glb1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	glb1
Entrez Gene ID	548343
Full Name	galactosidase, beta 1
Synonyms	zgc:110823
General protein information	Preferred Names galactosidase, beta 1 Names beta-galactosidase
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005160132.3	XP_005160189.1	beta-galactosidase isoform X1
NM_001017547.1	NP_001017547.1	beta-galactosidase precursor
XM_005160132.4	XP_005160189.2	beta-galactosidase isoform X1

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os05g0539400	NP_001056172.1
Homo sapiens (human)	GLB1	NP_001073279.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002056	XP_001689312.2
Canis lupus familiaris (dog)	GLB1	NP_001032730.1
Pan troglodytes (chimpanzee)	GLB1	XP_003950098.1
Macaca mulatta (Rhesus monkey)	LOC709355	XP_001097877.1
Bos taurus (cattle)	GLB1	NP_001030215.1
Mus musculus (house mouse)	Glb1	NP_033882.1
Rattus norvegicus (Norway rat)	Glb1	NP_001101662.1
Danio rerio (zebrafish)	glb1	NP_001017547.1
Caenorhabditis elegans (roundworm)	T19B10.3	NP_505849.2
Caenorhabditis elegans (roundworm)	H22K11.2	NP_509140.2
Arabidopsis thaliana (thale cress)	BGAL17	NP_565051.1
Gallus gallus (chicken)	GLB1	NP_001265076.1
Xenopus tropicalis (tropical clawed frog)	glb1l	NP_001015958.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Selective yolk deposition and mannose phosphorylation of lysosomal glycosidases in zebrafish.
Fan X, Klein M, Flanagan-Steet HR, Steet R
The Journal of biological chemistry285(43)32946-53(2010 Oct)

Molecular cloning and biochemical characterization of sialidases from zebrafish (Danio rerio).
Manzoni M, Colombi P, Papini N, Rubaga L, Tiso N, Preti A, Venerando B, Tettamanti G, Bresciani R, Argenton F, Borsani G, Monti E
The Biochemical journal408(3)395-406(2007 Dec)

Hematopoietic gene expression profile in zebrafish kidney marrow.
Song HD, Sun XJ, Deng M, Zhang GW, Zhou Y, Wu XY, Sheng Y, Chen Y, Ruan Z, Jiang CL, Fan HY, Zon LI, Kanki JP, Liu TX, Look AT, Chen Z
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following glb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the glb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa36559	NM_001017547.1 Latest version!	Danio rerio galactosidase, beta 1 (glb1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODa06209	XM_005160132.3 Latest version!	Danio rerio galactosidase, beta 1 (glb1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODa147241	XM_005160132.4 Latest version!	Danio rerio galactosidase, beta 1 (glb1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

glb1 ( NM_001017547.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001017547.1 Danio rerio galactosidase, beta 1 (glb1), mRNA.

Price & Availability↑

CloneID	ODa36559
Clone ID Related Accession (Same CDS sequence)	NM_001017547.1
Accession Version	NM_001017547.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549296000000
Organism	Danio rerio(zebrafish)
Product	beta-galactosidase precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC092166.1. ##Evidence-Data-START## Transcript exon combination :: BC092166.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCTCCCG TTGTCGGACT AGTCTCGGTT CTGCTCCTTA TCATACCAGC CACCACGTAC 
GCCACAACTC CCACTTTTTC TGTGGATTAC CATCGAAACT GCTTTCTTAA AGATGGAGAG 
CCTTTCCGCT ACATATCTGG CAGTATCCAC TACAGCCGGA TCCCCAGAGT CTATTGGAAA 
GACCGGCTAC TCAAGATGTA CATGGCTGGA TTGAACGCCA TCCAGACATA TGTGCCGTGG 
AACTTCCACG AAGCAGTTCC TGGTCAGTAC GACTTCAGTG GAGATCGAGA TCTGGAGCAA 
TTCCTCCAGC TCTGCCAAGA CATCGGCCTG CTGGTCATCA TGAGACCAGG ACCCTACATA 
TGTGCCGAGT GGGATATGGG TGGTTTGCCT GCCTGGCTGC TGAAAAAAAA GGATATTGTG 
CTTCGATCAT CTGATCCAGA TTACCTTGCA GCAGTGGACA AGTGGATGGG GAAGCTGTTG 
CCCATCATAA AGCGTTACCT GTACCAGAAT GGAGGCCCAA TCATTACCGT GCAGGTAGAG 
AATGAGTATG GCAGCTACTT CGCCTGTGAT TTTAACTACA TGCGTCATCT GAGCCAGTTG 
TTCCGGTTTT ATCTCGGGGA AGAGGCTGTG CTGTTCACTA CTGATGGGGC AGGTCTGGGT 
TACTTGAAAT GTGGATCCCT CCAGGGCCTC TACGCCACTG TTGACTTCGG CCCAGGTGCA 
AATGTAACCG CTGCGTTTGA AGCACAGAGG CATGTTGAGC CACGTGGGCC TTTGGTGAAC 
TCTGAGTTTT ATCCCGGCTG GTTGGATCAT TGGGGCGAAA AACACTCGGT TGTTCCCACA 
TCTGCTGTAG TCAAGACTCT CAATGAAATT CTGGAAATTG GAGCCAATGT CAACCTGTAT 
ATGTTCATCG GTGGAACTAA CTTTGGCTAT TGGAATGGTG CCAACACTCC TTATGGTCCT 
CAACCCACCA GCTATGACTA TGACTCTCCT CTTACAGAGG CTGGAGATCT CACTGAGAAA 
TATTTCGCCA TAAGGGAAGT CATCAAAATG TATAAAGATG TACCAGAAGG AATTCTGCCT 
CCATCAACAC CTAAATTTGC ATATGGAAAA GTGCAAATGA AGAAACTAAA GACTGTGTCA 
GAGACGTTAG ATGTGTTGTC TTTCTCCGGC CCTGTAAAAA CACTTTACCC TCCAACATTT 
ATTGAAATGA ATCAGGCGTT TGGGTTCGTG CTTTATCAAA CTGTGCTACC AGTTAACTGC 
GTGAAGCCCA CGCCGCTGTC GTCCCCTCTG AATGGAGTCC ATGATCGGGC ATATATTTCC 
ATTGATGGCA TTGCTGCTGG TATTTTGGAG AGGAACAAGG CACTCAGCAT CAATATAACA 
GGGAAAGCTG GCAGCCATGT GGACGTCCTG GTGGAGAACA TGGGCAGAAT CAACTACGGC 
AAAGGAATTA ATGACTTCAA GGGTCTGGTG TTTAATCTGA CGCTCGGTGC TGATGTTCTG 
GTTAACTGGA CTGTGTATTC CCTGAGTGTC GATGAAGCAG TCAGCAAGGG TTTGCTTTCA 
GCTTCAGGCT CCTCCTCCAC CAGCTCCACC AGCACACCTC CGCCTGCTCT TTCACCTCCA 
ACCTTCTACA CAGGCAGCTT CATCATTCCT GACGGTATCC CTGATCTCCC TCAGGACACC 
TACATCCAGT TCCCCGACTG GAGGAAGGGT CAAGTGTGGA TAAATGGATT TAACGTTGGC 
CGCTACTGGC CGTCCCGTGG GCCACAGATC ACTCTCTTTG TCCCGGCGCA CCTGCTCAGC 
ACCTCCGTCC AAAACAACAT AACCGTCCTG GAGCTTGAGG CGTCCCCTTG CTCCTCAGGT 
TCCTGCATGG TGGAGTTCAC TGATACTCCT ATTTTGAACG GCACCGTGAA CAGCCAATAC 
AAGAGACAAC TATTCAACAA ACAAGACCTC TTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001017547.1
CDS	18..1973
Translation	MAPVVGLVSVLLLIIPATTYATTPTFSVDYHRNCFLKDGEPFRYISGSIHYSRIPRVYWKDRLLKMYMAGLNAIQTYVPWNFHEAVPGQYDFSGDRDLEQFLQLCQDIGLLVIMRPGPYICAEWDMGGLPAWLLKKKDIVLRSSDPDYLAAVDKWMGKLLPIIKRYLYQNGGPIITVQVENEYGSYFACDFNYMRHLSQLFRFYLGEEAVLFTTDGAGLGYLKCGSLQGLYATVDFGPGANVTAAFEAQRHVEPRGPLVNSEFYPGWLDHWGEKHSVVPTSAVVKTLNEILEIGANVNLYMFIGGTNFGYWNGANTPYGPQPTSYDYDSPLTEAGDLTEKYFAIREVIKMYKDVPEGILPPSTPKFAYGKVQMKKLKTVSETLDVLSFSGPVKTLYPPTFIEMNQAFGFVLYQTVLPVNCVKPTPLSSPLNGVHDRAYISIDGIAAGILERNKALSINITGKAGSHVDVLVENMGRINYGKGINDFKGLVFNLTLGADVLVNWTVYSLSVDEAVSKGLLSASGSSSTSSTSTPPPALSPPTFYTGSFIIPDGIPDLPQDTYIQFPDWRKGQVWINGFNVGRYWPSRGPQITLFVPAHLLSTSVQNNITVLELEASPCSSGSCMVEFTDTPILNGTVNSQYKRQLFNKQDLL

Target ORF information:

RefSeq Version	NM_001017547.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio galactosidase, beta 1 (glb1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001017547.1

ORF Insert Sequence:

ATGGCTCCCG TTGTCGGACT AGTCTCGGTT CTGCTCCTTA TCATACCAGC CACCACGTAC 
GCCACAACTC CCACTTTTTC TGTGGATTAC CATCGAAACT GCTTTCTTAA AGATGGAGAG 
CCTTTCCGCT ACATATCTGG CAGTATCCAC TACAGCCGGA TCCCCAGAGT CTATTGGAAA 
GACCGGCTAC TCAAGATGTA CATGGCTGGA TTGAACGCCA TCCAGACATA TGTGCCGTGG 
AACTTCCACG AAGCAGTTCC TGGTCAGTAC GACTTCAGTG GAGATCGAGA TCTGGAGCAA 
TTCCTCCAGC TCTGCCAAGA CATCGGCCTG CTGGTCATCA TGAGACCAGG ACCCTACATA 
TGTGCCGAGT GGGATATGGG TGGTTTGCCT GCCTGGCTGC TGAAAAAAAA GGATATTGTG 
CTTCGATCAT CTGATCCAGA TTACCTTGCA GCAGTGGACA AGTGGATGGG GAAGCTGTTG 
CCCATCATAA AGCGTTACCT GTACCAGAAT GGAGGCCCAA TCATTACCGT GCAGGTAGAG 
AATGAGTATG GCAGCTACTT CGCCTGTGAT TTTAACTACA TGCGTCATCT GAGCCAGTTG 
TTCCGGTTTT ATCTCGGGGA AGAGGCTGTG CTGTTCACTA CTGATGGGGC AGGTCTGGGT 
TACTTGAAAT GTGGATCCCT CCAGGGCCTC TACGCCACTG TTGACTTCGG CCCAGGTGCA 
AATGTAACCG CTGCGTTTGA AGCACAGAGG CATGTTGAGC CACGTGGGCC TTTGGTGAAC 
TCTGAGTTTT ATCCCGGCTG GTTGGATCAT TGGGGCGAAA AACACTCGGT TGTTCCCACA 
TCTGCTGTAG TCAAGACTCT CAATGAAATT CTGGAAATTG GAGCCAATGT CAACCTGTAT 
ATGTTCATCG GTGGAACTAA CTTTGGCTAT TGGAATGGTG CCAACACTCC TTATGGTCCT 
CAACCCACCA GCTATGACTA TGACTCTCCT CTTACAGAGG CTGGAGATCT CACTGAGAAA 
TATTTCGCCA TAAGGGAAGT CATCAAAATG TATAAAGATG TACCAGAAGG AATTCTGCCT 
CCATCAACAC CTAAATTTGC ATATGGAAAA GTGCAAATGA AGAAACTAAA GACTGTGTCA 
GAGACGTTAG ATGTGTTGTC TTTCTCCGGC CCTGTAAAAA CACTTTACCC TCCAACATTT 
ATTGAAATGA ATCAGGCGTT TGGGTTCGTG CTTTATCAAA CTGTGCTACC AGTTAACTGC 
GTGAAGCCCA CGCCGCTGTC GTCCCCTCTG AATGGAGTCC ATGATCGGGC ATATATTTCC 
ATTGATGGCA TTGCTGCTGG TATTTTGGAG AGGAACAAGG CACTCAGCAT CAATATAACA 
GGGAAAGCTG GCAGCCATGT GGACGTCCTG GTGGAGAACA TGGGCAGAAT CAACTACGGC 
AAAGGAATTA ATGACTTCAA GGGTCTGGTG TTTAATCTGA CGCTCGGTGC TGATGTTCTG 
GTTAACTGGA CTGTGTATTC CCTGAGTGTC GATGAAGCAG TCAGCAAGGG TTTGCTTTCA 
GCTTCAGGCT CCTCCTCCAC CAGCTCCACC AGCACACCTC CGCCTGCTCT TTCACCTCCA 
ACCTTCTACA CAGGCAGCTT CATCATTCCT GACGGTATCC CTGATCTCCC TCAGGACACC 
TACATCCAGT TCCCCGACTG GAGGAAGGGT CAAGTGTGGA TAAATGGATT TAACGTTGGC 
CGCTACTGGC CGTCCCGTGG GCCACAGATC ACTCTCTTTG TCCCGGCGCA CCTGCTCAGC 
ACCTCCGTCC AAAACAACAT AACCGTCCTG GAGCTTGAGG CGTCCCCTTG CTCCTCAGGT 
TCCTGCATGG TGGAGTTCAC TGATACTCCT ATTTTGAACG GCACCGTGAA CAGCCAATAC 
AAGAGACAAC TATTCAACAA ACAAGACCTC TTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

glb1 ( XM_005160132.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160189.1 Danio rerio galactosidase, beta 1 (glb1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa06209
Clone ID Related Accession (Same CDS sequence)	XM_005160132.3
Accession Version	XM_005160132.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	beta-galactosidase isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160132.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCCCG GTGTCGGACT AGTCTCGGTT CTGCTCCTTA TCATACCAGC CACCACGTAC 
GCCACAACTC CCACTTTTTC TGTGGATTAC CATCGAAACT GCTTTCTTAA AGATGGAGAG 
CCATTCCGCT ACATATCTGG CAGTATCCAC TACAGCCGGA TCCCTAGAGT CTATTGGAAA 
GACCGGCTAC TCAAGATGTA CATGGCTGGA TTGAACGCCA TCCAGACATA TGTGCCGTGG 
AACTTCCACG AAGCAGTTCC TGGTCAGTAC GACTTCAGTG GAGATCGAGA TCTGGAGCAA 
TTCCTCCAGC TCTGCCAAGA CATCGGCCTG CTGGTCATCA TGAGACCAGG ACCCTACATA 
TGTGCCGAGT GGGATATGGG TGGTTTGCCT GCCTGGCTGC TGAAAAAAAA GGATATTGTG 
CTTCGATCAT CTGATCCAGA TTACCTTGCA GCAGTGGACA AGTGGATGGG GAAGCTGTTG 
CCAATCATAA AGCGTTACCT GTACCAGAAT GGAGGCCCAA TCATTACCGT GCAGGTAGAG 
AATGAGTACG GCAGCTACTT CGCCTGTGAT TTTAACTACA TGCGTCATCT GAGCCAGTTG 
TTCCGGTTTT ATCTCGGGGA AGAGGCTGTG CTGTTCACTA CTGATGGGGC AGGTCTGGGT 
TACTTGAAAT GTGGATCCCT CCAGGGCCTC TACGCCACTG TTGACTTCGG CCCAGGTGCA 
AATGTAACCG CTGCGTTTGA AGCACAGAGG CATGTTGAGC CACGTGGGCC TTTGGTGAAC 
TCTGAGTTTT ACCCCGGCTG GTTGGATCAT TGGGGCGAAA AACACTCGGT GGTTCCTACA 
TCTGCTGTAG TCAAGACTCT CAATGAAATT CTGGAAATTG GAGCCAATGT CAACCTGTAT 
ATGTTCATCG GTGGAACTAA CTTTGGCTAT TGGAATGGTG CCAACACTCC TTATGGTCCT 
CAACCCACCA GCTATGACTA TGACTCTCCT CTTACAGAGG CTGGAGATCT CACTGAGAAA 
TATTTCGCCA TAAGGGAAGT CATCAAAATG TATAAAGATG TACCAGAAGG AATTCTGCCT 
CCATCAACAC CTAAATTTGC ATATGGAAAA GTGCAAATGA AGAAACTAAA GACTGTGTCA 
GAGACGTTGG ATGTGTTGTC TTTCTCCGGC CCTGTAAAAA CACTTTACCC TCCAACATTT 
ATTGAAATGA ATCAGGCGTT TGGGTTCGTG CTTTATCAAA CTGTGCTACC AGTTAACTGC 
GTGAAGCCCA CGCCGCTGTC GTCCCCTCTG AATGGAGTCC ACGATCGGGC ATATATTTCC 
ATTGATGGCA CTGCTGCTGG TATTTTGGAG AGGAACAAGG CACTCAGCAT CAATATAACA 
GGGAAAGCTG GCAGCCGTGT GGACGTCCTG GTGGAGAACA TGGGCAGAAT CAACTACGGC 
AAAGGAATTA ATGACTTCAA GGGTCTGGTG TTTAATCTGA CACTCGGTGC TGATGTTCTG 
GTTAACTGGA CTGTGTATTC CCTGAGTGTC GATGAAGCAG TCAGCAAGGG TTTGCTTTTA 
GCTTCAGGCT CCTCCTCCAC CAGCTCCACC AGCACACCTC CGCCTGCTCT TTCACCTCCA 
ACCTTCTACA CAGGCAGCTT CATCATTCCT GACGGTATCC CTGATCTCCC TCAGGACACC 
TATATCCAGT TCCCCGACTG GAGGAAGGGT CAAGTGTGGA TAAATGGATT TAACGTTGGC 
CGCTACTGGC CGTCCCGTGG GCCACAGATC ACTCTCTTTG TCCCGGCGCA CCTGCTCAGC 
ACCTCCGTCC AAAACAACAT AACCGTCCTG GAGCTTGAGG CGTCTCCTTG CTCCTCAGGT 
TCCTGCATGG TGGAGTTCAC TGATACTCCT ATTTTAAACG GCACCGTGAA CGGCCAATAC 
AAGAGACAAC TATTCAACAA ACAAGACCTC TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160189.1
CDS	75..2030
Translation	MTPGVGLVSVLLLIIPATTYATTPTFSVDYHRNCFLKDGEPFRYISGSIHYSRIPRVYWKDRLLKMYMAGLNAIQTYVPWNFHEAVPGQYDFSGDRDLEQFLQLCQDIGLLVIMRPGPYICAEWDMGGLPAWLLKKKDIVLRSSDPDYLAAVDKWMGKLLPIIKRYLYQNGGPIITVQVENEYGSYFACDFNYMRHLSQLFRFYLGEEAVLFTTDGAGLGYLKCGSLQGLYATVDFGPGANVTAAFEAQRHVEPRGPLVNSEFYPGWLDHWGEKHSVVPTSAVVKTLNEILEIGANVNLYMFIGGTNFGYWNGANTPYGPQPTSYDYDSPLTEAGDLTEKYFAIREVIKMYKDVPEGILPPSTPKFAYGKVQMKKLKTVSETLDVLSFSGPVKTLYPPTFIEMNQAFGFVLYQTVLPVNCVKPTPLSSPLNGVHDRAYISIDGTAAGILERNKALSINITGKAGSRVDVLVENMGRINYGKGINDFKGLVFNLTLGADVLVNWTVYSLSVDEAVSKGLLLASGSSSTSSTSTPPPALSPPTFYTGSFIIPDGIPDLPQDTYIQFPDWRKGQVWINGFNVGRYWPSRGPQITLFVPAHLLSTSVQNNITVLELEASPCSSGSCMVEFTDTPILNGTVNGQYKRQLFNKQDLF

Target ORF information:

RefSeq Version	XM_005160132.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio galactosidase, beta 1 (glb1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160132.3

ORF Insert Sequence:

ATGACTCCCG GTGTCGGACT AGTCTCGGTT CTGCTCCTTA TCATACCAGC CACCACGTAC 
GCCACAACTC CCACTTTTTC TGTGGATTAC CATCGAAACT GCTTTCTTAA AGATGGAGAG 
CCATTCCGCT ACATATCTGG CAGTATCCAC TACAGCCGGA TCCCTAGAGT CTATTGGAAA 
GACCGGCTAC TCAAGATGTA CATGGCTGGA TTGAACGCCA TCCAGACATA TGTGCCGTGG 
AACTTCCACG AAGCAGTTCC TGGTCAGTAC GACTTCAGTG GAGATCGAGA TCTGGAGCAA 
TTCCTCCAGC TCTGCCAAGA CATCGGCCTG CTGGTCATCA TGAGACCAGG ACCCTACATA 
TGTGCCGAGT GGGATATGGG TGGTTTGCCT GCCTGGCTGC TGAAAAAAAA GGATATTGTG 
CTTCGATCAT CTGATCCAGA TTACCTTGCA GCAGTGGACA AGTGGATGGG GAAGCTGTTG 
CCAATCATAA AGCGTTACCT GTACCAGAAT GGAGGCCCAA TCATTACCGT GCAGGTAGAG 
AATGAGTACG GCAGCTACTT CGCCTGTGAT TTTAACTACA TGCGTCATCT GAGCCAGTTG 
TTCCGGTTTT ATCTCGGGGA AGAGGCTGTG CTGTTCACTA CTGATGGGGC AGGTCTGGGT 
TACTTGAAAT GTGGATCCCT CCAGGGCCTC TACGCCACTG TTGACTTCGG CCCAGGTGCA 
AATGTAACCG CTGCGTTTGA AGCACAGAGG CATGTTGAGC CACGTGGGCC TTTGGTGAAC 
TCTGAGTTTT ACCCCGGCTG GTTGGATCAT TGGGGCGAAA AACACTCGGT GGTTCCTACA 
TCTGCTGTAG TCAAGACTCT CAATGAAATT CTGGAAATTG GAGCCAATGT CAACCTGTAT 
ATGTTCATCG GTGGAACTAA CTTTGGCTAT TGGAATGGTG CCAACACTCC TTATGGTCCT 
CAACCCACCA GCTATGACTA TGACTCTCCT CTTACAGAGG CTGGAGATCT CACTGAGAAA 
TATTTCGCCA TAAGGGAAGT CATCAAAATG TATAAAGATG TACCAGAAGG AATTCTGCCT 
CCATCAACAC CTAAATTTGC ATATGGAAAA GTGCAAATGA AGAAACTAAA GACTGTGTCA 
GAGACGTTGG ATGTGTTGTC TTTCTCCGGC CCTGTAAAAA CACTTTACCC TCCAACATTT 
ATTGAAATGA ATCAGGCGTT TGGGTTCGTG CTTTATCAAA CTGTGCTACC AGTTAACTGC 
GTGAAGCCCA CGCCGCTGTC GTCCCCTCTG AATGGAGTCC ACGATCGGGC ATATATTTCC 
ATTGATGGCA CTGCTGCTGG TATTTTGGAG AGGAACAAGG CACTCAGCAT CAATATAACA 
GGGAAAGCTG GCAGCCGTGT GGACGTCCTG GTGGAGAACA TGGGCAGAAT CAACTACGGC 
AAAGGAATTA ATGACTTCAA GGGTCTGGTG TTTAATCTGA CACTCGGTGC TGATGTTCTG 
GTTAACTGGA CTGTGTATTC CCTGAGTGTC GATGAAGCAG TCAGCAAGGG TTTGCTTTTA 
GCTTCAGGCT CCTCCTCCAC CAGCTCCACC AGCACACCTC CGCCTGCTCT TTCACCTCCA 
ACCTTCTACA CAGGCAGCTT CATCATTCCT GACGGTATCC CTGATCTCCC TCAGGACACC 
TATATCCAGT TCCCCGACTG GAGGAAGGGT CAAGTGTGGA TAAATGGATT TAACGTTGGC 
CGCTACTGGC CGTCCCGTGG GCCACAGATC ACTCTCTTTG TCCCGGCGCA CCTGCTCAGC 
ACCTCCGTCC AAAACAACAT AACCGTCCTG GAGCTTGAGG CGTCTCCTTG CTCCTCAGGT 
TCCTGCATGG TGGAGTTCAC TGATACTCCT ATTTTAAACG GCACCGTGAA CGGCCAATAC 
AAGAGACAAC TATTCAACAA ACAAGACCTC TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

glb1 ( XM_005160132.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160189.2 Danio rerio galactosidase, beta 1 (glb1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa147241
Clone ID Related Accession (Same CDS sequence)	XM_005160132.4
Accession Version	XM_005160132.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	beta-galactosidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160132.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCTTA GCTTTAGCAG CAACCAAAAC AAATGGCATT TGACTTCCGC ACCGCTGTTT 
ACCGTTTCAA ACACCATGAC TCCCGGTGTC GGACTAGTCT CGGTTCTGCT CCTTATCATA 
CCAGCCACCA CGTACGCCAC AACTCCCACT TTTTCTGTGG ATTACCATCG AAACTGCTTT 
CTTAAAGATG GAGAGCCATT CCGCTACATA TCTGGCAGTA TCCACTACAG CCGGATCCCT 
AGAGTCTATT GGAAAGACCG GCTACTCAAG ATGTACATGG CTGGATTGAA CGCCATCCAG 
ACATATGTGC CGTGGAACTT CCACGAAGCA GTTCCTGGTC AGTACGACTT CAGTGGAGAT 
CGAGATCTGG AGCAATTCCT CCAGCTCTGC CAAGACATCG GCCTGCTGGT CATCATGAGA 
CCAGGACCCT ACATATGTGC CGAGTGGGAT ATGGGTGGTT TGCCTGCCTG GCTGCTGAAA 
AAAAAGGATA TTGTGCTTCG ATCATCTGAT CCAGATTACC TTGCAGCAGT GGACAAGTGG 
ATGGGGAAGC TGTTGCCAAT CATAAAGCGT TACCTGTACC AGAATGGAGG CCCAATCATT 
ACCGTGCAGG TAGAGAATGA GTACGGCAGC TACTTCGCCT GTGATTTTAA CTACATGCGT 
CATCTGAGCC AGTTGTTCCG GTTTTATCTC GGGGAAGAGG CTGTGCTGTT CACTACTGAT 
GGGGCAGGTC TGGGTTACTT GAAATGTGGA TCCCTCCAGG GCCTCTACGC CACTGTTGAC 
TTCGGCCCAG GTGCAAATGT AACCGCTGCG TTTGAAGCAC AGAGGCATGT TGAGCCACGT 
GGGCCTTTGG TGAACTCTGA GTTTTACCCC GGCTGGTTGG ATCATTGGGG CGAAAAACAC 
TCGGTGGTTC CTACATCTGC TGTAGTCAAG ACTCTCAATG AAATTCTGGA AATTGGAGCC 
AATGTCAACC TGTATATGTT CATCGGTGGA ACTAACTTTG GCTATTGGAA TGGTGCCAAC 
ACTCCTTATG GTCCTCAACC CACCAGCTAT GACTATGACT CTCCTCTTAC AGAGGCTGGA 
GATCTCACTG AGAAATATTT CGCCATAAGG GAAGTCATCA AAATGTATAA AGATGTACCA 
GAAGGAATTC TGCCTCCATC AACACCTAAA TTTGCATATG GAAAAGTGCA AATGAAGAAA 
CTAAAGACTG TGTCAGAGAC GTTGGATGTG TTGTCTTTCT CCGGCCCTGT AAAAACACTT 
TACCCTCCAA CATTTATTGA AATGAATCAG GCGTTTGGGT TCGTGCTTTA TCAAACTGTG 
CTACCAGTTA ACTGCGTGAA GCCCACGCCG CTGTCGTCCC CTCTGAATGG AGTCCACGAT 
CGGGCATATA TTTCCATTGA TGGCACTGCT GCTGGTATTT TGGAGAGGAA CAAGGCACTC 
AGCATCAATA TAACAGGGAA AGCTGGCAGC CGTGTGGACG TCCTGGTGGA GAACATGGGC 
AGAATCAACT ACGGCAAAGG AATTAATGAC TTCAAGGGTC TGGTGTTTAA TCTGACACTC 
GGTGCTGATG TTCTGGTTAA CTGGACTGTG TATTCCCTGA GTGTCGATGA AGCAGTCAGC 
AAGGGTTTGC TTTTAGCTTC AGGCTCCTCC TCCACCAGCT CCACCAGCAC ACCTCCGCCT 
GCTCTTTCAC CTCCAACCTT CTACACAGGC AGCTTCATCA TTCCTGACGG TATCCCTGAT 
CTCCCTCAGG ACACCTATAT CCAGTTCCCC GACTGGAGGA AGGGTCAAGT GTGGATAAAT 
GGATTTAACG TTGGCCGCTA CTGGCCGTCC CGTGGGCCAC AGATCACTCT CTTTGTCCCG 
GCGCACCTGC TCAGCACCTC CGTCCAAAAC AACATAACCG TCCTGGAGCT TGAGGCGTCT 
CCTTGCTCCT CAGGTTCCTG CATGGTGGAG TTCACTGATA CTCCTATTTT AAACGGCACC 
GTGAACGGCC AATACAAGAG ACAACTATTC AACAAACAAG ACCTCTTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160189.2
CDS	14..2044
Translation	MILSFSSNQNKWHLTSAPLFTVSNTMTPGVGLVSVLLLIIPATTYATTPTFSVDYHRNCFLKDGEPFRYISGSIHYSRIPRVYWKDRLLKMYMAGLNAIQTYVPWNFHEAVPGQYDFSGDRDLEQFLQLCQDIGLLVIMRPGPYICAEWDMGGLPAWLLKKKDIVLRSSDPDYLAAVDKWMGKLLPIIKRYLYQNGGPIITVQVENEYGSYFACDFNYMRHLSQLFRFYLGEEAVLFTTDGAGLGYLKCGSLQGLYATVDFGPGANVTAAFEAQRHVEPRGPLVNSEFYPGWLDHWGEKHSVVPTSAVVKTLNEILEIGANVNLYMFIGGTNFGYWNGANTPYGPQPTSYDYDSPLTEAGDLTEKYFAIREVIKMYKDVPEGILPPSTPKFAYGKVQMKKLKTVSETLDVLSFSGPVKTLYPPTFIEMNQAFGFVLYQTVLPVNCVKPTPLSSPLNGVHDRAYISIDGTAAGILERNKALSINITGKAGSRVDVLVENMGRINYGKGINDFKGLVFNLTLGADVLVNWTVYSLSVDEAVSKGLLLASGSSSTSSTSTPPPALSPPTFYTGSFIIPDGIPDLPQDTYIQFPDWRKGQVWINGFNVGRYWPSRGPQITLFVPAHLLSTSVQNNITVLELEASPCSSGSCMVEFTDTPILNGTVNGQYKRQLFNKQDLF

Target ORF information:

RefSeq Version	XM_005160132.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio galactosidase, beta 1 (glb1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160132.4

ORF Insert Sequence:

ATGATTCTTA GCTTTAGCAG CAACCAAAAC AAATGGCATT TGACTTCCGC ACCGCTGTTT 
ACCGTTTCAA ACACCATGAC TCCCGGTGTC GGACTAGTCT CGGTTCTGCT CCTTATCATA 
CCAGCCACCA CGTACGCCAC AACTCCCACT TTTTCTGTGG ATTACCATCG AAACTGCTTT 
CTTAAAGATG GAGAGCCATT CCGCTACATA TCTGGCAGTA TCCACTACAG CCGGATCCCT 
AGAGTCTATT GGAAAGACCG GCTACTCAAG ATGTACATGG CTGGATTGAA CGCCATCCAG 
ACATATGTGC CGTGGAACTT CCACGAAGCA GTTCCTGGTC AGTACGACTT CAGTGGAGAT 
CGAGATCTGG AGCAATTCCT CCAGCTCTGC CAAGACATCG GCCTGCTGGT CATCATGAGA 
CCAGGACCCT ACATATGTGC CGAGTGGGAT ATGGGTGGTT TGCCTGCCTG GCTGCTGAAA 
AAAAAGGATA TTGTGCTTCG ATCATCTGAT CCAGATTACC TTGCAGCAGT GGACAAGTGG 
ATGGGGAAGC TGTTGCCAAT CATAAAGCGT TACCTGTACC AGAATGGAGG CCCAATCATT 
ACCGTGCAGG TAGAGAATGA GTACGGCAGC TACTTCGCCT GTGATTTTAA CTACATGCGT 
CATCTGAGCC AGTTGTTCCG GTTTTATCTC GGGGAAGAGG CTGTGCTGTT CACTACTGAT 
GGGGCAGGTC TGGGTTACTT GAAATGTGGA TCCCTCCAGG GCCTCTACGC CACTGTTGAC 
TTCGGCCCAG GTGCAAATGT AACCGCTGCG TTTGAAGCAC AGAGGCATGT TGAGCCACGT 
GGGCCTTTGG TGAACTCTGA GTTTTACCCC GGCTGGTTGG ATCATTGGGG CGAAAAACAC 
TCGGTGGTTC CTACATCTGC TGTAGTCAAG ACTCTCAATG AAATTCTGGA AATTGGAGCC 
AATGTCAACC TGTATATGTT CATCGGTGGA ACTAACTTTG GCTATTGGAA TGGTGCCAAC 
ACTCCTTATG GTCCTCAACC CACCAGCTAT GACTATGACT CTCCTCTTAC AGAGGCTGGA 
GATCTCACTG AGAAATATTT CGCCATAAGG GAAGTCATCA AAATGTATAA AGATGTACCA 
GAAGGAATTC TGCCTCCATC AACACCTAAA TTTGCATATG GAAAAGTGCA AATGAAGAAA 
CTAAAGACTG TGTCAGAGAC GTTGGATGTG TTGTCTTTCT CCGGCCCTGT AAAAACACTT 
TACCCTCCAA CATTTATTGA AATGAATCAG GCGTTTGGGT TCGTGCTTTA TCAAACTGTG 
CTACCAGTTA ACTGCGTGAA GCCCACGCCG CTGTCGTCCC CTCTGAATGG AGTCCACGAT 
CGGGCATATA TTTCCATTGA TGGCACTGCT GCTGGTATTT TGGAGAGGAA CAAGGCACTC 
AGCATCAATA TAACAGGGAA AGCTGGCAGC CGTGTGGACG TCCTGGTGGA GAACATGGGC 
AGAATCAACT ACGGCAAAGG AATTAATGAC TTCAAGGGTC TGGTGTTTAA TCTGACACTC 
GGTGCTGATG TTCTGGTTAA CTGGACTGTG TATTCCCTGA GTGTCGATGA AGCAGTCAGC 
AAGGGTTTGC TTTTAGCTTC AGGCTCCTCC TCCACCAGCT CCACCAGCAC ACCTCCGCCT 
GCTCTTTCAC CTCCAACCTT CTACACAGGC AGCTTCATCA TTCCTGACGG TATCCCTGAT 
CTCCCTCAGG ACACCTATAT CCAGTTCCCC GACTGGAGGA AGGGTCAAGT GTGGATAAAT 
GGATTTAACG TTGGCCGCTA CTGGCCGTCC CGTGGGCCAC AGATCACTCT CTTTGTCCCG 
GCGCACCTGC TCAGCACCTC CGTCCAAAAC AACATAACCG TCCTGGAGCT TGAGGCGTCT 
CCTTGCTCCT CAGGTTCCTG CATGGTGGAG TTCACTGATA CTCCTATTTT AAACGGCACC 
GTGAACGGCC AATACAAGAG ACAACTATTC AACAAACAAG ACCTCTTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Selective yolk deposition and mannose phosphorylation of lysosomal glycosidases in zebrafish.
The Journal of biological chemistry285(43)32946-53(2010 Oct)
Fan X,Klein M,Flanagan-Steet HR,Steet R

Molecular cloning and biochemical characterization of sialidases from zebrafish (Danio rerio).
The Biochemical journal408(3)395-406(2007 Dec)
Manzoni M,Colombi P,Papini N,Rubaga L,Tiso N,Preti A,Venerando B,Tettamanti G,Bresciani R,Argenton F,Borsani G,Monti E

Hematopoietic gene expression profile in zebrafish kidney marrow.
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)
Song HD,Sun XJ,Deng M,Zhang GW,Zhou Y,Wu XY,Sheng Y,Chen Y,Ruan Z,Jiang CL,Fan HY,Zon LI,Kanki JP,Liu TX,Look AT,Chen Z

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glb1 cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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