA TTAAAGCCTT GTCCCATCTC 
TCCACCACTG TGAAGAGGGA ACTCGCTGGT GTTTTAATAT TTGAGTCGCA TGCGAAAGCA 
GGCACAGTGT TGTTCAATCA GGGTGAAGAA GGGACGTCCT GGTACATCAT CCTGAAGGGC 
TCCGTCAATG TGGTCATCTA TGGAAAGGGT GTGGTGTGTA CTCTACATGA GGGTGATGAT 
TTCGGGAAGC TGGCTCTGGT GAATGACGCT CCTCGGGCCG CCTCCATCGT GCTGAGAGAA 
GACTTCTGCC ACTTCCTCAG GGTGGATAAA GAAGACTTCA ACAGAATCCT CAGGGATGTG 
GAGGCAAACA CTGTCCGTCT GAAGGAACAT GAAGAGGACG TGCTGGTTTT GGAGAAAGCT 
AACAACAGGA GCTCCAGTTC TTCTCCTCTC AAGTACACTG TGATGTCAGG AACTCCAGAG 
AAGATTCTGG AGCACCTTCT GGAGACCATG AAACTTGATT GCCAATTCTC AGAGTCAGAT 
CCTGCTCTGG ATGACTTTCT GCTCATGCAC TGTGTTTTTG TCCCGAACAG TCAGCTGTGT 
CCACTGCTCA TGACCCATTA TCACGCTCAG GCGTCCCAGG GCAGTGAACA GGAGCGGATG 
GATTACACTC TCAATAACAA GCGCAGGGTG GTGCGTCTGG TGTTACAGTG GGCCTCCATC 
AACACGGACC ACCTGCAGGA GGAGGACAGC TCCTTCAGCT TCATACAGGA GTTTTATGAG 
ATGGTGTGTG AAGACTCCAG GCTCACTCCA GCCCTGAAGG ACCATCTGCC CGAACTGGAG 
AAGATCATCA AGCAGAACTC AGATGACGGC AGACCCTCTC AGAAAAAGCA CAAAGTCCTG 
CTGCGTCAGT TCAGCACAGG GAACGACAGG CTTCAGAAGA GACAGCCCAT CAAAAGCACA 
GACGAGATCC TCTTCAAGGT GTACTGCAGT GATCACACCT ACTCTACTAT CCGTGTGCCC 
GTGTCTGCAT CCGTCAGAGA GGTCATCGCT GCTGTATCCG ACAAACTGAG CTCAGGAGAC 
GATCTGCTGC TCATCCATCT CAACTCAGCT GGAGATAAAG AGCTTCTGAA GCCGTCTGAT 
GTTTCCATCT TCTCATCTCT CAGCATTAAT GGCCGTCTGT TCGTCTGCCC GAGGGACCAA 
ATCGACTCTT TGACGCCGCT GCCCGAGCAG GAGGGACCGT CTGCAGGCTC CATGTCCACG 
TTTGAGCTCA TGAGCTCTAA AGATCTGGCC CATCAGATGA CCCTCTACGA CTGGGAGCTC 
TTCCACTGTG TGCATGAGCA TGAGCTGATC TACCACACGT TCGGCCGACA TCATTTTAAG 
AAGACCACCG CCAATCTGGA CCTGTTCCTG CGGCGCTTCA ATGAGGTGCA GCTGTGGGTG 
GTGACGGAGG TCTGTCTGTG TCCCGCGCTC AGCAAACGAG TGCAGCTCCT CAAGAAGTTC 
ATCAAGATCG CTGCACACTG TAAGGAGTTT AAGAACCTGA ACTCATTCTT TGCCATTATC 
ATGGGTCTGG GAAACCCTGC GGTCTGTAGA CTCAGTCAGA CATGGGAGAA GCTGCCCAGC 
AAATTCAAGA AGTTTTATGG AGAGTTTGAA AACCTAATGG ATCCCTCCAG AAATCACAGA 
GCATATCGAC TTACAATGGC CAAGCTAGAA CCCCCCATCA TCCCCTTCAT GCCCTTATTA 
ATTAAAGACA TGACTTTCAC ACACGAGGGA AACAAGACCT TCACTGACCG ACTGGTCAAC 
TTTGAGAAGA TGAGAATGAT TGCAAACACA GTCAGAATCA TAAGATACTG CAGAAGTCAA 
CCATTTGATC AGGAAGCTCC GCAGGCGACG GGTAAAAGCC ATCAGGACGT GCGCACTTAC 
GTCCGCCACA TCAGCGTGAT CGATAACCAG CGCAGTCTAT CACAGCTGTC CCACCGTCTA 
GAGCCCCGGC GGACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334493.1
CDS	79..3156
Translation	MISPKMVAAHTSSHPSASADWIVCLDKRPEERSGEDVDIIMVRLKSVKAFEKFHPPLLQQICLCGFYECLEKGITLYRQGDIGTNWYTVLSGSLDVKVSETANHQDAVTICTLGIGTAFGESILDNTPRHATIVTREFSELLRIEQKDFRNLWENYRQCMAGLLVPPYGVMDSGPNADRMPDKENLSNSSPGLSPKHLNKKSFIENPIKVHPKPINQVPSEKILRAGKVLRNIILSRAPHMIRDRKYHLKTYRRSCIGTELVDWLLQHTSCVHSRVQAVGMWQVLLEEGVLNHVDQEPTFQDKYLFYRFLDDESEDSGMPSEEDKKESDEELQDTLLLLSQIGPDAHLRMILRKQPGQRTVDDLEIIYEELLHIKALSHLSTTVKRELAGVLIFESHAKAGTVLFNQGEEGTSWYIILKGSVNVVIYGKGVVCTLHEGDDFGKLALVNDAPRAASIVLREDFCHFLRVDKEDFNRILRDVEANTVRLKEHEEDVLVLEKANNRSSSSSPLKYTVMSGTPEKILEHLLETMKLDCQFSESDPALDDFLLMHCVFVPNSQLCPLLMTHYHAQASQGSEQERMDYTLNNKRRVVRLVLQWASINTDHLQEEDSSFSFIQEFYEMVCEDSRLTPALKDHLPELEKIIKQNSDDGRPSQKKHKVLLRQFSTGNDRLQKRQPIKSTDEILFKVYCSDHTYSTIRVPVSASVREVIAAVSDKLSSGDDLLLIHLNSAGDKELLKPSDVSIFSSLSINGRLFVCPRDQIDSLTPLPEQEGPSAGSMSTFELMSSKDLAHQMTLYDWELFHCVHEHELIYHTFGRHHFKKTTANLDLFLRRFNEVQLWVVTEVCLCPALSKRVQLLKKFIKIAAHCKEFKNLNSFFAIIMGLGNPAVCRLSQTWEKLPSKFKKFYGEFENLMDPSRNHRAYRLTMAKLEPPIIPFMPLLIKDMTFTHEGNKTFTDRLVNFEKMRMIANTVRIIRYCRSQPFDQEAPQATGKSHQDVRTYVRHISVIDNQRSLSQLSHRLEPRRT

Target ORF information:

RefSeq Version	XM_021478818.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rap guanine nucleotide exchange factor (GEF) 4 (rapgef4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478818.1

ORF Insert Sequence:

ATGATTTCAC CGAAGATGGT CGCGGCACAC ACCTCCAGCC ACCCGTCTGC ATCTGCTGAC 
TGGATCGTCT GTCTGGATAA GAGACCAGAG GAGCGTTCAG GGGAAGACGT GGACATAATC 
ATGGTGCGTC TGAAAAGTGT GAAGGCCTTC GAGAAGTTCC ACCCACCTCT TCTTCAGCAG 
ATCTGTCTGT GCGGCTTTTA TGAATGTCTG GAGAAAGGCA TCACTCTGTA CAGGCAGGGT 
GACATCGGCA CTAACTGGTA CACCGTCCTG TCCGGTTCTC TAGACGTCAA AGTCTCCGAA 
ACAGCAAACC ATCAGGATGC GGTCACCATC TGCACGCTGG GCATCGGCAC TGCTTTTGGA 
GAGTCTATCC TGGATAACAC ACCCCGCCAT GCCACCATCG TCACCCGAGA GTTCAGCGAG 
CTGCTGCGGA TCGAGCAGAA GGACTTCAGG AACCTGTGGG AGAATTATCG TCAGTGTATG 
GCTGGTTTGC TGGTCCCTCC CTATGGTGTT ATGGACAGCG GCCCAAACGC TGACAGAATG 
CCTGACAAAG AGAATCTGAG CAACAGTTCT CCTGGACTAT CACCCAAGCA CTTAAATAAG 
AAATCATTCA TTGAAAATCC CATCAAAGTT CATCCCAAAC CCATTAATCA GGTGCCGTCA 
GAGAAGATAC TTCGGGCAGG AAAAGTGCTA CGAAACATCA TTTTATCTCG AGCGCCTCAT 
ATGATAAGAG ACAGGAAATA CCACCTGAAG ACGTACAGGC GGAGCTGCAT CGGCACTGAG 
CTCGTTGATT GGCTGTTGCA GCACACATCA TGTGTTCACT CTCGTGTTCA GGCTGTAGGG 
ATGTGGCAGG TGCTGCTGGA GGAGGGAGTG CTTAACCACG TGGACCAGGA GCCGACTTTC 
CAAGATAAAT ATCTGTTTTA TCGCTTTCTG GATGACGAAA GTGAGGATTC AGGAATGCCG 
AGTGAGGAAG ACAAGAAGGA GAGTGACGAA GAGCTGCAGG ACACGCTTCT GCTGCTGTCT 
CAGATCGGCC CTGATGCTCA TCTGCGGATG ATCCTGCGCA AACAACCCGG CCAGAGGACG 
GTCGATGACC TGGAGATCAT TTATGAGGAG CTGCTGCACA TTAAAGCCTT GTCCCATCTC 
TCCACCACTG TGAAGAGGGA ACTCGCTGGT GTTTTAATAT TTGAGTCGCA TGCGAAAGCA 
GGCACAGTGT TGTTCAATCA GGGTGAAGAA GGGACGTCCT GGTACATCAT CCTGAAGGGC 
TCCGTCAATG TGGTCATCTA TGGAAAGGGT GTGGTGTGTA CTCTACATGA GGGTGATGAT 
TTCGGGAAGC TGGCTCTGGT GAATGACGCT CCTCGGGCCG CCTCCATCGT GCTGAGAGAA 
GACTTCTGCC ACTTCCTCAG GGTGGATAAA GAAGACTTCA ACAGAATCCT CAGGGATGTG 
GAGGCAAACA CTGTCCGTCT GAAGGAACAT GAAGAGGACG TGCTGGTTTT GGAGAAAGCT 
AACAACAGGA GCTCCAGTTC TTCTCCTCTC AAGTACACTG TGATGTCAGG AACTCCAGAG 
AAGATTCTGG AGCACCTTCT GGAGACCATG AAACTTGATT GCCAATTCTC AGAGTCAGAT 
CCTGCTCTGG ATGACTTTCT GCTCATGCAC TGTGTTTTTG TCCCGAACAG TCAGCTGTGT 
CCACTGCTCA TGACCCATTA TCACGCTCAG GCGTCCCAGG GCAGTGAACA GGAGCGGATG 
GATTACACTC TCAATAACAA GCGCAGGGTG GTGCGTCTGG TGTTACAGTG GGCCTCCATC 
AACACGGACC ACCTGCAGGA GGAGGACAGC TCCTTCAGCT TCATACAGGA GTTTTATGAG 
ATGGTGTGTG AAGACTCCAG GCTCACTCCA GCCCTGAAGG ACCATCTGCC CGAACTGGAG 
AAGATCATCA AGCAGAACTC AGATGACGGC AGACCCTCTC AGAAAAAGCA CAAAGTCCTG 
CTGCGTCAGT TCAGCACAGG GAACGACAGG CTTCAGAAGA GACAGCCCAT CAAAAGCACA 
GACGAGATCC TCTTCAAGGT GTACTGCAGT GATCACACCT ACTCTACTAT CCGTGTGCCC 
GTGTCTGCAT CCGTCAGAGA GGTCATCGCT GCTGTATCCG ACAAACTGAG CTCAGGAGAC 
GATCTGCTGC TCATCCATCT CAACTCAGCT GGAGATAAAG AGCTTCTGAA GCCGTCTGAT 
GTTTCCATCT TCTCATCTCT CAGCATTAAT GGCCGTCTGT TCGTCTGCCC GAGGGACCAA 
ATCGACTCTT TGACGCCGCT GCCCGAGCAG GAGGGACCGT CTGCAGGCTC CATGTCCACG 
TTTGAGCTCA TGAGCTCTAA AGATCTGGCC CATCAGATGA CCCTCTACGA CTGGGAGCTC 
TTCCACTGTG TGCATGAGCA TGAGCTGATC TACCACACGT TCGGCCGACA TCATTTTAAG 
AAGACCACCG CCAATCTGGA CCTGTTCCTG CGGCGCTTCA ATGAGGTGCA GCTGTGGGTG 
GTGACGGAGG TCTGTCTGTG TCCCGCGCTC AGCAAACGAG TGCAGCTCCT CAAGAAGTTC 
ATCAAGATCG CTGCACACTG TAAGGAGTTT AAGAACCTGA ACTCATTCTT TGCCATTATC 
ATGGGTCTGG GAAACCCTGC GGTCTGTAGA CTCAGTCAGA CATGGGAGAA GCTGCCCAGC 
AAATTCAAGA AGTTTTATGG AGAGTTTGAA AACCTAATGG ATCCCTCCAG AAATCACAGA 
GCATATCGAC TTACAATGGC CAAGCTAGAA CCCCCCATCA TCCCCTTCAT GCCCTTATTA 
ATTAAAGACA TGACTTTCAC ACACGAGGGA AACAAGACCT TCACTGACCG ACTGGTCAAC 
TTTGAGAAGA TGAGAATGAT TGCAAACACA GTCAGAATCA TAAGATACTG CAGAAGTCAA 
CCATTTGATC AGGAAGCTCC GCAGGCGACG GGTAAAAGCC ATCAGGACGT GCGCACTTAC 
GTCCGCCACA TCAGCGTGAT CGATAACCAG CGCAGTCTAT CACAGCTGTC CCACCGTCTA 
GAGCCCCGGC GGACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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rapgef4 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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