dhcr24 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	dhcr24
Entrez Gene ID	494102
Full Name	24-dehydrocholesterol reductase
Synonyms	zgc:101638
General protein information	Preferred Names 24-dehydrocholesterol reductase Names delta(24)-sterol reductase
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001008645.1	NP_001008645.1	delta(24)-sterol reductase

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	DHCR24	XP_546693.4
Mus musculus (house mouse)	Dhcr24	NP_444502.2
Gallus gallus (chicken)	DHCR24	NP_001026459.1
Magnaporthe oryzae (rice blast fungus)	MGG_16834	XP_003712445.1
Homo sapiens (human)	DHCR24	NP_055577.1
Xenopus tropicalis (tropical clawed frog)	dhcr24	NP_001016800.1
Macaca mulatta (Rhesus monkey)	DHCR24	XP_001108186.2
Rattus norvegicus (Norway rat)	Dhcr24	NP_001073617.1
Arabidopsis thaliana (thale cress)	DWF1	NP_850616.1
Oryza sativa (rice)	Os10g0397400	NP_001064534.1
Pan troglodytes (chimpanzee)	DHCR24	XP_001153930.1
Bos taurus (cattle)	DHCR24	NP_001096746.1
Danio rerio (zebrafish)	dhcr24	NP_001008645.1
Caenorhabditis elegans (roundworm)	F52H2.6	NP_508463.1

Gene Ontology
Bibliography

Related articles in PubMed

Role of zebrafish ClC-K/barttin channels in apical kidney chloride reabsorption.
Pérez-Rius C, Castellanos A, Gaitán-Peñas H, Navarro A, Artuch R, Barrallo-Gimeno A, Estévez R
The Journal of physiology597(15)3969-3983(2019 Aug)

0# Diesel water-accommodated fraction induced lipid homeostasis alteration in zebrafish embryos.
Mu X, Liu J, Yang K, Huang Y, Li X, Yang W, Qi S, Tu W, Shen G, Li Y
Environmental pollution (Barking, Essex : 1987)242(Pt A)952-961(2018 Nov)

Evolution of complexity in the zebrafish synapse proteome.
Bayés À, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 03)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

The draft genome of the grass carp (Ctenopharyngodon idellus) provides insights into its evolution and vegetarian adaptation.
Wang Y, Lu Y, Zhang Y, Ning Z, Li Y, Zhao Q, Lu H, Huang R, Xia X, Feng Q, Liang X, Liu K, Zhang L, Lu T, Huang T, Fan D, Weng Q, Zhu C, Lu Y, Li W, Wen Z, Zhou C, Tian Q, Kang X, Shi M, Zhang W, Jang S, Du F, He S, Liao L, Li Y, Gui B, He H, Ning Z, Yang C, He L, Luo L, Yang R, Luo Q, Liu X, Li S, Huang W, Xiao L, Lin H, Han B, Zhu Z
Nature genetics47(6)625-31(2015 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following dhcr24 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dhcr24 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa31267	NM_001008645.1 Latest version!	Danio rerio 24-dehydrocholesterol reductase (dhcr24), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dhcr24 ( NM_001008645.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001008645.1 Danio rerio 24-dehydrocholesterol reductase (dhcr24), mRNA.

Price & Availability↑

CloneID	ODa31267
Clone ID Related Accession (Same CDS sequence)	NM_001008645.1
Accession Version	NM_001008645.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576771200000
Organism	Danio rerio(zebrafish)
Product	delta(24)-sterol reductase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC086711.1. ##Evidence-Data-START## Transcript exon combination :: BC086711.1, BC165211.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446 [ECO:0000348] ##Evidence-Data-END##

ATGGATCCGT TATTGTATTT GGGAGGGTTA GCTGTGTTGT TTTTGATATG GATCAAAGTG 
AAAGGCTTGG AGTACGTGAT TATACACCAG AGATGGATCT TCGTGTGCCT GTTTCTCCTG 
CCCTTGTCCG TCGTGTTTGA TGTGTACTAT CACCTGCGCG CCTGGATCAT CTTCAAGATG 
TGCTCCGCGC CCAAACAGCA CGACCAGCGG GTCAGAGACA TTCAGAGACA GGTGCGAGAA 
TGGAGGAAAG ATGGAGGGAA GAAATATATG TGCACGGGAC GTCCGGGATG GCTGACTGTG 
TCCCTCAGAG TGGGAAAATA CAAGAAAACT CACAAGAACA TCATGATTAA CATGATGGAC 
ATCCTGGAGG TTGACACAAA ACGAAAGGTA GTGCGTGTGG AGCCTTTAGC TAACATGGGT 
CAGGTGACTG CTCTGCTCAA CTCTATTGGC TGGACACTGC CGGTGCTGCC AGAACTCGAT 
GACCTCACTG TTGGCGGGCT GGTGATGGGA ACAGGCATTG AGTCTTCATC TCACATCTAC 
GGCCTGTTTC AGCACATCTG TGTAGCCTTT GAGCTGGTGT TGGCTGACGG CAGTCTGGTC 
CGCTGCACTG AGAAAGAAAA CTCTGACCTG TTTTATGCCG TTCCCTGGTC CTGCGGGACT 
CTGGGGTTTC TGGTTGCGGC AGAGATCCGG ATAATTCCAG CTCAGAAATG GGTGAAGCTG 
CACTATGAAC CTGTTCGCGG CTTGGATGCA ATTTGCAAAA AGTTTGCTGA GGAATCTGCC 
AATAAGGAGA ACCAGTTTGT TGAGGGACTT CAGTACTCTC GGGACGAGGC TGTGATTATG 
ACCGGCGTCA TGACGGATCA TGCAGAGCCT GACAAGACTA ACTGTATAGG TTATTATTAC 
AAGCCGTGGT TCTTTCGGCA TGTGGAGAGT TTCCTGAAGC AGAACCGCGT TGCAGTGGAG 
TACATTCCTC TCCGCCACTA TTACCACAGA CACACCCGCA GCATCTTCTG GGAGTTACAA 
GACATTATTC CATTCGGGAA TAACCCGTTG TTCCGGTATG TGTTTGGCTG GATGGTTCCT 
CCAAAGATCT CTCTGCTAAA GCTTACTCAG GGAGAGACCA TCCGCAAACT GTACGAGCAG 
CACCATGTGG TGCAGGACAT GCTGGTCCCC ATGAAGGACA TCAAGGCTGC CATCCAGCGT 
TTCCATGAGG ACATTCATGT GTATCCTCTG TGGCTGTGTC CATTCCTTTT GCCCAACCAG 
CCAGGAATGG TGCATCCTAA AGGAGATGAA GATGAACTGT ATGTTGATAT CGGAGCATAT 
GGAGAACCAA AGGTCAAACA CTTTGAGGCC ACATCATCCA CACGGCAGCT GGAGAAGTTT 
GTCAGAGACG TTCACGGATT CCAGATGTTG TATGCTGATG TATACATGGA ACGCAAGGAA 
TTCTGGGAGA TGTTTGACGG CACTTTGTAT CACAAACTCA GAGAGGAGCT CGGCTGTAAA 
GATGCATTCC CTGAAGTCTT TGACAAAATC TGCAAGTCTG CAAGACATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001008645.1
CDS	120..1670
Translation	MDPLLYLGGLAVLFLIWIKVKGLEYVIIHQRWIFVCLFLLPLSVVFDVYYHLRAWIIFKMCSAPKQHDQRVRDIQRQVREWRKDGGKKYMCTGRPGWLTVSLRVGKYKKTHKNIMINMMDILEVDTKRKVVRVEPLANMGQVTALLNSIGWTLPVLPELDDLTVGGLVMGTGIESSSHIYGLFQHICVAFELVLADGSLVRCTEKENSDLFYAVPWSCGTLGFLVAAEIRIIPAQKWVKLHYEPVRGLDAICKKFAEESANKENQFVEGLQYSRDEAVIMTGVMTDHAEPDKTNCIGYYYKPWFFRHVESFLKQNRVAVEYIPLRHYYHRHTRSIFWELQDIIPFGNNPLFRYVFGWMVPPKISLLKLTQGETIRKLYEQHHVVQDMLVPMKDIKAAIQRFHEDIHVYPLWLCPFLLPNQPGMVHPKGDEDELYVDIGAYGEPKVKHFEATSSTRQLEKFVRDVHGFQMLYADVYMERKEFWEMFDGTLYHKLREELGCKDAFPEVFDKICKSARH

Target ORF information:

RefSeq Version	NM_001008645.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio 24-dehydrocholesterol reductase (dhcr24), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001008645.1

ORF Insert Sequence:

ATGGATCCGT TATTGTATTT GGGAGGGTTA GCTGTGTTGT TTTTGATATG GATCAAAGTG 
AAAGGCTTGG AGTACGTGAT TATACACCAG AGATGGATCT TCGTGTGCCT GTTTCTCCTG 
CCCTTGTCCG TCGTGTTTGA TGTGTACTAT CACCTGCGCG CCTGGATCAT CTTCAAGATG 
TGCTCCGCGC CCAAACAGCA CGACCAGCGG GTCAGAGACA TTCAGAGACA GGTGCGAGAA 
TGGAGGAAAG ATGGAGGGAA GAAATATATG TGCACGGGAC GTCCGGGATG GCTGACTGTG 
TCCCTCAGAG TGGGAAAATA CAAGAAAACT CACAAGAACA TCATGATTAA CATGATGGAC 
ATCCTGGAGG TTGACACAAA ACGAAAGGTA GTGCGTGTGG AGCCTTTAGC TAACATGGGT 
CAGGTGACTG CTCTGCTCAA CTCTATTGGC TGGACACTGC CGGTGCTGCC AGAACTCGAT 
GACCTCACTG TTGGCGGGCT GGTGATGGGA ACAGGCATTG AGTCTTCATC TCACATCTAC 
GGCCTGTTTC AGCACATCTG TGTAGCCTTT GAGCTGGTGT TGGCTGACGG CAGTCTGGTC 
CGCTGCACTG AGAAAGAAAA CTCTGACCTG TTTTATGCCG TTCCCTGGTC CTGCGGGACT 
CTGGGGTTTC TGGTTGCGGC AGAGATCCGG ATAATTCCAG CTCAGAAATG GGTGAAGCTG 
CACTATGAAC CTGTTCGCGG CTTGGATGCA ATTTGCAAAA AGTTTGCTGA GGAATCTGCC 
AATAAGGAGA ACCAGTTTGT TGAGGGACTT CAGTACTCTC GGGACGAGGC TGTGATTATG 
ACCGGCGTCA TGACGGATCA TGCAGAGCCT GACAAGACTA ACTGTATAGG TTATTATTAC 
AAGCCGTGGT TCTTTCGGCA TGTGGAGAGT TTCCTGAAGC AGAACCGCGT TGCAGTGGAG 
TACATTCCTC TCCGCCACTA TTACCACAGA CACACCCGCA GCATCTTCTG GGAGTTACAA 
GACATTATTC CATTCGGGAA TAACCCGTTG TTCCGGTATG TGTTTGGCTG GATGGTTCCT 
CCAAAGATCT CTCTGCTAAA GCTTACTCAG GGAGAGACCA TCCGCAAACT GTACGAGCAG 
CACCATGTGG TGCAGGACAT GCTGGTCCCC ATGAAGGACA TCAAGGCTGC CATCCAGCGT 
TTCCATGAGG ACATTCATGT GTATCCTCTG TGGCTGTGTC CATTCCTTTT GCCCAACCAG 
CCAGGAATGG TGCATCCTAA AGGAGATGAA GATGAACTGT ATGTTGATAT CGGAGCATAT 
GGAGAACCAA AGGTCAAACA CTTTGAGGCC ACATCATCCA CACGGCAGCT GGAGAAGTTT 
GTCAGAGACG TTCACGGATT CCAGATGTTG TATGCTGATG TATACATGGA ACGCAAGGAA 
TTCTGGGAGA TGTTTGACGG CACTTTGTAT CACAAACTCA GAGAGGAGCT CGGCTGTAAA 
GATGCATTCC CTGAAGTCTT TGACAAAATC TGCAAGTCTG CAAGACATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Role of zebrafish ClC-K/barttin channels in apical kidney chloride reabsorption.
The Journal of physiology597(15)3969-3983(2019 Aug)
Pérez-Rius C,Castellanos A,Gaitán-Peñas H,Navarro A,Artuch R,Barrallo-Gimeno A,Estévez R

0# Diesel water-accommodated fraction induced lipid homeostasis alteration in zebrafish embryos.
Environmental pollution (Barking, Essex : 1987)242(Pt A)952-961(2018 Nov)
Mu X,Liu J,Yang K,Huang Y,Li X,Yang W,Qi S,Tu W,Shen G,Li Y

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 03)
Bayés À,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

The draft genome of the grass carp (Ctenopharyngodon idellus) provides insights into its evolution and vegetarian adaptation.
Nature genetics47(6)625-31(2015 Jun)
Wang Y,Lu Y,Zhang Y,Ning Z,Li Y,Zhao Q,Lu H,Huang R,Xia X,Feng Q,Liang X,Liu K,Zhang L,Lu T,Huang T,Fan D,Weng Q,Zhu C,Lu Y,Li W,Wen Z,Zhou C,Tian Q,Kang X,Shi M,Zhang W,Jang S,Du F,He S,Liao L,Li Y,Gui B,He H,Ning Z,Yang C,He L,Luo L,Yang R,Luo Q,Liu X,Li S,Huang W,Xiao L,Lin H,Han B,Zhu Z

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dhcr24 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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