nbn cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	nbn
Entrez Gene ID	544655
Full Name	nibrin
Synonyms	Nbs1,im:6911679,zgc:194152
General protein information	Preferred Names nibrin Names nibrin nijmegen breakage syndrome protein 1 homolog
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001014819.1	NP_001014819.1	nibrin

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	nbn	NP_001014819.1
Mus musculus (house mouse)	Nbn	NP_038780.3
Xenopus tropicalis (tropical clawed frog)	nbn	XP_004915210.1
Homo sapiens (human)	NBN	NP_002476.2
Pan troglodytes (chimpanzee)	NBN	XP_519851.3
Canis lupus familiaris (dog)	NBN	XP_005638161.1
Bos taurus (cattle)	NBN	NP_001069305.2
Gallus gallus (chicken)	NBN	NP_989668.1
Macaca mulatta (Rhesus monkey)	NBN	XP_001085033.1
Rattus norvegicus (Norway rat)	Nbn	NP_620228.1

Gene Ontology
Bibliography

Related articles in PubMed

In vitro and in vivo studies of oxidative stress responses against acrylamide toxicity in zebrafish.
Komoike Y, Matsuoka M
Journal of hazardous materials365430-439(2019 Mar)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Huang HT, Kathrein KL, Barton A, Gitlin Z, Huang YH, Ward TP, Hofmann O, Dibiase A, Song A, Tyekucheva S, Hide W, Zhou Y, Zon LI
Nature cell biology15(12)1516-25(2013 Dec)

A high level of liver-specific expression of oncogenic Kras(V12) drives robust liver tumorigenesis in transgenic zebrafish.
Nguyen AT, Emelyanov A, Koh CH, Spitsbergen JM, Lam SH, Mathavan S, Parinov S, Gong Z
Disease models & mechanisms4(6)801-13(2011 Nov)

Reactive oxygen species generation and expression of DNA repair-related genes after copper exposure in zebrafish (Danio rerio) ZFL cells.
Sandrini JZ, Bianchini A, Trindade GS, Nery LE, Marins LF
Aquatic toxicology (Amsterdam, Netherlands)95(4)285-91(2009 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following nbn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nbn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa38437	NM_001014819.1 Latest version!	Danio rerio nibrin (nbn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nbn ( NM_001014819.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001014819.1 Danio rerio nibrin (nbn), mRNA.

Price & Availability↑

CloneID	ODa38437
Clone ID Related Accession (Same CDS sequence)	NM_001014819.1
Accession Version	NM_001014819.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577203200000
Organism	Danio rerio(zebrafish)
Product	nibrin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY858826.1. ##Evidence-Data-START## Transcript exon combination :: AY858826.1, BC162723.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTGGAAAC TGCAGCCAAC CGAATCTGGA GGGGAAAGTG TCATTCTGCT GGCTGGACAG 
GAGTATGTGG TTGGCCGTAA GAATTGTGAG ATCCTGCTGA CCAATGATCA GTCCATCAGC 
CGTGTTCATG CTGTCCTCAC TGTCACTGAG CAGGCAGTCA CTCTGAAGGA CAGCTCTAAA 
TATGGAACTT TTGTCAATGG TGAGAAGCTT GAGTCTGGCT CCACAAAGAC CTTGCAGACA 
GGATACAAAA TCACATTTGG AGTCTTCCAG AGCAAATTCA GTCTGGAGAA AGAGTGTATT 
GTGGTTTGCT CCTCGTGTGT GGATAATGAA GGGAAAGTCA CCCTGTCTCA AGATATTCGC 
TCTGTTGGAG GACGACTTGT CAGTTCCTGG ACCTCTGACT GCACACATCT AGTCATGCCC 
ACTGTAAAAG TCACCATAAA GACGATCTGT GCGTTGCTAT GCTGTCGACC CATAGTAAAA 
CCTGCGTTCT TCAGTGCGTT TAGCAAAGCT GTGCAGCAGA AATTGCCCCT GCCTAAAGCT 
GAGAGATTTA GGCCTCAAAT TGATGAGCCC AGCCTGGCTA GAGATGAAGT GGATCTGGGT 
GCACGACCTG AGAGAAAGAG CCTCTTCAAG GGGAAAACAT TCCTGTTTTT GAGCTCTAAA 
CAGATGAAGC GTTTGAGTGT GGCAGTGAGT TGTGGTGGTG GTGTGAGTCA GCTGCTGGAC 
GAGGGCGCTC TGCCTGTCTC ACTCCTGGAG TCCAGCAGCA CATGTGTGCT CGACATGATC 
TCAGGAAACT CACAGCCTGT GATTTCTCCA GCCTCCAAGA AGTGGTTGGA TTCTGTGGGC 
CAAATTCTGC ACAGAAAAGG TTTGCGCTTT ATCACTGAAT CTGAGGTTGG ATTGGCTGCC 
ATTCATGTCA GTAATCAGAC ATACTGCAAT CCCTGCTCTT CTTTGCAATC AGAATCAGTG 
AAAACGAATC CAGTCTTTGC CTCAGCAACA CTTTCTCAGA GCACGGCTGT GGATGAGACT 
GCGCTCGCGG CTCCTTCCCA GAATATCACA GCCTATGTCG TCAACACTGA AATCTCACAG 
GACCAAAGCA GGATGGTTAC AAGTGGGATC AGTGCAGTTG GAGAGACCCC AGAGAAAACC 
AATCCCACAC AGAAAGCCTC AACAACCAAT AAACCCTTAT CCCTCGGTCA GGAACCTTCC 
TCTACCCGCA TCGTCCAAGA AACAGTGATG TCCTCTGAGA GCTTCAGTGT GGTTGAATCT 
GAACAGAAGA TGAAGAAAGG GTCTGTTGTC TCAGCCAGGG GAAGAGTTGA AGGTCCTGTA 
AAACAGAAAG CGCCTTCCAG TGGCAACACC ACATTAAAAC ATTCTCCCCA GAAACAGACA 
GCCCTCACCA GCTTCTTCCA GCCTTCCAGC AAGAAAAGGC CGCGTGAAAG CAGTGCCAGT 
TCTGTCCAGC CAGAACCCAA ATTCTTCAAG AAAGACATCA AAGATAATGA AGATGACATC 
CAGCAAAGCT CTTCTGTCAA TAGATCTCAC AAAACCTCAT CAGAGGAGAC CAGTCTAGGT 
CAGGCTTGTG GAACTGGTCA AAACTCTAGC AGCAAGAAAA GAAAGGAACC TGAGCAGGAC 
ACCCTATTAG GCGCAGAAGA ACCAACAGCC GCTGATGATT TAGAAATGTC ACTTGAAGAG 
CTTGAATTCT TAATGTCCGA CGAGATGGAT GAGCCTCCGC AGACTGCAGC TAATAAAAAA 
CAACGTCTTG AGTCAGGACT GACTAGCAAA ATCAATAGCG AACAATTATC GAATCAGCAA 
GAGGTCACTG AGTCTAAAGG CAGGAAAGGG GAGAAGAATC AGCAAAGCAG TTCCAGCAAT 
ATTCAGAGCA TGCAGTTGGA CAGAGCAGGA CCAGCTGTAA CAAATCAGGA CACACAAACA 
CAGTCAAAGA GGTCACCTCC AGATCTGGAA GCACATAGTT CTGCAAACAA GGGGCCAACA 
AAAAACAAAA CACCCGAGCT TGAAGAGGTC AAAAAGGAGG AAGTGTCCTT TGTTGTGAAT 
TCAAGGCCCC AAAATGGCAT TTCCCAGACC TCTGAGGCTG TTCTCAAACA GGAAATGCGG 
GCCTCAACCT CCAATTCCGG ACCTAAGAAT GACCCCGATC TGCCCAGGAA GCTACTGCAG 
GTTCAGTTTA TGTCTCTAAC AGTGAACAAC TCTTCCAGAT CAAGACCAGG CCCATTACAG 
ACACACAATC CAAATGACAA GAACGTCAAA AGATTTCGCA AGAAAAATGT CCCTGGATTT 
GATGGGCTGC CTAAGATAAT TGGAGGCTCG GATCTGGTGG CTCATAATCG CAGTAAGCAC 
TCTGAACTAG AGGAATGGCT GAGACAGGCA GCAGAGGAGG AGAAGCTGAA CGAACGAGAG 
GAGTATTTGG GAGACGATTT GTTCAGGTAC AACCCTAGGC CTGCAAAGAA ACGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014819.1
CDS	1..2457
Translation	MWKLQPTESGGESVILLAGQEYVVGRKNCEILLTNDQSISRVHAVLTVTEQAVTLKDSSKYGTFVNGEKLESGSTKTLQTGYKITFGVFQSKFSLEKECIVVCSSCVDNEGKVTLSQDIRSVGGRLVSSWTSDCTHLVMPTVKVTIKTICALLCCRPIVKPAFFSAFSKAVQQKLPLPKAERFRPQIDEPSLARDEVDLGARPERKSLFKGKTFLFLSSKQMKRLSVAVSCGGGVSQLLDEGALPVSLLESSSTCVLDMISGNSQPVISPASKKWLDSVGQILHRKGLRFITESEVGLAAIHVSNQTYCNPCSSLQSESVKTNPVFASATLSQSTAVDETALAAPSQNITAYVVNTEISQDQSRMVTSGISAVGETPEKTNPTQKASTTNKPLSLGQEPSSTRIVQETVMSSESFSVVESEQKMKKGSVVSARGRVEGPVKQKAPSSGNTTLKHSPQKQTALTSFFQPSSKKRPRESSASSVQPEPKFFKKDIKDNEDDIQQSSSVNRSHKTSSEETSLGQACGTGQNSSSKKRKEPEQDTLLGAEEPTAADDLEMSLEELEFLMSDEMDEPPQTAANKKQRLESGLTSKINSEQLSNQQEVTESKGRKGEKNQQSSSSNIQSMQLDRAGPAVTNQDTQTQSKRSPPDLEAHSSANKGPTKNKTPELEEVKKEEVSFVVNSRPQNGISQTSEAVLKQEMRASTSNSGPKNDPDLPRKLLQVQFMSLTVNNSSRSRPGPLQTHNPNDKNVKRFRKKNVPGFDGLPKIIGGSDLVAHNRSKHSELEEWLRQAAEEEKLNEREEYLGDDLFRYNPRPAKKR

Target ORF information:

RefSeq Version	NM_001014819.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nibrin (nbn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014819.1

ORF Insert Sequence:

ATGTGGAAAC TGCAGCCAAC CGAATCTGGA GGGGAAAGTG TCATTCTGCT GGCTGGACAG 
GAGTATGTGG TTGGCCGTAA GAATTGTGAG ATCCTGCTGA CCAATGATCA GTCCATCAGC 
CGTGTTCATG CTGTCCTCAC TGTCACTGAG CAGGCAGTCA CTCTGAAGGA CAGCTCTAAA 
TATGGAACTT TTGTCAATGG TGAGAAGCTT GAGTCTGGCT CCACAAAGAC CTTGCAGACA 
GGATACAAAA TCACATTTGG AGTCTTCCAG AGCAAATTCA GTCTGGAGAA AGAGTGTATT 
GTGGTTTGCT CCTCGTGTGT GGATAATGAA GGGAAAGTCA CCCTGTCTCA AGATATTCGC 
TCTGTTGGAG GACGACTTGT CAGTTCCTGG ACCTCTGACT GCACACATCT AGTCATGCCC 
ACTGTAAAAG TCACCATAAA GACGATCTGT GCGTTGCTAT GCTGTCGACC CATAGTAAAA 
CCTGCGTTCT TCAGTGCGTT TAGCAAAGCT GTGCAGCAGA AATTGCCCCT GCCTAAAGCT 
GAGAGATTTA GGCCTCAAAT TGATGAGCCC AGCCTGGCTA GAGATGAAGT GGATCTGGGT 
GCACGACCTG AGAGAAAGAG CCTCTTCAAG GGGAAAACAT TCCTGTTTTT GAGCTCTAAA 
CAGATGAAGC GTTTGAGTGT GGCAGTGAGT TGTGGTGGTG GTGTGAGTCA GCTGCTGGAC 
GAGGGCGCTC TGCCTGTCTC ACTCCTGGAG TCCAGCAGCA CATGTGTGCT CGACATGATC 
TCAGGAAACT CACAGCCTGT GATTTCTCCA GCCTCCAAGA AGTGGTTGGA TTCTGTGGGC 
CAAATTCTGC ACAGAAAAGG TTTGCGCTTT ATCACTGAAT CTGAGGTTGG ATTGGCTGCC 
ATTCATGTCA GTAATCAGAC ATACTGCAAT CCCTGCTCTT CTTTGCAATC AGAATCAGTG 
AAAACGAATC CAGTCTTTGC CTCAGCAACA CTTTCTCAGA GCACGGCTGT GGATGAGACT 
GCGCTCGCGG CTCCTTCCCA GAATATCACA GCCTATGTCG TCAACACTGA AATCTCACAG 
GACCAAAGCA GGATGGTTAC AAGTGGGATC AGTGCAGTTG GAGAGACCCC AGAGAAAACC 
AATCCCACAC AGAAAGCCTC AACAACCAAT AAACCCTTAT CCCTCGGTCA GGAACCTTCC 
TCTACCCGCA TCGTCCAAGA AACAGTGATG TCCTCTGAGA GCTTCAGTGT GGTTGAATCT 
GAACAGAAGA TGAAGAAAGG GTCTGTTGTC TCAGCCAGGG GAAGAGTTGA AGGTCCTGTA 
AAACAGAAAG CGCCTTCCAG TGGCAACACC ACATTAAAAC ATTCTCCCCA GAAACAGACA 
GCCCTCACCA GCTTCTTCCA GCCTTCCAGC AAGAAAAGGC CGCGTGAAAG CAGTGCCAGT 
TCTGTCCAGC CAGAACCCAA ATTCTTCAAG AAAGACATCA AAGATAATGA AGATGACATC 
CAGCAAAGCT CTTCTGTCAA TAGATCTCAC AAAACCTCAT CAGAGGAGAC CAGTCTAGGT 
CAGGCTTGTG GAACTGGTCA AAACTCTAGC AGCAAGAAAA GAAAGGAACC TGAGCAGGAC 
ACCCTATTAG GCGCAGAAGA ACCAACAGCC GCTGATGATT TAGAAATGTC ACTTGAAGAG 
CTTGAATTCT TAATGTCCGA CGAGATGGAT GAGCCTCCGC AGACTGCAGC TAATAAAAAA 
CAACGTCTTG AGTCAGGACT GACTAGCAAA ATCAATAGCG AACAATTATC GAATCAGCAA 
GAGGTCACTG AGTCTAAAGG CAGGAAAGGG GAGAAGAATC AGCAAAGCAG TTCCAGCAAT 
ATTCAGAGCA TGCAGTTGGA CAGAGCAGGA CCAGCTGTAA CAAATCAGGA CACACAAACA 
CAGTCAAAGA GGTCACCTCC AGATCTGGAA GCACATAGTT CTGCAAACAA GGGGCCAACA 
AAAAACAAAA CACCCGAGCT TGAAGAGGTC AAAAAGGAGG AAGTGTCCTT TGTTGTGAAT 
TCAAGGCCCC AAAATGGCAT TTCCCAGACC TCTGAGGCTG TTCTCAAACA GGAAATGCGG 
GCCTCAACCT CCAATTCCGG ACCTAAGAAT GACCCCGATC TGCCCAGGAA GCTACTGCAG 
GTTCAGTTTA TGTCTCTAAC AGTGAACAAC TCTTCCAGAT CAAGACCAGG CCCATTACAG 
ACACACAATC CAAATGACAA GAACGTCAAA AGATTTCGCA AGAAAAATGT CCCTGGATTT 
GATGGGCTGC CTAAGATAAT TGGAGGCTCG GATCTGGTGG CTCATAATCG CAGTAAGCAC 
TCTGAACTAG AGGAATGGCT GAGACAGGCA GCAGAGGAGG AGAAGCTGAA CGAACGAGAG 
GAGTATTTGG GAGACGATTT GTTCAGGTAC AACCCTAGGC CTGCAAAGAA ACGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

In vitro and in vivo studies of oxidative stress responses against acrylamide toxicity in zebrafish.
Journal of hazardous materials365430-439(2019 Mar)
Komoike Y,Matsuoka M

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Nature cell biology15(12)1516-25(2013 Dec)
Huang HT,Kathrein KL,Barton A,Gitlin Z,Huang YH,Ward TP,Hofmann O,Dibiase A,Song A,Tyekucheva S,Hide W,Zhou Y,Zon LI

A high level of liver-specific expression of oncogenic Kras(V12) drives robust liver tumorigenesis in transgenic zebrafish.
Disease models & mechanisms4(6)801-13(2011 Nov)
Nguyen AT,Emelyanov A,Koh CH,Spitsbergen JM,Lam SH,Mathavan S,Parinov S,Gong Z

Reactive oxygen species generation and expression of DNA repair-related genes after copper exposure in zebrafish (Danio rerio) ZFL cells.
Aquatic toxicology (Amsterdam, Netherlands)95(4)285-91(2009 Dec)
Sandrini JZ,Bianchini A,Trindade GS,Nery LE,Marins LF

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nbn cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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