pik3c3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	pik3c3
Entrez Gene ID	548346
Full Name	phosphatidylinositol 3-kinase, catalytic subunit type 3
Synonyms	im:7147158,wu:fc13g06,wu:fz51e12,zgc:112016,zgc:113009
General protein information	Preferred Names phosphatidylinositol 3-kinase, catalytic subunit type 3 Names phosphatidylinositol 3-kinase catalytic subunit type 3 phosphoinositide-3-kinase, class 3
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001328533.1	NP_001315462.1	phosphatidylinositol 3-kinase catalytic subunit type 3
XM_021477525.1	XP_021333200.1	phosphatidylinositol 3-kinase catalytic subunit type 3 isoform X1

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0D18645g	XP_453887.1
Caenorhabditis elegans (roundworm)	vps-34	NP_001020954.1
Saccharomyces cerevisiae (baker's yeast)	VPS34	NP_013341.1
Arabidopsis thaliana (thale cress)	VPS34	NP_176251.1
Eremothecium gossypii	AGOS_AGL113C	NP_986554.1
Gallus gallus (chicken)	PIK3C3	XP_003642950.2
Pan troglodytes (chimpanzee)	PIK3C3	XP_001142812.1
Mus musculus (house mouse)	Pik3c3	NP_852079.2
Drosophila melanogaster (fruit fly)	Pi3K59F	NP_477133.1
Schizosaccharomyces pombe (fission yeast)	pik3	NP_594699.1
Xenopus tropicalis (tropical clawed frog)	pik3c3	XP_002935097.2
Canis lupus familiaris (dog)	PIK3C3	XP_547599.3
Homo sapiens (human)	PIK3C3	NP_002638.2
Oryza sativa (rice)	Os05g0180600	NP_001054810.1
Magnaporthe oryzae (rice blast fungus)	MGG_03069	XP_003720606.1
Macaca mulatta (Rhesus monkey)	PIK3C3	XP_001082892.1
Bos taurus (cattle)	PIK3C3	NP_001092537.1
Rattus norvegicus (Norway rat)	Pik3c3	NP_075247.1
Danio rerio (zebrafish)	pik3c3	NP_001017550.2
Neurospora crassa	NCU00656	XP_965796.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010273	XP_319464.4

Gene Ontology
Bibliography

Related articles in PubMed

Deficiency in class III PI3-kinase confers postnatal lethality with IBD-like features in zebrafish.
Zhao S, Xia J, Wu X, Zhang L, Wang P, Wang H, Li H, Wang X, Chen Y, Agnetti J, Li Y, Pei D, Shu X
Nature communications9(1)2639(2018 07)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

The zebrafish gene map defines ancestral vertebrate chromosomes.
Woods IG, Wilson C, Friedlander B, Chang P, Reyes DK, Nix R, Kelly PD, Chu F, Postlethwait JH, Talbot WS
Genome research15(9)1307-14(2005 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following pik3c3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pik3c3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa12902	NM_001328533.1 Latest version!	Danio rerio phosphatidylinositol 3-kinase, catalytic subunit type 3 (pik3c3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa143289	XM_021477525.1 Latest version!	Danio rerio phosphatidylinositol 3-kinase, catalytic subunit type 3 (pik3c3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pik3c3 ( NM_001328533.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001315462.1 Danio rerio phosphatidylinositol 3-kinase, catalytic subunit type 3 (pik3c3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa12902
Clone ID Related Accession (Same CDS sequence)	NM_001328533.1
Accession Version	NM_001328533.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2637bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1545408000000
Organism	Danio rerio(zebrafish)
Product	phosphatidylinositol 3-kinase catalytic subunit type 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX510349.5, EH542626.1 and EH563762.1. On Jun 17, 2016 this sequence version replaced XM_005172281.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01030375.1, GDQH01001791.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGACACTG ACAAGTTTAA CTATGTGTAC AGTTGCGATT TGGACATAAA CGTTCAACTG 
AAAATAGGCA GTCTGGAGGG AAAGCGAGAG CAGAAGAGCT ACAAAGCTCT GCTTGAAGAC 
CCCATGCTGC GATTCTCAGG ACTCTATCAG GAGAGCTGCT CTGATCTGTA CGTCACCTGT 
CAGGTGTTTG CTGAGGGAAA ACCACTTGCC CTGCCTGTAC GAACCTCTTA TAAGGCCTTC 
AGCACTCGCT GGAACTGGAA TGAATGGCTC CGGCTGCCCG TGAAGTATCC TGACCTGCCT 
CAGAGTGCCC AGGTGACTCT GACCGTGTGG GACGTGTATG GACCTGGCCG CGCCACACCT 
GTTGGGGGCA CTACAGTCAC CTTGTTTGGA AAATATGGAA TGTTCCGGCA GGGCATGCAT 
GATCTGAAGG TGTGGCCGGG TGTGGAGGGA GATGGAACTG AGCCCACCAG TACACCAGGA 
CGGACCAGCA GCTCTCTGGC AGAAGACCAG ATGGGCCGCC TGGCCAAGCT AACAAAAGCT 
CACCGTCAGG GACACATGGT TAAAGTGGAC TGGCTGGATC GCTTGACCTT CCGAGAGATT 
GAGATGATCA ATGAGAGTGA GAAGCGTAGC TCTAATTTTA TGTACCTTAT GGTTGAATTC 
CCTCGTGTCA AAAGTGGTGA ACGTGAATAC AGCATTGTCT ATTACGAAAA GGATGCAGAT 
GAATCCTCTC CTTTGCCGAC CAGTTCAGAT ATTGTGAAAG TGCCTGACCC CCAGATGTGC 
ATGGAAAATC TAGTGGAGAG TAAGCATCAT AAACTGGCCC GTAGTTTGCG CAGTGGACCT 
TCTGACCACG ATCTCAAACC TAACGCAGCC ACACGCGATC AACTTAATAT CATAGTGAGC 
TACCCACCTA CAAAACAGCT GAGCTCTGAG GAACAGGACC TTGTGTGGAA GTTTAGATAC 
TACCTCACCA CACAGGAGAA GGCTCTCACT AAGTTCCTGA AGTGTGTGAA CTGGGATCTG 
CCTCAGGAGG CCAAACAGGC GCTGGAGCTA CTGGGAAAGT GGCGTCCGAT GGATGTGGAG 
GATTCACTGG AGCTGCTGTC GTCGCAGTTC ACCAACCCCA CCGTCAGACG CTATGCCGTC 
GCCAGGCTAC AGCAGGCCGA TGATGAGGAT CTGCTGATGT ACTTGCTCCA GTTGGTGCAG 
GCATTGAAAT ATGAGAATTT CAACGATATC CAGGGTGGTT TGGAGCCTGC CAATAAAAGA 
GACAATCAGG GGGGTTTGAC CGAGAGCTCG ACAACAGGAG ATCTGGACAG TTCTCAGTTG 
GCATCAGCTA TTGCAGTGAT GCCCAGCTCA CAAAAAGGCA AAGAGGGAAC AGATGGTGAA 
AATCTAGAGC AAGATCTGTG CACCTTCCTT ATATCACGTG CCTGCAAAAA CTCTACACTG 
GCAAACTATT TATACTGGTA TGTGATAGTG GAGTGTGAGG ATCAGGACAC GCAGCAGAGA 
GACCCAAAAA CCCATGACAT GTACCTCAAT GTGATGAGGC GCTTCAGTCA GGCTCTTCTT 
AAGGGTGATA AGAGTGTGCG AGTGATGCGT TCACTTCTGG CCTCCCAGCA GACGTTTGTA 
GATAGACTGG TGCAGCTCAT GAAAGCTGTT CAAAGAGAGA GCGGAAACCG CAAGAAAAAG 
ACGGAGCGCT TACAGAGCCT GTTGGCTGAT AATGAGAAGG TGAATCTGTC AGAGATTGAG 
CCCATTCCTC TCCCTCTGGA GCCACAGATC CGCATCAAAG GCATCATTCC TGAGACCGCA 
ACGCTCTTCA AGAGTGCTCT GATGCCAGCA AAGCTCATCT TTAAGACTGA AGATGGAGAG 
CAATATCCTG TCATCTTCAA ACATGGGGAT GACCTGCGGC AGGACCAGCT CATCCTGCAG 
ATTATCTCAC TCATGGACAA GTTGCTGAGG AAAGAGAATT TGGACCTGAA GTTGACCCCA 
TACAAAGTTT TGGCGACCAG CACCAAACAC GGTTTTATGC AGTTTGTGCA GTCAGTGCCT 
GTGGCTGAGG TTTTAGCGAC TGAAGGAAAC ATTCAGAGTT TCTTCAGAAA GTACGCACCC 
AATGATAAAG GACCGTATGG CATCAGCTCT GAGGTCATGG ACACGTATGT TAAAAGCTGT 
GCGGGTTATT GTGTGATCAC CTATATCCTC GGGGTCGGAG ACCGACATCT AGACAACCTG 
CTTCTCACCA AGACTGGGAA GCTGTTTCAT ATAGATTTTG GGTATATTTT GGGTCGTGAC 
CCTAAACCCC TGCCGCCCCC TATGAAGCTG AGCAAGGAAA TGGTGGAGGG GATGGGGGGC 
ATGCAGAGCG AGCAATACCA GGAATTCAGG AAACAGTGCT ACACCGCCTT CCTGCACCTA 
CGCAGATACT CCAATCTCAT TTTGAATCTG TTCTCTCTGA TGGTGGATGC TAATATTCCA 
GACATTGCGC TGGAGCCTGA CAAAACCGTA AAAAAGGTTC AGGACAAGTT TCGATTGGAC 
CTCTCGGATG AGGAGGCGGT GCATTACATG CAGAGTCTGA TTGATGAGAG CGTTGGAGCA 
TTGTTTGCTG CTGTGGTTGA ACAGATACAC AAGTTTGCAC AGTATTGGCG CAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001315462.1
CDS	313..2949
Translation	MDTDKFNYVYSCDLDINVQLKIGSLEGKREQKSYKALLEDPMLRFSGLYQESCSDLYVTCQVFAEGKPLALPVRTSYKAFSTRWNWNEWLRLPVKYPDLPQSAQVTLTVWDVYGPGRATPVGGTTVTLFGKYGMFRQGMHDLKVWPGVEGDGTEPTSTPGRTSSSLAEDQMGRLAKLTKAHRQGHMVKVDWLDRLTFREIEMINESEKRSSNFMYLMVEFPRVKSGEREYSIVYYEKDADESSPLPTSSDIVKVPDPQMCMENLVESKHHKLARSLRSGPSDHDLKPNAATRDQLNIIVSYPPTKQLSSEEQDLVWKFRYYLTTQEKALTKFLKCVNWDLPQEAKQALELLGKWRPMDVEDSLELLSSQFTNPTVRRYAVARLQQADDEDLLMYLLQLVQALKYENFNDIQGGLEPANKRDNQGGLTESSTTGDLDSSQLASAIAVMPSSQKGKEGTDGENLEQDLCTFLISRACKNSTLANYLYWYVIVECEDQDTQQRDPKTHDMYLNVMRRFSQALLKGDKSVRVMRSLLASQQTFVDRLVQLMKAVQRESGNRKKKTERLQSLLADNEKVNLSEIEPIPLPLEPQIRIKGIIPETATLFKSALMPAKLIFKTEDGEQYPVIFKHGDDLRQDQLILQIISLMDKLLRKENLDLKLTPYKVLATSTKHGFMQFVQSVPVAEVLATEGNIQSFFRKYAPNDKGPYGISSEVMDTYVKSCAGYCVITYILGVGDRHLDNLLLTKTGKLFHIDFGYILGRDPKPLPPPMKLSKEMVEGMGGMQSEQYQEFRKQCYTAFLHLRRYSNLILNLFSLMVDANIPDIALEPDKTVKKVQDKFRLDLSDEEAVHYMQSLIDESVGALFAAVVEQIHKFAQYWRR

Target ORF information:

RefSeq Version	NM_001328533.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphatidylinositol 3-kinase, catalytic subunit type 3 (pik3c3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001328533.1

ORF Insert Sequence:

ATGGACACTG ACAAGTTTAA CTATGTGTAC AGTTGCGATT TGGACATAAA CGTTCAACTG 
AAAATAGGCA GTCTGGAGGG AAAGCGAGAG CAGAAGAGCT ACAAAGCTCT GCTTGAAGAC 
CCCATGCTGC GATTCTCAGG ACTCTATCAG GAGAGCTGCT CTGATCTGTA CGTCACCTGT 
CAGGTGTTTG CTGAGGGAAA ACCACTTGCC CTGCCTGTAC GAACCTCTTA TAAGGCCTTC 
AGCACTCGCT GGAACTGGAA TGAATGGCTC CGGCTGCCCG TGAAGTATCC TGACCTGCCT 
CAGAGTGCCC AGGTGACTCT GACCGTGTGG GACGTGTATG GACCTGGCCG CGCCACACCT 
GTTGGGGGCA CTACAGTCAC CTTGTTTGGA AAATATGGAA TGTTCCGGCA GGGCATGCAT 
GATCTGAAGG TGTGGCCGGG TGTGGAGGGA GATGGAACTG AGCCCACCAG TACACCAGGA 
CGGACCAGCA GCTCTCTGGC AGAAGACCAG ATGGGCCGCC TGGCCAAGCT AACAAAAGCT 
CACCGTCAGG GACACATGGT TAAAGTGGAC TGGCTGGATC GCTTGACCTT CCGAGAGATT 
GAGATGATCA ATGAGAGTGA GAAGCGTAGC TCTAATTTTA TGTACCTTAT GGTTGAATTC 
CCTCGTGTCA AAAGTGGTGA ACGTGAATAC AGCATTGTCT ATTACGAAAA GGATGCAGAT 
GAATCCTCTC CTTTGCCGAC CAGTTCAGAT ATTGTGAAAG TGCCTGACCC CCAGATGTGC 
ATGGAAAATC TAGTGGAGAG TAAGCATCAT AAACTGGCCC GTAGTTTGCG CAGTGGACCT 
TCTGACCACG ATCTCAAACC TAACGCAGCC ACACGCGATC AACTTAATAT CATAGTGAGC 
TACCCACCTA CAAAACAGCT GAGCTCTGAG GAACAGGACC TTGTGTGGAA GTTTAGATAC 
TACCTCACCA CACAGGAGAA GGCTCTCACT AAGTTCCTGA AGTGTGTGAA CTGGGATCTG 
CCTCAGGAGG CCAAACAGGC GCTGGAGCTA CTGGGAAAGT GGCGTCCGAT GGATGTGGAG 
GATTCACTGG AGCTGCTGTC GTCGCAGTTC ACCAACCCCA CCGTCAGACG CTATGCCGTC 
GCCAGGCTAC AGCAGGCCGA TGATGAGGAT CTGCTGATGT ACTTGCTCCA GTTGGTGCAG 
GCATTGAAAT ATGAGAATTT CAACGATATC CAGGGTGGTT TGGAGCCTGC CAATAAAAGA 
GACAATCAGG GGGGTTTGAC CGAGAGCTCG ACAACAGGAG ATCTGGACAG TTCTCAGTTG 
GCATCAGCTA TTGCAGTGAT GCCCAGCTCA CAAAAAGGCA AAGAGGGAAC AGATGGTGAA 
AATCTAGAGC AAGATCTGTG CACCTTCCTT ATATCACGTG CCTGCAAAAA CTCTACACTG 
GCAAACTATT TATACTGGTA TGTGATAGTG GAGTGTGAGG ATCAGGACAC GCAGCAGAGA 
GACCCAAAAA CCCATGACAT GTACCTCAAT GTGATGAGGC GCTTCAGTCA GGCTCTTCTT 
AAGGGTGATA AGAGTGTGCG AGTGATGCGT TCACTTCTGG CCTCCCAGCA GACGTTTGTA 
GATAGACTGG TGCAGCTCAT GAAAGCTGTT CAAAGAGAGA GCGGAAACCG CAAGAAAAAG 
ACGGAGCGCT TACAGAGCCT GTTGGCTGAT AATGAGAAGG TGAATCTGTC AGAGATTGAG 
CCCATTCCTC TCCCTCTGGA GCCACAGATC CGCATCAAAG GCATCATTCC TGAGACCGCA 
ACGCTCTTCA AGAGTGCTCT GATGCCAGCA AAGCTCATCT TTAAGACTGA AGATGGAGAG 
CAATATCCTG TCATCTTCAA ACATGGGGAT GACCTGCGGC AGGACCAGCT CATCCTGCAG 
ATTATCTCAC TCATGGACAA GTTGCTGAGG AAAGAGAATT TGGACCTGAA GTTGACCCCA 
TACAAAGTTT TGGCGACCAG CACCAAACAC GGTTTTATGC AGTTTGTGCA GTCAGTGCCT 
GTGGCTGAGG TTTTAGCGAC TGAAGGAAAC ATTCAGAGTT TCTTCAGAAA GTACGCACCC 
AATGATAAAG GACCGTATGG CATCAGCTCT GAGGTCATGG ACACGTATGT TAAAAGCTGT 
GCGGGTTATT GTGTGATCAC CTATATCCTC GGGGTCGGAG ACCGACATCT AGACAACCTG 
CTTCTCACCA AGACTGGGAA GCTGTTTCAT ATAGATTTTG GGTATATTTT GGGTCGTGAC 
CCTAAACCCC TGCCGCCCCC TATGAAGCTG AGCAAGGAAA TGGTGGAGGG GATGGGGGGC 
ATGCAGAGCG AGCAATACCA GGAATTCAGG AAACAGTGCT ACACCGCCTT CCTGCACCTA 
CGCAGATACT CCAATCTCAT TTTGAATCTG TTCTCTCTGA TGGTGGATGC TAATATTCCA 
GACATTGCGC TGGAGCCTGA CAAAACCGTA AAAAAGGTTC AGGACAAGTT TCGATTGGAC 
CTCTCGGATG AGGAGGCGGT GCATTACATG CAGAGTCTGA TTGATGAGAG CGTTGGAGCA 
TTGTTTGCTG CTGTGGTTGA ACAGATACAC AAGTTTGCAC AGTATTGGCG CAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pik3c3 ( XM_021477525.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333200.1 Danio rerio phosphatidylinositol 3-kinase, catalytic subunit type 3 (pik3c3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa143289
Clone ID Related Accession (Same CDS sequence)	XM_021477525.1
Accession Version	XM_021477525.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	phosphatidylinositol 3-kinase catalytic subunit type 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTG ACAAGTTTAA CTATGTGTAC AGTTGCGATT TGGACATAAA CGTTCAACTG 
AAAATAGGCA GTCTGGAGGG AAAGCGAGAG CAGAAGAGCT ACAAAGCTCT GCTTGAAGAC 
CCCATGCTGC GATTCTCAGG ACTCTATCAG GAGAGCTGCT CTGATCTGTA CGTCACCTGT 
CAGGTGTTTG CTGAGGGAAA ACCACTTGCC CTGCCTGTAC GAACCTCTTA TAAGGCCTTC 
AGCACTCGCT GGAACTGGAA TGAATGGCTC CGGCTGCCCG TGAAGTATCC TGACCTGCCT 
CAGAGTGCCC AGGTGACTCT GACCGTGTGG GACGTGTATG GACCTGGCCG CGCCACACCT 
GTTGGGGGCA CTACAGTCAC CTTGTTTGGA AAATATGGAA TGTTCCGGCA GGGCATGCAT 
GATCTGAAGG TGTGGCCGGG TGTGGAGGGA GATGGAACTG AGCCCACCAG TACACCAGGA 
CGGACCAGCA GCTCTCTGGC AGAAGACCAG ATGGGCCGCC TGGCCAAGGG CCCAGACCTG 
GAGATACTTA GTGACCTAAC AAAAGCTCAC CGTCAGGGAC ACATGGTTAA AGTGGACTGG 
CTGGATCGCT TGACCTTCCG AGAGATTGAG ATGATCAATG AGAGTGAGAA GCGTAGCTCT 
AATTTTATGT ACCTTATGGT TGAATTCCCT CGTGTCAAAA GTGGTGAACG TGAATACAGC 
ATTGTCTATT ACGAAAAGGA TGCAGATGAA TCCTCTCCTT TGCCGACCAG TTCAGATATT 
GTGAAAGTGC CTGACCCCCA GATGTGCATG GAAAATCTAG TGGAGAGTAA GCATCATAAA 
CTGGCCCGTA GTTTGCGCAG TGGACCTTCT GACCACGATC TCAAACCTAA CGCAGCCACA 
CGCGATCAAC TTAATATCAT AGTGAGCTAC CCACCTACAA AACAGCTGAG CTCTGAGGAA 
CAGGACCTTG TGTGGAAGTT TAGATACTAC CTCACCACAC AGGAGAAGGC TCTCACTAAG 
TTCCTGAAGT GTGTGAACTG GGATCTGCCT CAGGAGGCCA AACAGGCGCT GGAGCTACTG 
GGAAAGTGGC GTCCGATGGA TGTGGAGGAT TCACTGGAGC TGCTGTCGTC GCAGTTCACC 
AACCCCACCG TCAGACGCTA TGCCGTCGCC AGGCTACAGC AGGCCGATGA TGAGGATCTG 
CTGATGTACT TGCTCCAGTT GGTGCAGGCA TTGAAATATG AGAATTTCAA CGATATCCAG 
GGTGGTTTGG AGCCTGCCAA TAAAAGAGAC AATCAGGGGG GTTTGACCGA GAGCTCGACA 
ACAGGAGATC TGGACAGTTC TCAGTTGGCA TCAGCTATTG CAGTGATGCC CAGCTCACAA 
AAAGGCAAAG AGGGAACAGA TGGTGAAAAT CTAGAGCAAG ATCTGTGCAC CTTCCTTATA 
TCACGTGCCT GCAAAAACTC TACACTGGCA AACTATTTAT ACTGGTATGT GATAGTGGAG 
TGTGAGGATC AGGACACGCA GCAGAGAGAC CCAAAAACCC ATGACATGTA CCTCAATGTG 
ATGAGGCGCT TCAGTCAGGC TCTTCTTAAG GGTGATAAGA GTGTGCGAGT GATGCGTTCA 
CTTCTGGCCT CCCAGCAGAC GTTTGTAGAT AGACTGGTGC AGCTCATGAA AGCTGTTCAA 
AGAGAGAGCG GAAACCGCAA GAAAAAGACG GAGCGCTTAC AGAGCCTGTT GGCTGATAAT 
GAGAAGGTGA ATCTGTCAGA GATTGAGCCC ATTCCTCTCC CTCTGGAGCC ACAGATCCGC 
ATCAAAGGCA TCATTCCTGA GACCGCAACG CTCTTCAAGA GTGCTCTGAT GCCAGCAAAG 
CTCATCTTTA AGACTGAAGA TGGAGAGCAA TATCCTGTCA TCTTCAAACA TGGGGATGAC 
CTGCGGCAGG ACCAGCTCAT CCTGCAGATT ATCTCACTCA TGGACAAGTT GCTGAGGAAA 
GAGAATTTGG ACCTGAAGTT GACCCCATAC AAAGTTTTGG CGACCAGCAC CAAACACGGT 
TTTATGCAGT TTGTGCAGTC AGTGCCTGTG GCTGAGGTTT TAGCGACTGA AGGAAACATT 
CAGAGTTTCT TCAGAAAGTA CGCACCCAAT GATAAAGGAC CGTATGGCAT CAGCTCTGAG 
GTCATGGACA CGTATGTTAA AAGCTGTGCG GGTTATTGTG TGATCACCTA TATCCTCGGG 
GTCGGAGACC GACATCTAGA CAACCTGCTT CTCACCAAGA CTGGGAAGCT GTTTCATATA 
GATTTTGGGT ATATTTTGGG TCGTGACCCT AAACCCCTGC CGCCCCCTAT GAAGCTGAGC 
AAGGAAATGG TGGAGGGGAT GGGGGGCATG CAGAGCGAGC AATACCAGGA ATTCAGGAAA 
CAGTGCTACA CCGCCTTCCT GCACCTACGC AGATACTCCA ATCTCATTTT GAATCTGTTC 
TCTCTGATGG TGGATGCTAA TATTCCAGAC ATTGCGCTGG AGCCTGACAA AACCGTAAAA 
AAGGTTCAGG ACAAGTTTCG ATTGGACCTC TCGGATGAGG AGGCGGTGCA TTACATGCAG 
AGTCTGATTG ATGAGAGCGT TGGAGCATTG TTTGCTGCTG TGGTTGAACA GATACACAAG 
TTTGCACAGT ATTGGCGCAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333200.1
CDS	345..3008
Translation	MDTDKFNYVYSCDLDINVQLKIGSLEGKREQKSYKALLEDPMLRFSGLYQESCSDLYVTCQVFAEGKPLALPVRTSYKAFSTRWNWNEWLRLPVKYPDLPQSAQVTLTVWDVYGPGRATPVGGTTVTLFGKYGMFRQGMHDLKVWPGVEGDGTEPTSTPGRTSSSLAEDQMGRLAKGPDLEILSDLTKAHRQGHMVKVDWLDRLTFREIEMINESEKRSSNFMYLMVEFPRVKSGEREYSIVYYEKDADESSPLPTSSDIVKVPDPQMCMENLVESKHHKLARSLRSGPSDHDLKPNAATRDQLNIIVSYPPTKQLSSEEQDLVWKFRYYLTTQEKALTKFLKCVNWDLPQEAKQALELLGKWRPMDVEDSLELLSSQFTNPTVRRYAVARLQQADDEDLLMYLLQLVQALKYENFNDIQGGLEPANKRDNQGGLTESSTTGDLDSSQLASAIAVMPSSQKGKEGTDGENLEQDLCTFLISRACKNSTLANYLYWYVIVECEDQDTQQRDPKTHDMYLNVMRRFSQALLKGDKSVRVMRSLLASQQTFVDRLVQLMKAVQRESGNRKKKTERLQSLLADNEKVNLSEIEPIPLPLEPQIRIKGIIPETATLFKSALMPAKLIFKTEDGEQYPVIFKHGDDLRQDQLILQIISLMDKLLRKENLDLKLTPYKVLATSTKHGFMQFVQSVPVAEVLATEGNIQSFFRKYAPNDKGPYGISSEVMDTYVKSCAGYCVITYILGVGDRHLDNLLLTKTGKLFHIDFGYILGRDPKPLPPPMKLSKEMVEGMGGMQSEQYQEFRKQCYTAFLHLRRYSNLILNLFSLMVDANIPDIALEPDKTVKKVQDKFRLDLSDEEAVHYMQSLIDESVGALFAAVVEQIHKFAQYWRR

Target ORF information:

RefSeq Version	XM_021477525.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphatidylinositol 3-kinase, catalytic subunit type 3 (pik3c3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477525.1

ORF Insert Sequence:

ATGGACACTG ACAAGTTTAA CTATGTGTAC AGTTGCGATT TGGACATAAA CGTTCAACTG 
AAAATAGGCA GTCTGGAGGG AAAGCGAGAG CAGAAGAGCT ACAAAGCTCT GCTTGAAGAC 
CCCATGCTGC GATTCTCAGG ACTCTATCAG GAGAGCTGCT CTGATCTGTA CGTCACCTGT 
CAGGTGTTTG CTGAGGGAAA ACCACTTGCC CTGCCTGTAC GAACCTCTTA TAAGGCCTTC 
AGCACTCGCT GGAACTGGAA TGAATGGCTC CGGCTGCCCG TGAAGTATCC TGACCTGCCT 
CAGAGTGCCC AGGTGACTCT GACCGTGTGG GACGTGTATG GACCTGGCCG CGCCACACCT 
GTTGGGGGCA CTACAGTCAC CTTGTTTGGA AAATATGGAA TGTTCCGGCA GGGCATGCAT 
GATCTGAAGG TGTGGCCGGG TGTGGAGGGA GATGGAACTG AGCCCACCAG TACACCAGGA 
CGGACCAGCA GCTCTCTGGC AGAAGACCAG ATGGGCCGCC TGGCCAAGGG CCCAGACCTG 
GAGATACTTA GTGACCTAAC AAAAGCTCAC CGTCAGGGAC ACATGGTTAA AGTGGACTGG 
CTGGATCGCT TGACCTTCCG AGAGATTGAG ATGATCAATG AGAGTGAGAA GCGTAGCTCT 
AATTTTATGT ACCTTATGGT TGAATTCCCT CGTGTCAAAA GTGGTGAACG TGAATACAGC 
ATTGTCTATT ACGAAAAGGA TGCAGATGAA TCCTCTCCTT TGCCGACCAG TTCAGATATT 
GTGAAAGTGC CTGACCCCCA GATGTGCATG GAAAATCTAG TGGAGAGTAA GCATCATAAA 
CTGGCCCGTA GTTTGCGCAG TGGACCTTCT GACCACGATC TCAAACCTAA CGCAGCCACA 
CGCGATCAAC TTAATATCAT AGTGAGCTAC CCACCTACAA AACAGCTGAG CTCTGAGGAA 
CAGGACCTTG TGTGGAAGTT TAGATACTAC CTCACCACAC AGGAGAAGGC TCTCACTAAG 
TTCCTGAAGT GTGTGAACTG GGATCTGCCT CAGGAGGCCA AACAGGCGCT GGAGCTACTG 
GGAAAGTGGC GTCCGATGGA TGTGGAGGAT TCACTGGAGC TGCTGTCGTC GCAGTTCACC 
AACCCCACCG TCAGACGCTA TGCCGTCGCC AGGCTACAGC AGGCCGATGA TGAGGATCTG 
CTGATGTACT TGCTCCAGTT GGTGCAGGCA TTGAAATATG AGAATTTCAA CGATATCCAG 
GGTGGTTTGG AGCCTGCCAA TAAAAGAGAC AATCAGGGGG GTTTGACCGA GAGCTCGACA 
ACAGGAGATC TGGACAGTTC TCAGTTGGCA TCAGCTATTG CAGTGATGCC CAGCTCACAA 
AAAGGCAAAG AGGGAACAGA TGGTGAAAAT CTAGAGCAAG ATCTGTGCAC CTTCCTTATA 
TCACGTGCCT GCAAAAACTC TACACTGGCA AACTATTTAT ACTGGTATGT GATAGTGGAG 
TGTGAGGATC AGGACACGCA GCAGAGAGAC CCAAAAACCC ATGACATGTA CCTCAATGTG 
ATGAGGCGCT TCAGTCAGGC TCTTCTTAAG GGTGATAAGA GTGTGCGAGT GATGCGTTCA 
CTTCTGGCCT CCCAGCAGAC GTTTGTAGAT AGACTGGTGC AGCTCATGAA AGCTGTTCAA 
AGAGAGAGCG GAAACCGCAA GAAAAAGACG GAGCGCTTAC AGAGCCTGTT GGCTGATAAT 
GAGAAGGTGA ATCTGTCAGA GATTGAGCCC ATTCCTCTCC CTCTGGAGCC ACAGATCCGC 
ATCAAAGGCA TCATTCCTGA GACCGCAACG CTCTTCAAGA GTGCTCTGAT GCCAGCAAAG 
CTCATCTTTA AGACTGAAGA TGGAGAGCAA TATCCTGTCA TCTTCAAACA TGGGGATGAC 
CTGCGGCAGG ACCAGCTCAT CCTGCAGATT ATCTCACTCA TGGACAAGTT GCTGAGGAAA 
GAGAATTTGG ACCTGAAGTT GACCCCATAC AAAGTTTTGG CGACCAGCAC CAAACACGGT 
TTTATGCAGT TTGTGCAGTC AGTGCCTGTG GCTGAGGTTT TAGCGACTGA AGGAAACATT 
CAGAGTTTCT TCAGAAAGTA CGCACCCAAT GATAAAGGAC CGTATGGCAT CAGCTCTGAG 
GTCATGGACA CGTATGTTAA AAGCTGTGCG GGTTATTGTG TGATCACCTA TATCCTCGGG 
GTCGGAGACC GACATCTAGA CAACCTGCTT CTCACCAAGA CTGGGAAGCT GTTTCATATA 
GATTTTGGGT ATATTTTGGG TCGTGACCCT AAACCCCTGC CGCCCCCTAT GAAGCTGAGC 
AAGGAAATGG TGGAGGGGAT GGGGGGCATG CAGAGCGAGC AATACCAGGA ATTCAGGAAA 
CAGTGCTACA CCGCCTTCCT GCACCTACGC AGATACTCCA ATCTCATTTT GAATCTGTTC 
TCTCTGATGG TGGATGCTAA TATTCCAGAC ATTGCGCTGG AGCCTGACAA AACCGTAAAA 
AAGGTTCAGG ACAAGTTTCG ATTGGACCTC TCGGATGAGG AGGCGGTGCA TTACATGCAG 
AGTCTGATTG ATGAGAGCGT TGGAGCATTG TTTGCTGCTG TGGTTGAACA GATACACAAG 
TTTGCACAGT ATTGGCGCAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Deficiency in class III PI3-kinase confers postnatal lethality with IBD-like features in zebrafish.
Nature communications9(1)2639(2018 07)
Zhao S,Xia J,Wu X,Zhang L,Wang P,Wang H,Li H,Wang X,Chen Y,Agnetti J,Li Y,Pei D,Shu X

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

The zebrafish gene map defines ancestral vertebrate chromosomes.
Genome research15(9)1307-14(2005 Sep)
Woods IG,Wilson C,Friedlander B,Chang P,Reyes DK,Nix R,Kelly PD,Chu F,Postlethwait JH,Talbot WS

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pik3c3 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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