prickle1b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	prickle1b
Entrez Gene ID	556148
Full Name	prickle homolog 1b
Synonyms	fb98c11,si:ch211-155a11.1,wu:fb98c11
General protein information	Preferred Names prickle homolog 1b Names prickle-like protein 1 pk1b prickle homolog 1 b
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005164634.4	XP_005164691.1	prickle-like protein 1 isoform X1
XM_005164634.3	XP_005164691.1	prickle-like protein 1 isoform X1
NM_001030098.2	NP_001025269.1	prickle-like protein 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Prickle1 is required for EMT and migration of zebrafish cranial neural crest.
Ahsan K, Singh N, Rocha M, Huang C, Prince VE
Developmental biology448(1)16-35(2019 04)

Planar cell polarity genes Frizzled3a, Vangl2, and Scribble are required for spinal commissural axon guidance.
Sun SD, Purdy AM, Walsh GS
BMC neuroscience17(1)83(2016 12)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

CDX4 and retinoic acid interact to position the hindbrain-spinal cord transition.
Chang J, Skromne I, Ho RK
Developmental biology410(2)178-189(2016 Feb)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following prickle1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the prickle1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa02233	NM_001030098.2 Latest version!	Danio rerio prickle homolog 1b (prickle1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa09250	XM_005164634.4 Latest version!	Danio rerio prickle homolog 1b (prickle1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
ODa09250	XM_005164634.3	Danio rerio prickle homolog 1b (prickle1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

prickle1b ( NM_001030098.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001025269.1 Danio rerio prickle homolog 1b (prickle1b), mRNA.

Price & Availability↑

CloneID	ODa02233
Clone ID Related Accession (Same CDS sequence)	NM_001030098.2
Accession Version	NM_001030098.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1572019200000
Organism	Danio rerio(zebrafish)
Product	prickle-like protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EB928759.1, AL929504.9 and AI588252.1. On May 1, 2009 this sequence version replaced NM_001030098.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCATTACG CAAAGTGCCC CCATCCCATG AGTCTTGAGA CTACATCTGG GATCTGGCCC 
ATTACTCGGG CACCGGACAT GGACTCGAGA GGAGCCAAGG CATTTGGGTT TCAGAGGAGT 
TCAACGTCTG ATGACGACTC TGGATGTGCT CTTGAGGAGT ACACATGGGT TCCTCCTGGA 
CTACGGGCTG AGCAGGTTCA GATCTACTTT TCGTGCCTAC CAGAAGAGAA GGTGCCATAT 
GTCAACAGCC CTGGAGAGAA GCACAGGATC AGACAACTAC TCTATCAGCT GCCACCCCAC 
GACAATGAGA TTCGATACTG CCAGTCCCTC AGTGATGAAG AGAGACGAGA ACTCCACATG 
TTCAGCATGC AAAGAAAGAA AGAGGCACTT GGGAGGGGGA CCCCAAAACT GCTTCCCAGA 
GCTCTGCAAC ATAATTGTGA GCATTGTAAA GAGAACATCA ACGGTGGAGA GATGGCAGTG 
TTTGCTTCGA GAGCAGGCCC CGGCCCTTGC TGGCACCCAG CATGCTTTAC TTGCTACACC 
TGTCATGAAC TTCTGGTGGA CCTCATCTAT TTCTACCACA ATGGCAATAT CCACTGCGGC 
AGACATCATG CAGAGCTTCT GAAGCCACGT TGCTCAGCAT GTGATGAGAT TATCTTTGCT 
GACGAGTGTA CCGAAGCTGA GGGTCGTCAC TGGCACATGA AACATTTTTC ATGCTTTGAG 
TGCGAGACTA TTTTAGGAGG ACAGAGGTAC ATCATGAAAG ATGGCCGACC CTTCTGTTGT 
GGATGCTTTG AGTCATTGTA TGCAGAGTAC TGCGAGGCCT GTGGAGAGAA TATTGGAGTC 
GACCATGCTC AAATGACGTA CGAGGGTGTA CATTGGCACG CTACTGACAA ATGCTTTTGT 
TGTGCACAAT GTAAAACCTC TCTGCTGGGC TGTCCCTTCC TTCCAAAAGA TGGTCGTATC 
TACTGCTCTA AGGACTGTAG CCTAGGTGAG GATGTCCAGG CTTCTGATTC TTCAGACTCC 
GCTTTTCAGT CCGCCAGATC ACGGGAATCT CGACGCAGTA TTCGAATGGG CAAGAGTAGT 
CGCCCCACAG AACAGTGCCA GCAGACAAAA TTGTACTCAG CAACAGATTA CAGGACCTCT 
AGTCTATGTG GCGACAACAC AGAGACAATG TGTCATCAGC TGGAGAGAAT CAACTTTACA 
GAAGACAACT ACTGGAGAGA GCGAGATGAG CAGGAAGCCC CTGAGGACTC TGTGGAAGAT 
TGGGCTGAGC ATGAGGACTA CATGACTCAG CTGCTCCTGA AATTTGGAGA CCGTGGTGTC 
ATCCACGCAG AGGACAGCAG GGCAGCAGAG CCATGGATGG TGAAAGACAC CGAAACCAAC 
CGAACCAAAC CAGAGATAAA GATGAGCGGC CACAATTTGG CAAGTAAAAA GCACCACACT 
GACATGTATT GGGCCCAGTC TCAGGATGGA CTTGGAGACT CTGCTTATGG TAGTCACCCT 
GGACCAGCTA GCGCTAGAAA GATTCAAGAG CTGGAGATGG ATCAGGGAAC AGTACCTGGA 
TTTTGGTCTG AGCCAAGGCA ATGGTATGAA GACTCATTAG AGTGCATTAC AAGTGAACTC 
CGGAAGGCAG ACCACAATAT TGGAGATTCT ATGGATTCTC TGGCTCTCTC AAATATTACA 
GGAGCTTCAG TGGATGGGGA CAGCAAGGAC AGACAGACCA TTTACTCCCT CCAAAACATG 
AAAGACCAAG AAAGAGAGAT AGAAGAGTGT GATAACATTA GTAACGTAGG CACACTAAAC 
TCCTCAATGC TCCACAGAAG TGCTAATTCC CTGAGGAGTT TAAACTCCGA GCTAGGTCAT 
GAGGAGACAC TGGGGCAGGG AGCAAGAGAA GACGATGAAG AGGACAGAAT ACCACCATAT 
TTTGGAGCAG AACCACCATC TCCTCTGAAT CTTCCAGTGC TGAGAAGGAC CAAGTCACAA 
TCTCAGCCGC AATCCAGATC TCAGCAAGTA AAGTTCTCTG ATGATGTTCT GGAGAAAGGG 
TATTATGGTG CACTGGGCTT AAGGCAACCA CCAATGAGTG AGAGGACACG CAGGAGGACC 
TTTCACATAG AGGAGGAGGC AGCGGCAGCA GCAGAGAGAC GTCCTGCTAA CAGACATCAC 
CACAAACGCA GGCACAGTCG GAAAACGCGT TCAGAAAATG CATTGCACCT GCCTTCCAAA 
AATCGTGGGA AACTGCATGT TGTGCCTCAG GTAATCAGCT CAAACATGCC AGAGCCCAGG 
AATGGTATTC TACTAAATCA CTCGGCGCAC AGTCAGCACC CTTATCGTCA AACCACCTCA 
GACTTTCCTC AGCAAGGTAT GGTCCGACTG GACCAATTCC AGGACTTTTG TGATGATGAC 
GAAGAAGATG ATTGGTGTTC AACATGCTCT TCTTCATCTT CAGAATCTGA GGAGGAGGGA 
TTCTTTCTAG GTCAGCCAAT CCCTAAGCCC CGATCCCAAA GTTTTCATTA TTACACAGAG 
GACTATCCGA CTCGGGTCAC GGCTTTAACG CCTCCTTTTG GCTCAAGGAC CAAACTGAAA 
AAGAAAGGTC ACAAGGGCAA GAACTGCATA ATTTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001025269.1
CDS	128..2746
Translation	MHYAKCPHPMSLETTSGIWPITRAPDMDSRGAKAFGFQRSSTSDDDSGCALEEYTWVPPGLRAEQVQIYFSCLPEEKVPYVNSPGEKHRIRQLLYQLPPHDNEIRYCQSLSDEERRELHMFSMQRKKEALGRGTPKLLPRALQHNCEHCKENINGGEMAVFASRAGPGPCWHPACFTCYTCHELLVDLIYFYHNGNIHCGRHHAELLKPRCSACDEIIFADECTEAEGRHWHMKHFSCFECETILGGQRYIMKDGRPFCCGCFESLYAEYCEACGENIGVDHAQMTYEGVHWHATDKCFCCAQCKTSLLGCPFLPKDGRIYCSKDCSLGEDVQASDSSDSAFQSARSRESRRSIRMGKSSRPTEQCQQTKLYSATDYRTSSLCGDNTETMCHQLERINFTEDNYWRERDEQEAPEDSVEDWAEHEDYMTQLLLKFGDRGVIHAEDSRAAEPWMVKDTETNRTKPEIKMSGHNLASKKHHTDMYWAQSQDGLGDSAYGSHPGPASARKIQELEMDQGTVPGFWSEPRQWYEDSLECITSELRKADHNIGDSMDSLALSNITGASVDGDSKDRQTIYSLQNMKDQEREIEECDNISNVGTLNSSMLHRSANSLRSLNSELGHEETLGQGAREDDEEDRIPPYFGAEPPSPLNLPVLRRTKSQSQPQSRSQQVKFSDDVLEKGYYGALGLRQPPMSERTRRRTFHIEEEAAAAAERRPANRHHHKRRHSRKTRSENALHLPSKNRGKLHVVPQVISSNMPEPRNGILLNHSAHSQHPYRQTTSDFPQQGMVRLDQFQDFCDDDEEDDWCSTCSSSSSESEEEGFFLGQPIPKPRSQSFHYYTEDYPTRVTALTPPFGSRTKLKKKGHKGKNCIIS

Target ORF information:

RefSeq Version	NM_001030098.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio prickle homolog 1b (prickle1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001030098.2

ORF Insert Sequence:

ATGCATTACG CAAAGTGCCC CCATCCCATG AGTCTTGAGA CTACATCTGG GATCTGGCCC 
ATTACTCGGG CACCGGACAT GGACTCGAGA GGAGCCAAGG CATTTGGGTT TCAGAGGAGT 
TCAACGTCTG ATGACGACTC TGGATGTGCT CTTGAGGAGT ACACATGGGT TCCTCCTGGA 
CTACGGGCTG AGCAGGTTCA GATCTACTTT TCGTGCCTAC CAGAAGAGAA GGTGCCATAT 
GTCAACAGCC CTGGAGAGAA GCACAGGATC AGACAACTAC TCTATCAGCT GCCACCCCAC 
GACAATGAGA TTCGATACTG CCAGTCCCTC AGTGATGAAG AGAGACGAGA ACTCCACATG 
TTCAGCATGC AAAGAAAGAA AGAGGCACTT GGGAGGGGGA CCCCAAAACT GCTTCCCAGA 
GCTCTGCAAC ATAATTGTGA GCATTGTAAA GAGAACATCA ACGGTGGAGA GATGGCAGTG 
TTTGCTTCGA GAGCAGGCCC CGGCCCTTGC TGGCACCCAG CATGCTTTAC TTGCTACACC 
TGTCATGAAC TTCTGGTGGA CCTCATCTAT TTCTACCACA ATGGCAATAT CCACTGCGGC 
AGACATCATG CAGAGCTTCT GAAGCCACGT TGCTCAGCAT GTGATGAGAT TATCTTTGCT 
GACGAGTGTA CCGAAGCTGA GGGTCGTCAC TGGCACATGA AACATTTTTC ATGCTTTGAG 
TGCGAGACTA TTTTAGGAGG ACAGAGGTAC ATCATGAAAG ATGGCCGACC CTTCTGTTGT 
GGATGCTTTG AGTCATTGTA TGCAGAGTAC TGCGAGGCCT GTGGAGAGAA TATTGGAGTC 
GACCATGCTC AAATGACGTA CGAGGGTGTA CATTGGCACG CTACTGACAA ATGCTTTTGT 
TGTGCACAAT GTAAAACCTC TCTGCTGGGC TGTCCCTTCC TTCCAAAAGA TGGTCGTATC 
TACTGCTCTA AGGACTGTAG CCTAGGTGAG GATGTCCAGG CTTCTGATTC TTCAGACTCC 
GCTTTTCAGT CCGCCAGATC ACGGGAATCT CGACGCAGTA TTCGAATGGG CAAGAGTAGT 
CGCCCCACAG AACAGTGCCA GCAGACAAAA TTGTACTCAG CAACAGATTA CAGGACCTCT 
AGTCTATGTG GCGACAACAC AGAGACAATG TGTCATCAGC TGGAGAGAAT CAACTTTACA 
GAAGACAACT ACTGGAGAGA GCGAGATGAG CAGGAAGCCC CTGAGGACTC TGTGGAAGAT 
TGGGCTGAGC ATGAGGACTA CATGACTCAG CTGCTCCTGA AATTTGGAGA CCGTGGTGTC 
ATCCACGCAG AGGACAGCAG GGCAGCAGAG CCATGGATGG TGAAAGACAC CGAAACCAAC 
CGAACCAAAC CAGAGATAAA GATGAGCGGC CACAATTTGG CAAGTAAAAA GCACCACACT 
GACATGTATT GGGCCCAGTC TCAGGATGGA CTTGGAGACT CTGCTTATGG TAGTCACCCT 
GGACCAGCTA GCGCTAGAAA GATTCAAGAG CTGGAGATGG ATCAGGGAAC AGTACCTGGA 
TTTTGGTCTG AGCCAAGGCA ATGGTATGAA GACTCATTAG AGTGCATTAC AAGTGAACTC 
CGGAAGGCAG ACCACAATAT TGGAGATTCT ATGGATTCTC TGGCTCTCTC AAATATTACA 
GGAGCTTCAG TGGATGGGGA CAGCAAGGAC AGACAGACCA TTTACTCCCT CCAAAACATG 
AAAGACCAAG AAAGAGAGAT AGAAGAGTGT GATAACATTA GTAACGTAGG CACACTAAAC 
TCCTCAATGC TCCACAGAAG TGCTAATTCC CTGAGGAGTT TAAACTCCGA GCTAGGTCAT 
GAGGAGACAC TGGGGCAGGG AGCAAGAGAA GACGATGAAG AGGACAGAAT ACCACCATAT 
TTTGGAGCAG AACCACCATC TCCTCTGAAT CTTCCAGTGC TGAGAAGGAC CAAGTCACAA 
TCTCAGCCGC AATCCAGATC TCAGCAAGTA AAGTTCTCTG ATGATGTTCT GGAGAAAGGG 
TATTATGGTG CACTGGGCTT AAGGCAACCA CCAATGAGTG AGAGGACACG CAGGAGGACC 
TTTCACATAG AGGAGGAGGC AGCGGCAGCA GCAGAGAGAC GTCCTGCTAA CAGACATCAC 
CACAAACGCA GGCACAGTCG GAAAACGCGT TCAGAAAATG CATTGCACCT GCCTTCCAAA 
AATCGTGGGA AACTGCATGT TGTGCCTCAG GTAATCAGCT CAAACATGCC AGAGCCCAGG 
AATGGTATTC TACTAAATCA CTCGGCGCAC AGTCAGCACC CTTATCGTCA AACCACCTCA 
GACTTTCCTC AGCAAGGTAT GGTCCGACTG GACCAATTCC AGGACTTTTG TGATGATGAC 
GAAGAAGATG ATTGGTGTTC AACATGCTCT TCTTCATCTT CAGAATCTGA GGAGGAGGGA 
TTCTTTCTAG GTCAGCCAAT CCCTAAGCCC CGATCCCAAA GTTTTCATTA TTACACAGAG 
GACTATCCGA CTCGGGTCAC GGCTTTAACG CCTCCTTTTG GCTCAAGGAC CAAACTGAAA 
AAGAAAGGTC ACAAGGGCAA GAACTGCATA ATTTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prickle1b ( XM_005164634.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164691.1 Danio rerio prickle homolog 1b (prickle1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa09250
Clone ID Related Accession (Same CDS sequence)	XM_005164634.4 , XM_005164634.3
Accession Version	XM_005164634.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2592bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	prickle-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005164634.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCTTG AGACTACATC TGGGATCTGG CCCATTACTC GGGCACCGGA CATGGACTCG 
AGAGGAGCCA AGGCATTTGG GTTTCAGAGG AGTTCAACGT CTGATGACGA CTCTGGATGT 
GCTCTTGAGG AGTACACATG GGTTCCTCCT GGACTACGGG CTGAGCAGGT TCAGATCTAC 
TTTTCGTGCC TACCAGAAGA GAAGGTGCCA TATGTCAACA GCCCTGGAGA GAAGCACAGG 
ATCAGACAAC TACTCTATCA GCTGCCACCC CACGACAATG AGATTCGATA CTGCCAGTCC 
CTCAGTGATG AAGAGAGACG AGAACTCCAC ATGTTCAGCA TGCAAAGAAA GAAAGAGGCA 
CTTGGGAGGG GGACCCCAAA ACTGCTTCCC AGAGCTCTGC AACATAATTG TGAGCATTGT 
AAAGAGAACA TCAACGGTGG AGAGATGGCA GTGTTTGCTT CGAGAGCAGG CCCCGGCCCT 
TGCTGGCACC CAGCATGCTT TACTTGCTAC ACCTGTCATG AACTTCTGGT GGACCTCATC 
TATTTCTACC ACAATGGCAA TATCCACTGC GGCAGACATC ATGCAGAGCT TCTGAAGCCA 
CGTTGCTCAG CATGTGATGA GATTATCTTT GCTGACGAGT GTACCGAAGC TGAGGGTCGT 
CACTGGCACA TGAAACATTT TTCATGCTTT GAGTGCGAGA CTATTTTAGG AGGACAGAGG 
TACATCATGA AAGATGGCCG ACCCTTCTGT TGTGGATGCT TTGAGTCATT GTATGCAGAG 
TACTGCGAGG CCTGTGGAGA GAATATTGGA GTCGACCATG CTCAAATGAC GTACGAGGGT 
GTACATTGGC ACGCTACTGA CAAATGCTTT TGTTGTGCAC AATGTAAAAC CTCTCTGCTG 
GGCTGTCCCT TCCTTCCAAA AGATGGTCGT ATCTACTGCT CTAAGGACTG TAGCCTAGGT 
GAGGATGTCC AGGCTTCTGA TTCTTCAGAC TCCGCTTTTC AGTCCGCCAG ATCACGGGAA 
TCTCGACGCA GTATTCGAAT GGGCAAGAGT AGTCGCCCCA CAGAACAGTG CCAGCAGACA 
AAATTGTACT CAGCAACAGA TTACAGGACC TCTAGTCTAT GTGGCGACAA CACAGAGACA 
ATGTGTCATC AGCTGGAGAG AATCAACTTT ACAGAAGACA ACTACTGGAG AGAGCGAGAT 
GAGCAGGAAG CCCCTGAGGA CTCTGTGGAA GATTGGGCTG AGCATGAGGA CTACATGACT 
CAGCTGCTCC TGAAATTTGG AGACCGTGGT GTCATCCACG CAGAGGACAG CAGGGCAGCA 
GAGCCATGGA TGGTGAAAGA CACCGAAACC AACCGAACCA AACCAGAGAT AAAGATGAGC 
GGCCACAATT TGGCAAGTAA AAAGCACCAC ACTGACATGT ATTGGGCCCA GTCTCAGGAT 
GGACTTGGAG ACTCTGCTTA TGGTAGTCAC CCTGGACCAG CTAGCGCTAG AAAGATTCAA 
GAGCTGGAGA TGGATCAGGG AACAGTACCT GGATTTTGGT CTGAGCCAAG GCAATGGTAT 
GAAGACTCAT TAGAGTGCAT TACAAGTGAA CTCCGGAAGG CAGACCACAA TATTGGAGAT 
TCTATGGATT CTCTGGCTCT CTCAAATATT ACAGGAGCTT CAGTGGATGG GGACAGCAAG 
GACAGACAGA CCATTTACTC CCTCCAAAAC ATGAAAGACC AAGAAAGAGA GATAGAAGAG 
TGTGATAACA TTAGTAACGT AGGCACACTA AACTCCTCAA TGCTCCACAG AAGTGCTAAT 
TCCCTGAGGA GTTTAAACTC CGAGCTAGGT CATGAGGAGA CACTGGGGCA GGGAGCAAGA 
GAAGACGATG AAGAGGACAG AATACCACCA TATTTTGGAG CAGAACCACC ATCTCCTCTG 
AATCTTCCAG TGCTGAGAAG GACCAAGTCA CAATCTCAGC CGCAATCCAG ATCTCAGCAA 
GTAAAGTTCT CTGATGATGT TCTGGAGAAA GGGTATTATG GTGCACTGGG CTTAAGGCAA 
CCACCAATGA GTGAGAGGAC ACGCAGGAGG ACCTTTCACA TAGAGGAGGA GGCAGCGGCA 
GCAGCAGAGA GACGTCCTGC TAACAGACAT CACCACAAAC GCAGGCACAG TCGGAAAACG 
CGTTCAGAAA ATGCATTGCA CCTGCCTTCC AAAAATCGTG GGAAACTGCA TGTTGTGCCT 
CAGGTAATCA GCTCAAACAT GCCAGAGCCC AGGAATGGTA TTCTACTAAA TCACTCGGCG 
CACAGTCAGC ACCCTTATCG TCAAACCACC TCAGACTTTC CTCAGCAAGG TATGGTCCGA 
CTGGACCAAT TCCAGGACTT TTGTGATGAT GACGAAGAAG ATGATTGGTG TTCAACATGC 
TCTTCTTCAT CTTCAGAATC TGAGGAGGAG GGATTCTTTC TAGGTCAGCC AATCCCTAAG 
CCCCGATCCC AAAGTTTTCA TTATTACACA GAGGACTATC CGACTCGGGT CACGGCTTTA 
ACGCCTCCTT TTGGCTCAAG GACCAAACTG AAAAAGAAAG GTCACAAGGG CAAGAACTGC 
ATAATTTCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164691.1
CDS	421..3012
Translation	MSLETTSGIWPITRAPDMDSRGAKAFGFQRSSTSDDDSGCALEEYTWVPPGLRAEQVQIYFSCLPEEKVPYVNSPGEKHRIRQLLYQLPPHDNEIRYCQSLSDEERRELHMFSMQRKKEALGRGTPKLLPRALQHNCEHCKENINGGEMAVFASRAGPGPCWHPACFTCYTCHELLVDLIYFYHNGNIHCGRHHAELLKPRCSACDEIIFADECTEAEGRHWHMKHFSCFECETILGGQRYIMKDGRPFCCGCFESLYAEYCEACGENIGVDHAQMTYEGVHWHATDKCFCCAQCKTSLLGCPFLPKDGRIYCSKDCSLGEDVQASDSSDSAFQSARSRESRRSIRMGKSSRPTEQCQQTKLYSATDYRTSSLCGDNTETMCHQLERINFTEDNYWRERDEQEAPEDSVEDWAEHEDYMTQLLLKFGDRGVIHAEDSRAAEPWMVKDTETNRTKPEIKMSGHNLASKKHHTDMYWAQSQDGLGDSAYGSHPGPASARKIQELEMDQGTVPGFWSEPRQWYEDSLECITSELRKADHNIGDSMDSLALSNITGASVDGDSKDRQTIYSLQNMKDQEREIEECDNISNVGTLNSSMLHRSANSLRSLNSELGHEETLGQGAREDDEEDRIPPYFGAEPPSPLNLPVLRRTKSQSQPQSRSQQVKFSDDVLEKGYYGALGLRQPPMSERTRRRTFHIEEEAAAAAERRPANRHHHKRRHSRKTRSENALHLPSKNRGKLHVVPQVISSNMPEPRNGILLNHSAHSQHPYRQTTSDFPQQGMVRLDQFQDFCDDDEEDDWCSTCSSSSSESEEEGFFLGQPIPKPRSQSFHYYTEDYPTRVTALTPPFGSRTKLKKKGHKGKNCIIS

Target ORF information:

RefSeq Version	XM_005164634.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio prickle homolog 1b (prickle1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164634.4

ORF Insert Sequence:

ATGAGTCTTG AGACTACATC TGGGATCTGG CCCATTACTC GGGCACCGGA CATGGACTCG 
AGAGGAGCCA AGGCATTTGG GTTTCAGAGG AGTTCAACGT CTGATGACGA CTCTGGATGT 
GCTCTTGAGG AGTACACATG GGTTCCTCCT GGACTACGGG CTGAGCAGGT TCAGATCTAC 
TTTTCGTGCC TACCAGAAGA GAAGGTGCCA TATGTCAACA GCCCTGGAGA GAAGCACAGG 
ATCAGACAAC TACTCTATCA GCTGCCACCC CACGACAATG AGATTCGATA CTGCCAGTCC 
CTCAGTGATG AAGAGAGACG AGAACTCCAC ATGTTCAGCA TGCAAAGAAA GAAAGAGGCA 
CTTGGGAGGG GGACCCCAAA ACTGCTTCCC AGAGCTCTGC AACATAATTG TGAGCATTGT 
AAAGAGAACA TCAACGGTGG AGAGATGGCA GTGTTTGCTT CGAGAGCAGG CCCCGGCCCT 
TGCTGGCACC CAGCATGCTT TACTTGCTAC ACCTGTCATG AACTTCTGGT GGACCTCATC 
TATTTCTACC ACAATGGCAA TATCCACTGC GGCAGACATC ATGCAGAGCT TCTGAAGCCA 
CGTTGCTCAG CATGTGATGA GATTATCTTT GCTGACGAGT GTACCGAAGC TGAGGGTCGT 
CACTGGCACA TGAAACATTT TTCATGCTTT GAGTGCGAGA CTATTTTAGG AGGACAGAGG 
TACATCATGA AAGATGGCCG ACCCTTCTGT TGTGGATGCT TTGAGTCATT GTATGCAGAG 
TACTGCGAGG CCTGTGGAGA GAATATTGGA GTCGACCATG CTCAAATGAC GTACGAGGGT 
GTACATTGGC ACGCTACTGA CAAATGCTTT TGTTGTGCAC AATGTAAAAC CTCTCTGCTG 
GGCTGTCCCT TCCTTCCAAA AGATGGTCGT ATCTACTGCT CTAAGGACTG TAGCCTAGGT 
GAGGATGTCC AGGCTTCTGA TTCTTCAGAC TCCGCTTTTC AGTCCGCCAG ATCACGGGAA 
TCTCGACGCA GTATTCGAAT GGGCAAGAGT AGTCGCCCCA CAGAACAGTG CCAGCAGACA 
AAATTGTACT CAGCAACAGA TTACAGGACC TCTAGTCTAT GTGGCGACAA CACAGAGACA 
ATGTGTCATC AGCTGGAGAG AATCAACTTT ACAGAAGACA ACTACTGGAG AGAGCGAGAT 
GAGCAGGAAG CCCCTGAGGA CTCTGTGGAA GATTGGGCTG AGCATGAGGA CTACATGACT 
CAGCTGCTCC TGAAATTTGG AGACCGTGGT GTCATCCACG CAGAGGACAG CAGGGCAGCA 
GAGCCATGGA TGGTGAAAGA CACCGAAACC AACCGAACCA AACCAGAGAT AAAGATGAGC 
GGCCACAATT TGGCAAGTAA AAAGCACCAC ACTGACATGT ATTGGGCCCA GTCTCAGGAT 
GGACTTGGAG ACTCTGCTTA TGGTAGTCAC CCTGGACCAG CTAGCGCTAG AAAGATTCAA 
GAGCTGGAGA TGGATCAGGG AACAGTACCT GGATTTTGGT CTGAGCCAAG GCAATGGTAT 
GAAGACTCAT TAGAGTGCAT TACAAGTGAA CTCCGGAAGG CAGACCACAA TATTGGAGAT 
TCTATGGATT CTCTGGCTCT CTCAAATATT ACAGGAGCTT CAGTGGATGG GGACAGCAAG 
GACAGACAGA CCATTTACTC CCTCCAAAAC ATGAAAGACC AAGAAAGAGA GATAGAAGAG 
TGTGATAACA TTAGTAACGT AGGCACACTA AACTCCTCAA TGCTCCACAG AAGTGCTAAT 
TCCCTGAGGA GTTTAAACTC CGAGCTAGGT CATGAGGAGA CACTGGGGCA GGGAGCAAGA 
GAAGACGATG AAGAGGACAG AATACCACCA TATTTTGGAG CAGAACCACC ATCTCCTCTG 
AATCTTCCAG TGCTGAGAAG GACCAAGTCA CAATCTCAGC CGCAATCCAG ATCTCAGCAA 
GTAAAGTTCT CTGATGATGT TCTGGAGAAA GGGTATTATG GTGCACTGGG CTTAAGGCAA 
CCACCAATGA GTGAGAGGAC ACGCAGGAGG ACCTTTCACA TAGAGGAGGA GGCAGCGGCA 
GCAGCAGAGA GACGTCCTGC TAACAGACAT CACCACAAAC GCAGGCACAG TCGGAAAACG 
CGTTCAGAAA ATGCATTGCA CCTGCCTTCC AAAAATCGTG GGAAACTGCA TGTTGTGCCT 
CAGGTAATCA GCTCAAACAT GCCAGAGCCC AGGAATGGTA TTCTACTAAA TCACTCGGCG 
CACAGTCAGC ACCCTTATCG TCAAACCACC TCAGACTTTC CTCAGCAAGG TATGGTCCGA 
CTGGACCAAT TCCAGGACTT TTGTGATGAT GACGAAGAAG ATGATTGGTG TTCAACATGC 
TCTTCTTCAT CTTCAGAATC TGAGGAGGAG GGATTCTTTC TAGGTCAGCC AATCCCTAAG 
CCCCGATCCC AAAGTTTTCA TTATTACACA GAGGACTATC CGACTCGGGT CACGGCTTTA 
ACGCCTCCTT TTGGCTCAAG GACCAAACTG AAAAAGAAAG GTCACAAGGG CAAGAACTGC 
ATAATTTCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prickle1b ( XM_005164634.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164691.1 Danio rerio prickle homolog 1b (prickle1b), transcript variant X1, mRNA.

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CloneID	ODa09250
Clone ID Related Accession (Same CDS sequence)	XM_005164634.4 , XM_005164634.3
Accession Version	XM_005164634.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2592bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	prickle-like protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005164634.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCTTG AGACTACATC TGGGATCTGG CCCATTACTC GGGCACCGGA CATGGACTCG 
AGAGGAGCCA AGGCATTTGG GTTTCAGAGG AGTTCAACGT CTGATGACGA CTCTGGATGT 
GCTCTTGAGG AGTACACATG GGTTCCTCCT GGACTACGGG CTGAGCAGGT TCAGATCTAC 
TTTTCGTGCC TACCAGAAGA GAAGGTGCCA TATGTCAACA GCCCTGGAGA GAAGCACAGG 
ATCAGACAAC TACTCTATCA GCTGCCACCC CACGACAATG AGATTCGATA CTGCCAGTCC 
CTCAGTGATG AAGAGAGACG AGAACTCCAC ATGTTCAGCA TGCAAAGAAA GAAAGAGGCA 
CTTGGGAGGG GGACCCCAAA ACTGCTTCCC AGAGCTCTGC AACATAATTG TGAGCATTGT 
AAAGAGAACA TCAACGGTGG AGAGATGGCA GTGTTTGCTT CGAGAGCAGG CCCCGGCCCT 
TGCTGGCACC CAGCATGCTT TACTTGCTAC ACCTGTCATG AACTTCTGGT GGACCTCATC 
TATTTCTACC ACAATGGCAA TATCCACTGC GGCAGACATC ATGCAGAGCT TCTGAAGCCA 
CGTTGCTCAG CATGTGATGA GATTATCTTT GCTGACGAGT GTACCGAAGC TGAGGGTCGT 
CACTGGCACA TGAAACATTT TTCATGCTTT GAGTGCGAGA CTATTTTAGG AGGACAGAGG 
TACATCATGA AAGATGGCCG ACCCTTCTGT TGTGGATGCT TTGAGTCATT GTATGCAGAG 
TACTGCGAGG CCTGTGGAGA GAATATTGGA GTCGACCATG CTCAAATGAC GTACGAGGGT 
GTACATTGGC ACGCTACTGA CAAATGCTTT TGTTGTGCAC AATGTAAAAC CTCTCTGCTG 
GGCTGTCCCT TCCTTCCAAA AGATGGTCGT ATCTACTGCT CTAAGGACTG TAGCCTAGGT 
GAGGATGTCC AGGCTTCTGA TTCTTCAGAC TCCGCTTTTC AGTCCGCCAG ATCACGGGAA 
TCTCGACGCA GTATTCGAAT GGGCAAGAGT AGTCGCCCCA CAGAACAGTG CCAGCAGACA 
AAATTGTACT CAGCAACAGA TTACAGGACC TCTAGTCTAT GTGGCGACAA CACAGAGACA 
ATGTGTCATC AGCTGGAGAG AATCAACTTT ACAGAAGACA ACTACTGGAG AGAGCGAGAT 
GAGCAGGAAG CCCCTGAGGA CTCTGTGGAA GATTGGGCTG AGCATGAGGA CTACATGACT 
CAGCTGCTCC TGAAATTTGG AGACCGTGGT GTCATCCACG CAGAGGACAG CAGGGCAGCA 
GAGCCATGGA TGGTGAAAGA CACCGAAACC AACCGAACCA AACCAGAGAT AAAGATGAGC 
GGCCACAATT TGGCAAGTAA AAAGCACCAC ACTGACATGT ATTGGGCCCA GTCTCAGGAT 
GGACTTGGAG ACTCTGCTTA TGGTAGTCAC CCTGGACCAG CTAGCGCTAG AAAGATTCAA 
GAGCTGGAGA TGGATCAGGG AACAGTACCT GGATTTTGGT CTGAGCCAAG GCAATGGTAT 
GAAGACTCAT TAGAGTGCAT TACAAGTGAA CTCCGGAAGG CAGACCACAA TATTGGAGAT 
TCTATGGATT CTCTGGCTCT CTCAAATATT ACAGGAGCTT CAGTGGATGG GGACAGCAAG 
GACAGACAGA CCATTTACTC CCTCCAAAAC ATGAAAGACC AAGAAAGAGA GATAGAAGAG 
TGTGATAACA TTAGTAACGT AGGCACACTA AACTCCTCAA TGCTCCACAG AAGTGCTAAT 
TCCCTGAGGA GTTTAAACTC CGAGCTAGGT CATGAGGAGA CACTGGGGCA GGGAGCAAGA 
GAAGACGATG AAGAGGACAG AATACCACCA TATTTTGGAG CAGAACCACC ATCTCCTCTG 
AATCTTCCAG TGCTGAGAAG GACCAAGTCA CAATCTCAGC CGCAATCCAG ATCTCAGCAA 
GTAAAGTTCT CTGATGATGT TCTGGAGAAA GGGTATTATG GTGCACTGGG CTTAAGGCAA 
CCACCAATGA GTGAGAGGAC ACGCAGGAGG ACCTTTCACA TAGAGGAGGA GGCAGCGGCA 
GCAGCAGAGA GACGTCCTGC TAACAGACAT CACCACAAAC GCAGGCACAG TCGGAAAACG 
CGTTCAGAAA ATGCATTGCA CCTGCCTTCC AAAAATCGTG GGAAACTGCA TGTTGTGCCT 
CAGGTAATCA GCTCAAACAT GCCAGAGCCC AGGAATGGTA TTCTACTAAA TCACTCGGCG 
CACAGTCAGC ACCCTTATCG TCAAACCACC TCAGACTTTC CTCAGCAAGG TATGGTCCGA 
CTGGACCAAT TCCAGGACTT TTGTGATGAT GACGAAGAAG ATGATTGGTG TTCAACATGC 
TCTTCTTCAT CTTCAGAATC TGAGGAGGAG GGATTCTTTC TAGGTCAGCC AATCCCTAAG 
CCCCGATCCC AAAGTTTTCA TTATTACACA GAGGACTATC CGACTCGGGT CACGGCTTTA 
ACGCCTCCTT TTGGCTCAAG GACCAAACTG AAAAAGAAAG GTCACAAGGG CAAGAACTGC 
ATAATTTCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164691.1
CDS	246..2837
Translation	MSLETTSGIWPITRAPDMDSRGAKAFGFQRSSTSDDDSGCALEEYTWVPPGLRAEQVQIYFSCLPEEKVPYVNSPGEKHRIRQLLYQLPPHDNEIRYCQSLSDEERRELHMFSMQRKKEALGRGTPKLLPRALQHNCEHCKENINGGEMAVFASRAGPGPCWHPACFTCYTCHELLVDLIYFYHNGNIHCGRHHAELLKPRCSACDEIIFADECTEAEGRHWHMKHFSCFECETILGGQRYIMKDGRPFCCGCFESLYAEYCEACGENIGVDHAQMTYEGVHWHATDKCFCCAQCKTSLLGCPFLPKDGRIYCSKDCSLGEDVQASDSSDSAFQSARSRESRRSIRMGKSSRPTEQCQQTKLYSATDYRTSSLCGDNTETMCHQLERINFTEDNYWRERDEQEAPEDSVEDWAEHEDYMTQLLLKFGDRGVIHAEDSRAAEPWMVKDTETNRTKPEIKMSGHNLASKKHHTDMYWAQSQDGLGDSAYGSHPGPASARKIQELEMDQGTVPGFWSEPRQWYEDSLECITSELRKADHNIGDSMDSLALSNITGASVDGDSKDRQTIYSLQNMKDQEREIEECDNISNVGTLNSSMLHRSANSLRSLNSELGHEETLGQGAREDDEEDRIPPYFGAEPPSPLNLPVLRRTKSQSQPQSRSQQVKFSDDVLEKGYYGALGLRQPPMSERTRRRTFHIEEEAAAAAERRPANRHHHKRRHSRKTRSENALHLPSKNRGKLHVVPQVISSNMPEPRNGILLNHSAHSQHPYRQTTSDFPQQGMVRLDQFQDFCDDDEEDDWCSTCSSSSSESEEEGFFLGQPIPKPRSQSFHYYTEDYPTRVTALTPPFGSRTKLKKKGHKGKNCIIS

Target ORF information:

RefSeq Version	XM_005164634.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio prickle homolog 1b (prickle1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164634.3

ORF Insert Sequence:

ATGAGTCTTG AGACTACATC TGGGATCTGG CCCATTACTC GGGCACCGGA CATGGACTCG 
AGAGGAGCCA AGGCATTTGG GTTTCAGAGG AGTTCAACGT CTGATGACGA CTCTGGATGT 
GCTCTTGAGG AGTACACATG GGTTCCTCCT GGACTACGGG CTGAGCAGGT TCAGATCTAC 
TTTTCGTGCC TACCAGAAGA GAAGGTGCCA TATGTCAACA GCCCTGGAGA GAAGCACAGG 
ATCAGACAAC TACTCTATCA GCTGCCACCC CACGACAATG AGATTCGATA CTGCCAGTCC 
CTCAGTGATG AAGAGAGACG AGAACTCCAC ATGTTCAGCA TGCAAAGAAA GAAAGAGGCA 
CTTGGGAGGG GGACCCCAAA ACTGCTTCCC AGAGCTCTGC AACATAATTG TGAGCATTGT 
AAAGAGAACA TCAACGGTGG AGAGATGGCA GTGTTTGCTT CGAGAGCAGG CCCCGGCCCT 
TGCTGGCACC CAGCATGCTT TACTTGCTAC ACCTGTCATG AACTTCTGGT GGACCTCATC 
TATTTCTACC ACAATGGCAA TATCCACTGC GGCAGACATC ATGCAGAGCT TCTGAAGCCA 
CGTTGCTCAG CATGTGATGA GATTATCTTT GCTGACGAGT GTACCGAAGC TGAGGGTCGT 
CACTGGCACA TGAAACATTT TTCATGCTTT GAGTGCGAGA CTATTTTAGG AGGACAGAGG 
TACATCATGA AAGATGGCCG ACCCTTCTGT TGTGGATGCT TTGAGTCATT GTATGCAGAG 
TACTGCGAGG CCTGTGGAGA GAATATTGGA GTCGACCATG CTCAAATGAC GTACGAGGGT 
GTACATTGGC ACGCTACTGA CAAATGCTTT TGTTGTGCAC AATGTAAAAC CTCTCTGCTG 
GGCTGTCCCT TCCTTCCAAA AGATGGTCGT ATCTACTGCT CTAAGGACTG TAGCCTAGGT 
GAGGATGTCC AGGCTTCTGA TTCTTCAGAC TCCGCTTTTC AGTCCGCCAG ATCACGGGAA 
TCTCGACGCA GTATTCGAAT GGGCAAGAGT AGTCGCCCCA CAGAACAGTG CCAGCAGACA 
AAATTGTACT CAGCAACAGA TTACAGGACC TCTAGTCTAT GTGGCGACAA CACAGAGACA 
ATGTGTCATC AGCTGGAGAG AATCAACTTT ACAGAAGACA ACTACTGGAG AGAGCGAGAT 
GAGCAGGAAG CCCCTGAGGA CTCTGTGGAA GATTGGGCTG AGCATGAGGA CTACATGACT 
CAGCTGCTCC TGAAATTTGG AGACCGTGGT GTCATCCACG CAGAGGACAG CAGGGCAGCA 
GAGCCATGGA TGGTGAAAGA CACCGAAACC AACCGAACCA AACCAGAGAT AAAGATGAGC 
GGCCACAATT TGGCAAGTAA AAAGCACCAC ACTGACATGT ATTGGGCCCA GTCTCAGGAT 
GGACTTGGAG ACTCTGCTTA TGGTAGTCAC CCTGGACCAG CTAGCGCTAG AAAGATTCAA 
GAGCTGGAGA TGGATCAGGG AACAGTACCT GGATTTTGGT CTGAGCCAAG GCAATGGTAT 
GAAGACTCAT TAGAGTGCAT TACAAGTGAA CTCCGGAAGG CAGACCACAA TATTGGAGAT 
TCTATGGATT CTCTGGCTCT CTCAAATATT ACAGGAGCTT CAGTGGATGG GGACAGCAAG 
GACAGACAGA CCATTTACTC CCTCCAAAAC ATGAAAGACC AAGAAAGAGA GATAGAAGAG 
TGTGATAACA TTAGTAACGT AGGCACACTA AACTCCTCAA TGCTCCACAG AAGTGCTAAT 
TCCCTGAGGA GTTTAAACTC CGAGCTAGGT CATGAGGAGA CACTGGGGCA GGGAGCAAGA 
GAAGACGATG AAGAGGACAG AATACCACCA TATTTTGGAG CAGAACCACC ATCTCCTCTG 
AATCTTCCAG TGCTGAGAAG GACCAAGTCA CAATCTCAGC CGCAATCCAG ATCTCAGCAA 
GTAAAGTTCT CTGATGATGT TCTGGAGAAA GGGTATTATG GTGCACTGGG CTTAAGGCAA 
CCACCAATGA GTGAGAGGAC ACGCAGGAGG ACCTTTCACA TAGAGGAGGA GGCAGCGGCA 
GCAGCAGAGA GACGTCCTGC TAACAGACAT CACCACAAAC GCAGGCACAG TCGGAAAACG 
CGTTCAGAAA ATGCATTGCA CCTGCCTTCC AAAAATCGTG GGAAACTGCA TGTTGTGCCT 
CAGGTAATCA GCTCAAACAT GCCAGAGCCC AGGAATGGTA TTCTACTAAA TCACTCGGCG 
CACAGTCAGC ACCCTTATCG TCAAACCACC TCAGACTTTC CTCAGCAAGG TATGGTCCGA 
CTGGACCAAT TCCAGGACTT TTGTGATGAT GACGAAGAAG ATGATTGGTG TTCAACATGC 
TCTTCTTCAT CTTCAGAATC TGAGGAGGAG GGATTCTTTC TAGGTCAGCC AATCCCTAAG 
CCCCGATCCC AAAGTTTTCA TTATTACACA GAGGACTATC CGACTCGGGT CACGGCTTTA 
ACGCCTCCTT TTGGCTCAAG GACCAAACTG AAAAAGAAAG GTCACAAGGG CAAGAACTGC 
ATAATTTCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Prickle1 is required for EMT and migration of zebrafish cranial neural crest.
Developmental biology448(1)16-35(2019 04)
Ahsan K,Singh N,Rocha M,Huang C,Prince VE

Planar cell polarity genes Frizzled3a, Vangl2, and Scribble are required for spinal commissural axon guidance.
BMC neuroscience17(1)83(2016 12)
Sun SD,Purdy AM,Walsh GS

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

CDX4 and retinoic acid interact to position the hindbrain-spinal cord transition.
Developmental biology410(2)178-189(2016 Feb)
Chang J,Skromne I,Ho RK

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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prickle1b cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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