AFF4 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	AFF4
Entrez Gene ID	556421
Full Name	AF4/FMR2 family, member 4
General protein information	Preferred Names AF4/FMR2 family, member 4 Names AF4/FMR2 family member 4
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005173899.3	XP_005173956.1	AF4/FMR2 family member 4 isoform X1
XM_679235.10	XP_684327.6	AF4/FMR2 family member 4 isoform X3
XM_005173900.4	XP_005173957.1	AF4/FMR2 family member 4 isoform X2
XM_679235.9	XP_684327.6	AF4/FMR2 family member 4 isoform X3
XM_005173899.4	XP_005173956.1	AF4/FMR2 family member 4 isoform X1
XM_005173900.3	XP_005173957.1	AF4/FMR2 family member 4 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	AFF4	XP_517928.2
Canis lupus familiaris (dog)	AFF4	XP_850309.1
Mus musculus (house mouse)	Aff4	NP_291043.1
Gallus gallus (chicken)	AFF4	XP_004945088.1
Danio rerio (zebrafish)	aff4	XP_684327.6
Rattus norvegicus (Norway rat)	Aff4	NP_001100471.1
Xenopus tropicalis (tropical clawed frog)	aff4	XP_002935317.2
Homo sapiens (human)	AFF4	NP_055238.1
Bos taurus (cattle)	AFF4	NP_001178058.1

Gene Ontology

The following AFF4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AFF4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa26232	XM_005173899.4 Latest version!	Danio rerio AF4/FMR2 family, member 4 (aff4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa26233	XM_005173900.4 Latest version!	Danio rerio AF4/FMR2 family, member 4 (aff4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa26234	XM_679235.10 Latest version!	Danio rerio AF4/FMR2 family, member 4 (aff4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
ODa26234	XM_679235.9	Danio rerio AF4/FMR2 family, member 4 (aff4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa26232	XM_005173899.3	Danio rerio AF4/FMR2 family, member 4 (aff4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa26233	XM_005173900.3	Danio rerio AF4/FMR2 family, member 4 (aff4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AFF4 ( XM_005173899.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005173956.1 Danio rerio AF4/FMR2 family, member 4 (aff4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa26232
Clone ID Related Accession (Same CDS sequence)	XM_005173899.3 , XM_005173899.4
Accession Version	XM_005173899.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	AF4/FMR2 family member 4 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173899.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCTC AGTCAGGCAA CATGAACCGT GAAGACCGGA ATGTGCTCCG TATGAAAGAA 
AGGGAAAGGA GAAATCAAGA AATCCAGCAG GGAGGAGAGG CCTTTCCAGC TAACTCCCCC 
CTCTTTCCTG AACCTATTAA AGTTGCCAAG GAAGATAAAC TCTCAAGTCG CATCCAGAGC 
ATGCTGGGAA ATTACGACGA GATGAAGGAA ACCATTGGTG AACCGCCAAT GTCCAAGCTC 
ATTTCCAAAG CTTCCAGCTC TTCCTCCGAG GATAAATCTG GCCAGTATGG TGACCAGCGC 
GGAGGCTCTC AGACTCAAAG CAGCAAGTGG ACCCCGGTGG GTCCGGCCTC AGGAGGTGGC 
AACTCTTCAT CGTCTTCTCA GAAACGCTCA ACTGTGCAGA GCGTCAGCCA GAAGAGCAGC 
TCCAGCGGGC AGCGGCACGA GCGGGACTAC AGCGGCAGCA AGAAATCCAG CAAGCACAGC 
AACGAGCACA AGTCCCACTC CAGTTCCAGC CCGGCCAAAG TCTCCAGCAA CCACTCGCGC 
CGAGTCCCCA AGTCCCCCAG AGACAGAGAG GCCAATTGGG ACTCGCCCTC CATCCCGCAT 
TCCTTCCCCA GTGGACATCC CAACCAAACC TTCCCACCCT CGCTCATCAA ATCCAGCTCC 
ATGCCCCAGA AGCCCACCGC TTACGTTCGG CCCATGGACG GCCAGGAGAC GACCGAGGCA 
AAGACGTCTT CAGATTCGTA CAGCGGCCAA TCGCACAGCA GCACAGTTGG AGAAATGAAG 
CCCAATGGAA AGGGCCCACT CCCCAAGCTC AAGATCCCAA CACAGCCTCC AGAGGCGGGG 
GATGCGAGCT GTGTTGATGA GATTTTAAAG GAAATGACTC AGGTCTGGCC TCCTCCATTA 
ACAGCCATTC ACACCCCCTG CAAGACCGAG CCGCCTTCCA AATTCCCCTT CTCCAACGCT 
AAGGATGCCC AGCACCAGTC TGTTGGGGCT CAAAAAAAGT TCTCAGGGAA GAGCGCATCA 
ACCAGTCACC CGTCAAAGCC ATGTGACAGC GAGCAGTCCA ACATGCTTCA TGATGATCTT 
AAGCTCAGCA GCAGTGAAGA CAGTGACGGA GAGCAAGATC CTGCCAAAAA CACCCCTCAA 
AACAACTCCA CAAGTAACAA CAGTAATAAC AGTGAGGGAG CGGAGCGAGA GGACACCAGC 
AGTCACAGCG GCTCCGAGAG CAGCTCCGGG TCCGACAGCG AGAGCGAGAG CAGCTCCACC 
GACAGCGAGA CCACCGAACC GCCACGAGCT GCGACCCCCG AGCGGGAACA GCCTACGGCT 
AACAAATGGC TTCTGGACAA CTGGTTAAAT AAGGTCAACA AGCTGTCTCC TGCTTCTCCG 
GTGGAAAACA GCAGCAGCAG CACGCCGGGA ACCAAGTATA AGAAGGAGGG TCGAGATACC 
AGCTCCAGCA GGAGTTACAG TGGGCAGGGG TCTAAAGAAG CTGCAACGTC AGCCGGCCGA 
GAGTTGCGAC CCAATCAGAA GAGCTCCGAA AGCTGGCGAG GACGCAGTCA GAAATCTCCT 
GCTCAGAGCG AGGGCACAAG TCAATCCGGG CGACGGGTCG TAGGCAAAAA ACAGCCCAAA 
AAACCTGAGA AGCCGCCGGT GGTTGAAGAG CCCAAGGGTG GACTAAAAGT AGAAACCGAA 
CCATCGCCGG AAATCCCGTC ACACAGAGCT CGCGCTCCCA ATAAAGGCCT GCGCAAACCC 
AACATTAAAA AAGAACCCAA AACCTCCTCT CCACGACCTC CGCCTGATAA GCGCAAGACG 
AAAGCGGCAA AAGTGCCCAG CAAGTCTCGC GAGTTCATCG AATCGGATTC GTCGACGTCG 
GATTCTGAAG GCAACGAAAG CGACCCATTG CCTTCTCAGA CGCCGAAAGA TTACAGCGAA 
AACCCGTTGG TGCCACTCTC GCCCATTTAT GACCCGCCCA GACTGCTGGT GCAGATCAAT 
CTCAGCCTGC TGACTAGAGT CCCGGGACAG CTCTACAATG AGACGGAGGT TAAGGTGGAG 
CGAGGGTCTC CAGCGGAGGG AAAAAGCAAC CAGAAGCAGC CCGGAGAAAA GATAAGCAAG 
GAAGGAAGGA AGAGGAAACA CAAGAATGAA GATGATGGTG TGAAACCAGA GAGTAAGAAG 
TCCAAACTGG ATGAGAAGTC TTCACGCAAA TCAAGCAGTA ATGATTCTTC CAAGCGTGCT 
CAGGAAAAAG AGAAGGAGTC TGCGCCGTCT CCCTCCATCA CACTGCAGCG GACGCCCAAA 
TCTGAGCAGC AGGGGCGCAA GCGGACCCTC AGCCAGTCCT CCAGCACCGT CCCCAGCAAA 
GACAAGAGCA GCTCCAGCTC CAGACACCGC AGAGGAGACG ACAAACCCCA GCGCAGAGAG 
CCCAAGGAGA AAAGCTCCAA ATCAGAGAAT CCCGTTTCAG TGCCGCCGCT CTCTGAGGGC 
TCCAAATCTA GATCTAAACT TCTGTTTGAG GACAGGGTTC ATTCGGCTGA TCATTACCTT 
CAAGAAGCCA AGAAGTTGAA ACACAATGCG GATGCTCTGG TGAGGATGGA CAGATTTGAG 
AAGGCGGTGT ATTACCTGGA CGCCGTGGTG TCTTTCATCG AGTGTGGAAA TGCCCTGGAG 
AAAAACGCCC AGGAGGCCAA ATCCCCCTTT CCCATGTATG CAGAAACCGT GGAGCTCATC 
AAGTACACTA TGAAGCTAAA GAGCTACATG GCACCAGATG CAACTTCTCA AGACAAAAGG 
CTAGCAGTGC TATGTCTTCG ATGTCAAGCG CTCCTGTATT TGAGGCTTTT CAAGCTGCGG 
AAAGAAAGTG CACTGAAATA CTCTAAAACA CTCACTGAAC ACTTGAAGAA TTCATTGAGT 
AATACCCAAG CTCCGTCTCC TGGAGTAGCA AATAAAAGCG CAGGGATGCC GTCTCCGGTC 
TCCCCCAAAC TCTCTCCTGG CAGTGCGGGC AGCTACTCCT CCAGCAGCTC CAGCCAAAGC 
GCCAGCTCCT CCGTCACCAT CCCGCAGCGC ATCCACCAGA TGGCGGCAAG CTACGTGCAA 
GTCACCTCCA ACTTCCTGTA CGCCACCGAG GTGTGGGACC AGGCTGAGCA GCTCGCCAAA 
GAGCAGAGAG AGTTCTTCAC TGAGCTGGAC AAGGCGATGG GTCCGCTGAT CTTCAACACC 
AGCAGCATGA CTGACCTGGT GCGGTTCACG CGGCAGGGCC TGCACTGGCT ACGACTGGAC 
GCCAAGCTCA TCCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005173956.1
CDS	528..3785
Translation	MASQSGNMNREDRNVLRMKERERRNQEIQQGGEAFPANSPLFPEPIKVAKEDKLSSRIQSMLGNYDEMKETIGEPPMSKLISKASSSSSEDKSGQYGDQRGGSQTQSSKWTPVGPASGGGNSSSSSQKRSTVQSVSQKSSSSGQRHERDYSGSKKSSKHSNEHKSHSSSSPAKVSSNHSRRVPKSPRDREANWDSPSIPHSFPSGHPNQTFPPSLIKSSSMPQKPTAYVRPMDGQETTEAKTSSDSYSGQSHSSTVGEMKPNGKGPLPKLKIPTQPPEAGDASCVDEILKEMTQVWPPPLTAIHTPCKTEPPSKFPFSNAKDAQHQSVGAQKKFSGKSASTSHPSKPCDSEQSNMLHDDLKLSSSEDSDGEQDPAKNTPQNNSTSNNSNNSEGAEREDTSSHSGSESSSGSDSESESSSTDSETTEPPRAATPEREQPTANKWLLDNWLNKVNKLSPASPVENSSSSTPGTKYKKEGRDTSSSRSYSGQGSKEAATSAGRELRPNQKSSESWRGRSQKSPAQSEGTSQSGRRVVGKKQPKKPEKPPVVEEPKGGLKVETEPSPEIPSHRARAPNKGLRKPNIKKEPKTSSPRPPPDKRKTKAAKVPSKSREFIESDSSTSDSEGNESDPLPSQTPKDYSENPLVPLSPIYDPPRLLVQINLSLLTRVPGQLYNETEVKVERGSPAEGKSNQKQPGEKISKEGRKRKHKNEDDGVKPESKKSKLDEKSSRKSSSNDSSKRAQEKEKESAPSPSITLQRTPKSEQQGRKRTLSQSSSTVPSKDKSSSSSRHRRGDDKPQRREPKEKSSKSENPVSVPPLSEGSKSRSKLLFEDRVHSADHYLQEAKKLKHNADALVRMDRFEKAVYYLDAVVSFIECGNALEKNAQEAKSPFPMYAETVELIKYTMKLKSYMAPDATSQDKRLAVLCLRCQALLYLRLFKLRKESALKYSKTLTEHLKNSLSNTQAPSPGVANKSAGMPSPVSPKLSPGSAGSYSSSSSSQSASSSVTIPQRIHQMAASYVQVTSNFLYATEVWDQAEQLAKEQREFFTELDKAMGPLIFNTSSMTDLVRFTRQGLHWLRLDAKLIQ

Target ORF information:

RefSeq Version	XM_005173899.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio AF4/FMR2 family, member 4 (aff4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173899.3

ORF Insert Sequence:

ATGGCCTCTC AGTCAGGCAA CATGAACCGT GAAGACCGGA ATGTGCTCCG TATGAAAGAA 
AGGGAAAGGA GAAATCAAGA AATCCAGCAG GGAGGAGAGG CCTTTCCAGC TAACTCCCCC 
CTCTTTCCTG AACCTATTAA AGTTGCCAAG GAAGATAAAC TCTCAAGTCG CATCCAGAGC 
ATGCTGGGAA ATTACGACGA GATGAAGGAA ACCATTGGTG AACCGCCAAT GTCCAAGCTC 
ATTTCCAAAG CTTCCAGCTC TTCCTCCGAG GATAAATCTG GCCAGTATGG TGACCAGCGC 
GGAGGCTCTC AGACTCAAAG CAGCAAGTGG ACCCCGGTGG GTCCGGCCTC AGGAGGTGGC 
AACTCTTCAT CGTCTTCTCA GAAACGCTCA ACTGTGCAGA GCGTCAGCCA GAAGAGCAGC 
TCCAGCGGGC AGCGGCACGA GCGGGACTAC AGCGGCAGCA AGAAATCCAG CAAGCACAGC 
AACGAGCACA AGTCCCACTC CAGTTCCAGC CCGGCCAAAG TCTCCAGCAA CCACTCGCGC 
CGAGTCCCCA AGTCCCCCAG AGACAGAGAG GCCAATTGGG ACTCGCCCTC CATCCCGCAT 
TCCTTCCCCA GTGGACATCC CAACCAAACC TTCCCACCCT CGCTCATCAA ATCCAGCTCC 
ATGCCCCAGA AGCCCACCGC TTACGTTCGG CCCATGGACG GCCAGGAGAC GACCGAGGCA 
AAGACGTCTT CAGATTCGTA CAGCGGCCAA TCGCACAGCA GCACAGTTGG AGAAATGAAG 
CCCAATGGAA AGGGCCCACT CCCCAAGCTC AAGATCCCAA CACAGCCTCC AGAGGCGGGG 
GATGCGAGCT GTGTTGATGA GATTTTAAAG GAAATGACTC AGGTCTGGCC TCCTCCATTA 
ACAGCCATTC ACACCCCCTG CAAGACCGAG CCGCCTTCCA AATTCCCCTT CTCCAACGCT 
AAGGATGCCC AGCACCAGTC TGTTGGGGCT CAAAAAAAGT TCTCAGGGAA GAGCGCATCA 
ACCAGTCACC CGTCAAAGCC ATGTGACAGC GAGCAGTCCA ACATGCTTCA TGATGATCTT 
AAGCTCAGCA GCAGTGAAGA CAGTGACGGA GAGCAAGATC CTGCCAAAAA CACCCCTCAA 
AACAACTCCA CAAGTAACAA CAGTAATAAC AGTGAGGGAG CGGAGCGAGA GGACACCAGC 
AGTCACAGCG GCTCCGAGAG CAGCTCCGGG TCCGACAGCG AGAGCGAGAG CAGCTCCACC 
GACAGCGAGA CCACCGAACC GCCACGAGCT GCGACCCCCG AGCGGGAACA GCCTACGGCT 
AACAAATGGC TTCTGGACAA CTGGTTAAAT AAGGTCAACA AGCTGTCTCC TGCTTCTCCG 
GTGGAAAACA GCAGCAGCAG CACGCCGGGA ACCAAGTATA AGAAGGAGGG TCGAGATACC 
AGCTCCAGCA GGAGTTACAG TGGGCAGGGG TCTAAAGAAG CTGCAACGTC AGCCGGCCGA 
GAGTTGCGAC CCAATCAGAA GAGCTCCGAA AGCTGGCGAG GACGCAGTCA GAAATCTCCT 
GCTCAGAGCG AGGGCACAAG TCAATCCGGG CGACGGGTCG TAGGCAAAAA ACAGCCCAAA 
AAACCTGAGA AGCCGCCGGT GGTTGAAGAG CCCAAGGGTG GACTAAAAGT AGAAACCGAA 
CCATCGCCGG AAATCCCGTC ACACAGAGCT CGCGCTCCCA ATAAAGGCCT GCGCAAACCC 
AACATTAAAA AAGAACCCAA AACCTCCTCT CCACGACCTC CGCCTGATAA GCGCAAGACG 
AAAGCGGCAA AAGTGCCCAG CAAGTCTCGC GAGTTCATCG AATCGGATTC GTCGACGTCG 
GATTCTGAAG GCAACGAAAG CGACCCATTG CCTTCTCAGA CGCCGAAAGA TTACAGCGAA 
AACCCGTTGG TGCCACTCTC GCCCATTTAT GACCCGCCCA GACTGCTGGT GCAGATCAAT 
CTCAGCCTGC TGACTAGAGT CCCGGGACAG CTCTACAATG AGACGGAGGT TAAGGTGGAG 
CGAGGGTCTC CAGCGGAGGG AAAAAGCAAC CAGAAGCAGC CCGGAGAAAA GATAAGCAAG 
GAAGGAAGGA AGAGGAAACA CAAGAATGAA GATGATGGTG TGAAACCAGA GAGTAAGAAG 
TCCAAACTGG ATGAGAAGTC TTCACGCAAA TCAAGCAGTA ATGATTCTTC CAAGCGTGCT 
CAGGAAAAAG AGAAGGAGTC TGCGCCGTCT CCCTCCATCA CACTGCAGCG GACGCCCAAA 
TCTGAGCAGC AGGGGCGCAA GCGGACCCTC AGCCAGTCCT CCAGCACCGT CCCCAGCAAA 
GACAAGAGCA GCTCCAGCTC CAGACACCGC AGAGGAGACG ACAAACCCCA GCGCAGAGAG 
CCCAAGGAGA AAAGCTCCAA ATCAGAGAAT CCCGTTTCAG TGCCGCCGCT CTCTGAGGGC 
TCCAAATCTA GATCTAAACT TCTGTTTGAG GACAGGGTTC ATTCGGCTGA TCATTACCTT 
CAAGAAGCCA AGAAGTTGAA ACACAATGCG GATGCTCTGG TGAGGATGGA CAGATTTGAG 
AAGGCGGTGT ATTACCTGGA CGCCGTGGTG TCTTTCATCG AGTGTGGAAA TGCCCTGGAG 
AAAAACGCCC AGGAGGCCAA ATCCCCCTTT CCCATGTATG CAGAAACCGT GGAGCTCATC 
AAGTACACTA TGAAGCTAAA GAGCTACATG GCACCAGATG CAACTTCTCA AGACAAAAGG 
CTAGCAGTGC TATGTCTTCG ATGTCAAGCG CTCCTGTATT TGAGGCTTTT CAAGCTGCGG 
AAAGAAAGTG CACTGAAATA CTCTAAAACA CTCACTGAAC ACTTGAAGAA TTCATTGAGT 
AATACCCAAG CTCCGTCTCC TGGAGTAGCA AATAAAAGCG CAGGGATGCC GTCTCCGGTC 
TCCCCCAAAC TCTCTCCTGG CAGTGCGGGC AGCTACTCCT CCAGCAGCTC CAGCCAAAGC 
GCCAGCTCCT CCGTCACCAT CCCGCAGCGC ATCCACCAGA TGGCGGCAAG CTACGTGCAA 
GTCACCTCCA ACTTCCTGTA CGCCACCGAG GTGTGGGACC AGGCTGAGCA GCTCGCCAAA 
GAGCAGAGAG AGTTCTTCAC TGAGCTGGAC AAGGCGATGG GTCCGCTGAT CTTCAACACC 
AGCAGCATGA CTGACCTGGT GCGGTTCACG CGGCAGGGCC TGCACTGGCT ACGACTGGAC 
GCCAAGCTCA TCCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF4 ( XM_005173899.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005173956.1 Danio rerio AF4/FMR2 family, member 4 (aff4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa26232
Clone ID Related Accession (Same CDS sequence)	XM_005173899.3 , XM_005173899.4
Accession Version	XM_005173899.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	AF4/FMR2 family member 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005173899.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCTC AGTCAGGCAA CATGAACCGT GAAGACCGGA ATGTGCTCCG TATGAAAGAA 
AGGGAAAGGA GAAATCAAGA AATCCAGCAG GGAGGAGAGG CCTTTCCAGC TAACTCCCCC 
CTCTTTCCTG AACCTATTAA AGTTGCCAAG GAAGATAAAC TCTCAAGTCG CATCCAGAGC 
ATGCTGGGAA ATTACGACGA GATGAAGGAA ACCATTGGTG AACCGCCAAT GTCCAAGCTC 
ATTTCCAAAG CTTCCAGCTC TTCCTCCGAG GATAAATCTG GCCAGTATGG TGACCAGCGC 
GGAGGCTCTC AGACTCAAAG CAGCAAGTGG ACCCCGGTGG GTCCGGCCTC AGGAGGTGGC 
AACTCTTCAT CGTCTTCTCA GAAACGCTCA ACTGTGCAGA GCGTCAGCCA GAAGAGCAGC 
TCCAGCGGGC AGCGGCACGA GCGGGACTAC AGCGGCAGCA AGAAATCCAG CAAGCACAGC 
AACGAGCACA AGTCCCACTC CAGTTCCAGC CCGGCCAAAG TCTCCAGCAA CCACTCGCGC 
CGAGTCCCCA AGTCCCCCAG AGACAGAGAG GCCAATTGGG ACTCGCCCTC CATCCCGCAT 
TCCTTCCCCA GTGGACATCC CAACCAAACC TTCCCACCCT CGCTCATCAA ATCCAGCTCC 
ATGCCCCAGA AGCCCACCGC TTACGTTCGG CCCATGGACG GCCAGGAGAC GACCGAGGCA 
AAGACGTCTT CAGATTCGTA CAGCGGCCAA TCGCACAGCA GCACAGTTGG AGAAATGAAG 
CCCAATGGAA AGGGCCCACT CCCCAAGCTC AAGATCCCAA CACAGCCTCC AGAGGCGGGG 
GATGCGAGCT GTGTTGATGA GATTTTAAAG GAAATGACTC AGGTCTGGCC TCCTCCATTA 
ACAGCCATTC ACACCCCCTG CAAGACCGAG CCGCCTTCCA AATTCCCCTT CTCCAACGCT 
AAGGATGCCC AGCACCAGTC TGTTGGGGCT CAAAAAAAGT TCTCAGGGAA GAGCGCATCA 
ACCAGTCACC CGTCAAAGCC ATGTGACAGC GAGCAGTCCA ACATGCTTCA TGATGATCTT 
AAGCTCAGCA GCAGTGAAGA CAGTGACGGA GAGCAAGATC CTGCCAAAAA CACCCCTCAA 
AACAACTCCA CAAGTAACAA CAGTAATAAC AGTGAGGGAG CGGAGCGAGA GGACACCAGC 
AGTCACAGCG GCTCCGAGAG CAGCTCCGGG TCCGACAGCG AGAGCGAGAG CAGCTCCACC 
GACAGCGAGA CCACCGAACC GCCACGAGCT GCGACCCCCG AGCGGGAACA GCCTACGGCT 
AACAAATGGC TTCTGGACAA CTGGTTAAAT AAGGTCAACA AGCTGTCTCC TGCTTCTCCG 
GTGGAAAACA GCAGCAGCAG CACGCCGGGA ACCAAGTATA AGAAGGAGGG TCGAGATACC 
AGCTCCAGCA GGAGTTACAG TGGGCAGGGG TCTAAAGAAG CTGCAACGTC AGCCGGCCGA 
GAGTTGCGAC CCAATCAGAA GAGCTCCGAA AGCTGGCGAG GACGCAGTCA GAAATCTCCT 
GCTCAGAGCG AGGGCACAAG TCAATCCGGG CGACGGGTCG TAGGCAAAAA ACAGCCCAAA 
AAACCTGAGA AGCCGCCGGT GGTTGAAGAG CCCAAGGGTG GACTAAAAGT AGAAACCGAA 
CCATCGCCGG AAATCCCGTC ACACAGAGCT CGCGCTCCCA ATAAAGGCCT GCGCAAACCC 
AACATTAAAA AAGAACCCAA AACCTCCTCT CCACGACCTC CGCCTGATAA GCGCAAGACG 
AAAGCGGCAA AAGTGCCCAG CAAGTCTCGC GAGTTCATCG AATCGGATTC GTCGACGTCG 
GATTCTGAAG GCAACGAAAG CGACCCATTG CCTTCTCAGA CGCCGAAAGA TTACAGCGAA 
AACCCGTTGG TGCCACTCTC GCCCATTTAT GACCCGCCCA GACTGCTGGT GCAGATCAAT 
CTCAGCCTGC TGACTAGAGT CCCGGGACAG CTCTACAATG AGACGGAGGT TAAGGTGGAG 
CGAGGGTCTC CAGCGGAGGG AAAAAGCAAC CAGAAGCAGC CCGGAGAAAA GATAAGCAAG 
GAAGGAAGGA AGAGGAAACA CAAGAATGAA GATGATGGTG TGAAACCAGA GAGTAAGAAG 
TCCAAACTGG ATGAGAAGTC TTCACGCAAA TCAAGCAGTA ATGATTCTTC CAAGCGTGCT 
CAGGAAAAAG AGAAGGAGTC TGCGCCGTCT CCCTCCATCA CACTGCAGCG GACGCCCAAA 
TCTGAGCAGC AGGGGCGCAA GCGGACCCTC AGCCAGTCCT CCAGCACCGT CCCCAGCAAA 
GACAAGAGCA GCTCCAGCTC CAGACACCGC AGAGGAGACG ACAAACCCCA GCGCAGAGAG 
CCCAAGGAGA AAAGCTCCAA ATCAGAGAAT CCCGTTTCAG TGCCGCCGCT CTCTGAGGGC 
TCCAAATCTA GATCTAAACT TCTGTTTGAG GACAGGGTTC ATTCGGCTGA TCATTACCTT 
CAAGAAGCCA AGAAGTTGAA ACACAATGCG GATGCTCTGG TGAGGATGGA CAGATTTGAG 
AAGGCGGTGT ATTACCTGGA CGCCGTGGTG TCTTTCATCG AGTGTGGAAA TGCCCTGGAG 
AAAAACGCCC AGGAGGCCAA ATCCCCCTTT CCCATGTATG CAGAAACCGT GGAGCTCATC 
AAGTACACTA TGAAGCTAAA GAGCTACATG GCACCAGATG CAACTTCTCA AGACAAAAGG 
CTAGCAGTGC TATGTCTTCG ATGTCAAGCG CTCCTGTATT TGAGGCTTTT CAAGCTGCGG 
AAAGAAAGTG CACTGAAATA CTCTAAAACA CTCACTGAAC ACTTGAAGAA TTCATTGAGT 
AATACCCAAG CTCCGTCTCC TGGAGTAGCA AATAAAAGCG CAGGGATGCC GTCTCCGGTC 
TCCCCCAAAC TCTCTCCTGG CAGTGCGGGC AGCTACTCCT CCAGCAGCTC CAGCCAAAGC 
GCCAGCTCCT CCGTCACCAT CCCGCAGCGC ATCCACCAGA TGGCGGCAAG CTACGTGCAA 
GTCACCTCCA ACTTCCTGTA CGCCACCGAG GTGTGGGACC AGGCTGAGCA GCTCGCCAAA 
GAGCAGAGAG AGTTCTTCAC TGAGCTGGAC AAGGCGATGG GTCCGCTGAT CTTCAACACC 
AGCAGCATGA CTGACCTGGT GCGGTTCACG CGGCAGGGCC TGCACTGGCT ACGACTGGAC 
GCCAAGCTCA TCCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005173956.1
CDS	476..3733
Translation	MASQSGNMNREDRNVLRMKERERRNQEIQQGGEAFPANSPLFPEPIKVAKEDKLSSRIQSMLGNYDEMKETIGEPPMSKLISKASSSSSEDKSGQYGDQRGGSQTQSSKWTPVGPASGGGNSSSSSQKRSTVQSVSQKSSSSGQRHERDYSGSKKSSKHSNEHKSHSSSSPAKVSSNHSRRVPKSPRDREANWDSPSIPHSFPSGHPNQTFPPSLIKSSSMPQKPTAYVRPMDGQETTEAKTSSDSYSGQSHSSTVGEMKPNGKGPLPKLKIPTQPPEAGDASCVDEILKEMTQVWPPPLTAIHTPCKTEPPSKFPFSNAKDAQHQSVGAQKKFSGKSASTSHPSKPCDSEQSNMLHDDLKLSSSEDSDGEQDPAKNTPQNNSTSNNSNNSEGAEREDTSSHSGSESSSGSDSESESSSTDSETTEPPRAATPEREQPTANKWLLDNWLNKVNKLSPASPVENSSSSTPGTKYKKEGRDTSSSRSYSGQGSKEAATSAGRELRPNQKSSESWRGRSQKSPAQSEGTSQSGRRVVGKKQPKKPEKPPVVEEPKGGLKVETEPSPEIPSHRARAPNKGLRKPNIKKEPKTSSPRPPPDKRKTKAAKVPSKSREFIESDSSTSDSEGNESDPLPSQTPKDYSENPLVPLSPIYDPPRLLVQINLSLLTRVPGQLYNETEVKVERGSPAEGKSNQKQPGEKISKEGRKRKHKNEDDGVKPESKKSKLDEKSSRKSSSNDSSKRAQEKEKESAPSPSITLQRTPKSEQQGRKRTLSQSSSTVPSKDKSSSSSRHRRGDDKPQRREPKEKSSKSENPVSVPPLSEGSKSRSKLLFEDRVHSADHYLQEAKKLKHNADALVRMDRFEKAVYYLDAVVSFIECGNALEKNAQEAKSPFPMYAETVELIKYTMKLKSYMAPDATSQDKRLAVLCLRCQALLYLRLFKLRKESALKYSKTLTEHLKNSLSNTQAPSPGVANKSAGMPSPVSPKLSPGSAGSYSSSSSSQSASSSVTIPQRIHQMAASYVQVTSNFLYATEVWDQAEQLAKEQREFFTELDKAMGPLIFNTSSMTDLVRFTRQGLHWLRLDAKLIQ

Target ORF information:

RefSeq Version	XM_005173899.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio AF4/FMR2 family, member 4 (aff4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173899.4

ORF Insert Sequence:

ATGGCCTCTC AGTCAGGCAA CATGAACCGT GAAGACCGGA ATGTGCTCCG TATGAAAGAA 
AGGGAAAGGA GAAATCAAGA AATCCAGCAG GGAGGAGAGG CCTTTCCAGC TAACTCCCCC 
CTCTTTCCTG AACCTATTAA AGTTGCCAAG GAAGATAAAC TCTCAAGTCG CATCCAGAGC 
ATGCTGGGAA ATTACGACGA GATGAAGGAA ACCATTGGTG AACCGCCAAT GTCCAAGCTC 
ATTTCCAAAG CTTCCAGCTC TTCCTCCGAG GATAAATCTG GCCAGTATGG TGACCAGCGC 
GGAGGCTCTC AGACTCAAAG CAGCAAGTGG ACCCCGGTGG GTCCGGCCTC AGGAGGTGGC 
AACTCTTCAT CGTCTTCTCA GAAACGCTCA ACTGTGCAGA GCGTCAGCCA GAAGAGCAGC 
TCCAGCGGGC AGCGGCACGA GCGGGACTAC AGCGGCAGCA AGAAATCCAG CAAGCACAGC 
AACGAGCACA AGTCCCACTC CAGTTCCAGC CCGGCCAAAG TCTCCAGCAA CCACTCGCGC 
CGAGTCCCCA AGTCCCCCAG AGACAGAGAG GCCAATTGGG ACTCGCCCTC CATCCCGCAT 
TCCTTCCCCA GTGGACATCC CAACCAAACC TTCCCACCCT CGCTCATCAA ATCCAGCTCC 
ATGCCCCAGA AGCCCACCGC TTACGTTCGG CCCATGGACG GCCAGGAGAC GACCGAGGCA 
AAGACGTCTT CAGATTCGTA CAGCGGCCAA TCGCACAGCA GCACAGTTGG AGAAATGAAG 
CCCAATGGAA AGGGCCCACT CCCCAAGCTC AAGATCCCAA CACAGCCTCC AGAGGCGGGG 
GATGCGAGCT GTGTTGATGA GATTTTAAAG GAAATGACTC AGGTCTGGCC TCCTCCATTA 
ACAGCCATTC ACACCCCCTG CAAGACCGAG CCGCCTTCCA AATTCCCCTT CTCCAACGCT 
AAGGATGCCC AGCACCAGTC TGTTGGGGCT CAAAAAAAGT TCTCAGGGAA GAGCGCATCA 
ACCAGTCACC CGTCAAAGCC ATGTGACAGC GAGCAGTCCA ACATGCTTCA TGATGATCTT 
AAGCTCAGCA GCAGTGAAGA CAGTGACGGA GAGCAAGATC CTGCCAAAAA CACCCCTCAA 
AACAACTCCA CAAGTAACAA CAGTAATAAC AGTGAGGGAG CGGAGCGAGA GGACACCAGC 
AGTCACAGCG GCTCCGAGAG CAGCTCCGGG TCCGACAGCG AGAGCGAGAG CAGCTCCACC 
GACAGCGAGA CCACCGAACC GCCACGAGCT GCGACCCCCG AGCGGGAACA GCCTACGGCT 
AACAAATGGC TTCTGGACAA CTGGTTAAAT AAGGTCAACA AGCTGTCTCC TGCTTCTCCG 
GTGGAAAACA GCAGCAGCAG CACGCCGGGA ACCAAGTATA AGAAGGAGGG TCGAGATACC 
AGCTCCAGCA GGAGTTACAG TGGGCAGGGG TCTAAAGAAG CTGCAACGTC AGCCGGCCGA 
GAGTTGCGAC CCAATCAGAA GAGCTCCGAA AGCTGGCGAG GACGCAGTCA GAAATCTCCT 
GCTCAGAGCG AGGGCACAAG TCAATCCGGG CGACGGGTCG TAGGCAAAAA ACAGCCCAAA 
AAACCTGAGA AGCCGCCGGT GGTTGAAGAG CCCAAGGGTG GACTAAAAGT AGAAACCGAA 
CCATCGCCGG AAATCCCGTC ACACAGAGCT CGCGCTCCCA ATAAAGGCCT GCGCAAACCC 
AACATTAAAA AAGAACCCAA AACCTCCTCT CCACGACCTC CGCCTGATAA GCGCAAGACG 
AAAGCGGCAA AAGTGCCCAG CAAGTCTCGC GAGTTCATCG AATCGGATTC GTCGACGTCG 
GATTCTGAAG GCAACGAAAG CGACCCATTG CCTTCTCAGA CGCCGAAAGA TTACAGCGAA 
AACCCGTTGG TGCCACTCTC GCCCATTTAT GACCCGCCCA GACTGCTGGT GCAGATCAAT 
CTCAGCCTGC TGACTAGAGT CCCGGGACAG CTCTACAATG AGACGGAGGT TAAGGTGGAG 
CGAGGGTCTC CAGCGGAGGG AAAAAGCAAC CAGAAGCAGC CCGGAGAAAA GATAAGCAAG 
GAAGGAAGGA AGAGGAAACA CAAGAATGAA GATGATGGTG TGAAACCAGA GAGTAAGAAG 
TCCAAACTGG ATGAGAAGTC TTCACGCAAA TCAAGCAGTA ATGATTCTTC CAAGCGTGCT 
CAGGAAAAAG AGAAGGAGTC TGCGCCGTCT CCCTCCATCA CACTGCAGCG GACGCCCAAA 
TCTGAGCAGC AGGGGCGCAA GCGGACCCTC AGCCAGTCCT CCAGCACCGT CCCCAGCAAA 
GACAAGAGCA GCTCCAGCTC CAGACACCGC AGAGGAGACG ACAAACCCCA GCGCAGAGAG 
CCCAAGGAGA AAAGCTCCAA ATCAGAGAAT CCCGTTTCAG TGCCGCCGCT CTCTGAGGGC 
TCCAAATCTA GATCTAAACT TCTGTTTGAG GACAGGGTTC ATTCGGCTGA TCATTACCTT 
CAAGAAGCCA AGAAGTTGAA ACACAATGCG GATGCTCTGG TGAGGATGGA CAGATTTGAG 
AAGGCGGTGT ATTACCTGGA CGCCGTGGTG TCTTTCATCG AGTGTGGAAA TGCCCTGGAG 
AAAAACGCCC AGGAGGCCAA ATCCCCCTTT CCCATGTATG CAGAAACCGT GGAGCTCATC 
AAGTACACTA TGAAGCTAAA GAGCTACATG GCACCAGATG CAACTTCTCA AGACAAAAGG 
CTAGCAGTGC TATGTCTTCG ATGTCAAGCG CTCCTGTATT TGAGGCTTTT CAAGCTGCGG 
AAAGAAAGTG CACTGAAATA CTCTAAAACA CTCACTGAAC ACTTGAAGAA TTCATTGAGT 
AATACCCAAG CTCCGTCTCC TGGAGTAGCA AATAAAAGCG CAGGGATGCC GTCTCCGGTC 
TCCCCCAAAC TCTCTCCTGG CAGTGCGGGC AGCTACTCCT CCAGCAGCTC CAGCCAAAGC 
GCCAGCTCCT CCGTCACCAT CCCGCAGCGC ATCCACCAGA TGGCGGCAAG CTACGTGCAA 
GTCACCTCCA ACTTCCTGTA CGCCACCGAG GTGTGGGACC AGGCTGAGCA GCTCGCCAAA 
GAGCAGAGAG AGTTCTTCAC TGAGCTGGAC AAGGCGATGG GTCCGCTGAT CTTCAACACC 
AGCAGCATGA CTGACCTGGT GCGGTTCACG CGGCAGGGCC TGCACTGGCT ACGACTGGAC 
GCCAAGCTCA TCCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF4 ( XM_005173900.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005173957.1 Danio rerio AF4/FMR2 family, member 4 (aff4), transcript variant X2, mRNA.

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CloneID	ODa26233
Clone ID Related Accession (Same CDS sequence)	XM_005173900.4 , XM_005173900.3
Accession Version	XM_005173900.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	AF4/FMR2 family member 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005173900.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCTC AGTCAGGCAA CATGAACCGT GAAGACCGGA ATGTGCTCCG TATGAAAGAA 
AGGGAAAGGA GAAATCAAGA AATCCAGCAG GGAGGAGAGG CCTTTCCAGC TAACTCCCCC 
CTCTTTCCTG AACCTATTAA AGTTGCCAAG GAAGATAAAC TCTCAAGTCG CATCCAGAGC 
ATGCTGGGAA ATTACGACGA GATGAAGGAA ACCATTGGTG AACCGCCAAT GTCCAAGCTC 
ATTTCCAAAG CTTCCAGCTC TTCCTCCGAG GATAAATCTG GCCAGTATGG TGACCAGCGC 
GGAGGCTCTC AGACTCAAAG CAGCAAGTGG ACCCCGGTGG GTCCGGCCTC AGGAGGTGGC 
AACTCTTCAT CGTCTTCTCA GAAACGCTCA ACTGTGCAGA GCGTCAGCCA GAAGAGCAGC 
TCCAGCGGGC AGCGGCACGA GCGGGACTAC AGCGGCAGCA AGAAATCCAG CAAGCACAGC 
AACGAGCACA AGTCCCACTC CAGTTCCAGC CCGGCCAAAG TCTCCAGCAA CCACTCGCGC 
CGAGTCCCCA AGTCCCCCAG AGACAGAGAG GCCAATTGGG ACTCGCCCTC CATCCCGCAT 
TCCTTCCCCA GTGGACATCC CAACCAAACC TTCCCACCCT CGCTCATCAA ATCCAGCTCC 
ATGCCCCAGA AGCCCACCGC TTACGTTCGG CCCATGGACG GCCAGGAGAC GACCGAGGCA 
AAGACGTCTT CAGATTCGTA CAGCGGCCAA TCGCACAGCA GCACAGTTGG AGAAATGAAG 
CCCAATGGAA AGGGCCCACT CCCCAAGCTC AAGATCCCAA CACAGCCTCC AGAGGCGGGG 
GATGCGAGCT GTGTTGATGA GATTTTAAAG GAAATGACTC AGGTCTGGCC TCCTCCATTA 
ACAGCCATTC ACACCCCCTG CAAGACCGAG CCGCCTTCCA AATTCCCCTT CTCCAACGCT 
AAGGATGCCC AGCACCAGTC TGTTGGGGCT CAAAAAAAGT TCTCAGGGAA GAGCGCATCA 
ACCAGTCACC CGTCAAAGCC ATGTGACAGC GAGCAGTCCA ACATGCTTCA TGATGATCTT 
AAGCTCAGCA GCAGTGAAGA CAGTGACGGA GAGCAAGATC CTGCCAAAAA CACCCCTCAA 
AACAACTCCA CAAGTAACAA CAGTAATAAC AGTGAGGGAG CGGAGCGAGA GGACACCAGC 
AGTCACAGCG GCTCCGAGAG CAGCTCCGGG TCCGACAGCG AGAGCGAGAG CAGCTCCACC 
GACAGCGAGA CCACCGAACC GCCACGAGCT GCGACCCCCG AGCGGGAACA GCCTACGGCT 
AACAAATGGC TTCTGGACAA CTGGTTAAAT AAGGTCAACA AGCTGTCTCC TGCTTCTCCG 
GTGGAAAACA GCAGCAGCAG CACGCCGGGA ACCAAGTATA AGAAGGAGGG TCGAGATACC 
AGCTCCAGCA GGAGTTACAG TGGGCAGGGG TCTAAAGAAG CTGCAACGTC AGCCGGCCGA 
GAGTTGCGAC CCAATCAGAA GAGCTCCGAA AGCTGGCGAG GACGCAGTCA GAAATCTCCT 
GCTCAGAGCG AGGGCACAAG TCAATCCGGG CGACGGGTCG TAGGCAAAAA ACAGCCCAAA 
AAACCTGAGA AGCCGCCGGT GGTTGAAGAG CCCAAGGGTG GACTAAAAGT AGAAACCGAA 
CCATCGCCGG AAATCCCGTC ACACAGAGCT CGCGCTCCCA ATAAAGGCCT GCGCAAACCC 
AACATTAAAA AAGAACCCAA AACCTCCTCT CCACGACCTC CGCCTGATAA GCGCAAGACG 
AAAGCGGCAA AAGTGCCCAG CAAGTCTCGC GAGTTCATCG AATCGGATTC GTCGACGTCG 
GATTCTGAAG GCAACGAAAG CGACCCATTG CCTTCTCAGA CGCCGAAAGA TTACAGCGAA 
AACCCGTTGG TGCCACTCTC GCCCATTTAT GACCCGCCCA GACTGCTGGT GCAGATCAAT 
CTCAGCCTGC TGACTAGAGT CCCGGGACAG CTCTACAATG AGACGGAGGT TAAGGTGGAG 
CGAGGGTCTC CAGCGGAGGG AAAAAGCAAC CAGAAGCAGC CCGGAGAAAA GATAAGCAAG 
GAAGGAAGGA AGAGGAAACA CAAGAATGAA GATGATGGTG TGAAACCAGA GAGTAAGAAG 
TCCAAACTGG ATGAGAAGTC TTCACGCAAA TCAAGCAGTA ATGATTCTTC CAAGCGTGCT 
CAGGAAAAAG AGAAGGAGTC TGCGCCGTCT CCCTCCATCA CACTGCAGCG GACGCCCAAA 
TCTGAGCAGC AGGGGCGCAA GCGGACCCTC AGCCAGTCCT CCAGCACCGT CCCCAGCAAA 
GACAAGAGCA GCTCCAGCTC CAGACACCGC AGAGGAGACG ACAAACCCCA GCGCAGAGAG 
CCCAAGGAGA AAAGCTCCAA ATCAGAGAAT CCCGTTTCAG TGCCGCCGCT CTCTGAGGGC 
TCCAAATCTA GATCTAAACT TCTGTTTGAG GACAGGGTTC ATTCGGCTGA TCATTACCTT 
CAAGAAGCCA AGAAGTTGAA ACACAATGCG GATGCTCTGA TGGACAGATT TGAGAAGGCG 
GTGTATTACC TGGACGCCGT GGTGTCTTTC ATCGAGTGTG GAAATGCCCT GGAGAAAAAC 
GCCCAGGAGG CCAAATCCCC CTTTCCCATG TATGCAGAAA CCGTGGAGCT CATCAAGTAC 
ACTATGAAGC TAAAGAGCTA CATGGCACCA GATGCAACTT CTCAAGACAA AAGGCTAGCA 
GTGCTATGTC TTCGATGTCA AGCGCTCCTG TATTTGAGGC TTTTCAAGCT GCGGAAAGAA 
AGTGCACTGA AATACTCTAA AACACTCACT GAACACTTGA AGAATTCATT GAGTAATACC 
CAAGCTCCGT CTCCTGGAGT AGCAAATAAA AGCGCAGGGA TGCCGTCTCC GGTCTCCCCC 
AAACTCTCTC CTGGCAGTGC GGGCAGCTAC TCCTCCAGCA GCTCCAGCCA AAGCGCCAGC 
TCCTCCGTCA CCATCCCGCA GCGCATCCAC CAGATGGCGG CAAGCTACGT GCAAGTCACC 
TCCAACTTCC TGTACGCCAC CGAGGTGTGG GACCAGGCTG AGCAGCTCGC CAAAGAGCAG 
AGAGAGTTCT TCACTGAGCT GGACAAGGCG ATGGGTCCGC TGATCTTCAA CACCAGCAGC 
ATGACTGACC TGGTGCGGTT CACGCGGCAG GGCCTGCACT GGCTACGACT GGACGCCAAG 
CTCATCCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005173957.1
CDS	476..3727
Translation	MASQSGNMNREDRNVLRMKERERRNQEIQQGGEAFPANSPLFPEPIKVAKEDKLSSRIQSMLGNYDEMKETIGEPPMSKLISKASSSSSEDKSGQYGDQRGGSQTQSSKWTPVGPASGGGNSSSSSQKRSTVQSVSQKSSSSGQRHERDYSGSKKSSKHSNEHKSHSSSSPAKVSSNHSRRVPKSPRDREANWDSPSIPHSFPSGHPNQTFPPSLIKSSSMPQKPTAYVRPMDGQETTEAKTSSDSYSGQSHSSTVGEMKPNGKGPLPKLKIPTQPPEAGDASCVDEILKEMTQVWPPPLTAIHTPCKTEPPSKFPFSNAKDAQHQSVGAQKKFSGKSASTSHPSKPCDSEQSNMLHDDLKLSSSEDSDGEQDPAKNTPQNNSTSNNSNNSEGAEREDTSSHSGSESSSGSDSESESSSTDSETTEPPRAATPEREQPTANKWLLDNWLNKVNKLSPASPVENSSSSTPGTKYKKEGRDTSSSRSYSGQGSKEAATSAGRELRPNQKSSESWRGRSQKSPAQSEGTSQSGRRVVGKKQPKKPEKPPVVEEPKGGLKVETEPSPEIPSHRARAPNKGLRKPNIKKEPKTSSPRPPPDKRKTKAAKVPSKSREFIESDSSTSDSEGNESDPLPSQTPKDYSENPLVPLSPIYDPPRLLVQINLSLLTRVPGQLYNETEVKVERGSPAEGKSNQKQPGEKISKEGRKRKHKNEDDGVKPESKKSKLDEKSSRKSSSNDSSKRAQEKEKESAPSPSITLQRTPKSEQQGRKRTLSQSSSTVPSKDKSSSSSRHRRGDDKPQRREPKEKSSKSENPVSVPPLSEGSKSRSKLLFEDRVHSADHYLQEAKKLKHNADALMDRFEKAVYYLDAVVSFIECGNALEKNAQEAKSPFPMYAETVELIKYTMKLKSYMAPDATSQDKRLAVLCLRCQALLYLRLFKLRKESALKYSKTLTEHLKNSLSNTQAPSPGVANKSAGMPSPVSPKLSPGSAGSYSSSSSSQSASSSVTIPQRIHQMAASYVQVTSNFLYATEVWDQAEQLAKEQREFFTELDKAMGPLIFNTSSMTDLVRFTRQGLHWLRLDAKLIQ

Target ORF information:

RefSeq Version	XM_005173900.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio AF4/FMR2 family, member 4 (aff4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173900.4

ORF Insert Sequence:

ATGGCCTCTC AGTCAGGCAA CATGAACCGT GAAGACCGGA ATGTGCTCCG TATGAAAGAA 
AGGGAAAGGA GAAATCAAGA AATCCAGCAG GGAGGAGAGG CCTTTCCAGC TAACTCCCCC 
CTCTTTCCTG AACCTATTAA AGTTGCCAAG GAAGATAAAC TCTCAAGTCG CATCCAGAGC 
ATGCTGGGAA ATTACGACGA GATGAAGGAA ACCATTGGTG AACCGCCAAT GTCCAAGCTC 
ATTTCCAAAG CTTCCAGCTC TTCCTCCGAG GATAAATCTG GCCAGTATGG TGACCAGCGC 
GGAGGCTCTC AGACTCAAAG CAGCAAGTGG ACCCCGGTGG GTCCGGCCTC AGGAGGTGGC 
AACTCTTCAT CGTCTTCTCA GAAACGCTCA ACTGTGCAGA GCGTCAGCCA GAAGAGCAGC 
TCCAGCGGGC AGCGGCACGA GCGGGACTAC AGCGGCAGCA AGAAATCCAG CAAGCACAGC 
AACGAGCACA AGTCCCACTC CAGTTCCAGC CCGGCCAAAG TCTCCAGCAA CCACTCGCGC 
CGAGTCCCCA AGTCCCCCAG AGACAGAGAG GCCAATTGGG ACTCGCCCTC CATCCCGCAT 
TCCTTCCCCA GTGGACATCC CAACCAAACC TTCCCACCCT CGCTCATCAA ATCCAGCTCC 
ATGCCCCAGA AGCCCACCGC TTACGTTCGG CCCATGGACG GCCAGGAGAC GACCGAGGCA 
AAGACGTCTT CAGATTCGTA CAGCGGCCAA TCGCACAGCA GCACAGTTGG AGAAATGAAG 
CCCAATGGAA AGGGCCCACT CCCCAAGCTC AAGATCCCAA CACAGCCTCC AGAGGCGGGG 
GATGCGAGCT GTGTTGATGA GATTTTAAAG GAAATGACTC AGGTCTGGCC TCCTCCATTA 
ACAGCCATTC ACACCCCCTG CAAGACCGAG CCGCCTTCCA AATTCCCCTT CTCCAACGCT 
AAGGATGCCC AGCACCAGTC TGTTGGGGCT CAAAAAAAGT TCTCAGGGAA GAGCGCATCA 
ACCAGTCACC CGTCAAAGCC ATGTGACAGC GAGCAGTCCA ACATGCTTCA TGATGATCTT 
AAGCTCAGCA GCAGTGAAGA CAGTGACGGA GAGCAAGATC CTGCCAAAAA CACCCCTCAA 
AACAACTCCA CAAGTAACAA CAGTAATAAC AGTGAGGGAG CGGAGCGAGA GGACACCAGC 
AGTCACAGCG GCTCCGAGAG CAGCTCCGGG TCCGACAGCG AGAGCGAGAG CAGCTCCACC 
GACAGCGAGA CCACCGAACC GCCACGAGCT GCGACCCCCG AGCGGGAACA GCCTACGGCT 
AACAAATGGC TTCTGGACAA CTGGTTAAAT AAGGTCAACA AGCTGTCTCC TGCTTCTCCG 
GTGGAAAACA GCAGCAGCAG CACGCCGGGA ACCAAGTATA AGAAGGAGGG TCGAGATACC 
AGCTCCAGCA GGAGTTACAG TGGGCAGGGG TCTAAAGAAG CTGCAACGTC AGCCGGCCGA 
GAGTTGCGAC CCAATCAGAA GAGCTCCGAA AGCTGGCGAG GACGCAGTCA GAAATCTCCT 
GCTCAGAGCG AGGGCACAAG TCAATCCGGG CGACGGGTCG TAGGCAAAAA ACAGCCCAAA 
AAACCTGAGA AGCCGCCGGT GGTTGAAGAG CCCAAGGGTG GACTAAAAGT AGAAACCGAA 
CCATCGCCGG AAATCCCGTC ACACAGAGCT CGCGCTCCCA ATAAAGGCCT GCGCAAACCC 
AACATTAAAA AAGAACCCAA AACCTCCTCT CCACGACCTC CGCCTGATAA GCGCAAGACG 
AAAGCGGCAA AAGTGCCCAG CAAGTCTCGC GAGTTCATCG AATCGGATTC GTCGACGTCG 
GATTCTGAAG GCAACGAAAG CGACCCATTG CCTTCTCAGA CGCCGAAAGA TTACAGCGAA 
AACCCGTTGG TGCCACTCTC GCCCATTTAT GACCCGCCCA GACTGCTGGT GCAGATCAAT 
CTCAGCCTGC TGACTAGAGT CCCGGGACAG CTCTACAATG AGACGGAGGT TAAGGTGGAG 
CGAGGGTCTC CAGCGGAGGG AAAAAGCAAC CAGAAGCAGC CCGGAGAAAA GATAAGCAAG 
GAAGGAAGGA AGAGGAAACA CAAGAATGAA GATGATGGTG TGAAACCAGA GAGTAAGAAG 
TCCAAACTGG ATGAGAAGTC TTCACGCAAA TCAAGCAGTA ATGATTCTTC CAAGCGTGCT 
CAGGAAAAAG AGAAGGAGTC TGCGCCGTCT CCCTCCATCA CACTGCAGCG GACGCCCAAA 
TCTGAGCAGC AGGGGCGCAA GCGGACCCTC AGCCAGTCCT CCAGCACCGT CCCCAGCAAA 
GACAAGAGCA GCTCCAGCTC CAGACACCGC AGAGGAGACG ACAAACCCCA GCGCAGAGAG 
CCCAAGGAGA AAAGCTCCAA ATCAGAGAAT CCCGTTTCAG TGCCGCCGCT CTCTGAGGGC 
TCCAAATCTA GATCTAAACT TCTGTTTGAG GACAGGGTTC ATTCGGCTGA TCATTACCTT 
CAAGAAGCCA AGAAGTTGAA ACACAATGCG GATGCTCTGA TGGACAGATT TGAGAAGGCG 
GTGTATTACC TGGACGCCGT GGTGTCTTTC ATCGAGTGTG GAAATGCCCT GGAGAAAAAC 
GCCCAGGAGG CCAAATCCCC CTTTCCCATG TATGCAGAAA CCGTGGAGCT CATCAAGTAC 
ACTATGAAGC TAAAGAGCTA CATGGCACCA GATGCAACTT CTCAAGACAA AAGGCTAGCA 
GTGCTATGTC TTCGATGTCA AGCGCTCCTG TATTTGAGGC TTTTCAAGCT GCGGAAAGAA 
AGTGCACTGA AATACTCTAA AACACTCACT GAACACTTGA AGAATTCATT GAGTAATACC 
CAAGCTCCGT CTCCTGGAGT AGCAAATAAA AGCGCAGGGA TGCCGTCTCC GGTCTCCCCC 
AAACTCTCTC CTGGCAGTGC GGGCAGCTAC TCCTCCAGCA GCTCCAGCCA AAGCGCCAGC 
TCCTCCGTCA CCATCCCGCA GCGCATCCAC CAGATGGCGG CAAGCTACGT GCAAGTCACC 
TCCAACTTCC TGTACGCCAC CGAGGTGTGG GACCAGGCTG AGCAGCTCGC CAAAGAGCAG 
AGAGAGTTCT TCACTGAGCT GGACAAGGCG ATGGGTCCGC TGATCTTCAA CACCAGCAGC 
ATGACTGACC TGGTGCGGTT CACGCGGCAG GGCCTGCACT GGCTACGACT GGACGCCAAG 
CTCATCCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF4 ( XM_005173900.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005173957.1 Danio rerio AF4/FMR2 family, member 4 (aff4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa26233
Clone ID Related Accession (Same CDS sequence)	XM_005173900.4 , XM_005173900.3
Accession Version	XM_005173900.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	AF4/FMR2 family member 4 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173900.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCTC AGTCAGGCAA CATGAACCGT GAAGACCGGA ATGTGCTCCG TATGAAAGAA 
AGGGAAAGGA GAAATCAAGA AATCCAGCAG GGAGGAGAGG CCTTTCCAGC TAACTCCCCC 
CTCTTTCCTG AACCTATTAA AGTTGCCAAG GAAGATAAAC TCTCAAGTCG CATCCAGAGC 
ATGCTGGGAA ATTACGACGA GATGAAGGAA ACCATTGGTG AACCGCCAAT GTCCAAGCTC 
ATTTCCAAAG CTTCCAGCTC TTCCTCCGAG GATAAATCTG GCCAGTATGG TGACCAGCGC 
GGAGGCTCTC AGACTCAAAG CAGCAAGTGG ACCCCGGTGG GTCCGGCCTC AGGAGGTGGC 
AACTCTTCAT CGTCTTCTCA GAAACGCTCA ACTGTGCAGA GCGTCAGCCA GAAGAGCAGC 
TCCAGCGGGC AGCGGCACGA GCGGGACTAC AGCGGCAGCA AGAAATCCAG CAAGCACAGC 
AACGAGCACA AGTCCCACTC CAGTTCCAGC CCGGCCAAAG TCTCCAGCAA CCACTCGCGC 
CGAGTCCCCA AGTCCCCCAG AGACAGAGAG GCCAATTGGG ACTCGCCCTC CATCCCGCAT 
TCCTTCCCCA GTGGACATCC CAACCAAACC TTCCCACCCT CGCTCATCAA ATCCAGCTCC 
ATGCCCCAGA AGCCCACCGC TTACGTTCGG CCCATGGACG GCCAGGAGAC GACCGAGGCA 
AAGACGTCTT CAGATTCGTA CAGCGGCCAA TCGCACAGCA GCACAGTTGG AGAAATGAAG 
CCCAATGGAA AGGGCCCACT CCCCAAGCTC AAGATCCCAA CACAGCCTCC AGAGGCGGGG 
GATGCGAGCT GTGTTGATGA GATTTTAAAG GAAATGACTC AGGTCTGGCC TCCTCCATTA 
ACAGCCATTC ACACCCCCTG CAAGACCGAG CCGCCTTCCA AATTCCCCTT CTCCAACGCT 
AAGGATGCCC AGCACCAGTC TGTTGGGGCT CAAAAAAAGT TCTCAGGGAA GAGCGCATCA 
ACCAGTCACC CGTCAAAGCC ATGTGACAGC GAGCAGTCCA ACATGCTTCA TGATGATCTT 
AAGCTCAGCA GCAGTGAAGA CAGTGACGGA GAGCAAGATC CTGCCAAAAA CACCCCTCAA 
AACAACTCCA CAAGTAACAA CAGTAATAAC AGTGAGGGAG CGGAGCGAGA GGACACCAGC 
AGTCACAGCG GCTCCGAGAG CAGCTCCGGG TCCGACAGCG AGAGCGAGAG CAGCTCCACC 
GACAGCGAGA CCACCGAACC GCCACGAGCT GCGACCCCCG AGCGGGAACA GCCTACGGCT 
AACAAATGGC TTCTGGACAA CTGGTTAAAT AAGGTCAACA AGCTGTCTCC TGCTTCTCCG 
GTGGAAAACA GCAGCAGCAG CACGCCGGGA ACCAAGTATA AGAAGGAGGG TCGAGATACC 
AGCTCCAGCA GGAGTTACAG TGGGCAGGGG TCTAAAGAAG CTGCAACGTC AGCCGGCCGA 
GAGTTGCGAC CCAATCAGAA GAGCTCCGAA AGCTGGCGAG GACGCAGTCA GAAATCTCCT 
GCTCAGAGCG AGGGCACAAG TCAATCCGGG CGACGGGTCG TAGGCAAAAA ACAGCCCAAA 
AAACCTGAGA AGCCGCCGGT GGTTGAAGAG CCCAAGGGTG GACTAAAAGT AGAAACCGAA 
CCATCGCCGG AAATCCCGTC ACACAGAGCT CGCGCTCCCA ATAAAGGCCT GCGCAAACCC 
AACATTAAAA AAGAACCCAA AACCTCCTCT CCACGACCTC CGCCTGATAA GCGCAAGACG 
AAAGCGGCAA AAGTGCCCAG CAAGTCTCGC GAGTTCATCG AATCGGATTC GTCGACGTCG 
GATTCTGAAG GCAACGAAAG CGACCCATTG CCTTCTCAGA CGCCGAAAGA TTACAGCGAA 
AACCCGTTGG TGCCACTCTC GCCCATTTAT GACCCGCCCA GACTGCTGGT GCAGATCAAT 
CTCAGCCTGC TGACTAGAGT CCCGGGACAG CTCTACAATG AGACGGAGGT TAAGGTGGAG 
CGAGGGTCTC CAGCGGAGGG AAAAAGCAAC CAGAAGCAGC CCGGAGAAAA GATAAGCAAG 
GAAGGAAGGA AGAGGAAACA CAAGAATGAA GATGATGGTG TGAAACCAGA GAGTAAGAAG 
TCCAAACTGG ATGAGAAGTC TTCACGCAAA TCAAGCAGTA ATGATTCTTC CAAGCGTGCT 
CAGGAAAAAG AGAAGGAGTC TGCGCCGTCT CCCTCCATCA CACTGCAGCG GACGCCCAAA 
TCTGAGCAGC AGGGGCGCAA GCGGACCCTC AGCCAGTCCT CCAGCACCGT CCCCAGCAAA 
GACAAGAGCA GCTCCAGCTC CAGACACCGC AGAGGAGACG ACAAACCCCA GCGCAGAGAG 
CCCAAGGAGA AAAGCTCCAA ATCAGAGAAT CCCGTTTCAG TGCCGCCGCT CTCTGAGGGC 
TCCAAATCTA GATCTAAACT TCTGTTTGAG GACAGGGTTC ATTCGGCTGA TCATTACCTT 
CAAGAAGCCA AGAAGTTGAA ACACAATGCG GATGCTCTGA TGGACAGATT TGAGAAGGCG 
GTGTATTACC TGGACGCCGT GGTGTCTTTC ATCGAGTGTG GAAATGCCCT GGAGAAAAAC 
GCCCAGGAGG CCAAATCCCC CTTTCCCATG TATGCAGAAA CCGTGGAGCT CATCAAGTAC 
ACTATGAAGC TAAAGAGCTA CATGGCACCA GATGCAACTT CTCAAGACAA AAGGCTAGCA 
GTGCTATGTC TTCGATGTCA AGCGCTCCTG TATTTGAGGC TTTTCAAGCT GCGGAAAGAA 
AGTGCACTGA AATACTCTAA AACACTCACT GAACACTTGA AGAATTCATT GAGTAATACC 
CAAGCTCCGT CTCCTGGAGT AGCAAATAAA AGCGCAGGGA TGCCGTCTCC GGTCTCCCCC 
AAACTCTCTC CTGGCAGTGC GGGCAGCTAC TCCTCCAGCA GCTCCAGCCA AAGCGCCAGC 
TCCTCCGTCA CCATCCCGCA GCGCATCCAC CAGATGGCGG CAAGCTACGT GCAAGTCACC 
TCCAACTTCC TGTACGCCAC CGAGGTGTGG GACCAGGCTG AGCAGCTCGC CAAAGAGCAG 
AGAGAGTTCT TCACTGAGCT GGACAAGGCG ATGGGTCCGC TGATCTTCAA CACCAGCAGC 
ATGACTGACC TGGTGCGGTT CACGCGGCAG GGCCTGCACT GGCTACGACT GGACGCCAAG 
CTCATCCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005173957.1
CDS	528..3779
Translation	MASQSGNMNREDRNVLRMKERERRNQEIQQGGEAFPANSPLFPEPIKVAKEDKLSSRIQSMLGNYDEMKETIGEPPMSKLISKASSSSSEDKSGQYGDQRGGSQTQSSKWTPVGPASGGGNSSSSSQKRSTVQSVSQKSSSSGQRHERDYSGSKKSSKHSNEHKSHSSSSPAKVSSNHSRRVPKSPRDREANWDSPSIPHSFPSGHPNQTFPPSLIKSSSMPQKPTAYVRPMDGQETTEAKTSSDSYSGQSHSSTVGEMKPNGKGPLPKLKIPTQPPEAGDASCVDEILKEMTQVWPPPLTAIHTPCKTEPPSKFPFSNAKDAQHQSVGAQKKFSGKSASTSHPSKPCDSEQSNMLHDDLKLSSSEDSDGEQDPAKNTPQNNSTSNNSNNSEGAEREDTSSHSGSESSSGSDSESESSSTDSETTEPPRAATPEREQPTANKWLLDNWLNKVNKLSPASPVENSSSSTPGTKYKKEGRDTSSSRSYSGQGSKEAATSAGRELRPNQKSSESWRGRSQKSPAQSEGTSQSGRRVVGKKQPKKPEKPPVVEEPKGGLKVETEPSPEIPSHRARAPNKGLRKPNIKKEPKTSSPRPPPDKRKTKAAKVPSKSREFIESDSSTSDSEGNESDPLPSQTPKDYSENPLVPLSPIYDPPRLLVQINLSLLTRVPGQLYNETEVKVERGSPAEGKSNQKQPGEKISKEGRKRKHKNEDDGVKPESKKSKLDEKSSRKSSSNDSSKRAQEKEKESAPSPSITLQRTPKSEQQGRKRTLSQSSSTVPSKDKSSSSSRHRRGDDKPQRREPKEKSSKSENPVSVPPLSEGSKSRSKLLFEDRVHSADHYLQEAKKLKHNADALMDRFEKAVYYLDAVVSFIECGNALEKNAQEAKSPFPMYAETVELIKYTMKLKSYMAPDATSQDKRLAVLCLRCQALLYLRLFKLRKESALKYSKTLTEHLKNSLSNTQAPSPGVANKSAGMPSPVSPKLSPGSAGSYSSSSSSQSASSSVTIPQRIHQMAASYVQVTSNFLYATEVWDQAEQLAKEQREFFTELDKAMGPLIFNTSSMTDLVRFTRQGLHWLRLDAKLIQ

Target ORF information:

RefSeq Version	XM_005173900.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio AF4/FMR2 family, member 4 (aff4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173900.3

ORF Insert Sequence:

ATGGCCTCTC AGTCAGGCAA CATGAACCGT GAAGACCGGA ATGTGCTCCG TATGAAAGAA 
AGGGAAAGGA GAAATCAAGA AATCCAGCAG GGAGGAGAGG CCTTTCCAGC TAACTCCCCC 
CTCTTTCCTG AACCTATTAA AGTTGCCAAG GAAGATAAAC TCTCAAGTCG CATCCAGAGC 
ATGCTGGGAA ATTACGACGA GATGAAGGAA ACCATTGGTG AACCGCCAAT GTCCAAGCTC 
ATTTCCAAAG CTTCCAGCTC TTCCTCCGAG GATAAATCTG GCCAGTATGG TGACCAGCGC 
GGAGGCTCTC AGACTCAAAG CAGCAAGTGG ACCCCGGTGG GTCCGGCCTC AGGAGGTGGC 
AACTCTTCAT CGTCTTCTCA GAAACGCTCA ACTGTGCAGA GCGTCAGCCA GAAGAGCAGC 
TCCAGCGGGC AGCGGCACGA GCGGGACTAC AGCGGCAGCA AGAAATCCAG CAAGCACAGC 
AACGAGCACA AGTCCCACTC CAGTTCCAGC CCGGCCAAAG TCTCCAGCAA CCACTCGCGC 
CGAGTCCCCA AGTCCCCCAG AGACAGAGAG GCCAATTGGG ACTCGCCCTC CATCCCGCAT 
TCCTTCCCCA GTGGACATCC CAACCAAACC TTCCCACCCT CGCTCATCAA ATCCAGCTCC 
ATGCCCCAGA AGCCCACCGC TTACGTTCGG CCCATGGACG GCCAGGAGAC GACCGAGGCA 
AAGACGTCTT CAGATTCGTA CAGCGGCCAA TCGCACAGCA GCACAGTTGG AGAAATGAAG 
CCCAATGGAA AGGGCCCACT CCCCAAGCTC AAGATCCCAA CACAGCCTCC AGAGGCGGGG 
GATGCGAGCT GTGTTGATGA GATTTTAAAG GAAATGACTC AGGTCTGGCC TCCTCCATTA 
ACAGCCATTC ACACCCCCTG CAAGACCGAG CCGCCTTCCA AATTCCCCTT CTCCAACGCT 
AAGGATGCCC AGCACCAGTC TGTTGGGGCT CAAAAAAAGT TCTCAGGGAA GAGCGCATCA 
ACCAGTCACC CGTCAAAGCC ATGTGACAGC GAGCAGTCCA ACATGCTTCA TGATGATCTT 
AAGCTCAGCA GCAGTGAAGA CAGTGACGGA GAGCAAGATC CTGCCAAAAA CACCCCTCAA 
AACAACTCCA CAAGTAACAA CAGTAATAAC AGTGAGGGAG CGGAGCGAGA GGACACCAGC 
AGTCACAGCG GCTCCGAGAG CAGCTCCGGG TCCGACAGCG AGAGCGAGAG CAGCTCCACC 
GACAGCGAGA CCACCGAACC GCCACGAGCT GCGACCCCCG AGCGGGAACA GCCTACGGCT 
AACAAATGGC TTCTGGACAA CTGGTTAAAT AAGGTCAACA AGCTGTCTCC TGCTTCTCCG 
GTGGAAAACA GCAGCAGCAG CACGCCGGGA ACCAAGTATA AGAAGGAGGG TCGAGATACC 
AGCTCCAGCA GGAGTTACAG TGGGCAGGGG TCTAAAGAAG CTGCAACGTC AGCCGGCCGA 
GAGTTGCGAC CCAATCAGAA GAGCTCCGAA AGCTGGCGAG GACGCAGTCA GAAATCTCCT 
GCTCAGAGCG AGGGCACAAG TCAATCCGGG CGACGGGTCG TAGGCAAAAA ACAGCCCAAA 
AAACCTGAGA AGCCGCCGGT GGTTGAAGAG CCCAAGGGTG GACTAAAAGT AGAAACCGAA 
CCATCGCCGG AAATCCCGTC ACACAGAGCT CGCGCTCCCA ATAAAGGCCT GCGCAAACCC 
AACATTAAAA AAGAACCCAA AACCTCCTCT CCACGACCTC CGCCTGATAA GCGCAAGACG 
AAAGCGGCAA AAGTGCCCAG CAAGTCTCGC GAGTTCATCG AATCGGATTC GTCGACGTCG 
GATTCTGAAG GCAACGAAAG CGACCCATTG CCTTCTCAGA CGCCGAAAGA TTACAGCGAA 
AACCCGTTGG TGCCACTCTC GCCCATTTAT GACCCGCCCA GACTGCTGGT GCAGATCAAT 
CTCAGCCTGC TGACTAGAGT CCCGGGACAG CTCTACAATG AGACGGAGGT TAAGGTGGAG 
CGAGGGTCTC CAGCGGAGGG AAAAAGCAAC CAGAAGCAGC CCGGAGAAAA GATAAGCAAG 
GAAGGAAGGA AGAGGAAACA CAAGAATGAA GATGATGGTG TGAAACCAGA GAGTAAGAAG 
TCCAAACTGG ATGAGAAGTC TTCACGCAAA TCAAGCAGTA ATGATTCTTC CAAGCGTGCT 
CAGGAAAAAG AGAAGGAGTC TGCGCCGTCT CCCTCCATCA CACTGCAGCG GACGCCCAAA 
TCTGAGCAGC AGGGGCGCAA GCGGACCCTC AGCCAGTCCT CCAGCACCGT CCCCAGCAAA 
GACAAGAGCA GCTCCAGCTC CAGACACCGC AGAGGAGACG ACAAACCCCA GCGCAGAGAG 
CCCAAGGAGA AAAGCTCCAA ATCAGAGAAT CCCGTTTCAG TGCCGCCGCT CTCTGAGGGC 
TCCAAATCTA GATCTAAACT TCTGTTTGAG GACAGGGTTC ATTCGGCTGA TCATTACCTT 
CAAGAAGCCA AGAAGTTGAA ACACAATGCG GATGCTCTGA TGGACAGATT TGAGAAGGCG 
GTGTATTACC TGGACGCCGT GGTGTCTTTC ATCGAGTGTG GAAATGCCCT GGAGAAAAAC 
GCCCAGGAGG CCAAATCCCC CTTTCCCATG TATGCAGAAA CCGTGGAGCT CATCAAGTAC 
ACTATGAAGC TAAAGAGCTA CATGGCACCA GATGCAACTT CTCAAGACAA AAGGCTAGCA 
GTGCTATGTC TTCGATGTCA AGCGCTCCTG TATTTGAGGC TTTTCAAGCT GCGGAAAGAA 
AGTGCACTGA AATACTCTAA AACACTCACT GAACACTTGA AGAATTCATT GAGTAATACC 
CAAGCTCCGT CTCCTGGAGT AGCAAATAAA AGCGCAGGGA TGCCGTCTCC GGTCTCCCCC 
AAACTCTCTC CTGGCAGTGC GGGCAGCTAC TCCTCCAGCA GCTCCAGCCA AAGCGCCAGC 
TCCTCCGTCA CCATCCCGCA GCGCATCCAC CAGATGGCGG CAAGCTACGT GCAAGTCACC 
TCCAACTTCC TGTACGCCAC CGAGGTGTGG GACCAGGCTG AGCAGCTCGC CAAAGAGCAG 
AGAGAGTTCT TCACTGAGCT GGACAAGGCG ATGGGTCCGC TGATCTTCAA CACCAGCAGC 
ATGACTGACC TGGTGCGGTT CACGCGGCAG GGCCTGCACT GGCTACGACT GGACGCCAAG 
CTCATCCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF4 ( XM_679235.10 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_684327.6 Danio rerio AF4/FMR2 family, member 4 (aff4), transcript variant X3, mRNA.

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CloneID	ODa26234
Clone ID Related Accession (Same CDS sequence)	XM_679235.10 , XM_679235.9
Accession Version	XM_679235.10 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	AF4/FMR2 family member 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_679235.9. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCGTG AAGACCGGAA TGTGCTCCGT ATGAAAGAAA GGGAAAGGAG AAATCAAGAA 
ATCCAGCAGG GAGGAGAGGC CTTTCCAGCT AACTCCCCCC TCTTTCCTGA ACCTATTAAA 
GTTGCCAAGG AAGATAAACT CTCAAGTCGC ATCCAGAGCA TGCTGGGAAA TTACGACGAG 
ATGAAGGAAA CCATTGGTGA ACCGCCAATG TCCAAGCTCA TTTCCAAAGC TTCCAGCTCT 
TCCTCCGAGG ATAAATCTGG CCAGTATGGT GACCAGCGCG GAGGCTCTCA GACTCAAAGC 
AGCAAGTGGA CCCCGGTGGG TCCGGCCTCA GGAGGTGGCA ACTCTTCATC GTCTTCTCAG 
AAACGCTCAA CTGTGCAGAG CGTCAGCCAG AAGAGCAGCT CCAGCGGGCA GCGGCACGAG 
CGGGACTACA GCGGCAGCAA GAAATCCAGC AAGCACAGCA ACGAGCACAA GTCCCACTCC 
AGTTCCAGCC CGGCCAAAGT CTCCAGCAAC CACTCGCGCC GAGTCCCCAA GTCCCCCAGA 
GACAGAGAGG CCAATTGGGA CTCGCCCTCC ATCCCGCATT CCTTCCCCAG TGGACATCCC 
AACCAAACCT TCCCACCCTC GCTCATCAAA TCCAGCTCCA TGCCCCAGAA GCCCACCGCT 
TACGTTCGGC CCATGGACGG CCAGGAGACG ACCGAGGCAA AGACGTCTTC AGATTCGTAC 
AGCGGCCAAT CGCACAGCAG CACAGTTGGA GAAATGAAGC CCAATGGAAA GGGCCCACTC 
CCCAAGCTCA AGATCCCAAC ACAGCCTCCA GAGGCGGGGG ATGCGAGCTG TGTTGATGAG 
ATTTTAAAGG AAATGACTCA GGTCTGGCCT CCTCCATTAA CAGCCATTCA CACCCCCTGC 
AAGACCGAGC CGCCTTCCAA ATTCCCCTTC TCCAACGCTA AGGATGCCCA GCACCAGTCT 
GTTGGGGCTC AAAAAAAGTT CTCAGGGAAG AGCGCATCAA CCAGTCACCC GTCAAAGCCA 
TGTGACAGCG AGCAGTCCAA CATGCTTCAT GATGATCTTA AGCTCAGCAG CAGTGAAGAC 
AGTGACGGAG AGCAAGATCC TGCCAAAAAC ACCCCTCAAA ACAACTCCAC AAGTAACAAC 
AGTAATAACA GTGAGGGAGC GGAGCGAGAG GACACCAGCA GTCACAGCGG CTCCGAGAGC 
AGCTCCGGGT CCGACAGCGA GAGCGAGAGC AGCTCCACCG ACAGCGAGAC CACCGAACCG 
CCACGAGCTG CGACCCCCGA GCGGGAACAG CCTACGGCTA ACAAATGGCT TCTGGACAAC 
TGGTTAAATA AGGTCAACAA GCTGTCTCCT GCTTCTCCGG TGGAAAACAG CAGCAGCAGC 
ACGCCGGGAA CCAAGTATAA GAAGGAGGGT CGAGATACCA GCTCCAGCAG GAGTTACAGT 
GGGCAGGGGT CTAAAGAAGC TGCAACGTCA GCCGGCCGAG AGTTGCGACC CAATCAGAAG 
AGCTCCGAAA GCTGGCGAGG ACGCAGTCAG AAATCTCCTG CTCAGAGCGA GGGCACAAGT 
CAATCCGGGC GACGGGTCGT AGGCAAAAAA CAGCCCAAAA AACCTGAGAA GCCGCCGGTG 
GTTGAAGAGC CCAAGGGTGG ACTAAAAGTA GAAACCGAAC CATCGCCGGA AATCCCGTCA 
CACAGAGCTC GCGCTCCCAA TAAAGGCCTG CGCAAACCCA ACATTAAAAA AGAACCCAAA 
ACCTCCTCTC CACGACCTCC GCCTGATAAG CGCAAGACGA AAGCGGCAAA AGTGCCCAGC 
AAGTCTCGCG AGTTCATCGA ATCGGATTCG TCGACGTCGG ATTCTGAAGG CAACGAAAGC 
GACCCATTGC CTTCTCAGAC GCCGAAAGAT TACAGCGAAA ACCCGTTGGT GCCACTCTCG 
CCCATTTATG ACCCGCCCAG ACTGCTGGTG CAGATCAATC TCAGCCTGCT GACTAGAGTC 
CCGGGACAGC TCTACAATGA GACGGAGGTT AAGGTGGAGC GAGGGTCTCC AGCGGAGGGA 
AAAAGCAACC AGAAGCAGCC CGGAGAAAAG ATAAGCAAGG AAGGAAGGAA GAGGAAACAC 
AAGAATGAAG ATGATGGTGT GAAACCAGAG AGTAAGAAGT CCAAACTGGA TGAGAAGTCT 
TCACGCAAAT CAAGCAGTAA TGATTCTTCC AAGCGTGCTC AGGAAAAAGA GAAGGAGTCT 
GCGCCGTCTC CCTCCATCAC ACTGCAGCGG ACGCCCAAAT CTGAGCAGCA GGGGCGCAAG 
CGGACCCTCA GCCAGTCCTC CAGCACCGTC CCCAGCAAAG ACAAGAGCAG CTCCAGCTCC 
AGACACCGCA GAGGAGACGA CAAACCCCAG CGCAGAGAGC CCAAGGAGAA AAGCTCCAAA 
TCAGAGAATC CCGTTTCAGT GCCGCCGCTC TCTGAGGGCT CCAAATCTAG ATCTAAACTT 
CTGTTTGAGG ACAGGGTTCA TTCGGCTGAT CATTACCTTC AAGAAGCCAA GAAGTTGAAA 
CACAATGCGG ATGCTCTGGT GAGGATGGAC AGATTTGAGA AGGCGGTGTA TTACCTGGAC 
GCCGTGGTGT CTTTCATCGA GTGTGGAAAT GCCCTGGAGA AAAACGCCCA GGAGGCCAAA 
TCCCCCTTTC CCATGTATGC AGAAACCGTG GAGCTCATCA AGTACACTAT GAAGCTAAAG 
AGCTACATGG CACCAGATGC AACTTCTCAA GACAAAAGGC TAGCAGTGCT ATGTCTTCGA 
TGTCAAGCGC TCCTGTATTT GAGGCTTTTC AAGCTGCGGA AAGAAAGTGC ACTGAAATAC 
TCTAAAACAC TCACTGAACA CTTGAAGAAT TCATTGAGTA ATACCCAAGC TCCGTCTCCT 
GGAGTAGCAA ATAAAAGCGC AGGGATGCCG TCTCCGGTCT CCCCCAAACT CTCTCCTGGC 
AGTGCGGGCA GCTACTCCTC CAGCAGCTCC AGCCAAAGCG CCAGCTCCTC CGTCACCATC 
CCGCAGCGCA TCCACCAGAT GGCGGCAAGC TACGTGCAAG TCACCTCCAA CTTCCTGTAC 
GCCACCGAGG TGTGGGACCA GGCTGAGCAG CTCGCCAAAG AGCAGAGAGA GTTCTTCACT 
GAGCTGGACA AGGCGATGGG TCCGCTGATC TTCAACACCA GCAGCATGAC TGACCTGGTG 
CGGTTCACGC GGCAGGGCCT GCACTGGCTA CGACTGGACG CCAAGCTCAT CCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_684327.6
CDS	300..3536
Translation	MNREDRNVLRMKERERRNQEIQQGGEAFPANSPLFPEPIKVAKEDKLSSRIQSMLGNYDEMKETIGEPPMSKLISKASSSSSEDKSGQYGDQRGGSQTQSSKWTPVGPASGGGNSSSSSQKRSTVQSVSQKSSSSGQRHERDYSGSKKSSKHSNEHKSHSSSSPAKVSSNHSRRVPKSPRDREANWDSPSIPHSFPSGHPNQTFPPSLIKSSSMPQKPTAYVRPMDGQETTEAKTSSDSYSGQSHSSTVGEMKPNGKGPLPKLKIPTQPPEAGDASCVDEILKEMTQVWPPPLTAIHTPCKTEPPSKFPFSNAKDAQHQSVGAQKKFSGKSASTSHPSKPCDSEQSNMLHDDLKLSSSEDSDGEQDPAKNTPQNNSTSNNSNNSEGAEREDTSSHSGSESSSGSDSESESSSTDSETTEPPRAATPEREQPTANKWLLDNWLNKVNKLSPASPVENSSSSTPGTKYKKEGRDTSSSRSYSGQGSKEAATSAGRELRPNQKSSESWRGRSQKSPAQSEGTSQSGRRVVGKKQPKKPEKPPVVEEPKGGLKVETEPSPEIPSHRARAPNKGLRKPNIKKEPKTSSPRPPPDKRKTKAAKVPSKSREFIESDSSTSDSEGNESDPLPSQTPKDYSENPLVPLSPIYDPPRLLVQINLSLLTRVPGQLYNETEVKVERGSPAEGKSNQKQPGEKISKEGRKRKHKNEDDGVKPESKKSKLDEKSSRKSSSNDSSKRAQEKEKESAPSPSITLQRTPKSEQQGRKRTLSQSSSTVPSKDKSSSSSRHRRGDDKPQRREPKEKSSKSENPVSVPPLSEGSKSRSKLLFEDRVHSADHYLQEAKKLKHNADALVRMDRFEKAVYYLDAVVSFIECGNALEKNAQEAKSPFPMYAETVELIKYTMKLKSYMAPDATSQDKRLAVLCLRCQALLYLRLFKLRKESALKYSKTLTEHLKNSLSNTQAPSPGVANKSAGMPSPVSPKLSPGSAGSYSSSSSSQSASSSVTIPQRIHQMAASYVQVTSNFLYATEVWDQAEQLAKEQREFFTELDKAMGPLIFNTSSMTDLVRFTRQGLHWLRLDAKLIQ

Target ORF information:

RefSeq Version	XM_679235.10
Organism	Danio rerio(zebrafish)
Definition	Danio rerio AF4/FMR2 family, member 4 (aff4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_679235.10

ORF Insert Sequence:

ATGAACCGTG AAGACCGGAA TGTGCTCCGT ATGAAAGAAA GGGAAAGGAG AAATCAAGAA 
ATCCAGCAGG GAGGAGAGGC CTTTCCAGCT AACTCCCCCC TCTTTCCTGA ACCTATTAAA 
GTTGCCAAGG AAGATAAACT CTCAAGTCGC ATCCAGAGCA TGCTGGGAAA TTACGACGAG 
ATGAAGGAAA CCATTGGTGA ACCGCCAATG TCCAAGCTCA TTTCCAAAGC TTCCAGCTCT 
TCCTCCGAGG ATAAATCTGG CCAGTATGGT GACCAGCGCG GAGGCTCTCA GACTCAAAGC 
AGCAAGTGGA CCCCGGTGGG TCCGGCCTCA GGAGGTGGCA ACTCTTCATC GTCTTCTCAG 
AAACGCTCAA CTGTGCAGAG CGTCAGCCAG AAGAGCAGCT CCAGCGGGCA GCGGCACGAG 
CGGGACTACA GCGGCAGCAA GAAATCCAGC AAGCACAGCA ACGAGCACAA GTCCCACTCC 
AGTTCCAGCC CGGCCAAAGT CTCCAGCAAC CACTCGCGCC GAGTCCCCAA GTCCCCCAGA 
GACAGAGAGG CCAATTGGGA CTCGCCCTCC ATCCCGCATT CCTTCCCCAG TGGACATCCC 
AACCAAACCT TCCCACCCTC GCTCATCAAA TCCAGCTCCA TGCCCCAGAA GCCCACCGCT 
TACGTTCGGC CCATGGACGG CCAGGAGACG ACCGAGGCAA AGACGTCTTC AGATTCGTAC 
AGCGGCCAAT CGCACAGCAG CACAGTTGGA GAAATGAAGC CCAATGGAAA GGGCCCACTC 
CCCAAGCTCA AGATCCCAAC ACAGCCTCCA GAGGCGGGGG ATGCGAGCTG TGTTGATGAG 
ATTTTAAAGG AAATGACTCA GGTCTGGCCT CCTCCATTAA CAGCCATTCA CACCCCCTGC 
AAGACCGAGC CGCCTTCCAA ATTCCCCTTC TCCAACGCTA AGGATGCCCA GCACCAGTCT 
GTTGGGGCTC AAAAAAAGTT CTCAGGGAAG AGCGCATCAA CCAGTCACCC GTCAAAGCCA 
TGTGACAGCG AGCAGTCCAA CATGCTTCAT GATGATCTTA AGCTCAGCAG CAGTGAAGAC 
AGTGACGGAG AGCAAGATCC TGCCAAAAAC ACCCCTCAAA ACAACTCCAC AAGTAACAAC 
AGTAATAACA GTGAGGGAGC GGAGCGAGAG GACACCAGCA GTCACAGCGG CTCCGAGAGC 
AGCTCCGGGT CCGACAGCGA GAGCGAGAGC AGCTCCACCG ACAGCGAGAC CACCGAACCG 
CCACGAGCTG CGACCCCCGA GCGGGAACAG CCTACGGCTA ACAAATGGCT TCTGGACAAC 
TGGTTAAATA AGGTCAACAA GCTGTCTCCT GCTTCTCCGG TGGAAAACAG CAGCAGCAGC 
ACGCCGGGAA CCAAGTATAA GAAGGAGGGT CGAGATACCA GCTCCAGCAG GAGTTACAGT 
GGGCAGGGGT CTAAAGAAGC TGCAACGTCA GCCGGCCGAG AGTTGCGACC CAATCAGAAG 
AGCTCCGAAA GCTGGCGAGG ACGCAGTCAG AAATCTCCTG CTCAGAGCGA GGGCACAAGT 
CAATCCGGGC GACGGGTCGT AGGCAAAAAA CAGCCCAAAA AACCTGAGAA GCCGCCGGTG 
GTTGAAGAGC CCAAGGGTGG ACTAAAAGTA GAAACCGAAC CATCGCCGGA AATCCCGTCA 
CACAGAGCTC GCGCTCCCAA TAAAGGCCTG CGCAAACCCA ACATTAAAAA AGAACCCAAA 
ACCTCCTCTC CACGACCTCC GCCTGATAAG CGCAAGACGA AAGCGGCAAA AGTGCCCAGC 
AAGTCTCGCG AGTTCATCGA ATCGGATTCG TCGACGTCGG ATTCTGAAGG CAACGAAAGC 
GACCCATTGC CTTCTCAGAC GCCGAAAGAT TACAGCGAAA ACCCGTTGGT GCCACTCTCG 
CCCATTTATG ACCCGCCCAG ACTGCTGGTG CAGATCAATC TCAGCCTGCT GACTAGAGTC 
CCGGGACAGC TCTACAATGA GACGGAGGTT AAGGTGGAGC GAGGGTCTCC AGCGGAGGGA 
AAAAGCAACC AGAAGCAGCC CGGAGAAAAG ATAAGCAAGG AAGGAAGGAA GAGGAAACAC 
AAGAATGAAG ATGATGGTGT GAAACCAGAG AGTAAGAAGT CCAAACTGGA TGAGAAGTCT 
TCACGCAAAT CAAGCAGTAA TGATTCTTCC AAGCGTGCTC AGGAAAAAGA GAAGGAGTCT 
GCGCCGTCTC CCTCCATCAC ACTGCAGCGG ACGCCCAAAT CTGAGCAGCA GGGGCGCAAG 
CGGACCCTCA GCCAGTCCTC CAGCACCGTC CCCAGCAAAG ACAAGAGCAG CTCCAGCTCC 
AGACACCGCA GAGGAGACGA CAAACCCCAG CGCAGAGAGC CCAAGGAGAA AAGCTCCAAA 
TCAGAGAATC CCGTTTCAGT GCCGCCGCTC TCTGAGGGCT CCAAATCTAG ATCTAAACTT 
CTGTTTGAGG ACAGGGTTCA TTCGGCTGAT CATTACCTTC AAGAAGCCAA GAAGTTGAAA 
CACAATGCGG ATGCTCTGGT GAGGATGGAC AGATTTGAGA AGGCGGTGTA TTACCTGGAC 
GCCGTGGTGT CTTTCATCGA GTGTGGAAAT GCCCTGGAGA AAAACGCCCA GGAGGCCAAA 
TCCCCCTTTC CCATGTATGC AGAAACCGTG GAGCTCATCA AGTACACTAT GAAGCTAAAG 
AGCTACATGG CACCAGATGC AACTTCTCAA GACAAAAGGC TAGCAGTGCT ATGTCTTCGA 
TGTCAAGCGC TCCTGTATTT GAGGCTTTTC AAGCTGCGGA AAGAAAGTGC ACTGAAATAC 
TCTAAAACAC TCACTGAACA CTTGAAGAAT TCATTGAGTA ATACCCAAGC TCCGTCTCCT 
GGAGTAGCAA ATAAAAGCGC AGGGATGCCG TCTCCGGTCT CCCCCAAACT CTCTCCTGGC 
AGTGCGGGCA GCTACTCCTC CAGCAGCTCC AGCCAAAGCG CCAGCTCCTC CGTCACCATC 
CCGCAGCGCA TCCACCAGAT GGCGGCAAGC TACGTGCAAG TCACCTCCAA CTTCCTGTAC 
GCCACCGAGG TGTGGGACCA GGCTGAGCAG CTCGCCAAAG AGCAGAGAGA GTTCTTCACT 
GAGCTGGACA AGGCGATGGG TCCGCTGATC TTCAACACCA GCAGCATGAC TGACCTGGTG 
CGGTTCACGC GGCAGGGCCT GCACTGGCTA CGACTGGACG CCAAGCTCAT CCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF4 ( XM_679235.9 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_684327.6 Danio rerio AF4/FMR2 family, member 4 (aff4), transcript variant X3, mRNA.

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CloneID	ODa26234
Clone ID Related Accession (Same CDS sequence)	XM_679235.10 , XM_679235.9
Accession Version	XM_679235.9	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	AF4/FMR2 family member 4 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_679235.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCGTG AAGACCGGAA TGTGCTCCGT ATGAAAGAAA GGGAAAGGAG AAATCAAGAA 
ATCCAGCAGG GAGGAGAGGC CTTTCCAGCT AACTCCCCCC TCTTTCCTGA ACCTATTAAA 
GTTGCCAAGG AAGATAAACT CTCAAGTCGC ATCCAGAGCA TGCTGGGAAA TTACGACGAG 
ATGAAGGAAA CCATTGGTGA ACCGCCAATG TCCAAGCTCA TTTCCAAAGC TTCCAGCTCT 
TCCTCCGAGG ATAAATCTGG CCAGTATGGT GACCAGCGCG GAGGCTCTCA GACTCAAAGC 
AGCAAGTGGA CCCCGGTGGG TCCGGCCTCA GGAGGTGGCA ACTCTTCATC GTCTTCTCAG 
AAACGCTCAA CTGTGCAGAG CGTCAGCCAG AAGAGCAGCT CCAGCGGGCA GCGGCACGAG 
CGGGACTACA GCGGCAGCAA GAAATCCAGC AAGCACAGCA ACGAGCACAA GTCCCACTCC 
AGTTCCAGCC CGGCCAAAGT CTCCAGCAAC CACTCGCGCC GAGTCCCCAA GTCCCCCAGA 
GACAGAGAGG CCAATTGGGA CTCGCCCTCC ATCCCGCATT CCTTCCCCAG TGGACATCCC 
AACCAAACCT TCCCACCCTC GCTCATCAAA TCCAGCTCCA TGCCCCAGAA GCCCACCGCT 
TACGTTCGGC CCATGGACGG CCAGGAGACG ACCGAGGCAA AGACGTCTTC AGATTCGTAC 
AGCGGCCAAT CGCACAGCAG CACAGTTGGA GAAATGAAGC CCAATGGAAA GGGCCCACTC 
CCCAAGCTCA AGATCCCAAC ACAGCCTCCA GAGGCGGGGG ATGCGAGCTG TGTTGATGAG 
ATTTTAAAGG AAATGACTCA GGTCTGGCCT CCTCCATTAA CAGCCATTCA CACCCCCTGC 
AAGACCGAGC CGCCTTCCAA ATTCCCCTTC TCCAACGCTA AGGATGCCCA GCACCAGTCT 
GTTGGGGCTC AAAAAAAGTT CTCAGGGAAG AGCGCATCAA CCAGTCACCC GTCAAAGCCA 
TGTGACAGCG AGCAGTCCAA CATGCTTCAT GATGATCTTA AGCTCAGCAG CAGTGAAGAC 
AGTGACGGAG AGCAAGATCC TGCCAAAAAC ACCCCTCAAA ACAACTCCAC AAGTAACAAC 
AGTAATAACA GTGAGGGAGC GGAGCGAGAG GACACCAGCA GTCACAGCGG CTCCGAGAGC 
AGCTCCGGGT CCGACAGCGA GAGCGAGAGC AGCTCCACCG ACAGCGAGAC CACCGAACCG 
CCACGAGCTG CGACCCCCGA GCGGGAACAG CCTACGGCTA ACAAATGGCT TCTGGACAAC 
TGGTTAAATA AGGTCAACAA GCTGTCTCCT GCTTCTCCGG TGGAAAACAG CAGCAGCAGC 
ACGCCGGGAA CCAAGTATAA GAAGGAGGGT CGAGATACCA GCTCCAGCAG GAGTTACAGT 
GGGCAGGGGT CTAAAGAAGC TGCAACGTCA GCCGGCCGAG AGTTGCGACC CAATCAGAAG 
AGCTCCGAAA GCTGGCGAGG ACGCAGTCAG AAATCTCCTG CTCAGAGCGA GGGCACAAGT 
CAATCCGGGC GACGGGTCGT AGGCAAAAAA CAGCCCAAAA AACCTGAGAA GCCGCCGGTG 
GTTGAAGAGC CCAAGGGTGG ACTAAAAGTA GAAACCGAAC CATCGCCGGA AATCCCGTCA 
CACAGAGCTC GCGCTCCCAA TAAAGGCCTG CGCAAACCCA ACATTAAAAA AGAACCCAAA 
ACCTCCTCTC CACGACCTCC GCCTGATAAG CGCAAGACGA AAGCGGCAAA AGTGCCCAGC 
AAGTCTCGCG AGTTCATCGA ATCGGATTCG TCGACGTCGG ATTCTGAAGG CAACGAAAGC 
GACCCATTGC CTTCTCAGAC GCCGAAAGAT TACAGCGAAA ACCCGTTGGT GCCACTCTCG 
CCCATTTATG ACCCGCCCAG ACTGCTGGTG CAGATCAATC TCAGCCTGCT GACTAGAGTC 
CCGGGACAGC TCTACAATGA GACGGAGGTT AAGGTGGAGC GAGGGTCTCC AGCGGAGGGA 
AAAAGCAACC AGAAGCAGCC CGGAGAAAAG ATAAGCAAGG AAGGAAGGAA GAGGAAACAC 
AAGAATGAAG ATGATGGTGT GAAACCAGAG AGTAAGAAGT CCAAACTGGA TGAGAAGTCT 
TCACGCAAAT CAAGCAGTAA TGATTCTTCC AAGCGTGCTC AGGAAAAAGA GAAGGAGTCT 
GCGCCGTCTC CCTCCATCAC ACTGCAGCGG ACGCCCAAAT CTGAGCAGCA GGGGCGCAAG 
CGGACCCTCA GCCAGTCCTC CAGCACCGTC CCCAGCAAAG ACAAGAGCAG CTCCAGCTCC 
AGACACCGCA GAGGAGACGA CAAACCCCAG CGCAGAGAGC CCAAGGAGAA AAGCTCCAAA 
TCAGAGAATC CCGTTTCAGT GCCGCCGCTC TCTGAGGGCT CCAAATCTAG ATCTAAACTT 
CTGTTTGAGG ACAGGGTTCA TTCGGCTGAT CATTACCTTC AAGAAGCCAA GAAGTTGAAA 
CACAATGCGG ATGCTCTGGT GAGGATGGAC AGATTTGAGA AGGCGGTGTA TTACCTGGAC 
GCCGTGGTGT CTTTCATCGA GTGTGGAAAT GCCCTGGAGA AAAACGCCCA GGAGGCCAAA 
TCCCCCTTTC CCATGTATGC AGAAACCGTG GAGCTCATCA AGTACACTAT GAAGCTAAAG 
AGCTACATGG CACCAGATGC AACTTCTCAA GACAAAAGGC TAGCAGTGCT ATGTCTTCGA 
TGTCAAGCGC TCCTGTATTT GAGGCTTTTC AAGCTGCGGA AAGAAAGTGC ACTGAAATAC 
TCTAAAACAC TCACTGAACA CTTGAAGAAT TCATTGAGTA ATACCCAAGC TCCGTCTCCT 
GGAGTAGCAA ATAAAAGCGC AGGGATGCCG TCTCCGGTCT CCCCCAAACT CTCTCCTGGC 
AGTGCGGGCA GCTACTCCTC CAGCAGCTCC AGCCAAAGCG CCAGCTCCTC CGTCACCATC 
CCGCAGCGCA TCCACCAGAT GGCGGCAAGC TACGTGCAAG TCACCTCCAA CTTCCTGTAC 
GCCACCGAGG TGTGGGACCA GGCTGAGCAG CTCGCCAAAG AGCAGAGAGA GTTCTTCACT 
GAGCTGGACA AGGCGATGGG TCCGCTGATC TTCAACACCA GCAGCATGAC TGACCTGGTG 
CGGTTCACGC GGCAGGGCCT GCACTGGCTA CGACTGGACG CCAAGCTCAT CCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_684327.6
CDS	352..3588
Translation	MNREDRNVLRMKERERRNQEIQQGGEAFPANSPLFPEPIKVAKEDKLSSRIQSMLGNYDEMKETIGEPPMSKLISKASSSSSEDKSGQYGDQRGGSQTQSSKWTPVGPASGGGNSSSSSQKRSTVQSVSQKSSSSGQRHERDYSGSKKSSKHSNEHKSHSSSSPAKVSSNHSRRVPKSPRDREANWDSPSIPHSFPSGHPNQTFPPSLIKSSSMPQKPTAYVRPMDGQETTEAKTSSDSYSGQSHSSTVGEMKPNGKGPLPKLKIPTQPPEAGDASCVDEILKEMTQVWPPPLTAIHTPCKTEPPSKFPFSNAKDAQHQSVGAQKKFSGKSASTSHPSKPCDSEQSNMLHDDLKLSSSEDSDGEQDPAKNTPQNNSTSNNSNNSEGAEREDTSSHSGSESSSGSDSESESSSTDSETTEPPRAATPEREQPTANKWLLDNWLNKVNKLSPASPVENSSSSTPGTKYKKEGRDTSSSRSYSGQGSKEAATSAGRELRPNQKSSESWRGRSQKSPAQSEGTSQSGRRVVGKKQPKKPEKPPVVEEPKGGLKVETEPSPEIPSHRARAPNKGLRKPNIKKEPKTSSPRPPPDKRKTKAAKVPSKSREFIESDSSTSDSEGNESDPLPSQTPKDYSENPLVPLSPIYDPPRLLVQINLSLLTRVPGQLYNETEVKVERGSPAEGKSNQKQPGEKISKEGRKRKHKNEDDGVKPESKKSKLDEKSSRKSSSNDSSKRAQEKEKESAPSPSITLQRTPKSEQQGRKRTLSQSSSTVPSKDKSSSSSRHRRGDDKPQRREPKEKSSKSENPVSVPPLSEGSKSRSKLLFEDRVHSADHYLQEAKKLKHNADALVRMDRFEKAVYYLDAVVSFIECGNALEKNAQEAKSPFPMYAETVELIKYTMKLKSYMAPDATSQDKRLAVLCLRCQALLYLRLFKLRKESALKYSKTLTEHLKNSLSNTQAPSPGVANKSAGMPSPVSPKLSPGSAGSYSSSSSSQSASSSVTIPQRIHQMAASYVQVTSNFLYATEVWDQAEQLAKEQREFFTELDKAMGPLIFNTSSMTDLVRFTRQGLHWLRLDAKLIQ

Target ORF information:

RefSeq Version	XM_679235.9
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio AF4/FMR2 family, member 4 (aff4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_679235.9

ORF Insert Sequence:

ATGAACCGTG AAGACCGGAA TGTGCTCCGT ATGAAAGAAA GGGAAAGGAG AAATCAAGAA 
ATCCAGCAGG GAGGAGAGGC CTTTCCAGCT AACTCCCCCC TCTTTCCTGA ACCTATTAAA 
GTTGCCAAGG AAGATAAACT CTCAAGTCGC ATCCAGAGCA TGCTGGGAAA TTACGACGAG 
ATGAAGGAAA CCATTGGTGA ACCGCCAATG TCCAAGCTCA TTTCCAAAGC TTCCAGCTCT 
TCCTCCGAGG ATAAATCTGG CCAGTATGGT GACCAGCGCG GAGGCTCTCA GACTCAAAGC 
AGCAAGTGGA CCCCGGTGGG TCCGGCCTCA GGAGGTGGCA ACTCTTCATC GTCTTCTCAG 
AAACGCTCAA CTGTGCAGAG CGTCAGCCAG AAGAGCAGCT CCAGCGGGCA GCGGCACGAG 
CGGGACTACA GCGGCAGCAA GAAATCCAGC AAGCACAGCA ACGAGCACAA GTCCCACTCC 
AGTTCCAGCC CGGCCAAAGT CTCCAGCAAC CACTCGCGCC GAGTCCCCAA GTCCCCCAGA 
GACAGAGAGG CCAATTGGGA CTCGCCCTCC ATCCCGCATT CCTTCCCCAG TGGACATCCC 
AACCAAACCT TCCCACCCTC GCTCATCAAA TCCAGCTCCA TGCCCCAGAA GCCCACCGCT 
TACGTTCGGC CCATGGACGG CCAGGAGACG ACCGAGGCAA AGACGTCTTC AGATTCGTAC 
AGCGGCCAAT CGCACAGCAG CACAGTTGGA GAAATGAAGC CCAATGGAAA GGGCCCACTC 
CCCAAGCTCA AGATCCCAAC ACAGCCTCCA GAGGCGGGGG ATGCGAGCTG TGTTGATGAG 
ATTTTAAAGG AAATGACTCA GGTCTGGCCT CCTCCATTAA CAGCCATTCA CACCCCCTGC 
AAGACCGAGC CGCCTTCCAA ATTCCCCTTC TCCAACGCTA AGGATGCCCA GCACCAGTCT 
GTTGGGGCTC AAAAAAAGTT CTCAGGGAAG AGCGCATCAA CCAGTCACCC GTCAAAGCCA 
TGTGACAGCG AGCAGTCCAA CATGCTTCAT GATGATCTTA AGCTCAGCAG CAGTGAAGAC 
AGTGACGGAG AGCAAGATCC TGCCAAAAAC ACCCCTCAAA ACAACTCCAC AAGTAACAAC 
AGTAATAACA GTGAGGGAGC GGAGCGAGAG GACACCAGCA GTCACAGCGG CTCCGAGAGC 
AGCTCCGGGT CCGACAGCGA GAGCGAGAGC AGCTCCACCG ACAGCGAGAC CACCGAACCG 
CCACGAGCTG CGACCCCCGA GCGGGAACAG CCTACGGCTA ACAAATGGCT TCTGGACAAC 
TGGTTAAATA AGGTCAACAA GCTGTCTCCT GCTTCTCCGG TGGAAAACAG CAGCAGCAGC 
ACGCCGGGAA CCAAGTATAA GAAGGAGGGT CGAGATACCA GCTCCAGCAG GAGTTACAGT 
GGGCAGGGGT CTAAAGAAGC TGCAACGTCA GCCGGCCGAG AGTTGCGACC CAATCAGAAG 
AGCTCCGAAA GCTGGCGAGG ACGCAGTCAG AAATCTCCTG CTCAGAGCGA GGGCACAAGT 
CAATCCGGGC GACGGGTCGT AGGCAAAAAA CAGCCCAAAA AACCTGAGAA GCCGCCGGTG 
GTTGAAGAGC CCAAGGGTGG ACTAAAAGTA GAAACCGAAC CATCGCCGGA AATCCCGTCA 
CACAGAGCTC GCGCTCCCAA TAAAGGCCTG CGCAAACCCA ACATTAAAAA AGAACCCAAA 
ACCTCCTCTC CACGACCTCC GCCTGATAAG CGCAAGACGA AAGCGGCAAA AGTGCCCAGC 
AAGTCTCGCG AGTTCATCGA ATCGGATTCG TCGACGTCGG ATTCTGAAGG CAACGAAAGC 
GACCCATTGC CTTCTCAGAC GCCGAAAGAT TACAGCGAAA ACCCGTTGGT GCCACTCTCG 
CCCATTTATG ACCCGCCCAG ACTGCTGGTG CAGATCAATC TCAGCCTGCT GACTAGAGTC 
CCGGGACAGC TCTACAATGA GACGGAGGTT AAGGTGGAGC GAGGGTCTCC AGCGGAGGGA 
AAAAGCAACC AGAAGCAGCC CGGAGAAAAG ATAAGCAAGG AAGGAAGGAA GAGGAAACAC 
AAGAATGAAG ATGATGGTGT GAAACCAGAG AGTAAGAAGT CCAAACTGGA TGAGAAGTCT 
TCACGCAAAT CAAGCAGTAA TGATTCTTCC AAGCGTGCTC AGGAAAAAGA GAAGGAGTCT 
GCGCCGTCTC CCTCCATCAC ACTGCAGCGG ACGCCCAAAT CTGAGCAGCA GGGGCGCAAG 
CGGACCCTCA GCCAGTCCTC CAGCACCGTC CCCAGCAAAG ACAAGAGCAG CTCCAGCTCC 
AGACACCGCA GAGGAGACGA CAAACCCCAG CGCAGAGAGC CCAAGGAGAA AAGCTCCAAA 
TCAGAGAATC CCGTTTCAGT GCCGCCGCTC TCTGAGGGCT CCAAATCTAG ATCTAAACTT 
CTGTTTGAGG ACAGGGTTCA TTCGGCTGAT CATTACCTTC AAGAAGCCAA GAAGTTGAAA 
CACAATGCGG ATGCTCTGGT GAGGATGGAC AGATTTGAGA AGGCGGTGTA TTACCTGGAC 
GCCGTGGTGT CTTTCATCGA GTGTGGAAAT GCCCTGGAGA AAAACGCCCA GGAGGCCAAA 
TCCCCCTTTC CCATGTATGC AGAAACCGTG GAGCTCATCA AGTACACTAT GAAGCTAAAG 
AGCTACATGG CACCAGATGC AACTTCTCAA GACAAAAGGC TAGCAGTGCT ATGTCTTCGA 
TGTCAAGCGC TCCTGTATTT GAGGCTTTTC AAGCTGCGGA AAGAAAGTGC ACTGAAATAC 
TCTAAAACAC TCACTGAACA CTTGAAGAAT TCATTGAGTA ATACCCAAGC TCCGTCTCCT 
GGAGTAGCAA ATAAAAGCGC AGGGATGCCG TCTCCGGTCT CCCCCAAACT CTCTCCTGGC 
AGTGCGGGCA GCTACTCCTC CAGCAGCTCC AGCCAAAGCG CCAGCTCCTC CGTCACCATC 
CCGCAGCGCA TCCACCAGAT GGCGGCAAGC TACGTGCAAG TCACCTCCAA CTTCCTGTAC 
GCCACCGAGG TGTGGGACCA GGCTGAGCAG CTCGCCAAAG AGCAGAGAGA GTTCTTCACT 
GAGCTGGACA AGGCGATGGG TCCGCTGATC TTCAACACCA GCAGCATGAC TGACCTGGTG 
CGGTTCACGC GGCAGGGCCT GCACTGGCTA CGACTGGACG CCAAGCTCAT CCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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AFF4 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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