sc5d cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	sc5d
Entrez Gene ID	447891
Full Name	sterol-C5-desaturase
Synonyms	sc5dl,zgc:101732
General protein information	Preferred Names sterol-C5-desaturase Names lathosterol oxidase sterol-C5-desaturase (ERG3 delta-5-desaturase homolog, S. cerevisiae)-like
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001004630.1	NP_001004630.1	lathosterol oxidase

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SC5DL	XP_508825.1
Mus musculus (house mouse)	Sc5d	NP_766357.1
Eremothecium gossypii	AGOS_AFR151C	NP_985698.2
Schizosaccharomyces pombe (fission yeast)	erg32	NP_001018791.2
Gallus gallus (chicken)	SC5DL	XP_003642637.2
Canis lupus familiaris (dog)	SC5D	XP_853004.1
Rattus norvegicus (Norway rat)	Sc5d	NP_446094.1
Kluyveromyces lactis	KLLA0F08855g	XP_455482.1
Arabidopsis thaliana (thale cress)	AT3G02590	NP_186908.1
Oryza sativa (rice)	Os01g0134500	NP_001041944.1
Xenopus tropicalis (tropical clawed frog)	LOC100490845	XP_002941684.1
Schizosaccharomyces pombe (fission yeast)	erg31	NP_593135.1
Homo sapiens (human)	SC5D	NP_001020127.1
Danio rerio (zebrafish)	sc5d	NP_001004630.1
Saccharomyces cerevisiae (baker's yeast)	ERG3	NP_013157.1
Magnaporthe oryzae (rice blast fungus)	MGG_08832	XP_003713851.1
Macaca mulatta (Rhesus monkey)	SC5DL	XP_001107388.1
Arabidopsis thaliana (thale cress)	STE1	NP_186907.1
Neurospora crassa	NCU06207	XP_962923.1

Gene Ontology
Bibliography

Related articles in PubMed

Lxr regulates lipid metabolic and visual perception pathways during zebrafish development.
Pinto CL, Kalasekar SM, McCollum CW, Riu A, Jonsson P, Lopez J, Swindell EC, Bouhlatouf A, Balaguer P, Bondesson M, Gustafsson JÅ
Molecular and cellular endocrinology41929-43(2016 Jan)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

The draft genome of the grass carp (Ctenopharyngodon idellus) provides insights into its evolution and vegetarian adaptation.
Wang Y, Lu Y, Zhang Y, Ning Z, Li Y, Zhao Q, Lu H, Huang R, Xia X, Feng Q, Liang X, Liu K, Zhang L, Lu T, Huang T, Fan D, Weng Q, Zhu C, Lu Y, Li W, Wen Z, Zhou C, Tian Q, Kang X, Shi M, Zhang W, Jang S, Du F, He S, Liao L, Li Y, Gui B, He H, Ning Z, Yang C, He L, Luo L, Yang R, Luo Q, Liu X, Li S, Huang W, Xiao L, Lin H, Han B, Zhu Z
Nature genetics47(6)625-31(2015 Jun)

Interspecies protein-protein interaction network construction for characterization of host-pathogen interactions: a Candida albicans-zebrafish interaction study.
Wang YC, Lin C, Chuang MT, Hsieh WP, Lan CY, Chuang YJ, Chen BS
BMC systems biology779(2013 Aug)

The zebrafish gene map defines ancestral vertebrate chromosomes.
Woods IG, Wilson C, Friedlander B, Chang P, Reyes DK, Nix R, Kelly PD, Chu F, Postlethwait JH, Talbot WS
Genome research15(9)1307-14(2005 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following sc5d gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sc5d cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa36048	NM_001004630.1 Latest version!	Danio rerio sterol-C5-desaturase (sc5d), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sc5d ( NM_001004630.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001004630.1 Danio rerio sterol-C5-desaturase (sc5d), mRNA.

Price & Availability↑

CloneID	ODa36048
Clone ID Related Accession (Same CDS sequence)	NM_001004630.1
Accession Version	NM_001004630.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1542902400000
Organism	Danio rerio(zebrafish)
Product	lathosterol oxidase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC081395.1. ##Evidence-Data-START## Transcript exon combination :: BC081395.1, CN016284.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGACCTTG TGCTGAAAGT TGCAGATCAT TACTTCTTTA CTCCATATGT ATATCCATCA 
TCATGGCCAG AAGACAATCC TCTGCGCCAA ATCATCGGTC TGATGGTGGT CACCAACCTG 
GGTGCTGCAA TTTTATACCT GGGCCTGGGT GCTTTGAGCT ACTTTTTTGT TTTCGACCAC 
AAATTAAAAG ACCACCCTCA ATTTTTGGAG AATCAGGTGC AACGTGAAAT AAAGTATGCT 
TTGTGGTCTC TGCCCTGGAT CAGCATACCC ACAGTAGCAC TGTTCTTCGC AGAGGTCAGA 
GGATACAGCA AACTCTATGA CAGAGTCGAT GATTCTCCTC TTGGTTGGTC AGGGCTGATT 
TTCAGCATGG TCTCTTTCCT GTTTTTCACT GACATGTGCA TTTACTGGAT TCACAGGTTC 
CTTCACCACA AGTTGATATA TAAGTATTTC CACAAACCAC ATCACGTTTG GAAGATTCCC 
ACACCATTCG CCAGCCATGC TTTCCACCCT GTGGACGGAT TTCTTCAGGG CCTGCCGTAC 
CACATCTACC CCTTCTTCTT TCCTCTGCAC AAGGTTCTGT ACTTAATCCT GTACGTGTTT 
GTCAACATAT GGACCATCTC CATCCACGAC GGGGACTATC GTGTACCCAA CATGGTGGAG 
GAGATCATCA ATGGCTCCGC TCACCACACT GACCACCACC TCTTCTTTGA CTACAACTAC 
GGCCAGTACT TCACCCTGTG GGACCGTATT GGAGGATCCT ACCGCTACCC TTCGGCCTTG 
ATGGGCAAAG GGCCACACGA CCAAATTAAA AAACTGATGG CAGAAGGGAA ACTGATCTCA 
AACAGCTCAA AGGGCCACAC TAACAATAAT CAAAAAAATG GTATTCACGT CAAGAAAGAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001004630.1
CDS	53..955
Translation	MDLVLKVADHYFFTPYVYPSSWPEDNPLRQIIGLMVVTNLGAAILYLGLGALSYFFVFDHKLKDHPQFLENQVQREIKYALWSLPWISIPTVALFFAEVRGYSKLYDRVDDSPLGWSGLIFSMVSFLFFTDMCIYWIHRFLHHKLIYKYFHKPHHVWKIPTPFASHAFHPVDGFLQGLPYHIYPFFFPLHKVLYLILYVFVNIWTISIHDGDYRVPNMVEEIINGSAHHTDHHLFFDYNYGQYFTLWDRIGGSYRYPSALMGKGPHDQIKKLMAEGKLISNSSKGHTNNNQKNGIHVKKE

Target ORF information:

RefSeq Version	NM_001004630.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sterol-C5-desaturase (sc5d), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001004630.1

ORF Insert Sequence:

ATGGACCTTG TGCTGAAAGT TGCAGATCAT TACTTCTTTA CTCCATATGT ATATCCATCA 
TCATGGCCAG AAGACAATCC TCTGCGCCAA ATCATCGGTC TGATGGTGGT CACCAACCTG 
GGTGCTGCAA TTTTATACCT GGGCCTGGGT GCTTTGAGCT ACTTTTTTGT TTTCGACCAC 
AAATTAAAAG ACCACCCTCA ATTTTTGGAG AATCAGGTGC AACGTGAAAT AAAGTATGCT 
TTGTGGTCTC TGCCCTGGAT CAGCATACCC ACAGTAGCAC TGTTCTTCGC AGAGGTCAGA 
GGATACAGCA AACTCTATGA CAGAGTCGAT GATTCTCCTC TTGGTTGGTC AGGGCTGATT 
TTCAGCATGG TCTCTTTCCT GTTTTTCACT GACATGTGCA TTTACTGGAT TCACAGGTTC 
CTTCACCACA AGTTGATATA TAAGTATTTC CACAAACCAC ATCACGTTTG GAAGATTCCC 
ACACCATTCG CCAGCCATGC TTTCCACCCT GTGGACGGAT TTCTTCAGGG CCTGCCGTAC 
CACATCTACC CCTTCTTCTT TCCTCTGCAC AAGGTTCTGT ACTTAATCCT GTACGTGTTT 
GTCAACATAT GGACCATCTC CATCCACGAC GGGGACTATC GTGTACCCAA CATGGTGGAG 
GAGATCATCA ATGGCTCCGC TCACCACACT GACCACCACC TCTTCTTTGA CTACAACTAC 
GGCCAGTACT TCACCCTGTG GGACCGTATT GGAGGATCCT ACCGCTACCC TTCGGCCTTG 
ATGGGCAAAG GGCCACACGA CCAAATTAAA AAACTGATGG CAGAAGGGAA ACTGATCTCA 
AACAGCTCAA AGGGCCACAC TAACAATAAT CAAAAAAATG GTATTCACGT CAAGAAAGAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Lxr regulates lipid metabolic and visual perception pathways during zebrafish development.
Molecular and cellular endocrinology41929-43(2016 Jan)
Pinto CL,Kalasekar SM,McCollum CW,Riu A,Jonsson P,Lopez J,Swindell EC,Bouhlatouf A,Balaguer P,Bondesson M,Gustafsson JÅ

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

The draft genome of the grass carp (Ctenopharyngodon idellus) provides insights into its evolution and vegetarian adaptation.
Nature genetics47(6)625-31(2015 Jun)
Wang Y,Lu Y,Zhang Y,Ning Z,Li Y,Zhao Q,Lu H,Huang R,Xia X,Feng Q,Liang X,Liu K,Zhang L,Lu T,Huang T,Fan D,Weng Q,Zhu C,Lu Y,Li W,Wen Z,Zhou C,Tian Q,Kang X,Shi M,Zhang W,Jang S,Du F,He S,Liao L,Li Y,Gui B,He H,Ning Z,Yang C,He L,Luo L,Yang R,Luo Q,Liu X,Li S,Huang W,Xiao L,Lin H,Han B,Zhu Z

Interspecies protein-protein interaction network construction for characterization of host-pathogen interactions: a Candida albicans-zebrafish interaction study.
BMC systems biology779(2013 Aug)
Wang YC,Lin C,Chuang MT,Hsieh WP,Lan CY,Chuang YJ,Chen BS

The zebrafish gene map defines ancestral vertebrate chromosomes.
Genome research15(9)1307-14(2005 Sep)
Woods IG,Wilson C,Friedlander B,Chang P,Reyes DK,Nix R,Kelly PD,Chu F,Postlethwait JH,Talbot WS

REQUEST A QUOTE

EMAIL

gene@genscript.com

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

sc5d cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

News & Blogs

REQUEST A QUOTE

EMAIL

PHONE

ONLINE FORM

ONLINE FORM