pi4kaa cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	pi4kaa
Entrez Gene ID	556956
Full Name	phosphatidylinositol 4-kinase, catalytic, alpha a
Synonyms	pi4ka
General protein information	Preferred Names phosphatidylinositol 4-kinase, catalytic, alpha a Names phosphatidylinositol 4-kinase alpha PI4KIII-alpha phosphatidylinositol 4-kinase III alpha
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005172596.3	XP_005172653.1	phosphatidylinositol 4-kinase alpha isoform X2
XM_005172596.4	XP_005172653.1	phosphatidylinositol 4-kinase alpha isoform X2
XM_009305081.3	XP_009303356.1	phosphatidylinositol 4-kinase alpha isoform X1
XM_009305081.2	XP_009303356.1	phosphatidylinositol 4-kinase alpha isoform X1
NM_001034972.1	NP_001030144.1	phosphatidylinositol 4-kinase alpha

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	PI4KA	XP_001086542.2
Gallus gallus (chicken)	PI4KA	XP_004945537.1
Bos taurus (cattle)	PI4KA	NP_777002.1
Drosophila melanogaster (fruit fly)	PI4KIIIalpha	NP_570014.3
Oryza sativa (rice)	Os03g0711200	NP_001051054.1
Caenorhabditis elegans (roundworm)	CELE_Y75B8A.24	NP_499596.2
Xenopus tropicalis (tropical clawed frog)	pi4ka	NP_001238804.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010801	XP_318507.4
Homo sapiens (human)	PI4KA	NP_477352.3
Pan troglodytes (chimpanzee)	PI4KA	XP_525639.3
Mus musculus (house mouse)	Pi4ka	NP_001001983.2
Canis lupus familiaris (dog)	PI4KA	XP_543569.5
Rattus norvegicus (Norway rat)	Pi4ka	NP_071637.1
Danio rerio (zebrafish)	pi4kaa	NP_001030144.1
Arabidopsis thaliana (thale cress)	ATPI4K ALPHA	NP_175359.2

Gene Ontology
Bibliography

Related articles in PubMed

A Forward Genetic Screen Targeting the Endothelium Reveals a Regulatory Role for the Lipid Kinase Pi4ka in Myelo- and Erythropoiesis.
Ziyad S, Riordan JD, Cavanaugh AM, Su T, Hernandez GE, Hilfenhaus G, Morselli M, Huynh K, Wang K, Chen JN, Dupuy AJ, Iruela-Arispe ML
Cell reports22(5)1211-1224(2018 01)

Evolution of complexity in the zebrafish synapse proteome.
Bayés À, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 03)

Genetic Drivers of Kidney Defects in the DiGeorge Syndrome.
Lopez-Rivera E, Liu YP, Verbitsky M, Anderson BR, Capone VP, Otto EA, Yan Z, Mitrotti A, Martino J, Steers NJ, Fasel DA, Vukojevic K, Deng R, Racedo SE, Liu Q, Werth M, Westland R, Vivante A, Makar GS, Bodria M, Sampson MG, Gillies CE, Vega-Warner V, Maiorana M, Petrey DS, Honig B, Lozanovski VJ, Salomon R, Heidet L, Carpentier W, Gaillard D, Carrea A, Gesualdo L, Cusi D, Izzi C, Scolari F, van Wijk JA, Arapovic A, Saraga-Babic M, Saraga M, Kunac N, Samii A, McDonald-McGinn DM, Crowley TB, Zackai EH, Drozdz D, Miklaszewska M, Tkaczyk M, Sikora P, Szczepanska M, Mizerska-Wasiak M, Krzemien G, Szmigielska A, Zaniew M, Darlow JM, Puri P, Barton D, Casolari E, Furth SL, Warady BA, Gucev Z, Hakonarson H, Flogelova H, Tasic V, Latos-Bielenska A, Materna-Kiryluk A, Allegri L, Wong CS, Drummond IA, D'Agati V, Imamoto A, Barasch JM, Hildebrandt F, Kiryluk K, Lifton RP, Morrow BE, Jeanpierre C, Papaioannou VE, Ghiggeri GM, Gharavi AG, Katsanis N, Sanna-Cherchi S
The New England journal of medicine376(8)742-754(2017 02)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following pi4kaa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pi4kaa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa34570	NM_001034972.1 Latest version!	Danio rerio phosphatidylinositol 4-kinase, catalytic, alpha a (pi4kaa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODa15706	XM_005172596.4 Latest version!	Danio rerio phosphatidylinositol 4-kinase, catalytic, alpha a (pi4kaa), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODa42467	XM_009305081.3 Latest version!	Danio rerio phosphatidylinositol 4-kinase, catalytic, alpha a (pi4kaa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
ODa15706	XM_005172596.3	Danio rerio phosphatidylinositol 4-kinase, catalytic, alpha a (pi4kaa), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODa42467	XM_009305081.2	Danio rerio phosphatidylinositol 4-kinase, catalytic, alpha a (pi4kaa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pi4kaa ( NM_001034972.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001030144.1 Danio rerio phosphatidylinositol 4-kinase, catalytic, alpha a (pi4kaa), mRNA.

Price & Availability↑

CloneID	ODa34570
Clone ID Related Accession (Same CDS sequence)	NM_001034972.1
Accession Version	NM_001034972.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6180bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1572624000000
Organism	Danio rerio(zebrafish)
Product	phosphatidylinositol 4-kinase alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY929292.1. On Oct 22, 2005 this sequence version replaced XM_679896.1. ##Evidence-Data-START## Transcript exon combination :: AY929292.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTGTCCTG TGGACATCCG TGGTGTTTTC CAGCTGGACG AGCGGAGGAG AGATGCTGTG 
ATCGCACTTG GGATCTTCCT AGTGGAGTCT GAGTTGCAGC ACAAGGATGC TATTGTCCCG 
TACTTGTTGG TTTTGCTGAA GGGTTTGCCC AGAGTGCAAT GGATCGAGGA GAGCTCAGAA 
AGGAAAGGAC GAGAAGCGCT TCCCATTGCG GAGAATTTCT CCTTCTGTTT GGTGACGGTG 
CTCTCAGACG TGGCCCAGAT GGATGAATCC ATGCGCATCC AGATTTTGGA GGCTGTGATG 
AACCTCATTC AGGTGCTACA GGAAACATGT CAGAACATCG AGGGCCAAGA TAAAGAATAC 
CTGTGTAAAT ACACCGTGCC CTGCCTGCTC GGTGTGGTGC GAGCGTTTGG CCGCTACAGC 
AACACAGAGG AGCCCCTGTT GTCTAAACTC TTCCCCCGGA TCGTTCCTCA GCCTCCAGGC 
ACCGGCGACG GCGCAGAGGT GACCCGACGG AGATCTTTTA ATGATTTCCG CTCCATCCTG 
CCCAGCTCAC TCCTCACCGT TTGCCAAAGT GACACTTTAC GCCGCAAAAC CAGCAGCACT 
TCCAGCATCT CACAACAGGC CAGTCCAGAA CGTGCTGGAC TTCCTCCGAG TTCTCCCTCT 
GACCCTTCTG CTCATTATTT CGAAGCTGCC TCGTATCTGC CCGACGGCAG TGCTGTGGAC 
CCGGACTACT ATTTCTCCAC CATCAGCTCC AGCTTTTCAG TCTCTCCGCT CTTCACAGGC 
AGCAACAATA AGGAGTTTAC CGTACCGCTG GATTTACTGA GGCAGCTTCT GCAGATGGTC 
GAGCAGTTTG TGTCAGAATC CTTCATGAAG ACACTGGATC AGGCCATGAC TGAAGTTCTG 
GAGATCAATC CCACGGCTTA TCTGTACTAC AAGACTTTCA GTGACCCGCT GTACGTGGCC 
ATCTTTAAAA TGCTGAGGGA CTCGTTGTAT TATATGAAAG ATCTGCAGGT GTCGTTTGTG 
AAGGAGGTCC ATGACTTTGT TCTGGAGCAG TTCAGCAGCA GTCAGTCTGA GCTGCAGAGG 
ATCCTGCACG ATGTGGAGTA TCTGCACAGT GAACTGAACC CGCTTAAGCT GCGCTGTCAG 
GCCAACGCCG CCTGTGTGGA CCTCATGGTG TGGGCCGTCA CGGAAGAACA GGGTGCTGAA 
AATCTCTGCA CTAAACTGTC AGAGAAGCTC CAGTCCAAGA CGTCCAGCAA AGTCATCATC 
GCCCACATGC CTCTGCTCAT CTGCTGCTTA CAGGGTCTCG GCCGGCTCTG TGAGCGTTTT 
CCTGTGGTGG CTCACTCTGT GACCATGTCT CTACGAGATT TTCTGGTGGT TCCTTCACCT 
GTACTAGTGA AGCTCTACAA GTACCACAGT CAGTACACCA CAGGAGCAGG TGAGATCAAG 
ATTCATGTCA CCAACGAGCA TTCCCAGTCC ACCTTCAGCT CGCACTCCAG CAAGAAGAGC 
CAGCCGTCCA TGTATGAGCA GCTCCGAGAC ATTTCCATCA ACAATATATG CAGGTGTTTA 
AAAGCAGGAT TGACCATGGA TTCAGTCATC GTTGAAGCTT TCCTCGCCAG TCTTTCCAAC 
CGTCTGTACA TTTCGCAGGA AAATGACAAG GATGCCCATC TAATCCCGGA CCACACCATA 
CGGGCGTTAG GACACATCGC TGTGGCTCTG AGAGACACTC CACGAGTGAT GGAGCCCATC 
TTACAGATCC TGCAGCAGAA GTTCTGCCAG CCGCCGTCTC AGCTCGACGT GCTCATCATA 
GATCAGCTGG GCTGTATGGT CATCACTGGA AACCAATATA TTTATCAGGA AGTGTGGAAC 
CTGTTCCAGC AGATCAGCGT GAAGGCCAGC TCCATGGTTT ACTCCACCAA AGACTACAAG 
GATCATGGAT ACAGGCACTG TTCTCTGGCC GTGATCAATG CTCTGGCCAA CATCGCAGCC 
AACCTGCAGA CGGAGCAGAT GGTGGACGAG CTGCTGGTCA ATCTGCTGGA ACTGTTTGTG 
CAGCTGGGGC TGGAGGGCAA ACGGGCCAGT GAAAGAGCCA GCGAGAAGGG CCCGGCCCTC 
AAGGCGTCAA GCAGCGCTGG TAATCTCGGA GTTCTCATTC CTGTCATTGC TGTGCTAACC 
AAACGTTTGC CGGCCATAAA AGAAGCCAAA CCGAGGCTGC AGAAGCTGTT CAGAGATTTC 
TGGCTGTATT CGGTTGTGAT GGGCTTCGCT GTTGAGGGCT CAGGTCTGTG GCCGGAGGAG 
TGGTATGAGG GAGTGTGTGA GATTGCAACA AAATCCCCAC TGCTGACCTT CCCAAGCGGA 
GAACCGCTGC GCTCAGAGCT GCAGTATAAC TCGGCCCTGA AGAACGACAC TGTCACGCCG 
GCTGAGCTGA ATGATTTGAG GGGAACCATC ATTAACCTGC TGGATCCTCC GCCGGAGGTT 
GCTGCGCTCA TTAATAAACT GGACTTTGCC ATGTCCACCT ACCTGCTGTC CGTCTACCGG 
CTGGAATACA TGAGAGTGTT ACGATCTCAG GAGTCTGACC GATTCCAAGT GATGTTTCGC 
TATTTTGAAG ACCGAGCGAT TCAGAAGGAT AAATCAGGAA TGATGCAGTG TGTCATCGCA 
GTAGGTGACA AGGTCTTCGA TGTCTTTCTT CAGATGATGG CTGACAAGGC AAAGACCAAA 
GCCCATGAGG AGGAGCTGGA AAGTCATGCT CAGTTTCTGT TGGTCAACTT CAACCACATT 
CATAAGAGAA TCCGAAGAGT TGCCGACAAA TATCTGTCAG GACTCGCCGA GACATTTCCA 
CACTTGCTGT GGAGCGGCCG TGTTCTGAGG ACCATGCTGG ATATATTACA GACTCTGTCT 
CTTTCGCTCA GTGCTGATAT CCACAAAGAC CAACCATATT ACGACATACC AGACACACCA 
TATCGCATCA CAGTGCCGGA CACGTATGAG GCCAGAGAGA GCATAGTGAA GGACTTTGCC 
GCCCGCTGTG GAGAAATCCT GAAAGAGGCC ATGAAATGGG CGCCATCGGT CACCAAATCA 
CACCTACAGG AATACCTGAA TAAGCATCAG AACTGGGTGT CGGGTCTGTC TCAGCACACA 
GGGCTGGCGA TGGCCACCGA GAGCATCCTG CACTTCGCCG GATACAACCG TCAGAGCACC 
AGTCTCGGTA CCACTCAGCT GACCGAGAGA CCCGCATGCG TGAAGAAAGA CTACTCCAAC 
TTCATGGCCT CCCTCAACCT GAGAAACCGC TACGCTGGAG AGGTCGCAGG GATGATCCAT 
TTCTCCGAGG CCACGCGGAG CACATCAGAC CTGAATAAAC TGATGATCCG ACAGATGAAT 
GAAGCTCTGG ATGGAGGACA GCCCGAAGCC TTCACCGAAG CCCTGTTCAA GATGGCAGCG 
CTGTTGATCA GCTCCAGAGA ATGGGACCCT CTGCTGTTGC ATCACCTGTG CTGGTCTCCA 
TTAAAGATGT TTACTGAGAA TGGCATGGAA ACGGCTATAG CCTGCTGGGA ATGGCTTCTG 
GCTGGAAGAA CAGGAGTTGA AGTCCCGTTC ATGCGTGAGA TGGCCGGCGC GTGGCAGATG 
ACCGTGGATC TGAAGATGGG GCTGTTTTCT GATGCCCAGC TAGAGGCCGA TCCCCTGGCG 
GCTTCCGAGG AAAGCCAACC CATCCCCTGC CCGCCCGACG TCACGCCTCA CTACATCTGG 
ATTGAGTTTC TGGTGCAGCG GTTTGAGATC GCCAAGTACA GCAGCGCCGA TCAGGTGGAG 
ATTTTCGGCA GTCTTCTGCA GAGGTCTCTC TCTCTGAATG TCGGCAGCCC AAAAAGCAGT 
CTGAACCGCC ATGTGGCAGC CATCGGACCT CGCTTCAGGT TGTTGACGCT CGGACTTTCC 
CTCCTGCATT CTGACGTAGT GACCAATGCC ACCATCCGTA ACGTCCTAAG GGAAAAGATT 
TATTCCACTG CCTTCGATTA CTTCAGTGTG GCATCAAAGT TCCCCACTCA GGGAGACAAG 
CGTCTGCGGG AGGACATCAG CATCATGATC CGCTTCTACG CCAGCATCCT CTCCGACAAG 
AAGTACCTAG GTGCTAGCCT GCTCGTGCCA CCTGACAACC AGGACCCGTC GCTGAACAGT 
CTATCAGTGA TGAACGCCAC CGACCCCAGG AACAACATGG ACATGACGGT GGGCTCCCGC 
CAGCAGAGCG CTCAGGGCTG GATCAACACG TACCCCCTGT CTAGCGGCAT GTCCACCACC 
TCCAAGAAAT CAGGCATGTC CAAAAAAAGC AACAGAGGAA CCCAGCTGCA CAAGTACTAC 
ATGAAACGCA GGACGTTGCT CTTGGCCTTG CTGGCCAGCG AGATCGAGAG ACTGAGCACA 
TGGTACAACC CGCTGTCCAC ACAGGAGCTG GCCATTCCCA CCGAGCAGTC CGTGGAAACC 
AGCATCTCCA ACTGGAGGTC CAAATACATC AACCTGACCG AGAAACAGTG GAAGGATAAT 
GTCAATTTGG CCTGGAGTAT CGCTCCTTAC CTGGCCTTGC AGCTTCCGGC AAGGTTCAAA 
AACGCGGACG CCATTGTGGC AGAAGTGACT CGTCTAGTTC GGCTGGATCC TGGTGCTGTC 
AGCGATGTGC CAGAGGCAGT GAAGTTCCTG GTAACGTGGC ACACCATTGA TGCAGATTCT 
CCAGAGTTGA GCCACATCTT GTGCTGGGCT CATGCAGATC CTCCGACCGG CCTCTCGTAT 
TTCTCCAGCA TGTATCCTCC TCATCCGCTC ACTGCTCAGT ATGGAGTCAA AGTCCTTCGC 
TCTTTTCCTC CTGATACCAT TTTGTTTTAC ATTCCTCAAA TCGTTCAGGC GCTGCGCTAC 
GACAAGATGG GTTACGTGCG CGAGTACATC CTGTGGGCCG CACAGAAGTC GCAGCTCCTC 
GCACATCAGT TCATATGGAA CATGAAGACC AACATCTTTC TGGATGAAGA GGGACATCAG 
AAAGACCCGG ACATCGGTGA GCTTCTGGAG CAGATGGTGG AAGAGATCAC GCACTCTCTG 
TCAGGTCCAG CTAAAGACTT CTATCAGCGC GAGTTTGACT TCTTCAACAA GATCACCAAC 
GTCTCCGCCA TCATCAAGCC TGTTCCCAAA GGAGATGAGA GGAAGAGAGC GTGTCTGAAG 
GCGCTGTCAG AAATCAAAGT TCAGCCAGGA TGTTATTTGC CAAGCAATCC AGAAGCAATC 
GTCCTTGATA TCGACTACAA GTCAGGAACG CCCATGCAGA GCGCTGCCAA AGCCCCTTAT 
CTGGCCAAAT TCAAAGTTAA GAGGTGTGGC GTTAGTGAGC TTGAAAAAGA GGGTCTGCGC 
TGTCCGTCAG ACTCTCAGGA TGAAGGAGAT GAGAGCTCAT TGATGGCTCA AAGGGTCTGC 
TGGCAGGCGG CCATCTTTAA AGTGGGCGAT GATTGCAGAC AGGACATGCT CGCGCTTCAG 
ATCATTGGCC TGTTCAAGAA CATCTTTCAG CTGGTGGGTT TGGATCTGTT TGTGTTCCCG 
TACCGGGTTG TGGCCACAGC TCCAGGATGT GGCGTGATTG AATGTATTCC GGACTGTAAA 
TCCCGGGATC AGCTCGGCCG GCAGACTGAC TTCGGCATGT ACGACTACTT CAGGAACCAA 
TATGGCGACG AATCCACTCT GGCGTTCCAG AAGGCTCGGT ATAACTTCAT TCGCAGTATG 
GCTGCTTACA GTCTGCTGCT CTTTCTGTTA CAAATCAAAG ACCGACACAA CGGCAACATT 
ATGCTGGACA GCAAAGGCCA TCTCATTCAC ATCGACTTTG GCTTCATGTT TGAGAGTTCT 
CCTGGTGGGA ATCTGGGCTG GGAGCCTGAC ATCAAGCTGA CCGATGAGAT GGTGATGATC 
ATGGGAGGCA AGATGGAGGC TACACCATTT AAATGGTTCA TGGAGATGTG TGTGCGCGGA 
TACCTGGCTG TAAGGCCCTA CATGGATGCT GTCGTGTCAT TGGTGACGTT GATGCTGGAC 
ACGGGGTTGC CTTGCTTCAG AGGACAGACG ATCAAACTCC TCAAGCAACG GTTCAACCCA 
AACATGAGCG AGAAGGAAGC TGCGGCCTTC ATGATCAAAG TCATCGAGCG CTGCTTCCTC 
AGTAGCAGGA GCAAAACCTA CGACATGCTG CAATACTACC AGAACCAAAT CCCATACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001030144.1
CDS	1..6180
Translation	MCPVDIRGVFQLDERRRDAVIALGIFLVESELQHKDAIVPYLLVLLKGLPRVQWIEESSERKGREALPIAENFSFCLVTVLSDVAQMDESMRIQILEAVMNLIQVLQETCQNIEGQDKEYLCKYTVPCLLGVVRAFGRYSNTEEPLLSKLFPRIVPQPPGTGDGAEVTRRRSFNDFRSILPSSLLTVCQSDTLRRKTSSTSSISQQASPERAGLPPSSPSDPSAHYFEAASYLPDGSAVDPDYYFSTISSSFSVSPLFTGSNNKEFTVPLDLLRQLLQMVEQFVSESFMKTLDQAMTEVLEINPTAYLYYKTFSDPLYVAIFKMLRDSLYYMKDLQVSFVKEVHDFVLEQFSSSQSELQRILHDVEYLHSELNPLKLRCQANAACVDLMVWAVTEEQGAENLCTKLSEKLQSKTSSKVIIAHMPLLICCLQGLGRLCERFPVVAHSVTMSLRDFLVVPSPVLVKLYKYHSQYTTGAGEIKIHVTNEHSQSTFSSHSSKKSQPSMYEQLRDISINNICRCLKAGLTMDSVIVEAFLASLSNRLYISQENDKDAHLIPDHTIRALGHIAVALRDTPRVMEPILQILQQKFCQPPSQLDVLIIDQLGCMVITGNQYIYQEVWNLFQQISVKASSMVYSTKDYKDHGYRHCSLAVINALANIAANLQTEQMVDELLVNLLELFVQLGLEGKRASERASEKGPALKASSSAGNLGVLIPVIAVLTKRLPAIKEAKPRLQKLFRDFWLYSVVMGFAVEGSGLWPEEWYEGVCEIATKSPLLTFPSGEPLRSELQYNSALKNDTVTPAELNDLRGTIINLLDPPPEVAALINKLDFAMSTYLLSVYRLEYMRVLRSQESDRFQVMFRYFEDRAIQKDKSGMMQCVIAVGDKVFDVFLQMMADKAKTKAHEEELESHAQFLLVNFNHIHKRIRRVADKYLSGLAETFPHLLWSGRVLRTMLDILQTLSLSLSADIHKDQPYYDIPDTPYRITVPDTYEARESIVKDFAARCGEILKEAMKWAPSVTKSHLQEYLNKHQNWVSGLSQHTGLAMATESILHFAGYNRQSTSLGTTQLTERPACVKKDYSNFMASLNLRNRYAGEVAGMIHFSEATRSTSDLNKLMIRQMNEALDGGQPEAFTEALFKMAALLISSREWDPLLLHHLCWSPLKMFTENGMETAIACWEWLLAGRTGVEVPFMREMAGAWQMTVDLKMGLFSDAQLEADPLAASEESQPIPCPPDVTPHYIWIEFLVQRFEIAKYSSADQVEIFGSLLQRSLSLNVGSPKSSLNRHVAAIGPRFRLLTLGLSLLHSDVVTNATIRNVLREKIYSTAFDYFSVASKFPTQGDKRLREDISIMIRFYASILSDKKYLGASLLVPPDNQDPSLNSLSVMNATDPRNNMDMTVGSRQQSAQGWINTYPLSSGMSTTSKKSGMSKKSNRGTQLHKYYMKRRTLLLALLASEIERLSTWYNPLSTQELAIPTEQSVETSISNWRSKYINLTEKQWKDNVNLAWSIAPYLALQLPARFKNADAIVAEVTRLVRLDPGAVSDVPEAVKFLVTWHTIDADSPELSHILCWAHADPPTGLSYFSSMYPPHPLTAQYGVKVLRSFPPDTILFYIPQIVQALRYDKMGYVREYILWAAQKSQLLAHQFIWNMKTNIFLDEEGHQKDPDIGELLEQMVEEITHSLSGPAKDFYQREFDFFNKITNVSAIIKPVPKGDERKRACLKALSEIKVQPGCYLPSNPEAIVLDIDYKSGTPMQSAAKAPYLAKFKVKRCGVSELEKEGLRCPSDSQDEGDESSLMAQRVCWQAAIFKVGDDCRQDMLALQIIGLFKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPDCKSRDQLGRQTDFGMYDYFRNQYGDESTLAFQKARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDSKGHLIHIDFGFMFESSPGGNLGWEPDIKLTDEMVMIMGGKMEATPFKWFMEMCVRGYLAVRPYMDAVVSLVTLMLDTGLPCFRGQTIKLLKQRFNPNMSEKEAAAFMIKVIERCFLSSRSKTYDMLQYYQNQIPY

Target ORF information:

RefSeq Version	NM_001034972.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphatidylinositol 4-kinase, catalytic, alpha a (pi4kaa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001034972.1

ORF Insert Sequence:

ATGTGTCCTG TGGACATCCG TGGTGTTTTC CAGCTGGACG AGCGGAGGAG AGATGCTGTG 
ATCGCACTTG GGATCTTCCT AGTGGAGTCT GAGTTGCAGC ACAAGGATGC TATTGTCCCG 
TACTTGTTGG TTTTGCTGAA GGGTTTGCCC AGAGTGCAAT GGATCGAGGA GAGCTCAGAA 
AGGAAAGGAC GAGAAGCGCT TCCCATTGCG GAGAATTTCT CCTTCTGTTT GGTGACGGTG 
CTCTCAGACG TGGCCCAGAT GGATGAATCC ATGCGCATCC AGATTTTGGA GGCTGTGATG 
AACCTCATTC AGGTGCTACA GGAAACATGT CAGAACATCG AGGGCCAAGA TAAAGAATAC 
CTGTGTAAAT ACACCGTGCC CTGCCTGCTC GGTGTGGTGC GAGCGTTTGG CCGCTACAGC 
AACACAGAGG AGCCCCTGTT GTCTAAACTC TTCCCCCGGA TCGTTCCTCA GCCTCCAGGC 
ACCGGCGACG GCGCAGAGGT GACCCGACGG AGATCTTTTA ATGATTTCCG CTCCATCCTG 
CCCAGCTCAC TCCTCACCGT TTGCCAAAGT GACACTTTAC GCCGCAAAAC CAGCAGCACT 
TCCAGCATCT CACAACAGGC CAGTCCAGAA CGTGCTGGAC TTCCTCCGAG TTCTCCCTCT 
GACCCTTCTG CTCATTATTT CGAAGCTGCC TCGTATCTGC CCGACGGCAG TGCTGTGGAC 
CCGGACTACT ATTTCTCCAC CATCAGCTCC AGCTTTTCAG TCTCTCCGCT CTTCACAGGC 
AGCAACAATA AGGAGTTTAC CGTACCGCTG GATTTACTGA GGCAGCTTCT GCAGATGGTC 
GAGCAGTTTG TGTCAGAATC CTTCATGAAG ACACTGGATC AGGCCATGAC TGAAGTTCTG 
GAGATCAATC CCACGGCTTA TCTGTACTAC AAGACTTTCA GTGACCCGCT GTACGTGGCC 
ATCTTTAAAA TGCTGAGGGA CTCGTTGTAT TATATGAAAG ATCTGCAGGT GTCGTTTGTG 
AAGGAGGTCC ATGACTTTGT TCTGGAGCAG TTCAGCAGCA GTCAGTCTGA GCTGCAGAGG 
ATCCTGCACG ATGTGGAGTA TCTGCACAGT GAACTGAACC CGCTTAAGCT GCGCTGTCAG 
GCCAACGCCG CCTGTGTGGA CCTCATGGTG TGGGCCGTCA CGGAAGAACA GGGTGCTGAA 
AATCTCTGCA CTAAACTGTC AGAGAAGCTC CAGTCCAAGA CGTCCAGCAA AGTCATCATC 
GCCCACATGC CTCTGCTCAT CTGCTGCTTA CAGGGTCTCG GCCGGCTCTG TGAGCGTTTT 
CCTGTGGTGG CTCACTCTGT GACCATGTCT CTACGAGATT TTCTGGTGGT TCCTTCACCT 
GTACTAGTGA AGCTCTACAA GTACCACAGT CAGTACACCA CAGGAGCAGG TGAGATCAAG 
ATTCATGTCA CCAACGAGCA TTCCCAGTCC ACCTTCAGCT CGCACTCCAG CAAGAAGAGC 
CAGCCGTCCA TGTATGAGCA GCTCCGAGAC ATTTCCATCA ACAATATATG CAGGTGTTTA 
AAAGCAGGAT TGACCATGGA TTCAGTCATC GTTGAAGCTT TCCTCGCCAG TCTTTCCAAC 
CGTCTGTACA TTTCGCAGGA AAATGACAAG GATGCCCATC TAATCCCGGA CCACACCATA 
CGGGCGTTAG GACACATCGC TGTGGCTCTG AGAGACACTC CACGAGTGAT GGAGCCCATC 
TTACAGATCC TGCAGCAGAA GTTCTGCCAG CCGCCGTCTC AGCTCGACGT GCTCATCATA 
GATCAGCTGG GCTGTATGGT CATCACTGGA AACCAATATA TTTATCAGGA AGTGTGGAAC 
CTGTTCCAGC AGATCAGCGT GAAGGCCAGC TCCATGGTTT ACTCCACCAA AGACTACAAG 
GATCATGGAT ACAGGCACTG TTCTCTGGCC GTGATCAATG CTCTGGCCAA CATCGCAGCC 
AACCTGCAGA CGGAGCAGAT GGTGGACGAG CTGCTGGTCA ATCTGCTGGA ACTGTTTGTG 
CAGCTGGGGC TGGAGGGCAA ACGGGCCAGT GAAAGAGCCA GCGAGAAGGG CCCGGCCCTC 
AAGGCGTCAA GCAGCGCTGG TAATCTCGGA GTTCTCATTC CTGTCATTGC TGTGCTAACC 
AAACGTTTGC CGGCCATAAA AGAAGCCAAA CCGAGGCTGC AGAAGCTGTT CAGAGATTTC 
TGGCTGTATT CGGTTGTGAT GGGCTTCGCT GTTGAGGGCT CAGGTCTGTG GCCGGAGGAG 
TGGTATGAGG GAGTGTGTGA GATTGCAACA AAATCCCCAC TGCTGACCTT CCCAAGCGGA 
GAACCGCTGC GCTCAGAGCT GCAGTATAAC TCGGCCCTGA AGAACGACAC TGTCACGCCG 
GCTGAGCTGA ATGATTTGAG GGGAACCATC ATTAACCTGC TGGATCCTCC GCCGGAGGTT 
GCTGCGCTCA TTAATAAACT GGACTTTGCC ATGTCCACCT ACCTGCTGTC CGTCTACCGG 
CTGGAATACA TGAGAGTGTT ACGATCTCAG GAGTCTGACC GATTCCAAGT GATGTTTCGC 
TATTTTGAAG ACCGAGCGAT TCAGAAGGAT AAATCAGGAA TGATGCAGTG TGTCATCGCA 
GTAGGTGACA AGGTCTTCGA TGTCTTTCTT CAGATGATGG CTGACAAGGC AAAGACCAAA 
GCCCATGAGG AGGAGCTGGA AAGTCATGCT CAGTTTCTGT TGGTCAACTT CAACCACATT 
CATAAGAGAA TCCGAAGAGT TGCCGACAAA TATCTGTCAG GACTCGCCGA GACATTTCCA 
CACTTGCTGT GGAGCGGCCG TGTTCTGAGG ACCATGCTGG ATATATTACA GACTCTGTCT 
CTTTCGCTCA GTGCTGATAT CCACAAAGAC CAACCATATT ACGACATACC AGACACACCA 
TATCGCATCA CAGTGCCGGA CACGTATGAG GCCAGAGAGA GCATAGTGAA GGACTTTGCC 
GCCCGCTGTG GAGAAATCCT GAAAGAGGCC ATGAAATGGG CGCCATCGGT CACCAAATCA 
CACCTACAGG AATACCTGAA TAAGCATCAG AACTGGGTGT CGGGTCTGTC TCAGCACACA 
GGGCTGGCGA TGGCCACCGA GAGCATCCTG CACTTCGCCG GATACAACCG TCAGAGCACC 
AGTCTCGGTA CCACTCAGCT GACCGAGAGA CCCGCATGCG TGAAGAAAGA CTACTCCAAC 
TTCATGGCCT CCCTCAACCT GAGAAACCGC TACGCTGGAG AGGTCGCAGG GATGATCCAT 
TTCTCCGAGG CCACGCGGAG CACATCAGAC CTGAATAAAC TGATGATCCG ACAGATGAAT 
GAAGCTCTGG ATGGAGGACA GCCCGAAGCC TTCACCGAAG CCCTGTTCAA GATGGCAGCG 
CTGTTGATCA GCTCCAGAGA ATGGGACCCT CTGCTGTTGC ATCACCTGTG CTGGTCTCCA 
TTAAAGATGT TTACTGAGAA TGGCATGGAA ACGGCTATAG CCTGCTGGGA ATGGCTTCTG 
GCTGGAAGAA CAGGAGTTGA AGTCCCGTTC ATGCGTGAGA TGGCCGGCGC GTGGCAGATG 
ACCGTGGATC TGAAGATGGG GCTGTTTTCT GATGCCCAGC TAGAGGCCGA TCCCCTGGCG 
GCTTCCGAGG AAAGCCAACC CATCCCCTGC CCGCCCGACG TCACGCCTCA CTACATCTGG 
ATTGAGTTTC TGGTGCAGCG GTTTGAGATC GCCAAGTACA GCAGCGCCGA TCAGGTGGAG 
ATTTTCGGCA GTCTTCTGCA GAGGTCTCTC TCTCTGAATG TCGGCAGCCC AAAAAGCAGT 
CTGAACCGCC ATGTGGCAGC CATCGGACCT CGCTTCAGGT TGTTGACGCT CGGACTTTCC 
CTCCTGCATT CTGACGTAGT GACCAATGCC ACCATCCGTA ACGTCCTAAG GGAAAAGATT 
TATTCCACTG CCTTCGATTA CTTCAGTGTG GCATCAAAGT TCCCCACTCA GGGAGACAAG 
CGTCTGCGGG AGGACATCAG CATCATGATC CGCTTCTACG CCAGCATCCT CTCCGACAAG 
AAGTACCTAG GTGCTAGCCT GCTCGTGCCA CCTGACAACC AGGACCCGTC GCTGAACAGT 
CTATCAGTGA TGAACGCCAC CGACCCCAGG AACAACATGG ACATGACGGT GGGCTCCCGC 
CAGCAGAGCG CTCAGGGCTG GATCAACACG TACCCCCTGT CTAGCGGCAT GTCCACCACC 
TCCAAGAAAT CAGGCATGTC CAAAAAAAGC AACAGAGGAA CCCAGCTGCA CAAGTACTAC 
ATGAAACGCA GGACGTTGCT CTTGGCCTTG CTGGCCAGCG AGATCGAGAG ACTGAGCACA 
TGGTACAACC CGCTGTCCAC ACAGGAGCTG GCCATTCCCA CCGAGCAGTC CGTGGAAACC 
AGCATCTCCA ACTGGAGGTC CAAATACATC AACCTGACCG AGAAACAGTG GAAGGATAAT 
GTCAATTTGG CCTGGAGTAT CGCTCCTTAC CTGGCCTTGC AGCTTCCGGC AAGGTTCAAA 
AACGCGGACG CCATTGTGGC AGAAGTGACT CGTCTAGTTC GGCTGGATCC TGGTGCTGTC 
AGCGATGTGC CAGAGGCAGT GAAGTTCCTG GTAACGTGGC ACACCATTGA TGCAGATTCT 
CCAGAGTTGA GCCACATCTT GTGCTGGGCT CATGCAGATC CTCCGACCGG CCTCTCGTAT 
TTCTCCAGCA TGTATCCTCC TCATCCGCTC ACTGCTCAGT ATGGAGTCAA AGTCCTTCGC 
TCTTTTCCTC CTGATACCAT TTTGTTTTAC ATTCCTCAAA TCGTTCAGGC GCTGCGCTAC 
GACAAGATGG GTTACGTGCG CGAGTACATC CTGTGGGCCG CACAGAAGTC GCAGCTCCTC 
GCACATCAGT TCATATGGAA CATGAAGACC AACATCTTTC TGGATGAAGA GGGACATCAG 
AAAGACCCGG ACATCGGTGA GCTTCTGGAG CAGATGGTGG AAGAGATCAC GCACTCTCTG 
TCAGGTCCAG CTAAAGACTT CTATCAGCGC GAGTTTGACT TCTTCAACAA GATCACCAAC 
GTCTCCGCCA TCATCAAGCC TGTTCCCAAA GGAGATGAGA GGAAGAGAGC GTGTCTGAAG 
GCGCTGTCAG AAATCAAAGT TCAGCCAGGA TGTTATTTGC CAAGCAATCC AGAAGCAATC 
GTCCTTGATA TCGACTACAA GTCAGGAACG CCCATGCAGA GCGCTGCCAA AGCCCCTTAT 
CTGGCCAAAT TCAAAGTTAA GAGGTGTGGC GTTAGTGAGC TTGAAAAAGA GGGTCTGCGC 
TGTCCGTCAG ACTCTCAGGA TGAAGGAGAT GAGAGCTCAT TGATGGCTCA AAGGGTCTGC 
TGGCAGGCGG CCATCTTTAA AGTGGGCGAT GATTGCAGAC AGGACATGCT CGCGCTTCAG 
ATCATTGGCC TGTTCAAGAA CATCTTTCAG CTGGTGGGTT TGGATCTGTT TGTGTTCCCG 
TACCGGGTTG TGGCCACAGC TCCAGGATGT GGCGTGATTG AATGTATTCC GGACTGTAAA 
TCCCGGGATC AGCTCGGCCG GCAGACTGAC TTCGGCATGT ACGACTACTT CAGGAACCAA 
TATGGCGACG AATCCACTCT GGCGTTCCAG AAGGCTCGGT ATAACTTCAT TCGCAGTATG 
GCTGCTTACA GTCTGCTGCT CTTTCTGTTA CAAATCAAAG ACCGACACAA CGGCAACATT 
ATGCTGGACA GCAAAGGCCA TCTCATTCAC ATCGACTTTG GCTTCATGTT TGAGAGTTCT 
CCTGGTGGGA ATCTGGGCTG GGAGCCTGAC ATCAAGCTGA CCGATGAGAT GGTGATGATC 
ATGGGAGGCA AGATGGAGGC TACACCATTT AAATGGTTCA TGGAGATGTG TGTGCGCGGA 
TACCTGGCTG TAAGGCCCTA CATGGATGCT GTCGTGTCAT TGGTGACGTT GATGCTGGAC 
ACGGGGTTGC CTTGCTTCAG AGGACAGACG ATCAAACTCC TCAAGCAACG GTTCAACCCA 
AACATGAGCG AGAAGGAAGC TGCGGCCTTC ATGATCAAAG TCATCGAGCG CTGCTTCCTC 
AGTAGCAGGA GCAAAACCTA CGACATGCTG CAATACTACC AGAACCAAAT CCCATACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pi4kaa ( XM_005172596.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172653.1 Danio rerio phosphatidylinositol 4-kinase, catalytic, alpha a (pi4kaa), transcript variant X2, mRNA.

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CloneID	ODa15706
Clone ID Related Accession (Same CDS sequence)	XM_005172596.3 , XM_005172596.4
Accession Version	XM_005172596.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	phosphatidylinositol 4-kinase alpha isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172596.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCGA GAGGGACGTC GCGGGGTTTT TACTTTAACA CTGTGCTTTC TCTCGCGCGC 
TCACTGGCTG CTCATCGACC CGCTCCGATC GACAAGGTCC AGAAGCTACA ATGCATGTGT 
CCTGTGGACA TCCGTGGTGT TTTCCAGCTG GACGAGCGGA GGAGAGATGC TGTGATCGCA 
CTTGGGATCT TCCTAGTGGA GTCTGAGTTG CAGCACAAGG ATGCTATTGT CCCGTACTTG 
TTGGTTTTGC TAAAGGGATT GCCCAGGGTG CAATGGATCG AGGAGAGCTC AGAAAGGAAA 
GGACGAGAAG CGCTTCCCAT TGCGGAGAAT TTCTCCTTCT GTTTGGTGAC GGTGCTCTCA 
GACGTGGCCC AGATGGATGA ATCCATGCGC ATCCAGATTT TGGAGGCTGT GATGAACCTC 
ATTCAGGTGC TACAGGAAAC ATGTCAGAAC ATCGAGGGCC AAGATAAAGA ATACCTGTGT 
AAATACACCG TGCCCTGTCT GCTCGGTGTG GTGCGAGCGT TTGGCCGCTA CAGCAACACA 
GAGGAGCCGC TGTTGTCTAA ACTCTTCCCC CGGATCGTTC CTCAGCCTCC AGGCACCGGC 
GACGGCGCAG AGGTGACCCG ACGGAGATCT TTTAATGATT TCCGCTCCAT CCTGCCCAGC 
TCACTCCTCA CCGTTTGCCA AAGTGACACT TTACGCCGCA AAACCAGCAG CACTTCCAGC 
ATCTCACAAC AGGCCAGTCC AGAACGTGCT GGACTTCCTC CGAGTTCTCC CTCTGACCCT 
TCTGCTCCTT ATTTCGAAGC TGCCTCGTAT CTGCCCGACG GCAGTGCTGT GGACCCGGAC 
TACTATTTCT CCACCATCAG CTCCAGCTTC TCAGTCTCTC CGCTCTTCAC AGGCAGCAAC 
AATAAAGAGT TTACCGTACC GCTGGATTTA CTGAGGCAGC TTCTGCAGAT GGTCGAGCAG 
TTTGTGTCAG AATCCTTCAT GAAGACACTG GATCAGGCCA TGACTGAAGT TCTGGAGATC 
AATCCCACGG CTTATCTGTA CTACAAGACT TTCAGTGACC CGCTGTACGT GGCCATCTTT 
AAAATGCTGA GGGACTCGTT GTATTATATG AAAGATCTGC AGGTGTCGTT TGTGAAGGAG 
GTCCATGACT TTGTTCTGGA GCAGTTCAGC AGCAGTCAGT CTGAGCTGCA GAGGATCCTG 
CACGATGTGG AGTATCTGCA CAGTGAACTG AACCCGCTTA AGCTGCGCTG TCAGGCCAAC 
GCCGCCTGTG TGGACCTCAT GGTGTGGGCC GTCACGGAAG AACAGGGCGC TGAAAATCTC 
TGCACTAAAC TGTCAGAGAA GCTCCAGTCC AAGACGTCCA GCAAAGTCAT CATCGCCCAC 
ATGCCTCTGC TCATCTGCTG CTTACAGGGT CTCGGCCGGC TCTGTGAGCG TTTTCCTGTG 
GTGGCTCACT CTGTGACCAT GTCTCTACGA GATTTTCTGG TGGTTCCTTC ACCTGTACTA 
GTGAAGCTCT ACAAGTACCA CAGTCAGTAC ACCACAGGAG CAGGTGAGAT CAAGATTCAT 
GTCACCAACG AGCATTCCCA GTCCACCTTC AGCTCGCACT CCAGCAAGAA GAGCCAGCCG 
TCCATGTATG AGCAGCTCCG AGACATTTCC ATCAACAATA TATGCAGGTG TTTAAAAGCA 
GGATTGACCA TGGATTCAGT CATCGTTGAA GCTTTCCTCG CCAGTCTTTC CAACCGTCTG 
TACATTTCGC AGGAAAATGA CAAGGATGCC CATCTAATCC CGGACCACAC CATACGGGCG 
TTAGGACACA TCGCTGTGGC TCTGAGAGAC ACTCCACGAG TGATGGAGCC CATCTTACAG 
ATCCTGCAGC AGAAGTTCTG CCAGCCGCCG TCTCAGCTCG ACGTGCTCAT CATAGATCAG 
CTGGGCTGTA TGGTCATCAC TGGAAACCAA TATATTTATC AGGAAGTGTG GAACCTGTTC 
CAGCAGATCA GCGTGAAGGC CAGCTCCATG GTTTACTCCA CCAAAGACTA CAAGGATCAT 
GGATACAGGC ACTGTTCTCT GGCCGTGATC AATGCTCTGG CCAACATCGC AGCCAACCTG 
CAGACGGAGC AGATGGTGGA CGAGCTGCTG GTCAATCTGC TGGAACTGTT TGTGCAGCTG 
GGGCTGGAGG GCAAACGGGC CAGTGAAAGA GCCAGCGAGA AGGGCCCGGC CCTCAAGGCG 
TCAAGCAGCG CTGGTAATCT CGGAGTTCTC ATTCCTGTCA TTGCTGTGCT AACCAAACGT 
TTGCCGGCCA TAAAAGAAGC CAAACCGAGG CTGCAGAAGC TGTTCAGAGA TTTCTGGCTG 
TATTCGGTTG TGATGGGCTT CGCTGTTGAG GGCTCAGGTC TGTGGCCGGA GGAGTGGTAT 
GAGGGAGTGT GTGAGATTGC AACAAAATCC CCACTGCTGA CCTTCCCAAG CGGAGAACCG 
CTGCGCTCAG AGCTGCAGTA TAACTCGGCC CTGAAGAACG ACACTGTCAC GCCGGCTGAG 
CTGAATGATT TGAGGGGAAC CATCATTAAC CTGCTGGATC CTCCGCCGGA GGTTGCTGCG 
CTCATTAATA AACTGGACTT TGCCATGTCC ACCTACCTGC TGTCCGTCTA CCGGCTGGAA 
TACATGAGAG TGTTACGATC TCAGGAGTCT GACCGATTCC AAGTGATGTT TCGCTATTTT 
GAAGACCGAG CGATTCAGAA GGATAAATCA GGAATGATGC AGTGTGTCAT CGCAGTAGGT 
GACAAGGTCT TCGATGTCTT TCTTCAGATG ATGGCTGACA AGGCAAAGAC CAAAGCCCAT 
GAGGAGGAGC TGGAAAGTCA TGCTCAGTTT CTATTGGTCA ACTTCAACCA CATTCATAAG 
AGAATCCGAA GAGTTGCTGA CAAATATCTG TCAGGACTCG CTGAGACATT TCCACACTTG 
CTGTGGAGCG GCCGTGTTCT GAGGACCATG CTGGATATAT TACAGACTCT GTCTCTTTCG 
CTCAGTGCTG ATATCCACAA AGACCAACCA TATTACGACA TACCAGACAC ACCATATCGC 
ATCACAGTGC CGGACACGTA TGAGGCCAGA GAGAGCATAG TGAAGGACTT TGCCGCCCGC 
TGTGGAGAAA TCCTGAAAGA GGCCATGAAA TGGGCGCCAT CGGTCACCAA ATCACACCTA 
CAGGAATACC TGAATAAGCA TCAGAACTGG GTGTCGGGTC TGTCTCAGCA CACAGGGCTG 
GCGATGGCCA CAGAGAGCAT CCTGCACTTC GCCGGATACA ACCGTCAGAG CACCAGTCTC 
GGTACCACTC AGCTGACCGA GAGACCCGCA TGCGTGAAGA AAGACTACTC CAACTTCATG 
GCCTCCCTCA ACCTGAGAAA CCGCTACGCT GGAGAGGTCG CAGGGATGAT CCATTTCTCC 
GAGGCCACGC GGAGCACATC AGACCTGAAT AAACTGATGA TCCGACAGAT GAATGAAGCT 
CTGGATGGAG GACAGCCCGA AGCCTTCACC GAAGCCCTGT TCAAGATGGC AGCGCTGTTG 
ATCAGCTCCA GAGAATGGGA CCCTCTGCTG TTGCATCACC TGTGCTGGTC TCCATTAAAG 
ATGTTTACTG AGAATGGCAT GGAAACGGCT ATAGCCTGCT GGGAATGGCT TCTGGCTGGA 
AGAACAGGAG TTGAAGTCCC GTTCATGCGT GAGATGGCCG GCGCGTGGCA GATGACGGTG 
GATCTGAAGA TGGGGCTGTT TTCTGATGCC CAGCTGGAGG CCGATCCCCT GGCGGCTTCC 
GAGGAAAGCC AACCCATCCC CTGCCCGCCC GACGTCACGC CACACTACAT CTGGATTGAG 
TTTCTGGTGC AGCGGTTTGA GATCGCCAAG TACAGCAGCG CCGATCAGGT GGAGATTTTC 
GGCAGTCTTC TGCAGAGGTC CCTCTCTCTG AATGTCGGCA GCCCAAAAAG CAGTCTGAAC 
CGCCATGTGG CAGCCATCGG ACCTCGCTTC AGGTTGTTGA CGCTCGGACT TTCTCTCCTG 
CATTCTGACG TAGTGACCAA TGCCACCATC CGTAACGTCC TAAGGGAAAA GATTTATTCC 
ACTGCCTTCG ATTACTTCAG TGTGGCATCA AAGTTCCCCA CTCAGGGAGA CAAGCGTCTG 
CGGGAGGACA TCAGCATCAT GATCCGCTTC TACGCCAGCA TCCTCTCCGA CAAGAAGTAC 
CTAGGTGCTA GCCTGCTCGT GCCACCTGAC AACCAGGACC CGTCGCTGAA CAGTCTATCA 
GTGATGAACG CCACCGACCC CAGGAACAAC ATGGACATGA CGGTGGGCTC CCGCCAGCAG 
AGCGCTCAGG GCTGGATCAA CACGTACCCC CTGTCTAGCG GCATGTCCAC CACCTCTAAG 
AAATCAGGCA TGTCCAAAAA AAGCAACAGA GGAACCCAGC TGCACAAGTA CTACATGAAA 
CGCAGGACGT TGCTCTTGGC CTTGCTGGCC AGCGAGATCG AGAGACTGAG CACATGGTAC 
AACCCGCTGT CCACACAGGA GCTGGCCATT CCCACCGAGC AGTCTGTGGA AACCAGCATC 
TCCAACTGGA GGTCCAAATA CATCAACCTG ACCGAGAAAC AGTGGAAGGA TAATGTCAAT 
TTGGCCTGGA GTATCGCTCC TTACCTGGCC TTGCAGCTTC CGGCAAGGTT CAAAAACGCA 
GACGCCATTG TGGCAGAAGT GACTCGTCTA GTTCGGCTGG ATCCTGGTGC TGTCAGCGAT 
GTGCCAGAGG CAGTGAAGTT CCTGGTAACG TGGCACACCA TTGATGCAGA TTCTCCAGAG 
CTGAGCCACA TCTTGTGCTG GGCTCCTGCA GATCCTCCGA CCGGCCTCTC GTATTTCTCC 
AGCATGTATC CTCCTCATCC GCTCACTGCT CAGTATGGAG TCAAAGTCCT TCGCTCTTTT 
CCTCCTGATG CCATTTTGTT TTACATTCCT CAAATCGTTC AGGCGCTGCG CTACGACAAG 
ATGGGTTACG TGCGCGAGTA CATCCTGTGG GCCGCACAGA AGTCGCAGCT CCTCGCACAT 
CAGTTCATAT GGAACATGAA GACCAACATC TTTCTGGATG AAGAGGGACA TCAGAAAGAC 
CCGGACATCG GTGAGCTTCT GGAGCAGATG GTGGAAGAGA TCACGCACTC TCTGTCAGGT 
CCAGCTAAAG ACTTCTATCA GCGCGAGTTT GACTTCTTCA ACAAGATCAC CAACGTCTCC 
GCCATCATCA AGCCTGTTCC CAAAGGAGAT GAGAGGAAGA GAGCGTGTCT GAAGGCGCTG 
TCAGAAATCA AAGTTCAGCC AGGATGTTAT TTGCCAAGCA ATCCAGAAGC AATCGTCCTT 
GATATCGACT ACAAGTCAGG AACGCCCATG CAGAGCGCTG CCAAAGCCCC TTATCTGGCC 
AAATTCAAAG TTAAGAGGTG TGGCGTTAGT GAGCTTGAAA AAGAGGGTCT GCGCTGTCCG 
TCCGACTCTC AGGATGAAGG AGATGAGAGC TCACTGACGG CTCAAAGGGT CTGCTGGCAG 
GCGGCCATCT TTAAAGTGGG CGATGATTGC AGACAGGACA TGCTCGCGCT TCAGATCATT 
GGCCTGTTCA AGAACATCTT TCAGCTGGTG GGTTTGGATC TGTTTGTGTT CCCGTACCGG 
GTTGTGGCCA CAGCTCCAGG ATGTGGCGTG ATTGAATGTA TTCCGGACTG TAAATCCCGG 
GATCAGCTCG GCCGGCAGAC TGACTTCGGC ATGTACGACT ACTTCAGGAA CCAATATGGC 
GACGAATCCA CTCTGGCTTT CCAGAAGGCT CGGTATAACT TCATTCGCAG TATGGCTGCT 
TACAGTCTGC TGCTCTTTCT GTTACAAATC AAAGACCGAC ACAACGGCAA CATCATGCTG 
GACAGCAAAG GCCATCTCAT TCACATCGAC TTTGGCTTCA TGTTTGAGAG TTCTCCTGGT 
GGGAATCTGG GCTGGGAGCC TGACATCAAG CTGACCGATG AGATGGTGAT GATCATGGGA 
GGCAAGATGG AGGCTACACC ATTTAAATGG TTCATGGAGA TGTGTGTGCG CGGATACCTG 
GCTGTAAGGC CCTACATGGA TGCTGTCGTG TCATTGGTGA CGTTGATGCT GGACACGGGG 
TTGCCTTGCT TCAGAGGACA GACGATCAAA CTCCTCAAGC AACGGTTCAA CCCAAACATG 
AGCGAGAAGG AAGCTGCGGC CTTCATGATC AAAGTCATCG AGCGCTGCTT CCTCAGTAGC 
AGGAGCAAAA CCTACGACAT GCTGCAATAC TACCAGAACC AAATCCCATA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172653.1
CDS	99..6392
Translation	MSSRGTSRGFYFNTVLSLARSLAAHRPAPIDKVQKLQCMCPVDIRGVFQLDERRRDAVIALGIFLVESELQHKDAIVPYLLVLLKGLPRVQWIEESSERKGREALPIAENFSFCLVTVLSDVAQMDESMRIQILEAVMNLIQVLQETCQNIEGQDKEYLCKYTVPCLLGVVRAFGRYSNTEEPLLSKLFPRIVPQPPGTGDGAEVTRRRSFNDFRSILPSSLLTVCQSDTLRRKTSSTSSISQQASPERAGLPPSSPSDPSAPYFEAASYLPDGSAVDPDYYFSTISSSFSVSPLFTGSNNKEFTVPLDLLRQLLQMVEQFVSESFMKTLDQAMTEVLEINPTAYLYYKTFSDPLYVAIFKMLRDSLYYMKDLQVSFVKEVHDFVLEQFSSSQSELQRILHDVEYLHSELNPLKLRCQANAACVDLMVWAVTEEQGAENLCTKLSEKLQSKTSSKVIIAHMPLLICCLQGLGRLCERFPVVAHSVTMSLRDFLVVPSPVLVKLYKYHSQYTTGAGEIKIHVTNEHSQSTFSSHSSKKSQPSMYEQLRDISINNICRCLKAGLTMDSVIVEAFLASLSNRLYISQENDKDAHLIPDHTIRALGHIAVALRDTPRVMEPILQILQQKFCQPPSQLDVLIIDQLGCMVITGNQYIYQEVWNLFQQISVKASSMVYSTKDYKDHGYRHCSLAVINALANIAANLQTEQMVDELLVNLLELFVQLGLEGKRASERASEKGPALKASSSAGNLGVLIPVIAVLTKRLPAIKEAKPRLQKLFRDFWLYSVVMGFAVEGSGLWPEEWYEGVCEIATKSPLLTFPSGEPLRSELQYNSALKNDTVTPAELNDLRGTIINLLDPPPEVAALINKLDFAMSTYLLSVYRLEYMRVLRSQESDRFQVMFRYFEDRAIQKDKSGMMQCVIAVGDKVFDVFLQMMADKAKTKAHEEELESHAQFLLVNFNHIHKRIRRVADKYLSGLAETFPHLLWSGRVLRTMLDILQTLSLSLSADIHKDQPYYDIPDTPYRITVPDTYEARESIVKDFAARCGEILKEAMKWAPSVTKSHLQEYLNKHQNWVSGLSQHTGLAMATESILHFAGYNRQSTSLGTTQLTERPACVKKDYSNFMASLNLRNRYAGEVAGMIHFSEATRSTSDLNKLMIRQMNEALDGGQPEAFTEALFKMAALLISSREWDPLLLHHLCWSPLKMFTENGMETAIACWEWLLAGRTGVEVPFMREMAGAWQMTVDLKMGLFSDAQLEADPLAASEESQPIPCPPDVTPHYIWIEFLVQRFEIAKYSSADQVEIFGSLLQRSLSLNVGSPKSSLNRHVAAIGPRFRLLTLGLSLLHSDVVTNATIRNVLREKIYSTAFDYFSVASKFPTQGDKRLREDISIMIRFYASILSDKKYLGASLLVPPDNQDPSLNSLSVMNATDPRNNMDMTVGSRQQSAQGWINTYPLSSGMSTTSKKSGMSKKSNRGTQLHKYYMKRRTLLLALLASEIERLSTWYNPLSTQELAIPTEQSVETSISNWRSKYINLTEKQWKDNVNLAWSIAPYLALQLPARFKNADAIVAEVTRLVRLDPGAVSDVPEAVKFLVTWHTIDADSPELSHILCWAPADPPTGLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGYVREYILWAAQKSQLLAHQFIWNMKTNIFLDEEGHQKDPDIGELLEQMVEEITHSLSGPAKDFYQREFDFFNKITNVSAIIKPVPKGDERKRACLKALSEIKVQPGCYLPSNPEAIVLDIDYKSGTPMQSAAKAPYLAKFKVKRCGVSELEKEGLRCPSDSQDEGDESSLTAQRVCWQAAIFKVGDDCRQDMLALQIIGLFKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPDCKSRDQLGRQTDFGMYDYFRNQYGDESTLAFQKARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDSKGHLIHIDFGFMFESSPGGNLGWEPDIKLTDEMVMIMGGKMEATPFKWFMEMCVRGYLAVRPYMDAVVSLVTLMLDTGLPCFRGQTIKLLKQRFNPNMSEKEAAAFMIKVIERCFLSSRSKTYDMLQYYQNQIPY

Target ORF information:

RefSeq Version	XM_005172596.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio phosphatidylinositol 4-kinase, catalytic, alpha a (pi4kaa), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172596.3

ORF Insert Sequence:

ATGTCGTCGA GAGGGACGTC GCGGGGTTTT TACTTTAACA CTGTGCTTTC TCTCGCGCGC 
TCACTGGCTG CTCATCGACC CGCTCCGATC GACAAGGTCC AGAAGCTACA ATGCATGTGT 
CCTGTGGACA TCCGTGGTGT TTTCCAGCTG GACGAGCGGA GGAGAGATGC TGTGATCGCA 
CTTGGGATCT TCCTAGTGGA GTCTGAGTTG CAGCACAAGG ATGCTATTGT CCCGTACTTG 
TTGGTTTTGC TAAAGGGATT GCCCAGGGTG CAATGGATCG AGGAGAGCTC AGAAAGGAAA 
GGACGAGAAG CGCTTCCCAT TGCGGAGAAT TTCTCCTTCT GTTTGGTGAC GGTGCTCTCA 
GACGTGGCCC AGATGGATGA ATCCATGCGC ATCCAGATTT TGGAGGCTGT GATGAACCTC 
ATTCAGGTGC TACAGGAAAC ATGTCAGAAC ATCGAGGGCC AAGATAAAGA ATACCTGTGT 
AAATACACCG TGCCCTGTCT GCTCGGTGTG GTGCGAGCGT TTGGCCGCTA CAGCAACACA 
GAGGAGCCGC TGTTGTCTAA ACTCTTCCCC CGGATCGTTC CTCAGCCTCC AGGCACCGGC 
GACGGCGCAG AGGTGACCCG ACGGAGATCT TTTAATGATT TCCGCTCCAT CCTGCCCAGC 
TCACTCCTCA CCGTTTGCCA AAGTGACACT TTACGCCGCA AAACCAGCAG CACTTCCAGC 
ATCTCACAAC AGGCCAGTCC AGAACGTGCT GGACTTCCTC CGAGTTCTCC CTCTGACCCT 
TCTGCTCCTT ATTTCGAAGC TGCCTCGTAT CTGCCCGACG GCAGTGCTGT GGACCCGGAC 
TACTATTTCT CCACCATCAG CTCCAGCTTC TCAGTCTCTC CGCTCTTCAC AGGCAGCAAC 
AATAAAGAGT TTACCGTACC GCTGGATTTA CTGAGGCAGC TTCTGCAGAT GGTCGAGCAG 
TTTGTGTCAG AATCCTTCAT GAAGACACTG GATCAGGCCA TGACTGAAGT TCTGGAGATC 
AATCCCACGG CTTATCTGTA CTACAAGACT TTCAGTGACC CGCTGTACGT GGCCATCTTT 
AAAATGCTGA GGGACTCGTT GTATTATATG AAAGATCTGC AGGTGTCGTT TGTGAAGGAG 
GTCCATGACT TTGTTCTGGA GCAGTTCAGC AGCAGTCAGT CTGAGCTGCA GAGGATCCTG 
CACGATGTGG AGTATCTGCA CAGTGAACTG AACCCGCTTA AGCTGCGCTG TCAGGCCAAC 
GCCGCCTGTG TGGACCTCAT GGTGTGGGCC GTCACGGAAG AACAGGGCGC TGAAAATCTC 
TGCACTAAAC TGTCAGAGAA GCTCCAGTCC AAGACGTCCA GCAAAGTCAT CATCGCCCAC 
ATGCCTCTGC TCATCTGCTG CTTACAGGGT CTCGGCCGGC TCTGTGAGCG TTTTCCTGTG 
GTGGCTCACT CTGTGACCAT GTCTCTACGA GATTTTCTGG TGGTTCCTTC ACCTGTACTA 
GTGAAGCTCT ACAAGTACCA CAGTCAGTAC ACCACAGGAG CAGGTGAGAT CAAGATTCAT 
GTCACCAACG AGCATTCCCA GTCCACCTTC AGCTCGCACT CCAGCAAGAA GAGCCAGCCG 
TCCATGTATG AGCAGCTCCG AGACATTTCC ATCAACAATA TATGCAGGTG TTTAAAAGCA 
GGATTGACCA TGGATTCAGT CATCGTTGAA GCTTTCCTCG CCAGTCTTTC CAACCGTCTG 
TACATTTCGC AGGAAAATGA CAAGGATGCC CATCTAATCC CGGACCACAC CATACGGGCG 
TTAGGACACA TCGCTGTGGC TCTGAGAGAC ACTCCACGAG TGATGGAGCC CATCTTACAG 
ATCCTGCAGC AGAAGTTCTG CCAGCCGCCG TCTCAGCTCG ACGTGCTCAT CATAGATCAG 
CTGGGCTGTA TGGTCATCAC TGGAAACCAA TATATTTATC AGGAAGTGTG GAACCTGTTC 
CAGCAGATCA GCGTGAAGGC CAGCTCCATG GTTTACTCCA CCAAAGACTA CAAGGATCAT 
GGATACAGGC ACTGTTCTCT GGCCGTGATC AATGCTCTGG CCAACATCGC AGCCAACCTG 
CAGACGGAGC AGATGGTGGA CGAGCTGCTG GTCAATCTGC TGGAACTGTT TGTGCAGCTG 
GGGCTGGAGG GCAAACGGGC CAGTGAAAGA GCCAGCGAGA AGGGCCCGGC CCTCAAGGCG 
TCAAGCAGCG CTGGTAATCT CGGAGTTCTC ATTCCTGTCA TTGCTGTGCT AACCAAACGT 
TTGCCGGCCA TAAAAGAAGC CAAACCGAGG CTGCAGAAGC TGTTCAGAGA TTTCTGGCTG 
TATTCGGTTG TGATGGGCTT CGCTGTTGAG GGCTCAGGTC TGTGGCCGGA GGAGTGGTAT 
GAGGGAGTGT GTGAGATTGC AACAAAATCC CCACTGCTGA CCTTCCCAAG CGGAGAACCG 
CTGCGCTCAG AGCTGCAGTA TAACTCGGCC CTGAAGAACG ACACTGTCAC GCCGGCTGAG 
CTGAATGATT TGAGGGGAAC CATCATTAAC CTGCTGGATC CTCCGCCGGA GGTTGCTGCG 
CTCATTAATA AACTGGACTT TGCCATGTCC ACCTACCTGC TGTCCGTCTA CCGGCTGGAA 
TACATGAGAG TGTTACGATC TCAGGAGTCT GACCGATTCC AAGTGATGTT TCGCTATTTT 
GAAGACCGAG CGATTCAGAA GGATAAATCA GGAATGATGC AGTGTGTCAT CGCAGTAGGT 
GACAAGGTCT TCGATGTCTT TCTTCAGATG ATGGCTGACA AGGCAAAGAC CAAAGCCCAT 
GAGGAGGAGC TGGAAAGTCA TGCTCAGTTT CTATTGGTCA ACTTCAACCA CATTCATAAG 
AGAATCCGAA GAGTTGCTGA CAAATATCTG TCAGGACTCG CTGAGACATT TCCACACTTG 
CTGTGGAGCG GCCGTGTTCT GAGGACCATG CTGGATATAT TACAGACTCT GTCTCTTTCG 
CTCAGTGCTG ATATCCACAA AGACCAACCA TATTACGACA TACCAGACAC ACCATATCGC 
ATCACAGTGC CGGACACGTA TGAGGCCAGA GAGAGCATAG TGAAGGACTT TGCCGCCCGC 
TGTGGAGAAA TCCTGAAAGA GGCCATGAAA TGGGCGCCAT CGGTCACCAA ATCACACCTA 
CAGGAATACC TGAATAAGCA TCAGAACTGG GTGTCGGGTC TGTCTCAGCA CACAGGGCTG 
GCGATGGCCA CAGAGAGCAT CCTGCACTTC GCCGGATACA ACCGTCAGAG CACCAGTCTC 
GGTACCACTC AGCTGACCGA GAGACCCGCA TGCGTGAAGA AAGACTACTC CAACTTCATG 
GCCTCCCTCA ACCTGAGAAA CCGCTACGCT GGAGAGGTCG CAGGGATGAT CCATTTCTCC 
GAGGCCACGC GGAGCACATC AGACCTGAAT AAACTGATGA TCCGACAGAT GAATGAAGCT 
CTGGATGGAG GACAGCCCGA AGCCTTCACC GAAGCCCTGT TCAAGATGGC AGCGCTGTTG 
ATCAGCTCCA GAGAATGGGA CCCTCTGCTG TTGCATCACC TGTGCTGGTC TCCATTAAAG 
ATGTTTACTG AGAATGGCAT GGAAACGGCT ATAGCCTGCT GGGAATGGCT TCTGGCTGGA 
AGAACAGGAG TTGAAGTCCC GTTCATGCGT GAGATGGCCG GCGCGTGGCA GATGACGGTG 
GATCTGAAGA TGGGGCTGTT TTCTGATGCC CAGCTGGAGG CCGATCCCCT GGCGGCTTCC 
GAGGAAAGCC AACCCATCCC CTGCCCGCCC GACGTCACGC CACACTACAT CTGGATTGAG 
TTTCTGGTGC AGCGGTTTGA GATCGCCAAG TACAGCAGCG CCGATCAGGT GGAGATTTTC 
GGCAGTCTTC TGCAGAGGTC CCTCTCTCTG AATGTCGGCA GCCCAAAAAG CAGTCTGAAC 
CGCCATGTGG CAGCCATCGG ACCTCGCTTC AGGTTGTTGA CGCTCGGACT TTCTCTCCTG 
CATTCTGACG TAGTGACCAA TGCCACCATC CGTAACGTCC TAAGGGAAAA GATTTATTCC 
ACTGCCTTCG ATTACTTCAG TGTGGCATCA AAGTTCCCCA CTCAGGGAGA CAAGCGTCTG 
CGGGAGGACA TCAGCATCAT GATCCGCTTC TACGCCAGCA TCCTCTCCGA CAAGAAGTAC 
CTAGGTGCTA GCCTGCTCGT GCCACCTGAC AACCAGGACC CGTCGCTGAA CAGTCTATCA 
GTGATGAACG CCACCGACCC CAGGAACAAC ATGGACATGA CGGTGGGCTC CCGCCAGCAG 
AGCGCTCAGG GCTGGATCAA CACGTACCCC CTGTCTAGCG GCATGTCCAC CACCTCTAAG 
AAATCAGGCA TGTCCAAAAA AAGCAACAGA GGAACCCAGC TGCACAAGTA CTACATGAAA 
CGCAGGACGT TGCTCTTGGC CTTGCTGGCC AGCGAGATCG AGAGACTGAG CACATGGTAC 
AACCCGCTGT CCACACAGGA GCTGGCCATT CCCACCGAGC AGTCTGTGGA AACCAGCATC 
TCCAACTGGA GGTCCAAATA CATCAACCTG ACCGAGAAAC AGTGGAAGGA TAATGTCAAT 
TTGGCCTGGA GTATCGCTCC TTACCTGGCC TTGCAGCTTC CGGCAAGGTT CAAAAACGCA 
GACGCCATTG TGGCAGAAGT GACTCGTCTA GTTCGGCTGG ATCCTGGTGC TGTCAGCGAT 
GTGCCAGAGG CAGTGAAGTT CCTGGTAACG TGGCACACCA TTGATGCAGA TTCTCCAGAG 
CTGAGCCACA TCTTGTGCTG GGCTCCTGCA GATCCTCCGA CCGGCCTCTC GTATTTCTCC 
AGCATGTATC CTCCTCATCC GCTCACTGCT CAGTATGGAG TCAAAGTCCT TCGCTCTTTT 
CCTCCTGATG CCATTTTGTT TTACATTCCT CAAATCGTTC AGGCGCTGCG CTACGACAAG 
ATGGGTTACG TGCGCGAGTA CATCCTGTGG GCCGCACAGA AGTCGCAGCT CCTCGCACAT 
CAGTTCATAT GGAACATGAA GACCAACATC TTTCTGGATG AAGAGGGACA TCAGAAAGAC 
CCGGACATCG GTGAGCTTCT GGAGCAGATG GTGGAAGAGA TCACGCACTC TCTGTCAGGT 
CCAGCTAAAG ACTTCTATCA GCGCGAGTTT GACTTCTTCA ACAAGATCAC CAACGTCTCC 
GCCATCATCA AGCCTGTTCC CAAAGGAGAT GAGAGGAAGA GAGCGTGTCT GAAGGCGCTG 
TCAGAAATCA AAGTTCAGCC AGGATGTTAT TTGCCAAGCA ATCCAGAAGC AATCGTCCTT 
GATATCGACT ACAAGTCAGG AACGCCCATG CAGAGCGCTG CCAAAGCCCC TTATCTGGCC 
AAATTCAAAG TTAAGAGGTG TGGCGTTAGT GAGCTTGAAA AAGAGGGTCT GCGCTGTCCG 
TCCGACTCTC AGGATGAAGG AGATGAGAGC TCACTGACGG CTCAAAGGGT CTGCTGGCAG 
GCGGCCATCT TTAAAGTGGG CGATGATTGC AGACAGGACA TGCTCGCGCT TCAGATCATT 
GGCCTGTTCA AGAACATCTT TCAGCTGGTG GGTTTGGATC TGTTTGTGTT CCCGTACCGG 
GTTGTGGCCA CAGCTCCAGG ATGTGGCGTG ATTGAATGTA TTCCGGACTG TAAATCCCGG 
GATCAGCTCG GCCGGCAGAC TGACTTCGGC ATGTACGACT ACTTCAGGAA CCAATATGGC 
GACGAATCCA CTCTGGCTTT CCAGAAGGCT CGGTATAACT TCATTCGCAG TATGGCTGCT 
TACAGTCTGC TGCTCTTTCT GTTACAAATC AAAGACCGAC ACAACGGCAA CATCATGCTG 
GACAGCAAAG GCCATCTCAT TCACATCGAC TTTGGCTTCA TGTTTGAGAG TTCTCCTGGT 
GGGAATCTGG GCTGGGAGCC TGACATCAAG CTGACCGATG AGATGGTGAT GATCATGGGA 
GGCAAGATGG AGGCTACACC ATTTAAATGG TTCATGGAGA TGTGTGTGCG CGGATACCTG 
GCTGTAAGGC CCTACATGGA TGCTGTCGTG TCATTGGTGA CGTTGATGCT GGACACGGGG 
TTGCCTTGCT TCAGAGGACA GACGATCAAA CTCCTCAAGC AACGGTTCAA CCCAAACATG 
AGCGAGAAGG AAGCTGCGGC CTTCATGATC AAAGTCATCG AGCGCTGCTT CCTCAGTAGC 
AGGAGCAAAA CCTACGACAT GCTGCAATAC TACCAGAACC AAATCCCATA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pi4kaa ( XM_005172596.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172653.1 Danio rerio phosphatidylinositol 4-kinase, catalytic, alpha a (pi4kaa), transcript variant X2, mRNA.

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CloneID	ODa15706
Clone ID Related Accession (Same CDS sequence)	XM_005172596.3 , XM_005172596.4
Accession Version	XM_005172596.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	phosphatidylinositol 4-kinase alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005172596.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCGA GAGGGACGTC GCGGGGTTTT TACTTTAACA CTGTGCTTTC TCTCGCGCGC 
TCACTGGCTG CTCATCGACC CGCTCCGATC GACAAGGTCC AGAAGCTACA ATGCATGTGT 
CCTGTGGACA TCCGTGGTGT TTTCCAGCTG GACGAGCGGA GGAGAGATGC TGTGATCGCA 
CTTGGGATCT TCCTAGTGGA GTCTGAGTTG CAGCACAAGG ATGCTATTGT CCCGTACTTG 
TTGGTTTTGC TAAAGGGATT GCCCAGGGTG CAATGGATCG AGGAGAGCTC AGAAAGGAAA 
GGACGAGAAG CGCTTCCCAT TGCGGAGAAT TTCTCCTTCT GTTTGGTGAC GGTGCTCTCA 
GACGTGGCCC AGATGGATGA ATCCATGCGC ATCCAGATTT TGGAGGCTGT GATGAACCTC 
ATTCAGGTGC TACAGGAAAC ATGTCAGAAC ATCGAGGGCC AAGATAAAGA ATACCTGTGT 
AAATACACCG TGCCCTGTCT GCTCGGTGTG GTGCGAGCGT TTGGCCGCTA CAGCAACACA 
GAGGAGCCGC TGTTGTCTAA ACTCTTCCCC CGGATCGTTC CTCAGCCTCC AGGCACCGGC 
GACGGCGCAG AGGTGACCCG ACGGAGATCT TTTAATGATT TCCGCTCCAT CCTGCCCAGC 
TCACTCCTCA CCGTTTGCCA AAGTGACACT TTACGCCGCA AAACCAGCAG CACTTCCAGC 
ATCTCACAAC AGGCCAGTCC AGAACGTGCT GGACTTCCTC CGAGTTCTCC CTCTGACCCT 
TCTGCTCCTT ATTTCGAAGC TGCCTCGTAT CTGCCCGACG GCAGTGCTGT GGACCCGGAC 
TACTATTTCT CCACCATCAG CTCCAGCTTC TCAGTCTCTC CGCTCTTCAC AGGCAGCAAC 
AATAAAGAGT TTACCGTACC GCTGGATTTA CTGAGGCAGC TTCTGCAGAT GGTCGAGCAG 
TTTGTGTCAG AATCCTTCAT GAAGACACTG GATCAGGCCA TGACTGAAGT TCTGGAGATC 
AATCCCACGG CTTATCTGTA CTACAAGACT TTCAGTGACC CGCTGTACGT GGCCATCTTT 
AAAATGCTGA GGGACTCGTT GTATTATATG AAAGATCTGC AGGTGTCGTT TGTGAAGGAG 
GTCCATGACT TTGTTCTGGA GCAGTTCAGC AGCAGTCAGT CTGAGCTGCA GAGGATCCTG 
CACGATGTGG AGTATCTGCA CAGTGAACTG AACCCGCTTA AGCTGCGCTG TCAGGCCAAC 
GCCGCCTGTG TGGACCTCAT GGTGTGGGCC GTCACGGAAG AACAGGGCGC TGAAAATCTC 
TGCACTAAAC TGTCAGAGAA GCTCCAGTCC AAGACGTCCA GCAAAGTCAT CATCGCCCAC 
ATGCCTCTGC TCATCTGCTG CTTACAGGGT CTCGGCCGGC TCTGTGAGCG TTTTCCTGTG 
GTGGCTCACT CTGTGACCAT GTCTCTACGA GATTTTCTGG TGGTTCCTTC ACCTGTACTA 
GTGAAGCTCT ACAAGTACCA CAGTCAGTAC ACCACAGGAG CAGGTGAGAT CAAGATTCAT 
GTCACCAACG AGCATTCCCA GTCCACCTTC AGCTCGCACT CCAGCAAGAA GAGCCAGCCG 
TCCATGTATG AGCAGCTCCG AGACATTTCC ATCAACAATA TATGCAGGTG TTTAAAAGCA 
GGATTGACCA TGGATTCAGT CATCGTTGAA GCTTTCCTCG CCAGTCTTTC CAACCGTCTG 
TACATTTCGC AGGAAAATGA CAAGGATGCC CATCTAATCC CGGACCACAC CATACGGGCG 
TTAGGACACA TCGCTGTGGC TCTGAGAGAC ACTCCACGAG TGATGGAGCC CATCTTACAG 
ATCCTGCAGC AGAAGTTCTG CCAGCCGCCG TCTCAGCTCG ACGTGCTCAT CATAGATCAG 
CTGGGCTGTA TGGTCATCAC TGGAAACCAA TATATTTATC AGGAAGTGTG GAACCTGTTC 
CAGCAGATCA GCGTGAAGGC CAGCTCCATG GTTTACTCCA CCAAAGACTA CAAGGATCAT 
GGATACAGGC ACTGTTCTCT GGCCGTGATC AATGCTCTGG CCAACATCGC AGCCAACCTG 
CAGACGGAGC AGATGGTGGA CGAGCTGCTG GTCAATCTGC TGGAACTGTT TGTGCAGCTG 
GGGCTGGAGG GCAAACGGGC CAGTGAAAGA GCCAGCGAGA AGGGCCCGGC CCTCAAGGCG 
TCAAGCAGCG CTGGTAATCT CGGAGTTCTC ATTCCTGTCA TTGCTGTGCT AACCAAACGT 
TTGCCGGCCA TAAAAGAAGC CAAACCGAGG CTGCAGAAGC TGTTCAGAGA TTTCTGGCTG 
TATTCGGTTG TGATGGGCTT CGCTGTTGAG GGCTCAGGTC TGTGGCCGGA GGAGTGGTAT 
GAGGGAGTGT GTGAGATTGC AACAAAATCC CCACTGCTGA CCTTCCCAAG CGGAGAACCG 
CTGCGCTCAG AGCTGCAGTA TAACTCGGCC CTGAAGAACG ACACTGTCAC GCCGGCTGAG 
CTGAATGATT TGAGGGGAAC CATCATTAAC CTGCTGGATC CTCCGCCGGA GGTTGCTGCG 
CTCATTAATA AACTGGACTT TGCCATGTCC ACCTACCTGC TGTCCGTCTA CCGGCTGGAA 
TACATGAGAG TGTTACGATC TCAGGAGTCT GACCGATTCC AAGTGATGTT TCGCTATTTT 
GAAGACCGAG CGATTCAGAA GGATAAATCA GGAATGATGC AGTGTGTCAT CGCAGTAGGT 
GACAAGGTCT TCGATGTCTT TCTTCAGATG ATGGCTGACA AGGCAAAGAC CAAAGCCCAT 
GAGGAGGAGC TGGAAAGTCA TGCTCAGTTT CTATTGGTCA ACTTCAACCA CATTCATAAG 
AGAATCCGAA GAGTTGCTGA CAAATATCTG TCAGGACTCG CTGAGACATT TCCACACTTG 
CTGTGGAGCG GCCGTGTTCT GAGGACCATG CTGGATATAT TACAGACTCT GTCTCTTTCG 
CTCAGTGCTG ATATCCACAA AGACCAACCA TATTACGACA TACCAGACAC ACCATATCGC 
ATCACAGTGC CGGACACGTA TGAGGCCAGA GAGAGCATAG TGAAGGACTT TGCCGCCCGC 
TGTGGAGAAA TCCTGAAAGA GGCCATGAAA TGGGCGCCAT CGGTCACCAA ATCACACCTA 
CAGGAATACC TGAATAAGCA TCAGAACTGG GTGTCGGGTC TGTCTCAGCA CACAGGGCTG 
GCGATGGCCA CAGAGAGCAT CCTGCACTTC GCCGGATACA ACCGTCAGAG CACCAGTCTC 
GGTACCACTC AGCTGACCGA GAGACCCGCA TGCGTGAAGA AAGACTACTC CAACTTCATG 
GCCTCCCTCA ACCTGAGAAA CCGCTACGCT GGAGAGGTCG CAGGGATGAT CCATTTCTCC 
GAGGCCACGC GGAGCACATC AGACCTGAAT AAACTGATGA TCCGACAGAT GAATGAAGCT 
CTGGATGGAG GACAGCCCGA AGCCTTCACC GAAGCCCTGT TCAAGATGGC AGCGCTGTTG 
ATCAGCTCCA GAGAATGGGA CCCTCTGCTG TTGCATCACC TGTGCTGGTC TCCATTAAAG 
ATGTTTACTG AGAATGGCAT GGAAACGGCT ATAGCCTGCT GGGAATGGCT TCTGGCTGGA 
AGAACAGGAG TTGAAGTCCC GTTCATGCGT GAGATGGCCG GCGCGTGGCA GATGACGGTG 
GATCTGAAGA TGGGGCTGTT TTCTGATGCC CAGCTGGAGG CCGATCCCCT GGCGGCTTCC 
GAGGAAAGCC AACCCATCCC CTGCCCGCCC GACGTCACGC CACACTACAT CTGGATTGAG 
TTTCTGGTGC AGCGGTTTGA GATCGCCAAG TACAGCAGCG CCGATCAGGT GGAGATTTTC 
GGCAGTCTTC TGCAGAGGTC CCTCTCTCTG AATGTCGGCA GCCCAAAAAG CAGTCTGAAC 
CGCCATGTGG CAGCCATCGG ACCTCGCTTC AGGTTGTTGA CGCTCGGACT TTCTCTCCTG 
CATTCTGACG TAGTGACCAA TGCCACCATC CGTAACGTCC TAAGGGAAAA GATTTATTCC 
ACTGCCTTCG ATTACTTCAG TGTGGCATCA AAGTTCCCCA CTCAGGGAGA CAAGCGTCTG 
CGGGAGGACA TCAGCATCAT GATCCGCTTC TACGCCAGCA TCCTCTCCGA CAAGAAGTAC 
CTAGGTGCTA GCCTGCTCGT GCCACCTGAC AACCAGGACC CGTCGCTGAA CAGTCTATCA 
GTGATGAACG CCACCGACCC CAGGAACAAC ATGGACATGA CGGTGGGCTC CCGCCAGCAG 
AGCGCTCAGG GCTGGATCAA CACGTACCCC CTGTCTAGCG GCATGTCCAC CACCTCTAAG 
AAATCAGGCA TGTCCAAAAA AAGCAACAGA GGAACCCAGC TGCACAAGTA CTACATGAAA 
CGCAGGACGT TGCTCTTGGC CTTGCTGGCC AGCGAGATCG AGAGACTGAG CACATGGTAC 
AACCCGCTGT CCACACAGGA GCTGGCCATT CCCACCGAGC AGTCTGTGGA AACCAGCATC 
TCCAACTGGA GGTCCAAATA CATCAACCTG ACCGAGAAAC AGTGGAAGGA TAATGTCAAT 
TTGGCCTGGA GTATCGCTCC TTACCTGGCC TTGCAGCTTC CGGCAAGGTT CAAAAACGCA 
GACGCCATTG TGGCAGAAGT GACTCGTCTA GTTCGGCTGG ATCCTGGTGC TGTCAGCGAT 
GTGCCAGAGG CAGTGAAGTT CCTGGTAACG TGGCACACCA TTGATGCAGA TTCTCCAGAG 
CTGAGCCACA TCTTGTGCTG GGCTCCTGCA GATCCTCCGA CCGGCCTCTC GTATTTCTCC 
AGCATGTATC CTCCTCATCC GCTCACTGCT CAGTATGGAG TCAAAGTCCT TCGCTCTTTT 
CCTCCTGATG CCATTTTGTT TTACATTCCT CAAATCGTTC AGGCGCTGCG CTACGACAAG 
ATGGGTTACG TGCGCGAGTA CATCCTGTGG GCCGCACAGA AGTCGCAGCT CCTCGCACAT 
CAGTTCATAT GGAACATGAA GACCAACATC TTTCTGGATG AAGAGGGACA TCAGAAAGAC 
CCGGACATCG GTGAGCTTCT GGAGCAGATG GTGGAAGAGA TCACGCACTC TCTGTCAGGT 
CCAGCTAAAG ACTTCTATCA GCGCGAGTTT GACTTCTTCA ACAAGATCAC CAACGTCTCC 
GCCATCATCA AGCCTGTTCC CAAAGGAGAT GAGAGGAAGA GAGCGTGTCT GAAGGCGCTG 
TCAGAAATCA AAGTTCAGCC AGGATGTTAT TTGCCAAGCA ATCCAGAAGC AATCGTCCTT 
GATATCGACT ACAAGTCAGG AACGCCCATG CAGAGCGCTG CCAAAGCCCC TTATCTGGCC 
AAATTCAAAG TTAAGAGGTG TGGCGTTAGT GAGCTTGAAA AAGAGGGTCT GCGCTGTCCG 
TCCGACTCTC AGGATGAAGG AGATGAGAGC TCACTGACGG CTCAAAGGGT CTGCTGGCAG 
GCGGCCATCT TTAAAGTGGG CGATGATTGC AGACAGGACA TGCTCGCGCT TCAGATCATT 
GGCCTGTTCA AGAACATCTT TCAGCTGGTG GGTTTGGATC TGTTTGTGTT CCCGTACCGG 
GTTGTGGCCA CAGCTCCAGG ATGTGGCGTG ATTGAATGTA TTCCGGACTG TAAATCCCGG 
GATCAGCTCG GCCGGCAGAC TGACTTCGGC ATGTACGACT ACTTCAGGAA CCAATATGGC 
GACGAATCCA CTCTGGCTTT CCAGAAGGCT CGGTATAACT TCATTCGCAG TATGGCTGCT 
TACAGTCTGC TGCTCTTTCT GTTACAAATC AAAGACCGAC ACAACGGCAA CATCATGCTG 
GACAGCAAAG GCCATCTCAT TCACATCGAC TTTGGCTTCA TGTTTGAGAG TTCTCCTGGT 
GGGAATCTGG GCTGGGAGCC TGACATCAAG CTGACCGATG AGATGGTGAT GATCATGGGA 
GGCAAGATGG AGGCTACACC ATTTAAATGG TTCATGGAGA TGTGTGTGCG CGGATACCTG 
GCTGTAAGGC CCTACATGGA TGCTGTCGTG TCATTGGTGA CGTTGATGCT GGACACGGGG 
TTGCCTTGCT TCAGAGGACA GACGATCAAA CTCCTCAAGC AACGGTTCAA CCCAAACATG 
AGCGAGAAGG AAGCTGCGGC CTTCATGATC AAAGTCATCG AGCGCTGCTT CCTCAGTAGC 
AGGAGCAAAA CCTACGACAT GCTGCAATAC TACCAGAACC AAATCCCATA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172653.1
CDS	115..6408
Translation	MSSRGTSRGFYFNTVLSLARSLAAHRPAPIDKVQKLQCMCPVDIRGVFQLDERRRDAVIALGIFLVESELQHKDAIVPYLLVLLKGLPRVQWIEESSERKGREALPIAENFSFCLVTVLSDVAQMDESMRIQILEAVMNLIQVLQETCQNIEGQDKEYLCKYTVPCLLGVVRAFGRYSNTEEPLLSKLFPRIVPQPPGTGDGAEVTRRRSFNDFRSILPSSLLTVCQSDTLRRKTSSTSSISQQASPERAGLPPSSPSDPSAPYFEAASYLPDGSAVDPDYYFSTISSSFSVSPLFTGSNNKEFTVPLDLLRQLLQMVEQFVSESFMKTLDQAMTEVLEINPTAYLYYKTFSDPLYVAIFKMLRDSLYYMKDLQVSFVKEVHDFVLEQFSSSQSELQRILHDVEYLHSELNPLKLRCQANAACVDLMVWAVTEEQGAENLCTKLSEKLQSKTSSKVIIAHMPLLICCLQGLGRLCERFPVVAHSVTMSLRDFLVVPSPVLVKLYKYHSQYTTGAGEIKIHVTNEHSQSTFSSHSSKKSQPSMYEQLRDISINNICRCLKAGLTMDSVIVEAFLASLSNRLYISQENDKDAHLIPDHTIRALGHIAVALRDTPRVMEPILQILQQKFCQPPSQLDVLIIDQLGCMVITGNQYIYQEVWNLFQQISVKASSMVYSTKDYKDHGYRHCSLAVINALANIAANLQTEQMVDELLVNLLELFVQLGLEGKRASERASEKGPALKASSSAGNLGVLIPVIAVLTKRLPAIKEAKPRLQKLFRDFWLYSVVMGFAVEGSGLWPEEWYEGVCEIATKSPLLTFPSGEPLRSELQYNSALKNDTVTPAELNDLRGTIINLLDPPPEVAALINKLDFAMSTYLLSVYRLEYMRVLRSQESDRFQVMFRYFEDRAIQKDKSGMMQCVIAVGDKVFDVFLQMMADKAKTKAHEEELESHAQFLLVNFNHIHKRIRRVADKYLSGLAETFPHLLWSGRVLRTMLDILQTLSLSLSADIHKDQPYYDIPDTPYRITVPDTYEARESIVKDFAARCGEILKEAMKWAPSVTKSHLQEYLNKHQNWVSGLSQHTGLAMATESILHFAGYNRQSTSLGTTQLTERPACVKKDYSNFMASLNLRNRYAGEVAGMIHFSEATRSTSDLNKLMIRQMNEALDGGQPEAFTEALFKMAALLISSREWDPLLLHHLCWSPLKMFTENGMETAIACWEWLLAGRTGVEVPFMREMAGAWQMTVDLKMGLFSDAQLEADPLAASEESQPIPCPPDVTPHYIWIEFLVQRFEIAKYSSADQVEIFGSLLQRSLSLNVGSPKSSLNRHVAAIGPRFRLLTLGLSLLHSDVVTNATIRNVLREKIYSTAFDYFSVASKFPTQGDKRLREDISIMIRFYASILSDKKYLGASLLVPPDNQDPSLNSLSVMNATDPRNNMDMTVGSRQQSAQGWINTYPLSSGMSTTSKKSGMSKKSNRGTQLHKYYMKRRTLLLALLASEIERLSTWYNPLSTQELAIPTEQSVETSISNWRSKYINLTEKQWKDNVNLAWSIAPYLALQLPARFKNADAIVAEVTRLVRLDPGAVSDVPEAVKFLVTWHTIDADSPELSHILCWAPADPPTGLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGYVREYILWAAQKSQLLAHQFIWNMKTNIFLDEEGHQKDPDIGELLEQMVEEITHSLSGPAKDFYQREFDFFNKITNVSAIIKPVPKGDERKRACLKALSEIKVQPGCYLPSNPEAIVLDIDYKSGTPMQSAAKAPYLAKFKVKRCGVSELEKEGLRCPSDSQDEGDESSLTAQRVCWQAAIFKVGDDCRQDMLALQIIGLFKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPDCKSRDQLGRQTDFGMYDYFRNQYGDESTLAFQKARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDSKGHLIHIDFGFMFESSPGGNLGWEPDIKLTDEMVMIMGGKMEATPFKWFMEMCVRGYLAVRPYMDAVVSLVTLMLDTGLPCFRGQTIKLLKQRFNPNMSEKEAAAFMIKVIERCFLSSRSKTYDMLQYYQNQIPY

Target ORF information:

RefSeq Version	XM_005172596.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphatidylinositol 4-kinase, catalytic, alpha a (pi4kaa), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172596.4

ORF Insert Sequence:

ATGTCGTCGA GAGGGACGTC GCGGGGTTTT TACTTTAACA CTGTGCTTTC TCTCGCGCGC 
TCACTGGCTG CTCATCGACC CGCTCCGATC GACAAGGTCC AGAAGCTACA ATGCATGTGT 
CCTGTGGACA TCCGTGGTGT TTTCCAGCTG GACGAGCGGA GGAGAGATGC TGTGATCGCA 
CTTGGGATCT TCCTAGTGGA GTCTGAGTTG CAGCACAAGG ATGCTATTGT CCCGTACTTG 
TTGGTTTTGC TAAAGGGATT GCCCAGGGTG CAATGGATCG AGGAGAGCTC AGAAAGGAAA 
GGACGAGAAG CGCTTCCCAT TGCGGAGAAT TTCTCCTTCT GTTTGGTGAC GGTGCTCTCA 
GACGTGGCCC AGATGGATGA ATCCATGCGC ATCCAGATTT TGGAGGCTGT GATGAACCTC 
ATTCAGGTGC TACAGGAAAC ATGTCAGAAC ATCGAGGGCC AAGATAAAGA ATACCTGTGT 
AAATACACCG TGCCCTGTCT GCTCGGTGTG GTGCGAGCGT TTGGCCGCTA CAGCAACACA 
GAGGAGCCGC TGTTGTCTAA ACTCTTCCCC CGGATCGTTC CTCAGCCTCC AGGCACCGGC 
GACGGCGCAG AGGTGACCCG ACGGAGATCT TTTAATGATT TCCGCTCCAT CCTGCCCAGC 
TCACTCCTCA CCGTTTGCCA AAGTGACACT TTACGCCGCA AAACCAGCAG CACTTCCAGC 
ATCTCACAAC AGGCCAGTCC AGAACGTGCT GGACTTCCTC CGAGTTCTCC CTCTGACCCT 
TCTGCTCCTT ATTTCGAAGC TGCCTCGTAT CTGCCCGACG GCAGTGCTGT GGACCCGGAC 
TACTATTTCT CCACCATCAG CTCCAGCTTC TCAGTCTCTC CGCTCTTCAC AGGCAGCAAC 
AATAAAGAGT TTACCGTACC GCTGGATTTA CTGAGGCAGC TTCTGCAGAT GGTCGAGCAG 
TTTGTGTCAG AATCCTTCAT GAAGACACTG GATCAGGCCA TGACTGAAGT TCTGGAGATC 
AATCCCACGG CTTATCTGTA CTACAAGACT TTCAGTGACC CGCTGTACGT GGCCATCTTT 
AAAATGCTGA GGGACTCGTT GTATTATATG AAAGATCTGC AGGTGTCGTT TGTGAAGGAG 
GTCCATGACT TTGTTCTGGA GCAGTTCAGC AGCAGTCAGT CTGAGCTGCA GAGGATCCTG 
CACGATGTGG AGTATCTGCA CAGTGAACTG AACCCGCTTA AGCTGCGCTG TCAGGCCAAC 
GCCGCCTGTG TGGACCTCAT GGTGTGGGCC GTCACGGAAG AACAGGGCGC TGAAAATCTC 
TGCACTAAAC TGTCAGAGAA GCTCCAGTCC AAGACGTCCA GCAAAGTCAT CATCGCCCAC 
ATGCCTCTGC TCATCTGCTG CTTACAGGGT CTCGGCCGGC TCTGTGAGCG TTTTCCTGTG 
GTGGCTCACT CTGTGACCAT GTCTCTACGA GATTTTCTGG TGGTTCCTTC ACCTGTACTA 
GTGAAGCTCT ACAAGTACCA CAGTCAGTAC ACCACAGGAG CAGGTGAGAT CAAGATTCAT 
GTCACCAACG AGCATTCCCA GTCCACCTTC AGCTCGCACT CCAGCAAGAA GAGCCAGCCG 
TCCATGTATG AGCAGCTCCG AGACATTTCC ATCAACAATA TATGCAGGTG TTTAAAAGCA 
GGATTGACCA TGGATTCAGT CATCGTTGAA GCTTTCCTCG CCAGTCTTTC CAACCGTCTG 
TACATTTCGC AGGAAAATGA CAAGGATGCC CATCTAATCC CGGACCACAC CATACGGGCG 
TTAGGACACA TCGCTGTGGC TCTGAGAGAC ACTCCACGAG TGATGGAGCC CATCTTACAG 
ATCCTGCAGC AGAAGTTCTG CCAGCCGCCG TCTCAGCTCG ACGTGCTCAT CATAGATCAG 
CTGGGCTGTA TGGTCATCAC TGGAAACCAA TATATTTATC AGGAAGTGTG GAACCTGTTC 
CAGCAGATCA GCGTGAAGGC CAGCTCCATG GTTTACTCCA CCAAAGACTA CAAGGATCAT 
GGATACAGGC ACTGTTCTCT GGCCGTGATC AATGCTCTGG CCAACATCGC AGCCAACCTG 
CAGACGGAGC AGATGGTGGA CGAGCTGCTG GTCAATCTGC TGGAACTGTT TGTGCAGCTG 
GGGCTGGAGG GCAAACGGGC CAGTGAAAGA GCCAGCGAGA AGGGCCCGGC CCTCAAGGCG 
TCAAGCAGCG CTGGTAATCT CGGAGTTCTC ATTCCTGTCA TTGCTGTGCT AACCAAACGT 
TTGCCGGCCA TAAAAGAAGC CAAACCGAGG CTGCAGAAGC TGTTCAGAGA TTTCTGGCTG 
TATTCGGTTG TGATGGGCTT CGCTGTTGAG GGCTCAGGTC TGTGGCCGGA GGAGTGGTAT 
GAGGGAGTGT GTGAGATTGC AACAAAATCC CCACTGCTGA CCTTCCCAAG CGGAGAACCG 
CTGCGCTCAG AGCTGCAGTA TAACTCGGCC CTGAAGAACG ACACTGTCAC GCCGGCTGAG 
CTGAATGATT TGAGGGGAAC CATCATTAAC CTGCTGGATC CTCCGCCGGA GGTTGCTGCG 
CTCATTAATA AACTGGACTT TGCCATGTCC ACCTACCTGC TGTCCGTCTA CCGGCTGGAA 
TACATGAGAG TGTTACGATC TCAGGAGTCT GACCGATTCC AAGTGATGTT TCGCTATTTT 
GAAGACCGAG CGATTCAGAA GGATAAATCA GGAATGATGC AGTGTGTCAT CGCAGTAGGT 
GACAAGGTCT TCGATGTCTT TCTTCAGATG ATGGCTGACA AGGCAAAGAC CAAAGCCCAT 
GAGGAGGAGC TGGAAAGTCA TGCTCAGTTT CTATTGGTCA ACTTCAACCA CATTCATAAG 
AGAATCCGAA GAGTTGCTGA CAAATATCTG TCAGGACTCG CTGAGACATT TCCACACTTG 
CTGTGGAGCG GCCGTGTTCT GAGGACCATG CTGGATATAT TACAGACTCT GTCTCTTTCG 
CTCAGTGCTG ATATCCACAA AGACCAACCA TATTACGACA TACCAGACAC ACCATATCGC 
ATCACAGTGC CGGACACGTA TGAGGCCAGA GAGAGCATAG TGAAGGACTT TGCCGCCCGC 
TGTGGAGAAA TCCTGAAAGA GGCCATGAAA TGGGCGCCAT CGGTCACCAA ATCACACCTA 
CAGGAATACC TGAATAAGCA TCAGAACTGG GTGTCGGGTC TGTCTCAGCA CACAGGGCTG 
GCGATGGCCA CAGAGAGCAT CCTGCACTTC GCCGGATACA ACCGTCAGAG CACCAGTCTC 
GGTACCACTC AGCTGACCGA GAGACCCGCA TGCGTGAAGA AAGACTACTC CAACTTCATG 
GCCTCCCTCA ACCTGAGAAA CCGCTACGCT GGAGAGGTCG CAGGGATGAT CCATTTCTCC 
GAGGCCACGC GGAGCACATC AGACCTGAAT AAACTGATGA TCCGACAGAT GAATGAAGCT 
CTGGATGGAG GACAGCCCGA AGCCTTCACC GAAGCCCTGT TCAAGATGGC AGCGCTGTTG 
ATCAGCTCCA GAGAATGGGA CCCTCTGCTG TTGCATCACC TGTGCTGGTC TCCATTAAAG 
ATGTTTACTG AGAATGGCAT GGAAACGGCT ATAGCCTGCT GGGAATGGCT TCTGGCTGGA 
AGAACAGGAG TTGAAGTCCC GTTCATGCGT GAGATGGCCG GCGCGTGGCA GATGACGGTG 
GATCTGAAGA TGGGGCTGTT TTCTGATGCC CAGCTGGAGG CCGATCCCCT GGCGGCTTCC 
GAGGAAAGCC AACCCATCCC CTGCCCGCCC GACGTCACGC CACACTACAT CTGGATTGAG 
TTTCTGGTGC AGCGGTTTGA GATCGCCAAG TACAGCAGCG CCGATCAGGT GGAGATTTTC 
GGCAGTCTTC TGCAGAGGTC CCTCTCTCTG AATGTCGGCA GCCCAAAAAG CAGTCTGAAC 
CGCCATGTGG CAGCCATCGG ACCTCGCTTC AGGTTGTTGA CGCTCGGACT TTCTCTCCTG 
CATTCTGACG TAGTGACCAA TGCCACCATC CGTAACGTCC TAAGGGAAAA GATTTATTCC 
ACTGCCTTCG ATTACTTCAG TGTGGCATCA AAGTTCCCCA CTCAGGGAGA CAAGCGTCTG 
CGGGAGGACA TCAGCATCAT GATCCGCTTC TACGCCAGCA TCCTCTCCGA CAAGAAGTAC 
CTAGGTGCTA GCCTGCTCGT GCCACCTGAC AACCAGGACC CGTCGCTGAA CAGTCTATCA 
GTGATGAACG CCACCGACCC CAGGAACAAC ATGGACATGA CGGTGGGCTC CCGCCAGCAG 
AGCGCTCAGG GCTGGATCAA CACGTACCCC CTGTCTAGCG GCATGTCCAC CACCTCTAAG 
AAATCAGGCA TGTCCAAAAA AAGCAACAGA GGAACCCAGC TGCACAAGTA CTACATGAAA 
CGCAGGACGT TGCTCTTGGC CTTGCTGGCC AGCGAGATCG AGAGACTGAG CACATGGTAC 
AACCCGCTGT CCACACAGGA GCTGGCCATT CCCACCGAGC AGTCTGTGGA AACCAGCATC 
TCCAACTGGA GGTCCAAATA CATCAACCTG ACCGAGAAAC AGTGGAAGGA TAATGTCAAT 
TTGGCCTGGA GTATCGCTCC TTACCTGGCC TTGCAGCTTC CGGCAAGGTT CAAAAACGCA 
GACGCCATTG TGGCAGAAGT GACTCGTCTA GTTCGGCTGG ATCCTGGTGC TGTCAGCGAT 
GTGCCAGAGG CAGTGAAGTT CCTGGTAACG TGGCACACCA TTGATGCAGA TTCTCCAGAG 
CTGAGCCACA TCTTGTGCTG GGCTCCTGCA GATCCTCCGA CCGGCCTCTC GTATTTCTCC 
AGCATGTATC CTCCTCATCC GCTCACTGCT CAGTATGGAG TCAAAGTCCT TCGCTCTTTT 
CCTCCTGATG CCATTTTGTT TTACATTCCT CAAATCGTTC AGGCGCTGCG CTACGACAAG 
ATGGGTTACG TGCGCGAGTA CATCCTGTGG GCCGCACAGA AGTCGCAGCT CCTCGCACAT 
CAGTTCATAT GGAACATGAA GACCAACATC TTTCTGGATG AAGAGGGACA TCAGAAAGAC 
CCGGACATCG GTGAGCTTCT GGAGCAGATG GTGGAAGAGA TCACGCACTC TCTGTCAGGT 
CCAGCTAAAG ACTTCTATCA GCGCGAGTTT GACTTCTTCA ACAAGATCAC CAACGTCTCC 
GCCATCATCA AGCCTGTTCC CAAAGGAGAT GAGAGGAAGA GAGCGTGTCT GAAGGCGCTG 
TCAGAAATCA AAGTTCAGCC AGGATGTTAT TTGCCAAGCA ATCCAGAAGC AATCGTCCTT 
GATATCGACT ACAAGTCAGG AACGCCCATG CAGAGCGCTG CCAAAGCCCC TTATCTGGCC 
AAATTCAAAG TTAAGAGGTG TGGCGTTAGT GAGCTTGAAA AAGAGGGTCT GCGCTGTCCG 
TCCGACTCTC AGGATGAAGG AGATGAGAGC TCACTGACGG CTCAAAGGGT CTGCTGGCAG 
GCGGCCATCT TTAAAGTGGG CGATGATTGC AGACAGGACA TGCTCGCGCT TCAGATCATT 
GGCCTGTTCA AGAACATCTT TCAGCTGGTG GGTTTGGATC TGTTTGTGTT CCCGTACCGG 
GTTGTGGCCA CAGCTCCAGG ATGTGGCGTG ATTGAATGTA TTCCGGACTG TAAATCCCGG 
GATCAGCTCG GCCGGCAGAC TGACTTCGGC ATGTACGACT ACTTCAGGAA CCAATATGGC 
GACGAATCCA CTCTGGCTTT CCAGAAGGCT CGGTATAACT TCATTCGCAG TATGGCTGCT 
TACAGTCTGC TGCTCTTTCT GTTACAAATC AAAGACCGAC ACAACGGCAA CATCATGCTG 
GACAGCAAAG GCCATCTCAT TCACATCGAC TTTGGCTTCA TGTTTGAGAG TTCTCCTGGT 
GGGAATCTGG GCTGGGAGCC TGACATCAAG CTGACCGATG AGATGGTGAT GATCATGGGA 
GGCAAGATGG AGGCTACACC ATTTAAATGG TTCATGGAGA TGTGTGTGCG CGGATACCTG 
GCTGTAAGGC CCTACATGGA TGCTGTCGTG TCATTGGTGA CGTTGATGCT GGACACGGGG 
TTGCCTTGCT TCAGAGGACA GACGATCAAA CTCCTCAAGC AACGGTTCAA CCCAAACATG 
AGCGAGAAGG AAGCTGCGGC CTTCATGATC AAAGTCATCG AGCGCTGCTT CCTCAGTAGC 
AGGAGCAAAA CCTACGACAT GCTGCAATAC TACCAGAACC AAATCCCATA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pi4kaa ( XM_009305081.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303356.1 Danio rerio phosphatidylinositol 4-kinase, catalytic, alpha a (pi4kaa), transcript variant X1, mRNA.

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CloneID	ODa42467
Clone ID Related Accession (Same CDS sequence)	XM_009305081.3 , XM_009305081.2
Accession Version	XM_009305081.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	phosphatidylinositol 4-kinase alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009305081.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCGA GAGGGACGTC GCGGGGTTTT TACTTTAACA CTGTGCTTTC TCTCGCGCGC 
TCACTGGCTG CTCATCGACC CGCTCCGATC GACAAGGTCC AGAAGCTACA ATGCATGTGT 
CCTGTGGACA TCCGTGGTGT TTTCCAGCTG GACGAGCGGA GGAGAGATGC TGTGATCGCA 
CTTGGGATCT TCCTAGTGGA GTCTGAGTTG CAGCACAAGG ATGCTATTGT CCCGTACTTG 
TTGGTTTTGC TAAAGGGATT GCCCAGGGTG CAATGGATCG AGGAGAGCTC AGAAAGGAAA 
GGACGAGAAG CGCTTCCCAT TGCGGAGAAT TTCTCCTTCT GTTTGGTGAC GGTGCTCTCA 
GACGTGGCCC AGATGGATGA ATCCATGCGC ATCCAGATTT TGGAGGCTGT GATGAACCTC 
ATTCAGGTGC TACAGGAAAC ATGTCAGAAC ATCGAGGGCC AAGATAAAGA ATACCTGTGT 
AAATACACCG TGCCCTGTCT GCTCGGTGTG GTGCGAGCGT TTGGCCGCTA CAGCAACACA 
GAGGAGCCGC TGTTGTCTAA ACTCTTCCCC CGGATCGTTC CTCAGCCTCC AGGCACCGGC 
GACGGCGCAG AGGTGACCCG ACGGAGATCT TTTAATGATT TCCGCTCCAT CCTGCCCAGC 
TCACTCCTCA CCGTTTGCCA AAGTGACACT TTACGCCGCA AAACCAGCAG CACTTCCAGC 
ATCTCACAAC AGGCCAGTCC AGAACGTGCT GGACTTCCTC CGAGTTCTCC CTCTGACCCT 
TCTGCTCCTT ATTTCGAAGC TGCCTCGTAT CTGCCCGACG GCAGTGCTGT GGACCCGGAC 
TACTATTTCT CCACCATCAG CTCCAGCTTC TCAGTCTCTC CGCTCTTCAC AGGCAGCAAC 
AATAAAGAGT TTACCGTACC GCTGGATTTA CTGAGGCAGC TTCTGCAGAT GGTCGAGCAG 
TTTGTGTCAG AATCCTTCAT GAAGACACTG GATCAGGCCA TGACTGAAGT TCTGGAGATC 
AATCCCACGG CTTATCTGTA CTACAAGACT TTCAGTGACC CGCTGTACGT GGCCATCTTT 
AAAATGCTGA GGGACTCGTT GTATTATATG AAAGATCTGC AGGTGTCGTT TGTGAAGGAG 
GTCCATGACT TTGTTCTGGA GCAGTTCAGC AGCAGTCAGT CTGAGCTGCA GAGGATCCTG 
CACGATGTGG AGTATCTGCA CAGTGAACTG AACCCGCTTA AGCTGCGCTG TCAGGCCAAC 
GCCGCCTGTG TGGACCTCAT GGTGTGGGCC GTCACGGAAG AACAGGGCGC TGAAAATCTC 
TGCACTAAAC TGTCAGAGAA GCTCCAGTCC AAGACGTCCA GCAAAGTCAT CATCGCCCAC 
ATGCCTCTGC TCATCTGCTG CTTACAGGGT CTCGGCCGGC TCTGTGAGCG TTTTCCTGTG 
GTGGCTCACT CTGTGACCAT GTCTCTACGA GATTTTCTGG TGGTTCCTTC ACCTGTACTA 
GTGAAGCTCT ACAAGTACCA CAGTCAGTAC ACCACAGGAG CAGGTGAGAT CAAGATTCAT 
GTCACCAACG AGCATTCCCA GTCCACCTTC AGCTCGCACT CCAGCAAGAA GAGCCAGCCG 
TCCATGTATG AGCAGCTCCG AGACATTTCC ATCAACAATA TATGCAGGTG TTTAAAAGCA 
GGATTGACCA TGGATTCAGT CATCGTTGAA GCTTTCCTCG CCAGTCTTTC CAACCGTCTG 
TACATTTCGC AGGAAAATGA CAAGGATGCC CATCTAATCC CGGACCACAC CATACGGGCG 
TTAGGACACA TCGCTGTGGC TCTGAGAGAC ACTCCACGAG TGATGGAGCC CATCTTACAG 
ATCCTGCAGC AGAAGTTCTG CCAGCCGCCG TCTCAGCTCG ACGTGCTCAT CATAGATCAG 
CTGGGCTGTA TGGTCATCAC TGGAAACCAA TATATTTATC AGGAAGTGTG GAACCTGTTC 
CAGCAGATCA GCGTGAAGGC CAGCTCCATG GTTTACTCCA CCAAAGACTA CAAGGATCAT 
GGATACAGGC ACTGTTCTCT GGCCGTGATC AATGCTCTGG CCAACATCGC AGCCAACCTG 
CAGACGGAGC AGATGGTGGA CGAGCTGCTG GTCAATCTGC TGGAACTGTT TGTGCAGCTG 
GGGCTGGAGG GCAAACGGGC CAGTGAAAGA GCCAGCGAGA AGGGCCCGGC CCTCAAGGCG 
TCAAGCAGCG CTGGTAATCT CGGAGTTCTC ATTCCTGTCA TTGCTGTGCT AACCAAACGT 
TTGCCGGCCA TAAAAGAAGC CAAACCGAGG CTGCAGAAGC TGTTCAGAGA TTTCTGGCTG 
TATTCGGTTG TGATGGGCTT CGCTGTTGAG GGCTCAGGTC TGTGGCCGGA GGAGTGGTAT 
GAGGGAGTGT GTGAGATTGC AACAAAATCC CCACTGCTGA CCTTCCCAAG CGGAGAACCG 
CTGCGCTCAG AGCTGCAGTA TAACTCGGCC CTGAAGAACG ACACTGTCAC GCCGGCTGAG 
CTGAATGATT TGAGGGGAAC CATCATTAAC CTGCTGGATC CTCCGCCGGA GGTTGCTGCG 
CTCATTAATA AACTGGACTT TGCCATGTCC ACCTACCTGC TGTCCGTCTA CCGGCTGGAA 
TACATGAGAG TGTTACGATC TCAGGAGTCT GACCGATTCC AAGTGATGTT TCGCTATTTT 
GAAGACCGAG CGATTCAGAA GGATAAATCA GGAATGATGC AGTGTGTCAT CGCAGTAGGT 
GACAAGGTCT TCGATGTCTT TCTTCAGATG ATGGCTGACA AGGCAAAGAC CAAAGCCCAT 
GAGGAGGAGC TGGAAAGTCA TGCTCAGTTT CTATTGGTCA ACTTCAACCA CATTCATAAG 
AGAATCCGAA GAGTTGCTGA CAAATATCTG TCAGGACTCG CTGAGACATT TCCACACTTG 
CTGTGGAGCG GCCGTGTTCT GAGGACCATG CTGGATATAT TACAGACTCT GTCTCTTTCG 
CTCAGTGCTG ATATCCACAA AGACCAACCA TATTACGACA TACCAGACAC ACCATATCGC 
ATCACAGTGC CGGACACGTA TGAGGCCAGA GAGAGCATAG TGAAGGACTT TGCCGCCCGC 
TGTGGAGAAA TCCTGAAAGA GGCCATGAAA TGGGCGCCAT CGGTCACCAA ATCACACCTA 
CAGGAATACC TGAATAAGCA TCAGAACTGG GTGTCGGGTC TGTCTCAGCA CACAGGGCTG 
GCGATGGCCA CAGAGAGCAT CCTGCACTTC GCCGGATACA ACCGTCAGAG CACCAGTCTC 
GGTACCACTC AGCTGACCGA GAGACCCGCA TGCGTGAAGA AAGACTACTC CAACTTCATG 
GCCTCCCTCA ACCTGAGAAA CCGCTACGCT GGAGAGGTCG CAGGGATGAT CCATTTCTCC 
GAGGCCACGC GGAGCACATC AGACCTGAAT AAACTGATGA TCCGACAGAT GAATGAAGCT 
CTGGATGGAG GACAGCCCGA AGCCTTCACC GAAGCCCTGT TCAAGATGGC AGCGCTGTTG 
ATCAGCTCCA GAGAATGGGA CCCTCTGCTG TTGCATCACC TGTGCTGGTC TCCATTAAAG 
ATGTTTACTG AGAATGGCAT GGAAACGGCT ATAGCCTGCT GGGAATGGCT TCTGGCTGGA 
AGAACAGGAG TTGAAGTCCC GTTCATGCGT GAGATGGCCG GCGCGTGGCA GATGACGGTG 
GATCTGAAGA TGGGGCTGTT TTCTGATGCC CAGCTGGAGG CCGATCCCCT GGCGGCTTCC 
GAGGAAAGCC AACCCATCCC CTGCCCGCCC GACGTCACGC CACACTACAT CTGGATTGAG 
TTTCTGGTGC AGCGGTTTGA GATCGCCAAG TACAGCAGCG CCGATCAGGT GGAGATTTTC 
GGCAGTCTTC TGCAGAGGTC CCTCTCTCTG AATGTCGGCA GCCCAAAAAG CAGTCTGAAC 
CGCCATGTGG CAGCCATCGG ACCTCGCTTC AGGTTGTTGA CGCTCGGACT TTCTCTCCTG 
CATTCTGACG TAGTGACCAA TGCCACCATC CGTAACGTCC TAAGGGAAAA GATTTATTCC 
ACTGCCTTCG ATTACTTCAG TGTGGCATCA AAGTTCCCCA CTCAGGGAGA CAAGCGTCTG 
CGGGAGGACA TCAGCATCAT GATCCGCTTC TACGCCAGCA TCCTCTCCGA CAAGAAGTAC 
CTAGGTGCTA GCCTGCTCGT GCCACCTGGT GAGCTCGGTG CCAATCCCCC TCAAAGCTCC 
CTATCTGCCG GGATTTTCGG ATCGCTTATC GTTTCCAGCA CTTCTCCGAT ATCGATCTTA 
AACAACCAGG ACCCGTCGCT GAACAGTCTA TCAGTGATGA ACGCCACCGA CCCCAGGAAC 
AACATGGACA TGACGGTGGG CTCCCGCCAG CAGAGCGCTC AGGGCTGGAT CAACACGTAC 
CCCCTGTCTA GCGGCATGTC CACCACCTCT AAGAAATCAG GCATGTCCAA AAAAAGCAAC 
AGAGGAACCC AGCTGCACAA GTACTACATG AAACGCAGGA CGTTGCTCTT GGCCTTGCTG 
GCCAGCGAGA TCGAGAGACT GAGCACATGG TACAACCCGC TGTCCACACA GGAGCTGGCC 
ATTCCCACCG AGCAGTCTGT GGAAACCAGC ATCTCCAACT GGAGGTCCAA ATACATCAAC 
CTGACCGAGA AACAGTGGAA GGATAATGTC AATTTGGCCT GGAGTATCGC TCCTTACCTG 
GCCTTGCAGC TTCCGGCAAG GTTCAAAAAC GCAGACGCCA TTGTGGCAGA AGTGACTCGT 
CTAGTTCGGC TGGATCCTGG TGCTGTCAGC GATGTGCCAG AGGCAGTGAA GTTCCTGGTA 
ACGTGGCACA CCATTGATGC AGATTCTCCA GAGCTGAGCC ACATCTTGTG CTGGGCTCCT 
GCAGATCCTC CGACCGGCCT CTCGTATTTC TCCAGCATGT ATCCTCCTCA TCCGCTCACT 
GCTCAGTATG GAGTCAAAGT CCTTCGCTCT TTTCCTCCTG ATGCCATTTT GTTTTACATT 
CCTCAAATCG TTCAGGCGCT GCGCTACGAC AAGATGGGTT ACGTGCGCGA GTACATCCTG 
TGGGCCGCAC AGAAGTCGCA GCTCCTCGCA CATCAGTTCA TATGGAACAT GAAGACCAAC 
ATCTTTCTGG ATGAAGAGGG ACATCAGAAA GACCCGGACA TCGGTGAGCT TCTGGAGCAG 
ATGGTGGAAG AGATCACGCA CTCTCTGTCA GGTCCAGCTA AAGACTTCTA TCAGCGCGAG 
TTTGACTTCT TCAACAAGAT CACCAACGTC TCCGCCATCA TCAAGCCTGT TCCCAAAGGA 
GATGAGAGGA AGAGAGCGTG TCTGAAGGCG CTGTCAGAAA TCAAAGTTCA GCCAGGATGT 
TATTTGCCAA GCAATCCAGA AGCAATCGTC CTTGATATCG ACTACAAGTC AGGAACGCCC 
ATGCAGAGCG CTGCCAAAGC CCCTTATCTG GCCAAATTCA AAGTTAAGAG GTGTGGCGTT 
AGTGAGCTTG AAAAAGAGGG TCTGCGCTGT CCGTCCGACT CTCAGGATGA AGGAGATGAG 
AGCTCACTGA CGGCTCAAAG GGTCTGCTGG CAGGCGGCCA TCTTTAAAGT GGGCGATGAT 
TGCAGACAGG ACATGCTCGC GCTTCAGATC ATTGGCCTGT TCAAGAACAT CTTTCAGCTG 
GTGGGTTTGG ATCTGTTTGT GTTCCCGTAC CGGGTTGTGG CCACAGCTCC AGGATGTGGC 
GTGATTGAAT GTATTCCGGA CTGTAAATCC CGGGATCAGC TCGGCCGGCA GACTGACTTC 
GGCATGTACG ACTACTTCAG GAACCAATAT GGCGACGAAT CCACTCTGGC TTTCCAGAAG 
GCTCGGTATA ACTTCATTCG CAGTATGGCT GCTTACAGTC TGCTGCTCTT TCTGTTACAA 
ATCAAAGACC GACACAACGG CAACATCATG CTGGACAGCA AAGGCCATCT CATTCACATC 
GACTTTGGCT TCATGTTTGA GAGTTCTCCT GGTGGGAATC TGGGCTGGGA GCCTGACATC 
AAGCTGACCG ATGAGATGGT GATGATCATG GGAGGCAAGA TGGAGGCTAC ACCATTTAAA 
TGGTTCATGG AGATGTGTGT GCGCGGATAC CTGGCTGTAA GGCCCTACAT GGATGCTGTC 
GTGTCATTGG TGACGTTGAT GCTGGACACG GGGTTGCCTT GCTTCAGAGG ACAGACGATC 
AAACTCCTCA AGCAACGGTT CAACCCAAAC ATGAGCGAGA AGGAAGCTGC GGCCTTCATG 
ATCAAAGTCA TCGAGCGCTG CTTCCTCAGT AGCAGGAGCA AAACCTACGA CATGCTGCAA 
TACTACCAGA ACCAAATCCC ATACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303356.1
CDS	116..6502
Translation	MSSRGTSRGFYFNTVLSLARSLAAHRPAPIDKVQKLQCMCPVDIRGVFQLDERRRDAVIALGIFLVESELQHKDAIVPYLLVLLKGLPRVQWIEESSERKGREALPIAENFSFCLVTVLSDVAQMDESMRIQILEAVMNLIQVLQETCQNIEGQDKEYLCKYTVPCLLGVVRAFGRYSNTEEPLLSKLFPRIVPQPPGTGDGAEVTRRRSFNDFRSILPSSLLTVCQSDTLRRKTSSTSSISQQASPERAGLPPSSPSDPSAPYFEAASYLPDGSAVDPDYYFSTISSSFSVSPLFTGSNNKEFTVPLDLLRQLLQMVEQFVSESFMKTLDQAMTEVLEINPTAYLYYKTFSDPLYVAIFKMLRDSLYYMKDLQVSFVKEVHDFVLEQFSSSQSELQRILHDVEYLHSELNPLKLRCQANAACVDLMVWAVTEEQGAENLCTKLSEKLQSKTSSKVIIAHMPLLICCLQGLGRLCERFPVVAHSVTMSLRDFLVVPSPVLVKLYKYHSQYTTGAGEIKIHVTNEHSQSTFSSHSSKKSQPSMYEQLRDISINNICRCLKAGLTMDSVIVEAFLASLSNRLYISQENDKDAHLIPDHTIRALGHIAVALRDTPRVMEPILQILQQKFCQPPSQLDVLIIDQLGCMVITGNQYIYQEVWNLFQQISVKASSMVYSTKDYKDHGYRHCSLAVINALANIAANLQTEQMVDELLVNLLELFVQLGLEGKRASERASEKGPALKASSSAGNLGVLIPVIAVLTKRLPAIKEAKPRLQKLFRDFWLYSVVMGFAVEGSGLWPEEWYEGVCEIATKSPLLTFPSGEPLRSELQYNSALKNDTVTPAELNDLRGTIINLLDPPPEVAALINKLDFAMSTYLLSVYRLEYMRVLRSQESDRFQVMFRYFEDRAIQKDKSGMMQCVIAVGDKVFDVFLQMMADKAKTKAHEEELESHAQFLLVNFNHIHKRIRRVADKYLSGLAETFPHLLWSGRVLRTMLDILQTLSLSLSADIHKDQPYYDIPDTPYRITVPDTYEARESIVKDFAARCGEILKEAMKWAPSVTKSHLQEYLNKHQNWVSGLSQHTGLAMATESILHFAGYNRQSTSLGTTQLTERPACVKKDYSNFMASLNLRNRYAGEVAGMIHFSEATRSTSDLNKLMIRQMNEALDGGQPEAFTEALFKMAALLISSREWDPLLLHHLCWSPLKMFTENGMETAIACWEWLLAGRTGVEVPFMREMAGAWQMTVDLKMGLFSDAQLEADPLAASEESQPIPCPPDVTPHYIWIEFLVQRFEIAKYSSADQVEIFGSLLQRSLSLNVGSPKSSLNRHVAAIGPRFRLLTLGLSLLHSDVVTNATIRNVLREKIYSTAFDYFSVASKFPTQGDKRLREDISIMIRFYASILSDKKYLGASLLVPPGELGANPPQSSLSAGIFGSLIVSSTSPISILNNQDPSLNSLSVMNATDPRNNMDMTVGSRQQSAQGWINTYPLSSGMSTTSKKSGMSKKSNRGTQLHKYYMKRRTLLLALLASEIERLSTWYNPLSTQELAIPTEQSVETSISNWRSKYINLTEKQWKDNVNLAWSIAPYLALQLPARFKNADAIVAEVTRLVRLDPGAVSDVPEAVKFLVTWHTIDADSPELSHILCWAPADPPTGLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGYVREYILWAAQKSQLLAHQFIWNMKTNIFLDEEGHQKDPDIGELLEQMVEEITHSLSGPAKDFYQREFDFFNKITNVSAIIKPVPKGDERKRACLKALSEIKVQPGCYLPSNPEAIVLDIDYKSGTPMQSAAKAPYLAKFKVKRCGVSELEKEGLRCPSDSQDEGDESSLTAQRVCWQAAIFKVGDDCRQDMLALQIIGLFKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPDCKSRDQLGRQTDFGMYDYFRNQYGDESTLAFQKARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDSKGHLIHIDFGFMFESSPGGNLGWEPDIKLTDEMVMIMGGKMEATPFKWFMEMCVRGYLAVRPYMDAVVSLVTLMLDTGLPCFRGQTIKLLKQRFNPNMSEKEAAAFMIKVIERCFLSSRSKTYDMLQYYQNQIPY

Target ORF information:

RefSeq Version	XM_009305081.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphatidylinositol 4-kinase, catalytic, alpha a (pi4kaa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305081.3

ORF Insert Sequence:

ATGTCGTCGA GAGGGACGTC GCGGGGTTTT TACTTTAACA CTGTGCTTTC TCTCGCGCGC 
TCACTGGCTG CTCATCGACC CGCTCCGATC GACAAGGTCC AGAAGCTACA ATGCATGTGT 
CCTGTGGACA TCCGTGGTGT TTTCCAGCTG GACGAGCGGA GGAGAGATGC TGTGATCGCA 
CTTGGGATCT TCCTAGTGGA GTCTGAGTTG CAGCACAAGG ATGCTATTGT CCCGTACTTG 
TTGGTTTTGC TAAAGGGATT GCCCAGGGTG CAATGGATCG AGGAGAGCTC AGAAAGGAAA 
GGACGAGAAG CGCTTCCCAT TGCGGAGAAT TTCTCCTTCT GTTTGGTGAC GGTGCTCTCA 
GACGTGGCCC AGATGGATGA ATCCATGCGC ATCCAGATTT TGGAGGCTGT GATGAACCTC 
ATTCAGGTGC TACAGGAAAC ATGTCAGAAC ATCGAGGGCC AAGATAAAGA ATACCTGTGT 
AAATACACCG TGCCCTGTCT GCTCGGTGTG GTGCGAGCGT TTGGCCGCTA CAGCAACACA 
GAGGAGCCGC TGTTGTCTAA ACTCTTCCCC CGGATCGTTC CTCAGCCTCC AGGCACCGGC 
GACGGCGCAG AGGTGACCCG ACGGAGATCT TTTAATGATT TCCGCTCCAT CCTGCCCAGC 
TCACTCCTCA CCGTTTGCCA AAGTGACACT TTACGCCGCA AAACCAGCAG CACTTCCAGC 
ATCTCACAAC AGGCCAGTCC AGAACGTGCT GGACTTCCTC CGAGTTCTCC CTCTGACCCT 
TCTGCTCCTT ATTTCGAAGC TGCCTCGTAT CTGCCCGACG GCAGTGCTGT GGACCCGGAC 
TACTATTTCT CCACCATCAG CTCCAGCTTC TCAGTCTCTC CGCTCTTCAC AGGCAGCAAC 
AATAAAGAGT TTACCGTACC GCTGGATTTA CTGAGGCAGC TTCTGCAGAT GGTCGAGCAG 
TTTGTGTCAG AATCCTTCAT GAAGACACTG GATCAGGCCA TGACTGAAGT TCTGGAGATC 
AATCCCACGG CTTATCTGTA CTACAAGACT TTCAGTGACC CGCTGTACGT GGCCATCTTT 
AAAATGCTGA GGGACTCGTT GTATTATATG AAAGATCTGC AGGTGTCGTT TGTGAAGGAG 
GTCCATGACT TTGTTCTGGA GCAGTTCAGC AGCAGTCAGT CTGAGCTGCA GAGGATCCTG 
CACGATGTGG AGTATCTGCA CAGTGAACTG AACCCGCTTA AGCTGCGCTG TCAGGCCAAC 
GCCGCCTGTG TGGACCTCAT GGTGTGGGCC GTCACGGAAG AACAGGGCGC TGAAAATCTC 
TGCACTAAAC TGTCAGAGAA GCTCCAGTCC AAGACGTCCA GCAAAGTCAT CATCGCCCAC 
ATGCCTCTGC TCATCTGCTG CTTACAGGGT CTCGGCCGGC TCTGTGAGCG TTTTCCTGTG 
GTGGCTCACT CTGTGACCAT GTCTCTACGA GATTTTCTGG TGGTTCCTTC ACCTGTACTA 
GTGAAGCTCT ACAAGTACCA CAGTCAGTAC ACCACAGGAG CAGGTGAGAT CAAGATTCAT 
GTCACCAACG AGCATTCCCA GTCCACCTTC AGCTCGCACT CCAGCAAGAA GAGCCAGCCG 
TCCATGTATG AGCAGCTCCG AGACATTTCC ATCAACAATA TATGCAGGTG TTTAAAAGCA 
GGATTGACCA TGGATTCAGT CATCGTTGAA GCTTTCCTCG CCAGTCTTTC CAACCGTCTG 
TACATTTCGC AGGAAAATGA CAAGGATGCC CATCTAATCC CGGACCACAC CATACGGGCG 
TTAGGACACA TCGCTGTGGC TCTGAGAGAC ACTCCACGAG TGATGGAGCC CATCTTACAG 
ATCCTGCAGC AGAAGTTCTG CCAGCCGCCG TCTCAGCTCG ACGTGCTCAT CATAGATCAG 
CTGGGCTGTA TGGTCATCAC TGGAAACCAA TATATTTATC AGGAAGTGTG GAACCTGTTC 
CAGCAGATCA GCGTGAAGGC CAGCTCCATG GTTTACTCCA CCAAAGACTA CAAGGATCAT 
GGATACAGGC ACTGTTCTCT GGCCGTGATC AATGCTCTGG CCAACATCGC AGCCAACCTG 
CAGACGGAGC AGATGGTGGA CGAGCTGCTG GTCAATCTGC TGGAACTGTT TGTGCAGCTG 
GGGCTGGAGG GCAAACGGGC CAGTGAAAGA GCCAGCGAGA AGGGCCCGGC CCTCAAGGCG 
TCAAGCAGCG CTGGTAATCT CGGAGTTCTC ATTCCTGTCA TTGCTGTGCT AACCAAACGT 
TTGCCGGCCA TAAAAGAAGC CAAACCGAGG CTGCAGAAGC TGTTCAGAGA TTTCTGGCTG 
TATTCGGTTG TGATGGGCTT CGCTGTTGAG GGCTCAGGTC TGTGGCCGGA GGAGTGGTAT 
GAGGGAGTGT GTGAGATTGC AACAAAATCC CCACTGCTGA CCTTCCCAAG CGGAGAACCG 
CTGCGCTCAG AGCTGCAGTA TAACTCGGCC CTGAAGAACG ACACTGTCAC GCCGGCTGAG 
CTGAATGATT TGAGGGGAAC CATCATTAAC CTGCTGGATC CTCCGCCGGA GGTTGCTGCG 
CTCATTAATA AACTGGACTT TGCCATGTCC ACCTACCTGC TGTCCGTCTA CCGGCTGGAA 
TACATGAGAG TGTTACGATC TCAGGAGTCT GACCGATTCC AAGTGATGTT TCGCTATTTT 
GAAGACCGAG CGATTCAGAA GGATAAATCA GGAATGATGC AGTGTGTCAT CGCAGTAGGT 
GACAAGGTCT TCGATGTCTT TCTTCAGATG ATGGCTGACA AGGCAAAGAC CAAAGCCCAT 
GAGGAGGAGC TGGAAAGTCA TGCTCAGTTT CTATTGGTCA ACTTCAACCA CATTCATAAG 
AGAATCCGAA GAGTTGCTGA CAAATATCTG TCAGGACTCG CTGAGACATT TCCACACTTG 
CTGTGGAGCG GCCGTGTTCT GAGGACCATG CTGGATATAT TACAGACTCT GTCTCTTTCG 
CTCAGTGCTG ATATCCACAA AGACCAACCA TATTACGACA TACCAGACAC ACCATATCGC 
ATCACAGTGC CGGACACGTA TGAGGCCAGA GAGAGCATAG TGAAGGACTT TGCCGCCCGC 
TGTGGAGAAA TCCTGAAAGA GGCCATGAAA TGGGCGCCAT CGGTCACCAA ATCACACCTA 
CAGGAATACC TGAATAAGCA TCAGAACTGG GTGTCGGGTC TGTCTCAGCA CACAGGGCTG 
GCGATGGCCA CAGAGAGCAT CCTGCACTTC GCCGGATACA ACCGTCAGAG CACCAGTCTC 
GGTACCACTC AGCTGACCGA GAGACCCGCA TGCGTGAAGA AAGACTACTC CAACTTCATG 
GCCTCCCTCA ACCTGAGAAA CCGCTACGCT GGAGAGGTCG CAGGGATGAT CCATTTCTCC 
GAGGCCACGC GGAGCACATC AGACCTGAAT AAACTGATGA TCCGACAGAT GAATGAAGCT 
CTGGATGGAG GACAGCCCGA AGCCTTCACC GAAGCCCTGT TCAAGATGGC AGCGCTGTTG 
ATCAGCTCCA GAGAATGGGA CCCTCTGCTG TTGCATCACC TGTGCTGGTC TCCATTAAAG 
ATGTTTACTG AGAATGGCAT GGAAACGGCT ATAGCCTGCT GGGAATGGCT TCTGGCTGGA 
AGAACAGGAG TTGAAGTCCC GTTCATGCGT GAGATGGCCG GCGCGTGGCA GATGACGGTG 
GATCTGAAGA TGGGGCTGTT TTCTGATGCC CAGCTGGAGG CCGATCCCCT GGCGGCTTCC 
GAGGAAAGCC AACCCATCCC CTGCCCGCCC GACGTCACGC CACACTACAT CTGGATTGAG 
TTTCTGGTGC AGCGGTTTGA GATCGCCAAG TACAGCAGCG CCGATCAGGT GGAGATTTTC 
GGCAGTCTTC TGCAGAGGTC CCTCTCTCTG AATGTCGGCA GCCCAAAAAG CAGTCTGAAC 
CGCCATGTGG CAGCCATCGG ACCTCGCTTC AGGTTGTTGA CGCTCGGACT TTCTCTCCTG 
CATTCTGACG TAGTGACCAA TGCCACCATC CGTAACGTCC TAAGGGAAAA GATTTATTCC 
ACTGCCTTCG ATTACTTCAG TGTGGCATCA AAGTTCCCCA CTCAGGGAGA CAAGCGTCTG 
CGGGAGGACA TCAGCATCAT GATCCGCTTC TACGCCAGCA TCCTCTCCGA CAAGAAGTAC 
CTAGGTGCTA GCCTGCTCGT GCCACCTGGT GAGCTCGGTG CCAATCCCCC TCAAAGCTCC 
CTATCTGCCG GGATTTTCGG ATCGCTTATC GTTTCCAGCA CTTCTCCGAT ATCGATCTTA 
AACAACCAGG ACCCGTCGCT GAACAGTCTA TCAGTGATGA ACGCCACCGA CCCCAGGAAC 
AACATGGACA TGACGGTGGG CTCCCGCCAG CAGAGCGCTC AGGGCTGGAT CAACACGTAC 
CCCCTGTCTA GCGGCATGTC CACCACCTCT AAGAAATCAG GCATGTCCAA AAAAAGCAAC 
AGAGGAACCC AGCTGCACAA GTACTACATG AAACGCAGGA CGTTGCTCTT GGCCTTGCTG 
GCCAGCGAGA TCGAGAGACT GAGCACATGG TACAACCCGC TGTCCACACA GGAGCTGGCC 
ATTCCCACCG AGCAGTCTGT GGAAACCAGC ATCTCCAACT GGAGGTCCAA ATACATCAAC 
CTGACCGAGA AACAGTGGAA GGATAATGTC AATTTGGCCT GGAGTATCGC TCCTTACCTG 
GCCTTGCAGC TTCCGGCAAG GTTCAAAAAC GCAGACGCCA TTGTGGCAGA AGTGACTCGT 
CTAGTTCGGC TGGATCCTGG TGCTGTCAGC GATGTGCCAG AGGCAGTGAA GTTCCTGGTA 
ACGTGGCACA CCATTGATGC AGATTCTCCA GAGCTGAGCC ACATCTTGTG CTGGGCTCCT 
GCAGATCCTC CGACCGGCCT CTCGTATTTC TCCAGCATGT ATCCTCCTCA TCCGCTCACT 
GCTCAGTATG GAGTCAAAGT CCTTCGCTCT TTTCCTCCTG ATGCCATTTT GTTTTACATT 
CCTCAAATCG TTCAGGCGCT GCGCTACGAC AAGATGGGTT ACGTGCGCGA GTACATCCTG 
TGGGCCGCAC AGAAGTCGCA GCTCCTCGCA CATCAGTTCA TATGGAACAT GAAGACCAAC 
ATCTTTCTGG ATGAAGAGGG ACATCAGAAA GACCCGGACA TCGGTGAGCT TCTGGAGCAG 
ATGGTGGAAG AGATCACGCA CTCTCTGTCA GGTCCAGCTA AAGACTTCTA TCAGCGCGAG 
TTTGACTTCT TCAACAAGAT CACCAACGTC TCCGCCATCA TCAAGCCTGT TCCCAAAGGA 
GATGAGAGGA AGAGAGCGTG TCTGAAGGCG CTGTCAGAAA TCAAAGTTCA GCCAGGATGT 
TATTTGCCAA GCAATCCAGA AGCAATCGTC CTTGATATCG ACTACAAGTC AGGAACGCCC 
ATGCAGAGCG CTGCCAAAGC CCCTTATCTG GCCAAATTCA AAGTTAAGAG GTGTGGCGTT 
AGTGAGCTTG AAAAAGAGGG TCTGCGCTGT CCGTCCGACT CTCAGGATGA AGGAGATGAG 
AGCTCACTGA CGGCTCAAAG GGTCTGCTGG CAGGCGGCCA TCTTTAAAGT GGGCGATGAT 
TGCAGACAGG ACATGCTCGC GCTTCAGATC ATTGGCCTGT TCAAGAACAT CTTTCAGCTG 
GTGGGTTTGG ATCTGTTTGT GTTCCCGTAC CGGGTTGTGG CCACAGCTCC AGGATGTGGC 
GTGATTGAAT GTATTCCGGA CTGTAAATCC CGGGATCAGC TCGGCCGGCA GACTGACTTC 
GGCATGTACG ACTACTTCAG GAACCAATAT GGCGACGAAT CCACTCTGGC TTTCCAGAAG 
GCTCGGTATA ACTTCATTCG CAGTATGGCT GCTTACAGTC TGCTGCTCTT TCTGTTACAA 
ATCAAAGACC GACACAACGG CAACATCATG CTGGACAGCA AAGGCCATCT CATTCACATC 
GACTTTGGCT TCATGTTTGA GAGTTCTCCT GGTGGGAATC TGGGCTGGGA GCCTGACATC 
AAGCTGACCG ATGAGATGGT GATGATCATG GGAGGCAAGA TGGAGGCTAC ACCATTTAAA 
TGGTTCATGG AGATGTGTGT GCGCGGATAC CTGGCTGTAA GGCCCTACAT GGATGCTGTC 
GTGTCATTGG TGACGTTGAT GCTGGACACG GGGTTGCCTT GCTTCAGAGG ACAGACGATC 
AAACTCCTCA AGCAACGGTT CAACCCAAAC ATGAGCGAGA AGGAAGCTGC GGCCTTCATG 
ATCAAAGTCA TCGAGCGCTG CTTCCTCAGT AGCAGGAGCA AAACCTACGA CATGCTGCAA 
TACTACCAGA ACCAAATCCC ATACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pi4kaa ( XM_009305081.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303356.1 Danio rerio phosphatidylinositol 4-kinase, catalytic, alpha a (pi4kaa), transcript variant X1, mRNA.

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CloneID	ODa42467
Clone ID Related Accession (Same CDS sequence)	XM_009305081.3 , XM_009305081.2
Accession Version	XM_009305081.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	phosphatidylinositol 4-kinase alpha isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305081.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCGA GAGGGACGTC GCGGGGTTTT TACTTTAACA CTGTGCTTTC TCTCGCGCGC 
TCACTGGCTG CTCATCGACC CGCTCCGATC GACAAGGTCC AGAAGCTACA ATGCATGTGT 
CCTGTGGACA TCCGTGGTGT TTTCCAGCTG GACGAGCGGA GGAGAGATGC TGTGATCGCA 
CTTGGGATCT TCCTAGTGGA GTCTGAGTTG CAGCACAAGG ATGCTATTGT CCCGTACTTG 
TTGGTTTTGC TAAAGGGATT GCCCAGGGTG CAATGGATCG AGGAGAGCTC AGAAAGGAAA 
GGACGAGAAG CGCTTCCCAT TGCGGAGAAT TTCTCCTTCT GTTTGGTGAC GGTGCTCTCA 
GACGTGGCCC AGATGGATGA ATCCATGCGC ATCCAGATTT TGGAGGCTGT GATGAACCTC 
ATTCAGGTGC TACAGGAAAC ATGTCAGAAC ATCGAGGGCC AAGATAAAGA ATACCTGTGT 
AAATACACCG TGCCCTGTCT GCTCGGTGTG GTGCGAGCGT TTGGCCGCTA CAGCAACACA 
GAGGAGCCGC TGTTGTCTAA ACTCTTCCCC CGGATCGTTC CTCAGCCTCC AGGCACCGGC 
GACGGCGCAG AGGTGACCCG ACGGAGATCT TTTAATGATT TCCGCTCCAT CCTGCCCAGC 
TCACTCCTCA CCGTTTGCCA AAGTGACACT TTACGCCGCA AAACCAGCAG CACTTCCAGC 
ATCTCACAAC AGGCCAGTCC AGAACGTGCT GGACTTCCTC CGAGTTCTCC CTCTGACCCT 
TCTGCTCCTT ATTTCGAAGC TGCCTCGTAT CTGCCCGACG GCAGTGCTGT GGACCCGGAC 
TACTATTTCT CCACCATCAG CTCCAGCTTC TCAGTCTCTC CGCTCTTCAC AGGCAGCAAC 
AATAAAGAGT TTACCGTACC GCTGGATTTA CTGAGGCAGC TTCTGCAGAT GGTCGAGCAG 
TTTGTGTCAG AATCCTTCAT GAAGACACTG GATCAGGCCA TGACTGAAGT TCTGGAGATC 
AATCCCACGG CTTATCTGTA CTACAAGACT TTCAGTGACC CGCTGTACGT GGCCATCTTT 
AAAATGCTGA GGGACTCGTT GTATTATATG AAAGATCTGC AGGTGTCGTT TGTGAAGGAG 
GTCCATGACT TTGTTCTGGA GCAGTTCAGC AGCAGTCAGT CTGAGCTGCA GAGGATCCTG 
CACGATGTGG AGTATCTGCA CAGTGAACTG AACCCGCTTA AGCTGCGCTG TCAGGCCAAC 
GCCGCCTGTG TGGACCTCAT GGTGTGGGCC GTCACGGAAG AACAGGGCGC TGAAAATCTC 
TGCACTAAAC TGTCAGAGAA GCTCCAGTCC AAGACGTCCA GCAAAGTCAT CATCGCCCAC 
ATGCCTCTGC TCATCTGCTG CTTACAGGGT CTCGGCCGGC TCTGTGAGCG TTTTCCTGTG 
GTGGCTCACT CTGTGACCAT GTCTCTACGA GATTTTCTGG TGGTTCCTTC ACCTGTACTA 
GTGAAGCTCT ACAAGTACCA CAGTCAGTAC ACCACAGGAG CAGGTGAGAT CAAGATTCAT 
GTCACCAACG AGCATTCCCA GTCCACCTTC AGCTCGCACT CCAGCAAGAA GAGCCAGCCG 
TCCATGTATG AGCAGCTCCG AGACATTTCC ATCAACAATA TATGCAGGTG TTTAAAAGCA 
GGATTGACCA TGGATTCAGT CATCGTTGAA GCTTTCCTCG CCAGTCTTTC CAACCGTCTG 
TACATTTCGC AGGAAAATGA CAAGGATGCC CATCTAATCC CGGACCACAC CATACGGGCG 
TTAGGACACA TCGCTGTGGC TCTGAGAGAC ACTCCACGAG TGATGGAGCC CATCTTACAG 
ATCCTGCAGC AGAAGTTCTG CCAGCCGCCG TCTCAGCTCG ACGTGCTCAT CATAGATCAG 
CTGGGCTGTA TGGTCATCAC TGGAAACCAA TATATTTATC AGGAAGTGTG GAACCTGTTC 
CAGCAGATCA GCGTGAAGGC CAGCTCCATG GTTTACTCCA CCAAAGACTA CAAGGATCAT 
GGATACAGGC ACTGTTCTCT GGCCGTGATC AATGCTCTGG CCAACATCGC AGCCAACCTG 
CAGACGGAGC AGATGGTGGA CGAGCTGCTG GTCAATCTGC TGGAACTGTT TGTGCAGCTG 
GGGCTGGAGG GCAAACGGGC CAGTGAAAGA GCCAGCGAGA AGGGCCCGGC CCTCAAGGCG 
TCAAGCAGCG CTGGTAATCT CGGAGTTCTC ATTCCTGTCA TTGCTGTGCT AACCAAACGT 
TTGCCGGCCA TAAAAGAAGC CAAACCGAGG CTGCAGAAGC TGTTCAGAGA TTTCTGGCTG 
TATTCGGTTG TGATGGGCTT CGCTGTTGAG GGCTCAGGTC TGTGGCCGGA GGAGTGGTAT 
GAGGGAGTGT GTGAGATTGC AACAAAATCC CCACTGCTGA CCTTCCCAAG CGGAGAACCG 
CTGCGCTCAG AGCTGCAGTA TAACTCGGCC CTGAAGAACG ACACTGTCAC GCCGGCTGAG 
CTGAATGATT TGAGGGGAAC CATCATTAAC CTGCTGGATC CTCCGCCGGA GGTTGCTGCG 
CTCATTAATA AACTGGACTT TGCCATGTCC ACCTACCTGC TGTCCGTCTA CCGGCTGGAA 
TACATGAGAG TGTTACGATC TCAGGAGTCT GACCGATTCC AAGTGATGTT TCGCTATTTT 
GAAGACCGAG CGATTCAGAA GGATAAATCA GGAATGATGC AGTGTGTCAT CGCAGTAGGT 
GACAAGGTCT TCGATGTCTT TCTTCAGATG ATGGCTGACA AGGCAAAGAC CAAAGCCCAT 
GAGGAGGAGC TGGAAAGTCA TGCTCAGTTT CTATTGGTCA ACTTCAACCA CATTCATAAG 
AGAATCCGAA GAGTTGCTGA CAAATATCTG TCAGGACTCG CTGAGACATT TCCACACTTG 
CTGTGGAGCG GCCGTGTTCT GAGGACCATG CTGGATATAT TACAGACTCT GTCTCTTTCG 
CTCAGTGCTG ATATCCACAA AGACCAACCA TATTACGACA TACCAGACAC ACCATATCGC 
ATCACAGTGC CGGACACGTA TGAGGCCAGA GAGAGCATAG TGAAGGACTT TGCCGCCCGC 
TGTGGAGAAA TCCTGAAAGA GGCCATGAAA TGGGCGCCAT CGGTCACCAA ATCACACCTA 
CAGGAATACC TGAATAAGCA TCAGAACTGG GTGTCGGGTC TGTCTCAGCA CACAGGGCTG 
GCGATGGCCA CAGAGAGCAT CCTGCACTTC GCCGGATACA ACCGTCAGAG CACCAGTCTC 
GGTACCACTC AGCTGACCGA GAGACCCGCA TGCGTGAAGA AAGACTACTC CAACTTCATG 
GCCTCCCTCA ACCTGAGAAA CCGCTACGCT GGAGAGGTCG CAGGGATGAT CCATTTCTCC 
GAGGCCACGC GGAGCACATC AGACCTGAAT AAACTGATGA TCCGACAGAT GAATGAAGCT 
CTGGATGGAG GACAGCCCGA AGCCTTCACC GAAGCCCTGT TCAAGATGGC AGCGCTGTTG 
ATCAGCTCCA GAGAATGGGA CCCTCTGCTG TTGCATCACC TGTGCTGGTC TCCATTAAAG 
ATGTTTACTG AGAATGGCAT GGAAACGGCT ATAGCCTGCT GGGAATGGCT TCTGGCTGGA 
AGAACAGGAG TTGAAGTCCC GTTCATGCGT GAGATGGCCG GCGCGTGGCA GATGACGGTG 
GATCTGAAGA TGGGGCTGTT TTCTGATGCC CAGCTGGAGG CCGATCCCCT GGCGGCTTCC 
GAGGAAAGCC AACCCATCCC CTGCCCGCCC GACGTCACGC CACACTACAT CTGGATTGAG 
TTTCTGGTGC AGCGGTTTGA GATCGCCAAG TACAGCAGCG CCGATCAGGT GGAGATTTTC 
GGCAGTCTTC TGCAGAGGTC CCTCTCTCTG AATGTCGGCA GCCCAAAAAG CAGTCTGAAC 
CGCCATGTGG CAGCCATCGG ACCTCGCTTC AGGTTGTTGA CGCTCGGACT TTCTCTCCTG 
CATTCTGACG TAGTGACCAA TGCCACCATC CGTAACGTCC TAAGGGAAAA GATTTATTCC 
ACTGCCTTCG ATTACTTCAG TGTGGCATCA AAGTTCCCCA CTCAGGGAGA CAAGCGTCTG 
CGGGAGGACA TCAGCATCAT GATCCGCTTC TACGCCAGCA TCCTCTCCGA CAAGAAGTAC 
CTAGGTGCTA GCCTGCTCGT GCCACCTGGT GAGCTCGGTG CCAATCCCCC TCAAAGCTCC 
CTATCTGCCG GGATTTTCGG ATCGCTTATC GTTTCCAGCA CTTCTCCGAT ATCGATCTTA 
AACAACCAGG ACCCGTCGCT GAACAGTCTA TCAGTGATGA ACGCCACCGA CCCCAGGAAC 
AACATGGACA TGACGGTGGG CTCCCGCCAG CAGAGCGCTC AGGGCTGGAT CAACACGTAC 
CCCCTGTCTA GCGGCATGTC CACCACCTCT AAGAAATCAG GCATGTCCAA AAAAAGCAAC 
AGAGGAACCC AGCTGCACAA GTACTACATG AAACGCAGGA CGTTGCTCTT GGCCTTGCTG 
GCCAGCGAGA TCGAGAGACT GAGCACATGG TACAACCCGC TGTCCACACA GGAGCTGGCC 
ATTCCCACCG AGCAGTCTGT GGAAACCAGC ATCTCCAACT GGAGGTCCAA ATACATCAAC 
CTGACCGAGA AACAGTGGAA GGATAATGTC AATTTGGCCT GGAGTATCGC TCCTTACCTG 
GCCTTGCAGC TTCCGGCAAG GTTCAAAAAC GCAGACGCCA TTGTGGCAGA AGTGACTCGT 
CTAGTTCGGC TGGATCCTGG TGCTGTCAGC GATGTGCCAG AGGCAGTGAA GTTCCTGGTA 
ACGTGGCACA CCATTGATGC AGATTCTCCA GAGCTGAGCC ACATCTTGTG CTGGGCTCCT 
GCAGATCCTC CGACCGGCCT CTCGTATTTC TCCAGCATGT ATCCTCCTCA TCCGCTCACT 
GCTCAGTATG GAGTCAAAGT CCTTCGCTCT TTTCCTCCTG ATGCCATTTT GTTTTACATT 
CCTCAAATCG TTCAGGCGCT GCGCTACGAC AAGATGGGTT ACGTGCGCGA GTACATCCTG 
TGGGCCGCAC AGAAGTCGCA GCTCCTCGCA CATCAGTTCA TATGGAACAT GAAGACCAAC 
ATCTTTCTGG ATGAAGAGGG ACATCAGAAA GACCCGGACA TCGGTGAGCT TCTGGAGCAG 
ATGGTGGAAG AGATCACGCA CTCTCTGTCA GGTCCAGCTA AAGACTTCTA TCAGCGCGAG 
TTTGACTTCT TCAACAAGAT CACCAACGTC TCCGCCATCA TCAAGCCTGT TCCCAAAGGA 
GATGAGAGGA AGAGAGCGTG TCTGAAGGCG CTGTCAGAAA TCAAAGTTCA GCCAGGATGT 
TATTTGCCAA GCAATCCAGA AGCAATCGTC CTTGATATCG ACTACAAGTC AGGAACGCCC 
ATGCAGAGCG CTGCCAAAGC CCCTTATCTG GCCAAATTCA AAGTTAAGAG GTGTGGCGTT 
AGTGAGCTTG AAAAAGAGGG TCTGCGCTGT CCGTCCGACT CTCAGGATGA AGGAGATGAG 
AGCTCACTGA CGGCTCAAAG GGTCTGCTGG CAGGCGGCCA TCTTTAAAGT GGGCGATGAT 
TGCAGACAGG ACATGCTCGC GCTTCAGATC ATTGGCCTGT TCAAGAACAT CTTTCAGCTG 
GTGGGTTTGG ATCTGTTTGT GTTCCCGTAC CGGGTTGTGG CCACAGCTCC AGGATGTGGC 
GTGATTGAAT GTATTCCGGA CTGTAAATCC CGGGATCAGC TCGGCCGGCA GACTGACTTC 
GGCATGTACG ACTACTTCAG GAACCAATAT GGCGACGAAT CCACTCTGGC TTTCCAGAAG 
GCTCGGTATA ACTTCATTCG CAGTATGGCT GCTTACAGTC TGCTGCTCTT TCTGTTACAA 
ATCAAAGACC GACACAACGG CAACATCATG CTGGACAGCA AAGGCCATCT CATTCACATC 
GACTTTGGCT TCATGTTTGA GAGTTCTCCT GGTGGGAATC TGGGCTGGGA GCCTGACATC 
AAGCTGACCG ATGAGATGGT GATGATCATG GGAGGCAAGA TGGAGGCTAC ACCATTTAAA 
TGGTTCATGG AGATGTGTGT GCGCGGATAC CTGGCTGTAA GGCCCTACAT GGATGCTGTC 
GTGTCATTGG TGACGTTGAT GCTGGACACG GGGTTGCCTT GCTTCAGAGG ACAGACGATC 
AAACTCCTCA AGCAACGGTT CAACCCAAAC ATGAGCGAGA AGGAAGCTGC GGCCTTCATG 
ATCAAAGTCA TCGAGCGCTG CTTCCTCAGT AGCAGGAGCA AAACCTACGA CATGCTGCAA 
TACTACCAGA ACCAAATCCC ATACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303356.1
CDS	99..6485
Translation	MSSRGTSRGFYFNTVLSLARSLAAHRPAPIDKVQKLQCMCPVDIRGVFQLDERRRDAVIALGIFLVESELQHKDAIVPYLLVLLKGLPRVQWIEESSERKGREALPIAENFSFCLVTVLSDVAQMDESMRIQILEAVMNLIQVLQETCQNIEGQDKEYLCKYTVPCLLGVVRAFGRYSNTEEPLLSKLFPRIVPQPPGTGDGAEVTRRRSFNDFRSILPSSLLTVCQSDTLRRKTSSTSSISQQASPERAGLPPSSPSDPSAPYFEAASYLPDGSAVDPDYYFSTISSSFSVSPLFTGSNNKEFTVPLDLLRQLLQMVEQFVSESFMKTLDQAMTEVLEINPTAYLYYKTFSDPLYVAIFKMLRDSLYYMKDLQVSFVKEVHDFVLEQFSSSQSELQRILHDVEYLHSELNPLKLRCQANAACVDLMVWAVTEEQGAENLCTKLSEKLQSKTSSKVIIAHMPLLICCLQGLGRLCERFPVVAHSVTMSLRDFLVVPSPVLVKLYKYHSQYTTGAGEIKIHVTNEHSQSTFSSHSSKKSQPSMYEQLRDISINNICRCLKAGLTMDSVIVEAFLASLSNRLYISQENDKDAHLIPDHTIRALGHIAVALRDTPRVMEPILQILQQKFCQPPSQLDVLIIDQLGCMVITGNQYIYQEVWNLFQQISVKASSMVYSTKDYKDHGYRHCSLAVINALANIAANLQTEQMVDELLVNLLELFVQLGLEGKRASERASEKGPALKASSSAGNLGVLIPVIAVLTKRLPAIKEAKPRLQKLFRDFWLYSVVMGFAVEGSGLWPEEWYEGVCEIATKSPLLTFPSGEPLRSELQYNSALKNDTVTPAELNDLRGTIINLLDPPPEVAALINKLDFAMSTYLLSVYRLEYMRVLRSQESDRFQVMFRYFEDRAIQKDKSGMMQCVIAVGDKVFDVFLQMMADKAKTKAHEEELESHAQFLLVNFNHIHKRIRRVADKYLSGLAETFPHLLWSGRVLRTMLDILQTLSLSLSADIHKDQPYYDIPDTPYRITVPDTYEARESIVKDFAARCGEILKEAMKWAPSVTKSHLQEYLNKHQNWVSGLSQHTGLAMATESILHFAGYNRQSTSLGTTQLTERPACVKKDYSNFMASLNLRNRYAGEVAGMIHFSEATRSTSDLNKLMIRQMNEALDGGQPEAFTEALFKMAALLISSREWDPLLLHHLCWSPLKMFTENGMETAIACWEWLLAGRTGVEVPFMREMAGAWQMTVDLKMGLFSDAQLEADPLAASEESQPIPCPPDVTPHYIWIEFLVQRFEIAKYSSADQVEIFGSLLQRSLSLNVGSPKSSLNRHVAAIGPRFRLLTLGLSLLHSDVVTNATIRNVLREKIYSTAFDYFSVASKFPTQGDKRLREDISIMIRFYASILSDKKYLGASLLVPPGELGANPPQSSLSAGIFGSLIVSSTSPISILNNQDPSLNSLSVMNATDPRNNMDMTVGSRQQSAQGWINTYPLSSGMSTTSKKSGMSKKSNRGTQLHKYYMKRRTLLLALLASEIERLSTWYNPLSTQELAIPTEQSVETSISNWRSKYINLTEKQWKDNVNLAWSIAPYLALQLPARFKNADAIVAEVTRLVRLDPGAVSDVPEAVKFLVTWHTIDADSPELSHILCWAPADPPTGLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGYVREYILWAAQKSQLLAHQFIWNMKTNIFLDEEGHQKDPDIGELLEQMVEEITHSLSGPAKDFYQREFDFFNKITNVSAIIKPVPKGDERKRACLKALSEIKVQPGCYLPSNPEAIVLDIDYKSGTPMQSAAKAPYLAKFKVKRCGVSELEKEGLRCPSDSQDEGDESSLTAQRVCWQAAIFKVGDDCRQDMLALQIIGLFKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPDCKSRDQLGRQTDFGMYDYFRNQYGDESTLAFQKARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDSKGHLIHIDFGFMFESSPGGNLGWEPDIKLTDEMVMIMGGKMEATPFKWFMEMCVRGYLAVRPYMDAVVSLVTLMLDTGLPCFRGQTIKLLKQRFNPNMSEKEAAAFMIKVIERCFLSSRSKTYDMLQYYQNQIPY

Target ORF information:

RefSeq Version	XM_009305081.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio phosphatidylinositol 4-kinase, catalytic, alpha a (pi4kaa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305081.2

ORF Insert Sequence:

ATGTCGTCGA GAGGGACGTC GCGGGGTTTT TACTTTAACA CTGTGCTTTC TCTCGCGCGC 
TCACTGGCTG CTCATCGACC CGCTCCGATC GACAAGGTCC AGAAGCTACA ATGCATGTGT 
CCTGTGGACA TCCGTGGTGT TTTCCAGCTG GACGAGCGGA GGAGAGATGC TGTGATCGCA 
CTTGGGATCT TCCTAGTGGA GTCTGAGTTG CAGCACAAGG ATGCTATTGT CCCGTACTTG 
TTGGTTTTGC TAAAGGGATT GCCCAGGGTG CAATGGATCG AGGAGAGCTC AGAAAGGAAA 
GGACGAGAAG CGCTTCCCAT TGCGGAGAAT TTCTCCTTCT GTTTGGTGAC GGTGCTCTCA 
GACGTGGCCC AGATGGATGA ATCCATGCGC ATCCAGATTT TGGAGGCTGT GATGAACCTC 
ATTCAGGTGC TACAGGAAAC ATGTCAGAAC ATCGAGGGCC AAGATAAAGA ATACCTGTGT 
AAATACACCG TGCCCTGTCT GCTCGGTGTG GTGCGAGCGT TTGGCCGCTA CAGCAACACA 
GAGGAGCCGC TGTTGTCTAA ACTCTTCCCC CGGATCGTTC CTCAGCCTCC AGGCACCGGC 
GACGGCGCAG AGGTGACCCG ACGGAGATCT TTTAATGATT TCCGCTCCAT CCTGCCCAGC 
TCACTCCTCA CCGTTTGCCA AAGTGACACT TTACGCCGCA AAACCAGCAG CACTTCCAGC 
ATCTCACAAC AGGCCAGTCC AGAACGTGCT GGACTTCCTC CGAGTTCTCC CTCTGACCCT 
TCTGCTCCTT ATTTCGAAGC TGCCTCGTAT CTGCCCGACG GCAGTGCTGT GGACCCGGAC 
TACTATTTCT CCACCATCAG CTCCAGCTTC TCAGTCTCTC CGCTCTTCAC AGGCAGCAAC 
AATAAAGAGT TTACCGTACC GCTGGATTTA CTGAGGCAGC TTCTGCAGAT GGTCGAGCAG 
TTTGTGTCAG AATCCTTCAT GAAGACACTG GATCAGGCCA TGACTGAAGT TCTGGAGATC 
AATCCCACGG CTTATCTGTA CTACAAGACT TTCAGTGACC CGCTGTACGT GGCCATCTTT 
AAAATGCTGA GGGACTCGTT GTATTATATG AAAGATCTGC AGGTGTCGTT TGTGAAGGAG 
GTCCATGACT TTGTTCTGGA GCAGTTCAGC AGCAGTCAGT CTGAGCTGCA GAGGATCCTG 
CACGATGTGG AGTATCTGCA CAGTGAACTG AACCCGCTTA AGCTGCGCTG TCAGGCCAAC 
GCCGCCTGTG TGGACCTCAT GGTGTGGGCC GTCACGGAAG AACAGGGCGC TGAAAATCTC 
TGCACTAAAC TGTCAGAGAA GCTCCAGTCC AAGACGTCCA GCAAAGTCAT CATCGCCCAC 
ATGCCTCTGC TCATCTGCTG CTTACAGGGT CTCGGCCGGC TCTGTGAGCG TTTTCCTGTG 
GTGGCTCACT CTGTGACCAT GTCTCTACGA GATTTTCTGG TGGTTCCTTC ACCTGTACTA 
GTGAAGCTCT ACAAGTACCA CAGTCAGTAC ACCACAGGAG CAGGTGAGAT CAAGATTCAT 
GTCACCAACG AGCATTCCCA GTCCACCTTC AGCTCGCACT CCAGCAAGAA GAGCCAGCCG 
TCCATGTATG AGCAGCTCCG AGACATTTCC ATCAACAATA TATGCAGGTG TTTAAAAGCA 
GGATTGACCA TGGATTCAGT CATCGTTGAA GCTTTCCTCG CCAGTCTTTC CAACCGTCTG 
TACATTTCGC AGGAAAATGA CAAGGATGCC CATCTAATCC CGGACCACAC CATACGGGCG 
TTAGGACACA TCGCTGTGGC TCTGAGAGAC ACTCCACGAG TGATGGAGCC CATCTTACAG 
ATCCTGCAGC AGAAGTTCTG CCAGCCGCCG TCTCAGCTCG ACGTGCTCAT CATAGATCAG 
CTGGGCTGTA TGGTCATCAC TGGAAACCAA TATATTTATC AGGAAGTGTG GAACCTGTTC 
CAGCAGATCA GCGTGAAGGC CAGCTCCATG GTTTACTCCA CCAAAGACTA CAAGGATCAT 
GGATACAGGC ACTGTTCTCT GGCCGTGATC AATGCTCTGG CCAACATCGC AGCCAACCTG 
CAGACGGAGC AGATGGTGGA CGAGCTGCTG GTCAATCTGC TGGAACTGTT TGTGCAGCTG 
GGGCTGGAGG GCAAACGGGC CAGTGAAAGA GCCAGCGAGA AGGGCCCGGC CCTCAAGGCG 
TCAAGCAGCG CTGGTAATCT CGGAGTTCTC ATTCCTGTCA TTGCTGTGCT AACCAAACGT 
TTGCCGGCCA TAAAAGAAGC CAAACCGAGG CTGCAGAAGC TGTTCAGAGA TTTCTGGCTG 
TATTCGGTTG TGATGGGCTT CGCTGTTGAG GGCTCAGGTC TGTGGCCGGA GGAGTGGTAT 
GAGGGAGTGT GTGAGATTGC AACAAAATCC CCACTGCTGA CCTTCCCAAG CGGAGAACCG 
CTGCGCTCAG AGCTGCAGTA TAACTCGGCC CTGAAGAACG ACACTGTCAC GCCGGCTGAG 
CTGAATGATT TGAGGGGAAC CATCATTAAC CTGCTGGATC CTCCGCCGGA GGTTGCTGCG 
CTCATTAATA AACTGGACTT TGCCATGTCC ACCTACCTGC TGTCCGTCTA CCGGCTGGAA 
TACATGAGAG TGTTACGATC TCAGGAGTCT GACCGATTCC AAGTGATGTT TCGCTATTTT 
GAAGACCGAG CGATTCAGAA GGATAAATCA GGAATGATGC AGTGTGTCAT CGCAGTAGGT 
GACAAGGTCT TCGATGTCTT TCTTCAGATG ATGGCTGACA AGGCAAAGAC CAAAGCCCAT 
GAGGAGGAGC TGGAAAGTCA TGCTCAGTTT CTATTGGTCA ACTTCAACCA CATTCATAAG 
AGAATCCGAA GAGTTGCTGA CAAATATCTG TCAGGACTCG CTGAGACATT TCCACACTTG 
CTGTGGAGCG GCCGTGTTCT GAGGACCATG CTGGATATAT TACAGACTCT GTCTCTTTCG 
CTCAGTGCTG ATATCCACAA AGACCAACCA TATTACGACA TACCAGACAC ACCATATCGC 
ATCACAGTGC CGGACACGTA TGAGGCCAGA GAGAGCATAG TGAAGGACTT TGCCGCCCGC 
TGTGGAGAAA TCCTGAAAGA GGCCATGAAA TGGGCGCCAT CGGTCACCAA ATCACACCTA 
CAGGAATACC TGAATAAGCA TCAGAACTGG GTGTCGGGTC TGTCTCAGCA CACAGGGCTG 
GCGATGGCCA CAGAGAGCAT CCTGCACTTC GCCGGATACA ACCGTCAGAG CACCAGTCTC 
GGTACCACTC AGCTGACCGA GAGACCCGCA TGCGTGAAGA AAGACTACTC CAACTTCATG 
GCCTCCCTCA ACCTGAGAAA CCGCTACGCT GGAGAGGTCG CAGGGATGAT CCATTTCTCC 
GAGGCCACGC GGAGCACATC AGACCTGAAT AAACTGATGA TCCGACAGAT GAATGAAGCT 
CTGGATGGAG GACAGCCCGA AGCCTTCACC GAAGCCCTGT TCAAGATGGC AGCGCTGTTG 
ATCAGCTCCA GAGAATGGGA CCCTCTGCTG TTGCATCACC TGTGCTGGTC TCCATTAAAG 
ATGTTTACTG AGAATGGCAT GGAAACGGCT ATAGCCTGCT GGGAATGGCT TCTGGCTGGA 
AGAACAGGAG TTGAAGTCCC GTTCATGCGT GAGATGGCCG GCGCGTGGCA GATGACGGTG 
GATCTGAAGA TGGGGCTGTT TTCTGATGCC CAGCTGGAGG CCGATCCCCT GGCGGCTTCC 
GAGGAAAGCC AACCCATCCC CTGCCCGCCC GACGTCACGC CACACTACAT CTGGATTGAG 
TTTCTGGTGC AGCGGTTTGA GATCGCCAAG TACAGCAGCG CCGATCAGGT GGAGATTTTC 
GGCAGTCTTC TGCAGAGGTC CCTCTCTCTG AATGTCGGCA GCCCAAAAAG CAGTCTGAAC 
CGCCATGTGG CAGCCATCGG ACCTCGCTTC AGGTTGTTGA CGCTCGGACT TTCTCTCCTG 
CATTCTGACG TAGTGACCAA TGCCACCATC CGTAACGTCC TAAGGGAAAA GATTTATTCC 
ACTGCCTTCG ATTACTTCAG TGTGGCATCA AAGTTCCCCA CTCAGGGAGA CAAGCGTCTG 
CGGGAGGACA TCAGCATCAT GATCCGCTTC TACGCCAGCA TCCTCTCCGA CAAGAAGTAC 
CTAGGTGCTA GCCTGCTCGT GCCACCTGGT GAGCTCGGTG CCAATCCCCC TCAAAGCTCC 
CTATCTGCCG GGATTTTCGG ATCGCTTATC GTTTCCAGCA CTTCTCCGAT ATCGATCTTA 
AACAACCAGG ACCCGTCGCT GAACAGTCTA TCAGTGATGA ACGCCACCGA CCCCAGGAAC 
AACATGGACA TGACGGTGGG CTCCCGCCAG CAGAGCGCTC AGGGCTGGAT CAACACGTAC 
CCCCTGTCTA GCGGCATGTC CACCACCTCT AAGAAATCAG GCATGTCCAA AAAAAGCAAC 
AGAGGAACCC AGCTGCACAA GTACTACATG AAACGCAGGA CGTTGCTCTT GGCCTTGCTG 
GCCAGCGAGA TCGAGAGACT GAGCACATGG TACAACCCGC TGTCCACACA GGAGCTGGCC 
ATTCCCACCG AGCAGTCTGT GGAAACCAGC ATCTCCAACT GGAGGTCCAA ATACATCAAC 
CTGACCGAGA AACAGTGGAA GGATAATGTC AATTTGGCCT GGAGTATCGC TCCTTACCTG 
GCCTTGCAGC TTCCGGCAAG GTTCAAAAAC GCAGACGCCA TTGTGGCAGA AGTGACTCGT 
CTAGTTCGGC TGGATCCTGG TGCTGTCAGC GATGTGCCAG AGGCAGTGAA GTTCCTGGTA 
ACGTGGCACA CCATTGATGC AGATTCTCCA GAGCTGAGCC ACATCTTGTG CTGGGCTCCT 
GCAGATCCTC CGACCGGCCT CTCGTATTTC TCCAGCATGT ATCCTCCTCA TCCGCTCACT 
GCTCAGTATG GAGTCAAAGT CCTTCGCTCT TTTCCTCCTG ATGCCATTTT GTTTTACATT 
CCTCAAATCG TTCAGGCGCT GCGCTACGAC AAGATGGGTT ACGTGCGCGA GTACATCCTG 
TGGGCCGCAC AGAAGTCGCA GCTCCTCGCA CATCAGTTCA TATGGAACAT GAAGACCAAC 
ATCTTTCTGG ATGAAGAGGG ACATCAGAAA GACCCGGACA TCGGTGAGCT TCTGGAGCAG 
ATGGTGGAAG AGATCACGCA CTCTCTGTCA GGTCCAGCTA AAGACTTCTA TCAGCGCGAG 
TTTGACTTCT TCAACAAGAT CACCAACGTC TCCGCCATCA TCAAGCCTGT TCCCAAAGGA 
GATGAGAGGA AGAGAGCGTG TCTGAAGGCG CTGTCAGAAA TCAAAGTTCA GCCAGGATGT 
TATTTGCCAA GCAATCCAGA AGCAATCGTC CTTGATATCG ACTACAAGTC AGGAACGCCC 
ATGCAGAGCG CTGCCAAAGC CCCTTATCTG GCCAAATTCA AAGTTAAGAG GTGTGGCGTT 
AGTGAGCTTG AAAAAGAGGG TCTGCGCTGT CCGTCCGACT CTCAGGATGA AGGAGATGAG 
AGCTCACTGA CGGCTCAAAG GGTCTGCTGG CAGGCGGCCA TCTTTAAAGT GGGCGATGAT 
TGCAGACAGG ACATGCTCGC GCTTCAGATC ATTGGCCTGT TCAAGAACAT CTTTCAGCTG 
GTGGGTTTGG ATCTGTTTGT GTTCCCGTAC CGGGTTGTGG CCACAGCTCC AGGATGTGGC 
GTGATTGAAT GTATTCCGGA CTGTAAATCC CGGGATCAGC TCGGCCGGCA GACTGACTTC 
GGCATGTACG ACTACTTCAG GAACCAATAT GGCGACGAAT CCACTCTGGC TTTCCAGAAG 
GCTCGGTATA ACTTCATTCG CAGTATGGCT GCTTACAGTC TGCTGCTCTT TCTGTTACAA 
ATCAAAGACC GACACAACGG CAACATCATG CTGGACAGCA AAGGCCATCT CATTCACATC 
GACTTTGGCT TCATGTTTGA GAGTTCTCCT GGTGGGAATC TGGGCTGGGA GCCTGACATC 
AAGCTGACCG ATGAGATGGT GATGATCATG GGAGGCAAGA TGGAGGCTAC ACCATTTAAA 
TGGTTCATGG AGATGTGTGT GCGCGGATAC CTGGCTGTAA GGCCCTACAT GGATGCTGTC 
GTGTCATTGG TGACGTTGAT GCTGGACACG GGGTTGCCTT GCTTCAGAGG ACAGACGATC 
AAACTCCTCA AGCAACGGTT CAACCCAAAC ATGAGCGAGA AGGAAGCTGC GGCCTTCATG 
ATCAAAGTCA TCGAGCGCTG CTTCCTCAGT AGCAGGAGCA AAACCTACGA CATGCTGCAA 
TACTACCAGA ACCAAATCCC ATACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A Forward Genetic Screen Targeting the Endothelium Reveals a Regulatory Role for the Lipid Kinase Pi4ka in Myelo- and Erythropoiesis.
Cell reports22(5)1211-1224(2018 01)
Ziyad S,Riordan JD,Cavanaugh AM,Su T,Hernandez GE,Hilfenhaus G,Morselli M,Huynh K,Wang K,Chen JN,Dupuy AJ,Iruela-Arispe ML

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 03)
Bayés À,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Genetic Drivers of Kidney Defects in the DiGeorge Syndrome.
The New England journal of medicine376(8)742-754(2017 02)
Lopez-Rivera E,Liu YP,Verbitsky M,Anderson BR,Capone VP,Otto EA,Yan Z,Mitrotti A,Martino J,Steers NJ,Fasel DA,Vukojevic K,Deng R,Racedo SE,Liu Q,Werth M,Westland R,Vivante A,Makar GS,Bodria M,Sampson MG,Gillies CE,Vega-Warner V,Maiorana M,Petrey DS,Honig B,Lozanovski VJ,Salomon R,Heidet L,Carpentier W,Gaillard D,Carrea A,Gesualdo L,Cusi D,Izzi C,Scolari F,van Wijk JA,Arapovic A,Saraga-Babic M,Saraga M,Kunac N,Samii A,McDonald-McGinn DM,Crowley TB,Zackai EH,Drozdz D,Miklaszewska M,Tkaczyk M,Sikora P,Szczepanska M,Mizerska-Wasiak M,Krzemien G,Szmigielska A,Zaniew M,Darlow JM,Puri P,Barton D,Casolari E,Furth SL,Warady BA,Gucev Z,Hakonarson H,Flogelova H,Tasic V,Latos-Bielenska A,Materna-Kiryluk A,Allegri L,Wong CS,Drummond IA,D'Agati V,Imamoto A,Barasch JM,Hildebrandt F,Kiryluk K,Lifton RP,Morrow BE,Jeanpierre C,Papaioannou VE,Ghiggeri GM,Gharavi AG,Katsanis N,Sanna-Cherchi S

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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pi4kaa cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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