smg5 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	smg5
Entrez Gene ID	553261
Full Name	SMG5 nonsense mediated mRNA decay factor
Synonyms	zgc:113841
General protein information	Preferred Names SMG5 nonsense mediated mRNA decay factor Names protein SMG5 Est1p-like protein B SMG5 homolog nonsense mediated mRNA decay factor Smg-5 homolog, nonsense mediated mRNA decay factor
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001024923.1	NP_001020094.1	protein SMG5

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Processing of fish Ig heavy chain transcripts: diverse splicing patterns and unusual nonsense mediated decay.
Quiniou SM, Wilson M, Boudinot P
Developmental and comparative immunology35(9)949-58(2011 Sep)

Nonsense-mediated mRNA decay effectors are essential for zebrafish embryonic development and survival.
Wittkopp N, Huntzinger E, Weiler C, Saulière J, Schmidt S, Sonawane M, Izaurralde E
Molecular and cellular biology29(13)3517-28(2009 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following smg5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the smg5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa02693	NM_001024923.1 Latest version!	Danio rerio SMG5 nonsense mediated mRNA decay factor (smg5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

smg5 ( NM_001024923.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001020094.1 Danio rerio SMG5 nonsense mediated mRNA decay factor (smg5), mRNA.

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CloneID	ODa02693
Clone ID Related Accession (Same CDS sequence)	NM_001024923.1
Accession Version	NM_001024923.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1542902400000
Organism	Danio rerio(zebrafish)
Product	protein SMG5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC096955.1. On Jul 6, 2005 this sequence version replaced XM_678076.1. ##Evidence-Data-START## Transcript exon combination :: BC096955.1, FM993108.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGAGCGGAC CGAGTCAGGA CAGCGAGCCG GAGGCCAAAG TGCTTCATAT TAAACGGCTG 
TACAGGGCTG TGGTGGAAAC GGTGCATAAG TTGGACATTA TAATCAGCGG CAAGTCGTCG 
TACAGGGAGG TCTTCAAACC TGAGAACATC AGCTTGAGGA ACAAATTGCG AGAACTTTGC 
GTAAAGCTGA TGTTCCTGCA TCCTGTGGAT TATGGACGCA AGGCAGAAGA GCTGCTGTGG 
AGGAAAGTCT ACTATGAGGT CATTCAGGTG ATCAAGACCA ACAAGAAGCA TATTCACAGC 
CGCAGTGCTC TGGAGTGTGC CTACAGGACG CATCTGATTG CCGGGGTGGG CTTTTTTCAA 
CACCTGCTGC TCTACATCCA GTCACACTAT CAGCTGGAGT TACAGGACTG CATAGACTGG 
ACACATGTCA CCGACCCGCT CATCGGTCGT AAGAAACCAG TCTCAGCCAC CTCTAAAGAG 
ATGGAATGGG CCCAGATGGC GTGTCACAGA TGCCTTGTGT ACTTGGGAGA TCTCGCGCGC 
TACCAGAATG AACTGGCGGG AGTGGAGGCC GAGCAGCTGG CGGAGCGTTT CTATCACCAG 
GCCCTGTCTG TCGCACCTCA CGTCGGCATG CCGTTTAATC AGTTGGGTAC GCTTGCCGGA 
AGTAAATTTT ATAATGTGGA GGCTACTTAC TACTACTTGC GCTGTATTCA GTCAGAGACT 
CCCTTCGACG GTGCGTATGG GAATTTAAAG CGTCTGTTTG ACAAGGCATC CAAGATGTAC 
CACCAGGTGA AGAAACAAGA AATGAAGAAG CTTTCACCAT CTCGACAGAG GTCAAAAGAC 
ATCAAAAGAC TGCTGGTCAG CTTCATGTAT TTGCAGAGTT TGTTACAGCC CAAGAACAGT 
CTGATGGAGA CGGAGCTGAC GTCGCTGTGC CAGTCTGTGC TGGAAGACTT TAACCTGGTG 
CTTTTCTACC TGCCTCTTCC TGCTCATGGC AGCCAGTCGG CCAGCGAGGA GGAGGAAGAG 
CACGACTCTG TTGGTTCTGT GCTTCCAGAC AGCCTTATTT TCAAGATGGT GGTGATTTGT 
CTGATGGTGG TGCACAGCCT GAAGCGAGGG GCTTCAAAGC AGTACAGCGC CTCCATCGCT 
TTCACATTGG CTCTGTTCTC CCATCTGGTC AATCATGTCA ACATTCGACT GCAAGCTGAA 
TTGGAGGAGG GAGAGAGTGA TGTCCCAGCC CTGCGCACTG ATAACACTGA TGACGCTGAT 
GCCAGAGATC ACAGTAGCGC TGCTCTCTCA GAGGAGCGAA CCCTGCAGAA CGGCTCTCTG 
GAGGAGGATG ACGATGAAGA GGAGGAGGAA AAGGAGAACG GGGAAGACGA GCCTAAAGGC 
AACGGAAGAA CTGTGGCGAC AAAGAAAAAC CAGGAGAAGA AGAGATCAGC CGAGGAGAAG 
CAAAAACAGA AGCGGAAGTT CTCAAGGCTC TCCATGCTTC GCCGAAGACG CTGTGCTCAC 
AAGGAGGATG AAAGCGACCT GAGTGAGGGC TTTGAGAGCG ATGAAGAGGA GGAGGAGGAG 
GAGGAAGGTG GTGGCGGTGG TCTGGTTGAT GGTCTGGGAG CCCCCAGGGT CCAGATGAAC 
TCTTTAGGAA GAGACACACG GAAGGGGCCA CTGCCGGAGG ACGGCGGCTG GGAAAGCGGT 
TCAGAGGAGG ATGAGGGTGG CACGGCTTTT GACGTGGAGA CAGACTCTGA CATGAACAGT 
CAGGAATCGC GATCGGATCT GGAAGACATG GAGGATGCTG AAAATGCACC ACAACAGCCA 
CAACAAGAGG AAAATGAGCA GCCGCAAACG TCTAGAGAGG AAAACGCCAC CCCTCCAGTA 
ACCAATGGGC CGTCTGTGTC TAATGATGCC AGTATCAGCA GTAACCTGCA GGCCATGTCC 
TCGCAGCTTT TTCAGGCCAA ACGCTGCTTC CGGCTCGCCC CTACATTCAG CAATGTGCTG 
CTCCGCCCTG CAACTACCAC TCCCCCTGCC CCAGAATTAA ACCCCGCCCC AGTCCAAGAA 
ATCACACCCC CTACTGGGGA AACGCCAAGA AACACGAGTC CAGAGATTGC AAACGGAACC 
AATGATAAGG ATCCTGACTC TGATTCTGAG GGCAGCGAAC ACAGCAACCA TTCGTTTCAC 
AGTGAGAAAA CTCTTTCTGA GCGACTGGAG ATTCTGACCA ATCAGGGTCT TATTCAGGTG 
GTGAAGGTGT TTTTGGATTG GCTGAGGACG AACACAGACA TCATCCTAAT GTGTGCTCAG 
AGTTCTCAAA GCTTGTGGAA CAGGCTGTCA GTGCTGCTTA ATTTACTTCC CGAGGGAAGC 
AAGATCTTAG AAACAGATAT AGGTCTGAAT AAGGATGTAA CGGAGCTTTT AAGTGAGTGT 
GAGCACCCTA GTTTGGCCCA GACGTTGTTG TTACCTGAAG ATGTGGCTCT CCGTCATCTT 
CCTGCCCTCA GCGTAGCCCA TCGATGCCTG GACTTCACTA GCCAGAGACC CCCTCTTACC 
CCCCTGGATG AGTGTGTGGT GCGCGTGTGC TGTATTCGCA GCTTCGGTCA CTTCCTCACT 
AATCTCCAAG GCAACGTGCT ACACTTCAAC CCGGAGGCGG GCATCTTCAC AAGCATCAGC 
CAATCAGAAC AGGACAACCT GGTTCAGCAG GCCAAGGCCC AATTCCGCAT GGCGGAGGAG 
GAAGCTCGCA GAAACCGGCT CATGAGAGAC ATGGCCCAGC TGCGCCTGCA GTTGGAGGTT 
TCTCAGCTGG AGGGCAGTCT GCAGCAGCCT AAAGCCCAGT CCTCCATGTC TCCTTATCTG 
GTGCCAGATA CTGCTGTCCT CTGCCAGCAC CTGGGGCTGC TCAGACAGCT GGCTGCCAGC 
GGATGCTTCA TCATCATCAT CCCTCGCACA GTGATTGACG GTCTGGACAT GCTGAAGAAA 
GAGAACTCTG GCGCAAGAGA TGGCATCCGG TTCCTTGAGA CAGAGTTTCG AAAAGGGAAT 
AGGTACATCC GCTGTCAGAA AGAGTCAGGA AGAAGCTTTG AGAGGGACAA ACTAAAACGT 
CAGGACACAG AAGCCTGGCA CCTATATAAG ACGGTGGACA GCTGTCGTCA GCTGACGGGA 
TCTCAGAGCA GTGGAGATGA AGACACCGCC GGCATGGTCA CCATTCTCAC AGGACACTCA 
TTGGAGGAGC TGAGTGAAAG ATCGGCTTCC ATGAAGGCGG CTGTCCAGGC GGTAGCAGCA 
GCAGGCATGG AGCTAAAAAA CATCATCGAA TTTTACCGGC AATGGAAAGA GATGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020094.1
CDS	315..3614
Translation	MSGPSQDSEPEAKVLHIKRLYRAVVETVHKLDIIISGKSSYREVFKPENISLRNKLRELCVKLMFLHPVDYGRKAEELLWRKVYYEVIQVIKTNKKHIHSRSALECAYRTHLIAGVGFFQHLLLYIQSHYQLELQDCIDWTHVTDPLIGRKKPVSATSKEMEWAQMACHRCLVYLGDLARYQNELAGVEAEQLAERFYHQALSVAPHVGMPFNQLGTLAGSKFYNVEATYYYLRCIQSETPFDGAYGNLKRLFDKASKMYHQVKKQEMKKLSPSRQRSKDIKRLLVSFMYLQSLLQPKNSLMETELTSLCQSVLEDFNLVLFYLPLPAHGSQSASEEEEEHDSVGSVLPDSLIFKMVVICLMVVHSLKRGASKQYSASIAFTLALFSHLVNHVNIRLQAELEEGESDVPALRTDNTDDADARDHSSAALSEERTLQNGSLEEDDDEEEEEKENGEDEPKGNGRTVATKKNQEKKRSAEEKQKQKRKFSRLSMLRRRRCAHKEDESDLSEGFESDEEEEEEEEGGGGGLVDGLGAPRVQMNSLGRDTRKGPLPEDGGWESGSEEDEGGTAFDVETDSDMNSQESRSDLEDMEDAENAPQQPQQEENEQPQTSREENATPPVTNGPSVSNDASISSNLQAMSSQLFQAKRCFRLAPTFSNVLLRPATTTPPAPELNPAPVQEITPPTGETPRNTSPEIANGTNDKDPDSDSEGSEHSNHSFHSEKTLSERLEILTNQGLIQVVKVFLDWLRTNTDIILMCAQSSQSLWNRLSVLLNLLPEGSKILETDIGLNKDVTELLSECEHPSLAQTLLLPEDVALRHLPALSVAHRCLDFTSQRPPLTPLDECVVRVCCIRSFGHFLTNLQGNVLHFNPEAGIFTSISQSEQDNLVQQAKAQFRMAEEEARRNRLMRDMAQLRLQLEVSQLEGSLQQPKAQSSMSPYLVPDTAVLCQHLGLLRQLAASGCFIIIIPRTVIDGLDMLKKENSGARDGIRFLETEFRKGNRYIRCQKESGRSFERDKLKRQDTEAWHLYKTVDSCRQLTGSQSSGDEDTAGMVTILTGHSLEELSERSASMKAAVQAVAAAGMELKNIIEFYRQWKEMG

Target ORF information:

RefSeq Version	NM_001024923.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SMG5 nonsense mediated mRNA decay factor (smg5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024923.1

ORF Insert Sequence:

ATGAGCGGAC CGAGTCAGGA CAGCGAGCCG GAGGCCAAAG TGCTTCATAT TAAACGGCTG 
TACAGGGCTG TGGTGGAAAC GGTGCATAAG TTGGACATTA TAATCAGCGG CAAGTCGTCG 
TACAGGGAGG TCTTCAAACC TGAGAACATC AGCTTGAGGA ACAAATTGCG AGAACTTTGC 
GTAAAGCTGA TGTTCCTGCA TCCTGTGGAT TATGGACGCA AGGCAGAAGA GCTGCTGTGG 
AGGAAAGTCT ACTATGAGGT CATTCAGGTG ATCAAGACCA ACAAGAAGCA TATTCACAGC 
CGCAGTGCTC TGGAGTGTGC CTACAGGACG CATCTGATTG CCGGGGTGGG CTTTTTTCAA 
CACCTGCTGC TCTACATCCA GTCACACTAT CAGCTGGAGT TACAGGACTG CATAGACTGG 
ACACATGTCA CCGACCCGCT CATCGGTCGT AAGAAACCAG TCTCAGCCAC CTCTAAAGAG 
ATGGAATGGG CCCAGATGGC GTGTCACAGA TGCCTTGTGT ACTTGGGAGA TCTCGCGCGC 
TACCAGAATG AACTGGCGGG AGTGGAGGCC GAGCAGCTGG CGGAGCGTTT CTATCACCAG 
GCCCTGTCTG TCGCACCTCA CGTCGGCATG CCGTTTAATC AGTTGGGTAC GCTTGCCGGA 
AGTAAATTTT ATAATGTGGA GGCTACTTAC TACTACTTGC GCTGTATTCA GTCAGAGACT 
CCCTTCGACG GTGCGTATGG GAATTTAAAG CGTCTGTTTG ACAAGGCATC CAAGATGTAC 
CACCAGGTGA AGAAACAAGA AATGAAGAAG CTTTCACCAT CTCGACAGAG GTCAAAAGAC 
ATCAAAAGAC TGCTGGTCAG CTTCATGTAT TTGCAGAGTT TGTTACAGCC CAAGAACAGT 
CTGATGGAGA CGGAGCTGAC GTCGCTGTGC CAGTCTGTGC TGGAAGACTT TAACCTGGTG 
CTTTTCTACC TGCCTCTTCC TGCTCATGGC AGCCAGTCGG CCAGCGAGGA GGAGGAAGAG 
CACGACTCTG TTGGTTCTGT GCTTCCAGAC AGCCTTATTT TCAAGATGGT GGTGATTTGT 
CTGATGGTGG TGCACAGCCT GAAGCGAGGG GCTTCAAAGC AGTACAGCGC CTCCATCGCT 
TTCACATTGG CTCTGTTCTC CCATCTGGTC AATCATGTCA ACATTCGACT GCAAGCTGAA 
TTGGAGGAGG GAGAGAGTGA TGTCCCAGCC CTGCGCACTG ATAACACTGA TGACGCTGAT 
GCCAGAGATC ACAGTAGCGC TGCTCTCTCA GAGGAGCGAA CCCTGCAGAA CGGCTCTCTG 
GAGGAGGATG ACGATGAAGA GGAGGAGGAA AAGGAGAACG GGGAAGACGA GCCTAAAGGC 
AACGGAAGAA CTGTGGCGAC AAAGAAAAAC CAGGAGAAGA AGAGATCAGC CGAGGAGAAG 
CAAAAACAGA AGCGGAAGTT CTCAAGGCTC TCCATGCTTC GCCGAAGACG CTGTGCTCAC 
AAGGAGGATG AAAGCGACCT GAGTGAGGGC TTTGAGAGCG ATGAAGAGGA GGAGGAGGAG 
GAGGAAGGTG GTGGCGGTGG TCTGGTTGAT GGTCTGGGAG CCCCCAGGGT CCAGATGAAC 
TCTTTAGGAA GAGACACACG GAAGGGGCCA CTGCCGGAGG ACGGCGGCTG GGAAAGCGGT 
TCAGAGGAGG ATGAGGGTGG CACGGCTTTT GACGTGGAGA CAGACTCTGA CATGAACAGT 
CAGGAATCGC GATCGGATCT GGAAGACATG GAGGATGCTG AAAATGCACC ACAACAGCCA 
CAACAAGAGG AAAATGAGCA GCCGCAAACG TCTAGAGAGG AAAACGCCAC CCCTCCAGTA 
ACCAATGGGC CGTCTGTGTC TAATGATGCC AGTATCAGCA GTAACCTGCA GGCCATGTCC 
TCGCAGCTTT TTCAGGCCAA ACGCTGCTTC CGGCTCGCCC CTACATTCAG CAATGTGCTG 
CTCCGCCCTG CAACTACCAC TCCCCCTGCC CCAGAATTAA ACCCCGCCCC AGTCCAAGAA 
ATCACACCCC CTACTGGGGA AACGCCAAGA AACACGAGTC CAGAGATTGC AAACGGAACC 
AATGATAAGG ATCCTGACTC TGATTCTGAG GGCAGCGAAC ACAGCAACCA TTCGTTTCAC 
AGTGAGAAAA CTCTTTCTGA GCGACTGGAG ATTCTGACCA ATCAGGGTCT TATTCAGGTG 
GTGAAGGTGT TTTTGGATTG GCTGAGGACG AACACAGACA TCATCCTAAT GTGTGCTCAG 
AGTTCTCAAA GCTTGTGGAA CAGGCTGTCA GTGCTGCTTA ATTTACTTCC CGAGGGAAGC 
AAGATCTTAG AAACAGATAT AGGTCTGAAT AAGGATGTAA CGGAGCTTTT AAGTGAGTGT 
GAGCACCCTA GTTTGGCCCA GACGTTGTTG TTACCTGAAG ATGTGGCTCT CCGTCATCTT 
CCTGCCCTCA GCGTAGCCCA TCGATGCCTG GACTTCACTA GCCAGAGACC CCCTCTTACC 
CCCCTGGATG AGTGTGTGGT GCGCGTGTGC TGTATTCGCA GCTTCGGTCA CTTCCTCACT 
AATCTCCAAG GCAACGTGCT ACACTTCAAC CCGGAGGCGG GCATCTTCAC AAGCATCAGC 
CAATCAGAAC AGGACAACCT GGTTCAGCAG GCCAAGGCCC AATTCCGCAT GGCGGAGGAG 
GAAGCTCGCA GAAACCGGCT CATGAGAGAC ATGGCCCAGC TGCGCCTGCA GTTGGAGGTT 
TCTCAGCTGG AGGGCAGTCT GCAGCAGCCT AAAGCCCAGT CCTCCATGTC TCCTTATCTG 
GTGCCAGATA CTGCTGTCCT CTGCCAGCAC CTGGGGCTGC TCAGACAGCT GGCTGCCAGC 
GGATGCTTCA TCATCATCAT CCCTCGCACA GTGATTGACG GTCTGGACAT GCTGAAGAAA 
GAGAACTCTG GCGCAAGAGA TGGCATCCGG TTCCTTGAGA CAGAGTTTCG AAAAGGGAAT 
AGGTACATCC GCTGTCAGAA AGAGTCAGGA AGAAGCTTTG AGAGGGACAA ACTAAAACGT 
CAGGACACAG AAGCCTGGCA CCTATATAAG ACGGTGGACA GCTGTCGTCA GCTGACGGGA 
TCTCAGAGCA GTGGAGATGA AGACACCGCC GGCATGGTCA CCATTCTCAC AGGACACTCA 
TTGGAGGAGC TGAGTGAAAG ATCGGCTTCC ATGAAGGCGG CTGTCCAGGC GGTAGCAGCA 
GCAGGCATGG AGCTAAAAAA CATCATCGAA TTTTACCGGC AATGGAAAGA GATGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Processing of fish Ig heavy chain transcripts: diverse splicing patterns and unusual nonsense mediated decay.
Developmental and comparative immunology35(9)949-58(2011 Sep)
Quiniou SM,Wilson M,Boudinot P

Nonsense-mediated mRNA decay effectors are essential for zebrafish embryonic development and survival.
Molecular and cellular biology29(13)3517-28(2009 Jul)
Wittkopp N,Huntzinger E,Weiler C,Saulière J,Schmidt S,Sonawane M,Izaurralde E

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smg5 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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