CHKA cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	CHKA
Entrez Gene ID	558499
Full Name	choline kinase alpha
Synonyms	im:7143625,si:dkey-12e7.3,wu:fd46h01
General protein information	Preferred Names choline kinase alpha Names choline kinase alpha
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009293567.2	XP_009291842.1	choline kinase alpha isoform X2
NM_001044865.1	NP_001038330.1	choline kinase alpha
XM_005159105.4	XP_005159162.1	choline kinase alpha isoform X1
XM_009293567.1	XP_009291842.1	choline kinase alpha isoform X2
XM_005159105.3	XP_005159162.1	choline kinase alpha isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CHKA	XP_508602.3
Canis lupus familiaris (dog)	CHKA	XP_533210.3
Danio rerio (zebrafish)	chka	NP_001038330.1
Oryza sativa (rice)	Os01g0183000	NP_001042223.2
Homo sapiens (human)	CHKA	NP_001268.2
Rattus norvegicus (Norway rat)	Chka	NP_058823.1
Gallus gallus (chicken)	CHKA	XP_421045.3
Xenopus tropicalis (tropical clawed frog)	chka	NP_001011466.1
Bos taurus (cattle)	CHKA	XP_002699448.3
Mus musculus (house mouse)	Chka	NP_001258425.1
Caenorhabditis elegans (roundworm)	cka-2	NP_001024480.1
Arabidopsis thaliana (thale cress)	AT4G09760	NP_849350.1
Arabidopsis thaliana (thale cress)	CK1	NP_177315.1

Gene Ontology
Bibliography

Related articles in PubMed

Development of the Swimbladder Surfactant System and Biogenesis of Lysosome-Related Organelles Is Regulated by BLOS1 in Zebrafish.
Chen T, Song G, Yang H, Mao L, Cui Z, Huang K
Genetics208(3)1131-1146(2018 03)

Toxicogenomic responses of zebrafish embryos/larvae to tris(1,3-dichloro-2-propyl) phosphate (TDCPP) reveal possible molecular mechanisms of developmental toxicity.
Fu J, Han J, Zhou B, Gong Z, Santos EM, Huo X, Zheng W, Liu H, Yu H, Liu C
Environmental science & technology47(18)10574-82(2013 Sep)

Hematopoietic gene expression profile in zebrafish kidney marrow.
Song HD, Sun XJ, Deng M, Zhang GW, Zhou Y, Wu XY, Sheng Y, Chen Y, Ruan Z, Jiang CL, Fan HY, Zon LI, Kanki JP, Liu TX, Look AT, Chen Z
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CHKA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHKA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa03948	NM_001044865.1 Latest version!	Danio rerio choline kinase alpha (chka), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ODa23270	XM_005159105.4 Latest version!	Danio rerio choline kinase alpha (chka), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ODa23271	XM_009293567.2 Latest version!	Danio rerio choline kinase alpha (chka), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
ODa23271	XM_009293567.1	Danio rerio choline kinase alpha (chka), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ODa23270	XM_005159105.3	Danio rerio choline kinase alpha (chka), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHKA ( NM_001044865.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001038330.1 Danio rerio choline kinase alpha (chka), mRNA.

Price & Availability↑

CloneID	ODa03948
Clone ID Related Accession (Same CDS sequence)	NM_001044865.1
Accession Version	NM_001044865.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1533225600000
Organism	Danio rerio(zebrafish)
Product	choline kinase alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX571884.8. On or before Sep 1, 2006 this sequence version replaced XM_682586.1, XM_703726.1, XM_703727.1, XM_703728.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA3505370, SAMEA3505376 [ECO:0000348] ##Evidence-Data-END##

ATGAAGACTA AATTCATCAA TGGCGTGTCT TCTTCGCCTT CAATGTCTTT GGGTTTGCTG 
GTGAATGAAA GCGTCCTCGA AGTCCAGCCC GACAGTGTGA ACGACTGCAA AGATATTATC 
CGACCGGATG AGCCAGACCA GCAGACGAAG CATAAGGCGT ATCTGTGGTG TAGAGAATTC 
CTCCACGGAG CCTGGAAATC CATTGATGAA GAGGATTTTC AAATCTCAAT CATCAGAGGA 
GGCCTGAGCA ACAAACTGTT CCTCTGCGCT TTGCCAGAGA TGCAAGCAAG CCTTGGAGAT 
GAACCACGAA ATGTGCTCCT TCGCCTATAT GGAGAAATTC TGCAGGGAGC TGATGCCATG 
GTACTGGAGA GTGTGATGTT TGCTATCCTG GCCGAGAGGG AGCTTGGGCC CAAGCTCTAT 
GGCATCTTTC CACAAGGCAG GCTGGAACAG TTTGTCCCAA GTCGAAAACT GACCACAGAT 
GAGCTGAGCG TCCCTGGTAT AAATGCTGAG ATTGCTGAAA AGATCGCCAG ATTTCACGGA 
ATGAGAATGC CCTTCAACAA GGAGCCAAAG TGGCTTTTTG GCACCATGGA AAAGTACATG 
GATCAAGTGC TTCAGCTCAC CTTCACCAGG GAACCTCATC TGCGAAACTT CTCTCGCATT 
TTGAGCTACA ACTTGCCTCA AGAGATGGAC AATCTCAAGT GCCTACTTGA ATCTACCCCC 
TCTCCTGTTG TTTTCTGCCA CAATGACCTG CAAGAAGGAA ATATCTTACT TCTGAATGGC 
CGTGAGAACA CAGACAGACA GAGGCTCATG CTGATTGACT TCGAGTACAG CAGCTACAAC 
TACAGAGGAT TTGACATTGG AAATTTCTTC TGTGAGTGGA CCTACGACTA CACCTATGAC 
AAGTTCCCAT TCTTCACAAA CAACACCAAA AATTACCCCA CCAAAGCACA GCAGATGCAT 
ATCTTTCACA GTTATCTGTT GGAATCCGAT GCTGGATTTA AACACTTGAG TGAGGAGGAC 
CAGATGAAGC TCAAAGAAGA TATGCTAGTG GAGGTTAACA GGTTTGCTCT GGCCTCCCAC 
TTCTTTTGGG GATTGTGGTC CATGATCCAG GCCAAAATAT CTACAATTGA GTTCGGATAC 
ATGGAGTACG CCATGGCCAG GTTTGATGCC TACTTTGAGC TGAAGAGGAA GCTCGGGGTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001038330.1
CDS	143..1345
Translation	MKTKFINGVSSSPSMSLGLLVNESVLEVQPDSVNDCKDIIRPDEPDQQTKHKAYLWCREFLHGAWKSIDEEDFQISIIRGGLSNKLFLCALPEMQASLGDEPRNVLLRLYGEILQGADAMVLESVMFAILAERELGPKLYGIFPQGRLEQFVPSRKLTTDELSVPGINAEIAEKIARFHGMRMPFNKEPKWLFGTMEKYMDQVLQLTFTREPHLRNFSRILSYNLPQEMDNLKCLLESTPSPVVFCHNDLQEGNILLLNGRENTDRQRLMLIDFEYSSYNYRGFDIGNFFCEWTYDYTYDKFPFFTNNTKNYPTKAQQMHIFHSYLLESDAGFKHLSEEDQMKLKEDMLVEVNRFALASHFFWGLWSMIQAKISTIEFGYMEYAMARFDAYFELKRKLGV

Target ORF information:

RefSeq Version	NM_001044865.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio choline kinase alpha (chka), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001044865.1

ORF Insert Sequence:

ATGAAGACTA AATTCATCAA TGGCGTGTCT TCTTCGCCTT CAATGTCTTT GGGTTTGCTG 
GTGAATGAAA GCGTCCTCGA AGTCCAGCCC GACAGTGTGA ACGACTGCAA AGATATTATC 
CGACCGGATG AGCCAGACCA GCAGACGAAG CATAAGGCGT ATCTGTGGTG TAGAGAATTC 
CTCCACGGAG CCTGGAAATC CATTGATGAA GAGGATTTTC AAATCTCAAT CATCAGAGGA 
GGCCTGAGCA ACAAACTGTT CCTCTGCGCT TTGCCAGAGA TGCAAGCAAG CCTTGGAGAT 
GAACCACGAA ATGTGCTCCT TCGCCTATAT GGAGAAATTC TGCAGGGAGC TGATGCCATG 
GTACTGGAGA GTGTGATGTT TGCTATCCTG GCCGAGAGGG AGCTTGGGCC CAAGCTCTAT 
GGCATCTTTC CACAAGGCAG GCTGGAACAG TTTGTCCCAA GTCGAAAACT GACCACAGAT 
GAGCTGAGCG TCCCTGGTAT AAATGCTGAG ATTGCTGAAA AGATCGCCAG ATTTCACGGA 
ATGAGAATGC CCTTCAACAA GGAGCCAAAG TGGCTTTTTG GCACCATGGA AAAGTACATG 
GATCAAGTGC TTCAGCTCAC CTTCACCAGG GAACCTCATC TGCGAAACTT CTCTCGCATT 
TTGAGCTACA ACTTGCCTCA AGAGATGGAC AATCTCAAGT GCCTACTTGA ATCTACCCCC 
TCTCCTGTTG TTTTCTGCCA CAATGACCTG CAAGAAGGAA ATATCTTACT TCTGAATGGC 
CGTGAGAACA CAGACAGACA GAGGCTCATG CTGATTGACT TCGAGTACAG CAGCTACAAC 
TACAGAGGAT TTGACATTGG AAATTTCTTC TGTGAGTGGA CCTACGACTA CACCTATGAC 
AAGTTCCCAT TCTTCACAAA CAACACCAAA AATTACCCCA CCAAAGCACA GCAGATGCAT 
ATCTTTCACA GTTATCTGTT GGAATCCGAT GCTGGATTTA AACACTTGAG TGAGGAGGAC 
CAGATGAAGC TCAAAGAAGA TATGCTAGTG GAGGTTAACA GGTTTGCTCT GGCCTCCCAC 
TTCTTTTGGG GATTGTGGTC CATGATCCAG GCCAAAATAT CTACAATTGA GTTCGGATAC 
ATGGAGTACG CCATGGCCAG GTTTGATGCC TACTTTGAGC TGAAGAGGAA GCTCGGGGTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHKA ( XM_005159105.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005159162.1 Danio rerio choline kinase alpha (chka), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa23270
Clone ID Related Accession (Same CDS sequence)	XM_005159105.4 , XM_005159105.3
Accession Version	XM_005159105.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	choline kinase alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159105.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACTA AATTCATCAA TGGCGTGTCT TCTTCGCCTT CAATGTCTTT GGGTTTGCTG 
GTGAATGAAA GCGTCCTCGA AGTCCAGCCC GACAGTGTGA ACGACTGCAA AGATATTATC 
CGACCGGATG AGCCAGACCA GCAGACGAAG CATAAGGCGT ATCTGTGGTG TAGAGAATTC 
CTCCACGGAG CCTGGAAATC CATTGATGAA GAGGATTTTC AAATCTCAAT CATCAGAGGA 
GGCCTGAGCA ACAAACTGTT CCTCTGCGCT TTGCCAGAGA TGCAAGCAAG CCTTGGAGAT 
GAACCACGAA ATGTGCTCCT TCGCCTATAT GGAGAAATTC TGCAGATGTC CTGTAATAAA 
GGGGATTCCA GGCAATCAAA CACAGAAAAT CACTTTCAAG GAGCTGATGC CATGGTACTG 
GAGAGTGTGA TGTTTGCTAT CCTGGCCGAG AGGGAGCTTG GGCCCAAGCT CTATGGCATC 
TTTCCACAAG GCAGGCTGGA ACAGTTTGTC CCAAGTCGAA AACTGACCAC AGATGAGCTG 
AGCGTCCCTG GTATAAATGC TGAGATTGCT GAAAAGATCG CCAGATTTCA CGGAATGAGA 
ATGCCCTTCA ACAAGGAGCC AAAGTGGCTT TTTGGCACCA TGGAAAAGTA CATGGATCAA 
GTGCTTCAGC TCACCTTCAC CAGGGAACCT CATCTGCGAA ACTTCTCTCG CATTTTGAGC 
TACAACTTGC CTCAAGAGAT GGACAATCTC AAGTGCCTAC TTGAATCTAC CCCCTCTCCT 
GTTGTTTTCT GCCACAATGA CCTGCAAGAA GGAAATATCT TACTTCTGAA TGGCCGTGAG 
AACACAGACA GACAGAGGCT CATGCTGATT GACTTCGAGT ACAGCAGCTA CAACTACAGA 
GGATTTGACA TTGGAAATTT CTTCTGTGAG TGGACCTACG ACTACACCTA TGACAAGTTC 
CCATTCTTCA CAAACAACAC CAAAAATTAC CCCACCAAAG CACAGCAGAT GCATATCTTT 
CACAGTTATC TGTTGGAATC CGATGCTGGA TTTAAACACT TGAGTGAGGA GGACCAGATG 
AAGCTCAAAG AAGATATGCT AGTGGAGGTT AACAGGTTTG CTCTGGCCTC CCACTTCTTT 
TGGGGATTGT GGTCCATGAT CCAGGCCAAA ATATCTACAA TTGAGTTCGG ATACATGGAG 
TACGCCATGG CCAGGTTTGA TGCCTACTTT GAGCTGAAGA GGAAGCTCGG GGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159162.1
CDS	210..1466
Translation	MKTKFINGVSSSPSMSLGLLVNESVLEVQPDSVNDCKDIIRPDEPDQQTKHKAYLWCREFLHGAWKSIDEEDFQISIIRGGLSNKLFLCALPEMQASLGDEPRNVLLRLYGEILQMSCNKGDSRQSNTENHFQGADAMVLESVMFAILAERELGPKLYGIFPQGRLEQFVPSRKLTTDELSVPGINAEIAEKIARFHGMRMPFNKEPKWLFGTMEKYMDQVLQLTFTREPHLRNFSRILSYNLPQEMDNLKCLLESTPSPVVFCHNDLQEGNILLLNGRENTDRQRLMLIDFEYSSYNYRGFDIGNFFCEWTYDYTYDKFPFFTNNTKNYPTKAQQMHIFHSYLLESDAGFKHLSEEDQMKLKEDMLVEVNRFALASHFFWGLWSMIQAKISTIEFGYMEYAMARFDAYFELKRKLGV

Target ORF information:

RefSeq Version	XM_005159105.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio choline kinase alpha (chka), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159105.4

ORF Insert Sequence:

ATGAAGACTA AATTCATCAA TGGCGTGTCT TCTTCGCCTT CAATGTCTTT GGGTTTGCTG 
GTGAATGAAA GCGTCCTCGA AGTCCAGCCC GACAGTGTGA ACGACTGCAA AGATATTATC 
CGACCGGATG AGCCAGACCA GCAGACGAAG CATAAGGCGT ATCTGTGGTG TAGAGAATTC 
CTCCACGGAG CCTGGAAATC CATTGATGAA GAGGATTTTC AAATCTCAAT CATCAGAGGA 
GGCCTGAGCA ACAAACTGTT CCTCTGCGCT TTGCCAGAGA TGCAAGCAAG CCTTGGAGAT 
GAACCACGAA ATGTGCTCCT TCGCCTATAT GGAGAAATTC TGCAGATGTC CTGTAATAAA 
GGGGATTCCA GGCAATCAAA CACAGAAAAT CACTTTCAAG GAGCTGATGC CATGGTACTG 
GAGAGTGTGA TGTTTGCTAT CCTGGCCGAG AGGGAGCTTG GGCCCAAGCT CTATGGCATC 
TTTCCACAAG GCAGGCTGGA ACAGTTTGTC CCAAGTCGAA AACTGACCAC AGATGAGCTG 
AGCGTCCCTG GTATAAATGC TGAGATTGCT GAAAAGATCG CCAGATTTCA CGGAATGAGA 
ATGCCCTTCA ACAAGGAGCC AAAGTGGCTT TTTGGCACCA TGGAAAAGTA CATGGATCAA 
GTGCTTCAGC TCACCTTCAC CAGGGAACCT CATCTGCGAA ACTTCTCTCG CATTTTGAGC 
TACAACTTGC CTCAAGAGAT GGACAATCTC AAGTGCCTAC TTGAATCTAC CCCCTCTCCT 
GTTGTTTTCT GCCACAATGA CCTGCAAGAA GGAAATATCT TACTTCTGAA TGGCCGTGAG 
AACACAGACA GACAGAGGCT CATGCTGATT GACTTCGAGT ACAGCAGCTA CAACTACAGA 
GGATTTGACA TTGGAAATTT CTTCTGTGAG TGGACCTACG ACTACACCTA TGACAAGTTC 
CCATTCTTCA CAAACAACAC CAAAAATTAC CCCACCAAAG CACAGCAGAT GCATATCTTT 
CACAGTTATC TGTTGGAATC CGATGCTGGA TTTAAACACT TGAGTGAGGA GGACCAGATG 
AAGCTCAAAG AAGATATGCT AGTGGAGGTT AACAGGTTTG CTCTGGCCTC CCACTTCTTT 
TGGGGATTGT GGTCCATGAT CCAGGCCAAA ATATCTACAA TTGAGTTCGG ATACATGGAG 
TACGCCATGG CCAGGTTTGA TGCCTACTTT GAGCTGAAGA GGAAGCTCGG GGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHKA ( XM_005159105.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005159162.1 Danio rerio choline kinase alpha (chka), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa23270
Clone ID Related Accession (Same CDS sequence)	XM_005159105.4 , XM_005159105.3
Accession Version	XM_005159105.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	choline kinase alpha isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159105.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACTA AATTCATCAA TGGCGTGTCT TCTTCGCCTT CAATGTCTTT GGGTTTGCTG 
GTGAATGAAA GCGTCCTCGA AGTCCAGCCC GACAGTGTGA ACGACTGCAA AGATATTATC 
CGACCGGATG AGCCAGACCA GCAGACGAAG CATAAGGCGT ATCTGTGGTG TAGAGAATTC 
CTCCACGGAG CCTGGAAATC CATTGATGAA GAGGATTTTC AAATCTCAAT CATCAGAGGA 
GGCCTGAGCA ACAAACTGTT CCTCTGCGCT TTGCCAGAGA TGCAAGCAAG CCTTGGAGAT 
GAACCACGAA ATGTGCTCCT TCGCCTATAT GGAGAAATTC TGCAGATGTC CTGTAATAAA 
GGGGATTCCA GGCAATCAAA CACAGAAAAT CACTTTCAAG GAGCTGATGC CATGGTACTG 
GAGAGTGTGA TGTTTGCTAT CCTGGCCGAG AGGGAGCTTG GGCCCAAGCT CTATGGCATC 
TTTCCACAAG GCAGGCTGGA ACAGTTTGTC CCAAGTCGAA AACTGACCAC AGATGAGCTG 
AGCGTCCCTG GTATAAATGC TGAGATTGCT GAAAAGATCG CCAGATTTCA CGGAATGAGA 
ATGCCCTTCA ACAAGGAGCC AAAGTGGCTT TTTGGCACCA TGGAAAAGTA CATGGATCAA 
GTGCTTCAGC TCACCTTCAC CAGGGAACCT CATCTGCGAA ACTTCTCTCG CATTTTGAGC 
TACAACTTGC CTCAAGAGAT GGACAATCTC AAGTGCCTAC TTGAATCTAC CCCCTCTCCT 
GTTGTTTTCT GCCACAATGA CCTGCAAGAA GGAAATATCT TACTTCTGAA TGGCCGTGAG 
AACACAGACA GACAGAGGCT CATGCTGATT GACTTCGAGT ACAGCAGCTA CAACTACAGA 
GGATTTGACA TTGGAAATTT CTTCTGTGAG TGGACCTACG ACTACACCTA TGACAAGTTC 
CCATTCTTCA CAAACAACAC CAAAAATTAC CCCACCAAAG CACAGCAGAT GCATATCTTT 
CACAGTTATC TGTTGGAATC CGATGCTGGA TTTAAACACT TGAGTGAGGA GGACCAGATG 
AAGCTCAAAG AAGATATGCT AGTGGAGGTT AACAGGTTTG CTCTGGCCTC CCACTTCTTT 
TGGGGATTGT GGTCCATGAT CCAGGCCAAA ATATCTACAA TTGAGTTCGG ATACATGGAG 
TACGCCATGG CCAGGTTTGA TGCCTACTTT GAGCTGAAGA GGAAGCTCGG GGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159162.1
CDS	196..1452
Translation	MKTKFINGVSSSPSMSLGLLVNESVLEVQPDSVNDCKDIIRPDEPDQQTKHKAYLWCREFLHGAWKSIDEEDFQISIIRGGLSNKLFLCALPEMQASLGDEPRNVLLRLYGEILQMSCNKGDSRQSNTENHFQGADAMVLESVMFAILAERELGPKLYGIFPQGRLEQFVPSRKLTTDELSVPGINAEIAEKIARFHGMRMPFNKEPKWLFGTMEKYMDQVLQLTFTREPHLRNFSRILSYNLPQEMDNLKCLLESTPSPVVFCHNDLQEGNILLLNGRENTDRQRLMLIDFEYSSYNYRGFDIGNFFCEWTYDYTYDKFPFFTNNTKNYPTKAQQMHIFHSYLLESDAGFKHLSEEDQMKLKEDMLVEVNRFALASHFFWGLWSMIQAKISTIEFGYMEYAMARFDAYFELKRKLGV

Target ORF information:

RefSeq Version	XM_005159105.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio choline kinase alpha (chka), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159105.3

ORF Insert Sequence:

ATGAAGACTA AATTCATCAA TGGCGTGTCT TCTTCGCCTT CAATGTCTTT GGGTTTGCTG 
GTGAATGAAA GCGTCCTCGA AGTCCAGCCC GACAGTGTGA ACGACTGCAA AGATATTATC 
CGACCGGATG AGCCAGACCA GCAGACGAAG CATAAGGCGT ATCTGTGGTG TAGAGAATTC 
CTCCACGGAG CCTGGAAATC CATTGATGAA GAGGATTTTC AAATCTCAAT CATCAGAGGA 
GGCCTGAGCA ACAAACTGTT CCTCTGCGCT TTGCCAGAGA TGCAAGCAAG CCTTGGAGAT 
GAACCACGAA ATGTGCTCCT TCGCCTATAT GGAGAAATTC TGCAGATGTC CTGTAATAAA 
GGGGATTCCA GGCAATCAAA CACAGAAAAT CACTTTCAAG GAGCTGATGC CATGGTACTG 
GAGAGTGTGA TGTTTGCTAT CCTGGCCGAG AGGGAGCTTG GGCCCAAGCT CTATGGCATC 
TTTCCACAAG GCAGGCTGGA ACAGTTTGTC CCAAGTCGAA AACTGACCAC AGATGAGCTG 
AGCGTCCCTG GTATAAATGC TGAGATTGCT GAAAAGATCG CCAGATTTCA CGGAATGAGA 
ATGCCCTTCA ACAAGGAGCC AAAGTGGCTT TTTGGCACCA TGGAAAAGTA CATGGATCAA 
GTGCTTCAGC TCACCTTCAC CAGGGAACCT CATCTGCGAA ACTTCTCTCG CATTTTGAGC 
TACAACTTGC CTCAAGAGAT GGACAATCTC AAGTGCCTAC TTGAATCTAC CCCCTCTCCT 
GTTGTTTTCT GCCACAATGA CCTGCAAGAA GGAAATATCT TACTTCTGAA TGGCCGTGAG 
AACACAGACA GACAGAGGCT CATGCTGATT GACTTCGAGT ACAGCAGCTA CAACTACAGA 
GGATTTGACA TTGGAAATTT CTTCTGTGAG TGGACCTACG ACTACACCTA TGACAAGTTC 
CCATTCTTCA CAAACAACAC CAAAAATTAC CCCACCAAAG CACAGCAGAT GCATATCTTT 
CACAGTTATC TGTTGGAATC CGATGCTGGA TTTAAACACT TGAGTGAGGA GGACCAGATG 
AAGCTCAAAG AAGATATGCT AGTGGAGGTT AACAGGTTTG CTCTGGCCTC CCACTTCTTT 
TGGGGATTGT GGTCCATGAT CCAGGCCAAA ATATCTACAA TTGAGTTCGG ATACATGGAG 
TACGCCATGG CCAGGTTTGA TGCCTACTTT GAGCTGAAGA GGAAGCTCGG GGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHKA ( XM_009293567.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009291842.1 Danio rerio choline kinase alpha (chka), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa23271
Clone ID Related Accession (Same CDS sequence)	XM_009293567.2 , XM_009293567.1
Accession Version	XM_009293567.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	choline kinase alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293567.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCACG AAATGTGCTC CTTCGCCTAT ATGGAGAAAT TCTGCAGGCT TGCCAAAGGA 
GCACTCTTGT TTCCAGGTGA TCCCAGCATA CCATTCACGA TGTCCTGTAA TAAAGGGGAT 
TCCAGGCAAT CAAACACAGA AAATCACTTT CAAGGAGCTG ATGCCATGGT ACTGGAGAGT 
GTGATGTTTG CTATCCTGGC CGAGAGGGAG CTTGGGCCCA AGCTCTATGG CATCTTTCCA 
CAAGGCAGGC TGGAACAGTT TGTCCCAAGT CGAAAACTGA CCACAGATGA GCTGAGCGTC 
CCTGGTATAA ATGCTGAGAT TGCTGAAAAG ATCGCCAGAT TTCACGGAAT GAGAATGCCC 
TTCAACAAGG AGCCAAAGTG GCTTTTTGGC ACCATGGAAA AGTACATGGA TCAAGTGCTT 
CAGCTCACCT TCACCAGGGA ACCTCATCTG CGAAACTTCT CTCGCATTTT GAGCTACAAC 
TTGCCTCAAG AGATGGACAA TCTCAAGTGC CTACTTGAAT CTACCCCCTC TCCTGTTGTT 
TTCTGCCACA ATGACCTGCA AGAAGGAAAT ATCTTACTTC TGAATGGCCG TGAGAACACA 
GACAGACAGA GGCTCATGCT GATTGACTTC GAGTACAGCA GCTACAACTA CAGAGGATTT 
GACATTGGAA ATTTCTTCTG TGAGTGGACC TACGACTACA CCTATGACAA GTTCCCATTC 
TTCACAAACA ACACCAAAAA TTACCCCACC AAAGCACAGC AGATGCATAT CTTTCACAGT 
TATCTGTTGG AATCCGATGC TGGATTTAAA CACTTGAGTG AGGAGGACCA GATGAAGCTC 
AAAGAAGATA TGCTAGTGGA GGTTAACAGG TTTGCTCTGG CCTCCCACTT CTTTTGGGGA 
TTGTGGTCCA TGATCCAGGC CAAAATATCT ACAATTGAGT TCGGATACAT GGAGTACGCC 
ATGGCCAGGT TTGATGCCTA CTTTGAGCTG AAGAGGAAGC TCGGGGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291842.1
CDS	42..1052
Translation	MNHEMCSFAYMEKFCRLAKGALLFPGDPSIPFTMSCNKGDSRQSNTENHFQGADAMVLESVMFAILAERELGPKLYGIFPQGRLEQFVPSRKLTTDELSVPGINAEIAEKIARFHGMRMPFNKEPKWLFGTMEKYMDQVLQLTFTREPHLRNFSRILSYNLPQEMDNLKCLLESTPSPVVFCHNDLQEGNILLLNGRENTDRQRLMLIDFEYSSYNYRGFDIGNFFCEWTYDYTYDKFPFFTNNTKNYPTKAQQMHIFHSYLLESDAGFKHLSEEDQMKLKEDMLVEVNRFALASHFFWGLWSMIQAKISTIEFGYMEYAMARFDAYFELKRKLGV

Target ORF information:

RefSeq Version	XM_009293567.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio choline kinase alpha (chka), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293567.2

ORF Insert Sequence:

ATGAACCACG AAATGTGCTC CTTCGCCTAT ATGGAGAAAT TCTGCAGGCT TGCCAAAGGA 
GCACTCTTGT TTCCAGGTGA TCCCAGCATA CCATTCACGA TGTCCTGTAA TAAAGGGGAT 
TCCAGGCAAT CAAACACAGA AAATCACTTT CAAGGAGCTG ATGCCATGGT ACTGGAGAGT 
GTGATGTTTG CTATCCTGGC CGAGAGGGAG CTTGGGCCCA AGCTCTATGG CATCTTTCCA 
CAAGGCAGGC TGGAACAGTT TGTCCCAAGT CGAAAACTGA CCACAGATGA GCTGAGCGTC 
CCTGGTATAA ATGCTGAGAT TGCTGAAAAG ATCGCCAGAT TTCACGGAAT GAGAATGCCC 
TTCAACAAGG AGCCAAAGTG GCTTTTTGGC ACCATGGAAA AGTACATGGA TCAAGTGCTT 
CAGCTCACCT TCACCAGGGA ACCTCATCTG CGAAACTTCT CTCGCATTTT GAGCTACAAC 
TTGCCTCAAG AGATGGACAA TCTCAAGTGC CTACTTGAAT CTACCCCCTC TCCTGTTGTT 
TTCTGCCACA ATGACCTGCA AGAAGGAAAT ATCTTACTTC TGAATGGCCG TGAGAACACA 
GACAGACAGA GGCTCATGCT GATTGACTTC GAGTACAGCA GCTACAACTA CAGAGGATTT 
GACATTGGAA ATTTCTTCTG TGAGTGGACC TACGACTACA CCTATGACAA GTTCCCATTC 
TTCACAAACA ACACCAAAAA TTACCCCACC AAAGCACAGC AGATGCATAT CTTTCACAGT 
TATCTGTTGG AATCCGATGC TGGATTTAAA CACTTGAGTG AGGAGGACCA GATGAAGCTC 
AAAGAAGATA TGCTAGTGGA GGTTAACAGG TTTGCTCTGG CCTCCCACTT CTTTTGGGGA 
TTGTGGTCCA TGATCCAGGC CAAAATATCT ACAATTGAGT TCGGATACAT GGAGTACGCC 
ATGGCCAGGT TTGATGCCTA CTTTGAGCTG AAGAGGAAGC TCGGGGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHKA ( XM_009293567.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009291842.1 Danio rerio choline kinase alpha (chka), transcript variant X2, mRNA.

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CloneID	ODa23271
Clone ID Related Accession (Same CDS sequence)	XM_009293567.2 , XM_009293567.1
Accession Version	XM_009293567.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	choline kinase alpha isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCACG AAATGTGCTC CTTCGCCTAT ATGGAGAAAT TCTGCAGGCT TGCCAAAGGA 
GCACTCTTGT TTCCAGGTGA TCCCAGCATA CCATTCACGA TGTCCTGTAA TAAAGGGGAT 
TCCAGGCAAT CAAACACAGA AAATCACTTT CAAGGAGCTG ATGCCATGGT ACTGGAGAGT 
GTGATGTTTG CTATCCTGGC CGAGAGGGAG CTTGGGCCCA AGCTCTATGG CATCTTTCCA 
CAAGGCAGGC TGGAACAGTT TGTCCCAAGT CGAAAACTGA CCACAGATGA GCTGAGCGTC 
CCTGGTATAA ATGCTGAGAT TGCTGAAAAG ATCGCCAGAT TTCACGGAAT GAGAATGCCC 
TTCAACAAGG AGCCAAAGTG GCTTTTTGGC ACCATGGAAA AGTACATGGA TCAAGTGCTT 
CAGCTCACCT TCACCAGGGA ACCTCATCTG CGAAACTTCT CTCGCATTTT GAGCTACAAC 
TTGCCTCAAG AGATGGACAA TCTCAAGTGC CTACTTGAAT CTACCCCCTC TCCTGTTGTT 
TTCTGCCACA ATGACCTGCA AGAAGGAAAT ATCTTACTTC TGAATGGCCG TGAGAACACA 
GACAGACAGA GGCTCATGCT GATTGACTTC GAGTACAGCA GCTACAACTA CAGAGGATTT 
GACATTGGAA ATTTCTTCTG TGAGTGGACC TACGACTACA CCTATGACAA GTTCCCATTC 
TTCACAAACA ACACCAAAAA TTACCCCACC AAAGCACAGC AGATGCATAT CTTTCACAGT 
TATCTGTTGG AATCCGATGC TGGATTTAAA CACTTGAGTG AGGAGGACCA GATGAAGCTC 
AAAGAAGATA TGCTAGTGGA GGTTAACAGG TTTGCTCTGG CCTCCCACTT CTTTTGGGGA 
TTGTGGTCCA TGATCCAGGC CAAAATATCT ACAATTGAGT TCGGATACAT GGAGTACGCC 
ATGGCCAGGT TTGATGCCTA CTTTGAGCTG AAGAGGAAGC TCGGGGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291842.1
CDS	36..1046
Translation	MNHEMCSFAYMEKFCRLAKGALLFPGDPSIPFTMSCNKGDSRQSNTENHFQGADAMVLESVMFAILAERELGPKLYGIFPQGRLEQFVPSRKLTTDELSVPGINAEIAEKIARFHGMRMPFNKEPKWLFGTMEKYMDQVLQLTFTREPHLRNFSRILSYNLPQEMDNLKCLLESTPSPVVFCHNDLQEGNILLLNGRENTDRQRLMLIDFEYSSYNYRGFDIGNFFCEWTYDYTYDKFPFFTNNTKNYPTKAQQMHIFHSYLLESDAGFKHLSEEDQMKLKEDMLVEVNRFALASHFFWGLWSMIQAKISTIEFGYMEYAMARFDAYFELKRKLGV

Target ORF information:

RefSeq Version	XM_009293567.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio choline kinase alpha (chka), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293567.1

ORF Insert Sequence:

ATGAACCACG AAATGTGCTC CTTCGCCTAT ATGGAGAAAT TCTGCAGGCT TGCCAAAGGA 
GCACTCTTGT TTCCAGGTGA TCCCAGCATA CCATTCACGA TGTCCTGTAA TAAAGGGGAT 
TCCAGGCAAT CAAACACAGA AAATCACTTT CAAGGAGCTG ATGCCATGGT ACTGGAGAGT 
GTGATGTTTG CTATCCTGGC CGAGAGGGAG CTTGGGCCCA AGCTCTATGG CATCTTTCCA 
CAAGGCAGGC TGGAACAGTT TGTCCCAAGT CGAAAACTGA CCACAGATGA GCTGAGCGTC 
CCTGGTATAA ATGCTGAGAT TGCTGAAAAG ATCGCCAGAT TTCACGGAAT GAGAATGCCC 
TTCAACAAGG AGCCAAAGTG GCTTTTTGGC ACCATGGAAA AGTACATGGA TCAAGTGCTT 
CAGCTCACCT TCACCAGGGA ACCTCATCTG CGAAACTTCT CTCGCATTTT GAGCTACAAC 
TTGCCTCAAG AGATGGACAA TCTCAAGTGC CTACTTGAAT CTACCCCCTC TCCTGTTGTT 
TTCTGCCACA ATGACCTGCA AGAAGGAAAT ATCTTACTTC TGAATGGCCG TGAGAACACA 
GACAGACAGA GGCTCATGCT GATTGACTTC GAGTACAGCA GCTACAACTA CAGAGGATTT 
GACATTGGAA ATTTCTTCTG TGAGTGGACC TACGACTACA CCTATGACAA GTTCCCATTC 
TTCACAAACA ACACCAAAAA TTACCCCACC AAAGCACAGC AGATGCATAT CTTTCACAGT 
TATCTGTTGG AATCCGATGC TGGATTTAAA CACTTGAGTG AGGAGGACCA GATGAAGCTC 
AAAGAAGATA TGCTAGTGGA GGTTAACAGG TTTGCTCTGG CCTCCCACTT CTTTTGGGGA 
TTGTGGTCCA TGATCCAGGC CAAAATATCT ACAATTGAGT TCGGATACAT GGAGTACGCC 
ATGGCCAGGT TTGATGCCTA CTTTGAGCTG AAGAGGAAGC TCGGGGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Development of the Swimbladder Surfactant System and Biogenesis of Lysosome-Related Organelles Is Regulated by BLOS1 in Zebrafish.
Genetics208(3)1131-1146(2018 03)
Chen T,Song G,Yang H,Mao L,Cui Z,Huang K

Toxicogenomic responses of zebrafish embryos/larvae to tris(1,3-dichloro-2-propyl) phosphate (TDCPP) reveal possible molecular mechanisms of developmental toxicity.
Environmental science & technology47(18)10574-82(2013 Sep)
Fu J,Han J,Zhou B,Gong Z,Santos EM,Huo X,Zheng W,Liu H,Yu H,Liu C

Hematopoietic gene expression profile in zebrafish kidney marrow.
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)
Song HD,Sun XJ,Deng M,Zhang GW,Zhou Y,Wu XY,Sheng Y,Chen Y,Ruan Z,Jiang CL,Fan HY,Zon LI,Kanki JP,Liu TX,Look AT,Chen Z

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CHKA cDNA ORF clone, Danio rerio(Zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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