hcn4 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	hcn4
Entrez Gene ID	557843
Full Name	hyperpolarization activated cyclic nucleotide-gated potassium channel 4
Synonyms	si:ch211-203b17.1
General protein information	Preferred Names hyperpolarization activated cyclic nucleotide-gated potassium channel 4 Names potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_680986.6	XP_686078.4	potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3
XM_680986.7	XP_686078.4	potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	HCN4	XP_002804905.1
Mus musculus (house mouse)	Hcn4	NP_001074661.1
Danio rerio (zebrafish)	hcn4	XP_686078.4
Xenopus tropicalis (tropical clawed frog)	hcn4	XP_004916024.1
Gallus gallus (chicken)	HCN4	XP_425050.3
Pan troglodytes (chimpanzee)	HCN4	XP_003952750.1
Bos taurus (cattle)	HCN4	XP_002690539.1
Homo sapiens (human)	HCN4	NP_005468.1
Danio rerio (zebrafish)	hcn4l	XP_684730.5
Rattus norvegicus (Norway rat)	Hcn4	NP_067690.1

Gene Ontology

The following hcn4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hcn4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa23006	XM_680986.7 Latest version!	Danio rerio hyperpolarization activated cyclic nucleotide-gated potassium channel 4 (hcn4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
ODa23006	XM_680986.6	Danio rerio hyperpolarization activated cyclic nucleotide-gated potassium channel 4 (hcn4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hcn4 ( XM_680986.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_686078.4 Danio rerio hyperpolarization activated cyclic nucleotide-gated potassium channel 4 (hcn4), mRNA.

Price & Availability↑

CloneID	ODa23006
Clone ID Related Accession (Same CDS sequence)	XM_680986.6 , XM_680986.7
Accession Version	XM_680986.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_680986.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGGT TGCATTCGTC CATGCGTAAA AGGCTCTACA GTTTGCCACA GCACATCGGG 
CAGAAAGCGT CCATCATGGG TGATGCTGAA GACTCAGACA AGGACACCCG GAGGAAAAGC 
ATCCGGATGA AGCCTTTACC GACCAGCGGG GAGATTAAAA GCGGAGAATC CGCGATCATG 
GAGACGGACA TCGGGAGACC CGTGAAGACC AGCTCCAACG GGGACTGCCG GAGGTTCCGC 
GGCAGCCTTT CGTCCATCAC CAGTCGGCAC GTCCACGACT CGGATGTAGC CGAGGAAAGG 
CGTCTGATCA CGGACGGAGA GGTCACTCCT AGCGAGGAGA GCCCTCCTGG AGCCGCGGGT 
GGCGGCGCGG AGCCCGGTGC GCAGAGCGGC GGGGGAGGCG GAGAGGAAGC CGCCGGTTCG 
AGCCAGCATC AGGGCATCCC GGATCAGCAG GCTGGCTTTA TTAAGCTGGA AGGCATCGAG 
CAGATACTGC CGGACGACGA GAGGTATTAT CAAGCGGGCT TCATGCACAG GCAGTTCGGG 
GCGATGCTGC AGCCGGGGGT CAACAAGTTT TCCCTGAGGA TGTTTGGGAG CGAGAAGGCG 
GTGGAGCGGG AGCAGGAACG GGTCAAATCC GCCGGCTTCT GGATTATTCA CCCCTACAGC 
GATTTCAGGT TTTACTGGGA TCTGACTATG CTGCTCCTGA TGGTGGGCAA TCTAATCATC 
ATCCCGGTCG GCATCACCTT CTTTAAAGAT GAGCACACGC CGCCGTGGAT CGTCTTTAAC 
GTGGTCTCCG ACACTTTCTT CCTGATGGAT CTGGTGCTGA ACTTCCGCAC GGGGATCGTA 
AAGGAGGACA ACGCAGAAAT CATCCTGGAC CCTCAGCAGA TCAAGATCAA GTATCTGCGC 
TCGTGGTTCG TGGTGGACTT CATCTCCTCC ATTCCCGTGG ACTATATCTT CCTCATAGTG 
GAGACGCGCA TCGATTCGGA TTTCTACAAG ACGGCGCGAG CTCTGCGGAT CGTCAGATTC 
ACTAAAATCC TCAGTCTGCT GCGGCTGCTG CGGCTCTCGC GCCTCATACG ATACATCCAC 
CAGTGGGAGG AGATTTTTCA TATGACCTAT GACCTGGCCA GTGCAATGGT GCGAATCGTC 
AACTTGATAG GGATGATGCT GCTGCTCTGC CACTGGGACG GATGTCTGCA GTTTCTGGTG 
CCCATGCTGC AAGACTTCCC TGCTGACTGC TGGGTGGCCA AAAACAAGAT GGTGAACGAC 
ACGTGGGGTC AGCAGTATTC CTACGCTCTC TTCAAGGCCA TGAGCCACAT GCTGTGTATA 
GGATATGGCA TGTATCCTCC CGTGGGCATG ACAGACGTGT GGTTGACCAT CCTCAGCATG 
ATCGTGGGTG CCACGTGTTA CGCCATGTTT GTTGGGCACG CCACAGCGCT GATCCAGTCC 
CTGGACTCCT CGCGCAGGCA GTATCAGGAG AAGTATAAGC AGGTGGAGCA GTACATGTCC 
TTCCATAAGC TGCCAGCAGA CATGAGACAG CGGATCCACG ATTACTACGA GCACAGATAC 
CAGGGCAAGA TGTTTGATGA AGAGAGCATT CTGGGAGAAC TCAATGAGCC ACTGAGAGAG 
GAGATCATAA ACTTCAACTG TCGGAAGCTG GTAGCGTCGA TGCCACTGTT CGCCAATGCA 
GATCCTAATT TTGTGACATC GATGCTCACT AAACTTCGCT TCGAGGTCTT CCAGCCGGGC 
GACTACATCA TACGAGAAGG AACCATCGGG AAGAAGATGT ACTTCATCCA GCACGGTGTG 
GTGAGCGTCC TTACCAAAGG AAACAAGGAG ACCAAGCTGT CGGACGGATC CTACTTTGGA 
GAGATCTGTC TGCTGACGCG GGGTAGACGC ACAGCCAGTG TCAGGGCCGA CACATACTGC 
CGTCTGTACT CTCTGTCAGT GGACAACTTC AACGAGGTGC TGGAGGAGTA TCCCATGATG 
AGGAGAGCGT TTGAGACTGT TGCCCTCGAC CGGCTCGACC GCATCGGAAA GAAGAACTCA 
ATTCTCCAGC ACAAAGTCCA GCACGACTTG AATTCAGGAG TGCTGAACTA CCAGGAAAGT 
GAGATCATCC AGCAGATCGT GCAGCATGAT CGGGACATGG CCCACTGTGC AAACCTGCTG 
CAGAGTCCGT CTCCGCCTGC CCCTCCGTCC CCGACTCCGG TCATCTGGGC TCCACTGATC 
CAGGCTCCTC TACAAGCCGC TGCAGCCACC ACATCGGTCG CTATTGCACT AACACACCAC 
CCACATCTAC CCGCCACGCT CTTCCGACCT CCCGTTTCCC TGCTCGGATC CAGAAACGAG 
CCTCCCAGCA GACTCAAGAG ATTTCAGACG GTTGCACCAA GAACAGGATC AACCACTGAC 
TCTCCATCCA CCTCCCCATC AAAGCTTCAT TCAGGAGTGG ATACTCCTCT TCTAGCATCC 
CTTCATACTC AACACTCATC AGTCACCATC ACTCCCAGTG CTACCACCAA TCAACCAGTG 
TCATTCAGAA GCTTCAGTTC TCCATCAGCA TCTCCGACGT TAAGCACGGC GCAGCTTCAT 
CCACAACCGA GACAAAAACA GCCGAGCACG CCCCCTCTAT CAGCACGCCT GCAAGCAGCA 
GGAGCACATC CGCCCGGCAT CCTCACTACA GCCTCATCAA ACACTAGTGC ACTTTCAGCA 
GGACTATCCA CTCATTCCCC TACTCACAGT TATCCTCCAC CAAAACACCC TCAAACGGGC 
TCCCTACAGT TCGCATCCGG AGGAGGGAAG AGTGGGTTAA CCCTGTTTCA CAGTCCGCCA 
CCCGGATCTC CAATCTCCAT CCATTCCCAA GCCCCCGAGA GCTCCACGTC ATCCCAGACG 
CAGCCCTCTT TTTCCAGTTA CCTTGCTTTG GAAAGGTCAG CGCTGGCCTC GATCGCTCAG 
TACGGCTCAG CGAACGCCTC GCCGAGCTAC ACGCCTTTAG CCCTCAGCCC GACTGTTCAG 
AGTCCTGTCA CAGGGAGAAC TTTCCAGTAC AGTGACCCAT CCAGCGCCGC TGGCTCCCAC 
ACGTCCCTGC TCCTCCCGCA GTCCTCCTGT CCAGGCCAGC TCCCGGGTCA CCACTCCTCC 
GGGACGGACT CTCCTCTGGG CCGCTTTTAT GAGGATTTAA ACATACTGTC CAGCTCGCAC 
CCGTCTCTCG GCGTTGAGGG AGCGGGTCAG TCTTCCCCTG GTTATTTATC ACCGTATTTA 
TCACCGACAC TAACGCCCAA ACCCTGTAGC TCGGTTTCAG GGCACATGTC GAGCCCCGTA 
CAGACTTCTC CAGGCCCTCG AGGTCAGGCT CTTTCAGTAG GGACGTCCCC CGCACTTTCT 
CTCCCGCGCA CCTCAGATGG AGATCTAGAA CCGCTGAGAT CCAAACTCCC CTCCAATTTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_686078.4
CDS	941..4363
Translation	MDRLHSSMRKRLYSLPQHIGQKASIMGDAEDSDKDTRRKSIRMKPLPTSGEIKSGESAIMETDIGRPVKTSSNGDCRRFRGSLSSITSRHVHDSDVAEERRLITDGEVTPSEESPPGAAGGGAEPGAQSGGGGGEEAAGSSQHQGIPDQQAGFIKLEGIEQILPDDERYYQAGFMHRQFGAMLQPGVNKFSLRMFGSEKAVEREQERVKSAGFWIIHPYSDFRFYWDLTMLLLMVGNLIIIPVGITFFKDEHTPPWIVFNVVSDTFFLMDLVLNFRTGIVKEDNAEIILDPQQIKIKYLRSWFVVDFISSIPVDYIFLIVETRIDSDFYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAMVRIVNLIGMMLLLCHWDGCLQFLVPMLQDFPADCWVAKNKMVNDTWGQQYSYALFKAMSHMLCIGYGMYPPVGMTDVWLTILSMIVGATCYAMFVGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPADMRQRIHDYYEHRYQGKMFDEESILGELNEPLREEIINFNCRKLVASMPLFANADPNFVTSMLTKLRFEVFQPGDYIIREGTIGKKMYFIQHGVVSVLTKGNKETKLSDGSYFGEICLLTRGRRTASVRADTYCRLYSLSVDNFNEVLEEYPMMRRAFETVALDRLDRIGKKNSILQHKVQHDLNSGVLNYQESEIIQQIVQHDRDMAHCANLLQSPSPPAPPSPTPVIWAPLIQAPLQAAAATTSVAIALTHHPHLPATLFRPPVSLLGSRNEPPSRLKRFQTVAPRTGSTTDSPSTSPSKLHSGVDTPLLASLHTQHSSVTITPSATTNQPVSFRSFSSPSASPTLSTAQLHPQPRQKQPSTPPLSARLQAAGAHPPGILTTASSNTSALSAGLSTHSPTHSYPPPKHPQTGSLQFASGGGKSGLTLFHSPPPGSPISIHSQAPESSTSSQTQPSFSSYLALERSALASIAQYGSANASPSYTPLALSPTVQSPVTGRTFQYSDPSSAAGSHTSLLLPQSSCPGQLPGHHSSGTDSPLGRFYEDLNILSSSHPSLGVEGAGQSSPGYLSPYLSPTLTPKPCSSVSGHMSSPVQTSPGPRGQALSVGTSPALSLPRTSDGDLEPLRSKLPSNL

Target ORF information:

RefSeq Version	XM_680986.6
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio hyperpolarization activated cyclic nucleotide-gated potassium channel 4 (hcn4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_680986.6

ORF Insert Sequence:

ATGGACAGGT TGCATTCGTC CATGCGTAAA AGGCTCTACA GTTTGCCACA GCACATCGGG 
CAGAAAGCGT CCATCATGGG TGATGCTGAA GACTCAGACA AGGACACCCG GAGGAAAAGC 
ATCCGGATGA AGCCTTTACC GACCAGCGGG GAGATTAAAA GCGGAGAATC CGCGATCATG 
GAGACGGACA TCGGGAGACC CGTGAAGACC AGCTCCAACG GGGACTGCCG GAGGTTCCGC 
GGCAGCCTTT CGTCCATCAC CAGTCGGCAC GTCCACGACT CGGATGTAGC CGAGGAAAGG 
CGTCTGATCA CGGACGGAGA GGTCACTCCT AGCGAGGAGA GCCCTCCTGG AGCCGCGGGT 
GGCGGCGCGG AGCCCGGTGC GCAGAGCGGC GGGGGAGGCG GAGAGGAAGC CGCCGGTTCG 
AGCCAGCATC AGGGCATCCC GGATCAGCAG GCTGGCTTTA TTAAGCTGGA AGGCATCGAG 
CAGATACTGC CGGACGACGA GAGGTATTAT CAAGCGGGCT TCATGCACAG GCAGTTCGGG 
GCGATGCTGC AGCCGGGGGT CAACAAGTTT TCCCTGAGGA TGTTTGGGAG CGAGAAGGCG 
GTGGAGCGGG AGCAGGAACG GGTCAAATCC GCCGGCTTCT GGATTATTCA CCCCTACAGC 
GATTTCAGGT TTTACTGGGA TCTGACTATG CTGCTCCTGA TGGTGGGCAA TCTAATCATC 
ATCCCGGTCG GCATCACCTT CTTTAAAGAT GAGCACACGC CGCCGTGGAT CGTCTTTAAC 
GTGGTCTCCG ACACTTTCTT CCTGATGGAT CTGGTGCTGA ACTTCCGCAC GGGGATCGTA 
AAGGAGGACA ACGCAGAAAT CATCCTGGAC CCTCAGCAGA TCAAGATCAA GTATCTGCGC 
TCGTGGTTCG TGGTGGACTT CATCTCCTCC ATTCCCGTGG ACTATATCTT CCTCATAGTG 
GAGACGCGCA TCGATTCGGA TTTCTACAAG ACGGCGCGAG CTCTGCGGAT CGTCAGATTC 
ACTAAAATCC TCAGTCTGCT GCGGCTGCTG CGGCTCTCGC GCCTCATACG ATACATCCAC 
CAGTGGGAGG AGATTTTTCA TATGACCTAT GACCTGGCCA GTGCAATGGT GCGAATCGTC 
AACTTGATAG GGATGATGCT GCTGCTCTGC CACTGGGACG GATGTCTGCA GTTTCTGGTG 
CCCATGCTGC AAGACTTCCC TGCTGACTGC TGGGTGGCCA AAAACAAGAT GGTGAACGAC 
ACGTGGGGTC AGCAGTATTC CTACGCTCTC TTCAAGGCCA TGAGCCACAT GCTGTGTATA 
GGATATGGCA TGTATCCTCC CGTGGGCATG ACAGACGTGT GGTTGACCAT CCTCAGCATG 
ATCGTGGGTG CCACGTGTTA CGCCATGTTT GTTGGGCACG CCACAGCGCT GATCCAGTCC 
CTGGACTCCT CGCGCAGGCA GTATCAGGAG AAGTATAAGC AGGTGGAGCA GTACATGTCC 
TTCCATAAGC TGCCAGCAGA CATGAGACAG CGGATCCACG ATTACTACGA GCACAGATAC 
CAGGGCAAGA TGTTTGATGA AGAGAGCATT CTGGGAGAAC TCAATGAGCC ACTGAGAGAG 
GAGATCATAA ACTTCAACTG TCGGAAGCTG GTAGCGTCGA TGCCACTGTT CGCCAATGCA 
GATCCTAATT TTGTGACATC GATGCTCACT AAACTTCGCT TCGAGGTCTT CCAGCCGGGC 
GACTACATCA TACGAGAAGG AACCATCGGG AAGAAGATGT ACTTCATCCA GCACGGTGTG 
GTGAGCGTCC TTACCAAAGG AAACAAGGAG ACCAAGCTGT CGGACGGATC CTACTTTGGA 
GAGATCTGTC TGCTGACGCG GGGTAGACGC ACAGCCAGTG TCAGGGCCGA CACATACTGC 
CGTCTGTACT CTCTGTCAGT GGACAACTTC AACGAGGTGC TGGAGGAGTA TCCCATGATG 
AGGAGAGCGT TTGAGACTGT TGCCCTCGAC CGGCTCGACC GCATCGGAAA GAAGAACTCA 
ATTCTCCAGC ACAAAGTCCA GCACGACTTG AATTCAGGAG TGCTGAACTA CCAGGAAAGT 
GAGATCATCC AGCAGATCGT GCAGCATGAT CGGGACATGG CCCACTGTGC AAACCTGCTG 
CAGAGTCCGT CTCCGCCTGC CCCTCCGTCC CCGACTCCGG TCATCTGGGC TCCACTGATC 
CAGGCTCCTC TACAAGCCGC TGCAGCCACC ACATCGGTCG CTATTGCACT AACACACCAC 
CCACATCTAC CCGCCACGCT CTTCCGACCT CCCGTTTCCC TGCTCGGATC CAGAAACGAG 
CCTCCCAGCA GACTCAAGAG ATTTCAGACG GTTGCACCAA GAACAGGATC AACCACTGAC 
TCTCCATCCA CCTCCCCATC AAAGCTTCAT TCAGGAGTGG ATACTCCTCT TCTAGCATCC 
CTTCATACTC AACACTCATC AGTCACCATC ACTCCCAGTG CTACCACCAA TCAACCAGTG 
TCATTCAGAA GCTTCAGTTC TCCATCAGCA TCTCCGACGT TAAGCACGGC GCAGCTTCAT 
CCACAACCGA GACAAAAACA GCCGAGCACG CCCCCTCTAT CAGCACGCCT GCAAGCAGCA 
GGAGCACATC CGCCCGGCAT CCTCACTACA GCCTCATCAA ACACTAGTGC ACTTTCAGCA 
GGACTATCCA CTCATTCCCC TACTCACAGT TATCCTCCAC CAAAACACCC TCAAACGGGC 
TCCCTACAGT TCGCATCCGG AGGAGGGAAG AGTGGGTTAA CCCTGTTTCA CAGTCCGCCA 
CCCGGATCTC CAATCTCCAT CCATTCCCAA GCCCCCGAGA GCTCCACGTC ATCCCAGACG 
CAGCCCTCTT TTTCCAGTTA CCTTGCTTTG GAAAGGTCAG CGCTGGCCTC GATCGCTCAG 
TACGGCTCAG CGAACGCCTC GCCGAGCTAC ACGCCTTTAG CCCTCAGCCC GACTGTTCAG 
AGTCCTGTCA CAGGGAGAAC TTTCCAGTAC AGTGACCCAT CCAGCGCCGC TGGCTCCCAC 
ACGTCCCTGC TCCTCCCGCA GTCCTCCTGT CCAGGCCAGC TCCCGGGTCA CCACTCCTCC 
GGGACGGACT CTCCTCTGGG CCGCTTTTAT GAGGATTTAA ACATACTGTC CAGCTCGCAC 
CCGTCTCTCG GCGTTGAGGG AGCGGGTCAG TCTTCCCCTG GTTATTTATC ACCGTATTTA 
TCACCGACAC TAACGCCCAA ACCCTGTAGC TCGGTTTCAG GGCACATGTC GAGCCCCGTA 
CAGACTTCTC CAGGCCCTCG AGGTCAGGCT CTTTCAGTAG GGACGTCCCC CGCACTTTCT 
CTCCCGCGCA CCTCAGATGG AGATCTAGAA CCGCTGAGAT CCAAACTCCC CTCCAATTTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hcn4 ( XM_680986.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_686078.4 Danio rerio hyperpolarization activated cyclic nucleotide-gated potassium channel 4 (hcn4), mRNA.

Price & Availability↑

CloneID	ODa23006
Clone ID Related Accession (Same CDS sequence)	XM_680986.6 , XM_680986.7
Accession Version	XM_680986.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_680986.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGGT TGCATTCGTC CATGCGTAAA AGGCTCTACA GTTTGCCACA GCACATCGGG 
CAGAAAGCGT CCATCATGGG TGATGCTGAA GACTCAGACA AGGACACCCG GAGGAAAAGC 
ATCCGGATGA AGCCTTTACC GACCAGCGGG GAGATTAAAA GCGGAGAATC CGCGATCATG 
GAGACGGACA TCGGGAGACC CGTGAAGACC AGCTCCAACG GGGACTGCCG GAGGTTCCGC 
GGCAGCCTTT CGTCCATCAC CAGTCGGCAC GTCCACGACT CGGATGTAGC CGAGGAAAGG 
CGTCTGATCA CGGACGGAGA GGTCACTCCT AGCGAGGAGA GCCCTCCTGG AGCCGCGGGT 
GGCGGCGCGG AGCCCGGTGC GCAGAGCGGC GGGGGAGGCG GAGAGGAAGC CGCCGGTTCG 
AGCCAGCATC AGGGCATCCC GGATCAGCAG GCTGGCTTTA TTAAGCTGGA AGGCATCGAG 
CAGATACTGC CGGACGACGA GAGGTATTAT CAAGCGGGCT TCATGCACAG GCAGTTCGGG 
GCGATGCTGC AGCCGGGGGT CAACAAGTTT TCCCTGAGGA TGTTTGGGAG CGAGAAGGCG 
GTGGAGCGGG AGCAGGAACG GGTCAAATCC GCCGGCTTCT GGATTATTCA CCCCTACAGC 
GATTTCAGGT TTTACTGGGA TCTGACTATG CTGCTCCTGA TGGTGGGCAA TCTAATCATC 
ATCCCGGTCG GCATCACCTT CTTTAAAGAT GAGCACACGC CGCCGTGGAT CGTCTTTAAC 
GTGGTCTCCG ACACTTTCTT CCTGATGGAT CTGGTGCTGA ACTTCCGCAC GGGGATCGTA 
AAGGAGGACA ACGCAGAAAT CATCCTGGAC CCTCAGCAGA TCAAGATCAA GTATCTGCGC 
TCGTGGTTCG TGGTGGACTT CATCTCCTCC ATTCCCGTGG ACTATATCTT CCTCATAGTG 
GAGACGCGCA TCGATTCGGA TTTCTACAAG ACGGCGCGAG CTCTGCGGAT CGTCAGATTC 
ACTAAAATCC TCAGTCTGCT GCGGCTGCTG CGGCTCTCGC GCCTCATACG ATACATCCAC 
CAGTGGGAGG AGATTTTTCA TATGACCTAT GACCTGGCCA GTGCAATGGT GCGAATCGTC 
AACTTGATAG GGATGATGCT GCTGCTCTGC CACTGGGACG GATGTCTGCA GTTTCTGGTG 
CCCATGCTGC AAGACTTCCC TGCTGACTGC TGGGTGGCCA AAAACAAGAT GGTGAACGAC 
ACGTGGGGTC AGCAGTATTC CTACGCTCTC TTCAAGGCCA TGAGCCACAT GCTGTGTATA 
GGATATGGCA TGTATCCTCC CGTGGGCATG ACAGACGTGT GGTTGACCAT CCTCAGCATG 
ATCGTGGGTG CCACGTGTTA CGCCATGTTT GTTGGGCACG CCACAGCGCT GATCCAGTCC 
CTGGACTCCT CGCGCAGGCA GTATCAGGAG AAGTATAAGC AGGTGGAGCA GTACATGTCC 
TTCCATAAGC TGCCAGCAGA CATGAGACAG CGGATCCACG ATTACTACGA GCACAGATAC 
CAGGGCAAGA TGTTTGATGA AGAGAGCATT CTGGGAGAAC TCAATGAGCC ACTGAGAGAG 
GAGATCATAA ACTTCAACTG TCGGAAGCTG GTAGCGTCGA TGCCACTGTT CGCCAATGCA 
GATCCTAATT TTGTGACATC GATGCTCACT AAACTTCGCT TCGAGGTCTT CCAGCCGGGC 
GACTACATCA TACGAGAAGG AACCATCGGG AAGAAGATGT ACTTCATCCA GCACGGTGTG 
GTGAGCGTCC TTACCAAAGG AAACAAGGAG ACCAAGCTGT CGGACGGATC CTACTTTGGA 
GAGATCTGTC TGCTGACGCG GGGTAGACGC ACAGCCAGTG TCAGGGCCGA CACATACTGC 
CGTCTGTACT CTCTGTCAGT GGACAACTTC AACGAGGTGC TGGAGGAGTA TCCCATGATG 
AGGAGAGCGT TTGAGACTGT TGCCCTCGAC CGGCTCGACC GCATCGGAAA GAAGAACTCA 
ATTCTCCAGC ACAAAGTCCA GCACGACTTG AATTCAGGAG TGCTGAACTA CCAGGAAAGT 
GAGATCATCC AGCAGATCGT GCAGCATGAT CGGGACATGG CCCACTGTGC AAACCTGCTG 
CAGAGTCCGT CTCCGCCTGC CCCTCCGTCC CCGACTCCGG TCATCTGGGC TCCACTGATC 
CAGGCTCCTC TACAAGCCGC TGCAGCCACC ACATCGGTCG CTATTGCACT AACACACCAC 
CCACATCTAC CCGCCACGCT CTTCCGACCT CCCGTTTCCC TGCTCGGATC CAGAAACGAG 
CCTCCCAGCA GACTCAAGAG ATTTCAGACG GTTGCACCAA GAACAGGATC AACCACTGAC 
TCTCCATCCA CCTCCCCATC AAAGCTTCAT TCAGGAGTGG ATACTCCTCT TCTAGCATCC 
CTTCATACTC AACACTCATC AGTCACCATC ACTCCCAGTG CTACCACCAA TCAACCAGTG 
TCATTCAGAA GCTTCAGTTC TCCATCAGCA TCTCCGACGT TAAGCACGGC GCAGCTTCAT 
CCACAACCGA GACAAAAACA GCCGAGCACG CCCCCTCTAT CAGCACGCCT GCAAGCAGCA 
GGAGCACATC CGCCCGGCAT CCTCACTACA GCCTCATCAA ACACTAGTGC ACTTTCAGCA 
GGACTATCCA CTCATTCCCC TACTCACAGT TATCCTCCAC CAAAACACCC TCAAACGGGC 
TCCCTACAGT TCGCATCCGG AGGAGGGAAG AGTGGGTTAA CCCTGTTTCA CAGTCCGCCA 
CCCGGATCTC CAATCTCCAT CCATTCCCAA GCCCCCGAGA GCTCCACGTC ATCCCAGACG 
CAGCCCTCTT TTTCCAGTTA CCTTGCTTTG GAAAGGTCAG CGCTGGCCTC GATCGCTCAG 
TACGGCTCAG CGAACGCCTC GCCGAGCTAC ACGCCTTTAG CCCTCAGCCC GACTGTTCAG 
AGTCCTGTCA CAGGGAGAAC TTTCCAGTAC AGTGACCCAT CCAGCGCCGC TGGCTCCCAC 
ACGTCCCTGC TCCTCCCGCA GTCCTCCTGT CCAGGCCAGC TCCCGGGTCA CCACTCCTCC 
GGGACGGACT CTCCTCTGGG CCGCTTTTAT GAGGATTTAA ACATACTGTC CAGCTCGCAC 
CCGTCTCTCG GCGTTGAGGG AGCGGGTCAG TCTTCCCCTG GTTATTTATC ACCGTATTTA 
TCACCGACAC TAACGCCCAA ACCCTGTAGC TCGGTTTCAG GGCACATGTC GAGCCCCGTA 
CAGACTTCTC CAGGCCCTCG AGGTCAGGCT CTTTCAGTAG GGACGTCCCC CGCACTTTCT 
CTCCCGCGCA CCTCAGATGG AGATCTAGAA CCGCTGAGAT CCAAACTCCC CTCCAATTTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_686078.4
CDS	933..4355
Translation	MDRLHSSMRKRLYSLPQHIGQKASIMGDAEDSDKDTRRKSIRMKPLPTSGEIKSGESAIMETDIGRPVKTSSNGDCRRFRGSLSSITSRHVHDSDVAEERRLITDGEVTPSEESPPGAAGGGAEPGAQSGGGGGEEAAGSSQHQGIPDQQAGFIKLEGIEQILPDDERYYQAGFMHRQFGAMLQPGVNKFSLRMFGSEKAVEREQERVKSAGFWIIHPYSDFRFYWDLTMLLLMVGNLIIIPVGITFFKDEHTPPWIVFNVVSDTFFLMDLVLNFRTGIVKEDNAEIILDPQQIKIKYLRSWFVVDFISSIPVDYIFLIVETRIDSDFYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAMVRIVNLIGMMLLLCHWDGCLQFLVPMLQDFPADCWVAKNKMVNDTWGQQYSYALFKAMSHMLCIGYGMYPPVGMTDVWLTILSMIVGATCYAMFVGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPADMRQRIHDYYEHRYQGKMFDEESILGELNEPLREEIINFNCRKLVASMPLFANADPNFVTSMLTKLRFEVFQPGDYIIREGTIGKKMYFIQHGVVSVLTKGNKETKLSDGSYFGEICLLTRGRRTASVRADTYCRLYSLSVDNFNEVLEEYPMMRRAFETVALDRLDRIGKKNSILQHKVQHDLNSGVLNYQESEIIQQIVQHDRDMAHCANLLQSPSPPAPPSPTPVIWAPLIQAPLQAAAATTSVAIALTHHPHLPATLFRPPVSLLGSRNEPPSRLKRFQTVAPRTGSTTDSPSTSPSKLHSGVDTPLLASLHTQHSSVTITPSATTNQPVSFRSFSSPSASPTLSTAQLHPQPRQKQPSTPPLSARLQAAGAHPPGILTTASSNTSALSAGLSTHSPTHSYPPPKHPQTGSLQFASGGGKSGLTLFHSPPPGSPISIHSQAPESSTSSQTQPSFSSYLALERSALASIAQYGSANASPSYTPLALSPTVQSPVTGRTFQYSDPSSAAGSHTSLLLPQSSCPGQLPGHHSSGTDSPLGRFYEDLNILSSSHPSLGVEGAGQSSPGYLSPYLSPTLTPKPCSSVSGHMSSPVQTSPGPRGQALSVGTSPALSLPRTSDGDLEPLRSKLPSNL

Target ORF information:

RefSeq Version	XM_680986.7
Organism	Danio rerio(zebrafish)
Definition	Danio rerio hyperpolarization activated cyclic nucleotide-gated potassium channel 4 (hcn4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_680986.7

ORF Insert Sequence:

ATGGACAGGT TGCATTCGTC CATGCGTAAA AGGCTCTACA GTTTGCCACA GCACATCGGG 
CAGAAAGCGT CCATCATGGG TGATGCTGAA GACTCAGACA AGGACACCCG GAGGAAAAGC 
ATCCGGATGA AGCCTTTACC GACCAGCGGG GAGATTAAAA GCGGAGAATC CGCGATCATG 
GAGACGGACA TCGGGAGACC CGTGAAGACC AGCTCCAACG GGGACTGCCG GAGGTTCCGC 
GGCAGCCTTT CGTCCATCAC CAGTCGGCAC GTCCACGACT CGGATGTAGC CGAGGAAAGG 
CGTCTGATCA CGGACGGAGA GGTCACTCCT AGCGAGGAGA GCCCTCCTGG AGCCGCGGGT 
GGCGGCGCGG AGCCCGGTGC GCAGAGCGGC GGGGGAGGCG GAGAGGAAGC CGCCGGTTCG 
AGCCAGCATC AGGGCATCCC GGATCAGCAG GCTGGCTTTA TTAAGCTGGA AGGCATCGAG 
CAGATACTGC CGGACGACGA GAGGTATTAT CAAGCGGGCT TCATGCACAG GCAGTTCGGG 
GCGATGCTGC AGCCGGGGGT CAACAAGTTT TCCCTGAGGA TGTTTGGGAG CGAGAAGGCG 
GTGGAGCGGG AGCAGGAACG GGTCAAATCC GCCGGCTTCT GGATTATTCA CCCCTACAGC 
GATTTCAGGT TTTACTGGGA TCTGACTATG CTGCTCCTGA TGGTGGGCAA TCTAATCATC 
ATCCCGGTCG GCATCACCTT CTTTAAAGAT GAGCACACGC CGCCGTGGAT CGTCTTTAAC 
GTGGTCTCCG ACACTTTCTT CCTGATGGAT CTGGTGCTGA ACTTCCGCAC GGGGATCGTA 
AAGGAGGACA ACGCAGAAAT CATCCTGGAC CCTCAGCAGA TCAAGATCAA GTATCTGCGC 
TCGTGGTTCG TGGTGGACTT CATCTCCTCC ATTCCCGTGG ACTATATCTT CCTCATAGTG 
GAGACGCGCA TCGATTCGGA TTTCTACAAG ACGGCGCGAG CTCTGCGGAT CGTCAGATTC 
ACTAAAATCC TCAGTCTGCT GCGGCTGCTG CGGCTCTCGC GCCTCATACG ATACATCCAC 
CAGTGGGAGG AGATTTTTCA TATGACCTAT GACCTGGCCA GTGCAATGGT GCGAATCGTC 
AACTTGATAG GGATGATGCT GCTGCTCTGC CACTGGGACG GATGTCTGCA GTTTCTGGTG 
CCCATGCTGC AAGACTTCCC TGCTGACTGC TGGGTGGCCA AAAACAAGAT GGTGAACGAC 
ACGTGGGGTC AGCAGTATTC CTACGCTCTC TTCAAGGCCA TGAGCCACAT GCTGTGTATA 
GGATATGGCA TGTATCCTCC CGTGGGCATG ACAGACGTGT GGTTGACCAT CCTCAGCATG 
ATCGTGGGTG CCACGTGTTA CGCCATGTTT GTTGGGCACG CCACAGCGCT GATCCAGTCC 
CTGGACTCCT CGCGCAGGCA GTATCAGGAG AAGTATAAGC AGGTGGAGCA GTACATGTCC 
TTCCATAAGC TGCCAGCAGA CATGAGACAG CGGATCCACG ATTACTACGA GCACAGATAC 
CAGGGCAAGA TGTTTGATGA AGAGAGCATT CTGGGAGAAC TCAATGAGCC ACTGAGAGAG 
GAGATCATAA ACTTCAACTG TCGGAAGCTG GTAGCGTCGA TGCCACTGTT CGCCAATGCA 
GATCCTAATT TTGTGACATC GATGCTCACT AAACTTCGCT TCGAGGTCTT CCAGCCGGGC 
GACTACATCA TACGAGAAGG AACCATCGGG AAGAAGATGT ACTTCATCCA GCACGGTGTG 
GTGAGCGTCC TTACCAAAGG AAACAAGGAG ACCAAGCTGT CGGACGGATC CTACTTTGGA 
GAGATCTGTC TGCTGACGCG GGGTAGACGC ACAGCCAGTG TCAGGGCCGA CACATACTGC 
CGTCTGTACT CTCTGTCAGT GGACAACTTC AACGAGGTGC TGGAGGAGTA TCCCATGATG 
AGGAGAGCGT TTGAGACTGT TGCCCTCGAC CGGCTCGACC GCATCGGAAA GAAGAACTCA 
ATTCTCCAGC ACAAAGTCCA GCACGACTTG AATTCAGGAG TGCTGAACTA CCAGGAAAGT 
GAGATCATCC AGCAGATCGT GCAGCATGAT CGGGACATGG CCCACTGTGC AAACCTGCTG 
CAGAGTCCGT CTCCGCCTGC CCCTCCGTCC CCGACTCCGG TCATCTGGGC TCCACTGATC 
CAGGCTCCTC TACAAGCCGC TGCAGCCACC ACATCGGTCG CTATTGCACT AACACACCAC 
CCACATCTAC CCGCCACGCT CTTCCGACCT CCCGTTTCCC TGCTCGGATC CAGAAACGAG 
CCTCCCAGCA GACTCAAGAG ATTTCAGACG GTTGCACCAA GAACAGGATC AACCACTGAC 
TCTCCATCCA CCTCCCCATC AAAGCTTCAT TCAGGAGTGG ATACTCCTCT TCTAGCATCC 
CTTCATACTC AACACTCATC AGTCACCATC ACTCCCAGTG CTACCACCAA TCAACCAGTG 
TCATTCAGAA GCTTCAGTTC TCCATCAGCA TCTCCGACGT TAAGCACGGC GCAGCTTCAT 
CCACAACCGA GACAAAAACA GCCGAGCACG CCCCCTCTAT CAGCACGCCT GCAAGCAGCA 
GGAGCACATC CGCCCGGCAT CCTCACTACA GCCTCATCAA ACACTAGTGC ACTTTCAGCA 
GGACTATCCA CTCATTCCCC TACTCACAGT TATCCTCCAC CAAAACACCC TCAAACGGGC 
TCCCTACAGT TCGCATCCGG AGGAGGGAAG AGTGGGTTAA CCCTGTTTCA CAGTCCGCCA 
CCCGGATCTC CAATCTCCAT CCATTCCCAA GCCCCCGAGA GCTCCACGTC ATCCCAGACG 
CAGCCCTCTT TTTCCAGTTA CCTTGCTTTG GAAAGGTCAG CGCTGGCCTC GATCGCTCAG 
TACGGCTCAG CGAACGCCTC GCCGAGCTAC ACGCCTTTAG CCCTCAGCCC GACTGTTCAG 
AGTCCTGTCA CAGGGAGAAC TTTCCAGTAC AGTGACCCAT CCAGCGCCGC TGGCTCCCAC 
ACGTCCCTGC TCCTCCCGCA GTCCTCCTGT CCAGGCCAGC TCCCGGGTCA CCACTCCTCC 
GGGACGGACT CTCCTCTGGG CCGCTTTTAT GAGGATTTAA ACATACTGTC CAGCTCGCAC 
CCGTCTCTCG GCGTTGAGGG AGCGGGTCAG TCTTCCCCTG GTTATTTATC ACCGTATTTA 
TCACCGACAC TAACGCCCAA ACCCTGTAGC TCGGTTTCAG GGCACATGTC GAGCCCCGTA 
CAGACTTCTC CAGGCCCTCG AGGTCAGGCT CTTTCAGTAG GGACGTCCCC CGCACTTTCT 
CTCCCGCGCA CCTCAGATGG AGATCTAGAA CCGCTGAGAT CCAAACTCCC CTCCAATTTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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hcn4 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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