rad50 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	rad50
Entrez Gene ID	553029
Full Name	RAD50 homolog, double strand break repair protein
Synonyms	im:6906849,si:ch211-79h18.3
General protein information	Preferred Names RAD50 homolog, double strand break repair protein Names DNA repair protein RAD50
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_691767.7	XP_696859.4	DNA repair protein RAD50
XM_005167938.3	XP_005167995.1	DNA repair protein RAD50
XM_005167938.4	XP_005167995.1	DNA repair protein RAD50
XM_691767.8	XP_696859.4	DNA repair protein RAD50

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os02g0497500	NP_001046893.1
Xenopus tropicalis (tropical clawed frog)	rad50	XP_004912891.1
Macaca mulatta (Rhesus monkey)	RAD50	XP_001099384.2
Gallus gallus (chicken)	RAD50	XP_004945072.1
Arabidopsis thaliana (thale cress)	RAD50	NP_565733.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003676	XP_313457.4
Homo sapiens (human)	RAD50	NP_005723.2
Rattus norvegicus (Norway rat)	Rad50	NP_071582.1
Neurospora crassa	NCU00901	XP_963818.1
Pan troglodytes (chimpanzee)	RAD50	XP_001163412.1
Canis lupus familiaris (dog)	RAD50	XP_531901.2
Magnaporthe oryzae (rice blast fungus)	MGG_11387	XP_003713570.1
Bos taurus (cattle)	RAD50	NP_001193797.1
Mus musculus (house mouse)	Rad50	NP_033038.2
Danio rerio (zebrafish)	rad50	XP_005167995.1
Drosophila melanogaster (fruit fly)	rad50	NP_001246461.1

Gene Ontology

The following rad50 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rad50 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa15615	XM_005167938.4 Latest version!	Danio rerio RAD50 homolog, double strand break repair protein (rad50), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa15615	XM_691767.8 Latest version!	Danio rerio RAD50 homolog, double strand break repair protein (rad50), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
ODa15615	XM_005167938.3	Danio rerio RAD50 homolog, double strand break repair protein (rad50), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa15615	XM_691767.7	Danio rerio RAD50 homolog, double strand break repair protein (rad50), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rad50 ( XM_691767.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_696859.4 Danio rerio RAD50 homolog, double strand break repair protein (rad50), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa15615
Clone ID Related Accession (Same CDS sequence)	XM_691767.7 , XM_005167938.3 , XM_005167938.4 , XM_691767.8
Accession Version	XM_691767.7	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	DNA repair protein RAD50
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_691767.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGA TTGAGAAGAT GAGTATTCTG GGAGTGAGGA GTTTTGGAGT GGAGGATAAA 
GATAAACAAG TGATCTCCTT CTTCAGCCCT CTCACGGTGC TTGTGGGACC TAATGGAGCT 
GGCAAAACGA CCATCATTGA GTGTCTCAAG TACATCACAT CAGGAGACTT TCCACCAGGA 
AGTAAAGGAA ACACTTTTGT CCATGATCCC AAAGATGCTC ATGAGACTGA TGTACGGGCA 
CAGATCAGAC TCCAGTTTAG AGATGTGAAT GGAGATGCTG TGGCTGTGCA AAGATCTATG 
CAGTGCACAC AGAAAGGCAA GAAAACAGAA TTCAAAACGC TTGAGGGCGT CATTACGAGA 
ATAAAGCATG GTGAGAAAGT AAGCCTGAGC TCTAAATGTG CTGAGATAGA CCGTGAGATG 
ATCTCTTCCC TGGGCGTCTC CAGAGCTGTT CTCAACCATG TCATCTTTTG CCACCAAGAA 
GAGTCCAACT GGCCTCTCAG TGAAGGGAAA GCCCTCAAGC AGAAGTTTGA TGAGATTTTC 
TCAGCCACAA GGTACATTAA GGTGCTGGAG ACCTTGCGGA CGCTGAGGCA GAAGCAGACC 
AACACTGTTA AATCCTGCCA GATGGAGCTC AAGTACCTGA AACAGAACAA AGATAAAGCC 
CAAGAGATTC GAGAGCTGCT TTCCACCAAA GAAACTCAGC TAGCTTCCTC CAAAGAGAGT 
GTCAATCGCA TCGAGGGTCA GATTGACCCT CTGGAGAGAC GACTAAATGA CATAGAAAGC 
AGCCTCGGCA AAGTGATGAA ATTGGACAAC GACATCAAAG CTCTGGACAG TAGGAAGAAA 
CAGATGGAGG ATGATAACCG GGAGCTAGAG GAAAAGATGG AACAGGTGTT TCAGGGCTCA 
GATGATCAGC TACAGGACAT GTATCAGAAT CACCAGCGCA CCGTGAAAGA GAAGGAAAAA 
AGACTTGTAG AGTGCCAACG TGAGCTTGAA AGGGCTGGTC GAGAATGCCA GAGGATGAAC 
CGCATCAAGT CTGAGCTGCT AGTGGAGCAA GGTCGTCTGC AGTTGGAGGC AGATAGACAC 
ACACAGAACA TTAAAAAGAG AGACACCCAG GTAAAGACTT TGGCGTCTTT CCTGGAGTTG 
GAGGGCTATG ATCGAACGCC ACTTAGTGAG CGACAACTCC AAAGCTTTTA CAGACAAATC 
AAAGAAAGAC TGGACCAGGA CTCGGAAGCC CTCAACCAAA CTATGCATGA CATGCAGCAG 
AAAGAGACCC AGAAACAGCA CAACATTGAT GATCTCCGGG ATAAAAAGAC AGGTCTAGAG 
AGAACCATTG AGCTAAAGAA AGACCTACAG GCCAAAAAGC AACAAGAACT GAAGAATATC 
AAGTCTGACC TTCAAAAACT GGAGGGCTCC TCCAACAGAC TGCAAGAGCT GGACACTGAG 
CTTCAGAAAG CGGAGCGTGA GCTTGATAAT GCTGTGCAGG CCTGTACAGT GGACAGTCTG 
AAGGTCGAGG TAACTGAACT ACTGAAGGAA AAGGCTCAAC TGGATCAGGC ACAGCGCAAA 
CTGGACCAAG AGATGGAAAT GCTCAACACA CACACCACGG CACGTGCACA GATGGACATG 
ATGAAGAAAA CCAAGATGGA CAAAGAGGAG CAGGTGAGGA AGATCAAGTC GAGACATAAT 
GAAGAGCTGG TGTCTCTGCT GGGACACTTT CCAAACAAGA AAGAGCTGGA GGACTGGATC 
TACTCCAAAT CCAGAGAGAT CAAATCCACT AGAGAGCAGA TCACCAAAAT GAATAAAGAG 
CTTGCATCTG GAGAACAGAA GAAGAGTCAT TACACTGCAG AAATCAAACG AAAAGAAGAG 
CAGCTGGCCA AATATGAAGA GAGACTTTTT AATGTTTGTG GAAGCCAGGA TTTCCAGTCA 
GACTTAAGCA AACTGGAGGA TGAGTTGGAA AAGTGCTCCA AACAGAGAGC CATGTTGGCA 
GGTGCCACAG CTGTGTACAG TCAGTTCATC AGTCAGCTGA CGGAGGAAGG TGACCCATGC 
TGCCCCGTAT GTCAGCGCGT GTTCCCATCA GAGGCGGAGC TCCAGGACGT CATTAATGAC 
ATGCAGTCCA AACTGCGGCT TGTCCCAGAC AAGCTGAAGA ATACGGAGCA TGACTTGAAG 
AGGAAGGAGC GCAGACGTGA TGAAATGATG ACACTGAAGC CCATCAGGCA GTCCTTAGTG 
GAGCTCCAAG AGAAAGAGCT ACCAGAGTTA AGAAATCAAC TCCAGCGTGT GAATCGGGAC 
ATTGAGAAAC TGAAAGGAGA CATCGAGGAG CAGGAGACAT TGCTGTGCAC GCTGGTGTCT 
GAGGAGGAGA CAGCCAAAGC CTGTCTGCAG GACATCTCTC TCATGGATCG CTTTCAGCTT 
GATCTAAAGG ATGTAGAGAG GAAGATTGCT CAACATGCAG CCAAGTTGCA GGGAGTTGAC 
CTCAGTCGCA CCATGCAGCA GGTCAGCCAG GAGAAACAGG AGACTCAACA CCGCCTGGAC 
ACTACTTCCA GTAAGATCGA GCTAAAGAGG AAACTAATTC AGGACCAGCA GGAGCAGATC 
CAGGCCTTGA GGAGCAGTGT GAACGAGATT CGTGGAGAAA AGCTGCAGAT CTCCAGCAAC 
ATGCAGAAGC GTCAGCAGTT GGAGGAGCAG TGTGTGGAGT TCTCCACCGA GATCCAGACA 
CTCCACAGGG ACATTCGAGA TGCAAAGGAG CAGGCCTCTC CTCTAGCTGC TACTTTGGAG 
AAGCTCCAGC AGGAGAAACA GGACCTCGTT GAGCGCAGGA GGAAAAAACA GGAGGAAGGA 
CAGGAAAAGA TAAATGCGAT TAAGGAAAAA ATGAAAAACA TCACCCTCTT TGAAAAAGAG 
ATCACCAAGT ACATTGAAGA GGGCAAAGAC AGCTACAAAG AGCAAAAAGA AACTGAACTA 
CAGGAGATGG ACAAACAACT ACATGAGGCC GAAAAACAGC GGGAGAAGAC CAATAAAGAC 
ATGGGCAACA TCCGACAAGA CATTGACACT CAGAAGGTTC AAGAGCGCTG GCTGCAGGAC 
AACTTGACTC TACGAAAGCG CGTGGAGGAG CTGAAAGAGG TGTCCAGAAA ACGAGAGGCC 
TTAATTAAGG AAATGGGCAA CATGCAAGTC CTGCAGCTGC GCAATGAGCG ACGAGAAGTA 
GAGCGAAAGT TGGAGGACCT AAAAAAGAAC CGCAGTGTGG CGCTTGGTCG ACAGAAAGGC 
TACGAGGATG AGATCCTCCG CTTTCGTAAA GAACTCAATG AGGATCAATA TTGCCGGGCC 
GAGGATCTCT ACAGAGACAA AATGATTGTG ATGAGAACAA CAGAGCTGGC CAATAAAGAC 
CTGGACATCT ACTATAAAGC CCTAGACCAA ACAATAATGA GGTTTCACAG CATGAAGATG 
GAGGAGATTA ATAAAATCAT CAGAGATTTG TGGCGGAGCA CATACAGAGG ACAAGACATC 
GAGTATGTAG AGATTCGCTC TGATGTGGAT GAGAATGCAT CTGCTGGTGT GAAGAGAAGG 
ACCTATAACT ATAGAGTGGT AATGGTGAAA GGAGACGCAG CCTTGGACAT GCGGGGTCGA 
TGCAGTGCTG GACAAAAGGT TCTCGCCTCT CTTATAATCC GATTGGCTCT AGCTGAGACA 
TTCTGCCTGA ACTGTGGGAT TCTGGCACTG GATGAGCCCA CTACCAACTT AGACAGGGAG 
AATATAGAGT CTTTGGCACA TGCACTTGTG GAAATTATCA AAAGTCGCTC TCGTCAGCGC 
AACTTCCAGC TGCTTGTCAT CACTCATGAC GAGGATTTCG TGGAGCTCCT GGGAAGATCC 
AACTATGTGG AGCATTTCTA CAGGATCAGG AAGAACCAGG ATCAGTGCTC AGAGATCAGC 
AAATGCAGTA TCAACAGCCT CAACTCCTAC CTGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_696859.4
CDS	96..4034
Translation	MSKIEKMSILGVRSFGVEDKDKQVISFFSPLTVLVGPNGAGKTTIIECLKYITSGDFPPGSKGNTFVHDPKDAHETDVRAQIRLQFRDVNGDAVAVQRSMQCTQKGKKTEFKTLEGVITRIKHGEKVSLSSKCAEIDREMISSLGVSRAVLNHVIFCHQEESNWPLSEGKALKQKFDEIFSATRYIKVLETLRTLRQKQTNTVKSCQMELKYLKQNKDKAQEIRELLSTKETQLASSKESVNRIEGQIDPLERRLNDIESSLGKVMKLDNDIKALDSRKKQMEDDNRELEEKMEQVFQGSDDQLQDMYQNHQRTVKEKEKRLVECQRELERAGRECQRMNRIKSELLVEQGRLQLEADRHTQNIKKRDTQVKTLASFLELEGYDRTPLSERQLQSFYRQIKERLDQDSEALNQTMHDMQQKETQKQHNIDDLRDKKTGLERTIELKKDLQAKKQQELKNIKSDLQKLEGSSNRLQELDTELQKAERELDNAVQACTVDSLKVEVTELLKEKAQLDQAQRKLDQEMEMLNTHTTARAQMDMMKKTKMDKEEQVRKIKSRHNEELVSLLGHFPNKKELEDWIYSKSREIKSTREQITKMNKELASGEQKKSHYTAEIKRKEEQLAKYEERLFNVCGSQDFQSDLSKLEDELEKCSKQRAMLAGATAVYSQFISQLTEEGDPCCPVCQRVFPSEAELQDVINDMQSKLRLVPDKLKNTEHDLKRKERRRDEMMTLKPIRQSLVELQEKELPELRNQLQRVNRDIEKLKGDIEEQETLLCTLVSEEETAKACLQDISLMDRFQLDLKDVERKIAQHAAKLQGVDLSRTMQQVSQEKQETQHRLDTTSSKIELKRKLIQDQQEQIQALRSSVNEIRGEKLQISSNMQKRQQLEEQCVEFSTEIQTLHRDIRDAKEQASPLAATLEKLQQEKQDLVERRRKKQEEGQEKINAIKEKMKNITLFEKEITKYIEEGKDSYKEQKETELQEMDKQLHEAEKQREKTNKDMGNIRQDIDTQKVQERWLQDNLTLRKRVEELKEVSRKREALIKEMGNMQVLQLRNERREVERKLEDLKKNRSVALGRQKGYEDEILRFRKELNEDQYCRAEDLYRDKMIVMRTTELANKDLDIYYKALDQTIMRFHSMKMEEINKIIRDLWRSTYRGQDIEYVEIRSDVDENASAGVKRRTYNYRVVMVKGDAALDMRGRCSAGQKVLASLIIRLALAETFCLNCGILALDEPTTNLDRENIESLAHALVEIIKSRSRQRNFQLLVITHDEDFVELLGRSNYVEHFYRIRKNQDQCSEISKCSINSLNSYLH

Target ORF information:

RefSeq Version	XM_691767.7
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio RAD50 homolog, double strand break repair protein (rad50), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_691767.7

ORF Insert Sequence:

ATGTCAAAGA TTGAGAAGAT GAGTATTCTG GGAGTGAGGA GTTTTGGAGT GGAGGATAAA 
GATAAACAAG TGATCTCCTT CTTCAGCCCT CTCACGGTGC TTGTGGGACC TAATGGAGCT 
GGCAAAACGA CCATCATTGA GTGTCTCAAG TACATCACAT CAGGAGACTT TCCACCAGGA 
AGTAAAGGAA ACACTTTTGT CCATGATCCC AAAGATGCTC ATGAGACTGA TGTACGGGCA 
CAGATCAGAC TCCAGTTTAG AGATGTGAAT GGAGATGCTG TGGCTGTGCA AAGATCTATG 
CAGTGCACAC AGAAAGGCAA GAAAACAGAA TTCAAAACGC TTGAGGGCGT CATTACGAGA 
ATAAAGCATG GTGAGAAAGT AAGCCTGAGC TCTAAATGTG CTGAGATAGA CCGTGAGATG 
ATCTCTTCCC TGGGCGTCTC CAGAGCTGTT CTCAACCATG TCATCTTTTG CCACCAAGAA 
GAGTCCAACT GGCCTCTCAG TGAAGGGAAA GCCCTCAAGC AGAAGTTTGA TGAGATTTTC 
TCAGCCACAA GGTACATTAA GGTGCTGGAG ACCTTGCGGA CGCTGAGGCA GAAGCAGACC 
AACACTGTTA AATCCTGCCA GATGGAGCTC AAGTACCTGA AACAGAACAA AGATAAAGCC 
CAAGAGATTC GAGAGCTGCT TTCCACCAAA GAAACTCAGC TAGCTTCCTC CAAAGAGAGT 
GTCAATCGCA TCGAGGGTCA GATTGACCCT CTGGAGAGAC GACTAAATGA CATAGAAAGC 
AGCCTCGGCA AAGTGATGAA ATTGGACAAC GACATCAAAG CTCTGGACAG TAGGAAGAAA 
CAGATGGAGG ATGATAACCG GGAGCTAGAG GAAAAGATGG AACAGGTGTT TCAGGGCTCA 
GATGATCAGC TACAGGACAT GTATCAGAAT CACCAGCGCA CCGTGAAAGA GAAGGAAAAA 
AGACTTGTAG AGTGCCAACG TGAGCTTGAA AGGGCTGGTC GAGAATGCCA GAGGATGAAC 
CGCATCAAGT CTGAGCTGCT AGTGGAGCAA GGTCGTCTGC AGTTGGAGGC AGATAGACAC 
ACACAGAACA TTAAAAAGAG AGACACCCAG GTAAAGACTT TGGCGTCTTT CCTGGAGTTG 
GAGGGCTATG ATCGAACGCC ACTTAGTGAG CGACAACTCC AAAGCTTTTA CAGACAAATC 
AAAGAAAGAC TGGACCAGGA CTCGGAAGCC CTCAACCAAA CTATGCATGA CATGCAGCAG 
AAAGAGACCC AGAAACAGCA CAACATTGAT GATCTCCGGG ATAAAAAGAC AGGTCTAGAG 
AGAACCATTG AGCTAAAGAA AGACCTACAG GCCAAAAAGC AACAAGAACT GAAGAATATC 
AAGTCTGACC TTCAAAAACT GGAGGGCTCC TCCAACAGAC TGCAAGAGCT GGACACTGAG 
CTTCAGAAAG CGGAGCGTGA GCTTGATAAT GCTGTGCAGG CCTGTACAGT GGACAGTCTG 
AAGGTCGAGG TAACTGAACT ACTGAAGGAA AAGGCTCAAC TGGATCAGGC ACAGCGCAAA 
CTGGACCAAG AGATGGAAAT GCTCAACACA CACACCACGG CACGTGCACA GATGGACATG 
ATGAAGAAAA CCAAGATGGA CAAAGAGGAG CAGGTGAGGA AGATCAAGTC GAGACATAAT 
GAAGAGCTGG TGTCTCTGCT GGGACACTTT CCAAACAAGA AAGAGCTGGA GGACTGGATC 
TACTCCAAAT CCAGAGAGAT CAAATCCACT AGAGAGCAGA TCACCAAAAT GAATAAAGAG 
CTTGCATCTG GAGAACAGAA GAAGAGTCAT TACACTGCAG AAATCAAACG AAAAGAAGAG 
CAGCTGGCCA AATATGAAGA GAGACTTTTT AATGTTTGTG GAAGCCAGGA TTTCCAGTCA 
GACTTAAGCA AACTGGAGGA TGAGTTGGAA AAGTGCTCCA AACAGAGAGC CATGTTGGCA 
GGTGCCACAG CTGTGTACAG TCAGTTCATC AGTCAGCTGA CGGAGGAAGG TGACCCATGC 
TGCCCCGTAT GTCAGCGCGT GTTCCCATCA GAGGCGGAGC TCCAGGACGT CATTAATGAC 
ATGCAGTCCA AACTGCGGCT TGTCCCAGAC AAGCTGAAGA ATACGGAGCA TGACTTGAAG 
AGGAAGGAGC GCAGACGTGA TGAAATGATG ACACTGAAGC CCATCAGGCA GTCCTTAGTG 
GAGCTCCAAG AGAAAGAGCT ACCAGAGTTA AGAAATCAAC TCCAGCGTGT GAATCGGGAC 
ATTGAGAAAC TGAAAGGAGA CATCGAGGAG CAGGAGACAT TGCTGTGCAC GCTGGTGTCT 
GAGGAGGAGA CAGCCAAAGC CTGTCTGCAG GACATCTCTC TCATGGATCG CTTTCAGCTT 
GATCTAAAGG ATGTAGAGAG GAAGATTGCT CAACATGCAG CCAAGTTGCA GGGAGTTGAC 
CTCAGTCGCA CCATGCAGCA GGTCAGCCAG GAGAAACAGG AGACTCAACA CCGCCTGGAC 
ACTACTTCCA GTAAGATCGA GCTAAAGAGG AAACTAATTC AGGACCAGCA GGAGCAGATC 
CAGGCCTTGA GGAGCAGTGT GAACGAGATT CGTGGAGAAA AGCTGCAGAT CTCCAGCAAC 
ATGCAGAAGC GTCAGCAGTT GGAGGAGCAG TGTGTGGAGT TCTCCACCGA GATCCAGACA 
CTCCACAGGG ACATTCGAGA TGCAAAGGAG CAGGCCTCTC CTCTAGCTGC TACTTTGGAG 
AAGCTCCAGC AGGAGAAACA GGACCTCGTT GAGCGCAGGA GGAAAAAACA GGAGGAAGGA 
CAGGAAAAGA TAAATGCGAT TAAGGAAAAA ATGAAAAACA TCACCCTCTT TGAAAAAGAG 
ATCACCAAGT ACATTGAAGA GGGCAAAGAC AGCTACAAAG AGCAAAAAGA AACTGAACTA 
CAGGAGATGG ACAAACAACT ACATGAGGCC GAAAAACAGC GGGAGAAGAC CAATAAAGAC 
ATGGGCAACA TCCGACAAGA CATTGACACT CAGAAGGTTC AAGAGCGCTG GCTGCAGGAC 
AACTTGACTC TACGAAAGCG CGTGGAGGAG CTGAAAGAGG TGTCCAGAAA ACGAGAGGCC 
TTAATTAAGG AAATGGGCAA CATGCAAGTC CTGCAGCTGC GCAATGAGCG ACGAGAAGTA 
GAGCGAAAGT TGGAGGACCT AAAAAAGAAC CGCAGTGTGG CGCTTGGTCG ACAGAAAGGC 
TACGAGGATG AGATCCTCCG CTTTCGTAAA GAACTCAATG AGGATCAATA TTGCCGGGCC 
GAGGATCTCT ACAGAGACAA AATGATTGTG ATGAGAACAA CAGAGCTGGC CAATAAAGAC 
CTGGACATCT ACTATAAAGC CCTAGACCAA ACAATAATGA GGTTTCACAG CATGAAGATG 
GAGGAGATTA ATAAAATCAT CAGAGATTTG TGGCGGAGCA CATACAGAGG ACAAGACATC 
GAGTATGTAG AGATTCGCTC TGATGTGGAT GAGAATGCAT CTGCTGGTGT GAAGAGAAGG 
ACCTATAACT ATAGAGTGGT AATGGTGAAA GGAGACGCAG CCTTGGACAT GCGGGGTCGA 
TGCAGTGCTG GACAAAAGGT TCTCGCCTCT CTTATAATCC GATTGGCTCT AGCTGAGACA 
TTCTGCCTGA ACTGTGGGAT TCTGGCACTG GATGAGCCCA CTACCAACTT AGACAGGGAG 
AATATAGAGT CTTTGGCACA TGCACTTGTG GAAATTATCA AAAGTCGCTC TCGTCAGCGC 
AACTTCCAGC TGCTTGTCAT CACTCATGAC GAGGATTTCG TGGAGCTCCT GGGAAGATCC 
AACTATGTGG AGCATTTCTA CAGGATCAGG AAGAACCAGG ATCAGTGCTC AGAGATCAGC 
AAATGCAGTA TCAACAGCCT CAACTCCTAC CTGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rad50 ( XM_005167938.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005167995.1 Danio rerio RAD50 homolog, double strand break repair protein (rad50), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa15615
Clone ID Related Accession (Same CDS sequence)	XM_691767.7 , XM_005167938.3 , XM_005167938.4 , XM_691767.8
Accession Version	XM_005167938.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	DNA repair protein RAD50
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005167938.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGA TTGAGAAGAT GAGTATTCTG GGAGTGAGGA GTTTTGGAGT GGAGGATAAA 
GATAAACAAG TGATCTCCTT CTTCAGCCCT CTCACGGTGC TTGTGGGACC TAATGGAGCT 
GGCAAAACGA CCATCATTGA GTGTCTCAAG TACATCACAT CAGGAGACTT TCCACCAGGA 
AGTAAAGGAA ACACTTTTGT CCATGATCCC AAAGATGCTC ATGAGACTGA TGTACGGGCA 
CAGATCAGAC TCCAGTTTAG AGATGTGAAT GGAGATGCTG TGGCTGTGCA AAGATCTATG 
CAGTGCACAC AGAAAGGCAA GAAAACAGAA TTCAAAACGC TTGAGGGCGT CATTACGAGA 
ATAAAGCATG GTGAGAAAGT AAGCCTGAGC TCTAAATGTG CTGAGATAGA CCGTGAGATG 
ATCTCTTCCC TGGGCGTCTC CAGAGCTGTT CTCAACCATG TCATCTTTTG CCACCAAGAA 
GAGTCCAACT GGCCTCTCAG TGAAGGGAAA GCCCTCAAGC AGAAGTTTGA TGAGATTTTC 
TCAGCCACAA GGTACATTAA GGTGCTGGAG ACCTTGCGGA CGCTGAGGCA GAAGCAGACC 
AACACTGTTA AATCCTGCCA GATGGAGCTC AAGTACCTGA AACAGAACAA AGATAAAGCC 
CAAGAGATTC GAGAGCTGCT TTCCACCAAA GAAACTCAGC TAGCTTCCTC CAAAGAGAGT 
GTCAATCGCA TCGAGGGTCA GATTGACCCT CTGGAGAGAC GACTAAATGA CATAGAAAGC 
AGCCTCGGCA AAGTGATGAA ATTGGACAAC GACATCAAAG CTCTGGACAG TAGGAAGAAA 
CAGATGGAGG ATGATAACCG GGAGCTAGAG GAAAAGATGG AACAGGTGTT TCAGGGCTCA 
GATGATCAGC TACAGGACAT GTATCAGAAT CACCAGCGCA CCGTGAAAGA GAAGGAAAAA 
AGACTTGTAG AGTGCCAACG TGAGCTTGAA AGGGCTGGTC GAGAATGCCA GAGGATGAAC 
CGCATCAAGT CTGAGCTGCT AGTGGAGCAA GGTCGTCTGC AGTTGGAGGC AGATAGACAC 
ACACAGAACA TTAAAAAGAG AGACACCCAG GTAAAGACTT TGGCGTCTTT CCTGGAGTTG 
GAGGGCTATG ATCGAACGCC ACTTAGTGAG CGACAACTCC AAAGCTTTTA CAGACAAATC 
AAAGAAAGAC TGGACCAGGA CTCGGAAGCC CTCAACCAAA CTATGCATGA CATGCAGCAG 
AAAGAGACCC AGAAACAGCA CAACATTGAT GATCTCCGGG ATAAAAAGAC AGGTCTAGAG 
AGAACCATTG AGCTAAAGAA AGACCTACAG GCCAAAAAGC AACAAGAACT GAAGAATATC 
AAGTCTGACC TTCAAAAACT GGAGGGCTCC TCCAACAGAC TGCAAGAGCT GGACACTGAG 
CTTCAGAAAG CGGAGCGTGA GCTTGATAAT GCTGTGCAGG CCTGTACAGT GGACAGTCTG 
AAGGTCGAGG TAACTGAACT ACTGAAGGAA AAGGCTCAAC TGGATCAGGC ACAGCGCAAA 
CTGGACCAAG AGATGGAAAT GCTCAACACA CACACCACGG CACGTGCACA GATGGACATG 
ATGAAGAAAA CCAAGATGGA CAAAGAGGAG CAGGTGAGGA AGATCAAGTC GAGACATAAT 
GAAGAGCTGG TGTCTCTGCT GGGACACTTT CCAAACAAGA AAGAGCTGGA GGACTGGATC 
TACTCCAAAT CCAGAGAGAT CAAATCCACT AGAGAGCAGA TCACCAAAAT GAATAAAGAG 
CTTGCATCTG GAGAACAGAA GAAGAGTCAT TACACTGCAG AAATCAAACG AAAAGAAGAG 
CAGCTGGCCA AATATGAAGA GAGACTTTTT AATGTTTGTG GAAGCCAGGA TTTCCAGTCA 
GACTTAAGCA AACTGGAGGA TGAGTTGGAA AAGTGCTCCA AACAGAGAGC CATGTTGGCA 
GGTGCCACAG CTGTGTACAG TCAGTTCATC AGTCAGCTGA CGGAGGAAGG TGACCCATGC 
TGCCCCGTAT GTCAGCGCGT GTTCCCATCA GAGGCGGAGC TCCAGGACGT CATTAATGAC 
ATGCAGTCCA AACTGCGGCT TGTCCCAGAC AAGCTGAAGA ATACGGAGCA TGACTTGAAG 
AGGAAGGAGC GCAGACGTGA TGAAATGATG ACACTGAAGC CCATCAGGCA GTCCTTAGTG 
GAGCTCCAAG AGAAAGAGCT ACCAGAGTTA AGAAATCAAC TCCAGCGTGT GAATCGGGAC 
ATTGAGAAAC TGAAAGGAGA CATCGAGGAG CAGGAGACAT TGCTGTGCAC GCTGGTGTCT 
GAGGAGGAGA CAGCCAAAGC CTGTCTGCAG GACATCTCTC TCATGGATCG CTTTCAGCTT 
GATCTAAAGG ATGTAGAGAG GAAGATTGCT CAACATGCAG CCAAGTTGCA GGGAGTTGAC 
CTCAGTCGCA CCATGCAGCA GGTCAGCCAG GAGAAACAGG AGACTCAACA CCGCCTGGAC 
ACTACTTCCA GTAAGATCGA GCTAAAGAGG AAACTAATTC AGGACCAGCA GGAGCAGATC 
CAGGCCTTGA GGAGCAGTGT GAACGAGATT CGTGGAGAAA AGCTGCAGAT CTCCAGCAAC 
ATGCAGAAGC GTCAGCAGTT GGAGGAGCAG TGTGTGGAGT TCTCCACCGA GATCCAGACA 
CTCCACAGGG ACATTCGAGA TGCAAAGGAG CAGGCCTCTC CTCTAGCTGC TACTTTGGAG 
AAGCTCCAGC AGGAGAAACA GGACCTCGTT GAGCGCAGGA GGAAAAAACA GGAGGAAGGA 
CAGGAAAAGA TAAATGCGAT TAAGGAAAAA ATGAAAAACA TCACCCTCTT TGAAAAAGAG 
ATCACCAAGT ACATTGAAGA GGGCAAAGAC AGCTACAAAG AGCAAAAAGA AACTGAACTA 
CAGGAGATGG ACAAACAACT ACATGAGGCC GAAAAACAGC GGGAGAAGAC CAATAAAGAC 
ATGGGCAACA TCCGACAAGA CATTGACACT CAGAAGGTTC AAGAGCGCTG GCTGCAGGAC 
AACTTGACTC TACGAAAGCG CGTGGAGGAG CTGAAAGAGG TGTCCAGAAA ACGAGAGGCC 
TTAATTAAGG AAATGGGCAA CATGCAAGTC CTGCAGCTGC GCAATGAGCG ACGAGAAGTA 
GAGCGAAAGT TGGAGGACCT AAAAAAGAAC CGCAGTGTGG CGCTTGGTCG ACAGAAAGGC 
TACGAGGATG AGATCCTCCG CTTTCGTAAA GAACTCAATG AGGATCAATA TTGCCGGGCC 
GAGGATCTCT ACAGAGACAA AATGATTGTG ATGAGAACAA CAGAGCTGGC CAATAAAGAC 
CTGGACATCT ACTATAAAGC CCTAGACCAA ACAATAATGA GGTTTCACAG CATGAAGATG 
GAGGAGATTA ATAAAATCAT CAGAGATTTG TGGCGGAGCA CATACAGAGG ACAAGACATC 
GAGTATGTAG AGATTCGCTC TGATGTGGAT GAGAATGCAT CTGCTGGTGT GAAGAGAAGG 
ACCTATAACT ATAGAGTGGT AATGGTGAAA GGAGACGCAG CCTTGGACAT GCGGGGTCGA 
TGCAGTGCTG GACAAAAGGT TCTCGCCTCT CTTATAATCC GATTGGCTCT AGCTGAGACA 
TTCTGCCTGA ACTGTGGGAT TCTGGCACTG GATGAGCCCA CTACCAACTT AGACAGGGAG 
AATATAGAGT CTTTGGCACA TGCACTTGTG GAAATTATCA AAAGTCGCTC TCGTCAGCGC 
AACTTCCAGC TGCTTGTCAT CACTCATGAC GAGGATTTCG TGGAGCTCCT GGGAAGATCC 
AACTATGTGG AGCATTTCTA CAGGATCAGG AAGAACCAGG ATCAGTGCTC AGAGATCAGC 
AAATGCAGTA TCAACAGCCT CAACTCCTAC CTGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167995.1
CDS	73..4011
Translation	MSKIEKMSILGVRSFGVEDKDKQVISFFSPLTVLVGPNGAGKTTIIECLKYITSGDFPPGSKGNTFVHDPKDAHETDVRAQIRLQFRDVNGDAVAVQRSMQCTQKGKKTEFKTLEGVITRIKHGEKVSLSSKCAEIDREMISSLGVSRAVLNHVIFCHQEESNWPLSEGKALKQKFDEIFSATRYIKVLETLRTLRQKQTNTVKSCQMELKYLKQNKDKAQEIRELLSTKETQLASSKESVNRIEGQIDPLERRLNDIESSLGKVMKLDNDIKALDSRKKQMEDDNRELEEKMEQVFQGSDDQLQDMYQNHQRTVKEKEKRLVECQRELERAGRECQRMNRIKSELLVEQGRLQLEADRHTQNIKKRDTQVKTLASFLELEGYDRTPLSERQLQSFYRQIKERLDQDSEALNQTMHDMQQKETQKQHNIDDLRDKKTGLERTIELKKDLQAKKQQELKNIKSDLQKLEGSSNRLQELDTELQKAERELDNAVQACTVDSLKVEVTELLKEKAQLDQAQRKLDQEMEMLNTHTTARAQMDMMKKTKMDKEEQVRKIKSRHNEELVSLLGHFPNKKELEDWIYSKSREIKSTREQITKMNKELASGEQKKSHYTAEIKRKEEQLAKYEERLFNVCGSQDFQSDLSKLEDELEKCSKQRAMLAGATAVYSQFISQLTEEGDPCCPVCQRVFPSEAELQDVINDMQSKLRLVPDKLKNTEHDLKRKERRRDEMMTLKPIRQSLVELQEKELPELRNQLQRVNRDIEKLKGDIEEQETLLCTLVSEEETAKACLQDISLMDRFQLDLKDVERKIAQHAAKLQGVDLSRTMQQVSQEKQETQHRLDTTSSKIELKRKLIQDQQEQIQALRSSVNEIRGEKLQISSNMQKRQQLEEQCVEFSTEIQTLHRDIRDAKEQASPLAATLEKLQQEKQDLVERRRKKQEEGQEKINAIKEKMKNITLFEKEITKYIEEGKDSYKEQKETELQEMDKQLHEAEKQREKTNKDMGNIRQDIDTQKVQERWLQDNLTLRKRVEELKEVSRKREALIKEMGNMQVLQLRNERREVERKLEDLKKNRSVALGRQKGYEDEILRFRKELNEDQYCRAEDLYRDKMIVMRTTELANKDLDIYYKALDQTIMRFHSMKMEEINKIIRDLWRSTYRGQDIEYVEIRSDVDENASAGVKRRTYNYRVVMVKGDAALDMRGRCSAGQKVLASLIIRLALAETFCLNCGILALDEPTTNLDRENIESLAHALVEIIKSRSRQRNFQLLVITHDEDFVELLGRSNYVEHFYRIRKNQDQCSEISKCSINSLNSYLH

Target ORF information:

RefSeq Version	XM_005167938.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio RAD50 homolog, double strand break repair protein (rad50), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167938.3

ORF Insert Sequence:

ATGTCAAAGA TTGAGAAGAT GAGTATTCTG GGAGTGAGGA GTTTTGGAGT GGAGGATAAA 
GATAAACAAG TGATCTCCTT CTTCAGCCCT CTCACGGTGC TTGTGGGACC TAATGGAGCT 
GGCAAAACGA CCATCATTGA GTGTCTCAAG TACATCACAT CAGGAGACTT TCCACCAGGA 
AGTAAAGGAA ACACTTTTGT CCATGATCCC AAAGATGCTC ATGAGACTGA TGTACGGGCA 
CAGATCAGAC TCCAGTTTAG AGATGTGAAT GGAGATGCTG TGGCTGTGCA AAGATCTATG 
CAGTGCACAC AGAAAGGCAA GAAAACAGAA TTCAAAACGC TTGAGGGCGT CATTACGAGA 
ATAAAGCATG GTGAGAAAGT AAGCCTGAGC TCTAAATGTG CTGAGATAGA CCGTGAGATG 
ATCTCTTCCC TGGGCGTCTC CAGAGCTGTT CTCAACCATG TCATCTTTTG CCACCAAGAA 
GAGTCCAACT GGCCTCTCAG TGAAGGGAAA GCCCTCAAGC AGAAGTTTGA TGAGATTTTC 
TCAGCCACAA GGTACATTAA GGTGCTGGAG ACCTTGCGGA CGCTGAGGCA GAAGCAGACC 
AACACTGTTA AATCCTGCCA GATGGAGCTC AAGTACCTGA AACAGAACAA AGATAAAGCC 
CAAGAGATTC GAGAGCTGCT TTCCACCAAA GAAACTCAGC TAGCTTCCTC CAAAGAGAGT 
GTCAATCGCA TCGAGGGTCA GATTGACCCT CTGGAGAGAC GACTAAATGA CATAGAAAGC 
AGCCTCGGCA AAGTGATGAA ATTGGACAAC GACATCAAAG CTCTGGACAG TAGGAAGAAA 
CAGATGGAGG ATGATAACCG GGAGCTAGAG GAAAAGATGG AACAGGTGTT TCAGGGCTCA 
GATGATCAGC TACAGGACAT GTATCAGAAT CACCAGCGCA CCGTGAAAGA GAAGGAAAAA 
AGACTTGTAG AGTGCCAACG TGAGCTTGAA AGGGCTGGTC GAGAATGCCA GAGGATGAAC 
CGCATCAAGT CTGAGCTGCT AGTGGAGCAA GGTCGTCTGC AGTTGGAGGC AGATAGACAC 
ACACAGAACA TTAAAAAGAG AGACACCCAG GTAAAGACTT TGGCGTCTTT CCTGGAGTTG 
GAGGGCTATG ATCGAACGCC ACTTAGTGAG CGACAACTCC AAAGCTTTTA CAGACAAATC 
AAAGAAAGAC TGGACCAGGA CTCGGAAGCC CTCAACCAAA CTATGCATGA CATGCAGCAG 
AAAGAGACCC AGAAACAGCA CAACATTGAT GATCTCCGGG ATAAAAAGAC AGGTCTAGAG 
AGAACCATTG AGCTAAAGAA AGACCTACAG GCCAAAAAGC AACAAGAACT GAAGAATATC 
AAGTCTGACC TTCAAAAACT GGAGGGCTCC TCCAACAGAC TGCAAGAGCT GGACACTGAG 
CTTCAGAAAG CGGAGCGTGA GCTTGATAAT GCTGTGCAGG CCTGTACAGT GGACAGTCTG 
AAGGTCGAGG TAACTGAACT ACTGAAGGAA AAGGCTCAAC TGGATCAGGC ACAGCGCAAA 
CTGGACCAAG AGATGGAAAT GCTCAACACA CACACCACGG CACGTGCACA GATGGACATG 
ATGAAGAAAA CCAAGATGGA CAAAGAGGAG CAGGTGAGGA AGATCAAGTC GAGACATAAT 
GAAGAGCTGG TGTCTCTGCT GGGACACTTT CCAAACAAGA AAGAGCTGGA GGACTGGATC 
TACTCCAAAT CCAGAGAGAT CAAATCCACT AGAGAGCAGA TCACCAAAAT GAATAAAGAG 
CTTGCATCTG GAGAACAGAA GAAGAGTCAT TACACTGCAG AAATCAAACG AAAAGAAGAG 
CAGCTGGCCA AATATGAAGA GAGACTTTTT AATGTTTGTG GAAGCCAGGA TTTCCAGTCA 
GACTTAAGCA AACTGGAGGA TGAGTTGGAA AAGTGCTCCA AACAGAGAGC CATGTTGGCA 
GGTGCCACAG CTGTGTACAG TCAGTTCATC AGTCAGCTGA CGGAGGAAGG TGACCCATGC 
TGCCCCGTAT GTCAGCGCGT GTTCCCATCA GAGGCGGAGC TCCAGGACGT CATTAATGAC 
ATGCAGTCCA AACTGCGGCT TGTCCCAGAC AAGCTGAAGA ATACGGAGCA TGACTTGAAG 
AGGAAGGAGC GCAGACGTGA TGAAATGATG ACACTGAAGC CCATCAGGCA GTCCTTAGTG 
GAGCTCCAAG AGAAAGAGCT ACCAGAGTTA AGAAATCAAC TCCAGCGTGT GAATCGGGAC 
ATTGAGAAAC TGAAAGGAGA CATCGAGGAG CAGGAGACAT TGCTGTGCAC GCTGGTGTCT 
GAGGAGGAGA CAGCCAAAGC CTGTCTGCAG GACATCTCTC TCATGGATCG CTTTCAGCTT 
GATCTAAAGG ATGTAGAGAG GAAGATTGCT CAACATGCAG CCAAGTTGCA GGGAGTTGAC 
CTCAGTCGCA CCATGCAGCA GGTCAGCCAG GAGAAACAGG AGACTCAACA CCGCCTGGAC 
ACTACTTCCA GTAAGATCGA GCTAAAGAGG AAACTAATTC AGGACCAGCA GGAGCAGATC 
CAGGCCTTGA GGAGCAGTGT GAACGAGATT CGTGGAGAAA AGCTGCAGAT CTCCAGCAAC 
ATGCAGAAGC GTCAGCAGTT GGAGGAGCAG TGTGTGGAGT TCTCCACCGA GATCCAGACA 
CTCCACAGGG ACATTCGAGA TGCAAAGGAG CAGGCCTCTC CTCTAGCTGC TACTTTGGAG 
AAGCTCCAGC AGGAGAAACA GGACCTCGTT GAGCGCAGGA GGAAAAAACA GGAGGAAGGA 
CAGGAAAAGA TAAATGCGAT TAAGGAAAAA ATGAAAAACA TCACCCTCTT TGAAAAAGAG 
ATCACCAAGT ACATTGAAGA GGGCAAAGAC AGCTACAAAG AGCAAAAAGA AACTGAACTA 
CAGGAGATGG ACAAACAACT ACATGAGGCC GAAAAACAGC GGGAGAAGAC CAATAAAGAC 
ATGGGCAACA TCCGACAAGA CATTGACACT CAGAAGGTTC AAGAGCGCTG GCTGCAGGAC 
AACTTGACTC TACGAAAGCG CGTGGAGGAG CTGAAAGAGG TGTCCAGAAA ACGAGAGGCC 
TTAATTAAGG AAATGGGCAA CATGCAAGTC CTGCAGCTGC GCAATGAGCG ACGAGAAGTA 
GAGCGAAAGT TGGAGGACCT AAAAAAGAAC CGCAGTGTGG CGCTTGGTCG ACAGAAAGGC 
TACGAGGATG AGATCCTCCG CTTTCGTAAA GAACTCAATG AGGATCAATA TTGCCGGGCC 
GAGGATCTCT ACAGAGACAA AATGATTGTG ATGAGAACAA CAGAGCTGGC CAATAAAGAC 
CTGGACATCT ACTATAAAGC CCTAGACCAA ACAATAATGA GGTTTCACAG CATGAAGATG 
GAGGAGATTA ATAAAATCAT CAGAGATTTG TGGCGGAGCA CATACAGAGG ACAAGACATC 
GAGTATGTAG AGATTCGCTC TGATGTGGAT GAGAATGCAT CTGCTGGTGT GAAGAGAAGG 
ACCTATAACT ATAGAGTGGT AATGGTGAAA GGAGACGCAG CCTTGGACAT GCGGGGTCGA 
TGCAGTGCTG GACAAAAGGT TCTCGCCTCT CTTATAATCC GATTGGCTCT AGCTGAGACA 
TTCTGCCTGA ACTGTGGGAT TCTGGCACTG GATGAGCCCA CTACCAACTT AGACAGGGAG 
AATATAGAGT CTTTGGCACA TGCACTTGTG GAAATTATCA AAAGTCGCTC TCGTCAGCGC 
AACTTCCAGC TGCTTGTCAT CACTCATGAC GAGGATTTCG TGGAGCTCCT GGGAAGATCC 
AACTATGTGG AGCATTTCTA CAGGATCAGG AAGAACCAGG ATCAGTGCTC AGAGATCAGC 
AAATGCAGTA TCAACAGCCT CAACTCCTAC CTGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rad50 ( XM_005167938.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005167995.1 Danio rerio RAD50 homolog, double strand break repair protein (rad50), transcript variant X1, mRNA.

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CloneID	ODa15615
Clone ID Related Accession (Same CDS sequence)	XM_691767.7 , XM_005167938.3 , XM_005167938.4 , XM_691767.8
Accession Version	XM_005167938.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	DNA repair protein RAD50
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005167938.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGA TTGAGAAGAT GAGTATTCTG GGAGTGAGGA GTTTTGGAGT GGAGGATAAA 
GATAAACAAG TGATCTCCTT CTTCAGCCCT CTCACGGTGC TTGTGGGACC TAATGGAGCT 
GGCAAAACGA CCATCATTGA GTGTCTCAAG TACATCACAT CAGGAGACTT TCCACCAGGA 
AGTAAAGGAA ACACTTTTGT CCATGATCCC AAAGATGCTC ATGAGACTGA TGTACGGGCA 
CAGATCAGAC TCCAGTTTAG AGATGTGAAT GGAGATGCTG TGGCTGTGCA AAGATCTATG 
CAGTGCACAC AGAAAGGCAA GAAAACAGAA TTCAAAACGC TTGAGGGCGT CATTACGAGA 
ATAAAGCATG GTGAGAAAGT AAGCCTGAGC TCTAAATGTG CTGAGATAGA CCGTGAGATG 
ATCTCTTCCC TGGGCGTCTC CAGAGCTGTT CTCAACCATG TCATCTTTTG CCACCAAGAA 
GAGTCCAACT GGCCTCTCAG TGAAGGGAAA GCCCTCAAGC AGAAGTTTGA TGAGATTTTC 
TCAGCCACAA GGTACATTAA GGTGCTGGAG ACCTTGCGGA CGCTGAGGCA GAAGCAGACC 
AACACTGTTA AATCCTGCCA GATGGAGCTC AAGTACCTGA AACAGAACAA AGATAAAGCC 
CAAGAGATTC GAGAGCTGCT TTCCACCAAA GAAACTCAGC TAGCTTCCTC CAAAGAGAGT 
GTCAATCGCA TCGAGGGTCA GATTGACCCT CTGGAGAGAC GACTAAATGA CATAGAAAGC 
AGCCTCGGCA AAGTGATGAA ATTGGACAAC GACATCAAAG CTCTGGACAG TAGGAAGAAA 
CAGATGGAGG ATGATAACCG GGAGCTAGAG GAAAAGATGG AACAGGTGTT TCAGGGCTCA 
GATGATCAGC TACAGGACAT GTATCAGAAT CACCAGCGCA CCGTGAAAGA GAAGGAAAAA 
AGACTTGTAG AGTGCCAACG TGAGCTTGAA AGGGCTGGTC GAGAATGCCA GAGGATGAAC 
CGCATCAAGT CTGAGCTGCT AGTGGAGCAA GGTCGTCTGC AGTTGGAGGC AGATAGACAC 
ACACAGAACA TTAAAAAGAG AGACACCCAG GTAAAGACTT TGGCGTCTTT CCTGGAGTTG 
GAGGGCTATG ATCGAACGCC ACTTAGTGAG CGACAACTCC AAAGCTTTTA CAGACAAATC 
AAAGAAAGAC TGGACCAGGA CTCGGAAGCC CTCAACCAAA CTATGCATGA CATGCAGCAG 
AAAGAGACCC AGAAACAGCA CAACATTGAT GATCTCCGGG ATAAAAAGAC AGGTCTAGAG 
AGAACCATTG AGCTAAAGAA AGACCTACAG GCCAAAAAGC AACAAGAACT GAAGAATATC 
AAGTCTGACC TTCAAAAACT GGAGGGCTCC TCCAACAGAC TGCAAGAGCT GGACACTGAG 
CTTCAGAAAG CGGAGCGTGA GCTTGATAAT GCTGTGCAGG CCTGTACAGT GGACAGTCTG 
AAGGTCGAGG TAACTGAACT ACTGAAGGAA AAGGCTCAAC TGGATCAGGC ACAGCGCAAA 
CTGGACCAAG AGATGGAAAT GCTCAACACA CACACCACGG CACGTGCACA GATGGACATG 
ATGAAGAAAA CCAAGATGGA CAAAGAGGAG CAGGTGAGGA AGATCAAGTC GAGACATAAT 
GAAGAGCTGG TGTCTCTGCT GGGACACTTT CCAAACAAGA AAGAGCTGGA GGACTGGATC 
TACTCCAAAT CCAGAGAGAT CAAATCCACT AGAGAGCAGA TCACCAAAAT GAATAAAGAG 
CTTGCATCTG GAGAACAGAA GAAGAGTCAT TACACTGCAG AAATCAAACG AAAAGAAGAG 
CAGCTGGCCA AATATGAAGA GAGACTTTTT AATGTTTGTG GAAGCCAGGA TTTCCAGTCA 
GACTTAAGCA AACTGGAGGA TGAGTTGGAA AAGTGCTCCA AACAGAGAGC CATGTTGGCA 
GGTGCCACAG CTGTGTACAG TCAGTTCATC AGTCAGCTGA CGGAGGAAGG TGACCCATGC 
TGCCCCGTAT GTCAGCGCGT GTTCCCATCA GAGGCGGAGC TCCAGGACGT CATTAATGAC 
ATGCAGTCCA AACTGCGGCT TGTCCCAGAC AAGCTGAAGA ATACGGAGCA TGACTTGAAG 
AGGAAGGAGC GCAGACGTGA TGAAATGATG ACACTGAAGC CCATCAGGCA GTCCTTAGTG 
GAGCTCCAAG AGAAAGAGCT ACCAGAGTTA AGAAATCAAC TCCAGCGTGT GAATCGGGAC 
ATTGAGAAAC TGAAAGGAGA CATCGAGGAG CAGGAGACAT TGCTGTGCAC GCTGGTGTCT 
GAGGAGGAGA CAGCCAAAGC CTGTCTGCAG GACATCTCTC TCATGGATCG CTTTCAGCTT 
GATCTAAAGG ATGTAGAGAG GAAGATTGCT CAACATGCAG CCAAGTTGCA GGGAGTTGAC 
CTCAGTCGCA CCATGCAGCA GGTCAGCCAG GAGAAACAGG AGACTCAACA CCGCCTGGAC 
ACTACTTCCA GTAAGATCGA GCTAAAGAGG AAACTAATTC AGGACCAGCA GGAGCAGATC 
CAGGCCTTGA GGAGCAGTGT GAACGAGATT CGTGGAGAAA AGCTGCAGAT CTCCAGCAAC 
ATGCAGAAGC GTCAGCAGTT GGAGGAGCAG TGTGTGGAGT TCTCCACCGA GATCCAGACA 
CTCCACAGGG ACATTCGAGA TGCAAAGGAG CAGGCCTCTC CTCTAGCTGC TACTTTGGAG 
AAGCTCCAGC AGGAGAAACA GGACCTCGTT GAGCGCAGGA GGAAAAAACA GGAGGAAGGA 
CAGGAAAAGA TAAATGCGAT TAAGGAAAAA ATGAAAAACA TCACCCTCTT TGAAAAAGAG 
ATCACCAAGT ACATTGAAGA GGGCAAAGAC AGCTACAAAG AGCAAAAAGA AACTGAACTA 
CAGGAGATGG ACAAACAACT ACATGAGGCC GAAAAACAGC GGGAGAAGAC CAATAAAGAC 
ATGGGCAACA TCCGACAAGA CATTGACACT CAGAAGGTTC AAGAGCGCTG GCTGCAGGAC 
AACTTGACTC TACGAAAGCG CGTGGAGGAG CTGAAAGAGG TGTCCAGAAA ACGAGAGGCC 
TTAATTAAGG AAATGGGCAA CATGCAAGTC CTGCAGCTGC GCAATGAGCG ACGAGAAGTA 
GAGCGAAAGT TGGAGGACCT AAAAAAGAAC CGCAGTGTGG CGCTTGGTCG ACAGAAAGGC 
TACGAGGATG AGATCCTCCG CTTTCGTAAA GAACTCAATG AGGATCAATA TTGCCGGGCC 
GAGGATCTCT ACAGAGACAA AATGATTGTG ATGAGAACAA CAGAGCTGGC CAATAAAGAC 
CTGGACATCT ACTATAAAGC CCTAGACCAA ACAATAATGA GGTTTCACAG CATGAAGATG 
GAGGAGATTA ATAAAATCAT CAGAGATTTG TGGCGGAGCA CATACAGAGG ACAAGACATC 
GAGTATGTAG AGATTCGCTC TGATGTGGAT GAGAATGCAT CTGCTGGTGT GAAGAGAAGG 
ACCTATAACT ATAGAGTGGT AATGGTGAAA GGAGACGCAG CCTTGGACAT GCGGGGTCGA 
TGCAGTGCTG GACAAAAGGT TCTCGCCTCT CTTATAATCC GATTGGCTCT AGCTGAGACA 
TTCTGCCTGA ACTGTGGGAT TCTGGCACTG GATGAGCCCA CTACCAACTT AGACAGGGAG 
AATATAGAGT CTTTGGCACA TGCACTTGTG GAAATTATCA AAAGTCGCTC TCGTCAGCGC 
AACTTCCAGC TGCTTGTCAT CACTCATGAC GAGGATTTCG TGGAGCTCCT GGGAAGATCC 
AACTATGTGG AGCATTTCTA CAGGATCAGG AAGAACCAGG ATCAGTGCTC AGAGATCAGC 
AAATGCAGTA TCAACAGCCT CAACTCCTAC CTGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167995.1
CDS	86..4024
Translation	MSKIEKMSILGVRSFGVEDKDKQVISFFSPLTVLVGPNGAGKTTIIECLKYITSGDFPPGSKGNTFVHDPKDAHETDVRAQIRLQFRDVNGDAVAVQRSMQCTQKGKKTEFKTLEGVITRIKHGEKVSLSSKCAEIDREMISSLGVSRAVLNHVIFCHQEESNWPLSEGKALKQKFDEIFSATRYIKVLETLRTLRQKQTNTVKSCQMELKYLKQNKDKAQEIRELLSTKETQLASSKESVNRIEGQIDPLERRLNDIESSLGKVMKLDNDIKALDSRKKQMEDDNRELEEKMEQVFQGSDDQLQDMYQNHQRTVKEKEKRLVECQRELERAGRECQRMNRIKSELLVEQGRLQLEADRHTQNIKKRDTQVKTLASFLELEGYDRTPLSERQLQSFYRQIKERLDQDSEALNQTMHDMQQKETQKQHNIDDLRDKKTGLERTIELKKDLQAKKQQELKNIKSDLQKLEGSSNRLQELDTELQKAERELDNAVQACTVDSLKVEVTELLKEKAQLDQAQRKLDQEMEMLNTHTTARAQMDMMKKTKMDKEEQVRKIKSRHNEELVSLLGHFPNKKELEDWIYSKSREIKSTREQITKMNKELASGEQKKSHYTAEIKRKEEQLAKYEERLFNVCGSQDFQSDLSKLEDELEKCSKQRAMLAGATAVYSQFISQLTEEGDPCCPVCQRVFPSEAELQDVINDMQSKLRLVPDKLKNTEHDLKRKERRRDEMMTLKPIRQSLVELQEKELPELRNQLQRVNRDIEKLKGDIEEQETLLCTLVSEEETAKACLQDISLMDRFQLDLKDVERKIAQHAAKLQGVDLSRTMQQVSQEKQETQHRLDTTSSKIELKRKLIQDQQEQIQALRSSVNEIRGEKLQISSNMQKRQQLEEQCVEFSTEIQTLHRDIRDAKEQASPLAATLEKLQQEKQDLVERRRKKQEEGQEKINAIKEKMKNITLFEKEITKYIEEGKDSYKEQKETELQEMDKQLHEAEKQREKTNKDMGNIRQDIDTQKVQERWLQDNLTLRKRVEELKEVSRKREALIKEMGNMQVLQLRNERREVERKLEDLKKNRSVALGRQKGYEDEILRFRKELNEDQYCRAEDLYRDKMIVMRTTELANKDLDIYYKALDQTIMRFHSMKMEEINKIIRDLWRSTYRGQDIEYVEIRSDVDENASAGVKRRTYNYRVVMVKGDAALDMRGRCSAGQKVLASLIIRLALAETFCLNCGILALDEPTTNLDRENIESLAHALVEIIKSRSRQRNFQLLVITHDEDFVELLGRSNYVEHFYRIRKNQDQCSEISKCSINSLNSYLH

Target ORF information:

RefSeq Version	XM_005167938.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio RAD50 homolog, double strand break repair protein (rad50), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167938.4

ORF Insert Sequence:

ATGTCAAAGA TTGAGAAGAT GAGTATTCTG GGAGTGAGGA GTTTTGGAGT GGAGGATAAA 
GATAAACAAG TGATCTCCTT CTTCAGCCCT CTCACGGTGC TTGTGGGACC TAATGGAGCT 
GGCAAAACGA CCATCATTGA GTGTCTCAAG TACATCACAT CAGGAGACTT TCCACCAGGA 
AGTAAAGGAA ACACTTTTGT CCATGATCCC AAAGATGCTC ATGAGACTGA TGTACGGGCA 
CAGATCAGAC TCCAGTTTAG AGATGTGAAT GGAGATGCTG TGGCTGTGCA AAGATCTATG 
CAGTGCACAC AGAAAGGCAA GAAAACAGAA TTCAAAACGC TTGAGGGCGT CATTACGAGA 
ATAAAGCATG GTGAGAAAGT AAGCCTGAGC TCTAAATGTG CTGAGATAGA CCGTGAGATG 
ATCTCTTCCC TGGGCGTCTC CAGAGCTGTT CTCAACCATG TCATCTTTTG CCACCAAGAA 
GAGTCCAACT GGCCTCTCAG TGAAGGGAAA GCCCTCAAGC AGAAGTTTGA TGAGATTTTC 
TCAGCCACAA GGTACATTAA GGTGCTGGAG ACCTTGCGGA CGCTGAGGCA GAAGCAGACC 
AACACTGTTA AATCCTGCCA GATGGAGCTC AAGTACCTGA AACAGAACAA AGATAAAGCC 
CAAGAGATTC GAGAGCTGCT TTCCACCAAA GAAACTCAGC TAGCTTCCTC CAAAGAGAGT 
GTCAATCGCA TCGAGGGTCA GATTGACCCT CTGGAGAGAC GACTAAATGA CATAGAAAGC 
AGCCTCGGCA AAGTGATGAA ATTGGACAAC GACATCAAAG CTCTGGACAG TAGGAAGAAA 
CAGATGGAGG ATGATAACCG GGAGCTAGAG GAAAAGATGG AACAGGTGTT TCAGGGCTCA 
GATGATCAGC TACAGGACAT GTATCAGAAT CACCAGCGCA CCGTGAAAGA GAAGGAAAAA 
AGACTTGTAG AGTGCCAACG TGAGCTTGAA AGGGCTGGTC GAGAATGCCA GAGGATGAAC 
CGCATCAAGT CTGAGCTGCT AGTGGAGCAA GGTCGTCTGC AGTTGGAGGC AGATAGACAC 
ACACAGAACA TTAAAAAGAG AGACACCCAG GTAAAGACTT TGGCGTCTTT CCTGGAGTTG 
GAGGGCTATG ATCGAACGCC ACTTAGTGAG CGACAACTCC AAAGCTTTTA CAGACAAATC 
AAAGAAAGAC TGGACCAGGA CTCGGAAGCC CTCAACCAAA CTATGCATGA CATGCAGCAG 
AAAGAGACCC AGAAACAGCA CAACATTGAT GATCTCCGGG ATAAAAAGAC AGGTCTAGAG 
AGAACCATTG AGCTAAAGAA AGACCTACAG GCCAAAAAGC AACAAGAACT GAAGAATATC 
AAGTCTGACC TTCAAAAACT GGAGGGCTCC TCCAACAGAC TGCAAGAGCT GGACACTGAG 
CTTCAGAAAG CGGAGCGTGA GCTTGATAAT GCTGTGCAGG CCTGTACAGT GGACAGTCTG 
AAGGTCGAGG TAACTGAACT ACTGAAGGAA AAGGCTCAAC TGGATCAGGC ACAGCGCAAA 
CTGGACCAAG AGATGGAAAT GCTCAACACA CACACCACGG CACGTGCACA GATGGACATG 
ATGAAGAAAA CCAAGATGGA CAAAGAGGAG CAGGTGAGGA AGATCAAGTC GAGACATAAT 
GAAGAGCTGG TGTCTCTGCT GGGACACTTT CCAAACAAGA AAGAGCTGGA GGACTGGATC 
TACTCCAAAT CCAGAGAGAT CAAATCCACT AGAGAGCAGA TCACCAAAAT GAATAAAGAG 
CTTGCATCTG GAGAACAGAA GAAGAGTCAT TACACTGCAG AAATCAAACG AAAAGAAGAG 
CAGCTGGCCA AATATGAAGA GAGACTTTTT AATGTTTGTG GAAGCCAGGA TTTCCAGTCA 
GACTTAAGCA AACTGGAGGA TGAGTTGGAA AAGTGCTCCA AACAGAGAGC CATGTTGGCA 
GGTGCCACAG CTGTGTACAG TCAGTTCATC AGTCAGCTGA CGGAGGAAGG TGACCCATGC 
TGCCCCGTAT GTCAGCGCGT GTTCCCATCA GAGGCGGAGC TCCAGGACGT CATTAATGAC 
ATGCAGTCCA AACTGCGGCT TGTCCCAGAC AAGCTGAAGA ATACGGAGCA TGACTTGAAG 
AGGAAGGAGC GCAGACGTGA TGAAATGATG ACACTGAAGC CCATCAGGCA GTCCTTAGTG 
GAGCTCCAAG AGAAAGAGCT ACCAGAGTTA AGAAATCAAC TCCAGCGTGT GAATCGGGAC 
ATTGAGAAAC TGAAAGGAGA CATCGAGGAG CAGGAGACAT TGCTGTGCAC GCTGGTGTCT 
GAGGAGGAGA CAGCCAAAGC CTGTCTGCAG GACATCTCTC TCATGGATCG CTTTCAGCTT 
GATCTAAAGG ATGTAGAGAG GAAGATTGCT CAACATGCAG CCAAGTTGCA GGGAGTTGAC 
CTCAGTCGCA CCATGCAGCA GGTCAGCCAG GAGAAACAGG AGACTCAACA CCGCCTGGAC 
ACTACTTCCA GTAAGATCGA GCTAAAGAGG AAACTAATTC AGGACCAGCA GGAGCAGATC 
CAGGCCTTGA GGAGCAGTGT GAACGAGATT CGTGGAGAAA AGCTGCAGAT CTCCAGCAAC 
ATGCAGAAGC GTCAGCAGTT GGAGGAGCAG TGTGTGGAGT TCTCCACCGA GATCCAGACA 
CTCCACAGGG ACATTCGAGA TGCAAAGGAG CAGGCCTCTC CTCTAGCTGC TACTTTGGAG 
AAGCTCCAGC AGGAGAAACA GGACCTCGTT GAGCGCAGGA GGAAAAAACA GGAGGAAGGA 
CAGGAAAAGA TAAATGCGAT TAAGGAAAAA ATGAAAAACA TCACCCTCTT TGAAAAAGAG 
ATCACCAAGT ACATTGAAGA GGGCAAAGAC AGCTACAAAG AGCAAAAAGA AACTGAACTA 
CAGGAGATGG ACAAACAACT ACATGAGGCC GAAAAACAGC GGGAGAAGAC CAATAAAGAC 
ATGGGCAACA TCCGACAAGA CATTGACACT CAGAAGGTTC AAGAGCGCTG GCTGCAGGAC 
AACTTGACTC TACGAAAGCG CGTGGAGGAG CTGAAAGAGG TGTCCAGAAA ACGAGAGGCC 
TTAATTAAGG AAATGGGCAA CATGCAAGTC CTGCAGCTGC GCAATGAGCG ACGAGAAGTA 
GAGCGAAAGT TGGAGGACCT AAAAAAGAAC CGCAGTGTGG CGCTTGGTCG ACAGAAAGGC 
TACGAGGATG AGATCCTCCG CTTTCGTAAA GAACTCAATG AGGATCAATA TTGCCGGGCC 
GAGGATCTCT ACAGAGACAA AATGATTGTG ATGAGAACAA CAGAGCTGGC CAATAAAGAC 
CTGGACATCT ACTATAAAGC CCTAGACCAA ACAATAATGA GGTTTCACAG CATGAAGATG 
GAGGAGATTA ATAAAATCAT CAGAGATTTG TGGCGGAGCA CATACAGAGG ACAAGACATC 
GAGTATGTAG AGATTCGCTC TGATGTGGAT GAGAATGCAT CTGCTGGTGT GAAGAGAAGG 
ACCTATAACT ATAGAGTGGT AATGGTGAAA GGAGACGCAG CCTTGGACAT GCGGGGTCGA 
TGCAGTGCTG GACAAAAGGT TCTCGCCTCT CTTATAATCC GATTGGCTCT AGCTGAGACA 
TTCTGCCTGA ACTGTGGGAT TCTGGCACTG GATGAGCCCA CTACCAACTT AGACAGGGAG 
AATATAGAGT CTTTGGCACA TGCACTTGTG GAAATTATCA AAAGTCGCTC TCGTCAGCGC 
AACTTCCAGC TGCTTGTCAT CACTCATGAC GAGGATTTCG TGGAGCTCCT GGGAAGATCC 
AACTATGTGG AGCATTTCTA CAGGATCAGG AAGAACCAGG ATCAGTGCTC AGAGATCAGC 
AAATGCAGTA TCAACAGCCT CAACTCCTAC CTGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rad50 ( XM_691767.8 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_696859.4 Danio rerio RAD50 homolog, double strand break repair protein (rad50), transcript variant X2, mRNA.

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CloneID	ODa15615
Clone ID Related Accession (Same CDS sequence)	XM_691767.7 , XM_005167938.3 , XM_005167938.4 , XM_691767.8
Accession Version	XM_691767.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	DNA repair protein RAD50
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_691767.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGA TTGAGAAGAT GAGTATTCTG GGAGTGAGGA GTTTTGGAGT GGAGGATAAA 
GATAAACAAG TGATCTCCTT CTTCAGCCCT CTCACGGTGC TTGTGGGACC TAATGGAGCT 
GGCAAAACGA CCATCATTGA GTGTCTCAAG TACATCACAT CAGGAGACTT TCCACCAGGA 
AGTAAAGGAA ACACTTTTGT CCATGATCCC AAAGATGCTC ATGAGACTGA TGTACGGGCA 
CAGATCAGAC TCCAGTTTAG AGATGTGAAT GGAGATGCTG TGGCTGTGCA AAGATCTATG 
CAGTGCACAC AGAAAGGCAA GAAAACAGAA TTCAAAACGC TTGAGGGCGT CATTACGAGA 
ATAAAGCATG GTGAGAAAGT AAGCCTGAGC TCTAAATGTG CTGAGATAGA CCGTGAGATG 
ATCTCTTCCC TGGGCGTCTC CAGAGCTGTT CTCAACCATG TCATCTTTTG CCACCAAGAA 
GAGTCCAACT GGCCTCTCAG TGAAGGGAAA GCCCTCAAGC AGAAGTTTGA TGAGATTTTC 
TCAGCCACAA GGTACATTAA GGTGCTGGAG ACCTTGCGGA CGCTGAGGCA GAAGCAGACC 
AACACTGTTA AATCCTGCCA GATGGAGCTC AAGTACCTGA AACAGAACAA AGATAAAGCC 
CAAGAGATTC GAGAGCTGCT TTCCACCAAA GAAACTCAGC TAGCTTCCTC CAAAGAGAGT 
GTCAATCGCA TCGAGGGTCA GATTGACCCT CTGGAGAGAC GACTAAATGA CATAGAAAGC 
AGCCTCGGCA AAGTGATGAA ATTGGACAAC GACATCAAAG CTCTGGACAG TAGGAAGAAA 
CAGATGGAGG ATGATAACCG GGAGCTAGAG GAAAAGATGG AACAGGTGTT TCAGGGCTCA 
GATGATCAGC TACAGGACAT GTATCAGAAT CACCAGCGCA CCGTGAAAGA GAAGGAAAAA 
AGACTTGTAG AGTGCCAACG TGAGCTTGAA AGGGCTGGTC GAGAATGCCA GAGGATGAAC 
CGCATCAAGT CTGAGCTGCT AGTGGAGCAA GGTCGTCTGC AGTTGGAGGC AGATAGACAC 
ACACAGAACA TTAAAAAGAG AGACACCCAG GTAAAGACTT TGGCGTCTTT CCTGGAGTTG 
GAGGGCTATG ATCGAACGCC ACTTAGTGAG CGACAACTCC AAAGCTTTTA CAGACAAATC 
AAAGAAAGAC TGGACCAGGA CTCGGAAGCC CTCAACCAAA CTATGCATGA CATGCAGCAG 
AAAGAGACCC AGAAACAGCA CAACATTGAT GATCTCCGGG ATAAAAAGAC AGGTCTAGAG 
AGAACCATTG AGCTAAAGAA AGACCTACAG GCCAAAAAGC AACAAGAACT GAAGAATATC 
AAGTCTGACC TTCAAAAACT GGAGGGCTCC TCCAACAGAC TGCAAGAGCT GGACACTGAG 
CTTCAGAAAG CGGAGCGTGA GCTTGATAAT GCTGTGCAGG CCTGTACAGT GGACAGTCTG 
AAGGTCGAGG TAACTGAACT ACTGAAGGAA AAGGCTCAAC TGGATCAGGC ACAGCGCAAA 
CTGGACCAAG AGATGGAAAT GCTCAACACA CACACCACGG CACGTGCACA GATGGACATG 
ATGAAGAAAA CCAAGATGGA CAAAGAGGAG CAGGTGAGGA AGATCAAGTC GAGACATAAT 
GAAGAGCTGG TGTCTCTGCT GGGACACTTT CCAAACAAGA AAGAGCTGGA GGACTGGATC 
TACTCCAAAT CCAGAGAGAT CAAATCCACT AGAGAGCAGA TCACCAAAAT GAATAAAGAG 
CTTGCATCTG GAGAACAGAA GAAGAGTCAT TACACTGCAG AAATCAAACG AAAAGAAGAG 
CAGCTGGCCA AATATGAAGA GAGACTTTTT AATGTTTGTG GAAGCCAGGA TTTCCAGTCA 
GACTTAAGCA AACTGGAGGA TGAGTTGGAA AAGTGCTCCA AACAGAGAGC CATGTTGGCA 
GGTGCCACAG CTGTGTACAG TCAGTTCATC AGTCAGCTGA CGGAGGAAGG TGACCCATGC 
TGCCCCGTAT GTCAGCGCGT GTTCCCATCA GAGGCGGAGC TCCAGGACGT CATTAATGAC 
ATGCAGTCCA AACTGCGGCT TGTCCCAGAC AAGCTGAAGA ATACGGAGCA TGACTTGAAG 
AGGAAGGAGC GCAGACGTGA TGAAATGATG ACACTGAAGC CCATCAGGCA GTCCTTAGTG 
GAGCTCCAAG AGAAAGAGCT ACCAGAGTTA AGAAATCAAC TCCAGCGTGT GAATCGGGAC 
ATTGAGAAAC TGAAAGGAGA CATCGAGGAG CAGGAGACAT TGCTGTGCAC GCTGGTGTCT 
GAGGAGGAGA CAGCCAAAGC CTGTCTGCAG GACATCTCTC TCATGGATCG CTTTCAGCTT 
GATCTAAAGG ATGTAGAGAG GAAGATTGCT CAACATGCAG CCAAGTTGCA GGGAGTTGAC 
CTCAGTCGCA CCATGCAGCA GGTCAGCCAG GAGAAACAGG AGACTCAACA CCGCCTGGAC 
ACTACTTCCA GTAAGATCGA GCTAAAGAGG AAACTAATTC AGGACCAGCA GGAGCAGATC 
CAGGCCTTGA GGAGCAGTGT GAACGAGATT CGTGGAGAAA AGCTGCAGAT CTCCAGCAAC 
ATGCAGAAGC GTCAGCAGTT GGAGGAGCAG TGTGTGGAGT TCTCCACCGA GATCCAGACA 
CTCCACAGGG ACATTCGAGA TGCAAAGGAG CAGGCCTCTC CTCTAGCTGC TACTTTGGAG 
AAGCTCCAGC AGGAGAAACA GGACCTCGTT GAGCGCAGGA GGAAAAAACA GGAGGAAGGA 
CAGGAAAAGA TAAATGCGAT TAAGGAAAAA ATGAAAAACA TCACCCTCTT TGAAAAAGAG 
ATCACCAAGT ACATTGAAGA GGGCAAAGAC AGCTACAAAG AGCAAAAAGA AACTGAACTA 
CAGGAGATGG ACAAACAACT ACATGAGGCC GAAAAACAGC GGGAGAAGAC CAATAAAGAC 
ATGGGCAACA TCCGACAAGA CATTGACACT CAGAAGGTTC AAGAGCGCTG GCTGCAGGAC 
AACTTGACTC TACGAAAGCG CGTGGAGGAG CTGAAAGAGG TGTCCAGAAA ACGAGAGGCC 
TTAATTAAGG AAATGGGCAA CATGCAAGTC CTGCAGCTGC GCAATGAGCG ACGAGAAGTA 
GAGCGAAAGT TGGAGGACCT AAAAAAGAAC CGCAGTGTGG CGCTTGGTCG ACAGAAAGGC 
TACGAGGATG AGATCCTCCG CTTTCGTAAA GAACTCAATG AGGATCAATA TTGCCGGGCC 
GAGGATCTCT ACAGAGACAA AATGATTGTG ATGAGAACAA CAGAGCTGGC CAATAAAGAC 
CTGGACATCT ACTATAAAGC CCTAGACCAA ACAATAATGA GGTTTCACAG CATGAAGATG 
GAGGAGATTA ATAAAATCAT CAGAGATTTG TGGCGGAGCA CATACAGAGG ACAAGACATC 
GAGTATGTAG AGATTCGCTC TGATGTGGAT GAGAATGCAT CTGCTGGTGT GAAGAGAAGG 
ACCTATAACT ATAGAGTGGT AATGGTGAAA GGAGACGCAG CCTTGGACAT GCGGGGTCGA 
TGCAGTGCTG GACAAAAGGT TCTCGCCTCT CTTATAATCC GATTGGCTCT AGCTGAGACA 
TTCTGCCTGA ACTGTGGGAT TCTGGCACTG GATGAGCCCA CTACCAACTT AGACAGGGAG 
AATATAGAGT CTTTGGCACA TGCACTTGTG GAAATTATCA AAAGTCGCTC TCGTCAGCGC 
AACTTCCAGC TGCTTGTCAT CACTCATGAC GAGGATTTCG TGGAGCTCCT GGGAAGATCC 
AACTATGTGG AGCATTTCTA CAGGATCAGG AAGAACCAGG ATCAGTGCTC AGAGATCAGC 
AAATGCAGTA TCAACAGCCT CAACTCCTAC CTGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_696859.4
CDS	85..4023
Translation	MSKIEKMSILGVRSFGVEDKDKQVISFFSPLTVLVGPNGAGKTTIIECLKYITSGDFPPGSKGNTFVHDPKDAHETDVRAQIRLQFRDVNGDAVAVQRSMQCTQKGKKTEFKTLEGVITRIKHGEKVSLSSKCAEIDREMISSLGVSRAVLNHVIFCHQEESNWPLSEGKALKQKFDEIFSATRYIKVLETLRTLRQKQTNTVKSCQMELKYLKQNKDKAQEIRELLSTKETQLASSKESVNRIEGQIDPLERRLNDIESSLGKVMKLDNDIKALDSRKKQMEDDNRELEEKMEQVFQGSDDQLQDMYQNHQRTVKEKEKRLVECQRELERAGRECQRMNRIKSELLVEQGRLQLEADRHTQNIKKRDTQVKTLASFLELEGYDRTPLSERQLQSFYRQIKERLDQDSEALNQTMHDMQQKETQKQHNIDDLRDKKTGLERTIELKKDLQAKKQQELKNIKSDLQKLEGSSNRLQELDTELQKAERELDNAVQACTVDSLKVEVTELLKEKAQLDQAQRKLDQEMEMLNTHTTARAQMDMMKKTKMDKEEQVRKIKSRHNEELVSLLGHFPNKKELEDWIYSKSREIKSTREQITKMNKELASGEQKKSHYTAEIKRKEEQLAKYEERLFNVCGSQDFQSDLSKLEDELEKCSKQRAMLAGATAVYSQFISQLTEEGDPCCPVCQRVFPSEAELQDVINDMQSKLRLVPDKLKNTEHDLKRKERRRDEMMTLKPIRQSLVELQEKELPELRNQLQRVNRDIEKLKGDIEEQETLLCTLVSEEETAKACLQDISLMDRFQLDLKDVERKIAQHAAKLQGVDLSRTMQQVSQEKQETQHRLDTTSSKIELKRKLIQDQQEQIQALRSSVNEIRGEKLQISSNMQKRQQLEEQCVEFSTEIQTLHRDIRDAKEQASPLAATLEKLQQEKQDLVERRRKKQEEGQEKINAIKEKMKNITLFEKEITKYIEEGKDSYKEQKETELQEMDKQLHEAEKQREKTNKDMGNIRQDIDTQKVQERWLQDNLTLRKRVEELKEVSRKREALIKEMGNMQVLQLRNERREVERKLEDLKKNRSVALGRQKGYEDEILRFRKELNEDQYCRAEDLYRDKMIVMRTTELANKDLDIYYKALDQTIMRFHSMKMEEINKIIRDLWRSTYRGQDIEYVEIRSDVDENASAGVKRRTYNYRVVMVKGDAALDMRGRCSAGQKVLASLIIRLALAETFCLNCGILALDEPTTNLDRENIESLAHALVEIIKSRSRQRNFQLLVITHDEDFVELLGRSNYVEHFYRIRKNQDQCSEISKCSINSLNSYLH

Target ORF information:

RefSeq Version	XM_691767.8
Organism	Danio rerio(zebrafish)
Definition	Danio rerio RAD50 homolog, double strand break repair protein (rad50), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_691767.8

ORF Insert Sequence:

ATGTCAAAGA TTGAGAAGAT GAGTATTCTG GGAGTGAGGA GTTTTGGAGT GGAGGATAAA 
GATAAACAAG TGATCTCCTT CTTCAGCCCT CTCACGGTGC TTGTGGGACC TAATGGAGCT 
GGCAAAACGA CCATCATTGA GTGTCTCAAG TACATCACAT CAGGAGACTT TCCACCAGGA 
AGTAAAGGAA ACACTTTTGT CCATGATCCC AAAGATGCTC ATGAGACTGA TGTACGGGCA 
CAGATCAGAC TCCAGTTTAG AGATGTGAAT GGAGATGCTG TGGCTGTGCA AAGATCTATG 
CAGTGCACAC AGAAAGGCAA GAAAACAGAA TTCAAAACGC TTGAGGGCGT CATTACGAGA 
ATAAAGCATG GTGAGAAAGT AAGCCTGAGC TCTAAATGTG CTGAGATAGA CCGTGAGATG 
ATCTCTTCCC TGGGCGTCTC CAGAGCTGTT CTCAACCATG TCATCTTTTG CCACCAAGAA 
GAGTCCAACT GGCCTCTCAG TGAAGGGAAA GCCCTCAAGC AGAAGTTTGA TGAGATTTTC 
TCAGCCACAA GGTACATTAA GGTGCTGGAG ACCTTGCGGA CGCTGAGGCA GAAGCAGACC 
AACACTGTTA AATCCTGCCA GATGGAGCTC AAGTACCTGA AACAGAACAA AGATAAAGCC 
CAAGAGATTC GAGAGCTGCT TTCCACCAAA GAAACTCAGC TAGCTTCCTC CAAAGAGAGT 
GTCAATCGCA TCGAGGGTCA GATTGACCCT CTGGAGAGAC GACTAAATGA CATAGAAAGC 
AGCCTCGGCA AAGTGATGAA ATTGGACAAC GACATCAAAG CTCTGGACAG TAGGAAGAAA 
CAGATGGAGG ATGATAACCG GGAGCTAGAG GAAAAGATGG AACAGGTGTT TCAGGGCTCA 
GATGATCAGC TACAGGACAT GTATCAGAAT CACCAGCGCA CCGTGAAAGA GAAGGAAAAA 
AGACTTGTAG AGTGCCAACG TGAGCTTGAA AGGGCTGGTC GAGAATGCCA GAGGATGAAC 
CGCATCAAGT CTGAGCTGCT AGTGGAGCAA GGTCGTCTGC AGTTGGAGGC AGATAGACAC 
ACACAGAACA TTAAAAAGAG AGACACCCAG GTAAAGACTT TGGCGTCTTT CCTGGAGTTG 
GAGGGCTATG ATCGAACGCC ACTTAGTGAG CGACAACTCC AAAGCTTTTA CAGACAAATC 
AAAGAAAGAC TGGACCAGGA CTCGGAAGCC CTCAACCAAA CTATGCATGA CATGCAGCAG 
AAAGAGACCC AGAAACAGCA CAACATTGAT GATCTCCGGG ATAAAAAGAC AGGTCTAGAG 
AGAACCATTG AGCTAAAGAA AGACCTACAG GCCAAAAAGC AACAAGAACT GAAGAATATC 
AAGTCTGACC TTCAAAAACT GGAGGGCTCC TCCAACAGAC TGCAAGAGCT GGACACTGAG 
CTTCAGAAAG CGGAGCGTGA GCTTGATAAT GCTGTGCAGG CCTGTACAGT GGACAGTCTG 
AAGGTCGAGG TAACTGAACT ACTGAAGGAA AAGGCTCAAC TGGATCAGGC ACAGCGCAAA 
CTGGACCAAG AGATGGAAAT GCTCAACACA CACACCACGG CACGTGCACA GATGGACATG 
ATGAAGAAAA CCAAGATGGA CAAAGAGGAG CAGGTGAGGA AGATCAAGTC GAGACATAAT 
GAAGAGCTGG TGTCTCTGCT GGGACACTTT CCAAACAAGA AAGAGCTGGA GGACTGGATC 
TACTCCAAAT CCAGAGAGAT CAAATCCACT AGAGAGCAGA TCACCAAAAT GAATAAAGAG 
CTTGCATCTG GAGAACAGAA GAAGAGTCAT TACACTGCAG AAATCAAACG AAAAGAAGAG 
CAGCTGGCCA AATATGAAGA GAGACTTTTT AATGTTTGTG GAAGCCAGGA TTTCCAGTCA 
GACTTAAGCA AACTGGAGGA TGAGTTGGAA AAGTGCTCCA AACAGAGAGC CATGTTGGCA 
GGTGCCACAG CTGTGTACAG TCAGTTCATC AGTCAGCTGA CGGAGGAAGG TGACCCATGC 
TGCCCCGTAT GTCAGCGCGT GTTCCCATCA GAGGCGGAGC TCCAGGACGT CATTAATGAC 
ATGCAGTCCA AACTGCGGCT TGTCCCAGAC AAGCTGAAGA ATACGGAGCA TGACTTGAAG 
AGGAAGGAGC GCAGACGTGA TGAAATGATG ACACTGAAGC CCATCAGGCA GTCCTTAGTG 
GAGCTCCAAG AGAAAGAGCT ACCAGAGTTA AGAAATCAAC TCCAGCGTGT GAATCGGGAC 
ATTGAGAAAC TGAAAGGAGA CATCGAGGAG CAGGAGACAT TGCTGTGCAC GCTGGTGTCT 
GAGGAGGAGA CAGCCAAAGC CTGTCTGCAG GACATCTCTC TCATGGATCG CTTTCAGCTT 
GATCTAAAGG ATGTAGAGAG GAAGATTGCT CAACATGCAG CCAAGTTGCA GGGAGTTGAC 
CTCAGTCGCA CCATGCAGCA GGTCAGCCAG GAGAAACAGG AGACTCAACA CCGCCTGGAC 
ACTACTTCCA GTAAGATCGA GCTAAAGAGG AAACTAATTC AGGACCAGCA GGAGCAGATC 
CAGGCCTTGA GGAGCAGTGT GAACGAGATT CGTGGAGAAA AGCTGCAGAT CTCCAGCAAC 
ATGCAGAAGC GTCAGCAGTT GGAGGAGCAG TGTGTGGAGT TCTCCACCGA GATCCAGACA 
CTCCACAGGG ACATTCGAGA TGCAAAGGAG CAGGCCTCTC CTCTAGCTGC TACTTTGGAG 
AAGCTCCAGC AGGAGAAACA GGACCTCGTT GAGCGCAGGA GGAAAAAACA GGAGGAAGGA 
CAGGAAAAGA TAAATGCGAT TAAGGAAAAA ATGAAAAACA TCACCCTCTT TGAAAAAGAG 
ATCACCAAGT ACATTGAAGA GGGCAAAGAC AGCTACAAAG AGCAAAAAGA AACTGAACTA 
CAGGAGATGG ACAAACAACT ACATGAGGCC GAAAAACAGC GGGAGAAGAC CAATAAAGAC 
ATGGGCAACA TCCGACAAGA CATTGACACT CAGAAGGTTC AAGAGCGCTG GCTGCAGGAC 
AACTTGACTC TACGAAAGCG CGTGGAGGAG CTGAAAGAGG TGTCCAGAAA ACGAGAGGCC 
TTAATTAAGG AAATGGGCAA CATGCAAGTC CTGCAGCTGC GCAATGAGCG ACGAGAAGTA 
GAGCGAAAGT TGGAGGACCT AAAAAAGAAC CGCAGTGTGG CGCTTGGTCG ACAGAAAGGC 
TACGAGGATG AGATCCTCCG CTTTCGTAAA GAACTCAATG AGGATCAATA TTGCCGGGCC 
GAGGATCTCT ACAGAGACAA AATGATTGTG ATGAGAACAA CAGAGCTGGC CAATAAAGAC 
CTGGACATCT ACTATAAAGC CCTAGACCAA ACAATAATGA GGTTTCACAG CATGAAGATG 
GAGGAGATTA ATAAAATCAT CAGAGATTTG TGGCGGAGCA CATACAGAGG ACAAGACATC 
GAGTATGTAG AGATTCGCTC TGATGTGGAT GAGAATGCAT CTGCTGGTGT GAAGAGAAGG 
ACCTATAACT ATAGAGTGGT AATGGTGAAA GGAGACGCAG CCTTGGACAT GCGGGGTCGA 
TGCAGTGCTG GACAAAAGGT TCTCGCCTCT CTTATAATCC GATTGGCTCT AGCTGAGACA 
TTCTGCCTGA ACTGTGGGAT TCTGGCACTG GATGAGCCCA CTACCAACTT AGACAGGGAG 
AATATAGAGT CTTTGGCACA TGCACTTGTG GAAATTATCA AAAGTCGCTC TCGTCAGCGC 
AACTTCCAGC TGCTTGTCAT CACTCATGAC GAGGATTTCG TGGAGCTCCT GGGAAGATCC 
AACTATGTGG AGCATTTCTA CAGGATCAGG AAGAACCAGG ATCAGTGCTC AGAGATCAGC 
AAATGCAGTA TCAACAGCCT CAACTCCTAC CTGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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rad50 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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