pcf11 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	pcf11
Entrez Gene ID	492341
Full Name	PCF11 cleavage and polyadenylation factor subunit
Synonyms	im:6912257,im:7138745,wu:fc47a05,zgc:175013,zgc:92226
General protein information	Preferred Names PCF11 cleavage and polyadenylation factor subunit Names pre-mRNA cleavage complex 2 protein Pcf11 cleavage and polyadenylation factor subunit, homolog
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001007307.1	NP_001007308.1	pre-mRNA cleavage complex 2 protein Pcf11

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PCF11	XP_508673.4
Macaca mulatta (Rhesus monkey)	PCF11	XP_002808130.1
Gallus gallus (chicken)	PCF11	XP_417214.3
Danio rerio (zebrafish)	pcf11	NP_001007308.1
Homo sapiens (human)	PCF11	NP_056969.2
Bos taurus (cattle)	LOC510604	XP_002699113.2
Canis lupus familiaris (dog)	PCF11	XP_533992.4
Mus musculus (house mouse)	Pcf11	NP_083354.3
Rattus norvegicus (Norway rat)	Pcf11	XP_341884.4

Gene Ontology
Bibliography

Related articles in PubMed

Selective Roles of Vertebrate PCF11 in Premature and Full-Length Transcript Termination.
Kamieniarz-Gdula K, Gdula MR, Panser K, Nojima T, Monks J, Wiśniewski JR, Riepsaame J, Brockdorff N, Pauli A, Proudfoot NJ
Molecular cell74(1)158-172.e9(2019 04)

Nanog, Pou5f1 and SoxB1 activate zygotic gene expression during the maternal-to-zygotic transition.
Lee MT, Bonneau AR, Takacs CM, Bazzini AA, DiVito KR, Fleming ES, Giraldez AJ
Nature503(7476)360-4(2013 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following pcf11 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pcf11 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03388	NM_001007307.1 Latest version!	Danio rerio PCF11 cleavage and polyadenylation factor subunit (pcf11), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pcf11 ( NM_001007307.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001007308.1 Danio rerio PCF11 cleavage and polyadenylation factor subunit (pcf11), mRNA.

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CloneID	ODa03388
Clone ID Related Accession (Same CDS sequence)	NM_001007307.1
Accession Version	NM_001007307.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1563724800000
Organism	Danio rerio(zebrafish)
Product	pre-mRNA cleavage complex 2 protein Pcf11
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC085652.1. ##Evidence-Data-START## Transcript exon combination :: BC085652.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTCGGACG ACGGTGCGAG GGAGGACGCC TGCCGCGAGT ATCAGTCCTC GCTGGAAGAT 
CTGACTTTCA ACAGCAAACC GCACATCAAC ATGCTCACCA TTCTAGCCGA GGAAAACATC 
CAGTTCACCA AGGATATCGT CGCAATAATT GAAGCACAAA TTGCCAAGGC ACCTCCTGTT 
GAGAAGCTTC CAGTTTTGTA CTTAGTGGAT TCCATAGTGA AGAATGTTGG AGGGGCTTAC 
CTTGAAGTGT TTGCTAAGAA CCTAGTCAAT TCCTTTATTT GTGTATTTGA AAAGGTTGAT 
GAGGGCACTA GAAAAAGTCT CTTCAAATTG CGTTCTACAT GGGATGAAAT TTTCCCCTTG 
AAGAAACTAT ATGCACTTGA TGTGCGTGTG AACTCTGTAG ATCCTGCATG GCCCATTAAA 
CCATTGCCAC CAAATGTGAA TGCCAGCATT CATGTCAACC CCAAATTTTT AAAACCGACT 
GAAGAAACGC CCCCGAAGCC TACACCACCA CCTCCAACAC CAAGCTTGAC TCAAGAGCAG 
CTGATAAGGC AACAGTTGCT TGCAAAGCAA AAGCAGTTGT TAGAACTTCA GCAAAAGAAG 
ATAGAGCTTG AACTTGAACA AACAAAAGCT CAGTTGGCTG CCAGTCCATC TCTGGCTTTA 
AATCATCATG CCAGTGCTCC AGTCCGTTTC AATGCCTCAC CAAAGGTTAA TCCAACGGTT 
CCTGTTCCGC AGACCAAATC TTGGCCTCCT GCCCCATCAG AAAAATTGTC AACCAGAGAT 
CCTAGATTAA ACAGATCTGG TACAACAGCT GTAAATCCAA AAGAACAAGT GACGCATAAA 
AAGGAGAGTC CAGCTGCACC AAGCTTTCAA AATGCTCCAG AAAAAAGAGG ACAAGCCTCA 
TCTGAAAGAC CGGCTAAGTT GGATAAGTTG AGAATTCCAA AAAAGGATAA CTCTGCTGTT 
GAAGAAAAGT CTAAATCCAA GTCTATGTTG CCATCTGGAA AAGTGATGCC TGTAAGACCT 
AGAGGATTGG AGTCTGAACA ATCTAAGTCT GCTGAAGTCA ACAAAAAGGA CCCAAGGCTA 
CATAAGCAGA TGCATGAAAG GATGGATTCA AAAGATGAAG ATGTTCGAGA GAAGAAAAGA 
AGCTCAGAGA AAAAAGATAG GGATGAAGGT TCCAAAAATT TGGATCATCA GAAGTTAAGC 
AGCAACAGAG GCAAACTTAT AAATGGCTCT GTGAACAAGC ATGAAAAGCT AGAGACCTTT 
CTGAAACAGG AGATCAAAGT GAACAAAGCA AATGTTAGAA AAAGGTCAAG ATCGCGTTCT 
CCACCCGTAC ATTCTCCTAA AAGAAAAGAG AGGCGATCGT CTCCAAAGAG AAAAACCAGG 
AGCATTAGTC CACCTCCCAA GTCTGGAAAG TCTAGACTCA GTAAGCATCC ACATGATGAT 
GCTTTCCCAC AACCAAGTGC AAGAGATGAA CGTACGAAGA AAAGTGTTCC TGATTCGAGA 
CGACCAAAAA GGCCTCTTGA GGATAGACCT GCTGAAAAGA AAGATGGATC ACTGCAAAGA 
ATTTCTGCTG CAGAGCATAA AGATCTAAAA GATGGCAAGA GGTGGAGGAG TGGATGGGAA 
GAAAACAAAC ATCCTAAACA CTCAGATACA GATCTGTCAC ATGGGCGAAT GGGGATCCAG 
AAACACAAAA CTTGGAACAC TAATCAAAGA CCACCCACAC CTCGGACACC TAAGCAACAT 
CGCTTAAGTG TTGATAGCAA CATACAAATA CCAGAAGCAC TGCATTCTGC TAGTAAACGG 
GATCTTTTAA GAAAGGCTAG CAAAAGGCGT GCTGATGGCG AAATATCCAA TGATGAATTT 
CTCAGCGTAG CTCATCAAAT CAACCAACTT TTTCAATATC AAGAAGAAAG GCAACGATCA 
GATTCTTGGG ATGAATCATG TGATGAGGGA GTGTATCCAT CAAGAAAGAA ACAAGAAACC 
ATATCTGATG CTTACCTTGA GCATAAATTA AAGTTGAGAA GAACTCAGCT CCACCGTCCA 
GTGCAAAGAG GGGGACATTT ACCTTTGCAT GAAATGTATC ACTATCCGCC TCATCATGAA 
GTATCTGAAC AATATAGTGA AAGCTTGGAT GTGCATAAAA TGTCAGGTGA TCCCATTAAG 
CCACTCTCTG ACCATGAAGA TCACAGAAGA ATTGACAGGC CTCCATCCTG CACAGGGTCT 
ACTTTCAGAA ATTCGCCAAG CCCAGTTGGC TTAGATGGTA TCTGTGGAAA ATCCACAGTA 
CTTCCATTTG AACGGCCATC CTCACCATCT GAAATGGATC AACAACCTGA GGGAGACATC 
AGCCCACGTT TTGAAAGTCC CAACAGTGTG CACTCTGGCA CAGGCCCAGA TGATGGTCCA 
ATTAGCGTTG AGGGTCTTCC CAGGCACGAT CATTTCTTGG AACAAGGACG AAGTGGGCGA 
ATCCATGGTG AATCACCTGG AAATACACCA AGTCACTCAG AAGGACCTAC AACGCAGGTC 
AATGCAGCAC GGCATGATGG ACCAATTCAT CAACGTTTTG ATAGGTACAA AAATCCTCAG 
GCTCCCTTTG ATGGACCATC TAGCCACATG AGAGAGCCAA GATTGGATGG ACCTCCAAGA 
CCATTTGTTC CACCTTCACG ATATGAAAGC AATACAGGGG GATTTGATGG GTCAGGTGGA 
CCTGTAAGGT TTTCCGGACA CCGCTTTGAT ACCCCTCACC ACTTTGAACA ATTTCCCAAA 
GCACCTGAAA GACCTGTGAG ATTTAACAAC CCACAAGTTT CACATAGGCC AATGCGCTTC 
GTAGAGCCTC ATAATGTTAG ATTTGACTCC CCAACACCGG TTCATTATGA TCACTCAATG 
CCCCAAAACA GATTTGTTAA TCCACCAAGG TTCGATAACC CTCAAATGCA ACAGGGCCCA 
CCAGGATATG AGGAACCACA TTTTCCAGCT AGGATAATTA ATTATGATGA ACAGCAGGGT 
CCAGTTAGAT TTGATAACCC AACATGTGGG ATTCGTTTTG AGAACCCTGT GCAGCCTGAA 
CCCTTAAGGT TCGATGCACC ACCTGTCATG CCAAGATATG ACCCACAGGG CCCTCCAAGA 
TACTGCGGTC CAAATATTCC AAACCAGCTA AGACCACAGG AACCAACAAT GTATGATCAA 
ACTCAAGGTC AAGGTCCAAT GATAAACCCT ACTGTACCCC CACCCAATTT CAACATGCCA 
CCCATAAACT CATTTGGTGG TCCAGCCCAG CAGTTTTCCA TGCAGCAAAA TGTCTCGCAA 
ACCTCCAACT TCAGTGTGCC AGTTCCTACA TCATCAGATT TTCAGGGTTC ATTTAGACCT 
CCATTCCCTG GTCCGGGTGT CGGAGGTGTT CCTCAGCCTA TGATGGGTGC TCAAAACTTC 
ATGCCCCAAA ATCCAATGCC TTTTCAGCCA GTCTCCCAAT TTCCACAGCC TGAGCCAGAG 
CCATTGAGAC AAATAGATGT GAATGATCTG ATGTCTAAGC TTATATCCAC TGGAATTATT 
AAACCTGCAC CTACAGACTC AACAGCTGAC TCCCCATCAG CTAGTCAGAC TATATCTGTG 
CCTGAAGAAG AGGAGGAGGA GGAGGAGCAA GAGCAAGAAG AAGATGACAG TGTCCCAGAT 
TTGACTGGCT TTATTTTGGA TGACATGAAA CAGAGACACG AAAGCATTAT CACTAAACTC 
TATACTGGAA TCCAGTGCTA TTCCTGTGGC ATGCGTTTTA CTGCATCTCA GACAGACGTG 
TATGCAGATC ATTTGGACTG GCACTACAGA CAGAACCGCT CAGAGAAAGA CATCAGTAAG 
AAAGTCACGC ATCGCCGCTG GTACTACAGT CTGACGGATT GGATTGAATT TGAGGAAATC 
GCTGACCTTG AAGAGAGGGC AAAAAGTCAG TTCTTTGAAA AAGTTCATGA AGAGGTTGTA 
CAGAAAACTC AGGAGGCAGC CAAAGAAAGG GAGTTCCAGA GCGTGAAGGC TGCAGCGGAT 
GTCGTTCATG AGTTGTGCGA GATTTGCCAG GAGCAGTTTG AAATGTACTG GGAGGAGGAA 
GAGGAAGAGT GGCACCTGAA GAATGCCATC AGAGTTGATG AAAAGACCTA TCATCCCTCA 
TGTTATGAAG ACTACAAAAA TACATCCTCC TTTATGGACA CCACACCCTC GCCCAATAAA 
ATGCTTACAG AAAATCCCTT AACAGCTTTT TTGAAACAAG AGCAGGATGA CGAGACGTCG 
TGTTCCAGTA TTAAAGAAGA GCCCGCTGAT GATGATATGG AGGCTCCAAT GGTAAAAGAG 
GAGGTTCAGG TTAAGTTAGA AGGAGAATCA CAAACCAGTG CAATTGTTTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001007308.1
CDS	182..4555
Translation	MSDDGAREDACREYQSSLEDLTFNSKPHINMLTILAEENIQFTKDIVAIIEAQIAKAPPVEKLPVLYLVDSIVKNVGGAYLEVFAKNLVNSFICVFEKVDEGTRKSLFKLRSTWDEIFPLKKLYALDVRVNSVDPAWPIKPLPPNVNASIHVNPKFLKPTEETPPKPTPPPPTPSLTQEQLIRQQLLAKQKQLLELQQKKIELELEQTKAQLAASPSLALNHHASAPVRFNASPKVNPTVPVPQTKSWPPAPSEKLSTRDPRLNRSGTTAVNPKEQVTHKKESPAAPSFQNAPEKRGQASSERPAKLDKLRIPKKDNSAVEEKSKSKSMLPSGKVMPVRPRGLESEQSKSAEVNKKDPRLHKQMHERMDSKDEDVREKKRSSEKKDRDEGSKNLDHQKLSSNRGKLINGSVNKHEKLETFLKQEIKVNKANVRKRSRSRSPPVHSPKRKERRSSPKRKTRSISPPPKSGKSRLSKHPHDDAFPQPSARDERTKKSVPDSRRPKRPLEDRPAEKKDGSLQRISAAEHKDLKDGKRWRSGWEENKHPKHSDTDLSHGRMGIQKHKTWNTNQRPPTPRTPKQHRLSVDSNIQIPEALHSASKRDLLRKASKRRADGEISNDEFLSVAHQINQLFQYQEERQRSDSWDESCDEGVYPSRKKQETISDAYLEHKLKLRRTQLHRPVQRGGHLPLHEMYHYPPHHEVSEQYSESLDVHKMSGDPIKPLSDHEDHRRIDRPPSCTGSTFRNSPSPVGLDGICGKSTVLPFERPSSPSEMDQQPEGDISPRFESPNSVHSGTGPDDGPISVEGLPRHDHFLEQGRSGRIHGESPGNTPSHSEGPTTQVNAARHDGPIHQRFDRYKNPQAPFDGPSSHMREPRLDGPPRPFVPPSRYESNTGGFDGSGGPVRFSGHRFDTPHHFEQFPKAPERPVRFNNPQVSHRPMRFVEPHNVRFDSPTPVHYDHSMPQNRFVNPPRFDNPQMQQGPPGYEEPHFPARIINYDEQQGPVRFDNPTCGIRFENPVQPEPLRFDAPPVMPRYDPQGPPRYCGPNIPNQLRPQEPTMYDQTQGQGPMINPTVPPPNFNMPPINSFGGPAQQFSMQQNVSQTSNFSVPVPTSSDFQGSFRPPFPGPGVGGVPQPMMGAQNFMPQNPMPFQPVSQFPQPEPEPLRQIDVNDLMSKLISTGIIKPAPTDSTADSPSASQTISVPEEEEEEEEQEQEEDDSVPDLTGFILDDMKQRHESIITKLYTGIQCYSCGMRFTASQTDVYADHLDWHYRQNRSEKDISKKVTHRRWYYSLTDWIEFEEIADLEERAKSQFFEKVHEEVVQKTQEAAKEREFQSVKAAADVVHELCEICQEQFEMYWEEEEEEWHLKNAIRVDEKTYHPSCYEDYKNTSSFMDTTPSPNKMLTENPLTAFLKQEQDDETSCSSIKEEPADDDMEAPMVKEEVQVKLEGESQTSAIVF

Target ORF information:

RefSeq Version	NM_001007307.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio PCF11 cleavage and polyadenylation factor subunit (pcf11), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001007307.1

ORF Insert Sequence:

ATGTCGGACG ACGGTGCGAG GGAGGACGCC TGCCGCGAGT ATCAGTCCTC GCTGGAAGAT 
CTGACTTTCA ACAGCAAACC GCACATCAAC ATGCTCACCA TTCTAGCCGA GGAAAACATC 
CAGTTCACCA AGGATATCGT CGCAATAATT GAAGCACAAA TTGCCAAGGC ACCTCCTGTT 
GAGAAGCTTC CAGTTTTGTA CTTAGTGGAT TCCATAGTGA AGAATGTTGG AGGGGCTTAC 
CTTGAAGTGT TTGCTAAGAA CCTAGTCAAT TCCTTTATTT GTGTATTTGA AAAGGTTGAT 
GAGGGCACTA GAAAAAGTCT CTTCAAATTG CGTTCTACAT GGGATGAAAT TTTCCCCTTG 
AAGAAACTAT ATGCACTTGA TGTGCGTGTG AACTCTGTAG ATCCTGCATG GCCCATTAAA 
CCATTGCCAC CAAATGTGAA TGCCAGCATT CATGTCAACC CCAAATTTTT AAAACCGACT 
GAAGAAACGC CCCCGAAGCC TACACCACCA CCTCCAACAC CAAGCTTGAC TCAAGAGCAG 
CTGATAAGGC AACAGTTGCT TGCAAAGCAA AAGCAGTTGT TAGAACTTCA GCAAAAGAAG 
ATAGAGCTTG AACTTGAACA AACAAAAGCT CAGTTGGCTG CCAGTCCATC TCTGGCTTTA 
AATCATCATG CCAGTGCTCC AGTCCGTTTC AATGCCTCAC CAAAGGTTAA TCCAACGGTT 
CCTGTTCCGC AGACCAAATC TTGGCCTCCT GCCCCATCAG AAAAATTGTC AACCAGAGAT 
CCTAGATTAA ACAGATCTGG TACAACAGCT GTAAATCCAA AAGAACAAGT GACGCATAAA 
AAGGAGAGTC CAGCTGCACC AAGCTTTCAA AATGCTCCAG AAAAAAGAGG ACAAGCCTCA 
TCTGAAAGAC CGGCTAAGTT GGATAAGTTG AGAATTCCAA AAAAGGATAA CTCTGCTGTT 
GAAGAAAAGT CTAAATCCAA GTCTATGTTG CCATCTGGAA AAGTGATGCC TGTAAGACCT 
AGAGGATTGG AGTCTGAACA ATCTAAGTCT GCTGAAGTCA ACAAAAAGGA CCCAAGGCTA 
CATAAGCAGA TGCATGAAAG GATGGATTCA AAAGATGAAG ATGTTCGAGA GAAGAAAAGA 
AGCTCAGAGA AAAAAGATAG GGATGAAGGT TCCAAAAATT TGGATCATCA GAAGTTAAGC 
AGCAACAGAG GCAAACTTAT AAATGGCTCT GTGAACAAGC ATGAAAAGCT AGAGACCTTT 
CTGAAACAGG AGATCAAAGT GAACAAAGCA AATGTTAGAA AAAGGTCAAG ATCGCGTTCT 
CCACCCGTAC ATTCTCCTAA AAGAAAAGAG AGGCGATCGT CTCCAAAGAG AAAAACCAGG 
AGCATTAGTC CACCTCCCAA GTCTGGAAAG TCTAGACTCA GTAAGCATCC ACATGATGAT 
GCTTTCCCAC AACCAAGTGC AAGAGATGAA CGTACGAAGA AAAGTGTTCC TGATTCGAGA 
CGACCAAAAA GGCCTCTTGA GGATAGACCT GCTGAAAAGA AAGATGGATC ACTGCAAAGA 
ATTTCTGCTG CAGAGCATAA AGATCTAAAA GATGGCAAGA GGTGGAGGAG TGGATGGGAA 
GAAAACAAAC ATCCTAAACA CTCAGATACA GATCTGTCAC ATGGGCGAAT GGGGATCCAG 
AAACACAAAA CTTGGAACAC TAATCAAAGA CCACCCACAC CTCGGACACC TAAGCAACAT 
CGCTTAAGTG TTGATAGCAA CATACAAATA CCAGAAGCAC TGCATTCTGC TAGTAAACGG 
GATCTTTTAA GAAAGGCTAG CAAAAGGCGT GCTGATGGCG AAATATCCAA TGATGAATTT 
CTCAGCGTAG CTCATCAAAT CAACCAACTT TTTCAATATC AAGAAGAAAG GCAACGATCA 
GATTCTTGGG ATGAATCATG TGATGAGGGA GTGTATCCAT CAAGAAAGAA ACAAGAAACC 
ATATCTGATG CTTACCTTGA GCATAAATTA AAGTTGAGAA GAACTCAGCT CCACCGTCCA 
GTGCAAAGAG GGGGACATTT ACCTTTGCAT GAAATGTATC ACTATCCGCC TCATCATGAA 
GTATCTGAAC AATATAGTGA AAGCTTGGAT GTGCATAAAA TGTCAGGTGA TCCCATTAAG 
CCACTCTCTG ACCATGAAGA TCACAGAAGA ATTGACAGGC CTCCATCCTG CACAGGGTCT 
ACTTTCAGAA ATTCGCCAAG CCCAGTTGGC TTAGATGGTA TCTGTGGAAA ATCCACAGTA 
CTTCCATTTG AACGGCCATC CTCACCATCT GAAATGGATC AACAACCTGA GGGAGACATC 
AGCCCACGTT TTGAAAGTCC CAACAGTGTG CACTCTGGCA CAGGCCCAGA TGATGGTCCA 
ATTAGCGTTG AGGGTCTTCC CAGGCACGAT CATTTCTTGG AACAAGGACG AAGTGGGCGA 
ATCCATGGTG AATCACCTGG AAATACACCA AGTCACTCAG AAGGACCTAC AACGCAGGTC 
AATGCAGCAC GGCATGATGG ACCAATTCAT CAACGTTTTG ATAGGTACAA AAATCCTCAG 
GCTCCCTTTG ATGGACCATC TAGCCACATG AGAGAGCCAA GATTGGATGG ACCTCCAAGA 
CCATTTGTTC CACCTTCACG ATATGAAAGC AATACAGGGG GATTTGATGG GTCAGGTGGA 
CCTGTAAGGT TTTCCGGACA CCGCTTTGAT ACCCCTCACC ACTTTGAACA ATTTCCCAAA 
GCACCTGAAA GACCTGTGAG ATTTAACAAC CCACAAGTTT CACATAGGCC AATGCGCTTC 
GTAGAGCCTC ATAATGTTAG ATTTGACTCC CCAACACCGG TTCATTATGA TCACTCAATG 
CCCCAAAACA GATTTGTTAA TCCACCAAGG TTCGATAACC CTCAAATGCA ACAGGGCCCA 
CCAGGATATG AGGAACCACA TTTTCCAGCT AGGATAATTA ATTATGATGA ACAGCAGGGT 
CCAGTTAGAT TTGATAACCC AACATGTGGG ATTCGTTTTG AGAACCCTGT GCAGCCTGAA 
CCCTTAAGGT TCGATGCACC ACCTGTCATG CCAAGATATG ACCCACAGGG CCCTCCAAGA 
TACTGCGGTC CAAATATTCC AAACCAGCTA AGACCACAGG AACCAACAAT GTATGATCAA 
ACTCAAGGTC AAGGTCCAAT GATAAACCCT ACTGTACCCC CACCCAATTT CAACATGCCA 
CCCATAAACT CATTTGGTGG TCCAGCCCAG CAGTTTTCCA TGCAGCAAAA TGTCTCGCAA 
ACCTCCAACT TCAGTGTGCC AGTTCCTACA TCATCAGATT TTCAGGGTTC ATTTAGACCT 
CCATTCCCTG GTCCGGGTGT CGGAGGTGTT CCTCAGCCTA TGATGGGTGC TCAAAACTTC 
ATGCCCCAAA ATCCAATGCC TTTTCAGCCA GTCTCCCAAT TTCCACAGCC TGAGCCAGAG 
CCATTGAGAC AAATAGATGT GAATGATCTG ATGTCTAAGC TTATATCCAC TGGAATTATT 
AAACCTGCAC CTACAGACTC AACAGCTGAC TCCCCATCAG CTAGTCAGAC TATATCTGTG 
CCTGAAGAAG AGGAGGAGGA GGAGGAGCAA GAGCAAGAAG AAGATGACAG TGTCCCAGAT 
TTGACTGGCT TTATTTTGGA TGACATGAAA CAGAGACACG AAAGCATTAT CACTAAACTC 
TATACTGGAA TCCAGTGCTA TTCCTGTGGC ATGCGTTTTA CTGCATCTCA GACAGACGTG 
TATGCAGATC ATTTGGACTG GCACTACAGA CAGAACCGCT CAGAGAAAGA CATCAGTAAG 
AAAGTCACGC ATCGCCGCTG GTACTACAGT CTGACGGATT GGATTGAATT TGAGGAAATC 
GCTGACCTTG AAGAGAGGGC AAAAAGTCAG TTCTTTGAAA AAGTTCATGA AGAGGTTGTA 
CAGAAAACTC AGGAGGCAGC CAAAGAAAGG GAGTTCCAGA GCGTGAAGGC TGCAGCGGAT 
GTCGTTCATG AGTTGTGCGA GATTTGCCAG GAGCAGTTTG AAATGTACTG GGAGGAGGAA 
GAGGAAGAGT GGCACCTGAA GAATGCCATC AGAGTTGATG AAAAGACCTA TCATCCCTCA 
TGTTATGAAG ACTACAAAAA TACATCCTCC TTTATGGACA CCACACCCTC GCCCAATAAA 
ATGCTTACAG AAAATCCCTT AACAGCTTTT TTGAAACAAG AGCAGGATGA CGAGACGTCG 
TGTTCCAGTA TTAAAGAAGA GCCCGCTGAT GATGATATGG AGGCTCCAAT GGTAAAAGAG 
GAGGTTCAGG TTAAGTTAGA AGGAGAATCA CAAACCAGTG CAATTGTTTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Selective Roles of Vertebrate PCF11 in Premature and Full-Length Transcript Termination.
Molecular cell74(1)158-172.e9(2019 04)
Kamieniarz-Gdula K,Gdula MR,Panser K,Nojima T,Monks J,Wiśniewski JR,Riepsaame J,Brockdorff N,Pauli A,Proudfoot NJ

Nanog, Pou5f1 and SoxB1 activate zygotic gene expression during the maternal-to-zygotic transition.
Nature503(7476)360-4(2013 Nov)
Lee MT,Bonneau AR,Takacs CM,Bazzini AA,DiVito KR,Fleming ES,Giraldez AJ

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pcf11 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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