SEMA3FA cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	SEMA3FA
Entrez Gene ID	544658
Full Name	sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fa
Synonyms	wu:fb92d12
General protein information	Preferred Names sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fa Names sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fa extracellular signal molecule sema3f1 semaphorin 3fa
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001014822.1	NP_001014822.1	sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fa
XM_005166040.2	XP_005166097.1	sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fa isoform X2
XM_005166039.3	XP_005166096.1	sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fa isoform X1
XM_005166040.3	XP_005166097.1	sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fa isoform X2
XM_005166039.2	XP_005166096.1	sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fa isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SEMA3F	XP_003309850.2
Macaca mulatta (Rhesus monkey)	SEMA3F	XP_001103281.1
Rattus norvegicus (Norway rat)	Sema3f	NP_001101655.1
Danio rerio (zebrafish)	sema3fb	XP_001921106.2
Canis lupus familiaris (dog)	SEMA3F	XP_541879.1
Mus musculus (house mouse)	Sema3f	NP_035479.2
Xenopus tropicalis (tropical clawed frog)	sema3f	NP_001011157.1
Homo sapiens (human)	SEMA3F	NP_004177.3
Bos taurus (cattle)	SEMA3F	XP_002697079.1
Danio rerio (zebrafish)	sema3fa	NP_001014822.1
Gallus gallus (chicken)	SEMA3F	NP_989589.1

Gene Ontology
Bibliography

Related articles in PubMed

Semaphorin/neuropilin binding specificities are stable over 400 million years of evolution.
He Z, Crenshaw E, Raper JA
Biochemical and biophysical research communications517(1)23-28(2019 Sep)

Human Semaphorin 3 Variants Link Melanocortin Circuit Development and Energy Balance.
van der Klaauw AA, Croizier S, Mendes de Oliveira E, Stadler LKJ, Park S, Kong Y, Banton MC, Tandon P, Hendricks AE, Keogh JM, Riley SE, Papadia S, Henning E, Bounds R, Bochukova EG, Mistry V, O'Rahilly S, Simerly RB, , , Minchin JEN, Barroso I, Jones EY, Bouret SG, Farooqi IS
Cell176(4)729-742.e18(2019 02)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Attractant and repellent cues cooperate in guiding a subset of olfactory sensory axons to a well-defined protoglomerular target.
Taku AA, Marcaccio CL, Ye W, Krause GJ, Raper JA
Development (Cambridge, England)143(1)123-32(2016 Jan)

Methyl-CpG Binding Protein 2 (Mecp2) Regulates Sensory Function Through Sema5b and Robo2.
Leong WY, Lim ZH, Korzh V, Pietri T, Goh EL
Frontiers in cellular neuroscience9481(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SEMA3FA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEMA3FA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa03131	NM_001014822.1 Latest version!	Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fa (sema3fa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa12050	XM_005166039.3 Latest version!	Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fa (sema3fa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa12051	XM_005166040.3 Latest version!	Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fa (sema3fa), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
ODa12051	XM_005166040.2	Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fa (sema3fa), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa12050	XM_005166039.2	Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fa (sema3fa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SEMA3FA ( NM_001014822.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001014822.1 Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fa (sema3fa), mRNA.

Price & Availability↑

CloneID	ODa03131
Clone ID Related Accession (Same CDS sequence)	NM_001014822.1
Accession Version	NM_001014822.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2391bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576252800000
Organism	Danio rerio(zebrafish)
Product	sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fa
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY766118.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY766118.1, BC163764.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGCAGGGAG CCGGGACTTT GGTGCTGGGG ACCCTTCTGG TCTCTGTATT CTCAGCTGTC 
TCGGTCCACA GCAGCCCTTT GTCTTCCCTG CCACCCGCCT CTGTTCCACG AGTCTTCCTG 
TCCTTCAAAG AGCTGAAGTC TACTGGAACA GCCCATCACT TTGCCCTCCT GCTGAACTCG 
ACGGACTACA GAATCCTCCG CATGGACGAG GATCATGATC GCATGTATGT GGGCAGCAAG 
GATTACATTC TGTCTCTAGA CCTGAATGAC ATCAACAAAG AGCCCCTTAT AATTCACTGG 
CCTGTAGCTC CGCAGAAAAG GACTGAATGT GTACTCTCAG GGAAAGACAT CAATGGCGAA 
TGTGGTAATT TCATCCGTCT AATCGAGCCG TGGAACCGGA CTCACCTGTA TGTGTGCGGG 
ACGGGAGCTT ACAACCCCAT CTGCACCTAT GTGGACAGAG GCCAAAGGGC ACAGGGTTTT 
CTGCAAGATC AGATGCCTCG TGCAGGAGGC CGAACCAGTC GAGCTGCTGA CCCTAGTGCC 
TCACCAGAAC CATATGCACC CAAGGAATAC ATTTTCCGAC TGGAGCCTGG TAAAGTAGAT 
TCTGGGAAAG GAAAATGCCC CTACGACCCC AAACTCAACA GCGTCTCAGC TTTGATAAAT 
GGCCAGCTTT ATGCCGGCGT CTACATCGAT TTTATGGGAA CAGACTCTGC CATCTTTCGC 
ACGTTGGGCA AACACACGGC AATGAGGACC GACCAGTATA ACTCCCGCTG GCTCCACGAT 
CCAACATTTG TCCATGCTCA GCTCATCCCT GATAGTGCAG AAAAGAATGA CGACAAGCTG 
TATTTCTTCT TCCGTGAGAA GGCCTCTGAG ATGGGTCAGA CTCCCATGGC TCAATCTAGG 
ATTGGCAGAA TTTGCCTGAA TGATGATGGA GGACACTGCT GTCTGGTGAA CAAGTGGAGC 
ACCTTCCTGA AGGCTCGGCT CATCTACTCT GTCACCGGCT CTGATGGCAT CGAGACGCAT 
TTCGATGAGC TCAGGGACGT GTACATCCAG AAGACTCAAG ACACTAAGAA TCCTGTCATC 
TACGGCGTCT TCTCCGTGTC CGGGTCTGTG TTTAAAGGCT CAGCCGTATG TGTTTATTCA 
ATGGCTGATG TGCGCATGGT CTTCAATGGG CCTTTCGCTC ATAAAGAAGG CCCAAACTAC 
CAATGGGTTG CATACCAGGG CAAAATCCCA TACCCTCGCC CAGGAACATG TCCAGGAGGA 
ACGTTCACCC CCAACATGAA GTCTACTAAA GATTACCCAG ATGAGGTGAT CAACTTCATG 
CGCACACATC CCACCATGTT CAATGCAGTT TATCCAGTCC ACAAGCGCCC CCTGGTGGTA 
CGAACCAACG TGGATTATGA GTTCACCACC ATCACTGTGG ACCAAGTGGC TGCAGCTGAT 
GGCAACTATG AAGTTCTGTT CTTAGGAACA GACAGGGGAA CAGTTCAGAA AGTGATTGTC 
CTGCCCAGAG ATGATCTACA GACTGAAGAG CTGGTTTTGG AGGAGGTGGA GGTCTTCAGA 
GTTCCAACTT CAATCACTAC TATGAAGATT TCTCCAAAAC GACAACAGCT GTATGTCAGT 
TCGGGGGTGG GCGTCACACA TCTGGCTCTG CACAGATGTG ATGTTTACGG AGAGGCCTGT 
GCCGACTGCT GCCTGGCCAG AGACCCATAC TGTGCCTGGG ACGGAAAGTC CTGCTCTCGC 
TACTCAGCAA ACCAAAAAAG GCGGAGTCGA AGGCAAGACG TGAAGTATGG AAACCCTATA 
AGGCAATGCA GAGGCTACAA CTCGAATGCC AATAAGAACA CATTGGAGAC AGTGCAGTAT 
GGAGTAGAGG GAAGCACAGC CTTTTTGGAG TGTCAGGCCA GATCTCCTCA TGTGTCCATC 
AAGTGTCATT TCCAGAAGGA GAACAGCGAC CGGAGAAGAG AGATCCGTTC TGACGGGCGT 
GTGGTTCGCA CGGAACAGGG CCTCCTCCTG CGCTCCCTCC AGCTGTCTGA CAGCGGTGTT 
TACCAGTGCA CCTCAACCGA GAAGAACTTC AAACACACCC TTGTCAAACT CCAGCTCGTG 
GTTCTCTCCG CCCACACAGT CAGCAGCATC CAGACTGAAA GCGGCGCCCC GGCCGCCCCA 
CCCCTTCAGT CCAGCGCCTG GGCGCCCAGC GCAGAACAAT ACAAAGACCT CCTGACCATC 
CTCAGCCAAC CTGAGATGGG CCTCATCAAC CAGTACTGCC AAGACTACTG GCAGATAGGG 
GATGACCCCA TGGCCCACAG GAAGAAGGAC CTCAAGGAGC TCCAGAAGGA CCTGCGGAAG 
CCCCGAAATA GGAGACACCA CCAGGAGCAG AGCAGCATGG CTGAGACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014822.1
CDS	1..2391
Translation	MQGAGTLVLGTLLVSVFSAVSVHSSPLSSLPPASVPRVFLSFKELKSTGTAHHFALLLNSTDYRILRMDEDHDRMYVGSKDYILSLDLNDINKEPLIIHWPVAPQKRTECVLSGKDINGECGNFIRLIEPWNRTHLYVCGTGAYNPICTYVDRGQRAQGFLQDQMPRAGGRTSRAADPSASPEPYAPKEYIFRLEPGKVDSGKGKCPYDPKLNSVSALINGQLYAGVYIDFMGTDSAIFRTLGKHTAMRTDQYNSRWLHDPTFVHAQLIPDSAEKNDDKLYFFFREKASEMGQTPMAQSRIGRICLNDDGGHCCLVNKWSTFLKARLIYSVTGSDGIETHFDELRDVYIQKTQDTKNPVIYGVFSVSGSVFKGSAVCVYSMADVRMVFNGPFAHKEGPNYQWVAYQGKIPYPRPGTCPGGTFTPNMKSTKDYPDEVINFMRTHPTMFNAVYPVHKRPLVVRTNVDYEFTTITVDQVAAADGNYEVLFLGTDRGTVQKVIVLPRDDLQTEELVLEEVEVFRVPTSITTMKISPKRQQLYVSSGVGVTHLALHRCDVYGEACADCCLARDPYCAWDGKSCSRYSANQKRRSRRQDVKYGNPIRQCRGYNSNANKNTLETVQYGVEGSTAFLECQARSPHVSIKCHFQKENSDRRREIRSDGRVVRTEQGLLLRSLQLSDSGVYQCTSTEKNFKHTLVKLQLVVLSAHTVSSIQTESGAPAAPPLQSSAWAPSAEQYKDLLTILSQPEMGLINQYCQDYWQIGDDPMAHRKKDLKELQKDLRKPRNRRHHQEQSSMAET

Target ORF information:

RefSeq Version	NM_001014822.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fa (sema3fa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014822.1

ORF Insert Sequence:

ATGCAGGGAG CCGGGACTTT GGTGCTGGGG ACCCTTCTGG TCTCTGTATT CTCAGCTGTC 
TCGGTCCACA GCAGCCCTTT GTCTTCCCTG CCACCCGCCT CTGTTCCACG AGTCTTCCTG 
TCCTTCAAAG AGCTGAAGTC TACTGGAACA GCCCATCACT TTGCCCTCCT GCTGAACTCG 
ACGGACTACA GAATCCTCCG CATGGACGAG GATCATGATC GCATGTATGT GGGCAGCAAG 
GATTACATTC TGTCTCTAGA CCTGAATGAC ATCAACAAAG AGCCCCTTAT AATTCACTGG 
CCTGTAGCTC CGCAGAAAAG GACTGAATGT GTACTCTCAG GGAAAGACAT CAATGGCGAA 
TGTGGTAATT TCATCCGTCT AATCGAGCCG TGGAACCGGA CTCACCTGTA TGTGTGCGGG 
ACGGGAGCTT ACAACCCCAT CTGCACCTAT GTGGACAGAG GCCAAAGGGC ACAGGGTTTT 
CTGCAAGATC AGATGCCTCG TGCAGGAGGC CGAACCAGTC GAGCTGCTGA CCCTAGTGCC 
TCACCAGAAC CATATGCACC CAAGGAATAC ATTTTCCGAC TGGAGCCTGG TAAAGTAGAT 
TCTGGGAAAG GAAAATGCCC CTACGACCCC AAACTCAACA GCGTCTCAGC TTTGATAAAT 
GGCCAGCTTT ATGCCGGCGT CTACATCGAT TTTATGGGAA CAGACTCTGC CATCTTTCGC 
ACGTTGGGCA AACACACGGC AATGAGGACC GACCAGTATA ACTCCCGCTG GCTCCACGAT 
CCAACATTTG TCCATGCTCA GCTCATCCCT GATAGTGCAG AAAAGAATGA CGACAAGCTG 
TATTTCTTCT TCCGTGAGAA GGCCTCTGAG ATGGGTCAGA CTCCCATGGC TCAATCTAGG 
ATTGGCAGAA TTTGCCTGAA TGATGATGGA GGACACTGCT GTCTGGTGAA CAAGTGGAGC 
ACCTTCCTGA AGGCTCGGCT CATCTACTCT GTCACCGGCT CTGATGGCAT CGAGACGCAT 
TTCGATGAGC TCAGGGACGT GTACATCCAG AAGACTCAAG ACACTAAGAA TCCTGTCATC 
TACGGCGTCT TCTCCGTGTC CGGGTCTGTG TTTAAAGGCT CAGCCGTATG TGTTTATTCA 
ATGGCTGATG TGCGCATGGT CTTCAATGGG CCTTTCGCTC ATAAAGAAGG CCCAAACTAC 
CAATGGGTTG CATACCAGGG CAAAATCCCA TACCCTCGCC CAGGAACATG TCCAGGAGGA 
ACGTTCACCC CCAACATGAA GTCTACTAAA GATTACCCAG ATGAGGTGAT CAACTTCATG 
CGCACACATC CCACCATGTT CAATGCAGTT TATCCAGTCC ACAAGCGCCC CCTGGTGGTA 
CGAACCAACG TGGATTATGA GTTCACCACC ATCACTGTGG ACCAAGTGGC TGCAGCTGAT 
GGCAACTATG AAGTTCTGTT CTTAGGAACA GACAGGGGAA CAGTTCAGAA AGTGATTGTC 
CTGCCCAGAG ATGATCTACA GACTGAAGAG CTGGTTTTGG AGGAGGTGGA GGTCTTCAGA 
GTTCCAACTT CAATCACTAC TATGAAGATT TCTCCAAAAC GACAACAGCT GTATGTCAGT 
TCGGGGGTGG GCGTCACACA TCTGGCTCTG CACAGATGTG ATGTTTACGG AGAGGCCTGT 
GCCGACTGCT GCCTGGCCAG AGACCCATAC TGTGCCTGGG ACGGAAAGTC CTGCTCTCGC 
TACTCAGCAA ACCAAAAAAG GCGGAGTCGA AGGCAAGACG TGAAGTATGG AAACCCTATA 
AGGCAATGCA GAGGCTACAA CTCGAATGCC AATAAGAACA CATTGGAGAC AGTGCAGTAT 
GGAGTAGAGG GAAGCACAGC CTTTTTGGAG TGTCAGGCCA GATCTCCTCA TGTGTCCATC 
AAGTGTCATT TCCAGAAGGA GAACAGCGAC CGGAGAAGAG AGATCCGTTC TGACGGGCGT 
GTGGTTCGCA CGGAACAGGG CCTCCTCCTG CGCTCCCTCC AGCTGTCTGA CAGCGGTGTT 
TACCAGTGCA CCTCAACCGA GAAGAACTTC AAACACACCC TTGTCAAACT CCAGCTCGTG 
GTTCTCTCCG CCCACACAGT CAGCAGCATC CAGACTGAAA GCGGCGCCCC GGCCGCCCCA 
CCCCTTCAGT CCAGCGCCTG GGCGCCCAGC GCAGAACAAT ACAAAGACCT CCTGACCATC 
CTCAGCCAAC CTGAGATGGG CCTCATCAAC CAGTACTGCC AAGACTACTG GCAGATAGGG 
GATGACCCCA TGGCCCACAG GAAGAAGGAC CTCAAGGAGC TCCAGAAGGA CCTGCGGAAG 
CCCCGAAATA GGAGACACCA CCAGGAGCAG AGCAGCATGG CTGAGACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA3FA ( XM_005166039.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005166096.1 Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fa (sema3fa), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa12050
Clone ID Related Accession (Same CDS sequence)	XM_005166039.3 , XM_005166039.2
Accession Version	XM_005166039.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fa isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005166039.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAG TGATCATGCA GGGAGCCGGG ACTTTGGTGC TGGGGACCCT TCTGGTCTCT 
GTATTCTCAG CTGTCTCGGT CCACAGCAGC CCTTTGTCTT CCCTGCCACC CGCCTCTGTT 
CCACGAGTCT TCCTGTCCTT CAAAGAGCTG AAGTCTACTG GAACAGCCCA TCACTTTGCC 
TTCCTGCTGA ACTCGACGGA CTACAGAATC CTCCGCATGG ACGAGGATCA TGATCGCATG 
TATGTGGGCA GCAAGGATTA CATTCTGTCT CTAGACCTGA ATGACATCAA CAAAGAGCCC 
CTTATAATTC ACTGGCCTGT AGCTCCACAG AAAAGGACTG AATGTGTACT CTCAGGGAAA 
GACATCAATG GCGAATGTGG TAATTTCATC CGTCTAATCG AGCCGTGGAA CCGGACTCAC 
CTGTATGTGT GCGGGACGGG AGCTTACAAC CCCATCTGCA CCTATGTGGA CAGAGGCCAA 
AGGGCACAGG GTTTTCTGCA AGATCAGATG CCTCGTGCAG GAGGCCGAAC CAGTCGAGCT 
GCTGACCCTA GTGCCTCACC AGAACCATAT GCACCCAAGG AATACATTTT CCGACTGGAG 
CCTGGTAAAG TAGATTCTGG GAAAGGAAAA TGCCCCTACG ACCCCAAACT CAACAGCGTC 
TCAGCTTTGA TAAATGGCCA GCTTTATGCC GGCGTCTACA TCGATTTTAT GGGAACAGAC 
TCTGCCATCT TTCGCACGTT GGGCAAACAC ACGGCAATGA GGACCGACCA GTATAACTCC 
CGCTGGCTCC ACGATCCAAC ATTTGTCCAT GCTCAGCTCA TCCCTGATAG TGCAGAAAAG 
AATGACGACA AGCTGTATTT CTTCTTCCGT GAGAAGGCCT CTGAGATGGG TCAGACTCCC 
ATGGCTCAAT CTAGGATTGG CAGAATTTGC CTGAATGATG ATGGAGGACA CTGCTGTCTG 
GTGAACAAGT GGAGCACCTT CCTGAAGGCT CGGCTCATCT GCTCTGTCAC CGGCTCTGAT 
GGCATCGAGA CGCATTTCGA TGAGCTCAGG GACGTGTACA TCCAGAAGAC TCAAGACACA 
AAGAATCCTG TCATCTACGG CGTCTTTTCC GTGTCCGGGT CTGTGTTTAA AGGCTCAGCA 
GTATGTGTTT ATTCAATGGC TGATGTGCGC ATGGTCTTCA ATGGGCCTTT CGCTCATAAA 
GAAGGCCCAA ACTACCAATG GGTTGCATAC CAGGGCAAAA TCCCATACCC TCGCCCAGGA 
ACATGTCCAG GAGGGACGTT TACCCCCAAC ATGAAGTCTA CTAAAGATTA CCCAGATGAG 
GTGATCAACT TCATGCGCAC ACATCCCACC ATGTTCAATG CAGTTTATCC AGTCCACAAG 
CGCCCCCTGG TGGTACGAAC CAACGTGGAT TATGAGTTCA CCACCATCAC TGTGGACCAA 
GTGGCTGCAG CTGATGGCAA CTATGAAGTT CTGTTCTTAG GAACAGACAG GGGAACAGTT 
CAGAAAGTGA TTGTCCTGCC CAGAGATGAT CTACAGACTG AAGAGCTGGT TTTGGAGGAG 
GTGGAGGTCT TCAGAGTTCC AACTTCAATC ACTACTATGA AGATTTCTCC AAAACGACAA 
CAGCTGTATG TCAGTTCGGC GGTGGGCGTC ACACATCTGG CTCTGCACAG ATGTGATGTT 
TACGGAGAGG CCTGTGCCGA CTGCTGCCTG GCCAGAGACC CATACTGTGC CTGGGACGGA 
AAGTCCTGCT CTCGCTACTC AGCAAACCAA AAAAGGCGGA GTCGAAGGCA AGACGTGAAG 
TATGGAAACC CTATAAGGCA ATGCAGAGGC TACAACTCGA ATGCCAATAA GAACACATTG 
GAGACAGTGC AGTATGGAGT AGAGGGAAGC ACAGCCTTTT TGGAGTGTCA GGCCAGATCT 
CCTCATGTGT CCATCAAGTG GCATTTCCAG AAGGAGAACA GCGACCGGAG AAGAGAGATC 
CGTTCTGACG GGCGTGTGGT TCGCACGGAA CAGGGCCTCC TCCTGCGCTC CCTCCAGCTG 
TCTGACAGCG GTGTTTACCA GTGCACCTCA ACTGAGAAGA ACTTCAAACA CACCCTTGTC 
AAACTCCAGC TCGTGGTTCT CTCCGCCCAC ACAGTCAACA GCATCCAGAC TGAAAGCGGC 
GCCCCGGCCG CCCCACCCCT TCAGTCCAGC GCCTGGACGC CCAGCGCAGA ACAATACAAA 
GACCTCCTGA CCATCCTCAG CCAACCTGAG ATGGGCCTCA TCAACCAGTA CTGCCAAGAC 
TACTGGCAGA TAGGGGACGA CCCCATGGCC CACAAGAAGA AGGACCTCAA GGAGCTCCAG 
AAGGACCTGC GGAAGCCTCG AAATAGGAGA CACCACCAGG AGCAGAGCAG CATGGCTGAG 
ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166096.1
CDS	531..2936
Translation	MDKVIMQGAGTLVLGTLLVSVFSAVSVHSSPLSSLPPASVPRVFLSFKELKSTGTAHHFAFLLNSTDYRILRMDEDHDRMYVGSKDYILSLDLNDINKEPLIIHWPVAPQKRTECVLSGKDINGECGNFIRLIEPWNRTHLYVCGTGAYNPICTYVDRGQRAQGFLQDQMPRAGGRTSRAADPSASPEPYAPKEYIFRLEPGKVDSGKGKCPYDPKLNSVSALINGQLYAGVYIDFMGTDSAIFRTLGKHTAMRTDQYNSRWLHDPTFVHAQLIPDSAEKNDDKLYFFFREKASEMGQTPMAQSRIGRICLNDDGGHCCLVNKWSTFLKARLICSVTGSDGIETHFDELRDVYIQKTQDTKNPVIYGVFSVSGSVFKGSAVCVYSMADVRMVFNGPFAHKEGPNYQWVAYQGKIPYPRPGTCPGGTFTPNMKSTKDYPDEVINFMRTHPTMFNAVYPVHKRPLVVRTNVDYEFTTITVDQVAAADGNYEVLFLGTDRGTVQKVIVLPRDDLQTEELVLEEVEVFRVPTSITTMKISPKRQQLYVSSAVGVTHLALHRCDVYGEACADCCLARDPYCAWDGKSCSRYSANQKRRSRRQDVKYGNPIRQCRGYNSNANKNTLETVQYGVEGSTAFLECQARSPHVSIKWHFQKENSDRRREIRSDGRVVRTEQGLLLRSLQLSDSGVYQCTSTEKNFKHTLVKLQLVVLSAHTVNSIQTESGAPAAPPLQSSAWTPSAEQYKDLLTILSQPEMGLINQYCQDYWQIGDDPMAHKKKDLKELQKDLRKPRNRRHHQEQSSMAET

Target ORF information:

RefSeq Version	XM_005166039.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fa (sema3fa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166039.3

ORF Insert Sequence:

ATGGATAAAG TGATCATGCA GGGAGCCGGG ACTTTGGTGC TGGGGACCCT TCTGGTCTCT 
GTATTCTCAG CTGTCTCGGT CCACAGCAGC CCTTTGTCTT CCCTGCCACC CGCCTCTGTT 
CCACGAGTCT TCCTGTCCTT CAAAGAGCTG AAGTCTACTG GAACAGCCCA TCACTTTGCC 
TTCCTGCTGA ACTCGACGGA CTACAGAATC CTCCGCATGG ACGAGGATCA TGATCGCATG 
TATGTGGGCA GCAAGGATTA CATTCTGTCT CTAGACCTGA ATGACATCAA CAAAGAGCCC 
CTTATAATTC ACTGGCCTGT AGCTCCACAG AAAAGGACTG AATGTGTACT CTCAGGGAAA 
GACATCAATG GCGAATGTGG TAATTTCATC CGTCTAATCG AGCCGTGGAA CCGGACTCAC 
CTGTATGTGT GCGGGACGGG AGCTTACAAC CCCATCTGCA CCTATGTGGA CAGAGGCCAA 
AGGGCACAGG GTTTTCTGCA AGATCAGATG CCTCGTGCAG GAGGCCGAAC CAGTCGAGCT 
GCTGACCCTA GTGCCTCACC AGAACCATAT GCACCCAAGG AATACATTTT CCGACTGGAG 
CCTGGTAAAG TAGATTCTGG GAAAGGAAAA TGCCCCTACG ACCCCAAACT CAACAGCGTC 
TCAGCTTTGA TAAATGGCCA GCTTTATGCC GGCGTCTACA TCGATTTTAT GGGAACAGAC 
TCTGCCATCT TTCGCACGTT GGGCAAACAC ACGGCAATGA GGACCGACCA GTATAACTCC 
CGCTGGCTCC ACGATCCAAC ATTTGTCCAT GCTCAGCTCA TCCCTGATAG TGCAGAAAAG 
AATGACGACA AGCTGTATTT CTTCTTCCGT GAGAAGGCCT CTGAGATGGG TCAGACTCCC 
ATGGCTCAAT CTAGGATTGG CAGAATTTGC CTGAATGATG ATGGAGGACA CTGCTGTCTG 
GTGAACAAGT GGAGCACCTT CCTGAAGGCT CGGCTCATCT GCTCTGTCAC CGGCTCTGAT 
GGCATCGAGA CGCATTTCGA TGAGCTCAGG GACGTGTACA TCCAGAAGAC TCAAGACACA 
AAGAATCCTG TCATCTACGG CGTCTTTTCC GTGTCCGGGT CTGTGTTTAA AGGCTCAGCA 
GTATGTGTTT ATTCAATGGC TGATGTGCGC ATGGTCTTCA ATGGGCCTTT CGCTCATAAA 
GAAGGCCCAA ACTACCAATG GGTTGCATAC CAGGGCAAAA TCCCATACCC TCGCCCAGGA 
ACATGTCCAG GAGGGACGTT TACCCCCAAC ATGAAGTCTA CTAAAGATTA CCCAGATGAG 
GTGATCAACT TCATGCGCAC ACATCCCACC ATGTTCAATG CAGTTTATCC AGTCCACAAG 
CGCCCCCTGG TGGTACGAAC CAACGTGGAT TATGAGTTCA CCACCATCAC TGTGGACCAA 
GTGGCTGCAG CTGATGGCAA CTATGAAGTT CTGTTCTTAG GAACAGACAG GGGAACAGTT 
CAGAAAGTGA TTGTCCTGCC CAGAGATGAT CTACAGACTG AAGAGCTGGT TTTGGAGGAG 
GTGGAGGTCT TCAGAGTTCC AACTTCAATC ACTACTATGA AGATTTCTCC AAAACGACAA 
CAGCTGTATG TCAGTTCGGC GGTGGGCGTC ACACATCTGG CTCTGCACAG ATGTGATGTT 
TACGGAGAGG CCTGTGCCGA CTGCTGCCTG GCCAGAGACC CATACTGTGC CTGGGACGGA 
AAGTCCTGCT CTCGCTACTC AGCAAACCAA AAAAGGCGGA GTCGAAGGCA AGACGTGAAG 
TATGGAAACC CTATAAGGCA ATGCAGAGGC TACAACTCGA ATGCCAATAA GAACACATTG 
GAGACAGTGC AGTATGGAGT AGAGGGAAGC ACAGCCTTTT TGGAGTGTCA GGCCAGATCT 
CCTCATGTGT CCATCAAGTG GCATTTCCAG AAGGAGAACA GCGACCGGAG AAGAGAGATC 
CGTTCTGACG GGCGTGTGGT TCGCACGGAA CAGGGCCTCC TCCTGCGCTC CCTCCAGCTG 
TCTGACAGCG GTGTTTACCA GTGCACCTCA ACTGAGAAGA ACTTCAAACA CACCCTTGTC 
AAACTCCAGC TCGTGGTTCT CTCCGCCCAC ACAGTCAACA GCATCCAGAC TGAAAGCGGC 
GCCCCGGCCG CCCCACCCCT TCAGTCCAGC GCCTGGACGC CCAGCGCAGA ACAATACAAA 
GACCTCCTGA CCATCCTCAG CCAACCTGAG ATGGGCCTCA TCAACCAGTA CTGCCAAGAC 
TACTGGCAGA TAGGGGACGA CCCCATGGCC CACAAGAAGA AGGACCTCAA GGAGCTCCAG 
AAGGACCTGC GGAAGCCTCG AAATAGGAGA CACCACCAGG AGCAGAGCAG CATGGCTGAG 
ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA3FA ( XM_005166039.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005166096.1 Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fa (sema3fa), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa12050
Clone ID Related Accession (Same CDS sequence)	XM_005166039.3 , XM_005166039.2
Accession Version	XM_005166039.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fa isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005166039.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAG TGATCATGCA GGGAGCCGGG ACTTTGGTGC TGGGGACCCT TCTGGTCTCT 
GTATTCTCAG CTGTCTCGGT CCACAGCAGC CCTTTGTCTT CCCTGCCACC CGCCTCTGTT 
CCACGAGTCT TCCTGTCCTT CAAAGAGCTG AAGTCTACTG GAACAGCCCA TCACTTTGCC 
TTCCTGCTGA ACTCGACGGA CTACAGAATC CTCCGCATGG ACGAGGATCA TGATCGCATG 
TATGTGGGCA GCAAGGATTA CATTCTGTCT CTAGACCTGA ATGACATCAA CAAAGAGCCC 
CTTATAATTC ACTGGCCTGT AGCTCCACAG AAAAGGACTG AATGTGTACT CTCAGGGAAA 
GACATCAATG GCGAATGTGG TAATTTCATC CGTCTAATCG AGCCGTGGAA CCGGACTCAC 
CTGTATGTGT GCGGGACGGG AGCTTACAAC CCCATCTGCA CCTATGTGGA CAGAGGCCAA 
AGGGCACAGG GTTTTCTGCA AGATCAGATG CCTCGTGCAG GAGGCCGAAC CAGTCGAGCT 
GCTGACCCTA GTGCCTCACC AGAACCATAT GCACCCAAGG AATACATTTT CCGACTGGAG 
CCTGGTAAAG TAGATTCTGG GAAAGGAAAA TGCCCCTACG ACCCCAAACT CAACAGCGTC 
TCAGCTTTGA TAAATGGCCA GCTTTATGCC GGCGTCTACA TCGATTTTAT GGGAACAGAC 
TCTGCCATCT TTCGCACGTT GGGCAAACAC ACGGCAATGA GGACCGACCA GTATAACTCC 
CGCTGGCTCC ACGATCCAAC ATTTGTCCAT GCTCAGCTCA TCCCTGATAG TGCAGAAAAG 
AATGACGACA AGCTGTATTT CTTCTTCCGT GAGAAGGCCT CTGAGATGGG TCAGACTCCC 
ATGGCTCAAT CTAGGATTGG CAGAATTTGC CTGAATGATG ATGGAGGACA CTGCTGTCTG 
GTGAACAAGT GGAGCACCTT CCTGAAGGCT CGGCTCATCT GCTCTGTCAC CGGCTCTGAT 
GGCATCGAGA CGCATTTCGA TGAGCTCAGG GACGTGTACA TCCAGAAGAC TCAAGACACA 
AAGAATCCTG TCATCTACGG CGTCTTTTCC GTGTCCGGGT CTGTGTTTAA AGGCTCAGCA 
GTATGTGTTT ATTCAATGGC TGATGTGCGC ATGGTCTTCA ATGGGCCTTT CGCTCATAAA 
GAAGGCCCAA ACTACCAATG GGTTGCATAC CAGGGCAAAA TCCCATACCC TCGCCCAGGA 
ACATGTCCAG GAGGGACGTT TACCCCCAAC ATGAAGTCTA CTAAAGATTA CCCAGATGAG 
GTGATCAACT TCATGCGCAC ACATCCCACC ATGTTCAATG CAGTTTATCC AGTCCACAAG 
CGCCCCCTGG TGGTACGAAC CAACGTGGAT TATGAGTTCA CCACCATCAC TGTGGACCAA 
GTGGCTGCAG CTGATGGCAA CTATGAAGTT CTGTTCTTAG GAACAGACAG GGGAACAGTT 
CAGAAAGTGA TTGTCCTGCC CAGAGATGAT CTACAGACTG AAGAGCTGGT TTTGGAGGAG 
GTGGAGGTCT TCAGAGTTCC AACTTCAATC ACTACTATGA AGATTTCTCC AAAACGACAA 
CAGCTGTATG TCAGTTCGGC GGTGGGCGTC ACACATCTGG CTCTGCACAG ATGTGATGTT 
TACGGAGAGG CCTGTGCCGA CTGCTGCCTG GCCAGAGACC CATACTGTGC CTGGGACGGA 
AAGTCCTGCT CTCGCTACTC AGCAAACCAA AAAAGGCGGA GTCGAAGGCA AGACGTGAAG 
TATGGAAACC CTATAAGGCA ATGCAGAGGC TACAACTCGA ATGCCAATAA GAACACATTG 
GAGACAGTGC AGTATGGAGT AGAGGGAAGC ACAGCCTTTT TGGAGTGTCA GGCCAGATCT 
CCTCATGTGT CCATCAAGTG GCATTTCCAG AAGGAGAACA GCGACCGGAG AAGAGAGATC 
CGTTCTGACG GGCGTGTGGT TCGCACGGAA CAGGGCCTCC TCCTGCGCTC CCTCCAGCTG 
TCTGACAGCG GTGTTTACCA GTGCACCTCA ACTGAGAAGA ACTTCAAACA CACCCTTGTC 
AAACTCCAGC TCGTGGTTCT CTCCGCCCAC ACAGTCAACA GCATCCAGAC TGAAAGCGGC 
GCCCCGGCCG CCCCACCCCT TCAGTCCAGC GCCTGGACGC CCAGCGCAGA ACAATACAAA 
GACCTCCTGA CCATCCTCAG CCAACCTGAG ATGGGCCTCA TCAACCAGTA CTGCCAAGAC 
TACTGGCAGA TAGGGGACGA CCCCATGGCC CACAAGAAGA AGGACCTCAA GGAGCTCCAG 
AAGGACCTGC GGAAGCCTCG AAATAGGAGA CACCACCAGG AGCAGAGCAG CATGGCTGAG 
ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166096.1
CDS	501..2906
Translation	MDKVIMQGAGTLVLGTLLVSVFSAVSVHSSPLSSLPPASVPRVFLSFKELKSTGTAHHFAFLLNSTDYRILRMDEDHDRMYVGSKDYILSLDLNDINKEPLIIHWPVAPQKRTECVLSGKDINGECGNFIRLIEPWNRTHLYVCGTGAYNPICTYVDRGQRAQGFLQDQMPRAGGRTSRAADPSASPEPYAPKEYIFRLEPGKVDSGKGKCPYDPKLNSVSALINGQLYAGVYIDFMGTDSAIFRTLGKHTAMRTDQYNSRWLHDPTFVHAQLIPDSAEKNDDKLYFFFREKASEMGQTPMAQSRIGRICLNDDGGHCCLVNKWSTFLKARLICSVTGSDGIETHFDELRDVYIQKTQDTKNPVIYGVFSVSGSVFKGSAVCVYSMADVRMVFNGPFAHKEGPNYQWVAYQGKIPYPRPGTCPGGTFTPNMKSTKDYPDEVINFMRTHPTMFNAVYPVHKRPLVVRTNVDYEFTTITVDQVAAADGNYEVLFLGTDRGTVQKVIVLPRDDLQTEELVLEEVEVFRVPTSITTMKISPKRQQLYVSSAVGVTHLALHRCDVYGEACADCCLARDPYCAWDGKSCSRYSANQKRRSRRQDVKYGNPIRQCRGYNSNANKNTLETVQYGVEGSTAFLECQARSPHVSIKWHFQKENSDRRREIRSDGRVVRTEQGLLLRSLQLSDSGVYQCTSTEKNFKHTLVKLQLVVLSAHTVNSIQTESGAPAAPPLQSSAWTPSAEQYKDLLTILSQPEMGLINQYCQDYWQIGDDPMAHKKKDLKELQKDLRKPRNRRHHQEQSSMAET

Target ORF information:

RefSeq Version	XM_005166039.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fa (sema3fa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166039.2

ORF Insert Sequence:

ATGGATAAAG TGATCATGCA GGGAGCCGGG ACTTTGGTGC TGGGGACCCT TCTGGTCTCT 
GTATTCTCAG CTGTCTCGGT CCACAGCAGC CCTTTGTCTT CCCTGCCACC CGCCTCTGTT 
CCACGAGTCT TCCTGTCCTT CAAAGAGCTG AAGTCTACTG GAACAGCCCA TCACTTTGCC 
TTCCTGCTGA ACTCGACGGA CTACAGAATC CTCCGCATGG ACGAGGATCA TGATCGCATG 
TATGTGGGCA GCAAGGATTA CATTCTGTCT CTAGACCTGA ATGACATCAA CAAAGAGCCC 
CTTATAATTC ACTGGCCTGT AGCTCCACAG AAAAGGACTG AATGTGTACT CTCAGGGAAA 
GACATCAATG GCGAATGTGG TAATTTCATC CGTCTAATCG AGCCGTGGAA CCGGACTCAC 
CTGTATGTGT GCGGGACGGG AGCTTACAAC CCCATCTGCA CCTATGTGGA CAGAGGCCAA 
AGGGCACAGG GTTTTCTGCA AGATCAGATG CCTCGTGCAG GAGGCCGAAC CAGTCGAGCT 
GCTGACCCTA GTGCCTCACC AGAACCATAT GCACCCAAGG AATACATTTT CCGACTGGAG 
CCTGGTAAAG TAGATTCTGG GAAAGGAAAA TGCCCCTACG ACCCCAAACT CAACAGCGTC 
TCAGCTTTGA TAAATGGCCA GCTTTATGCC GGCGTCTACA TCGATTTTAT GGGAACAGAC 
TCTGCCATCT TTCGCACGTT GGGCAAACAC ACGGCAATGA GGACCGACCA GTATAACTCC 
CGCTGGCTCC ACGATCCAAC ATTTGTCCAT GCTCAGCTCA TCCCTGATAG TGCAGAAAAG 
AATGACGACA AGCTGTATTT CTTCTTCCGT GAGAAGGCCT CTGAGATGGG TCAGACTCCC 
ATGGCTCAAT CTAGGATTGG CAGAATTTGC CTGAATGATG ATGGAGGACA CTGCTGTCTG 
GTGAACAAGT GGAGCACCTT CCTGAAGGCT CGGCTCATCT GCTCTGTCAC CGGCTCTGAT 
GGCATCGAGA CGCATTTCGA TGAGCTCAGG GACGTGTACA TCCAGAAGAC TCAAGACACA 
AAGAATCCTG TCATCTACGG CGTCTTTTCC GTGTCCGGGT CTGTGTTTAA AGGCTCAGCA 
GTATGTGTTT ATTCAATGGC TGATGTGCGC ATGGTCTTCA ATGGGCCTTT CGCTCATAAA 
GAAGGCCCAA ACTACCAATG GGTTGCATAC CAGGGCAAAA TCCCATACCC TCGCCCAGGA 
ACATGTCCAG GAGGGACGTT TACCCCCAAC ATGAAGTCTA CTAAAGATTA CCCAGATGAG 
GTGATCAACT TCATGCGCAC ACATCCCACC ATGTTCAATG CAGTTTATCC AGTCCACAAG 
CGCCCCCTGG TGGTACGAAC CAACGTGGAT TATGAGTTCA CCACCATCAC TGTGGACCAA 
GTGGCTGCAG CTGATGGCAA CTATGAAGTT CTGTTCTTAG GAACAGACAG GGGAACAGTT 
CAGAAAGTGA TTGTCCTGCC CAGAGATGAT CTACAGACTG AAGAGCTGGT TTTGGAGGAG 
GTGGAGGTCT TCAGAGTTCC AACTTCAATC ACTACTATGA AGATTTCTCC AAAACGACAA 
CAGCTGTATG TCAGTTCGGC GGTGGGCGTC ACACATCTGG CTCTGCACAG ATGTGATGTT 
TACGGAGAGG CCTGTGCCGA CTGCTGCCTG GCCAGAGACC CATACTGTGC CTGGGACGGA 
AAGTCCTGCT CTCGCTACTC AGCAAACCAA AAAAGGCGGA GTCGAAGGCA AGACGTGAAG 
TATGGAAACC CTATAAGGCA ATGCAGAGGC TACAACTCGA ATGCCAATAA GAACACATTG 
GAGACAGTGC AGTATGGAGT AGAGGGAAGC ACAGCCTTTT TGGAGTGTCA GGCCAGATCT 
CCTCATGTGT CCATCAAGTG GCATTTCCAG AAGGAGAACA GCGACCGGAG AAGAGAGATC 
CGTTCTGACG GGCGTGTGGT TCGCACGGAA CAGGGCCTCC TCCTGCGCTC CCTCCAGCTG 
TCTGACAGCG GTGTTTACCA GTGCACCTCA ACTGAGAAGA ACTTCAAACA CACCCTTGTC 
AAACTCCAGC TCGTGGTTCT CTCCGCCCAC ACAGTCAACA GCATCCAGAC TGAAAGCGGC 
GCCCCGGCCG CCCCACCCCT TCAGTCCAGC GCCTGGACGC CCAGCGCAGA ACAATACAAA 
GACCTCCTGA CCATCCTCAG CCAACCTGAG ATGGGCCTCA TCAACCAGTA CTGCCAAGAC 
TACTGGCAGA TAGGGGACGA CCCCATGGCC CACAAGAAGA AGGACCTCAA GGAGCTCCAG 
AAGGACCTGC GGAAGCCTCG AAATAGGAGA CACCACCAGG AGCAGAGCAG CATGGCTGAG 
ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA3FA ( XM_005166040.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005166097.1 Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fa (sema3fa), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa12051
Clone ID Related Accession (Same CDS sequence)	XM_005166040.2 , XM_005166040.3
Accession Version	XM_005166040.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fa isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005166040.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAG TGATCATGCA GGGAGCCGGG ACTTTGGTGC TGGGGACCCT TCTGGTCTCT 
GTATTCTCAG CTGTCTCGGT CCACAGCAGC CCTTTGTCTT CCCTGCCACC CGCCTCTGTT 
CCACGAGTCT TCCTGTCCTT CAAAGAGCTG AAGTCTACTG GAACAGCCCA TCACTTTGCC 
TTCCTGCTGA ACTCGACGGA CTACAGAATC CTCCGCATGG ACGAGGATCA TGATCGCATG 
TATGTGGGCA GCAAGGATTA CATTCTGTCT CTAGACCTGA ATGACATCAA CAAAGAGCCC 
CTTATAATTC ACTGGCCTGT AGCTCCACAG AAAAGGACTG AATGTGTACT CTCAGGGAAA 
GACATCAATG GCGAATGTGG TAATTTCATC CGTCTAATCG AGCCGTGGAA CCGGACTCAC 
CTGTATGTGT GCGGGACGGG AGCTTACAAC CCCATCTGCA CCTATGTGGA CAGAGGCCAA 
AGGGCACAGG AATACATTTT CCGACTGGAG CCTGGTAAAG TAGATTCTGG GAAAGGAAAA 
TGCCCCTACG ACCCCAAACT CAACAGCGTC TCAGCTTTGA TAAATGGCCA GCTTTATGCC 
GGCGTCTACA TCGATTTTAT GGGAACAGAC TCTGCCATCT TTCGCACGTT GGGCAAACAC 
ACGGCAATGA GGACCGACCA GTATAACTCC CGCTGGCTCC ACGATCCAAC ATTTGTCCAT 
GCTCAGCTCA TCCCTGATAG TGCAGAAAAG AATGACGACA AGCTGTATTT CTTCTTCCGT 
GAGAAGGCCT CTGAGATGGG TCAGACTCCC ATGGCTCAAT CTAGGATTGG CAGAATTTGC 
CTGAATGATG ATGGAGGACA CTGCTGTCTG GTGAACAAGT GGAGCACCTT CCTGAAGGCT 
CGGCTCATCT GCTCTGTCAC CGGCTCTGAT GGCATCGAGA CGCATTTCGA TGAGCTCAGG 
GACGTGTACA TCCAGAAGAC TCAAGACACA AAGAATCCTG TCATCTACGG CGTCTTTTCC 
GTGTCCGGGT CTGTGTTTAA AGGCTCAGCA GTATGTGTTT ATTCAATGGC TGATGTGCGC 
ATGGTCTTCA ATGGGCCTTT CGCTCATAAA GAAGGCCCAA ACTACCAATG GGTTGCATAC 
CAGGGCAAAA TCCCATACCC TCGCCCAGGA ACATGTCCAG GAGGGACGTT TACCCCCAAC 
ATGAAGTCTA CTAAAGATTA CCCAGATGAG GTGATCAACT TCATGCGCAC ACATCCCACC 
ATGTTCAATG CAGTTTATCC AGTCCACAAG CGCCCCCTGG TGGTACGAAC CAACGTGGAT 
TATGAGTTCA CCACCATCAC TGTGGACCAA GTGGCTGCAG CTGATGGCAA CTATGAAGTT 
CTGTTCTTAG GAACAGACAG GGGAACAGTT CAGAAAGTGA TTGTCCTGCC CAGAGATGAT 
CTACAGACTG AAGAGCTGGT TTTGGAGGAG GTGGAGGTCT TCAGAGTTCC AACTTCAATC 
ACTACTATGA AGATTTCTCC AAAACGACAA CAGCTGTATG TCAGTTCGGC GGTGGGCGTC 
ACACATCTGG CTCTGCACAG ATGTGATGTT TACGGAGAGG CCTGTGCCGA CTGCTGCCTG 
GCCAGAGACC CATACTGTGC CTGGGACGGA AAGTCCTGCT CTCGCTACTC AGCAAACCAA 
AAAAGGCGGA GTCGAAGGCA AGACGTGAAG TATGGAAACC CTATAAGGCA ATGCAGAGGC 
TACAACTCGA ATGCCAATAA GAACACATTG GAGACAGTGC AGTATGGAGT AGAGGGAAGC 
ACAGCCTTTT TGGAGTGTCA GGCCAGATCT CCTCATGTGT CCATCAAGTG GCATTTCCAG 
AAGGAGAACA GCGACCGGAG AAGAGAGATC CGTTCTGACG GGCGTGTGGT TCGCACGGAA 
CAGGGCCTCC TCCTGCGCTC CCTCCAGCTG TCTGACAGCG GTGTTTACCA GTGCACCTCA 
ACTGAGAAGA ACTTCAAACA CACCCTTGTC AAACTCCAGC TCGTGGTTCT CTCCGCCCAC 
ACAGTCAACA GCATCCAGAC TGAAAGCGGC GCCCCGGCCG CCCCACCCCT TCAGTCCAGC 
GCCTGGACGC CCAGCGCAGA ACAATACAAA GACCTCCTGA CCATCCTCAG CCAACCTGAG 
ATGGGCCTCA TCAACCAGTA CTGCCAAGAC TACTGGCAGA TAGGGGACGA CCCCATGGCC 
CACAAGAAGA AGGACCTCAA GGAGCTCCAG AAGGACCTGC GGAAGCCTCG AAATAGGAGA 
CACCACCAGG AGCAGAGCAG CATGGCTGAG ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166097.1
CDS	501..2816
Translation	MDKVIMQGAGTLVLGTLLVSVFSAVSVHSSPLSSLPPASVPRVFLSFKELKSTGTAHHFAFLLNSTDYRILRMDEDHDRMYVGSKDYILSLDLNDINKEPLIIHWPVAPQKRTECVLSGKDINGECGNFIRLIEPWNRTHLYVCGTGAYNPICTYVDRGQRAQEYIFRLEPGKVDSGKGKCPYDPKLNSVSALINGQLYAGVYIDFMGTDSAIFRTLGKHTAMRTDQYNSRWLHDPTFVHAQLIPDSAEKNDDKLYFFFREKASEMGQTPMAQSRIGRICLNDDGGHCCLVNKWSTFLKARLICSVTGSDGIETHFDELRDVYIQKTQDTKNPVIYGVFSVSGSVFKGSAVCVYSMADVRMVFNGPFAHKEGPNYQWVAYQGKIPYPRPGTCPGGTFTPNMKSTKDYPDEVINFMRTHPTMFNAVYPVHKRPLVVRTNVDYEFTTITVDQVAAADGNYEVLFLGTDRGTVQKVIVLPRDDLQTEELVLEEVEVFRVPTSITTMKISPKRQQLYVSSAVGVTHLALHRCDVYGEACADCCLARDPYCAWDGKSCSRYSANQKRRSRRQDVKYGNPIRQCRGYNSNANKNTLETVQYGVEGSTAFLECQARSPHVSIKWHFQKENSDRRREIRSDGRVVRTEQGLLLRSLQLSDSGVYQCTSTEKNFKHTLVKLQLVVLSAHTVNSIQTESGAPAAPPLQSSAWTPSAEQYKDLLTILSQPEMGLINQYCQDYWQIGDDPMAHKKKDLKELQKDLRKPRNRRHHQEQSSMAET

Target ORF information:

RefSeq Version	XM_005166040.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fa (sema3fa), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166040.2

ORF Insert Sequence:

ATGGATAAAG TGATCATGCA GGGAGCCGGG ACTTTGGTGC TGGGGACCCT TCTGGTCTCT 
GTATTCTCAG CTGTCTCGGT CCACAGCAGC CCTTTGTCTT CCCTGCCACC CGCCTCTGTT 
CCACGAGTCT TCCTGTCCTT CAAAGAGCTG AAGTCTACTG GAACAGCCCA TCACTTTGCC 
TTCCTGCTGA ACTCGACGGA CTACAGAATC CTCCGCATGG ACGAGGATCA TGATCGCATG 
TATGTGGGCA GCAAGGATTA CATTCTGTCT CTAGACCTGA ATGACATCAA CAAAGAGCCC 
CTTATAATTC ACTGGCCTGT AGCTCCACAG AAAAGGACTG AATGTGTACT CTCAGGGAAA 
GACATCAATG GCGAATGTGG TAATTTCATC CGTCTAATCG AGCCGTGGAA CCGGACTCAC 
CTGTATGTGT GCGGGACGGG AGCTTACAAC CCCATCTGCA CCTATGTGGA CAGAGGCCAA 
AGGGCACAGG AATACATTTT CCGACTGGAG CCTGGTAAAG TAGATTCTGG GAAAGGAAAA 
TGCCCCTACG ACCCCAAACT CAACAGCGTC TCAGCTTTGA TAAATGGCCA GCTTTATGCC 
GGCGTCTACA TCGATTTTAT GGGAACAGAC TCTGCCATCT TTCGCACGTT GGGCAAACAC 
ACGGCAATGA GGACCGACCA GTATAACTCC CGCTGGCTCC ACGATCCAAC ATTTGTCCAT 
GCTCAGCTCA TCCCTGATAG TGCAGAAAAG AATGACGACA AGCTGTATTT CTTCTTCCGT 
GAGAAGGCCT CTGAGATGGG TCAGACTCCC ATGGCTCAAT CTAGGATTGG CAGAATTTGC 
CTGAATGATG ATGGAGGACA CTGCTGTCTG GTGAACAAGT GGAGCACCTT CCTGAAGGCT 
CGGCTCATCT GCTCTGTCAC CGGCTCTGAT GGCATCGAGA CGCATTTCGA TGAGCTCAGG 
GACGTGTACA TCCAGAAGAC TCAAGACACA AAGAATCCTG TCATCTACGG CGTCTTTTCC 
GTGTCCGGGT CTGTGTTTAA AGGCTCAGCA GTATGTGTTT ATTCAATGGC TGATGTGCGC 
ATGGTCTTCA ATGGGCCTTT CGCTCATAAA GAAGGCCCAA ACTACCAATG GGTTGCATAC 
CAGGGCAAAA TCCCATACCC TCGCCCAGGA ACATGTCCAG GAGGGACGTT TACCCCCAAC 
ATGAAGTCTA CTAAAGATTA CCCAGATGAG GTGATCAACT TCATGCGCAC ACATCCCACC 
ATGTTCAATG CAGTTTATCC AGTCCACAAG CGCCCCCTGG TGGTACGAAC CAACGTGGAT 
TATGAGTTCA CCACCATCAC TGTGGACCAA GTGGCTGCAG CTGATGGCAA CTATGAAGTT 
CTGTTCTTAG GAACAGACAG GGGAACAGTT CAGAAAGTGA TTGTCCTGCC CAGAGATGAT 
CTACAGACTG AAGAGCTGGT TTTGGAGGAG GTGGAGGTCT TCAGAGTTCC AACTTCAATC 
ACTACTATGA AGATTTCTCC AAAACGACAA CAGCTGTATG TCAGTTCGGC GGTGGGCGTC 
ACACATCTGG CTCTGCACAG ATGTGATGTT TACGGAGAGG CCTGTGCCGA CTGCTGCCTG 
GCCAGAGACC CATACTGTGC CTGGGACGGA AAGTCCTGCT CTCGCTACTC AGCAAACCAA 
AAAAGGCGGA GTCGAAGGCA AGACGTGAAG TATGGAAACC CTATAAGGCA ATGCAGAGGC 
TACAACTCGA ATGCCAATAA GAACACATTG GAGACAGTGC AGTATGGAGT AGAGGGAAGC 
ACAGCCTTTT TGGAGTGTCA GGCCAGATCT CCTCATGTGT CCATCAAGTG GCATTTCCAG 
AAGGAGAACA GCGACCGGAG AAGAGAGATC CGTTCTGACG GGCGTGTGGT TCGCACGGAA 
CAGGGCCTCC TCCTGCGCTC CCTCCAGCTG TCTGACAGCG GTGTTTACCA GTGCACCTCA 
ACTGAGAAGA ACTTCAAACA CACCCTTGTC AAACTCCAGC TCGTGGTTCT CTCCGCCCAC 
ACAGTCAACA GCATCCAGAC TGAAAGCGGC GCCCCGGCCG CCCCACCCCT TCAGTCCAGC 
GCCTGGACGC CCAGCGCAGA ACAATACAAA GACCTCCTGA CCATCCTCAG CCAACCTGAG 
ATGGGCCTCA TCAACCAGTA CTGCCAAGAC TACTGGCAGA TAGGGGACGA CCCCATGGCC 
CACAAGAAGA AGGACCTCAA GGAGCTCCAG AAGGACCTGC GGAAGCCTCG AAATAGGAGA 
CACCACCAGG AGCAGAGCAG CATGGCTGAG ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA3FA ( XM_005166040.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005166097.1 Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fa (sema3fa), transcript variant X2, mRNA.

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CloneID	ODa12051
Clone ID Related Accession (Same CDS sequence)	XM_005166040.2 , XM_005166040.3
Accession Version	XM_005166040.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fa isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005166040.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAG TGATCATGCA GGGAGCCGGG ACTTTGGTGC TGGGGACCCT TCTGGTCTCT 
GTATTCTCAG CTGTCTCGGT CCACAGCAGC CCTTTGTCTT CCCTGCCACC CGCCTCTGTT 
CCACGAGTCT TCCTGTCCTT CAAAGAGCTG AAGTCTACTG GAACAGCCCA TCACTTTGCC 
TTCCTGCTGA ACTCGACGGA CTACAGAATC CTCCGCATGG ACGAGGATCA TGATCGCATG 
TATGTGGGCA GCAAGGATTA CATTCTGTCT CTAGACCTGA ATGACATCAA CAAAGAGCCC 
CTTATAATTC ACTGGCCTGT AGCTCCACAG AAAAGGACTG AATGTGTACT CTCAGGGAAA 
GACATCAATG GCGAATGTGG TAATTTCATC CGTCTAATCG AGCCGTGGAA CCGGACTCAC 
CTGTATGTGT GCGGGACGGG AGCTTACAAC CCCATCTGCA CCTATGTGGA CAGAGGCCAA 
AGGGCACAGG AATACATTTT CCGACTGGAG CCTGGTAAAG TAGATTCTGG GAAAGGAAAA 
TGCCCCTACG ACCCCAAACT CAACAGCGTC TCAGCTTTGA TAAATGGCCA GCTTTATGCC 
GGCGTCTACA TCGATTTTAT GGGAACAGAC TCTGCCATCT TTCGCACGTT GGGCAAACAC 
ACGGCAATGA GGACCGACCA GTATAACTCC CGCTGGCTCC ACGATCCAAC ATTTGTCCAT 
GCTCAGCTCA TCCCTGATAG TGCAGAAAAG AATGACGACA AGCTGTATTT CTTCTTCCGT 
GAGAAGGCCT CTGAGATGGG TCAGACTCCC ATGGCTCAAT CTAGGATTGG CAGAATTTGC 
CTGAATGATG ATGGAGGACA CTGCTGTCTG GTGAACAAGT GGAGCACCTT CCTGAAGGCT 
CGGCTCATCT GCTCTGTCAC CGGCTCTGAT GGCATCGAGA CGCATTTCGA TGAGCTCAGG 
GACGTGTACA TCCAGAAGAC TCAAGACACA AAGAATCCTG TCATCTACGG CGTCTTTTCC 
GTGTCCGGGT CTGTGTTTAA AGGCTCAGCA GTATGTGTTT ATTCAATGGC TGATGTGCGC 
ATGGTCTTCA ATGGGCCTTT CGCTCATAAA GAAGGCCCAA ACTACCAATG GGTTGCATAC 
CAGGGCAAAA TCCCATACCC TCGCCCAGGA ACATGTCCAG GAGGGACGTT TACCCCCAAC 
ATGAAGTCTA CTAAAGATTA CCCAGATGAG GTGATCAACT TCATGCGCAC ACATCCCACC 
ATGTTCAATG CAGTTTATCC AGTCCACAAG CGCCCCCTGG TGGTACGAAC CAACGTGGAT 
TATGAGTTCA CCACCATCAC TGTGGACCAA GTGGCTGCAG CTGATGGCAA CTATGAAGTT 
CTGTTCTTAG GAACAGACAG GGGAACAGTT CAGAAAGTGA TTGTCCTGCC CAGAGATGAT 
CTACAGACTG AAGAGCTGGT TTTGGAGGAG GTGGAGGTCT TCAGAGTTCC AACTTCAATC 
ACTACTATGA AGATTTCTCC AAAACGACAA CAGCTGTATG TCAGTTCGGC GGTGGGCGTC 
ACACATCTGG CTCTGCACAG ATGTGATGTT TACGGAGAGG CCTGTGCCGA CTGCTGCCTG 
GCCAGAGACC CATACTGTGC CTGGGACGGA AAGTCCTGCT CTCGCTACTC AGCAAACCAA 
AAAAGGCGGA GTCGAAGGCA AGACGTGAAG TATGGAAACC CTATAAGGCA ATGCAGAGGC 
TACAACTCGA ATGCCAATAA GAACACATTG GAGACAGTGC AGTATGGAGT AGAGGGAAGC 
ACAGCCTTTT TGGAGTGTCA GGCCAGATCT CCTCATGTGT CCATCAAGTG GCATTTCCAG 
AAGGAGAACA GCGACCGGAG AAGAGAGATC CGTTCTGACG GGCGTGTGGT TCGCACGGAA 
CAGGGCCTCC TCCTGCGCTC CCTCCAGCTG TCTGACAGCG GTGTTTACCA GTGCACCTCA 
ACTGAGAAGA ACTTCAAACA CACCCTTGTC AAACTCCAGC TCGTGGTTCT CTCCGCCCAC 
ACAGTCAACA GCATCCAGAC TGAAAGCGGC GCCCCGGCCG CCCCACCCCT TCAGTCCAGC 
GCCTGGACGC CCAGCGCAGA ACAATACAAA GACCTCCTGA CCATCCTCAG CCAACCTGAG 
ATGGGCCTCA TCAACCAGTA CTGCCAAGAC TACTGGCAGA TAGGGGACGA CCCCATGGCC 
CACAAGAAGA AGGACCTCAA GGAGCTCCAG AAGGACCTGC GGAAGCCTCG AAATAGGAGA 
CACCACCAGG AGCAGAGCAG CATGGCTGAG ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166097.1
CDS	531..2846
Translation	MDKVIMQGAGTLVLGTLLVSVFSAVSVHSSPLSSLPPASVPRVFLSFKELKSTGTAHHFAFLLNSTDYRILRMDEDHDRMYVGSKDYILSLDLNDINKEPLIIHWPVAPQKRTECVLSGKDINGECGNFIRLIEPWNRTHLYVCGTGAYNPICTYVDRGQRAQEYIFRLEPGKVDSGKGKCPYDPKLNSVSALINGQLYAGVYIDFMGTDSAIFRTLGKHTAMRTDQYNSRWLHDPTFVHAQLIPDSAEKNDDKLYFFFREKASEMGQTPMAQSRIGRICLNDDGGHCCLVNKWSTFLKARLICSVTGSDGIETHFDELRDVYIQKTQDTKNPVIYGVFSVSGSVFKGSAVCVYSMADVRMVFNGPFAHKEGPNYQWVAYQGKIPYPRPGTCPGGTFTPNMKSTKDYPDEVINFMRTHPTMFNAVYPVHKRPLVVRTNVDYEFTTITVDQVAAADGNYEVLFLGTDRGTVQKVIVLPRDDLQTEELVLEEVEVFRVPTSITTMKISPKRQQLYVSSAVGVTHLALHRCDVYGEACADCCLARDPYCAWDGKSCSRYSANQKRRSRRQDVKYGNPIRQCRGYNSNANKNTLETVQYGVEGSTAFLECQARSPHVSIKWHFQKENSDRRREIRSDGRVVRTEQGLLLRSLQLSDSGVYQCTSTEKNFKHTLVKLQLVVLSAHTVNSIQTESGAPAAPPLQSSAWTPSAEQYKDLLTILSQPEMGLINQYCQDYWQIGDDPMAHKKKDLKELQKDLRKPRNRRHHQEQSSMAET

Target ORF information:

RefSeq Version	XM_005166040.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fa (sema3fa), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166040.3

ORF Insert Sequence:

ATGGATAAAG TGATCATGCA GGGAGCCGGG ACTTTGGTGC TGGGGACCCT TCTGGTCTCT 
GTATTCTCAG CTGTCTCGGT CCACAGCAGC CCTTTGTCTT CCCTGCCACC CGCCTCTGTT 
CCACGAGTCT TCCTGTCCTT CAAAGAGCTG AAGTCTACTG GAACAGCCCA TCACTTTGCC 
TTCCTGCTGA ACTCGACGGA CTACAGAATC CTCCGCATGG ACGAGGATCA TGATCGCATG 
TATGTGGGCA GCAAGGATTA CATTCTGTCT CTAGACCTGA ATGACATCAA CAAAGAGCCC 
CTTATAATTC ACTGGCCTGT AGCTCCACAG AAAAGGACTG AATGTGTACT CTCAGGGAAA 
GACATCAATG GCGAATGTGG TAATTTCATC CGTCTAATCG AGCCGTGGAA CCGGACTCAC 
CTGTATGTGT GCGGGACGGG AGCTTACAAC CCCATCTGCA CCTATGTGGA CAGAGGCCAA 
AGGGCACAGG AATACATTTT CCGACTGGAG CCTGGTAAAG TAGATTCTGG GAAAGGAAAA 
TGCCCCTACG ACCCCAAACT CAACAGCGTC TCAGCTTTGA TAAATGGCCA GCTTTATGCC 
GGCGTCTACA TCGATTTTAT GGGAACAGAC TCTGCCATCT TTCGCACGTT GGGCAAACAC 
ACGGCAATGA GGACCGACCA GTATAACTCC CGCTGGCTCC ACGATCCAAC ATTTGTCCAT 
GCTCAGCTCA TCCCTGATAG TGCAGAAAAG AATGACGACA AGCTGTATTT CTTCTTCCGT 
GAGAAGGCCT CTGAGATGGG TCAGACTCCC ATGGCTCAAT CTAGGATTGG CAGAATTTGC 
CTGAATGATG ATGGAGGACA CTGCTGTCTG GTGAACAAGT GGAGCACCTT CCTGAAGGCT 
CGGCTCATCT GCTCTGTCAC CGGCTCTGAT GGCATCGAGA CGCATTTCGA TGAGCTCAGG 
GACGTGTACA TCCAGAAGAC TCAAGACACA AAGAATCCTG TCATCTACGG CGTCTTTTCC 
GTGTCCGGGT CTGTGTTTAA AGGCTCAGCA GTATGTGTTT ATTCAATGGC TGATGTGCGC 
ATGGTCTTCA ATGGGCCTTT CGCTCATAAA GAAGGCCCAA ACTACCAATG GGTTGCATAC 
CAGGGCAAAA TCCCATACCC TCGCCCAGGA ACATGTCCAG GAGGGACGTT TACCCCCAAC 
ATGAAGTCTA CTAAAGATTA CCCAGATGAG GTGATCAACT TCATGCGCAC ACATCCCACC 
ATGTTCAATG CAGTTTATCC AGTCCACAAG CGCCCCCTGG TGGTACGAAC CAACGTGGAT 
TATGAGTTCA CCACCATCAC TGTGGACCAA GTGGCTGCAG CTGATGGCAA CTATGAAGTT 
CTGTTCTTAG GAACAGACAG GGGAACAGTT CAGAAAGTGA TTGTCCTGCC CAGAGATGAT 
CTACAGACTG AAGAGCTGGT TTTGGAGGAG GTGGAGGTCT TCAGAGTTCC AACTTCAATC 
ACTACTATGA AGATTTCTCC AAAACGACAA CAGCTGTATG TCAGTTCGGC GGTGGGCGTC 
ACACATCTGG CTCTGCACAG ATGTGATGTT TACGGAGAGG CCTGTGCCGA CTGCTGCCTG 
GCCAGAGACC CATACTGTGC CTGGGACGGA AAGTCCTGCT CTCGCTACTC AGCAAACCAA 
AAAAGGCGGA GTCGAAGGCA AGACGTGAAG TATGGAAACC CTATAAGGCA ATGCAGAGGC 
TACAACTCGA ATGCCAATAA GAACACATTG GAGACAGTGC AGTATGGAGT AGAGGGAAGC 
ACAGCCTTTT TGGAGTGTCA GGCCAGATCT CCTCATGTGT CCATCAAGTG GCATTTCCAG 
AAGGAGAACA GCGACCGGAG AAGAGAGATC CGTTCTGACG GGCGTGTGGT TCGCACGGAA 
CAGGGCCTCC TCCTGCGCTC CCTCCAGCTG TCTGACAGCG GTGTTTACCA GTGCACCTCA 
ACTGAGAAGA ACTTCAAACA CACCCTTGTC AAACTCCAGC TCGTGGTTCT CTCCGCCCAC 
ACAGTCAACA GCATCCAGAC TGAAAGCGGC GCCCCGGCCG CCCCACCCCT TCAGTCCAGC 
GCCTGGACGC CCAGCGCAGA ACAATACAAA GACCTCCTGA CCATCCTCAG CCAACCTGAG 
ATGGGCCTCA TCAACCAGTA CTGCCAAGAC TACTGGCAGA TAGGGGACGA CCCCATGGCC 
CACAAGAAGA AGGACCTCAA GGAGCTCCAG AAGGACCTGC GGAAGCCTCG AAATAGGAGA 
CACCACCAGG AGCAGAGCAG CATGGCTGAG ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Semaphorin/neuropilin binding specificities are stable over 400 million years of evolution.
Biochemical and biophysical research communications517(1)23-28(2019 Sep)
He Z,Crenshaw E,Raper JA

Human Semaphorin 3 Variants Link Melanocortin Circuit Development and Energy Balance.
Cell176(4)729-742.e18(2019 02)
van der Klaauw AA,Croizier S,Mendes de Oliveira E,Stadler LKJ,Park S,Kong Y,Banton MC,Tandon P,Hendricks AE,Keogh JM,Riley SE,Papadia S,Henning E,Bounds R,Bochukova EG,Mistry V,O'Rahilly S,Simerly RB,,,Minchin JEN,Barroso I,Jones EY,Bouret SG,Farooqi IS

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Attractant and repellent cues cooperate in guiding a subset of olfactory sensory axons to a well-defined protoglomerular target.
Development (Cambridge, England)143(1)123-32(2016 Jan)
Taku AA,Marcaccio CL,Ye W,Krause GJ,Raper JA

Methyl-CpG Binding Protein 2 (Mecp2) Regulates Sensory Function Through Sema5b and Robo2.
Frontiers in cellular neuroscience9481(2015)
Leong WY,Lim ZH,Korzh V,Pietri T,Goh EL

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SEMA3FA cDNA ORF clone, Danio rerio(Zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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