HERC2 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	HERC2
Entrez Gene ID	558479
Full Name	HECT and RLD domain containing E3 ubiquitin protein ligase 2
Synonyms	fd13h10,wu:fd13h10
General protein information	Preferred Names HECT and RLD domain containing E3 ubiquitin protein ligase 2 Names E3 ubiquitin-protein ligase HERC2 hect domain and RLD 2
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021471808.1	XP_021327483.1	E3 ubiquitin-protein ligase HERC2 isoform X3
XM_021477195.1	XP_021332870.1	E3 ubiquitin-protein ligase HERC2 isoform X1
XM_021477196.1	XP_021332871.1	E3 ubiquitin-protein ligase HERC2 isoform X2
XM_021471809.1	XP_021327484.1	E3 ubiquitin-protein ligase HERC2 isoform X4
XM_009302643.2	XP_009300918.2	E3 ubiquitin-protein ligase HERC2 isoform X2
XM_017356648.1	XP_017212137.1	E3 ubiquitin-protein ligase HERC2 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	HERC2	NP_004658.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011823	XP_552411.3
Macaca mulatta (Rhesus monkey)	HERC2	XP_001109429.2
Drosophila melanogaster (fruit fly)	HERC2	NP_608388.2
Pan troglodytes (chimpanzee)	LOC453257	XP_003952667.1
Bos taurus (cattle)	HERC2	NP_001193010.1
Rattus norvegicus (Norway rat)	Herc2	NP_001100990.1
Gallus gallus (chicken)	HERC2	NP_001239305.1
Canis lupus familiaris (dog)	HERC2	XP_536160.2
Mus musculus (house mouse)	Herc2	NP_034548.2
Danio rerio (zebrafish)	herc2	XP_686789.6
Xenopus tropicalis (tropical clawed frog)	herc2	XP_002937025.2

Gene Ontology

The following HERC2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HERC2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa144734	XM_021471808.1 Latest version!	Danio rerio HECT and RLD domain containing E3 ubiquitin protein ligase 2 (herc2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODa144735	XM_021471809.1 Latest version!	Danio rerio HECT and RLD domain containing E3 ubiquitin protein ligase 2 (herc2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODa143098	XM_021477195.1 Latest version!	Danio rerio HECT and RLD domain containing E3 ubiquitin protein ligase 2 (herc2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa143099	XM_021477196.1 Latest version!	Danio rerio HECT and RLD domain containing E3 ubiquitin protein ligase 2 (herc2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa59481	XM_017356648.1 Latest version!	Danio rerio HECT and RLD domain containing E3 ubiquitin protein ligase 2 (herc2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa59482	XM_009302643.2 Latest version!	Danio rerio HECT and RLD domain containing E3 ubiquitin protein ligase 2 (herc2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HERC2 ( XM_021471808.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021327483.1 Danio rerio HECT and RLD domain containing E3 ubiquitin protein ligase 2 (herc2), transcript variant X3, mRNA.

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CloneID	ODa144734
Clone ID Related Accession (Same CDS sequence)	XM_021471808.1
Accession Version	XM_021471808.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6033bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase HERC2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394658.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAGTCC ACAAACTGAA AATGATTGTA GATCCAGCTG ACAGCAGCTA CATGCCTTCA 
TTGGTGGTGG TATCAGGTGG AACCTCACTC AACAATTTGA TCGAGTTGAA GACCATCAAC 
ATCAATCCCA CTGACACTGT CGTCTCTCTA CTCAATGACT GCACAGAGTA TCACAGGTAC 
ATTGAGGTTG CTATAAAGCA GTGTCGAAGT TCAGGTATTG ACTGTAAGAT TCATGGCTTG 
AGTGCTGTGG GACGTGTCCG TGCCGAGGAT GAAGATCTTG CAACTGTACC TTTCCTGGCA 
TCTGACAACG AGGAAGAGGA AGATGAGAAG ACCGCGACTG GAAGTATGTC TCGAAAGAGA 
TTGTCAGGCC TCGAGTCTGC AGCCACTATC AGAACCAAAG TCTTTGTCTG GGGTCTTAAT 
GATAAGGACC AACTTGGAGG ACTTAAGGGG TCCAAGATCA AAGTCCCTTC ATTCTCAGAG 
ACTCTGTCTG CTCTGAATGT AGTGCAGGTG GCTGGTGGCT CTAAAAGTCT TTTTGCTGTG 
ACTGCTGAAG GTAAAGTATA TGCCTGTGGA GAGGCCACCA ATGGAAGACT TGGTTTAGGC 
CTCTCCAGTG GAACCATCCC AATTCCCAGA CAGATATCAG CTCTCAGCAA CTATGTGGTC 
AAGAAGGTTG CTGTGCATTC AGGTGGACGG CATGCCATGG CACTTACCGT GGATGGGAAA 
GTATTCTCTT GGGGTGAAGG AGACGATGGC AAGCTTGGAC ACTTCAGCCG AATGAACTGT 
GATAAGCCAA GGCTGATCGA GGCATTGAAA ACCAAACGCA TTCGAGATAT TGCGTGTGGC 
AGCTCTCACA GTGCCGCCAT CACCTCCAGT GGAGAGCTGT ACAGCTGGGG TCTGGGGGAA 
TATGGCCGTC TGGGCCATGG GGACAACACC ACCCAACTCA GACCCAAACT GGTCAAGGTG 
CTTTTAGGCC ATCGCGTTGT CCAGGTAGCT TGTGGAAGTA GAGATGCCCA AACTCTTGCA 
CTGACAGATG AAGGAATGGT GTTCTCATGG GGAGATGGAG ACTTTGGGAA ACTGGGCCGT 
GGTGGCAGCG AGGGATGTAA CATTCCTCAA AACATTGAGC GTCTGAATGG ACAGGGGGTC 
TGTCAAATTG AATGTGGAGC TCAATTCTCC CTCGCCCTCA CCAAATCTGG AGTTGTCTGG 
ACCTGGGGTA AAGGGGATTA CTTCAGACTG GGTCACGGCA CAGATGTTCA TGTCCGCAAA 
CCCCAGATGG TTGAAGGACT TAGGGGGAAG AAGATTGTTC ATGTGGCCGT TGGTGCTCTG 
CACTGCCTGG CTGTCACCGA GACTGGACAG GTGTTCGCGT GGGGGGATAA TGACCATGGG 
CAGCAGGGGA ATGGCACAAC CACAGTCAAC AGGAAGCCCA CATTGGTGCA GGGTCTAGAG 
GGTCAGAAAA TTACTCGTGT GGCCTGCGGT TCTTCTCATA GTGTTGCCTG GACCACAGTA 
GATGTCACTA CACCCTCCGT CCATGAGCCT GTGCTCTTCC AGACTGCTAG AGACTCTCTG 
GGAGCCTCTT ACCTTGGTGT GCCATCAGAT AGTGACCCGT CTTTACTGAG CAATAAGCTA 
AATGGCGTAA ACAATGTGAA GCCCAACAGG CCATCTCTGG CAAAGATCCT TCTCTCTCTG 
GATGGTAATC TGGCCAAACA GCAGGCCCTA TCCCATGTGC TGTCTTCCCT GCAGATCATG 
TATGCCAGAG ATGCAGTGGT GGGTGCATTA ATGCCATCCT GTATGATGCC TCTGGAGTGT 
CCCTCCAGCT CTCCGGTGCC CCCATCAGAC TGTTCATCAG CTTCCAGCCC CTGTGAGGCT 
GAGGGTCCTG CCCTGCCCTC AGAGAGTGAG GAGAGGGTCA GTCCGCACCC CTGGATGGAC 
AGCAAACGAG GAGAGGTAAC CACGTCTGAG GATGCCACTA CCCCATCTTC AGCAGTGACC 
CCTTCTACTG CCTCTGCCTC ATCTCGACCC TTCATACCAG TCACAGATGA TCCTGGTGCT 
GCCAGCATCA TTGCTGAGAC CATGACCAAA ACCAAAGAGG ACTCTGAGAA CCAGAGTAAA 
GCCGTGGGTC CAGAGCCACA GTATCTGGAT GAGTTCACCA GTCTCCTGAT CCCTGATGAC 
ACGCGTGTGA TGGTGGACCT GTTAAAACTA GCTGTATCTC TGCGCTCTGG TGATAAAGGA 
AAGGAAGTGC TGTCTGCTGT TCTGTCTGGC ATGGGTACAG CCTGCCCACA GGTGGCGGAT 
ATGCTTCTGG AGCTGTGTGT GACTGAGTTG GAGGACGTGG CGACTGACTG TCAGAGTGGA 
CGGCTTTCCT CTCAACCTGT GGTGGTGGAA AGCAGCCATC CATATATTGA CGATACATCC 
ACCAGCGGAA CTGTCAAAAT ACCAGGTGCT GAGGGTTTGC GGGTTGAGTT TGATCGCCAG 
TGCTCAACAG AAAGGAGACA TGACCCTCTT ACCATCATGG ATGGAGCCAA TAGAATTGTG 
TCTGTTCGAT CAGGTCGTGA GTGGTCTGAT TGGTCCAGTG AATTGAGAAT TCCAGGAGAC 
GAGCTCAAAT GGAAGTTCAC CAGTGACGGC TCTGTGAACG GCTGGGGCTG GCGCTTCACA 
GTCTATCCCA TCATGCCTGC GGCTGGTCCT AAAGATCTTC TGTCAGACCG CTGCATTTTG 
TCTTGCCCTT CAATGGATCT AGTAACATGT CTGCTAGACT TTCGTCTGAA CTTTGCCTCA 
AACCGCAGCA TTGTGCCCAG ATTAGCAGCC TCACTTGCTG CTTGTGCGCA GCTCAGCGCC 
TTGGCGGCTG GCCATCGTAT GTGGGCTCTG CAGAGGTTAC GCAAGCTCTT AACTACTGAA 
TACGGCCAAT CCATTAACAT CAACCGCCTC TTAGGAGACA CCGAAGGAGA GGCTCGCTCT 
ATGAGTTTTA CAGGAAGTGC ATTAGCTGCT CTGGTGAAAG GACTTCCTGA GGCATTACAG 
AGACAGTATG AGTACGAGGA GCCCATAGTT AGGGGTGGCA AACAGTTACT CCACAGCCCG 
TTCTTCAAGG TGTTGGTGGC GCTGGCGTGT GATCTGGAGT TGGACACTCT TCCATGTTGT 
GCTGAGACAC ACAAGTGGGC GTGGTTTCGA CGCTATTGCA CCGCCTCCAG AGTGTCAGTG 
GCTCTGGACA AGAGGAGCCC TTTGCCCCGC CCTTTTCTGG ATGAGGTAGT GGCAAAGAAA 
ATCCGTGAGC TGATGGCAGA TCATGAGAAC ATGAACACGC TTCATGAGAG CCATGACTTG 
TTTAAGAGGG AGCAGGATGA GCAGTTGGTT CAGTGGATGA ACAGACGACC AGATGATTGG 
ACACTCTCTG CGGGTGGCAG TGGTACAATA TACGGCTGGG GGCACAATCA CCGGGGTCAG 
TTAGGTGGCA TTGAAGGAGC CAAAGTAAAG GTTCCCACAC CAACAGAAGC TCTAGCCACA 
CTGCGGCCAG TTCAGCTTAT TGGAGGAGAG CAAACACTGT TTGCCGTCAC TGCTGATGGG 
AAGCTGTATG CCACAGGATA TGGTGCCGGT GGTCGACTGG GAATAGGAGG GACAGAGTCC 
GTCTCCACTC CGACCCTGCT GGAGTCCATC CAGCATGTTT TCATCAGAAA GGTGGCTGTG 
AACTCTGGAG GGAAACACTG TTTGGCTCTT TCCTCTGAAG GAGAGCTGTA CTCCTGGGGG 
GAGGCCGAAG ATGGAAAACT GGGCCATGGA AACAGAAGCC CATGTGATCG TCCACGCGTG 
ATTGAGTCTC TCAGAGGGGT GGAAGTGGTG GACATTGCTG CCGGTGGGGC TCACAGTGCC 
TGCATTACTG CCAGTGGAGA GCTCTACACA TGGGGCAAAG GTCGCTATGG GCGCCTGGGT 
CATGGAGACA GCGAAGACCA ACTTAAACCA AAACTGGTGG AGGCTCTTCA GGGTCACCGG 
GTGATAGATG TGGCATGTGG CAGTGGAGAT GCACAGACCC TCTGTCTGAC TGATGATGAC 
ATGGTATGGT CGTGGGGCGA TGGAGACTAT GGCAAATTGG GCCGAGGAGG TAGTGATGGA 
TGTAAAGTGC CGATGAAGAT CGATTCATTA ACCGGATTGG GTGTTGTGAA AGTGGAATGT 
GGCTCTCAGT TTTCTGTGGC CCTTACCAAG TCTGGAGCAG TTTACACATG GGGGAAGGGT 
GACTATCACA GACTGGGACA CGGCTCAGAT GATCATGTTC GCAGACCCCG GCAGGTCCAG 
GGTTTACAAG GCAAAAAGGT CATCGCAATA GCAACTGGTT CCCTTCACTG CGTTTGCTGC 
ACGGAGGACG GGGAAGTGTA CACATGGGGT GACAATGATG AGGGTCAGCT TGGGGACGGT 
ACGACCAATG CTATTCAGAG ACCCAGGCTG GTGGCAGCGC TGCAGGGCAA GAAGATTAAT 
CGTGTGGCCT GTGGTTCAGC ACATACACTT GCCTGGTCCA CTAGTAAACC AACCAATGCT 
GGAAAACTCC CTGCTCAGGT TCCAATGGAG TACAACCACC TGCAAGAAAT CGCCATCATC 
GGCCTGAGGA ATCGGTTACT GCTACTGCAC CACATCTCTG AGCTCTTCTG CCCCTGCATC 
CCAATGTTTG ACTTAGAAGG ACGCCTGGGT GAAACTGGAC ATGGTGTGTC TGTTGGCTTT 
GACACGCTCC GTGGAATCCT CATATCACAG GGCAAGGAGG CAGCATTCCG CAAAGTGGTG 
CAGGCTACCA TGGTCAGAGA CAGGCAGCAT GGGCCAGTGG TTGAGCTGAA CAGGATACAG 
GTCAAGCGTT CACGTAGTAA AGGAGGTCTG GCAGGGCCGG ATGGCACCAA GTCAGTATTT 
GGGCAGATGT GTGCTAAGAT GAGTTCTTTC AGTCCAGACA GTCTGCTGCT GCCACACCGC 
GTCTGGAAGG TCAAGTTTGT GGGTGAGTCG GTGGATGACT GTGGTGGCGG ATACAGTGAA 
TCTATCGCAG AAATGTGCGA GGAGCTTCAG AATGGCCTCA CTCCTCTGCT CATCGTCACT 
CCTAATGGCA GAGACGAGTC AGGAGCGAAC AGAGATTGCT TTCTGCTCAA CCCAGCTGCC 
AAATCCCCTC TGCACATCAG CATGTTCCGC TTCCTAGGGG TGCTCCTGGG CATAGCGATC 
CGAACAGGAA GTCCTCTTAG TCTGTATCTG GCCGAACCTG TGTGGAAACA GCTGGCAGGC 
ATGAACCTGA CCATTGCTGA TCTCAGTGAG GTGGACAAGG ACTTCATCCC CGGTCTGATG 
TATATTAGAG ATAACGAGGC GTCTGCGGAG GAGTTTGAGG CGATGCCTCT TCCCTTCACT 
GTGCCCAGTG CCTGCGGACA AGAAGTCCAG CTCAGCTCCA AACATTCCCA CATCAGCCTT 
GAAAACCGCA GTGAATATGT GCGGCTAGCA ATAAATTACA GGCTGCATGA GTTTGATGAG 
CAGGTGTCTG CTGTAAGAGA AGGTATGGCG CGTGTGGTTC CGGTACCGCT CCTCTCTCTA 
TTTACCGGCT ATGAGCTGGA GACCATGGTG TGCGGAAGTC CGGACATCCC TCTGCATCTG 
CTCAAATCAG TGGCCACATA TAAAGGAGTA GAGCCCACTG CACCGCTCAT CCAGTGGTTC 
TGGGAGGTGA TGGAGTCTTT CTCTAACACA GAACGCTCGC TCTTCCTCAG GTTCGTCTGG 
GGCCGCACGC GTCTTCCCAG GACCATCGCA GACTTCCGTG GACGAGACTT TGTAGTGCAG 
GTGCTGGATA AATATAACCC TCCCGACCAC TTCCTCCCCG AGTCCTACAC CTGCTTCTTC 
CTGCTCAAGC TGCCTCGCTA CTCCTGCAAG CTAGTCTTGG AGGAGAAACT CAAATACGCC 
ATTCACTTCT GCAAGTCCAT CGACACTGAT GACTACGCAC GGATCGCTCT GAGCGGAGAA 
CCGGCCGCAG ACGACAGCAG TGATGACTCG GACAATGAAG ACGCAGACTC ATTCGCTTCT 
GACTCCACAC AGGATTACCT GACAGGCCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327483.1
CDS	27..6059
Translation	MLVHKLKMIVDPADSSYMPSLVVVSGGTSLNNLIELKTININPTDTVVSLLNDCTEYHRYIEVAIKQCRSSGIDCKIHGLSAVGRVRAEDEDLATVPFLASDNEEEEDEKTATGSMSRKRLSGLESAATIRTKVFVWGLNDKDQLGGLKGSKIKVPSFSETLSALNVVQVAGGSKSLFAVTAEGKVYACGEATNGRLGLGLSSGTIPIPRQISALSNYVVKKVAVHSGGRHAMALTVDGKVFSWGEGDDGKLGHFSRMNCDKPRLIEALKTKRIRDIACGSSHSAAITSSGELYSWGLGEYGRLGHGDNTTQLRPKLVKVLLGHRVVQVACGSRDAQTLALTDEGMVFSWGDGDFGKLGRGGSEGCNIPQNIERLNGQGVCQIECGAQFSLALTKSGVVWTWGKGDYFRLGHGTDVHVRKPQMVEGLRGKKIVHVAVGALHCLAVTETGQVFAWGDNDHGQQGNGTTTVNRKPTLVQGLEGQKITRVACGSSHSVAWTTVDVTTPSVHEPVLFQTARDSLGASYLGVPSDSDPSLLSNKLNGVNNVKPNRPSLAKILLSLDGNLAKQQALSHVLSSLQIMYARDAVVGALMPSCMMPLECPSSSPVPPSDCSSASSPCEAEGPALPSESEERVSPHPWMDSKRGEVTTSEDATTPSSAVTPSTASASSRPFIPVTDDPGAASIIAETMTKTKEDSENQSKAVGPEPQYLDEFTSLLIPDDTRVMVDLLKLAVSLRSGDKGKEVLSAVLSGMGTACPQVADMLLELCVTELEDVATDCQSGRLSSQPVVVESSHPYIDDTSTSGTVKIPGAEGLRVEFDRQCSTERRHDPLTIMDGANRIVSVRSGREWSDWSSELRIPGDELKWKFTSDGSVNGWGWRFTVYPIMPAAGPKDLLSDRCILSCPSMDLVTCLLDFRLNFASNRSIVPRLAASLAACAQLSALAAGHRMWALQRLRKLLTTEYGQSININRLLGDTEGEARSMSFTGSALAALVKGLPEALQRQYEYEEPIVRGGKQLLHSPFFKVLVALACDLELDTLPCCAETHKWAWFRRYCTASRVSVALDKRSPLPRPFLDEVVAKKIRELMADHENMNTLHESHDLFKREQDEQLVQWMNRRPDDWTLSAGGSGTIYGWGHNHRGQLGGIEGAKVKVPTPTEALATLRPVQLIGGEQTLFAVTADGKLYATGYGAGGRLGIGGTESVSTPTLLESIQHVFIRKVAVNSGGKHCLALSSEGELYSWGEAEDGKLGHGNRSPCDRPRVIESLRGVEVVDIAAGGAHSACITASGELYTWGKGRYGRLGHGDSEDQLKPKLVEALQGHRVIDVACGSGDAQTLCLTDDDMVWSWGDGDYGKLGRGGSDGCKVPMKIDSLTGLGVVKVECGSQFSVALTKSGAVYTWGKGDYHRLGHGSDDHVRRPRQVQGLQGKKVIAIATGSLHCVCCTEDGEVYTWGDNDEGQLGDGTTNAIQRPRLVAALQGKKINRVACGSAHTLAWSTSKPTNAGKLPAQVPMEYNHLQEIAIIGLRNRLLLLHHISELFCPCIPMFDLEGRLGETGHGVSVGFDTLRGILISQGKEAAFRKVVQATMVRDRQHGPVVELNRIQVKRSRSKGGLAGPDGTKSVFGQMCAKMSSFSPDSLLLPHRVWKVKFVGESVDDCGGGYSESIAEMCEELQNGLTPLLIVTPNGRDESGANRDCFLLNPAAKSPLHISMFRFLGVLLGIAIRTGSPLSLYLAEPVWKQLAGMNLTIADLSEVDKDFIPGLMYIRDNEASAEEFEAMPLPFTVPSACGQEVQLSSKHSHISLENRSEYVRLAINYRLHEFDEQVSAVREGMARVVPVPLLSLFTGYELETMVCGSPDIPLHLLKSVATYKGVEPTAPLIQWFWEVMESFSNTERSLFLRFVWGRTRLPRTIADFRGRDFVVQVLDKYNPPDHFLPESYTCFFLLKLPRYSCKLVLEEKLKYAIHFCKSIDTDDYARIALSGEPAADDSSDDSDNEDADSFASDSTQDYLTGH

Target ORF information:

RefSeq Version	XM_021471808.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio HECT and RLD domain containing E3 ubiquitin protein ligase 2 (herc2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471808.1

ORF Insert Sequence:

ATGTTAGTCC ACAAACTGAA AATGATTGTA GATCCAGCTG ACAGCAGCTA CATGCCTTCA 
TTGGTGGTGG TATCAGGTGG AACCTCACTC AACAATTTGA TCGAGTTGAA GACCATCAAC 
ATCAATCCCA CTGACACTGT CGTCTCTCTA CTCAATGACT GCACAGAGTA TCACAGGTAC 
ATTGAGGTTG CTATAAAGCA GTGTCGAAGT TCAGGTATTG ACTGTAAGAT TCATGGCTTG 
AGTGCTGTGG GACGTGTCCG TGCCGAGGAT GAAGATCTTG CAACTGTACC TTTCCTGGCA 
TCTGACAACG AGGAAGAGGA AGATGAGAAG ACCGCGACTG GAAGTATGTC TCGAAAGAGA 
TTGTCAGGCC TCGAGTCTGC AGCCACTATC AGAACCAAAG TCTTTGTCTG GGGTCTTAAT 
GATAAGGACC AACTTGGAGG ACTTAAGGGG TCCAAGATCA AAGTCCCTTC ATTCTCAGAG 
ACTCTGTCTG CTCTGAATGT AGTGCAGGTG GCTGGTGGCT CTAAAAGTCT TTTTGCTGTG 
ACTGCTGAAG GTAAAGTATA TGCCTGTGGA GAGGCCACCA ATGGAAGACT TGGTTTAGGC 
CTCTCCAGTG GAACCATCCC AATTCCCAGA CAGATATCAG CTCTCAGCAA CTATGTGGTC 
AAGAAGGTTG CTGTGCATTC AGGTGGACGG CATGCCATGG CACTTACCGT GGATGGGAAA 
GTATTCTCTT GGGGTGAAGG AGACGATGGC AAGCTTGGAC ACTTCAGCCG AATGAACTGT 
GATAAGCCAA GGCTGATCGA GGCATTGAAA ACCAAACGCA TTCGAGATAT TGCGTGTGGC 
AGCTCTCACA GTGCCGCCAT CACCTCCAGT GGAGAGCTGT ACAGCTGGGG TCTGGGGGAA 
TATGGCCGTC TGGGCCATGG GGACAACACC ACCCAACTCA GACCCAAACT GGTCAAGGTG 
CTTTTAGGCC ATCGCGTTGT CCAGGTAGCT TGTGGAAGTA GAGATGCCCA AACTCTTGCA 
CTGACAGATG AAGGAATGGT GTTCTCATGG GGAGATGGAG ACTTTGGGAA ACTGGGCCGT 
GGTGGCAGCG AGGGATGTAA CATTCCTCAA AACATTGAGC GTCTGAATGG ACAGGGGGTC 
TGTCAAATTG AATGTGGAGC TCAATTCTCC CTCGCCCTCA CCAAATCTGG AGTTGTCTGG 
ACCTGGGGTA AAGGGGATTA CTTCAGACTG GGTCACGGCA CAGATGTTCA TGTCCGCAAA 
CCCCAGATGG TTGAAGGACT TAGGGGGAAG AAGATTGTTC ATGTGGCCGT TGGTGCTCTG 
CACTGCCTGG CTGTCACCGA GACTGGACAG GTGTTCGCGT GGGGGGATAA TGACCATGGG 
CAGCAGGGGA ATGGCACAAC CACAGTCAAC AGGAAGCCCA CATTGGTGCA GGGTCTAGAG 
GGTCAGAAAA TTACTCGTGT GGCCTGCGGT TCTTCTCATA GTGTTGCCTG GACCACAGTA 
GATGTCACTA CACCCTCCGT CCATGAGCCT GTGCTCTTCC AGACTGCTAG AGACTCTCTG 
GGAGCCTCTT ACCTTGGTGT GCCATCAGAT AGTGACCCGT CTTTACTGAG CAATAAGCTA 
AATGGCGTAA ACAATGTGAA GCCCAACAGG CCATCTCTGG CAAAGATCCT TCTCTCTCTG 
GATGGTAATC TGGCCAAACA GCAGGCCCTA TCCCATGTGC TGTCTTCCCT GCAGATCATG 
TATGCCAGAG ATGCAGTGGT GGGTGCATTA ATGCCATCCT GTATGATGCC TCTGGAGTGT 
CCCTCCAGCT CTCCGGTGCC CCCATCAGAC TGTTCATCAG CTTCCAGCCC CTGTGAGGCT 
GAGGGTCCTG CCCTGCCCTC AGAGAGTGAG GAGAGGGTCA GTCCGCACCC CTGGATGGAC 
AGCAAACGAG GAGAGGTAAC CACGTCTGAG GATGCCACTA CCCCATCTTC AGCAGTGACC 
CCTTCTACTG CCTCTGCCTC ATCTCGACCC TTCATACCAG TCACAGATGA TCCTGGTGCT 
GCCAGCATCA TTGCTGAGAC CATGACCAAA ACCAAAGAGG ACTCTGAGAA CCAGAGTAAA 
GCCGTGGGTC CAGAGCCACA GTATCTGGAT GAGTTCACCA GTCTCCTGAT CCCTGATGAC 
ACGCGTGTGA TGGTGGACCT GTTAAAACTA GCTGTATCTC TGCGCTCTGG TGATAAAGGA 
AAGGAAGTGC TGTCTGCTGT TCTGTCTGGC ATGGGTACAG CCTGCCCACA GGTGGCGGAT 
ATGCTTCTGG AGCTGTGTGT GACTGAGTTG GAGGACGTGG CGACTGACTG TCAGAGTGGA 
CGGCTTTCCT CTCAACCTGT GGTGGTGGAA AGCAGCCATC CATATATTGA CGATACATCC 
ACCAGCGGAA CTGTCAAAAT ACCAGGTGCT GAGGGTTTGC GGGTTGAGTT TGATCGCCAG 
TGCTCAACAG AAAGGAGACA TGACCCTCTT ACCATCATGG ATGGAGCCAA TAGAATTGTG 
TCTGTTCGAT CAGGTCGTGA GTGGTCTGAT TGGTCCAGTG AATTGAGAAT TCCAGGAGAC 
GAGCTCAAAT GGAAGTTCAC CAGTGACGGC TCTGTGAACG GCTGGGGCTG GCGCTTCACA 
GTCTATCCCA TCATGCCTGC GGCTGGTCCT AAAGATCTTC TGTCAGACCG CTGCATTTTG 
TCTTGCCCTT CAATGGATCT AGTAACATGT CTGCTAGACT TTCGTCTGAA CTTTGCCTCA 
AACCGCAGCA TTGTGCCCAG ATTAGCAGCC TCACTTGCTG CTTGTGCGCA GCTCAGCGCC 
TTGGCGGCTG GCCATCGTAT GTGGGCTCTG CAGAGGTTAC GCAAGCTCTT AACTACTGAA 
TACGGCCAAT CCATTAACAT CAACCGCCTC TTAGGAGACA CCGAAGGAGA GGCTCGCTCT 
ATGAGTTTTA CAGGAAGTGC ATTAGCTGCT CTGGTGAAAG GACTTCCTGA GGCATTACAG 
AGACAGTATG AGTACGAGGA GCCCATAGTT AGGGGTGGCA AACAGTTACT CCACAGCCCG 
TTCTTCAAGG TGTTGGTGGC GCTGGCGTGT GATCTGGAGT TGGACACTCT TCCATGTTGT 
GCTGAGACAC ACAAGTGGGC GTGGTTTCGA CGCTATTGCA CCGCCTCCAG AGTGTCAGTG 
GCTCTGGACA AGAGGAGCCC TTTGCCCCGC CCTTTTCTGG ATGAGGTAGT GGCAAAGAAA 
ATCCGTGAGC TGATGGCAGA TCATGAGAAC ATGAACACGC TTCATGAGAG CCATGACTTG 
TTTAAGAGGG AGCAGGATGA GCAGTTGGTT CAGTGGATGA ACAGACGACC AGATGATTGG 
ACACTCTCTG CGGGTGGCAG TGGTACAATA TACGGCTGGG GGCACAATCA CCGGGGTCAG 
TTAGGTGGCA TTGAAGGAGC CAAAGTAAAG GTTCCCACAC CAACAGAAGC TCTAGCCACA 
CTGCGGCCAG TTCAGCTTAT TGGAGGAGAG CAAACACTGT TTGCCGTCAC TGCTGATGGG 
AAGCTGTATG CCACAGGATA TGGTGCCGGT GGTCGACTGG GAATAGGAGG GACAGAGTCC 
GTCTCCACTC CGACCCTGCT GGAGTCCATC CAGCATGTTT TCATCAGAAA GGTGGCTGTG 
AACTCTGGAG GGAAACACTG TTTGGCTCTT TCCTCTGAAG GAGAGCTGTA CTCCTGGGGG 
GAGGCCGAAG ATGGAAAACT GGGCCATGGA AACAGAAGCC CATGTGATCG TCCACGCGTG 
ATTGAGTCTC TCAGAGGGGT GGAAGTGGTG GACATTGCTG CCGGTGGGGC TCACAGTGCC 
TGCATTACTG CCAGTGGAGA GCTCTACACA TGGGGCAAAG GTCGCTATGG GCGCCTGGGT 
CATGGAGACA GCGAAGACCA ACTTAAACCA AAACTGGTGG AGGCTCTTCA GGGTCACCGG 
GTGATAGATG TGGCATGTGG CAGTGGAGAT GCACAGACCC TCTGTCTGAC TGATGATGAC 
ATGGTATGGT CGTGGGGCGA TGGAGACTAT GGCAAATTGG GCCGAGGAGG TAGTGATGGA 
TGTAAAGTGC CGATGAAGAT CGATTCATTA ACCGGATTGG GTGTTGTGAA AGTGGAATGT 
GGCTCTCAGT TTTCTGTGGC CCTTACCAAG TCTGGAGCAG TTTACACATG GGGGAAGGGT 
GACTATCACA GACTGGGACA CGGCTCAGAT GATCATGTTC GCAGACCCCG GCAGGTCCAG 
GGTTTACAAG GCAAAAAGGT CATCGCAATA GCAACTGGTT CCCTTCACTG CGTTTGCTGC 
ACGGAGGACG GGGAAGTGTA CACATGGGGT GACAATGATG AGGGTCAGCT TGGGGACGGT 
ACGACCAATG CTATTCAGAG ACCCAGGCTG GTGGCAGCGC TGCAGGGCAA GAAGATTAAT 
CGTGTGGCCT GTGGTTCAGC ACATACACTT GCCTGGTCCA CTAGTAAACC AACCAATGCT 
GGAAAACTCC CTGCTCAGGT TCCAATGGAG TACAACCACC TGCAAGAAAT CGCCATCATC 
GGCCTGAGGA ATCGGTTACT GCTACTGCAC CACATCTCTG AGCTCTTCTG CCCCTGCATC 
CCAATGTTTG ACTTAGAAGG ACGCCTGGGT GAAACTGGAC ATGGTGTGTC TGTTGGCTTT 
GACACGCTCC GTGGAATCCT CATATCACAG GGCAAGGAGG CAGCATTCCG CAAAGTGGTG 
CAGGCTACCA TGGTCAGAGA CAGGCAGCAT GGGCCAGTGG TTGAGCTGAA CAGGATACAG 
GTCAAGCGTT CACGTAGTAA AGGAGGTCTG GCAGGGCCGG ATGGCACCAA GTCAGTATTT 
GGGCAGATGT GTGCTAAGAT GAGTTCTTTC AGTCCAGACA GTCTGCTGCT GCCACACCGC 
GTCTGGAAGG TCAAGTTTGT GGGTGAGTCG GTGGATGACT GTGGTGGCGG ATACAGTGAA 
TCTATCGCAG AAATGTGCGA GGAGCTTCAG AATGGCCTCA CTCCTCTGCT CATCGTCACT 
CCTAATGGCA GAGACGAGTC AGGAGCGAAC AGAGATTGCT TTCTGCTCAA CCCAGCTGCC 
AAATCCCCTC TGCACATCAG CATGTTCCGC TTCCTAGGGG TGCTCCTGGG CATAGCGATC 
CGAACAGGAA GTCCTCTTAG TCTGTATCTG GCCGAACCTG TGTGGAAACA GCTGGCAGGC 
ATGAACCTGA CCATTGCTGA TCTCAGTGAG GTGGACAAGG ACTTCATCCC CGGTCTGATG 
TATATTAGAG ATAACGAGGC GTCTGCGGAG GAGTTTGAGG CGATGCCTCT TCCCTTCACT 
GTGCCCAGTG CCTGCGGACA AGAAGTCCAG CTCAGCTCCA AACATTCCCA CATCAGCCTT 
GAAAACCGCA GTGAATATGT GCGGCTAGCA ATAAATTACA GGCTGCATGA GTTTGATGAG 
CAGGTGTCTG CTGTAAGAGA AGGTATGGCG CGTGTGGTTC CGGTACCGCT CCTCTCTCTA 
TTTACCGGCT ATGAGCTGGA GACCATGGTG TGCGGAAGTC CGGACATCCC TCTGCATCTG 
CTCAAATCAG TGGCCACATA TAAAGGAGTA GAGCCCACTG CACCGCTCAT CCAGTGGTTC 
TGGGAGGTGA TGGAGTCTTT CTCTAACACA GAACGCTCGC TCTTCCTCAG GTTCGTCTGG 
GGCCGCACGC GTCTTCCCAG GACCATCGCA GACTTCCGTG GACGAGACTT TGTAGTGCAG 
GTGCTGGATA AATATAACCC TCCCGACCAC TTCCTCCCCG AGTCCTACAC CTGCTTCTTC 
CTGCTCAAGC TGCCTCGCTA CTCCTGCAAG CTAGTCTTGG AGGAGAAACT CAAATACGCC 
ATTCACTTCT GCAAGTCCAT CGACACTGAT GACTACGCAC GGATCGCTCT GAGCGGAGAA 
CCGGCCGCAG ACGACAGCAG TGATGACTCG GACAATGAAG ACGCAGACTC ATTCGCTTCT 
GACTCCACAC AGGATTACCT GACAGGCCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC2 ( XM_021471809.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021327484.1 Danio rerio HECT and RLD domain containing E3 ubiquitin protein ligase 2 (herc2), transcript variant X4, mRNA.

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CloneID	ODa144735
Clone ID Related Accession (Same CDS sequence)	XM_021471809.1
Accession Version	XM_021471809.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase HERC2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394658.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAGTCC ACAAACTGAA AATGATTGTA GATCCAGCTG ACAGCAGCTA CATGCCTTCA 
TTGGTGGTGG TATCAGGTGG AACCTCACTC AACAATTTGA TCGAGTTGAA GACCATCAAC 
ATCAATCCCA CTGACACTGT CGTCTCTCTA CTCAATGACT GCACAGAGTA TCACAGGTAC 
ATTGAGGTTG CTATAAAGCA GTGTCGAAGT TCAGGTATTG ACTGTAAGAT TCATGGCTTG 
AGTGCTGTGG GACGTGTCCG TGCCGAGGAT GAAGATCTTG CAACTGTACC TTTCCTGGCA 
TCTGACAACG AGGAAGAGGA AGATGAGAAG ACCGCGACTG GAAGTATGTC TCGAAAGAGA 
TTGTCAGGCC TCGAGTCTGC AGCCACTATC AGAACCAAAG TCTTTGTCTG GGGTCTTAAT 
GATAAGGACC AACTTGGAGG ACTTAAGGGG TCCAAGATCA AAGTCCCTTC ATTCTCAGAG 
ACTCTGTCTG CTCTGAATGT AGTGCAGGTG GCTGGTGGCT CTAAAAGTCT TTTTGCTGTG 
ACTGCTGAAG GTAAAGTATA TGCCTGTGGA GAGGCCACCA ATGGAAGACT TGGTTTAGGC 
CTCTCCAGTG GAACCATCCC AATTCCCAGA CAGATATCAG CTCTCAGCAA CTATGTGGTC 
AAGAAGGTTG CTGTGCATTC AGGTGGACGG CATGCCATGG CACTTACCGT GGATGGGAAA 
GTATTCTCTT GGGGTGAAGG AGACGATGGC AAGCTTGGAC ACTTCAGCCG AATGAACTGT 
GATAAGCCAA GGCTGATCGA GGCATTGAAA ACCAAACGCA TTCGAGATAT TGCGTGTGGC 
AGCTCTCACA GTGCCGCCAT CACCTCCAGT GGAGAGCTGT ACAGCTGGGG TCTGGGGGAA 
TATGGCCGTC TGGGCCATGG GGACAACACC ACCCAACTCA GACCCAAACT GGTCAAGGTG 
CTTTTAGGCC ATCGCGTTGT CCAGGTAGCT TGTGGAAGTA GAGATGCCCA AACTCTTGCA 
CTGACAGATG AAGGAATGGT GTTCTCATGG GGAGATGGAG ACTTTGGGAA ACTGGGCCGT 
GGTGGCAGCG AGGGATGTAA CATTCCTCAA AACATTGAGC GTCTGAATGG ACAGGGGGTC 
TGTCAAATTG AATGTGGAGC TCAATTCTCC CTCGCCCTCA CCAAATCTGG AGTTGTCTGG 
ACCTGGGGTA AAGGGGATTA CTTCAGACTG GGTCACGGCA CAGATGTTCA TGTCCGCAAA 
CCCCAGATGG TTGAAGGACT TAGGGGGAAG AAGATTGTTC ATGTGGCCGT TGGTGCTCTG 
CACTGCCTGG CTGTCACCGA GACTGGACAG GTGTTCGCGT GGGGGGATAA TGACCATGGG 
CAGCAGGGGA ATGGCACAAC CACAGTCAAC AGGAAGCCCA CATTGGTGCA GGGTCTAGAG 
GGTCAGAAAA TTACTCGTGT GGCCTGCGGT TCTTCTCATA GTGTTGCCTG GACCACAGTA 
GATGTCACTA CACCCTCCGT CCATGAGCCT GTGCTCTTCC AGACTGCTAG AGACTCTCTG 
GGAGCCTCTT ACCTTGGTGT GCCATCAGAT AGTGACCCGT CTTTACTGAG CAATAAGCTA 
AATGGCGTAA ACAATGTGAA GCCCAACAGG CCATCTCTGG CAAAGATCCT TCTCTCTCTG 
GATGGTAATC TGGCCAAACA GCAGGCCCTA TCCCATGTGC TGTCTTCCCT GCAGATCATG 
TATGCCAGAG ATGCAGTGGT GGGTGCATTA ATGCCATCCT GTATGATGCC TCTGGAGTGT 
CCCTCCAGCT CTCCGGTGCC CCCATCAGAC TGTTCATCAG CTTCCAGCCC CTGTGAGGCT 
GAGGGTCCTG CCCTGCCCTC AGAGAGTGAG GAGAGGGTCA GTCCGCACCC CTGGATGGAC 
AGCAAACGAG GAGAGGTAAC CACGTCTGAG GATGCCACTA CCCCATCTTC AGCAGTGACC 
CCTTCTACTG CCTCTGCCTC ATCTCGACCC TTCATACCAG TCACAGATGA TCCTGGTGCT 
GCCAGCATCA TTGCTGAGAC CATGACCAAA ACCAAAGAGG ACTCTGAGAA CCAGAGTAAA 
GCCGTGGGTC CAGAGCCACA GTATCTGGAT GAGTTCACCA GTCTCCTGAT CCCTGATGAC 
ACGCGTGTGA TGGTGGACCT GTTAAAACTA GCTGTATCTC TGCGCTCTGG TGATAAAGGA 
AAGGAAGTGC TGTCTGCTGT TCTGTCTGGC ATGGGTACAG CCTGCCCACA GGTGGCGGAT 
ATGCTTCTGG AGCTGTGTGT GACTGAGTTG GAGGACGTGG CGACTGACTG TCAGAGTGGA 
CGGCTTTCCT CTCAACCTGT GGTGGTGGAA AGCAGCCATC CATATATTGA CGATACATCC 
ACCAGCGGAA CTGTCAAAAT ACCAGGTGCT GAGGGTTTGC GGGTTGAGTT TGATCGCCAG 
TGCTCAACAG AAAGGAGACA TGACCCTCTT ACCATCATGG ATGGAGCCAA TAGAATTGTG 
TCTGTTCGAT CAGGTCGTGA GTGGTCTGAT TGGTCCAGTG AATTGAGAAT TCCAGGAGAC 
GAGCTCAAAT GGAAGTTCAC CAGTGACGGC TCTGTGAACG GCTGGGGCTG GCGCTTCACA 
GTCTATCCCA TCATGCCTGC GGCTGGTCCT AAAGATCTTC TGTCAGACCG CTGCATTTTG 
TCTTGCCCTT CAATGGATCT AGTAACATGT CTGCTAGACT TTCGTCTGAA CTTTGCCTCA 
AACCGCAGCA TTGTGCCCAG ATTAGCAGCC TCACTTGCTG CTTGTGCGCA GCTCAGCGCC 
TTGGCGGCTG GCCATCGTAT GTGGGCTCTG CAGAGGTTAC GCAAGCTCTT AACTACTGAA 
TACGGCCAAT CCATTAACAT CAACCGCCTC TTAGGAGACA CCGAAGGAGA GGCTCGCTCT 
ATGAGTTTTA CAGGAAGTGC ATTAGCTGCT CTGGTGAAAG GACTTCCTGA GGCATTACAG 
AGACAGTATG AGTACGAGGA GCCCATAGTT AGGGGTGGCA AACAGTTACT CCACAGCCCG 
TTCTTCAAGG TGTTGGTGGC GCTGGCGTGT GATCTGGAGT TGGACACTCT TCCATGTTGT 
GCTGAGACAC ACAAGTGGGC GTGGTTTCGA CGCTATTGCA CCGCCTCCAG AGTGTCAGTG 
GCTCTGGACA AGAGGAGCCC TTTGCCCCGC CCTTTTCTGG ATGAGGTGGC AAAGAAAATC 
CGTGAGCTGA TGGCAGATCA TGAGAACATG AACACGCTTC ATGAGAGCCA TGACTTGTTT 
AAGAGGGAGC AGGATGAGCA GTTGGTTCAG TGGATGAACA GACGACCAGA TGATTGGACA 
CTCTCTGCGG GTGGCAGTGG TACAATATAC GGCTGGGGGC ACAATCACCG GGGTCAGTTA 
GGTGGCATTG AAGGAGCCAA AGTAAAGGTT CCCACACCAA CAGAAGCTCT AGCCACACTG 
CGGCCAGTTC AGCTTATTGG AGGAGAGCAA ACACTGTTTG CCGTCACTGC TGATGGGAAG 
CTGTATGCCA CAGGATATGG TGCCGGTGGT CGACTGGGAA TAGGAGGGAC AGAGTCCGTC 
TCCACTCCGA CCCTGCTGGA GTCCATCCAG CATGTTTTCA TCAGAAAGGT GGCTGTGAAC 
TCTGGAGGGA AACACTGTTT GGCTCTTTCC TCTGAAGGAG AGCTGTACTC CTGGGGGGAG 
GCCGAAGATG GAAAACTGGG CCATGGAAAC AGAAGCCCAT GTGATCGTCC ACGCGTGATT 
GAGTCTCTCA GAGGGGTGGA AGTGGTGGAC ATTGCTGCCG GTGGGGCTCA CAGTGCCTGC 
ATTACTGCCA GTGGAGAGCT CTACACATGG GGCAAAGGTC GCTATGGGCG CCTGGGTCAT 
GGAGACAGCG AAGACCAACT TAAACCAAAA CTGGTGGAGG CTCTTCAGGG TCACCGGGTG 
ATAGATGTGG CATGTGGCAG TGGAGATGCA CAGACCCTCT GTCTGACTGA TGATGACATG 
GTATGGTCGT GGGGCGATGG AGACTATGGC AAATTGGGCC GAGGAGGTAG TGATGGATGT 
AAAGTGCCGA TGAAGATCGA TTCATTAACC GGATTGGGTG TTGTGAAAGT GGAATGTGGC 
TCTCAGTTTT CTGTGGCCCT TACCAAGTCT GGAGCAGTTT ACACATGGGG GAAGGGTGAC 
TATCACAGAC TGGGACACGG CTCAGATGAT CATGTTCGCA GACCCCGGCA GGTCCAGGGT 
TTACAAGGCA AAAAGGTCAT CGCAATAGCA ACTGGTTCCC TTCACTGCGT TTGCTGCACG 
GAGGACGGGG AAGTGTACAC ATGGGGTGAC AATGATGAGG GTCAGCTTGG GGACGGTACG 
ACCAATGCTA TTCAGAGACC CAGGCTGGTG GCAGCGCTGC AGGGCAAGAA GATTAATCGT 
GTGGCCTGTG GTTCAGCACA TACACTTGCC TGGTCCACTA GTAAACCAAC CAATGCTGGA 
AAACTCCCTG CTCAGGTTCC AATGGAGTAC AACCACCTGC AAGAAATCGC CATCATCGGC 
CTGAGGAATC GGTTACTGCT ACTGCACCAC ATCTCTGAGC TCTTCTGCCC CTGCATCCCA 
ATGTTTGACT TAGAAGGACG CCTGGGTGAA ACTGGACATG GTGTGTCTGT TGGCTTTGAC 
ACGCTCCGTG GAATCCTCAT ATCACAGGGC AAGGAGGCAG CATTCCGCAA AGTGGTGCAG 
GCTACCATGG TCAGAGACAG GCAGCATGGG CCAGTGGTTG AGCTGAACAG GATACAGGTC 
AAGCGTTCAC GTAGTAAAGG AGGTCTGGCA GGGCCGGATG GCACCAAGTC AGTATTTGGG 
CAGATGTGTG CTAAGATGAG TTCTTTCAGT CCAGACAGTC TGCTGCTGCC ACACCGCGTC 
TGGAAGGTCA AGTTTGTGGG TGAGTCGGTG GATGACTGTG GTGGCGGATA CAGTGAATCT 
ATCGCAGAAA TGTGCGAGGA GCTTCAGAAT GGCCTCACTC CTCTGCTCAT CGTCACTCCT 
AATGGCAGAG ACGAGTCAGG AGCGAACAGA GATTGCTTTC TGCTCAACCC AGCTGCCAAA 
TCCCCTCTGC ACATCAGCAT GTTCCGCTTC CTAGGGGTGC TCCTGGGCAT AGCGATCCGA 
ACAGGAAGTC CTCTTAGTCT GTATCTGGCC GAACCTGTGT GGAAACAGCT GGCAGGCATG 
AACCTGACCA TTGCTGATCT CAGTGAGGTG GACAAGGACT TCATCCCCGG TCTGATGTAT 
ATTAGAGATA ACGAGGCGTC TGCGGAGGAG TTTGAGGCGA TGCCTCTTCC CTTCACTGTG 
CCCAGTGCCT GCGGACAAGA AGTCCAGCTC AGCTCCAAAC ATTCCCACAT CAGCCTTGAA 
AACCGCAGTG AATATGTGCG GCTAGCAATA AATTACAGGC TGCATGAGTT TGATGAGCAG 
GTGTCTGCTG TAAGAGAAGG TATGGCGCGT GTGGTTCCGG TACCGCTCCT CTCTCTATTT 
ACCGGCTATG AGCTGGAGAC CATGGTGTGC GGAAGTCCGG ACATCCCTCT GCATCTGCTC 
AAATCAGTGG CCACATATAA AGGAGTAGAG CCCACTGCAC CGCTCATCCA GTGGTTCTGG 
GAGGTGATGG AGTCTTTCTC TAACACAGAA CGCTCGCTCT TCCTCAGGTT CGTCTGGGGC 
CGCACGCGTC TTCCCAGGAC CATCGCAGAC TTCCGTGGAC GAGACTTTGT AGTGCAGGTG 
CTGGATAAAT ATAACCCTCC CGACCACTTC CTCCCCGAGT CCTACACCTG CTTCTTCCTG 
CTCAAGCTGC CTCGCTACTC CTGCAAGCTA GTCTTGGAGG AGAAACTCAA ATACGCCATT 
CACTTCTGCA AGTCCATCGA CACTGATGAC TACGCACGGA TCGCTCTGAG CGGAGAACCG 
GCCGCAGACG ACAGCAGTGA TGACTCGGAC AATGAAGACG CAGACTCATT CGCTTCTGAC 
TCCACACAGG ATTACCTGAC AGGCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327484.1
CDS	27..6056
Translation	MLVHKLKMIVDPADSSYMPSLVVVSGGTSLNNLIELKTININPTDTVVSLLNDCTEYHRYIEVAIKQCRSSGIDCKIHGLSAVGRVRAEDEDLATVPFLASDNEEEEDEKTATGSMSRKRLSGLESAATIRTKVFVWGLNDKDQLGGLKGSKIKVPSFSETLSALNVVQVAGGSKSLFAVTAEGKVYACGEATNGRLGLGLSSGTIPIPRQISALSNYVVKKVAVHSGGRHAMALTVDGKVFSWGEGDDGKLGHFSRMNCDKPRLIEALKTKRIRDIACGSSHSAAITSSGELYSWGLGEYGRLGHGDNTTQLRPKLVKVLLGHRVVQVACGSRDAQTLALTDEGMVFSWGDGDFGKLGRGGSEGCNIPQNIERLNGQGVCQIECGAQFSLALTKSGVVWTWGKGDYFRLGHGTDVHVRKPQMVEGLRGKKIVHVAVGALHCLAVTETGQVFAWGDNDHGQQGNGTTTVNRKPTLVQGLEGQKITRVACGSSHSVAWTTVDVTTPSVHEPVLFQTARDSLGASYLGVPSDSDPSLLSNKLNGVNNVKPNRPSLAKILLSLDGNLAKQQALSHVLSSLQIMYARDAVVGALMPSCMMPLECPSSSPVPPSDCSSASSPCEAEGPALPSESEERVSPHPWMDSKRGEVTTSEDATTPSSAVTPSTASASSRPFIPVTDDPGAASIIAETMTKTKEDSENQSKAVGPEPQYLDEFTSLLIPDDTRVMVDLLKLAVSLRSGDKGKEVLSAVLSGMGTACPQVADMLLELCVTELEDVATDCQSGRLSSQPVVVESSHPYIDDTSTSGTVKIPGAEGLRVEFDRQCSTERRHDPLTIMDGANRIVSVRSGREWSDWSSELRIPGDELKWKFTSDGSVNGWGWRFTVYPIMPAAGPKDLLSDRCILSCPSMDLVTCLLDFRLNFASNRSIVPRLAASLAACAQLSALAAGHRMWALQRLRKLLTTEYGQSININRLLGDTEGEARSMSFTGSALAALVKGLPEALQRQYEYEEPIVRGGKQLLHSPFFKVLVALACDLELDTLPCCAETHKWAWFRRYCTASRVSVALDKRSPLPRPFLDEVAKKIRELMADHENMNTLHESHDLFKREQDEQLVQWMNRRPDDWTLSAGGSGTIYGWGHNHRGQLGGIEGAKVKVPTPTEALATLRPVQLIGGEQTLFAVTADGKLYATGYGAGGRLGIGGTESVSTPTLLESIQHVFIRKVAVNSGGKHCLALSSEGELYSWGEAEDGKLGHGNRSPCDRPRVIESLRGVEVVDIAAGGAHSACITASGELYTWGKGRYGRLGHGDSEDQLKPKLVEALQGHRVIDVACGSGDAQTLCLTDDDMVWSWGDGDYGKLGRGGSDGCKVPMKIDSLTGLGVVKVECGSQFSVALTKSGAVYTWGKGDYHRLGHGSDDHVRRPRQVQGLQGKKVIAIATGSLHCVCCTEDGEVYTWGDNDEGQLGDGTTNAIQRPRLVAALQGKKINRVACGSAHTLAWSTSKPTNAGKLPAQVPMEYNHLQEIAIIGLRNRLLLLHHISELFCPCIPMFDLEGRLGETGHGVSVGFDTLRGILISQGKEAAFRKVVQATMVRDRQHGPVVELNRIQVKRSRSKGGLAGPDGTKSVFGQMCAKMSSFSPDSLLLPHRVWKVKFVGESVDDCGGGYSESIAEMCEELQNGLTPLLIVTPNGRDESGANRDCFLLNPAAKSPLHISMFRFLGVLLGIAIRTGSPLSLYLAEPVWKQLAGMNLTIADLSEVDKDFIPGLMYIRDNEASAEEFEAMPLPFTVPSACGQEVQLSSKHSHISLENRSEYVRLAINYRLHEFDEQVSAVREGMARVVPVPLLSLFTGYELETMVCGSPDIPLHLLKSVATYKGVEPTAPLIQWFWEVMESFSNTERSLFLRFVWGRTRLPRTIADFRGRDFVVQVLDKYNPPDHFLPESYTCFFLLKLPRYSCKLVLEEKLKYAIHFCKSIDTDDYARIALSGEPAADDSSDDSDNEDADSFASDSTQDYLTGH

Target ORF information:

RefSeq Version	XM_021471809.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio HECT and RLD domain containing E3 ubiquitin protein ligase 2 (herc2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471809.1

ORF Insert Sequence:

ATGTTAGTCC ACAAACTGAA AATGATTGTA GATCCAGCTG ACAGCAGCTA CATGCCTTCA 
TTGGTGGTGG TATCAGGTGG AACCTCACTC AACAATTTGA TCGAGTTGAA GACCATCAAC 
ATCAATCCCA CTGACACTGT CGTCTCTCTA CTCAATGACT GCACAGAGTA TCACAGGTAC 
ATTGAGGTTG CTATAAAGCA GTGTCGAAGT TCAGGTATTG ACTGTAAGAT TCATGGCTTG 
AGTGCTGTGG GACGTGTCCG TGCCGAGGAT GAAGATCTTG CAACTGTACC TTTCCTGGCA 
TCTGACAACG AGGAAGAGGA AGATGAGAAG ACCGCGACTG GAAGTATGTC TCGAAAGAGA 
TTGTCAGGCC TCGAGTCTGC AGCCACTATC AGAACCAAAG TCTTTGTCTG GGGTCTTAAT 
GATAAGGACC AACTTGGAGG ACTTAAGGGG TCCAAGATCA AAGTCCCTTC ATTCTCAGAG 
ACTCTGTCTG CTCTGAATGT AGTGCAGGTG GCTGGTGGCT CTAAAAGTCT TTTTGCTGTG 
ACTGCTGAAG GTAAAGTATA TGCCTGTGGA GAGGCCACCA ATGGAAGACT TGGTTTAGGC 
CTCTCCAGTG GAACCATCCC AATTCCCAGA CAGATATCAG CTCTCAGCAA CTATGTGGTC 
AAGAAGGTTG CTGTGCATTC AGGTGGACGG CATGCCATGG CACTTACCGT GGATGGGAAA 
GTATTCTCTT GGGGTGAAGG AGACGATGGC AAGCTTGGAC ACTTCAGCCG AATGAACTGT 
GATAAGCCAA GGCTGATCGA GGCATTGAAA ACCAAACGCA TTCGAGATAT TGCGTGTGGC 
AGCTCTCACA GTGCCGCCAT CACCTCCAGT GGAGAGCTGT ACAGCTGGGG TCTGGGGGAA 
TATGGCCGTC TGGGCCATGG GGACAACACC ACCCAACTCA GACCCAAACT GGTCAAGGTG 
CTTTTAGGCC ATCGCGTTGT CCAGGTAGCT TGTGGAAGTA GAGATGCCCA AACTCTTGCA 
CTGACAGATG AAGGAATGGT GTTCTCATGG GGAGATGGAG ACTTTGGGAA ACTGGGCCGT 
GGTGGCAGCG AGGGATGTAA CATTCCTCAA AACATTGAGC GTCTGAATGG ACAGGGGGTC 
TGTCAAATTG AATGTGGAGC TCAATTCTCC CTCGCCCTCA CCAAATCTGG AGTTGTCTGG 
ACCTGGGGTA AAGGGGATTA CTTCAGACTG GGTCACGGCA CAGATGTTCA TGTCCGCAAA 
CCCCAGATGG TTGAAGGACT TAGGGGGAAG AAGATTGTTC ATGTGGCCGT TGGTGCTCTG 
CACTGCCTGG CTGTCACCGA GACTGGACAG GTGTTCGCGT GGGGGGATAA TGACCATGGG 
CAGCAGGGGA ATGGCACAAC CACAGTCAAC AGGAAGCCCA CATTGGTGCA GGGTCTAGAG 
GGTCAGAAAA TTACTCGTGT GGCCTGCGGT TCTTCTCATA GTGTTGCCTG GACCACAGTA 
GATGTCACTA CACCCTCCGT CCATGAGCCT GTGCTCTTCC AGACTGCTAG AGACTCTCTG 
GGAGCCTCTT ACCTTGGTGT GCCATCAGAT AGTGACCCGT CTTTACTGAG CAATAAGCTA 
AATGGCGTAA ACAATGTGAA GCCCAACAGG CCATCTCTGG CAAAGATCCT TCTCTCTCTG 
GATGGTAATC TGGCCAAACA GCAGGCCCTA TCCCATGTGC TGTCTTCCCT GCAGATCATG 
TATGCCAGAG ATGCAGTGGT GGGTGCATTA ATGCCATCCT GTATGATGCC TCTGGAGTGT 
CCCTCCAGCT CTCCGGTGCC CCCATCAGAC TGTTCATCAG CTTCCAGCCC CTGTGAGGCT 
GAGGGTCCTG CCCTGCCCTC AGAGAGTGAG GAGAGGGTCA GTCCGCACCC CTGGATGGAC 
AGCAAACGAG GAGAGGTAAC CACGTCTGAG GATGCCACTA CCCCATCTTC AGCAGTGACC 
CCTTCTACTG CCTCTGCCTC ATCTCGACCC TTCATACCAG TCACAGATGA TCCTGGTGCT 
GCCAGCATCA TTGCTGAGAC CATGACCAAA ACCAAAGAGG ACTCTGAGAA CCAGAGTAAA 
GCCGTGGGTC CAGAGCCACA GTATCTGGAT GAGTTCACCA GTCTCCTGAT CCCTGATGAC 
ACGCGTGTGA TGGTGGACCT GTTAAAACTA GCTGTATCTC TGCGCTCTGG TGATAAAGGA 
AAGGAAGTGC TGTCTGCTGT TCTGTCTGGC ATGGGTACAG CCTGCCCACA GGTGGCGGAT 
ATGCTTCTGG AGCTGTGTGT GACTGAGTTG GAGGACGTGG CGACTGACTG TCAGAGTGGA 
CGGCTTTCCT CTCAACCTGT GGTGGTGGAA AGCAGCCATC CATATATTGA CGATACATCC 
ACCAGCGGAA CTGTCAAAAT ACCAGGTGCT GAGGGTTTGC GGGTTGAGTT TGATCGCCAG 
TGCTCAACAG AAAGGAGACA TGACCCTCTT ACCATCATGG ATGGAGCCAA TAGAATTGTG 
TCTGTTCGAT CAGGTCGTGA GTGGTCTGAT TGGTCCAGTG AATTGAGAAT TCCAGGAGAC 
GAGCTCAAAT GGAAGTTCAC CAGTGACGGC TCTGTGAACG GCTGGGGCTG GCGCTTCACA 
GTCTATCCCA TCATGCCTGC GGCTGGTCCT AAAGATCTTC TGTCAGACCG CTGCATTTTG 
TCTTGCCCTT CAATGGATCT AGTAACATGT CTGCTAGACT TTCGTCTGAA CTTTGCCTCA 
AACCGCAGCA TTGTGCCCAG ATTAGCAGCC TCACTTGCTG CTTGTGCGCA GCTCAGCGCC 
TTGGCGGCTG GCCATCGTAT GTGGGCTCTG CAGAGGTTAC GCAAGCTCTT AACTACTGAA 
TACGGCCAAT CCATTAACAT CAACCGCCTC TTAGGAGACA CCGAAGGAGA GGCTCGCTCT 
ATGAGTTTTA CAGGAAGTGC ATTAGCTGCT CTGGTGAAAG GACTTCCTGA GGCATTACAG 
AGACAGTATG AGTACGAGGA GCCCATAGTT AGGGGTGGCA AACAGTTACT CCACAGCCCG 
TTCTTCAAGG TGTTGGTGGC GCTGGCGTGT GATCTGGAGT TGGACACTCT TCCATGTTGT 
GCTGAGACAC ACAAGTGGGC GTGGTTTCGA CGCTATTGCA CCGCCTCCAG AGTGTCAGTG 
GCTCTGGACA AGAGGAGCCC TTTGCCCCGC CCTTTTCTGG ATGAGGTGGC AAAGAAAATC 
CGTGAGCTGA TGGCAGATCA TGAGAACATG AACACGCTTC ATGAGAGCCA TGACTTGTTT 
AAGAGGGAGC AGGATGAGCA GTTGGTTCAG TGGATGAACA GACGACCAGA TGATTGGACA 
CTCTCTGCGG GTGGCAGTGG TACAATATAC GGCTGGGGGC ACAATCACCG GGGTCAGTTA 
GGTGGCATTG AAGGAGCCAA AGTAAAGGTT CCCACACCAA CAGAAGCTCT AGCCACACTG 
CGGCCAGTTC AGCTTATTGG AGGAGAGCAA ACACTGTTTG CCGTCACTGC TGATGGGAAG 
CTGTATGCCA CAGGATATGG TGCCGGTGGT CGACTGGGAA TAGGAGGGAC AGAGTCCGTC 
TCCACTCCGA CCCTGCTGGA GTCCATCCAG CATGTTTTCA TCAGAAAGGT GGCTGTGAAC 
TCTGGAGGGA AACACTGTTT GGCTCTTTCC TCTGAAGGAG AGCTGTACTC CTGGGGGGAG 
GCCGAAGATG GAAAACTGGG CCATGGAAAC AGAAGCCCAT GTGATCGTCC ACGCGTGATT 
GAGTCTCTCA GAGGGGTGGA AGTGGTGGAC ATTGCTGCCG GTGGGGCTCA CAGTGCCTGC 
ATTACTGCCA GTGGAGAGCT CTACACATGG GGCAAAGGTC GCTATGGGCG CCTGGGTCAT 
GGAGACAGCG AAGACCAACT TAAACCAAAA CTGGTGGAGG CTCTTCAGGG TCACCGGGTG 
ATAGATGTGG CATGTGGCAG TGGAGATGCA CAGACCCTCT GTCTGACTGA TGATGACATG 
GTATGGTCGT GGGGCGATGG AGACTATGGC AAATTGGGCC GAGGAGGTAG TGATGGATGT 
AAAGTGCCGA TGAAGATCGA TTCATTAACC GGATTGGGTG TTGTGAAAGT GGAATGTGGC 
TCTCAGTTTT CTGTGGCCCT TACCAAGTCT GGAGCAGTTT ACACATGGGG GAAGGGTGAC 
TATCACAGAC TGGGACACGG CTCAGATGAT CATGTTCGCA GACCCCGGCA GGTCCAGGGT 
TTACAAGGCA AAAAGGTCAT CGCAATAGCA ACTGGTTCCC TTCACTGCGT TTGCTGCACG 
GAGGACGGGG AAGTGTACAC ATGGGGTGAC AATGATGAGG GTCAGCTTGG GGACGGTACG 
ACCAATGCTA TTCAGAGACC CAGGCTGGTG GCAGCGCTGC AGGGCAAGAA GATTAATCGT 
GTGGCCTGTG GTTCAGCACA TACACTTGCC TGGTCCACTA GTAAACCAAC CAATGCTGGA 
AAACTCCCTG CTCAGGTTCC AATGGAGTAC AACCACCTGC AAGAAATCGC CATCATCGGC 
CTGAGGAATC GGTTACTGCT ACTGCACCAC ATCTCTGAGC TCTTCTGCCC CTGCATCCCA 
ATGTTTGACT TAGAAGGACG CCTGGGTGAA ACTGGACATG GTGTGTCTGT TGGCTTTGAC 
ACGCTCCGTG GAATCCTCAT ATCACAGGGC AAGGAGGCAG CATTCCGCAA AGTGGTGCAG 
GCTACCATGG TCAGAGACAG GCAGCATGGG CCAGTGGTTG AGCTGAACAG GATACAGGTC 
AAGCGTTCAC GTAGTAAAGG AGGTCTGGCA GGGCCGGATG GCACCAAGTC AGTATTTGGG 
CAGATGTGTG CTAAGATGAG TTCTTTCAGT CCAGACAGTC TGCTGCTGCC ACACCGCGTC 
TGGAAGGTCA AGTTTGTGGG TGAGTCGGTG GATGACTGTG GTGGCGGATA CAGTGAATCT 
ATCGCAGAAA TGTGCGAGGA GCTTCAGAAT GGCCTCACTC CTCTGCTCAT CGTCACTCCT 
AATGGCAGAG ACGAGTCAGG AGCGAACAGA GATTGCTTTC TGCTCAACCC AGCTGCCAAA 
TCCCCTCTGC ACATCAGCAT GTTCCGCTTC CTAGGGGTGC TCCTGGGCAT AGCGATCCGA 
ACAGGAAGTC CTCTTAGTCT GTATCTGGCC GAACCTGTGT GGAAACAGCT GGCAGGCATG 
AACCTGACCA TTGCTGATCT CAGTGAGGTG GACAAGGACT TCATCCCCGG TCTGATGTAT 
ATTAGAGATA ACGAGGCGTC TGCGGAGGAG TTTGAGGCGA TGCCTCTTCC CTTCACTGTG 
CCCAGTGCCT GCGGACAAGA AGTCCAGCTC AGCTCCAAAC ATTCCCACAT CAGCCTTGAA 
AACCGCAGTG AATATGTGCG GCTAGCAATA AATTACAGGC TGCATGAGTT TGATGAGCAG 
GTGTCTGCTG TAAGAGAAGG TATGGCGCGT GTGGTTCCGG TACCGCTCCT CTCTCTATTT 
ACCGGCTATG AGCTGGAGAC CATGGTGTGC GGAAGTCCGG ACATCCCTCT GCATCTGCTC 
AAATCAGTGG CCACATATAA AGGAGTAGAG CCCACTGCAC CGCTCATCCA GTGGTTCTGG 
GAGGTGATGG AGTCTTTCTC TAACACAGAA CGCTCGCTCT TCCTCAGGTT CGTCTGGGGC 
CGCACGCGTC TTCCCAGGAC CATCGCAGAC TTCCGTGGAC GAGACTTTGT AGTGCAGGTG 
CTGGATAAAT ATAACCCTCC CGACCACTTC CTCCCCGAGT CCTACACCTG CTTCTTCCTG 
CTCAAGCTGC CTCGCTACTC CTGCAAGCTA GTCTTGGAGG AGAAACTCAA ATACGCCATT 
CACTTCTGCA AGTCCATCGA CACTGATGAC TACGCACGGA TCGCTCTGAG CGGAGAACCG 
GCCGCAGACG ACAGCAGTGA TGACTCGGAC AATGAAGACG CAGACTCATT CGCTTCTGAC 
TCCACACAGG ATTACCTGAC AGGCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC2 ( XM_021477195.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021332870.1 Danio rerio HECT and RLD domain containing E3 ubiquitin protein ligase 2 (herc2), transcript variant X1, mRNA.

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CloneID	ODa143098
Clone ID Related Accession (Same CDS sequence)	XM_021477195.1
Accession Version	XM_021477195.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 14496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase HERC2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTCTC CATCATTTTC CCTGAATGCC CAGCTGCGCT TCCATGATAA ATGGCTGAAG 
ATCGACTTGC AGCGCGCCTT CTCTCATGAA GGACTGAGCG AGATGTGGAA CGAGATGGTG 
AAGGATGAAG AGATCGTCTT TGACAGAGAA GAGTCTGCCC ATCCAGAGGA CTGTGCTGAT 
TGCACCCAAA AGAAAGATGA CACCAATGAT AAAGAAAAGA AGGAAGAGGA GGAGGCAGCA 
GCAACGGCGG CTTCATCTTT CCATCACTCC ATCATTGAAA TGTGGGACTG GGGGAGGCAG 
CCAGAAACCA GCGAGCTGCG GGACTGTCTG ATGGCTTTGG TAGACGACCA ACAGAAGCTG 
TCTTCTCAGA CGGCTAAGAG CACACTCTCA GCCCTGCGTC TAAGCCAGAG ACTGGTGATC 
CTGGAGCGCT ACCTCATCTC GCTCACTCAC AGCATGATGG AGGAGAAGTA CAAAGTACGG 
TGGAAGAGCC CTCCCAGTCC TCCACTCTCT GCTGTCGACA ACAAAAGCGC TCGGCCAGTT 
AGTAAAGGGG TGGAAGGTTT GGCCAGGGTG GGCTCAAGGG CAGCTCTTTC CTTTGCCTTT 
GCATTTCTCC GCCGAGCCTG GAGATCAGGA GATGATGCAG ACCTGTGCAG TGAGTTACTG 
CAGGAGTCTC TGGATGCTCT GCGGGCTCTT CCAGAAGCTA CTCTGTTTGA TGAGGGCACT 
GTATCTCCTG TTTGGCTGGA GGTGGTTGAG AGGGCTACTA AATTTCTAAG TGACATCCAT 
GGAAGTGCCA GTGGAAAAGC ACCCAGTAAC ATTCCCTTGC AGGATCAGCA CTTGGCCCTT 
GCCATCCTGC TGGAGCTCGC AGTGCAACGA GGAACTCTCA GTCAGCTGCT GTCTGCAGTT 
CTGCTGCTTC TGAGGCTCTG GGACAGTGGA ACTAGAGAGA TGGATAATGA GCGCTCCACC 
CAAGGCACCA GCGCCCCGTT ACTGCCCCTC CTGCAGCGCT TCCAGAACAT CCACAGCAGT 
AAAGAGGAGC CTGTTCCTGA AGAGGAGATT GAGATCCTTT CGGCCCCTCT GAGCCCGAAT 
GAGAGTTTTC TGCGCTACCT TACACTGCCT CAAGATAATG ACCTGGCCAT TGATTTGCGG 
CAGACAGCTG TAGTCATCAT GGCACATTTG GACCGCCTGG CAGCCCCATG CAGCCCCCCG 
CAGTGCAGCT CACCCACTTC TAACAAGGGG ACACTGCAGG AGGTGATTGG TTGGGGTCTG 
CTTGGATGGA AGCCTTATGC TAATGTGAAT GGACCAATTC AGTGCAAGGC ACTATCAAAT 
CTGGGTGTCA CGCAGATTGT CTGCTCAGAG AAGGGTTTCC TCATCCTGAC CATCACTGGT 
GCTGTCTACA CACAAAACTA CAAAAGTACT ACACTAGCAC CTATGCTAGT CCATGCACTG 
ACGTCCAGGA AAATAATGAA GCTTGCAGCT CATCCTGATG GCCAGCATTA TTTGGCTCTT 
TCAGTCAATG GAGAGGTTTT CTCCTGGGGT TGTGGAGATG GAGGCAGGCT AGGACATGGA 
GACACTACGT ATTTAGAGGA GCCCACTATG ATTTCAGTAT TCTCAGGGAG GCCGGTAGGG 
AAGCAGGTTG TACATATTGC CTGTGGCAGC ACATACAGCG CAGCCATCAC AGCAGATGGA 
GAGCTTCACA CATGGGGCCG AGGAAACTAC GGCAGACTGG GCCATGGCTC CAGTGAGGAC 
CAGACCACTC CCATGCTAGT AACTGCACTG AAGGGGCTGA AGGTGATCGA TGTGGCCTGT 
GGAAGCGGAG ATGCTCAGAC GCTTGCTGTG ACTGAGAATG GTCAGGTATG GTCATGGGGT 
GATGGAGATT ATGGGAAACT GGGCCGAGGG GGCAGTGATG GATGCAAAAC ACCAAAACTA 
GTGGAGAAGC TCCAGGATCT GGATATTGTG AAGGTGTGCT GTGGGAGCCA GTTCTCCGTA 
GCGCTCACTA AAGATGGACA AGTTTACACC TGGGGCAAAG GAGACAATCA ACGACTTGGA 
CATGGCACTG ATGAACATGT CCGCTATCCA AAACTACTTG ACAGTCTGCA AGGCAAGAGG 
GTGGTGGACA TAGCGGTTGG CTCCACACAC TGCCTGGCCC TTACAGAAGA AGGAGAGGTG 
CACAGCTGGG GCAGTAATGA CAAACTACAG CACTTTGACA CACTTTTCTC CAATAAGAAG 
CAGCCCAAAG CCTTGCCAGG CCTCAACTCC AAACACATAG TAGGCATCTC CTGCGGTCCT 
GGCCAGAGTT TTTCATGGTC ATCCTGCTCT GAGTGGTCTG TGGGTCTCCG GGTACCCTTC 
GTGGTAGACG TGTGCACTAT GACCTTTGAC CAGCTGGACC TGCTTCTGCG CCAGGTTAGT 
GAGGGCATGG ACGGCAGCTC TGATTGGCCA CCACCACAGG AGAAAGAATG CATGGTCGTG 
GCAGCTCTCA ACCTGCTCAG ACTACAGCTT CATGCAGCCA TCAGTAATCA GGTGGATCCC 
GAGGAGCTGG GTCTGGGTCT GGGCAGTGTG CTGCTTAATA ACCTCAAGCA GACGGTGGTG 
ACTCTGGCCA GCAACGGCGG GGTGCTAAAC ACTGTGCAGG CTGCCGCCCA GACCGTGCTC 
CAGAGTGGCT GGTCAGTGCT ACTCCCCACT GCAGAGGAAC GAGCAAGAGC CCTGTCCTCA 
CTGCTGCCCA ATGCAGCATC TGCTAATGAG ATGAGTGTAA GTCCTGGCCG TCGCTTCATG 
ATCGACCTCT TGGTTAGTAG TCTGATGGCA GATGGAGGGT TGGAGTCTGC TTTGAATGCT 
GCCATCACGG CCGAGATCCA GGATATTGAG GCAAAGAAAG AGGCTCAGAA AGAGAAGGAA 
ATCGATGAGC AGGAAGCCAG CGCCTCTTCT TTGCATCGCT GTCGCACCAC ACTGGACAAA 
GACCTGATCA ACACCGGCAT CTATGAGTCT GCCAACAAAC AGAGCCTCCC ATTGGTCCAG 
CTTGTCCAAC AGCTGCTCAG GAACATTGCT TCACAGACTA TAGCCCGTCT TAAGGATGTG 
GCCCGACGCA TCTCAAATTG TCAAGACACC GAGCAGGTCA GCAAGGAGCG CTCAGCCTCT 
CTGGACCTGC TGCTGCGCTT TCAGAGGCTT TTGGTCTGCA AATTGTATGT CGGAGTCAAT 
GGCACTGTGG GTGTGGGAGG ATACACTTTG AGTCACTCAG AAATTCTGGG AGTTGGCTCT 
CTCCTGAAGA AGTATATGGC TTTGCTTTGC ACACACATCG GTGATATCCT TCCTGTGGCC 
ACCAGCATTG CCCTCAACAG CCATCGTCAC TTTGCAGAGG TGGCTCGTGT CCTTGAGGGA 
GATCTTACAG GTGTTCTGCT ACCAGAGCTC ATTGTGTCCA TTGTGCTGCT GCTGACCCAT 
GATGCTGGAC TAATGCAAGA GACTGGATCC ATTCCTCTGC TCGCTGCTCT TCTGGAGCAC 
TTGGACCGTT TTAACTATCT TGCACCTGGT ACAGAAAGAG ATGACAATGA GGATCTTGCC 
TGGCCTGGAC TCATGGGTTT CTTTTTCAAT GGACCGAGCT CAAAGAACAA CGAGGAGGTG 
TCACTTATCC GCAAAGCAGA CCTGGAGAAT CACAACAAAG ATGGCGGCTT TTGGACTGTA 
ATTGAAGGGA AGGTCTATGA CATAAAGGAC TTTCAAGCTC AGACTCTAAC TGGCAACAGC 
ATCCTTGCCC AGTTTGCAGG AGAAGACCCT GTGGTGGCAT TAGAGGCTGC CCTCCAATTT 
GAAGACACCA GAGAGTCCAT GCAAGCTTTC TGTGTGGGAC AGTATATGGA GCCCAACCAA 
GAAATGGTGA CAACGCCTGA CCTGAGCAGC CTGTCCTCTC CTCTTATAGA TGCTGAGAGG 
AACCTTGGTC TGCTTCTGGG GCTACATGCC TCATATCTGG CTATGAGCAC CCCTCTCTCC 
CGCATAGAGT TAGAATGTGC CAAATGGCTC CAGTCATCTA TTTTCTTCGG TGGACTTCAG 
ACCAGTCAGA TCCACTATAA TTATAACGAG GAGAAGGATG AGGACCACTG CAGCTCTCCA 
GGGACCACCA CACCAGACAA AGCCAAACTG TACTGCCGCA GAATCACCCT GAGTGATCAC 
GCCCAGCCCT TCCTGCAGGC CATCGCTGAC AACACCACCC AGGATCACAC TGTGAAGGAC 
TTTTTATGCC AAATCGAGCG GTGCTGTAAA CAGTACCATC TCATCACTCC CATCACTTTT 
CCACCTGAAC ATCCTGTGGA GGAAGTGGGT CGCCTGCTGC TCTGCTGCCT TCTGAAGCAT 
CAGGATCTTG GTCATGTCGC CCTCTCCCTA GTTGATCAAT GTGCCTTGGG TATGGACCAG 
GGGAAACAGA GGTCCCTCCC GAAATCTGTG ATTGATGTGT GTCGAATGGT GTATCAGGCC 
AAGTGTTCGT TGATAAAAAC ACACCAGGAA CAGGGTCGTT CTTATAAAGA GGTGTGCGCT 
CCTGTTATTG AGAGGTTGCG TTTCCTGTTT AATGAGCTAC GACCTGCCGT CAGTAATGAC 
TTGTCCATTG TGTCCAAACT CAAACTGCTG AGCTCTCAGC CTCGCTGGAA GAGGATCACA 
CAGAAACTGA TCAGAGACCG CCGCAAAAAG AGAGTGCCTA AAAAGTCGGA GTCAGCTGAT 
CTGGAGGAGT GCAAGATGGA AGGGACCAAT GAGGAACCAC CTGCTCCTCT AAGCCCCGCT 
CCTGTTGACA AGAAGCAAAC AACTGTCAAA TCTGCAAAGC AGGATAAGTG GCAGCCTCTG 
CTAAACACCA TCGGCAGTGT TCAGAAGTAC CGCTGGTTAA GGCACAGCGT TCAGGGCTCC 
TTCTCTCAGT CCAGCCTCAT GAGCACCATC GTTGAGTTTT CTTTGAAAGA GGAGCCGCTG 
GATGTGGAGA AGATCAGAAA GTGCCTCCTT AAACAGCTGG AGAGAGCTGA GGTCCGCCTG 
GAGGGTATTG ACACTATGTT GAAGCTGGCA TCTAAAAACT TCCTCCTGCC CTCTGTTCAG 
TATGCCATGC TTTGTGGCTG GCAGAGAGTC ATACCTGAAG GGACCAATAT TGGAGAGCCA 
TTGGCTGACT GTCTAAAGGA TGTAGATCTC ATTCCTCCAT TCAACCGCAT GCTACTAGAG 
GTGACGTTTG GGAAGCTCTA TGCCTGGTCC ATACAGAACG TCCGCAACAT CTTGTTGGAG 
GCTAGTGTCA AGTTCAAGGA ACTGGGTGTG CAACCAGTGC CTCTCCAGAC AATCACTAAT 
GAGAACCCAG CAGGCCCCAG TCTGGGTACC ATCCCTCAGG CACGCTTCCT GCTGGCTATG 
CTCCATATGC TCACTCTCAA ACACGGAGCC AACAGCCTCA GCCTCCTGCT GAACTCTGGC 
ATCCTCGCTC TCACCCAGAG TGTCCTCCGA CTCATTGGTC CTAGCTGTGA TAGCAGTGAG 
GAGGAGCTGA CCTCTGTAGC TCATGGAGGC TCTGCCACAG TTCTGGAAGA GTCCAGAAAG 
GAGACTGCCC CGACCCCTCT GCCCGCTTCC GGCCCTGAGC TGGCAGCTAT GATGAAGATC 
AGCACAAGGG TGATGAGAGG TCCTGACTGG AAGTGGGGGG ACCAGGATGG CCCATCACCA 
GGCCTGGGTC GTGTGATTGG GGAGTTGGGA GAAGATGGTT GGATAAGAGT CCAATGGGAC 
ACCGGCAGCA CCAACTCCTA CAGGATGGGA AAAGAGGGCA AGTATGACCT TAAACTGGCT 
GAGCCGCCAC CTGCCACCCA ACCAGCCACT GAAGACTCTG ACACGGAGGA TGACACAGAA 
GGGGAGGTGA TGGAAAAGTG TGCCCACCCC ACGGCAATCA TGCTGACTAG TACAGTAAAT 
CTGTTGAAGA CACTGTCACT GTCTTCTGGG ATTCATGCAG AGGTGATGCA GGTCGATGCG 
GTCAGTACTC TGTGCGGGCT ACTCAGGATA CTGGTGGAGA GTGGAGCCAA CGACAAGACT 
GGTTGCTCCT CTCACAAGCT GGTGTCCCGA GAGCAGCACA GGAGCTGGTG CACGCTGGGA 
TTTATTCGCA GTATAGCTCT TATGCCTCAG ATGTGCAGTA CCCTGAGCTC CTCTGCCTGG 
ATCAATCTGC TTATACGCAT CGTGGAGGGT CATCAGTCCT TCACTGCAAT CACACTACAG 
AGACAGATTC TTGCCCTGCG GCTTCTACAG GCAGTATTGC CCTCCTGGGA TAAGACTGAA 
CGCTCTCAGG ATATGAAGTT CTTGGTGGAG AAACTGTTTG GGTTCCTCGG CAGCTTGCTC 
AGCACTTGTT CTTCAGATCT GCCTCTTCTT AGGGAAGGTT CAATGCGTAG GAGGAAGTCC 
CGCCCCCAGG CCTCACTGAC AGCCACTCAT AGCAGTACTT TGGCAGAGGA AATAGTGACA 
GTATTGCGCA CGCTTCACTC CCTCAGCCAA TGGAATGCCC TGATTAATGA ATACATTAAC 
TCTCAGCTCA GTGGAATCGG GGATGTCATG GCAGGCCGAC ACACTGAAGT GGCTTTGCTT 
GAAGACTATC TCCCCAATGC GGAAGCAGCA GCGGTGGGCA GTCTGATGGC TGTTTTGGCA 
GTCATCGGGG GGATTGATGG ACGTCTGCGT CTGGGTGGAC GTGTGGTCCA TGAGGAGTAT 
GGAGAAGGCA CAGTGACTCG CATCACACCT AAAGGACGAA TCACTGTTCA GTTTCATGAG 
ATGAGGAACT GCAAAGTGTG CCTGCTCAGT CAGCTCAAAC CGCTTCACAT GATTCCATTC 
AGCGTTCAGA ATCTGCCCTT CGCTGAGCCC ATGCTAATGG TGTGGGCCCA GCTAGTCAAC 
TTGGCTGGGA GCAAGCTGGA GAAACAACGC ATGAAGAAAT CTCTTAGTAG AGGACTTACA 
GCGGATCAGG TAGATGTACA TTTGTTGCGC TGTCAGCAGC TCAGGCTGTA CATCTTGAAG 
GCTGCACGAG CTCTACTCTC CCATCAGGAC AAACTCAGAC AGATCCTTTC TCAGGCGGCC 
GTGGTTGATG TCAGCCCCAA TCCTACAGAG GATCAGTGCG CGTCGTCTCC TGATGTAGGA 
GATTTGTCTC CTGAGGGTCC TCAGCCTCCT CTCATCCTCC TTCAGCAGTT GCTGTCAGCA 
TCCACTCAAC CTTCACCCAT CAAAGCCATA TTCCACCGCC AGGAGTTAGA GGCAGCGGCT 
CTGGCTGTGT GTCAGTACCT GGCGGTTGAA TCCACACACC CGTCATCGCC TCTGTTTGAG 
GACAGCAGCT CTAGCGAAGC CACTACACCA GTCACTGTGC AGCACATGCG TCCACCCAAA 
CAAAAGAAAC ACAAGACGTC ACCTGTGCCC CCTGCTCCCA TCGTCCTGCA GCTGATGGAG 
ATGGGCTTTC AAAGGAAAAA CATCGAGTTT GCTCTTAAAT CACTGTCTGG AACCACAGGT 
GCTTCTGGAG TGCCAGGTGT CGAGGCATTG GTGGGCTGGT TGTTGGACCA TCCAGATGTT 
CTCATTACAG ATCTGTCGGA TGCAGAGACT GTATCAGATG AATACTCTGA TGAAGAGGTG 
CTGGAAGAGC TTGAGGAGCC TGAGCCTGCC TTTCCTGTGC CTCCTGGGGT TGTGGTGACT 
GAGAGTCAGA CGTTTAAAAA GAGGTCGGAC TTTCAGAGCA ATGACGATTA TGCTGTTTAC 
GTGAGAGAGA ACATTCAGGT GGGCATGATG GTACGGTGTT GCAGGACATA TGAGGAAGTG 
TATGAAGGAG ATGTGGGGAA AGTGATCAAG CTTGACAGAG ACGGCCTGCA TGACCTGAAT 
GTTCAGTGTG ACTGGCAGCA GAAAGGAGGA ACTTACTGGG TCCGTTACAT CCACACTGAG 
CTGCTGGGGT TTCCCCCTCA GAGTTCTCCA TCTCATATAA AGATTGGTGA TAAAGTGCGA 
GTGAAGCCCT CGGTCACAAC ACCAAAATAC AAGTGGGGGT CAGTCACTCA TAGGAGTGTG 
GGTGTGGTCA AAGCCTTCAG TGCCAATGGG AAGGATGTCA TTGTGGACTT TCCTCAGCAG 
TCGCACTGGA CTGGACTGCT GTCAGAGATG GAGCTTGTGC CCAGCGTACA TCCTGGTGTC 
AGGTGTGACG GATGTCAGAT GTTCCCTATA AATGGCCCAA GGTTTAAGTG TCGGAGCTGT 
GATGACTTTG ACTTTTGTGA GAGCTGCTTC AAGACCCGTA AACACAATCC TAGGCATTCA 
TTTGGGAGGA TAAACGAACC AGGTCAGTCA CCCACCTTTA GTGGGCGCTC AGGAAAGCAG 
CTGAAAAGAC ATCACAGCAG TCAGCGTGGC ATGCTGATCG ACGAGTGGTC AAGAGTGGTG 
AAGAACCTCA GCGTATCGTC TTCTGTTAAC CAGGCGTCTC GACTCATCGA CTGTGGAGAG 
CAATGCTGGC AGTCCTCAGG CTCTCAGGGC AAGCACTGGA TCAGATTGGA GCTTTTCCCA 
GACATGTTAG TCCACAAACT GAAAATGATT GTAGATCCAG CTGACAGCAG CTACATGCCT 
TCATTGGTGG TGGTATCAGG TGGAACCTCA CTCAACAATT TGATCGAGTT GAAGACCATC 
AACATCAATC CCACTGACAC TGTCGTCTCT CTACTCAATG ACTGCACAGA GTATCACAGG 
TACATTGAGG TTGCTATAAA GCAGTGTCGA AGTTCAGGTA TTGACTGTAA GATTCATGGC 
TTGAGTGCTG TGGGACGTGT CCGTGCCGAG GATGAAGATC TTGCAACTGT ACCTTTCCTG 
GCATCTGACA ACGAGGAAGA GGAAGATGAG AAGACCGCGA CTGGAAGTAT GTCTCGAAAG 
AGATTGTCAG GCCTCGAGTC TGCAGCCACT ATCAGAACCA AAGTCTTTGT CTGGGGTCTT 
AATGATAAGG ACCAACTTGG AGGACTTAAG GGGTCCAAGA TCAAAGTCCC TTCATTCTCA 
GAGACTCTGT CTGCTCTGAA TGTAGTGCAG GTGGCTGGTG GCTCTAAAAG TCTTTTTGCT 
GTGACTGCTG AAGGTAAAGT ATATGCCTGT GGAGAGGCCA CCAATGGAAG ACTTGGTTTA 
GGCCTCTCCA GTGGAACCAT CCCAATTCCC AGACAGATAT CAGCTCTCAG CAACTATGTG 
GTCAAGAAGG TTGCTGTGCA TTCAGGTGGA CGGCATGCCA TGGCACTTAC CGTGGATGGG 
AAAGTATTCT CTTGGGGTGA AGGAGACGAT GGCAAGCTTG GACACTTCAG CCGAATGAAC 
TGTGATAAGC CAAGGCTGAT CGAGGCATTG AAAACCAAAC GCATTCGAGA TATTGCGTGT 
GGCAGCTCTC ACAGTGCCGC CATCACCTCC AGTGGAGAGC TGTACAGCTG GGGTCTGGGG 
GAATATGGCC GTCTGGGCCA TGGGGACAAC ACCACCCAAC TCAGACCCAA ACTGGTCAAG 
GTGCTTTTAG GCCATCGCGT TGTCCAGGTA GCTTGTGGAA GTAGAGATGC CCAAACTCTT 
GCACTGACAG ATGAAGGAAT GGTGTTCTCA TGGGGAGATG GAGACTTTGG GAAACTGGGC 
CGTGGTGGCA GCGAGGGATG TAACATTCCT CAAAACATTG AGCGTCTGAA TGGACAGGGG 
GTCTGTCAAA TTGAATGTGG AGCTCAATTC TCCCTCGCCC TCACCAAATC TGGAGTTGTC 
TGGACCTGGG GTAAAGGGGA TTACTTCAGA CTGGGTCACG GCACAGATGT TCATGTCCGC 
AAACCCCAGA TGGTTGAAGG ACTTAGGGGG AAGAAGATTG TTCATGTGGC CGTTGGTGCT 
CTGCACTGCC TGGCTGTCAC CGAGACTGGA CAGGTGTTCG CGTGGGGGGA TAATGACCAT 
GGGCAGCAGG GGAATGGCAC AACCACAGTC AACAGGAAGC CCACATTGGT GCAGGGTCTA 
GAGGGTCAGA AAATTACTCG TGTGGCCTGC GGTTCTTCTC ATAGTGTTGC CTGGACCACA 
GTAGATGTCA CTACACCCTC CGTCCATGAG CCTGTGCTCT TCCAGACTGC TAGAGACTCT 
CTGGGAGCCT CTTACCTTGG TGTGCCATCA GATAGTGACC CGTCTTTACT GAGCAATAAG 
CTAAATGGCG TAAACAATGT GAAGCCCAAC AGGCCATCTC TGGCAAAGAT CCTTCTCTCT 
CTGGATGGTA ATCTGGCCAA ACAGCAGGCC CTATCCCATG TGCTGTCTTC CCTGCAGATC 
ATGTATGCCA GAGATGCAGT GGTGGGTGCA TTAATGCCAT CCTGTATGAT GCCTCTGGAG 
TGTCCCTCCA GCTCTCCGGT GCCCCCATCA GACTGTTCAT CAGCTTCCAG CCCCTGTGAG 
GCTGAGGGTC CTGCCCTGCC CTCAGAGAGT GAGGAGAGGG TCAGTCCGCA CCCCTGGATG 
GACAGCAAAC GAGGAGAGGT AACCACGTCT GAGGATGCCA CTACCCCATC TTCAGCAGTG 
ACCCCTTCTA CTGCCTCTGC CTCATCTCGA CCCTTCATAC CAGTCACAGA TGATCCTGGT 
GCTGCCAGCA TCATTGCTGA GACCATGACC AAAACCAAAG AGGACTCTGA GAACCAGAGT 
AAAGCCGTGG GTCCAGAGCC ACAGTATCTG GATGAGTTCA CCAGTCTCCT GATCCCTGAT 
GACACGCGTG TGATGGTGGA CCTGTTAAAA CTAGCTGTAT CTCTGCGCTC TGGTGATAAA 
GGAAAGGAAG TGCTGTCTGC TGTTCTGTCT GGCATGGGTA CAGCCTGCCC ACAGGTGGCG 
GATATGCTTC TGGAGCTGTG TGTGACTGAG TTGGAGGACG TGGCGACTGA CTGTCAGAGT 
GGACGGCTTT CCTCTCAACC TGTGGTGGTG GAAAGCAGCC ATCCATATAT TGACGATACA 
TCCACCAGCG GAACTGTCAA AATACCAGGT GCTGAGGGTT TGCGGGTTGA GTTTGATCGC 
CAGTGCTCAA CAGAAAGGAG ACATGACCCT CTTACCATCA TGGATGGAGC CAATAGAATT 
GTGTCTGTTC GATCAGGTCG TGAGTGGTCT GATTGGTCCA GTGAATTGAG AATTCCAGGA 
GACGAGCTCA AATGGAAGTT CACCAGTGAC GGCTCTGTGA ACGGCTGGGG CTGGCGCTTC 
ACAGTCTATC CCATCATGCC TGCGGCTGGT CCTAAAGATC TTCTGTCAGA CCGCTGCATT 
TTGTCTTGCC CTTCAATGGA TCTAGTAACA TGTCTGCTAG ACTTTCGTCT GAACTTTGCC 
TCAAACCGCA GCATTGTGCC CAGATTAGCA GCCTCACTTG CTGCTTGTGC GCAGCTCAGC 
GCCTTGGCGG CTGGCCATCG TATGTGGGCT CTGCAGAGGT TACGCAAGCT CTTAACTACT 
GAATACGGCC AATCCATTAA CATCAACCGC CTCTTAGGAG ACACCGAAGG AGAGGCTCGC 
TCTATGAGTT TTACAGGAAG TGCATTAGCT GCTCTGGTGA AAGGACTTCC TGAGGCATTA 
CAGAGACAGT ATGAGTACGA GGAGCCCATA GTTAGGGGTG GCAAACAGTT ACTCCACAGC 
CCGTTCTTCA AGGTGTTGGT GGCGCTGGCG TGTGATCTGG AGTTGGACAC TCTTCCATGT 
TGTGCTGAGA CACACAAGTG GGCGTGGTTT CGACGCTATT GCACCGCCTC CAGAGTGTCA 
GTGGCTCTGG ACAAGAGGAG CCCTTTGCCC CGCCCTTTTC TGGATGAGGT AGTGGCAAAG 
AAAATCCGTG AGCTGATGGC AGATCATGAG AACATGAACA CGCTTCATGA GAGCCATGAC 
TTGTTTAAGA GGGAGCAGGA TGAGCAGTTG GTTCAGTGGA TGAACAGACG ACCAGATGAT 
TGGACACTCT CTGCGGGTGG CAGTGGTACA ATATACGGCT GGGGGCACAA TCACCGGGGT 
CAGTTAGGTG GCATTGAAGG AGCCAAAGTA AAGGTTCCCA CACCAACAGA AGCTCTAGCC 
ACACTGCGGC CAGTTCAGCT TATTGGAGGA GAGCAAACAC TGTTTGCCGT CACTGCTGAT 
GGGAAGCTGT ATGCCACAGG ATATGGTGCC GGTGGTCGAC TGGGAATAGG AGGGACAGAG 
TCCGTCTCCA CTCCGACCCT GCTGGAGTCC ATCCAGCATG TTTTCATCAG AAAGGTGGCT 
GTGAACTCTG GAGGGAAACA CTGTTTGGCT CTTTCCTCTG AAGGAGAGCT GTACTCCTGG 
GGGGAGGCCG AAGATGGAAA ACTGGGCCAT GGAAACAGAA GCCCATGTGA TCGTCCACGC 
GTGATTGAGT CTCTCAGAGG GGTGGAAGTG GTGGACATTG CTGCCGGTGG GGCTCACAGT 
GCCTGCATTA CTGCCAGTGG AGAGCTCTAC ACATGGGGCA AAGGTCGCTA TGGGCGCCTG 
GGTCATGGAG ACAGCGAAGA CCAACTTAAA CCAAAACTGG TGGAGGCTCT TCAGGGTCAC 
CGGGTGATAG ATGTGGCATG TGGCAGTGGA GATGCACAGA CCCTCTGTCT GACTGATGAT 
GACATGGTAT GGTCGTGGGG CGATGGAGAC TATGGCAAAT TGGGCCGAGG AGGTAGTGAT 
GGATGTAAAG TGCCGATGAA GATCGATTCA TTAACCGGAT TGGGTGTTGT GAAAGTGGAA 
TGTGGCTCTC AGTTTTCTGT GGCCCTTACC AAGTCTGGAG CAGTTTACAC ATGGGGGAAG 
GGTGACTATC ACAGACTGGG ACACGGCTCA GATGATCATG TTCGCAGACC CCGGCAGGTC 
CAGGGTTTAC AAGGCAAAAA GGTCATCGCA ATAGCAACTG GTTCCCTTCA CTGCGTTTGC 
TGCACGGAGG ACGGGGAAGT GTACACATGG GGTGACAATG ATGAGGGTCA GCTTGGGGAC 
GGTACGACCA ATGCTATTCA GAGACCCAGG CTGGTGGCAG CGCTGCAGGG CAAGAAGATT 
AATCGTGTGG CCTGTGGTTC AGCACATACA CTTGCCTGGT CCACTAGTAA ACCAACCAAT 
GCTGGAAAAC TCCCTGCTCA GGTTCCAATG GAGTACAACC ACCTGCAAGA AATCGCCATC 
ATCGGCCTGA GGAATCGGTT ACTGCTACTG CACCACATCT CTGAGCTCTT CTGCCCCTGC 
ATCCCAATGT TTGACTTAGA AGGACGCCTG GGTGAAACTG GACATGGTGT GTCTGTTGGC 
TTTGACACGC TCCGTGGAAT CCTCATATCA CAGGGCAAGG AGGCAGCATT CCGCAAAGTG 
GTGCAGGCTA CCATGGTCAG AGACAGGCAG CATGGGCCAG TGGTTGAGCT GAACAGGATA 
CAGGTCAAGC GTTCACGTAG TAAAGGAGGT CTGGCAGGGC CGGATGGCAC CAAGTCAGTA 
TTTGGGCAGA TGTGTGCTAA GATGAGTTCT TTCAGTCCAG ACAGTCTGCT GCTGCCACAC 
CGCGTCTGGA AGGTCAAGTT TGTGGGTGAG TCGGTGGATG ACTGTGGTGG CGGATACAGT 
GAATCTATCG CAGAAATGTG CGAGGAGCTT CAGAATGGCC TCACTCCTCT GCTCATCGTC 
ACTCCTAATG GCAGAGACGA GTCAGGAGCG AACAGAGATT GCTTTCTGCT CAACCCAGCT 
GCCAAATCCC CTCTGCACAT CAGCATGTTC CGCTTCCTAG GGGTGCTCCT GGGCATAGCG 
ATCCGAACAG GAAGTCCTCT TAGTCTGTAT CTGGCCGAAC CTGTGTGGAA ACAGCTGGCA 
GGCATGAACC TGACCATTGC TGATCTCAGT GAGGTGGACA AGGACTTCAT CCCCGGTCTG 
ATGTATATTA GAGATAACGA GGCGTCTGCG GAGGAGTTTG AGGCGATGCC TCTTCCCTTC 
ACTGTGCCCA GTGCCTGCGG ACAAGAAGTC CAGCTCAGCT CCAAACATTC CCACATCAGC 
CTTGAAAACC GCAGTGAATA TGTGCGGCTA GCAATAAATT ACAGGCTGCA TGAGTTTGAT 
GAGCAGGTGT CTGCTGTAAG AGAAGGTATG GCGCGTGTGG TTCCGGTACC GCTCCTCTCT 
CTATTTACCG GCTATGAGCT GGAGACCATG GTGTGCGGAA GTCCGGACAT CCCTCTGCAT 
CTGCTCAAAT CAGTGGCCAC ATATAAAGGA GTAGAGCCCA CTGCACCGCT CATCCAGTGG 
TTCTGGGAGG TGATGGAGTC TTTCTCTAAC ACAGAACGCT CGCTCTTCCT CAGGTTCGTC 
TGGGGCCGCA CGCGTCTTCC CAGGACCATC GCAGACTTCC GTGGACGAGA CTTTGTAGTG 
CAGGTGCTGG ATAAATATAA CCCTCCCGAC CACTTCCTCC CCGAGTCCTA CACCTGCTTC 
TTCCTGCTCA AGCTGCCTCG CTACTCCTGC AAGCTAGTCT TGGAGGAGAA ACTCAAATAC 
GCCATTCACT TCTGCAAGTC CATCGACACT GATGACTACG CACGGATCGC TCTGAGCGGA 
GAACCGGCCG CAGACGACAG CAGTGATGAC TCGGACAATG AAGACGCAGA CTCATTCGCT 
TCTGACTCCA CACAGGATTA CCTGACAGGC CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332870.1
CDS	149..14644
Translation	MPSPSFSLNAQLRFHDKWLKIDLQRAFSHEGLSEMWNEMVKDEEIVFDREESAHPEDCADCTQKKDDTNDKEKKEEEEAAATAASSFHHSIIEMWDWGRQPETSELRDCLMALVDDQQKLSSQTAKSTLSALRLSQRLVILERYLISLTHSMMEEKYKVRWKSPPSPPLSAVDNKSARPVSKGVEGLARVGSRAALSFAFAFLRRAWRSGDDADLCSELLQESLDALRALPEATLFDEGTVSPVWLEVVERATKFLSDIHGSASGKAPSNIPLQDQHLALAILLELAVQRGTLSQLLSAVLLLLRLWDSGTREMDNERSTQGTSAPLLPLLQRFQNIHSSKEEPVPEEEIEILSAPLSPNESFLRYLTLPQDNDLAIDLRQTAVVIMAHLDRLAAPCSPPQCSSPTSNKGTLQEVIGWGLLGWKPYANVNGPIQCKALSNLGVTQIVCSEKGFLILTITGAVYTQNYKSTTLAPMLVHALTSRKIMKLAAHPDGQHYLALSVNGEVFSWGCGDGGRLGHGDTTYLEEPTMISVFSGRPVGKQVVHIACGSTYSAAITADGELHTWGRGNYGRLGHGSSEDQTTPMLVTALKGLKVIDVACGSGDAQTLAVTENGQVWSWGDGDYGKLGRGGSDGCKTPKLVEKLQDLDIVKVCCGSQFSVALTKDGQVYTWGKGDNQRLGHGTDEHVRYPKLLDSLQGKRVVDIAVGSTHCLALTEEGEVHSWGSNDKLQHFDTLFSNKKQPKALPGLNSKHIVGISCGPGQSFSWSSCSEWSVGLRVPFVVDVCTMTFDQLDLLLRQVSEGMDGSSDWPPPQEKECMVVAALNLLRLQLHAAISNQVDPEELGLGLGSVLLNNLKQTVVTLASNGGVLNTVQAAAQTVLQSGWSVLLPTAEERARALSSLLPNAASANEMSVSPGRRFMIDLLVSSLMADGGLESALNAAITAEIQDIEAKKEAQKEKEIDEQEASASSLHRCRTTLDKDLINTGIYESANKQSLPLVQLVQQLLRNIASQTIARLKDVARRISNCQDTEQVSKERSASLDLLLRFQRLLVCKLYVGVNGTVGVGGYTLSHSEILGVGSLLKKYMALLCTHIGDILPVATSIALNSHRHFAEVARVLEGDLTGVLLPELIVSIVLLLTHDAGLMQETGSIPLLAALLEHLDRFNYLAPGTERDDNEDLAWPGLMGFFFNGPSSKNNEEVSLIRKADLENHNKDGGFWTVIEGKVYDIKDFQAQTLTGNSILAQFAGEDPVVALEAALQFEDTRESMQAFCVGQYMEPNQEMVTTPDLSSLSSPLIDAERNLGLLLGLHASYLAMSTPLSRIELECAKWLQSSIFFGGLQTSQIHYNYNEEKDEDHCSSPGTTTPDKAKLYCRRITLSDHAQPFLQAIADNTTQDHTVKDFLCQIERCCKQYHLITPITFPPEHPVEEVGRLLLCCLLKHQDLGHVALSLVDQCALGMDQGKQRSLPKSVIDVCRMVYQAKCSLIKTHQEQGRSYKEVCAPVIERLRFLFNELRPAVSNDLSIVSKLKLLSSQPRWKRITQKLIRDRRKKRVPKKSESADLEECKMEGTNEEPPAPLSPAPVDKKQTTVKSAKQDKWQPLLNTIGSVQKYRWLRHSVQGSFSQSSLMSTIVEFSLKEEPLDVEKIRKCLLKQLERAEVRLEGIDTMLKLASKNFLLPSVQYAMLCGWQRVIPEGTNIGEPLADCLKDVDLIPPFNRMLLEVTFGKLYAWSIQNVRNILLEASVKFKELGVQPVPLQTITNENPAGPSLGTIPQARFLLAMLHMLTLKHGANSLSLLLNSGILALTQSVLRLIGPSCDSSEEELTSVAHGGSATVLEESRKETAPTPLPASGPELAAMMKISTRVMRGPDWKWGDQDGPSPGLGRVIGELGEDGWIRVQWDTGSTNSYRMGKEGKYDLKLAEPPPATQPATEDSDTEDDTEGEVMEKCAHPTAIMLTSTVNLLKTLSLSSGIHAEVMQVDAVSTLCGLLRILVESGANDKTGCSSHKLVSREQHRSWCTLGFIRSIALMPQMCSTLSSSAWINLLIRIVEGHQSFTAITLQRQILALRLLQAVLPSWDKTERSQDMKFLVEKLFGFLGSLLSTCSSDLPLLREGSMRRRKSRPQASLTATHSSTLAEEIVTVLRTLHSLSQWNALINEYINSQLSGIGDVMAGRHTEVALLEDYLPNAEAAAVGSLMAVLAVIGGIDGRLRLGGRVVHEEYGEGTVTRITPKGRITVQFHEMRNCKVCLLSQLKPLHMIPFSVQNLPFAEPMLMVWAQLVNLAGSKLEKQRMKKSLSRGLTADQVDVHLLRCQQLRLYILKAARALLSHQDKLRQILSQAAVVDVSPNPTEDQCASSPDVGDLSPEGPQPPLILLQQLLSASTQPSPIKAIFHRQELEAAALAVCQYLAVESTHPSSPLFEDSSSSEATTPVTVQHMRPPKQKKHKTSPVPPAPIVLQLMEMGFQRKNIEFALKSLSGTTGASGVPGVEALVGWLLDHPDVLITDLSDAETVSDEYSDEEVLEELEEPEPAFPVPPGVVVTESQTFKKRSDFQSNDDYAVYVRENIQVGMMVRCCRTYEEVYEGDVGKVIKLDRDGLHDLNVQCDWQQKGGTYWVRYIHTELLGFPPQSSPSHIKIGDKVRVKPSVTTPKYKWGSVTHRSVGVVKAFSANGKDVIVDFPQQSHWTGLLSEMELVPSVHPGVRCDGCQMFPINGPRFKCRSCDDFDFCESCFKTRKHNPRHSFGRINEPGQSPTFSGRSGKQLKRHHSSQRGMLIDEWSRVVKNLSVSSSVNQASRLIDCGEQCWQSSGSQGKHWIRLELFPDMLVHKLKMIVDPADSSYMPSLVVVSGGTSLNNLIELKTININPTDTVVSLLNDCTEYHRYIEVAIKQCRSSGIDCKIHGLSAVGRVRAEDEDLATVPFLASDNEEEEDEKTATGSMSRKRLSGLESAATIRTKVFVWGLNDKDQLGGLKGSKIKVPSFSETLSALNVVQVAGGSKSLFAVTAEGKVYACGEATNGRLGLGLSSGTIPIPRQISALSNYVVKKVAVHSGGRHAMALTVDGKVFSWGEGDDGKLGHFSRMNCDKPRLIEALKTKRIRDIACGSSHSAAITSSGELYSWGLGEYGRLGHGDNTTQLRPKLVKVLLGHRVVQVACGSRDAQTLALTDEGMVFSWGDGDFGKLGRGGSEGCNIPQNIERLNGQGVCQIECGAQFSLALTKSGVVWTWGKGDYFRLGHGTDVHVRKPQMVEGLRGKKIVHVAVGALHCLAVTETGQVFAWGDNDHGQQGNGTTTVNRKPTLVQGLEGQKITRVACGSSHSVAWTTVDVTTPSVHEPVLFQTARDSLGASYLGVPSDSDPSLLSNKLNGVNNVKPNRPSLAKILLSLDGNLAKQQALSHVLSSLQIMYARDAVVGALMPSCMMPLECPSSSPVPPSDCSSASSPCEAEGPALPSESEERVSPHPWMDSKRGEVTTSEDATTPSSAVTPSTASASSRPFIPVTDDPGAASIIAETMTKTKEDSENQSKAVGPEPQYLDEFTSLLIPDDTRVMVDLLKLAVSLRSGDKGKEVLSAVLSGMGTACPQVADMLLELCVTELEDVATDCQSGRLSSQPVVVESSHPYIDDTSTSGTVKIPGAEGLRVEFDRQCSTERRHDPLTIMDGANRIVSVRSGREWSDWSSELRIPGDELKWKFTSDGSVNGWGWRFTVYPIMPAAGPKDLLSDRCILSCPSMDLVTCLLDFRLNFASNRSIVPRLAASLAACAQLSALAAGHRMWALQRLRKLLTTEYGQSININRLLGDTEGEARSMSFTGSALAALVKGLPEALQRQYEYEEPIVRGGKQLLHSPFFKVLVALACDLELDTLPCCAETHKWAWFRRYCTASRVSVALDKRSPLPRPFLDEVVAKKIRELMADHENMNTLHESHDLFKREQDEQLVQWMNRRPDDWTLSAGGSGTIYGWGHNHRGQLGGIEGAKVKVPTPTEALATLRPVQLIGGEQTLFAVTADGKLYATGYGAGGRLGIGGTESVSTPTLLESIQHVFIRKVAVNSGGKHCLALSSEGELYSWGEAEDGKLGHGNRSPCDRPRVIESLRGVEVVDIAAGGAHSACITASGELYTWGKGRYGRLGHGDSEDQLKPKLVEALQGHRVIDVACGSGDAQTLCLTDDDMVWSWGDGDYGKLGRGGSDGCKVPMKIDSLTGLGVVKVECGSQFSVALTKSGAVYTWGKGDYHRLGHGSDDHVRRPRQVQGLQGKKVIAIATGSLHCVCCTEDGEVYTWGDNDEGQLGDGTTNAIQRPRLVAALQGKKINRVACGSAHTLAWSTSKPTNAGKLPAQVPMEYNHLQEIAIIGLRNRLLLLHHISELFCPCIPMFDLEGRLGETGHGVSVGFDTLRGILISQGKEAAFRKVVQATMVRDRQHGPVVELNRIQVKRSRSKGGLAGPDGTKSVFGQMCAKMSSFSPDSLLLPHRVWKVKFVGESVDDCGGGYSESIAEMCEELQNGLTPLLIVTPNGRDESGANRDCFLLNPAAKSPLHISMFRFLGVLLGIAIRTGSPLSLYLAEPVWKQLAGMNLTIADLSEVDKDFIPGLMYIRDNEASAEEFEAMPLPFTVPSACGQEVQLSSKHSHISLENRSEYVRLAINYRLHEFDEQVSAVREGMARVVPVPLLSLFTGYELETMVCGSPDIPLHLLKSVATYKGVEPTAPLIQWFWEVMESFSNTERSLFLRFVWGRTRLPRTIADFRGRDFVVQVLDKYNPPDHFLPESYTCFFLLKLPRYSCKLVLEEKLKYAIHFCKSIDTDDYARIALSGEPAADDSSDDSDNEDADSFASDSTQDYLTGH

Target ORF information:

RefSeq Version	XM_021477195.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio HECT and RLD domain containing E3 ubiquitin protein ligase 2 (herc2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477195.1

ORF Insert Sequence:

ATGCCTTCTC CATCATTTTC CCTGAATGCC CAGCTGCGCT TCCATGATAA ATGGCTGAAG 
ATCGACTTGC AGCGCGCCTT CTCTCATGAA GGACTGAGCG AGATGTGGAA CGAGATGGTG 
AAGGATGAAG AGATCGTCTT TGACAGAGAA GAGTCTGCCC ATCCAGAGGA CTGTGCTGAT 
TGCACCCAAA AGAAAGATGA CACCAATGAT AAAGAAAAGA AGGAAGAGGA GGAGGCAGCA 
GCAACGGCGG CTTCATCTTT CCATCACTCC ATCATTGAAA TGTGGGACTG GGGGAGGCAG 
CCAGAAACCA GCGAGCTGCG GGACTGTCTG ATGGCTTTGG TAGACGACCA ACAGAAGCTG 
TCTTCTCAGA CGGCTAAGAG CACACTCTCA GCCCTGCGTC TAAGCCAGAG ACTGGTGATC 
CTGGAGCGCT ACCTCATCTC GCTCACTCAC AGCATGATGG AGGAGAAGTA CAAAGTACGG 
TGGAAGAGCC CTCCCAGTCC TCCACTCTCT GCTGTCGACA ACAAAAGCGC TCGGCCAGTT 
AGTAAAGGGG TGGAAGGTTT GGCCAGGGTG GGCTCAAGGG CAGCTCTTTC CTTTGCCTTT 
GCATTTCTCC GCCGAGCCTG GAGATCAGGA GATGATGCAG ACCTGTGCAG TGAGTTACTG 
CAGGAGTCTC TGGATGCTCT GCGGGCTCTT CCAGAAGCTA CTCTGTTTGA TGAGGGCACT 
GTATCTCCTG TTTGGCTGGA GGTGGTTGAG AGGGCTACTA AATTTCTAAG TGACATCCAT 
GGAAGTGCCA GTGGAAAAGC ACCCAGTAAC ATTCCCTTGC AGGATCAGCA CTTGGCCCTT 
GCCATCCTGC TGGAGCTCGC AGTGCAACGA GGAACTCTCA GTCAGCTGCT GTCTGCAGTT 
CTGCTGCTTC TGAGGCTCTG GGACAGTGGA ACTAGAGAGA TGGATAATGA GCGCTCCACC 
CAAGGCACCA GCGCCCCGTT ACTGCCCCTC CTGCAGCGCT TCCAGAACAT CCACAGCAGT 
AAAGAGGAGC CTGTTCCTGA AGAGGAGATT GAGATCCTTT CGGCCCCTCT GAGCCCGAAT 
GAGAGTTTTC TGCGCTACCT TACACTGCCT CAAGATAATG ACCTGGCCAT TGATTTGCGG 
CAGACAGCTG TAGTCATCAT GGCACATTTG GACCGCCTGG CAGCCCCATG CAGCCCCCCG 
CAGTGCAGCT CACCCACTTC TAACAAGGGG ACACTGCAGG AGGTGATTGG TTGGGGTCTG 
CTTGGATGGA AGCCTTATGC TAATGTGAAT GGACCAATTC AGTGCAAGGC ACTATCAAAT 
CTGGGTGTCA CGCAGATTGT CTGCTCAGAG AAGGGTTTCC TCATCCTGAC CATCACTGGT 
GCTGTCTACA CACAAAACTA CAAAAGTACT ACACTAGCAC CTATGCTAGT CCATGCACTG 
ACGTCCAGGA AAATAATGAA GCTTGCAGCT CATCCTGATG GCCAGCATTA TTTGGCTCTT 
TCAGTCAATG GAGAGGTTTT CTCCTGGGGT TGTGGAGATG GAGGCAGGCT AGGACATGGA 
GACACTACGT ATTTAGAGGA GCCCACTATG ATTTCAGTAT TCTCAGGGAG GCCGGTAGGG 
AAGCAGGTTG TACATATTGC CTGTGGCAGC ACATACAGCG CAGCCATCAC AGCAGATGGA 
GAGCTTCACA CATGGGGCCG AGGAAACTAC GGCAGACTGG GCCATGGCTC CAGTGAGGAC 
CAGACCACTC CCATGCTAGT AACTGCACTG AAGGGGCTGA AGGTGATCGA TGTGGCCTGT 
GGAAGCGGAG ATGCTCAGAC GCTTGCTGTG ACTGAGAATG GTCAGGTATG GTCATGGGGT 
GATGGAGATT ATGGGAAACT GGGCCGAGGG GGCAGTGATG GATGCAAAAC ACCAAAACTA 
GTGGAGAAGC TCCAGGATCT GGATATTGTG AAGGTGTGCT GTGGGAGCCA GTTCTCCGTA 
GCGCTCACTA AAGATGGACA AGTTTACACC TGGGGCAAAG GAGACAATCA ACGACTTGGA 
CATGGCACTG ATGAACATGT CCGCTATCCA AAACTACTTG ACAGTCTGCA AGGCAAGAGG 
GTGGTGGACA TAGCGGTTGG CTCCACACAC TGCCTGGCCC TTACAGAAGA AGGAGAGGTG 
CACAGCTGGG GCAGTAATGA CAAACTACAG CACTTTGACA CACTTTTCTC CAATAAGAAG 
CAGCCCAAAG CCTTGCCAGG CCTCAACTCC AAACACATAG TAGGCATCTC CTGCGGTCCT 
GGCCAGAGTT TTTCATGGTC ATCCTGCTCT GAGTGGTCTG TGGGTCTCCG GGTACCCTTC 
GTGGTAGACG TGTGCACTAT GACCTTTGAC CAGCTGGACC TGCTTCTGCG CCAGGTTAGT 
GAGGGCATGG ACGGCAGCTC TGATTGGCCA CCACCACAGG AGAAAGAATG CATGGTCGTG 
GCAGCTCTCA ACCTGCTCAG ACTACAGCTT CATGCAGCCA TCAGTAATCA GGTGGATCCC 
GAGGAGCTGG GTCTGGGTCT GGGCAGTGTG CTGCTTAATA ACCTCAAGCA GACGGTGGTG 
ACTCTGGCCA GCAACGGCGG GGTGCTAAAC ACTGTGCAGG CTGCCGCCCA GACCGTGCTC 
CAGAGTGGCT GGTCAGTGCT ACTCCCCACT GCAGAGGAAC GAGCAAGAGC CCTGTCCTCA 
CTGCTGCCCA ATGCAGCATC TGCTAATGAG ATGAGTGTAA GTCCTGGCCG TCGCTTCATG 
ATCGACCTCT TGGTTAGTAG TCTGATGGCA GATGGAGGGT TGGAGTCTGC TTTGAATGCT 
GCCATCACGG CCGAGATCCA GGATATTGAG GCAAAGAAAG AGGCTCAGAA AGAGAAGGAA 
ATCGATGAGC AGGAAGCCAG CGCCTCTTCT TTGCATCGCT GTCGCACCAC ACTGGACAAA 
GACCTGATCA ACACCGGCAT CTATGAGTCT GCCAACAAAC AGAGCCTCCC ATTGGTCCAG 
CTTGTCCAAC AGCTGCTCAG GAACATTGCT TCACAGACTA TAGCCCGTCT TAAGGATGTG 
GCCCGACGCA TCTCAAATTG TCAAGACACC GAGCAGGTCA GCAAGGAGCG CTCAGCCTCT 
CTGGACCTGC TGCTGCGCTT TCAGAGGCTT TTGGTCTGCA AATTGTATGT CGGAGTCAAT 
GGCACTGTGG GTGTGGGAGG ATACACTTTG AGTCACTCAG AAATTCTGGG AGTTGGCTCT 
CTCCTGAAGA AGTATATGGC TTTGCTTTGC ACACACATCG GTGATATCCT TCCTGTGGCC 
ACCAGCATTG CCCTCAACAG CCATCGTCAC TTTGCAGAGG TGGCTCGTGT CCTTGAGGGA 
GATCTTACAG GTGTTCTGCT ACCAGAGCTC ATTGTGTCCA TTGTGCTGCT GCTGACCCAT 
GATGCTGGAC TAATGCAAGA GACTGGATCC ATTCCTCTGC TCGCTGCTCT TCTGGAGCAC 
TTGGACCGTT TTAACTATCT TGCACCTGGT ACAGAAAGAG ATGACAATGA GGATCTTGCC 
TGGCCTGGAC TCATGGGTTT CTTTTTCAAT GGACCGAGCT CAAAGAACAA CGAGGAGGTG 
TCACTTATCC GCAAAGCAGA CCTGGAGAAT CACAACAAAG ATGGCGGCTT TTGGACTGTA 
ATTGAAGGGA AGGTCTATGA CATAAAGGAC TTTCAAGCTC AGACTCTAAC TGGCAACAGC 
ATCCTTGCCC AGTTTGCAGG AGAAGACCCT GTGGTGGCAT TAGAGGCTGC CCTCCAATTT 
GAAGACACCA GAGAGTCCAT GCAAGCTTTC TGTGTGGGAC AGTATATGGA GCCCAACCAA 
GAAATGGTGA CAACGCCTGA CCTGAGCAGC CTGTCCTCTC CTCTTATAGA TGCTGAGAGG 
AACCTTGGTC TGCTTCTGGG GCTACATGCC TCATATCTGG CTATGAGCAC CCCTCTCTCC 
CGCATAGAGT TAGAATGTGC CAAATGGCTC CAGTCATCTA TTTTCTTCGG TGGACTTCAG 
ACCAGTCAGA TCCACTATAA TTATAACGAG GAGAAGGATG AGGACCACTG CAGCTCTCCA 
GGGACCACCA CACCAGACAA AGCCAAACTG TACTGCCGCA GAATCACCCT GAGTGATCAC 
GCCCAGCCCT TCCTGCAGGC CATCGCTGAC AACACCACCC AGGATCACAC TGTGAAGGAC 
TTTTTATGCC AAATCGAGCG GTGCTGTAAA CAGTACCATC TCATCACTCC CATCACTTTT 
CCACCTGAAC ATCCTGTGGA GGAAGTGGGT CGCCTGCTGC TCTGCTGCCT TCTGAAGCAT 
CAGGATCTTG GTCATGTCGC CCTCTCCCTA GTTGATCAAT GTGCCTTGGG TATGGACCAG 
GGGAAACAGA GGTCCCTCCC GAAATCTGTG ATTGATGTGT GTCGAATGGT GTATCAGGCC 
AAGTGTTCGT TGATAAAAAC ACACCAGGAA CAGGGTCGTT CTTATAAAGA GGTGTGCGCT 
CCTGTTATTG AGAGGTTGCG TTTCCTGTTT AATGAGCTAC GACCTGCCGT CAGTAATGAC 
TTGTCCATTG TGTCCAAACT CAAACTGCTG AGCTCTCAGC CTCGCTGGAA GAGGATCACA 
CAGAAACTGA TCAGAGACCG CCGCAAAAAG AGAGTGCCTA AAAAGTCGGA GTCAGCTGAT 
CTGGAGGAGT GCAAGATGGA AGGGACCAAT GAGGAACCAC CTGCTCCTCT AAGCCCCGCT 
CCTGTTGACA AGAAGCAAAC AACTGTCAAA TCTGCAAAGC AGGATAAGTG GCAGCCTCTG 
CTAAACACCA TCGGCAGTGT TCAGAAGTAC CGCTGGTTAA GGCACAGCGT TCAGGGCTCC 
TTCTCTCAGT CCAGCCTCAT GAGCACCATC GTTGAGTTTT CTTTGAAAGA GGAGCCGCTG 
GATGTGGAGA AGATCAGAAA GTGCCTCCTT AAACAGCTGG AGAGAGCTGA GGTCCGCCTG 
GAGGGTATTG ACACTATGTT GAAGCTGGCA TCTAAAAACT TCCTCCTGCC CTCTGTTCAG 
TATGCCATGC TTTGTGGCTG GCAGAGAGTC ATACCTGAAG GGACCAATAT TGGAGAGCCA 
TTGGCTGACT GTCTAAAGGA TGTAGATCTC ATTCCTCCAT TCAACCGCAT GCTACTAGAG 
GTGACGTTTG GGAAGCTCTA TGCCTGGTCC ATACAGAACG TCCGCAACAT CTTGTTGGAG 
GCTAGTGTCA AGTTCAAGGA ACTGGGTGTG CAACCAGTGC CTCTCCAGAC AATCACTAAT 
GAGAACCCAG CAGGCCCCAG TCTGGGTACC ATCCCTCAGG CACGCTTCCT GCTGGCTATG 
CTCCATATGC TCACTCTCAA ACACGGAGCC AACAGCCTCA GCCTCCTGCT GAACTCTGGC 
ATCCTCGCTC TCACCCAGAG TGTCCTCCGA CTCATTGGTC CTAGCTGTGA TAGCAGTGAG 
GAGGAGCTGA CCTCTGTAGC TCATGGAGGC TCTGCCACAG TTCTGGAAGA GTCCAGAAAG 
GAGACTGCCC CGACCCCTCT GCCCGCTTCC GGCCCTGAGC TGGCAGCTAT GATGAAGATC 
AGCACAAGGG TGATGAGAGG TCCTGACTGG AAGTGGGGGG ACCAGGATGG CCCATCACCA 
GGCCTGGGTC GTGTGATTGG GGAGTTGGGA GAAGATGGTT GGATAAGAGT CCAATGGGAC 
ACCGGCAGCA CCAACTCCTA CAGGATGGGA AAAGAGGGCA AGTATGACCT TAAACTGGCT 
GAGCCGCCAC CTGCCACCCA ACCAGCCACT GAAGACTCTG ACACGGAGGA TGACACAGAA 
GGGGAGGTGA TGGAAAAGTG TGCCCACCCC ACGGCAATCA TGCTGACTAG TACAGTAAAT 
CTGTTGAAGA CACTGTCACT GTCTTCTGGG ATTCATGCAG AGGTGATGCA GGTCGATGCG 
GTCAGTACTC TGTGCGGGCT ACTCAGGATA CTGGTGGAGA GTGGAGCCAA CGACAAGACT 
GGTTGCTCCT CTCACAAGCT GGTGTCCCGA GAGCAGCACA GGAGCTGGTG CACGCTGGGA 
TTTATTCGCA GTATAGCTCT TATGCCTCAG ATGTGCAGTA CCCTGAGCTC CTCTGCCTGG 
ATCAATCTGC TTATACGCAT CGTGGAGGGT CATCAGTCCT TCACTGCAAT CACACTACAG 
AGACAGATTC TTGCCCTGCG GCTTCTACAG GCAGTATTGC CCTCCTGGGA TAAGACTGAA 
CGCTCTCAGG ATATGAAGTT CTTGGTGGAG AAACTGTTTG GGTTCCTCGG CAGCTTGCTC 
AGCACTTGTT CTTCAGATCT GCCTCTTCTT AGGGAAGGTT CAATGCGTAG GAGGAAGTCC 
CGCCCCCAGG CCTCACTGAC AGCCACTCAT AGCAGTACTT TGGCAGAGGA AATAGTGACA 
GTATTGCGCA CGCTTCACTC CCTCAGCCAA TGGAATGCCC TGATTAATGA ATACATTAAC 
TCTCAGCTCA GTGGAATCGG GGATGTCATG GCAGGCCGAC ACACTGAAGT GGCTTTGCTT 
GAAGACTATC TCCCCAATGC GGAAGCAGCA GCGGTGGGCA GTCTGATGGC TGTTTTGGCA 
GTCATCGGGG GGATTGATGG ACGTCTGCGT CTGGGTGGAC GTGTGGTCCA TGAGGAGTAT 
GGAGAAGGCA CAGTGACTCG CATCACACCT AAAGGACGAA TCACTGTTCA GTTTCATGAG 
ATGAGGAACT GCAAAGTGTG CCTGCTCAGT CAGCTCAAAC CGCTTCACAT GATTCCATTC 
AGCGTTCAGA ATCTGCCCTT CGCTGAGCCC ATGCTAATGG TGTGGGCCCA GCTAGTCAAC 
TTGGCTGGGA GCAAGCTGGA GAAACAACGC ATGAAGAAAT CTCTTAGTAG AGGACTTACA 
GCGGATCAGG TAGATGTACA TTTGTTGCGC TGTCAGCAGC TCAGGCTGTA CATCTTGAAG 
GCTGCACGAG CTCTACTCTC CCATCAGGAC AAACTCAGAC AGATCCTTTC TCAGGCGGCC 
GTGGTTGATG TCAGCCCCAA TCCTACAGAG GATCAGTGCG CGTCGTCTCC TGATGTAGGA 
GATTTGTCTC CTGAGGGTCC TCAGCCTCCT CTCATCCTCC TTCAGCAGTT GCTGTCAGCA 
TCCACTCAAC CTTCACCCAT CAAAGCCATA TTCCACCGCC AGGAGTTAGA GGCAGCGGCT 
CTGGCTGTGT GTCAGTACCT GGCGGTTGAA TCCACACACC CGTCATCGCC TCTGTTTGAG 
GACAGCAGCT CTAGCGAAGC CACTACACCA GTCACTGTGC AGCACATGCG TCCACCCAAA 
CAAAAGAAAC ACAAGACGTC ACCTGTGCCC CCTGCTCCCA TCGTCCTGCA GCTGATGGAG 
ATGGGCTTTC AAAGGAAAAA CATCGAGTTT GCTCTTAAAT CACTGTCTGG AACCACAGGT 
GCTTCTGGAG TGCCAGGTGT CGAGGCATTG GTGGGCTGGT TGTTGGACCA TCCAGATGTT 
CTCATTACAG ATCTGTCGGA TGCAGAGACT GTATCAGATG AATACTCTGA TGAAGAGGTG 
CTGGAAGAGC TTGAGGAGCC TGAGCCTGCC TTTCCTGTGC CTCCTGGGGT TGTGGTGACT 
GAGAGTCAGA CGTTTAAAAA GAGGTCGGAC TTTCAGAGCA ATGACGATTA TGCTGTTTAC 
GTGAGAGAGA ACATTCAGGT GGGCATGATG GTACGGTGTT GCAGGACATA TGAGGAAGTG 
TATGAAGGAG ATGTGGGGAA AGTGATCAAG CTTGACAGAG ACGGCCTGCA TGACCTGAAT 
GTTCAGTGTG ACTGGCAGCA GAAAGGAGGA ACTTACTGGG TCCGTTACAT CCACACTGAG 
CTGCTGGGGT TTCCCCCTCA GAGTTCTCCA TCTCATATAA AGATTGGTGA TAAAGTGCGA 
GTGAAGCCCT CGGTCACAAC ACCAAAATAC AAGTGGGGGT CAGTCACTCA TAGGAGTGTG 
GGTGTGGTCA AAGCCTTCAG TGCCAATGGG AAGGATGTCA TTGTGGACTT TCCTCAGCAG 
TCGCACTGGA CTGGACTGCT GTCAGAGATG GAGCTTGTGC CCAGCGTACA TCCTGGTGTC 
AGGTGTGACG GATGTCAGAT GTTCCCTATA AATGGCCCAA GGTTTAAGTG TCGGAGCTGT 
GATGACTTTG ACTTTTGTGA GAGCTGCTTC AAGACCCGTA AACACAATCC TAGGCATTCA 
TTTGGGAGGA TAAACGAACC AGGTCAGTCA CCCACCTTTA GTGGGCGCTC AGGAAAGCAG 
CTGAAAAGAC ATCACAGCAG TCAGCGTGGC ATGCTGATCG ACGAGTGGTC AAGAGTGGTG 
AAGAACCTCA GCGTATCGTC TTCTGTTAAC CAGGCGTCTC GACTCATCGA CTGTGGAGAG 
CAATGCTGGC AGTCCTCAGG CTCTCAGGGC AAGCACTGGA TCAGATTGGA GCTTTTCCCA 
GACATGTTAG TCCACAAACT GAAAATGATT GTAGATCCAG CTGACAGCAG CTACATGCCT 
TCATTGGTGG TGGTATCAGG TGGAACCTCA CTCAACAATT TGATCGAGTT GAAGACCATC 
AACATCAATC CCACTGACAC TGTCGTCTCT CTACTCAATG ACTGCACAGA GTATCACAGG 
TACATTGAGG TTGCTATAAA GCAGTGTCGA AGTTCAGGTA TTGACTGTAA GATTCATGGC 
TTGAGTGCTG TGGGACGTGT CCGTGCCGAG GATGAAGATC TTGCAACTGT ACCTTTCCTG 
GCATCTGACA ACGAGGAAGA GGAAGATGAG AAGACCGCGA CTGGAAGTAT GTCTCGAAAG 
AGATTGTCAG GCCTCGAGTC TGCAGCCACT ATCAGAACCA AAGTCTTTGT CTGGGGTCTT 
AATGATAAGG ACCAACTTGG AGGACTTAAG GGGTCCAAGA TCAAAGTCCC TTCATTCTCA 
GAGACTCTGT CTGCTCTGAA TGTAGTGCAG GTGGCTGGTG GCTCTAAAAG TCTTTTTGCT 
GTGACTGCTG AAGGTAAAGT ATATGCCTGT GGAGAGGCCA CCAATGGAAG ACTTGGTTTA 
GGCCTCTCCA GTGGAACCAT CCCAATTCCC AGACAGATAT CAGCTCTCAG CAACTATGTG 
GTCAAGAAGG TTGCTGTGCA TTCAGGTGGA CGGCATGCCA TGGCACTTAC CGTGGATGGG 
AAAGTATTCT CTTGGGGTGA AGGAGACGAT GGCAAGCTTG GACACTTCAG CCGAATGAAC 
TGTGATAAGC CAAGGCTGAT CGAGGCATTG AAAACCAAAC GCATTCGAGA TATTGCGTGT 
GGCAGCTCTC ACAGTGCCGC CATCACCTCC AGTGGAGAGC TGTACAGCTG GGGTCTGGGG 
GAATATGGCC GTCTGGGCCA TGGGGACAAC ACCACCCAAC TCAGACCCAA ACTGGTCAAG 
GTGCTTTTAG GCCATCGCGT TGTCCAGGTA GCTTGTGGAA GTAGAGATGC CCAAACTCTT 
GCACTGACAG ATGAAGGAAT GGTGTTCTCA TGGGGAGATG GAGACTTTGG GAAACTGGGC 
CGTGGTGGCA GCGAGGGATG TAACATTCCT CAAAACATTG AGCGTCTGAA TGGACAGGGG 
GTCTGTCAAA TTGAATGTGG AGCTCAATTC TCCCTCGCCC TCACCAAATC TGGAGTTGTC 
TGGACCTGGG GTAAAGGGGA TTACTTCAGA CTGGGTCACG GCACAGATGT TCATGTCCGC 
AAACCCCAGA TGGTTGAAGG ACTTAGGGGG AAGAAGATTG TTCATGTGGC CGTTGGTGCT 
CTGCACTGCC TGGCTGTCAC CGAGACTGGA CAGGTGTTCG CGTGGGGGGA TAATGACCAT 
GGGCAGCAGG GGAATGGCAC AACCACAGTC AACAGGAAGC CCACATTGGT GCAGGGTCTA 
GAGGGTCAGA AAATTACTCG TGTGGCCTGC GGTTCTTCTC ATAGTGTTGC CTGGACCACA 
GTAGATGTCA CTACACCCTC CGTCCATGAG CCTGTGCTCT TCCAGACTGC TAGAGACTCT 
CTGGGAGCCT CTTACCTTGG TGTGCCATCA GATAGTGACC CGTCTTTACT GAGCAATAAG 
CTAAATGGCG TAAACAATGT GAAGCCCAAC AGGCCATCTC TGGCAAAGAT CCTTCTCTCT 
CTGGATGGTA ATCTGGCCAA ACAGCAGGCC CTATCCCATG TGCTGTCTTC CCTGCAGATC 
ATGTATGCCA GAGATGCAGT GGTGGGTGCA TTAATGCCAT CCTGTATGAT GCCTCTGGAG 
TGTCCCTCCA GCTCTCCGGT GCCCCCATCA GACTGTTCAT CAGCTTCCAG CCCCTGTGAG 
GCTGAGGGTC CTGCCCTGCC CTCAGAGAGT GAGGAGAGGG TCAGTCCGCA CCCCTGGATG 
GACAGCAAAC GAGGAGAGGT AACCACGTCT GAGGATGCCA CTACCCCATC TTCAGCAGTG 
ACCCCTTCTA CTGCCTCTGC CTCATCTCGA CCCTTCATAC CAGTCACAGA TGATCCTGGT 
GCTGCCAGCA TCATTGCTGA GACCATGACC AAAACCAAAG AGGACTCTGA GAACCAGAGT 
AAAGCCGTGG GTCCAGAGCC ACAGTATCTG GATGAGTTCA CCAGTCTCCT GATCCCTGAT 
GACACGCGTG TGATGGTGGA CCTGTTAAAA CTAGCTGTAT CTCTGCGCTC TGGTGATAAA 
GGAAAGGAAG TGCTGTCTGC TGTTCTGTCT GGCATGGGTA CAGCCTGCCC ACAGGTGGCG 
GATATGCTTC TGGAGCTGTG TGTGACTGAG TTGGAGGACG TGGCGACTGA CTGTCAGAGT 
GGACGGCTTT CCTCTCAACC TGTGGTGGTG GAAAGCAGCC ATCCATATAT TGACGATACA 
TCCACCAGCG GAACTGTCAA AATACCAGGT GCTGAGGGTT TGCGGGTTGA GTTTGATCGC 
CAGTGCTCAA CAGAAAGGAG ACATGACCCT CTTACCATCA TGGATGGAGC CAATAGAATT 
GTGTCTGTTC GATCAGGTCG TGAGTGGTCT GATTGGTCCA GTGAATTGAG AATTCCAGGA 
GACGAGCTCA AATGGAAGTT CACCAGTGAC GGCTCTGTGA ACGGCTGGGG CTGGCGCTTC 
ACAGTCTATC CCATCATGCC TGCGGCTGGT CCTAAAGATC TTCTGTCAGA CCGCTGCATT 
TTGTCTTGCC CTTCAATGGA TCTAGTAACA TGTCTGCTAG ACTTTCGTCT GAACTTTGCC 
TCAAACCGCA GCATTGTGCC CAGATTAGCA GCCTCACTTG CTGCTTGTGC GCAGCTCAGC 
GCCTTGGCGG CTGGCCATCG TATGTGGGCT CTGCAGAGGT TACGCAAGCT CTTAACTACT 
GAATACGGCC AATCCATTAA CATCAACCGC CTCTTAGGAG ACACCGAAGG AGAGGCTCGC 
TCTATGAGTT TTACAGGAAG TGCATTAGCT GCTCTGGTGA AAGGACTTCC TGAGGCATTA 
CAGAGACAGT ATGAGTACGA GGAGCCCATA GTTAGGGGTG GCAAACAGTT ACTCCACAGC 
CCGTTCTTCA AGGTGTTGGT GGCGCTGGCG TGTGATCTGG AGTTGGACAC TCTTCCATGT 
TGTGCTGAGA CACACAAGTG GGCGTGGTTT CGACGCTATT GCACCGCCTC CAGAGTGTCA 
GTGGCTCTGG ACAAGAGGAG CCCTTTGCCC CGCCCTTTTC TGGATGAGGT AGTGGCAAAG 
AAAATCCGTG AGCTGATGGC AGATCATGAG AACATGAACA CGCTTCATGA GAGCCATGAC 
TTGTTTAAGA GGGAGCAGGA TGAGCAGTTG GTTCAGTGGA TGAACAGACG ACCAGATGAT 
TGGACACTCT CTGCGGGTGG CAGTGGTACA ATATACGGCT GGGGGCACAA TCACCGGGGT 
CAGTTAGGTG GCATTGAAGG AGCCAAAGTA AAGGTTCCCA CACCAACAGA AGCTCTAGCC 
ACACTGCGGC CAGTTCAGCT TATTGGAGGA GAGCAAACAC TGTTTGCCGT CACTGCTGAT 
GGGAAGCTGT ATGCCACAGG ATATGGTGCC GGTGGTCGAC TGGGAATAGG AGGGACAGAG 
TCCGTCTCCA CTCCGACCCT GCTGGAGTCC ATCCAGCATG TTTTCATCAG AAAGGTGGCT 
GTGAACTCTG GAGGGAAACA CTGTTTGGCT CTTTCCTCTG AAGGAGAGCT GTACTCCTGG 
GGGGAGGCCG AAGATGGAAA ACTGGGCCAT GGAAACAGAA GCCCATGTGA TCGTCCACGC 
GTGATTGAGT CTCTCAGAGG GGTGGAAGTG GTGGACATTG CTGCCGGTGG GGCTCACAGT 
GCCTGCATTA CTGCCAGTGG AGAGCTCTAC ACATGGGGCA AAGGTCGCTA TGGGCGCCTG 
GGTCATGGAG ACAGCGAAGA CCAACTTAAA CCAAAACTGG TGGAGGCTCT TCAGGGTCAC 
CGGGTGATAG ATGTGGCATG TGGCAGTGGA GATGCACAGA CCCTCTGTCT GACTGATGAT 
GACATGGTAT GGTCGTGGGG CGATGGAGAC TATGGCAAAT TGGGCCGAGG AGGTAGTGAT 
GGATGTAAAG TGCCGATGAA GATCGATTCA TTAACCGGAT TGGGTGTTGT GAAAGTGGAA 
TGTGGCTCTC AGTTTTCTGT GGCCCTTACC AAGTCTGGAG CAGTTTACAC ATGGGGGAAG 
GGTGACTATC ACAGACTGGG ACACGGCTCA GATGATCATG TTCGCAGACC CCGGCAGGTC 
CAGGGTTTAC AAGGCAAAAA GGTCATCGCA ATAGCAACTG GTTCCCTTCA CTGCGTTTGC 
TGCACGGAGG ACGGGGAAGT GTACACATGG GGTGACAATG ATGAGGGTCA GCTTGGGGAC 
GGTACGACCA ATGCTATTCA GAGACCCAGG CTGGTGGCAG CGCTGCAGGG CAAGAAGATT 
AATCGTGTGG CCTGTGGTTC AGCACATACA CTTGCCTGGT CCACTAGTAA ACCAACCAAT 
GCTGGAAAAC TCCCTGCTCA GGTTCCAATG GAGTACAACC ACCTGCAAGA AATCGCCATC 
ATCGGCCTGA GGAATCGGTT ACTGCTACTG CACCACATCT CTGAGCTCTT CTGCCCCTGC 
ATCCCAATGT TTGACTTAGA AGGACGCCTG GGTGAAACTG GACATGGTGT GTCTGTTGGC 
TTTGACACGC TCCGTGGAAT CCTCATATCA CAGGGCAAGG AGGCAGCATT CCGCAAAGTG 
GTGCAGGCTA CCATGGTCAG AGACAGGCAG CATGGGCCAG TGGTTGAGCT GAACAGGATA 
CAGGTCAAGC GTTCACGTAG TAAAGGAGGT CTGGCAGGGC CGGATGGCAC CAAGTCAGTA 
TTTGGGCAGA TGTGTGCTAA GATGAGTTCT TTCAGTCCAG ACAGTCTGCT GCTGCCACAC 
CGCGTCTGGA AGGTCAAGTT TGTGGGTGAG TCGGTGGATG ACTGTGGTGG CGGATACAGT 
GAATCTATCG CAGAAATGTG CGAGGAGCTT CAGAATGGCC TCACTCCTCT GCTCATCGTC 
ACTCCTAATG GCAGAGACGA GTCAGGAGCG AACAGAGATT GCTTTCTGCT CAACCCAGCT 
GCCAAATCCC CTCTGCACAT CAGCATGTTC CGCTTCCTAG GGGTGCTCCT GGGCATAGCG 
ATCCGAACAG GAAGTCCTCT TAGTCTGTAT CTGGCCGAAC CTGTGTGGAA ACAGCTGGCA 
GGCATGAACC TGACCATTGC TGATCTCAGT GAGGTGGACA AGGACTTCAT CCCCGGTCTG 
ATGTATATTA GAGATAACGA GGCGTCTGCG GAGGAGTTTG AGGCGATGCC TCTTCCCTTC 
ACTGTGCCCA GTGCCTGCGG ACAAGAAGTC CAGCTCAGCT CCAAACATTC CCACATCAGC 
CTTGAAAACC GCAGTGAATA TGTGCGGCTA GCAATAAATT ACAGGCTGCA TGAGTTTGAT 
GAGCAGGTGT CTGCTGTAAG AGAAGGTATG GCGCGTGTGG TTCCGGTACC GCTCCTCTCT 
CTATTTACCG GCTATGAGCT GGAGACCATG GTGTGCGGAA GTCCGGACAT CCCTCTGCAT 
CTGCTCAAAT CAGTGGCCAC ATATAAAGGA GTAGAGCCCA CTGCACCGCT CATCCAGTGG 
TTCTGGGAGG TGATGGAGTC TTTCTCTAAC ACAGAACGCT CGCTCTTCCT CAGGTTCGTC 
TGGGGCCGCA CGCGTCTTCC CAGGACCATC GCAGACTTCC GTGGACGAGA CTTTGTAGTG 
CAGGTGCTGG ATAAATATAA CCCTCCCGAC CACTTCCTCC CCGAGTCCTA CACCTGCTTC 
TTCCTGCTCA AGCTGCCTCG CTACTCCTGC AAGCTAGTCT TGGAGGAGAA ACTCAAATAC 
GCCATTCACT TCTGCAAGTC CATCGACACT GATGACTACG CACGGATCGC TCTGAGCGGA 
GAACCGGCCG CAGACGACAG CAGTGATGAC TCGGACAATG AAGACGCAGA CTCATTCGCT 
TCTGACTCCA CACAGGATTA CCTGACAGGC CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC2 ( XM_021477196.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021332871.1 Danio rerio HECT and RLD domain containing E3 ubiquitin protein ligase 2 (herc2), transcript variant X2, mRNA.

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CloneID	ODa143099
Clone ID Related Accession (Same CDS sequence)	XM_021477196.1
Accession Version	XM_021477196.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 14493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase HERC2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTCTC CATCATTTTC CCTGAATGCC CAGCTGCGCT TCCATGATAA ATGGCTGAAG 
ATCGACTTGC AGCGCGCCTT CTCTCATGAA GGACTGAGCG AGATGTGGAA CGAGATGGTG 
AAGGATGAAG AGATCGTCTT TGACAGAGAA GAGTCTGCCC ATCCAGAGGA CTGTGCTGAT 
TGCACCCAAA AGAAAGATGA CACCAATGAT AAAGAAAAGA AGGAAGAGGA GGAGGCAGCA 
GCAACGGCGG CTTCATCTTT CCATCACTCC ATCATTGAAA TGTGGGACTG GGGGAGGCAG 
CCAGAAACCA GCGAGCTGCG GGACTGTCTG ATGGCTTTGG TAGACGACCA ACAGAAGCTG 
TCTTCTCAGA CGGCTAAGAG CACACTCTCA GCCCTGCGTC TAAGCCAGAG ACTGGTGATC 
CTGGAGCGCT ACCTCATCTC GCTCACTCAC AGCATGATGG AGGAGAAGTA CAAAGTACGG 
TGGAAGAGCC CTCCCAGTCC TCCACTCTCT GCTGTCGACA ACAAAAGCGC TCGGCCAGTT 
AGTAAAGGGG TGGAAGGTTT GGCCAGGGTG GGCTCAAGGG CAGCTCTTTC CTTTGCCTTT 
GCATTTCTCC GCCGAGCCTG GAGATCAGGA GATGATGCAG ACCTGTGCAG TGAGTTACTG 
CAGGAGTCTC TGGATGCTCT GCGGGCTCTT CCAGAAGCTA CTCTGTTTGA TGAGGGCACT 
GTATCTCCTG TTTGGCTGGA GGTGGTTGAG AGGGCTACTA AATTTCTAAG TGACATCCAT 
GGAAGTGCCA GTGGAAAAGC ACCCAGTAAC ATTCCCTTGC AGGATCAGCA CTTGGCCCTT 
GCCATCCTGC TGGAGCTCGC AGTGCAACGA GGAACTCTCA GTCAGCTGCT GTCTGCAGTT 
CTGCTGCTTC TGAGGCTCTG GGACAGTGGA ACTAGAGAGA TGGATAATGA GCGCTCCACC 
CAAGGCACCA GCGCCCCGTT ACTGCCCCTC CTGCAGCGCT TCCAGAACAT CCACAGCAGT 
AAAGAGGAGC CTGTTCCTGA AGAGGAGATT GAGATCCTTT CGGCCCCTCT GAGCCCGAAT 
GAGAGTTTTC TGCGCTACCT TACACTGCCT CAAGATAATG ACCTGGCCAT TGATTTGCGG 
CAGACAGCTG TAGTCATCAT GGCACATTTG GACCGCCTGG CAGCCCCATG CAGCCCCCCG 
CAGTGCAGCT CACCCACTTC TAACAAGGGG ACACTGCAGG AGGTGATTGG TTGGGGTCTG 
CTTGGATGGA AGCCTTATGC TAATGTGAAT GGACCAATTC AGTGCAAGGC ACTATCAAAT 
CTGGGTGTCA CGCAGATTGT CTGCTCAGAG AAGGGTTTCC TCATCCTGAC CATCACTGGT 
GCTGTCTACA CACAAAACTA CAAAAGTACT ACACTAGCAC CTATGCTAGT CCATGCACTG 
ACGTCCAGGA AAATAATGAA GCTTGCAGCT CATCCTGATG GCCAGCATTA TTTGGCTCTT 
TCAGTCAATG GAGAGGTTTT CTCCTGGGGT TGTGGAGATG GAGGCAGGCT AGGACATGGA 
GACACTACGT ATTTAGAGGA GCCCACTATG ATTTCAGTAT TCTCAGGGAG GCCGGTAGGG 
AAGCAGGTTG TACATATTGC CTGTGGCAGC ACATACAGCG CAGCCATCAC AGCAGATGGA 
GAGCTTCACA CATGGGGCCG AGGAAACTAC GGCAGACTGG GCCATGGCTC CAGTGAGGAC 
CAGACCACTC CCATGCTAGT AACTGCACTG AAGGGGCTGA AGGTGATCGA TGTGGCCTGT 
GGAAGCGGAG ATGCTCAGAC GCTTGCTGTG ACTGAGAATG GTCAGGTATG GTCATGGGGT 
GATGGAGATT ATGGGAAACT GGGCCGAGGG GGCAGTGATG GATGCAAAAC ACCAAAACTA 
GTGGAGAAGC TCCAGGATCT GGATATTGTG AAGGTGTGCT GTGGGAGCCA GTTCTCCGTA 
GCGCTCACTA AAGATGGACA AGTTTACACC TGGGGCAAAG GAGACAATCA ACGACTTGGA 
CATGGCACTG ATGAACATGT CCGCTATCCA AAACTACTTG ACAGTCTGCA AGGCAAGAGG 
GTGGTGGACA TAGCGGTTGG CTCCACACAC TGCCTGGCCC TTACAGAAGA AGGAGAGGTG 
CACAGCTGGG GCAGTAATGA CAAACTACAG CACTTTGACA CACTTTTCTC CAATAAGAAG 
CAGCCCAAAG CCTTGCCAGG CCTCAACTCC AAACACATAG TAGGCATCTC CTGCGGTCCT 
GGCCAGAGTT TTTCATGGTC ATCCTGCTCT GAGTGGTCTG TGGGTCTCCG GGTACCCTTC 
GTGGTAGACG TGTGCACTAT GACCTTTGAC CAGCTGGACC TGCTTCTGCG CCAGGTTAGT 
GAGGGCATGG ACGGCAGCTC TGATTGGCCA CCACCACAGG AGAAAGAATG CATGGTCGTG 
GCAGCTCTCA ACCTGCTCAG ACTACAGCTT CATGCAGCCA TCAGTAATCA GGTGGATCCC 
GAGGAGCTGG GTCTGGGTCT GGGCAGTGTG CTGCTTAATA ACCTCAAGCA GACGGTGGTG 
ACTCTGGCCA GCAACGGCGG GGTGCTAAAC ACTGTGCAGG CTGCCGCCCA GACCGTGCTC 
CAGAGTGGCT GGTCAGTGCT ACTCCCCACT GCAGAGGAAC GAGCAAGAGC CCTGTCCTCA 
CTGCTGCCCA ATGCAGCATC TGCTAATGAG ATGAGTGTAA GTCCTGGCCG TCGCTTCATG 
ATCGACCTCT TGGTTAGTAG TCTGATGGCA GATGGAGGGT TGGAGTCTGC TTTGAATGCT 
GCCATCACGG CCGAGATCCA GGATATTGAG GCAAAGAAAG AGGCTCAGAA AGAGAAGGAA 
ATCGATGAGC AGGAAGCCAG CGCCTCTTCT TTGCATCGCT GTCGCACCAC ACTGGACAAA 
GACCTGATCA ACACCGGCAT CTATGAGTCT GCCAACAAAC AGAGCCTCCC ATTGGTCCAG 
CTTGTCCAAC AGCTGCTCAG GAACATTGCT TCACAGACTA TAGCCCGTCT TAAGGATGTG 
GCCCGACGCA TCTCAAATTG TCAAGACACC GAGCAGGTCA GCAAGGAGCG CTCAGCCTCT 
CTGGACCTGC TGCTGCGCTT TCAGAGGCTT TTGGTCTGCA AATTGTATGT CGGAGTCAAT 
GGCACTGTGG GTGTGGGAGG ATACACTTTG AGTCACTCAG AAATTCTGGG AGTTGGCTCT 
CTCCTGAAGA AGTATATGGC TTTGCTTTGC ACACACATCG GTGATATCCT TCCTGTGGCC 
ACCAGCATTG CCCTCAACAG CCATCGTCAC TTTGCAGAGG TGGCTCGTGT CCTTGAGGGA 
GATCTTACAG GTGTTCTGCT ACCAGAGCTC ATTGTGTCCA TTGTGCTGCT GCTGACCCAT 
GATGCTGGAC TAATGCAAGA GACTGGATCC ATTCCTCTGC TCGCTGCTCT TCTGGAGCAC 
TTGGACCGTT TTAACTATCT TGCACCTGGT ACAGAAAGAG ATGACAATGA GGATCTTGCC 
TGGCCTGGAC TCATGGGTTT CTTTTTCAAT GGACCGAGCT CAAAGAACAA CGAGGAGGTG 
TCACTTATCC GCAAAGCAGA CCTGGAGAAT CACAACAAAG ATGGCGGCTT TTGGACTGTA 
ATTGAAGGGA AGGTCTATGA CATAAAGGAC TTTCAAGCTC AGACTCTAAC TGGCAACAGC 
ATCCTTGCCC AGTTTGCAGG AGAAGACCCT GTGGTGGCAT TAGAGGCTGC CCTCCAATTT 
GAAGACACCA GAGAGTCCAT GCAAGCTTTC TGTGTGGGAC AGTATATGGA GCCCAACCAA 
GAAATGGTGA CAACGCCTGA CCTGAGCAGC CTGTCCTCTC CTCTTATAGA TGCTGAGAGG 
AACCTTGGTC TGCTTCTGGG GCTACATGCC TCATATCTGG CTATGAGCAC CCCTCTCTCC 
CGCATAGAGT TAGAATGTGC CAAATGGCTC CAGTCATCTA TTTTCTTCGG TGGACTTCAG 
ACCAGTCAGA TCCACTATAA TTATAACGAG GAGAAGGATG AGGACCACTG CAGCTCTCCA 
GGGACCACCA CACCAGACAA AGCCAAACTG TACTGCCGCA GAATCACCCT GAGTGATCAC 
GCCCAGCCCT TCCTGCAGGC CATCGCTGAC AACACCACCC AGGATCACAC TGTGAAGGAC 
TTTTTATGCC AAATCGAGCG GTGCTGTAAA CAGTACCATC TCATCACTCC CATCACTTTT 
CCACCTGAAC ATCCTGTGGA GGAAGTGGGT CGCCTGCTGC TCTGCTGCCT TCTGAAGCAT 
CAGGATCTTG GTCATGTCGC CCTCTCCCTA GTTGATCAAT GTGCCTTGGG TATGGACCAG 
GGGAAACAGA GGTCCCTCCC GAAATCTGTG ATTGATGTGT GTCGAATGGT GTATCAGGCC 
AAGTGTTCGT TGATAAAAAC ACACCAGGAA CAGGGTCGTT CTTATAAAGA GGTGTGCGCT 
CCTGTTATTG AGAGGTTGCG TTTCCTGTTT AATGAGCTAC GACCTGCCGT CAGTAATGAC 
TTGTCCATTG TGTCCAAACT CAAACTGCTG AGCTCTCAGC CTCGCTGGAA GAGGATCACA 
CAGAAACTGA TCAGAGACCG CCGCAAAAAG AGAGTGCCTA AAAAGTCGGA GTCAGCTGAT 
CTGGAGGAGT GCAAGATGGA AGGGACCAAT GAGGAACCAC CTGCTCCTCT AAGCCCCGCT 
CCTGTTGACA AGAAGCAAAC AACTGTCAAA TCTGCAAAGC AGGATAAGTG GCAGCCTCTG 
CTAAACACCA TCGGCAGTGT TCAGAAGTAC CGCTGGTTAA GGCACAGCGT TCAGGGCTCC 
TTCTCTCAGT CCAGCCTCAT GAGCACCATC GTTGAGTTTT CTTTGAAAGA GGAGCCGCTG 
GATGTGGAGA AGATCAGAAA GTGCCTCCTT AAACAGCTGG AGAGAGCTGA GGTCCGCCTG 
GAGGGTATTG ACACTATGTT GAAGCTGGCA TCTAAAAACT TCCTCCTGCC CTCTGTTCAG 
TATGCCATGC TTTGTGGCTG GCAGAGAGTC ATACCTGAAG GGACCAATAT TGGAGAGCCA 
TTGGCTGACT GTCTAAAGGA TGTAGATCTC ATTCCTCCAT TCAACCGCAT GCTACTAGAG 
GTGACGTTTG GGAAGCTCTA TGCCTGGTCC ATACAGAACG TCCGCAACAT CTTGTTGGAG 
GCTAGTGTCA AGTTCAAGGA ACTGGGTGTG CAACCAGTGC CTCTCCAGAC AATCACTAAT 
GAGAACCCAG CAGGCCCCAG TCTGGGTACC ATCCCTCAGG CACGCTTCCT GCTGGCTATG 
CTCCATATGC TCACTCTCAA ACACGGAGCC AACAGCCTCA GCCTCCTGCT GAACTCTGGC 
ATCCTCGCTC TCACCCAGAG TGTCCTCCGA CTCATTGGTC CTAGCTGTGA TAGCAGTGAG 
GAGGAGCTGA CCTCTGTAGC TCATGGAGGC TCTGCCACAG TTCTGGAAGA GTCCAGAAAG 
GAGACTGCCC CGACCCCTCT GCCCGCTTCC GGCCCTGAGC TGGCAGCTAT GATGAAGATC 
AGCACAAGGG TGATGAGAGG TCCTGACTGG AAGTGGGGGG ACCAGGATGG CCCATCACCA 
GGCCTGGGTC GTGTGATTGG GGAGTTGGGA GAAGATGGTT GGATAAGAGT CCAATGGGAC 
ACCGGCAGCA CCAACTCCTA CAGGATGGGA AAAGAGGGCA AGTATGACCT TAAACTGGCT 
GAGCCGCCAC CTGCCACCCA ACCAGCCACT GAAGACTCTG ACACGGAGGA TGACACAGAA 
GGGGAGGTGA TGGAAAAGTG TGCCCACCCC ACGGCAATCA TGCTGACTAG TACAGTAAAT 
CTGTTGAAGA CACTGTCACT GTCTTCTGGG ATTCATGCAG AGGTGATGCA GGTCGATGCG 
GTCAGTACTC TGTGCGGGCT ACTCAGGATA CTGGTGGAGA GTGGAGCCAA CGACAAGACT 
GGTTGCTCCT CTCACAAGCT GGTGTCCCGA GAGCAGCACA GGAGCTGGTG CACGCTGGGA 
TTTATTCGCA GTATAGCTCT TATGCCTCAG ATGTGCAGTA CCCTGAGCTC CTCTGCCTGG 
ATCAATCTGC TTATACGCAT CGTGGAGGGT CATCAGTCCT TCACTGCAAT CACACTACAG 
AGACAGATTC TTGCCCTGCG GCTTCTACAG GCAGTATTGC CCTCCTGGGA TAAGACTGAA 
CGCTCTCAGG ATATGAAGTT CTTGGTGGAG AAACTGTTTG GGTTCCTCGG CAGCTTGCTC 
AGCACTTGTT CTTCAGATCT GCCTCTTCTT AGGGAAGGTT CAATGCGTAG GAGGAAGTCC 
CGCCCCCAGG CCTCACTGAC AGCCACTCAT AGCAGTACTT TGGCAGAGGA AATAGTGACA 
GTATTGCGCA CGCTTCACTC CCTCAGCCAA TGGAATGCCC TGATTAATGA ATACATTAAC 
TCTCAGCTCA GTGGAATCGG GGATGTCATG GCAGGCCGAC ACACTGAAGT GGCTTTGCTT 
GAAGACTATC TCCCCAATGC GGAAGCAGCA GCGGTGGGCA GTCTGATGGC TGTTTTGGCA 
GTCATCGGGG GGATTGATGG ACGTCTGCGT CTGGGTGGAC GTGTGGTCCA TGAGGAGTAT 
GGAGAAGGCA CAGTGACTCG CATCACACCT AAAGGACGAA TCACTGTTCA GTTTCATGAG 
ATGAGGAACT GCAAAGTGTG CCTGCTCAGT CAGCTCAAAC CGCTTCACAT GATTCCATTC 
AGCGTTCAGA ATCTGCCCTT CGCTGAGCCC ATGCTAATGG TGTGGGCCCA GCTAGTCAAC 
TTGGCTGGGA GCAAGCTGGA GAAACAACGC ATGAAGAAAT CTCTTAGTAG AGGACTTACA 
GCGGATCAGG TAGATGTACA TTTGTTGCGC TGTCAGCAGC TCAGGCTGTA CATCTTGAAG 
GCTGCACGAG CTCTACTCTC CCATCAGGAC AAACTCAGAC AGATCCTTTC TCAGGCGGCC 
GTGGTTGATG TCAGCCCCAA TCCTACAGAG GATCAGTGCG CGTCGTCTCC TGATGTAGGA 
GATTTGTCTC CTGAGGGTCC TCAGCCTCCT CTCATCCTCC TTCAGCAGTT GCTGTCAGCA 
TCCACTCAAC CTTCACCCAT CAAAGCCATA TTCCACCGCC AGGAGTTAGA GGCAGCGGCT 
CTGGCTGTGT GTCAGTACCT GGCGGTTGAA TCCACACACC CGTCATCGCC TCTGTTTGAG 
GACAGCAGCT CTAGCGAAGC CACTACACCA GTCACTGTGC AGCACATGCG TCCACCCAAA 
CAAAAGAAAC ACAAGACGTC ACCTGTGCCC CCTGCTCCCA TCGTCCTGCA GCTGATGGAG 
ATGGGCTTTC AAAGGAAAAA CATCGAGTTT GCTCTTAAAT CACTGTCTGG AACCACAGGT 
GCTTCTGGAG TGCCAGGTGT CGAGGCATTG GTGGGCTGGT TGTTGGACCA TCCAGATGTT 
CTCATTACAG ATCTGTCGGA TGCAGAGACT GTATCAGATG AATACTCTGA TGAAGAGGTG 
CTGGAAGAGC TTGAGGAGCC TGAGCCTGCC TTTCCTGTGC CTCCTGGGGT TGTGGTGACT 
GAGAGTCAGA CGTTTAAAAA GAGGTCGGAC TTTCAGAGCA ATGACGATTA TGCTGTTTAC 
GTGAGAGAGA ACATTCAGGT GGGCATGATG GTACGGTGTT GCAGGACATA TGAGGAAGTG 
TATGAAGGAG ATGTGGGGAA AGTGATCAAG CTTGACAGAG ACGGCCTGCA TGACCTGAAT 
GTTCAGTGTG ACTGGCAGCA GAAAGGAGGA ACTTACTGGG TCCGTTACAT CCACACTGAG 
CTGCTGGGGT TTCCCCCTCA GAGTTCTCCA TCTCATATAA AGATTGGTGA TAAAGTGCGA 
GTGAAGCCCT CGGTCACAAC ACCAAAATAC AAGTGGGGGT CAGTCACTCA TAGGAGTGTG 
GGTGTGGTCA AAGCCTTCAG TGCCAATGGG AAGGATGTCA TTGTGGACTT TCCTCAGCAG 
TCGCACTGGA CTGGACTGCT GTCAGAGATG GAGCTTGTGC CCAGCGTACA TCCTGGTGTC 
AGGTGTGACG GATGTCAGAT GTTCCCTATA AATGGCCCAA GGTTTAAGTG TCGGAGCTGT 
GATGACTTTG ACTTTTGTGA GAGCTGCTTC AAGACCCGTA AACACAATCC TAGGCATTCA 
TTTGGGAGGA TAAACGAACC AGGTCAGTCA CCCACCTTTA GTGGGCGCTC AGGAAAGCAG 
CTGAAAAGAC ATCACAGCAG TCAGCGTGGC ATGCTGATCG ACGAGTGGTC AAGAGTGGTG 
AAGAACCTCA GCGTATCGTC TTCTGTTAAC CAGGCGTCTC GACTCATCGA CTGTGGAGAG 
CAATGCTGGC AGTCCTCAGG CTCTCAGGGC AAGCACTGGA TCAGATTGGA GCTTTTCCCA 
GACATGTTAG TCCACAAACT GAAAATGATT GTAGATCCAG CTGACAGCAG CTACATGCCT 
TCATTGGTGG TGGTATCAGG TGGAACCTCA CTCAACAATT TGATCGAGTT GAAGACCATC 
AACATCAATC CCACTGACAC TGTCGTCTCT CTACTCAATG ACTGCACAGA GTATCACAGG 
TACATTGAGG TTGCTATAAA GCAGTGTCGA AGTTCAGGTA TTGACTGTAA GATTCATGGC 
TTGAGTGCTG TGGGACGTGT CCGTGCCGAG GATGAAGATC TTGCAACTGT ACCTTTCCTG 
GCATCTGACA ACGAGGAAGA GGAAGATGAG AAGACCGCGA CTGGAAGTAT GTCTCGAAAG 
AGATTGTCAG GCCTCGAGTC TGCAGCCACT ATCAGAACCA AAGTCTTTGT CTGGGGTCTT 
AATGATAAGG ACCAACTTGG AGGACTTAAG GGGTCCAAGA TCAAAGTCCC TTCATTCTCA 
GAGACTCTGT CTGCTCTGAA TGTAGTGCAG GTGGCTGGTG GCTCTAAAAG TCTTTTTGCT 
GTGACTGCTG AAGGTAAAGT ATATGCCTGT GGAGAGGCCA CCAATGGAAG ACTTGGTTTA 
GGCCTCTCCA GTGGAACCAT CCCAATTCCC AGACAGATAT CAGCTCTCAG CAACTATGTG 
GTCAAGAAGG TTGCTGTGCA TTCAGGTGGA CGGCATGCCA TGGCACTTAC CGTGGATGGG 
AAAGTATTCT CTTGGGGTGA AGGAGACGAT GGCAAGCTTG GACACTTCAG CCGAATGAAC 
TGTGATAAGC CAAGGCTGAT CGAGGCATTG AAAACCAAAC GCATTCGAGA TATTGCGTGT 
GGCAGCTCTC ACAGTGCCGC CATCACCTCC AGTGGAGAGC TGTACAGCTG GGGTCTGGGG 
GAATATGGCC GTCTGGGCCA TGGGGACAAC ACCACCCAAC TCAGACCCAA ACTGGTCAAG 
GTGCTTTTAG GCCATCGCGT TGTCCAGGTA GCTTGTGGAA GTAGAGATGC CCAAACTCTT 
GCACTGACAG ATGAAGGAAT GGTGTTCTCA TGGGGAGATG GAGACTTTGG GAAACTGGGC 
CGTGGTGGCA GCGAGGGATG TAACATTCCT CAAAACATTG AGCGTCTGAA TGGACAGGGG 
GTCTGTCAAA TTGAATGTGG AGCTCAATTC TCCCTCGCCC TCACCAAATC TGGAGTTGTC 
TGGACCTGGG GTAAAGGGGA TTACTTCAGA CTGGGTCACG GCACAGATGT TCATGTCCGC 
AAACCCCAGA TGGTTGAAGG ACTTAGGGGG AAGAAGATTG TTCATGTGGC CGTTGGTGCT 
CTGCACTGCC TGGCTGTCAC CGAGACTGGA CAGGTGTTCG CGTGGGGGGA TAATGACCAT 
GGGCAGCAGG GGAATGGCAC AACCACAGTC AACAGGAAGC CCACATTGGT GCAGGGTCTA 
GAGGGTCAGA AAATTACTCG TGTGGCCTGC GGTTCTTCTC ATAGTGTTGC CTGGACCACA 
GTAGATGTCA CTACACCCTC CGTCCATGAG CCTGTGCTCT TCCAGACTGC TAGAGACTCT 
CTGGGAGCCT CTTACCTTGG TGTGCCATCA GATAGTGACC CGTCTTTACT GAGCAATAAG 
CTAAATGGCG TAAACAATGT GAAGCCCAAC AGGCCATCTC TGGCAAAGAT CCTTCTCTCT 
CTGGATGGTA ATCTGGCCAA ACAGCAGGCC CTATCCCATG TGCTGTCTTC CCTGCAGATC 
ATGTATGCCA GAGATGCAGT GGTGGGTGCA TTAATGCCAT CCTGTATGAT GCCTCTGGAG 
TGTCCCTCCA GCTCTCCGGT GCCCCCATCA GACTGTTCAT CAGCTTCCAG CCCCTGTGAG 
GCTGAGGGTC CTGCCCTGCC CTCAGAGAGT GAGGAGAGGG TCAGTCCGCA CCCCTGGATG 
GACAGCAAAC GAGGAGAGGT AACCACGTCT GAGGATGCCA CTACCCCATC TTCAGCAGTG 
ACCCCTTCTA CTGCCTCTGC CTCATCTCGA CCCTTCATAC CAGTCACAGA TGATCCTGGT 
GCTGCCAGCA TCATTGCTGA GACCATGACC AAAACCAAAG AGGACTCTGA GAACCAGAGT 
AAAGCCGTGG GTCCAGAGCC ACAGTATCTG GATGAGTTCA CCAGTCTCCT GATCCCTGAT 
GACACGCGTG TGATGGTGGA CCTGTTAAAA CTAGCTGTAT CTCTGCGCTC TGGTGATAAA 
GGAAAGGAAG TGCTGTCTGC TGTTCTGTCT GGCATGGGTA CAGCCTGCCC ACAGGTGGCG 
GATATGCTTC TGGAGCTGTG TGTGACTGAG TTGGAGGACG TGGCGACTGA CTGTCAGAGT 
GGACGGCTTT CCTCTCAACC TGTGGTGGTG GAAAGCAGCC ATCCATATAT TGACGATACA 
TCCACCAGCG GAACTGTCAA AATACCAGGT GCTGAGGGTT TGCGGGTTGA GTTTGATCGC 
CAGTGCTCAA CAGAAAGGAG ACATGACCCT CTTACCATCA TGGATGGAGC CAATAGAATT 
GTGTCTGTTC GATCAGGTCG TGAGTGGTCT GATTGGTCCA GTGAATTGAG AATTCCAGGA 
GACGAGCTCA AATGGAAGTT CACCAGTGAC GGCTCTGTGA ACGGCTGGGG CTGGCGCTTC 
ACAGTCTATC CCATCATGCC TGCGGCTGGT CCTAAAGATC TTCTGTCAGA CCGCTGCATT 
TTGTCTTGCC CTTCAATGGA TCTAGTAACA TGTCTGCTAG ACTTTCGTCT GAACTTTGCC 
TCAAACCGCA GCATTGTGCC CAGATTAGCA GCCTCACTTG CTGCTTGTGC GCAGCTCAGC 
GCCTTGGCGG CTGGCCATCG TATGTGGGCT CTGCAGAGGT TACGCAAGCT CTTAACTACT 
GAATACGGCC AATCCATTAA CATCAACCGC CTCTTAGGAG ACACCGAAGG AGAGGCTCGC 
TCTATGAGTT TTACAGGAAG TGCATTAGCT GCTCTGGTGA AAGGACTTCC TGAGGCATTA 
CAGAGACAGT ATGAGTACGA GGAGCCCATA GTTAGGGGTG GCAAACAGTT ACTCCACAGC 
CCGTTCTTCA AGGTGTTGGT GGCGCTGGCG TGTGATCTGG AGTTGGACAC TCTTCCATGT 
TGTGCTGAGA CACACAAGTG GGCGTGGTTT CGACGCTATT GCACCGCCTC CAGAGTGTCA 
GTGGCTCTGG ACAAGAGGAG CCCTTTGCCC CGCCCTTTTC TGGATGAGGT GGCAAAGAAA 
ATCCGTGAGC TGATGGCAGA TCATGAGAAC ATGAACACGC TTCATGAGAG CCATGACTTG 
TTTAAGAGGG AGCAGGATGA GCAGTTGGTT CAGTGGATGA ACAGACGACC AGATGATTGG 
ACACTCTCTG CGGGTGGCAG TGGTACAATA TACGGCTGGG GGCACAATCA CCGGGGTCAG 
TTAGGTGGCA TTGAAGGAGC CAAAGTAAAG GTTCCCACAC CAACAGAAGC TCTAGCCACA 
CTGCGGCCAG TTCAGCTTAT TGGAGGAGAG CAAACACTGT TTGCCGTCAC TGCTGATGGG 
AAGCTGTATG CCACAGGATA TGGTGCCGGT GGTCGACTGG GAATAGGAGG GACAGAGTCC 
GTCTCCACTC CGACCCTGCT GGAGTCCATC CAGCATGTTT TCATCAGAAA GGTGGCTGTG 
AACTCTGGAG GGAAACACTG TTTGGCTCTT TCCTCTGAAG GAGAGCTGTA CTCCTGGGGG 
GAGGCCGAAG ATGGAAAACT GGGCCATGGA AACAGAAGCC CATGTGATCG TCCACGCGTG 
ATTGAGTCTC TCAGAGGGGT GGAAGTGGTG GACATTGCTG CCGGTGGGGC TCACAGTGCC 
TGCATTACTG CCAGTGGAGA GCTCTACACA TGGGGCAAAG GTCGCTATGG GCGCCTGGGT 
CATGGAGACA GCGAAGACCA ACTTAAACCA AAACTGGTGG AGGCTCTTCA GGGTCACCGG 
GTGATAGATG TGGCATGTGG CAGTGGAGAT GCACAGACCC TCTGTCTGAC TGATGATGAC 
ATGGTATGGT CGTGGGGCGA TGGAGACTAT GGCAAATTGG GCCGAGGAGG TAGTGATGGA 
TGTAAAGTGC CGATGAAGAT CGATTCATTA ACCGGATTGG GTGTTGTGAA AGTGGAATGT 
GGCTCTCAGT TTTCTGTGGC CCTTACCAAG TCTGGAGCAG TTTACACATG GGGGAAGGGT 
GACTATCACA GACTGGGACA CGGCTCAGAT GATCATGTTC GCAGACCCCG GCAGGTCCAG 
GGTTTACAAG GCAAAAAGGT CATCGCAATA GCAACTGGTT CCCTTCACTG CGTTTGCTGC 
ACGGAGGACG GGGAAGTGTA CACATGGGGT GACAATGATG AGGGTCAGCT TGGGGACGGT 
ACGACCAATG CTATTCAGAG ACCCAGGCTG GTGGCAGCGC TGCAGGGCAA GAAGATTAAT 
CGTGTGGCCT GTGGTTCAGC ACATACACTT GCCTGGTCCA CTAGTAAACC AACCAATGCT 
GGAAAACTCC CTGCTCAGGT TCCAATGGAG TACAACCACC TGCAAGAAAT CGCCATCATC 
GGCCTGAGGA ATCGGTTACT GCTACTGCAC CACATCTCTG AGCTCTTCTG CCCCTGCATC 
CCAATGTTTG ACTTAGAAGG ACGCCTGGGT GAAACTGGAC ATGGTGTGTC TGTTGGCTTT 
GACACGCTCC GTGGAATCCT CATATCACAG GGCAAGGAGG CAGCATTCCG CAAAGTGGTG 
CAGGCTACCA TGGTCAGAGA CAGGCAGCAT GGGCCAGTGG TTGAGCTGAA CAGGATACAG 
GTCAAGCGTT CACGTAGTAA AGGAGGTCTG GCAGGGCCGG ATGGCACCAA GTCAGTATTT 
GGGCAGATGT GTGCTAAGAT GAGTTCTTTC AGTCCAGACA GTCTGCTGCT GCCACACCGC 
GTCTGGAAGG TCAAGTTTGT GGGTGAGTCG GTGGATGACT GTGGTGGCGG ATACAGTGAA 
TCTATCGCAG AAATGTGCGA GGAGCTTCAG AATGGCCTCA CTCCTCTGCT CATCGTCACT 
CCTAATGGCA GAGACGAGTC AGGAGCGAAC AGAGATTGCT TTCTGCTCAA CCCAGCTGCC 
AAATCCCCTC TGCACATCAG CATGTTCCGC TTCCTAGGGG TGCTCCTGGG CATAGCGATC 
CGAACAGGAA GTCCTCTTAG TCTGTATCTG GCCGAACCTG TGTGGAAACA GCTGGCAGGC 
ATGAACCTGA CCATTGCTGA TCTCAGTGAG GTGGACAAGG ACTTCATCCC CGGTCTGATG 
TATATTAGAG ATAACGAGGC GTCTGCGGAG GAGTTTGAGG CGATGCCTCT TCCCTTCACT 
GTGCCCAGTG CCTGCGGACA AGAAGTCCAG CTCAGCTCCA AACATTCCCA CATCAGCCTT 
GAAAACCGCA GTGAATATGT GCGGCTAGCA ATAAATTACA GGCTGCATGA GTTTGATGAG 
CAGGTGTCTG CTGTAAGAGA AGGTATGGCG CGTGTGGTTC CGGTACCGCT CCTCTCTCTA 
TTTACCGGCT ATGAGCTGGA GACCATGGTG TGCGGAAGTC CGGACATCCC TCTGCATCTG 
CTCAAATCAG TGGCCACATA TAAAGGAGTA GAGCCCACTG CACCGCTCAT CCAGTGGTTC 
TGGGAGGTGA TGGAGTCTTT CTCTAACACA GAACGCTCGC TCTTCCTCAG GTTCGTCTGG 
GGCCGCACGC GTCTTCCCAG GACCATCGCA GACTTCCGTG GACGAGACTT TGTAGTGCAG 
GTGCTGGATA AATATAACCC TCCCGACCAC TTCCTCCCCG AGTCCTACAC CTGCTTCTTC 
CTGCTCAAGC TGCCTCGCTA CTCCTGCAAG CTAGTCTTGG AGGAGAAACT CAAATACGCC 
ATTCACTTCT GCAAGTCCAT CGACACTGAT GACTACGCAC GGATCGCTCT GAGCGGAGAA 
CCGGCCGCAG ACGACAGCAG TGATGACTCG GACAATGAAG ACGCAGACTC ATTCGCTTCT 
GACTCCACAC AGGATTACCT GACAGGCCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332871.1
CDS	149..14641
Translation	MPSPSFSLNAQLRFHDKWLKIDLQRAFSHEGLSEMWNEMVKDEEIVFDREESAHPEDCADCTQKKDDTNDKEKKEEEEAAATAASSFHHSIIEMWDWGRQPETSELRDCLMALVDDQQKLSSQTAKSTLSALRLSQRLVILERYLISLTHSMMEEKYKVRWKSPPSPPLSAVDNKSARPVSKGVEGLARVGSRAALSFAFAFLRRAWRSGDDADLCSELLQESLDALRALPEATLFDEGTVSPVWLEVVERATKFLSDIHGSASGKAPSNIPLQDQHLALAILLELAVQRGTLSQLLSAVLLLLRLWDSGTREMDNERSTQGTSAPLLPLLQRFQNIHSSKEEPVPEEEIEILSAPLSPNESFLRYLTLPQDNDLAIDLRQTAVVIMAHLDRLAAPCSPPQCSSPTSNKGTLQEVIGWGLLGWKPYANVNGPIQCKALSNLGVTQIVCSEKGFLILTITGAVYTQNYKSTTLAPMLVHALTSRKIMKLAAHPDGQHYLALSVNGEVFSWGCGDGGRLGHGDTTYLEEPTMISVFSGRPVGKQVVHIACGSTYSAAITADGELHTWGRGNYGRLGHGSSEDQTTPMLVTALKGLKVIDVACGSGDAQTLAVTENGQVWSWGDGDYGKLGRGGSDGCKTPKLVEKLQDLDIVKVCCGSQFSVALTKDGQVYTWGKGDNQRLGHGTDEHVRYPKLLDSLQGKRVVDIAVGSTHCLALTEEGEVHSWGSNDKLQHFDTLFSNKKQPKALPGLNSKHIVGISCGPGQSFSWSSCSEWSVGLRVPFVVDVCTMTFDQLDLLLRQVSEGMDGSSDWPPPQEKECMVVAALNLLRLQLHAAISNQVDPEELGLGLGSVLLNNLKQTVVTLASNGGVLNTVQAAAQTVLQSGWSVLLPTAEERARALSSLLPNAASANEMSVSPGRRFMIDLLVSSLMADGGLESALNAAITAEIQDIEAKKEAQKEKEIDEQEASASSLHRCRTTLDKDLINTGIYESANKQSLPLVQLVQQLLRNIASQTIARLKDVARRISNCQDTEQVSKERSASLDLLLRFQRLLVCKLYVGVNGTVGVGGYTLSHSEILGVGSLLKKYMALLCTHIGDILPVATSIALNSHRHFAEVARVLEGDLTGVLLPELIVSIVLLLTHDAGLMQETGSIPLLAALLEHLDRFNYLAPGTERDDNEDLAWPGLMGFFFNGPSSKNNEEVSLIRKADLENHNKDGGFWTVIEGKVYDIKDFQAQTLTGNSILAQFAGEDPVVALEAALQFEDTRESMQAFCVGQYMEPNQEMVTTPDLSSLSSPLIDAERNLGLLLGLHASYLAMSTPLSRIELECAKWLQSSIFFGGLQTSQIHYNYNEEKDEDHCSSPGTTTPDKAKLYCRRITLSDHAQPFLQAIADNTTQDHTVKDFLCQIERCCKQYHLITPITFPPEHPVEEVGRLLLCCLLKHQDLGHVALSLVDQCALGMDQGKQRSLPKSVIDVCRMVYQAKCSLIKTHQEQGRSYKEVCAPVIERLRFLFNELRPAVSNDLSIVSKLKLLSSQPRWKRITQKLIRDRRKKRVPKKSESADLEECKMEGTNEEPPAPLSPAPVDKKQTTVKSAKQDKWQPLLNTIGSVQKYRWLRHSVQGSFSQSSLMSTIVEFSLKEEPLDVEKIRKCLLKQLERAEVRLEGIDTMLKLASKNFLLPSVQYAMLCGWQRVIPEGTNIGEPLADCLKDVDLIPPFNRMLLEVTFGKLYAWSIQNVRNILLEASVKFKELGVQPVPLQTITNENPAGPSLGTIPQARFLLAMLHMLTLKHGANSLSLLLNSGILALTQSVLRLIGPSCDSSEEELTSVAHGGSATVLEESRKETAPTPLPASGPELAAMMKISTRVMRGPDWKWGDQDGPSPGLGRVIGELGEDGWIRVQWDTGSTNSYRMGKEGKYDLKLAEPPPATQPATEDSDTEDDTEGEVMEKCAHPTAIMLTSTVNLLKTLSLSSGIHAEVMQVDAVSTLCGLLRILVESGANDKTGCSSHKLVSREQHRSWCTLGFIRSIALMPQMCSTLSSSAWINLLIRIVEGHQSFTAITLQRQILALRLLQAVLPSWDKTERSQDMKFLVEKLFGFLGSLLSTCSSDLPLLREGSMRRRKSRPQASLTATHSSTLAEEIVTVLRTLHSLSQWNALINEYINSQLSGIGDVMAGRHTEVALLEDYLPNAEAAAVGSLMAVLAVIGGIDGRLRLGGRVVHEEYGEGTVTRITPKGRITVQFHEMRNCKVCLLSQLKPLHMIPFSVQNLPFAEPMLMVWAQLVNLAGSKLEKQRMKKSLSRGLTADQVDVHLLRCQQLRLYILKAARALLSHQDKLRQILSQAAVVDVSPNPTEDQCASSPDVGDLSPEGPQPPLILLQQLLSASTQPSPIKAIFHRQELEAAALAVCQYLAVESTHPSSPLFEDSSSSEATTPVTVQHMRPPKQKKHKTSPVPPAPIVLQLMEMGFQRKNIEFALKSLSGTTGASGVPGVEALVGWLLDHPDVLITDLSDAETVSDEYSDEEVLEELEEPEPAFPVPPGVVVTESQTFKKRSDFQSNDDYAVYVRENIQVGMMVRCCRTYEEVYEGDVGKVIKLDRDGLHDLNVQCDWQQKGGTYWVRYIHTELLGFPPQSSPSHIKIGDKVRVKPSVTTPKYKWGSVTHRSVGVVKAFSANGKDVIVDFPQQSHWTGLLSEMELVPSVHPGVRCDGCQMFPINGPRFKCRSCDDFDFCESCFKTRKHNPRHSFGRINEPGQSPTFSGRSGKQLKRHHSSQRGMLIDEWSRVVKNLSVSSSVNQASRLIDCGEQCWQSSGSQGKHWIRLELFPDMLVHKLKMIVDPADSSYMPSLVVVSGGTSLNNLIELKTININPTDTVVSLLNDCTEYHRYIEVAIKQCRSSGIDCKIHGLSAVGRVRAEDEDLATVPFLASDNEEEEDEKTATGSMSRKRLSGLESAATIRTKVFVWGLNDKDQLGGLKGSKIKVPSFSETLSALNVVQVAGGSKSLFAVTAEGKVYACGEATNGRLGLGLSSGTIPIPRQISALSNYVVKKVAVHSGGRHAMALTVDGKVFSWGEGDDGKLGHFSRMNCDKPRLIEALKTKRIRDIACGSSHSAAITSSGELYSWGLGEYGRLGHGDNTTQLRPKLVKVLLGHRVVQVACGSRDAQTLALTDEGMVFSWGDGDFGKLGRGGSEGCNIPQNIERLNGQGVCQIECGAQFSLALTKSGVVWTWGKGDYFRLGHGTDVHVRKPQMVEGLRGKKIVHVAVGALHCLAVTETGQVFAWGDNDHGQQGNGTTTVNRKPTLVQGLEGQKITRVACGSSHSVAWTTVDVTTPSVHEPVLFQTARDSLGASYLGVPSDSDPSLLSNKLNGVNNVKPNRPSLAKILLSLDGNLAKQQALSHVLSSLQIMYARDAVVGALMPSCMMPLECPSSSPVPPSDCSSASSPCEAEGPALPSESEERVSPHPWMDSKRGEVTTSEDATTPSSAVTPSTASASSRPFIPVTDDPGAASIIAETMTKTKEDSENQSKAVGPEPQYLDEFTSLLIPDDTRVMVDLLKLAVSLRSGDKGKEVLSAVLSGMGTACPQVADMLLELCVTELEDVATDCQSGRLSSQPVVVESSHPYIDDTSTSGTVKIPGAEGLRVEFDRQCSTERRHDPLTIMDGANRIVSVRSGREWSDWSSELRIPGDELKWKFTSDGSVNGWGWRFTVYPIMPAAGPKDLLSDRCILSCPSMDLVTCLLDFRLNFASNRSIVPRLAASLAACAQLSALAAGHRMWALQRLRKLLTTEYGQSININRLLGDTEGEARSMSFTGSALAALVKGLPEALQRQYEYEEPIVRGGKQLLHSPFFKVLVALACDLELDTLPCCAETHKWAWFRRYCTASRVSVALDKRSPLPRPFLDEVAKKIRELMADHENMNTLHESHDLFKREQDEQLVQWMNRRPDDWTLSAGGSGTIYGWGHNHRGQLGGIEGAKVKVPTPTEALATLRPVQLIGGEQTLFAVTADGKLYATGYGAGGRLGIGGTESVSTPTLLESIQHVFIRKVAVNSGGKHCLALSSEGELYSWGEAEDGKLGHGNRSPCDRPRVIESLRGVEVVDIAAGGAHSACITASGELYTWGKGRYGRLGHGDSEDQLKPKLVEALQGHRVIDVACGSGDAQTLCLTDDDMVWSWGDGDYGKLGRGGSDGCKVPMKIDSLTGLGVVKVECGSQFSVALTKSGAVYTWGKGDYHRLGHGSDDHVRRPRQVQGLQGKKVIAIATGSLHCVCCTEDGEVYTWGDNDEGQLGDGTTNAIQRPRLVAALQGKKINRVACGSAHTLAWSTSKPTNAGKLPAQVPMEYNHLQEIAIIGLRNRLLLLHHISELFCPCIPMFDLEGRLGETGHGVSVGFDTLRGILISQGKEAAFRKVVQATMVRDRQHGPVVELNRIQVKRSRSKGGLAGPDGTKSVFGQMCAKMSSFSPDSLLLPHRVWKVKFVGESVDDCGGGYSESIAEMCEELQNGLTPLLIVTPNGRDESGANRDCFLLNPAAKSPLHISMFRFLGVLLGIAIRTGSPLSLYLAEPVWKQLAGMNLTIADLSEVDKDFIPGLMYIRDNEASAEEFEAMPLPFTVPSACGQEVQLSSKHSHISLENRSEYVRLAINYRLHEFDEQVSAVREGMARVVPVPLLSLFTGYELETMVCGSPDIPLHLLKSVATYKGVEPTAPLIQWFWEVMESFSNTERSLFLRFVWGRTRLPRTIADFRGRDFVVQVLDKYNPPDHFLPESYTCFFLLKLPRYSCKLVLEEKLKYAIHFCKSIDTDDYARIALSGEPAADDSSDDSDNEDADSFASDSTQDYLTGH

Target ORF information:

RefSeq Version	XM_021477196.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio HECT and RLD domain containing E3 ubiquitin protein ligase 2 (herc2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477196.1

ORF Insert Sequence:

ATGCCTTCTC CATCATTTTC CCTGAATGCC CAGCTGCGCT TCCATGATAA ATGGCTGAAG 
ATCGACTTGC AGCGCGCCTT CTCTCATGAA GGACTGAGCG AGATGTGGAA CGAGATGGTG 
AAGGATGAAG AGATCGTCTT TGACAGAGAA GAGTCTGCCC ATCCAGAGGA CTGTGCTGAT 
TGCACCCAAA AGAAAGATGA CACCAATGAT AAAGAAAAGA AGGAAGAGGA GGAGGCAGCA 
GCAACGGCGG CTTCATCTTT CCATCACTCC ATCATTGAAA TGTGGGACTG GGGGAGGCAG 
CCAGAAACCA GCGAGCTGCG GGACTGTCTG ATGGCTTTGG TAGACGACCA ACAGAAGCTG 
TCTTCTCAGA CGGCTAAGAG CACACTCTCA GCCCTGCGTC TAAGCCAGAG ACTGGTGATC 
CTGGAGCGCT ACCTCATCTC GCTCACTCAC AGCATGATGG AGGAGAAGTA CAAAGTACGG 
TGGAAGAGCC CTCCCAGTCC TCCACTCTCT GCTGTCGACA ACAAAAGCGC TCGGCCAGTT 
AGTAAAGGGG TGGAAGGTTT GGCCAGGGTG GGCTCAAGGG CAGCTCTTTC CTTTGCCTTT 
GCATTTCTCC GCCGAGCCTG GAGATCAGGA GATGATGCAG ACCTGTGCAG TGAGTTACTG 
CAGGAGTCTC TGGATGCTCT GCGGGCTCTT CCAGAAGCTA CTCTGTTTGA TGAGGGCACT 
GTATCTCCTG TTTGGCTGGA GGTGGTTGAG AGGGCTACTA AATTTCTAAG TGACATCCAT 
GGAAGTGCCA GTGGAAAAGC ACCCAGTAAC ATTCCCTTGC AGGATCAGCA CTTGGCCCTT 
GCCATCCTGC TGGAGCTCGC AGTGCAACGA GGAACTCTCA GTCAGCTGCT GTCTGCAGTT 
CTGCTGCTTC TGAGGCTCTG GGACAGTGGA ACTAGAGAGA TGGATAATGA GCGCTCCACC 
CAAGGCACCA GCGCCCCGTT ACTGCCCCTC CTGCAGCGCT TCCAGAACAT CCACAGCAGT 
AAAGAGGAGC CTGTTCCTGA AGAGGAGATT GAGATCCTTT CGGCCCCTCT GAGCCCGAAT 
GAGAGTTTTC TGCGCTACCT TACACTGCCT CAAGATAATG ACCTGGCCAT TGATTTGCGG 
CAGACAGCTG TAGTCATCAT GGCACATTTG GACCGCCTGG CAGCCCCATG CAGCCCCCCG 
CAGTGCAGCT CACCCACTTC TAACAAGGGG ACACTGCAGG AGGTGATTGG TTGGGGTCTG 
CTTGGATGGA AGCCTTATGC TAATGTGAAT GGACCAATTC AGTGCAAGGC ACTATCAAAT 
CTGGGTGTCA CGCAGATTGT CTGCTCAGAG AAGGGTTTCC TCATCCTGAC CATCACTGGT 
GCTGTCTACA CACAAAACTA CAAAAGTACT ACACTAGCAC CTATGCTAGT CCATGCACTG 
ACGTCCAGGA AAATAATGAA GCTTGCAGCT CATCCTGATG GCCAGCATTA TTTGGCTCTT 
TCAGTCAATG GAGAGGTTTT CTCCTGGGGT TGTGGAGATG GAGGCAGGCT AGGACATGGA 
GACACTACGT ATTTAGAGGA GCCCACTATG ATTTCAGTAT TCTCAGGGAG GCCGGTAGGG 
AAGCAGGTTG TACATATTGC CTGTGGCAGC ACATACAGCG CAGCCATCAC AGCAGATGGA 
GAGCTTCACA CATGGGGCCG AGGAAACTAC GGCAGACTGG GCCATGGCTC CAGTGAGGAC 
CAGACCACTC CCATGCTAGT AACTGCACTG AAGGGGCTGA AGGTGATCGA TGTGGCCTGT 
GGAAGCGGAG ATGCTCAGAC GCTTGCTGTG ACTGAGAATG GTCAGGTATG GTCATGGGGT 
GATGGAGATT ATGGGAAACT GGGCCGAGGG GGCAGTGATG GATGCAAAAC ACCAAAACTA 
GTGGAGAAGC TCCAGGATCT GGATATTGTG AAGGTGTGCT GTGGGAGCCA GTTCTCCGTA 
GCGCTCACTA AAGATGGACA AGTTTACACC TGGGGCAAAG GAGACAATCA ACGACTTGGA 
CATGGCACTG ATGAACATGT CCGCTATCCA AAACTACTTG ACAGTCTGCA AGGCAAGAGG 
GTGGTGGACA TAGCGGTTGG CTCCACACAC TGCCTGGCCC TTACAGAAGA AGGAGAGGTG 
CACAGCTGGG GCAGTAATGA CAAACTACAG CACTTTGACA CACTTTTCTC CAATAAGAAG 
CAGCCCAAAG CCTTGCCAGG CCTCAACTCC AAACACATAG TAGGCATCTC CTGCGGTCCT 
GGCCAGAGTT TTTCATGGTC ATCCTGCTCT GAGTGGTCTG TGGGTCTCCG GGTACCCTTC 
GTGGTAGACG TGTGCACTAT GACCTTTGAC CAGCTGGACC TGCTTCTGCG CCAGGTTAGT 
GAGGGCATGG ACGGCAGCTC TGATTGGCCA CCACCACAGG AGAAAGAATG CATGGTCGTG 
GCAGCTCTCA ACCTGCTCAG ACTACAGCTT CATGCAGCCA TCAGTAATCA GGTGGATCCC 
GAGGAGCTGG GTCTGGGTCT GGGCAGTGTG CTGCTTAATA ACCTCAAGCA GACGGTGGTG 
ACTCTGGCCA GCAACGGCGG GGTGCTAAAC ACTGTGCAGG CTGCCGCCCA GACCGTGCTC 
CAGAGTGGCT GGTCAGTGCT ACTCCCCACT GCAGAGGAAC GAGCAAGAGC CCTGTCCTCA 
CTGCTGCCCA ATGCAGCATC TGCTAATGAG ATGAGTGTAA GTCCTGGCCG TCGCTTCATG 
ATCGACCTCT TGGTTAGTAG TCTGATGGCA GATGGAGGGT TGGAGTCTGC TTTGAATGCT 
GCCATCACGG CCGAGATCCA GGATATTGAG GCAAAGAAAG AGGCTCAGAA AGAGAAGGAA 
ATCGATGAGC AGGAAGCCAG CGCCTCTTCT TTGCATCGCT GTCGCACCAC ACTGGACAAA 
GACCTGATCA ACACCGGCAT CTATGAGTCT GCCAACAAAC AGAGCCTCCC ATTGGTCCAG 
CTTGTCCAAC AGCTGCTCAG GAACATTGCT TCACAGACTA TAGCCCGTCT TAAGGATGTG 
GCCCGACGCA TCTCAAATTG TCAAGACACC GAGCAGGTCA GCAAGGAGCG CTCAGCCTCT 
CTGGACCTGC TGCTGCGCTT TCAGAGGCTT TTGGTCTGCA AATTGTATGT CGGAGTCAAT 
GGCACTGTGG GTGTGGGAGG ATACACTTTG AGTCACTCAG AAATTCTGGG AGTTGGCTCT 
CTCCTGAAGA AGTATATGGC TTTGCTTTGC ACACACATCG GTGATATCCT TCCTGTGGCC 
ACCAGCATTG CCCTCAACAG CCATCGTCAC TTTGCAGAGG TGGCTCGTGT CCTTGAGGGA 
GATCTTACAG GTGTTCTGCT ACCAGAGCTC ATTGTGTCCA TTGTGCTGCT GCTGACCCAT 
GATGCTGGAC TAATGCAAGA GACTGGATCC ATTCCTCTGC TCGCTGCTCT TCTGGAGCAC 
TTGGACCGTT TTAACTATCT TGCACCTGGT ACAGAAAGAG ATGACAATGA GGATCTTGCC 
TGGCCTGGAC TCATGGGTTT CTTTTTCAAT GGACCGAGCT CAAAGAACAA CGAGGAGGTG 
TCACTTATCC GCAAAGCAGA CCTGGAGAAT CACAACAAAG ATGGCGGCTT TTGGACTGTA 
ATTGAAGGGA AGGTCTATGA CATAAAGGAC TTTCAAGCTC AGACTCTAAC TGGCAACAGC 
ATCCTTGCCC AGTTTGCAGG AGAAGACCCT GTGGTGGCAT TAGAGGCTGC CCTCCAATTT 
GAAGACACCA GAGAGTCCAT GCAAGCTTTC TGTGTGGGAC AGTATATGGA GCCCAACCAA 
GAAATGGTGA CAACGCCTGA CCTGAGCAGC CTGTCCTCTC CTCTTATAGA TGCTGAGAGG 
AACCTTGGTC TGCTTCTGGG GCTACATGCC TCATATCTGG CTATGAGCAC CCCTCTCTCC 
CGCATAGAGT TAGAATGTGC CAAATGGCTC CAGTCATCTA TTTTCTTCGG TGGACTTCAG 
ACCAGTCAGA TCCACTATAA TTATAACGAG GAGAAGGATG AGGACCACTG CAGCTCTCCA 
GGGACCACCA CACCAGACAA AGCCAAACTG TACTGCCGCA GAATCACCCT GAGTGATCAC 
GCCCAGCCCT TCCTGCAGGC CATCGCTGAC AACACCACCC AGGATCACAC TGTGAAGGAC 
TTTTTATGCC AAATCGAGCG GTGCTGTAAA CAGTACCATC TCATCACTCC CATCACTTTT 
CCACCTGAAC ATCCTGTGGA GGAAGTGGGT CGCCTGCTGC TCTGCTGCCT TCTGAAGCAT 
CAGGATCTTG GTCATGTCGC CCTCTCCCTA GTTGATCAAT GTGCCTTGGG TATGGACCAG 
GGGAAACAGA GGTCCCTCCC GAAATCTGTG ATTGATGTGT GTCGAATGGT GTATCAGGCC 
AAGTGTTCGT TGATAAAAAC ACACCAGGAA CAGGGTCGTT CTTATAAAGA GGTGTGCGCT 
CCTGTTATTG AGAGGTTGCG TTTCCTGTTT AATGAGCTAC GACCTGCCGT CAGTAATGAC 
TTGTCCATTG TGTCCAAACT CAAACTGCTG AGCTCTCAGC CTCGCTGGAA GAGGATCACA 
CAGAAACTGA TCAGAGACCG CCGCAAAAAG AGAGTGCCTA AAAAGTCGGA GTCAGCTGAT 
CTGGAGGAGT GCAAGATGGA AGGGACCAAT GAGGAACCAC CTGCTCCTCT AAGCCCCGCT 
CCTGTTGACA AGAAGCAAAC AACTGTCAAA TCTGCAAAGC AGGATAAGTG GCAGCCTCTG 
CTAAACACCA TCGGCAGTGT TCAGAAGTAC CGCTGGTTAA GGCACAGCGT TCAGGGCTCC 
TTCTCTCAGT CCAGCCTCAT GAGCACCATC GTTGAGTTTT CTTTGAAAGA GGAGCCGCTG 
GATGTGGAGA AGATCAGAAA GTGCCTCCTT AAACAGCTGG AGAGAGCTGA GGTCCGCCTG 
GAGGGTATTG ACACTATGTT GAAGCTGGCA TCTAAAAACT TCCTCCTGCC CTCTGTTCAG 
TATGCCATGC TTTGTGGCTG GCAGAGAGTC ATACCTGAAG GGACCAATAT TGGAGAGCCA 
TTGGCTGACT GTCTAAAGGA TGTAGATCTC ATTCCTCCAT TCAACCGCAT GCTACTAGAG 
GTGACGTTTG GGAAGCTCTA TGCCTGGTCC ATACAGAACG TCCGCAACAT CTTGTTGGAG 
GCTAGTGTCA AGTTCAAGGA ACTGGGTGTG CAACCAGTGC CTCTCCAGAC AATCACTAAT 
GAGAACCCAG CAGGCCCCAG TCTGGGTACC ATCCCTCAGG CACGCTTCCT GCTGGCTATG 
CTCCATATGC TCACTCTCAA ACACGGAGCC AACAGCCTCA GCCTCCTGCT GAACTCTGGC 
ATCCTCGCTC TCACCCAGAG TGTCCTCCGA CTCATTGGTC CTAGCTGTGA TAGCAGTGAG 
GAGGAGCTGA CCTCTGTAGC TCATGGAGGC TCTGCCACAG TTCTGGAAGA GTCCAGAAAG 
GAGACTGCCC CGACCCCTCT GCCCGCTTCC GGCCCTGAGC TGGCAGCTAT GATGAAGATC 
AGCACAAGGG TGATGAGAGG TCCTGACTGG AAGTGGGGGG ACCAGGATGG CCCATCACCA 
GGCCTGGGTC GTGTGATTGG GGAGTTGGGA GAAGATGGTT GGATAAGAGT CCAATGGGAC 
ACCGGCAGCA CCAACTCCTA CAGGATGGGA AAAGAGGGCA AGTATGACCT TAAACTGGCT 
GAGCCGCCAC CTGCCACCCA ACCAGCCACT GAAGACTCTG ACACGGAGGA TGACACAGAA 
GGGGAGGTGA TGGAAAAGTG TGCCCACCCC ACGGCAATCA TGCTGACTAG TACAGTAAAT 
CTGTTGAAGA CACTGTCACT GTCTTCTGGG ATTCATGCAG AGGTGATGCA GGTCGATGCG 
GTCAGTACTC TGTGCGGGCT ACTCAGGATA CTGGTGGAGA GTGGAGCCAA CGACAAGACT 
GGTTGCTCCT CTCACAAGCT GGTGTCCCGA GAGCAGCACA GGAGCTGGTG CACGCTGGGA 
TTTATTCGCA GTATAGCTCT TATGCCTCAG ATGTGCAGTA CCCTGAGCTC CTCTGCCTGG 
ATCAATCTGC TTATACGCAT CGTGGAGGGT CATCAGTCCT TCACTGCAAT CACACTACAG 
AGACAGATTC TTGCCCTGCG GCTTCTACAG GCAGTATTGC CCTCCTGGGA TAAGACTGAA 
CGCTCTCAGG ATATGAAGTT CTTGGTGGAG AAACTGTTTG GGTTCCTCGG CAGCTTGCTC 
AGCACTTGTT CTTCAGATCT GCCTCTTCTT AGGGAAGGTT CAATGCGTAG GAGGAAGTCC 
CGCCCCCAGG CCTCACTGAC AGCCACTCAT AGCAGTACTT TGGCAGAGGA AATAGTGACA 
GTATTGCGCA CGCTTCACTC CCTCAGCCAA TGGAATGCCC TGATTAATGA ATACATTAAC 
TCTCAGCTCA GTGGAATCGG GGATGTCATG GCAGGCCGAC ACACTGAAGT GGCTTTGCTT 
GAAGACTATC TCCCCAATGC GGAAGCAGCA GCGGTGGGCA GTCTGATGGC TGTTTTGGCA 
GTCATCGGGG GGATTGATGG ACGTCTGCGT CTGGGTGGAC GTGTGGTCCA TGAGGAGTAT 
GGAGAAGGCA CAGTGACTCG CATCACACCT AAAGGACGAA TCACTGTTCA GTTTCATGAG 
ATGAGGAACT GCAAAGTGTG CCTGCTCAGT CAGCTCAAAC CGCTTCACAT GATTCCATTC 
AGCGTTCAGA ATCTGCCCTT CGCTGAGCCC ATGCTAATGG TGTGGGCCCA GCTAGTCAAC 
TTGGCTGGGA GCAAGCTGGA GAAACAACGC ATGAAGAAAT CTCTTAGTAG AGGACTTACA 
GCGGATCAGG TAGATGTACA TTTGTTGCGC TGTCAGCAGC TCAGGCTGTA CATCTTGAAG 
GCTGCACGAG CTCTACTCTC CCATCAGGAC AAACTCAGAC AGATCCTTTC TCAGGCGGCC 
GTGGTTGATG TCAGCCCCAA TCCTACAGAG GATCAGTGCG CGTCGTCTCC TGATGTAGGA 
GATTTGTCTC CTGAGGGTCC TCAGCCTCCT CTCATCCTCC TTCAGCAGTT GCTGTCAGCA 
TCCACTCAAC CTTCACCCAT CAAAGCCATA TTCCACCGCC AGGAGTTAGA GGCAGCGGCT 
CTGGCTGTGT GTCAGTACCT GGCGGTTGAA TCCACACACC CGTCATCGCC TCTGTTTGAG 
GACAGCAGCT CTAGCGAAGC CACTACACCA GTCACTGTGC AGCACATGCG TCCACCCAAA 
CAAAAGAAAC ACAAGACGTC ACCTGTGCCC CCTGCTCCCA TCGTCCTGCA GCTGATGGAG 
ATGGGCTTTC AAAGGAAAAA CATCGAGTTT GCTCTTAAAT CACTGTCTGG AACCACAGGT 
GCTTCTGGAG TGCCAGGTGT CGAGGCATTG GTGGGCTGGT TGTTGGACCA TCCAGATGTT 
CTCATTACAG ATCTGTCGGA TGCAGAGACT GTATCAGATG AATACTCTGA TGAAGAGGTG 
CTGGAAGAGC TTGAGGAGCC TGAGCCTGCC TTTCCTGTGC CTCCTGGGGT TGTGGTGACT 
GAGAGTCAGA CGTTTAAAAA GAGGTCGGAC TTTCAGAGCA ATGACGATTA TGCTGTTTAC 
GTGAGAGAGA ACATTCAGGT GGGCATGATG GTACGGTGTT GCAGGACATA TGAGGAAGTG 
TATGAAGGAG ATGTGGGGAA AGTGATCAAG CTTGACAGAG ACGGCCTGCA TGACCTGAAT 
GTTCAGTGTG ACTGGCAGCA GAAAGGAGGA ACTTACTGGG TCCGTTACAT CCACACTGAG 
CTGCTGGGGT TTCCCCCTCA GAGTTCTCCA TCTCATATAA AGATTGGTGA TAAAGTGCGA 
GTGAAGCCCT CGGTCACAAC ACCAAAATAC AAGTGGGGGT CAGTCACTCA TAGGAGTGTG 
GGTGTGGTCA AAGCCTTCAG TGCCAATGGG AAGGATGTCA TTGTGGACTT TCCTCAGCAG 
TCGCACTGGA CTGGACTGCT GTCAGAGATG GAGCTTGTGC CCAGCGTACA TCCTGGTGTC 
AGGTGTGACG GATGTCAGAT GTTCCCTATA AATGGCCCAA GGTTTAAGTG TCGGAGCTGT 
GATGACTTTG ACTTTTGTGA GAGCTGCTTC AAGACCCGTA AACACAATCC TAGGCATTCA 
TTTGGGAGGA TAAACGAACC AGGTCAGTCA CCCACCTTTA GTGGGCGCTC AGGAAAGCAG 
CTGAAAAGAC ATCACAGCAG TCAGCGTGGC ATGCTGATCG ACGAGTGGTC AAGAGTGGTG 
AAGAACCTCA GCGTATCGTC TTCTGTTAAC CAGGCGTCTC GACTCATCGA CTGTGGAGAG 
CAATGCTGGC AGTCCTCAGG CTCTCAGGGC AAGCACTGGA TCAGATTGGA GCTTTTCCCA 
GACATGTTAG TCCACAAACT GAAAATGATT GTAGATCCAG CTGACAGCAG CTACATGCCT 
TCATTGGTGG TGGTATCAGG TGGAACCTCA CTCAACAATT TGATCGAGTT GAAGACCATC 
AACATCAATC CCACTGACAC TGTCGTCTCT CTACTCAATG ACTGCACAGA GTATCACAGG 
TACATTGAGG TTGCTATAAA GCAGTGTCGA AGTTCAGGTA TTGACTGTAA GATTCATGGC 
TTGAGTGCTG TGGGACGTGT CCGTGCCGAG GATGAAGATC TTGCAACTGT ACCTTTCCTG 
GCATCTGACA ACGAGGAAGA GGAAGATGAG AAGACCGCGA CTGGAAGTAT GTCTCGAAAG 
AGATTGTCAG GCCTCGAGTC TGCAGCCACT ATCAGAACCA AAGTCTTTGT CTGGGGTCTT 
AATGATAAGG ACCAACTTGG AGGACTTAAG GGGTCCAAGA TCAAAGTCCC TTCATTCTCA 
GAGACTCTGT CTGCTCTGAA TGTAGTGCAG GTGGCTGGTG GCTCTAAAAG TCTTTTTGCT 
GTGACTGCTG AAGGTAAAGT ATATGCCTGT GGAGAGGCCA CCAATGGAAG ACTTGGTTTA 
GGCCTCTCCA GTGGAACCAT CCCAATTCCC AGACAGATAT CAGCTCTCAG CAACTATGTG 
GTCAAGAAGG TTGCTGTGCA TTCAGGTGGA CGGCATGCCA TGGCACTTAC CGTGGATGGG 
AAAGTATTCT CTTGGGGTGA AGGAGACGAT GGCAAGCTTG GACACTTCAG CCGAATGAAC 
TGTGATAAGC CAAGGCTGAT CGAGGCATTG AAAACCAAAC GCATTCGAGA TATTGCGTGT 
GGCAGCTCTC ACAGTGCCGC CATCACCTCC AGTGGAGAGC TGTACAGCTG GGGTCTGGGG 
GAATATGGCC GTCTGGGCCA TGGGGACAAC ACCACCCAAC TCAGACCCAA ACTGGTCAAG 
GTGCTTTTAG GCCATCGCGT TGTCCAGGTA GCTTGTGGAA GTAGAGATGC CCAAACTCTT 
GCACTGACAG ATGAAGGAAT GGTGTTCTCA TGGGGAGATG GAGACTTTGG GAAACTGGGC 
CGTGGTGGCA GCGAGGGATG TAACATTCCT CAAAACATTG AGCGTCTGAA TGGACAGGGG 
GTCTGTCAAA TTGAATGTGG AGCTCAATTC TCCCTCGCCC TCACCAAATC TGGAGTTGTC 
TGGACCTGGG GTAAAGGGGA TTACTTCAGA CTGGGTCACG GCACAGATGT TCATGTCCGC 
AAACCCCAGA TGGTTGAAGG ACTTAGGGGG AAGAAGATTG TTCATGTGGC CGTTGGTGCT 
CTGCACTGCC TGGCTGTCAC CGAGACTGGA CAGGTGTTCG CGTGGGGGGA TAATGACCAT 
GGGCAGCAGG GGAATGGCAC AACCACAGTC AACAGGAAGC CCACATTGGT GCAGGGTCTA 
GAGGGTCAGA AAATTACTCG TGTGGCCTGC GGTTCTTCTC ATAGTGTTGC CTGGACCACA 
GTAGATGTCA CTACACCCTC CGTCCATGAG CCTGTGCTCT TCCAGACTGC TAGAGACTCT 
CTGGGAGCCT CTTACCTTGG TGTGCCATCA GATAGTGACC CGTCTTTACT GAGCAATAAG 
CTAAATGGCG TAAACAATGT GAAGCCCAAC AGGCCATCTC TGGCAAAGAT CCTTCTCTCT 
CTGGATGGTA ATCTGGCCAA ACAGCAGGCC CTATCCCATG TGCTGTCTTC CCTGCAGATC 
ATGTATGCCA GAGATGCAGT GGTGGGTGCA TTAATGCCAT CCTGTATGAT GCCTCTGGAG 
TGTCCCTCCA GCTCTCCGGT GCCCCCATCA GACTGTTCAT CAGCTTCCAG CCCCTGTGAG 
GCTGAGGGTC CTGCCCTGCC CTCAGAGAGT GAGGAGAGGG TCAGTCCGCA CCCCTGGATG 
GACAGCAAAC GAGGAGAGGT AACCACGTCT GAGGATGCCA CTACCCCATC TTCAGCAGTG 
ACCCCTTCTA CTGCCTCTGC CTCATCTCGA CCCTTCATAC CAGTCACAGA TGATCCTGGT 
GCTGCCAGCA TCATTGCTGA GACCATGACC AAAACCAAAG AGGACTCTGA GAACCAGAGT 
AAAGCCGTGG GTCCAGAGCC ACAGTATCTG GATGAGTTCA CCAGTCTCCT GATCCCTGAT 
GACACGCGTG TGATGGTGGA CCTGTTAAAA CTAGCTGTAT CTCTGCGCTC TGGTGATAAA 
GGAAAGGAAG TGCTGTCTGC TGTTCTGTCT GGCATGGGTA CAGCCTGCCC ACAGGTGGCG 
GATATGCTTC TGGAGCTGTG TGTGACTGAG TTGGAGGACG TGGCGACTGA CTGTCAGAGT 
GGACGGCTTT CCTCTCAACC TGTGGTGGTG GAAAGCAGCC ATCCATATAT TGACGATACA 
TCCACCAGCG GAACTGTCAA AATACCAGGT GCTGAGGGTT TGCGGGTTGA GTTTGATCGC 
CAGTGCTCAA CAGAAAGGAG ACATGACCCT CTTACCATCA TGGATGGAGC CAATAGAATT 
GTGTCTGTTC GATCAGGTCG TGAGTGGTCT GATTGGTCCA GTGAATTGAG AATTCCAGGA 
GACGAGCTCA AATGGAAGTT CACCAGTGAC GGCTCTGTGA ACGGCTGGGG CTGGCGCTTC 
ACAGTCTATC CCATCATGCC TGCGGCTGGT CCTAAAGATC TTCTGTCAGA CCGCTGCATT 
TTGTCTTGCC CTTCAATGGA TCTAGTAACA TGTCTGCTAG ACTTTCGTCT GAACTTTGCC 
TCAAACCGCA GCATTGTGCC CAGATTAGCA GCCTCACTTG CTGCTTGTGC GCAGCTCAGC 
GCCTTGGCGG CTGGCCATCG TATGTGGGCT CTGCAGAGGT TACGCAAGCT CTTAACTACT 
GAATACGGCC AATCCATTAA CATCAACCGC CTCTTAGGAG ACACCGAAGG AGAGGCTCGC 
TCTATGAGTT TTACAGGAAG TGCATTAGCT GCTCTGGTGA AAGGACTTCC TGAGGCATTA 
CAGAGACAGT ATGAGTACGA GGAGCCCATA GTTAGGGGTG GCAAACAGTT ACTCCACAGC 
CCGTTCTTCA AGGTGTTGGT GGCGCTGGCG TGTGATCTGG AGTTGGACAC TCTTCCATGT 
TGTGCTGAGA CACACAAGTG GGCGTGGTTT CGACGCTATT GCACCGCCTC CAGAGTGTCA 
GTGGCTCTGG ACAAGAGGAG CCCTTTGCCC CGCCCTTTTC TGGATGAGGT GGCAAAGAAA 
ATCCGTGAGC TGATGGCAGA TCATGAGAAC ATGAACACGC TTCATGAGAG CCATGACTTG 
TTTAAGAGGG AGCAGGATGA GCAGTTGGTT CAGTGGATGA ACAGACGACC AGATGATTGG 
ACACTCTCTG CGGGTGGCAG TGGTACAATA TACGGCTGGG GGCACAATCA CCGGGGTCAG 
TTAGGTGGCA TTGAAGGAGC CAAAGTAAAG GTTCCCACAC CAACAGAAGC TCTAGCCACA 
CTGCGGCCAG TTCAGCTTAT TGGAGGAGAG CAAACACTGT TTGCCGTCAC TGCTGATGGG 
AAGCTGTATG CCACAGGATA TGGTGCCGGT GGTCGACTGG GAATAGGAGG GACAGAGTCC 
GTCTCCACTC CGACCCTGCT GGAGTCCATC CAGCATGTTT TCATCAGAAA GGTGGCTGTG 
AACTCTGGAG GGAAACACTG TTTGGCTCTT TCCTCTGAAG GAGAGCTGTA CTCCTGGGGG 
GAGGCCGAAG ATGGAAAACT GGGCCATGGA AACAGAAGCC CATGTGATCG TCCACGCGTG 
ATTGAGTCTC TCAGAGGGGT GGAAGTGGTG GACATTGCTG CCGGTGGGGC TCACAGTGCC 
TGCATTACTG CCAGTGGAGA GCTCTACACA TGGGGCAAAG GTCGCTATGG GCGCCTGGGT 
CATGGAGACA GCGAAGACCA ACTTAAACCA AAACTGGTGG AGGCTCTTCA GGGTCACCGG 
GTGATAGATG TGGCATGTGG CAGTGGAGAT GCACAGACCC TCTGTCTGAC TGATGATGAC 
ATGGTATGGT CGTGGGGCGA TGGAGACTAT GGCAAATTGG GCCGAGGAGG TAGTGATGGA 
TGTAAAGTGC CGATGAAGAT CGATTCATTA ACCGGATTGG GTGTTGTGAA AGTGGAATGT 
GGCTCTCAGT TTTCTGTGGC CCTTACCAAG TCTGGAGCAG TTTACACATG GGGGAAGGGT 
GACTATCACA GACTGGGACA CGGCTCAGAT GATCATGTTC GCAGACCCCG GCAGGTCCAG 
GGTTTACAAG GCAAAAAGGT CATCGCAATA GCAACTGGTT CCCTTCACTG CGTTTGCTGC 
ACGGAGGACG GGGAAGTGTA CACATGGGGT GACAATGATG AGGGTCAGCT TGGGGACGGT 
ACGACCAATG CTATTCAGAG ACCCAGGCTG GTGGCAGCGC TGCAGGGCAA GAAGATTAAT 
CGTGTGGCCT GTGGTTCAGC ACATACACTT GCCTGGTCCA CTAGTAAACC AACCAATGCT 
GGAAAACTCC CTGCTCAGGT TCCAATGGAG TACAACCACC TGCAAGAAAT CGCCATCATC 
GGCCTGAGGA ATCGGTTACT GCTACTGCAC CACATCTCTG AGCTCTTCTG CCCCTGCATC 
CCAATGTTTG ACTTAGAAGG ACGCCTGGGT GAAACTGGAC ATGGTGTGTC TGTTGGCTTT 
GACACGCTCC GTGGAATCCT CATATCACAG GGCAAGGAGG CAGCATTCCG CAAAGTGGTG 
CAGGCTACCA TGGTCAGAGA CAGGCAGCAT GGGCCAGTGG TTGAGCTGAA CAGGATACAG 
GTCAAGCGTT CACGTAGTAA AGGAGGTCTG GCAGGGCCGG ATGGCACCAA GTCAGTATTT 
GGGCAGATGT GTGCTAAGAT GAGTTCTTTC AGTCCAGACA GTCTGCTGCT GCCACACCGC 
GTCTGGAAGG TCAAGTTTGT GGGTGAGTCG GTGGATGACT GTGGTGGCGG ATACAGTGAA 
TCTATCGCAG AAATGTGCGA GGAGCTTCAG AATGGCCTCA CTCCTCTGCT CATCGTCACT 
CCTAATGGCA GAGACGAGTC AGGAGCGAAC AGAGATTGCT TTCTGCTCAA CCCAGCTGCC 
AAATCCCCTC TGCACATCAG CATGTTCCGC TTCCTAGGGG TGCTCCTGGG CATAGCGATC 
CGAACAGGAA GTCCTCTTAG TCTGTATCTG GCCGAACCTG TGTGGAAACA GCTGGCAGGC 
ATGAACCTGA CCATTGCTGA TCTCAGTGAG GTGGACAAGG ACTTCATCCC CGGTCTGATG 
TATATTAGAG ATAACGAGGC GTCTGCGGAG GAGTTTGAGG CGATGCCTCT TCCCTTCACT 
GTGCCCAGTG CCTGCGGACA AGAAGTCCAG CTCAGCTCCA AACATTCCCA CATCAGCCTT 
GAAAACCGCA GTGAATATGT GCGGCTAGCA ATAAATTACA GGCTGCATGA GTTTGATGAG 
CAGGTGTCTG CTGTAAGAGA AGGTATGGCG CGTGTGGTTC CGGTACCGCT CCTCTCTCTA 
TTTACCGGCT ATGAGCTGGA GACCATGGTG TGCGGAAGTC CGGACATCCC TCTGCATCTG 
CTCAAATCAG TGGCCACATA TAAAGGAGTA GAGCCCACTG CACCGCTCAT CCAGTGGTTC 
TGGGAGGTGA TGGAGTCTTT CTCTAACACA GAACGCTCGC TCTTCCTCAG GTTCGTCTGG 
GGCCGCACGC GTCTTCCCAG GACCATCGCA GACTTCCGTG GACGAGACTT TGTAGTGCAG 
GTGCTGGATA AATATAACCC TCCCGACCAC TTCCTCCCCG AGTCCTACAC CTGCTTCTTC 
CTGCTCAAGC TGCCTCGCTA CTCCTGCAAG CTAGTCTTGG AGGAGAAACT CAAATACGCC 
ATTCACTTCT GCAAGTCCAT CGACACTGAT GACTACGCAC GGATCGCTCT GAGCGGAGAA 
CCGGCCGCAG ACGACAGCAG TGATGACTCG GACAATGAAG ACGCAGACTC ATTCGCTTCT 
GACTCCACAC AGGATTACCT GACAGGCCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC2 ( XM_017356648.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017212137.1 Danio rerio HECT and RLD domain containing E3 ubiquitin protein ligase 2 (herc2), transcript variant X1, mRNA.

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CloneID	ODa59481
Clone ID Related Accession (Same CDS sequence)	XM_017356648.1
Accession Version	XM_017356648.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 14502bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase HERC2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTCTC CATCATTTTC CCTGAATGCC CAGCTGCGCT TCCATGATAA ATGGCTGAAG 
ATCGACTTGC AGCGCGCCTT CTCTCATGAA GGACTGAGCG AGATGTGGAA CGAGATGGTG 
AAGGATGAAG AGATCGTCTT TGACAGAGAA GAGTCTGCCC ATCCAGAGGA CTGTGCTGAT 
TGCACCCAAA AGAAAGATGA CACCAATGAT AAAGAAAAGA AGGAAGAGGA GGAGGCAGCA 
GCAACGGCGG CTTCATCTTT CCATCACTCC ATCATTGAAA TGTGGGACTG GGGGAGGCAG 
CCAGAAACCA GCGAGCTGCG GGACTGTCTG ATGGCTTTGG TAGACGACCA ACAGAAGCTG 
TCTTCTCAGA CGGCTAAGAG CACACTCTCA GCCCTGCGTC TAAGCCAGAG ACTGGTGATC 
CTGGAGCGCT ACCTCATCTC GCTCACTCAC AGCATGATGG AGGAGAAGTA CAAAGTACGG 
TGGAAGAGCC CTCCCAGTCC TCCACTCTCT GCTGTCGACA ACAAAAGCGC TCGGCCAGTT 
AGTAAAGGGG TGGAAGGTTT GGCCAGGGTG GGCTCAAGGG CAGCTCTTTC CTTTGCCTTT 
GCATTTCTCC GCCGAGCCTG GAGATCAGGA GATGATGCAG ACCTGTGCAG TGAGTTACTG 
CAGGAGTCTC TGGATGCTCT GCGGGCTCTT CCAGAAGCTA CTCTGTTTGA TGAGGGCACT 
GTATCTCCTG TTTGGCTGGA GGTGGTTGAG AGGGCTACTA AATTTCTAAG TGACATCCAT 
GGAAGTGCCA GTGGAAAAGC ACCCAGTAAC ATTCCCTTGC AGGATCAGCA CTTGGCCCTT 
GCCATCCTGC TGGAGCTCGC AGTGCAACGA GGAACTCTCA GTCAGCTGCT GTCTGCAGTT 
CTGCTGCTTC TGAGGCTCTG GGACAGTGGA ACTAGAGAGA TGGATAATGA GCGCTCCACC 
CAAGGCACCA GCGCCCCGTT ACTGCCCCTC CTGCAGCGCT TCCAGAACAT CCACAGCAGT 
AAAGAGGAGC CTGTTCCTGA AGAGGAGATT GAGATCCTTT CGGCCCCTCT GAGCCCGAAT 
GAGAGTTTTC TGCGCTACCT TACACTGCCT CAAGATAATG ACCTGGCCAT TGATTTGCGG 
CAGACAGCTG TAGTCATCAT GGCACATTTG GACCGCCTGG CAGCCCCATG CAGCCCCCCG 
CAGTGCAGCT CACCCACTTC TAACAAGGGG ACACTGCAGG AGGTGATTGG TTGGGGTCTG 
CTTGGATGGA AGCCTTATGC TAATGTGAAT GGACCAATTC AGTGCAAGGC ACTATCAAAT 
CTGGGTGTCA CGCAGATTGT CTGCTCAGAG AAGGGTTTCC TCATCCTGAC CATCACTGGT 
GCTGTCTACA CACAAAACTA CAAAAGTACT ACACTAGCAC CTATGCTAGT CCATGCACTG 
ACGTCCAGGA AAATAATGAA GCTTGCAGCT CATCCTGATG GCCAGCATTA TTTGGCTCTT 
TCAGTCAATG GAGAGGTTTT CTCCTGGGGT TGTGGAGATG GAGGCAGGCT AGGACATGGA 
GACACTACGT ATTTAGAGGA GCCCACTATG ATTTCAGTAT TCTCAGGGAG GCCGGTAGGG 
AAGCAGGTTG TACATATTGC CTGTGGCAGC ACATACAGCG CAGCCATCAC AGCAGATGGA 
GAGCTTCACA CATGGGGCCG AGGAAACTAC GGCAGACTGG GCCATGGCTC CAGTGAGGAC 
CAGACCACTC CCATGCTAGT AACTGCACTG AAGGGGCTGA AGGTGATCGA TGTGGCCTGT 
GGAAGCGGAG ATGCTCAGAC GCTTGCTGTG ACTGAGAATG GTCAGGTATG GTCATGGGGT 
GATGGAGATT ATGGGAAACT GGGCCGAGGG GGCAGTGATG GATGCAAAAC ACCAAAACTA 
GTGGAGAAGC TCCAGGATCT GGATATTGTG AAGGTGTGCT GTGGGAGCCA GTTCTCCGTA 
GCGCTCACTA AAGATGGACA AGTTTACACC TGGGGCAAAG GAGACAATCA ACGACTTGGA 
CATGGCACTG ATGAACATGT CCGCTATCCA AAACTACTTG ACAGTCTGCA AGGCAAGAGG 
GTGGTGGACA TAGCGGTTGG CTCCACACAC TGCCTGGCCC TTACAGAAGA AGGAGAGGTG 
CACAGCTGGG GCAGTAATGA CAAACTACAG CACTTTGACA CACTTTTCTC CAATAAGAAG 
CAGCCCAAAG CCTTGCCAGG CCTCAACTCC AAACACATAG TAGGCATCTC CTGCGGTCCT 
GGCCAGAGTT TTTCATGGTC ATCCTGCTCT GAGTGGTCTG TGGGTCTCCG GGTACCCTTC 
GTGGTAGACG TGTGCACTAT GACCTTTGAC CAGCTGGACC TGCTTCTGCG CCAGGTTAGT 
GAGGGCATGG ACGGCAGCTC TGATTGGCCA CCACCACAGG AGAAAGAATG CATGGTCGTG 
GCAGCTCTCA ACCTGCTCAG ACTACAGCTT CATGCAGCCA TCAGTAATCA GGTGGATCCC 
GAGGAGCTGG GTCTGGGTCT GGGCAGTGTG CTGCTTAATA ACCTCAAGCA GACGGTGGTG 
ACTCTGGCCA GCAACGGCGG GGTGCTAAAC ACTGTGCAGG CTGCCGCCCA GACCGTGCTC 
CAGAGTGGCT GGTCAGTGCT ACTCCCCACT GCAGAGGAAC GAGCAAGAGC CCTGTCCTCG 
CTGCTGCCCA ATGCAGCATC TGCTAATGAG ATGAGTGTAA GTCCTGGCCG TCGCTTCATG 
ATCGACCTCT TGGTTAGTAG TCTGATGGCA GATGGAGGGT TGGAGTCTGC TTTGAATGCT 
GCCATCACGG CCGAGATCCA GGATATTGAG GCAAAGAAAG AGGCTCAGAA AGAGAAGGAA 
ATCGATGAGC AGGAAGCCAG CGCCTCTTCT TTGCATCGCT GTCGCACCAC ACTGGACAAA 
GACCTGATCA ACACCGGCAT CTATGAGTCT GCCAACAAAC AGAGCCTCCC ATTGGTCCAG 
CTTGTCCAAC AGCTGCTCAG GAACATTGCT TCACAGACTA TAGCCCGTCT TAAGGATGTG 
GCCCGACGCA TCTCAAATTG TCAAGACACC GAGCAGGTCA GCAAGGAGCG CTCAGCCTCT 
CTGGACCTGC TGCTGCGCTT TCAGAGGCTT TTGGTCTGCA AATTGTATGT CGGAGTCAAT 
GGCACTGTGG GTGTGGGAGG ATACACTTTG AGTCACTCAG AAATTCTGGG AGTTGGCTCT 
CTCCTGAAGA AGTATATGGC TTTGCTTTGC ACACACATCG GTGATATCCT TCCTGTGGCC 
ACCAGCATTG CCCTCAACAG CCATCGTCAC TTTGCAGAGG TGGCTCGTGT CCTTGAGGGA 
GATCTTACAG GTGTTCTGCT ACCAGAGCTC ATTGTGTCCA TTGTGCTGCT GCTGACCCAT 
GATGCTGGAC TAATGCAAGA GACTGGATCC ATTCCTCTGC TCGCTGCTCT TCTGGAGCAC 
TTGGACCGTT TTAACTATCT TGCACCTGGT ACAGAAAGAG ATGACAATGA GGATCTTGCC 
TGGCCTGGAC TCATGGGTTT CTTTTTCAAT GGACCGAGCT CAAAGAACAA CGAGGAGGTG 
TCACTTATCC GCAAAGCAGA CCTGGAGAAT CACAACAAAG ATGGCGGCTT TTGGACTGTA 
ATTGAAGGGA AGGTCTATGA CATAAAGGAC TTTCAAGCTC AGACTCTAAC TGGCAACAGC 
ATCCTTGCCC AGTTTGCAGG AGAAGACCCT GTGGTGGCAT TAGAGGCTGC CCTCCAATTT 
GAAGACACCA GAGAGTCCAT GCAAGCTTTC TGTGTGGGAC AGTATATGGA GCCCAACCAA 
GAAATGGTGA CAACGCCTGA CCTGAGCAGC CTGTCCTCTC CTCTTATAGA TGCTGAGAGG 
AACCTTGGTC TGCTTCTGGG GCTACATGCC TCATATCTGG CTATGAGCAC CCCTCTCTCC 
CGCATAGAGT TAGAATGTGC CAAATGGCTC CAGTCATCTA TTTTCTTCGG TGGACTTCAG 
ACCAGTCAGA TCCACTATAA TTATAACGAG GAGAAGGATG AGGACCACTG CAGCTCTCCA 
GGGACCACCA CACCAGACAA AGCCAAACTG TACTGCCGCA GAATCACCCT GAGTGATCAC 
GCCCAGCCCT TCCTGCAGGC CATCGCTGAC AACACCACCC AGGATCACAC TGTGAAGGAC 
TTTTTATGCC AAATCGAGCG GTGCTGTAAA CAGTACCATC TCATCACTCC CATCACTTTT 
CCACCTGAAC ATCCTGTGGA GGAAGTGGGT CGCCTGCTGC TCTGCTGCCT TCTGAAGCAT 
CAGGATCTTG GTCATGTCGC CCTCTCCCTA GTTGATCAAT GTGCCTTGGG TATGGACCAG 
GGGAAACAGA GGTCCCTCCC GAAATCTGTG ATTGATGTGT GTCGAATGGT GTATCAGGCC 
AAGTGTTCGT TGATAAAAAC ACACCAGGAA CAGGGTCGTT CTTATAAAGA GGTGTGCGCT 
CCTGTTATTG AGAGGTTGCG TTTCCTGTTT AATGAGCTAC GACCTGCCGT CAGTAATGAC 
TTGTCCATTG TGTCCAAACT CAAACTGCTG AGCTCTCAGC CTCGCTGGAA GAGGATCACA 
CAGAAACTGA TCAGAGACCG CCGCAAAAAG AGAGTGCCTA AAAAGTCGGA GTCAGCTGAT 
CTGGAGGAGT GCAAGATGGA GAGGGAAGGG ACCAATGAGG AACCACCTGC TCCTCTAAGC 
CCCGCTCCTG TTGACAAGAA GCAAACAACT GTCAAATCTG CAAAGCAGGA TAAGTGGCAG 
CCTCTGCTAA ACACCATCGG CAGTGTTCAG AAGTACCGCT GGTTAAGGCA CAGCGTTCAG 
GGCTCCTTCT CTCAGTCCAG CCTCATGAGC ACCATCGTTG AGTTTTCTTT GAAAGAGGAG 
CCGCTGGATG TGGAGAAGAT CAGAAAGTGC CTCCTTAAAC AGCTGGAGAG AGCTGAGGTC 
CGCCTGGAGG GTATTGACAC TATGTTGAAG CTGGCATCTA AAAACTTCCT CCTGCCCTCT 
GTTCAGTATG CCATGCTTTG TGGCTGGCAG AGAGTCATAC CTGAAGGGAC CAATATTGGA 
GAGCCATTGG CTGACTGTCT AAAGGATGTA GATCTCATTC CTCCATTCAA CCGCATGCTA 
CTAGAGGTGA CGTTTGGGAA GCTCTATGCC TGGTCCATAC AGAACGTCCG CAACATCTTG 
TTGGAGGCTA GTGTCAAGTT CAAGGAACTG GGTGTGCAAC CAGTGCCTCT CCAGACAATC 
ACTAATGAGA ACCCAGCAGG CCCCAGTCTG GGTACCATCC CTCAGGCACG CTTCCTGCTG 
GCTATGCTCC ATATGCTCAC TCTCAAACAC GGAGCCAACA GCCTCAGCCT CCTGCTGAAC 
TCTGGCATCC TCGCTCTCAC CCAGAGTGTC CTCCGACTCA TTGGTCCTAG CTGTGATAGC 
AGTGAGGAGG AGCTGACCTC TGTAGCTCAT GGAGGCTCTG CCACAGTTCT GGAAGAGTCC 
AGAAAGGAGA CTGCCCCGAC CCCTCTGCCC GCTTCCGGCC CTGAGCTGGC AGCTATGATG 
AAGATCAGCA CAAGGGTGAT GAGAGGTCCT GACTGGAAGT GGGGGGACCA GGATGGCCCA 
TCACCAGGCC TGGGTCGTGT GATTGGGGAG TTGGGAGAAG ATGGTTGGAT AAGAGTCCAA 
TGGGACACCG GCAGCACCAA CTCCTACAGG ATGGGAAAAG AGGGCAAGTA TGACCTTAAA 
CTGGCTGAGC CGCCACCTGC CACCCAACCA GCCACTGAAG ACTCTGACAC GGAGGATGAC 
ACAGAAGGGG AGGTGATGGA AAAGTGTGCC CACCCCACGG CAATCATGCT GACTAGTACA 
GTAAATCTGT TGAAGACACT GTCACTGTCT TCTGGGATTC ATGCAGAGGT GATGCAGGTC 
GATGCGGTCA GTACTCTGTG CGGGCTACTC AGGATACTGG TGGAGAGTGG AGCCAACGAC 
AAGACTGGTT GCTCCTCTCA CAAGCTGGTG TCCCGAGAGC AGCACAGGAG CTGGTGCACG 
CTGGGATTTA TTCGCAGTAT AGCTCTTATG CCTCAGATGT GCAGTACCCT GAGCTCCTCT 
GCCTGGATCA ATCTGCTTAT ACGCATCGTG GAGGGTCATC AGTCCTTCAC TGCAATCACA 
CTACAGAGAC AGATTCTTGC CCTGCGGCTT CTACAGGCAG TATTGCCCTC CTGGGATAAG 
ACTGAACGCT CTCAGGATAT GAAGTTCTTG GTGGAGAAAC TGTTTGGGTT CCTCGGCAGC 
TTGCTCAGCA CTTGTTCTTC AGATCTGCCT CTTCTTAGGG AAGGTTCAAT GCGTAGGAGG 
AAGTCCCGCC CCCAGGCCTC ACTGACAGCC ACTCATAGCA GTACTTTGGC AGAGGAAATA 
GTGACAGTAT TGCGCACGCT TCACTCCCTC AGCCAATGGA ATGCCCTGAT TAATGAATAC 
ATTAACTCTC AGCTCAGTGG AATCGGGGAT GTCATGGCAG GCCGACACAC TGAAGTGGCT 
TTGCTTGAAG ACTATCTCCC CAATGCGGAA GCAGCAGCGG TGGGCAGTCT GATGGCTGTT 
TTGGCAGTCA TCGGGGGGAT TGATGGACGT CTGCGTCTGG GTGGACGTGT GGTCCATGAG 
GAGTATGGAG AAGGCACAGT GACTCGCATC ACACCTAAAG GACGAATCAC TGTTCAGTTT 
CATGAGATGA GGAACTGCAA AGTGTGCCTG CTCAGTCAGC TCAAACCGCT TCACATGATT 
CCATTCAGCG TTCAGAATCT GCCCTTCGCT GAGCCCATGC TAATGGTGTG GGCCCAGCTA 
GTCAACTTGG CTGGGAGCAA GCTGGAGAAA CAACGCATGA AGAAATCTCT TAGTAGAGGA 
CTTACAGCGG ATCAGGTAGA TGTACATTTG TTGCGCTGTC AGCAGCTCAG GCTGTACATC 
TTGAAGGCTG CACGAGCTCT ACTCTCCCAT CAGGACAAAC TCAGACAGAT CCTTTCTCAG 
GCGGCCGTGG TTGATGTCAG CCCCAATCCT ACAGAGGATC AGTGCGCGTC GTCTCCTGAT 
GTAGGAGATT TGTCTCCTGA GGGTCCTCAG CCTCCTCTCA TCCTCCTTCA GCAGTTGCTG 
TCAGCATCCA CTCAACCTTC ACCCATCAAA GCCATATTCC ACCGCCAGGA GTTAGAGGCA 
GCGGCTCTGG CTGTGTGTCA GTACCTGGCG GTTGAATCCA CACACCCGTC ATCGCCTCTG 
TTTGAGGACA GCAGCTCTAG CGAAGCCACT ACACCAGTCA CTGTGCAGCA CATGCGTCCA 
CCCAAACAAA AGAAACACAA GACGTCACCT GTGCCCCCTG CTCCCATCGT CCTGCAGCTG 
ATGGAGATGG GCTTTCAAAG GAAAAACATC GAGTTTGCTC TTAAATCACT GTCTGGAACC 
ACAGGTGCTT CTGGAGTGCC AGGTGTCGAG GCATTGGTGG GCTGGTTGTT GGACCATCCA 
GATGTTCTCA TTACAGATCT GTCGGATGCA GAGACTGTAT CAGATGAATA CTCTGATGAA 
GAGGTGCTGG AAGAGCTTGA GGAGCCTGAG CCTGCCTTTC CTGTGCCTCC TGGGGTTGTG 
GTGACTGAGA GTCAGACGTT TAAAAAGAGG TCGGACTTTC AGAGCAATGA CGATTATGCT 
GTTTACGTGA GAGAGAACAT TCAGGTGGGC ATGATGGTAC GGTGTTGCAG GACATATGAG 
GAAGTGTATG AAGGAGATGT GGGGAAAGTG ATCAAGCTTG ACAGAGACGG CCTGCATGAC 
CTGAATGTTC AGTGTGACTG GCAGCAGAAA GGAGGAACTT ACTGGGTCCG TTACATCCAC 
ACTGAGCTGC TGGGGTTTCC CCCTCAGAGT TCTCCATCTC ATATAAAGAT TGGTGATAAA 
GTGCGAGTGA AGCCCTCGGT CACAACACCA AAATACAAGT GGGGGTCAGT CACTCATAGG 
AGTGTGGGTG TGGTCAAAGC CTTCAGTGCC AATGGGAAGG ATGTCATTGT GGACTTTCCT 
CAGCAGTCGC ACTGGACTGG ACTGCTGTCA GAGATGGAGC TTGTGCCCAG CGTACATCCT 
GGTGTCAGGT GTGACGGATG TCAGATGTTC CCTATAAATG GCCCAAGGTT TAAGTGTCGG 
AGCTGTGATG ACTTTGACTT TTGTGAGAGC TGCTTCAAGA CCCGTAAACA CAATCCTAGG 
CATTCATTTG GGAGGATAAA CGAACCAGGT CAGTCACCCA CCTTTAGTGG GCGCTCAGGA 
AAGCAGCTGA AAAGACATCA CAGCAGTCAG CGTGGCATGC TGATCGACGA GTGGTCAAGA 
GTGGTGAAGA ACCTCAGCGT ATCGTCTTCT GTTAACCAGG CGTCTCGACT CATCGACTGT 
GGAGAGCAAT GCTGGCAGTC CTCAGGCTCT CAGGGCAAGC ACTGGATCAG ATTGGAGCTT 
TTCCCAGACA TGTTAGTCCA CAAACTGAAA ATGATTGTAG ATCCAGCTGA CAGCAGCTAC 
ATGCCTTCAT TGGTGGTGGT ATCAGGTGGA ACCTCACTCA ACAATTTGAT CGAGTTGAAG 
ACCATCAACA TCAATCCCAC TGACACTGTC GTCTCTCTAC TCAATGACTG CACAGAGTAT 
CACAGGTACA TTGAGGTTGC TATAAAGCAG TGTCGAAGTT CAGGTATTGA CTGTAAGATT 
CATGGCTTGA GTGCTGTGGG ACGTGTCCGT GCCGAGGATG AAGATCTTGC AACTGTACCT 
TTCCTGGCAT CTGACAACGA GGAAGAGGAA GATGAGAAGA CCGCGACTGG AAGTATGTCT 
CGAAAGAGAT TGTCAGGCCT CGAGTCTGCA GCCACTATCA GAACCAAAGT CTTTGTCTGG 
GGTCTTAATG ATAAGGACCA ACTTGGAGGA CTTAAGGGGT CCAAGATCAA AGTCCCTTCA 
TTCTCAGAGA CTCTGTCTGC TCTGAATGTA GTGCAGGTGG CTGGTGGCTC TAAAAGTCTT 
TTTGCTGTGA CTGCTGAAGG TAAAGTATAT GCCTGTGGAG AGGCCACCAA TGGAAGACTT 
GGTTTAGGCC TCTCCAGTGG AACCATCCCA ATTCCCAGAC AGATATCAGC TCTCAGCAAC 
TATGTGGTCA AGAAGGTTGC TGTGCATTCA GGTGGACGGC ATGCCATGGC ACTTACCGTG 
GATGGGAAAG TATTCTCTTG GGGTGAAGGA GACGATGGCA AGCTTGGACA CTTCAGCCGA 
ATGAACTGTG ATAAGCCAAG GCTGATCGAG GCATTGAAAA CCAAACGCAT TCGAGATATT 
GCGTGTGGCA GCTCTCACAG TGCCGCCATC ACCTCCAGTG GAGAGCTGTA CAGCTGGGGT 
CTGGGGGAAT ATGGCCGTCT GGGCCATGGG GACAACACCA CCCAACTCAG ACCCAAACTG 
GTCAAGGTGC TTTTAGGCCA TCGCGTTGTC CAGGTAGCTT GTGGAAGTAG AGATGCCCAA 
ACTCTTGCAC TGACAGATGA AGGAATGGTG TTCTCATGGG GAGATGGAGA CTTTGGGAAA 
CTGGGCCGTG GTGGCAGCGA GGGATGTAAC ATTCCTCAAA ACATTGAGCG TCTGAATGGA 
CAGGGGGTCT GTCAAATTGA ATGTGGAGCT CAATTCTCCC TCGCCCTCAC CAAATCTGGA 
GTTGTCTGGA CCTGGGGTAA AGGGGATTAC TTCAGACTGG GTCACGGCAC AGATGTTCAT 
GTCCGCAAAC CCCAGATGGT TGAAGGACTT AGGGGGAAGA AGATTGTTCA TGTGGCCGTT 
GGTGCTCTGC ACTGCCTGGC TGTCACCGAG ACTGGACAGG TGTTCGCGTG GGGGGATAAT 
GACCATGGGC AGCAGGGGAA TGGCACAACC ACAGTCAACA GGAAGCCCAC ATTGGTGCAG 
GGTCTAGAGG GTCAGAAAAT TACTCGTGTG GCCTGCGGTT CTTCTCATAG TGTTGCCTGG 
ACCACAGTAG ATGTCACTAC ACCCTCCGTC CATGAGCCTG TGCTCTTCCA GACTGCTAGA 
GACTCTCTGG GAGCCTCTTA CCTTGGTGTG CCATCAGATA GTGACCCGTC TTTACTGAGC 
AATAAGCTAA ATGGCGTAAA CAATGTGAAG CCCAACAGGC CATCTCTGGC AAAGATCCTT 
CTCTCTCTGG ATGGTAATCT GGCCAAACAG CAGGCCCTAT CCCATGTGCT GTCTTCCCTG 
CAGATCATGT ATGCCAGAGA TGCAGTGGTG GGTGCATTAA TGCCATCCTG TATGATGCCT 
CTGGAGTGTC CCTCCAGCTC TCCGGTGCCC CCATCAGACT GTTCATCAGC TTCCAGCCCC 
TGTGAGGCTG AGGGTCCTGC CCTGCCCTCA GAGAGTGAGG AGAGGGTCAG TCCGCACCCC 
TGGATGGACA GCAAACGAGG AGAGGTAACC ACGTCTGAGG ATGCCACTAC CCCATCTTCA 
GCAGTGACCC CTTCTACTGC CTCTGCCTCA TCTCGACCCT TCATACCAGT CACAGATGAT 
CCTGGTGCTG CCAGCATCAT TGCTGAGACC ATGACCAAAA CCAAAGAGGA CTCTGAGAAC 
CAGAGTAAAG CCGTGGGTCC AGAGCCACAG TATCTGGATG AGTTCACCAG TCTCCTGATC 
CCTGATGACA CGCGTGTGAT GGTGGACCTG TTAAAACTAG CTGTATCTCT GCGCTCTGGT 
GATAAAGGAA AGGAAGTGCT GTCTGCTGTT CTGTCTGGCA TGGGTACAGC CTGCCCACAG 
GTGGCGGATA TGCTTCTGGA GCTGTGTGTG ACTGAGTTGG AGGACGTGGC GACTGACTGT 
CAGAGTGGAC GGCTTTCCTC TCAACCTGTG GTGGTGGAAA GCAGCCATCC ATATATTGAC 
GATACATCCA CCAGCGGAAC TGTCAAAATA CCAGGTGCTG AGGGTTTGCG GGTTGAGTTT 
GATCGCCAGT GCTCAACAGA AAGGAGACAT GACCCTCTTA CCATCATGGA TGGAGCCAAT 
AGAATTGTGT CTGTTCGATC AGGTCGTGAG TGGTCTGATT GGTCCAGTGA ATTGAGAATT 
CCAGGAGACG AGCTCAAATG GAAGTTCACC AGTGACGGCT CTGTGAACGG CTGGGGCTGG 
CGCTTCACAG TCTATCCCAT CATGCCTGCG GCTGGTCCTA AAGATCTTCT GTCAGACCGC 
TGCATTTTGT CTTGCCCTTC AATGGATCTA GTAACATGTC TGCTAGACTT TCGTCTGAAC 
TTTGCCTCAA ACCGCAGCAT TGTGCCCAGA TTAGCAGCCT CACTTGCTGC TTGTGCGCAG 
CTCAGCGCCT TGGCGGCTGG CCATCGTATG TGGGCTCTGC AGAGGTTACG CAAGCTCTTA 
ACTACTGAAT ACGGCCAATC CATTAACATC AACCGCCTCT TAGGAGACAC AGAAGGAGAG 
GCTCGCTCTA TGAGTTTTAC AGGAAGTGCA TTAGCTGCTC TGGTGAAAGG ACTTCCTGAG 
GCATTACAGA GACAGTATGA GTACGAGGAG CCCATAGTTA GGGGTGGCAA ACAGTTACTC 
CACAGCCCGT TCTTCAAGGT GTTGGTGGCG CTGGCGTGTG ATCTGGAGTT GGACACTCTT 
CCATGTTGTG CTGAGACACA CAAGTGGGCG TGGTTTCGAC GCTATTGCAC CGCCTCCAGA 
GTGTCAGTGG CTCTGGACAA GAGGAGCCCT TTGCCCCGCC CTTTTCTGGA TGAGGTAGTG 
GCAAAGAAAA TCCGTGAGCT GATGGCAGAT CATGAGAACA TGAACACGCT TCATGAGAGC 
CATGACTTGT TTAAGAGGGA GCAGGATGAG CAGTTGGTTC AGTGGATGAA CAGACGACCA 
GATGATTGGA CACTCTCTGC GGGTGGCAGT GGTACAATAT ACGGCTGGGG GCACAATCAC 
CGGGGTCAGT TAGGTGGCAT TGAAGGAGCC AAAGTAAAGG TTCCCACACC AACAGAAGCT 
CTAGCCACAC TGCGGCCAGT TCAGCTTATT GGAGGAGAGC AAACACTGTT TGCCGTCACT 
GCTGATGGGA AGCTGTATGC CACAGGATAT GGTGCCGGTG GTCGACTGGG AATAGGAGGG 
ACAGAGTCCG TCTCCACTCC GACCCTGCTG GAGTCCATCC AGCATGTTTT CATCAGAAAG 
GTGGCTGTGA ACTCTGGAGG GAAACACTGT TTGGCTCTTT CCTCTGAAGG AGAGCTGTAC 
TCCTGGGGGG AGGCCGAAGA TGGAAAACTG GGCCATGGAA ACAGAAGCCC ATGTGATCGT 
CCACGCGTGA TTGAGTCTCT CAGAGGGGTG GAAGTGGTGG ACATTGCTGC CGGTGGGGCT 
CACAGTGCCT GCATTACTGC CAGTGGAGAG CTCTACACAT GGGGCAAAGG TCGCTATGGG 
CGCCTGGGTC ATGGAGACAG CGAAGACCAA CTTAAACCAA AACTGGTGGA GGCTCTTCAG 
GGTCACCGGG TGATAGATGT GGCATGTGGC AGTGGAGATG CACAGACCCT CTGTCTGACT 
GATGATGACA TGGTATGGTC GTGGGGCGAT GGAGACTATG GCAAATTGGG CCGAGGAGGT 
AGTGATGGAT GTAAAGTGCC GATGAAGATC GATTCATTAA CCGGATTGGG TGTTGTGAAA 
GTGGAATGTG GCTCTCAGTT TTCTGTGGCC CTTACCAAGT CTGGAGCAGT TTACACATGG 
GGGAAGGGTG ACTATCACAG ACTGGGACAC GGCTCAGATG ATCATGTTCG CAGACCCCGG 
CAGGTCCAGG GTTTACAAGG CAAAAAGGTC ATCGCAATAG CAACTGGTTC CCTTCACTGC 
GTTTGCTGCA CGGAGGACGG GGAAGTGTAC ACATGGGGTG ACAATGATGA GGGTCAGCTT 
GGGGACGGTA CGACCAATGC TATTCAGAGA CCCAGGCTGG TGGCAGCGCT GCAGGGCAAG 
AAGATTAATC GTGTGGCCTG TGGTTCAGCA CATACACTTG CCTGGTCCAC TAGTAAACCA 
ACCAATGCTG GAAAACTCCC TGCTCAGGTT CCAATGGAGT ACAACCACCT GCAAGAAATC 
GCCATCATCG GCCTGAGGAA TCGGTTACTG CTACTGCACC ACATCTCTGA GCTCTTCTGC 
CCCTGCATCC CAATGTTTGA CTTAGAAGGA CGCCTGGGTG AAACTGGACA TGGTGTGTCT 
GTTGGCTTTG ACACGCTCCG TGGAATCCTC ATATCACAGG GCAAGGAGGC AGCATTCCGC 
AAAGTGGTGC AGGCTACCAT GGTCAGAGAC AGGCAGCATG GGCCAGTGGT TGAGCTGAAC 
AGGATACAGG TCAAGCGTTC ACGTAGTAAA GGAGGTCTGG CAGGGCCGGA TGGCACCAAG 
TCAGTATTTG GGCAGATGTG TGCTAAGATG AGTTCTTTCA GTCCAGACAG TCTGCTGCTG 
CCACACCGCG TCTGGAAGGT CAAGTTTGTG GGTGAGTCGG TGGATGACTG TGGTGGCGGA 
TACAGTGAAT CTATCGCAGA AATGTGCGAG GAGCTTCAGA ATGGCCTCAC TCCTCTGCTC 
ATCGTCACTC CTAATGGCAG AGACGAGTCA GGAGCGAACA GAGATTGCTT TCTGCTCAAC 
CCAGCTGCCA AATCCCCTCT GCACATCAGC ATGTTCCGCT TCCTAGGGGT GCTCCTGGGC 
ATAGCGATCC GAACAGGAAG TCCTCTTAGT CTGTATCTGG CCGAACCTGT GTGGAAACAG 
CTGGCAGGCA TGAACCTGAC CATTGCTGAT CTCAGTGAGG TGGACAAGGA CTTCATCCCC 
GGTCTGATGT ATATTAGAGA TAACGAGGCG TCTGCGGAGG AGTTTGAGGC GATGCCTCTT 
CCCTTCACTG TGCCCAGTGC CTGCGGACAA GAAGTCCAGC TCAGCTCCAA ACATTCCCAC 
ATCAGCCTTG AAAACCGCAG TGAATATGTG CGGCTAGCAA TAAATTACAG GCTGCATGAG 
TTTGATGAGC AGGTGTCTGC TGTAAGAGAA GGTATGGCGC GTGTGGTTCC GGTACCGCTC 
CTCTCTCTAT TTACCGGCTA TGAGCTGGAG ACCATGGTGT GCGGAAGTCC GGACATCCCT 
CTGCATCTGC TCAAATCAGT GGCCACATAT AAAGGAGTAG AGCCCACTGC ACCGCTCATC 
CAGTGGTTCT GGGAGGTGAT GGAGTCTTTC TCTAACACAG AACGCTCGCT CTTCCTCAGG 
TTCGTCTGGG GCCGCACGCG TCTTCCCAGG ACCATCGCAG ACTTCCGTGG ACGAGACTTT 
GTAGTGCAGG TGCTGGATAA ATATAACCCT CCCGACCACT TCCTCCCCGA GTCCTACACC 
TGCTTCTTCC TGCTCAAGCT GCCTCGCTAC TCCTGCAAGC TAGTCTTGGA GGAGAAACTC 
AAATACGCCA TTCACTTCTG CAAGTCCATC GACACTGATG ACTACGCACG GATCGCTCTG 
AGCGGAGAAC CGGCCGCAGA CGACAGCAGT GATGACTCGG ACAATGAAGA CGCAGACTCA 
TTCGCTTCTG ACTCCACACA GGATTACCTG ACAGGCCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212137.1
CDS	144..14645
Translation	MPSPSFSLNAQLRFHDKWLKIDLQRAFSHEGLSEMWNEMVKDEEIVFDREESAHPEDCADCTQKKDDTNDKEKKEEEEAAATAASSFHHSIIEMWDWGRQPETSELRDCLMALVDDQQKLSSQTAKSTLSALRLSQRLVILERYLISLTHSMMEEKYKVRWKSPPSPPLSAVDNKSARPVSKGVEGLARVGSRAALSFAFAFLRRAWRSGDDADLCSELLQESLDALRALPEATLFDEGTVSPVWLEVVERATKFLSDIHGSASGKAPSNIPLQDQHLALAILLELAVQRGTLSQLLSAVLLLLRLWDSGTREMDNERSTQGTSAPLLPLLQRFQNIHSSKEEPVPEEEIEILSAPLSPNESFLRYLTLPQDNDLAIDLRQTAVVIMAHLDRLAAPCSPPQCSSPTSNKGTLQEVIGWGLLGWKPYANVNGPIQCKALSNLGVTQIVCSEKGFLILTITGAVYTQNYKSTTLAPMLVHALTSRKIMKLAAHPDGQHYLALSVNGEVFSWGCGDGGRLGHGDTTYLEEPTMISVFSGRPVGKQVVHIACGSTYSAAITADGELHTWGRGNYGRLGHGSSEDQTTPMLVTALKGLKVIDVACGSGDAQTLAVTENGQVWSWGDGDYGKLGRGGSDGCKTPKLVEKLQDLDIVKVCCGSQFSVALTKDGQVYTWGKGDNQRLGHGTDEHVRYPKLLDSLQGKRVVDIAVGSTHCLALTEEGEVHSWGSNDKLQHFDTLFSNKKQPKALPGLNSKHIVGISCGPGQSFSWSSCSEWSVGLRVPFVVDVCTMTFDQLDLLLRQVSEGMDGSSDWPPPQEKECMVVAALNLLRLQLHAAISNQVDPEELGLGLGSVLLNNLKQTVVTLASNGGVLNTVQAAAQTVLQSGWSVLLPTAEERARALSSLLPNAASANEMSVSPGRRFMIDLLVSSLMADGGLESALNAAITAEIQDIEAKKEAQKEKEIDEQEASASSLHRCRTTLDKDLINTGIYESANKQSLPLVQLVQQLLRNIASQTIARLKDVARRISNCQDTEQVSKERSASLDLLLRFQRLLVCKLYVGVNGTVGVGGYTLSHSEILGVGSLLKKYMALLCTHIGDILPVATSIALNSHRHFAEVARVLEGDLTGVLLPELIVSIVLLLTHDAGLMQETGSIPLLAALLEHLDRFNYLAPGTERDDNEDLAWPGLMGFFFNGPSSKNNEEVSLIRKADLENHNKDGGFWTVIEGKVYDIKDFQAQTLTGNSILAQFAGEDPVVALEAALQFEDTRESMQAFCVGQYMEPNQEMVTTPDLSSLSSPLIDAERNLGLLLGLHASYLAMSTPLSRIELECAKWLQSSIFFGGLQTSQIHYNYNEEKDEDHCSSPGTTTPDKAKLYCRRITLSDHAQPFLQAIADNTTQDHTVKDFLCQIERCCKQYHLITPITFPPEHPVEEVGRLLLCCLLKHQDLGHVALSLVDQCALGMDQGKQRSLPKSVIDVCRMVYQAKCSLIKTHQEQGRSYKEVCAPVIERLRFLFNELRPAVSNDLSIVSKLKLLSSQPRWKRITQKLIRDRRKKRVPKKSESADLEECKMEREGTNEEPPAPLSPAPVDKKQTTVKSAKQDKWQPLLNTIGSVQKYRWLRHSVQGSFSQSSLMSTIVEFSLKEEPLDVEKIRKCLLKQLERAEVRLEGIDTMLKLASKNFLLPSVQYAMLCGWQRVIPEGTNIGEPLADCLKDVDLIPPFNRMLLEVTFGKLYAWSIQNVRNILLEASVKFKELGVQPVPLQTITNENPAGPSLGTIPQARFLLAMLHMLTLKHGANSLSLLLNSGILALTQSVLRLIGPSCDSSEEELTSVAHGGSATVLEESRKETAPTPLPASGPELAAMMKISTRVMRGPDWKWGDQDGPSPGLGRVIGELGEDGWIRVQWDTGSTNSYRMGKEGKYDLKLAEPPPATQPATEDSDTEDDTEGEVMEKCAHPTAIMLTSTVNLLKTLSLSSGIHAEVMQVDAVSTLCGLLRILVESGANDKTGCSSHKLVSREQHRSWCTLGFIRSIALMPQMCSTLSSSAWINLLIRIVEGHQSFTAITLQRQILALRLLQAVLPSWDKTERSQDMKFLVEKLFGFLGSLLSTCSSDLPLLREGSMRRRKSRPQASLTATHSSTLAEEIVTVLRTLHSLSQWNALINEYINSQLSGIGDVMAGRHTEVALLEDYLPNAEAAAVGSLMAVLAVIGGIDGRLRLGGRVVHEEYGEGTVTRITPKGRITVQFHEMRNCKVCLLSQLKPLHMIPFSVQNLPFAEPMLMVWAQLVNLAGSKLEKQRMKKSLSRGLTADQVDVHLLRCQQLRLYILKAARALLSHQDKLRQILSQAAVVDVSPNPTEDQCASSPDVGDLSPEGPQPPLILLQQLLSASTQPSPIKAIFHRQELEAAALAVCQYLAVESTHPSSPLFEDSSSSEATTPVTVQHMRPPKQKKHKTSPVPPAPIVLQLMEMGFQRKNIEFALKSLSGTTGASGVPGVEALVGWLLDHPDVLITDLSDAETVSDEYSDEEVLEELEEPEPAFPVPPGVVVTESQTFKKRSDFQSNDDYAVYVRENIQVGMMVRCCRTYEEVYEGDVGKVIKLDRDGLHDLNVQCDWQQKGGTYWVRYIHTELLGFPPQSSPSHIKIGDKVRVKPSVTTPKYKWGSVTHRSVGVVKAFSANGKDVIVDFPQQSHWTGLLSEMELVPSVHPGVRCDGCQMFPINGPRFKCRSCDDFDFCESCFKTRKHNPRHSFGRINEPGQSPTFSGRSGKQLKRHHSSQRGMLIDEWSRVVKNLSVSSSVNQASRLIDCGEQCWQSSGSQGKHWIRLELFPDMLVHKLKMIVDPADSSYMPSLVVVSGGTSLNNLIELKTININPTDTVVSLLNDCTEYHRYIEVAIKQCRSSGIDCKIHGLSAVGRVRAEDEDLATVPFLASDNEEEEDEKTATGSMSRKRLSGLESAATIRTKVFVWGLNDKDQLGGLKGSKIKVPSFSETLSALNVVQVAGGSKSLFAVTAEGKVYACGEATNGRLGLGLSSGTIPIPRQISALSNYVVKKVAVHSGGRHAMALTVDGKVFSWGEGDDGKLGHFSRMNCDKPRLIEALKTKRIRDIACGSSHSAAITSSGELYSWGLGEYGRLGHGDNTTQLRPKLVKVLLGHRVVQVACGSRDAQTLALTDEGMVFSWGDGDFGKLGRGGSEGCNIPQNIERLNGQGVCQIECGAQFSLALTKSGVVWTWGKGDYFRLGHGTDVHVRKPQMVEGLRGKKIVHVAVGALHCLAVTETGQVFAWGDNDHGQQGNGTTTVNRKPTLVQGLEGQKITRVACGSSHSVAWTTVDVTTPSVHEPVLFQTARDSLGASYLGVPSDSDPSLLSNKLNGVNNVKPNRPSLAKILLSLDGNLAKQQALSHVLSSLQIMYARDAVVGALMPSCMMPLECPSSSPVPPSDCSSASSPCEAEGPALPSESEERVSPHPWMDSKRGEVTTSEDATTPSSAVTPSTASASSRPFIPVTDDPGAASIIAETMTKTKEDSENQSKAVGPEPQYLDEFTSLLIPDDTRVMVDLLKLAVSLRSGDKGKEVLSAVLSGMGTACPQVADMLLELCVTELEDVATDCQSGRLSSQPVVVESSHPYIDDTSTSGTVKIPGAEGLRVEFDRQCSTERRHDPLTIMDGANRIVSVRSGREWSDWSSELRIPGDELKWKFTSDGSVNGWGWRFTVYPIMPAAGPKDLLSDRCILSCPSMDLVTCLLDFRLNFASNRSIVPRLAASLAACAQLSALAAGHRMWALQRLRKLLTTEYGQSININRLLGDTEGEARSMSFTGSALAALVKGLPEALQRQYEYEEPIVRGGKQLLHSPFFKVLVALACDLELDTLPCCAETHKWAWFRRYCTASRVSVALDKRSPLPRPFLDEVVAKKIRELMADHENMNTLHESHDLFKREQDEQLVQWMNRRPDDWTLSAGGSGTIYGWGHNHRGQLGGIEGAKVKVPTPTEALATLRPVQLIGGEQTLFAVTADGKLYATGYGAGGRLGIGGTESVSTPTLLESIQHVFIRKVAVNSGGKHCLALSSEGELYSWGEAEDGKLGHGNRSPCDRPRVIESLRGVEVVDIAAGGAHSACITASGELYTWGKGRYGRLGHGDSEDQLKPKLVEALQGHRVIDVACGSGDAQTLCLTDDDMVWSWGDGDYGKLGRGGSDGCKVPMKIDSLTGLGVVKVECGSQFSVALTKSGAVYTWGKGDYHRLGHGSDDHVRRPRQVQGLQGKKVIAIATGSLHCVCCTEDGEVYTWGDNDEGQLGDGTTNAIQRPRLVAALQGKKINRVACGSAHTLAWSTSKPTNAGKLPAQVPMEYNHLQEIAIIGLRNRLLLLHHISELFCPCIPMFDLEGRLGETGHGVSVGFDTLRGILISQGKEAAFRKVVQATMVRDRQHGPVVELNRIQVKRSRSKGGLAGPDGTKSVFGQMCAKMSSFSPDSLLLPHRVWKVKFVGESVDDCGGGYSESIAEMCEELQNGLTPLLIVTPNGRDESGANRDCFLLNPAAKSPLHISMFRFLGVLLGIAIRTGSPLSLYLAEPVWKQLAGMNLTIADLSEVDKDFIPGLMYIRDNEASAEEFEAMPLPFTVPSACGQEVQLSSKHSHISLENRSEYVRLAINYRLHEFDEQVSAVREGMARVVPVPLLSLFTGYELETMVCGSPDIPLHLLKSVATYKGVEPTAPLIQWFWEVMESFSNTERSLFLRFVWGRTRLPRTIADFRGRDFVVQVLDKYNPPDHFLPESYTCFFLLKLPRYSCKLVLEEKLKYAIHFCKSIDTDDYARIALSGEPAADDSSDDSDNEDADSFASDSTQDYLTGH

Target ORF information:

RefSeq Version	XM_017356648.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio HECT and RLD domain containing E3 ubiquitin protein ligase 2 (herc2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356648.1

ORF Insert Sequence:

ATGCCTTCTC CATCATTTTC CCTGAATGCC CAGCTGCGCT TCCATGATAA ATGGCTGAAG 
ATCGACTTGC AGCGCGCCTT CTCTCATGAA GGACTGAGCG AGATGTGGAA CGAGATGGTG 
AAGGATGAAG AGATCGTCTT TGACAGAGAA GAGTCTGCCC ATCCAGAGGA CTGTGCTGAT 
TGCACCCAAA AGAAAGATGA CACCAATGAT AAAGAAAAGA AGGAAGAGGA GGAGGCAGCA 
GCAACGGCGG CTTCATCTTT CCATCACTCC ATCATTGAAA TGTGGGACTG GGGGAGGCAG 
CCAGAAACCA GCGAGCTGCG GGACTGTCTG ATGGCTTTGG TAGACGACCA ACAGAAGCTG 
TCTTCTCAGA CGGCTAAGAG CACACTCTCA GCCCTGCGTC TAAGCCAGAG ACTGGTGATC 
CTGGAGCGCT ACCTCATCTC GCTCACTCAC AGCATGATGG AGGAGAAGTA CAAAGTACGG 
TGGAAGAGCC CTCCCAGTCC TCCACTCTCT GCTGTCGACA ACAAAAGCGC TCGGCCAGTT 
AGTAAAGGGG TGGAAGGTTT GGCCAGGGTG GGCTCAAGGG CAGCTCTTTC CTTTGCCTTT 
GCATTTCTCC GCCGAGCCTG GAGATCAGGA GATGATGCAG ACCTGTGCAG TGAGTTACTG 
CAGGAGTCTC TGGATGCTCT GCGGGCTCTT CCAGAAGCTA CTCTGTTTGA TGAGGGCACT 
GTATCTCCTG TTTGGCTGGA GGTGGTTGAG AGGGCTACTA AATTTCTAAG TGACATCCAT 
GGAAGTGCCA GTGGAAAAGC ACCCAGTAAC ATTCCCTTGC AGGATCAGCA CTTGGCCCTT 
GCCATCCTGC TGGAGCTCGC AGTGCAACGA GGAACTCTCA GTCAGCTGCT GTCTGCAGTT 
CTGCTGCTTC TGAGGCTCTG GGACAGTGGA ACTAGAGAGA TGGATAATGA GCGCTCCACC 
CAAGGCACCA GCGCCCCGTT ACTGCCCCTC CTGCAGCGCT TCCAGAACAT CCACAGCAGT 
AAAGAGGAGC CTGTTCCTGA AGAGGAGATT GAGATCCTTT CGGCCCCTCT GAGCCCGAAT 
GAGAGTTTTC TGCGCTACCT TACACTGCCT CAAGATAATG ACCTGGCCAT TGATTTGCGG 
CAGACAGCTG TAGTCATCAT GGCACATTTG GACCGCCTGG CAGCCCCATG CAGCCCCCCG 
CAGTGCAGCT CACCCACTTC TAACAAGGGG ACACTGCAGG AGGTGATTGG TTGGGGTCTG 
CTTGGATGGA AGCCTTATGC TAATGTGAAT GGACCAATTC AGTGCAAGGC ACTATCAAAT 
CTGGGTGTCA CGCAGATTGT CTGCTCAGAG AAGGGTTTCC TCATCCTGAC CATCACTGGT 
GCTGTCTACA CACAAAACTA CAAAAGTACT ACACTAGCAC CTATGCTAGT CCATGCACTG 
ACGTCCAGGA AAATAATGAA GCTTGCAGCT CATCCTGATG GCCAGCATTA TTTGGCTCTT 
TCAGTCAATG GAGAGGTTTT CTCCTGGGGT TGTGGAGATG GAGGCAGGCT AGGACATGGA 
GACACTACGT ATTTAGAGGA GCCCACTATG ATTTCAGTAT TCTCAGGGAG GCCGGTAGGG 
AAGCAGGTTG TACATATTGC CTGTGGCAGC ACATACAGCG CAGCCATCAC AGCAGATGGA 
GAGCTTCACA CATGGGGCCG AGGAAACTAC GGCAGACTGG GCCATGGCTC CAGTGAGGAC 
CAGACCACTC CCATGCTAGT AACTGCACTG AAGGGGCTGA AGGTGATCGA TGTGGCCTGT 
GGAAGCGGAG ATGCTCAGAC GCTTGCTGTG ACTGAGAATG GTCAGGTATG GTCATGGGGT 
GATGGAGATT ATGGGAAACT GGGCCGAGGG GGCAGTGATG GATGCAAAAC ACCAAAACTA 
GTGGAGAAGC TCCAGGATCT GGATATTGTG AAGGTGTGCT GTGGGAGCCA GTTCTCCGTA 
GCGCTCACTA AAGATGGACA AGTTTACACC TGGGGCAAAG GAGACAATCA ACGACTTGGA 
CATGGCACTG ATGAACATGT CCGCTATCCA AAACTACTTG ACAGTCTGCA AGGCAAGAGG 
GTGGTGGACA TAGCGGTTGG CTCCACACAC TGCCTGGCCC TTACAGAAGA AGGAGAGGTG 
CACAGCTGGG GCAGTAATGA CAAACTACAG CACTTTGACA CACTTTTCTC CAATAAGAAG 
CAGCCCAAAG CCTTGCCAGG CCTCAACTCC AAACACATAG TAGGCATCTC CTGCGGTCCT 
GGCCAGAGTT TTTCATGGTC ATCCTGCTCT GAGTGGTCTG TGGGTCTCCG GGTACCCTTC 
GTGGTAGACG TGTGCACTAT GACCTTTGAC CAGCTGGACC TGCTTCTGCG CCAGGTTAGT 
GAGGGCATGG ACGGCAGCTC TGATTGGCCA CCACCACAGG AGAAAGAATG CATGGTCGTG 
GCAGCTCTCA ACCTGCTCAG ACTACAGCTT CATGCAGCCA TCAGTAATCA GGTGGATCCC 
GAGGAGCTGG GTCTGGGTCT GGGCAGTGTG CTGCTTAATA ACCTCAAGCA GACGGTGGTG 
ACTCTGGCCA GCAACGGCGG GGTGCTAAAC ACTGTGCAGG CTGCCGCCCA GACCGTGCTC 
CAGAGTGGCT GGTCAGTGCT ACTCCCCACT GCAGAGGAAC GAGCAAGAGC CCTGTCCTCG 
CTGCTGCCCA ATGCAGCATC TGCTAATGAG ATGAGTGTAA GTCCTGGCCG TCGCTTCATG 
ATCGACCTCT TGGTTAGTAG TCTGATGGCA GATGGAGGGT TGGAGTCTGC TTTGAATGCT 
GCCATCACGG CCGAGATCCA GGATATTGAG GCAAAGAAAG AGGCTCAGAA AGAGAAGGAA 
ATCGATGAGC AGGAAGCCAG CGCCTCTTCT TTGCATCGCT GTCGCACCAC ACTGGACAAA 
GACCTGATCA ACACCGGCAT CTATGAGTCT GCCAACAAAC AGAGCCTCCC ATTGGTCCAG 
CTTGTCCAAC AGCTGCTCAG GAACATTGCT TCACAGACTA TAGCCCGTCT TAAGGATGTG 
GCCCGACGCA TCTCAAATTG TCAAGACACC GAGCAGGTCA GCAAGGAGCG CTCAGCCTCT 
CTGGACCTGC TGCTGCGCTT TCAGAGGCTT TTGGTCTGCA AATTGTATGT CGGAGTCAAT 
GGCACTGTGG GTGTGGGAGG ATACACTTTG AGTCACTCAG AAATTCTGGG AGTTGGCTCT 
CTCCTGAAGA AGTATATGGC TTTGCTTTGC ACACACATCG GTGATATCCT TCCTGTGGCC 
ACCAGCATTG CCCTCAACAG CCATCGTCAC TTTGCAGAGG TGGCTCGTGT CCTTGAGGGA 
GATCTTACAG GTGTTCTGCT ACCAGAGCTC ATTGTGTCCA TTGTGCTGCT GCTGACCCAT 
GATGCTGGAC TAATGCAAGA GACTGGATCC ATTCCTCTGC TCGCTGCTCT TCTGGAGCAC 
TTGGACCGTT TTAACTATCT TGCACCTGGT ACAGAAAGAG ATGACAATGA GGATCTTGCC 
TGGCCTGGAC TCATGGGTTT CTTTTTCAAT GGACCGAGCT CAAAGAACAA CGAGGAGGTG 
TCACTTATCC GCAAAGCAGA CCTGGAGAAT CACAACAAAG ATGGCGGCTT TTGGACTGTA 
ATTGAAGGGA AGGTCTATGA CATAAAGGAC TTTCAAGCTC AGACTCTAAC TGGCAACAGC 
ATCCTTGCCC AGTTTGCAGG AGAAGACCCT GTGGTGGCAT TAGAGGCTGC CCTCCAATTT 
GAAGACACCA GAGAGTCCAT GCAAGCTTTC TGTGTGGGAC AGTATATGGA GCCCAACCAA 
GAAATGGTGA CAACGCCTGA CCTGAGCAGC CTGTCCTCTC CTCTTATAGA TGCTGAGAGG 
AACCTTGGTC TGCTTCTGGG GCTACATGCC TCATATCTGG CTATGAGCAC CCCTCTCTCC 
CGCATAGAGT TAGAATGTGC CAAATGGCTC CAGTCATCTA TTTTCTTCGG TGGACTTCAG 
ACCAGTCAGA TCCACTATAA TTATAACGAG GAGAAGGATG AGGACCACTG CAGCTCTCCA 
GGGACCACCA CACCAGACAA AGCCAAACTG TACTGCCGCA GAATCACCCT GAGTGATCAC 
GCCCAGCCCT TCCTGCAGGC CATCGCTGAC AACACCACCC AGGATCACAC TGTGAAGGAC 
TTTTTATGCC AAATCGAGCG GTGCTGTAAA CAGTACCATC TCATCACTCC CATCACTTTT 
CCACCTGAAC ATCCTGTGGA GGAAGTGGGT CGCCTGCTGC TCTGCTGCCT TCTGAAGCAT 
CAGGATCTTG GTCATGTCGC CCTCTCCCTA GTTGATCAAT GTGCCTTGGG TATGGACCAG 
GGGAAACAGA GGTCCCTCCC GAAATCTGTG ATTGATGTGT GTCGAATGGT GTATCAGGCC 
AAGTGTTCGT TGATAAAAAC ACACCAGGAA CAGGGTCGTT CTTATAAAGA GGTGTGCGCT 
CCTGTTATTG AGAGGTTGCG TTTCCTGTTT AATGAGCTAC GACCTGCCGT CAGTAATGAC 
TTGTCCATTG TGTCCAAACT CAAACTGCTG AGCTCTCAGC CTCGCTGGAA GAGGATCACA 
CAGAAACTGA TCAGAGACCG CCGCAAAAAG AGAGTGCCTA AAAAGTCGGA GTCAGCTGAT 
CTGGAGGAGT GCAAGATGGA GAGGGAAGGG ACCAATGAGG AACCACCTGC TCCTCTAAGC 
CCCGCTCCTG TTGACAAGAA GCAAACAACT GTCAAATCTG CAAAGCAGGA TAAGTGGCAG 
CCTCTGCTAA ACACCATCGG CAGTGTTCAG AAGTACCGCT GGTTAAGGCA CAGCGTTCAG 
GGCTCCTTCT CTCAGTCCAG CCTCATGAGC ACCATCGTTG AGTTTTCTTT GAAAGAGGAG 
CCGCTGGATG TGGAGAAGAT CAGAAAGTGC CTCCTTAAAC AGCTGGAGAG AGCTGAGGTC 
CGCCTGGAGG GTATTGACAC TATGTTGAAG CTGGCATCTA AAAACTTCCT CCTGCCCTCT 
GTTCAGTATG CCATGCTTTG TGGCTGGCAG AGAGTCATAC CTGAAGGGAC CAATATTGGA 
GAGCCATTGG CTGACTGTCT AAAGGATGTA GATCTCATTC CTCCATTCAA CCGCATGCTA 
CTAGAGGTGA CGTTTGGGAA GCTCTATGCC TGGTCCATAC AGAACGTCCG CAACATCTTG 
TTGGAGGCTA GTGTCAAGTT CAAGGAACTG GGTGTGCAAC CAGTGCCTCT CCAGACAATC 
ACTAATGAGA ACCCAGCAGG CCCCAGTCTG GGTACCATCC CTCAGGCACG CTTCCTGCTG 
GCTATGCTCC ATATGCTCAC TCTCAAACAC GGAGCCAACA GCCTCAGCCT CCTGCTGAAC 
TCTGGCATCC TCGCTCTCAC CCAGAGTGTC CTCCGACTCA TTGGTCCTAG CTGTGATAGC 
AGTGAGGAGG AGCTGACCTC TGTAGCTCAT GGAGGCTCTG CCACAGTTCT GGAAGAGTCC 
AGAAAGGAGA CTGCCCCGAC CCCTCTGCCC GCTTCCGGCC CTGAGCTGGC AGCTATGATG 
AAGATCAGCA CAAGGGTGAT GAGAGGTCCT GACTGGAAGT GGGGGGACCA GGATGGCCCA 
TCACCAGGCC TGGGTCGTGT GATTGGGGAG TTGGGAGAAG ATGGTTGGAT AAGAGTCCAA 
TGGGACACCG GCAGCACCAA CTCCTACAGG ATGGGAAAAG AGGGCAAGTA TGACCTTAAA 
CTGGCTGAGC CGCCACCTGC CACCCAACCA GCCACTGAAG ACTCTGACAC GGAGGATGAC 
ACAGAAGGGG AGGTGATGGA AAAGTGTGCC CACCCCACGG CAATCATGCT GACTAGTACA 
GTAAATCTGT TGAAGACACT GTCACTGTCT TCTGGGATTC ATGCAGAGGT GATGCAGGTC 
GATGCGGTCA GTACTCTGTG CGGGCTACTC AGGATACTGG TGGAGAGTGG AGCCAACGAC 
AAGACTGGTT GCTCCTCTCA CAAGCTGGTG TCCCGAGAGC AGCACAGGAG CTGGTGCACG 
CTGGGATTTA TTCGCAGTAT AGCTCTTATG CCTCAGATGT GCAGTACCCT GAGCTCCTCT 
GCCTGGATCA ATCTGCTTAT ACGCATCGTG GAGGGTCATC AGTCCTTCAC TGCAATCACA 
CTACAGAGAC AGATTCTTGC CCTGCGGCTT CTACAGGCAG TATTGCCCTC CTGGGATAAG 
ACTGAACGCT CTCAGGATAT GAAGTTCTTG GTGGAGAAAC TGTTTGGGTT CCTCGGCAGC 
TTGCTCAGCA CTTGTTCTTC AGATCTGCCT CTTCTTAGGG AAGGTTCAAT GCGTAGGAGG 
AAGTCCCGCC CCCAGGCCTC ACTGACAGCC ACTCATAGCA GTACTTTGGC AGAGGAAATA 
GTGACAGTAT TGCGCACGCT TCACTCCCTC AGCCAATGGA ATGCCCTGAT TAATGAATAC 
ATTAACTCTC AGCTCAGTGG AATCGGGGAT GTCATGGCAG GCCGACACAC TGAAGTGGCT 
TTGCTTGAAG ACTATCTCCC CAATGCGGAA GCAGCAGCGG TGGGCAGTCT GATGGCTGTT 
TTGGCAGTCA TCGGGGGGAT TGATGGACGT CTGCGTCTGG GTGGACGTGT GGTCCATGAG 
GAGTATGGAG AAGGCACAGT GACTCGCATC ACACCTAAAG GACGAATCAC TGTTCAGTTT 
CATGAGATGA GGAACTGCAA AGTGTGCCTG CTCAGTCAGC TCAAACCGCT TCACATGATT 
CCATTCAGCG TTCAGAATCT GCCCTTCGCT GAGCCCATGC TAATGGTGTG GGCCCAGCTA 
GTCAACTTGG CTGGGAGCAA GCTGGAGAAA CAACGCATGA AGAAATCTCT TAGTAGAGGA 
CTTACAGCGG ATCAGGTAGA TGTACATTTG TTGCGCTGTC AGCAGCTCAG GCTGTACATC 
TTGAAGGCTG CACGAGCTCT ACTCTCCCAT CAGGACAAAC TCAGACAGAT CCTTTCTCAG 
GCGGCCGTGG TTGATGTCAG CCCCAATCCT ACAGAGGATC AGTGCGCGTC GTCTCCTGAT 
GTAGGAGATT TGTCTCCTGA GGGTCCTCAG CCTCCTCTCA TCCTCCTTCA GCAGTTGCTG 
TCAGCATCCA CTCAACCTTC ACCCATCAAA GCCATATTCC ACCGCCAGGA GTTAGAGGCA 
GCGGCTCTGG CTGTGTGTCA GTACCTGGCG GTTGAATCCA CACACCCGTC ATCGCCTCTG 
TTTGAGGACA GCAGCTCTAG CGAAGCCACT ACACCAGTCA CTGTGCAGCA CATGCGTCCA 
CCCAAACAAA AGAAACACAA GACGTCACCT GTGCCCCCTG CTCCCATCGT CCTGCAGCTG 
ATGGAGATGG GCTTTCAAAG GAAAAACATC GAGTTTGCTC TTAAATCACT GTCTGGAACC 
ACAGGTGCTT CTGGAGTGCC AGGTGTCGAG GCATTGGTGG GCTGGTTGTT GGACCATCCA 
GATGTTCTCA TTACAGATCT GTCGGATGCA GAGACTGTAT CAGATGAATA CTCTGATGAA 
GAGGTGCTGG AAGAGCTTGA GGAGCCTGAG CCTGCCTTTC CTGTGCCTCC TGGGGTTGTG 
GTGACTGAGA GTCAGACGTT TAAAAAGAGG TCGGACTTTC AGAGCAATGA CGATTATGCT 
GTTTACGTGA GAGAGAACAT TCAGGTGGGC ATGATGGTAC GGTGTTGCAG GACATATGAG 
GAAGTGTATG AAGGAGATGT GGGGAAAGTG ATCAAGCTTG ACAGAGACGG CCTGCATGAC 
CTGAATGTTC AGTGTGACTG GCAGCAGAAA GGAGGAACTT ACTGGGTCCG TTACATCCAC 
ACTGAGCTGC TGGGGTTTCC CCCTCAGAGT TCTCCATCTC ATATAAAGAT TGGTGATAAA 
GTGCGAGTGA AGCCCTCGGT CACAACACCA AAATACAAGT GGGGGTCAGT CACTCATAGG 
AGTGTGGGTG TGGTCAAAGC CTTCAGTGCC AATGGGAAGG ATGTCATTGT GGACTTTCCT 
CAGCAGTCGC ACTGGACTGG ACTGCTGTCA GAGATGGAGC TTGTGCCCAG CGTACATCCT 
GGTGTCAGGT GTGACGGATG TCAGATGTTC CCTATAAATG GCCCAAGGTT TAAGTGTCGG 
AGCTGTGATG ACTTTGACTT TTGTGAGAGC TGCTTCAAGA CCCGTAAACA CAATCCTAGG 
CATTCATTTG GGAGGATAAA CGAACCAGGT CAGTCACCCA CCTTTAGTGG GCGCTCAGGA 
AAGCAGCTGA AAAGACATCA CAGCAGTCAG CGTGGCATGC TGATCGACGA GTGGTCAAGA 
GTGGTGAAGA ACCTCAGCGT ATCGTCTTCT GTTAACCAGG CGTCTCGACT CATCGACTGT 
GGAGAGCAAT GCTGGCAGTC CTCAGGCTCT CAGGGCAAGC ACTGGATCAG ATTGGAGCTT 
TTCCCAGACA TGTTAGTCCA CAAACTGAAA ATGATTGTAG ATCCAGCTGA CAGCAGCTAC 
ATGCCTTCAT TGGTGGTGGT ATCAGGTGGA ACCTCACTCA ACAATTTGAT CGAGTTGAAG 
ACCATCAACA TCAATCCCAC TGACACTGTC GTCTCTCTAC TCAATGACTG CACAGAGTAT 
CACAGGTACA TTGAGGTTGC TATAAAGCAG TGTCGAAGTT CAGGTATTGA CTGTAAGATT 
CATGGCTTGA GTGCTGTGGG ACGTGTCCGT GCCGAGGATG AAGATCTTGC AACTGTACCT 
TTCCTGGCAT CTGACAACGA GGAAGAGGAA GATGAGAAGA CCGCGACTGG AAGTATGTCT 
CGAAAGAGAT TGTCAGGCCT CGAGTCTGCA GCCACTATCA GAACCAAAGT CTTTGTCTGG 
GGTCTTAATG ATAAGGACCA ACTTGGAGGA CTTAAGGGGT CCAAGATCAA AGTCCCTTCA 
TTCTCAGAGA CTCTGTCTGC TCTGAATGTA GTGCAGGTGG CTGGTGGCTC TAAAAGTCTT 
TTTGCTGTGA CTGCTGAAGG TAAAGTATAT GCCTGTGGAG AGGCCACCAA TGGAAGACTT 
GGTTTAGGCC TCTCCAGTGG AACCATCCCA ATTCCCAGAC AGATATCAGC TCTCAGCAAC 
TATGTGGTCA AGAAGGTTGC TGTGCATTCA GGTGGACGGC ATGCCATGGC ACTTACCGTG 
GATGGGAAAG TATTCTCTTG GGGTGAAGGA GACGATGGCA AGCTTGGACA CTTCAGCCGA 
ATGAACTGTG ATAAGCCAAG GCTGATCGAG GCATTGAAAA CCAAACGCAT TCGAGATATT 
GCGTGTGGCA GCTCTCACAG TGCCGCCATC ACCTCCAGTG GAGAGCTGTA CAGCTGGGGT 
CTGGGGGAAT ATGGCCGTCT GGGCCATGGG GACAACACCA CCCAACTCAG ACCCAAACTG 
GTCAAGGTGC TTTTAGGCCA TCGCGTTGTC CAGGTAGCTT GTGGAAGTAG AGATGCCCAA 
ACTCTTGCAC TGACAGATGA AGGAATGGTG TTCTCATGGG GAGATGGAGA CTTTGGGAAA 
CTGGGCCGTG GTGGCAGCGA GGGATGTAAC ATTCCTCAAA ACATTGAGCG TCTGAATGGA 
CAGGGGGTCT GTCAAATTGA ATGTGGAGCT CAATTCTCCC TCGCCCTCAC CAAATCTGGA 
GTTGTCTGGA CCTGGGGTAA AGGGGATTAC TTCAGACTGG GTCACGGCAC AGATGTTCAT 
GTCCGCAAAC CCCAGATGGT TGAAGGACTT AGGGGGAAGA AGATTGTTCA TGTGGCCGTT 
GGTGCTCTGC ACTGCCTGGC TGTCACCGAG ACTGGACAGG TGTTCGCGTG GGGGGATAAT 
GACCATGGGC AGCAGGGGAA TGGCACAACC ACAGTCAACA GGAAGCCCAC ATTGGTGCAG 
GGTCTAGAGG GTCAGAAAAT TACTCGTGTG GCCTGCGGTT CTTCTCATAG TGTTGCCTGG 
ACCACAGTAG ATGTCACTAC ACCCTCCGTC CATGAGCCTG TGCTCTTCCA GACTGCTAGA 
GACTCTCTGG GAGCCTCTTA CCTTGGTGTG CCATCAGATA GTGACCCGTC TTTACTGAGC 
AATAAGCTAA ATGGCGTAAA CAATGTGAAG CCCAACAGGC CATCTCTGGC AAAGATCCTT 
CTCTCTCTGG ATGGTAATCT GGCCAAACAG CAGGCCCTAT CCCATGTGCT GTCTTCCCTG 
CAGATCATGT ATGCCAGAGA TGCAGTGGTG GGTGCATTAA TGCCATCCTG TATGATGCCT 
CTGGAGTGTC CCTCCAGCTC TCCGGTGCCC CCATCAGACT GTTCATCAGC TTCCAGCCCC 
TGTGAGGCTG AGGGTCCTGC CCTGCCCTCA GAGAGTGAGG AGAGGGTCAG TCCGCACCCC 
TGGATGGACA GCAAACGAGG AGAGGTAACC ACGTCTGAGG ATGCCACTAC CCCATCTTCA 
GCAGTGACCC CTTCTACTGC CTCTGCCTCA TCTCGACCCT TCATACCAGT CACAGATGAT 
CCTGGTGCTG CCAGCATCAT TGCTGAGACC ATGACCAAAA CCAAAGAGGA CTCTGAGAAC 
CAGAGTAAAG CCGTGGGTCC AGAGCCACAG TATCTGGATG AGTTCACCAG TCTCCTGATC 
CCTGATGACA CGCGTGTGAT GGTGGACCTG TTAAAACTAG CTGTATCTCT GCGCTCTGGT 
GATAAAGGAA AGGAAGTGCT GTCTGCTGTT CTGTCTGGCA TGGGTACAGC CTGCCCACAG 
GTGGCGGATA TGCTTCTGGA GCTGTGTGTG ACTGAGTTGG AGGACGTGGC GACTGACTGT 
CAGAGTGGAC GGCTTTCCTC TCAACCTGTG GTGGTGGAAA GCAGCCATCC ATATATTGAC 
GATACATCCA CCAGCGGAAC TGTCAAAATA CCAGGTGCTG AGGGTTTGCG GGTTGAGTTT 
GATCGCCAGT GCTCAACAGA AAGGAGACAT GACCCTCTTA CCATCATGGA TGGAGCCAAT 
AGAATTGTGT CTGTTCGATC AGGTCGTGAG TGGTCTGATT GGTCCAGTGA ATTGAGAATT 
CCAGGAGACG AGCTCAAATG GAAGTTCACC AGTGACGGCT CTGTGAACGG CTGGGGCTGG 
CGCTTCACAG TCTATCCCAT CATGCCTGCG GCTGGTCCTA AAGATCTTCT GTCAGACCGC 
TGCATTTTGT CTTGCCCTTC AATGGATCTA GTAACATGTC TGCTAGACTT TCGTCTGAAC 
TTTGCCTCAA ACCGCAGCAT TGTGCCCAGA TTAGCAGCCT CACTTGCTGC TTGTGCGCAG 
CTCAGCGCCT TGGCGGCTGG CCATCGTATG TGGGCTCTGC AGAGGTTACG CAAGCTCTTA 
ACTACTGAAT ACGGCCAATC CATTAACATC AACCGCCTCT TAGGAGACAC AGAAGGAGAG 
GCTCGCTCTA TGAGTTTTAC AGGAAGTGCA TTAGCTGCTC TGGTGAAAGG ACTTCCTGAG 
GCATTACAGA GACAGTATGA GTACGAGGAG CCCATAGTTA GGGGTGGCAA ACAGTTACTC 
CACAGCCCGT TCTTCAAGGT GTTGGTGGCG CTGGCGTGTG ATCTGGAGTT GGACACTCTT 
CCATGTTGTG CTGAGACACA CAAGTGGGCG TGGTTTCGAC GCTATTGCAC CGCCTCCAGA 
GTGTCAGTGG CTCTGGACAA GAGGAGCCCT TTGCCCCGCC CTTTTCTGGA TGAGGTAGTG 
GCAAAGAAAA TCCGTGAGCT GATGGCAGAT CATGAGAACA TGAACACGCT TCATGAGAGC 
CATGACTTGT TTAAGAGGGA GCAGGATGAG CAGTTGGTTC AGTGGATGAA CAGACGACCA 
GATGATTGGA CACTCTCTGC GGGTGGCAGT GGTACAATAT ACGGCTGGGG GCACAATCAC 
CGGGGTCAGT TAGGTGGCAT TGAAGGAGCC AAAGTAAAGG TTCCCACACC AACAGAAGCT 
CTAGCCACAC TGCGGCCAGT TCAGCTTATT GGAGGAGAGC AAACACTGTT TGCCGTCACT 
GCTGATGGGA AGCTGTATGC CACAGGATAT GGTGCCGGTG GTCGACTGGG AATAGGAGGG 
ACAGAGTCCG TCTCCACTCC GACCCTGCTG GAGTCCATCC AGCATGTTTT CATCAGAAAG 
GTGGCTGTGA ACTCTGGAGG GAAACACTGT TTGGCTCTTT CCTCTGAAGG AGAGCTGTAC 
TCCTGGGGGG AGGCCGAAGA TGGAAAACTG GGCCATGGAA ACAGAAGCCC ATGTGATCGT 
CCACGCGTGA TTGAGTCTCT CAGAGGGGTG GAAGTGGTGG ACATTGCTGC CGGTGGGGCT 
CACAGTGCCT GCATTACTGC CAGTGGAGAG CTCTACACAT GGGGCAAAGG TCGCTATGGG 
CGCCTGGGTC ATGGAGACAG CGAAGACCAA CTTAAACCAA AACTGGTGGA GGCTCTTCAG 
GGTCACCGGG TGATAGATGT GGCATGTGGC AGTGGAGATG CACAGACCCT CTGTCTGACT 
GATGATGACA TGGTATGGTC GTGGGGCGAT GGAGACTATG GCAAATTGGG CCGAGGAGGT 
AGTGATGGAT GTAAAGTGCC GATGAAGATC GATTCATTAA CCGGATTGGG TGTTGTGAAA 
GTGGAATGTG GCTCTCAGTT TTCTGTGGCC CTTACCAAGT CTGGAGCAGT TTACACATGG 
GGGAAGGGTG ACTATCACAG ACTGGGACAC GGCTCAGATG ATCATGTTCG CAGACCCCGG 
CAGGTCCAGG GTTTACAAGG CAAAAAGGTC ATCGCAATAG CAACTGGTTC CCTTCACTGC 
GTTTGCTGCA CGGAGGACGG GGAAGTGTAC ACATGGGGTG ACAATGATGA GGGTCAGCTT 
GGGGACGGTA CGACCAATGC TATTCAGAGA CCCAGGCTGG TGGCAGCGCT GCAGGGCAAG 
AAGATTAATC GTGTGGCCTG TGGTTCAGCA CATACACTTG CCTGGTCCAC TAGTAAACCA 
ACCAATGCTG GAAAACTCCC TGCTCAGGTT CCAATGGAGT ACAACCACCT GCAAGAAATC 
GCCATCATCG GCCTGAGGAA TCGGTTACTG CTACTGCACC ACATCTCTGA GCTCTTCTGC 
CCCTGCATCC CAATGTTTGA CTTAGAAGGA CGCCTGGGTG AAACTGGACA TGGTGTGTCT 
GTTGGCTTTG ACACGCTCCG TGGAATCCTC ATATCACAGG GCAAGGAGGC AGCATTCCGC 
AAAGTGGTGC AGGCTACCAT GGTCAGAGAC AGGCAGCATG GGCCAGTGGT TGAGCTGAAC 
AGGATACAGG TCAAGCGTTC ACGTAGTAAA GGAGGTCTGG CAGGGCCGGA TGGCACCAAG 
TCAGTATTTG GGCAGATGTG TGCTAAGATG AGTTCTTTCA GTCCAGACAG TCTGCTGCTG 
CCACACCGCG TCTGGAAGGT CAAGTTTGTG GGTGAGTCGG TGGATGACTG TGGTGGCGGA 
TACAGTGAAT CTATCGCAGA AATGTGCGAG GAGCTTCAGA ATGGCCTCAC TCCTCTGCTC 
ATCGTCACTC CTAATGGCAG AGACGAGTCA GGAGCGAACA GAGATTGCTT TCTGCTCAAC 
CCAGCTGCCA AATCCCCTCT GCACATCAGC ATGTTCCGCT TCCTAGGGGT GCTCCTGGGC 
ATAGCGATCC GAACAGGAAG TCCTCTTAGT CTGTATCTGG CCGAACCTGT GTGGAAACAG 
CTGGCAGGCA TGAACCTGAC CATTGCTGAT CTCAGTGAGG TGGACAAGGA CTTCATCCCC 
GGTCTGATGT ATATTAGAGA TAACGAGGCG TCTGCGGAGG AGTTTGAGGC GATGCCTCTT 
CCCTTCACTG TGCCCAGTGC CTGCGGACAA GAAGTCCAGC TCAGCTCCAA ACATTCCCAC 
ATCAGCCTTG AAAACCGCAG TGAATATGTG CGGCTAGCAA TAAATTACAG GCTGCATGAG 
TTTGATGAGC AGGTGTCTGC TGTAAGAGAA GGTATGGCGC GTGTGGTTCC GGTACCGCTC 
CTCTCTCTAT TTACCGGCTA TGAGCTGGAG ACCATGGTGT GCGGAAGTCC GGACATCCCT 
CTGCATCTGC TCAAATCAGT GGCCACATAT AAAGGAGTAG AGCCCACTGC ACCGCTCATC 
CAGTGGTTCT GGGAGGTGAT GGAGTCTTTC TCTAACACAG AACGCTCGCT CTTCCTCAGG 
TTCGTCTGGG GCCGCACGCG TCTTCCCAGG ACCATCGCAG ACTTCCGTGG ACGAGACTTT 
GTAGTGCAGG TGCTGGATAA ATATAACCCT CCCGACCACT TCCTCCCCGA GTCCTACACC 
TGCTTCTTCC TGCTCAAGCT GCCTCGCTAC TCCTGCAAGC TAGTCTTGGA GGAGAAACTC 
AAATACGCCA TTCACTTCTG CAAGTCCATC GACACTGATG ACTACGCACG GATCGCTCTG 
AGCGGAGAAC CGGCCGCAGA CGACAGCAGT GATGACTCGG ACAATGAAGA CGCAGACTCA 
TTCGCTTCTG ACTCCACACA GGATTACCTG ACAGGCCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC2 ( XM_009302643.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009300918.2 Danio rerio HECT and RLD domain containing E3 ubiquitin protein ligase 2 (herc2), transcript variant X2, mRNA.

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CloneID	ODa59482
Clone ID Related Accession (Same CDS sequence)	XM_009302643.2
Accession Version	XM_009302643.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 14499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase HERC2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009302643.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTCTC CATCATTTTC CCTGAATGCC CAGCTGCGCT TCCATGATAA ATGGCTGAAG 
ATCGACTTGC AGCGCGCCTT CTCTCATGAA GGACTGAGCG AGATGTGGAA CGAGATGGTG 
AAGGATGAAG AGATCGTCTT TGACAGAGAA GAGTCTGCCC ATCCAGAGGA CTGTGCTGAT 
TGCACCCAAA AGAAAGATGA CACCAATGAT AAAGAAAAGA AGGAAGAGGA GGAGGCAGCA 
GCAACGGCGG CTTCATCTTT CCATCACTCC ATCATTGAAA TGTGGGACTG GGGGAGGCAG 
CCAGAAACCA GCGAGCTGCG GGACTGTCTG ATGGCTTTGG TAGACGACCA ACAGAAGCTG 
TCTTCTCAGA CGGCTAAGAG CACACTCTCA GCCCTGCGTC TAAGCCAGAG ACTGGTGATC 
CTGGAGCGCT ACCTCATCTC GCTCACTCAC AGCATGATGG AGGAGAAGTA CAAAGTACGG 
TGGAAGAGCC CTCCCAGTCC TCCACTCTCT GCTGTCGACA ACAAAAGCGC TCGGCCAGTT 
AGTAAAGGGG TGGAAGGTTT GGCCAGGGTG GGCTCAAGGG CAGCTCTTTC CTTTGCCTTT 
GCATTTCTCC GCCGAGCCTG GAGATCAGGA GATGATGCAG ACCTGTGCAG TGAGTTACTG 
CAGGAGTCTC TGGATGCTCT GCGGGCTCTT CCAGAAGCTA CTCTGTTTGA TGAGGGCACT 
GTATCTCCTG TTTGGCTGGA GGTGGTTGAG AGGGCTACTA AATTTCTAAG TGACATCCAT 
GGAAGTGCCA GTGGAAAAGC ACCCAGTAAC ATTCCCTTGC AGGATCAGCA CTTGGCCCTT 
GCCATCCTGC TGGAGCTCGC AGTGCAACGA GGAACTCTCA GTCAGCTGCT GTCTGCAGTT 
CTGCTGCTTC TGAGGCTCTG GGACAGTGGA ACTAGAGAGA TGGATAATGA GCGCTCCACC 
CAAGGCACCA GCGCCCCGTT ACTGCCCCTC CTGCAGCGCT TCCAGAACAT CCACAGCAGT 
AAAGAGGAGC CTGTTCCTGA AGAGGAGATT GAGATCCTTT CGGCCCCTCT GAGCCCGAAT 
GAGAGTTTTC TGCGCTACCT TACACTGCCT CAAGATAATG ACCTGGCCAT TGATTTGCGG 
CAGACAGCTG TAGTCATCAT GGCACATTTG GACCGCCTGG CAGCCCCATG CAGCCCCCCG 
CAGTGCAGCT CACCCACTTC TAACAAGGGG ACACTGCAGG AGGTGATTGG TTGGGGTCTG 
CTTGGATGGA AGCCTTATGC TAATGTGAAT GGACCAATTC AGTGCAAGGC ACTATCAAAT 
CTGGGTGTCA CGCAGATTGT CTGCTCAGAG AAGGGTTTCC TCATCCTGAC CATCACTGGT 
GCTGTCTACA CACAAAACTA CAAAAGTACT ACACTAGCAC CTATGCTAGT CCATGCACTG 
ACGTCCAGGA AAATAATGAA GCTTGCAGCT CATCCTGATG GCCAGCATTA TTTGGCTCTT 
TCAGTCAATG GAGAGGTTTT CTCCTGGGGT TGTGGAGATG GAGGCAGGCT AGGACATGGA 
GACACTACGT ATTTAGAGGA GCCCACTATG ATTTCAGTAT TCTCAGGGAG GCCGGTAGGG 
AAGCAGGTTG TACATATTGC CTGTGGCAGC ACATACAGCG CAGCCATCAC AGCAGATGGA 
GAGCTTCACA CATGGGGCCG AGGAAACTAC GGCAGACTGG GCCATGGCTC CAGTGAGGAC 
CAGACCACTC CCATGCTAGT AACTGCACTG AAGGGGCTGA AGGTGATCGA TGTGGCCTGT 
GGAAGCGGAG ATGCTCAGAC GCTTGCTGTG ACTGAGAATG GTCAGGTATG GTCATGGGGT 
GATGGAGATT ATGGGAAACT GGGCCGAGGG GGCAGTGATG GATGCAAAAC ACCAAAACTA 
GTGGAGAAGC TCCAGGATCT GGATATTGTG AAGGTGTGCT GTGGGAGCCA GTTCTCCGTA 
GCGCTCACTA AAGATGGACA AGTTTACACC TGGGGCAAAG GAGACAATCA ACGACTTGGA 
CATGGCACTG ATGAACATGT CCGCTATCCA AAACTACTTG ACAGTCTGCA AGGCAAGAGG 
GTGGTGGACA TAGCGGTTGG CTCCACACAC TGCCTGGCCC TTACAGAAGA AGGAGAGGTG 
CACAGCTGGG GCAGTAATGA CAAACTACAG CACTTTGACA CACTTTTCTC CAATAAGAAG 
CAGCCCAAAG CCTTGCCAGG CCTCAACTCC AAACACATAG TAGGCATCTC CTGCGGTCCT 
GGCCAGAGTT TTTCATGGTC ATCCTGCTCT GAGTGGTCTG TGGGTCTCCG GGTACCCTTC 
GTGGTAGACG TGTGCACTAT GACCTTTGAC CAGCTGGACC TGCTTCTGCG CCAGGTTAGT 
GAGGGCATGG ACGGCAGCTC TGATTGGCCA CCACCACAGG AGAAAGAATG CATGGTCGTG 
GCAGCTCTCA ACCTGCTCAG ACTACAGCTT CATGCAGCCA TCAGTAATCA GGTGGATCCC 
GAGGAGCTGG GTCTGGGTCT GGGCAGTGTG CTGCTTAATA ACCTCAAGCA GACGGTGGTG 
ACTCTGGCCA GCAACGGCGG GGTGCTAAAC ACTGTGCAGG CTGCCGCCCA GACCGTGCTC 
CAGAGTGGCT GGTCAGTGCT ACTCCCCACT GCAGAGGAAC GAGCAAGAGC CCTGTCCTCG 
CTGCTGCCCA ATGCAGCATC TGCTAATGAG ATGAGTGTAA GTCCTGGCCG TCGCTTCATG 
ATCGACCTCT TGGTTAGTAG TCTGATGGCA GATGGAGGGT TGGAGTCTGC TTTGAATGCT 
GCCATCACGG CCGAGATCCA GGATATTGAG GCAAAGAAAG AGGCTCAGAA AGAGAAGGAA 
ATCGATGAGC AGGAAGCCAG CGCCTCTTCT TTGCATCGCT GTCGCACCAC ACTGGACAAA 
GACCTGATCA ACACCGGCAT CTATGAGTCT GCCAACAAAC AGAGCCTCCC ATTGGTCCAG 
CTTGTCCAAC AGCTGCTCAG GAACATTGCT TCACAGACTA TAGCCCGTCT TAAGGATGTG 
GCCCGACGCA TCTCAAATTG TCAAGACACC GAGCAGGTCA GCAAGGAGCG CTCAGCCTCT 
CTGGACCTGC TGCTGCGCTT TCAGAGGCTT TTGGTCTGCA AATTGTATGT CGGAGTCAAT 
GGCACTGTGG GTGTGGGAGG ATACACTTTG AGTCACTCAG AAATTCTGGG AGTTGGCTCT 
CTCCTGAAGA AGTATATGGC TTTGCTTTGC ACACACATCG GTGATATCCT TCCTGTGGCC 
ACCAGCATTG CCCTCAACAG CCATCGTCAC TTTGCAGAGG TGGCTCGTGT CCTTGAGGGA 
GATCTTACAG GTGTTCTGCT ACCAGAGCTC ATTGTGTCCA TTGTGCTGCT GCTGACCCAT 
GATGCTGGAC TAATGCAAGA GACTGGATCC ATTCCTCTGC TCGCTGCTCT TCTGGAGCAC 
TTGGACCGTT TTAACTATCT TGCACCTGGT ACAGAAAGAG ATGACAATGA GGATCTTGCC 
TGGCCTGGAC TCATGGGTTT CTTTTTCAAT GGACCGAGCT CAAAGAACAA CGAGGAGGTG 
TCACTTATCC GCAAAGCAGA CCTGGAGAAT CACAACAAAG ATGGCGGCTT TTGGACTGTA 
ATTGAAGGGA AGGTCTATGA CATAAAGGAC TTTCAAGCTC AGACTCTAAC TGGCAACAGC 
ATCCTTGCCC AGTTTGCAGG AGAAGACCCT GTGGTGGCAT TAGAGGCTGC CCTCCAATTT 
GAAGACACCA GAGAGTCCAT GCAAGCTTTC TGTGTGGGAC AGTATATGGA GCCCAACCAA 
GAAATGGTGA CAACGCCTGA CCTGAGCAGC CTGTCCTCTC CTCTTATAGA TGCTGAGAGG 
AACCTTGGTC TGCTTCTGGG GCTACATGCC TCATATCTGG CTATGAGCAC CCCTCTCTCC 
CGCATAGAGT TAGAATGTGC CAAATGGCTC CAGTCATCTA TTTTCTTCGG TGGACTTCAG 
ACCAGTCAGA TCCACTATAA TTATAACGAG GAGAAGGATG AGGACCACTG CAGCTCTCCA 
GGGACCACCA CACCAGACAA AGCCAAACTG TACTGCCGCA GAATCACCCT GAGTGATCAC 
GCCCAGCCCT TCCTGCAGGC CATCGCTGAC AACACCACCC AGGATCACAC TGTGAAGGAC 
TTTTTATGCC AAATCGAGCG GTGCTGTAAA CAGTACCATC TCATCACTCC CATCACTTTT 
CCACCTGAAC ATCCTGTGGA GGAAGTGGGT CGCCTGCTGC TCTGCTGCCT TCTGAAGCAT 
CAGGATCTTG GTCATGTCGC CCTCTCCCTA GTTGATCAAT GTGCCTTGGG TATGGACCAG 
GGGAAACAGA GGTCCCTCCC GAAATCTGTG ATTGATGTGT GTCGAATGGT GTATCAGGCC 
AAGTGTTCGT TGATAAAAAC ACACCAGGAA CAGGGTCGTT CTTATAAAGA GGTGTGCGCT 
CCTGTTATTG AGAGGTTGCG TTTCCTGTTT AATGAGCTAC GACCTGCCGT CAGTAATGAC 
TTGTCCATTG TGTCCAAACT CAAACTGCTG AGCTCTCAGC CTCGCTGGAA GAGGATCACA 
CAGAAACTGA TCAGAGACCG CCGCAAAAAG AGAGTGCCTA AAAAGTCGGA GTCAGCTGAT 
CTGGAGGAGT GCAAGATGGA GAGGGAAGGG ACCAATGAGG AACCACCTGC TCCTCTAAGC 
CCCGCTCCTG TTGACAAGAA GCAAACAACT GTCAAATCTG CAAAGCAGGA TAAGTGGCAG 
CCTCTGCTAA ACACCATCGG CAGTGTTCAG AAGTACCGCT GGTTAAGGCA CAGCGTTCAG 
GGCTCCTTCT CTCAGTCCAG CCTCATGAGC ACCATCGTTG AGTTTTCTTT GAAAGAGGAG 
CCGCTGGATG TGGAGAAGAT CAGAAAGTGC CTCCTTAAAC AGCTGGAGAG AGCTGAGGTC 
CGCCTGGAGG GTATTGACAC TATGTTGAAG CTGGCATCTA AAAACTTCCT CCTGCCCTCT 
GTTCAGTATG CCATGCTTTG TGGCTGGCAG AGAGTCATAC CTGAAGGGAC CAATATTGGA 
GAGCCATTGG CTGACTGTCT AAAGGATGTA GATCTCATTC CTCCATTCAA CCGCATGCTA 
CTAGAGGTGA CGTTTGGGAA GCTCTATGCC TGGTCCATAC AGAACGTCCG CAACATCTTG 
TTGGAGGCTA GTGTCAAGTT CAAGGAACTG GGTGTGCAAC CAGTGCCTCT CCAGACAATC 
ACTAATGAGA ACCCAGCAGG CCCCAGTCTG GGTACCATCC CTCAGGCACG CTTCCTGCTG 
GCTATGCTCC ATATGCTCAC TCTCAAACAC GGAGCCAACA GCCTCAGCCT CCTGCTGAAC 
TCTGGCATCC TCGCTCTCAC CCAGAGTGTC CTCCGACTCA TTGGTCCTAG CTGTGATAGC 
AGTGAGGAGG AGCTGACCTC TGTAGCTCAT GGAGGCTCTG CCACAGTTCT GGAAGAGTCC 
AGAAAGGAGA CTGCCCCGAC CCCTCTGCCC GCTTCCGGCC CTGAGCTGGC AGCTATGATG 
AAGATCAGCA CAAGGGTGAT GAGAGGTCCT GACTGGAAGT GGGGGGACCA GGATGGCCCA 
TCACCAGGCC TGGGTCGTGT GATTGGGGAG TTGGGAGAAG ATGGTTGGAT AAGAGTCCAA 
TGGGACACCG GCAGCACCAA CTCCTACAGG ATGGGAAAAG AGGGCAAGTA TGACCTTAAA 
CTGGCTGAGC CGCCACCTGC CACCCAACCA GCCACTGAAG ACTCTGACAC GGAGGATGAC 
ACAGAAGGGG AGGTGATGGA AAAGTGTGCC CACCCCACGG CAATCATGCT GACTAGTACA 
GTAAATCTGT TGAAGACACT GTCACTGTCT TCTGGGATTC ATGCAGAGGT GATGCAGGTC 
GATGCGGTCA GTACTCTGTG CGGGCTACTC AGGATACTGG TGGAGAGTGG AGCCAACGAC 
AAGACTGGTT GCTCCTCTCA CAAGCTGGTG TCCCGAGAGC AGCACAGGAG CTGGTGCACG 
CTGGGATTTA TTCGCAGTAT AGCTCTTATG CCTCAGATGT GCAGTACCCT GAGCTCCTCT 
GCCTGGATCA ATCTGCTTAT ACGCATCGTG GAGGGTCATC AGTCCTTCAC TGCAATCACA 
CTACAGAGAC AGATTCTTGC CCTGCGGCTT CTACAGGCAG TATTGCCCTC CTGGGATAAG 
ACTGAACGCT CTCAGGATAT GAAGTTCTTG GTGGAGAAAC TGTTTGGGTT CCTCGGCAGC 
TTGCTCAGCA CTTGTTCTTC AGATCTGCCT CTTCTTAGGG AAGGTTCAAT GCGTAGGAGG 
AAGTCCCGCC CCCAGGCCTC ACTGACAGCC ACTCATAGCA GTACTTTGGC AGAGGAAATA 
GTGACAGTAT TGCGCACGCT TCACTCCCTC AGCCAATGGA ATGCCCTGAT TAATGAATAC 
ATTAACTCTC AGCTCAGTGG AATCGGGGAT GTCATGGCAG GCCGACACAC TGAAGTGGCT 
TTGCTTGAAG ACTATCTCCC CAATGCGGAA GCAGCAGCGG TGGGCAGTCT GATGGCTGTT 
TTGGCAGTCA TCGGGGGGAT TGATGGACGT CTGCGTCTGG GTGGACGTGT GGTCCATGAG 
GAGTATGGAG AAGGCACAGT GACTCGCATC ACACCTAAAG GACGAATCAC TGTTCAGTTT 
CATGAGATGA GGAACTGCAA AGTGTGCCTG CTCAGTCAGC TCAAACCGCT TCACATGATT 
CCATTCAGCG TTCAGAATCT GCCCTTCGCT GAGCCCATGC TAATGGTGTG GGCCCAGCTA 
GTCAACTTGG CTGGGAGCAA GCTGGAGAAA CAACGCATGA AGAAATCTCT TAGTAGAGGA 
CTTACAGCGG ATCAGGTAGA TGTACATTTG TTGCGCTGTC AGCAGCTCAG GCTGTACATC 
TTGAAGGCTG CACGAGCTCT ACTCTCCCAT CAGGACAAAC TCAGACAGAT CCTTTCTCAG 
GCGGCCGTGG TTGATGTCAG CCCCAATCCT ACAGAGGATC AGTGCGCGTC GTCTCCTGAT 
GTAGGAGATT TGTCTCCTGA GGGTCCTCAG CCTCCTCTCA TCCTCCTTCA GCAGTTGCTG 
TCAGCATCCA CTCAACCTTC ACCCATCAAA GCCATATTCC ACCGCCAGGA GTTAGAGGCA 
GCGGCTCTGG CTGTGTGTCA GTACCTGGCG GTTGAATCCA CACACCCGTC ATCGCCTCTG 
TTTGAGGACA GCAGCTCTAG CGAAGCCACT ACACCAGTCA CTGTGCAGCA CATGCGTCCA 
CCCAAACAAA AGAAACACAA GACGTCACCT GTGCCCCCTG CTCCCATCGT CCTGCAGCTG 
ATGGAGATGG GCTTTCAAAG GAAAAACATC GAGTTTGCTC TTAAATCACT GTCTGGAACC 
ACAGGTGCTT CTGGAGTGCC AGGTGTCGAG GCATTGGTGG GCTGGTTGTT GGACCATCCA 
GATGTTCTCA TTACAGATCT GTCGGATGCA GAGACTGTAT CAGATGAATA CTCTGATGAA 
GAGGTGCTGG AAGAGCTTGA GGAGCCTGAG CCTGCCTTTC CTGTGCCTCC TGGGGTTGTG 
GTGACTGAGA GTCAGACGTT TAAAAAGAGG TCGGACTTTC AGAGCAATGA CGATTATGCT 
GTTTACGTGA GAGAGAACAT TCAGGTGGGC ATGATGGTAC GGTGTTGCAG GACATATGAG 
GAAGTGTATG AAGGAGATGT GGGGAAAGTG ATCAAGCTTG ACAGAGACGG CCTGCATGAC 
CTGAATGTTC AGTGTGACTG GCAGCAGAAA GGAGGAACTT ACTGGGTCCG TTACATCCAC 
ACTGAGCTGC TGGGGTTTCC CCCTCAGAGT TCTCCATCTC ATATAAAGAT TGGTGATAAA 
GTGCGAGTGA AGCCCTCGGT CACAACACCA AAATACAAGT GGGGGTCAGT CACTCATAGG 
AGTGTGGGTG TGGTCAAAGC CTTCAGTGCC AATGGGAAGG ATGTCATTGT GGACTTTCCT 
CAGCAGTCGC ACTGGACTGG ACTGCTGTCA GAGATGGAGC TTGTGCCCAG CGTACATCCT 
GGTGTCAGGT GTGACGGATG TCAGATGTTC CCTATAAATG GCCCAAGGTT TAAGTGTCGG 
AGCTGTGATG ACTTTGACTT TTGTGAGAGC TGCTTCAAGA CCCGTAAACA CAATCCTAGG 
CATTCATTTG GGAGGATAAA CGAACCAGGT CAGTCACCCA CCTTTAGTGG GCGCTCAGGA 
AAGCAGCTGA AAAGACATCA CAGCAGTCAG CGTGGCATGC TGATCGACGA GTGGTCAAGA 
GTGGTGAAGA ACCTCAGCGT ATCGTCTTCT GTTAACCAGG CGTCTCGACT CATCGACTGT 
GGAGAGCAAT GCTGGCAGTC CTCAGGCTCT CAGGGCAAGC ACTGGATCAG ATTGGAGCTT 
TTCCCAGACA TGTTAGTCCA CAAACTGAAA ATGATTGTAG ATCCAGCTGA CAGCAGCTAC 
ATGCCTTCAT TGGTGGTGGT ATCAGGTGGA ACCTCACTCA ACAATTTGAT CGAGTTGAAG 
ACCATCAACA TCAATCCCAC TGACACTGTC GTCTCTCTAC TCAATGACTG CACAGAGTAT 
CACAGGTACA TTGAGGTTGC TATAAAGCAG TGTCGAAGTT CAGGTATTGA CTGTAAGATT 
CATGGCTTGA GTGCTGTGGG ACGTGTCCGT GCCGAGGATG AAGATCTTGC AACTGTACCT 
TTCCTGGCAT CTGACAACGA GGAAGAGGAA GATGAGAAGA CCGCGACTGG AAGTATGTCT 
CGAAAGAGAT TGTCAGGCCT CGAGTCTGCA GCCACTATCA GAACCAAAGT CTTTGTCTGG 
GGTCTTAATG ATAAGGACCA ACTTGGAGGA CTTAAGGGGT CCAAGATCAA AGTCCCTTCA 
TTCTCAGAGA CTCTGTCTGC TCTGAATGTA GTGCAGGTGG CTGGTGGCTC TAAAAGTCTT 
TTTGCTGTGA CTGCTGAAGG TAAAGTATAT GCCTGTGGAG AGGCCACCAA TGGAAGACTT 
GGTTTAGGCC TCTCCAGTGG AACCATCCCA ATTCCCAGAC AGATATCAGC TCTCAGCAAC 
TATGTGGTCA AGAAGGTTGC TGTGCATTCA GGTGGACGGC ATGCCATGGC ACTTACCGTG 
GATGGGAAAG TATTCTCTTG GGGTGAAGGA GACGATGGCA AGCTTGGACA CTTCAGCCGA 
ATGAACTGTG ATAAGCCAAG GCTGATCGAG GCATTGAAAA CCAAACGCAT TCGAGATATT 
GCGTGTGGCA GCTCTCACAG TGCCGCCATC ACCTCCAGTG GAGAGCTGTA CAGCTGGGGT 
CTGGGGGAAT ATGGCCGTCT GGGCCATGGG GACAACACCA CCCAACTCAG ACCCAAACTG 
GTCAAGGTGC TTTTAGGCCA TCGCGTTGTC CAGGTAGCTT GTGGAAGTAG AGATGCCCAA 
ACTCTTGCAC TGACAGATGA AGGAATGGTG TTCTCATGGG GAGATGGAGA CTTTGGGAAA 
CTGGGCCGTG GTGGCAGCGA GGGATGTAAC ATTCCTCAAA ACATTGAGCG TCTGAATGGA 
CAGGGGGTCT GTCAAATTGA ATGTGGAGCT CAATTCTCCC TCGCCCTCAC CAAATCTGGA 
GTTGTCTGGA CCTGGGGTAA AGGGGATTAC TTCAGACTGG GTCACGGCAC AGATGTTCAT 
GTCCGCAAAC CCCAGATGGT TGAAGGACTT AGGGGGAAGA AGATTGTTCA TGTGGCCGTT 
GGTGCTCTGC ACTGCCTGGC TGTCACCGAG ACTGGACAGG TGTTCGCGTG GGGGGATAAT 
GACCATGGGC AGCAGGGGAA TGGCACAACC ACAGTCAACA GGAAGCCCAC ATTGGTGCAG 
GGTCTAGAGG GTCAGAAAAT TACTCGTGTG GCCTGCGGTT CTTCTCATAG TGTTGCCTGG 
ACCACAGTAG ATGTCACTAC ACCCTCCGTC CATGAGCCTG TGCTCTTCCA GACTGCTAGA 
GACTCTCTGG GAGCCTCTTA CCTTGGTGTG CCATCAGATA GTGACCCGTC TTTACTGAGC 
AATAAGCTAA ATGGCGTAAA CAATGTGAAG CCCAACAGGC CATCTCTGGC AAAGATCCTT 
CTCTCTCTGG ATGGTAATCT GGCCAAACAG CAGGCCCTAT CCCATGTGCT GTCTTCCCTG 
CAGATCATGT ATGCCAGAGA TGCAGTGGTG GGTGCATTAA TGCCATCCTG TATGATGCCT 
CTGGAGTGTC CCTCCAGCTC TCCGGTGCCC CCATCAGACT GTTCATCAGC TTCCAGCCCC 
TGTGAGGCTG AGGGTCCTGC CCTGCCCTCA GAGAGTGAGG AGAGGGTCAG TCCGCACCCC 
TGGATGGACA GCAAACGAGG AGAGGTAACC ACGTCTGAGG ATGCCACTAC CCCATCTTCA 
GCAGTGACCC CTTCTACTGC CTCTGCCTCA TCTCGACCCT TCATACCAGT CACAGATGAT 
CCTGGTGCTG CCAGCATCAT TGCTGAGACC ATGACCAAAA CCAAAGAGGA CTCTGAGAAC 
CAGAGTAAAG CCGTGGGTCC AGAGCCACAG TATCTGGATG AGTTCACCAG TCTCCTGATC 
CCTGATGACA CGCGTGTGAT GGTGGACCTG TTAAAACTAG CTGTATCTCT GCGCTCTGGT 
GATAAAGGAA AGGAAGTGCT GTCTGCTGTT CTGTCTGGCA TGGGTACAGC CTGCCCACAG 
GTGGCGGATA TGCTTCTGGA GCTGTGTGTG ACTGAGTTGG AGGACGTGGC GACTGACTGT 
CAGAGTGGAC GGCTTTCCTC TCAACCTGTG GTGGTGGAAA GCAGCCATCC ATATATTGAC 
GATACATCCA CCAGCGGAAC TGTCAAAATA CCAGGTGCTG AGGGTTTGCG GGTTGAGTTT 
GATCGCCAGT GCTCAACAGA AAGGAGACAT GACCCTCTTA CCATCATGGA TGGAGCCAAT 
AGAATTGTGT CTGTTCGATC AGGTCGTGAG TGGTCTGATT GGTCCAGTGA ATTGAGAATT 
CCAGGAGACG AGCTCAAATG GAAGTTCACC AGTGACGGCT CTGTGAACGG CTGGGGCTGG 
CGCTTCACAG TCTATCCCAT CATGCCTGCG GCTGGTCCTA AAGATCTTCT GTCAGACCGC 
TGCATTTTGT CTTGCCCTTC AATGGATCTA GTAACATGTC TGCTAGACTT TCGTCTGAAC 
TTTGCCTCAA ACCGCAGCAT TGTGCCCAGA TTAGCAGCCT CACTTGCTGC TTGTGCGCAG 
CTCAGCGCCT TGGCGGCTGG CCATCGTATG TGGGCTCTGC AGAGGTTACG CAAGCTCTTA 
ACTACTGAAT ACGGCCAATC CATTAACATC AACCGCCTCT TAGGAGACAC AGAAGGAGAG 
GCTCGCTCTA TGAGTTTTAC AGGAAGTGCA TTAGCTGCTC TGGTGAAAGG ACTTCCTGAG 
GCATTACAGA GACAGTATGA GTACGAGGAG CCCATAGTTA GGGGTGGCAA ACAGTTACTC 
CACAGCCCGT TCTTCAAGGT GTTGGTGGCG CTGGCGTGTG ATCTGGAGTT GGACACTCTT 
CCATGTTGTG CTGAGACACA CAAGTGGGCG TGGTTTCGAC GCTATTGCAC CGCCTCCAGA 
GTGTCAGTGG CTCTGGACAA GAGGAGCCCT TTGCCCCGCC CTTTTCTGGA TGAGGTGGCA 
AAGAAAATCC GTGAGCTGAT GGCAGATCAT GAGAACATGA ACACGCTTCA TGAGAGCCAT 
GACTTGTTTA AGAGGGAGCA GGATGAGCAG TTGGTTCAGT GGATGAACAG ACGACCAGAT 
GATTGGACAC TCTCTGCGGG TGGCAGTGGT ACAATATACG GCTGGGGGCA CAATCACCGG 
GGTCAGTTAG GTGGCATTGA AGGAGCCAAA GTAAAGGTTC CCACACCAAC AGAAGCTCTA 
GCCACACTGC GGCCAGTTCA GCTTATTGGA GGAGAGCAAA CACTGTTTGC CGTCACTGCT 
GATGGGAAGC TGTATGCCAC AGGATATGGT GCCGGTGGTC GACTGGGAAT AGGAGGGACA 
GAGTCCGTCT CCACTCCGAC CCTGCTGGAG TCCATCCAGC ATGTTTTCAT CAGAAAGGTG 
GCTGTGAACT CTGGAGGGAA ACACTGTTTG GCTCTTTCCT CTGAAGGAGA GCTGTACTCC 
TGGGGGGAGG CCGAAGATGG AAAACTGGGC CATGGAAACA GAAGCCCATG TGATCGTCCA 
CGCGTGATTG AGTCTCTCAG AGGGGTGGAA GTGGTGGACA TTGCTGCCGG TGGGGCTCAC 
AGTGCCTGCA TTACTGCCAG TGGAGAGCTC TACACATGGG GCAAAGGTCG CTATGGGCGC 
CTGGGTCATG GAGACAGCGA AGACCAACTT AAACCAAAAC TGGTGGAGGC TCTTCAGGGT 
CACCGGGTGA TAGATGTGGC ATGTGGCAGT GGAGATGCAC AGACCCTCTG TCTGACTGAT 
GATGACATGG TATGGTCGTG GGGCGATGGA GACTATGGCA AATTGGGCCG AGGAGGTAGT 
GATGGATGTA AAGTGCCGAT GAAGATCGAT TCATTAACCG GATTGGGTGT TGTGAAAGTG 
GAATGTGGCT CTCAGTTTTC TGTGGCCCTT ACCAAGTCTG GAGCAGTTTA CACATGGGGG 
AAGGGTGACT ATCACAGACT GGGACACGGC TCAGATGATC ATGTTCGCAG ACCCCGGCAG 
GTCCAGGGTT TACAAGGCAA AAAGGTCATC GCAATAGCAA CTGGTTCCCT TCACTGCGTT 
TGCTGCACGG AGGACGGGGA AGTGTACACA TGGGGTGACA ATGATGAGGG TCAGCTTGGG 
GACGGTACGA CCAATGCTAT TCAGAGACCC AGGCTGGTGG CAGCGCTGCA GGGCAAGAAG 
ATTAATCGTG TGGCCTGTGG TTCAGCACAT ACACTTGCCT GGTCCACTAG TAAACCAACC 
AATGCTGGAA AACTCCCTGC TCAGGTTCCA ATGGAGTACA ACCACCTGCA AGAAATCGCC 
ATCATCGGCC TGAGGAATCG GTTACTGCTA CTGCACCACA TCTCTGAGCT CTTCTGCCCC 
TGCATCCCAA TGTTTGACTT AGAAGGACGC CTGGGTGAAA CTGGACATGG TGTGTCTGTT 
GGCTTTGACA CGCTCCGTGG AATCCTCATA TCACAGGGCA AGGAGGCAGC ATTCCGCAAA 
GTGGTGCAGG CTACCATGGT CAGAGACAGG CAGCATGGGC CAGTGGTTGA GCTGAACAGG 
ATACAGGTCA AGCGTTCACG TAGTAAAGGA GGTCTGGCAG GGCCGGATGG CACCAAGTCA 
GTATTTGGGC AGATGTGTGC TAAGATGAGT TCTTTCAGTC CAGACAGTCT GCTGCTGCCA 
CACCGCGTCT GGAAGGTCAA GTTTGTGGGT GAGTCGGTGG ATGACTGTGG TGGCGGATAC 
AGTGAATCTA TCGCAGAAAT GTGCGAGGAG CTTCAGAATG GCCTCACTCC TCTGCTCATC 
GTCACTCCTA ATGGCAGAGA CGAGTCAGGA GCGAACAGAG ATTGCTTTCT GCTCAACCCA 
GCTGCCAAAT CCCCTCTGCA CATCAGCATG TTCCGCTTCC TAGGGGTGCT CCTGGGCATA 
GCGATCCGAA CAGGAAGTCC TCTTAGTCTG TATCTGGCCG AACCTGTGTG GAAACAGCTG 
GCAGGCATGA ACCTGACCAT TGCTGATCTC AGTGAGGTGG ACAAGGACTT CATCCCCGGT 
CTGATGTATA TTAGAGATAA CGAGGCGTCT GCGGAGGAGT TTGAGGCGAT GCCTCTTCCC 
TTCACTGTGC CCAGTGCCTG CGGACAAGAA GTCCAGCTCA GCTCCAAACA TTCCCACATC 
AGCCTTGAAA ACCGCAGTGA ATATGTGCGG CTAGCAATAA ATTACAGGCT GCATGAGTTT 
GATGAGCAGG TGTCTGCTGT AAGAGAAGGT ATGGCGCGTG TGGTTCCGGT ACCGCTCCTC 
TCTCTATTTA CCGGCTATGA GCTGGAGACC ATGGTGTGCG GAAGTCCGGA CATCCCTCTG 
CATCTGCTCA AATCAGTGGC CACATATAAA GGAGTAGAGC CCACTGCACC GCTCATCCAG 
TGGTTCTGGG AGGTGATGGA GTCTTTCTCT AACACAGAAC GCTCGCTCTT CCTCAGGTTC 
GTCTGGGGCC GCACGCGTCT TCCCAGGACC ATCGCAGACT TCCGTGGACG AGACTTTGTA 
GTGCAGGTGC TGGATAAATA TAACCCTCCC GACCACTTCC TCCCCGAGTC CTACACCTGC 
TTCTTCCTGC TCAAGCTGCC TCGCTACTCC TGCAAGCTAG TCTTGGAGGA GAAACTCAAA 
TACGCCATTC ACTTCTGCAA GTCCATCGAC ACTGATGACT ACGCACGGAT CGCTCTGAGC 
GGAGAACCGG CCGCAGACGA CAGCAGTGAT GACTCGGACA ATGAAGACGC AGACTCATTC 
GCTTCTGACT CCACACAGGA TTACCTGACA GGCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300918.2
CDS	144..14642
Translation	MPSPSFSLNAQLRFHDKWLKIDLQRAFSHEGLSEMWNEMVKDEEIVFDREESAHPEDCADCTQKKDDTNDKEKKEEEEAAATAASSFHHSIIEMWDWGRQPETSELRDCLMALVDDQQKLSSQTAKSTLSALRLSQRLVILERYLISLTHSMMEEKYKVRWKSPPSPPLSAVDNKSARPVSKGVEGLARVGSRAALSFAFAFLRRAWRSGDDADLCSELLQESLDALRALPEATLFDEGTVSPVWLEVVERATKFLSDIHGSASGKAPSNIPLQDQHLALAILLELAVQRGTLSQLLSAVLLLLRLWDSGTREMDNERSTQGTSAPLLPLLQRFQNIHSSKEEPVPEEEIEILSAPLSPNESFLRYLTLPQDNDLAIDLRQTAVVIMAHLDRLAAPCSPPQCSSPTSNKGTLQEVIGWGLLGWKPYANVNGPIQCKALSNLGVTQIVCSEKGFLILTITGAVYTQNYKSTTLAPMLVHALTSRKIMKLAAHPDGQHYLALSVNGEVFSWGCGDGGRLGHGDTTYLEEPTMISVFSGRPVGKQVVHIACGSTYSAAITADGELHTWGRGNYGRLGHGSSEDQTTPMLVTALKGLKVIDVACGSGDAQTLAVTENGQVWSWGDGDYGKLGRGGSDGCKTPKLVEKLQDLDIVKVCCGSQFSVALTKDGQVYTWGKGDNQRLGHGTDEHVRYPKLLDSLQGKRVVDIAVGSTHCLALTEEGEVHSWGSNDKLQHFDTLFSNKKQPKALPGLNSKHIVGISCGPGQSFSWSSCSEWSVGLRVPFVVDVCTMTFDQLDLLLRQVSEGMDGSSDWPPPQEKECMVVAALNLLRLQLHAAISNQVDPEELGLGLGSVLLNNLKQTVVTLASNGGVLNTVQAAAQTVLQSGWSVLLPTAEERARALSSLLPNAASANEMSVSPGRRFMIDLLVSSLMADGGLESALNAAITAEIQDIEAKKEAQKEKEIDEQEASASSLHRCRTTLDKDLINTGIYESANKQSLPLVQLVQQLLRNIASQTIARLKDVARRISNCQDTEQVSKERSASLDLLLRFQRLLVCKLYVGVNGTVGVGGYTLSHSEILGVGSLLKKYMALLCTHIGDILPVATSIALNSHRHFAEVARVLEGDLTGVLLPELIVSIVLLLTHDAGLMQETGSIPLLAALLEHLDRFNYLAPGTERDDNEDLAWPGLMGFFFNGPSSKNNEEVSLIRKADLENHNKDGGFWTVIEGKVYDIKDFQAQTLTGNSILAQFAGEDPVVALEAALQFEDTRESMQAFCVGQYMEPNQEMVTTPDLSSLSSPLIDAERNLGLLLGLHASYLAMSTPLSRIELECAKWLQSSIFFGGLQTSQIHYNYNEEKDEDHCSSPGTTTPDKAKLYCRRITLSDHAQPFLQAIADNTTQDHTVKDFLCQIERCCKQYHLITPITFPPEHPVEEVGRLLLCCLLKHQDLGHVALSLVDQCALGMDQGKQRSLPKSVIDVCRMVYQAKCSLIKTHQEQGRSYKEVCAPVIERLRFLFNELRPAVSNDLSIVSKLKLLSSQPRWKRITQKLIRDRRKKRVPKKSESADLEECKMEREGTNEEPPAPLSPAPVDKKQTTVKSAKQDKWQPLLNTIGSVQKYRWLRHSVQGSFSQSSLMSTIVEFSLKEEPLDVEKIRKCLLKQLERAEVRLEGIDTMLKLASKNFLLPSVQYAMLCGWQRVIPEGTNIGEPLADCLKDVDLIPPFNRMLLEVTFGKLYAWSIQNVRNILLEASVKFKELGVQPVPLQTITNENPAGPSLGTIPQARFLLAMLHMLTLKHGANSLSLLLNSGILALTQSVLRLIGPSCDSSEEELTSVAHGGSATVLEESRKETAPTPLPASGPELAAMMKISTRVMRGPDWKWGDQDGPSPGLGRVIGELGEDGWIRVQWDTGSTNSYRMGKEGKYDLKLAEPPPATQPATEDSDTEDDTEGEVMEKCAHPTAIMLTSTVNLLKTLSLSSGIHAEVMQVDAVSTLCGLLRILVESGANDKTGCSSHKLVSREQHRSWCTLGFIRSIALMPQMCSTLSSSAWINLLIRIVEGHQSFTAITLQRQILALRLLQAVLPSWDKTERSQDMKFLVEKLFGFLGSLLSTCSSDLPLLREGSMRRRKSRPQASLTATHSSTLAEEIVTVLRTLHSLSQWNALINEYINSQLSGIGDVMAGRHTEVALLEDYLPNAEAAAVGSLMAVLAVIGGIDGRLRLGGRVVHEEYGEGTVTRITPKGRITVQFHEMRNCKVCLLSQLKPLHMIPFSVQNLPFAEPMLMVWAQLVNLAGSKLEKQRMKKSLSRGLTADQVDVHLLRCQQLRLYILKAARALLSHQDKLRQILSQAAVVDVSPNPTEDQCASSPDVGDLSPEGPQPPLILLQQLLSASTQPSPIKAIFHRQELEAAALAVCQYLAVESTHPSSPLFEDSSSSEATTPVTVQHMRPPKQKKHKTSPVPPAPIVLQLMEMGFQRKNIEFALKSLSGTTGASGVPGVEALVGWLLDHPDVLITDLSDAETVSDEYSDEEVLEELEEPEPAFPVPPGVVVTESQTFKKRSDFQSNDDYAVYVRENIQVGMMVRCCRTYEEVYEGDVGKVIKLDRDGLHDLNVQCDWQQKGGTYWVRYIHTELLGFPPQSSPSHIKIGDKVRVKPSVTTPKYKWGSVTHRSVGVVKAFSANGKDVIVDFPQQSHWTGLLSEMELVPSVHPGVRCDGCQMFPINGPRFKCRSCDDFDFCESCFKTRKHNPRHSFGRINEPGQSPTFSGRSGKQLKRHHSSQRGMLIDEWSRVVKNLSVSSSVNQASRLIDCGEQCWQSSGSQGKHWIRLELFPDMLVHKLKMIVDPADSSYMPSLVVVSGGTSLNNLIELKTININPTDTVVSLLNDCTEYHRYIEVAIKQCRSSGIDCKIHGLSAVGRVRAEDEDLATVPFLASDNEEEEDEKTATGSMSRKRLSGLESAATIRTKVFVWGLNDKDQLGGLKGSKIKVPSFSETLSALNVVQVAGGSKSLFAVTAEGKVYACGEATNGRLGLGLSSGTIPIPRQISALSNYVVKKVAVHSGGRHAMALTVDGKVFSWGEGDDGKLGHFSRMNCDKPRLIEALKTKRIRDIACGSSHSAAITSSGELYSWGLGEYGRLGHGDNTTQLRPKLVKVLLGHRVVQVACGSRDAQTLALTDEGMVFSWGDGDFGKLGRGGSEGCNIPQNIERLNGQGVCQIECGAQFSLALTKSGVVWTWGKGDYFRLGHGTDVHVRKPQMVEGLRGKKIVHVAVGALHCLAVTETGQVFAWGDNDHGQQGNGTTTVNRKPTLVQGLEGQKITRVACGSSHSVAWTTVDVTTPSVHEPVLFQTARDSLGASYLGVPSDSDPSLLSNKLNGVNNVKPNRPSLAKILLSLDGNLAKQQALSHVLSSLQIMYARDAVVGALMPSCMMPLECPSSSPVPPSDCSSASSPCEAEGPALPSESEERVSPHPWMDSKRGEVTTSEDATTPSSAVTPSTASASSRPFIPVTDDPGAASIIAETMTKTKEDSENQSKAVGPEPQYLDEFTSLLIPDDTRVMVDLLKLAVSLRSGDKGKEVLSAVLSGMGTACPQVADMLLELCVTELEDVATDCQSGRLSSQPVVVESSHPYIDDTSTSGTVKIPGAEGLRVEFDRQCSTERRHDPLTIMDGANRIVSVRSGREWSDWSSELRIPGDELKWKFTSDGSVNGWGWRFTVYPIMPAAGPKDLLSDRCILSCPSMDLVTCLLDFRLNFASNRSIVPRLAASLAACAQLSALAAGHRMWALQRLRKLLTTEYGQSININRLLGDTEGEARSMSFTGSALAALVKGLPEALQRQYEYEEPIVRGGKQLLHSPFFKVLVALACDLELDTLPCCAETHKWAWFRRYCTASRVSVALDKRSPLPRPFLDEVAKKIRELMADHENMNTLHESHDLFKREQDEQLVQWMNRRPDDWTLSAGGSGTIYGWGHNHRGQLGGIEGAKVKVPTPTEALATLRPVQLIGGEQTLFAVTADGKLYATGYGAGGRLGIGGTESVSTPTLLESIQHVFIRKVAVNSGGKHCLALSSEGELYSWGEAEDGKLGHGNRSPCDRPRVIESLRGVEVVDIAAGGAHSACITASGELYTWGKGRYGRLGHGDSEDQLKPKLVEALQGHRVIDVACGSGDAQTLCLTDDDMVWSWGDGDYGKLGRGGSDGCKVPMKIDSLTGLGVVKVECGSQFSVALTKSGAVYTWGKGDYHRLGHGSDDHVRRPRQVQGLQGKKVIAIATGSLHCVCCTEDGEVYTWGDNDEGQLGDGTTNAIQRPRLVAALQGKKINRVACGSAHTLAWSTSKPTNAGKLPAQVPMEYNHLQEIAIIGLRNRLLLLHHISELFCPCIPMFDLEGRLGETGHGVSVGFDTLRGILISQGKEAAFRKVVQATMVRDRQHGPVVELNRIQVKRSRSKGGLAGPDGTKSVFGQMCAKMSSFSPDSLLLPHRVWKVKFVGESVDDCGGGYSESIAEMCEELQNGLTPLLIVTPNGRDESGANRDCFLLNPAAKSPLHISMFRFLGVLLGIAIRTGSPLSLYLAEPVWKQLAGMNLTIADLSEVDKDFIPGLMYIRDNEASAEEFEAMPLPFTVPSACGQEVQLSSKHSHISLENRSEYVRLAINYRLHEFDEQVSAVREGMARVVPVPLLSLFTGYELETMVCGSPDIPLHLLKSVATYKGVEPTAPLIQWFWEVMESFSNTERSLFLRFVWGRTRLPRTIADFRGRDFVVQVLDKYNPPDHFLPESYTCFFLLKLPRYSCKLVLEEKLKYAIHFCKSIDTDDYARIALSGEPAADDSSDDSDNEDADSFASDSTQDYLTGH

Target ORF information:

RefSeq Version	XM_009302643.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio HECT and RLD domain containing E3 ubiquitin protein ligase 2 (herc2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302643.2

ORF Insert Sequence:

ATGCCTTCTC CATCATTTTC CCTGAATGCC CAGCTGCGCT TCCATGATAA ATGGCTGAAG 
ATCGACTTGC AGCGCGCCTT CTCTCATGAA GGACTGAGCG AGATGTGGAA CGAGATGGTG 
AAGGATGAAG AGATCGTCTT TGACAGAGAA GAGTCTGCCC ATCCAGAGGA CTGTGCTGAT 
TGCACCCAAA AGAAAGATGA CACCAATGAT AAAGAAAAGA AGGAAGAGGA GGAGGCAGCA 
GCAACGGCGG CTTCATCTTT CCATCACTCC ATCATTGAAA TGTGGGACTG GGGGAGGCAG 
CCAGAAACCA GCGAGCTGCG GGACTGTCTG ATGGCTTTGG TAGACGACCA ACAGAAGCTG 
TCTTCTCAGA CGGCTAAGAG CACACTCTCA GCCCTGCGTC TAAGCCAGAG ACTGGTGATC 
CTGGAGCGCT ACCTCATCTC GCTCACTCAC AGCATGATGG AGGAGAAGTA CAAAGTACGG 
TGGAAGAGCC CTCCCAGTCC TCCACTCTCT GCTGTCGACA ACAAAAGCGC TCGGCCAGTT 
AGTAAAGGGG TGGAAGGTTT GGCCAGGGTG GGCTCAAGGG CAGCTCTTTC CTTTGCCTTT 
GCATTTCTCC GCCGAGCCTG GAGATCAGGA GATGATGCAG ACCTGTGCAG TGAGTTACTG 
CAGGAGTCTC TGGATGCTCT GCGGGCTCTT CCAGAAGCTA CTCTGTTTGA TGAGGGCACT 
GTATCTCCTG TTTGGCTGGA GGTGGTTGAG AGGGCTACTA AATTTCTAAG TGACATCCAT 
GGAAGTGCCA GTGGAAAAGC ACCCAGTAAC ATTCCCTTGC AGGATCAGCA CTTGGCCCTT 
GCCATCCTGC TGGAGCTCGC AGTGCAACGA GGAACTCTCA GTCAGCTGCT GTCTGCAGTT 
CTGCTGCTTC TGAGGCTCTG GGACAGTGGA ACTAGAGAGA TGGATAATGA GCGCTCCACC 
CAAGGCACCA GCGCCCCGTT ACTGCCCCTC CTGCAGCGCT TCCAGAACAT CCACAGCAGT 
AAAGAGGAGC CTGTTCCTGA AGAGGAGATT GAGATCCTTT CGGCCCCTCT GAGCCCGAAT 
GAGAGTTTTC TGCGCTACCT TACACTGCCT CAAGATAATG ACCTGGCCAT TGATTTGCGG 
CAGACAGCTG TAGTCATCAT GGCACATTTG GACCGCCTGG CAGCCCCATG CAGCCCCCCG 
CAGTGCAGCT CACCCACTTC TAACAAGGGG ACACTGCAGG AGGTGATTGG TTGGGGTCTG 
CTTGGATGGA AGCCTTATGC TAATGTGAAT GGACCAATTC AGTGCAAGGC ACTATCAAAT 
CTGGGTGTCA CGCAGATTGT CTGCTCAGAG AAGGGTTTCC TCATCCTGAC CATCACTGGT 
GCTGTCTACA CACAAAACTA CAAAAGTACT ACACTAGCAC CTATGCTAGT CCATGCACTG 
ACGTCCAGGA AAATAATGAA GCTTGCAGCT CATCCTGATG GCCAGCATTA TTTGGCTCTT 
TCAGTCAATG GAGAGGTTTT CTCCTGGGGT TGTGGAGATG GAGGCAGGCT AGGACATGGA 
GACACTACGT ATTTAGAGGA GCCCACTATG ATTTCAGTAT TCTCAGGGAG GCCGGTAGGG 
AAGCAGGTTG TACATATTGC CTGTGGCAGC ACATACAGCG CAGCCATCAC AGCAGATGGA 
GAGCTTCACA CATGGGGCCG AGGAAACTAC GGCAGACTGG GCCATGGCTC CAGTGAGGAC 
CAGACCACTC CCATGCTAGT AACTGCACTG AAGGGGCTGA AGGTGATCGA TGTGGCCTGT 
GGAAGCGGAG ATGCTCAGAC GCTTGCTGTG ACTGAGAATG GTCAGGTATG GTCATGGGGT 
GATGGAGATT ATGGGAAACT GGGCCGAGGG GGCAGTGATG GATGCAAAAC ACCAAAACTA 
GTGGAGAAGC TCCAGGATCT GGATATTGTG AAGGTGTGCT GTGGGAGCCA GTTCTCCGTA 
GCGCTCACTA AAGATGGACA AGTTTACACC TGGGGCAAAG GAGACAATCA ACGACTTGGA 
CATGGCACTG ATGAACATGT CCGCTATCCA AAACTACTTG ACAGTCTGCA AGGCAAGAGG 
GTGGTGGACA TAGCGGTTGG CTCCACACAC TGCCTGGCCC TTACAGAAGA AGGAGAGGTG 
CACAGCTGGG GCAGTAATGA CAAACTACAG CACTTTGACA CACTTTTCTC CAATAAGAAG 
CAGCCCAAAG CCTTGCCAGG CCTCAACTCC AAACACATAG TAGGCATCTC CTGCGGTCCT 
GGCCAGAGTT TTTCATGGTC ATCCTGCTCT GAGTGGTCTG TGGGTCTCCG GGTACCCTTC 
GTGGTAGACG TGTGCACTAT GACCTTTGAC CAGCTGGACC TGCTTCTGCG CCAGGTTAGT 
GAGGGCATGG ACGGCAGCTC TGATTGGCCA CCACCACAGG AGAAAGAATG CATGGTCGTG 
GCAGCTCTCA ACCTGCTCAG ACTACAGCTT CATGCAGCCA TCAGTAATCA GGTGGATCCC 
GAGGAGCTGG GTCTGGGTCT GGGCAGTGTG CTGCTTAATA ACCTCAAGCA GACGGTGGTG 
ACTCTGGCCA GCAACGGCGG GGTGCTAAAC ACTGTGCAGG CTGCCGCCCA GACCGTGCTC 
CAGAGTGGCT GGTCAGTGCT ACTCCCCACT GCAGAGGAAC GAGCAAGAGC CCTGTCCTCG 
CTGCTGCCCA ATGCAGCATC TGCTAATGAG ATGAGTGTAA GTCCTGGCCG TCGCTTCATG 
ATCGACCTCT TGGTTAGTAG TCTGATGGCA GATGGAGGGT TGGAGTCTGC TTTGAATGCT 
GCCATCACGG CCGAGATCCA GGATATTGAG GCAAAGAAAG AGGCTCAGAA AGAGAAGGAA 
ATCGATGAGC AGGAAGCCAG CGCCTCTTCT TTGCATCGCT GTCGCACCAC ACTGGACAAA 
GACCTGATCA ACACCGGCAT CTATGAGTCT GCCAACAAAC AGAGCCTCCC ATTGGTCCAG 
CTTGTCCAAC AGCTGCTCAG GAACATTGCT TCACAGACTA TAGCCCGTCT TAAGGATGTG 
GCCCGACGCA TCTCAAATTG TCAAGACACC GAGCAGGTCA GCAAGGAGCG CTCAGCCTCT 
CTGGACCTGC TGCTGCGCTT TCAGAGGCTT TTGGTCTGCA AATTGTATGT CGGAGTCAAT 
GGCACTGTGG GTGTGGGAGG ATACACTTTG AGTCACTCAG AAATTCTGGG AGTTGGCTCT 
CTCCTGAAGA AGTATATGGC TTTGCTTTGC ACACACATCG GTGATATCCT TCCTGTGGCC 
ACCAGCATTG CCCTCAACAG CCATCGTCAC TTTGCAGAGG TGGCTCGTGT CCTTGAGGGA 
GATCTTACAG GTGTTCTGCT ACCAGAGCTC ATTGTGTCCA TTGTGCTGCT GCTGACCCAT 
GATGCTGGAC TAATGCAAGA GACTGGATCC ATTCCTCTGC TCGCTGCTCT TCTGGAGCAC 
TTGGACCGTT TTAACTATCT TGCACCTGGT ACAGAAAGAG ATGACAATGA GGATCTTGCC 
TGGCCTGGAC TCATGGGTTT CTTTTTCAAT GGACCGAGCT CAAAGAACAA CGAGGAGGTG 
TCACTTATCC GCAAAGCAGA CCTGGAGAAT CACAACAAAG ATGGCGGCTT TTGGACTGTA 
ATTGAAGGGA AGGTCTATGA CATAAAGGAC TTTCAAGCTC AGACTCTAAC TGGCAACAGC 
ATCCTTGCCC AGTTTGCAGG AGAAGACCCT GTGGTGGCAT TAGAGGCTGC CCTCCAATTT 
GAAGACACCA GAGAGTCCAT GCAAGCTTTC TGTGTGGGAC AGTATATGGA GCCCAACCAA 
GAAATGGTGA CAACGCCTGA CCTGAGCAGC CTGTCCTCTC CTCTTATAGA TGCTGAGAGG 
AACCTTGGTC TGCTTCTGGG GCTACATGCC TCATATCTGG CTATGAGCAC CCCTCTCTCC 
CGCATAGAGT TAGAATGTGC CAAATGGCTC CAGTCATCTA TTTTCTTCGG TGGACTTCAG 
ACCAGTCAGA TCCACTATAA TTATAACGAG GAGAAGGATG AGGACCACTG CAGCTCTCCA 
GGGACCACCA CACCAGACAA AGCCAAACTG TACTGCCGCA GAATCACCCT GAGTGATCAC 
GCCCAGCCCT TCCTGCAGGC CATCGCTGAC AACACCACCC AGGATCACAC TGTGAAGGAC 
TTTTTATGCC AAATCGAGCG GTGCTGTAAA CAGTACCATC TCATCACTCC CATCACTTTT 
CCACCTGAAC ATCCTGTGGA GGAAGTGGGT CGCCTGCTGC TCTGCTGCCT TCTGAAGCAT 
CAGGATCTTG GTCATGTCGC CCTCTCCCTA GTTGATCAAT GTGCCTTGGG TATGGACCAG 
GGGAAACAGA GGTCCCTCCC GAAATCTGTG ATTGATGTGT GTCGAATGGT GTATCAGGCC 
AAGTGTTCGT TGATAAAAAC ACACCAGGAA CAGGGTCGTT CTTATAAAGA GGTGTGCGCT 
CCTGTTATTG AGAGGTTGCG TTTCCTGTTT AATGAGCTAC GACCTGCCGT CAGTAATGAC 
TTGTCCATTG TGTCCAAACT CAAACTGCTG AGCTCTCAGC CTCGCTGGAA GAGGATCACA 
CAGAAACTGA TCAGAGACCG CCGCAAAAAG AGAGTGCCTA AAAAGTCGGA GTCAGCTGAT 
CTGGAGGAGT GCAAGATGGA GAGGGAAGGG ACCAATGAGG AACCACCTGC TCCTCTAAGC 
CCCGCTCCTG TTGACAAGAA GCAAACAACT GTCAAATCTG CAAAGCAGGA TAAGTGGCAG 
CCTCTGCTAA ACACCATCGG CAGTGTTCAG AAGTACCGCT GGTTAAGGCA CAGCGTTCAG 
GGCTCCTTCT CTCAGTCCAG CCTCATGAGC ACCATCGTTG AGTTTTCTTT GAAAGAGGAG 
CCGCTGGATG TGGAGAAGAT CAGAAAGTGC CTCCTTAAAC AGCTGGAGAG AGCTGAGGTC 
CGCCTGGAGG GTATTGACAC TATGTTGAAG CTGGCATCTA AAAACTTCCT CCTGCCCTCT 
GTTCAGTATG CCATGCTTTG TGGCTGGCAG AGAGTCATAC CTGAAGGGAC CAATATTGGA 
GAGCCATTGG CTGACTGTCT AAAGGATGTA GATCTCATTC CTCCATTCAA CCGCATGCTA 
CTAGAGGTGA CGTTTGGGAA GCTCTATGCC TGGTCCATAC AGAACGTCCG CAACATCTTG 
TTGGAGGCTA GTGTCAAGTT CAAGGAACTG GGTGTGCAAC CAGTGCCTCT CCAGACAATC 
ACTAATGAGA ACCCAGCAGG CCCCAGTCTG GGTACCATCC CTCAGGCACG CTTCCTGCTG 
GCTATGCTCC ATATGCTCAC TCTCAAACAC GGAGCCAACA GCCTCAGCCT CCTGCTGAAC 
TCTGGCATCC TCGCTCTCAC CCAGAGTGTC CTCCGACTCA TTGGTCCTAG CTGTGATAGC 
AGTGAGGAGG AGCTGACCTC TGTAGCTCAT GGAGGCTCTG CCACAGTTCT GGAAGAGTCC 
AGAAAGGAGA CTGCCCCGAC CCCTCTGCCC GCTTCCGGCC CTGAGCTGGC AGCTATGATG 
AAGATCAGCA CAAGGGTGAT GAGAGGTCCT GACTGGAAGT GGGGGGACCA GGATGGCCCA 
TCACCAGGCC TGGGTCGTGT GATTGGGGAG TTGGGAGAAG ATGGTTGGAT AAGAGTCCAA 
TGGGACACCG GCAGCACCAA CTCCTACAGG ATGGGAAAAG AGGGCAAGTA TGACCTTAAA 
CTGGCTGAGC CGCCACCTGC CACCCAACCA GCCACTGAAG ACTCTGACAC GGAGGATGAC 
ACAGAAGGGG AGGTGATGGA AAAGTGTGCC CACCCCACGG CAATCATGCT GACTAGTACA 
GTAAATCTGT TGAAGACACT GTCACTGTCT TCTGGGATTC ATGCAGAGGT GATGCAGGTC 
GATGCGGTCA GTACTCTGTG CGGGCTACTC AGGATACTGG TGGAGAGTGG AGCCAACGAC 
AAGACTGGTT GCTCCTCTCA CAAGCTGGTG TCCCGAGAGC AGCACAGGAG CTGGTGCACG 
CTGGGATTTA TTCGCAGTAT AGCTCTTATG CCTCAGATGT GCAGTACCCT GAGCTCCTCT 
GCCTGGATCA ATCTGCTTAT ACGCATCGTG GAGGGTCATC AGTCCTTCAC TGCAATCACA 
CTACAGAGAC AGATTCTTGC CCTGCGGCTT CTACAGGCAG TATTGCCCTC CTGGGATAAG 
ACTGAACGCT CTCAGGATAT GAAGTTCTTG GTGGAGAAAC TGTTTGGGTT CCTCGGCAGC 
TTGCTCAGCA CTTGTTCTTC AGATCTGCCT CTTCTTAGGG AAGGTTCAAT GCGTAGGAGG 
AAGTCCCGCC CCCAGGCCTC ACTGACAGCC ACTCATAGCA GTACTTTGGC AGAGGAAATA 
GTGACAGTAT TGCGCACGCT TCACTCCCTC AGCCAATGGA ATGCCCTGAT TAATGAATAC 
ATTAACTCTC AGCTCAGTGG AATCGGGGAT GTCATGGCAG GCCGACACAC TGAAGTGGCT 
TTGCTTGAAG ACTATCTCCC CAATGCGGAA GCAGCAGCGG TGGGCAGTCT GATGGCTGTT 
TTGGCAGTCA TCGGGGGGAT TGATGGACGT CTGCGTCTGG GTGGACGTGT GGTCCATGAG 
GAGTATGGAG AAGGCACAGT GACTCGCATC ACACCTAAAG GACGAATCAC TGTTCAGTTT 
CATGAGATGA GGAACTGCAA AGTGTGCCTG CTCAGTCAGC TCAAACCGCT TCACATGATT 
CCATTCAGCG TTCAGAATCT GCCCTTCGCT GAGCCCATGC TAATGGTGTG GGCCCAGCTA 
GTCAACTTGG CTGGGAGCAA GCTGGAGAAA CAACGCATGA AGAAATCTCT TAGTAGAGGA 
CTTACAGCGG ATCAGGTAGA TGTACATTTG TTGCGCTGTC AGCAGCTCAG GCTGTACATC 
TTGAAGGCTG CACGAGCTCT ACTCTCCCAT CAGGACAAAC TCAGACAGAT CCTTTCTCAG 
GCGGCCGTGG TTGATGTCAG CCCCAATCCT ACAGAGGATC AGTGCGCGTC GTCTCCTGAT 
GTAGGAGATT TGTCTCCTGA GGGTCCTCAG CCTCCTCTCA TCCTCCTTCA GCAGTTGCTG 
TCAGCATCCA CTCAACCTTC ACCCATCAAA GCCATATTCC ACCGCCAGGA GTTAGAGGCA 
GCGGCTCTGG CTGTGTGTCA GTACCTGGCG GTTGAATCCA CACACCCGTC ATCGCCTCTG 
TTTGAGGACA GCAGCTCTAG CGAAGCCACT ACACCAGTCA CTGTGCAGCA CATGCGTCCA 
CCCAAACAAA AGAAACACAA GACGTCACCT GTGCCCCCTG CTCCCATCGT CCTGCAGCTG 
ATGGAGATGG GCTTTCAAAG GAAAAACATC GAGTTTGCTC TTAAATCACT GTCTGGAACC 
ACAGGTGCTT CTGGAGTGCC AGGTGTCGAG GCATTGGTGG GCTGGTTGTT GGACCATCCA 
GATGTTCTCA TTACAGATCT GTCGGATGCA GAGACTGTAT CAGATGAATA CTCTGATGAA 
GAGGTGCTGG AAGAGCTTGA GGAGCCTGAG CCTGCCTTTC CTGTGCCTCC TGGGGTTGTG 
GTGACTGAGA GTCAGACGTT TAAAAAGAGG TCGGACTTTC AGAGCAATGA CGATTATGCT 
GTTTACGTGA GAGAGAACAT TCAGGTGGGC ATGATGGTAC GGTGTTGCAG GACATATGAG 
GAAGTGTATG AAGGAGATGT GGGGAAAGTG ATCAAGCTTG ACAGAGACGG CCTGCATGAC 
CTGAATGTTC AGTGTGACTG GCAGCAGAAA GGAGGAACTT ACTGGGTCCG TTACATCCAC 
ACTGAGCTGC TGGGGTTTCC CCCTCAGAGT TCTCCATCTC ATATAAAGAT TGGTGATAAA 
GTGCGAGTGA AGCCCTCGGT CACAACACCA AAATACAAGT GGGGGTCAGT CACTCATAGG 
AGTGTGGGTG TGGTCAAAGC CTTCAGTGCC AATGGGAAGG ATGTCATTGT GGACTTTCCT 
CAGCAGTCGC ACTGGACTGG ACTGCTGTCA GAGATGGAGC TTGTGCCCAG CGTACATCCT 
GGTGTCAGGT GTGACGGATG TCAGATGTTC CCTATAAATG GCCCAAGGTT TAAGTGTCGG 
AGCTGTGATG ACTTTGACTT TTGTGAGAGC TGCTTCAAGA CCCGTAAACA CAATCCTAGG 
CATTCATTTG GGAGGATAAA CGAACCAGGT CAGTCACCCA CCTTTAGTGG GCGCTCAGGA 
AAGCAGCTGA AAAGACATCA CAGCAGTCAG CGTGGCATGC TGATCGACGA GTGGTCAAGA 
GTGGTGAAGA ACCTCAGCGT ATCGTCTTCT GTTAACCAGG CGTCTCGACT CATCGACTGT 
GGAGAGCAAT GCTGGCAGTC CTCAGGCTCT CAGGGCAAGC ACTGGATCAG ATTGGAGCTT 
TTCCCAGACA TGTTAGTCCA CAAACTGAAA ATGATTGTAG ATCCAGCTGA CAGCAGCTAC 
ATGCCTTCAT TGGTGGTGGT ATCAGGTGGA ACCTCACTCA ACAATTTGAT CGAGTTGAAG 
ACCATCAACA TCAATCCCAC TGACACTGTC GTCTCTCTAC TCAATGACTG CACAGAGTAT 
CACAGGTACA TTGAGGTTGC TATAAAGCAG TGTCGAAGTT CAGGTATTGA CTGTAAGATT 
CATGGCTTGA GTGCTGTGGG ACGTGTCCGT GCCGAGGATG AAGATCTTGC AACTGTACCT 
TTCCTGGCAT CTGACAACGA GGAAGAGGAA GATGAGAAGA CCGCGACTGG AAGTATGTCT 
CGAAAGAGAT TGTCAGGCCT CGAGTCTGCA GCCACTATCA GAACCAAAGT CTTTGTCTGG 
GGTCTTAATG ATAAGGACCA ACTTGGAGGA CTTAAGGGGT CCAAGATCAA AGTCCCTTCA 
TTCTCAGAGA CTCTGTCTGC TCTGAATGTA GTGCAGGTGG CTGGTGGCTC TAAAAGTCTT 
TTTGCTGTGA CTGCTGAAGG TAAAGTATAT GCCTGTGGAG AGGCCACCAA TGGAAGACTT 
GGTTTAGGCC TCTCCAGTGG AACCATCCCA ATTCCCAGAC AGATATCAGC TCTCAGCAAC 
TATGTGGTCA AGAAGGTTGC TGTGCATTCA GGTGGACGGC ATGCCATGGC ACTTACCGTG 
GATGGGAAAG TATTCTCTTG GGGTGAAGGA GACGATGGCA AGCTTGGACA CTTCAGCCGA 
ATGAACTGTG ATAAGCCAAG GCTGATCGAG GCATTGAAAA CCAAACGCAT TCGAGATATT 
GCGTGTGGCA GCTCTCACAG TGCCGCCATC ACCTCCAGTG GAGAGCTGTA CAGCTGGGGT 
CTGGGGGAAT ATGGCCGTCT GGGCCATGGG GACAACACCA CCCAACTCAG ACCCAAACTG 
GTCAAGGTGC TTTTAGGCCA TCGCGTTGTC CAGGTAGCTT GTGGAAGTAG AGATGCCCAA 
ACTCTTGCAC TGACAGATGA AGGAATGGTG TTCTCATGGG GAGATGGAGA CTTTGGGAAA 
CTGGGCCGTG GTGGCAGCGA GGGATGTAAC ATTCCTCAAA ACATTGAGCG TCTGAATGGA 
CAGGGGGTCT GTCAAATTGA ATGTGGAGCT CAATTCTCCC TCGCCCTCAC CAAATCTGGA 
GTTGTCTGGA CCTGGGGTAA AGGGGATTAC TTCAGACTGG GTCACGGCAC AGATGTTCAT 
GTCCGCAAAC CCCAGATGGT TGAAGGACTT AGGGGGAAGA AGATTGTTCA TGTGGCCGTT 
GGTGCTCTGC ACTGCCTGGC TGTCACCGAG ACTGGACAGG TGTTCGCGTG GGGGGATAAT 
GACCATGGGC AGCAGGGGAA TGGCACAACC ACAGTCAACA GGAAGCCCAC ATTGGTGCAG 
GGTCTAGAGG GTCAGAAAAT TACTCGTGTG GCCTGCGGTT CTTCTCATAG TGTTGCCTGG 
ACCACAGTAG ATGTCACTAC ACCCTCCGTC CATGAGCCTG TGCTCTTCCA GACTGCTAGA 
GACTCTCTGG GAGCCTCTTA CCTTGGTGTG CCATCAGATA GTGACCCGTC TTTACTGAGC 
AATAAGCTAA ATGGCGTAAA CAATGTGAAG CCCAACAGGC CATCTCTGGC AAAGATCCTT 
CTCTCTCTGG ATGGTAATCT GGCCAAACAG CAGGCCCTAT CCCATGTGCT GTCTTCCCTG 
CAGATCATGT ATGCCAGAGA TGCAGTGGTG GGTGCATTAA TGCCATCCTG TATGATGCCT 
CTGGAGTGTC CCTCCAGCTC TCCGGTGCCC CCATCAGACT GTTCATCAGC TTCCAGCCCC 
TGTGAGGCTG AGGGTCCTGC CCTGCCCTCA GAGAGTGAGG AGAGGGTCAG TCCGCACCCC 
TGGATGGACA GCAAACGAGG AGAGGTAACC ACGTCTGAGG ATGCCACTAC CCCATCTTCA 
GCAGTGACCC CTTCTACTGC CTCTGCCTCA TCTCGACCCT TCATACCAGT CACAGATGAT 
CCTGGTGCTG CCAGCATCAT TGCTGAGACC ATGACCAAAA CCAAAGAGGA CTCTGAGAAC 
CAGAGTAAAG CCGTGGGTCC AGAGCCACAG TATCTGGATG AGTTCACCAG TCTCCTGATC 
CCTGATGACA CGCGTGTGAT GGTGGACCTG TTAAAACTAG CTGTATCTCT GCGCTCTGGT 
GATAAAGGAA AGGAAGTGCT GTCTGCTGTT CTGTCTGGCA TGGGTACAGC CTGCCCACAG 
GTGGCGGATA TGCTTCTGGA GCTGTGTGTG ACTGAGTTGG AGGACGTGGC GACTGACTGT 
CAGAGTGGAC GGCTTTCCTC TCAACCTGTG GTGGTGGAAA GCAGCCATCC ATATATTGAC 
GATACATCCA CCAGCGGAAC TGTCAAAATA CCAGGTGCTG AGGGTTTGCG GGTTGAGTTT 
GATCGCCAGT GCTCAACAGA AAGGAGACAT GACCCTCTTA CCATCATGGA TGGAGCCAAT 
AGAATTGTGT CTGTTCGATC AGGTCGTGAG TGGTCTGATT GGTCCAGTGA ATTGAGAATT 
CCAGGAGACG AGCTCAAATG GAAGTTCACC AGTGACGGCT CTGTGAACGG CTGGGGCTGG 
CGCTTCACAG TCTATCCCAT CATGCCTGCG GCTGGTCCTA AAGATCTTCT GTCAGACCGC 
TGCATTTTGT CTTGCCCTTC AATGGATCTA GTAACATGTC TGCTAGACTT TCGTCTGAAC 
TTTGCCTCAA ACCGCAGCAT TGTGCCCAGA TTAGCAGCCT CACTTGCTGC TTGTGCGCAG 
CTCAGCGCCT TGGCGGCTGG CCATCGTATG TGGGCTCTGC AGAGGTTACG CAAGCTCTTA 
ACTACTGAAT ACGGCCAATC CATTAACATC AACCGCCTCT TAGGAGACAC AGAAGGAGAG 
GCTCGCTCTA TGAGTTTTAC AGGAAGTGCA TTAGCTGCTC TGGTGAAAGG ACTTCCTGAG 
GCATTACAGA GACAGTATGA GTACGAGGAG CCCATAGTTA GGGGTGGCAA ACAGTTACTC 
CACAGCCCGT TCTTCAAGGT GTTGGTGGCG CTGGCGTGTG ATCTGGAGTT GGACACTCTT 
CCATGTTGTG CTGAGACACA CAAGTGGGCG TGGTTTCGAC GCTATTGCAC CGCCTCCAGA 
GTGTCAGTGG CTCTGGACAA GAGGAGCCCT TTGCCCCGCC CTTTTCTGGA TGAGGTGGCA 
AAGAAAATCC GTGAGCTGAT GGCAGATCAT GAGAACATGA ACACGCTTCA TGAGAGCCAT 
GACTTGTTTA AGAGGGAGCA GGATGAGCAG TTGGTTCAGT GGATGAACAG ACGACCAGAT 
GATTGGACAC TCTCTGCGGG TGGCAGTGGT ACAATATACG GCTGGGGGCA CAATCACCGG 
GGTCAGTTAG GTGGCATTGA AGGAGCCAAA GTAAAGGTTC CCACACCAAC AGAAGCTCTA 
GCCACACTGC GGCCAGTTCA GCTTATTGGA GGAGAGCAAA CACTGTTTGC CGTCACTGCT 
GATGGGAAGC TGTATGCCAC AGGATATGGT GCCGGTGGTC GACTGGGAAT AGGAGGGACA 
GAGTCCGTCT CCACTCCGAC CCTGCTGGAG TCCATCCAGC ATGTTTTCAT CAGAAAGGTG 
GCTGTGAACT CTGGAGGGAA ACACTGTTTG GCTCTTTCCT CTGAAGGAGA GCTGTACTCC 
TGGGGGGAGG CCGAAGATGG AAAACTGGGC CATGGAAACA GAAGCCCATG TGATCGTCCA 
CGCGTGATTG AGTCTCTCAG AGGGGTGGAA GTGGTGGACA TTGCTGCCGG TGGGGCTCAC 
AGTGCCTGCA TTACTGCCAG TGGAGAGCTC TACACATGGG GCAAAGGTCG CTATGGGCGC 
CTGGGTCATG GAGACAGCGA AGACCAACTT AAACCAAAAC TGGTGGAGGC TCTTCAGGGT 
CACCGGGTGA TAGATGTGGC ATGTGGCAGT GGAGATGCAC AGACCCTCTG TCTGACTGAT 
GATGACATGG TATGGTCGTG GGGCGATGGA GACTATGGCA AATTGGGCCG AGGAGGTAGT 
GATGGATGTA AAGTGCCGAT GAAGATCGAT TCATTAACCG GATTGGGTGT TGTGAAAGTG 
GAATGTGGCT CTCAGTTTTC TGTGGCCCTT ACCAAGTCTG GAGCAGTTTA CACATGGGGG 
AAGGGTGACT ATCACAGACT GGGACACGGC TCAGATGATC ATGTTCGCAG ACCCCGGCAG 
GTCCAGGGTT TACAAGGCAA AAAGGTCATC GCAATAGCAA CTGGTTCCCT TCACTGCGTT 
TGCTGCACGG AGGACGGGGA AGTGTACACA TGGGGTGACA ATGATGAGGG TCAGCTTGGG 
GACGGTACGA CCAATGCTAT TCAGAGACCC AGGCTGGTGG CAGCGCTGCA GGGCAAGAAG 
ATTAATCGTG TGGCCTGTGG TTCAGCACAT ACACTTGCCT GGTCCACTAG TAAACCAACC 
AATGCTGGAA AACTCCCTGC TCAGGTTCCA ATGGAGTACA ACCACCTGCA AGAAATCGCC 
ATCATCGGCC TGAGGAATCG GTTACTGCTA CTGCACCACA TCTCTGAGCT CTTCTGCCCC 
TGCATCCCAA TGTTTGACTT AGAAGGACGC CTGGGTGAAA CTGGACATGG TGTGTCTGTT 
GGCTTTGACA CGCTCCGTGG AATCCTCATA TCACAGGGCA AGGAGGCAGC ATTCCGCAAA 
GTGGTGCAGG CTACCATGGT CAGAGACAGG CAGCATGGGC CAGTGGTTGA GCTGAACAGG 
ATACAGGTCA AGCGTTCACG TAGTAAAGGA GGTCTGGCAG GGCCGGATGG CACCAAGTCA 
GTATTTGGGC AGATGTGTGC TAAGATGAGT TCTTTCAGTC CAGACAGTCT GCTGCTGCCA 
CACCGCGTCT GGAAGGTCAA GTTTGTGGGT GAGTCGGTGG ATGACTGTGG TGGCGGATAC 
AGTGAATCTA TCGCAGAAAT GTGCGAGGAG CTTCAGAATG GCCTCACTCC TCTGCTCATC 
GTCACTCCTA ATGGCAGAGA CGAGTCAGGA GCGAACAGAG ATTGCTTTCT GCTCAACCCA 
GCTGCCAAAT CCCCTCTGCA CATCAGCATG TTCCGCTTCC TAGGGGTGCT CCTGGGCATA 
GCGATCCGAA CAGGAAGTCC TCTTAGTCTG TATCTGGCCG AACCTGTGTG GAAACAGCTG 
GCAGGCATGA ACCTGACCAT TGCTGATCTC AGTGAGGTGG ACAAGGACTT CATCCCCGGT 
CTGATGTATA TTAGAGATAA CGAGGCGTCT GCGGAGGAGT TTGAGGCGAT GCCTCTTCCC 
TTCACTGTGC CCAGTGCCTG CGGACAAGAA GTCCAGCTCA GCTCCAAACA TTCCCACATC 
AGCCTTGAAA ACCGCAGTGA ATATGTGCGG CTAGCAATAA ATTACAGGCT GCATGAGTTT 
GATGAGCAGG TGTCTGCTGT AAGAGAAGGT ATGGCGCGTG TGGTTCCGGT ACCGCTCCTC 
TCTCTATTTA CCGGCTATGA GCTGGAGACC ATGGTGTGCG GAAGTCCGGA CATCCCTCTG 
CATCTGCTCA AATCAGTGGC CACATATAAA GGAGTAGAGC CCACTGCACC GCTCATCCAG 
TGGTTCTGGG AGGTGATGGA GTCTTTCTCT AACACAGAAC GCTCGCTCTT CCTCAGGTTC 
GTCTGGGGCC GCACGCGTCT TCCCAGGACC ATCGCAGACT TCCGTGGACG AGACTTTGTA 
GTGCAGGTGC TGGATAAATA TAACCCTCCC GACCACTTCC TCCCCGAGTC CTACACCTGC 
TTCTTCCTGC TCAAGCTGCC TCGCTACTCC TGCAAGCTAG TCTTGGAGGA GAAACTCAAA 
TACGCCATTC ACTTCTGCAA GTCCATCGAC ACTGATGACT ACGCACGGAT CGCTCTGAGC 
GGAGAACCGG CCGCAGACGA CAGCAGTGAT GACTCGGACA ATGAAGACGC AGACTCATTC 
GCTTCTGACT CCACACAGGA TTACCTGACA GGCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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HERC2 cDNA ORF clone, Danio rerio(Zebrafish)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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