gnptab cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	gnptab
Entrez Gene ID	553365
Full Name	N-acetylglucosamine-1-phosphate transferase, alpha and beta subunits
Synonyms	si:ch211-234f20.3,wu:fd55d09,zgc:122985,zgc:158502
General protein information	Preferred Names N-acetylglucosamine-1-phosphate transferase, alpha and beta subunits Names N-acetylglucosamine-1-phosphotransferase subunits alpha/beta UDP-N-acetylglucosamine-1-phosphotransferase subunits alpha/beta glcNAc-1-phosphotransferase subunits alpha/beta stealth protein gnptab
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001044768.3	NP_001038233.1	N-acetylglucosamine-1-phosphotransferase subunits alpha/beta
XM_005164744.3	XP_005164801.1	N-acetylglucosamine-1-phosphotransferase subunits alpha/beta isoform X1
XM_005164744.4	XP_005164801.1	N-acetylglucosamine-1-phosphotransferase subunits alpha/beta isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	GNPTAB	NP_001179157.1
Xenopus tropicalis (tropical clawed frog)	gnptab	NP_001090704.1
Homo sapiens (human)	GNPTAB	NP_077288.2
Rattus norvegicus (Norway rat)	Gnptab	XP_343195.3
Pan troglodytes (chimpanzee)	GNPTAB	XP_001155334.2
Canis lupus familiaris (dog)	GNPTAB	XP_005629499.1
Gallus gallus (chicken)	GNPTAB	XP_416329.2
Macaca mulatta (Rhesus monkey)	GNPTAB	XP_001092998.1
Mus musculus (house mouse)	Gnptab	NP_001004164.2
Danio rerio (zebrafish)	gnptab	NP_001038233.1

Gene Ontology
Bibliography

Related articles in PubMed

Enzyme-specific differences in mannose phosphorylation between GlcNAc-1-phosphotransferase αβ and γ subunit deficient zebrafish support cathepsin proteases as early mediators of mucolipidosis pathology.
Flanagan-Steet H, Matheny C, Petrey A, Parker J, Steet R
Biochimica et biophysica acta1860(9)1845-53(2016 09)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Cathepsin-Mediated Alterations in TGFß-Related Signaling Underlie Disrupted Cartilage and Bone Maturation Associated With Impaired Lysosomal Targeting.
Flanagan-Steet H, Aarnio M, Kwan B, Guihard P, Petrey A, Haskins M, Blanchard F, Steet R
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research31(3)535-48(2016 Mar)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Analysis of mucolipidosis II/III GNPTAB missense mutations identifies domains of UDP-GlcNAc:lysosomal enzyme GlcNAc-1-phosphotransferase involved in catalytic function and lysosomal enzyme recognition.
Qian Y, van Meel E, Flanagan-Steet H, Yox A, Steet R, Kornfeld S
The Journal of biological chemistry290(5)3045-56(2015 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following gnptab gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gnptab cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa01224	NM_001044768.3 Latest version!	Danio rerio N-acetylglucosamine-1-phosphate transferase subunits alpha and beta (gnptab), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa09372	XM_005164744.4 Latest version!	Danio rerio N-acetylglucosamine-1-phosphate transferase, alpha and beta subunits (gnptab), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
ODa09372	XM_005164744.3	Danio rerio N-acetylglucosamine-1-phosphate transferase, alpha and beta subunits (gnptab), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gnptab ( NM_001044768.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001038233.1 Danio rerio N-acetylglucosamine-1-phosphate transferase subunits alpha and beta (gnptab), mRNA.

Price & Availability↑

CloneID	ODa01224
Clone ID Related Accession (Same CDS sequence)	NM_001044768.3
Accession Version	NM_001044768.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1551110400000
Organism	Danio rerio(zebrafish)
Product	N-acetylglucosamine-1-phosphotransferase subunits alpha/beta
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX890572.9. On or before Dec 20, 2008 this sequence version replaced NM_001083021.1, NM_001044768.2. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2168447, SAMEA2168448 [ECO:0000348] ##Evidence-Data-END##

ATGTTGGTCG TTAACTCGCT GTTGAAGCTG CTGCAGCGGC AAACCTACAC CTGCCTGTCC 
CACAGATACG GGCTTTACCT GTGTTTCGGA GGACTGGTGC TCATGATCGT CTCCGCCTTT 
CAATTTGGAG AGGTTGTAGT TGAATGGAGT CGAGATCAGT ACCATGTTTT GTTTGATTCC 
TACAGAGATA ATGTTGCTGG AAAGTCTTTT CAGACGAGGC TTTGCCTCCC GATGCCCATC 
GATGTGGTGT ACACATGGGT GAATGGCACA GACATAAACC TGCTGAAGGA TCTTCGTGCT 
GTCAGACAGC GACTGGAAGA AGAACAGAAG GCCTTGAGGG AGCGTCTGGG AAAAAATGGC 
TCTGAAATAA CAGAAGCTCC TAAAGGGCGG CCAGAATGTC TACTGTCGCA CTGCATTATG 
GGTCCAGTGC TGGTACTGGA TCCTGCTCTC CCTGCCAACA TCACAGTGAA GGAGCTGCCC 
ACACTTTCAG CCGCCTTCTC TTCTGCTAAA GAGCTCCTGC AGGTGGCCAA ACCTCTGCAT 
CCGTCCAGCA GTGTCACTGC GCTCCTCTTC CACTCTCACA CTGAAGCTGA AAAAGCTCAC 
GCAGACGCTT TGAAGGATCT CCTGACACAC TCCATCAGCA GAGGTTACCT GACGACTGAT 
AAAGAGGCTC CGGGATTGGT CCGCATGCAC ACTCTGGCCT ACTTGAGCGG CTTTCCAGCA 
TCGCTGAAGG AAACTGAGCA GCTCCGGGTC AAACTGCCAG CTGTAGTGAC CAGTAAAACC 
AAAAAGTTGC AGTTATACTC TGAAGCCAGC ATCGCTCTTC TCCACCTGAA CACAGCACAG 
GATTTCACAG ATCTCACACA GCAGGCCAAG AAGAACCTGA CACTGGATGG GAAAGAGCTG 
ACAGTCAGCT CCGCGTTTCT GTTCTGGGAC CTCACTGCCA TCTCACAGTC TAAGCAGGAT 
GAGGATGTTT CTGCCAGTCG CTTTGAAGAC AATGAAGAGC TGCGCTATTC ACTGCGCTCC 
ATCGAAAAGC ACGCGCCCTG GGTGCGGCAC ATCTTTATCG TAACAAACGG ACAGATACCG 
TCTTGGCTTA ACCTGGATAA TCCACGGGTT TCAGTGGTTA CTCACCAGGA TATTTTCCAG 
AATCAGACTC ATCTGCCTAC GTTCAGTTCT CCTGCCATAG AGACCCACAT CCACCGCATC 
CCAGGACTCT CTCAAAAGTT CATCTACCTC AATGATGATG TCATGTTCGG CAAGGATGTG 
TGGCCTGATG ACTTCTATAG CCACTCCAAA GGCCAAAAGG TGTATCTTAC CTGGCCTGTG 
CCAAATTGTG CTGAGGGCTG TCCAGGATCC TGGATCAAAG ACGGCTACTG CGACAAGGCC 
TGCAACAACT CTGCATGTGA CTGGGATGGA GGAGACTGTC AAGGCAGCAG TCGCTTTGGT 
GGAGCAGGTG GCTCTGTGAT CGGCGGTGGG CAGCCCTGGC AGTTTGCAGG TGGTCTTGGA 
GGTCTGGCTG GCATGTCCTT CTGTAATCAG GGCTGTGCTA ACTCTTGGTT GGCTGATAAG 
TTCTGTGACC AGGCATGCAA TGTTCTGGCC TGTGGGTTTG ATGTGGGCGA CTGCGGACAA 
GACAATTTAA ACCAGCTGCA CCGCATCGTG TTGAGGAGGA ACCAGACTCT GTACACCCTT 
CCTCAAGGAG AGCTCAGACC ATATTTCAGT TTCTCCGGCC TGGCGAATCG TGTGTCCGAG 
GCTCATGTAG CCGATAACCA AGTCCTGCGA CACACTTCAG TTGCCAACAA ATGGAAAACC 
ATCCACTTGC TGCTGCTTCC GGGACACAAC GCCACCCAAA TCCACTACAA CATCACCTTC 
CAGAGCACAG ACCACCACGA CTTCATCATG ACCTTCTCAG TCAGTGTTGA CACCCGAGAG 
CTGCCCAAAT CAAACACCTC CACTCCAGTG CGGGACAAAG AAGAAGAGCC CAAACCAACT 
GTAGCGACCC CAGAACCAGA AGTCCCATTC GAAGCTGTTC CCAAAGAAAA ACAAGGCCCT 
AAAGTTAGGG AACAAACACA CGGTGATGTA CAGGTGCCTG TGCTAAATGA GACTCTCTTG 
CCTGACGAAG TGAAGATCGA GTTGAAAAAG CTGAACGAGA AGCTAATGTC AGGTGACATC 
ACTATTAAAG GCTTTAATTT GACCAAAGCA ATGCTGTTGG AGCCCTATAA AGAGAAAGAT 
CAGTTTACAT TAAAACACGA AAAGGACAAA GAAGAAAAGC AGAAACTTTC TCCCGTGGCT 
CAGCTTCAGA ATGAGAGAGC AGCAGCAAGA GTGAAGAGAG AAAAAGGCGA AAATGTTGAT 
CAACTGAAGC CAGTTGCTCC ATCTCTGGTG CCGGTTCAGA TTGATGATGT CACAACCAAA 
GCTCAGTCAC GGTTGTTCAA CATAGAGAAA CCTCACATGT TTCATCTTCT TAGTAACCTG 
AAGGGGAACT TATCTAAGGA GAGGGATTCA GACTCCGAGG GTCATCTCCG CGAGCGGCCA 
ACTGGTCGTA GGCTTCAGTT TTACAGCGAC AGCTTGAACA GAGGTTTCCT GCCGTGGGAG 
AAGAGGAAGT TCTTTCAGGA TCTGATGGAG GAAGTAAATC GTCTCCAGAC TGAACTGCAG 
TACACAGCTG ACCGCACCGC CACTGGACGC AGACTGCAGG ACACTTTTGC CGATTCACTC 
CGATATGTCA ACCGACTTTT AAATGCTCAA TTTGGCTTCA CCTCCCGCAA AGTGCCTGCG 
CACATGCCTC ACATGATAGA CAGACTCATC ATGCAGGAAC TACAGGACAC TTTTCCTCAG 
GAGTTTGATA AGACCTCTTC TCACCGTGTG CGCCACTCTG AGGACATGCA GTTCGCTTTT 
TCTTATTTCT ACTTTTTAAT GAGTGCTGTG CAGCAGCTCA ATATATCTGA AGTGTTTGAT 
GAAATCGACA CGGATCACTC TGGTGTGCTG TCTGACCGAG AGATCCGGAC ACTGGCCACA 
CGCATCCATG AGCTGCCCTT AAGCCTTCAG GATTTGACCA GCCTAGAACA GATGCTTATA 
AACTGCTCAA AGTCTCTTCC ATCCAACCTC ACGCACCTCC ATGCTGTCAG TCCCACCCAG 
GAGGCCTATT ACGACCCCAG CATGCCACCA GTCACCAAAG GTTTAGTGAT CCACTGCAAA 
CCCATCACAG AGAGGATCCA CAAAGCCTTC AAAGACCAGA ATAAATACAA GTTTGAAATT 
ATGGGTGAAG AGGAGATCGC CTTTAAGATG ATACGAACCA ACGTATCTCA TGTAGTCGGG 
CAGCTCGATG ATATTAGGAA GAACCCTAGG AAGTTTATTT GCCTGAATGA CAACATTGAC 
CACATTCATA AGGATGCGGG CACGGTAAAA GCGGTACTGA GGGACTTCTA TGAGTCTATG 
TTCCCACTGC CATCTCAGTT TGAGCTGCCC AGAGAATACA GAAACCGCTT CCTCCACATG 
ACTGAACTGC AGGAGTGGCG AATATACAGA GACAAGCTGA AGTTCTGGAC CCACTGTGTG 
CTGGTGACAT TAGTCGTCTT CACTGTCATG TCTTTCTTTG CTGAGCAGCT GGTCATGCTG 
AAGCGATGGC TCTTCCCAAG ACGCAGAGTC AGCAAAGATG CAAACCCTGA GCGGGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001038233.1
CDS	1..3660
Translation	MLVVNSLLKLLQRQTYTCLSHRYGLYLCFGGLVLMIVSAFQFGEVVVEWSRDQYHVLFDSYRDNVAGKSFQTRLCLPMPIDVVYTWVNGTDINLLKDLRAVRQRLEEEQKALRERLGKNGSEITEAPKGRPECLLSHCIMGPVLVLDPALPANITVKELPTLSAAFSSAKELLQVAKPLHPSSSVTALLFHSHTEAEKAHADALKDLLTHSISRGYLTTDKEAPGLVRMHTLAYLSGFPASLKETEQLRVKLPAVVTSKTKKLQLYSEASIALLHLNTAQDFTDLTQQAKKNLTLDGKELTVSSAFLFWDLTAISQSKQDEDVSASRFEDNEELRYSLRSIEKHAPWVRHIFIVTNGQIPSWLNLDNPRVSVVTHQDIFQNQTHLPTFSSPAIETHIHRIPGLSQKFIYLNDDVMFGKDVWPDDFYSHSKGQKVYLTWPVPNCAEGCPGSWIKDGYCDKACNNSACDWDGGDCQGSSRFGGAGGSVIGGGQPWQFAGGLGGLAGMSFCNQGCANSWLADKFCDQACNVLACGFDVGDCGQDNLNQLHRIVLRRNQTLYTLPQGELRPYFSFSGLANRVSEAHVADNQVLRHTSVANKWKTIHLLLLPGHNATQIHYNITFQSTDHHDFIMTFSVSVDTRELPKSNTSTPVRDKEEEPKPTVATPEPEVPFEAVPKEKQGPKVREQTHGDVQVPVLNETLLPDEVKIELKKLNEKLMSGDITIKGFNLTKAMLLEPYKEKDQFTLKHEKDKEEKQKLSPVAQLQNERAAARVKREKGENVDQLKPVAPSLVPVQIDDVTTKAQSRLFNIEKPHMFHLLSNLKGNLSKERDSDSEGHLRERPTGRRLQFYSDSLNRGFLPWEKRKFFQDLMEEVNRLQTELQYTADRTATGRRLQDTFADSLRYVNRLLNAQFGFTSRKVPAHMPHMIDRLIMQELQDTFPQEFDKTSSHRVRHSEDMQFAFSYFYFLMSAVQQLNISEVFDEIDTDHSGVLSDREIRTLATRIHELPLSLQDLTSLEQMLINCSKSLPSNLTHLHAVSPTQEAYYDPSMPPVTKGLVIHCKPITERIHKAFKDQNKYKFEIMGEEEIAFKMIRTNVSHVVGQLDDIRKNPRKFICLNDNIDHIHKDAGTVKAVLRDFYESMFPLPSQFELPREYRNRFLHMTELQEWRIYRDKLKFWTHCVLVTLVVFTVMSFFAEQLVMLKRWLFPRRRVSKDANPERV

Target ORF information:

RefSeq Version	NM_001044768.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio N-acetylglucosamine-1-phosphate transferase subunits alpha and beta (gnptab), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001044768.3

ORF Insert Sequence:

ATGTTGGTCG TTAACTCGCT GTTGAAGCTG CTGCAGCGGC AAACCTACAC CTGCCTGTCC 
CACAGATACG GGCTTTACCT GTGTTTCGGA GGACTGGTGC TCATGATCGT CTCCGCCTTT 
CAATTTGGAG AGGTTGTAGT TGAATGGAGT CGAGATCAGT ACCATGTTTT GTTTGATTCC 
TACAGAGATA ATGTTGCTGG AAAGTCTTTT CAGACGAGGC TTTGCCTCCC GATGCCCATC 
GATGTGGTGT ACACATGGGT GAATGGCACA GACATAAACC TGCTGAAGGA TCTTCGTGCT 
GTCAGACAGC GACTGGAAGA AGAACAGAAG GCCTTGAGGG AGCGTCTGGG AAAAAATGGC 
TCTGAAATAA CAGAAGCTCC TAAAGGGCGG CCAGAATGTC TACTGTCGCA CTGCATTATG 
GGTCCAGTGC TGGTACTGGA TCCTGCTCTC CCTGCCAACA TCACAGTGAA GGAGCTGCCC 
ACACTTTCAG CCGCCTTCTC TTCTGCTAAA GAGCTCCTGC AGGTGGCCAA ACCTCTGCAT 
CCGTCCAGCA GTGTCACTGC GCTCCTCTTC CACTCTCACA CTGAAGCTGA AAAAGCTCAC 
GCAGACGCTT TGAAGGATCT CCTGACACAC TCCATCAGCA GAGGTTACCT GACGACTGAT 
AAAGAGGCTC CGGGATTGGT CCGCATGCAC ACTCTGGCCT ACTTGAGCGG CTTTCCAGCA 
TCGCTGAAGG AAACTGAGCA GCTCCGGGTC AAACTGCCAG CTGTAGTGAC CAGTAAAACC 
AAAAAGTTGC AGTTATACTC TGAAGCCAGC ATCGCTCTTC TCCACCTGAA CACAGCACAG 
GATTTCACAG ATCTCACACA GCAGGCCAAG AAGAACCTGA CACTGGATGG GAAAGAGCTG 
ACAGTCAGCT CCGCGTTTCT GTTCTGGGAC CTCACTGCCA TCTCACAGTC TAAGCAGGAT 
GAGGATGTTT CTGCCAGTCG CTTTGAAGAC AATGAAGAGC TGCGCTATTC ACTGCGCTCC 
ATCGAAAAGC ACGCGCCCTG GGTGCGGCAC ATCTTTATCG TAACAAACGG ACAGATACCG 
TCTTGGCTTA ACCTGGATAA TCCACGGGTT TCAGTGGTTA CTCACCAGGA TATTTTCCAG 
AATCAGACTC ATCTGCCTAC GTTCAGTTCT CCTGCCATAG AGACCCACAT CCACCGCATC 
CCAGGACTCT CTCAAAAGTT CATCTACCTC AATGATGATG TCATGTTCGG CAAGGATGTG 
TGGCCTGATG ACTTCTATAG CCACTCCAAA GGCCAAAAGG TGTATCTTAC CTGGCCTGTG 
CCAAATTGTG CTGAGGGCTG TCCAGGATCC TGGATCAAAG ACGGCTACTG CGACAAGGCC 
TGCAACAACT CTGCATGTGA CTGGGATGGA GGAGACTGTC AAGGCAGCAG TCGCTTTGGT 
GGAGCAGGTG GCTCTGTGAT CGGCGGTGGG CAGCCCTGGC AGTTTGCAGG TGGTCTTGGA 
GGTCTGGCTG GCATGTCCTT CTGTAATCAG GGCTGTGCTA ACTCTTGGTT GGCTGATAAG 
TTCTGTGACC AGGCATGCAA TGTTCTGGCC TGTGGGTTTG ATGTGGGCGA CTGCGGACAA 
GACAATTTAA ACCAGCTGCA CCGCATCGTG TTGAGGAGGA ACCAGACTCT GTACACCCTT 
CCTCAAGGAG AGCTCAGACC ATATTTCAGT TTCTCCGGCC TGGCGAATCG TGTGTCCGAG 
GCTCATGTAG CCGATAACCA AGTCCTGCGA CACACTTCAG TTGCCAACAA ATGGAAAACC 
ATCCACTTGC TGCTGCTTCC GGGACACAAC GCCACCCAAA TCCACTACAA CATCACCTTC 
CAGAGCACAG ACCACCACGA CTTCATCATG ACCTTCTCAG TCAGTGTTGA CACCCGAGAG 
CTGCCCAAAT CAAACACCTC CACTCCAGTG CGGGACAAAG AAGAAGAGCC CAAACCAACT 
GTAGCGACCC CAGAACCAGA AGTCCCATTC GAAGCTGTTC CCAAAGAAAA ACAAGGCCCT 
AAAGTTAGGG AACAAACACA CGGTGATGTA CAGGTGCCTG TGCTAAATGA GACTCTCTTG 
CCTGACGAAG TGAAGATCGA GTTGAAAAAG CTGAACGAGA AGCTAATGTC AGGTGACATC 
ACTATTAAAG GCTTTAATTT GACCAAAGCA ATGCTGTTGG AGCCCTATAA AGAGAAAGAT 
CAGTTTACAT TAAAACACGA AAAGGACAAA GAAGAAAAGC AGAAACTTTC TCCCGTGGCT 
CAGCTTCAGA ATGAGAGAGC AGCAGCAAGA GTGAAGAGAG AAAAAGGCGA AAATGTTGAT 
CAACTGAAGC CAGTTGCTCC ATCTCTGGTG CCGGTTCAGA TTGATGATGT CACAACCAAA 
GCTCAGTCAC GGTTGTTCAA CATAGAGAAA CCTCACATGT TTCATCTTCT TAGTAACCTG 
AAGGGGAACT TATCTAAGGA GAGGGATTCA GACTCCGAGG GTCATCTCCG CGAGCGGCCA 
ACTGGTCGTA GGCTTCAGTT TTACAGCGAC AGCTTGAACA GAGGTTTCCT GCCGTGGGAG 
AAGAGGAAGT TCTTTCAGGA TCTGATGGAG GAAGTAAATC GTCTCCAGAC TGAACTGCAG 
TACACAGCTG ACCGCACCGC CACTGGACGC AGACTGCAGG ACACTTTTGC CGATTCACTC 
CGATATGTCA ACCGACTTTT AAATGCTCAA TTTGGCTTCA CCTCCCGCAA AGTGCCTGCG 
CACATGCCTC ACATGATAGA CAGACTCATC ATGCAGGAAC TACAGGACAC TTTTCCTCAG 
GAGTTTGATA AGACCTCTTC TCACCGTGTG CGCCACTCTG AGGACATGCA GTTCGCTTTT 
TCTTATTTCT ACTTTTTAAT GAGTGCTGTG CAGCAGCTCA ATATATCTGA AGTGTTTGAT 
GAAATCGACA CGGATCACTC TGGTGTGCTG TCTGACCGAG AGATCCGGAC ACTGGCCACA 
CGCATCCATG AGCTGCCCTT AAGCCTTCAG GATTTGACCA GCCTAGAACA GATGCTTATA 
AACTGCTCAA AGTCTCTTCC ATCCAACCTC ACGCACCTCC ATGCTGTCAG TCCCACCCAG 
GAGGCCTATT ACGACCCCAG CATGCCACCA GTCACCAAAG GTTTAGTGAT CCACTGCAAA 
CCCATCACAG AGAGGATCCA CAAAGCCTTC AAAGACCAGA ATAAATACAA GTTTGAAATT 
ATGGGTGAAG AGGAGATCGC CTTTAAGATG ATACGAACCA ACGTATCTCA TGTAGTCGGG 
CAGCTCGATG ATATTAGGAA GAACCCTAGG AAGTTTATTT GCCTGAATGA CAACATTGAC 
CACATTCATA AGGATGCGGG CACGGTAAAA GCGGTACTGA GGGACTTCTA TGAGTCTATG 
TTCCCACTGC CATCTCAGTT TGAGCTGCCC AGAGAATACA GAAACCGCTT CCTCCACATG 
ACTGAACTGC AGGAGTGGCG AATATACAGA GACAAGCTGA AGTTCTGGAC CCACTGTGTG 
CTGGTGACAT TAGTCGTCTT CACTGTCATG TCTTTCTTTG CTGAGCAGCT GGTCATGCTG 
AAGCGATGGC TCTTCCCAAG ACGCAGAGTC AGCAAAGATG CAAACCCTGA GCGGGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gnptab ( XM_005164744.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164801.1 Danio rerio N-acetylglucosamine-1-phosphate transferase, alpha and beta subunits (gnptab), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa09372
Clone ID Related Accession (Same CDS sequence)	XM_005164744.3 , XM_005164744.4
Accession Version	XM_005164744.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	N-acetylglucosamine-1-phosphotransferase subunits alpha/beta isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005164744.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTCG TTAACTCGCT GTTGAAGCTG CTGCAGCGGC AAACCTACAC CTGCCTGTCC 
CACAGATACG GGCTTTACCT GTGTTTCGGA GGACTGGTGC TCATGATCGT CTCCGCCTTT 
CAATTTGGAG AGGTTGTAGT TGAATGGAGT CGAGATCAGT ACCATGTTTT GTTTGATTCC 
TACAGAGATA ATGTTGCTGG AAAGTCTTTT CAGACGAGGC TTTGCCTCCC GATGCCCATC 
GATGTGGTGT ACACATGGGT GAATGGCACA GACATAAACC TGCTGAAGGA TCTTCGTGCT 
GTCAGACAGC GACTGGAAGA AGAACAGAAG GCCTTGAGGG AGCGTCTGGG AAAAAATGGC 
TCTGAAATAA CAGAAGCTCC TAAAGGGCGG CCAGAATGTC TACTGTCGCA CTGCATTATG 
GGTCCAGTGC TGGTACTGGA TCCTGCTCTC CCTGCCAACA TCACAGTGAA GGAGCTGCCC 
ACACTTTCAG CCGCCTTCTC TTCTGCTAAA GAGCTCCTGC AGGTGGCCAA ACCTCTGCAT 
CCGTCCAGCA GTGTCACTGC GCTCCTCTTC CACTCTCACA CTGAAGCTGA AAAAGCTCAC 
GCAGACGCTT TGAAGGATCT CCTGACACAC TCCATCAGCA GAGGTTACCT GACGACTGAT 
AAAGAGGCTC CGGGATTGGT CCGCATGCAC ACTCTGGCCT ACTTGAGCGG CTTTCCAGCA 
TCGCTGAAGG AAACTGAGCA GCTCCGGGTC AAACTGCCAG CTGTAGTGAC CAGTAAAACC 
AAAAAGTTGC AGTTATACTC TGAAGCCAGC ATCGCTCTTC TCCACCTGAA CACAGCACAG 
GATTTCACAG ATCTCACACA GCAGGCCAAG AAGAACCTGA CACTGGATGG GAAAGAGCTG 
ACAGTCAGCT CCGCGTTTCT GTTCTGGGAC CTCACTGCCA TCTCACAGTC TAAGCAGGAT 
GAGGATGTTT CTGCCAGTCG CTTTGAAGAC AATGAAGAGC TGCGCTATTC ACTGCGCTCC 
ATCGAAAAGC ACGCGCCCTG GGTGCGGCAC ATCTTTATCG TAACAAACGG ACAGATACCG 
TCTTGGCTTA ACCTGGATAA TCCACGGGTT TCAGTGGTTA CTCACCAGGA TATTTTCCAG 
AATCAGACTC ATCTGCCTAC GTTCAGTTCT CCTGCCATAG AGACCCACAT CCACCGCATC 
CCAGGACTCT CTCAAAAGTT CATCTACCTC AATGATGATG TCATGTTCGG CAAGGATGTG 
TGGCCTGATG ACTTCTATAG CCACTCCAAA GGCCAAAAGG TGTATCTTAC CTGGCCTGTG 
CCAAATTGTG CTGAGGGCTG TCCAGGATCC TGGATCAAAG ACGGCTACTG CGACAAGGCC 
TGCAACAACT CTGCATGTGA CTGGGATGGA GGAGACTGTC AAGGCACTGC AGGCAGCAGT 
CGCTTTGGTG GAGCAGGTGG CTCTGTGATC GGCGGTGGGC AGCCCTGGCA GTTTGCAGGT 
GGTCTTGGAG GTCTGGCTGG CATGTCCTTC TGTAATCAGG GCTGTGCTAA CTCTTGGTTG 
GCTGATAAGT TCTGTGACCA GGCATGCAAT GTTCTGGCCT GTGGGTTTGA TGTGGGCGAC 
TGCGGACAAG ACAATTTAAA CCAGCTGCAC CGCATCGTGT TGAGGAGGAA CCAGACTCTG 
TACACCCTTC CTCAAGGAGA GCTCAGACCA TATTTCAGTT TCTCCGGCCT GGCGAATCGT 
GTGTCCGAGG CTCATGTAGC CGATAACCAA GTCCTGCGAC ACACTTCAGT TGCCAACAAA 
TGGAAAACCA TCCACTTGCT GCTGCTTCCG GGACACAACG CCACCCAAAT CCACTACAAC 
ATCACCTTCC AGAGCACAGA CCACCACGAC TTCATCATGA CCTTCTCAGT CAGTGTTGAC 
ACCCGAGAGC TGCCCAAATC AAACACCTCC ACTCCAGTGC GGGACAAAGA AGAAGAGCCC 
AAACCAACTG TAGCGACCCC AGAACCAGAA GTCCCATTCG AAGCTGTTCC CAAAGAAAAA 
CAAGGCCCTA AAGTTAGGGA ACAAACACAC GGTGATGTAC AGGTGCCTGT GCTAAATGAG 
ACTCTCTTGC CTGACGAAGT GAAGATCGAG TTGAAAAAGC TGAACGAGAA GCTAATGTCA 
GGTGACATCA CTATTAAAGG CTTTAATTTG ACCAAAGCAA TGCTGTTGGA GCCCTATAAA 
GAGAAAGATC AGTTTACATT AAAACACGAA AAGGACAAAG AAGAAAAGCA GAAACTTTCT 
CCCGTGGCTC AGCTTCAGAA TGAGAGAGCA GCAGCAAGAG TGAAGAGAGA AAAAGGCGAA 
AATGTTGATC AACTGAAGCC AGTTGCTCCA TCTCTGGTGC CGGTTCAGAT TGATGATGTC 
ACAACCAAAG CTCAGTCACG GTTGTTCAAC ATAGAGAAAC CTCACATGTT TCATCTTCTT 
AGTAACCTGA AGGGGAACTT ATCTAAGGAG AGGGATTCAG ACTCCGAGGG TCATCTCCGC 
GAGCGGCCAA CTGGTCGTAG GCTTCAGTTT TACAGCGACA GCTTGAACAG AGGTTTCCTG 
CCGTGGGAGA AGAGGAAGTT CTTTCAGGAT CTGATGGAGG AAGTAAATCG TCTCCAGACT 
GAACTGCAGT ACACAGCTGA CCGCACCGCC ACTGGACGCA GACTGCAGGA CACTTTTGCC 
GATTCACTCC GATATGTCAA CCGACTTTTA AATGCTCAAT TTGGCTTCAC CTCCCGCAAA 
GTGCCTGCGC ACATGCCTCA CATGATAGAC AGACTCATCA TGCAGGAACT ACAGGACACT 
TTTCCTCAGG AGTTTGATAA GACCTCTTCT CACCGTGTGC GCCACTCTGA GGACATGCAG 
TTCGCTTTTT CTTATTTCTA CTTTTTAATG AGTGCTGTGC AGCAGCTCAA TATATCTGAA 
GTGTTTGATG AAATCGACAC GGATCACTCT GGTGTGCTGT CTGACCGAGA GATCCGGACA 
CTGGCCACAC GCATCCATGA GCTGCCCTTA AGCCTTCAGG ATTTGACCAG CCTAGAACAG 
ATGCTTATAA ACTGCTCAAA GTCTCTTCCA TCCAACCTCA CGCACCTCCA TGCTGTCAGT 
CCCACCCAGG AGGCCTATTA CGACCCCAGC ATGCCACCAG TCACCAAAGG TTTAGTGATC 
CACTGCAAAC CCATCACAGA GAGGATCCAC AAAGCCTTCA AAGACCAGAA TAAATACAAG 
TTTGAAATTA TGGGTGAAGA GGAGATCGCC TTTAAGATGA TACGAACCAA CGTATCTCAT 
GTAGTCGGGC AGCTCGATGA TATTAGGAAG AACCCTAGGA AGTTTATTTG CCTGAATGAC 
AACATTGACC ACATTCATAA GGATGCGGGC ACGGTAAAAG CGGTACTGAG GGACTTCTAT 
GAGTCTATGT TCCCACTGCC ATCTCAGTTT GAGCTGCCCA GAGAATACAG AAACCGCTTC 
CTCCACATGA CTGAACTGCA GGAGTGGCGA ATATACAGAG ACAAGCTGAA GTTCTGGACC 
CACTGTGTGC TGGTGACATT AGTCGTCTTC ACTGTCATGT CTTTCTTTGC TGAGCAGCTG 
GTCATGCTGA AGCGATGGCT CTTCCCAAGA CGCAGAGTCA GCAAAGATGC AAACCCTGAG 
CGGGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164801.1
CDS	217..3885
Translation	MLVVNSLLKLLQRQTYTCLSHRYGLYLCFGGLVLMIVSAFQFGEVVVEWSRDQYHVLFDSYRDNVAGKSFQTRLCLPMPIDVVYTWVNGTDINLLKDLRAVRQRLEEEQKALRERLGKNGSEITEAPKGRPECLLSHCIMGPVLVLDPALPANITVKELPTLSAAFSSAKELLQVAKPLHPSSSVTALLFHSHTEAEKAHADALKDLLTHSISRGYLTTDKEAPGLVRMHTLAYLSGFPASLKETEQLRVKLPAVVTSKTKKLQLYSEASIALLHLNTAQDFTDLTQQAKKNLTLDGKELTVSSAFLFWDLTAISQSKQDEDVSASRFEDNEELRYSLRSIEKHAPWVRHIFIVTNGQIPSWLNLDNPRVSVVTHQDIFQNQTHLPTFSSPAIETHIHRIPGLSQKFIYLNDDVMFGKDVWPDDFYSHSKGQKVYLTWPVPNCAEGCPGSWIKDGYCDKACNNSACDWDGGDCQGTAGSSRFGGAGGSVIGGGQPWQFAGGLGGLAGMSFCNQGCANSWLADKFCDQACNVLACGFDVGDCGQDNLNQLHRIVLRRNQTLYTLPQGELRPYFSFSGLANRVSEAHVADNQVLRHTSVANKWKTIHLLLLPGHNATQIHYNITFQSTDHHDFIMTFSVSVDTRELPKSNTSTPVRDKEEEPKPTVATPEPEVPFEAVPKEKQGPKVREQTHGDVQVPVLNETLLPDEVKIELKKLNEKLMSGDITIKGFNLTKAMLLEPYKEKDQFTLKHEKDKEEKQKLSPVAQLQNERAAARVKREKGENVDQLKPVAPSLVPVQIDDVTTKAQSRLFNIEKPHMFHLLSNLKGNLSKERDSDSEGHLRERPTGRRLQFYSDSLNRGFLPWEKRKFFQDLMEEVNRLQTELQYTADRTATGRRLQDTFADSLRYVNRLLNAQFGFTSRKVPAHMPHMIDRLIMQELQDTFPQEFDKTSSHRVRHSEDMQFAFSYFYFLMSAVQQLNISEVFDEIDTDHSGVLSDREIRTLATRIHELPLSLQDLTSLEQMLINCSKSLPSNLTHLHAVSPTQEAYYDPSMPPVTKGLVIHCKPITERIHKAFKDQNKYKFEIMGEEEIAFKMIRTNVSHVVGQLDDIRKNPRKFICLNDNIDHIHKDAGTVKAVLRDFYESMFPLPSQFELPREYRNRFLHMTELQEWRIYRDKLKFWTHCVLVTLVVFTVMSFFAEQLVMLKRWLFPRRRVSKDANPERV

Target ORF information:

RefSeq Version	XM_005164744.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio N-acetylglucosamine-1-phosphate transferase, alpha and beta subunits (gnptab), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164744.3

ORF Insert Sequence:

ATGTTGGTCG TTAACTCGCT GTTGAAGCTG CTGCAGCGGC AAACCTACAC CTGCCTGTCC 
CACAGATACG GGCTTTACCT GTGTTTCGGA GGACTGGTGC TCATGATCGT CTCCGCCTTT 
CAATTTGGAG AGGTTGTAGT TGAATGGAGT CGAGATCAGT ACCATGTTTT GTTTGATTCC 
TACAGAGATA ATGTTGCTGG AAAGTCTTTT CAGACGAGGC TTTGCCTCCC GATGCCCATC 
GATGTGGTGT ACACATGGGT GAATGGCACA GACATAAACC TGCTGAAGGA TCTTCGTGCT 
GTCAGACAGC GACTGGAAGA AGAACAGAAG GCCTTGAGGG AGCGTCTGGG AAAAAATGGC 
TCTGAAATAA CAGAAGCTCC TAAAGGGCGG CCAGAATGTC TACTGTCGCA CTGCATTATG 
GGTCCAGTGC TGGTACTGGA TCCTGCTCTC CCTGCCAACA TCACAGTGAA GGAGCTGCCC 
ACACTTTCAG CCGCCTTCTC TTCTGCTAAA GAGCTCCTGC AGGTGGCCAA ACCTCTGCAT 
CCGTCCAGCA GTGTCACTGC GCTCCTCTTC CACTCTCACA CTGAAGCTGA AAAAGCTCAC 
GCAGACGCTT TGAAGGATCT CCTGACACAC TCCATCAGCA GAGGTTACCT GACGACTGAT 
AAAGAGGCTC CGGGATTGGT CCGCATGCAC ACTCTGGCCT ACTTGAGCGG CTTTCCAGCA 
TCGCTGAAGG AAACTGAGCA GCTCCGGGTC AAACTGCCAG CTGTAGTGAC CAGTAAAACC 
AAAAAGTTGC AGTTATACTC TGAAGCCAGC ATCGCTCTTC TCCACCTGAA CACAGCACAG 
GATTTCACAG ATCTCACACA GCAGGCCAAG AAGAACCTGA CACTGGATGG GAAAGAGCTG 
ACAGTCAGCT CCGCGTTTCT GTTCTGGGAC CTCACTGCCA TCTCACAGTC TAAGCAGGAT 
GAGGATGTTT CTGCCAGTCG CTTTGAAGAC AATGAAGAGC TGCGCTATTC ACTGCGCTCC 
ATCGAAAAGC ACGCGCCCTG GGTGCGGCAC ATCTTTATCG TAACAAACGG ACAGATACCG 
TCTTGGCTTA ACCTGGATAA TCCACGGGTT TCAGTGGTTA CTCACCAGGA TATTTTCCAG 
AATCAGACTC ATCTGCCTAC GTTCAGTTCT CCTGCCATAG AGACCCACAT CCACCGCATC 
CCAGGACTCT CTCAAAAGTT CATCTACCTC AATGATGATG TCATGTTCGG CAAGGATGTG 
TGGCCTGATG ACTTCTATAG CCACTCCAAA GGCCAAAAGG TGTATCTTAC CTGGCCTGTG 
CCAAATTGTG CTGAGGGCTG TCCAGGATCC TGGATCAAAG ACGGCTACTG CGACAAGGCC 
TGCAACAACT CTGCATGTGA CTGGGATGGA GGAGACTGTC AAGGCACTGC AGGCAGCAGT 
CGCTTTGGTG GAGCAGGTGG CTCTGTGATC GGCGGTGGGC AGCCCTGGCA GTTTGCAGGT 
GGTCTTGGAG GTCTGGCTGG CATGTCCTTC TGTAATCAGG GCTGTGCTAA CTCTTGGTTG 
GCTGATAAGT TCTGTGACCA GGCATGCAAT GTTCTGGCCT GTGGGTTTGA TGTGGGCGAC 
TGCGGACAAG ACAATTTAAA CCAGCTGCAC CGCATCGTGT TGAGGAGGAA CCAGACTCTG 
TACACCCTTC CTCAAGGAGA GCTCAGACCA TATTTCAGTT TCTCCGGCCT GGCGAATCGT 
GTGTCCGAGG CTCATGTAGC CGATAACCAA GTCCTGCGAC ACACTTCAGT TGCCAACAAA 
TGGAAAACCA TCCACTTGCT GCTGCTTCCG GGACACAACG CCACCCAAAT CCACTACAAC 
ATCACCTTCC AGAGCACAGA CCACCACGAC TTCATCATGA CCTTCTCAGT CAGTGTTGAC 
ACCCGAGAGC TGCCCAAATC AAACACCTCC ACTCCAGTGC GGGACAAAGA AGAAGAGCCC 
AAACCAACTG TAGCGACCCC AGAACCAGAA GTCCCATTCG AAGCTGTTCC CAAAGAAAAA 
CAAGGCCCTA AAGTTAGGGA ACAAACACAC GGTGATGTAC AGGTGCCTGT GCTAAATGAG 
ACTCTCTTGC CTGACGAAGT GAAGATCGAG TTGAAAAAGC TGAACGAGAA GCTAATGTCA 
GGTGACATCA CTATTAAAGG CTTTAATTTG ACCAAAGCAA TGCTGTTGGA GCCCTATAAA 
GAGAAAGATC AGTTTACATT AAAACACGAA AAGGACAAAG AAGAAAAGCA GAAACTTTCT 
CCCGTGGCTC AGCTTCAGAA TGAGAGAGCA GCAGCAAGAG TGAAGAGAGA AAAAGGCGAA 
AATGTTGATC AACTGAAGCC AGTTGCTCCA TCTCTGGTGC CGGTTCAGAT TGATGATGTC 
ACAACCAAAG CTCAGTCACG GTTGTTCAAC ATAGAGAAAC CTCACATGTT TCATCTTCTT 
AGTAACCTGA AGGGGAACTT ATCTAAGGAG AGGGATTCAG ACTCCGAGGG TCATCTCCGC 
GAGCGGCCAA CTGGTCGTAG GCTTCAGTTT TACAGCGACA GCTTGAACAG AGGTTTCCTG 
CCGTGGGAGA AGAGGAAGTT CTTTCAGGAT CTGATGGAGG AAGTAAATCG TCTCCAGACT 
GAACTGCAGT ACACAGCTGA CCGCACCGCC ACTGGACGCA GACTGCAGGA CACTTTTGCC 
GATTCACTCC GATATGTCAA CCGACTTTTA AATGCTCAAT TTGGCTTCAC CTCCCGCAAA 
GTGCCTGCGC ACATGCCTCA CATGATAGAC AGACTCATCA TGCAGGAACT ACAGGACACT 
TTTCCTCAGG AGTTTGATAA GACCTCTTCT CACCGTGTGC GCCACTCTGA GGACATGCAG 
TTCGCTTTTT CTTATTTCTA CTTTTTAATG AGTGCTGTGC AGCAGCTCAA TATATCTGAA 
GTGTTTGATG AAATCGACAC GGATCACTCT GGTGTGCTGT CTGACCGAGA GATCCGGACA 
CTGGCCACAC GCATCCATGA GCTGCCCTTA AGCCTTCAGG ATTTGACCAG CCTAGAACAG 
ATGCTTATAA ACTGCTCAAA GTCTCTTCCA TCCAACCTCA CGCACCTCCA TGCTGTCAGT 
CCCACCCAGG AGGCCTATTA CGACCCCAGC ATGCCACCAG TCACCAAAGG TTTAGTGATC 
CACTGCAAAC CCATCACAGA GAGGATCCAC AAAGCCTTCA AAGACCAGAA TAAATACAAG 
TTTGAAATTA TGGGTGAAGA GGAGATCGCC TTTAAGATGA TACGAACCAA CGTATCTCAT 
GTAGTCGGGC AGCTCGATGA TATTAGGAAG AACCCTAGGA AGTTTATTTG CCTGAATGAC 
AACATTGACC ACATTCATAA GGATGCGGGC ACGGTAAAAG CGGTACTGAG GGACTTCTAT 
GAGTCTATGT TCCCACTGCC ATCTCAGTTT GAGCTGCCCA GAGAATACAG AAACCGCTTC 
CTCCACATGA CTGAACTGCA GGAGTGGCGA ATATACAGAG ACAAGCTGAA GTTCTGGACC 
CACTGTGTGC TGGTGACATT AGTCGTCTTC ACTGTCATGT CTTTCTTTGC TGAGCAGCTG 
GTCATGCTGA AGCGATGGCT CTTCCCAAGA CGCAGAGTCA GCAAAGATGC AAACCCTGAG 
CGGGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gnptab ( XM_005164744.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164801.1 Danio rerio N-acetylglucosamine-1-phosphate transferase, alpha and beta subunits (gnptab), transcript variant X1, mRNA.

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CloneID	ODa09372
Clone ID Related Accession (Same CDS sequence)	XM_005164744.3 , XM_005164744.4
Accession Version	XM_005164744.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	N-acetylglucosamine-1-phosphotransferase subunits alpha/beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005164744.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTCG TTAACTCGCT GTTGAAGCTG CTGCAGCGGC AAACCTACAC CTGCCTGTCC 
CACAGATACG GGCTTTACCT GTGTTTCGGA GGACTGGTGC TCATGATCGT CTCCGCCTTT 
CAATTTGGAG AGGTTGTAGT TGAATGGAGT CGAGATCAGT ACCATGTTTT GTTTGATTCC 
TACAGAGATA ATGTTGCTGG AAAGTCTTTT CAGACGAGGC TTTGCCTCCC GATGCCCATC 
GATGTGGTGT ACACATGGGT GAATGGCACA GACATAAACC TGCTGAAGGA TCTTCGTGCT 
GTCAGACAGC GACTGGAAGA AGAACAGAAG GCCTTGAGGG AGCGTCTGGG AAAAAATGGC 
TCTGAAATAA CAGAAGCTCC TAAAGGGCGG CCAGAATGTC TACTGTCGCA CTGCATTATG 
GGTCCAGTGC TGGTACTGGA TCCTGCTCTC CCTGCCAACA TCACAGTGAA GGAGCTGCCC 
ACACTTTCAG CCGCCTTCTC TTCTGCTAAA GAGCTCCTGC AGGTGGCCAA ACCTCTGCAT 
CCGTCCAGCA GTGTCACTGC GCTCCTCTTC CACTCTCACA CTGAAGCTGA AAAAGCTCAC 
GCAGACGCTT TGAAGGATCT CCTGACACAC TCCATCAGCA GAGGTTACCT GACGACTGAT 
AAAGAGGCTC CGGGATTGGT CCGCATGCAC ACTCTGGCCT ACTTGAGCGG CTTTCCAGCA 
TCGCTGAAGG AAACTGAGCA GCTCCGGGTC AAACTGCCAG CTGTAGTGAC CAGTAAAACC 
AAAAAGTTGC AGTTATACTC TGAAGCCAGC ATCGCTCTTC TCCACCTGAA CACAGCACAG 
GATTTCACAG ATCTCACACA GCAGGCCAAG AAGAACCTGA CACTGGATGG GAAAGAGCTG 
ACAGTCAGCT CCGCGTTTCT GTTCTGGGAC CTCACTGCCA TCTCACAGTC TAAGCAGGAT 
GAGGATGTTT CTGCCAGTCG CTTTGAAGAC AATGAAGAGC TGCGCTATTC ACTGCGCTCC 
ATCGAAAAGC ACGCGCCCTG GGTGCGGCAC ATCTTTATCG TAACAAACGG ACAGATACCG 
TCTTGGCTTA ACCTGGATAA TCCACGGGTT TCAGTGGTTA CTCACCAGGA TATTTTCCAG 
AATCAGACTC ATCTGCCTAC GTTCAGTTCT CCTGCCATAG AGACCCACAT CCACCGCATC 
CCAGGACTCT CTCAAAAGTT CATCTACCTC AATGATGATG TCATGTTCGG CAAGGATGTG 
TGGCCTGATG ACTTCTATAG CCACTCCAAA GGCCAAAAGG TGTATCTTAC CTGGCCTGTG 
CCAAATTGTG CTGAGGGCTG TCCAGGATCC TGGATCAAAG ACGGCTACTG CGACAAGGCC 
TGCAACAACT CTGCATGTGA CTGGGATGGA GGAGACTGTC AAGGCACTGC AGGCAGCAGT 
CGCTTTGGTG GAGCAGGTGG CTCTGTGATC GGCGGTGGGC AGCCCTGGCA GTTTGCAGGT 
GGTCTTGGAG GTCTGGCTGG CATGTCCTTC TGTAATCAGG GCTGTGCTAA CTCTTGGTTG 
GCTGATAAGT TCTGTGACCA GGCATGCAAT GTTCTGGCCT GTGGGTTTGA TGTGGGCGAC 
TGCGGACAAG ACAATTTAAA CCAGCTGCAC CGCATCGTGT TGAGGAGGAA CCAGACTCTG 
TACACCCTTC CTCAAGGAGA GCTCAGACCA TATTTCAGTT TCTCCGGCCT GGCGAATCGT 
GTGTCCGAGG CTCATGTAGC CGATAACCAA GTCCTGCGAC ACACTTCAGT TGCCAACAAA 
TGGAAAACCA TCCACTTGCT GCTGCTTCCG GGACACAACG CCACCCAAAT CCACTACAAC 
ATCACCTTCC AGAGCACAGA CCACCACGAC TTCATCATGA CCTTCTCAGT CAGTGTTGAC 
ACCCGAGAGC TGCCCAAATC AAACACCTCC ACTCCAGTGC GGGACAAAGA AGAAGAGCCC 
AAACCAACTG TAGCGACCCC AGAACCAGAA GTCCCATTCG AAGCTGTTCC CAAAGAAAAA 
CAAGGCCCTA AAGTTAGGGA ACAAACACAC GGTGATGTAC AGGTGCCTGT GCTAAATGAG 
ACTCTCTTGC CTGACGAAGT GAAGATCGAG TTGAAAAAGC TGAACGAGAA GCTAATGTCA 
GGTGACATCA CTATTAAAGG CTTTAATTTG ACCAAAGCAA TGCTGTTGGA GCCCTATAAA 
GAGAAAGATC AGTTTACATT AAAACACGAA AAGGACAAAG AAGAAAAGCA GAAACTTTCT 
CCCGTGGCTC AGCTTCAGAA TGAGAGAGCA GCAGCAAGAG TGAAGAGAGA AAAAGGCGAA 
AATGTTGATC AACTGAAGCC AGTTGCTCCA TCTCTGGTGC CGGTTCAGAT TGATGATGTC 
ACAACCAAAG CTCAGTCACG GTTGTTCAAC ATAGAGAAAC CTCACATGTT TCATCTTCTT 
AGTAACCTGA AGGGGAACTT ATCTAAGGAG AGGGATTCAG ACTCCGAGGG TCATCTCCGC 
GAGCGGCCAA CTGGTCGTAG GCTTCAGTTT TACAGCGACA GCTTGAACAG AGGTTTCCTG 
CCGTGGGAGA AGAGGAAGTT CTTTCAGGAT CTGATGGAGG AAGTAAATCG TCTCCAGACT 
GAACTGCAGT ACACAGCTGA CCGCACCGCC ACTGGACGCA GACTGCAGGA CACTTTTGCC 
GATTCACTCC GATATGTCAA CCGACTTTTA AATGCTCAAT TTGGCTTCAC CTCCCGCAAA 
GTGCCTGCGC ACATGCCTCA CATGATAGAC AGACTCATCA TGCAGGAACT ACAGGACACT 
TTTCCTCAGG AGTTTGATAA GACCTCTTCT CACCGTGTGC GCCACTCTGA GGACATGCAG 
TTCGCTTTTT CTTATTTCTA CTTTTTAATG AGTGCTGTGC AGCAGCTCAA TATATCTGAA 
GTGTTTGATG AAATCGACAC GGATCACTCT GGTGTGCTGT CTGACCGAGA GATCCGGACA 
CTGGCCACAC GCATCCATGA GCTGCCCTTA AGCCTTCAGG ATTTGACCAG CCTAGAACAG 
ATGCTTATAA ACTGCTCAAA GTCTCTTCCA TCCAACCTCA CGCACCTCCA TGCTGTCAGT 
CCCACCCAGG AGGCCTATTA CGACCCCAGC ATGCCACCAG TCACCAAAGG TTTAGTGATC 
CACTGCAAAC CCATCACAGA GAGGATCCAC AAAGCCTTCA AAGACCAGAA TAAATACAAG 
TTTGAAATTA TGGGTGAAGA GGAGATCGCC TTTAAGATGA TACGAACCAA CGTATCTCAT 
GTAGTCGGGC AGCTCGATGA TATTAGGAAG AACCCTAGGA AGTTTATTTG CCTGAATGAC 
AACATTGACC ACATTCATAA GGATGCGGGC ACGGTAAAAG CGGTACTGAG GGACTTCTAT 
GAGTCTATGT TCCCACTGCC ATCTCAGTTT GAGCTGCCCA GAGAATACAG AAACCGCTTC 
CTCCACATGA CTGAACTGCA GGAGTGGCGA ATATACAGAG ACAAGCTGAA GTTCTGGACC 
CACTGTGTGC TGGTGACATT AGTCGTCTTC ACTGTCATGT CTTTCTTTGC TGAGCAGCTG 
GTCATGCTGA AGCGATGGCT CTTCCCAAGA CGCAGAGTCA GCAAAGATGC AAACCCTGAG 
CGGGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164801.1
CDS	215..3883
Translation	MLVVNSLLKLLQRQTYTCLSHRYGLYLCFGGLVLMIVSAFQFGEVVVEWSRDQYHVLFDSYRDNVAGKSFQTRLCLPMPIDVVYTWVNGTDINLLKDLRAVRQRLEEEQKALRERLGKNGSEITEAPKGRPECLLSHCIMGPVLVLDPALPANITVKELPTLSAAFSSAKELLQVAKPLHPSSSVTALLFHSHTEAEKAHADALKDLLTHSISRGYLTTDKEAPGLVRMHTLAYLSGFPASLKETEQLRVKLPAVVTSKTKKLQLYSEASIALLHLNTAQDFTDLTQQAKKNLTLDGKELTVSSAFLFWDLTAISQSKQDEDVSASRFEDNEELRYSLRSIEKHAPWVRHIFIVTNGQIPSWLNLDNPRVSVVTHQDIFQNQTHLPTFSSPAIETHIHRIPGLSQKFIYLNDDVMFGKDVWPDDFYSHSKGQKVYLTWPVPNCAEGCPGSWIKDGYCDKACNNSACDWDGGDCQGTAGSSRFGGAGGSVIGGGQPWQFAGGLGGLAGMSFCNQGCANSWLADKFCDQACNVLACGFDVGDCGQDNLNQLHRIVLRRNQTLYTLPQGELRPYFSFSGLANRVSEAHVADNQVLRHTSVANKWKTIHLLLLPGHNATQIHYNITFQSTDHHDFIMTFSVSVDTRELPKSNTSTPVRDKEEEPKPTVATPEPEVPFEAVPKEKQGPKVREQTHGDVQVPVLNETLLPDEVKIELKKLNEKLMSGDITIKGFNLTKAMLLEPYKEKDQFTLKHEKDKEEKQKLSPVAQLQNERAAARVKREKGENVDQLKPVAPSLVPVQIDDVTTKAQSRLFNIEKPHMFHLLSNLKGNLSKERDSDSEGHLRERPTGRRLQFYSDSLNRGFLPWEKRKFFQDLMEEVNRLQTELQYTADRTATGRRLQDTFADSLRYVNRLLNAQFGFTSRKVPAHMPHMIDRLIMQELQDTFPQEFDKTSSHRVRHSEDMQFAFSYFYFLMSAVQQLNISEVFDEIDTDHSGVLSDREIRTLATRIHELPLSLQDLTSLEQMLINCSKSLPSNLTHLHAVSPTQEAYYDPSMPPVTKGLVIHCKPITERIHKAFKDQNKYKFEIMGEEEIAFKMIRTNVSHVVGQLDDIRKNPRKFICLNDNIDHIHKDAGTVKAVLRDFYESMFPLPSQFELPREYRNRFLHMTELQEWRIYRDKLKFWTHCVLVTLVVFTVMSFFAEQLVMLKRWLFPRRRVSKDANPERV

Target ORF information:

RefSeq Version	XM_005164744.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio N-acetylglucosamine-1-phosphate transferase, alpha and beta subunits (gnptab), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164744.4

ORF Insert Sequence:

ATGTTGGTCG TTAACTCGCT GTTGAAGCTG CTGCAGCGGC AAACCTACAC CTGCCTGTCC 
CACAGATACG GGCTTTACCT GTGTTTCGGA GGACTGGTGC TCATGATCGT CTCCGCCTTT 
CAATTTGGAG AGGTTGTAGT TGAATGGAGT CGAGATCAGT ACCATGTTTT GTTTGATTCC 
TACAGAGATA ATGTTGCTGG AAAGTCTTTT CAGACGAGGC TTTGCCTCCC GATGCCCATC 
GATGTGGTGT ACACATGGGT GAATGGCACA GACATAAACC TGCTGAAGGA TCTTCGTGCT 
GTCAGACAGC GACTGGAAGA AGAACAGAAG GCCTTGAGGG AGCGTCTGGG AAAAAATGGC 
TCTGAAATAA CAGAAGCTCC TAAAGGGCGG CCAGAATGTC TACTGTCGCA CTGCATTATG 
GGTCCAGTGC TGGTACTGGA TCCTGCTCTC CCTGCCAACA TCACAGTGAA GGAGCTGCCC 
ACACTTTCAG CCGCCTTCTC TTCTGCTAAA GAGCTCCTGC AGGTGGCCAA ACCTCTGCAT 
CCGTCCAGCA GTGTCACTGC GCTCCTCTTC CACTCTCACA CTGAAGCTGA AAAAGCTCAC 
GCAGACGCTT TGAAGGATCT CCTGACACAC TCCATCAGCA GAGGTTACCT GACGACTGAT 
AAAGAGGCTC CGGGATTGGT CCGCATGCAC ACTCTGGCCT ACTTGAGCGG CTTTCCAGCA 
TCGCTGAAGG AAACTGAGCA GCTCCGGGTC AAACTGCCAG CTGTAGTGAC CAGTAAAACC 
AAAAAGTTGC AGTTATACTC TGAAGCCAGC ATCGCTCTTC TCCACCTGAA CACAGCACAG 
GATTTCACAG ATCTCACACA GCAGGCCAAG AAGAACCTGA CACTGGATGG GAAAGAGCTG 
ACAGTCAGCT CCGCGTTTCT GTTCTGGGAC CTCACTGCCA TCTCACAGTC TAAGCAGGAT 
GAGGATGTTT CTGCCAGTCG CTTTGAAGAC AATGAAGAGC TGCGCTATTC ACTGCGCTCC 
ATCGAAAAGC ACGCGCCCTG GGTGCGGCAC ATCTTTATCG TAACAAACGG ACAGATACCG 
TCTTGGCTTA ACCTGGATAA TCCACGGGTT TCAGTGGTTA CTCACCAGGA TATTTTCCAG 
AATCAGACTC ATCTGCCTAC GTTCAGTTCT CCTGCCATAG AGACCCACAT CCACCGCATC 
CCAGGACTCT CTCAAAAGTT CATCTACCTC AATGATGATG TCATGTTCGG CAAGGATGTG 
TGGCCTGATG ACTTCTATAG CCACTCCAAA GGCCAAAAGG TGTATCTTAC CTGGCCTGTG 
CCAAATTGTG CTGAGGGCTG TCCAGGATCC TGGATCAAAG ACGGCTACTG CGACAAGGCC 
TGCAACAACT CTGCATGTGA CTGGGATGGA GGAGACTGTC AAGGCACTGC AGGCAGCAGT 
CGCTTTGGTG GAGCAGGTGG CTCTGTGATC GGCGGTGGGC AGCCCTGGCA GTTTGCAGGT 
GGTCTTGGAG GTCTGGCTGG CATGTCCTTC TGTAATCAGG GCTGTGCTAA CTCTTGGTTG 
GCTGATAAGT TCTGTGACCA GGCATGCAAT GTTCTGGCCT GTGGGTTTGA TGTGGGCGAC 
TGCGGACAAG ACAATTTAAA CCAGCTGCAC CGCATCGTGT TGAGGAGGAA CCAGACTCTG 
TACACCCTTC CTCAAGGAGA GCTCAGACCA TATTTCAGTT TCTCCGGCCT GGCGAATCGT 
GTGTCCGAGG CTCATGTAGC CGATAACCAA GTCCTGCGAC ACACTTCAGT TGCCAACAAA 
TGGAAAACCA TCCACTTGCT GCTGCTTCCG GGACACAACG CCACCCAAAT CCACTACAAC 
ATCACCTTCC AGAGCACAGA CCACCACGAC TTCATCATGA CCTTCTCAGT CAGTGTTGAC 
ACCCGAGAGC TGCCCAAATC AAACACCTCC ACTCCAGTGC GGGACAAAGA AGAAGAGCCC 
AAACCAACTG TAGCGACCCC AGAACCAGAA GTCCCATTCG AAGCTGTTCC CAAAGAAAAA 
CAAGGCCCTA AAGTTAGGGA ACAAACACAC GGTGATGTAC AGGTGCCTGT GCTAAATGAG 
ACTCTCTTGC CTGACGAAGT GAAGATCGAG TTGAAAAAGC TGAACGAGAA GCTAATGTCA 
GGTGACATCA CTATTAAAGG CTTTAATTTG ACCAAAGCAA TGCTGTTGGA GCCCTATAAA 
GAGAAAGATC AGTTTACATT AAAACACGAA AAGGACAAAG AAGAAAAGCA GAAACTTTCT 
CCCGTGGCTC AGCTTCAGAA TGAGAGAGCA GCAGCAAGAG TGAAGAGAGA AAAAGGCGAA 
AATGTTGATC AACTGAAGCC AGTTGCTCCA TCTCTGGTGC CGGTTCAGAT TGATGATGTC 
ACAACCAAAG CTCAGTCACG GTTGTTCAAC ATAGAGAAAC CTCACATGTT TCATCTTCTT 
AGTAACCTGA AGGGGAACTT ATCTAAGGAG AGGGATTCAG ACTCCGAGGG TCATCTCCGC 
GAGCGGCCAA CTGGTCGTAG GCTTCAGTTT TACAGCGACA GCTTGAACAG AGGTTTCCTG 
CCGTGGGAGA AGAGGAAGTT CTTTCAGGAT CTGATGGAGG AAGTAAATCG TCTCCAGACT 
GAACTGCAGT ACACAGCTGA CCGCACCGCC ACTGGACGCA GACTGCAGGA CACTTTTGCC 
GATTCACTCC GATATGTCAA CCGACTTTTA AATGCTCAAT TTGGCTTCAC CTCCCGCAAA 
GTGCCTGCGC ACATGCCTCA CATGATAGAC AGACTCATCA TGCAGGAACT ACAGGACACT 
TTTCCTCAGG AGTTTGATAA GACCTCTTCT CACCGTGTGC GCCACTCTGA GGACATGCAG 
TTCGCTTTTT CTTATTTCTA CTTTTTAATG AGTGCTGTGC AGCAGCTCAA TATATCTGAA 
GTGTTTGATG AAATCGACAC GGATCACTCT GGTGTGCTGT CTGACCGAGA GATCCGGACA 
CTGGCCACAC GCATCCATGA GCTGCCCTTA AGCCTTCAGG ATTTGACCAG CCTAGAACAG 
ATGCTTATAA ACTGCTCAAA GTCTCTTCCA TCCAACCTCA CGCACCTCCA TGCTGTCAGT 
CCCACCCAGG AGGCCTATTA CGACCCCAGC ATGCCACCAG TCACCAAAGG TTTAGTGATC 
CACTGCAAAC CCATCACAGA GAGGATCCAC AAAGCCTTCA AAGACCAGAA TAAATACAAG 
TTTGAAATTA TGGGTGAAGA GGAGATCGCC TTTAAGATGA TACGAACCAA CGTATCTCAT 
GTAGTCGGGC AGCTCGATGA TATTAGGAAG AACCCTAGGA AGTTTATTTG CCTGAATGAC 
AACATTGACC ACATTCATAA GGATGCGGGC ACGGTAAAAG CGGTACTGAG GGACTTCTAT 
GAGTCTATGT TCCCACTGCC ATCTCAGTTT GAGCTGCCCA GAGAATACAG AAACCGCTTC 
CTCCACATGA CTGAACTGCA GGAGTGGCGA ATATACAGAG ACAAGCTGAA GTTCTGGACC 
CACTGTGTGC TGGTGACATT AGTCGTCTTC ACTGTCATGT CTTTCTTTGC TGAGCAGCTG 
GTCATGCTGA AGCGATGGCT CTTCCCAAGA CGCAGAGTCA GCAAAGATGC AAACCCTGAG 
CGGGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Enzyme-specific differences in mannose phosphorylation between GlcNAc-1-phosphotransferase αβ and γ subunit deficient zebrafish support cathepsin proteases as early mediators of mucolipidosis pathology.
Biochimica et biophysica acta1860(9)1845-53(2016 09)
Flanagan-Steet H,Matheny C,Petrey A,Parker J,Steet R

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Cathepsin-Mediated Alterations in TGFß-Related Signaling Underlie Disrupted Cartilage and Bone Maturation Associated With Impaired Lysosomal Targeting.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research31(3)535-48(2016 Mar)
Flanagan-Steet H,Aarnio M,Kwan B,Guihard P,Petrey A,Haskins M,Blanchard F,Steet R

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Analysis of mucolipidosis II/III GNPTAB missense mutations identifies domains of UDP-GlcNAc:lysosomal enzyme GlcNAc-1-phosphotransferase involved in catalytic function and lysosomal enzyme recognition.
The Journal of biological chemistry290(5)3045-56(2015 Jan)
Qian Y,van Meel E,Flanagan-Steet H,Yox A,Steet R,Kornfeld S

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gnptab cDNA ORF clone, Danio rerio(zebrafish)

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