SRGAP1B cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	SRGAP1B
Entrez Gene ID	555788
Full Name	SLIT-ROBO Rho GTPase activating protein 1b
Synonyms	si:ch211-125a15.2,srgap2b
General protein information	Preferred Names SLIT-ROBO Rho GTPase activating protein 1b Names SLIT-ROBO Rho GTPase-activating protein 1 SLIT-ROBO Rho GTPase activating protein 2b
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005164583.4	XP_005164640.1	SLIT-ROBO Rho GTPase-activating protein 1 isoform X1
NM_001030095.1	NP_001025266.1	SLIT-ROBO Rho GTPase-activating protein 1
XM_005164583.3	XP_005164640.1	SLIT-ROBO Rho GTPase-activating protein 1 isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Dissection of Glomerular Transcriptional Profile in Patients With Diabetic Nephropathy: SRGAP2a Protects Podocyte Structure and Function.
Pan Y, Jiang S, Hou Q, Qiu D, Shi J, Wang L, Chen Z, Zhang M, Duan A, Qin W, Zen K, Liu Z
Diabetes67(4)717-730(2018 04)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Eumetazoan cryptochrome phylogeny and evolution.
Haug MF, Gesemann M, Lazović V, Neuhauss SC
Genome biology and evolution7(2)601-19(2015 Jan)

On the crucial cerebellar wound healing-related pathways and their cross-talks after traumatic brain injury in Danio rerio.
Wu CC, Tsai TH, Chang C, Lee TT, Lin C, Cheng IH, Sun MC, Chuang YJ, Chen BS
PloS one9(6)e97902(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SRGAP1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SRGAP1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa32117	NM_001030095.1 Latest version!	Danio rerio SLIT-ROBO Rho GTPase activating protein 1b (srgap1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa09197	XM_005164583.4 Latest version!	Danio rerio SLIT-ROBO Rho GTPase activating protein 1b (srgap1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
ODa09197	XM_005164583.3	Danio rerio SLIT-ROBO Rho GTPase activating protein 1b (srgap1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SRGAP1B ( NM_001030095.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001025266.1 Danio rerio SLIT-ROBO Rho GTPase activating protein 1b (srgap1b), mRNA.

Price & Availability↑

CloneID	ODa32117
Clone ID Related Accession (Same CDS sequence)	NM_001030095.1
Accession Version	NM_001030095.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576252800000
Organism	Danio rerio(zebrafish)
Product	SLIT-ROBO Rho GTPase-activating protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX255922.4. On Aug 6, 2005 this sequence version replaced XM_678406.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA3505376, SAMEA3505378 [ECO:0000350] ##Evidence-Data-END##

ATGTCTAACT CGACGAAGAG CAAAAAGGAA AAGGAAATCA TTACGGAGTA TGAGAGCCAA 
GTGAAAGAGA TTCGGTCTCA GCTGGTGGAG CAGCAGAAAT GTCTGGAGCA GCAGACGGAC 
ATGCGGGTTC AGCTCCTCCA GGACTTGCAG GATTTCTTCA GGAAGAAAGC CGAGATCGAG 
AGCGAATATT CACGCAACCT GGAGAAGCTC GCCGAACGCT TTATGGCCAA AACCCGCAGC 
ACCAAGGACC ACCAACAGTA CAAGAAGGAC CAGAATCTGC TGTCTCCAGT GAACTGCTGG 
TATCTGCTGC TGAACCAGGT GAGGAGGGAG AGTAAAGACC ACGCCACACT GAGTGACCTT 
TACCTCAACA ACGTCATCAC TCGCTTCACA CATGTCAGCG AGGACAACAT GCGTCTGCTC 
AAGAAGAGCA AAGAGATCAT TTTCCAGCTG CAAGAAGACC TGATGAAGAT CCTTAACGAA 
CTCTACACGG TGATGAAGAC GTATCACATG TATCACACCG AGACTCTGAG TGCCGAGAAT 
AAGCTAAAAG AGGCGGAGAG GCAGGGTGAG CGACAGGGCC GAGGCGGAGA GGCCGTCTTC 
AGTCTGCGCA CCGAAGACCG CCACCAACGG CGCAACGCGG CCCGCAAGAT CCAGAAGATG 
AAGGAGAAGC GCCAAGCCAA GTACTCGGAG AACAAACTGA AGGTTATGAA AGCACGGAAC 
GAGTATCTGT TGACCCTGGA GGCCACGAAC GCTTCTCTAT TTAAATATTA CATCCACGAC 
CTGTCGCATC TGACTGACTG TTGTGATCTC GGCTATCATG CGGGCTTGAG TCGGGCATTG 
CGGACCTACC TATCAGCCGA GTACAGTTTG GAAACATCTC GACATGAGGG CCTGGATATT 
TTGGAGGGGG CCGTGGAAGG GCTGGACCCC GGTAATGACC GGCAGCGATT TATGGAGACT 
CACCCAACAT CCTTCACTCC TCCTACACGC TTTACCTTTC AGCCACACAT GGGAGACCAG 
GTGAGTCAAA TCACAGCACT GCCGCAGGTG CAGGCCGAAC TGCTCCTCCG ATTCCAGCAG 
CTGCAGTCGC GCCTCTCCAC ACTCAAGATC GAAAATGAGG AGGTGAAAAA GACATCAGAG 
GCCACACTCA GCACCATCCA GGACATGGTG AACATGGAGG ACTACGACGT TTCTGAGAGT 
TTCCAGCACA GTCGCTCCAC TGAGTCGGTC AAATCCAGCG TATCAGACAC TTACCTCAGC 
AAACCCAGCC TGGCCAAGAG ACGCGCCAAC CAGCAGGAAA CAGAGCTCTT CTACTTCACC 
AAACTGAAGG AGTATCTGGA AGGCAGTAAT CTCATAGCTA AACTGCAAGC CAAGCATGAC 
ATGCTGAAGA GGAGCATAGC TGAAGGATAC AGGGCAGACA TCATGACAAC TAGGTACAGT 
ATCTCCAATC ATCCCTGTCT GACCTTCTTT AATGCTCTCT TCACATTCAC TGATTCTGGG 
AAAGCCATTC CTCTGATTGT AGAAAGCTGC ATTCGCTTTA TTAACCTATA TGGTCTGCAA 
CACCAGGGTA TTTTCCGGGT TTCAGGGTCA CAAGTGGAGG TCAATGACAT CAAAAACTCT 
TTTGAACGAG GTAATGATCC GCTGACTGAT GAGGAGAATA ATCATGACAT TAACTCAGTG 
GCAGGCGTCC TAAAAATGTA CTTCCGCAAC CTGGACAATC CAATTTTCCC AAAGGAGAAG 
TTTAACGATC TGATCGCCTG CGTTCGAACT GAGAGTTTGT ATGAGCGTGC CCTCTCCATC 
CGCAAGATCC TCACCACAAT GCCACGGCCA ACACTGGTGC TTATGCGATA CCTGTTTGCA 
TTCCTCAACC ATTTGTCCCA GTACAGTGAC GAGAACATGA TGGACCCTGG AAACCTGGCT 
ATTTGCTTTG GACCCACTTT AATGCCCACT CCGGACCTCC TAGACCAGGT CTCCTGTCAG 
GCCCATGTCA ACGAGATTGT CAAAACCATC ATCATCCACC ATGAAACCAT CTTCCCTGAC 
ACCAAAGAGC TGGAGGGACC CGTCTATGAG AAGTGCATGA GCAACACTGA GTACTGTGAA 
AGTCCGTACA GTGAACCTGG AGCGTTTGAG GAGGGGGAGC AGGATGGAGG GACGGAAACA 
CAGACGAGTG AGGAGGAGGG TGAGCCTGTG GAGGCGATAG CCAAGTTTGA CTATGTGGGC 
CGTTCGGGAC GGGAGCTCAC CTTTAAGAAA GGCTCCACTC TGCTGCTCTT CCAGAGGGCT 
TCTGAAGACT GGTGGGAGGG ACGACACAAC GGCACCGATG GACTCATACC TCACCAATAC 
GTCACGCTGC AGGAACACCG GAAGCGGACT GAAGCTCTCC TGCGCCGTGT CCCAGGACGT 
CTGAGCGATA ATCACTGCCA TGGGAATGGA CTGGAACGCA GTTCTCCTCC GGTAACGGTG 
ACGGTAACAG GTCATTTCAG CCCGCGGGAG CTGCTGCGGG GGAACAGCTG TGCGCTGGAC 
AGTCCTGAGC ACCACAGACG CCCGGGACTC AATGGTTCCT CCAATGGGAA CATCACCTCC 
AGTATCAGCC GACACGAGTC CCTGCGCAGA GAGCGAGAGA GTCCACCGAT ACGCCAGTCT 
CCATCTGCTC TGCACTGCGG GTTACCCATT CAACGCTCTC AGACGCTCAA CCCGCATACA 
ATGGCACAGG ACATAGAGGA GACCATGAGT CTGGCCCTGA ACGAGCTGCG TGAGCTGGAA 
AGGCAGAACG GCAAAACCGC AGCTCCTGAC CTGGTGCTGG ACACCCTGGA GCAGGTGAAA 
AGCAGCCCGT CCTGCCCATC AGGCTCCACA CCCTCCAGCC TGTCCAGTCC GTCCGACTCC 
CCGAGCCCCT TGAGCCCCAT CAGCCCCCTC CCTCCTCTCC CTCCACTTCC CGGCCCTTCG 
TCCCGCCGCA CAAATGACCT GGTGGCCACC TTCAAACCCA TCGCCTCACC CCGTATGGGT 
GTAACCCTCC GCCCACCAGC TTTACGCCCT AAACCGCCCA TTCTCCCCAA GGCCAACCCT 
CCCCCTTCCC AGCAGCCCCA AAATCCAGAC AAATCCGGCA CTATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001025266.1
CDS	466..3573
Translation	MSNSTKSKKEKEIITEYESQVKEIRSQLVEQQKCLEQQTDMRVQLLQDLQDFFRKKAEIESEYSRNLEKLAERFMAKTRSTKDHQQYKKDQNLLSPVNCWYLLLNQVRRESKDHATLSDLYLNNVITRFTHVSEDNMRLLKKSKEIIFQLQEDLMKILNELYTVMKTYHMYHTETLSAENKLKEAERQGERQGRGGEAVFSLRTEDRHQRRNAARKIQKMKEKRQAKYSENKLKVMKARNEYLLTLEATNASLFKYYIHDLSHLTDCCDLGYHAGLSRALRTYLSAEYSLETSRHEGLDILEGAVEGLDPGNDRQRFMETHPTSFTPPTRFTFQPHMGDQVSQITALPQVQAELLLRFQQLQSRLSTLKIENEEVKKTSEATLSTIQDMVNMEDYDVSESFQHSRSTESVKSSVSDTYLSKPSLAKRRANQQETELFYFTKLKEYLEGSNLIAKLQAKHDMLKRSIAEGYRADIMTTRYSISNHPCLTFFNALFTFTDSGKAIPLIVESCIRFINLYGLQHQGIFRVSGSQVEVNDIKNSFERGNDPLTDEENNHDINSVAGVLKMYFRNLDNPIFPKEKFNDLIACVRTESLYERALSIRKILTTMPRPTLVLMRYLFAFLNHLSQYSDENMMDPGNLAICFGPTLMPTPDLLDQVSCQAHVNEIVKTIIIHHETIFPDTKELEGPVYEKCMSNTEYCESPYSEPGAFEEGEQDGGTETQTSEEEGEPVEAIAKFDYVGRSGRELTFKKGSTLLLFQRASEDWWEGRHNGTDGLIPHQYVTLQEHRKRTEALLRRVPGRLSDNHCHGNGLERSSPPVTVTVTGHFSPRELLRGNSCALDSPEHHRRPGLNGSSNGNITSSISRHESLRRERESPPIRQSPSALHCGLPIQRSQTLNPHTMAQDIEETMSLALNELRELERQNGKTAAPDLVLDTLEQVKSSPSCPSGSTPSSLSSPSDSPSPLSPISPLPPLPPLPGPSSRRTNDLVATFKPIASPRMGVTLRPPALRPKPPILPKANPPPSQQPQNPDKSGTM

Target ORF information:

RefSeq Version	NM_001030095.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SLIT-ROBO Rho GTPase activating protein 1b (srgap1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001030095.1

ORF Insert Sequence:

ATGTCTAACT CGACGAAGAG CAAAAAGGAA AAGGAAATCA TTACGGAGTA TGAGAGCCAA 
GTGAAAGAGA TTCGGTCTCA GCTGGTGGAG CAGCAGAAAT GTCTGGAGCA GCAGACGGAC 
ATGCGGGTTC AGCTCCTCCA GGACTTGCAG GATTTCTTCA GGAAGAAAGC CGAGATCGAG 
AGCGAATATT CACGCAACCT GGAGAAGCTC GCCGAACGCT TTATGGCCAA AACCCGCAGC 
ACCAAGGACC ACCAACAGTA CAAGAAGGAC CAGAATCTGC TGTCTCCAGT GAACTGCTGG 
TATCTGCTGC TGAACCAGGT GAGGAGGGAG AGTAAAGACC ACGCCACACT GAGTGACCTT 
TACCTCAACA ACGTCATCAC TCGCTTCACA CATGTCAGCG AGGACAACAT GCGTCTGCTC 
AAGAAGAGCA AAGAGATCAT TTTCCAGCTG CAAGAAGACC TGATGAAGAT CCTTAACGAA 
CTCTACACGG TGATGAAGAC GTATCACATG TATCACACCG AGACTCTGAG TGCCGAGAAT 
AAGCTAAAAG AGGCGGAGAG GCAGGGTGAG CGACAGGGCC GAGGCGGAGA GGCCGTCTTC 
AGTCTGCGCA CCGAAGACCG CCACCAACGG CGCAACGCGG CCCGCAAGAT CCAGAAGATG 
AAGGAGAAGC GCCAAGCCAA GTACTCGGAG AACAAACTGA AGGTTATGAA AGCACGGAAC 
GAGTATCTGT TGACCCTGGA GGCCACGAAC GCTTCTCTAT TTAAATATTA CATCCACGAC 
CTGTCGCATC TGACTGACTG TTGTGATCTC GGCTATCATG CGGGCTTGAG TCGGGCATTG 
CGGACCTACC TATCAGCCGA GTACAGTTTG GAAACATCTC GACATGAGGG CCTGGATATT 
TTGGAGGGGG CCGTGGAAGG GCTGGACCCC GGTAATGACC GGCAGCGATT TATGGAGACT 
CACCCAACAT CCTTCACTCC TCCTACACGC TTTACCTTTC AGCCACACAT GGGAGACCAG 
GTGAGTCAAA TCACAGCACT GCCGCAGGTG CAGGCCGAAC TGCTCCTCCG ATTCCAGCAG 
CTGCAGTCGC GCCTCTCCAC ACTCAAGATC GAAAATGAGG AGGTGAAAAA GACATCAGAG 
GCCACACTCA GCACCATCCA GGACATGGTG AACATGGAGG ACTACGACGT TTCTGAGAGT 
TTCCAGCACA GTCGCTCCAC TGAGTCGGTC AAATCCAGCG TATCAGACAC TTACCTCAGC 
AAACCCAGCC TGGCCAAGAG ACGCGCCAAC CAGCAGGAAA CAGAGCTCTT CTACTTCACC 
AAACTGAAGG AGTATCTGGA AGGCAGTAAT CTCATAGCTA AACTGCAAGC CAAGCATGAC 
ATGCTGAAGA GGAGCATAGC TGAAGGATAC AGGGCAGACA TCATGACAAC TAGGTACAGT 
ATCTCCAATC ATCCCTGTCT GACCTTCTTT AATGCTCTCT TCACATTCAC TGATTCTGGG 
AAAGCCATTC CTCTGATTGT AGAAAGCTGC ATTCGCTTTA TTAACCTATA TGGTCTGCAA 
CACCAGGGTA TTTTCCGGGT TTCAGGGTCA CAAGTGGAGG TCAATGACAT CAAAAACTCT 
TTTGAACGAG GTAATGATCC GCTGACTGAT GAGGAGAATA ATCATGACAT TAACTCAGTG 
GCAGGCGTCC TAAAAATGTA CTTCCGCAAC CTGGACAATC CAATTTTCCC AAAGGAGAAG 
TTTAACGATC TGATCGCCTG CGTTCGAACT GAGAGTTTGT ATGAGCGTGC CCTCTCCATC 
CGCAAGATCC TCACCACAAT GCCACGGCCA ACACTGGTGC TTATGCGATA CCTGTTTGCA 
TTCCTCAACC ATTTGTCCCA GTACAGTGAC GAGAACATGA TGGACCCTGG AAACCTGGCT 
ATTTGCTTTG GACCCACTTT AATGCCCACT CCGGACCTCC TAGACCAGGT CTCCTGTCAG 
GCCCATGTCA ACGAGATTGT CAAAACCATC ATCATCCACC ATGAAACCAT CTTCCCTGAC 
ACCAAAGAGC TGGAGGGACC CGTCTATGAG AAGTGCATGA GCAACACTGA GTACTGTGAA 
AGTCCGTACA GTGAACCTGG AGCGTTTGAG GAGGGGGAGC AGGATGGAGG GACGGAAACA 
CAGACGAGTG AGGAGGAGGG TGAGCCTGTG GAGGCGATAG CCAAGTTTGA CTATGTGGGC 
CGTTCGGGAC GGGAGCTCAC CTTTAAGAAA GGCTCCACTC TGCTGCTCTT CCAGAGGGCT 
TCTGAAGACT GGTGGGAGGG ACGACACAAC GGCACCGATG GACTCATACC TCACCAATAC 
GTCACGCTGC AGGAACACCG GAAGCGGACT GAAGCTCTCC TGCGCCGTGT CCCAGGACGT 
CTGAGCGATA ATCACTGCCA TGGGAATGGA CTGGAACGCA GTTCTCCTCC GGTAACGGTG 
ACGGTAACAG GTCATTTCAG CCCGCGGGAG CTGCTGCGGG GGAACAGCTG TGCGCTGGAC 
AGTCCTGAGC ACCACAGACG CCCGGGACTC AATGGTTCCT CCAATGGGAA CATCACCTCC 
AGTATCAGCC GACACGAGTC CCTGCGCAGA GAGCGAGAGA GTCCACCGAT ACGCCAGTCT 
CCATCTGCTC TGCACTGCGG GTTACCCATT CAACGCTCTC AGACGCTCAA CCCGCATACA 
ATGGCACAGG ACATAGAGGA GACCATGAGT CTGGCCCTGA ACGAGCTGCG TGAGCTGGAA 
AGGCAGAACG GCAAAACCGC AGCTCCTGAC CTGGTGCTGG ACACCCTGGA GCAGGTGAAA 
AGCAGCCCGT CCTGCCCATC AGGCTCCACA CCCTCCAGCC TGTCCAGTCC GTCCGACTCC 
CCGAGCCCCT TGAGCCCCAT CAGCCCCCTC CCTCCTCTCC CTCCACTTCC CGGCCCTTCG 
TCCCGCCGCA CAAATGACCT GGTGGCCACC TTCAAACCCA TCGCCTCACC CCGTATGGGT 
GTAACCCTCC GCCCACCAGC TTTACGCCCT AAACCGCCCA TTCTCCCCAA GGCCAACCCT 
CCCCCTTCCC AGCAGCCCCA AAATCCAGAC AAATCCGGCA CTATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SRGAP1B ( XM_005164583.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005164640.1 Danio rerio SLIT-ROBO Rho GTPase activating protein 1b (srgap1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa09197
Clone ID Related Accession (Same CDS sequence)	XM_005164583.4 , XM_005164583.3
Accession Version	XM_005164583.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	SLIT-ROBO Rho GTPase-activating protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005164583.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAACT CGACGAAGAG CAAAAAGGAA AAGGAAATCA TTACGGAGTA TGAGAGCCAA 
GTGAAAGAGA TTCGGTCTCA GCTGGTGGAG CAGCAGAAAT GTCTGGAGCA GCAGACGGAC 
ATGCGGGTTC AGCTCCTCCA GGACTTGCAG GATTTCTTCA GGAAGAAAGC CGAGATCGAG 
AGCGAATATT CACGCAACCT GGAGAAGCTC GCCGAACGCT TTATGGCCAA AACCCGCAGC 
ACCAAGGACC ACCAACAGTA CAAGAAGGAC CAGAATCTGC TGTCTCCAGT GAACTGCTGG 
TATCTGCTGC TGAACCAGGT GAGGAGGGAG AGTAAAGACC ACGCCACACT GAGTGACCTT 
TACCTCAACA ACGTCATCAC TCGCTTCACA CATGTCAGCG AGGACAACAT GCGTCTGCTC 
AAGAAGAGCA AAGAGATCAT TTTCCAGCTG CAAGAAGACC TGATGAAGAT CCTTAACGAA 
CTCTACACGG TGATGAAGAC GTATCACATG TATCACACCG AGACTCTGAG TGCCGAGAAT 
AAGCTAAAAG AGGCGGAGAG GCAGGGTGAG CGACAGGGCC GAGGCGGAGA GGCCGTCTTC 
AGTCTGCGCA CCGAAGACCG CCACCAACGG CGCAACGCGG CCCGCAAGAT CCAGAAGATG 
AAGGAGAAGC GCCAAGCCAA GTACTCGGAG AACAAACTGA AGGTTATGAA AGCACGGAAC 
GAGTATCTGT TGACCCTGGA GGCCACGAAC GCTTCTCTAT TTAAATATTA CATCCACGAC 
CTGTCGCATC TGACTGACTG TTGTGATCTC GGCTATCATG CGGGCTTGAG TCGGGCATTG 
CGGACCTACC TATCAGCCGA GTACAGTTTG GAAACATCTC GACATGAGGG CCTGGATATT 
TTGGAGGGGG CCGTGGAAGG GCTGGACCCC GGTAATGACC GGCAGCGATT TATGGAGACT 
CACCCAACAT CCTTCACTCC TCCTACACGC TTTACCTTTC AGCCACACAT GGGAGACCAG 
GTGAGTCAAA TCACAGCACT GCCGCAGGTG CAGGCCGAAC TGCTCCTCCG ATTCCAGCAG 
CTGCAGTCGC GCCTCTCCAC ACTCAAGATC GAAAATGAGG AGGTGAAAAA GACATCAGAG 
GCCACACTCA GCACCATCCA GGACATGGTG AACATGGAGG ACTACGACGT TTCTGAGAGT 
TTCCAGCACA GTCGCTCCAC TGAGTCGGTC AAATCCAGCG TATCAGACAC TTACCTCAGC 
AAACCCAGCC TGGCCAAGAG ACGCGCCAAC CAGCAGGAAA CAGAGCTCTT CTACTTCACC 
AAACTGAAGG AGTATCTGGA AGGCAGTAAT CTCATAGCTA AACTGCAAGC CAAGCATGAC 
ATGCTGAAGA GGAGCATAGC TGAAGGATAC AGGGCAGACA TCATGACAAC TAGCCGTCGA 
CGAAACTCTC ACAGCAGAAA CCAGGATTCT GGGAAAGCCA TTCCTCTGAT TGTAGAAAGC 
TGCATTCGCT TTATTAACCT ATATGGTCTG CAACACCAGG GTATTTTCCG GGTTTCAGGG 
TCACAAGTGG AGGTCAATGA CATCAAAAAC TCTTTTGAAC GAGGTAATGA TCCGCTGACT 
GATGAGGAGA ATAATCATGA CATTAACTCA GTGGCAGGCG TCCTAAAAAT GTACTTCCGC 
AACCTGGACA ATCCAATTTT CCCAAAGGAG AAGTTTAACG ATCTGATCGC CTGCGTTCGA 
ACTGAGAGTT TGTATGAGCG TGCCCTCTCC ATCCGCAAGA TCCTCACCAC AATGCCACGG 
CCAACACTGG TGCTTATGCG ATACCTGTTT GCATTCCTCA ACCATTTGTC CCAGTACAGT 
GACGAGAACA TGATGGACCC TGGAAACCTG GCTATTTGCT TTGGACCCAC TTTAATGCCC 
ACTCCGGACC TCCTAGACCA GGTCTCCTGT CAGGCCCATG TCAACGAGAT TGTCAAAACC 
ATCATCATCC ACCATGAAAC CATCTTCCCT GACACCAAAG AGCTGGAGGG ACCCGTCTAT 
GAGAAGTGCA TGAGCAACAC TGAGTACTGT GAAAGTCCGT ACAGTGAACC TGGAGCGTTT 
GAGGAGGGGG AGCAGGATGG AGGGACGGAA ACACAGACGA GTGAGGAGGA GGGTGAGCCT 
GTGGAGGCGA TAGCCAAGTT TGACTATGTG GGCCGTTCGG GACGGGAGCT CACCTTTAAG 
AAAGGCTCCA CTCTGCTGCT CTTCCAGAGG GCTTCTGAAG ACTGGTGGGA GGGACGACAC 
AACGGCACCG ATGGACTCAT ACCTCACCAA TACGTCACGC TGCAGGAGGA TGAGGCCATG 
TCAGACACTC TGAGCCAGAA AACAGACAGT GAGGCCAGCA CTACTCCTTC AGATGAAGTG 
AGCTCCAGAC GCTCACTGAC GTCTCCTACT GAACCCAGAA TACCTGAGAC CTTCATCAGC 
CGACACCGGA AGCGGACTGA AGCTCTCCTG CGCCGTGTCC CAGGACGTCT GAGCGATAAT 
CACTGCCATG GGAATGGACT GGAACGCAGT TCTCCTCCGG TAACGGTGAC GGTAACAGGT 
CATTTCAGCC CGCGGGAGCT GCTGCGGGGG AACAGCTGTG CGCTGGACAG TCCTGAGCAC 
CACAGACGCC CGGGACTCAA TGGTTCCTCC AATGGGAACA TCACCTCCAG TATCAGCCGA 
CACGAGTCCC TGCGCAGAGA GCGAGAGAGT CCACCGATAC GCCAGTCTCC ATCTGCTCTG 
CACTGCGGGT TACCCATTCA ACGCTCTCAG ACGCTCAACC CGCATACAAT GGCACAGGAC 
ATAGAGGAGA CCATGAGTCT GGCCCTGAAC GAGCTGCGTG AGCTGGAAAG GCAGAACGGC 
AAAACCGCAG CTCCTGACCT GGTGCTGGAC ACCCTGGAGC AGGTGAAAAG CAGCCCGTCC 
TGCCCATCAG GCTCCACACC CTCCAGCCTG TCCAGTCCGT CCGACTCCCC GAGCCCCTTG 
AGCCCCATCA GCCCCCTCCC TCCTCTCCCT CCACTTCCCG GCCCTTCGTC CCGCCGCACA 
AATGACCTGG TGGCCACCTT CAAACCCATC GCCTCACCCC GTATGGGTGT AACCCTCCGC 
CCACCAGCTT TACGCCCTAA ACCGCCCATT CTCCCCAAGG CCAACCCTCC CCCTTCCCAG 
CAGCCCCAAA ATCCAGACAA ATCCGGCACT ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164640.1
CDS	484..3699
Translation	MSNSTKSKKEKEIITEYESQVKEIRSQLVEQQKCLEQQTDMRVQLLQDLQDFFRKKAEIESEYSRNLEKLAERFMAKTRSTKDHQQYKKDQNLLSPVNCWYLLLNQVRRESKDHATLSDLYLNNVITRFTHVSEDNMRLLKKSKEIIFQLQEDLMKILNELYTVMKTYHMYHTETLSAENKLKEAERQGERQGRGGEAVFSLRTEDRHQRRNAARKIQKMKEKRQAKYSENKLKVMKARNEYLLTLEATNASLFKYYIHDLSHLTDCCDLGYHAGLSRALRTYLSAEYSLETSRHEGLDILEGAVEGLDPGNDRQRFMETHPTSFTPPTRFTFQPHMGDQVSQITALPQVQAELLLRFQQLQSRLSTLKIENEEVKKTSEATLSTIQDMVNMEDYDVSESFQHSRSTESVKSSVSDTYLSKPSLAKRRANQQETELFYFTKLKEYLEGSNLIAKLQAKHDMLKRSIAEGYRADIMTTSRRRNSHSRNQDSGKAIPLIVESCIRFINLYGLQHQGIFRVSGSQVEVNDIKNSFERGNDPLTDEENNHDINSVAGVLKMYFRNLDNPIFPKEKFNDLIACVRTESLYERALSIRKILTTMPRPTLVLMRYLFAFLNHLSQYSDENMMDPGNLAICFGPTLMPTPDLLDQVSCQAHVNEIVKTIIIHHETIFPDTKELEGPVYEKCMSNTEYCESPYSEPGAFEEGEQDGGTETQTSEEEGEPVEAIAKFDYVGRSGRELTFKKGSTLLLFQRASEDWWEGRHNGTDGLIPHQYVTLQEDEAMSDTLSQKTDSEASTTPSDEVSSRRSLTSPTEPRIPETFISRHRKRTEALLRRVPGRLSDNHCHGNGLERSSPPVTVTVTGHFSPRELLRGNSCALDSPEHHRRPGLNGSSNGNITSSISRHESLRRERESPPIRQSPSALHCGLPIQRSQTLNPHTMAQDIEETMSLALNELRELERQNGKTAAPDLVLDTLEQVKSSPSCPSGSTPSSLSSPSDSPSPLSPISPLPPLPPLPGPSSRRTNDLVATFKPIASPRMGVTLRPPALRPKPPILPKANPPPSQQPQNPDKSGTM

Target ORF information:

RefSeq Version	XM_005164583.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SLIT-ROBO Rho GTPase activating protein 1b (srgap1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164583.4

ORF Insert Sequence:

ATGTCTAACT CGACGAAGAG CAAAAAGGAA AAGGAAATCA TTACGGAGTA TGAGAGCCAA 
GTGAAAGAGA TTCGGTCTCA GCTGGTGGAG CAGCAGAAAT GTCTGGAGCA GCAGACGGAC 
ATGCGGGTTC AGCTCCTCCA GGACTTGCAG GATTTCTTCA GGAAGAAAGC CGAGATCGAG 
AGCGAATATT CACGCAACCT GGAGAAGCTC GCCGAACGCT TTATGGCCAA AACCCGCAGC 
ACCAAGGACC ACCAACAGTA CAAGAAGGAC CAGAATCTGC TGTCTCCAGT GAACTGCTGG 
TATCTGCTGC TGAACCAGGT GAGGAGGGAG AGTAAAGACC ACGCCACACT GAGTGACCTT 
TACCTCAACA ACGTCATCAC TCGCTTCACA CATGTCAGCG AGGACAACAT GCGTCTGCTC 
AAGAAGAGCA AAGAGATCAT TTTCCAGCTG CAAGAAGACC TGATGAAGAT CCTTAACGAA 
CTCTACACGG TGATGAAGAC GTATCACATG TATCACACCG AGACTCTGAG TGCCGAGAAT 
AAGCTAAAAG AGGCGGAGAG GCAGGGTGAG CGACAGGGCC GAGGCGGAGA GGCCGTCTTC 
AGTCTGCGCA CCGAAGACCG CCACCAACGG CGCAACGCGG CCCGCAAGAT CCAGAAGATG 
AAGGAGAAGC GCCAAGCCAA GTACTCGGAG AACAAACTGA AGGTTATGAA AGCACGGAAC 
GAGTATCTGT TGACCCTGGA GGCCACGAAC GCTTCTCTAT TTAAATATTA CATCCACGAC 
CTGTCGCATC TGACTGACTG TTGTGATCTC GGCTATCATG CGGGCTTGAG TCGGGCATTG 
CGGACCTACC TATCAGCCGA GTACAGTTTG GAAACATCTC GACATGAGGG CCTGGATATT 
TTGGAGGGGG CCGTGGAAGG GCTGGACCCC GGTAATGACC GGCAGCGATT TATGGAGACT 
CACCCAACAT CCTTCACTCC TCCTACACGC TTTACCTTTC AGCCACACAT GGGAGACCAG 
GTGAGTCAAA TCACAGCACT GCCGCAGGTG CAGGCCGAAC TGCTCCTCCG ATTCCAGCAG 
CTGCAGTCGC GCCTCTCCAC ACTCAAGATC GAAAATGAGG AGGTGAAAAA GACATCAGAG 
GCCACACTCA GCACCATCCA GGACATGGTG AACATGGAGG ACTACGACGT TTCTGAGAGT 
TTCCAGCACA GTCGCTCCAC TGAGTCGGTC AAATCCAGCG TATCAGACAC TTACCTCAGC 
AAACCCAGCC TGGCCAAGAG ACGCGCCAAC CAGCAGGAAA CAGAGCTCTT CTACTTCACC 
AAACTGAAGG AGTATCTGGA AGGCAGTAAT CTCATAGCTA AACTGCAAGC CAAGCATGAC 
ATGCTGAAGA GGAGCATAGC TGAAGGATAC AGGGCAGACA TCATGACAAC TAGCCGTCGA 
CGAAACTCTC ACAGCAGAAA CCAGGATTCT GGGAAAGCCA TTCCTCTGAT TGTAGAAAGC 
TGCATTCGCT TTATTAACCT ATATGGTCTG CAACACCAGG GTATTTTCCG GGTTTCAGGG 
TCACAAGTGG AGGTCAATGA CATCAAAAAC TCTTTTGAAC GAGGTAATGA TCCGCTGACT 
GATGAGGAGA ATAATCATGA CATTAACTCA GTGGCAGGCG TCCTAAAAAT GTACTTCCGC 
AACCTGGACA ATCCAATTTT CCCAAAGGAG AAGTTTAACG ATCTGATCGC CTGCGTTCGA 
ACTGAGAGTT TGTATGAGCG TGCCCTCTCC ATCCGCAAGA TCCTCACCAC AATGCCACGG 
CCAACACTGG TGCTTATGCG ATACCTGTTT GCATTCCTCA ACCATTTGTC CCAGTACAGT 
GACGAGAACA TGATGGACCC TGGAAACCTG GCTATTTGCT TTGGACCCAC TTTAATGCCC 
ACTCCGGACC TCCTAGACCA GGTCTCCTGT CAGGCCCATG TCAACGAGAT TGTCAAAACC 
ATCATCATCC ACCATGAAAC CATCTTCCCT GACACCAAAG AGCTGGAGGG ACCCGTCTAT 
GAGAAGTGCA TGAGCAACAC TGAGTACTGT GAAAGTCCGT ACAGTGAACC TGGAGCGTTT 
GAGGAGGGGG AGCAGGATGG AGGGACGGAA ACACAGACGA GTGAGGAGGA GGGTGAGCCT 
GTGGAGGCGA TAGCCAAGTT TGACTATGTG GGCCGTTCGG GACGGGAGCT CACCTTTAAG 
AAAGGCTCCA CTCTGCTGCT CTTCCAGAGG GCTTCTGAAG ACTGGTGGGA GGGACGACAC 
AACGGCACCG ATGGACTCAT ACCTCACCAA TACGTCACGC TGCAGGAGGA TGAGGCCATG 
TCAGACACTC TGAGCCAGAA AACAGACAGT GAGGCCAGCA CTACTCCTTC AGATGAAGTG 
AGCTCCAGAC GCTCACTGAC GTCTCCTACT GAACCCAGAA TACCTGAGAC CTTCATCAGC 
CGACACCGGA AGCGGACTGA AGCTCTCCTG CGCCGTGTCC CAGGACGTCT GAGCGATAAT 
CACTGCCATG GGAATGGACT GGAACGCAGT TCTCCTCCGG TAACGGTGAC GGTAACAGGT 
CATTTCAGCC CGCGGGAGCT GCTGCGGGGG AACAGCTGTG CGCTGGACAG TCCTGAGCAC 
CACAGACGCC CGGGACTCAA TGGTTCCTCC AATGGGAACA TCACCTCCAG TATCAGCCGA 
CACGAGTCCC TGCGCAGAGA GCGAGAGAGT CCACCGATAC GCCAGTCTCC ATCTGCTCTG 
CACTGCGGGT TACCCATTCA ACGCTCTCAG ACGCTCAACC CGCATACAAT GGCACAGGAC 
ATAGAGGAGA CCATGAGTCT GGCCCTGAAC GAGCTGCGTG AGCTGGAAAG GCAGAACGGC 
AAAACCGCAG CTCCTGACCT GGTGCTGGAC ACCCTGGAGC AGGTGAAAAG CAGCCCGTCC 
TGCCCATCAG GCTCCACACC CTCCAGCCTG TCCAGTCCGT CCGACTCCCC GAGCCCCTTG 
AGCCCCATCA GCCCCCTCCC TCCTCTCCCT CCACTTCCCG GCCCTTCGTC CCGCCGCACA 
AATGACCTGG TGGCCACCTT CAAACCCATC GCCTCACCCC GTATGGGTGT AACCCTCCGC 
CCACCAGCTT TACGCCCTAA ACCGCCCATT CTCCCCAAGG CCAACCCTCC CCCTTCCCAG 
CAGCCCCAAA ATCCAGACAA ATCCGGCACT ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SRGAP1B ( XM_005164583.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005164640.1 Danio rerio SLIT-ROBO Rho GTPase activating protein 1b (srgap1b), transcript variant X1, mRNA.

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CloneID	ODa09197
Clone ID Related Accession (Same CDS sequence)	XM_005164583.4 , XM_005164583.3
Accession Version	XM_005164583.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	SLIT-ROBO Rho GTPase-activating protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005164583.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAACT CGACGAAGAG CAAAAAGGAA AAGGAAATCA TTACGGAGTA TGAGAGCCAA 
GTGAAAGAGA TTCGGTCTCA GCTGGTGGAG CAGCAGAAAT GTCTGGAGCA GCAGACGGAC 
ATGCGGGTTC AGCTCCTCCA GGACTTGCAG GATTTCTTCA GGAAGAAAGC CGAGATCGAG 
AGCGAATATT CACGCAACCT GGAGAAGCTC GCCGAACGCT TTATGGCCAA AACCCGCAGC 
ACCAAGGACC ACCAACAGTA CAAGAAGGAC CAGAATCTGC TGTCTCCAGT GAACTGCTGG 
TATCTGCTGC TGAACCAGGT GAGGAGGGAG AGTAAAGACC ACGCCACACT GAGTGACCTT 
TACCTCAACA ACGTCATCAC TCGCTTCACA CATGTCAGCG AGGACAACAT GCGTCTGCTC 
AAGAAGAGCA AAGAGATCAT TTTCCAGCTG CAAGAAGACC TGATGAAGAT CCTTAACGAA 
CTCTACACGG TGATGAAGAC GTATCACATG TATCACACCG AGACTCTGAG TGCCGAGAAT 
AAGCTAAAAG AGGCGGAGAG GCAGGGTGAG CGACAGGGCC GAGGCGGAGA GGCCGTCTTC 
AGTCTGCGCA CCGAAGACCG CCACCAACGG CGCAACGCGG CCCGCAAGAT CCAGAAGATG 
AAGGAGAAGC GCCAAGCCAA GTACTCGGAG AACAAACTGA AGGTTATGAA AGCACGGAAC 
GAGTATCTGT TGACCCTGGA GGCCACGAAC GCTTCTCTAT TTAAATATTA CATCCACGAC 
CTGTCGCATC TGACTGACTG TTGTGATCTC GGCTATCATG CGGGCTTGAG TCGGGCATTG 
CGGACCTACC TATCAGCCGA GTACAGTTTG GAAACATCTC GACATGAGGG CCTGGATATT 
TTGGAGGGGG CCGTGGAAGG GCTGGACCCC GGTAATGACC GGCAGCGATT TATGGAGACT 
CACCCAACAT CCTTCACTCC TCCTACACGC TTTACCTTTC AGCCACACAT GGGAGACCAG 
GTGAGTCAAA TCACAGCACT GCCGCAGGTG CAGGCCGAAC TGCTCCTCCG ATTCCAGCAG 
CTGCAGTCGC GCCTCTCCAC ACTCAAGATC GAAAATGAGG AGGTGAAAAA GACATCAGAG 
GCCACACTCA GCACCATCCA GGACATGGTG AACATGGAGG ACTACGACGT TTCTGAGAGT 
TTCCAGCACA GTCGCTCCAC TGAGTCGGTC AAATCCAGCG TATCAGACAC TTACCTCAGC 
AAACCCAGCC TGGCCAAGAG ACGCGCCAAC CAGCAGGAAA CAGAGCTCTT CTACTTCACC 
AAACTGAAGG AGTATCTGGA AGGCAGTAAT CTCATAGCTA AACTGCAAGC CAAGCATGAC 
ATGCTGAAGA GGAGCATAGC TGAAGGATAC AGGGCAGACA TCATGACAAC TAGCCGTCGA 
CGAAACTCTC ACAGCAGAAA CCAGGATTCT GGGAAAGCCA TTCCTCTGAT TGTAGAAAGC 
TGCATTCGCT TTATTAACCT ATATGGTCTG CAACACCAGG GTATTTTCCG GGTTTCAGGG 
TCACAAGTGG AGGTCAATGA CATCAAAAAC TCTTTTGAAC GAGGTAATGA TCCGCTGACT 
GATGAGGAGA ATAATCATGA CATTAACTCA GTGGCAGGCG TCCTAAAAAT GTACTTCCGC 
AACCTGGACA ATCCAATTTT CCCAAAGGAG AAGTTTAACG ATCTGATCGC CTGCGTTCGA 
ACTGAGAGTT TGTATGAGCG TGCCCTCTCC ATCCGCAAGA TCCTCACCAC AATGCCACGG 
CCAACACTGG TGCTTATGCG ATACCTGTTT GCATTCCTCA ACCATTTGTC CCAGTACAGT 
GACGAGAACA TGATGGACCC TGGAAACCTG GCTATTTGCT TTGGACCCAC TTTAATGCCC 
ACTCCGGACC TCCTAGACCA GGTCTCCTGT CAGGCCCATG TCAACGAGAT TGTCAAAACC 
ATCATCATCC ACCATGAAAC CATCTTCCCT GACACCAAAG AGCTGGAGGG ACCCGTCTAT 
GAGAAGTGCA TGAGCAACAC TGAGTACTGT GAAAGTCCGT ACAGTGAACC TGGAGCGTTT 
GAGGAGGGGG AGCAGGATGG AGGGACGGAA ACACAGACGA GTGAGGAGGA GGGTGAGCCT 
GTGGAGGCGA TAGCCAAGTT TGACTATGTG GGCCGTTCGG GACGGGAGCT CACCTTTAAG 
AAAGGCTCCA CTCTGCTGCT CTTCCAGAGG GCTTCTGAAG ACTGGTGGGA GGGACGACAC 
AACGGCACCG ATGGACTCAT ACCTCACCAA TACGTCACGC TGCAGGAGGA TGAGGCCATG 
TCAGACACTC TGAGCCAGAA AACAGACAGT GAGGCCAGCA CTACTCCTTC AGATGAAGTG 
AGCTCCAGAC GCTCACTGAC GTCTCCTACT GAACCCAGAA TACCTGAGAC CTTCATCAGC 
CGACACCGGA AGCGGACTGA AGCTCTCCTG CGCCGTGTCC CAGGACGTCT GAGCGATAAT 
CACTGCCATG GGAATGGACT GGAACGCAGT TCTCCTCCGG TAACGGTGAC GGTAACAGGT 
CATTTCAGCC CGCGGGAGCT GCTGCGGGGG AACAGCTGTG CGCTGGACAG TCCTGAGCAC 
CACAGACGCC CGGGACTCAA TGGTTCCTCC AATGGGAACA TCACCTCCAG TATCAGCCGA 
CACGAGTCCC TGCGCAGAGA GCGAGAGAGT CCACCGATAC GCCAGTCTCC ATCTGCTCTG 
CACTGCGGGT TACCCATTCA ACGCTCTCAG ACGCTCAACC CGCATACAAT GGCACAGGAC 
ATAGAGGAGA CCATGAGTCT GGCCCTGAAC GAGCTGCGTG AGCTGGAAAG GCAGAACGGC 
AAAACCGCAG CTCCTGACCT GGTGCTGGAC ACCCTGGAGC AGGTGAAAAG CAGCCCGTCC 
TGCCCATCAG GCTCCACACC CTCCAGCCTG TCCAGTCCGT CCGACTCCCC GAGCCCCTTG 
AGCCCCATCA GCCCCCTCCC TCCTCTCCCT CCACTTCCCG GCCCTTCGTC CCGCCGCACA 
AATGACCTGG TGGCCACCTT CAAACCCATC GCCTCACCCC GTATGGGTGT AACCCTCCGC 
CCACCAGCTT TACGCCCTAA ACCGCCCATT CTCCCCAAGG CCAACCCTCC CCCTTCCCAG 
CAGCCCCAAA ATCCAGACAA ATCCGGCACT ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164640.1
CDS	558..3773
Translation	MSNSTKSKKEKEIITEYESQVKEIRSQLVEQQKCLEQQTDMRVQLLQDLQDFFRKKAEIESEYSRNLEKLAERFMAKTRSTKDHQQYKKDQNLLSPVNCWYLLLNQVRRESKDHATLSDLYLNNVITRFTHVSEDNMRLLKKSKEIIFQLQEDLMKILNELYTVMKTYHMYHTETLSAENKLKEAERQGERQGRGGEAVFSLRTEDRHQRRNAARKIQKMKEKRQAKYSENKLKVMKARNEYLLTLEATNASLFKYYIHDLSHLTDCCDLGYHAGLSRALRTYLSAEYSLETSRHEGLDILEGAVEGLDPGNDRQRFMETHPTSFTPPTRFTFQPHMGDQVSQITALPQVQAELLLRFQQLQSRLSTLKIENEEVKKTSEATLSTIQDMVNMEDYDVSESFQHSRSTESVKSSVSDTYLSKPSLAKRRANQQETELFYFTKLKEYLEGSNLIAKLQAKHDMLKRSIAEGYRADIMTTSRRRNSHSRNQDSGKAIPLIVESCIRFINLYGLQHQGIFRVSGSQVEVNDIKNSFERGNDPLTDEENNHDINSVAGVLKMYFRNLDNPIFPKEKFNDLIACVRTESLYERALSIRKILTTMPRPTLVLMRYLFAFLNHLSQYSDENMMDPGNLAICFGPTLMPTPDLLDQVSCQAHVNEIVKTIIIHHETIFPDTKELEGPVYEKCMSNTEYCESPYSEPGAFEEGEQDGGTETQTSEEEGEPVEAIAKFDYVGRSGRELTFKKGSTLLLFQRASEDWWEGRHNGTDGLIPHQYVTLQEDEAMSDTLSQKTDSEASTTPSDEVSSRRSLTSPTEPRIPETFISRHRKRTEALLRRVPGRLSDNHCHGNGLERSSPPVTVTVTGHFSPRELLRGNSCALDSPEHHRRPGLNGSSNGNITSSISRHESLRRERESPPIRQSPSALHCGLPIQRSQTLNPHTMAQDIEETMSLALNELRELERQNGKTAAPDLVLDTLEQVKSSPSCPSGSTPSSLSSPSDSPSPLSPISPLPPLPPLPGPSSRRTNDLVATFKPIASPRMGVTLRPPALRPKPPILPKANPPPSQQPQNPDKSGTM

Target ORF information:

RefSeq Version	XM_005164583.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SLIT-ROBO Rho GTPase activating protein 1b (srgap1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164583.3

ORF Insert Sequence:

ATGTCTAACT CGACGAAGAG CAAAAAGGAA AAGGAAATCA TTACGGAGTA TGAGAGCCAA 
GTGAAAGAGA TTCGGTCTCA GCTGGTGGAG CAGCAGAAAT GTCTGGAGCA GCAGACGGAC 
ATGCGGGTTC AGCTCCTCCA GGACTTGCAG GATTTCTTCA GGAAGAAAGC CGAGATCGAG 
AGCGAATATT CACGCAACCT GGAGAAGCTC GCCGAACGCT TTATGGCCAA AACCCGCAGC 
ACCAAGGACC ACCAACAGTA CAAGAAGGAC CAGAATCTGC TGTCTCCAGT GAACTGCTGG 
TATCTGCTGC TGAACCAGGT GAGGAGGGAG AGTAAAGACC ACGCCACACT GAGTGACCTT 
TACCTCAACA ACGTCATCAC TCGCTTCACA CATGTCAGCG AGGACAACAT GCGTCTGCTC 
AAGAAGAGCA AAGAGATCAT TTTCCAGCTG CAAGAAGACC TGATGAAGAT CCTTAACGAA 
CTCTACACGG TGATGAAGAC GTATCACATG TATCACACCG AGACTCTGAG TGCCGAGAAT 
AAGCTAAAAG AGGCGGAGAG GCAGGGTGAG CGACAGGGCC GAGGCGGAGA GGCCGTCTTC 
AGTCTGCGCA CCGAAGACCG CCACCAACGG CGCAACGCGG CCCGCAAGAT CCAGAAGATG 
AAGGAGAAGC GCCAAGCCAA GTACTCGGAG AACAAACTGA AGGTTATGAA AGCACGGAAC 
GAGTATCTGT TGACCCTGGA GGCCACGAAC GCTTCTCTAT TTAAATATTA CATCCACGAC 
CTGTCGCATC TGACTGACTG TTGTGATCTC GGCTATCATG CGGGCTTGAG TCGGGCATTG 
CGGACCTACC TATCAGCCGA GTACAGTTTG GAAACATCTC GACATGAGGG CCTGGATATT 
TTGGAGGGGG CCGTGGAAGG GCTGGACCCC GGTAATGACC GGCAGCGATT TATGGAGACT 
CACCCAACAT CCTTCACTCC TCCTACACGC TTTACCTTTC AGCCACACAT GGGAGACCAG 
GTGAGTCAAA TCACAGCACT GCCGCAGGTG CAGGCCGAAC TGCTCCTCCG ATTCCAGCAG 
CTGCAGTCGC GCCTCTCCAC ACTCAAGATC GAAAATGAGG AGGTGAAAAA GACATCAGAG 
GCCACACTCA GCACCATCCA GGACATGGTG AACATGGAGG ACTACGACGT TTCTGAGAGT 
TTCCAGCACA GTCGCTCCAC TGAGTCGGTC AAATCCAGCG TATCAGACAC TTACCTCAGC 
AAACCCAGCC TGGCCAAGAG ACGCGCCAAC CAGCAGGAAA CAGAGCTCTT CTACTTCACC 
AAACTGAAGG AGTATCTGGA AGGCAGTAAT CTCATAGCTA AACTGCAAGC CAAGCATGAC 
ATGCTGAAGA GGAGCATAGC TGAAGGATAC AGGGCAGACA TCATGACAAC TAGCCGTCGA 
CGAAACTCTC ACAGCAGAAA CCAGGATTCT GGGAAAGCCA TTCCTCTGAT TGTAGAAAGC 
TGCATTCGCT TTATTAACCT ATATGGTCTG CAACACCAGG GTATTTTCCG GGTTTCAGGG 
TCACAAGTGG AGGTCAATGA CATCAAAAAC TCTTTTGAAC GAGGTAATGA TCCGCTGACT 
GATGAGGAGA ATAATCATGA CATTAACTCA GTGGCAGGCG TCCTAAAAAT GTACTTCCGC 
AACCTGGACA ATCCAATTTT CCCAAAGGAG AAGTTTAACG ATCTGATCGC CTGCGTTCGA 
ACTGAGAGTT TGTATGAGCG TGCCCTCTCC ATCCGCAAGA TCCTCACCAC AATGCCACGG 
CCAACACTGG TGCTTATGCG ATACCTGTTT GCATTCCTCA ACCATTTGTC CCAGTACAGT 
GACGAGAACA TGATGGACCC TGGAAACCTG GCTATTTGCT TTGGACCCAC TTTAATGCCC 
ACTCCGGACC TCCTAGACCA GGTCTCCTGT CAGGCCCATG TCAACGAGAT TGTCAAAACC 
ATCATCATCC ACCATGAAAC CATCTTCCCT GACACCAAAG AGCTGGAGGG ACCCGTCTAT 
GAGAAGTGCA TGAGCAACAC TGAGTACTGT GAAAGTCCGT ACAGTGAACC TGGAGCGTTT 
GAGGAGGGGG AGCAGGATGG AGGGACGGAA ACACAGACGA GTGAGGAGGA GGGTGAGCCT 
GTGGAGGCGA TAGCCAAGTT TGACTATGTG GGCCGTTCGG GACGGGAGCT CACCTTTAAG 
AAAGGCTCCA CTCTGCTGCT CTTCCAGAGG GCTTCTGAAG ACTGGTGGGA GGGACGACAC 
AACGGCACCG ATGGACTCAT ACCTCACCAA TACGTCACGC TGCAGGAGGA TGAGGCCATG 
TCAGACACTC TGAGCCAGAA AACAGACAGT GAGGCCAGCA CTACTCCTTC AGATGAAGTG 
AGCTCCAGAC GCTCACTGAC GTCTCCTACT GAACCCAGAA TACCTGAGAC CTTCATCAGC 
CGACACCGGA AGCGGACTGA AGCTCTCCTG CGCCGTGTCC CAGGACGTCT GAGCGATAAT 
CACTGCCATG GGAATGGACT GGAACGCAGT TCTCCTCCGG TAACGGTGAC GGTAACAGGT 
CATTTCAGCC CGCGGGAGCT GCTGCGGGGG AACAGCTGTG CGCTGGACAG TCCTGAGCAC 
CACAGACGCC CGGGACTCAA TGGTTCCTCC AATGGGAACA TCACCTCCAG TATCAGCCGA 
CACGAGTCCC TGCGCAGAGA GCGAGAGAGT CCACCGATAC GCCAGTCTCC ATCTGCTCTG 
CACTGCGGGT TACCCATTCA ACGCTCTCAG ACGCTCAACC CGCATACAAT GGCACAGGAC 
ATAGAGGAGA CCATGAGTCT GGCCCTGAAC GAGCTGCGTG AGCTGGAAAG GCAGAACGGC 
AAAACCGCAG CTCCTGACCT GGTGCTGGAC ACCCTGGAGC AGGTGAAAAG CAGCCCGTCC 
TGCCCATCAG GCTCCACACC CTCCAGCCTG TCCAGTCCGT CCGACTCCCC GAGCCCCTTG 
AGCCCCATCA GCCCCCTCCC TCCTCTCCCT CCACTTCCCG GCCCTTCGTC CCGCCGCACA 
AATGACCTGG TGGCCACCTT CAAACCCATC GCCTCACCCC GTATGGGTGT AACCCTCCGC 
CCACCAGCTT TACGCCCTAA ACCGCCCATT CTCCCCAAGG CCAACCCTCC CCCTTCCCAG 
CAGCCCCAAA ATCCAGACAA ATCCGGCACT ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dissection of Glomerular Transcriptional Profile in Patients With Diabetic Nephropathy: SRGAP2a Protects Podocyte Structure and Function.
Diabetes67(4)717-730(2018 04)
Pan Y,Jiang S,Hou Q,Qiu D,Shi J,Wang L,Chen Z,Zhang M,Duan A,Qin W,Zen K,Liu Z

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Eumetazoan cryptochrome phylogeny and evolution.
Genome biology and evolution7(2)601-19(2015 Jan)
Haug MF,Gesemann M,Lazović V,Neuhauss SC

On the crucial cerebellar wound healing-related pathways and their cross-talks after traumatic brain injury in Danio rerio.
PloS one9(6)e97902(2014)
Wu CC,Tsai TH,Chang C,Lee TT,Lin C,Cheng IH,Sun MC,Chuang YJ,Chen BS

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SRGAP1B cDNA ORF clone, Danio rerio(Zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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