unc5b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	unc5b
Entrez Gene ID	494130
Full Name	unc-5 netrin receptor B
Synonyms	sb:eu495,unc5h2
General protein information	Preferred Names unc-5 netrin receptor B Names netrin receptor UNC5B unc-5 homolog B
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009307312.2	XP_009305587.1	netrin receptor UNC5B isoform X1
NM_001111149.1	NP_001104619.1	netrin receptor UNC5B precursor
XM_009307312.3	XP_009305587.1	netrin receptor UNC5B isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	UNC5B	NP_734465.2
Macaca mulatta (Rhesus monkey)	UNC5B	XP_001106162.2
Gallus gallus (chicken)	UNC5B	NP_001026396.2
Caenorhabditis elegans (roundworm)	unc-5	NP_500823.1
Pan troglodytes (chimpanzee)	UNC5B	XP_001135844.2
Mus musculus (house mouse)	Unc5b	NP_084046.2
Rattus norvegicus (Norway rat)	Unc5b	NP_071543.1
Danio rerio (zebrafish)	unc5b	NP_001104619.1
Xenopus tropicalis (tropical clawed frog)	unc5b	XP_004916000.1
Canis lupus familiaris (dog)	UNC5B	XP_850428.1
Bos taurus (cattle)	UNC5B	NP_001092499.1

Gene Ontology
Bibliography

Related articles in PubMed

Dynamic Editome of Zebrafish under Aminoglycosides Treatment and Its Potential Involvement in Ototoxicity.
Yan S, Lu Y, He L, Zhao X, Wu L, Zhu H, Jiang M, Su Y, Cao W, Tian W, Xing Q
Frontiers in pharmacology8854(2017)

Neuronal sFlt1 and Vegfaa determine venous sprouting and spinal cord vascularization.
Wild R, Klems A, Takamiya M, Hayashi Y, Strähle U, Ando K, Mochizuki N, van Impel A, Schulte-Merker S, Krueger J, Preau L, le Noble F
Nature communications813991(2017 01)

Transcriptomic, proteomic, and metabolomic landscape of positional memory in the caudal fin of zebrafish.
Rabinowitz JS, Robitaille AM, Wang Y, Ray CA, Thummel R, Gu H, Djukovic D, Raftery D, Berndt JD, Moon RT
Proceedings of the National Academy of Sciences of the United States of America114(5)E717-E726(2017 01)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

A Floor-Plate Extracellular Protein-Protein Interaction Screen Identifies Draxin as a Secreted Netrin-1 Antagonist.
Gao X, Metzger U, Panza P, Mahalwar P, Alsheimer S, Geiger H, Maischein HM, Levesque MP, Templin M, Söllner C
Cell reports12(4)694-708(2015 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following unc5b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the unc5b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa35826	NM_001111149.1 Latest version!	Danio rerio unc-5 netrin receptor B (unc5b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa41438	XM_009307312.3 Latest version!	Danio rerio unc-5 netrin receptor B (unc5b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
ODa41438	XM_009307312.2	Danio rerio unc-5 netrin receptor B (unc5b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

unc5b ( NM_001111149.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001104619.1 Danio rerio unc-5 netrin receptor B (unc5b), mRNA.

Price & Availability↑

CloneID	ODa35826
Clone ID Related Accession (Same CDS sequence)	NM_001111149.1
Accession Version	NM_001111149.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2823bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1542902400000
Organism	Danio rerio(zebrafish)
Product	netrin receptor UNC5B precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY947468.1. On or before Dec 12, 2007 this sequence version replaced XM_001331755.1, XM_001331845.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY947468.1, BC163497.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168447, SAMEA4476732 [ECO:0000348] ##Evidence-Data-END##

ATGCTCTCAA CGTGTATACA CCGCGACCAG TCTCCCGCCT CCCTGCTCGG TTTACTTCTA 
CTCAGCTCAA CCTTAGTGAT ACATGCAGAT GGCAGCGATT ACAGTGAAGT TCTCCCGGAC 
TCGTTTCCAT CAGCTCCTGC CGAACCGCTG CCAGAATTCC AGAGTGAGCC CGAGGATGCA 
TTCATAGTAA AAAACAGACC CGTCAAACTG TCCTGCAAGG CCGCCCCTGC CACACAGATA 
TACTTTAAAT GCAATGGAGA ATGGGTGAAC CAGAATGACC ATGTGACCAA GGAAAGTCTG 
GACCATATCA CAGGTCTGGT AGTGAGAGAA GTAGATATCT CTGTCTCCCG GACGCAGGTA 
GAGGAGTTGT TTGGGCTGGA AGATTATTGG TGCCAGTGTG TTGCCTGGAG CTCGGCAGGC 
ACCACAAAGA GCCGTCGGGC TTACGTCCGC ATCGCCTACT TGAGAAAGAA CTTTGAGCAG 
GAGCCGCTTG GCAGGGAGGT GCGTCTGGAG CAGGAAGTAT TACTGCAGTG CCGTCCACCA 
GAGGGCAGCC CGCCTGCTGA GGTGGACTGG CTAAAGAACG AAGAGCTCAT TGACCCGGCG 
CTGGATTCTA ACTTTCTAAT TACCATCGAG CACGACCTGA TCATCAAACA GGCTCGACTC 
TCTGACACTG CCAACTACAC CTGTGTGGCA CGTAATGTGG TCGCTAAGAG ACGCAGCAGC 
ACTGCCACTC TCATCGTCTA CGTAAGTGGA GGCTGGTCAT CCTGGACAGA GTGGTCAGAA 
TGTAATGCTC AGTGTGGGCG GGGCTGGCAG AGACGGACAC GCAGCTGCAC CAATCCAGCA 
CCACTCAATG GAGGAGCCTT CTGTGATGGA CCGCCCTTCC AGAGAGTCAC CTGTACCACC 
CTCTGTCCAG TGGATGGAGG CTGGACCGAG TGGGCCAAGT GGTCTGCGTG TGGGACGGAG 
TGCACACATT GGCGCAGTCG GGAATGTCAG GCTCCACCGC CACGTAATGG AGGACGACAC 
TGCAGCGGCA GCATGATGGA GAGCAAGAAC TGCACTGAGG GATTATGTGC ACGCAATAAA 
AAGGTTTCTG TTGAACATAC AAGCCATCCT CTGGGCTCTG GGACTGGTGT CGCGGTGTAC 
GCAGGGCTCG TGGGAGCTCT GCTTCTCTGT GTGATCCTGG TGTTGTGTGT GGGGATTCTG 
GTCTATCGCC GGAGCTGTCG CCATCTTCAC GGTGAAATCA CAGATTCGTC ATCAGCCCTC 
ACTGCTGCCT TCCACCCCGG CAACTACAAA CCTCCACGAC AGGATAATCC ACACCTCCTG 
CATCCAACAG CTCCGCCTGA CCTCACAGCC AGTGCCGGAA CCTTCCGCGG GCCACTTTTC 
TCCTTACAAC AGGCTACACT GGACTCGCAC CACAAGATCC CCATGACCAC GTCTCCTTTG 
CTTGATCCTC TTCCCAGCCT GAAGATAAAG GTGTACAACT CCTCTACCAT GTCCTCCTTG 
GAGCTGCCGA CCGGCCTGGG GTCTGTTGAC GGGGAGATCA TGAGCTTGAA GACTGTTGGC 
TCTGGACCTA AAGACCACCA TGGCCACACG CTGCCCAGGG AGCCCAGTCA CAGCGCCAGT 
GCCACGCTGG GCTCACTGGG TGGCCGTCTC ACCATCCCTA ATACAGGGGT GGGCCTGCTG 
GTTCCACCCG GGACGATTCC TCAGGGCAAG TTCTACGAGA TGTACCTCAT TATCAACAAA 
TGGGAAAAAA CAACGTTGCC ATCAGATGGT AGTCAGACAG TATTAAGTCC GGTGGTGAGC 
TGCGGACCCT CAGGCATGTT GTTGAGCAGA CCTGTGGTCC TCACTCTGCC CCACTGCGCA 
CAGCTGGAGC CCCCAGACTG GACCCTCACC CTCAAAATGC AGAACCATCA AGGTGCTTGG 
GAGGAGGTGC TGACTGTGGG AGAGGAGAGT CTATCGTCAC CCTGTTATCT GCAGGTGGAG 
GAGCAGAGTT GTCATATCCT GATGGAGCAG CTGGGCACTT ATGGGCTGGT GGGACAGTCG 
GCTCCTCCTC GGCCGGCCTG TAAGAGACTG CAGTTGGCCC TGTTTGCCCC TCGAGCCCCG 
TGCCTCTCTC TGGACTACAG TCTGAGGGTT TACTGCATAC AGGACACCCC ACATGCCCTC 
AAGGAAGTAT TAGAGGTTGA GAGAAGTCTG GGTGGGGTTT TACTGGAGGA TCCCAAACCT 
TTGCTTTTTA AGGACAGTTA CCACAATCTG CGGCTGTCAA TCCATGACAT CCCTCATTCA 
CACTGGAGGA GCAAACTCCT GGCTAAGTAT CAGGAAATCC CATTCTATCA CATTTGGAGT 
GGGAGTCAGC GGCCGTTGCA CTGCACCTTC AGTCTGGAGA GAGGAAGCCT GGTGGTTTCC 
CAGCTCACCT GCAAGATCTG TGTCAGACAG GTGGAGGGAG AGGGACAGAT ATTCCAACTC 
AACACTGACA TCCAAGAGAC TTTGCCGCCC CATTCACCAT TGCCAGCAGG CGGTTCATGT 
CTGCCTTCGT CTCAAGTGGG CCCATATGCT TTCAGGCTGC CCGTCTCCAT ACGCCAGAAA 
ATTTGTGCCA GTCTGGATGC CCCCAGTGCT CGTGGCTGTG ACTGGAGAAT GCTGGCTCGC 
AGCCTGGCCT TCGACAGATA TCTGAACTAC TTTGCAACCA AACCGAGCCC CACAGGTGTG 
CTGCTGGACC TGTGGGAAGC CTGCCATCAG GGGGACGCAG ACCTGGTCTC GCTGGCGACA 
GCTCTGGAAG AGATGGGCAA GAGTGAGGTC TTGGTTGTCA TGACGACAGA TGGCGATTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001104619.1
CDS	250..3072
Translation	MLSTCIHRDQSPASLLGLLLLSSTLVIHADGSDYSEVLPDSFPSAPAEPLPEFQSEPEDAFIVKNRPVKLSCKAAPATQIYFKCNGEWVNQNDHVTKESLDHITGLVVREVDISVSRTQVEELFGLEDYWCQCVAWSSAGTTKSRRAYVRIAYLRKNFEQEPLGREVRLEQEVLLQCRPPEGSPPAEVDWLKNEELIDPALDSNFLITIEHDLIIKQARLSDTANYTCVARNVVAKRRSSTATLIVYVSGGWSSWTEWSECNAQCGRGWQRRTRSCTNPAPLNGGAFCDGPPFQRVTCTTLCPVDGGWTEWAKWSACGTECTHWRSRECQAPPPRNGGRHCSGSMMESKNCTEGLCARNKKVSVEHTSHPLGSGTGVAVYAGLVGALLLCVILVLCVGILVYRRSCRHLHGEITDSSSALTAAFHPGNYKPPRQDNPHLLHPTAPPDLTASAGTFRGPLFSLQQATLDSHHKIPMTTSPLLDPLPSLKIKVYNSSTMSSLELPTGLGSVDGEIMSLKTVGSGPKDHHGHTLPREPSHSASATLGSLGGRLTIPNTGVGLLVPPGTIPQGKFYEMYLIINKWEKTTLPSDGSQTVLSPVVSCGPSGMLLSRPVVLTLPHCAQLEPPDWTLTLKMQNHQGAWEEVLTVGEESLSSPCYLQVEEQSCHILMEQLGTYGLVGQSAPPRPACKRLQLALFAPRAPCLSLDYSLRVYCIQDTPHALKEVLEVERSLGGVLLEDPKPLLFKDSYHNLRLSIHDIPHSHWRSKLLAKYQEIPFYHIWSGSQRPLHCTFSLERGSLVVSQLTCKICVRQVEGEGQIFQLNTDIQETLPPHSPLPAGGSCLPSSQVGPYAFRLPVSIRQKICASLDAPSARGCDWRMLARSLAFDRYLNYFATKPSPTGVLLDLWEACHQGDADLVSLATALEEMGKSEVLVVMTTDGDC

Target ORF information:

RefSeq Version	NM_001111149.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio unc-5 netrin receptor B (unc5b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001111149.1

ORF Insert Sequence:

ATGCTCTCAA CGTGTATACA CCGCGACCAG TCTCCCGCCT CCCTGCTCGG TTTACTTCTA 
CTCAGCTCAA CCTTAGTGAT ACATGCAGAT GGCAGCGATT ACAGTGAAGT TCTCCCGGAC 
TCGTTTCCAT CAGCTCCTGC CGAACCGCTG CCAGAATTCC AGAGTGAGCC CGAGGATGCA 
TTCATAGTAA AAAACAGACC CGTCAAACTG TCCTGCAAGG CCGCCCCTGC CACACAGATA 
TACTTTAAAT GCAATGGAGA ATGGGTGAAC CAGAATGACC ATGTGACCAA GGAAAGTCTG 
GACCATATCA CAGGTCTGGT AGTGAGAGAA GTAGATATCT CTGTCTCCCG GACGCAGGTA 
GAGGAGTTGT TTGGGCTGGA AGATTATTGG TGCCAGTGTG TTGCCTGGAG CTCGGCAGGC 
ACCACAAAGA GCCGTCGGGC TTACGTCCGC ATCGCCTACT TGAGAAAGAA CTTTGAGCAG 
GAGCCGCTTG GCAGGGAGGT GCGTCTGGAG CAGGAAGTAT TACTGCAGTG CCGTCCACCA 
GAGGGCAGCC CGCCTGCTGA GGTGGACTGG CTAAAGAACG AAGAGCTCAT TGACCCGGCG 
CTGGATTCTA ACTTTCTAAT TACCATCGAG CACGACCTGA TCATCAAACA GGCTCGACTC 
TCTGACACTG CCAACTACAC CTGTGTGGCA CGTAATGTGG TCGCTAAGAG ACGCAGCAGC 
ACTGCCACTC TCATCGTCTA CGTAAGTGGA GGCTGGTCAT CCTGGACAGA GTGGTCAGAA 
TGTAATGCTC AGTGTGGGCG GGGCTGGCAG AGACGGACAC GCAGCTGCAC CAATCCAGCA 
CCACTCAATG GAGGAGCCTT CTGTGATGGA CCGCCCTTCC AGAGAGTCAC CTGTACCACC 
CTCTGTCCAG TGGATGGAGG CTGGACCGAG TGGGCCAAGT GGTCTGCGTG TGGGACGGAG 
TGCACACATT GGCGCAGTCG GGAATGTCAG GCTCCACCGC CACGTAATGG AGGACGACAC 
TGCAGCGGCA GCATGATGGA GAGCAAGAAC TGCACTGAGG GATTATGTGC ACGCAATAAA 
AAGGTTTCTG TTGAACATAC AAGCCATCCT CTGGGCTCTG GGACTGGTGT CGCGGTGTAC 
GCAGGGCTCG TGGGAGCTCT GCTTCTCTGT GTGATCCTGG TGTTGTGTGT GGGGATTCTG 
GTCTATCGCC GGAGCTGTCG CCATCTTCAC GGTGAAATCA CAGATTCGTC ATCAGCCCTC 
ACTGCTGCCT TCCACCCCGG CAACTACAAA CCTCCACGAC AGGATAATCC ACACCTCCTG 
CATCCAACAG CTCCGCCTGA CCTCACAGCC AGTGCCGGAA CCTTCCGCGG GCCACTTTTC 
TCCTTACAAC AGGCTACACT GGACTCGCAC CACAAGATCC CCATGACCAC GTCTCCTTTG 
CTTGATCCTC TTCCCAGCCT GAAGATAAAG GTGTACAACT CCTCTACCAT GTCCTCCTTG 
GAGCTGCCGA CCGGCCTGGG GTCTGTTGAC GGGGAGATCA TGAGCTTGAA GACTGTTGGC 
TCTGGACCTA AAGACCACCA TGGCCACACG CTGCCCAGGG AGCCCAGTCA CAGCGCCAGT 
GCCACGCTGG GCTCACTGGG TGGCCGTCTC ACCATCCCTA ATACAGGGGT GGGCCTGCTG 
GTTCCACCCG GGACGATTCC TCAGGGCAAG TTCTACGAGA TGTACCTCAT TATCAACAAA 
TGGGAAAAAA CAACGTTGCC ATCAGATGGT AGTCAGACAG TATTAAGTCC GGTGGTGAGC 
TGCGGACCCT CAGGCATGTT GTTGAGCAGA CCTGTGGTCC TCACTCTGCC CCACTGCGCA 
CAGCTGGAGC CCCCAGACTG GACCCTCACC CTCAAAATGC AGAACCATCA AGGTGCTTGG 
GAGGAGGTGC TGACTGTGGG AGAGGAGAGT CTATCGTCAC CCTGTTATCT GCAGGTGGAG 
GAGCAGAGTT GTCATATCCT GATGGAGCAG CTGGGCACTT ATGGGCTGGT GGGACAGTCG 
GCTCCTCCTC GGCCGGCCTG TAAGAGACTG CAGTTGGCCC TGTTTGCCCC TCGAGCCCCG 
TGCCTCTCTC TGGACTACAG TCTGAGGGTT TACTGCATAC AGGACACCCC ACATGCCCTC 
AAGGAAGTAT TAGAGGTTGA GAGAAGTCTG GGTGGGGTTT TACTGGAGGA TCCCAAACCT 
TTGCTTTTTA AGGACAGTTA CCACAATCTG CGGCTGTCAA TCCATGACAT CCCTCATTCA 
CACTGGAGGA GCAAACTCCT GGCTAAGTAT CAGGAAATCC CATTCTATCA CATTTGGAGT 
GGGAGTCAGC GGCCGTTGCA CTGCACCTTC AGTCTGGAGA GAGGAAGCCT GGTGGTTTCC 
CAGCTCACCT GCAAGATCTG TGTCAGACAG GTGGAGGGAG AGGGACAGAT ATTCCAACTC 
AACACTGACA TCCAAGAGAC TTTGCCGCCC CATTCACCAT TGCCAGCAGG CGGTTCATGT 
CTGCCTTCGT CTCAAGTGGG CCCATATGCT TTCAGGCTGC CCGTCTCCAT ACGCCAGAAA 
ATTTGTGCCA GTCTGGATGC CCCCAGTGCT CGTGGCTGTG ACTGGAGAAT GCTGGCTCGC 
AGCCTGGCCT TCGACAGATA TCTGAACTAC TTTGCAACCA AACCGAGCCC CACAGGTGTG 
CTGCTGGACC TGTGGGAAGC CTGCCATCAG GGGGACGCAG ACCTGGTCTC GCTGGCGACA 
GCTCTGGAAG AGATGGGCAA GAGTGAGGTC TTGGTTGTCA TGACGACAGA TGGCGATTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc5b ( XM_009307312.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009305587.1 Danio rerio unc-5 netrin receptor B (unc5b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa41438
Clone ID Related Accession (Same CDS sequence)	XM_009307312.2 , XM_009307312.3
Accession Version	XM_009307312.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2790bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	netrin receptor UNC5B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307312.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTCAA CGTGGATACA CCGCGACCAG TCTCCCGCCT CCCTGCTCGG TTTACTTCTA 
CTCAGCTCAA CCTTAGTAAT ACATGCAGAT GGCAGCGATT ACAGTGAAGT TCTCCCGGAC 
TCGTTTCCAT CAGCTCCTGC CGAACCGCTG CCAGAATTCC AGAGTGAGCC CGAGGATGCA 
TTCATAGTAA AAAACAGACC CGTCAAACTG TCCTGCAAGG CCGCCCCTGC CACACAGATA 
TACTTTAAAT GCAATGGAGA ATGGGTGAAC CAGAATGACC ATGTGACCAA GGAAAGTCTG 
GACCATATCA CAGGTCTGGT AGTGAGAGAA GTAGATATCT CTGTCTCCCG GACGCAGGTA 
GAGGAGTTGT TTGGGCTGGA GGATTATTGG TGCCAGTGTG TTGCCTGGAG CTCGGCAGGC 
ACCACAAAGA GCCGTCGGGC TTACGTCCGC ATCGCCTACT TGAGAAAGAA CTTTGAGCAG 
GAGCCGCTTG GCAGGGAGGT GCGTCTGGAG CAGGAAGTAT TACTGCAGTG TCGTCCACCA 
GAGGGCAGCC CGCCTGCTGA GGTGGACTGG CTAAAGAACG AAGAGCTCAT TGACCCGGCG 
CTGGATTCTA ACTTTCTAAT TACCATCGAG CACGACCTGA TCATCAAACA GGCTCGACTC 
TCTGACACTG CCAACTACAC CTGTGTGGCA CGTAATGTGG TCGCTAAGAG ACGCAGCAGC 
ACTGCCACTC TCATCGTCTA CGTAAGTGGA GGCTGGTCAT CCTGGACAGA GTGGTCAGAA 
TGTAATGCTC AGTGTGGGCG GGGCTGGCAG AGACGGACAC GCAGCTGCAC CAATCCAGCA 
CCACTCAATG GAGGAGCCTT CTGTGATGGA CCGCCCTTCC AGAGAGTCAC CTGTACCACC 
CTCTGTCCAG TGGATGGAGG CTGGACCGAG TGGGCCAAGT GGTCTGCGTG TGGGACGGAG 
TGCACACATT GGCGCAGTCG TGAATGTCAG GCTCCACCGC CACGTAATGG AGGACGACAC 
TGCAGCGGCA GCATGATGGA GAGCAAGAAC TGCACTGAGG GATTATGTGC ACGCACTCTG 
GGCTCTGGGA CTGGTGTCGC GGTGTACGCA GGGCTCGTGG GAGCTCTGCT TCTCTGTGTG 
ATCCTGGTGT TGTGTGTGGG GATTCTGGTC TATCGCCGGA GCTGTCGCCA TCTTCACGGT 
GAAATCACAG ATTCGTCATC AGCCCTCACT GCTGCCTTCC ACCCCGGCAA CTACAAACCT 
CCACGACAGG ATAATCCACA CCTCCTGCAT CCAACAGCTC CGCCTGACCT CACAGCCAGT 
GCCGGAACTT TCCGCGGGCC ACTTTTCTCC TTACAACAGG CTACACTGGA CTCGCACCAC 
AAGATCCCCA TGACCACGTC TCCTTTGCTT GACCCTCTTC CCAGCCTGAA GATAAAGGTG 
TACAACTCCT CTACCATGTC CTCCTTGGAG CTGCCGACCG GCCTGGGGTC TGTTGACGGG 
GATATCATGA GCTTGAAGAC TGTTGGCTCT GGACCTAAAG ATCATCATGG CCACACGCTG 
CCCAGGGAGC CCAGTCACAG CGCCAGTGCC ACGCTGGGCT CACTGGGTGG CCGTCTCACC 
ATCCCTAATA CAGGGGTAAG CCTGCTGGTT CCACCCGGGA CGATTCCTCA GGGCAAGTTC 
TACGAGATGT ACCTCATTAT CAACAAATGG GAAAAAACAA CGTTGCCATC AGATGGTAGT 
CAGACAGTAT TAAGTCCGGT GGTGAGCTGC GGACCCTCAG GCATGTTGTT GAGCAGACCT 
GTGGTCCTCA CTCTGCCCCA CTGCGCACAG CTGGAGCCCC CAGACTGGAC CCTCACCCTC 
AAAATGCAGA ACCATCAAGG TGCTTGGGAG GAGGTGCTGA CTGTGGGAGA GGAGAGTCTA 
TCGTCACCCT GTTATCTGCA GGTGGAGGAG CAGAGTTGTC ATATCCTGAT GGAGCAGCTG 
GGCACTTATG GGCTGGTGGG ACAGTCGGCT CCTCCTCGGC CGGCCTGTAA GAGACTGCAG 
TTGGCCCTGT TTGCCCCTCG GGCCCCGTGC CTCTCTCTGG ACTACAGTCT GAGGGTTTAC 
TGCATACAGG ACACCCCACA TGCCCTCAAG GAAGTATTAG AGGTTGAGAG AAGTCTGGGT 
GGGGTTTTAC TGGAGGATCC CAAACCTTTG CTTTTTAAGG ACAGTTACCA CAATATGCGG 
CTGTCGATCC ATGACATCCC TCATTCACAC TGGAGGAGCA AACTCCTGGC TAAGTATCAG 
GAAATCCCAT TCTATCACAT TTGGAGTGGG AGTCAGCGGC CGTTGCACTG CACCTTCAGT 
CTGGAGAGAG GAAGCCTGGT GGTTTCCCAG CTCACCTGCA AGATCTGTGT CAGACAGGTG 
GAGGGAGAGG GACAGATATT CCAACTCAAC ACTGACATCC AAGAGACTTT GCCGCCCCAT 
TCACCATTGC CAGCAGGCGG TTCATGTCTG CCTTCGTCTC AAGTGGGCCC ATATGCTTTC 
AGGCTGCCCG TCTCCATACG CCAGAAAATT TGTGCCAGTC TGGATGCCCC CAGTGCTCGT 
GGCTGTGACT GGAGAATGCT GGCTCGCAGC CTGGCCTTCG ACAGATATCT GAACTACTTT 
GCAACCAAAC CGAGCCCCAC AGGTGTGCTG CTGGACCTGT GGGAAGCCTG CCATCAGGGG 
GACGCAGACC TGGTCTCGCT GGCGACAGCT CTGGAAGAGA TGGGCAAGAG TGAGGTCTTG 
GTTGTCATGA CGACAGATGG CGATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305587.1
CDS	601..3390
Translation	MLSTWIHRDQSPASLLGLLLLSSTLVIHADGSDYSEVLPDSFPSAPAEPLPEFQSEPEDAFIVKNRPVKLSCKAAPATQIYFKCNGEWVNQNDHVTKESLDHITGLVVREVDISVSRTQVEELFGLEDYWCQCVAWSSAGTTKSRRAYVRIAYLRKNFEQEPLGREVRLEQEVLLQCRPPEGSPPAEVDWLKNEELIDPALDSNFLITIEHDLIIKQARLSDTANYTCVARNVVAKRRSSTATLIVYVSGGWSSWTEWSECNAQCGRGWQRRTRSCTNPAPLNGGAFCDGPPFQRVTCTTLCPVDGGWTEWAKWSACGTECTHWRSRECQAPPPRNGGRHCSGSMMESKNCTEGLCARTLGSGTGVAVYAGLVGALLLCVILVLCVGILVYRRSCRHLHGEITDSSSALTAAFHPGNYKPPRQDNPHLLHPTAPPDLTASAGTFRGPLFSLQQATLDSHHKIPMTTSPLLDPLPSLKIKVYNSSTMSSLELPTGLGSVDGDIMSLKTVGSGPKDHHGHTLPREPSHSASATLGSLGGRLTIPNTGVSLLVPPGTIPQGKFYEMYLIINKWEKTTLPSDGSQTVLSPVVSCGPSGMLLSRPVVLTLPHCAQLEPPDWTLTLKMQNHQGAWEEVLTVGEESLSSPCYLQVEEQSCHILMEQLGTYGLVGQSAPPRPACKRLQLALFAPRAPCLSLDYSLRVYCIQDTPHALKEVLEVERSLGGVLLEDPKPLLFKDSYHNMRLSIHDIPHSHWRSKLLAKYQEIPFYHIWSGSQRPLHCTFSLERGSLVVSQLTCKICVRQVEGEGQIFQLNTDIQETLPPHSPLPAGGSCLPSSQVGPYAFRLPVSIRQKICASLDAPSARGCDWRMLARSLAFDRYLNYFATKPSPTGVLLDLWEACHQGDADLVSLATALEEMGKSEVLVVMTTDGDC

Target ORF information:

RefSeq Version	XM_009307312.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio unc-5 netrin receptor B (unc5b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307312.2

ORF Insert Sequence:

ATGCTCTCAA CGTGGATACA CCGCGACCAG TCTCCCGCCT CCCTGCTCGG TTTACTTCTA 
CTCAGCTCAA CCTTAGTAAT ACATGCAGAT GGCAGCGATT ACAGTGAAGT TCTCCCGGAC 
TCGTTTCCAT CAGCTCCTGC CGAACCGCTG CCAGAATTCC AGAGTGAGCC CGAGGATGCA 
TTCATAGTAA AAAACAGACC CGTCAAACTG TCCTGCAAGG CCGCCCCTGC CACACAGATA 
TACTTTAAAT GCAATGGAGA ATGGGTGAAC CAGAATGACC ATGTGACCAA GGAAAGTCTG 
GACCATATCA CAGGTCTGGT AGTGAGAGAA GTAGATATCT CTGTCTCCCG GACGCAGGTA 
GAGGAGTTGT TTGGGCTGGA GGATTATTGG TGCCAGTGTG TTGCCTGGAG CTCGGCAGGC 
ACCACAAAGA GCCGTCGGGC TTACGTCCGC ATCGCCTACT TGAGAAAGAA CTTTGAGCAG 
GAGCCGCTTG GCAGGGAGGT GCGTCTGGAG CAGGAAGTAT TACTGCAGTG TCGTCCACCA 
GAGGGCAGCC CGCCTGCTGA GGTGGACTGG CTAAAGAACG AAGAGCTCAT TGACCCGGCG 
CTGGATTCTA ACTTTCTAAT TACCATCGAG CACGACCTGA TCATCAAACA GGCTCGACTC 
TCTGACACTG CCAACTACAC CTGTGTGGCA CGTAATGTGG TCGCTAAGAG ACGCAGCAGC 
ACTGCCACTC TCATCGTCTA CGTAAGTGGA GGCTGGTCAT CCTGGACAGA GTGGTCAGAA 
TGTAATGCTC AGTGTGGGCG GGGCTGGCAG AGACGGACAC GCAGCTGCAC CAATCCAGCA 
CCACTCAATG GAGGAGCCTT CTGTGATGGA CCGCCCTTCC AGAGAGTCAC CTGTACCACC 
CTCTGTCCAG TGGATGGAGG CTGGACCGAG TGGGCCAAGT GGTCTGCGTG TGGGACGGAG 
TGCACACATT GGCGCAGTCG TGAATGTCAG GCTCCACCGC CACGTAATGG AGGACGACAC 
TGCAGCGGCA GCATGATGGA GAGCAAGAAC TGCACTGAGG GATTATGTGC ACGCACTCTG 
GGCTCTGGGA CTGGTGTCGC GGTGTACGCA GGGCTCGTGG GAGCTCTGCT TCTCTGTGTG 
ATCCTGGTGT TGTGTGTGGG GATTCTGGTC TATCGCCGGA GCTGTCGCCA TCTTCACGGT 
GAAATCACAG ATTCGTCATC AGCCCTCACT GCTGCCTTCC ACCCCGGCAA CTACAAACCT 
CCACGACAGG ATAATCCACA CCTCCTGCAT CCAACAGCTC CGCCTGACCT CACAGCCAGT 
GCCGGAACTT TCCGCGGGCC ACTTTTCTCC TTACAACAGG CTACACTGGA CTCGCACCAC 
AAGATCCCCA TGACCACGTC TCCTTTGCTT GACCCTCTTC CCAGCCTGAA GATAAAGGTG 
TACAACTCCT CTACCATGTC CTCCTTGGAG CTGCCGACCG GCCTGGGGTC TGTTGACGGG 
GATATCATGA GCTTGAAGAC TGTTGGCTCT GGACCTAAAG ATCATCATGG CCACACGCTG 
CCCAGGGAGC CCAGTCACAG CGCCAGTGCC ACGCTGGGCT CACTGGGTGG CCGTCTCACC 
ATCCCTAATA CAGGGGTAAG CCTGCTGGTT CCACCCGGGA CGATTCCTCA GGGCAAGTTC 
TACGAGATGT ACCTCATTAT CAACAAATGG GAAAAAACAA CGTTGCCATC AGATGGTAGT 
CAGACAGTAT TAAGTCCGGT GGTGAGCTGC GGACCCTCAG GCATGTTGTT GAGCAGACCT 
GTGGTCCTCA CTCTGCCCCA CTGCGCACAG CTGGAGCCCC CAGACTGGAC CCTCACCCTC 
AAAATGCAGA ACCATCAAGG TGCTTGGGAG GAGGTGCTGA CTGTGGGAGA GGAGAGTCTA 
TCGTCACCCT GTTATCTGCA GGTGGAGGAG CAGAGTTGTC ATATCCTGAT GGAGCAGCTG 
GGCACTTATG GGCTGGTGGG ACAGTCGGCT CCTCCTCGGC CGGCCTGTAA GAGACTGCAG 
TTGGCCCTGT TTGCCCCTCG GGCCCCGTGC CTCTCTCTGG ACTACAGTCT GAGGGTTTAC 
TGCATACAGG ACACCCCACA TGCCCTCAAG GAAGTATTAG AGGTTGAGAG AAGTCTGGGT 
GGGGTTTTAC TGGAGGATCC CAAACCTTTG CTTTTTAAGG ACAGTTACCA CAATATGCGG 
CTGTCGATCC ATGACATCCC TCATTCACAC TGGAGGAGCA AACTCCTGGC TAAGTATCAG 
GAAATCCCAT TCTATCACAT TTGGAGTGGG AGTCAGCGGC CGTTGCACTG CACCTTCAGT 
CTGGAGAGAG GAAGCCTGGT GGTTTCCCAG CTCACCTGCA AGATCTGTGT CAGACAGGTG 
GAGGGAGAGG GACAGATATT CCAACTCAAC ACTGACATCC AAGAGACTTT GCCGCCCCAT 
TCACCATTGC CAGCAGGCGG TTCATGTCTG CCTTCGTCTC AAGTGGGCCC ATATGCTTTC 
AGGCTGCCCG TCTCCATACG CCAGAAAATT TGTGCCAGTC TGGATGCCCC CAGTGCTCGT 
GGCTGTGACT GGAGAATGCT GGCTCGCAGC CTGGCCTTCG ACAGATATCT GAACTACTTT 
GCAACCAAAC CGAGCCCCAC AGGTGTGCTG CTGGACCTGT GGGAAGCCTG CCATCAGGGG 
GACGCAGACC TGGTCTCGCT GGCGACAGCT CTGGAAGAGA TGGGCAAGAG TGAGGTCTTG 
GTTGTCATGA CGACAGATGG CGATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc5b ( XM_009307312.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009305587.1 Danio rerio unc-5 netrin receptor B (unc5b), transcript variant X1, mRNA.

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CloneID	ODa41438
Clone ID Related Accession (Same CDS sequence)	XM_009307312.2 , XM_009307312.3
Accession Version	XM_009307312.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2790bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	netrin receptor UNC5B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009307312.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTCAA CGTGGATACA CCGCGACCAG TCTCCCGCCT CCCTGCTCGG TTTACTTCTA 
CTCAGCTCAA CCTTAGTAAT ACATGCAGAT GGCAGCGATT ACAGTGAAGT TCTCCCGGAC 
TCGTTTCCAT CAGCTCCTGC CGAACCGCTG CCAGAATTCC AGAGTGAGCC CGAGGATGCA 
TTCATAGTAA AAAACAGACC CGTCAAACTG TCCTGCAAGG CCGCCCCTGC CACACAGATA 
TACTTTAAAT GCAATGGAGA ATGGGTGAAC CAGAATGACC ATGTGACCAA GGAAAGTCTG 
GACCATATCA CAGGTCTGGT AGTGAGAGAA GTAGATATCT CTGTCTCCCG GACGCAGGTA 
GAGGAGTTGT TTGGGCTGGA GGATTATTGG TGCCAGTGTG TTGCCTGGAG CTCGGCAGGC 
ACCACAAAGA GCCGTCGGGC TTACGTCCGC ATCGCCTACT TGAGAAAGAA CTTTGAGCAG 
GAGCCGCTTG GCAGGGAGGT GCGTCTGGAG CAGGAAGTAT TACTGCAGTG TCGTCCACCA 
GAGGGCAGCC CGCCTGCTGA GGTGGACTGG CTAAAGAACG AAGAGCTCAT TGACCCGGCG 
CTGGATTCTA ACTTTCTAAT TACCATCGAG CACGACCTGA TCATCAAACA GGCTCGACTC 
TCTGACACTG CCAACTACAC CTGTGTGGCA CGTAATGTGG TCGCTAAGAG ACGCAGCAGC 
ACTGCCACTC TCATCGTCTA CGTAAGTGGA GGCTGGTCAT CCTGGACAGA GTGGTCAGAA 
TGTAATGCTC AGTGTGGGCG GGGCTGGCAG AGACGGACAC GCAGCTGCAC CAATCCAGCA 
CCACTCAATG GAGGAGCCTT CTGTGATGGA CCGCCCTTCC AGAGAGTCAC CTGTACCACC 
CTCTGTCCAG TGGATGGAGG CTGGACCGAG TGGGCCAAGT GGTCTGCGTG TGGGACGGAG 
TGCACACATT GGCGCAGTCG TGAATGTCAG GCTCCACCGC CACGTAATGG AGGACGACAC 
TGCAGCGGCA GCATGATGGA GAGCAAGAAC TGCACTGAGG GATTATGTGC ACGCACTCTG 
GGCTCTGGGA CTGGTGTCGC GGTGTACGCA GGGCTCGTGG GAGCTCTGCT TCTCTGTGTG 
ATCCTGGTGT TGTGTGTGGG GATTCTGGTC TATCGCCGGA GCTGTCGCCA TCTTCACGGT 
GAAATCACAG ATTCGTCATC AGCCCTCACT GCTGCCTTCC ACCCCGGCAA CTACAAACCT 
CCACGACAGG ATAATCCACA CCTCCTGCAT CCAACAGCTC CGCCTGACCT CACAGCCAGT 
GCCGGAACTT TCCGCGGGCC ACTTTTCTCC TTACAACAGG CTACACTGGA CTCGCACCAC 
AAGATCCCCA TGACCACGTC TCCTTTGCTT GACCCTCTTC CCAGCCTGAA GATAAAGGTG 
TACAACTCCT CTACCATGTC CTCCTTGGAG CTGCCGACCG GCCTGGGGTC TGTTGACGGG 
GATATCATGA GCTTGAAGAC TGTTGGCTCT GGACCTAAAG ATCATCATGG CCACACGCTG 
CCCAGGGAGC CCAGTCACAG CGCCAGTGCC ACGCTGGGCT CACTGGGTGG CCGTCTCACC 
ATCCCTAATA CAGGGGTAAG CCTGCTGGTT CCACCCGGGA CGATTCCTCA GGGCAAGTTC 
TACGAGATGT ACCTCATTAT CAACAAATGG GAAAAAACAA CGTTGCCATC AGATGGTAGT 
CAGACAGTAT TAAGTCCGGT GGTGAGCTGC GGACCCTCAG GCATGTTGTT GAGCAGACCT 
GTGGTCCTCA CTCTGCCCCA CTGCGCACAG CTGGAGCCCC CAGACTGGAC CCTCACCCTC 
AAAATGCAGA ACCATCAAGG TGCTTGGGAG GAGGTGCTGA CTGTGGGAGA GGAGAGTCTA 
TCGTCACCCT GTTATCTGCA GGTGGAGGAG CAGAGTTGTC ATATCCTGAT GGAGCAGCTG 
GGCACTTATG GGCTGGTGGG ACAGTCGGCT CCTCCTCGGC CGGCCTGTAA GAGACTGCAG 
TTGGCCCTGT TTGCCCCTCG GGCCCCGTGC CTCTCTCTGG ACTACAGTCT GAGGGTTTAC 
TGCATACAGG ACACCCCACA TGCCCTCAAG GAAGTATTAG AGGTTGAGAG AAGTCTGGGT 
GGGGTTTTAC TGGAGGATCC CAAACCTTTG CTTTTTAAGG ACAGTTACCA CAATATGCGG 
CTGTCGATCC ATGACATCCC TCATTCACAC TGGAGGAGCA AACTCCTGGC TAAGTATCAG 
GAAATCCCAT TCTATCACAT TTGGAGTGGG AGTCAGCGGC CGTTGCACTG CACCTTCAGT 
CTGGAGAGAG GAAGCCTGGT GGTTTCCCAG CTCACCTGCA AGATCTGTGT CAGACAGGTG 
GAGGGAGAGG GACAGATATT CCAACTCAAC ACTGACATCC AAGAGACTTT GCCGCCCCAT 
TCACCATTGC CAGCAGGCGG TTCATGTCTG CCTTCGTCTC AAGTGGGCCC ATATGCTTTC 
AGGCTGCCCG TCTCCATACG CCAGAAAATT TGTGCCAGTC TGGATGCCCC CAGTGCTCGT 
GGCTGTGACT GGAGAATGCT GGCTCGCAGC CTGGCCTTCG ACAGATATCT GAACTACTTT 
GCAACCAAAC CGAGCCCCAC AGGTGTGCTG CTGGACCTGT GGGAAGCCTG CCATCAGGGG 
GACGCAGACC TGGTCTCGCT GGCGACAGCT CTGGAAGAGA TGGGCAAGAG TGAGGTCTTG 
GTTGTCATGA CGACAGATGG CGATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305587.1
CDS	653..3442
Translation	MLSTWIHRDQSPASLLGLLLLSSTLVIHADGSDYSEVLPDSFPSAPAEPLPEFQSEPEDAFIVKNRPVKLSCKAAPATQIYFKCNGEWVNQNDHVTKESLDHITGLVVREVDISVSRTQVEELFGLEDYWCQCVAWSSAGTTKSRRAYVRIAYLRKNFEQEPLGREVRLEQEVLLQCRPPEGSPPAEVDWLKNEELIDPALDSNFLITIEHDLIIKQARLSDTANYTCVARNVVAKRRSSTATLIVYVSGGWSSWTEWSECNAQCGRGWQRRTRSCTNPAPLNGGAFCDGPPFQRVTCTTLCPVDGGWTEWAKWSACGTECTHWRSRECQAPPPRNGGRHCSGSMMESKNCTEGLCARTLGSGTGVAVYAGLVGALLLCVILVLCVGILVYRRSCRHLHGEITDSSSALTAAFHPGNYKPPRQDNPHLLHPTAPPDLTASAGTFRGPLFSLQQATLDSHHKIPMTTSPLLDPLPSLKIKVYNSSTMSSLELPTGLGSVDGDIMSLKTVGSGPKDHHGHTLPREPSHSASATLGSLGGRLTIPNTGVSLLVPPGTIPQGKFYEMYLIINKWEKTTLPSDGSQTVLSPVVSCGPSGMLLSRPVVLTLPHCAQLEPPDWTLTLKMQNHQGAWEEVLTVGEESLSSPCYLQVEEQSCHILMEQLGTYGLVGQSAPPRPACKRLQLALFAPRAPCLSLDYSLRVYCIQDTPHALKEVLEVERSLGGVLLEDPKPLLFKDSYHNMRLSIHDIPHSHWRSKLLAKYQEIPFYHIWSGSQRPLHCTFSLERGSLVVSQLTCKICVRQVEGEGQIFQLNTDIQETLPPHSPLPAGGSCLPSSQVGPYAFRLPVSIRQKICASLDAPSARGCDWRMLARSLAFDRYLNYFATKPSPTGVLLDLWEACHQGDADLVSLATALEEMGKSEVLVVMTTDGDC

Target ORF information:

RefSeq Version	XM_009307312.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio unc-5 netrin receptor B (unc5b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307312.3

ORF Insert Sequence:

ATGCTCTCAA CGTGGATACA CCGCGACCAG TCTCCCGCCT CCCTGCTCGG TTTACTTCTA 
CTCAGCTCAA CCTTAGTAAT ACATGCAGAT GGCAGCGATT ACAGTGAAGT TCTCCCGGAC 
TCGTTTCCAT CAGCTCCTGC CGAACCGCTG CCAGAATTCC AGAGTGAGCC CGAGGATGCA 
TTCATAGTAA AAAACAGACC CGTCAAACTG TCCTGCAAGG CCGCCCCTGC CACACAGATA 
TACTTTAAAT GCAATGGAGA ATGGGTGAAC CAGAATGACC ATGTGACCAA GGAAAGTCTG 
GACCATATCA CAGGTCTGGT AGTGAGAGAA GTAGATATCT CTGTCTCCCG GACGCAGGTA 
GAGGAGTTGT TTGGGCTGGA GGATTATTGG TGCCAGTGTG TTGCCTGGAG CTCGGCAGGC 
ACCACAAAGA GCCGTCGGGC TTACGTCCGC ATCGCCTACT TGAGAAAGAA CTTTGAGCAG 
GAGCCGCTTG GCAGGGAGGT GCGTCTGGAG CAGGAAGTAT TACTGCAGTG TCGTCCACCA 
GAGGGCAGCC CGCCTGCTGA GGTGGACTGG CTAAAGAACG AAGAGCTCAT TGACCCGGCG 
CTGGATTCTA ACTTTCTAAT TACCATCGAG CACGACCTGA TCATCAAACA GGCTCGACTC 
TCTGACACTG CCAACTACAC CTGTGTGGCA CGTAATGTGG TCGCTAAGAG ACGCAGCAGC 
ACTGCCACTC TCATCGTCTA CGTAAGTGGA GGCTGGTCAT CCTGGACAGA GTGGTCAGAA 
TGTAATGCTC AGTGTGGGCG GGGCTGGCAG AGACGGACAC GCAGCTGCAC CAATCCAGCA 
CCACTCAATG GAGGAGCCTT CTGTGATGGA CCGCCCTTCC AGAGAGTCAC CTGTACCACC 
CTCTGTCCAG TGGATGGAGG CTGGACCGAG TGGGCCAAGT GGTCTGCGTG TGGGACGGAG 
TGCACACATT GGCGCAGTCG TGAATGTCAG GCTCCACCGC CACGTAATGG AGGACGACAC 
TGCAGCGGCA GCATGATGGA GAGCAAGAAC TGCACTGAGG GATTATGTGC ACGCACTCTG 
GGCTCTGGGA CTGGTGTCGC GGTGTACGCA GGGCTCGTGG GAGCTCTGCT TCTCTGTGTG 
ATCCTGGTGT TGTGTGTGGG GATTCTGGTC TATCGCCGGA GCTGTCGCCA TCTTCACGGT 
GAAATCACAG ATTCGTCATC AGCCCTCACT GCTGCCTTCC ACCCCGGCAA CTACAAACCT 
CCACGACAGG ATAATCCACA CCTCCTGCAT CCAACAGCTC CGCCTGACCT CACAGCCAGT 
GCCGGAACTT TCCGCGGGCC ACTTTTCTCC TTACAACAGG CTACACTGGA CTCGCACCAC 
AAGATCCCCA TGACCACGTC TCCTTTGCTT GACCCTCTTC CCAGCCTGAA GATAAAGGTG 
TACAACTCCT CTACCATGTC CTCCTTGGAG CTGCCGACCG GCCTGGGGTC TGTTGACGGG 
GATATCATGA GCTTGAAGAC TGTTGGCTCT GGACCTAAAG ATCATCATGG CCACACGCTG 
CCCAGGGAGC CCAGTCACAG CGCCAGTGCC ACGCTGGGCT CACTGGGTGG CCGTCTCACC 
ATCCCTAATA CAGGGGTAAG CCTGCTGGTT CCACCCGGGA CGATTCCTCA GGGCAAGTTC 
TACGAGATGT ACCTCATTAT CAACAAATGG GAAAAAACAA CGTTGCCATC AGATGGTAGT 
CAGACAGTAT TAAGTCCGGT GGTGAGCTGC GGACCCTCAG GCATGTTGTT GAGCAGACCT 
GTGGTCCTCA CTCTGCCCCA CTGCGCACAG CTGGAGCCCC CAGACTGGAC CCTCACCCTC 
AAAATGCAGA ACCATCAAGG TGCTTGGGAG GAGGTGCTGA CTGTGGGAGA GGAGAGTCTA 
TCGTCACCCT GTTATCTGCA GGTGGAGGAG CAGAGTTGTC ATATCCTGAT GGAGCAGCTG 
GGCACTTATG GGCTGGTGGG ACAGTCGGCT CCTCCTCGGC CGGCCTGTAA GAGACTGCAG 
TTGGCCCTGT TTGCCCCTCG GGCCCCGTGC CTCTCTCTGG ACTACAGTCT GAGGGTTTAC 
TGCATACAGG ACACCCCACA TGCCCTCAAG GAAGTATTAG AGGTTGAGAG AAGTCTGGGT 
GGGGTTTTAC TGGAGGATCC CAAACCTTTG CTTTTTAAGG ACAGTTACCA CAATATGCGG 
CTGTCGATCC ATGACATCCC TCATTCACAC TGGAGGAGCA AACTCCTGGC TAAGTATCAG 
GAAATCCCAT TCTATCACAT TTGGAGTGGG AGTCAGCGGC CGTTGCACTG CACCTTCAGT 
CTGGAGAGAG GAAGCCTGGT GGTTTCCCAG CTCACCTGCA AGATCTGTGT CAGACAGGTG 
GAGGGAGAGG GACAGATATT CCAACTCAAC ACTGACATCC AAGAGACTTT GCCGCCCCAT 
TCACCATTGC CAGCAGGCGG TTCATGTCTG CCTTCGTCTC AAGTGGGCCC ATATGCTTTC 
AGGCTGCCCG TCTCCATACG CCAGAAAATT TGTGCCAGTC TGGATGCCCC CAGTGCTCGT 
GGCTGTGACT GGAGAATGCT GGCTCGCAGC CTGGCCTTCG ACAGATATCT GAACTACTTT 
GCAACCAAAC CGAGCCCCAC AGGTGTGCTG CTGGACCTGT GGGAAGCCTG CCATCAGGGG 
GACGCAGACC TGGTCTCGCT GGCGACAGCT CTGGAAGAGA TGGGCAAGAG TGAGGTCTTG 
GTTGTCATGA CGACAGATGG CGATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dynamic Editome of Zebrafish under Aminoglycosides Treatment and Its Potential Involvement in Ototoxicity.
Frontiers in pharmacology8854(2017)
Yan S,Lu Y,He L,Zhao X,Wu L,Zhu H,Jiang M,Su Y,Cao W,Tian W,Xing Q

Neuronal sFlt1 and Vegfaa determine venous sprouting and spinal cord vascularization.
Nature communications813991(2017 01)
Wild R,Klems A,Takamiya M,Hayashi Y,Strähle U,Ando K,Mochizuki N,van Impel A,Schulte-Merker S,Krueger J,Preau L,le Noble F

Transcriptomic, proteomic, and metabolomic landscape of positional memory in the caudal fin of zebrafish.
Proceedings of the National Academy of Sciences of the United States of America114(5)E717-E726(2017 01)
Rabinowitz JS,Robitaille AM,Wang Y,Ray CA,Thummel R,Gu H,Djukovic D,Raftery D,Berndt JD,Moon RT

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

A Floor-Plate Extracellular Protein-Protein Interaction Screen Identifies Draxin as a Secreted Netrin-1 Antagonist.
Cell reports12(4)694-708(2015 Jul)
Gao X,Metzger U,Panza P,Mahalwar P,Alsheimer S,Geiger H,Maischein HM,Levesque MP,Templin M,Söllner C

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unc5b cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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