MAPK7 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAPK7
Entrez Gene ID	541323
Full Name	mitogen-activated protein kinase 7
Synonyms	bmk1,erk5,wu:fb73b02,zgc:113111
General protein information	Preferred Names mitogen-activated protein kinase 7 Names mitogen-activated protein kinase 7 extracellular signal-regulated kinase 5 fb73b02
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005156002.4	XP_005156059.1	mitogen-activated protein kinase 7 isoform X1
NM_001013451.3	NP_001013469.2	mitogen-activated protein kinase 7
XM_005156002.3	XP_005156059.1	mitogen-activated protein kinase 7 isoform X1

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	MAPK7	XP_001099826.1
Canis lupus familiaris (dog)	MAPK7	XP_546651.3
Arabidopsis thaliana (thale cress)	MPK11	NP_001117210.1
Rattus norvegicus (Norway rat)	LOC100912585	XP_006246619.1
Kluyveromyces lactis	KLLA0B11902g	XP_452059.1
Oryza sativa (rice)	Os10g0533600	NP_001065156.1
Schizosaccharomyces pombe (fission yeast)	pmk1	NP_595289.1
Pan troglodytes (chimpanzee)	MAPK7	XP_511332.3
Mus musculus (house mouse)	Mapk7	NP_035971.1
Saccharomyces cerevisiae (baker's yeast)	SLT2	NP_011895.1
Arabidopsis thaliana (thale cress)	MPK4	NP_192046.1
Rattus norvegicus (Norway rat)	Mapk7	NP_001178476.1
Danio rerio (zebrafish)	mapk7	NP_001013469.2
Xenopus tropicalis (tropical clawed frog)	mapk7	NP_001072332.1
Eremothecium gossypii	AGOS_AER232C	NP_985089.2
Magnaporthe oryzae (rice blast fungus)	MGG_04943	XP_003712437.1
Homo sapiens (human)	MAPK7	NP_002740.2
Bos taurus (cattle)	MAPK7	NP_001092550.1
Neurospora crassa	NCU11376	XP_958040.1

Gene Ontology
Bibliography

Related articles in PubMed

The Progress of CRISPR/Cas9-Mediated Gene Editing in Generating Mouse/Zebrafish Models of Human Skeletal Diseases.
Wu N, Liu B, Du H, Zhao S, Li Y, Cheng X, Wang S, Lin J, Zhou J, , Qiu G, Wu Z, Zhang J
Computational and structural biotechnology journal17954-962(2019)

The discrepancy function of NLRC5 isoforms in antiviral and antibacterial immune responses.
Cao L, Wu XM, Hu YW, Xue NN, Nie P, Chang MX
Developmental and comparative immunology84153-163(2018 07)

Mutant MAPK7-Induced Idiopathic Scoliosis is Linked to Impaired Osteogenesis.
Zhou T, Chen C, Xu C, Zhou H, Gao B, Su D, Liao Z, Li Y, Yang S, Su P
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology48(3)880-890(2018)

Blood Flow Regulates Zebrafish Caudal Vein Plexus Angiogenesis by ERK5-klf2a-nos2b Signaling.
Xie X, Zhou T, Wang Y, Chen H, Lei D, Huang L, Wang Y, Jin X, Sun T, Tan J, Yin T, Huang J, Gregersen H, Wang G
Current molecular medicine18(1)3-14(2018)

Rare coding variants in MAPK7 predispose to adolescent idiopathic scoliosis.
Gao W, Chen C, Zhou T, Yang S, Gao B, Zhou H, Lian C, Wu Z, Qiu X, Yang X, Alattar E, Liu W, Su D, Sun S, Chen Y, Cheung KMC, Song Y, Luk KKD, Chan D, Sham PC, Xing C, Khor CC, Liu G, Yang J, Deng Y, Hao D, Huang D, Li QZ, Xu C, Su P
Human mutation38(11)1500-1510(2017 11)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MAPK7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAPK7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa17535	NM_001013451.3 Latest version!	Danio rerio mitogen-activated protein kinase 7 (mapk7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa17534	XM_005156002.4 Latest version!	Danio rerio mitogen-activated protein kinase 7 (mapk7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
ODa17534	XM_005156002.3	Danio rerio mitogen-activated protein kinase 7 (mapk7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAPK7 ( NM_001013451.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001013469.2 Danio rerio mitogen-activated protein kinase 7 (mapk7), mRNA.

Price & Availability↑

CloneID	ODa17535
Clone ID Related Accession (Same CDS sequence)	NM_001013451.3
Accession Version	NM_001013451.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576252800000
Organism	Danio rerio(zebrafish)
Product	mitogen-activated protein kinase 7
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DQ360072.1 and AL954132.14. On May 26, 2016 this sequence version replaced NM_001013451.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: DQ360072.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCATCCA ATAAGATGGA TGAGACAAAT AACAGTCACA CAGCAGGAGA AGCCTCAGAA 
GGCGACAGCA GGAGAGAGAG CAACATTGAA AAGGTCCAGC AAAGAGGCGT TACAGAAGGA 
CCAGCTCAAC CCAGCAATGA TACGTCCACA GTTACTGATG ACACTACTGT CGCAGACAAG 
AACTTGGCCC TGCTCAAAGC CCACTCCCTG GATGTGAAGT TTGAAGTCGG GGATGAATAT 
GACATTATTG AGACCATTGG TACAGGTGCA TATGGTGTGG TCTCATCAGC ACGAAGACGG 
GATAATGGCC AGCAGGTGGC AATAAAGAAG ATCCCCAATG CCTTTGAGGT TGTGACGAAT 
GCCAAACGCA CGCTGCGAGA GCTCAAAATA CTGAAGCACT TCAAACACGA CAACATCATC 
GCCATTAAAG ATATTCTCCA GCCCGTGGTC CCTCATTCAG CGTTCAAATC AGTGTACGTG 
GTTCTTGACC TCATGGAGAG CGACCTACAT CAGATCATTC ACTCGCGGCA GCCTTTGACT 
CCAGAACACA CACGCTACTT TCTATATCAG CTGTTAAGAG GCTTGAAATA CATTCATTCA 
GCCAACGTCA TTCACCGCGA CCTCAAGCCA TCCAACCTGC TGGTCAATGA AAACTGCGAG 
CTAAAGATCG GGGACTTCGG AATGGCACGG GGATTAAGTG CGGTCTACTC TGAAGAGTCG 
CGGTCCTTTA TGACCGAGTA CGTGGCCACC CGCTGGTATC GTGCTCCTGA GCTCATGCTG 
TCGCTCCACC ACTACAGCCT GGCCATCGAC CTCTGGTCCG TAGGCTGCAT CTTCGGTGAG 
ATGCTCGGTA GGAGGCAGAT GTTTCCGGGG AAAAACTACG TGCATCAGTT GCAGCTGATT 
CTTTCAGTTT TAGGGACGCC ACCCGAAAGC ATTGTTGGCT CAATTGGGTC AGATAGGGTG 
CGCTCTTATG TGCGGAGCCT TCCATCCAAA GCACCAGAAC CGCTTGCAGC CCTGTATCCA 
CAAGCTGAGC CCAGCGCCTT GAACTTGTTG GCTGCAATGC TCCGCTTTGA CCCTCGTGAA 
AGAATCAGTG CATGTCAAGC ACTGGAACAC CCGTATCTCT CCAAGTACCA CGACCCAGAT 
GACGAACCGG TGTGCGTGCC TGCTTTCAAT TTTGAGTTCG ACCGGCAGCC CATGGGCAAA 
GAGCAAATCA AAGAAGCGAT ACTAACAGAG ATCCAGGACT TTCATAAGAA AAAGCAGGGC 
GTGAGGAAGA AGATCCAGTT TAAGCCGCTC CATTCTTCTG GATCGGCCTT GTCGGTAAGC 
TGTCAGGATG TGGACATGCC CAGTGCGAAC TCTGACGGCC AGCCGGAAAC CATTGATCTG 
ACCACTCCCA CAGACAAGGA CACGTCTTTT ACTGAGCCTG CGATAGACTG CAGGAAGGAT 
AAGAGTGTGT TATCCAATTA CACTGCTCCA TCTCGATCCG TCTCTCAGAC TCCACCCACA 
AGTCTTCCAC AGTCAGTCCC TTCACTCTCT CTCTCACAGT CCCAGGTGCA GTCGCTCTCT 
CAAAATCTCT CTCGTTCGCT TGGAAAAGGA GGAAAACCGA CTGCTGCGGA AGGTACGAAA 
AAAGAGGGAG CAATATCTGA AGACACAAAA GCAGCTCTCA AAGCTGCCTT GCAGAAATCT 
GCGCTAAGAC AGAAGGCACG AGATGAAGGC ACTGCAGCTC TTGGTATAGA TTTAGTCAGA 
GGAGCTTCTG TTGGTTTATC CTCATCCATA TCCTCCTCCA ACTCCTGTCC AGAGCAGAGG 
AGGCCCATCA CGGCCCAGGA GAGACAACGG GAGAGGGAAG AGAAGCGGAA GAAAAGGCAG 
GAGCGGGCCA TGGAGCGAAA GAGGAAGCTA AAAGAAAAGG AAAAAAAGGA AGGGAAACAT 
GGAGAATCGC TGGGTGGCGT TTTACTCAGC GATAATGACA AGATGCTGCT GCAGCGCTGG 
GTACGAATGA TGGACAAACC AGCAGACAAA TCACAAGGCA TAGAAACCAG TAATGCCAAA 
ACCAGCGACA GTAATATGGC CCAAATACAG CCAGCAACAG GAAACAACAT TATAAAAACA 
AACTCCAATG GGGGGGAATC AGTGCTGCCA AAACAGCCCA ACAAAGCACA GGTTTTCAAA 
CCGCCACAGT TGGTGTCTCA GATGGGACAG CAGCCTCCAG GTGCTGCAGT TCAACCCTGC 
CTGAGTCTCA CTGACACGGG ACAGTCTTCG AACAGCATCC CTGATATCCA CACCGTCACT 
CAGCAGCTCT CCAAATCACA GGTTGAAGAC ATTTTGCCGC CAGTTTTCTC AGTGACTCCT 
AAAGGCAGTG GAGCCGGTTA TGGTGTGGGC TTTGACCTCG ATGACCTCCT CACTCAGTCT 
CTAACTGAAC TCCAACATTC TGACCTCAGG GAAAGTCACA ATGACCTAGC CCCTCTGTCT 
GCATCCCTGC TTTCTGACTG GCTGGAGGTG CATCGCATGA CCCCTGCGGA CATGGAGTCA 
CTTCAGCAGG AGCTTCAACT GGGATCTCCC ATGATCCTCT CAGACAACTC TACGCTTCCT 
GATACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001013469.2
CDS	1..2589
Translation	MASNKMDETNNSHTAGEASEGDSRRESNIEKVQQRGVTEGPAQPSNDTSTVTDDTTVADKNLALLKAHSLDVKFEVGDEYDIIETIGTGAYGVVSSARRRDNGQQVAIKKIPNAFEVVTNAKRTLRELKILKHFKHDNIIAIKDILQPVVPHSAFKSVYVVLDLMESDLHQIIHSRQPLTPEHTRYFLYQLLRGLKYIHSANVIHRDLKPSNLLVNENCELKIGDFGMARGLSAVYSEESRSFMTEYVATRWYRAPELMLSLHHYSLAIDLWSVGCIFGEMLGRRQMFPGKNYVHQLQLILSVLGTPPESIVGSIGSDRVRSYVRSLPSKAPEPLAALYPQAEPSALNLLAAMLRFDPRERISACQALEHPYLSKYHDPDDEPVCVPAFNFEFDRQPMGKEQIKEAILTEIQDFHKKKQGVRKKIQFKPLHSSGSALSVSCQDVDMPSANSDGQPETIDLTTPTDKDTSFTEPAIDCRKDKSVLSNYTAPSRSVSQTPPTSLPQSVPSLSLSQSQVQSLSQNLSRSLGKGGKPTAAEGTKKEGAISEDTKAALKAALQKSALRQKARDEGTAALGIDLVRGASVGLSSSISSSNSCPEQRRPITAQERQREREEKRKKRQERAMERKRKLKEKEKKEGKHGESLGGVLLSDNDKMLLQRWVRMMDKPADKSQGIETSNAKTSDSNMAQIQPATGNNIIKTNSNGGESVLPKQPNKAQVFKPPQLVSQMGQQPPGAAVQPCLSLTDTGQSSNSIPDIHTVTQQLSKSQVEDILPPVFSVTPKGSGAGYGVGFDLDDLLTQSLTELQHSDLRESHNDLAPLSASLLSDWLEVHRMTPADMESLQQELQLGSPMILSDNSTLPDT

Target ORF information:

RefSeq Version	NM_001013451.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mitogen-activated protein kinase 7 (mapk7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001013451.3

ORF Insert Sequence:

ATGGCATCCA ATAAGATGGA TGAGACAAAT AACAGTCACA CAGCAGGAGA AGCCTCAGAA 
GGCGACAGCA GGAGAGAGAG CAACATTGAA AAGGTCCAGC AAAGAGGCGT TACAGAAGGA 
CCAGCTCAAC CCAGCAATGA TACGTCCACA GTTACTGATG ACACTACTGT CGCAGACAAG 
AACTTGGCCC TGCTCAAAGC CCACTCCCTG GATGTGAAGT TTGAAGTCGG GGATGAATAT 
GACATTATTG AGACCATTGG TACAGGTGCA TATGGTGTGG TCTCATCAGC ACGAAGACGG 
GATAATGGCC AGCAGGTGGC AATAAAGAAG ATCCCCAATG CCTTTGAGGT TGTGACGAAT 
GCCAAACGCA CGCTGCGAGA GCTCAAAATA CTGAAGCACT TCAAACACGA CAACATCATC 
GCCATTAAAG ATATTCTCCA GCCCGTGGTC CCTCATTCAG CGTTCAAATC AGTGTACGTG 
GTTCTTGACC TCATGGAGAG CGACCTACAT CAGATCATTC ACTCGCGGCA GCCTTTGACT 
CCAGAACACA CACGCTACTT TCTATATCAG CTGTTAAGAG GCTTGAAATA CATTCATTCA 
GCCAACGTCA TTCACCGCGA CCTCAAGCCA TCCAACCTGC TGGTCAATGA AAACTGCGAG 
CTAAAGATCG GGGACTTCGG AATGGCACGG GGATTAAGTG CGGTCTACTC TGAAGAGTCG 
CGGTCCTTTA TGACCGAGTA CGTGGCCACC CGCTGGTATC GTGCTCCTGA GCTCATGCTG 
TCGCTCCACC ACTACAGCCT GGCCATCGAC CTCTGGTCCG TAGGCTGCAT CTTCGGTGAG 
ATGCTCGGTA GGAGGCAGAT GTTTCCGGGG AAAAACTACG TGCATCAGTT GCAGCTGATT 
CTTTCAGTTT TAGGGACGCC ACCCGAAAGC ATTGTTGGCT CAATTGGGTC AGATAGGGTG 
CGCTCTTATG TGCGGAGCCT TCCATCCAAA GCACCAGAAC CGCTTGCAGC CCTGTATCCA 
CAAGCTGAGC CCAGCGCCTT GAACTTGTTG GCTGCAATGC TCCGCTTTGA CCCTCGTGAA 
AGAATCAGTG CATGTCAAGC ACTGGAACAC CCGTATCTCT CCAAGTACCA CGACCCAGAT 
GACGAACCGG TGTGCGTGCC TGCTTTCAAT TTTGAGTTCG ACCGGCAGCC CATGGGCAAA 
GAGCAAATCA AAGAAGCGAT ACTAACAGAG ATCCAGGACT TTCATAAGAA AAAGCAGGGC 
GTGAGGAAGA AGATCCAGTT TAAGCCGCTC CATTCTTCTG GATCGGCCTT GTCGGTAAGC 
TGTCAGGATG TGGACATGCC CAGTGCGAAC TCTGACGGCC AGCCGGAAAC CATTGATCTG 
ACCACTCCCA CAGACAAGGA CACGTCTTTT ACTGAGCCTG CGATAGACTG CAGGAAGGAT 
AAGAGTGTGT TATCCAATTA CACTGCTCCA TCTCGATCCG TCTCTCAGAC TCCACCCACA 
AGTCTTCCAC AGTCAGTCCC TTCACTCTCT CTCTCACAGT CCCAGGTGCA GTCGCTCTCT 
CAAAATCTCT CTCGTTCGCT TGGAAAAGGA GGAAAACCGA CTGCTGCGGA AGGTACGAAA 
AAAGAGGGAG CAATATCTGA AGACACAAAA GCAGCTCTCA AAGCTGCCTT GCAGAAATCT 
GCGCTAAGAC AGAAGGCACG AGATGAAGGC ACTGCAGCTC TTGGTATAGA TTTAGTCAGA 
GGAGCTTCTG TTGGTTTATC CTCATCCATA TCCTCCTCCA ACTCCTGTCC AGAGCAGAGG 
AGGCCCATCA CGGCCCAGGA GAGACAACGG GAGAGGGAAG AGAAGCGGAA GAAAAGGCAG 
GAGCGGGCCA TGGAGCGAAA GAGGAAGCTA AAAGAAAAGG AAAAAAAGGA AGGGAAACAT 
GGAGAATCGC TGGGTGGCGT TTTACTCAGC GATAATGACA AGATGCTGCT GCAGCGCTGG 
GTACGAATGA TGGACAAACC AGCAGACAAA TCACAAGGCA TAGAAACCAG TAATGCCAAA 
ACCAGCGACA GTAATATGGC CCAAATACAG CCAGCAACAG GAAACAACAT TATAAAAACA 
AACTCCAATG GGGGGGAATC AGTGCTGCCA AAACAGCCCA ACAAAGCACA GGTTTTCAAA 
CCGCCACAGT TGGTGTCTCA GATGGGACAG CAGCCTCCAG GTGCTGCAGT TCAACCCTGC 
CTGAGTCTCA CTGACACGGG ACAGTCTTCG AACAGCATCC CTGATATCCA CACCGTCACT 
CAGCAGCTCT CCAAATCACA GGTTGAAGAC ATTTTGCCGC CAGTTTTCTC AGTGACTCCT 
AAAGGCAGTG GAGCCGGTTA TGGTGTGGGC TTTGACCTCG ATGACCTCCT CACTCAGTCT 
CTAACTGAAC TCCAACATTC TGACCTCAGG GAAAGTCACA ATGACCTAGC CCCTCTGTCT 
GCATCCCTGC TTTCTGACTG GCTGGAGGTG CATCGCATGA CCCCTGCGGA CATGGAGTCA 
CTTCAGCAGG AGCTTCAACT GGGATCTCCC ATGATCCTCT CAGACAACTC TACGCTTCCT 
GATACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAPK7 ( XM_005156002.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005156059.1 Danio rerio mitogen-activated protein kinase 7 (mapk7), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa17534
Clone ID Related Accession (Same CDS sequence)	XM_005156002.4 , XM_005156002.3
Accession Version	XM_005156002.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	mitogen-activated protein kinase 7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005156002.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCCA ATAAGATGGA TGAGACAAAT AACAGTCACA CAGCAGGAGA AGCCTCAGAA 
GGCGACAGCA GGAGAGAGAG CAACATTGAA AAGGTCCAGC AAAGAGGCGT TACAGAAGGA 
CCAGCTCAAC CCAGCAATGA TACGTCCACA GTTACTGATG ACACTACTGT CGCAGACAAG 
AACTTGGCCC TGCTCAAAGC CCACTCCCTG GATGTGAAGT TTGAAGTCGG GGATGAATAT 
GACATTATTG AGACCATTGG TACAGGTGCA TATGGTGTGG TCTCATCAGC ACGAAGACGG 
GATAATGGCC AGCAGGTGGC AATAAAGAAG ATCCCCAATG CCTTTGAGGT TGTGACGAAT 
GCCAAACGCA CGCTGCGAGA GCTCAAAATA CTGAAGCACT TCAAACACGA CAACATCATC 
GCCATTAAAG ATATTCTCCA GCCCGTGGTC CCTCATTCAG CGTTCAAATC AGTGTACGTG 
GTTCTTGACC TCATGGAGAG CGACCTACAT CAGATCATTC ACTCGCGGCA GCCTTTGACT 
CCAGAACACA CACGCTACTT TCTATATCAG CTGTTAAGAG GCTTGAAATA CATTCATTCA 
GCCAACGTCA TTCACCGCGA CCTCAAGCCA TCCAACCTGC TGGTCAATGA AAACTGCGAG 
CTAAAGATCG GGGACTTCGG AATGGCACGG GGATTAAGTG CGGTCTACTC TGAAGAGTCG 
CGGTCCTTTA TGACCGAGTA CGTGGCCACC CGCTGGTATC GTGCTCCTGA GCTCATGCTG 
TCGCTCCACC ACTACAGCCT GGCCATCGAC CTCTGGTCCG TAGGCTGCAT CTTCGGTGAG 
ATGCTCGGTA GGAGGCAGAT GTTTCCGGGG AAAAACTACG TGCATCAGTT GCAGCTGATT 
CTTTCAGTTT TAGGGACGCC ACCCGAAAGC ATTGTTGGCT CAATTGGGTC AGATAGGGTG 
CGCTCTTATG TGCGGAGCCT TCCATCCAAA GCACCAGAAC CGCTTGCAGC CCTGTATCCA 
CAAGCTGAGC CCAGCGCCTT GAACTTGTTG GCTGCAATGC TCCGCTTTGA CCCTCGTGAA 
AGAATCAGTG CATGTCAAGC ACTGGAACAC CCGTATCTCT CCAAGTACCA CGACCCAGAT 
GACGAACCGG TGTGCGTGCC TGCTTTCAAT TTTGAGTTCG ACCGGCAGCC CATGGGCAAA 
GAGCAAATCA AAGAAGCGAT ACTAACAGAG ATCCAGGACT TTCATAAGAA AAAGCAGGGC 
GTGAGGAAGA AGATCCAGTT TAAGCCGCTC CATTCTTCTG GATCGGCCTT GGTCGGTGTT 
TCTAGTAACA GCCATTGCAC AGCGCAACAG TCTCAACACC TGAAGGAGAT GTCACAAACT 
CACCAGCAGC ACAGTCTCAA CTTCAAACCA GCAGACACTA CTCAAATGAA TGTAAACTCG 
TTTCAAACTC CACATCTTAT TGGTCAAATT TTGCCACATT TGGAACAGTC GGTAAGCTGT 
CAGGATGTGG ACATGCCCAG TGCGAACTCT GACGGCCAGC CGGAAACCAT TGATCTGACC 
ACTCCCACAG ACAAGGACAC GTCTTTTACT GAGCCTGCGA TAGACTGCAG GAAGGATAAG 
AGTGTGTTAT CCAATTACAC TGCTCCATCT CGATCCGTCT CTCAGACTCC ACCCACAAGT 
CTTCCACAGT CAGTCCCTTC ACTCTCTCTC TCACAGTCCC AGGTGCAGTC GCTCTCTCAA 
AATCTCTCTC GTTCGCTTGG AAAAGGAGGA AAACCGACTG CTGCGGAAGG TACGAAAAAA 
GAGGGAGCAA TATCTGAAGA CACAAAAGCA GCTCTCAAAG CTGCCTTGCA GAAATCTGCG 
CTAAGACAGA AGGCACGAGA TGAAGGCACT GCAGCTCTTG GTATAGATTT AGTCAGAGGA 
GCTTCTGTTG GTTTATCCTC ATCCATATCC TCCTCCAACT CCTGTCCAGA GCAGAGGAGG 
CCCATCACGG CCCAGGAGAG ACAACGGGAG AGGGAAGAGA AGCGGAAGAA AAGGCAGGAG 
CGGGCCATGG AGCGAAAGAG GAAGCTAAAA GAAAAGGAAA AAAAGGAAGG GAAACATGGA 
GAATCGCTGG GTGGCGTTTT ACTCAGCGAT AATGACAAGA TGCTGCTGCA GCGCTGGGTA 
CGAATGATGG ACAAACCAGC AGACAAATCA CAAGGCATAG AAACCAGTAA TGCCAAAACC 
AGCGACAGTA ATATGGCCCA AATACAGCCA GCAACAGGAA ACAACATTAT AAAAACAAAC 
TCCAATGGGG GGGAATCAGT GCTGCCAAAA CAGCCCAACA AAGCACAGGT TTTCAAACCG 
CCACAGTTGG TGTCTCAGAT GGGACAGTGT GTCACTGCTG GACTGTTTTA TCCAACTTCA 
ACCCATGATG CATCTGTCCC TTTGGGTCAA GCTCAGAATG GAGACGTTGG TATTGTTGCA 
GGAGTTGGAA GTGTTGGAGG CTTAGGTATG GTAACAGCCC CATGTGCCTT TGCCAAAAAC 
AACATATTAA AATCACAGTT TGTTGGAACA GTGTTCACGG GTCTTGAAAA CTGGACAGGA 
ACATCTTCAC ACAAAACCCA TCAACCTCCC CCAGAACTGA ACAATCCTGG ATTTGCACAC 
ATGCCAACCC ATTCAAAGCC CAACACTCAC GTCCAGCTGT TGCCGCTGGA GCCCTTCATG 
AACAAACAGT CAGCCCTGAA CACCGGCATC AGAGTTTCAG ACACCAGCAC CAATGCAGAG 
TCCAGAGACG GTCCTCAAGC TTTGGAGAAG CTCTGCTTTT TGGGGGAGCG TCAGAATGGC 
TCTCATTCAC AAGGCTCTTC AAGGCAGCCT CCAGGTGCTG CAGTTCAACC CTGCCTGAGT 
CTCACTGACA CGGGACAGTC TTCGAACAGC ATCCCTGATA TCCACACCGT CACTCAGCAG 
CTCTCCAAAT CACAGGTTGA AGACATTTTG CCGCCAGTTT TCTCAGTGAC TCCTAAAGGC 
AGTGGAGCCG GTTATGGTGT GGGCTTTGAC CTCGATGACC TCCTCACTCA GTCTCTAACT 
GAACTCCAAC ATTCTGACCT CAGGGAAAGT CACAATGACC TAGCCCCTCT GTCTGCATCC 
CTGCTTTCTG ACTGGCTGGA GGTGCATCGC ATGACCCCTG CGGACATGGA GTCACTTCAG 
CAGGAGCTTC AACTGGGATC TCCCATGATC CTCTCAGACA ACTCTACGCT TCCTGATACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156059.1
CDS	133..3375
Translation	MASNKMDETNNSHTAGEASEGDSRRESNIEKVQQRGVTEGPAQPSNDTSTVTDDTTVADKNLALLKAHSLDVKFEVGDEYDIIETIGTGAYGVVSSARRRDNGQQVAIKKIPNAFEVVTNAKRTLRELKILKHFKHDNIIAIKDILQPVVPHSAFKSVYVVLDLMESDLHQIIHSRQPLTPEHTRYFLYQLLRGLKYIHSANVIHRDLKPSNLLVNENCELKIGDFGMARGLSAVYSEESRSFMTEYVATRWYRAPELMLSLHHYSLAIDLWSVGCIFGEMLGRRQMFPGKNYVHQLQLILSVLGTPPESIVGSIGSDRVRSYVRSLPSKAPEPLAALYPQAEPSALNLLAAMLRFDPRERISACQALEHPYLSKYHDPDDEPVCVPAFNFEFDRQPMGKEQIKEAILTEIQDFHKKKQGVRKKIQFKPLHSSGSALVGVSSNSHCTAQQSQHLKEMSQTHQQHSLNFKPADTTQMNVNSFQTPHLIGQILPHLEQSVSCQDVDMPSANSDGQPETIDLTTPTDKDTSFTEPAIDCRKDKSVLSNYTAPSRSVSQTPPTSLPQSVPSLSLSQSQVQSLSQNLSRSLGKGGKPTAAEGTKKEGAISEDTKAALKAALQKSALRQKARDEGTAALGIDLVRGASVGLSSSISSSNSCPEQRRPITAQERQREREEKRKKRQERAMERKRKLKEKEKKEGKHGESLGGVLLSDNDKMLLQRWVRMMDKPADKSQGIETSNAKTSDSNMAQIQPATGNNIIKTNSNGGESVLPKQPNKAQVFKPPQLVSQMGQCVTAGLFYPTSTHDASVPLGQAQNGDVGIVAGVGSVGGLGMVTAPCAFAKNNILKSQFVGTVFTGLENWTGTSSHKTHQPPPELNNPGFAHMPTHSKPNTHVQLLPLEPFMNKQSALNTGIRVSDTSTNAESRDGPQALEKLCFLGERQNGSHSQGSSRQPPGAAVQPCLSLTDTGQSSNSIPDIHTVTQQLSKSQVEDILPPVFSVTPKGSGAGYGVGFDLDDLLTQSLTELQHSDLRESHNDLAPLSASLLSDWLEVHRMTPADMESLQQELQLGSPMILSDNSTLPDT

Target ORF information:

RefSeq Version	XM_005156002.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mitogen-activated protein kinase 7 (mapk7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156002.4

ORF Insert Sequence:

ATGGCATCCA ATAAGATGGA TGAGACAAAT AACAGTCACA CAGCAGGAGA AGCCTCAGAA 
GGCGACAGCA GGAGAGAGAG CAACATTGAA AAGGTCCAGC AAAGAGGCGT TACAGAAGGA 
CCAGCTCAAC CCAGCAATGA TACGTCCACA GTTACTGATG ACACTACTGT CGCAGACAAG 
AACTTGGCCC TGCTCAAAGC CCACTCCCTG GATGTGAAGT TTGAAGTCGG GGATGAATAT 
GACATTATTG AGACCATTGG TACAGGTGCA TATGGTGTGG TCTCATCAGC ACGAAGACGG 
GATAATGGCC AGCAGGTGGC AATAAAGAAG ATCCCCAATG CCTTTGAGGT TGTGACGAAT 
GCCAAACGCA CGCTGCGAGA GCTCAAAATA CTGAAGCACT TCAAACACGA CAACATCATC 
GCCATTAAAG ATATTCTCCA GCCCGTGGTC CCTCATTCAG CGTTCAAATC AGTGTACGTG 
GTTCTTGACC TCATGGAGAG CGACCTACAT CAGATCATTC ACTCGCGGCA GCCTTTGACT 
CCAGAACACA CACGCTACTT TCTATATCAG CTGTTAAGAG GCTTGAAATA CATTCATTCA 
GCCAACGTCA TTCACCGCGA CCTCAAGCCA TCCAACCTGC TGGTCAATGA AAACTGCGAG 
CTAAAGATCG GGGACTTCGG AATGGCACGG GGATTAAGTG CGGTCTACTC TGAAGAGTCG 
CGGTCCTTTA TGACCGAGTA CGTGGCCACC CGCTGGTATC GTGCTCCTGA GCTCATGCTG 
TCGCTCCACC ACTACAGCCT GGCCATCGAC CTCTGGTCCG TAGGCTGCAT CTTCGGTGAG 
ATGCTCGGTA GGAGGCAGAT GTTTCCGGGG AAAAACTACG TGCATCAGTT GCAGCTGATT 
CTTTCAGTTT TAGGGACGCC ACCCGAAAGC ATTGTTGGCT CAATTGGGTC AGATAGGGTG 
CGCTCTTATG TGCGGAGCCT TCCATCCAAA GCACCAGAAC CGCTTGCAGC CCTGTATCCA 
CAAGCTGAGC CCAGCGCCTT GAACTTGTTG GCTGCAATGC TCCGCTTTGA CCCTCGTGAA 
AGAATCAGTG CATGTCAAGC ACTGGAACAC CCGTATCTCT CCAAGTACCA CGACCCAGAT 
GACGAACCGG TGTGCGTGCC TGCTTTCAAT TTTGAGTTCG ACCGGCAGCC CATGGGCAAA 
GAGCAAATCA AAGAAGCGAT ACTAACAGAG ATCCAGGACT TTCATAAGAA AAAGCAGGGC 
GTGAGGAAGA AGATCCAGTT TAAGCCGCTC CATTCTTCTG GATCGGCCTT GGTCGGTGTT 
TCTAGTAACA GCCATTGCAC AGCGCAACAG TCTCAACACC TGAAGGAGAT GTCACAAACT 
CACCAGCAGC ACAGTCTCAA CTTCAAACCA GCAGACACTA CTCAAATGAA TGTAAACTCG 
TTTCAAACTC CACATCTTAT TGGTCAAATT TTGCCACATT TGGAACAGTC GGTAAGCTGT 
CAGGATGTGG ACATGCCCAG TGCGAACTCT GACGGCCAGC CGGAAACCAT TGATCTGACC 
ACTCCCACAG ACAAGGACAC GTCTTTTACT GAGCCTGCGA TAGACTGCAG GAAGGATAAG 
AGTGTGTTAT CCAATTACAC TGCTCCATCT CGATCCGTCT CTCAGACTCC ACCCACAAGT 
CTTCCACAGT CAGTCCCTTC ACTCTCTCTC TCACAGTCCC AGGTGCAGTC GCTCTCTCAA 
AATCTCTCTC GTTCGCTTGG AAAAGGAGGA AAACCGACTG CTGCGGAAGG TACGAAAAAA 
GAGGGAGCAA TATCTGAAGA CACAAAAGCA GCTCTCAAAG CTGCCTTGCA GAAATCTGCG 
CTAAGACAGA AGGCACGAGA TGAAGGCACT GCAGCTCTTG GTATAGATTT AGTCAGAGGA 
GCTTCTGTTG GTTTATCCTC ATCCATATCC TCCTCCAACT CCTGTCCAGA GCAGAGGAGG 
CCCATCACGG CCCAGGAGAG ACAACGGGAG AGGGAAGAGA AGCGGAAGAA AAGGCAGGAG 
CGGGCCATGG AGCGAAAGAG GAAGCTAAAA GAAAAGGAAA AAAAGGAAGG GAAACATGGA 
GAATCGCTGG GTGGCGTTTT ACTCAGCGAT AATGACAAGA TGCTGCTGCA GCGCTGGGTA 
CGAATGATGG ACAAACCAGC AGACAAATCA CAAGGCATAG AAACCAGTAA TGCCAAAACC 
AGCGACAGTA ATATGGCCCA AATACAGCCA GCAACAGGAA ACAACATTAT AAAAACAAAC 
TCCAATGGGG GGGAATCAGT GCTGCCAAAA CAGCCCAACA AAGCACAGGT TTTCAAACCG 
CCACAGTTGG TGTCTCAGAT GGGACAGTGT GTCACTGCTG GACTGTTTTA TCCAACTTCA 
ACCCATGATG CATCTGTCCC TTTGGGTCAA GCTCAGAATG GAGACGTTGG TATTGTTGCA 
GGAGTTGGAA GTGTTGGAGG CTTAGGTATG GTAACAGCCC CATGTGCCTT TGCCAAAAAC 
AACATATTAA AATCACAGTT TGTTGGAACA GTGTTCACGG GTCTTGAAAA CTGGACAGGA 
ACATCTTCAC ACAAAACCCA TCAACCTCCC CCAGAACTGA ACAATCCTGG ATTTGCACAC 
ATGCCAACCC ATTCAAAGCC CAACACTCAC GTCCAGCTGT TGCCGCTGGA GCCCTTCATG 
AACAAACAGT CAGCCCTGAA CACCGGCATC AGAGTTTCAG ACACCAGCAC CAATGCAGAG 
TCCAGAGACG GTCCTCAAGC TTTGGAGAAG CTCTGCTTTT TGGGGGAGCG TCAGAATGGC 
TCTCATTCAC AAGGCTCTTC AAGGCAGCCT CCAGGTGCTG CAGTTCAACC CTGCCTGAGT 
CTCACTGACA CGGGACAGTC TTCGAACAGC ATCCCTGATA TCCACACCGT CACTCAGCAG 
CTCTCCAAAT CACAGGTTGA AGACATTTTG CCGCCAGTTT TCTCAGTGAC TCCTAAAGGC 
AGTGGAGCCG GTTATGGTGT GGGCTTTGAC CTCGATGACC TCCTCACTCA GTCTCTAACT 
GAACTCCAAC ATTCTGACCT CAGGGAAAGT CACAATGACC TAGCCCCTCT GTCTGCATCC 
CTGCTTTCTG ACTGGCTGGA GGTGCATCGC ATGACCCCTG CGGACATGGA GTCACTTCAG 
CAGGAGCTTC AACTGGGATC TCCCATGATC CTCTCAGACA ACTCTACGCT TCCTGATACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAPK7 ( XM_005156002.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005156059.1 Danio rerio mitogen-activated protein kinase 7 (mapk7), transcript variant X1, mRNA.

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CloneID	ODa17534
Clone ID Related Accession (Same CDS sequence)	XM_005156002.4 , XM_005156002.3
Accession Version	XM_005156002.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	mitogen-activated protein kinase 7 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005156002.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCCA ATAAGATGGA TGAGACAAAT AACAGTCACA CAGCAGGAGA AGCCTCAGAA 
GGCGACAGCA GGAGAGAGAG CAACATTGAA AAGGTCCAGC AAAGAGGCGT TACAGAAGGA 
CCAGCTCAAC CCAGCAATGA TACGTCCACA GTTACTGATG ACACTACTGT CGCAGACAAG 
AACTTGGCCC TGCTCAAAGC CCACTCCCTG GATGTGAAGT TTGAAGTCGG GGATGAATAT 
GACATTATTG AGACCATTGG TACAGGTGCA TATGGTGTGG TCTCATCAGC ACGAAGACGG 
GATAATGGCC AGCAGGTGGC AATAAAGAAG ATCCCCAATG CCTTTGAGGT TGTGACGAAT 
GCCAAACGCA CGCTGCGAGA GCTCAAAATA CTGAAGCACT TCAAACACGA CAACATCATC 
GCCATTAAAG ATATTCTCCA GCCCGTGGTC CCTCATTCAG CGTTCAAATC AGTGTACGTG 
GTTCTTGACC TCATGGAGAG CGACCTACAT CAGATCATTC ACTCGCGGCA GCCTTTGACT 
CCAGAACACA CACGCTACTT TCTATATCAG CTGTTAAGAG GCTTGAAATA CATTCATTCA 
GCCAACGTCA TTCACCGCGA CCTCAAGCCA TCCAACCTGC TGGTCAATGA AAACTGCGAG 
CTAAAGATCG GGGACTTCGG AATGGCACGG GGATTAAGTG CGGTCTACTC TGAAGAGTCG 
CGGTCCTTTA TGACCGAGTA CGTGGCCACC CGCTGGTATC GTGCTCCTGA GCTCATGCTG 
TCGCTCCACC ACTACAGCCT GGCCATCGAC CTCTGGTCCG TAGGCTGCAT CTTCGGTGAG 
ATGCTCGGTA GGAGGCAGAT GTTTCCGGGG AAAAACTACG TGCATCAGTT GCAGCTGATT 
CTTTCAGTTT TAGGGACGCC ACCCGAAAGC ATTGTTGGCT CAATTGGGTC AGATAGGGTG 
CGCTCTTATG TGCGGAGCCT TCCATCCAAA GCACCAGAAC CGCTTGCAGC CCTGTATCCA 
CAAGCTGAGC CCAGCGCCTT GAACTTGTTG GCTGCAATGC TCCGCTTTGA CCCTCGTGAA 
AGAATCAGTG CATGTCAAGC ACTGGAACAC CCGTATCTCT CCAAGTACCA CGACCCAGAT 
GACGAACCGG TGTGCGTGCC TGCTTTCAAT TTTGAGTTCG ACCGGCAGCC CATGGGCAAA 
GAGCAAATCA AAGAAGCGAT ACTAACAGAG ATCCAGGACT TTCATAAGAA AAAGCAGGGC 
GTGAGGAAGA AGATCCAGTT TAAGCCGCTC CATTCTTCTG GATCGGCCTT GGTCGGTGTT 
TCTAGTAACA GCCATTGCAC AGCGCAACAG TCTCAACACC TGAAGGAGAT GTCACAAACT 
CACCAGCAGC ACAGTCTCAA CTTCAAACCA GCAGACACTA CTCAAATGAA TGTAAACTCG 
TTTCAAACTC CACATCTTAT TGGTCAAATT TTGCCACATT TGGAACAGTC GGTAAGCTGT 
CAGGATGTGG ACATGCCCAG TGCGAACTCT GACGGCCAGC CGGAAACCAT TGATCTGACC 
ACTCCCACAG ACAAGGACAC GTCTTTTACT GAGCCTGCGA TAGACTGCAG GAAGGATAAG 
AGTGTGTTAT CCAATTACAC TGCTCCATCT CGATCCGTCT CTCAGACTCC ACCCACAAGT 
CTTCCACAGT CAGTCCCTTC ACTCTCTCTC TCACAGTCCC AGGTGCAGTC GCTCTCTCAA 
AATCTCTCTC GTTCGCTTGG AAAAGGAGGA AAACCGACTG CTGCGGAAGG TACGAAAAAA 
GAGGGAGCAA TATCTGAAGA CACAAAAGCA GCTCTCAAAG CTGCCTTGCA GAAATCTGCG 
CTAAGACAGA AGGCACGAGA TGAAGGCACT GCAGCTCTTG GTATAGATTT AGTCAGAGGA 
GCTTCTGTTG GTTTATCCTC ATCCATATCC TCCTCCAACT CCTGTCCAGA GCAGAGGAGG 
CCCATCACGG CCCAGGAGAG ACAACGGGAG AGGGAAGAGA AGCGGAAGAA AAGGCAGGAG 
CGGGCCATGG AGCGAAAGAG GAAGCTAAAA GAAAAGGAAA AAAAGGAAGG GAAACATGGA 
GAATCGCTGG GTGGCGTTTT ACTCAGCGAT AATGACAAGA TGCTGCTGCA GCGCTGGGTA 
CGAATGATGG ACAAACCAGC AGACAAATCA CAAGGCATAG AAACCAGTAA TGCCAAAACC 
AGCGACAGTA ATATGGCCCA AATACAGCCA GCAACAGGAA ACAACATTAT AAAAACAAAC 
TCCAATGGGG GGGAATCAGT GCTGCCAAAA CAGCCCAACA AAGCACAGGT TTTCAAACCG 
CCACAGTTGG TGTCTCAGAT GGGACAGTGT GTCACTGCTG GACTGTTTTA TCCAACTTCA 
ACCCATGATG CATCTGTCCC TTTGGGTCAA GCTCAGAATG GAGACGTTGG TATTGTTGCA 
GGAGTTGGAA GTGTTGGAGG CTTAGGTATG GTAACAGCCC CATGTGCCTT TGCCAAAAAC 
AACATATTAA AATCACAGTT TGTTGGAACA GTGTTCACGG GTCTTGAAAA CTGGACAGGA 
ACATCTTCAC ACAAAACCCA TCAACCTCCC CCAGAACTGA ACAATCCTGG ATTTGCACAC 
ATGCCAACCC ATTCAAAGCC CAACACTCAC GTCCAGCTGT TGCCGCTGGA GCCCTTCATG 
AACAAACAGT CAGCCCTGAA CACCGGCATC AGAGTTTCAG ACACCAGCAC CAATGCAGAG 
TCCAGAGACG GTCCTCAAGC TTTGGAGAAG CTCTGCTTTT TGGGGGAGCG TCAGAATGGC 
TCTCATTCAC AAGGCTCTTC AAGGCAGCCT CCAGGTGCTG CAGTTCAACC CTGCCTGAGT 
CTCACTGACA CGGGACAGTC TTCGAACAGC ATCCCTGATA TCCACACCGT CACTCAGCAG 
CTCTCCAAAT CACAGGTTGA AGACATTTTG CCGCCAGTTT TCTCAGTGAC TCCTAAAGGC 
AGTGGAGCCG GTTATGGTGT GGGCTTTGAC CTCGATGACC TCCTCACTCA GTCTCTAACT 
GAACTCCAAC ATTCTGACCT CAGGGAAAGT CACAATGACC TAGCCCCTCT GTCTGCATCC 
CTGCTTTCTG ACTGGCTGGA GGTGCATCGC ATGACCCCTG CGGACATGGA GTCACTTCAG 
CAGGAGCTTC AACTGGGATC TCCCATGATC CTCTCAGACA ACTCTACGCT TCCTGATACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156059.1
CDS	114..3356
Translation	MASNKMDETNNSHTAGEASEGDSRRESNIEKVQQRGVTEGPAQPSNDTSTVTDDTTVADKNLALLKAHSLDVKFEVGDEYDIIETIGTGAYGVVSSARRRDNGQQVAIKKIPNAFEVVTNAKRTLRELKILKHFKHDNIIAIKDILQPVVPHSAFKSVYVVLDLMESDLHQIIHSRQPLTPEHTRYFLYQLLRGLKYIHSANVIHRDLKPSNLLVNENCELKIGDFGMARGLSAVYSEESRSFMTEYVATRWYRAPELMLSLHHYSLAIDLWSVGCIFGEMLGRRQMFPGKNYVHQLQLILSVLGTPPESIVGSIGSDRVRSYVRSLPSKAPEPLAALYPQAEPSALNLLAAMLRFDPRERISACQALEHPYLSKYHDPDDEPVCVPAFNFEFDRQPMGKEQIKEAILTEIQDFHKKKQGVRKKIQFKPLHSSGSALVGVSSNSHCTAQQSQHLKEMSQTHQQHSLNFKPADTTQMNVNSFQTPHLIGQILPHLEQSVSCQDVDMPSANSDGQPETIDLTTPTDKDTSFTEPAIDCRKDKSVLSNYTAPSRSVSQTPPTSLPQSVPSLSLSQSQVQSLSQNLSRSLGKGGKPTAAEGTKKEGAISEDTKAALKAALQKSALRQKARDEGTAALGIDLVRGASVGLSSSISSSNSCPEQRRPITAQERQREREEKRKKRQERAMERKRKLKEKEKKEGKHGESLGGVLLSDNDKMLLQRWVRMMDKPADKSQGIETSNAKTSDSNMAQIQPATGNNIIKTNSNGGESVLPKQPNKAQVFKPPQLVSQMGQCVTAGLFYPTSTHDASVPLGQAQNGDVGIVAGVGSVGGLGMVTAPCAFAKNNILKSQFVGTVFTGLENWTGTSSHKTHQPPPELNNPGFAHMPTHSKPNTHVQLLPLEPFMNKQSALNTGIRVSDTSTNAESRDGPQALEKLCFLGERQNGSHSQGSSRQPPGAAVQPCLSLTDTGQSSNSIPDIHTVTQQLSKSQVEDILPPVFSVTPKGSGAGYGVGFDLDDLLTQSLTELQHSDLRESHNDLAPLSASLLSDWLEVHRMTPADMESLQQELQLGSPMILSDNSTLPDT

Target ORF information:

RefSeq Version	XM_005156002.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mitogen-activated protein kinase 7 (mapk7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156002.3

ORF Insert Sequence:

ATGGCATCCA ATAAGATGGA TGAGACAAAT AACAGTCACA CAGCAGGAGA AGCCTCAGAA 
GGCGACAGCA GGAGAGAGAG CAACATTGAA AAGGTCCAGC AAAGAGGCGT TACAGAAGGA 
CCAGCTCAAC CCAGCAATGA TACGTCCACA GTTACTGATG ACACTACTGT CGCAGACAAG 
AACTTGGCCC TGCTCAAAGC CCACTCCCTG GATGTGAAGT TTGAAGTCGG GGATGAATAT 
GACATTATTG AGACCATTGG TACAGGTGCA TATGGTGTGG TCTCATCAGC ACGAAGACGG 
GATAATGGCC AGCAGGTGGC AATAAAGAAG ATCCCCAATG CCTTTGAGGT TGTGACGAAT 
GCCAAACGCA CGCTGCGAGA GCTCAAAATA CTGAAGCACT TCAAACACGA CAACATCATC 
GCCATTAAAG ATATTCTCCA GCCCGTGGTC CCTCATTCAG CGTTCAAATC AGTGTACGTG 
GTTCTTGACC TCATGGAGAG CGACCTACAT CAGATCATTC ACTCGCGGCA GCCTTTGACT 
CCAGAACACA CACGCTACTT TCTATATCAG CTGTTAAGAG GCTTGAAATA CATTCATTCA 
GCCAACGTCA TTCACCGCGA CCTCAAGCCA TCCAACCTGC TGGTCAATGA AAACTGCGAG 
CTAAAGATCG GGGACTTCGG AATGGCACGG GGATTAAGTG CGGTCTACTC TGAAGAGTCG 
CGGTCCTTTA TGACCGAGTA CGTGGCCACC CGCTGGTATC GTGCTCCTGA GCTCATGCTG 
TCGCTCCACC ACTACAGCCT GGCCATCGAC CTCTGGTCCG TAGGCTGCAT CTTCGGTGAG 
ATGCTCGGTA GGAGGCAGAT GTTTCCGGGG AAAAACTACG TGCATCAGTT GCAGCTGATT 
CTTTCAGTTT TAGGGACGCC ACCCGAAAGC ATTGTTGGCT CAATTGGGTC AGATAGGGTG 
CGCTCTTATG TGCGGAGCCT TCCATCCAAA GCACCAGAAC CGCTTGCAGC CCTGTATCCA 
CAAGCTGAGC CCAGCGCCTT GAACTTGTTG GCTGCAATGC TCCGCTTTGA CCCTCGTGAA 
AGAATCAGTG CATGTCAAGC ACTGGAACAC CCGTATCTCT CCAAGTACCA CGACCCAGAT 
GACGAACCGG TGTGCGTGCC TGCTTTCAAT TTTGAGTTCG ACCGGCAGCC CATGGGCAAA 
GAGCAAATCA AAGAAGCGAT ACTAACAGAG ATCCAGGACT TTCATAAGAA AAAGCAGGGC 
GTGAGGAAGA AGATCCAGTT TAAGCCGCTC CATTCTTCTG GATCGGCCTT GGTCGGTGTT 
TCTAGTAACA GCCATTGCAC AGCGCAACAG TCTCAACACC TGAAGGAGAT GTCACAAACT 
CACCAGCAGC ACAGTCTCAA CTTCAAACCA GCAGACACTA CTCAAATGAA TGTAAACTCG 
TTTCAAACTC CACATCTTAT TGGTCAAATT TTGCCACATT TGGAACAGTC GGTAAGCTGT 
CAGGATGTGG ACATGCCCAG TGCGAACTCT GACGGCCAGC CGGAAACCAT TGATCTGACC 
ACTCCCACAG ACAAGGACAC GTCTTTTACT GAGCCTGCGA TAGACTGCAG GAAGGATAAG 
AGTGTGTTAT CCAATTACAC TGCTCCATCT CGATCCGTCT CTCAGACTCC ACCCACAAGT 
CTTCCACAGT CAGTCCCTTC ACTCTCTCTC TCACAGTCCC AGGTGCAGTC GCTCTCTCAA 
AATCTCTCTC GTTCGCTTGG AAAAGGAGGA AAACCGACTG CTGCGGAAGG TACGAAAAAA 
GAGGGAGCAA TATCTGAAGA CACAAAAGCA GCTCTCAAAG CTGCCTTGCA GAAATCTGCG 
CTAAGACAGA AGGCACGAGA TGAAGGCACT GCAGCTCTTG GTATAGATTT AGTCAGAGGA 
GCTTCTGTTG GTTTATCCTC ATCCATATCC TCCTCCAACT CCTGTCCAGA GCAGAGGAGG 
CCCATCACGG CCCAGGAGAG ACAACGGGAG AGGGAAGAGA AGCGGAAGAA AAGGCAGGAG 
CGGGCCATGG AGCGAAAGAG GAAGCTAAAA GAAAAGGAAA AAAAGGAAGG GAAACATGGA 
GAATCGCTGG GTGGCGTTTT ACTCAGCGAT AATGACAAGA TGCTGCTGCA GCGCTGGGTA 
CGAATGATGG ACAAACCAGC AGACAAATCA CAAGGCATAG AAACCAGTAA TGCCAAAACC 
AGCGACAGTA ATATGGCCCA AATACAGCCA GCAACAGGAA ACAACATTAT AAAAACAAAC 
TCCAATGGGG GGGAATCAGT GCTGCCAAAA CAGCCCAACA AAGCACAGGT TTTCAAACCG 
CCACAGTTGG TGTCTCAGAT GGGACAGTGT GTCACTGCTG GACTGTTTTA TCCAACTTCA 
ACCCATGATG CATCTGTCCC TTTGGGTCAA GCTCAGAATG GAGACGTTGG TATTGTTGCA 
GGAGTTGGAA GTGTTGGAGG CTTAGGTATG GTAACAGCCC CATGTGCCTT TGCCAAAAAC 
AACATATTAA AATCACAGTT TGTTGGAACA GTGTTCACGG GTCTTGAAAA CTGGACAGGA 
ACATCTTCAC ACAAAACCCA TCAACCTCCC CCAGAACTGA ACAATCCTGG ATTTGCACAC 
ATGCCAACCC ATTCAAAGCC CAACACTCAC GTCCAGCTGT TGCCGCTGGA GCCCTTCATG 
AACAAACAGT CAGCCCTGAA CACCGGCATC AGAGTTTCAG ACACCAGCAC CAATGCAGAG 
TCCAGAGACG GTCCTCAAGC TTTGGAGAAG CTCTGCTTTT TGGGGGAGCG TCAGAATGGC 
TCTCATTCAC AAGGCTCTTC AAGGCAGCCT CCAGGTGCTG CAGTTCAACC CTGCCTGAGT 
CTCACTGACA CGGGACAGTC TTCGAACAGC ATCCCTGATA TCCACACCGT CACTCAGCAG 
CTCTCCAAAT CACAGGTTGA AGACATTTTG CCGCCAGTTT TCTCAGTGAC TCCTAAAGGC 
AGTGGAGCCG GTTATGGTGT GGGCTTTGAC CTCGATGACC TCCTCACTCA GTCTCTAACT 
GAACTCCAAC ATTCTGACCT CAGGGAAAGT CACAATGACC TAGCCCCTCT GTCTGCATCC 
CTGCTTTCTG ACTGGCTGGA GGTGCATCGC ATGACCCCTG CGGACATGGA GTCACTTCAG 
CAGGAGCTTC AACTGGGATC TCCCATGATC CTCTCAGACA ACTCTACGCT TCCTGATACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Progress of CRISPR/Cas9-Mediated Gene Editing in Generating Mouse/Zebrafish Models of Human Skeletal Diseases.
Computational and structural biotechnology journal17954-962(2019)
Wu N,Liu B,Du H,Zhao S,Li Y,Cheng X,Wang S,Lin J,Zhou J,,Qiu G,Wu Z,Zhang J

The discrepancy function of NLRC5 isoforms in antiviral and antibacterial immune responses.
Developmental and comparative immunology84153-163(2018 07)
Cao L,Wu XM,Hu YW,Xue NN,Nie P,Chang MX

Mutant MAPK7-Induced Idiopathic Scoliosis is Linked to Impaired Osteogenesis.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology48(3)880-890(2018)
Zhou T,Chen C,Xu C,Zhou H,Gao B,Su D,Liao Z,Li Y,Yang S,Su P

Blood Flow Regulates Zebrafish Caudal Vein Plexus Angiogenesis by ERK5-klf2a-nos2b Signaling.
Current molecular medicine18(1)3-14(2018)
Xie X,Zhou T,Wang Y,Chen H,Lei D,Huang L,Wang Y,Jin X,Sun T,Tan J,Yin T,Huang J,Gregersen H,Wang G

Rare coding variants in MAPK7 predispose to adolescent idiopathic scoliosis.
Human mutation38(11)1500-1510(2017 11)
Gao W,Chen C,Zhou T,Yang S,Gao B,Zhou H,Lian C,Wu Z,Qiu X,Yang X,Alattar E,Liu W,Su D,Sun S,Chen Y,Cheung KMC,Song Y,Luk KKD,Chan D,Sham PC,Xing C,Khor CC,Liu G,Yang J,Deng Y,Hao D,Huang D,Li QZ,Xu C,Su P

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MAPK7 cDNA ORF clone, Danio rerio(Zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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