tulp4a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tulp4a
Entrez Gene ID	557730
Full Name	tubby like protein 4a
Synonyms	si:ch211-222h17.4,si:ch211-51e12.5,tulp4,wu:fb75h04,wu:fb99g11
General protein information	Preferred Names tubby like protein 4a Names tubby-related protein 4 fb99g11 tubby like protein 4
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005168512.4	XP_005168569.1	tubby-related protein 4 isoform X1
NM_001045095.1	NP_001038560.1	tubby-related protein 4
XM_005168512.3	XP_005168569.1	tubby-related protein 4 isoform X1
XM_017355027.1	XP_017210516.1	tubby-related protein 4 isoform X2
XM_017355027.2	XP_017210516.1	tubby-related protein 4 isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	TULP4	XP_005615601.1
Homo sapiens (human)	TULP4	NP_064630.2
Pan troglodytes (chimpanzee)	TULP4	XP_001145255.1
Rattus norvegicus (Norway rat)	Tulp4	NP_001102607.1
Danio rerio (zebrafish)	tulp4a	NP_001038560.1
Mus musculus (house mouse)	Tulp4	NP_473381.1
Gallus gallus (chicken)	TULP4	XP_419697.3
Danio rerio (zebrafish)	LOC562633	XP_691107.3
Xenopus tropicalis (tropical clawed frog)	tulp4	NP_001107316.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Efficient genome-wide mutagenesis of zebrafish genes by retroviral insertions.
Wang D, Jao LE, Zheng N, Dolan K, Ivey J, Zonies S, Wu X, Wu K, Yang H, Meng Q, Zhu Z, Zhang B, Lin S, Burgess SM
Proceedings of the National Academy of Sciences of the United States of America104(30)12428-33(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following tulp4a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tulp4a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa35738	NM_001045095.1 Latest version!	Danio rerio TUB like protein 4a (tulp4a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa08999	XM_005168512.4 Latest version!	Danio rerio tubby like protein 4a (tulp4a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa42658	XM_017355027.2 Latest version!	Danio rerio tubby like protein 4a (tulp4a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
ODa08999	XM_005168512.3	Danio rerio tubby like protein 4a (tulp4a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa42658	XM_017355027.1	Danio rerio tubby like protein 4a (tulp4a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tulp4a ( NM_001045095.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001038560.1 Danio rerio TUB like protein 4a (tulp4a), mRNA.

Price & Availability↑

CloneID	ODa35738
Clone ID Related Accession (Same CDS sequence)	NM_001045095.1
Accession Version	NM_001045095.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556380800000
Organism	Danio rerio(zebrafish)
Product	tubby-related protein 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX890562.8. On or before Nov 16, 2012 this sequence version replaced NM_001044838.2, XM_689281.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTTTGCGG CGGTGGAGCA CGGGCCCGTC CTGTGCAGCG ACTCCAACAT CCTGTGCCTG 
TCCTGGAAGG GCCGCGTGCC GCGCAGCCAG AAGGAGAAGC CGGTGTGTCG GCGACGGTAC 
TGCGAGGAGG GCTGGCTGGC CACGGGGAAC GGCAGAGGGG TGGTGGGAGT CACCTTCACC 
TCCAGCCACT GCAGGAGGGA CCGAAACACT CCACAGCGCA TCAACTTCAA CCTCAGGGGA 
CACAACAGTG AGGTGGTGCT GGTGCGCTGG AACGAGCCCT TCCAGAAGCT GGCGACCTGC 
GATACTGACG GTGGGATCTT CGTCTGGATT CAGTATGAGG GGCGCTGGTC GGTGGAGCTG 
GTCAATGATC GCGGAGCTCA GGTGAGCGAC TTCACCTGGT CTCATGATGG GACGCAGGCT 
CTGATCTCGT ACCGTGATGG TTTCGTGTTG GTGGGTTCGG TGAGCGGTCA GCGCCACTGG 
TCCTCCGAGA TCAACCTTGA GAGTCAGATC ACCTGCGGCA TCTGGACGCC CGACGACCAG 
CAGGTGTTGT TTGGCACTGC AGACGGGCAG GTGATCGTGA TGGACTGCCA CGGCCGCATG 
TTGGCTCATA TTCTGCTCCA TGAGGCCGAG GGCATCGCCA GCATGGCCTG GAACTATCCC 
AGCTTCCTGG TGGAGGACAG CAGCGAGAGC GACTCCGATT CAGACGACAG CACGCCTCCG 
CAGGTGCACA CCCTGAAGCC GCTGCTGACC GTCTGCTTCA CGTCCGGAGA CATCAGTTTG 
ATGAGCAATT ATGATGACCT TTCACCTCTG CTGATACGCA CGGGCCTCAA AGATGTGGTG 
GTGCAGTGGT CTTCCCAGGG GGATCTGCTG GCTGTCGCAG GGATGGAGAG ACTCGTGACT 
TCCGACCCCA CACAGGCCAC CCGAAACGCC ATTGTAAAGT TTTACAATGT TCGCGGGGAG 
CACATTTACT CACTGGAAAC ACCTGCTCAG AGGCCGATCA GTACGCTGTG CTGGGGCCAC 
CGGGACTCGC GTGTGTTTCT GGCCTGCGGG CCGGCGCTGT ATGTGCTGCG GGTCGAGCAC 
AGAGTCTCCA GCCTGCAGCT TCTGTGCCAA CAGGCCATCG CCAGTGCCGT ACACGAGGAG 
AAAGATCTGG CCAAGCTCAG CATGCCCTCC CGCCTCAGCA CATATGTGTC TTCTGCCTTC 
GCTCCCACTA TTAAGCCTCC AATCCCAGAT CCCAACAACA TGCGGGACTT TGTTAGCTAT 
CCGACGTCAG GTAATGAGCG TCTGCACTGC ACCATGAAGC GAACGGAGGA CAACCCTGAG 
GTCGGCGGTC CATGCTACAC CTTATACCTG GAGTATCTGG GAGGACTCGT TCCTATTCTG 
AAAGGCCGTA GGATAAGCAA ACTTCGTCCT GAATTTGTCA TCATGGACCC GAAGACTGAT 
GGGAAAACAG ATGAAGTGTA TGGGAACAGC TTCATCCCCT CCATGATTGA CAGCTGCAAT 
TGCTCAGATT CAAGTGACAT TGAGCTCAAT GATGATTGGG TAGGAAAGAA ATCACCAAAG 
ATCTCCAGAG GGAGCAAGTC TCCCAAACTT CCCAGAGACT TAGTGTGTCC CTTTACCAAC 
AGGATAAATA TAGATCCTCG CAAGTCCCCC AAGCTGTCCC GCACCACACA GGAAATATCC 
CGATCGCCAC GGTTACCCAT TCGCAAGCCC TCTATTGGCT CTCCAAGTCT GACTCGCAGA 
GAGTTCCCAC TTGATGACAT CACACAGCAC AATTACCTTG CGCAAGTTAC ATCTAACATA 
TGGGGAACAA AGTTCAAAAT TGTGGGACTT GCAGCATTTT TGCCAACAAA TCTTGGTGCA 
GTTATCTACA AGACAAGTCT GCTTCACCTC CAGCCCAGAC AGATGACCAT CTACCTCCCT 
GAGGTGAGGA AGATCTCGGT GGAATACATT AACCTTCCTA TGTTCAGTCC TAATGTCTTC 
AGTGAGGACG AGGACGACCT GCCTGTCACA GGAGCTTCAG GAATCACTGA TGATAATCCA 
CCTTGCACTG TTAACATACC CATTGCCCCC ATACACAGCC CTGCCCAAGC CATGTCCCCA 
ACTCAAAGCA TTGGCCTAGT CCAATCCCTA CTAGCCAATC AGAATGTCCA GTTAGACGTA 
TTGTCCAACC CAACATCGAC TGTGGCTCCT CCTGGGGTGT CTGATCAAGG ACAGCATGAC 
ACTGTGCTTA CGGCACAATA CACTGTTCCT GCCAGGTATT CTAGTCCTGG TCAGCTACAG 
CACCAGCAAA TGCAGCAGCA GTTGCAACAG CAGCATCAGA TGCAAATCCC TGTTCCAACT 
TTGCCCTCAG GTCAACCATC ATGCCCAGTC ATTCAGCTTC CACAAAGTAC AATACCAGTC 
GCTGCCCCTA CTGCAGAGCA CAGCACAGAG CGCGGGGAGC ATGAGCTCCT GCTTAAAATT 
AAAACCACTC GTTCTGCCCC ACAACTAGCT GAAGGTGATG CAGTGGTTTT TACAGCACCT 
CTTGAGATTA GCAAAATGAA CCCGCCCCCT CCTTACCCTG GCACAGTCGC CGCTGCAGTG 
GCAGCTGCTA CTGCTGCAGT TTCGGCTGCT GCTTCTGCAA GTTCCACCTC TGGGGCAGTG 
GGAAGCAGCT CTAGCACGTC ATCCTCAGGG GAAGTTTGTC TGAAGAAAGG GGATCTGACC 
TTGTATCCAC CTAGTGCACA AGGGGCTCAG TACCCAACTC CATTGGGCTA TGAACGCATC 
ACCACATTTG ACAGCAGTGG AAATGTGGAA GAGGTGTGCC GACCCCGCAC ACGACTTGTT 
TGCAACCAGA ATGTTTACAC GCTGCAGGGC CCAGGAAGCT CTGCCACCCT CAGGGTCTCA 
TCATCTTCCT CTGCTGTAGA GGGGAAGAAG GTGCAGCTGC CCTATGCCTC TGCTACGTTG 
AACCGTCTCA CTGTGCCAAG GTACTCTATA CCCAGTGGGG ACCCTCCTCC ATACCCTGAT 
ACAGCTAACC AGGGCGGAGC AGGGCGCAAC CCTGGCCAGA GACTGGACAG CAGCTTGATT 
CATGCTACCT TGAGGCGCAA CAGTCGGGAA GCTGCATTGA AGGTTTCCCA AATGCTGGAT 
CACCAGCGGA CTTTACCCCC CAAGGCTAAA GCCAGTACTT TGTCTGCTTC CTATCAACCA 
AGAGGGCCGA CAGCTCTTTA TACATGCAGT CAGTGCAGTA GCAACAGTAG TGGAACCACA 
AGCAGTGGAA GTAACGGAAT AGCTGGGGGC ACAATCATTA GGCAAGATTT CCCACCAACT 
GGGGGTGCTG CACCTCATAG CACTGTTATA GTGCACTCCA ACAGTGCCTC GCCTCTGCCT 
TCTCAATCCT CCTACAGTCT GCTCAGTCTG GATGGCACCG GTGGAGTGGC TACTGGAGCT 
GGAAACAGGG AAAGAGCTGA CTATATCAAC TCAGCTTTTA CAGAGGACGA GGCATTGAAT 
CAGTCTCTGA GGCAAATGGC CCTTAATACC GAGGTAGTGG GTTTGACAGT CAAACGGCCT 
CCACCGTATC AATGGGACCC ATCTGTTACA GAAGAGATTT GGGTTCCTCA AGAGCGCACA 
GCGACTCTGC CCCCTTCTGG ACCTCCTGCA CCTCACAAAC CTCCTCCGCT CATCCTCGGT 
CCTGCACAGC ATCTGGACGT TTCTCGTCTT CCGTTTGTCC TCTCACCTAA ATCTCCAACA 
AGTCCTAACA ACACCACTTT TCCACTAGCA GGAGCTTATC AGATTGCATT GCCCTATCCA 
TCAAGTGCTG CATACAGTAG TCCCCCACTG CAGGCAGTGC AAGCATCTCC ACGGCCGCCT 
TCTCCTAAAG AGGCGGTTGC TCCACTTTCC TTTCCCCAGC AAGACCAGTC AGTGGTCTTG 
CCTCCTGGTT ATCCACCAAA TTTGCCCAAC CTGGCTTGCT GTCCCCTCCC ACCAATGTAC 
CCTGGCACAG CTTCTTGTTC TAGCCTGCCT GTAACCGCCA TTCCTCTTCA TCCATGGGGT 
TCTTACAATC CCTGTCCCCC CATGCCAAGC CCCCCGGGTC CCGCCCCATC TCTCCCACCC 
AAGGCCTCCC ACATGCTGGA TAAACCTGTT CGTTCTCCAC CTCCTCAACC GCCGCCTTTG 
CCTTCGCCTC CCCCAGACCT CCAGACTGCG GTCAGTCCCC CAGAAGCAAT AGCTGAAGCC 
GGGGAAGGCT TTCAAGAGGT GCTCTCGCTT AACGAAAGCC CTGTTCCTCA ACGAGCAGAC 
AGGTTTGGCA AGAAGAATCG AAAGCGTTTG GACAACACTC GGGCAGATGA AGCTAATGTT 
CCAGCAATCA CAGAGGGAGG CAACAAGTCC AAAAAAGAGG GCCGTGCCCT TGGGGACTTC 
AACTCCCTCA TCTCCAGTCC GAGGTTAGGT GGGAGAGATA AAAAGAAACC TAAAGGTCAA 
AAAGAGCAGC TGAAAGCCAA AAAGCTAAGC AAGGCCACCA CAAATGAGTT CCAGGACAGC 
TCAGAGAGCG AGCCTGAGCT CTTTATCAGT GGAGATGAGC TGATGAACCA GAGCCAGAGT 
GGGAAGAAGA GTTGGAAAGC CAAAAGGAAC CTGCGGGCAG GAGGTTGTGA GCTGGATGAG 
ATCAAATGTC GAAAAGCGAA TGAGAAAGAG GACCGAGGCC TCGGCAGCCA GGGCTTCGTC 
TACATCATGG CCAACAAACA GCCTCTCTGG AATGAAGCCA CGCAGGTCTA CCAGCTAGAC 
TTTGGAGGGA GAGTCACACA GGAATCAGCA AAGAACTTCC AGATTGAGCT TGAGGGGAGA 
CAGGTGATGC AGTTCGGAAG GATCGACGGG AACGCATATA TCCTGGACTT TCAGTACCCC 
TTCTCAGCTG TGCAAGCTTT TGCTGTAGCA CTTGCTAATG TCACCCAGCG CCTCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001038560.1
CDS	1..4920
Translation	MFAAVEHGPVLCSDSNILCLSWKGRVPRSQKEKPVCRRRYCEEGWLATGNGRGVVGVTFTSSHCRRDRNTPQRINFNLRGHNSEVVLVRWNEPFQKLATCDTDGGIFVWIQYEGRWSVELVNDRGAQVSDFTWSHDGTQALISYRDGFVLVGSVSGQRHWSSEINLESQITCGIWTPDDQQVLFGTADGQVIVMDCHGRMLAHILLHEAEGIASMAWNYPSFLVEDSSESDSDSDDSTPPQVHTLKPLLTVCFTSGDISLMSNYDDLSPLLIRTGLKDVVVQWSSQGDLLAVAGMERLVTSDPTQATRNAIVKFYNVRGEHIYSLETPAQRPISTLCWGHRDSRVFLACGPALYVLRVEHRVSSLQLLCQQAIASAVHEEKDLAKLSMPSRLSTYVSSAFAPTIKPPIPDPNNMRDFVSYPTSGNERLHCTMKRTEDNPEVGGPCYTLYLEYLGGLVPILKGRRISKLRPEFVIMDPKTDGKTDEVYGNSFIPSMIDSCNCSDSSDIELNDDWVGKKSPKISRGSKSPKLPRDLVCPFTNRINIDPRKSPKLSRTTQEISRSPRLPIRKPSIGSPSLTRREFPLDDITQHNYLAQVTSNIWGTKFKIVGLAAFLPTNLGAVIYKTSLLHLQPRQMTIYLPEVRKISVEYINLPMFSPNVFSEDEDDLPVTGASGITDDNPPCTVNIPIAPIHSPAQAMSPTQSIGLVQSLLANQNVQLDVLSNPTSTVAPPGVSDQGQHDTVLTAQYTVPARYSSPGQLQHQQMQQQLQQQHQMQIPVPTLPSGQPSCPVIQLPQSTIPVAAPTAEHSTERGEHELLLKIKTTRSAPQLAEGDAVVFTAPLEISKMNPPPPYPGTVAAAVAAATAAVSAAASASSTSGAVGSSSSTSSSGEVCLKKGDLTLYPPSAQGAQYPTPLGYERITTFDSSGNVEEVCRPRTRLVCNQNVYTLQGPGSSATLRVSSSSSAVEGKKVQLPYASATLNRLTVPRYSIPSGDPPPYPDTANQGGAGRNPGQRLDSSLIHATLRRNSREAALKVSQMLDHQRTLPPKAKASTLSASYQPRGPTALYTCSQCSSNSSGTTSSGSNGIAGGTIIRQDFPPTGGAAPHSTVIVHSNSASPLPSQSSYSLLSLDGTGGVATGAGNRERADYINSAFTEDEALNQSLRQMALNTEVVGLTVKRPPPYQWDPSVTEEIWVPQERTATLPPSGPPAPHKPPPLILGPAQHLDVSRLPFVLSPKSPTSPNNTTFPLAGAYQIALPYPSSAAYSSPPLQAVQASPRPPSPKEAVAPLSFPQQDQSVVLPPGYPPNLPNLACCPLPPMYPGTASCSSLPVTAIPLHPWGSYNPCPPMPSPPGPAPSLPPKASHMLDKPVRSPPPQPPPLPSPPPDLQTAVSPPEAIAEAGEGFQEVLSLNESPVPQRADRFGKKNRKRLDNTRADEANVPAITEGGNKSKKEGRALGDFNSLISSPRLGGRDKKKPKGQKEQLKAKKLSKATTNEFQDSSESEPELFISGDELMNQSQSGKKSWKAKRNLRAGGCELDEIKCRKANEKEDRGLGSQGFVYIMANKQPLWNEATQVYQLDFGGRVTQESAKNFQIELEGRQVMQFGRIDGNAYILDFQYPFSAVQAFAVALANVTQRLK

Target ORF information:

RefSeq Version	NM_001045095.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio TUB like protein 4a (tulp4a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001045095.1

ORF Insert Sequence:

ATGTTTGCGG CGGTGGAGCA CGGGCCCGTC CTGTGCAGCG ACTCCAACAT CCTGTGCCTG 
TCCTGGAAGG GCCGCGTGCC GCGCAGCCAG AAGGAGAAGC CGGTGTGTCG GCGACGGTAC 
TGCGAGGAGG GCTGGCTGGC CACGGGGAAC GGCAGAGGGG TGGTGGGAGT CACCTTCACC 
TCCAGCCACT GCAGGAGGGA CCGAAACACT CCACAGCGCA TCAACTTCAA CCTCAGGGGA 
CACAACAGTG AGGTGGTGCT GGTGCGCTGG AACGAGCCCT TCCAGAAGCT GGCGACCTGC 
GATACTGACG GTGGGATCTT CGTCTGGATT CAGTATGAGG GGCGCTGGTC GGTGGAGCTG 
GTCAATGATC GCGGAGCTCA GGTGAGCGAC TTCACCTGGT CTCATGATGG GACGCAGGCT 
CTGATCTCGT ACCGTGATGG TTTCGTGTTG GTGGGTTCGG TGAGCGGTCA GCGCCACTGG 
TCCTCCGAGA TCAACCTTGA GAGTCAGATC ACCTGCGGCA TCTGGACGCC CGACGACCAG 
CAGGTGTTGT TTGGCACTGC AGACGGGCAG GTGATCGTGA TGGACTGCCA CGGCCGCATG 
TTGGCTCATA TTCTGCTCCA TGAGGCCGAG GGCATCGCCA GCATGGCCTG GAACTATCCC 
AGCTTCCTGG TGGAGGACAG CAGCGAGAGC GACTCCGATT CAGACGACAG CACGCCTCCG 
CAGGTGCACA CCCTGAAGCC GCTGCTGACC GTCTGCTTCA CGTCCGGAGA CATCAGTTTG 
ATGAGCAATT ATGATGACCT TTCACCTCTG CTGATACGCA CGGGCCTCAA AGATGTGGTG 
GTGCAGTGGT CTTCCCAGGG GGATCTGCTG GCTGTCGCAG GGATGGAGAG ACTCGTGACT 
TCCGACCCCA CACAGGCCAC CCGAAACGCC ATTGTAAAGT TTTACAATGT TCGCGGGGAG 
CACATTTACT CACTGGAAAC ACCTGCTCAG AGGCCGATCA GTACGCTGTG CTGGGGCCAC 
CGGGACTCGC GTGTGTTTCT GGCCTGCGGG CCGGCGCTGT ATGTGCTGCG GGTCGAGCAC 
AGAGTCTCCA GCCTGCAGCT TCTGTGCCAA CAGGCCATCG CCAGTGCCGT ACACGAGGAG 
AAAGATCTGG CCAAGCTCAG CATGCCCTCC CGCCTCAGCA CATATGTGTC TTCTGCCTTC 
GCTCCCACTA TTAAGCCTCC AATCCCAGAT CCCAACAACA TGCGGGACTT TGTTAGCTAT 
CCGACGTCAG GTAATGAGCG TCTGCACTGC ACCATGAAGC GAACGGAGGA CAACCCTGAG 
GTCGGCGGTC CATGCTACAC CTTATACCTG GAGTATCTGG GAGGACTCGT TCCTATTCTG 
AAAGGCCGTA GGATAAGCAA ACTTCGTCCT GAATTTGTCA TCATGGACCC GAAGACTGAT 
GGGAAAACAG ATGAAGTGTA TGGGAACAGC TTCATCCCCT CCATGATTGA CAGCTGCAAT 
TGCTCAGATT CAAGTGACAT TGAGCTCAAT GATGATTGGG TAGGAAAGAA ATCACCAAAG 
ATCTCCAGAG GGAGCAAGTC TCCCAAACTT CCCAGAGACT TAGTGTGTCC CTTTACCAAC 
AGGATAAATA TAGATCCTCG CAAGTCCCCC AAGCTGTCCC GCACCACACA GGAAATATCC 
CGATCGCCAC GGTTACCCAT TCGCAAGCCC TCTATTGGCT CTCCAAGTCT GACTCGCAGA 
GAGTTCCCAC TTGATGACAT CACACAGCAC AATTACCTTG CGCAAGTTAC ATCTAACATA 
TGGGGAACAA AGTTCAAAAT TGTGGGACTT GCAGCATTTT TGCCAACAAA TCTTGGTGCA 
GTTATCTACA AGACAAGTCT GCTTCACCTC CAGCCCAGAC AGATGACCAT CTACCTCCCT 
GAGGTGAGGA AGATCTCGGT GGAATACATT AACCTTCCTA TGTTCAGTCC TAATGTCTTC 
AGTGAGGACG AGGACGACCT GCCTGTCACA GGAGCTTCAG GAATCACTGA TGATAATCCA 
CCTTGCACTG TTAACATACC CATTGCCCCC ATACACAGCC CTGCCCAAGC CATGTCCCCA 
ACTCAAAGCA TTGGCCTAGT CCAATCCCTA CTAGCCAATC AGAATGTCCA GTTAGACGTA 
TTGTCCAACC CAACATCGAC TGTGGCTCCT CCTGGGGTGT CTGATCAAGG ACAGCATGAC 
ACTGTGCTTA CGGCACAATA CACTGTTCCT GCCAGGTATT CTAGTCCTGG TCAGCTACAG 
CACCAGCAAA TGCAGCAGCA GTTGCAACAG CAGCATCAGA TGCAAATCCC TGTTCCAACT 
TTGCCCTCAG GTCAACCATC ATGCCCAGTC ATTCAGCTTC CACAAAGTAC AATACCAGTC 
GCTGCCCCTA CTGCAGAGCA CAGCACAGAG CGCGGGGAGC ATGAGCTCCT GCTTAAAATT 
AAAACCACTC GTTCTGCCCC ACAACTAGCT GAAGGTGATG CAGTGGTTTT TACAGCACCT 
CTTGAGATTA GCAAAATGAA CCCGCCCCCT CCTTACCCTG GCACAGTCGC CGCTGCAGTG 
GCAGCTGCTA CTGCTGCAGT TTCGGCTGCT GCTTCTGCAA GTTCCACCTC TGGGGCAGTG 
GGAAGCAGCT CTAGCACGTC ATCCTCAGGG GAAGTTTGTC TGAAGAAAGG GGATCTGACC 
TTGTATCCAC CTAGTGCACA AGGGGCTCAG TACCCAACTC CATTGGGCTA TGAACGCATC 
ACCACATTTG ACAGCAGTGG AAATGTGGAA GAGGTGTGCC GACCCCGCAC ACGACTTGTT 
TGCAACCAGA ATGTTTACAC GCTGCAGGGC CCAGGAAGCT CTGCCACCCT CAGGGTCTCA 
TCATCTTCCT CTGCTGTAGA GGGGAAGAAG GTGCAGCTGC CCTATGCCTC TGCTACGTTG 
AACCGTCTCA CTGTGCCAAG GTACTCTATA CCCAGTGGGG ACCCTCCTCC ATACCCTGAT 
ACAGCTAACC AGGGCGGAGC AGGGCGCAAC CCTGGCCAGA GACTGGACAG CAGCTTGATT 
CATGCTACCT TGAGGCGCAA CAGTCGGGAA GCTGCATTGA AGGTTTCCCA AATGCTGGAT 
CACCAGCGGA CTTTACCCCC CAAGGCTAAA GCCAGTACTT TGTCTGCTTC CTATCAACCA 
AGAGGGCCGA CAGCTCTTTA TACATGCAGT CAGTGCAGTA GCAACAGTAG TGGAACCACA 
AGCAGTGGAA GTAACGGAAT AGCTGGGGGC ACAATCATTA GGCAAGATTT CCCACCAACT 
GGGGGTGCTG CACCTCATAG CACTGTTATA GTGCACTCCA ACAGTGCCTC GCCTCTGCCT 
TCTCAATCCT CCTACAGTCT GCTCAGTCTG GATGGCACCG GTGGAGTGGC TACTGGAGCT 
GGAAACAGGG AAAGAGCTGA CTATATCAAC TCAGCTTTTA CAGAGGACGA GGCATTGAAT 
CAGTCTCTGA GGCAAATGGC CCTTAATACC GAGGTAGTGG GTTTGACAGT CAAACGGCCT 
CCACCGTATC AATGGGACCC ATCTGTTACA GAAGAGATTT GGGTTCCTCA AGAGCGCACA 
GCGACTCTGC CCCCTTCTGG ACCTCCTGCA CCTCACAAAC CTCCTCCGCT CATCCTCGGT 
CCTGCACAGC ATCTGGACGT TTCTCGTCTT CCGTTTGTCC TCTCACCTAA ATCTCCAACA 
AGTCCTAACA ACACCACTTT TCCACTAGCA GGAGCTTATC AGATTGCATT GCCCTATCCA 
TCAAGTGCTG CATACAGTAG TCCCCCACTG CAGGCAGTGC AAGCATCTCC ACGGCCGCCT 
TCTCCTAAAG AGGCGGTTGC TCCACTTTCC TTTCCCCAGC AAGACCAGTC AGTGGTCTTG 
CCTCCTGGTT ATCCACCAAA TTTGCCCAAC CTGGCTTGCT GTCCCCTCCC ACCAATGTAC 
CCTGGCACAG CTTCTTGTTC TAGCCTGCCT GTAACCGCCA TTCCTCTTCA TCCATGGGGT 
TCTTACAATC CCTGTCCCCC CATGCCAAGC CCCCCGGGTC CCGCCCCATC TCTCCCACCC 
AAGGCCTCCC ACATGCTGGA TAAACCTGTT CGTTCTCCAC CTCCTCAACC GCCGCCTTTG 
CCTTCGCCTC CCCCAGACCT CCAGACTGCG GTCAGTCCCC CAGAAGCAAT AGCTGAAGCC 
GGGGAAGGCT TTCAAGAGGT GCTCTCGCTT AACGAAAGCC CTGTTCCTCA ACGAGCAGAC 
AGGTTTGGCA AGAAGAATCG AAAGCGTTTG GACAACACTC GGGCAGATGA AGCTAATGTT 
CCAGCAATCA CAGAGGGAGG CAACAAGTCC AAAAAAGAGG GCCGTGCCCT TGGGGACTTC 
AACTCCCTCA TCTCCAGTCC GAGGTTAGGT GGGAGAGATA AAAAGAAACC TAAAGGTCAA 
AAAGAGCAGC TGAAAGCCAA AAAGCTAAGC AAGGCCACCA CAAATGAGTT CCAGGACAGC 
TCAGAGAGCG AGCCTGAGCT CTTTATCAGT GGAGATGAGC TGATGAACCA GAGCCAGAGT 
GGGAAGAAGA GTTGGAAAGC CAAAAGGAAC CTGCGGGCAG GAGGTTGTGA GCTGGATGAG 
ATCAAATGTC GAAAAGCGAA TGAGAAAGAG GACCGAGGCC TCGGCAGCCA GGGCTTCGTC 
TACATCATGG CCAACAAACA GCCTCTCTGG AATGAAGCCA CGCAGGTCTA CCAGCTAGAC 
TTTGGAGGGA GAGTCACACA GGAATCAGCA AAGAACTTCC AGATTGAGCT TGAGGGGAGA 
CAGGTGATGC AGTTCGGAAG GATCGACGGG AACGCATATA TCCTGGACTT TCAGTACCCC 
TTCTCAGCTG TGCAAGCTTT TGCTGTAGCA CTTGCTAATG TCACCCAGCG CCTCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tulp4a ( XM_005168512.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005168569.1 Danio rerio tubby like protein 4a (tulp4a), transcript variant X1, mRNA.

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CloneID	ODa08999
Clone ID Related Accession (Same CDS sequence)	XM_005168512.4 , XM_005168512.3
Accession Version	XM_005168512.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	tubby-related protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005168512.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGCGG CGGTGGAGCA CGGGCCCGTC CTGTGCAGCG ACTCCAACAT CCTGTGCCTG 
TCCTGGAAGG GCCGCGTGCC GCGCAGCCAG AAGGAGAAGC CGGTGTGTCG GCGACGGTAC 
TGCGAGGAGG GCTGGCTGGC CACGGGGAAC GGCAGAGGGG TGGTGGGAGT CACCTTCACC 
TCCAGCCACT GCAGGAGGGA CCGAAACACT CCACAGCGCA TCAACTTCAA CCTCAGGGGA 
CACAACAGTG AGGTGGTGCT GGTGCGCTGG AACGAGCCCT TCCAGAAGCT GGCGACCTGC 
GATACTGACG GTGGGATCTT CGTCTGGATT CAGTATGAGG GGCGCTGGTC GGTGGAGCTG 
GTCAATGATC GCGGAGCTCA GGTGAGCGAC TTCACCTGGT CTCATGATGG GACGCAGGCT 
CTGATCTCGT ACCGTGATGG TTTCGTGTTG GTGGGTTCGG TGAGCGGTCA GCGCCACTGG 
TCCTCCGAGA TCAACCTTGA GAGTCAGATC ACCTGCGGCA TCTGGACGCC CGACGACCAG 
CAGGTGTTGT TTGGCACTGC AGACGGGCAG GTGATCGTGA TGGACTGCCA CGGCCGCATG 
TTGGCTCATA TTCTGCTCCA TGAGGCCGAG GGCATCGCCA GCATGGCCTG GAACTATCCC 
AGCTTCCTGG TGGAGGACAG CAGCGAGAGC GACTCCGATT CAGACGACAG CACGCCTCCG 
CAGGTGCACA CCCTGAAGCC GCTGCTGACC GTCTGCTTCA CGTCCGGAGA CATCAGTTTG 
ATGAGCAATT ATGATGACCT TTCACCTCTG CTGATACGCA CGGGCCTCAA AGATGTGGTG 
GTGCAGTGGT CTTCCCAGGG GGATCTGCTG GCTGTCGCAG GGATGGAGAG ACTCGTGACT 
TCCGACCCCA CACAGGCCAC CCGAAACGCC ATTGTAAAGT TTTACAATGT TCGCGGGGAG 
CACATTTACT CACTGGAAAC ACCTGCTCAG AGGCCGATCA GTACGCTGTG CTGGGGCCAC 
CGGGACTCGC GTGTGTTTCT GGCCTGCGGG CCGGCGCTGT ATGTGCTGCG GGTCGAGCAC 
AGAGTCTCCA GCCTGCAGCT TCTGTGCCAA CAGGCCATCG CCAGTGCCGT ACACGAGGAG 
AAAGATCTGG CCAAGCTCAG CATGCCCTCC CGCCTCAGCA CATATGTGTC TTCTGCCTTC 
GCTCCCACTA TTAAGCCTCC AATCCCAGAT CCCAACAACA TGCGGGACTT TGTTAGCTAT 
CCGACGTCAG GTAATGAGCG TCTGCACTGC ACCATGAAGC GAACGGAGGA CAACCCTGAG 
GTCGGCGGTC CATGCTACAC CTTATACCTG GAGTATCTGG GAGGACTCGT TCCTATTCTG 
AAAGGCCGTA GGATAAGCAA ACTTCGTCCT GAATTTGTCA TCATGGACCC GAAGACTGAT 
GGGAAAACAG ATGAAGTGTA TGGGAACAGC TTCATCCCCT CCATGATTGA CAGCTGCAAT 
TGCTCAGATT CAAGTGACAT TGAGCTCAAT GATGATTGGG TAGGAAAGAA ATCACCAAAG 
ATCTCCAGAG GGAGCAAGTC TCCCAAACTT CCCAGAGACT TAGTGTGTCC CTTTACCAAC 
AGGATAAATA TAGATCCTCG CAAGTCCCCC AAGCTGTCCC GCACCACACA GGAAATATCC 
CGATCGCCAC GGTTACCCAT TCGCAAGCCC TCTATTGGCT CTCCAAGTCT GACTCGCAGA 
GAGTTCCCAC TTGATGACAT CACACAGCAC AATTACCTTG CGCAAGTTAC ATCTAACATA 
TGGGGAACAA AGTTCAAAAT TGTGGGACTT GCAGCATTTT TGCCAACAAA TCTTGGTGCA 
GTTATCTACA AGACAAGTCT GCTTCACCTC CAGCCCAGAC AGATGACCAT CTACCTCCCT 
GAGGTGAGGA AGATCTCGGT GGAATACATT AACCTTCCTA TGTTCAGTCC TAATGTCTTC 
AGTGAGGACG AGGACGACCT GCCTGTCACA GGAGCTTCAG GAATCACTGA TGATAATCCA 
CCTTGCACTG TTAACATACC CATTGCCCCC ATACACAGCC CTGCCCAAGC CATGTCCCCA 
ACTCAAAGCA TTGGCCTAGT CCAATCCCTA CTAGCCAATC AGAATGTCCA GTTAGACGTA 
TTGTCCAACC CAACATCGAC TGTGGCTCCT CCTGGGGTGT CTGATCAAGG ACAGCATGAC 
ACTGTGCTTA CGGCACAATA CACTGTTCCT GCCAGGTATT CTAGTCCTGG TCAGGTAATC 
TTCGGAGGTA TGGATGTTGG TCGGCTTTTA GTTGCACAGT CCCAACAGCA GCAGCAGCAA 
TCACAACAAC ATCAGCAGCT GCAACAGCAT TATCAGCAAC AGCAGCTACA GCAACAGCTG 
CAACAATATC AGCTGCAGCA GCAACAGCTG TTGCAGCATC AGCACATGCA GCAACAGCAG 
CAGCAACACA TGCAGCAGCA GATCCAACAG ATGCAGCAAC AGCAGCAGAT GCAGCAACAG 
CTTCAGCATC AAATACAGCA GCAGCATCTG CAAATGCAAC TGCAGCTACA GCACCAGCAA 
ATGCAGCAGC AGTTGCAACA GCAGCATCAG ATGCAAATCC CTGTTCCAAC TTTGCCCTCA 
GGTCAACCAT CATGCCCAGT CATTCAGCTT CCACAAAGTA CAATACCAGT CGCTGCCCCT 
ACTGCAGAGC ACAGCACAGA GCGCGGGGAG CATGAGCTCC TGCTTAAAAT TAAAACCACT 
CGTTCTGCCC CACAACTAGC TGAAGGTGAT GCAGTGGTTT TTACAGCACC TCTTGAGATT 
AGCAAAATGA ACCCGCCCCC TCCTTACCCT GGCACAGTCG CCGCTGCAGT GGCAGCTGCT 
ACTGCTGCAG TTTCGGCTGC TGCTTCTGCA AGTTCCACCT CTGGGGCAGT GGGAAGCAGC 
TCTAGCACGT CATCCTCAGG GGAAGTTTGT CTGAAGAAAG GGGATCTGAC CTTGTATCCA 
CCTAGTGCAC AAGGGGCTCA GTACCCAACT CCATTGGGCT ATGAACGCAT CACCACATTT 
GACAGCAGTG GAAATGTGGA AGAGGTGTGC CGACCCCGCA CACGACTTGT TTGCAACCAG 
AATGTTTACA CGCTGCAGGG CCCAGGAAGC TCTGCCACCC TCAGGGTCTC ATCATCTTCC 
TCTGCTGTAG AGGGGAAGAA GGTGCAGCTG CCCTATGCCT CTGCTACGTT GAACCGTCTC 
ACTGTGCCAA GGTACTCTAT ACCCAGTGGG GACCCTCCTC CATACCCTGA TACAGCTAAC 
CAGGGCGGAG CAGGGCGCAA CCCTGGCCAG AGACTGGACA GCAGCTTGAT TCATGCTACC 
TTGAGGCGCA ACAGTCGGGA AGCTGCATTG AAGGTTTCCC AAATGCTGGA TCACCAGCGG 
ACTTTACCCC CCAAGGCTAA AGCCAGTACT TTGTCTGCTT CCTATCAACC AAGAGGGCCG 
ACAGCTCTTT ATACATGCAG TCAGTGCAGT AGCAACAGTA GTGGAACCAC AAGCAGTGGA 
AGTAACGGAA TAGCTGGGGG CACAATCATT AGGCAAGATT TCCCACCAAC TGGGGGTGCT 
GCACCTCATA GCACTGTTAT AGTGCACTCC AACAGTGCCT CGCCTCTGCC TTCTCAATCC 
TCCTACAGTC TGCTCAGTCT GGATGGCACC GGTGGAGTGG CTACTGGAGC TGGAAACAGG 
GAAAGAGCTG ACTATATCAA CTCAGCTTTT ACAGAGGACG AGGCATTGAA TCAGTCTCTG 
AGGCAAATGG CCCTTAATAC CGAGGTAGTG GGTTTGACAG TCAAACGGCC TCCACCGTAT 
CAATGGGACC CATCTGTTAC AGAAGAGATT TGGGTTCCTC AAGAGCGCAC AGCGACTCTG 
CCCCCTTCTG GACCTCCTGC ACCTCACAAA CCTCCTCCGC TCATCCTCGG TCCTGCACAG 
CATCTGGACG TTTCTCGTCT TCCGTTTGTC CTCTCACCTA AATCTCCAAC AAGTCCTAAC 
AACACCACTT TTCCACTAGC AGGAGCTTAT CAGATTGCAT TGCCCTATCC ATCAAGTGCT 
GCATACAGTA GTCCCCCACT GCAGGCAGTG CAAGCATCTC CACGGCCGCC TTCTCCTAAA 
GAGGCGGTTG CTCCACTTTC CTTTCCCCAG CAAGACCAGT CAGTGGTCTT GCCTCCTGGT 
TATCCACCAA ATTTGCCCAA CCTGGCTTGC TGTCCCCTCC CACCAATGTA CCCTGGCACA 
GCTTCTTGTT CTAGCCTGCC TGTAACCGCC ATTCCTCTTC ATCCATGGGG TTCTTACAAT 
CCCTGTCCCC CCATGCCAAG CCCCCCGGGT CCCGCCCCAT CTCTCCCACC CAAGGCCTCC 
CACATGCTGG ATAAACCTGT TCGTTCTCCA CCTCCTCAAC CGCCGCCTTT GCCTTCGCCT 
CCCCCAGACC TCCAGACTGC GGTCAGTCCC CCAGAAGCAA TAGCTGAAGC CGGGGAAGGC 
TTTCAAGAGG TGCTCTCGCT TAACGAAAGC CCTGTTCCTC AACGAGCAGA CAGGTTTGGC 
AAGAAGAATC GAAAGCGTTT GGACAACACT CGGGCAGATG AAGCTAATGT TCCAGCAATC 
ACAGAGGGAG GCAACAAGTC CAAAAAAGAG GGCCGTGCCC TTGGGGACTT CAACTCCCTC 
ATCTCCAGTC CGAGGTTAGG TGGGAGAGAT AAAAAGAAAC CTAAAGGTCA AAAAGAGCAG 
CTGAAAGCCA AAAAGCTAAG CAAGGCCACC ACAAATGAGT TCCAGGACAG CTCAGAGAGC 
GAGCCTGAGC TCTTTATCAG TGGAGATGAG CTGATGAACC AGAGCCAGAG TGGGAAGAAG 
AGTTGGAAAG CCAAAAGGAA CCTGCGGGCA GGAGGTTGTG AGCTGGATGA GATCAAATGT 
CGAAAAGCGA ATGAGAAAGA GGACCGAGGC CTCGGCAGCC AGGGCTTCGT CTACATCATG 
GCCAACAAAC AGCCTCTCTG GAATGAAGCC ACGCAGGTCT ACCAGCTAGA CTTTGGAGGG 
AGAGTCACAC AGGAATCAGC AAAGAACTTC CAGATTGAGC TTGAGGGGAG ACAGGTGATG 
CAGTTCGGAA GGATCGACGG GAACGCATAT ATCCTGGACT TTCAGTACCC CTTCTCAGCT 
GTGCAAGCTT TTGCTGTAGC ACTTGCTAAT GTCACCCAGC GCCTCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005168569.1
CDS	701..5911
Translation	MFAAVEHGPVLCSDSNILCLSWKGRVPRSQKEKPVCRRRYCEEGWLATGNGRGVVGVTFTSSHCRRDRNTPQRINFNLRGHNSEVVLVRWNEPFQKLATCDTDGGIFVWIQYEGRWSVELVNDRGAQVSDFTWSHDGTQALISYRDGFVLVGSVSGQRHWSSEINLESQITCGIWTPDDQQVLFGTADGQVIVMDCHGRMLAHILLHEAEGIASMAWNYPSFLVEDSSESDSDSDDSTPPQVHTLKPLLTVCFTSGDISLMSNYDDLSPLLIRTGLKDVVVQWSSQGDLLAVAGMERLVTSDPTQATRNAIVKFYNVRGEHIYSLETPAQRPISTLCWGHRDSRVFLACGPALYVLRVEHRVSSLQLLCQQAIASAVHEEKDLAKLSMPSRLSTYVSSAFAPTIKPPIPDPNNMRDFVSYPTSGNERLHCTMKRTEDNPEVGGPCYTLYLEYLGGLVPILKGRRISKLRPEFVIMDPKTDGKTDEVYGNSFIPSMIDSCNCSDSSDIELNDDWVGKKSPKISRGSKSPKLPRDLVCPFTNRINIDPRKSPKLSRTTQEISRSPRLPIRKPSIGSPSLTRREFPLDDITQHNYLAQVTSNIWGTKFKIVGLAAFLPTNLGAVIYKTSLLHLQPRQMTIYLPEVRKISVEYINLPMFSPNVFSEDEDDLPVTGASGITDDNPPCTVNIPIAPIHSPAQAMSPTQSIGLVQSLLANQNVQLDVLSNPTSTVAPPGVSDQGQHDTVLTAQYTVPARYSSPGQVIFGGMDVGRLLVAQSQQQQQQSQQHQQLQQHYQQQQLQQQLQQYQLQQQQLLQHQHMQQQQQQHMQQQIQQMQQQQQMQQQLQHQIQQQHLQMQLQLQHQQMQQQLQQQHQMQIPVPTLPSGQPSCPVIQLPQSTIPVAAPTAEHSTERGEHELLLKIKTTRSAPQLAEGDAVVFTAPLEISKMNPPPPYPGTVAAAVAAATAAVSAAASASSTSGAVGSSSSTSSSGEVCLKKGDLTLYPPSAQGAQYPTPLGYERITTFDSSGNVEEVCRPRTRLVCNQNVYTLQGPGSSATLRVSSSSSAVEGKKVQLPYASATLNRLTVPRYSIPSGDPPPYPDTANQGGAGRNPGQRLDSSLIHATLRRNSREAALKVSQMLDHQRTLPPKAKASTLSASYQPRGPTALYTCSQCSSNSSGTTSSGSNGIAGGTIIRQDFPPTGGAAPHSTVIVHSNSASPLPSQSSYSLLSLDGTGGVATGAGNRERADYINSAFTEDEALNQSLRQMALNTEVVGLTVKRPPPYQWDPSVTEEIWVPQERTATLPPSGPPAPHKPPPLILGPAQHLDVSRLPFVLSPKSPTSPNNTTFPLAGAYQIALPYPSSAAYSSPPLQAVQASPRPPSPKEAVAPLSFPQQDQSVVLPPGYPPNLPNLACCPLPPMYPGTASCSSLPVTAIPLHPWGSYNPCPPMPSPPGPAPSLPPKASHMLDKPVRSPPPQPPPLPSPPPDLQTAVSPPEAIAEAGEGFQEVLSLNESPVPQRADRFGKKNRKRLDNTRADEANVPAITEGGNKSKKEGRALGDFNSLISSPRLGGRDKKKPKGQKEQLKAKKLSKATTNEFQDSSESEPELFISGDELMNQSQSGKKSWKAKRNLRAGGCELDEIKCRKANEKEDRGLGSQGFVYIMANKQPLWNEATQVYQLDFGGRVTQESAKNFQIELEGRQVMQFGRIDGNAYILDFQYPFSAVQAFAVALANVTQRLK

Target ORF information:

RefSeq Version	XM_005168512.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tubby like protein 4a (tulp4a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005168512.4

ORF Insert Sequence:

ATGTTTGCGG CGGTGGAGCA CGGGCCCGTC CTGTGCAGCG ACTCCAACAT CCTGTGCCTG 
TCCTGGAAGG GCCGCGTGCC GCGCAGCCAG AAGGAGAAGC CGGTGTGTCG GCGACGGTAC 
TGCGAGGAGG GCTGGCTGGC CACGGGGAAC GGCAGAGGGG TGGTGGGAGT CACCTTCACC 
TCCAGCCACT GCAGGAGGGA CCGAAACACT CCACAGCGCA TCAACTTCAA CCTCAGGGGA 
CACAACAGTG AGGTGGTGCT GGTGCGCTGG AACGAGCCCT TCCAGAAGCT GGCGACCTGC 
GATACTGACG GTGGGATCTT CGTCTGGATT CAGTATGAGG GGCGCTGGTC GGTGGAGCTG 
GTCAATGATC GCGGAGCTCA GGTGAGCGAC TTCACCTGGT CTCATGATGG GACGCAGGCT 
CTGATCTCGT ACCGTGATGG TTTCGTGTTG GTGGGTTCGG TGAGCGGTCA GCGCCACTGG 
TCCTCCGAGA TCAACCTTGA GAGTCAGATC ACCTGCGGCA TCTGGACGCC CGACGACCAG 
CAGGTGTTGT TTGGCACTGC AGACGGGCAG GTGATCGTGA TGGACTGCCA CGGCCGCATG 
TTGGCTCATA TTCTGCTCCA TGAGGCCGAG GGCATCGCCA GCATGGCCTG GAACTATCCC 
AGCTTCCTGG TGGAGGACAG CAGCGAGAGC GACTCCGATT CAGACGACAG CACGCCTCCG 
CAGGTGCACA CCCTGAAGCC GCTGCTGACC GTCTGCTTCA CGTCCGGAGA CATCAGTTTG 
ATGAGCAATT ATGATGACCT TTCACCTCTG CTGATACGCA CGGGCCTCAA AGATGTGGTG 
GTGCAGTGGT CTTCCCAGGG GGATCTGCTG GCTGTCGCAG GGATGGAGAG ACTCGTGACT 
TCCGACCCCA CACAGGCCAC CCGAAACGCC ATTGTAAAGT TTTACAATGT TCGCGGGGAG 
CACATTTACT CACTGGAAAC ACCTGCTCAG AGGCCGATCA GTACGCTGTG CTGGGGCCAC 
CGGGACTCGC GTGTGTTTCT GGCCTGCGGG CCGGCGCTGT ATGTGCTGCG GGTCGAGCAC 
AGAGTCTCCA GCCTGCAGCT TCTGTGCCAA CAGGCCATCG CCAGTGCCGT ACACGAGGAG 
AAAGATCTGG CCAAGCTCAG CATGCCCTCC CGCCTCAGCA CATATGTGTC TTCTGCCTTC 
GCTCCCACTA TTAAGCCTCC AATCCCAGAT CCCAACAACA TGCGGGACTT TGTTAGCTAT 
CCGACGTCAG GTAATGAGCG TCTGCACTGC ACCATGAAGC GAACGGAGGA CAACCCTGAG 
GTCGGCGGTC CATGCTACAC CTTATACCTG GAGTATCTGG GAGGACTCGT TCCTATTCTG 
AAAGGCCGTA GGATAAGCAA ACTTCGTCCT GAATTTGTCA TCATGGACCC GAAGACTGAT 
GGGAAAACAG ATGAAGTGTA TGGGAACAGC TTCATCCCCT CCATGATTGA CAGCTGCAAT 
TGCTCAGATT CAAGTGACAT TGAGCTCAAT GATGATTGGG TAGGAAAGAA ATCACCAAAG 
ATCTCCAGAG GGAGCAAGTC TCCCAAACTT CCCAGAGACT TAGTGTGTCC CTTTACCAAC 
AGGATAAATA TAGATCCTCG CAAGTCCCCC AAGCTGTCCC GCACCACACA GGAAATATCC 
CGATCGCCAC GGTTACCCAT TCGCAAGCCC TCTATTGGCT CTCCAAGTCT GACTCGCAGA 
GAGTTCCCAC TTGATGACAT CACACAGCAC AATTACCTTG CGCAAGTTAC ATCTAACATA 
TGGGGAACAA AGTTCAAAAT TGTGGGACTT GCAGCATTTT TGCCAACAAA TCTTGGTGCA 
GTTATCTACA AGACAAGTCT GCTTCACCTC CAGCCCAGAC AGATGACCAT CTACCTCCCT 
GAGGTGAGGA AGATCTCGGT GGAATACATT AACCTTCCTA TGTTCAGTCC TAATGTCTTC 
AGTGAGGACG AGGACGACCT GCCTGTCACA GGAGCTTCAG GAATCACTGA TGATAATCCA 
CCTTGCACTG TTAACATACC CATTGCCCCC ATACACAGCC CTGCCCAAGC CATGTCCCCA 
ACTCAAAGCA TTGGCCTAGT CCAATCCCTA CTAGCCAATC AGAATGTCCA GTTAGACGTA 
TTGTCCAACC CAACATCGAC TGTGGCTCCT CCTGGGGTGT CTGATCAAGG ACAGCATGAC 
ACTGTGCTTA CGGCACAATA CACTGTTCCT GCCAGGTATT CTAGTCCTGG TCAGGTAATC 
TTCGGAGGTA TGGATGTTGG TCGGCTTTTA GTTGCACAGT CCCAACAGCA GCAGCAGCAA 
TCACAACAAC ATCAGCAGCT GCAACAGCAT TATCAGCAAC AGCAGCTACA GCAACAGCTG 
CAACAATATC AGCTGCAGCA GCAACAGCTG TTGCAGCATC AGCACATGCA GCAACAGCAG 
CAGCAACACA TGCAGCAGCA GATCCAACAG ATGCAGCAAC AGCAGCAGAT GCAGCAACAG 
CTTCAGCATC AAATACAGCA GCAGCATCTG CAAATGCAAC TGCAGCTACA GCACCAGCAA 
ATGCAGCAGC AGTTGCAACA GCAGCATCAG ATGCAAATCC CTGTTCCAAC TTTGCCCTCA 
GGTCAACCAT CATGCCCAGT CATTCAGCTT CCACAAAGTA CAATACCAGT CGCTGCCCCT 
ACTGCAGAGC ACAGCACAGA GCGCGGGGAG CATGAGCTCC TGCTTAAAAT TAAAACCACT 
CGTTCTGCCC CACAACTAGC TGAAGGTGAT GCAGTGGTTT TTACAGCACC TCTTGAGATT 
AGCAAAATGA ACCCGCCCCC TCCTTACCCT GGCACAGTCG CCGCTGCAGT GGCAGCTGCT 
ACTGCTGCAG TTTCGGCTGC TGCTTCTGCA AGTTCCACCT CTGGGGCAGT GGGAAGCAGC 
TCTAGCACGT CATCCTCAGG GGAAGTTTGT CTGAAGAAAG GGGATCTGAC CTTGTATCCA 
CCTAGTGCAC AAGGGGCTCA GTACCCAACT CCATTGGGCT ATGAACGCAT CACCACATTT 
GACAGCAGTG GAAATGTGGA AGAGGTGTGC CGACCCCGCA CACGACTTGT TTGCAACCAG 
AATGTTTACA CGCTGCAGGG CCCAGGAAGC TCTGCCACCC TCAGGGTCTC ATCATCTTCC 
TCTGCTGTAG AGGGGAAGAA GGTGCAGCTG CCCTATGCCT CTGCTACGTT GAACCGTCTC 
ACTGTGCCAA GGTACTCTAT ACCCAGTGGG GACCCTCCTC CATACCCTGA TACAGCTAAC 
CAGGGCGGAG CAGGGCGCAA CCCTGGCCAG AGACTGGACA GCAGCTTGAT TCATGCTACC 
TTGAGGCGCA ACAGTCGGGA AGCTGCATTG AAGGTTTCCC AAATGCTGGA TCACCAGCGG 
ACTTTACCCC CCAAGGCTAA AGCCAGTACT TTGTCTGCTT CCTATCAACC AAGAGGGCCG 
ACAGCTCTTT ATACATGCAG TCAGTGCAGT AGCAACAGTA GTGGAACCAC AAGCAGTGGA 
AGTAACGGAA TAGCTGGGGG CACAATCATT AGGCAAGATT TCCCACCAAC TGGGGGTGCT 
GCACCTCATA GCACTGTTAT AGTGCACTCC AACAGTGCCT CGCCTCTGCC TTCTCAATCC 
TCCTACAGTC TGCTCAGTCT GGATGGCACC GGTGGAGTGG CTACTGGAGC TGGAAACAGG 
GAAAGAGCTG ACTATATCAA CTCAGCTTTT ACAGAGGACG AGGCATTGAA TCAGTCTCTG 
AGGCAAATGG CCCTTAATAC CGAGGTAGTG GGTTTGACAG TCAAACGGCC TCCACCGTAT 
CAATGGGACC CATCTGTTAC AGAAGAGATT TGGGTTCCTC AAGAGCGCAC AGCGACTCTG 
CCCCCTTCTG GACCTCCTGC ACCTCACAAA CCTCCTCCGC TCATCCTCGG TCCTGCACAG 
CATCTGGACG TTTCTCGTCT TCCGTTTGTC CTCTCACCTA AATCTCCAAC AAGTCCTAAC 
AACACCACTT TTCCACTAGC AGGAGCTTAT CAGATTGCAT TGCCCTATCC ATCAAGTGCT 
GCATACAGTA GTCCCCCACT GCAGGCAGTG CAAGCATCTC CACGGCCGCC TTCTCCTAAA 
GAGGCGGTTG CTCCACTTTC CTTTCCCCAG CAAGACCAGT CAGTGGTCTT GCCTCCTGGT 
TATCCACCAA ATTTGCCCAA CCTGGCTTGC TGTCCCCTCC CACCAATGTA CCCTGGCACA 
GCTTCTTGTT CTAGCCTGCC TGTAACCGCC ATTCCTCTTC ATCCATGGGG TTCTTACAAT 
CCCTGTCCCC CCATGCCAAG CCCCCCGGGT CCCGCCCCAT CTCTCCCACC CAAGGCCTCC 
CACATGCTGG ATAAACCTGT TCGTTCTCCA CCTCCTCAAC CGCCGCCTTT GCCTTCGCCT 
CCCCCAGACC TCCAGACTGC GGTCAGTCCC CCAGAAGCAA TAGCTGAAGC CGGGGAAGGC 
TTTCAAGAGG TGCTCTCGCT TAACGAAAGC CCTGTTCCTC AACGAGCAGA CAGGTTTGGC 
AAGAAGAATC GAAAGCGTTT GGACAACACT CGGGCAGATG AAGCTAATGT TCCAGCAATC 
ACAGAGGGAG GCAACAAGTC CAAAAAAGAG GGCCGTGCCC TTGGGGACTT CAACTCCCTC 
ATCTCCAGTC CGAGGTTAGG TGGGAGAGAT AAAAAGAAAC CTAAAGGTCA AAAAGAGCAG 
CTGAAAGCCA AAAAGCTAAG CAAGGCCACC ACAAATGAGT TCCAGGACAG CTCAGAGAGC 
GAGCCTGAGC TCTTTATCAG TGGAGATGAG CTGATGAACC AGAGCCAGAG TGGGAAGAAG 
AGTTGGAAAG CCAAAAGGAA CCTGCGGGCA GGAGGTTGTG AGCTGGATGA GATCAAATGT 
CGAAAAGCGA ATGAGAAAGA GGACCGAGGC CTCGGCAGCC AGGGCTTCGT CTACATCATG 
GCCAACAAAC AGCCTCTCTG GAATGAAGCC ACGCAGGTCT ACCAGCTAGA CTTTGGAGGG 
AGAGTCACAC AGGAATCAGC AAAGAACTTC CAGATTGAGC TTGAGGGGAG ACAGGTGATG 
CAGTTCGGAA GGATCGACGG GAACGCATAT ATCCTGGACT TTCAGTACCC CTTCTCAGCT 
GTGCAAGCTT TTGCTGTAGC ACTTGCTAAT GTCACCCAGC GCCTCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tulp4a ( XM_005168512.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005168569.1 Danio rerio tubby like protein 4a (tulp4a), transcript variant X1, mRNA.

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CloneID	ODa08999
Clone ID Related Accession (Same CDS sequence)	XM_005168512.4 , XM_005168512.3
Accession Version	XM_005168512.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	tubby-related protein 4 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005168512.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGCGG CGGTGGAGCA CGGGCCCGTC CTGTGCAGCG ACTCCAACAT CCTGTGCCTG 
TCCTGGAAGG GCCGCGTGCC GCGCAGCCAG AAGGAGAAGC CGGTGTGTCG GCGACGGTAC 
TGCGAGGAGG GCTGGCTGGC CACGGGGAAC GGCAGAGGGG TGGTGGGAGT CACCTTCACC 
TCCAGCCACT GCAGGAGGGA CCGAAACACT CCACAGCGCA TCAACTTCAA CCTCAGGGGA 
CACAACAGTG AGGTGGTGCT GGTGCGCTGG AACGAGCCCT TCCAGAAGCT GGCGACCTGC 
GATACTGACG GTGGGATCTT CGTCTGGATT CAGTATGAGG GGCGCTGGTC GGTGGAGCTG 
GTCAATGATC GCGGAGCTCA GGTGAGCGAC TTCACCTGGT CTCATGATGG GACGCAGGCT 
CTGATCTCGT ACCGTGATGG TTTCGTGTTG GTGGGTTCGG TGAGCGGTCA GCGCCACTGG 
TCCTCCGAGA TCAACCTTGA GAGTCAGATC ACCTGCGGCA TCTGGACGCC CGACGACCAG 
CAGGTGTTGT TTGGCACTGC AGACGGGCAG GTGATCGTGA TGGACTGCCA CGGCCGCATG 
TTGGCTCATA TTCTGCTCCA TGAGGCCGAG GGCATCGCCA GCATGGCCTG GAACTATCCC 
AGCTTCCTGG TGGAGGACAG CAGCGAGAGC GACTCCGATT CAGACGACAG CACGCCTCCG 
CAGGTGCACA CCCTGAAGCC GCTGCTGACC GTCTGCTTCA CGTCCGGAGA CATCAGTTTG 
ATGAGCAATT ATGATGACCT TTCACCTCTG CTGATACGCA CGGGCCTCAA AGATGTGGTG 
GTGCAGTGGT CTTCCCAGGG GGATCTGCTG GCTGTCGCAG GGATGGAGAG ACTCGTGACT 
TCCGACCCCA CACAGGCCAC CCGAAACGCC ATTGTAAAGT TTTACAATGT TCGCGGGGAG 
CACATTTACT CACTGGAAAC ACCTGCTCAG AGGCCGATCA GTACGCTGTG CTGGGGCCAC 
CGGGACTCGC GTGTGTTTCT GGCCTGCGGG CCGGCGCTGT ATGTGCTGCG GGTCGAGCAC 
AGAGTCTCCA GCCTGCAGCT TCTGTGCCAA CAGGCCATCG CCAGTGCCGT ACACGAGGAG 
AAAGATCTGG CCAAGCTCAG CATGCCCTCC CGCCTCAGCA CATATGTGTC TTCTGCCTTC 
GCTCCCACTA TTAAGCCTCC AATCCCAGAT CCCAACAACA TGCGGGACTT TGTTAGCTAT 
CCGACGTCAG GTAATGAGCG TCTGCACTGC ACCATGAAGC GAACGGAGGA CAACCCTGAG 
GTCGGCGGTC CATGCTACAC CTTATACCTG GAGTATCTGG GAGGACTCGT TCCTATTCTG 
AAAGGCCGTA GGATAAGCAA ACTTCGTCCT GAATTTGTCA TCATGGACCC GAAGACTGAT 
GGGAAAACAG ATGAAGTGTA TGGGAACAGC TTCATCCCCT CCATGATTGA CAGCTGCAAT 
TGCTCAGATT CAAGTGACAT TGAGCTCAAT GATGATTGGG TAGGAAAGAA ATCACCAAAG 
ATCTCCAGAG GGAGCAAGTC TCCCAAACTT CCCAGAGACT TAGTGTGTCC CTTTACCAAC 
AGGATAAATA TAGATCCTCG CAAGTCCCCC AAGCTGTCCC GCACCACACA GGAAATATCC 
CGATCGCCAC GGTTACCCAT TCGCAAGCCC TCTATTGGCT CTCCAAGTCT GACTCGCAGA 
GAGTTCCCAC TTGATGACAT CACACAGCAC AATTACCTTG CGCAAGTTAC ATCTAACATA 
TGGGGAACAA AGTTCAAAAT TGTGGGACTT GCAGCATTTT TGCCAACAAA TCTTGGTGCA 
GTTATCTACA AGACAAGTCT GCTTCACCTC CAGCCCAGAC AGATGACCAT CTACCTCCCT 
GAGGTGAGGA AGATCTCGGT GGAATACATT AACCTTCCTA TGTTCAGTCC TAATGTCTTC 
AGTGAGGACG AGGACGACCT GCCTGTCACA GGAGCTTCAG GAATCACTGA TGATAATCCA 
CCTTGCACTG TTAACATACC CATTGCCCCC ATACACAGCC CTGCCCAAGC CATGTCCCCA 
ACTCAAAGCA TTGGCCTAGT CCAATCCCTA CTAGCCAATC AGAATGTCCA GTTAGACGTA 
TTGTCCAACC CAACATCGAC TGTGGCTCCT CCTGGGGTGT CTGATCAAGG ACAGCATGAC 
ACTGTGCTTA CGGCACAATA CACTGTTCCT GCCAGGTATT CTAGTCCTGG TCAGGTAATC 
TTCGGAGGTA TGGATGTTGG TCGGCTTTTA GTTGCACAGT CCCAACAGCA GCAGCAGCAA 
TCACAACAAC ATCAGCAGCT GCAACAGCAT TATCAGCAAC AGCAGCTACA GCAACAGCTG 
CAACAATATC AGCTGCAGCA GCAACAGCTG TTGCAGCATC AGCACATGCA GCAACAGCAG 
CAGCAACACA TGCAGCAGCA GATCCAACAG ATGCAGCAAC AGCAGCAGAT GCAGCAACAG 
CTTCAGCATC AAATACAGCA GCAGCATCTG CAAATGCAAC TGCAGCTACA GCACCAGCAA 
ATGCAGCAGC AGTTGCAACA GCAGCATCAG ATGCAAATCC CTGTTCCAAC TTTGCCCTCA 
GGTCAACCAT CATGCCCAGT CATTCAGCTT CCACAAAGTA CAATACCAGT CGCTGCCCCT 
ACTGCAGAGC ACAGCACAGA GCGCGGGGAG CATGAGCTCC TGCTTAAAAT TAAAACCACT 
CGTTCTGCCC CACAACTAGC TGAAGGTGAT GCAGTGGTTT TTACAGCACC TCTTGAGATT 
AGCAAAATGA ACCCGCCCCC TCCTTACCCT GGCACAGTCG CCGCTGCAGT GGCAGCTGCT 
ACTGCTGCAG TTTCGGCTGC TGCTTCTGCA AGTTCCACCT CTGGGGCAGT GGGAAGCAGC 
TCTAGCACGT CATCCTCAGG GGAAGTTTGT CTGAAGAAAG GGGATCTGAC CTTGTATCCA 
CCTAGTGCAC AAGGGGCTCA GTACCCAACT CCATTGGGCT ATGAACGCAT CACCACATTT 
GACAGCAGTG GAAATGTGGA AGAGGTGTGC CGACCCCGCA CACGACTTGT TTGCAACCAG 
AATGTTTACA CGCTGCAGGG CCCAGGAAGC TCTGCCACCC TCAGGGTCTC ATCATCTTCC 
TCTGCTGTAG AGGGGAAGAA GGTGCAGCTG CCCTATGCCT CTGCTACGTT GAACCGTCTC 
ACTGTGCCAA GGTACTCTAT ACCCAGTGGG GACCCTCCTC CATACCCTGA TACAGCTAAC 
CAGGGCGGAG CAGGGCGCAA CCCTGGCCAG AGACTGGACA GCAGCTTGAT TCATGCTACC 
TTGAGGCGCA ACAGTCGGGA AGCTGCATTG AAGGTTTCCC AAATGCTGGA TCACCAGCGG 
ACTTTACCCC CCAAGGCTAA AGCCAGTACT TTGTCTGCTT CCTATCAACC AAGAGGGCCG 
ACAGCTCTTT ATACATGCAG TCAGTGCAGT AGCAACAGTA GTGGAACCAC AAGCAGTGGA 
AGTAACGGAA TAGCTGGGGG CACAATCATT AGGCAAGATT TCCCACCAAC TGGGGGTGCT 
GCACCTCATA GCACTGTTAT AGTGCACTCC AACAGTGCCT CGCCTCTGCC TTCTCAATCC 
TCCTACAGTC TGCTCAGTCT GGATGGCACC GGTGGAGTGG CTACTGGAGC TGGAAACAGG 
GAAAGAGCTG ACTATATCAA CTCAGCTTTT ACAGAGGACG AGGCATTGAA TCAGTCTCTG 
AGGCAAATGG CCCTTAATAC CGAGGTAGTG GGTTTGACAG TCAAACGGCC TCCACCGTAT 
CAATGGGACC CATCTGTTAC AGAAGAGATT TGGGTTCCTC AAGAGCGCAC AGCGACTCTG 
CCCCCTTCTG GACCTCCTGC ACCTCACAAA CCTCCTCCGC TCATCCTCGG TCCTGCACAG 
CATCTGGACG TTTCTCGTCT TCCGTTTGTC CTCTCACCTA AATCTCCAAC AAGTCCTAAC 
AACACCACTT TTCCACTAGC AGGAGCTTAT CAGATTGCAT TGCCCTATCC ATCAAGTGCT 
GCATACAGTA GTCCCCCACT GCAGGCAGTG CAAGCATCTC CACGGCCGCC TTCTCCTAAA 
GAGGCGGTTG CTCCACTTTC CTTTCCCCAG CAAGACCAGT CAGTGGTCTT GCCTCCTGGT 
TATCCACCAA ATTTGCCCAA CCTGGCTTGC TGTCCCCTCC CACCAATGTA CCCTGGCACA 
GCTTCTTGTT CTAGCCTGCC TGTAACCGCC ATTCCTCTTC ATCCATGGGG TTCTTACAAT 
CCCTGTCCCC CCATGCCAAG CCCCCCGGGT CCCGCCCCAT CTCTCCCACC CAAGGCCTCC 
CACATGCTGG ATAAACCTGT TCGTTCTCCA CCTCCTCAAC CGCCGCCTTT GCCTTCGCCT 
CCCCCAGACC TCCAGACTGC GGTCAGTCCC CCAGAAGCAA TAGCTGAAGC CGGGGAAGGC 
TTTCAAGAGG TGCTCTCGCT TAACGAAAGC CCTGTTCCTC AACGAGCAGA CAGGTTTGGC 
AAGAAGAATC GAAAGCGTTT GGACAACACT CGGGCAGATG AAGCTAATGT TCCAGCAATC 
ACAGAGGGAG GCAACAAGTC CAAAAAAGAG GGCCGTGCCC TTGGGGACTT CAACTCCCTC 
ATCTCCAGTC CGAGGTTAGG TGGGAGAGAT AAAAAGAAAC CTAAAGGTCA AAAAGAGCAG 
CTGAAAGCCA AAAAGCTAAG CAAGGCCACC ACAAATGAGT TCCAGGACAG CTCAGAGAGC 
GAGCCTGAGC TCTTTATCAG TGGAGATGAG CTGATGAACC AGAGCCAGAG TGGGAAGAAG 
AGTTGGAAAG CCAAAAGGAA CCTGCGGGCA GGAGGTTGTG AGCTGGATGA GATCAAATGT 
CGAAAAGCGA ATGAGAAAGA GGACCGAGGC CTCGGCAGCC AGGGCTTCGT CTACATCATG 
GCCAACAAAC AGCCTCTCTG GAATGAAGCC ACGCAGGTCT ACCAGCTAGA CTTTGGAGGG 
AGAGTCACAC AGGAATCAGC AAAGAACTTC CAGATTGAGC TTGAGGGGAG ACAGGTGATG 
CAGTTCGGAA GGATCGACGG GAACGCATAT ATCCTGGACT TTCAGTACCC CTTCTCAGCT 
GTGCAAGCTT TTGCTGTAGC ACTTGCTAAT GTCACCCAGC GCCTCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005168569.1
CDS	647..5857
Translation	MFAAVEHGPVLCSDSNILCLSWKGRVPRSQKEKPVCRRRYCEEGWLATGNGRGVVGVTFTSSHCRRDRNTPQRINFNLRGHNSEVVLVRWNEPFQKLATCDTDGGIFVWIQYEGRWSVELVNDRGAQVSDFTWSHDGTQALISYRDGFVLVGSVSGQRHWSSEINLESQITCGIWTPDDQQVLFGTADGQVIVMDCHGRMLAHILLHEAEGIASMAWNYPSFLVEDSSESDSDSDDSTPPQVHTLKPLLTVCFTSGDISLMSNYDDLSPLLIRTGLKDVVVQWSSQGDLLAVAGMERLVTSDPTQATRNAIVKFYNVRGEHIYSLETPAQRPISTLCWGHRDSRVFLACGPALYVLRVEHRVSSLQLLCQQAIASAVHEEKDLAKLSMPSRLSTYVSSAFAPTIKPPIPDPNNMRDFVSYPTSGNERLHCTMKRTEDNPEVGGPCYTLYLEYLGGLVPILKGRRISKLRPEFVIMDPKTDGKTDEVYGNSFIPSMIDSCNCSDSSDIELNDDWVGKKSPKISRGSKSPKLPRDLVCPFTNRINIDPRKSPKLSRTTQEISRSPRLPIRKPSIGSPSLTRREFPLDDITQHNYLAQVTSNIWGTKFKIVGLAAFLPTNLGAVIYKTSLLHLQPRQMTIYLPEVRKISVEYINLPMFSPNVFSEDEDDLPVTGASGITDDNPPCTVNIPIAPIHSPAQAMSPTQSIGLVQSLLANQNVQLDVLSNPTSTVAPPGVSDQGQHDTVLTAQYTVPARYSSPGQVIFGGMDVGRLLVAQSQQQQQQSQQHQQLQQHYQQQQLQQQLQQYQLQQQQLLQHQHMQQQQQQHMQQQIQQMQQQQQMQQQLQHQIQQQHLQMQLQLQHQQMQQQLQQQHQMQIPVPTLPSGQPSCPVIQLPQSTIPVAAPTAEHSTERGEHELLLKIKTTRSAPQLAEGDAVVFTAPLEISKMNPPPPYPGTVAAAVAAATAAVSAAASASSTSGAVGSSSSTSSSGEVCLKKGDLTLYPPSAQGAQYPTPLGYERITTFDSSGNVEEVCRPRTRLVCNQNVYTLQGPGSSATLRVSSSSSAVEGKKVQLPYASATLNRLTVPRYSIPSGDPPPYPDTANQGGAGRNPGQRLDSSLIHATLRRNSREAALKVSQMLDHQRTLPPKAKASTLSASYQPRGPTALYTCSQCSSNSSGTTSSGSNGIAGGTIIRQDFPPTGGAAPHSTVIVHSNSASPLPSQSSYSLLSLDGTGGVATGAGNRERADYINSAFTEDEALNQSLRQMALNTEVVGLTVKRPPPYQWDPSVTEEIWVPQERTATLPPSGPPAPHKPPPLILGPAQHLDVSRLPFVLSPKSPTSPNNTTFPLAGAYQIALPYPSSAAYSSPPLQAVQASPRPPSPKEAVAPLSFPQQDQSVVLPPGYPPNLPNLACCPLPPMYPGTASCSSLPVTAIPLHPWGSYNPCPPMPSPPGPAPSLPPKASHMLDKPVRSPPPQPPPLPSPPPDLQTAVSPPEAIAEAGEGFQEVLSLNESPVPQRADRFGKKNRKRLDNTRADEANVPAITEGGNKSKKEGRALGDFNSLISSPRLGGRDKKKPKGQKEQLKAKKLSKATTNEFQDSSESEPELFISGDELMNQSQSGKKSWKAKRNLRAGGCELDEIKCRKANEKEDRGLGSQGFVYIMANKQPLWNEATQVYQLDFGGRVTQESAKNFQIELEGRQVMQFGRIDGNAYILDFQYPFSAVQAFAVALANVTQRLK

Target ORF information:

RefSeq Version	XM_005168512.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tubby like protein 4a (tulp4a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005168512.3

ORF Insert Sequence:

ATGTTTGCGG CGGTGGAGCA CGGGCCCGTC CTGTGCAGCG ACTCCAACAT CCTGTGCCTG 
TCCTGGAAGG GCCGCGTGCC GCGCAGCCAG AAGGAGAAGC CGGTGTGTCG GCGACGGTAC 
TGCGAGGAGG GCTGGCTGGC CACGGGGAAC GGCAGAGGGG TGGTGGGAGT CACCTTCACC 
TCCAGCCACT GCAGGAGGGA CCGAAACACT CCACAGCGCA TCAACTTCAA CCTCAGGGGA 
CACAACAGTG AGGTGGTGCT GGTGCGCTGG AACGAGCCCT TCCAGAAGCT GGCGACCTGC 
GATACTGACG GTGGGATCTT CGTCTGGATT CAGTATGAGG GGCGCTGGTC GGTGGAGCTG 
GTCAATGATC GCGGAGCTCA GGTGAGCGAC TTCACCTGGT CTCATGATGG GACGCAGGCT 
CTGATCTCGT ACCGTGATGG TTTCGTGTTG GTGGGTTCGG TGAGCGGTCA GCGCCACTGG 
TCCTCCGAGA TCAACCTTGA GAGTCAGATC ACCTGCGGCA TCTGGACGCC CGACGACCAG 
CAGGTGTTGT TTGGCACTGC AGACGGGCAG GTGATCGTGA TGGACTGCCA CGGCCGCATG 
TTGGCTCATA TTCTGCTCCA TGAGGCCGAG GGCATCGCCA GCATGGCCTG GAACTATCCC 
AGCTTCCTGG TGGAGGACAG CAGCGAGAGC GACTCCGATT CAGACGACAG CACGCCTCCG 
CAGGTGCACA CCCTGAAGCC GCTGCTGACC GTCTGCTTCA CGTCCGGAGA CATCAGTTTG 
ATGAGCAATT ATGATGACCT TTCACCTCTG CTGATACGCA CGGGCCTCAA AGATGTGGTG 
GTGCAGTGGT CTTCCCAGGG GGATCTGCTG GCTGTCGCAG GGATGGAGAG ACTCGTGACT 
TCCGACCCCA CACAGGCCAC CCGAAACGCC ATTGTAAAGT TTTACAATGT TCGCGGGGAG 
CACATTTACT CACTGGAAAC ACCTGCTCAG AGGCCGATCA GTACGCTGTG CTGGGGCCAC 
CGGGACTCGC GTGTGTTTCT GGCCTGCGGG CCGGCGCTGT ATGTGCTGCG GGTCGAGCAC 
AGAGTCTCCA GCCTGCAGCT TCTGTGCCAA CAGGCCATCG CCAGTGCCGT ACACGAGGAG 
AAAGATCTGG CCAAGCTCAG CATGCCCTCC CGCCTCAGCA CATATGTGTC TTCTGCCTTC 
GCTCCCACTA TTAAGCCTCC AATCCCAGAT CCCAACAACA TGCGGGACTT TGTTAGCTAT 
CCGACGTCAG GTAATGAGCG TCTGCACTGC ACCATGAAGC GAACGGAGGA CAACCCTGAG 
GTCGGCGGTC CATGCTACAC CTTATACCTG GAGTATCTGG GAGGACTCGT TCCTATTCTG 
AAAGGCCGTA GGATAAGCAA ACTTCGTCCT GAATTTGTCA TCATGGACCC GAAGACTGAT 
GGGAAAACAG ATGAAGTGTA TGGGAACAGC TTCATCCCCT CCATGATTGA CAGCTGCAAT 
TGCTCAGATT CAAGTGACAT TGAGCTCAAT GATGATTGGG TAGGAAAGAA ATCACCAAAG 
ATCTCCAGAG GGAGCAAGTC TCCCAAACTT CCCAGAGACT TAGTGTGTCC CTTTACCAAC 
AGGATAAATA TAGATCCTCG CAAGTCCCCC AAGCTGTCCC GCACCACACA GGAAATATCC 
CGATCGCCAC GGTTACCCAT TCGCAAGCCC TCTATTGGCT CTCCAAGTCT GACTCGCAGA 
GAGTTCCCAC TTGATGACAT CACACAGCAC AATTACCTTG CGCAAGTTAC ATCTAACATA 
TGGGGAACAA AGTTCAAAAT TGTGGGACTT GCAGCATTTT TGCCAACAAA TCTTGGTGCA 
GTTATCTACA AGACAAGTCT GCTTCACCTC CAGCCCAGAC AGATGACCAT CTACCTCCCT 
GAGGTGAGGA AGATCTCGGT GGAATACATT AACCTTCCTA TGTTCAGTCC TAATGTCTTC 
AGTGAGGACG AGGACGACCT GCCTGTCACA GGAGCTTCAG GAATCACTGA TGATAATCCA 
CCTTGCACTG TTAACATACC CATTGCCCCC ATACACAGCC CTGCCCAAGC CATGTCCCCA 
ACTCAAAGCA TTGGCCTAGT CCAATCCCTA CTAGCCAATC AGAATGTCCA GTTAGACGTA 
TTGTCCAACC CAACATCGAC TGTGGCTCCT CCTGGGGTGT CTGATCAAGG ACAGCATGAC 
ACTGTGCTTA CGGCACAATA CACTGTTCCT GCCAGGTATT CTAGTCCTGG TCAGGTAATC 
TTCGGAGGTA TGGATGTTGG TCGGCTTTTA GTTGCACAGT CCCAACAGCA GCAGCAGCAA 
TCACAACAAC ATCAGCAGCT GCAACAGCAT TATCAGCAAC AGCAGCTACA GCAACAGCTG 
CAACAATATC AGCTGCAGCA GCAACAGCTG TTGCAGCATC AGCACATGCA GCAACAGCAG 
CAGCAACACA TGCAGCAGCA GATCCAACAG ATGCAGCAAC AGCAGCAGAT GCAGCAACAG 
CTTCAGCATC AAATACAGCA GCAGCATCTG CAAATGCAAC TGCAGCTACA GCACCAGCAA 
ATGCAGCAGC AGTTGCAACA GCAGCATCAG ATGCAAATCC CTGTTCCAAC TTTGCCCTCA 
GGTCAACCAT CATGCCCAGT CATTCAGCTT CCACAAAGTA CAATACCAGT CGCTGCCCCT 
ACTGCAGAGC ACAGCACAGA GCGCGGGGAG CATGAGCTCC TGCTTAAAAT TAAAACCACT 
CGTTCTGCCC CACAACTAGC TGAAGGTGAT GCAGTGGTTT TTACAGCACC TCTTGAGATT 
AGCAAAATGA ACCCGCCCCC TCCTTACCCT GGCACAGTCG CCGCTGCAGT GGCAGCTGCT 
ACTGCTGCAG TTTCGGCTGC TGCTTCTGCA AGTTCCACCT CTGGGGCAGT GGGAAGCAGC 
TCTAGCACGT CATCCTCAGG GGAAGTTTGT CTGAAGAAAG GGGATCTGAC CTTGTATCCA 
CCTAGTGCAC AAGGGGCTCA GTACCCAACT CCATTGGGCT ATGAACGCAT CACCACATTT 
GACAGCAGTG GAAATGTGGA AGAGGTGTGC CGACCCCGCA CACGACTTGT TTGCAACCAG 
AATGTTTACA CGCTGCAGGG CCCAGGAAGC TCTGCCACCC TCAGGGTCTC ATCATCTTCC 
TCTGCTGTAG AGGGGAAGAA GGTGCAGCTG CCCTATGCCT CTGCTACGTT GAACCGTCTC 
ACTGTGCCAA GGTACTCTAT ACCCAGTGGG GACCCTCCTC CATACCCTGA TACAGCTAAC 
CAGGGCGGAG CAGGGCGCAA CCCTGGCCAG AGACTGGACA GCAGCTTGAT TCATGCTACC 
TTGAGGCGCA ACAGTCGGGA AGCTGCATTG AAGGTTTCCC AAATGCTGGA TCACCAGCGG 
ACTTTACCCC CCAAGGCTAA AGCCAGTACT TTGTCTGCTT CCTATCAACC AAGAGGGCCG 
ACAGCTCTTT ATACATGCAG TCAGTGCAGT AGCAACAGTA GTGGAACCAC AAGCAGTGGA 
AGTAACGGAA TAGCTGGGGG CACAATCATT AGGCAAGATT TCCCACCAAC TGGGGGTGCT 
GCACCTCATA GCACTGTTAT AGTGCACTCC AACAGTGCCT CGCCTCTGCC TTCTCAATCC 
TCCTACAGTC TGCTCAGTCT GGATGGCACC GGTGGAGTGG CTACTGGAGC TGGAAACAGG 
GAAAGAGCTG ACTATATCAA CTCAGCTTTT ACAGAGGACG AGGCATTGAA TCAGTCTCTG 
AGGCAAATGG CCCTTAATAC CGAGGTAGTG GGTTTGACAG TCAAACGGCC TCCACCGTAT 
CAATGGGACC CATCTGTTAC AGAAGAGATT TGGGTTCCTC AAGAGCGCAC AGCGACTCTG 
CCCCCTTCTG GACCTCCTGC ACCTCACAAA CCTCCTCCGC TCATCCTCGG TCCTGCACAG 
CATCTGGACG TTTCTCGTCT TCCGTTTGTC CTCTCACCTA AATCTCCAAC AAGTCCTAAC 
AACACCACTT TTCCACTAGC AGGAGCTTAT CAGATTGCAT TGCCCTATCC ATCAAGTGCT 
GCATACAGTA GTCCCCCACT GCAGGCAGTG CAAGCATCTC CACGGCCGCC TTCTCCTAAA 
GAGGCGGTTG CTCCACTTTC CTTTCCCCAG CAAGACCAGT CAGTGGTCTT GCCTCCTGGT 
TATCCACCAA ATTTGCCCAA CCTGGCTTGC TGTCCCCTCC CACCAATGTA CCCTGGCACA 
GCTTCTTGTT CTAGCCTGCC TGTAACCGCC ATTCCTCTTC ATCCATGGGG TTCTTACAAT 
CCCTGTCCCC CCATGCCAAG CCCCCCGGGT CCCGCCCCAT CTCTCCCACC CAAGGCCTCC 
CACATGCTGG ATAAACCTGT TCGTTCTCCA CCTCCTCAAC CGCCGCCTTT GCCTTCGCCT 
CCCCCAGACC TCCAGACTGC GGTCAGTCCC CCAGAAGCAA TAGCTGAAGC CGGGGAAGGC 
TTTCAAGAGG TGCTCTCGCT TAACGAAAGC CCTGTTCCTC AACGAGCAGA CAGGTTTGGC 
AAGAAGAATC GAAAGCGTTT GGACAACACT CGGGCAGATG AAGCTAATGT TCCAGCAATC 
ACAGAGGGAG GCAACAAGTC CAAAAAAGAG GGCCGTGCCC TTGGGGACTT CAACTCCCTC 
ATCTCCAGTC CGAGGTTAGG TGGGAGAGAT AAAAAGAAAC CTAAAGGTCA AAAAGAGCAG 
CTGAAAGCCA AAAAGCTAAG CAAGGCCACC ACAAATGAGT TCCAGGACAG CTCAGAGAGC 
GAGCCTGAGC TCTTTATCAG TGGAGATGAG CTGATGAACC AGAGCCAGAG TGGGAAGAAG 
AGTTGGAAAG CCAAAAGGAA CCTGCGGGCA GGAGGTTGTG AGCTGGATGA GATCAAATGT 
CGAAAAGCGA ATGAGAAAGA GGACCGAGGC CTCGGCAGCC AGGGCTTCGT CTACATCATG 
GCCAACAAAC AGCCTCTCTG GAATGAAGCC ACGCAGGTCT ACCAGCTAGA CTTTGGAGGG 
AGAGTCACAC AGGAATCAGC AAAGAACTTC CAGATTGAGC TTGAGGGGAG ACAGGTGATG 
CAGTTCGGAA GGATCGACGG GAACGCATAT ATCCTGGACT TTCAGTACCC CTTCTCAGCT 
GTGCAAGCTT TTGCTGTAGC ACTTGCTAAT GTCACCCAGC GCCTCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tulp4a ( XM_017355027.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017210516.1 Danio rerio tubby like protein 4a (tulp4a), transcript variant X2, mRNA.

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CloneID	ODa42658
Clone ID Related Accession (Same CDS sequence)	XM_017355027.1 , XM_017355027.2
Accession Version	XM_017355027.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	tubby-related protein 4 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGCGG CGGTGGAGCA CGGGCCCGTC CTGTGCAGCG ACTCCAACAT CCTGTGCCTG 
TCCTGGAAGG GCCGCGTGCC GCGCAGCCAG AAGGAGAAGC CGGTGTGTCG GCGACGGTAC 
TGCGAGGAGG GCTGGCTGGC CACGGGGAAC GGCAGAGGGG TGGTGGGAGT CACCTTCACC 
TCCAGCCACT GCAGGAGGGA CCGAAACACT CCACAGCGCA TCAACTTCAA CCTCAGGGGA 
CACAACAGTG AGGTGGTGCT GGTGCGCTGG AACGAGCCCT TCCAGAAGCT GGCGACCTGC 
GATACTGACG GTGGGATCTT CGTCTGGATT CAGTATGAGG GGCGCTGGTC GGTGGAGCTG 
GTCAATGATC GCGGAGCTCA GGTGAGCGAC TTCACCTGGT CTCATGATGG GACGCAGGCT 
CTGATCTCGT ACCGTGATGG TTTCGTGTTG GTGGGTTCGG TGAGCGGTCA GCGCCACTGG 
TCCTCCGAGA TCAACCTTGA GAGTCAGATC ACCTGCGGCA TCTGGACGCC CGACGACCAG 
CAGGTGTTGT TTGGCACTGC AGACGGGCAG GTGATCGTGA TGGACTGCCA CGGCCGCATG 
TTGGCTCATA TTCTGCTCCA TGAGGCCGAG GGCATCGCCA GCATGGCCTG GAACTATCCC 
AGCTTCCTGG TGGAGGACAG CAGCGAGAGC GACTCCGATT CAGACGACAG CACGCCTCCG 
CAGGTGCACA CCCTGAAGCC GCTGCTGACC GTCTGCTTCA CGTCCGGAGA CATCAGTTTG 
ATGAGCAATT ATGATGACCT TTCACCTCTG CTGATACGCA CGGGCCTCAA AGATGTGGTG 
GTGCAGTGGT CTTCCCAGGG GGATCTGCTG GCTGTCGCAG GGATGGAGAG ACTCGTGACT 
TCCGACCCCA CACAGGCCAC CCGAAACGCC ATTGTAAAGT TTTACAATGT TCGCGGGGAG 
CACATTTACT CACTGGAAAC ACCTGCTCAG AGGCCGATCA GTACGCTGTG CTGGGGCCAC 
CGGGACTCGC GTGTGTTTCT GGCCTGCGGG CCGGCGCTGT ATGTGCTGCG GGTCGAGCAC 
AGAGTCTCCA GCCTGCAGCT TCTGTGCCAA CAGGCCATCG CCAGTGCCGT ACACGAGGAG 
AAAGATCTGG CCAAGCTCAG CATGCCCTCC CGCCTCAGCA CATATGTGTC TTCTGCCTTC 
GCTCCCACTA TTAAGCCTCC AATCCCAGAT CCCAACAACA TGCGGGACTT TGTTAGCTAT 
CCGACGTCAG GTAATGAGCG TCTGCACTGC ACCATGAAGC GAACGGAGGA CAACCCTGAG 
GTCGGCGGTC CATGCTACAC CTTATACCTG GAGTATCTGG GAGGACTCGT TCCTATTCTG 
AAAGGCCGTA GGATAAGCAA ACTTCGTCCT GAATTTGTCA TCATGGACCC GAAGACTGAT 
GGGAAAACAG ATGAAGTGTA TGGGAACAGC TTCATCCCCT CCATGATTGA CAGCTGCAAT 
TGCTCAGATT CAAGTGACAT TGAGCTCAAT GATGATTGGG TAGGAAAGAA ATCACCAAAG 
ATCTCCAGAG GGAGCAAGTC TCCCAAACTT CCCAGGATAA ATATAGATCC TCGCAAGTCC 
CCCAAGCTGT CCCGCACCAC ACAGGAAATA TCCCGATCGC CACGGTTACC CATTCGCAAG 
CCCTCTATTG GCTCTCCAAG TCTGACTCGC AGAGAGTTCC CACTTGATGA CATCACACAG 
CACAATTACC TTGCGCAAGT TACATCTAAC ATATGGGGAA CAAAGTTCAA AATTGTGGGA 
CTTGCAGCAT TTTTGCCAAC AAATCTTGGT GCAGTTATCT ACAAGACAAG TCTGCTTCAC 
CTCCAGCCCA GACAGATGAC CATCTACCTC CCTGAGGTGA GGAAGATCTC GGTGGAATAC 
ATTAACCTTC CTATGTTCAG TCCTAATGTC TTCAGTGAGG ACGAGGACGA CCTGCCTGTC 
ACAGGAGCTT CAGGAATCAC TGATGATAAT CCACCTTGCA CTGTTAACAT ACCCATTGCC 
CCCATACACA GCCCTGCCCA AGCCATGTCC CCAACTCAAA GCATTGGCCT AGTCCAATCC 
CTACTAGCCA ATCAGAATGT CCAGTTAGAC GTATTGTCCA ACCCAACATC GACTGTGGCT 
CCTCCTGGGG TGTCTGATCA AGGACAGCAT GACACTGTGC TTACGGCACA ATACACTGTT 
CCTGCCAGGT ATTCTAGTCC TGGTCAGGTA ATCTTCGGAG GTATGGATGT TGGTCGGCTT 
TTAGTTGCAC AGTCCCAACA GCAGCAGCAG CAATCACAAC AACATCAGCA GCTGCAACAG 
CATTATCAGC AACAGCAGCT ACAGCAACAG CTGCAACAAT ATCAGCTGCA GCAGCAACAG 
CTGTTGCAGC ATCAGCACAT GCAGCAACAG CAGCAGCAAC ACATGCAGCA GCAGATCCAA 
CAGATGCAGC AACAGCAGCA GATGCAGCAA CAGCTTCAGC ATCAAATACA GCAGCAGCAT 
CTGCAAATGC AACTGCAGCT ACAGCACCAG CAAATGCAGC AGCAGTTGCA ACAGCAGCAT 
CAGATGCAAA TCCCTGTTCC AACTTTGCCC TCAGGTCAAC CATCATGCCC AGTCATTCAG 
CTTCCACAAA GTACAATACC AGTCGCTGCC CCTACTGCAG AGCACAGCAC AGAGCGCGGG 
GAGCATGAGC TCCTGCTTAA AATTAAAACC ACTCGTTCTG CCCCACAACT AGCTGAAGGT 
GATGCAGTGG TTTTTACAGC ACCTCTTGAG ATTAGCAAAA TGAACCCGCC CCCTCCTTAC 
CCTGGCACAG TCGCCGCTGC AGTGGCAGCT GCTACTGCTG CAGTTTCGGC TGCTGCTTCT 
GCAAGTTCCA CCTCTGGGGC AGTGGGAAGC AGCTCTAGCA CGTCATCCTC AGGGGAAGTT 
TGTCTGAAGA AAGGGGATCT GACCTTGTAT CCACCTAGTG CACAAGGGGC TCAGTACCCA 
ACTCCATTGG GCTATGAACG CATCACCACA TTTGACAGCA GTGGAAATGT GGAAGAGGTG 
TGCCGACCCC GCACACGACT TGTTTGCAAC CAGAATGTTT ACACGCTGCA GGGCCCAGGA 
AGCTCTGCCA CCCTCAGGGT CTCATCATCT TCCTCTGCTG TAGAGGGGAA GAAGGTGCAG 
CTGCCCTATG CCTCTGCTAC GTTGAACCGT CTCACTGTGC CAAGGTACTC TATACCCAGT 
GGGGACCCTC CTCCATACCC TGATACAGCT AACCAGGGCG GAGCAGGGCG CAACCCTGGC 
CAGAGACTGG ACAGCAGCTT GATTCATGCT ACCTTGAGGC GCAACAGTCG GGAAGCTGCA 
TTGAAGGTTT CCCAAATGCT GGATCACCAG CGGACTTTAC CCCCCAAGGC TAAAGCCAGT 
ACTTTGTCTG CTTCCTATCA ACCAAGAGGG CCGACAGCTC TTTATACATG CAGTCAGTGC 
AGTAGCAACA GTAGTGGAAC CACAAGCAGT GGAAGTAACG GAATAGCTGG GGGCACAATC 
ATTAGGCAAG ATTTCCCACC AACTGGGGGT GCTGCACCTC ATAGCACTGT TATAGTGCAC 
TCCAACAGTG CCTCGCCTCT GCCTTCTCAA TCCTCCTACA GTCTGCTCAG TCTGGATGGC 
ACCGGTGGAG TGGCTACTGG AGCTGGAAAC AGGGAAAGAG CTGACTATAT CAACTCAGCT 
TTTACAGAGG ACGAGGCATT GAATCAGTCT CTGAGGCAAA TGGCCCTTAA TACCGAGGTA 
GTGGGTTTGA CAGTCAAACG GCCTCCACCG TATCAATGGG ACCCATCTGT TACAGAAGAG 
ATTTGGGTTC CTCAAGAGCG CACAGCGACT CTGCCCCCTT CTGGACCTCC TGCACCTCAC 
AAACCTCCTC CGCTCATCCT CGGTCCTGCA CAGCATCTGG ACGTTTCTCG TCTTCCGTTT 
GTCCTCTCAC CTAAATCTCC AACAAGTCCT AACAACACCA CTTTTCCACT AGCAGGAGCT 
TATCAGATTG CATTGCCCTA TCCATCAAGT GCTGCATACA GTAGTCCCCC ACTGCAGGCA 
GTGCAAGCAT CTCCACGGCC GCCTTCTCCT AAAGAGGCGG TTGCTCCACT TTCCTTTCCC 
CAGCAAGACC AGTCAGTGGT CTTGCCTCCT GGTTATCCAC CAAATTTGCC CAACCTGGCT 
TGCTGTCCCC TCCCACCAAT GTACCCTGGC ACAGCTTCTT GTTCTAGCCT GCCTGTAACC 
GCCATTCCTC TTCATCCATG GGGTTCTTAC AATCCCTGTC CCCCCATGCC AAGCCCCCCG 
GGTCCCGCCC CATCTCTCCC ACCCAAGGCC TCCCACATGC TGGATAAACC TGTTCGTTCT 
CCACCTCCTC AACCGCCGCC TTTGCCTTCG CCTCCCCCAG ACCTCCAGAC TGCGGTCAGT 
CCCCCAGAAG CAATAGCTGA AGCCGGGGAA GGCTTTCAAG AGGTGCTCTC GCTTAACGAA 
AGCCCTGTTC CTCAACGAGC AGACAGGTTT GGCAAGAAGA ATCGAAAGCG TTTGGACAAC 
ACTCGGGCAG ATGAAGCTAA TGTTCCAGCA ATCACAGAGG GAGGCAACAA GTCCAAAAAA 
GAGGGCCGTG CCCTTGGGGA CTTCAACTCC CTCATCTCCA GTCCGAGGTT AGGTGGGAGA 
GATAAAAAGA AACCTAAAGG TCAAAAAGAG CAGCTGAAAG CCAAAAAGCT AAGCAAGGCC 
ACCACAAATG AGTTCCAGGA CAGCTCAGAG AGCGAGCCTG AGCTCTTTAT CAGTGGAGAT 
GAGCTGATGA ACCAGAGCCA GAGTGGGAAG AAGAGTTGGA AAGCCAAAAG GAACCTGCGG 
GCAGGAGGTT GTGAGCTGGA TGAGATCAAA TGTCGAAAAG CGAATGAGAA AGAGGACCGA 
GGCCTCGGCA GCCAGGGCTT CGTCTACATC ATGGCCAACA AACAGCCTCT CTGGAATGAA 
GCCACGCAGG TCTACCAGCT AGACTTTGGA GGGAGAGTCA CACAGGAATC AGCAAAGAAC 
TTCCAGATTG AGCTTGAGGG GAGACAGGTG ATGCAGTTCG GAAGGATCGA CGGGAACGCA 
TATATCCTGG ACTTTCAGTA CCCCTTCTCA GCTGTGCAAG CTTTTGCTGT AGCACTTGCT 
AATGTCACCC AGCGCCTCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210516.1
CDS	696..5879
Translation	MFAAVEHGPVLCSDSNILCLSWKGRVPRSQKEKPVCRRRYCEEGWLATGNGRGVVGVTFTSSHCRRDRNTPQRINFNLRGHNSEVVLVRWNEPFQKLATCDTDGGIFVWIQYEGRWSVELVNDRGAQVSDFTWSHDGTQALISYRDGFVLVGSVSGQRHWSSEINLESQITCGIWTPDDQQVLFGTADGQVIVMDCHGRMLAHILLHEAEGIASMAWNYPSFLVEDSSESDSDSDDSTPPQVHTLKPLLTVCFTSGDISLMSNYDDLSPLLIRTGLKDVVVQWSSQGDLLAVAGMERLVTSDPTQATRNAIVKFYNVRGEHIYSLETPAQRPISTLCWGHRDSRVFLACGPALYVLRVEHRVSSLQLLCQQAIASAVHEEKDLAKLSMPSRLSTYVSSAFAPTIKPPIPDPNNMRDFVSYPTSGNERLHCTMKRTEDNPEVGGPCYTLYLEYLGGLVPILKGRRISKLRPEFVIMDPKTDGKTDEVYGNSFIPSMIDSCNCSDSSDIELNDDWVGKKSPKISRGSKSPKLPRINIDPRKSPKLSRTTQEISRSPRLPIRKPSIGSPSLTRREFPLDDITQHNYLAQVTSNIWGTKFKIVGLAAFLPTNLGAVIYKTSLLHLQPRQMTIYLPEVRKISVEYINLPMFSPNVFSEDEDDLPVTGASGITDDNPPCTVNIPIAPIHSPAQAMSPTQSIGLVQSLLANQNVQLDVLSNPTSTVAPPGVSDQGQHDTVLTAQYTVPARYSSPGQVIFGGMDVGRLLVAQSQQQQQQSQQHQQLQQHYQQQQLQQQLQQYQLQQQQLLQHQHMQQQQQQHMQQQIQQMQQQQQMQQQLQHQIQQQHLQMQLQLQHQQMQQQLQQQHQMQIPVPTLPSGQPSCPVIQLPQSTIPVAAPTAEHSTERGEHELLLKIKTTRSAPQLAEGDAVVFTAPLEISKMNPPPPYPGTVAAAVAAATAAVSAAASASSTSGAVGSSSSTSSSGEVCLKKGDLTLYPPSAQGAQYPTPLGYERITTFDSSGNVEEVCRPRTRLVCNQNVYTLQGPGSSATLRVSSSSSAVEGKKVQLPYASATLNRLTVPRYSIPSGDPPPYPDTANQGGAGRNPGQRLDSSLIHATLRRNSREAALKVSQMLDHQRTLPPKAKASTLSASYQPRGPTALYTCSQCSSNSSGTTSSGSNGIAGGTIIRQDFPPTGGAAPHSTVIVHSNSASPLPSQSSYSLLSLDGTGGVATGAGNRERADYINSAFTEDEALNQSLRQMALNTEVVGLTVKRPPPYQWDPSVTEEIWVPQERTATLPPSGPPAPHKPPPLILGPAQHLDVSRLPFVLSPKSPTSPNNTTFPLAGAYQIALPYPSSAAYSSPPLQAVQASPRPPSPKEAVAPLSFPQQDQSVVLPPGYPPNLPNLACCPLPPMYPGTASCSSLPVTAIPLHPWGSYNPCPPMPSPPGPAPSLPPKASHMLDKPVRSPPPQPPPLPSPPPDLQTAVSPPEAIAEAGEGFQEVLSLNESPVPQRADRFGKKNRKRLDNTRADEANVPAITEGGNKSKKEGRALGDFNSLISSPRLGGRDKKKPKGQKEQLKAKKLSKATTNEFQDSSESEPELFISGDELMNQSQSGKKSWKAKRNLRAGGCELDEIKCRKANEKEDRGLGSQGFVYIMANKQPLWNEATQVYQLDFGGRVTQESAKNFQIELEGRQVMQFGRIDGNAYILDFQYPFSAVQAFAVALANVTQRLK

Target ORF information:

RefSeq Version	XM_017355027.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tubby like protein 4a (tulp4a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017355027.1

ORF Insert Sequence:

ATGTTTGCGG CGGTGGAGCA CGGGCCCGTC CTGTGCAGCG ACTCCAACAT CCTGTGCCTG 
TCCTGGAAGG GCCGCGTGCC GCGCAGCCAG AAGGAGAAGC CGGTGTGTCG GCGACGGTAC 
TGCGAGGAGG GCTGGCTGGC CACGGGGAAC GGCAGAGGGG TGGTGGGAGT CACCTTCACC 
TCCAGCCACT GCAGGAGGGA CCGAAACACT CCACAGCGCA TCAACTTCAA CCTCAGGGGA 
CACAACAGTG AGGTGGTGCT GGTGCGCTGG AACGAGCCCT TCCAGAAGCT GGCGACCTGC 
GATACTGACG GTGGGATCTT CGTCTGGATT CAGTATGAGG GGCGCTGGTC GGTGGAGCTG 
GTCAATGATC GCGGAGCTCA GGTGAGCGAC TTCACCTGGT CTCATGATGG GACGCAGGCT 
CTGATCTCGT ACCGTGATGG TTTCGTGTTG GTGGGTTCGG TGAGCGGTCA GCGCCACTGG 
TCCTCCGAGA TCAACCTTGA GAGTCAGATC ACCTGCGGCA TCTGGACGCC CGACGACCAG 
CAGGTGTTGT TTGGCACTGC AGACGGGCAG GTGATCGTGA TGGACTGCCA CGGCCGCATG 
TTGGCTCATA TTCTGCTCCA TGAGGCCGAG GGCATCGCCA GCATGGCCTG GAACTATCCC 
AGCTTCCTGG TGGAGGACAG CAGCGAGAGC GACTCCGATT CAGACGACAG CACGCCTCCG 
CAGGTGCACA CCCTGAAGCC GCTGCTGACC GTCTGCTTCA CGTCCGGAGA CATCAGTTTG 
ATGAGCAATT ATGATGACCT TTCACCTCTG CTGATACGCA CGGGCCTCAA AGATGTGGTG 
GTGCAGTGGT CTTCCCAGGG GGATCTGCTG GCTGTCGCAG GGATGGAGAG ACTCGTGACT 
TCCGACCCCA CACAGGCCAC CCGAAACGCC ATTGTAAAGT TTTACAATGT TCGCGGGGAG 
CACATTTACT CACTGGAAAC ACCTGCTCAG AGGCCGATCA GTACGCTGTG CTGGGGCCAC 
CGGGACTCGC GTGTGTTTCT GGCCTGCGGG CCGGCGCTGT ATGTGCTGCG GGTCGAGCAC 
AGAGTCTCCA GCCTGCAGCT TCTGTGCCAA CAGGCCATCG CCAGTGCCGT ACACGAGGAG 
AAAGATCTGG CCAAGCTCAG CATGCCCTCC CGCCTCAGCA CATATGTGTC TTCTGCCTTC 
GCTCCCACTA TTAAGCCTCC AATCCCAGAT CCCAACAACA TGCGGGACTT TGTTAGCTAT 
CCGACGTCAG GTAATGAGCG TCTGCACTGC ACCATGAAGC GAACGGAGGA CAACCCTGAG 
GTCGGCGGTC CATGCTACAC CTTATACCTG GAGTATCTGG GAGGACTCGT TCCTATTCTG 
AAAGGCCGTA GGATAAGCAA ACTTCGTCCT GAATTTGTCA TCATGGACCC GAAGACTGAT 
GGGAAAACAG ATGAAGTGTA TGGGAACAGC TTCATCCCCT CCATGATTGA CAGCTGCAAT 
TGCTCAGATT CAAGTGACAT TGAGCTCAAT GATGATTGGG TAGGAAAGAA ATCACCAAAG 
ATCTCCAGAG GGAGCAAGTC TCCCAAACTT CCCAGGATAA ATATAGATCC TCGCAAGTCC 
CCCAAGCTGT CCCGCACCAC ACAGGAAATA TCCCGATCGC CACGGTTACC CATTCGCAAG 
CCCTCTATTG GCTCTCCAAG TCTGACTCGC AGAGAGTTCC CACTTGATGA CATCACACAG 
CACAATTACC TTGCGCAAGT TACATCTAAC ATATGGGGAA CAAAGTTCAA AATTGTGGGA 
CTTGCAGCAT TTTTGCCAAC AAATCTTGGT GCAGTTATCT ACAAGACAAG TCTGCTTCAC 
CTCCAGCCCA GACAGATGAC CATCTACCTC CCTGAGGTGA GGAAGATCTC GGTGGAATAC 
ATTAACCTTC CTATGTTCAG TCCTAATGTC TTCAGTGAGG ACGAGGACGA CCTGCCTGTC 
ACAGGAGCTT CAGGAATCAC TGATGATAAT CCACCTTGCA CTGTTAACAT ACCCATTGCC 
CCCATACACA GCCCTGCCCA AGCCATGTCC CCAACTCAAA GCATTGGCCT AGTCCAATCC 
CTACTAGCCA ATCAGAATGT CCAGTTAGAC GTATTGTCCA ACCCAACATC GACTGTGGCT 
CCTCCTGGGG TGTCTGATCA AGGACAGCAT GACACTGTGC TTACGGCACA ATACACTGTT 
CCTGCCAGGT ATTCTAGTCC TGGTCAGGTA ATCTTCGGAG GTATGGATGT TGGTCGGCTT 
TTAGTTGCAC AGTCCCAACA GCAGCAGCAG CAATCACAAC AACATCAGCA GCTGCAACAG 
CATTATCAGC AACAGCAGCT ACAGCAACAG CTGCAACAAT ATCAGCTGCA GCAGCAACAG 
CTGTTGCAGC ATCAGCACAT GCAGCAACAG CAGCAGCAAC ACATGCAGCA GCAGATCCAA 
CAGATGCAGC AACAGCAGCA GATGCAGCAA CAGCTTCAGC ATCAAATACA GCAGCAGCAT 
CTGCAAATGC AACTGCAGCT ACAGCACCAG CAAATGCAGC AGCAGTTGCA ACAGCAGCAT 
CAGATGCAAA TCCCTGTTCC AACTTTGCCC TCAGGTCAAC CATCATGCCC AGTCATTCAG 
CTTCCACAAA GTACAATACC AGTCGCTGCC CCTACTGCAG AGCACAGCAC AGAGCGCGGG 
GAGCATGAGC TCCTGCTTAA AATTAAAACC ACTCGTTCTG CCCCACAACT AGCTGAAGGT 
GATGCAGTGG TTTTTACAGC ACCTCTTGAG ATTAGCAAAA TGAACCCGCC CCCTCCTTAC 
CCTGGCACAG TCGCCGCTGC AGTGGCAGCT GCTACTGCTG CAGTTTCGGC TGCTGCTTCT 
GCAAGTTCCA CCTCTGGGGC AGTGGGAAGC AGCTCTAGCA CGTCATCCTC AGGGGAAGTT 
TGTCTGAAGA AAGGGGATCT GACCTTGTAT CCACCTAGTG CACAAGGGGC TCAGTACCCA 
ACTCCATTGG GCTATGAACG CATCACCACA TTTGACAGCA GTGGAAATGT GGAAGAGGTG 
TGCCGACCCC GCACACGACT TGTTTGCAAC CAGAATGTTT ACACGCTGCA GGGCCCAGGA 
AGCTCTGCCA CCCTCAGGGT CTCATCATCT TCCTCTGCTG TAGAGGGGAA GAAGGTGCAG 
CTGCCCTATG CCTCTGCTAC GTTGAACCGT CTCACTGTGC CAAGGTACTC TATACCCAGT 
GGGGACCCTC CTCCATACCC TGATACAGCT AACCAGGGCG GAGCAGGGCG CAACCCTGGC 
CAGAGACTGG ACAGCAGCTT GATTCATGCT ACCTTGAGGC GCAACAGTCG GGAAGCTGCA 
TTGAAGGTTT CCCAAATGCT GGATCACCAG CGGACTTTAC CCCCCAAGGC TAAAGCCAGT 
ACTTTGTCTG CTTCCTATCA ACCAAGAGGG CCGACAGCTC TTTATACATG CAGTCAGTGC 
AGTAGCAACA GTAGTGGAAC CACAAGCAGT GGAAGTAACG GAATAGCTGG GGGCACAATC 
ATTAGGCAAG ATTTCCCACC AACTGGGGGT GCTGCACCTC ATAGCACTGT TATAGTGCAC 
TCCAACAGTG CCTCGCCTCT GCCTTCTCAA TCCTCCTACA GTCTGCTCAG TCTGGATGGC 
ACCGGTGGAG TGGCTACTGG AGCTGGAAAC AGGGAAAGAG CTGACTATAT CAACTCAGCT 
TTTACAGAGG ACGAGGCATT GAATCAGTCT CTGAGGCAAA TGGCCCTTAA TACCGAGGTA 
GTGGGTTTGA CAGTCAAACG GCCTCCACCG TATCAATGGG ACCCATCTGT TACAGAAGAG 
ATTTGGGTTC CTCAAGAGCG CACAGCGACT CTGCCCCCTT CTGGACCTCC TGCACCTCAC 
AAACCTCCTC CGCTCATCCT CGGTCCTGCA CAGCATCTGG ACGTTTCTCG TCTTCCGTTT 
GTCCTCTCAC CTAAATCTCC AACAAGTCCT AACAACACCA CTTTTCCACT AGCAGGAGCT 
TATCAGATTG CATTGCCCTA TCCATCAAGT GCTGCATACA GTAGTCCCCC ACTGCAGGCA 
GTGCAAGCAT CTCCACGGCC GCCTTCTCCT AAAGAGGCGG TTGCTCCACT TTCCTTTCCC 
CAGCAAGACC AGTCAGTGGT CTTGCCTCCT GGTTATCCAC CAAATTTGCC CAACCTGGCT 
TGCTGTCCCC TCCCACCAAT GTACCCTGGC ACAGCTTCTT GTTCTAGCCT GCCTGTAACC 
GCCATTCCTC TTCATCCATG GGGTTCTTAC AATCCCTGTC CCCCCATGCC AAGCCCCCCG 
GGTCCCGCCC CATCTCTCCC ACCCAAGGCC TCCCACATGC TGGATAAACC TGTTCGTTCT 
CCACCTCCTC AACCGCCGCC TTTGCCTTCG CCTCCCCCAG ACCTCCAGAC TGCGGTCAGT 
CCCCCAGAAG CAATAGCTGA AGCCGGGGAA GGCTTTCAAG AGGTGCTCTC GCTTAACGAA 
AGCCCTGTTC CTCAACGAGC AGACAGGTTT GGCAAGAAGA ATCGAAAGCG TTTGGACAAC 
ACTCGGGCAG ATGAAGCTAA TGTTCCAGCA ATCACAGAGG GAGGCAACAA GTCCAAAAAA 
GAGGGCCGTG CCCTTGGGGA CTTCAACTCC CTCATCTCCA GTCCGAGGTT AGGTGGGAGA 
GATAAAAAGA AACCTAAAGG TCAAAAAGAG CAGCTGAAAG CCAAAAAGCT AAGCAAGGCC 
ACCACAAATG AGTTCCAGGA CAGCTCAGAG AGCGAGCCTG AGCTCTTTAT CAGTGGAGAT 
GAGCTGATGA ACCAGAGCCA GAGTGGGAAG AAGAGTTGGA AAGCCAAAAG GAACCTGCGG 
GCAGGAGGTT GTGAGCTGGA TGAGATCAAA TGTCGAAAAG CGAATGAGAA AGAGGACCGA 
GGCCTCGGCA GCCAGGGCTT CGTCTACATC ATGGCCAACA AACAGCCTCT CTGGAATGAA 
GCCACGCAGG TCTACCAGCT AGACTTTGGA GGGAGAGTCA CACAGGAATC AGCAAAGAAC 
TTCCAGATTG AGCTTGAGGG GAGACAGGTG ATGCAGTTCG GAAGGATCGA CGGGAACGCA 
TATATCCTGG ACTTTCAGTA CCCCTTCTCA GCTGTGCAAG CTTTTGCTGT AGCACTTGCT 
AATGTCACCC AGCGCCTCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tulp4a ( XM_017355027.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017210516.1 Danio rerio tubby like protein 4a (tulp4a), transcript variant X2, mRNA.

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CloneID	ODa42658
Clone ID Related Accession (Same CDS sequence)	XM_017355027.1 , XM_017355027.2
Accession Version	XM_017355027.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	tubby-related protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017355027.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGCGG CGGTGGAGCA CGGGCCCGTC CTGTGCAGCG ACTCCAACAT CCTGTGCCTG 
TCCTGGAAGG GCCGCGTGCC GCGCAGCCAG AAGGAGAAGC CGGTGTGTCG GCGACGGTAC 
TGCGAGGAGG GCTGGCTGGC CACGGGGAAC GGCAGAGGGG TGGTGGGAGT CACCTTCACC 
TCCAGCCACT GCAGGAGGGA CCGAAACACT CCACAGCGCA TCAACTTCAA CCTCAGGGGA 
CACAACAGTG AGGTGGTGCT GGTGCGCTGG AACGAGCCCT TCCAGAAGCT GGCGACCTGC 
GATACTGACG GTGGGATCTT CGTCTGGATT CAGTATGAGG GGCGCTGGTC GGTGGAGCTG 
GTCAATGATC GCGGAGCTCA GGTGAGCGAC TTCACCTGGT CTCATGATGG GACGCAGGCT 
CTGATCTCGT ACCGTGATGG TTTCGTGTTG GTGGGTTCGG TGAGCGGTCA GCGCCACTGG 
TCCTCCGAGA TCAACCTTGA GAGTCAGATC ACCTGCGGCA TCTGGACGCC CGACGACCAG 
CAGGTGTTGT TTGGCACTGC AGACGGGCAG GTGATCGTGA TGGACTGCCA CGGCCGCATG 
TTGGCTCATA TTCTGCTCCA TGAGGCCGAG GGCATCGCCA GCATGGCCTG GAACTATCCC 
AGCTTCCTGG TGGAGGACAG CAGCGAGAGC GACTCCGATT CAGACGACAG CACGCCTCCG 
CAGGTGCACA CCCTGAAGCC GCTGCTGACC GTCTGCTTCA CGTCCGGAGA CATCAGTTTG 
ATGAGCAATT ATGATGACCT TTCACCTCTG CTGATACGCA CGGGCCTCAA AGATGTGGTG 
GTGCAGTGGT CTTCCCAGGG GGATCTGCTG GCTGTCGCAG GGATGGAGAG ACTCGTGACT 
TCCGACCCCA CACAGGCCAC CCGAAACGCC ATTGTAAAGT TTTACAATGT TCGCGGGGAG 
CACATTTACT CACTGGAAAC ACCTGCTCAG AGGCCGATCA GTACGCTGTG CTGGGGCCAC 
CGGGACTCGC GTGTGTTTCT GGCCTGCGGG CCGGCGCTGT ATGTGCTGCG GGTCGAGCAC 
AGAGTCTCCA GCCTGCAGCT TCTGTGCCAA CAGGCCATCG CCAGTGCCGT ACACGAGGAG 
AAAGATCTGG CCAAGCTCAG CATGCCCTCC CGCCTCAGCA CATATGTGTC TTCTGCCTTC 
GCTCCCACTA TTAAGCCTCC AATCCCAGAT CCCAACAACA TGCGGGACTT TGTTAGCTAT 
CCGACGTCAG GTAATGAGCG TCTGCACTGC ACCATGAAGC GAACGGAGGA CAACCCTGAG 
GTCGGCGGTC CATGCTACAC CTTATACCTG GAGTATCTGG GAGGACTCGT TCCTATTCTG 
AAAGGCCGTA GGATAAGCAA ACTTCGTCCT GAATTTGTCA TCATGGACCC GAAGACTGAT 
GGGAAAACAG ATGAAGTGTA TGGGAACAGC TTCATCCCCT CCATGATTGA CAGCTGCAAT 
TGCTCAGATT CAAGTGACAT TGAGCTCAAT GATGATTGGG TAGGAAAGAA ATCACCAAAG 
ATCTCCAGAG GGAGCAAGTC TCCCAAACTT CCCAGGATAA ATATAGATCC TCGCAAGTCC 
CCCAAGCTGT CCCGCACCAC ACAGGAAATA TCCCGATCGC CACGGTTACC CATTCGCAAG 
CCCTCTATTG GCTCTCCAAG TCTGACTCGC AGAGAGTTCC CACTTGATGA CATCACACAG 
CACAATTACC TTGCGCAAGT TACATCTAAC ATATGGGGAA CAAAGTTCAA AATTGTGGGA 
CTTGCAGCAT TTTTGCCAAC AAATCTTGGT GCAGTTATCT ACAAGACAAG TCTGCTTCAC 
CTCCAGCCCA GACAGATGAC CATCTACCTC CCTGAGGTGA GGAAGATCTC GGTGGAATAC 
ATTAACCTTC CTATGTTCAG TCCTAATGTC TTCAGTGAGG ACGAGGACGA CCTGCCTGTC 
ACAGGAGCTT CAGGAATCAC TGATGATAAT CCACCTTGCA CTGTTAACAT ACCCATTGCC 
CCCATACACA GCCCTGCCCA AGCCATGTCC CCAACTCAAA GCATTGGCCT AGTCCAATCC 
CTACTAGCCA ATCAGAATGT CCAGTTAGAC GTATTGTCCA ACCCAACATC GACTGTGGCT 
CCTCCTGGGG TGTCTGATCA AGGACAGCAT GACACTGTGC TTACGGCACA ATACACTGTT 
CCTGCCAGGT ATTCTAGTCC TGGTCAGGTA ATCTTCGGAG GTATGGATGT TGGTCGGCTT 
TTAGTTGCAC AGTCCCAACA GCAGCAGCAG CAATCACAAC AACATCAGCA GCTGCAACAG 
CATTATCAGC AACAGCAGCT ACAGCAACAG CTGCAACAAT ATCAGCTGCA GCAGCAACAG 
CTGTTGCAGC ATCAGCACAT GCAGCAACAG CAGCAGCAAC ACATGCAGCA GCAGATCCAA 
CAGATGCAGC AACAGCAGCA GATGCAGCAA CAGCTTCAGC ATCAAATACA GCAGCAGCAT 
CTGCAAATGC AACTGCAGCT ACAGCACCAG CAAATGCAGC AGCAGTTGCA ACAGCAGCAT 
CAGATGCAAA TCCCTGTTCC AACTTTGCCC TCAGGTCAAC CATCATGCCC AGTCATTCAG 
CTTCCACAAA GTACAATACC AGTCGCTGCC CCTACTGCAG AGCACAGCAC AGAGCGCGGG 
GAGCATGAGC TCCTGCTTAA AATTAAAACC ACTCGTTCTG CCCCACAACT AGCTGAAGGT 
GATGCAGTGG TTTTTACAGC ACCTCTTGAG ATTAGCAAAA TGAACCCGCC CCCTCCTTAC 
CCTGGCACAG TCGCCGCTGC AGTGGCAGCT GCTACTGCTG CAGTTTCGGC TGCTGCTTCT 
GCAAGTTCCA CCTCTGGGGC AGTGGGAAGC AGCTCTAGCA CGTCATCCTC AGGGGAAGTT 
TGTCTGAAGA AAGGGGATCT GACCTTGTAT CCACCTAGTG CACAAGGGGC TCAGTACCCA 
ACTCCATTGG GCTATGAACG CATCACCACA TTTGACAGCA GTGGAAATGT GGAAGAGGTG 
TGCCGACCCC GCACACGACT TGTTTGCAAC CAGAATGTTT ACACGCTGCA GGGCCCAGGA 
AGCTCTGCCA CCCTCAGGGT CTCATCATCT TCCTCTGCTG TAGAGGGGAA GAAGGTGCAG 
CTGCCCTATG CCTCTGCTAC GTTGAACCGT CTCACTGTGC CAAGGTACTC TATACCCAGT 
GGGGACCCTC CTCCATACCC TGATACAGCT AACCAGGGCG GAGCAGGGCG CAACCCTGGC 
CAGAGACTGG ACAGCAGCTT GATTCATGCT ACCTTGAGGC GCAACAGTCG GGAAGCTGCA 
TTGAAGGTTT CCCAAATGCT GGATCACCAG CGGACTTTAC CCCCCAAGGC TAAAGCCAGT 
ACTTTGTCTG CTTCCTATCA ACCAAGAGGG CCGACAGCTC TTTATACATG CAGTCAGTGC 
AGTAGCAACA GTAGTGGAAC CACAAGCAGT GGAAGTAACG GAATAGCTGG GGGCACAATC 
ATTAGGCAAG ATTTCCCACC AACTGGGGGT GCTGCACCTC ATAGCACTGT TATAGTGCAC 
TCCAACAGTG CCTCGCCTCT GCCTTCTCAA TCCTCCTACA GTCTGCTCAG TCTGGATGGC 
ACCGGTGGAG TGGCTACTGG AGCTGGAAAC AGGGAAAGAG CTGACTATAT CAACTCAGCT 
TTTACAGAGG ACGAGGCATT GAATCAGTCT CTGAGGCAAA TGGCCCTTAA TACCGAGGTA 
GTGGGTTTGA CAGTCAAACG GCCTCCACCG TATCAATGGG ACCCATCTGT TACAGAAGAG 
ATTTGGGTTC CTCAAGAGCG CACAGCGACT CTGCCCCCTT CTGGACCTCC TGCACCTCAC 
AAACCTCCTC CGCTCATCCT CGGTCCTGCA CAGCATCTGG ACGTTTCTCG TCTTCCGTTT 
GTCCTCTCAC CTAAATCTCC AACAAGTCCT AACAACACCA CTTTTCCACT AGCAGGAGCT 
TATCAGATTG CATTGCCCTA TCCATCAAGT GCTGCATACA GTAGTCCCCC ACTGCAGGCA 
GTGCAAGCAT CTCCACGGCC GCCTTCTCCT AAAGAGGCGG TTGCTCCACT TTCCTTTCCC 
CAGCAAGACC AGTCAGTGGT CTTGCCTCCT GGTTATCCAC CAAATTTGCC CAACCTGGCT 
TGCTGTCCCC TCCCACCAAT GTACCCTGGC ACAGCTTCTT GTTCTAGCCT GCCTGTAACC 
GCCATTCCTC TTCATCCATG GGGTTCTTAC AATCCCTGTC CCCCCATGCC AAGCCCCCCG 
GGTCCCGCCC CATCTCTCCC ACCCAAGGCC TCCCACATGC TGGATAAACC TGTTCGTTCT 
CCACCTCCTC AACCGCCGCC TTTGCCTTCG CCTCCCCCAG ACCTCCAGAC TGCGGTCAGT 
CCCCCAGAAG CAATAGCTGA AGCCGGGGAA GGCTTTCAAG AGGTGCTCTC GCTTAACGAA 
AGCCCTGTTC CTCAACGAGC AGACAGGTTT GGCAAGAAGA ATCGAAAGCG TTTGGACAAC 
ACTCGGGCAG ATGAAGCTAA TGTTCCAGCA ATCACAGAGG GAGGCAACAA GTCCAAAAAA 
GAGGGCCGTG CCCTTGGGGA CTTCAACTCC CTCATCTCCA GTCCGAGGTT AGGTGGGAGA 
GATAAAAAGA AACCTAAAGG TCAAAAAGAG CAGCTGAAAG CCAAAAAGCT AAGCAAGGCC 
ACCACAAATG AGTTCCAGGA CAGCTCAGAG AGCGAGCCTG AGCTCTTTAT CAGTGGAGAT 
GAGCTGATGA ACCAGAGCCA GAGTGGGAAG AAGAGTTGGA AAGCCAAAAG GAACCTGCGG 
GCAGGAGGTT GTGAGCTGGA TGAGATCAAA TGTCGAAAAG CGAATGAGAA AGAGGACCGA 
GGCCTCGGCA GCCAGGGCTT CGTCTACATC ATGGCCAACA AACAGCCTCT CTGGAATGAA 
GCCACGCAGG TCTACCAGCT AGACTTTGGA GGGAGAGTCA CACAGGAATC AGCAAAGAAC 
TTCCAGATTG AGCTTGAGGG GAGACAGGTG ATGCAGTTCG GAAGGATCGA CGGGAACGCA 
TATATCCTGG ACTTTCAGTA CCCCTTCTCA GCTGTGCAAG CTTTTGCTGT AGCACTTGCT 
AATGTCACCC AGCGCCTCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210516.1
CDS	701..5884
Translation	MFAAVEHGPVLCSDSNILCLSWKGRVPRSQKEKPVCRRRYCEEGWLATGNGRGVVGVTFTSSHCRRDRNTPQRINFNLRGHNSEVVLVRWNEPFQKLATCDTDGGIFVWIQYEGRWSVELVNDRGAQVSDFTWSHDGTQALISYRDGFVLVGSVSGQRHWSSEINLESQITCGIWTPDDQQVLFGTADGQVIVMDCHGRMLAHILLHEAEGIASMAWNYPSFLVEDSSESDSDSDDSTPPQVHTLKPLLTVCFTSGDISLMSNYDDLSPLLIRTGLKDVVVQWSSQGDLLAVAGMERLVTSDPTQATRNAIVKFYNVRGEHIYSLETPAQRPISTLCWGHRDSRVFLACGPALYVLRVEHRVSSLQLLCQQAIASAVHEEKDLAKLSMPSRLSTYVSSAFAPTIKPPIPDPNNMRDFVSYPTSGNERLHCTMKRTEDNPEVGGPCYTLYLEYLGGLVPILKGRRISKLRPEFVIMDPKTDGKTDEVYGNSFIPSMIDSCNCSDSSDIELNDDWVGKKSPKISRGSKSPKLPRINIDPRKSPKLSRTTQEISRSPRLPIRKPSIGSPSLTRREFPLDDITQHNYLAQVTSNIWGTKFKIVGLAAFLPTNLGAVIYKTSLLHLQPRQMTIYLPEVRKISVEYINLPMFSPNVFSEDEDDLPVTGASGITDDNPPCTVNIPIAPIHSPAQAMSPTQSIGLVQSLLANQNVQLDVLSNPTSTVAPPGVSDQGQHDTVLTAQYTVPARYSSPGQVIFGGMDVGRLLVAQSQQQQQQSQQHQQLQQHYQQQQLQQQLQQYQLQQQQLLQHQHMQQQQQQHMQQQIQQMQQQQQMQQQLQHQIQQQHLQMQLQLQHQQMQQQLQQQHQMQIPVPTLPSGQPSCPVIQLPQSTIPVAAPTAEHSTERGEHELLLKIKTTRSAPQLAEGDAVVFTAPLEISKMNPPPPYPGTVAAAVAAATAAVSAAASASSTSGAVGSSSSTSSSGEVCLKKGDLTLYPPSAQGAQYPTPLGYERITTFDSSGNVEEVCRPRTRLVCNQNVYTLQGPGSSATLRVSSSSSAVEGKKVQLPYASATLNRLTVPRYSIPSGDPPPYPDTANQGGAGRNPGQRLDSSLIHATLRRNSREAALKVSQMLDHQRTLPPKAKASTLSASYQPRGPTALYTCSQCSSNSSGTTSSGSNGIAGGTIIRQDFPPTGGAAPHSTVIVHSNSASPLPSQSSYSLLSLDGTGGVATGAGNRERADYINSAFTEDEALNQSLRQMALNTEVVGLTVKRPPPYQWDPSVTEEIWVPQERTATLPPSGPPAPHKPPPLILGPAQHLDVSRLPFVLSPKSPTSPNNTTFPLAGAYQIALPYPSSAAYSSPPLQAVQASPRPPSPKEAVAPLSFPQQDQSVVLPPGYPPNLPNLACCPLPPMYPGTASCSSLPVTAIPLHPWGSYNPCPPMPSPPGPAPSLPPKASHMLDKPVRSPPPQPPPLPSPPPDLQTAVSPPEAIAEAGEGFQEVLSLNESPVPQRADRFGKKNRKRLDNTRADEANVPAITEGGNKSKKEGRALGDFNSLISSPRLGGRDKKKPKGQKEQLKAKKLSKATTNEFQDSSESEPELFISGDELMNQSQSGKKSWKAKRNLRAGGCELDEIKCRKANEKEDRGLGSQGFVYIMANKQPLWNEATQVYQLDFGGRVTQESAKNFQIELEGRQVMQFGRIDGNAYILDFQYPFSAVQAFAVALANVTQRLK

Target ORF information:

RefSeq Version	XM_017355027.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tubby like protein 4a (tulp4a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017355027.2

ORF Insert Sequence:

ATGTTTGCGG CGGTGGAGCA CGGGCCCGTC CTGTGCAGCG ACTCCAACAT CCTGTGCCTG 
TCCTGGAAGG GCCGCGTGCC GCGCAGCCAG AAGGAGAAGC CGGTGTGTCG GCGACGGTAC 
TGCGAGGAGG GCTGGCTGGC CACGGGGAAC GGCAGAGGGG TGGTGGGAGT CACCTTCACC 
TCCAGCCACT GCAGGAGGGA CCGAAACACT CCACAGCGCA TCAACTTCAA CCTCAGGGGA 
CACAACAGTG AGGTGGTGCT GGTGCGCTGG AACGAGCCCT TCCAGAAGCT GGCGACCTGC 
GATACTGACG GTGGGATCTT CGTCTGGATT CAGTATGAGG GGCGCTGGTC GGTGGAGCTG 
GTCAATGATC GCGGAGCTCA GGTGAGCGAC TTCACCTGGT CTCATGATGG GACGCAGGCT 
CTGATCTCGT ACCGTGATGG TTTCGTGTTG GTGGGTTCGG TGAGCGGTCA GCGCCACTGG 
TCCTCCGAGA TCAACCTTGA GAGTCAGATC ACCTGCGGCA TCTGGACGCC CGACGACCAG 
CAGGTGTTGT TTGGCACTGC AGACGGGCAG GTGATCGTGA TGGACTGCCA CGGCCGCATG 
TTGGCTCATA TTCTGCTCCA TGAGGCCGAG GGCATCGCCA GCATGGCCTG GAACTATCCC 
AGCTTCCTGG TGGAGGACAG CAGCGAGAGC GACTCCGATT CAGACGACAG CACGCCTCCG 
CAGGTGCACA CCCTGAAGCC GCTGCTGACC GTCTGCTTCA CGTCCGGAGA CATCAGTTTG 
ATGAGCAATT ATGATGACCT TTCACCTCTG CTGATACGCA CGGGCCTCAA AGATGTGGTG 
GTGCAGTGGT CTTCCCAGGG GGATCTGCTG GCTGTCGCAG GGATGGAGAG ACTCGTGACT 
TCCGACCCCA CACAGGCCAC CCGAAACGCC ATTGTAAAGT TTTACAATGT TCGCGGGGAG 
CACATTTACT CACTGGAAAC ACCTGCTCAG AGGCCGATCA GTACGCTGTG CTGGGGCCAC 
CGGGACTCGC GTGTGTTTCT GGCCTGCGGG CCGGCGCTGT ATGTGCTGCG GGTCGAGCAC 
AGAGTCTCCA GCCTGCAGCT TCTGTGCCAA CAGGCCATCG CCAGTGCCGT ACACGAGGAG 
AAAGATCTGG CCAAGCTCAG CATGCCCTCC CGCCTCAGCA CATATGTGTC TTCTGCCTTC 
GCTCCCACTA TTAAGCCTCC AATCCCAGAT CCCAACAACA TGCGGGACTT TGTTAGCTAT 
CCGACGTCAG GTAATGAGCG TCTGCACTGC ACCATGAAGC GAACGGAGGA CAACCCTGAG 
GTCGGCGGTC CATGCTACAC CTTATACCTG GAGTATCTGG GAGGACTCGT TCCTATTCTG 
AAAGGCCGTA GGATAAGCAA ACTTCGTCCT GAATTTGTCA TCATGGACCC GAAGACTGAT 
GGGAAAACAG ATGAAGTGTA TGGGAACAGC TTCATCCCCT CCATGATTGA CAGCTGCAAT 
TGCTCAGATT CAAGTGACAT TGAGCTCAAT GATGATTGGG TAGGAAAGAA ATCACCAAAG 
ATCTCCAGAG GGAGCAAGTC TCCCAAACTT CCCAGGATAA ATATAGATCC TCGCAAGTCC 
CCCAAGCTGT CCCGCACCAC ACAGGAAATA TCCCGATCGC CACGGTTACC CATTCGCAAG 
CCCTCTATTG GCTCTCCAAG TCTGACTCGC AGAGAGTTCC CACTTGATGA CATCACACAG 
CACAATTACC TTGCGCAAGT TACATCTAAC ATATGGGGAA CAAAGTTCAA AATTGTGGGA 
CTTGCAGCAT TTTTGCCAAC AAATCTTGGT GCAGTTATCT ACAAGACAAG TCTGCTTCAC 
CTCCAGCCCA GACAGATGAC CATCTACCTC CCTGAGGTGA GGAAGATCTC GGTGGAATAC 
ATTAACCTTC CTATGTTCAG TCCTAATGTC TTCAGTGAGG ACGAGGACGA CCTGCCTGTC 
ACAGGAGCTT CAGGAATCAC TGATGATAAT CCACCTTGCA CTGTTAACAT ACCCATTGCC 
CCCATACACA GCCCTGCCCA AGCCATGTCC CCAACTCAAA GCATTGGCCT AGTCCAATCC 
CTACTAGCCA ATCAGAATGT CCAGTTAGAC GTATTGTCCA ACCCAACATC GACTGTGGCT 
CCTCCTGGGG TGTCTGATCA AGGACAGCAT GACACTGTGC TTACGGCACA ATACACTGTT 
CCTGCCAGGT ATTCTAGTCC TGGTCAGGTA ATCTTCGGAG GTATGGATGT TGGTCGGCTT 
TTAGTTGCAC AGTCCCAACA GCAGCAGCAG CAATCACAAC AACATCAGCA GCTGCAACAG 
CATTATCAGC AACAGCAGCT ACAGCAACAG CTGCAACAAT ATCAGCTGCA GCAGCAACAG 
CTGTTGCAGC ATCAGCACAT GCAGCAACAG CAGCAGCAAC ACATGCAGCA GCAGATCCAA 
CAGATGCAGC AACAGCAGCA GATGCAGCAA CAGCTTCAGC ATCAAATACA GCAGCAGCAT 
CTGCAAATGC AACTGCAGCT ACAGCACCAG CAAATGCAGC AGCAGTTGCA ACAGCAGCAT 
CAGATGCAAA TCCCTGTTCC AACTTTGCCC TCAGGTCAAC CATCATGCCC AGTCATTCAG 
CTTCCACAAA GTACAATACC AGTCGCTGCC CCTACTGCAG AGCACAGCAC AGAGCGCGGG 
GAGCATGAGC TCCTGCTTAA AATTAAAACC ACTCGTTCTG CCCCACAACT AGCTGAAGGT 
GATGCAGTGG TTTTTACAGC ACCTCTTGAG ATTAGCAAAA TGAACCCGCC CCCTCCTTAC 
CCTGGCACAG TCGCCGCTGC AGTGGCAGCT GCTACTGCTG CAGTTTCGGC TGCTGCTTCT 
GCAAGTTCCA CCTCTGGGGC AGTGGGAAGC AGCTCTAGCA CGTCATCCTC AGGGGAAGTT 
TGTCTGAAGA AAGGGGATCT GACCTTGTAT CCACCTAGTG CACAAGGGGC TCAGTACCCA 
ACTCCATTGG GCTATGAACG CATCACCACA TTTGACAGCA GTGGAAATGT GGAAGAGGTG 
TGCCGACCCC GCACACGACT TGTTTGCAAC CAGAATGTTT ACACGCTGCA GGGCCCAGGA 
AGCTCTGCCA CCCTCAGGGT CTCATCATCT TCCTCTGCTG TAGAGGGGAA GAAGGTGCAG 
CTGCCCTATG CCTCTGCTAC GTTGAACCGT CTCACTGTGC CAAGGTACTC TATACCCAGT 
GGGGACCCTC CTCCATACCC TGATACAGCT AACCAGGGCG GAGCAGGGCG CAACCCTGGC 
CAGAGACTGG ACAGCAGCTT GATTCATGCT ACCTTGAGGC GCAACAGTCG GGAAGCTGCA 
TTGAAGGTTT CCCAAATGCT GGATCACCAG CGGACTTTAC CCCCCAAGGC TAAAGCCAGT 
ACTTTGTCTG CTTCCTATCA ACCAAGAGGG CCGACAGCTC TTTATACATG CAGTCAGTGC 
AGTAGCAACA GTAGTGGAAC CACAAGCAGT GGAAGTAACG GAATAGCTGG GGGCACAATC 
ATTAGGCAAG ATTTCCCACC AACTGGGGGT GCTGCACCTC ATAGCACTGT TATAGTGCAC 
TCCAACAGTG CCTCGCCTCT GCCTTCTCAA TCCTCCTACA GTCTGCTCAG TCTGGATGGC 
ACCGGTGGAG TGGCTACTGG AGCTGGAAAC AGGGAAAGAG CTGACTATAT CAACTCAGCT 
TTTACAGAGG ACGAGGCATT GAATCAGTCT CTGAGGCAAA TGGCCCTTAA TACCGAGGTA 
GTGGGTTTGA CAGTCAAACG GCCTCCACCG TATCAATGGG ACCCATCTGT TACAGAAGAG 
ATTTGGGTTC CTCAAGAGCG CACAGCGACT CTGCCCCCTT CTGGACCTCC TGCACCTCAC 
AAACCTCCTC CGCTCATCCT CGGTCCTGCA CAGCATCTGG ACGTTTCTCG TCTTCCGTTT 
GTCCTCTCAC CTAAATCTCC AACAAGTCCT AACAACACCA CTTTTCCACT AGCAGGAGCT 
TATCAGATTG CATTGCCCTA TCCATCAAGT GCTGCATACA GTAGTCCCCC ACTGCAGGCA 
GTGCAAGCAT CTCCACGGCC GCCTTCTCCT AAAGAGGCGG TTGCTCCACT TTCCTTTCCC 
CAGCAAGACC AGTCAGTGGT CTTGCCTCCT GGTTATCCAC CAAATTTGCC CAACCTGGCT 
TGCTGTCCCC TCCCACCAAT GTACCCTGGC ACAGCTTCTT GTTCTAGCCT GCCTGTAACC 
GCCATTCCTC TTCATCCATG GGGTTCTTAC AATCCCTGTC CCCCCATGCC AAGCCCCCCG 
GGTCCCGCCC CATCTCTCCC ACCCAAGGCC TCCCACATGC TGGATAAACC TGTTCGTTCT 
CCACCTCCTC AACCGCCGCC TTTGCCTTCG CCTCCCCCAG ACCTCCAGAC TGCGGTCAGT 
CCCCCAGAAG CAATAGCTGA AGCCGGGGAA GGCTTTCAAG AGGTGCTCTC GCTTAACGAA 
AGCCCTGTTC CTCAACGAGC AGACAGGTTT GGCAAGAAGA ATCGAAAGCG TTTGGACAAC 
ACTCGGGCAG ATGAAGCTAA TGTTCCAGCA ATCACAGAGG GAGGCAACAA GTCCAAAAAA 
GAGGGCCGTG CCCTTGGGGA CTTCAACTCC CTCATCTCCA GTCCGAGGTT AGGTGGGAGA 
GATAAAAAGA AACCTAAAGG TCAAAAAGAG CAGCTGAAAG CCAAAAAGCT AAGCAAGGCC 
ACCACAAATG AGTTCCAGGA CAGCTCAGAG AGCGAGCCTG AGCTCTTTAT CAGTGGAGAT 
GAGCTGATGA ACCAGAGCCA GAGTGGGAAG AAGAGTTGGA AAGCCAAAAG GAACCTGCGG 
GCAGGAGGTT GTGAGCTGGA TGAGATCAAA TGTCGAAAAG CGAATGAGAA AGAGGACCGA 
GGCCTCGGCA GCCAGGGCTT CGTCTACATC ATGGCCAACA AACAGCCTCT CTGGAATGAA 
GCCACGCAGG TCTACCAGCT AGACTTTGGA GGGAGAGTCA CACAGGAATC AGCAAAGAAC 
TTCCAGATTG AGCTTGAGGG GAGACAGGTG ATGCAGTTCG GAAGGATCGA CGGGAACGCA 
TATATCCTGG ACTTTCAGTA CCCCTTCTCA GCTGTGCAAG CTTTTGCTGT AGCACTTGCT 
AATGTCACCC AGCGCCTCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Efficient genome-wide mutagenesis of zebrafish genes by retroviral insertions.
Proceedings of the National Academy of Sciences of the United States of America104(30)12428-33(2007 Jul)
Wang D,Jao LE,Zheng N,Dolan K,Ivey J,Zonies S,Wu X,Wu K,Yang H,Meng Q,Zhu Z,Zhang B,Lin S,Burgess SM

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tulp4a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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