nup205 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	nup205
Entrez Gene ID	445382
Full Name	nucleoporin 205
Synonyms	zgc:111802
General protein information	Preferred Names nucleoporin 205 Names nuclear pore complex protein Nup205 nucleoporin 205kDa
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021467634.1	XP_021323309.1	nuclear pore complex protein Nup205 isoform X1
XM_009293477.2	XP_009291752.1	nuclear pore complex protein Nup205 isoform X1
NM_001313687.1	NP_001300616.1	nuclear pore complex protein Nup205

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	AT5G51200	NP_199933.5
Gallus gallus (chicken)	NUP205	XP_416176.2
Pan troglodytes (chimpanzee)	NUP205	XP_527899.3
Rattus norvegicus (Norway rat)	Nup205	NP_001102090.1
Drosophila melanogaster (fruit fly)	Nup205	NP_728308.1
Macaca mulatta (Rhesus monkey)	NUP205	XP_001105911.1
Bos taurus (cattle)	NUP205	NP_001124403.1
Mus musculus (house mouse)	Nup205	NP_081789.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008090	XP_317367.4
Danio rerio (zebrafish)	nup205	NP_001003859.1
Xenopus tropicalis (tropical clawed frog)	nup205	NP_001135596.1
Homo sapiens (human)	NUP205	NP_055950.1
Canis lupus familiaris (dog)	NUP205	XP_005629638.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of 315 genes essential for early zebrafish development.
Amsterdam A, Nissen RM, Sun Z, Swindell EC, Farrington S, Hopkins N
Proceedings of the National Academy of Sciences of the United States of America101(35)12792-7(2004 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following nup205 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nup205 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa41178	NM_001313687.1 Latest version!	Danio rerio nucleoporin 205 (nup205), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa41179	XM_009293477.2 Latest version!	Danio rerio nucleoporin 205 (nup205), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa149850	XM_021467634.1 Latest version!	Danio rerio nucleoporin 205 (nup205), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nup205 ( NM_001313687.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001300616.1 Danio rerio nucleoporin 205 (nup205), mRNA.

Price & Availability↑

CloneID	ODa41178
Clone ID Related Accession (Same CDS sequence)	NM_001313687.1
Accession Version	NM_001313687.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5913bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1499184000000
Organism	Danio rerio(zebrafish)
Product	nuclear pore complex protein Nup205
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX005474.6, CR450784.5, DR718726.1 and CN507068.1. On Aug 25, 2015 this sequence version replaced XM_009293478.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGACGC AGATGGCGGT AAATTCAGGA GCCAGTCTGT GGGGGCCGCT GAAGGAGCTC 
TGGGAGGCTG TGGAAGGGGC CATTTGGAGA CGTCAACCAG AAAGTGTTCA TCTTTTAGAT 
CTGCAGTTGA AGAAACACAA ACCCTACTTC CTCAACCTCT TTAAAAACCC ACCGAAGAGT 
GCAGAACAGA GAGAGAAGGT TCGGAAAGCC AGTACAGAAG GCATTGCTAT CCAGGGTCAG 
CAGGGAGCAC GACTACTACC TGAGCAGCTC ATCTCTGAAG CCTTCATCCT TAGTGACCTT 
TTTGACCTTG GAGAACTAGC CGCCCTCGAG CTGCTGCTGG CAGGAGAGCA TCAGCAGCCT 
CATTTCCCCG GCCTGACCCG AGGCCTGGTA GCTGTGCTCC TTTACTGGGA TGGTAAACGC 
TGCGTGGCCA ACTCTCTCCG CTCACTTATC CAGTCCAGAC ATGGGAAGAC TTTCACTCTT 
GACTTGAGTC CTGAATTGGT GAATCTCACC ACACGGTTCA CAGATGAGCT GATGGCCCAG 
GGTTTGACCA AGCAAATACT GAACCTGGTC TCTGACGTCA GCGTGACCCG TGAGTTTGAG 
AGGCTGCAGA AAGAGCGTGG CCTTGGCAAT GAGAAACACA GGAAAGAGGT ATCTGACCTC 
ATTAAAGAGT GCCGACAGTC TCTGGCAGAG TGTTTGTTTG CGTGGGCATG CCAGTCACCG 
CTAGGCAAAG ATGACACGCT GGCTCTTATT GGGCATTTAG AGATGGTGAC CGCTGAAGCT 
GATGGCTCTT TGGATAGTGT CAACCTTGCT TTGGTCATGG CATTGCTCTA TTCCCTGGAT 
GTTAGTTTTC TGGAGCAAGG AACAGAAGAT AGAGAAGACC TGCTCCAGGC TTTGCCTCTG 
TTGACGGAGA AACAGTACGT GGCAGCCGTG CACAGTCGGC TGGTTGAGGG TCAAGGCTGG 
AAGCTTCCAG GACTGCAGGC AGTGGTGCAG CTGGCCTGGG CGCTGTCTCT GAGGGCCCTT 
TCACAACTTC CACAGGGTGC AGCTTTAGTG GAGTTCACAG AAGCAGATGA GGCTCTGGCG 
GACCAGGCAC TGTTGGGAGG CGTCTTCCTC TTTCTGACGG AGGGCGTCCT GGGAAGTGAT 
GGTTTCAGCC AAGAAGAATT TTACACTCGG CGGCTGCATT CTCTGATTAC TGATTTCCTG 
GCACTCATGC CAATGAAGGT GAAACAGTTG CGCAACCGTG CCGATGAAGA TGCTCGTCTG 
ATCCACATGG CTCTGCAGAT GGGCAGCGAG CCGCCATCAT CTCTTCGCAA GGACCTGGAC 
CACCTCATGA TCCTTATTGG AGAATTTTAC AGCAAAGATC CATTTGAACT TGAGCTGGCT 
TTAGAGTTTT GGTGTCCATC AGAGTCTCTT CAGCACACTT CACTCACTGG ATCTTTTCTT 
GGAGTACCTC TCCAAAGACC CCCACATAAA CAGGTGGTTC TCTCCAAGTT TGTGCGTCAG 
ATGGGTGATC TGCTTCCTGC GACTCTTTAC ATCCCATACC TGCGAATGCT GAAAGGACTT 
GCAAATGGCC CTCAGTGCTC CCACTACTGC TTCAGTCTGC TTAAAACTAA CGGCGCTCCA 
CACGGAGAGA ACCGGCAGGC TGGAGTGTCA GGCAGTCTTG TGTCATGGGA ACACTTTTTC 
CACTCGCTCA TGCTGTACCA TGAAAACCTG CGACGAGACG TGCCCAGTGC CGACAGCACA 
CAATACCGTC ATCTACCAAT CAGAGGCATC ACTCAGAGAG AGCTGGAAGG TCTCACTGCA 
TTCCTCCAGC TGCTCACCAC GATCATCACC TGGAGTGAGA ATGCACGTCT GGCTCTTTGT 
GAGCACCCTC AGTGGACCCC TGTGGTGGTG ATGCTGGGTT TGCTACAGTG TAGTGTTCAG 
CCCGTCCTCA AAGCTCAAGT CCTTCATGTT CTCGCTGCTT TTGGCAAATC TCCTGAGATT 
GCAGCCTCCC TCTGGCAGTC TCTAGAGTAC ACACAGATCC TACAAACAGT GAAAATCCCT 
GGTCAGAGAC AGGCTGCTGG GATCGAGGTG GAGCTGAATG AGATTGAATC GAGCTGTGAG 
GAGTATCCAC TCACACGGGC CTTTTGTCAT CTGATCAGTA CTCTGGTGGA GAGCGCACTT 
CCAGTCAATC TTGGAGCAGG ACTGCGAGCT CCCGGCTTCC AACCTTATCT TGACTTCCTG 
CGTGACTCTG TGTTTCTGGC CTTCCCCACG CGAGCCTACC GTCGCTCAGC AGAAAAGTGG 
GAGGTAGCTG AAGCAGTGCT AGAGGTTTTT CACAAACTTC TGCGAGAATA TGAGCCCCAG 
CCTTCAGACT TCCTGCCGGA GATGGTGGAA CTTCAAGGAG AGCAGGTGCC GGCCCATAAG 
CCCCCGGGGC ACAGCCTGAT GTTCCACCTT TTGAACGACT CACCCACCCT CTCTCTCTGC 
CTCAACCTAC TGGAGGAGGG CGCCCGCCAG CTTGACACAT ACGCTCAATT CCCTGGCAAG 
AAGCAGTTAG AGTCTGCAGT TCTGCACTGT CTGTGTTTGC TGGAGCTAGC CCTGCAGAAG 
GAGGTGACGT TCATGGATCT GCTGAGAGAA AGTCAAACAT CACTTCTTGT GTCTCCTTTG 
GAGCAGCTCC TGCAGGGCGT CAGTGCTCAG AGCAGAAAGG CAGATCACAT CACCAACATT 
GCTAGGTACC TGTATCACAG CAGCTCCAAT CCTGATGCTG CGTTCCAGAG TGCAAAGATC 
CTGCGACGGA TCACTCGCTA CCCAAACATC CAGGCCAGAC TGGTTGGAGA CTTCACTCAT 
GATCAGGCAG TGAGTGAGAG GTTGATGGCC GGCTTTGTGG AGTGTCTGGA CAGTGAAGAG 
GCTCAGGAAG GAGTGACCAC AAATGATTCA GACTCTGAGA AACGGGTGGC CAGAATTCGT 
CATGAGACCA AGATCCACAT CTTGAATCTT TTGATCACGT CCCTGGAGCT AACAGGCCCC 
AATCTGGGCC TGTATCTGCT TGGCTATGAA GTCAAGAAGC CAGTTTCCTC CACTAACCTT 
CAAGACCCAG GTGTTCTTGG CTGTCCGCGG AGCTGCCTGC ATGCGATTCT CAGTCTGCTT 
CAGAGGGGCA GTGACTCTCG CTCTGGACCT GGGCTCATTA AACAGGCTCC TCAACTAGCT 
GAACTCTGCT ACCAGGTGAT TTATCAGCTG TGCGCATGTC CTGACACCTC TGGCCCAACC 
ATGAGATACC TCAGGACCAG TCAGGACTTC CTGTTCTCTC ATCTGCAACA CCTACCTTTC 
GTTTTATCAG AAAATGAGAT CGCGGCTCTG TCTCAGATGT CCTGGCTAAT GAAAACCACA 
GCAATCGAGC TCCGAGTGAC CTCACTGAAC CGCCAGCGCT CACACACACA GAGACTCCTG 
AACCTCCTCC TCGATGACCA GCCACACACA TTACACACAG CAGATGGAGA GACCGGCATG 
GAGGAGGAGA ACAGATCAGT CAGTGGTTTT CTGCAGTTTG GCACCGTCTC CAAGGTGCGC 
AGGAGGCTGC TCAGTGTTTT GGATGCCATA GATTTCAGTC AGGAGGCACC AGAGCTTCTG 
CAGCTGGACT TCTTTGAGCG CACTCAGATC GAGCAGGTCA TCACTAACTG TGAACATGTC 
AACGAGCAGG GACACACCGT CTGCAATGTC AAGTTGCTGC ATCGAGTTTT AGTTGCAGAG 
ATCAATGCAC TACAGGGCAT GGCAGCCATC GGGCAAAGAC CACTGCTAAT GGAGGAGGTG 
AATTCAGTCC TGCAGCAGGT GGTGGAGAGG AATCGCGTGC GGCGCAGTCT CAGTGCCAAG 
CGTCATGCTC TGCAGAGCTG GAGGAGTCTG GTGGAGACCA TCCTCACCGC CTGTCCTTCA 
GAACTCATCC CTGCAGACCA GCGGCAGCTC ATCATCAGAG ATCTGCTGCT TGACCTGCAT 
GACAAGGTGT TATCAGAAGA TGCTGCTGGT GAACTGATGC CAATCGTAGC CGGAGCAGTC 
TTCACATTAA CAGCACACCT TAGCCAATCA GTGCTCTCTG AACAGCAGGG GGTGGGGCCA 
GAGGGTGGCT CTGGATCAGG ATTTGCATCC ATTGCTAACT CCGCCCTTCA CCTCATCCTG 
AGGAAGCTGC TGGACTTTAT CTTGTGCACA GGTGGAGGCT TTCAGCGTCT CAGGGCTCAT 
TTGTATGGAG CTCTGCTGTA CTACCTGCAG ATCGCCCAGA AACCTGAGGA GCCAGAGACA 
CTGCAGACAG GCACATCCAT GTGGGAGCGT TTGACTGCCC CTGAAGATTG CTTCTCCAAG 
CTACAGAGAG AAAACCTGTC CATCATCGAG AGCTATGGCA CTGCGCTAAT GGAAGTGGTG 
TGCAGAGACG CTTGTGATGG CCATGAGATC GGCCGAATGC TGGCTCTGGC CGTGTTAGAC 
AGGGTGTTGT CTATAGACAG GCAGTGTCAG TGGCTGGTGT ACTTGTGTAA CAGTGGTTAC 
CTGCGTGTGC TGGTGGAGAG TTTAAAGCAG GATGACGTTT CTTTGCAAAC TCTTCTCACG 
CCTCAGCCGC CGCTGCTTAA ACCGTTGTAC ATCTACGAGT CCAAAATGGC TCTGCTGACC 
CGCGTGGCCA AGACCGCTCA GGGTGCGATG GAGCTGCTGC GCTGTGGTCT GGTCGCTCAG 
CTGGTGGAGT GTCAGGTGTT TCACATGCTC CCTCAGAATG ACGCGCTCAG AGTGTTTGGG 
CAGAGAGATC CTTCAGGGTT TATTCCTAGT CCTCTGGAGC GATACAGGCA GATCCTGCTT 
CCTACACTCA GACTGATGCA GGTCATCTTG ACCTCCACCA CCGCACAGCA CCAGCAGGGG 
GCAGCACAGG TGCTCCAGTG GCTTATCGTT CATTCGGATG TCATTCAGTC TATTCTGCAT 
GGTCAGGACA TGAGCATGGG CTCTTTACAG GAACTGTCTC TGCTCACTGC CATCATCAGC 
AAGACGGCAC TGCCAGGAGC ACTTGAGATG GGACAGGAAA TCAACAGCGC TGCCCTCATG 
GAGTTACAGG GACACATCGG CAGATTCCAG CGGCAGTCGT TGTCTCTGCT GGTGCGTCTG 
GTGGGCACTG ATCGCGCTCG CTACCTTAAG CAGATTGAGG ACACCGTTTC TCCCAGCAAC 
CTAGCAGAAA AAAGAGAGGA AATGGAGGTT GCCATGCAAC AGATCTGTGC TAATATTATG 
GAGTACTGCC AGACGCTGCT GCTGCAGAGC TCATCTGAAG CCCAGTTCTC ACTCTGTCTG 
TTCAGCCCAT CAGCAAGTGA ACCAGCCGAT GTGTCCATCC CCTCTGCCCG CGTGCCCAGT 
CTGGGGCTGG TTCTGCTCCT GCTGAAAAAC AGCGCCAGCG ACTTCTTCAG GTATCACGAC 
AGTCACAGAC AGAGCCTGAA CAAGCTGGAG CGAGTGGAGC AGCTTCCTCC TGAGGAACTC 
AAAGAGCTGT GCCAGGGTTT GGTGTCTGGT TCTGGTGGAG TGGAAAAGAT CTCATCAGTG 
CAGAGAAACG TACTCGCCAA ACGGAGACTC GTCCAGCTGG TCAACAACAG AGCCAAGCTT 
CTTGCCCTCT GCTCTTATAT CATTGAGACG TGTCTGTTTG TCATCTGGCG CCACCTAGAG 
TTTTACTTGC TTTACTGCAC TCCCACTGAC CCCAAAGACT CACTGCTGCC AGGTTACAGA 
GATCCTGCAG GCAGCAGAGG ATTGAGCGTG TCGAGAGTGA GCCAGCAAGA TCTTGAACAG 
TTGCAGAGTG ACATGTCGAG CAGCTTCAGT GAGCCGCTGC AGAGGAAGCT GCTGGAGGTG 
GAGGGACTTT ATAGCAAAAG CCGCTCTCGT CACACCTTCA TCCAGGCCCT GACACGCAGG 
ATCCGCGGCC TCGTACACCA CGCCAAAAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300616.1
CDS	19..5931
Translation	MATQMAVNSGASLWGPLKELWEAVEGAIWRRQPESVHLLDLQLKKHKPYFLNLFKNPPKSAEQREKVRKASTEGIAIQGQQGARLLPEQLISEAFILSDLFDLGELAALELLLAGEHQQPHFPGLTRGLVAVLLYWDGKRCVANSLRSLIQSRHGKTFTLDLSPELVNLTTRFTDELMAQGLTKQILNLVSDVSVTREFERLQKERGLGNEKHRKEVSDLIKECRQSLAECLFAWACQSPLGKDDTLALIGHLEMVTAEADGSLDSVNLALVMALLYSLDVSFLEQGTEDREDLLQALPLLTEKQYVAAVHSRLVEGQGWKLPGLQAVVQLAWALSLRALSQLPQGAALVEFTEADEALADQALLGGVFLFLTEGVLGSDGFSQEEFYTRRLHSLITDFLALMPMKVKQLRNRADEDARLIHMALQMGSEPPSSLRKDLDHLMILIGEFYSKDPFELELALEFWCPSESLQHTSLTGSFLGVPLQRPPHKQVVLSKFVRQMGDLLPATLYIPYLRMLKGLANGPQCSHYCFSLLKTNGAPHGENRQAGVSGSLVSWEHFFHSLMLYHENLRRDVPSADSTQYRHLPIRGITQRELEGLTAFLQLLTTIITWSENARLALCEHPQWTPVVVMLGLLQCSVQPVLKAQVLHVLAAFGKSPEIAASLWQSLEYTQILQTVKIPGQRQAAGIEVELNEIESSCEEYPLTRAFCHLISTLVESALPVNLGAGLRAPGFQPYLDFLRDSVFLAFPTRAYRRSAEKWEVAEAVLEVFHKLLREYEPQPSDFLPEMVELQGEQVPAHKPPGHSLMFHLLNDSPTLSLCLNLLEEGARQLDTYAQFPGKKQLESAVLHCLCLLELALQKEVTFMDLLRESQTSLLVSPLEQLLQGVSAQSRKADHITNIARYLYHSSSNPDAAFQSAKILRRITRYPNIQARLVGDFTHDQAVSERLMAGFVECLDSEEAQEGVTTNDSDSEKRVARIRHETKIHILNLLITSLELTGPNLGLYLLGYEVKKPVSSTNLQDPGVLGCPRSCLHAILSLLQRGSDSRSGPGLIKQAPQLAELCYQVIYQLCACPDTSGPTMRYLRTSQDFLFSHLQHLPFVLSENEIAALSQMSWLMKTTAIELRVTSLNRQRSHTQRLLNLLLDDQPHTLHTADGETGMEEENRSVSGFLQFGTVSKVRRRLLSVLDAIDFSQEAPELLQLDFFERTQIEQVITNCEHVNEQGHTVCNVKLLHRVLVAEINALQGMAAIGQRPLLMEEVNSVLQQVVERNRVRRSLSAKRHALQSWRSLVETILTACPSELIPADQRQLIIRDLLLDLHDKVLSEDAAGELMPIVAGAVFTLTAHLSQSVLSEQQGVGPEGGSGSGFASIANSALHLILRKLLDFILCTGGGFQRLRAHLYGALLYYLQIAQKPEEPETLQTGTSMWERLTAPEDCFSKLQRENLSIIESYGTALMEVVCRDACDGHEIGRMLALAVLDRVLSIDRQCQWLVYLCNSGYLRVLVESLKQDDVSLQTLLTPQPPLLKPLYIYESKMALLTRVAKTAQGAMELLRCGLVAQLVECQVFHMLPQNDALRVFGQRDPSGFIPSPLERYRQILLPTLRLMQVILTSTTAQHQQGAAQVLQWLIVHSDVIQSILHGQDMSMGSLQELSLLTAIISKTALPGALEMGQEINSAALMELQGHIGRFQRQSLSLLVRLVGTDRARYLKQIEDTVSPSNLAEKREEMEVAMQQICANIMEYCQTLLLQSSSEAQFSLCLFSPSASEPADVSIPSARVPSLGLVLLLLKNSASDFFRYHDSHRQSLNKLERVEQLPPEELKELCQGLVSGSGGVEKISSVQRNVLAKRRLVQLVNNRAKLLALCSYIIETCLFVIWRHLEFYLLYCTPTDPKDSLLPGYRDPAGSRGLSVSRVSQQDLEQLQSDMSSSFSEPLQRKLLEVEGLYSKSRSRHTFIQALTRRIRGLVHHAKS

Target ORF information:

RefSeq Version	NM_001313687.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nucleoporin 205 (nup205), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313687.1

ORF Insert Sequence:

ATGGCGACGC AGATGGCGGT AAATTCAGGA GCCAGTCTGT GGGGGCCGCT GAAGGAGCTC 
TGGGAGGCTG TGGAAGGGGC CATTTGGAGA CGTCAACCAG AAAGTGTTCA TCTTTTAGAT 
CTGCAGTTGA AGAAACACAA ACCCTACTTC CTCAACCTCT TTAAAAACCC ACCGAAGAGT 
GCAGAACAGA GAGAGAAGGT TCGGAAAGCC AGTACAGAAG GCATTGCTAT CCAGGGTCAG 
CAGGGAGCAC GACTACTACC TGAGCAGCTC ATCTCTGAAG CCTTCATCCT TAGTGACCTT 
TTTGACCTTG GAGAACTAGC CGCCCTCGAG CTGCTGCTGG CAGGAGAGCA TCAGCAGCCT 
CATTTCCCCG GCCTGACCCG AGGCCTGGTA GCTGTGCTCC TTTACTGGGA TGGTAAACGC 
TGCGTGGCCA ACTCTCTCCG CTCACTTATC CAGTCCAGAC ATGGGAAGAC TTTCACTCTT 
GACTTGAGTC CTGAATTGGT GAATCTCACC ACACGGTTCA CAGATGAGCT GATGGCCCAG 
GGTTTGACCA AGCAAATACT GAACCTGGTC TCTGACGTCA GCGTGACCCG TGAGTTTGAG 
AGGCTGCAGA AAGAGCGTGG CCTTGGCAAT GAGAAACACA GGAAAGAGGT ATCTGACCTC 
ATTAAAGAGT GCCGACAGTC TCTGGCAGAG TGTTTGTTTG CGTGGGCATG CCAGTCACCG 
CTAGGCAAAG ATGACACGCT GGCTCTTATT GGGCATTTAG AGATGGTGAC CGCTGAAGCT 
GATGGCTCTT TGGATAGTGT CAACCTTGCT TTGGTCATGG CATTGCTCTA TTCCCTGGAT 
GTTAGTTTTC TGGAGCAAGG AACAGAAGAT AGAGAAGACC TGCTCCAGGC TTTGCCTCTG 
TTGACGGAGA AACAGTACGT GGCAGCCGTG CACAGTCGGC TGGTTGAGGG TCAAGGCTGG 
AAGCTTCCAG GACTGCAGGC AGTGGTGCAG CTGGCCTGGG CGCTGTCTCT GAGGGCCCTT 
TCACAACTTC CACAGGGTGC AGCTTTAGTG GAGTTCACAG AAGCAGATGA GGCTCTGGCG 
GACCAGGCAC TGTTGGGAGG CGTCTTCCTC TTTCTGACGG AGGGCGTCCT GGGAAGTGAT 
GGTTTCAGCC AAGAAGAATT TTACACTCGG CGGCTGCATT CTCTGATTAC TGATTTCCTG 
GCACTCATGC CAATGAAGGT GAAACAGTTG CGCAACCGTG CCGATGAAGA TGCTCGTCTG 
ATCCACATGG CTCTGCAGAT GGGCAGCGAG CCGCCATCAT CTCTTCGCAA GGACCTGGAC 
CACCTCATGA TCCTTATTGG AGAATTTTAC AGCAAAGATC CATTTGAACT TGAGCTGGCT 
TTAGAGTTTT GGTGTCCATC AGAGTCTCTT CAGCACACTT CACTCACTGG ATCTTTTCTT 
GGAGTACCTC TCCAAAGACC CCCACATAAA CAGGTGGTTC TCTCCAAGTT TGTGCGTCAG 
ATGGGTGATC TGCTTCCTGC GACTCTTTAC ATCCCATACC TGCGAATGCT GAAAGGACTT 
GCAAATGGCC CTCAGTGCTC CCACTACTGC TTCAGTCTGC TTAAAACTAA CGGCGCTCCA 
CACGGAGAGA ACCGGCAGGC TGGAGTGTCA GGCAGTCTTG TGTCATGGGA ACACTTTTTC 
CACTCGCTCA TGCTGTACCA TGAAAACCTG CGACGAGACG TGCCCAGTGC CGACAGCACA 
CAATACCGTC ATCTACCAAT CAGAGGCATC ACTCAGAGAG AGCTGGAAGG TCTCACTGCA 
TTCCTCCAGC TGCTCACCAC GATCATCACC TGGAGTGAGA ATGCACGTCT GGCTCTTTGT 
GAGCACCCTC AGTGGACCCC TGTGGTGGTG ATGCTGGGTT TGCTACAGTG TAGTGTTCAG 
CCCGTCCTCA AAGCTCAAGT CCTTCATGTT CTCGCTGCTT TTGGCAAATC TCCTGAGATT 
GCAGCCTCCC TCTGGCAGTC TCTAGAGTAC ACACAGATCC TACAAACAGT GAAAATCCCT 
GGTCAGAGAC AGGCTGCTGG GATCGAGGTG GAGCTGAATG AGATTGAATC GAGCTGTGAG 
GAGTATCCAC TCACACGGGC CTTTTGTCAT CTGATCAGTA CTCTGGTGGA GAGCGCACTT 
CCAGTCAATC TTGGAGCAGG ACTGCGAGCT CCCGGCTTCC AACCTTATCT TGACTTCCTG 
CGTGACTCTG TGTTTCTGGC CTTCCCCACG CGAGCCTACC GTCGCTCAGC AGAAAAGTGG 
GAGGTAGCTG AAGCAGTGCT AGAGGTTTTT CACAAACTTC TGCGAGAATA TGAGCCCCAG 
CCTTCAGACT TCCTGCCGGA GATGGTGGAA CTTCAAGGAG AGCAGGTGCC GGCCCATAAG 
CCCCCGGGGC ACAGCCTGAT GTTCCACCTT TTGAACGACT CACCCACCCT CTCTCTCTGC 
CTCAACCTAC TGGAGGAGGG CGCCCGCCAG CTTGACACAT ACGCTCAATT CCCTGGCAAG 
AAGCAGTTAG AGTCTGCAGT TCTGCACTGT CTGTGTTTGC TGGAGCTAGC CCTGCAGAAG 
GAGGTGACGT TCATGGATCT GCTGAGAGAA AGTCAAACAT CACTTCTTGT GTCTCCTTTG 
GAGCAGCTCC TGCAGGGCGT CAGTGCTCAG AGCAGAAAGG CAGATCACAT CACCAACATT 
GCTAGGTACC TGTATCACAG CAGCTCCAAT CCTGATGCTG CGTTCCAGAG TGCAAAGATC 
CTGCGACGGA TCACTCGCTA CCCAAACATC CAGGCCAGAC TGGTTGGAGA CTTCACTCAT 
GATCAGGCAG TGAGTGAGAG GTTGATGGCC GGCTTTGTGG AGTGTCTGGA CAGTGAAGAG 
GCTCAGGAAG GAGTGACCAC AAATGATTCA GACTCTGAGA AACGGGTGGC CAGAATTCGT 
CATGAGACCA AGATCCACAT CTTGAATCTT TTGATCACGT CCCTGGAGCT AACAGGCCCC 
AATCTGGGCC TGTATCTGCT TGGCTATGAA GTCAAGAAGC CAGTTTCCTC CACTAACCTT 
CAAGACCCAG GTGTTCTTGG CTGTCCGCGG AGCTGCCTGC ATGCGATTCT CAGTCTGCTT 
CAGAGGGGCA GTGACTCTCG CTCTGGACCT GGGCTCATTA AACAGGCTCC TCAACTAGCT 
GAACTCTGCT ACCAGGTGAT TTATCAGCTG TGCGCATGTC CTGACACCTC TGGCCCAACC 
ATGAGATACC TCAGGACCAG TCAGGACTTC CTGTTCTCTC ATCTGCAACA CCTACCTTTC 
GTTTTATCAG AAAATGAGAT CGCGGCTCTG TCTCAGATGT CCTGGCTAAT GAAAACCACA 
GCAATCGAGC TCCGAGTGAC CTCACTGAAC CGCCAGCGCT CACACACACA GAGACTCCTG 
AACCTCCTCC TCGATGACCA GCCACACACA TTACACACAG CAGATGGAGA GACCGGCATG 
GAGGAGGAGA ACAGATCAGT CAGTGGTTTT CTGCAGTTTG GCACCGTCTC CAAGGTGCGC 
AGGAGGCTGC TCAGTGTTTT GGATGCCATA GATTTCAGTC AGGAGGCACC AGAGCTTCTG 
CAGCTGGACT TCTTTGAGCG CACTCAGATC GAGCAGGTCA TCACTAACTG TGAACATGTC 
AACGAGCAGG GACACACCGT CTGCAATGTC AAGTTGCTGC ATCGAGTTTT AGTTGCAGAG 
ATCAATGCAC TACAGGGCAT GGCAGCCATC GGGCAAAGAC CACTGCTAAT GGAGGAGGTG 
AATTCAGTCC TGCAGCAGGT GGTGGAGAGG AATCGCGTGC GGCGCAGTCT CAGTGCCAAG 
CGTCATGCTC TGCAGAGCTG GAGGAGTCTG GTGGAGACCA TCCTCACCGC CTGTCCTTCA 
GAACTCATCC CTGCAGACCA GCGGCAGCTC ATCATCAGAG ATCTGCTGCT TGACCTGCAT 
GACAAGGTGT TATCAGAAGA TGCTGCTGGT GAACTGATGC CAATCGTAGC CGGAGCAGTC 
TTCACATTAA CAGCACACCT TAGCCAATCA GTGCTCTCTG AACAGCAGGG GGTGGGGCCA 
GAGGGTGGCT CTGGATCAGG ATTTGCATCC ATTGCTAACT CCGCCCTTCA CCTCATCCTG 
AGGAAGCTGC TGGACTTTAT CTTGTGCACA GGTGGAGGCT TTCAGCGTCT CAGGGCTCAT 
TTGTATGGAG CTCTGCTGTA CTACCTGCAG ATCGCCCAGA AACCTGAGGA GCCAGAGACA 
CTGCAGACAG GCACATCCAT GTGGGAGCGT TTGACTGCCC CTGAAGATTG CTTCTCCAAG 
CTACAGAGAG AAAACCTGTC CATCATCGAG AGCTATGGCA CTGCGCTAAT GGAAGTGGTG 
TGCAGAGACG CTTGTGATGG CCATGAGATC GGCCGAATGC TGGCTCTGGC CGTGTTAGAC 
AGGGTGTTGT CTATAGACAG GCAGTGTCAG TGGCTGGTGT ACTTGTGTAA CAGTGGTTAC 
CTGCGTGTGC TGGTGGAGAG TTTAAAGCAG GATGACGTTT CTTTGCAAAC TCTTCTCACG 
CCTCAGCCGC CGCTGCTTAA ACCGTTGTAC ATCTACGAGT CCAAAATGGC TCTGCTGACC 
CGCGTGGCCA AGACCGCTCA GGGTGCGATG GAGCTGCTGC GCTGTGGTCT GGTCGCTCAG 
CTGGTGGAGT GTCAGGTGTT TCACATGCTC CCTCAGAATG ACGCGCTCAG AGTGTTTGGG 
CAGAGAGATC CTTCAGGGTT TATTCCTAGT CCTCTGGAGC GATACAGGCA GATCCTGCTT 
CCTACACTCA GACTGATGCA GGTCATCTTG ACCTCCACCA CCGCACAGCA CCAGCAGGGG 
GCAGCACAGG TGCTCCAGTG GCTTATCGTT CATTCGGATG TCATTCAGTC TATTCTGCAT 
GGTCAGGACA TGAGCATGGG CTCTTTACAG GAACTGTCTC TGCTCACTGC CATCATCAGC 
AAGACGGCAC TGCCAGGAGC ACTTGAGATG GGACAGGAAA TCAACAGCGC TGCCCTCATG 
GAGTTACAGG GACACATCGG CAGATTCCAG CGGCAGTCGT TGTCTCTGCT GGTGCGTCTG 
GTGGGCACTG ATCGCGCTCG CTACCTTAAG CAGATTGAGG ACACCGTTTC TCCCAGCAAC 
CTAGCAGAAA AAAGAGAGGA AATGGAGGTT GCCATGCAAC AGATCTGTGC TAATATTATG 
GAGTACTGCC AGACGCTGCT GCTGCAGAGC TCATCTGAAG CCCAGTTCTC ACTCTGTCTG 
TTCAGCCCAT CAGCAAGTGA ACCAGCCGAT GTGTCCATCC CCTCTGCCCG CGTGCCCAGT 
CTGGGGCTGG TTCTGCTCCT GCTGAAAAAC AGCGCCAGCG ACTTCTTCAG GTATCACGAC 
AGTCACAGAC AGAGCCTGAA CAAGCTGGAG CGAGTGGAGC AGCTTCCTCC TGAGGAACTC 
AAAGAGCTGT GCCAGGGTTT GGTGTCTGGT TCTGGTGGAG TGGAAAAGAT CTCATCAGTG 
CAGAGAAACG TACTCGCCAA ACGGAGACTC GTCCAGCTGG TCAACAACAG AGCCAAGCTT 
CTTGCCCTCT GCTCTTATAT CATTGAGACG TGTCTGTTTG TCATCTGGCG CCACCTAGAG 
TTTTACTTGC TTTACTGCAC TCCCACTGAC CCCAAAGACT CACTGCTGCC AGGTTACAGA 
GATCCTGCAG GCAGCAGAGG ATTGAGCGTG TCGAGAGTGA GCCAGCAAGA TCTTGAACAG 
TTGCAGAGTG ACATGTCGAG CAGCTTCAGT GAGCCGCTGC AGAGGAAGCT GCTGGAGGTG 
GAGGGACTTT ATAGCAAAAG CCGCTCTCGT CACACCTTCA TCCAGGCCCT GACACGCAGG 
ATCCGCGGCC TCGTACACCA CGCCAAAAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nup205 ( XM_009293477.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009291752.1 Danio rerio nucleoporin 205 (nup205), transcript variant X1, mRNA.

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CloneID	ODa41179
Clone ID Related Accession (Same CDS sequence)	XM_009293477.2
Accession Version	XM_009293477.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	nuclear pore complex protein Nup205 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293477.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACGC AGATGGCGGT AAATTCAGGA GCCAGTCTGT GGGGGCCGCT GAAGGAGCTC 
TGGGAGGCTG TGGAAGGGGC CATTTGGAGA CGTCAACCAG AAAGTGTTCA TCTTTTAGAT 
CTGCAGTTGA AGAAACACAA ACCCTACTTC CTCAACCTCT TTAAAAACCC ACCGAAGAGT 
GCAGAACAGA GAGAGAAGGT TCGGAAAGCC AGTACAGAAG GCATTGCTAT CCAGGGTCAG 
CAGGGAGCAC GACTACTACC TGAGCAGCTC ATCTCTGAAG CCTTCATCCT TAGTGACCTT 
TTTGACCTTG GAGAACTAGC CGCCCTCGAG CTGCTGCTGG CAGGAGAGCA TCAGCAGCCT 
CATTTCCCCG GCCTGACCCG AGGCCTGGTA GCTGTGCTCC TTTACTGGGA TGGTAAACGC 
TGCGTGGCCA ACTCTCTCCG CTCACTTATC CAGTCCAGAC ATGGGAAGAC TTTCACTCTT 
GACTTGAGTC CTGAATTGGT GAATCTCACC ACACGGTTCA CAGATGAGCT GATGGCCCAG 
GGTTTGACCA AGCAAATACT GAACCTGGTC TCTGACGTCA GCGTGACCCG TGAGTTTGAG 
AGGCTGCAGA AAGAGCGTGG CCTTGGCAAT GAGAAACACA GGAAAGAGGT ATCTGACCTC 
ATTAAAGAGT GCCGACAGTC TCTGGCAGAG TGTTTGTTTG CGTGGGCATG CCAGTCACCG 
CTAGGCAAAG ATGACACGCT GGCTCTTATT GGGCATTTAG AGATGGTGAC CGCTGAAGCT 
GATGGCTCTT TGGATAGTGT CAACCTTGCT TTGGTCATGG CATTGCTCTA TTCCCTGGAT 
GTTAGTTTTC TGGAGCAAGG AACAGAAGAT AGAGAAGACC TGCTCCAGGC TTTGCCTCTG 
TTGACGGAGA AACAGTACGT GGCAGCCGTG CACAGTCGGC TGGTTGAGGG TCAAGGCTGG 
AAGCTTCCAG GACTGCAGGC AGTGGTGCAG CTGGCCTGGG CGCTGTCTCT GAGGGCCCTT 
TCACAACTTC CACAGGGTGC AGCTTTAGTG GAGTTCACAG AAGCAGATGA GGCTCTGGCG 
GACCAGGCAC TGTTGGGAGG CGTCTTCCTC TTTCTGACGG AGGGCGTCCT GGGAAGTGAT 
GGTTTCAGCC AAGAAGAATT TTACACTCGG CGGCTGCATT CTCTGATTAC TGATTTCCTG 
GCACTCATGC CAATGAAGGT GAAACAGTTG CGCAACCGTG CCGATGAAGA TGCTCGTCTG 
ATCCACATGG CTCTGCAGAT GGGCAGCGAG CCGCCATCAT CTCTTCGCAA GGACCTGGAC 
CACCTCATGA TCCTTATTGG AGAATTTTAC AGCAAAGATC CATTTGAACT TGAGCTGGCT 
TTAGAGTTTT GGTGTCCATC AGAGTCTCTT CAGCACACTT CACTCACTGG ATCTTTTCTT 
GGAGTACCTC TCCAAAGACC CCCACATAAA CAGGTGGTTC TCTCCAAGTT TGTGCGTCAG 
ATGGGTGATC TGCTTCCTGC GACTCTTTAC ATCCCATACC TGCGAATGCT GAAAGGACTT 
GCAAATGGCC CTCAGTGCTC CCACTACTGC TTCAGTCTGC TTAAAACTAA CGGCGCTCCA 
CACGGAGAGA ACCGGCAGGC TGGAGTGTCA GGCAGTCTTG TGTCATGGGA ACACTTTTTC 
CACTCGCTCA TGCTGTACCA TGAAAACCTG CGACGAGACG TGCCCAGTGC CGACAGCACA 
CAATACCGTC ATCTACCAAT CAGAGGCATC ACTCAGAGAG AGCTGGAAGG TCTCACTGCA 
TTCCTCCAGC TGCTCACCAC GATCATCACC TGGAGTGAGA ATGCACGTCT GGCTCTTTGT 
GAGCACCCTC AGTGGACCCC TGTGGTGGTG ATGCTGGGTT TGCTACAGTG TAGTGTTCAG 
CCCGTCCTCA AAGCTCAAGT CCTTCATGTT CTCGCTGCTT TTGGCAAATC TCCTGAGATT 
GCAGCCTCCC TCTGGCAGTC TCTAGAGTAC ACACAGATCC TACAAACAGT GAAAATCCCT 
GGTCAGAGAC AGGCTGCTGG GATCGAGGTG GAGCTGAATG AGATTGAATC GAGCTGTGAG 
GAGTATCCAC TCACACGGGC CTTTTGTCAT CTGATCAGTA CTCTGGTGGA GAGCGCACTT 
CCAGTCAATC TTGGAGCAGG ACTGCGAGCT CCCGGCTTCC AACCTTATCT TGACTTCCTG 
CGTGACTCTG TGTTTCTGGC CTTCCCCACG CGAGCCTACC GTCGCTCAGC AGAAAAGTGG 
GAGGTAGCTG AAGCAGTGCT AGAGGTTTTT CACAAACTTC TGCGAGAATA TGAGCCCCAG 
CCTTCAGACT TCCTGCCGGA GATGGTGGAA CTTCAAGGAG AGCAGGTGCC GGCCCATAAG 
CCCCCGGGGC ACAGCCTGAT GTTCCACCTT TTGAACGACT CACCCACCCT CTCTCTCTGC 
CTCAACCTAC TGGAGGAGGG CGCCCGCCAG CTTGACACAT ACGCTCAATT CCCTGGCAAG 
AAGCAGTTAG AGTCTGCAGT TCTGCACTGT CTGTGTTTGC TGGAGCTAGC CCTGCAGAAG 
GAGGTGACGT TCATGGATCT GCTGAGAGAA AGTCAAACAT CACTTCTTGT GTCTCCTTTG 
GAGCAGCTCC TGCAGGGCGT CAGTGCTCAG AGCAGAAAGG CAGATCACAT CACCAACATT 
GCTAGGTACC TGTATCACAG CAGCTCCAAT CCTGATGCTG CGTTCCAGAG TGCAAAGATC 
CTGCGACGGA TCACTCGCTA CCCAAACATC CAGGCCAGAC TGGTTGGAGA CTTCACTCAT 
GATCAGGCAG TGAGTGAGAG GTTGATGGCC GGCTTTGTGG AGTGTCTGGA CAGTGAAGAG 
GCTCAGGAAG GAGTGACCAC AAATGATTCA GACTCTGAGA AACGGGTGGC CAGAATTCGT 
CATGAGACCA AGATCCACAT CTTGAATCTT TTGATCACGT CCCTGGAGCT AACAGGCCCC 
AATCTGGGCC TGTATCTGCT TGGCTATGAA GTCAAGAAGC CAGTTTCCTC CACTAACCTT 
CAAGACCCAG GTGTTCTTGG CTGTCCGCGG AGCTGCCTGC ATGCGATTCT CAGTCTGCTT 
CAGAGGGGCA GTGACTCTCG CTCTGGACCT GGGCTCATTA AACAGGCTCC TCAACTAGCT 
GAACTCTGCT ACCAGGTGAT TTATCAGCTG TGCGCATGTC CTGACACCTC TGGCCCAACC 
ATGAGATACC TCAGGACCAG TCAGGACTTC CTGTTCTCTC ATCTGCAACA CCTACCTTTC 
GTTTTATCAG AAAATGAGAT CGCGGCTCTG TCTCAGATGT CCTGGCTAAT GAAAACCACA 
GCAATCGAGC TCCGAGTGAC CTCACTGAAC CGCCAGCGCT CACACACACA GAGACTCCTG 
AACCTCCTCC TCGATGACCA GCCACACACA TTACACACAG CAGATGGAGA GACCGGCATG 
GAGGAGGAGA ACAGATCAGT CAGTGGTTTT CTGCAGTTTG GCACCGTCTC CAAGGTGCGC 
AGGAGGCTGC TCAGTGTTTT GGATGCCATA GATTTCAGTC AGGAGGCACC AGAGCTTCTG 
CAGCTGGACT TCTTTGAGCG CACTCAGATC GAGCAGGTCA TCACTAACTG TGAACATGTC 
AACGAGCAGG GACACACCGT CTGCAATGTC AAGTTGCTGC ATCGAGTTTT AGTTGCAGAG 
ATCAATGCAC TACAGGGCAT GGCAGCCATC GGGCAAAGAC CACTGCTAAT GGAGGAGGTG 
AATTCAGTCC TGCAGCAGGT GGTGGAGAGG AATCGCGTGC GGCGCAGTCT CAGTGCCAAG 
CGTCATGCTC TGCAGAGCTG GAGGAGTCTG GTGGAGACCA TCCTCACCGC CTGTCCTTCA 
GAACTCATCC CTGCAGACCA GCGGCAGCTC ATCATCAGAG ATCTGCTGCT TGACCTGCAT 
GACAAGGTGT TATCAGAAGA TGCTGCTGGT GAACTGATGC CAATCGTAGC CGGAGCAGTC 
TTCACATTAA CAGCACACCT TAGCCAATCA GTGCTCTCTG AACAGCAGGG GGTGGGGCCA 
GAGGGTGGCT CTGGATCAGG ATTTGCATCC ATTGCTAACT CCGCCCTTCA CCTCATCCTG 
AGGAAGCTGC TGGACTTTAT CTTGTGCACA GGTGGAGGCT TTCAGCGTCT CAGGGCTCAT 
TTGTATGGAG CTCTGCTGTA CTACCTGCAG ATCGCCCAGA AACCTGAGGA GCCAGAGACA 
CTGCAGACAG GCACATCCAT GTGGGAGCGT TTGACTGCCC CTGAAGATTG CTTCTCCAAG 
CTACAGAGAG AAAACCTGTC CATCATCGAG AGCTATGGCA CTGCGCTAAT GGAAGTGGTG 
TGCAGAGACG CTTGTGATGG CCATGAGATC GGCCGAATGC TGGCTCTGGC CGTGTTAGAC 
AGGGTGTTGT CTATAGACAG GCAGTGTCAG TGGCTGGTGT ACTTGTGTAA CAGTGGTTAC 
CTGCGTGTGC TGGTGGAGAG TTTAAAGCAG GATGACGTTT CTTTGCAAAC TCTTCTCACG 
CCTCAGCCGC CGCTGCTTAA ACCGTTGTAC ATCTACGAGT CCAAAATGGC TCTGCTGACC 
CGCGTGGCCA AGACCGCTCA GGGTGCGATG GAGCTGCTGC GCTGTGGTCT GGTCGCTCAG 
CTGGTGGAGT GTCAGGTGTT TCACATGCTC CCTCAGAATG ACGCGCTCAG AGTGTTTGGG 
CAGAGAGATC CTTCAGGGTT TATTCCTAGT CCTCTGGAGC GATACAGGCA GATCCTGCTT 
CCTACACTCA GACTGATGCA GGTCATCTTG ACCTCCACCA CCGCACAGCA CCAGCAGGGG 
GCAGCACAGG TGCTCCAGTG GCTTATCGTT CATTCGGATG TCATTCAGTC TATTCTGCAT 
GGTCAGGACA TGAGCATGGG CTCTTTACAG GAACTGTCTC TGCTCACTGC CATCATCAGC 
AAGACGGCAC TGCCAGGAGC ACTTGAGATG GGACAGGAAA TCAACAGCGC TGCCCTCATG 
GAGTTACAGG GACACATCGG CAGATTCCAG CGGCAGTCGT TGTCTCTGCT GGTGCGTCTG 
GTGGGCACTG ATCGCGCTCG CTACCTTAAG CAGATTGAGG ACACCGTTTC TCCCAGCAAC 
CTAGCAGAAA AAAGAGAGGA AATGGAGGTT GCCATGCAAC AGATCTGTGC TAATATTATG 
GAGTACTGCC AGACGCTGCT GCTGCAGAGC TCATCTGAAG CCCAGTTCTC ACTCTGTCTG 
TTCAGCCCAT CAGCAAGTGA ACCAGCCGAT GTGTCCATCC CCTCTGCCCG CGTGCCCAGT 
CTGGGGCTGG TTCTGCTCCT GCTGAAAAAC AGCGCCAGCG ACTTCTTCAG GTATCACGAC 
AGTCACAGAC AGAGCCTGAA CAAGCTGGAG CGAGTGGAGC AGCTTCCTCC TGAGGAACTC 
AAAGAGCTGT GCCAGGGTTT GGTGTCTGGT TCTGGTGGAG TGGAAAAGAT CTCATCAGTG 
CAGAGAAACG TACTCGCCAA ACGGAGACTC GTCCAGCTGG TCAACAACAG AGCCAAGCTT 
CTTGCCCTCT GCTCTTATAT CATTGAGACG TGTCTGTTTG TCATCTGGCG CCACCTAGAG 
TTTTACTTGC TTTACTGCAC TCCCACTGAC CCCAAAGACT CACTGCTGCC AGGTTACAGA 
GGTCAGCTTT CTCTCAAGCT TCAGGATTAT TTTCAGATGC TGTTGAATGA CATGGTTTCT 
TACCAGATTG CTCTCTCTGC AGATCCTGCA GGCAGCAGAG GATTGAGCGT GTCGAGAGTG 
AGCCAGCAAG ATCTTGAACA GTTGCAGAGT GACATGTCGA GCAGCTTCAG TGAGCCGCTG 
CAGAGGAAGC TGCTGGAGGT GGAGGGACTT TATAGCAAAA GCCGCTCTCG TCACACCTTC 
ATCCAGGCCC TGACACGCAG GATCCGCGGC CTCGTACACC ACGCCAAAAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291752.1
CDS	26..6019
Translation	MATQMAVNSGASLWGPLKELWEAVEGAIWRRQPESVHLLDLQLKKHKPYFLNLFKNPPKSAEQREKVRKASTEGIAIQGQQGARLLPEQLISEAFILSDLFDLGELAALELLLAGEHQQPHFPGLTRGLVAVLLYWDGKRCVANSLRSLIQSRHGKTFTLDLSPELVNLTTRFTDELMAQGLTKQILNLVSDVSVTREFERLQKERGLGNEKHRKEVSDLIKECRQSLAECLFAWACQSPLGKDDTLALIGHLEMVTAEADGSLDSVNLALVMALLYSLDVSFLEQGTEDREDLLQALPLLTEKQYVAAVHSRLVEGQGWKLPGLQAVVQLAWALSLRALSQLPQGAALVEFTEADEALADQALLGGVFLFLTEGVLGSDGFSQEEFYTRRLHSLITDFLALMPMKVKQLRNRADEDARLIHMALQMGSEPPSSLRKDLDHLMILIGEFYSKDPFELELALEFWCPSESLQHTSLTGSFLGVPLQRPPHKQVVLSKFVRQMGDLLPATLYIPYLRMLKGLANGPQCSHYCFSLLKTNGAPHGENRQAGVSGSLVSWEHFFHSLMLYHENLRRDVPSADSTQYRHLPIRGITQRELEGLTAFLQLLTTIITWSENARLALCEHPQWTPVVVMLGLLQCSVQPVLKAQVLHVLAAFGKSPEIAASLWQSLEYTQILQTVKIPGQRQAAGIEVELNEIESSCEEYPLTRAFCHLISTLVESALPVNLGAGLRAPGFQPYLDFLRDSVFLAFPTRAYRRSAEKWEVAEAVLEVFHKLLREYEPQPSDFLPEMVELQGEQVPAHKPPGHSLMFHLLNDSPTLSLCLNLLEEGARQLDTYAQFPGKKQLESAVLHCLCLLELALQKEVTFMDLLRESQTSLLVSPLEQLLQGVSAQSRKADHITNIARYLYHSSSNPDAAFQSAKILRRITRYPNIQARLVGDFTHDQAVSERLMAGFVECLDSEEAQEGVTTNDSDSEKRVARIRHETKIHILNLLITSLELTGPNLGLYLLGYEVKKPVSSTNLQDPGVLGCPRSCLHAILSLLQRGSDSRSGPGLIKQAPQLAELCYQVIYQLCACPDTSGPTMRYLRTSQDFLFSHLQHLPFVLSENEIAALSQMSWLMKTTAIELRVTSLNRQRSHTQRLLNLLLDDQPHTLHTADGETGMEEENRSVSGFLQFGTVSKVRRRLLSVLDAIDFSQEAPELLQLDFFERTQIEQVITNCEHVNEQGHTVCNVKLLHRVLVAEINALQGMAAIGQRPLLMEEVNSVLQQVVERNRVRRSLSAKRHALQSWRSLVETILTACPSELIPADQRQLIIRDLLLDLHDKVLSEDAAGELMPIVAGAVFTLTAHLSQSVLSEQQGVGPEGGSGSGFASIANSALHLILRKLLDFILCTGGGFQRLRAHLYGALLYYLQIAQKPEEPETLQTGTSMWERLTAPEDCFSKLQRENLSIIESYGTALMEVVCRDACDGHEIGRMLALAVLDRVLSIDRQCQWLVYLCNSGYLRVLVESLKQDDVSLQTLLTPQPPLLKPLYIYESKMALLTRVAKTAQGAMELLRCGLVAQLVECQVFHMLPQNDALRVFGQRDPSGFIPSPLERYRQILLPTLRLMQVILTSTTAQHQQGAAQVLQWLIVHSDVIQSILHGQDMSMGSLQELSLLTAIISKTALPGALEMGQEINSAALMELQGHIGRFQRQSLSLLVRLVGTDRARYLKQIEDTVSPSNLAEKREEMEVAMQQICANIMEYCQTLLLQSSSEAQFSLCLFSPSASEPADVSIPSARVPSLGLVLLLLKNSASDFFRYHDSHRQSLNKLERVEQLPPEELKELCQGLVSGSGGVEKISSVQRNVLAKRRLVQLVNNRAKLLALCSYIIETCLFVIWRHLEFYLLYCTPTDPKDSLLPGYRGQLSLKLQDYFQMLLNDMVSYQIALSADPAGSRGLSVSRVSQQDLEQLQSDMSSSFSEPLQRKLLEVEGLYSKSRSRHTFIQALTRRIRGLVHHAKS

Target ORF information:

RefSeq Version	XM_009293477.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio nucleoporin 205 (nup205), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293477.2

ORF Insert Sequence:

ATGGCGACGC AGATGGCGGT AAATTCAGGA GCCAGTCTGT GGGGGCCGCT GAAGGAGCTC 
TGGGAGGCTG TGGAAGGGGC CATTTGGAGA CGTCAACCAG AAAGTGTTCA TCTTTTAGAT 
CTGCAGTTGA AGAAACACAA ACCCTACTTC CTCAACCTCT TTAAAAACCC ACCGAAGAGT 
GCAGAACAGA GAGAGAAGGT TCGGAAAGCC AGTACAGAAG GCATTGCTAT CCAGGGTCAG 
CAGGGAGCAC GACTACTACC TGAGCAGCTC ATCTCTGAAG CCTTCATCCT TAGTGACCTT 
TTTGACCTTG GAGAACTAGC CGCCCTCGAG CTGCTGCTGG CAGGAGAGCA TCAGCAGCCT 
CATTTCCCCG GCCTGACCCG AGGCCTGGTA GCTGTGCTCC TTTACTGGGA TGGTAAACGC 
TGCGTGGCCA ACTCTCTCCG CTCACTTATC CAGTCCAGAC ATGGGAAGAC TTTCACTCTT 
GACTTGAGTC CTGAATTGGT GAATCTCACC ACACGGTTCA CAGATGAGCT GATGGCCCAG 
GGTTTGACCA AGCAAATACT GAACCTGGTC TCTGACGTCA GCGTGACCCG TGAGTTTGAG 
AGGCTGCAGA AAGAGCGTGG CCTTGGCAAT GAGAAACACA GGAAAGAGGT ATCTGACCTC 
ATTAAAGAGT GCCGACAGTC TCTGGCAGAG TGTTTGTTTG CGTGGGCATG CCAGTCACCG 
CTAGGCAAAG ATGACACGCT GGCTCTTATT GGGCATTTAG AGATGGTGAC CGCTGAAGCT 
GATGGCTCTT TGGATAGTGT CAACCTTGCT TTGGTCATGG CATTGCTCTA TTCCCTGGAT 
GTTAGTTTTC TGGAGCAAGG AACAGAAGAT AGAGAAGACC TGCTCCAGGC TTTGCCTCTG 
TTGACGGAGA AACAGTACGT GGCAGCCGTG CACAGTCGGC TGGTTGAGGG TCAAGGCTGG 
AAGCTTCCAG GACTGCAGGC AGTGGTGCAG CTGGCCTGGG CGCTGTCTCT GAGGGCCCTT 
TCACAACTTC CACAGGGTGC AGCTTTAGTG GAGTTCACAG AAGCAGATGA GGCTCTGGCG 
GACCAGGCAC TGTTGGGAGG CGTCTTCCTC TTTCTGACGG AGGGCGTCCT GGGAAGTGAT 
GGTTTCAGCC AAGAAGAATT TTACACTCGG CGGCTGCATT CTCTGATTAC TGATTTCCTG 
GCACTCATGC CAATGAAGGT GAAACAGTTG CGCAACCGTG CCGATGAAGA TGCTCGTCTG 
ATCCACATGG CTCTGCAGAT GGGCAGCGAG CCGCCATCAT CTCTTCGCAA GGACCTGGAC 
CACCTCATGA TCCTTATTGG AGAATTTTAC AGCAAAGATC CATTTGAACT TGAGCTGGCT 
TTAGAGTTTT GGTGTCCATC AGAGTCTCTT CAGCACACTT CACTCACTGG ATCTTTTCTT 
GGAGTACCTC TCCAAAGACC CCCACATAAA CAGGTGGTTC TCTCCAAGTT TGTGCGTCAG 
ATGGGTGATC TGCTTCCTGC GACTCTTTAC ATCCCATACC TGCGAATGCT GAAAGGACTT 
GCAAATGGCC CTCAGTGCTC CCACTACTGC TTCAGTCTGC TTAAAACTAA CGGCGCTCCA 
CACGGAGAGA ACCGGCAGGC TGGAGTGTCA GGCAGTCTTG TGTCATGGGA ACACTTTTTC 
CACTCGCTCA TGCTGTACCA TGAAAACCTG CGACGAGACG TGCCCAGTGC CGACAGCACA 
CAATACCGTC ATCTACCAAT CAGAGGCATC ACTCAGAGAG AGCTGGAAGG TCTCACTGCA 
TTCCTCCAGC TGCTCACCAC GATCATCACC TGGAGTGAGA ATGCACGTCT GGCTCTTTGT 
GAGCACCCTC AGTGGACCCC TGTGGTGGTG ATGCTGGGTT TGCTACAGTG TAGTGTTCAG 
CCCGTCCTCA AAGCTCAAGT CCTTCATGTT CTCGCTGCTT TTGGCAAATC TCCTGAGATT 
GCAGCCTCCC TCTGGCAGTC TCTAGAGTAC ACACAGATCC TACAAACAGT GAAAATCCCT 
GGTCAGAGAC AGGCTGCTGG GATCGAGGTG GAGCTGAATG AGATTGAATC GAGCTGTGAG 
GAGTATCCAC TCACACGGGC CTTTTGTCAT CTGATCAGTA CTCTGGTGGA GAGCGCACTT 
CCAGTCAATC TTGGAGCAGG ACTGCGAGCT CCCGGCTTCC AACCTTATCT TGACTTCCTG 
CGTGACTCTG TGTTTCTGGC CTTCCCCACG CGAGCCTACC GTCGCTCAGC AGAAAAGTGG 
GAGGTAGCTG AAGCAGTGCT AGAGGTTTTT CACAAACTTC TGCGAGAATA TGAGCCCCAG 
CCTTCAGACT TCCTGCCGGA GATGGTGGAA CTTCAAGGAG AGCAGGTGCC GGCCCATAAG 
CCCCCGGGGC ACAGCCTGAT GTTCCACCTT TTGAACGACT CACCCACCCT CTCTCTCTGC 
CTCAACCTAC TGGAGGAGGG CGCCCGCCAG CTTGACACAT ACGCTCAATT CCCTGGCAAG 
AAGCAGTTAG AGTCTGCAGT TCTGCACTGT CTGTGTTTGC TGGAGCTAGC CCTGCAGAAG 
GAGGTGACGT TCATGGATCT GCTGAGAGAA AGTCAAACAT CACTTCTTGT GTCTCCTTTG 
GAGCAGCTCC TGCAGGGCGT CAGTGCTCAG AGCAGAAAGG CAGATCACAT CACCAACATT 
GCTAGGTACC TGTATCACAG CAGCTCCAAT CCTGATGCTG CGTTCCAGAG TGCAAAGATC 
CTGCGACGGA TCACTCGCTA CCCAAACATC CAGGCCAGAC TGGTTGGAGA CTTCACTCAT 
GATCAGGCAG TGAGTGAGAG GTTGATGGCC GGCTTTGTGG AGTGTCTGGA CAGTGAAGAG 
GCTCAGGAAG GAGTGACCAC AAATGATTCA GACTCTGAGA AACGGGTGGC CAGAATTCGT 
CATGAGACCA AGATCCACAT CTTGAATCTT TTGATCACGT CCCTGGAGCT AACAGGCCCC 
AATCTGGGCC TGTATCTGCT TGGCTATGAA GTCAAGAAGC CAGTTTCCTC CACTAACCTT 
CAAGACCCAG GTGTTCTTGG CTGTCCGCGG AGCTGCCTGC ATGCGATTCT CAGTCTGCTT 
CAGAGGGGCA GTGACTCTCG CTCTGGACCT GGGCTCATTA AACAGGCTCC TCAACTAGCT 
GAACTCTGCT ACCAGGTGAT TTATCAGCTG TGCGCATGTC CTGACACCTC TGGCCCAACC 
ATGAGATACC TCAGGACCAG TCAGGACTTC CTGTTCTCTC ATCTGCAACA CCTACCTTTC 
GTTTTATCAG AAAATGAGAT CGCGGCTCTG TCTCAGATGT CCTGGCTAAT GAAAACCACA 
GCAATCGAGC TCCGAGTGAC CTCACTGAAC CGCCAGCGCT CACACACACA GAGACTCCTG 
AACCTCCTCC TCGATGACCA GCCACACACA TTACACACAG CAGATGGAGA GACCGGCATG 
GAGGAGGAGA ACAGATCAGT CAGTGGTTTT CTGCAGTTTG GCACCGTCTC CAAGGTGCGC 
AGGAGGCTGC TCAGTGTTTT GGATGCCATA GATTTCAGTC AGGAGGCACC AGAGCTTCTG 
CAGCTGGACT TCTTTGAGCG CACTCAGATC GAGCAGGTCA TCACTAACTG TGAACATGTC 
AACGAGCAGG GACACACCGT CTGCAATGTC AAGTTGCTGC ATCGAGTTTT AGTTGCAGAG 
ATCAATGCAC TACAGGGCAT GGCAGCCATC GGGCAAAGAC CACTGCTAAT GGAGGAGGTG 
AATTCAGTCC TGCAGCAGGT GGTGGAGAGG AATCGCGTGC GGCGCAGTCT CAGTGCCAAG 
CGTCATGCTC TGCAGAGCTG GAGGAGTCTG GTGGAGACCA TCCTCACCGC CTGTCCTTCA 
GAACTCATCC CTGCAGACCA GCGGCAGCTC ATCATCAGAG ATCTGCTGCT TGACCTGCAT 
GACAAGGTGT TATCAGAAGA TGCTGCTGGT GAACTGATGC CAATCGTAGC CGGAGCAGTC 
TTCACATTAA CAGCACACCT TAGCCAATCA GTGCTCTCTG AACAGCAGGG GGTGGGGCCA 
GAGGGTGGCT CTGGATCAGG ATTTGCATCC ATTGCTAACT CCGCCCTTCA CCTCATCCTG 
AGGAAGCTGC TGGACTTTAT CTTGTGCACA GGTGGAGGCT TTCAGCGTCT CAGGGCTCAT 
TTGTATGGAG CTCTGCTGTA CTACCTGCAG ATCGCCCAGA AACCTGAGGA GCCAGAGACA 
CTGCAGACAG GCACATCCAT GTGGGAGCGT TTGACTGCCC CTGAAGATTG CTTCTCCAAG 
CTACAGAGAG AAAACCTGTC CATCATCGAG AGCTATGGCA CTGCGCTAAT GGAAGTGGTG 
TGCAGAGACG CTTGTGATGG CCATGAGATC GGCCGAATGC TGGCTCTGGC CGTGTTAGAC 
AGGGTGTTGT CTATAGACAG GCAGTGTCAG TGGCTGGTGT ACTTGTGTAA CAGTGGTTAC 
CTGCGTGTGC TGGTGGAGAG TTTAAAGCAG GATGACGTTT CTTTGCAAAC TCTTCTCACG 
CCTCAGCCGC CGCTGCTTAA ACCGTTGTAC ATCTACGAGT CCAAAATGGC TCTGCTGACC 
CGCGTGGCCA AGACCGCTCA GGGTGCGATG GAGCTGCTGC GCTGTGGTCT GGTCGCTCAG 
CTGGTGGAGT GTCAGGTGTT TCACATGCTC CCTCAGAATG ACGCGCTCAG AGTGTTTGGG 
CAGAGAGATC CTTCAGGGTT TATTCCTAGT CCTCTGGAGC GATACAGGCA GATCCTGCTT 
CCTACACTCA GACTGATGCA GGTCATCTTG ACCTCCACCA CCGCACAGCA CCAGCAGGGG 
GCAGCACAGG TGCTCCAGTG GCTTATCGTT CATTCGGATG TCATTCAGTC TATTCTGCAT 
GGTCAGGACA TGAGCATGGG CTCTTTACAG GAACTGTCTC TGCTCACTGC CATCATCAGC 
AAGACGGCAC TGCCAGGAGC ACTTGAGATG GGACAGGAAA TCAACAGCGC TGCCCTCATG 
GAGTTACAGG GACACATCGG CAGATTCCAG CGGCAGTCGT TGTCTCTGCT GGTGCGTCTG 
GTGGGCACTG ATCGCGCTCG CTACCTTAAG CAGATTGAGG ACACCGTTTC TCCCAGCAAC 
CTAGCAGAAA AAAGAGAGGA AATGGAGGTT GCCATGCAAC AGATCTGTGC TAATATTATG 
GAGTACTGCC AGACGCTGCT GCTGCAGAGC TCATCTGAAG CCCAGTTCTC ACTCTGTCTG 
TTCAGCCCAT CAGCAAGTGA ACCAGCCGAT GTGTCCATCC CCTCTGCCCG CGTGCCCAGT 
CTGGGGCTGG TTCTGCTCCT GCTGAAAAAC AGCGCCAGCG ACTTCTTCAG GTATCACGAC 
AGTCACAGAC AGAGCCTGAA CAAGCTGGAG CGAGTGGAGC AGCTTCCTCC TGAGGAACTC 
AAAGAGCTGT GCCAGGGTTT GGTGTCTGGT TCTGGTGGAG TGGAAAAGAT CTCATCAGTG 
CAGAGAAACG TACTCGCCAA ACGGAGACTC GTCCAGCTGG TCAACAACAG AGCCAAGCTT 
CTTGCCCTCT GCTCTTATAT CATTGAGACG TGTCTGTTTG TCATCTGGCG CCACCTAGAG 
TTTTACTTGC TTTACTGCAC TCCCACTGAC CCCAAAGACT CACTGCTGCC AGGTTACAGA 
GGTCAGCTTT CTCTCAAGCT TCAGGATTAT TTTCAGATGC TGTTGAATGA CATGGTTTCT 
TACCAGATTG CTCTCTCTGC AGATCCTGCA GGCAGCAGAG GATTGAGCGT GTCGAGAGTG 
AGCCAGCAAG ATCTTGAACA GTTGCAGAGT GACATGTCGA GCAGCTTCAG TGAGCCGCTG 
CAGAGGAAGC TGCTGGAGGT GGAGGGACTT TATAGCAAAA GCCGCTCTCG TCACACCTTC 
ATCCAGGCCC TGACACGCAG GATCCGCGGC CTCGTACACC ACGCCAAAAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nup205 ( XM_021467634.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021323309.1 Danio rerio nucleoporin 205 (nup205), transcript variant X1, mRNA.

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CloneID	ODa149850
Clone ID Related Accession (Same CDS sequence)	XM_021467634.1
Accession Version	XM_021467634.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6042bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	nuclear pore complex protein Nup205 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTCAC ACCGGCAGAA TTCAGGGCAG CCCACTCACG CTTCAGACAT GGCGACGCAG 
ATGGCGGTAA ATTCAGGAGC CAGTCTGTGG GGGCCGCTGA AGGAGCTCTG GGAGGCTGTG 
GAAGGGGCCA TTTGGAGACG TCAACCAGAA AGTGTTCATC TTTTAGATCT GCAGTTGAAG 
AAACACAAAC CCTACTTCCT CAACCTCTTT AAAAACCCAC CGAAGAGTGC AGAACAGAGA 
GAGAAGGTTC GGAAAGCCAG TACAGAAGGC ATTGCTATCC AGGGTCAGCA GGGAGCACGA 
CTACTACCTG AGCAGCTCAT CTCTGAAGCC TTCATCCTTA GTGACCTTTT TGACCTTGGA 
GAACTAGCCG CCCTCGAGCT GCTGCTGGCA GGAGAGCATC AGCAGCCTCA TTTCCCCGGC 
CTGACCCGAG GCCTGGTAGC TGTGCTCCTT TACTGGGATG GTAAACGCTG CGTGGCCAAC 
TCTCTCCGCT CACTTATCCA GTCCAGACAT GGGAAGACTT TCACTCTTGA CTTGAGTCCT 
GAATTGGTGA ATCTCACCAC ACGGTTCACA GATGAGCTGA TGGCCCAGGG TTTGACCAAG 
CAAATACTGA ACCTGGTCTC TGACGTCAGC GTGACCCGTG AGTTTGAGAG GCTGCAGAAA 
GAGCGTGGCC TTGGCAATGA GAAACACAGG AAAGAGGTAT CTGACCTCAT TAAAGAGTGC 
CGACAGTCTC TGGCAGAGTG TTTGTTTGCG TGGGCATGCC AGTCACCGCT AGGCAAAGAT 
GACACGCTGG CTCTTATTGG GCATTTAGAG ATGGTGACCG CTGAAGCTGA TGGCTCTTTG 
GATAGTGTCA ACCTTGCTTT GGTCATGGCA TTGCTCTATT CCCTGGATGT TAGTTTTCTG 
GAGCAAGGAA CAGAAGATAG AGAAGACCTG CTCCAGGCTT TGCCTCTGTT GACGGAGAAA 
CAGTACGTGG CAGCCGTGCA CAGTCGGCTG GTTGAGGGTC AAGGCTGGAA GCTTCCAGGA 
CTGCAGGCAG TGGTGCAGCT GGCCTGGGCG CTGTCTCTGA GGGCCCTTTC ACAACTTCCA 
CAGGGTGCAG CTTTAGTGGA GTTCACAGAA GCAGATGAGG CTCTGGCGGA CCAGGCACTG 
TTGGGAGGCG TCTTCCTCTT TCTGACGGAG GGCGTCCTGG GAAGTGATGG TTTCAGCCAA 
GAAGAATTTT ACACTCGGCG GCTGCATTCT CTGATTACTG ATTTCCTGGC ACTCATGCCA 
ATGAAGGTGA AACAGTTGCG CAACCGTGCC GATGAAGATG CTCGTCTGAT CCACATGGCT 
CTGCAGATGG GCAGCGAGCC GCCATCATCT CTTCGCAAGG ACCTGGACCA CCTCATGATC 
CTTATTGGAG AATTTTACAG CAAAGATCCA TTTGAACTTG AGCTGGCTTT AGAGTTTTGG 
TGTCCATCAG AGTCTCTTCA GCACACTTCA CTCACTGGAT CTTTTCTTGG AGTACCTCTC 
CAAAGACCCC CACATAAACA GGTGGTTCTC TCCAAGTTTG TGCGTCAGAT GGGTGATCTG 
CTTCCTGCGA CTCTTTACAT CCCATACCTG CGAATGCTGA AAGGACTTGC AAATGGCCCT 
CAGTGCTCCC ACTACTGCTT CAGTCTGCTT AAAACTAACG GCGCTCCACA CGGAGAGAAC 
CGGCAGGCTG GAGTGTCAGG CAGTCTTGTG TCATGGGAAC ACTTTTTCCA CTCGCTCATG 
CTGTACCATG AAAACCTGCG ACGAGACGTG CCCAGTGCCG ACAGCACACA ATACCGTCAT 
CTACCAATCA GAGGCATCAC TCAGAGAGAG CTGGAAGGTC TCACTGCATT CCTCCAGCTG 
CTCACCACGA TCATCACCTG GAGTGAGAAT GCACGTCTGG CTCTTTGTGA GCACCCTCAG 
TGGACCCCTG TGGTGGTGAT GCTGGGTTTG CTACAGTGTA GTGTTCAGCC CGTCCTCAAA 
GCTCAAGTCC TTCATGTTCT CGCTGCTTTT GGCAAATCTC CTGAGATTGC AGCCTCCCTC 
TGGCAGTCTC TAGAGTACAC ACAGATCCTA CAAACAGTGA AAATCCCTGG TCAGAGACAG 
GCTGCTGGGA TCGAGGTGGA GCTGAATGAG ATTGAATCGA GCTGTGAGGA GTATCCACTC 
ACACGGGCCT TTTGTCATCT GATCAGTACT CTGGTGGAGA GCGCACTTCC AGTCAATCTT 
GGAGCAGGAC TGCGAGCTCC CGGCTTCCAA CCTTATCTTG ACTTCCTGCG TGACTCTGTG 
TTTCTGGCCT TCCCCACGCG AGCCTACCGT CGCTCAGCAG AAAAGTGGGA GGTAGCTGAA 
GCAGTGCTAG AGGTTTTTCA CAAACTTCTG CGAGAATATG AGCCCCAGCC TTCAGACTTC 
CTGCCGGAGA TGGTGGAACT TCAAGGAGAG CAGGTGCCGG CCCATAAGCC CCCGGGGCAC 
AGCCTGATGT TCCACCTTTT GAACGACTCA CCCACCCTCT CTCTCTGCCT CAACCTACTG 
GAGGAGGGCG CCCGCCAGCT TGACACATAC GCTCAATTCC CTGGCAAGAA GCAGTTAGAG 
TCTGCAGTTC TGCACTGTCT GTGTTTGCTG GAGCTAGCCC TGCAGAAGGA GGTGACGTTC 
ATGGATCTGC TGAGAGAAAG TCAAACATCA CTTCTTGTGT CTCCTTTGGA GCAGCTCCTG 
CAGGGCGTCA GTGCTCAGAG CAGAAAGGCA GATCACATCA CCAACATTGC TAGGTACCTG 
TATCACAGCA GCTCCAATCC TGATGCTGCG TTCCAGAGTG CAAAGATCCT GCGACGGATC 
ACTCGCTACC CAAACATCCA GGCCAGACTG GTTGGAGACT TCACTCATGA TCAGGCAGTG 
AGTGAGAGGT TGATGGCCGG CTTTGTGGAG TGTCTGGACA GTGAAGAGGC TCAGGAAGGA 
GTGACCACAA ATGATTCAGA CTCTGAGAAA CGGGTGGCCA GAATTCGTCA TGAGACCAAG 
ATCCACATCT TGAATCTTTT GATCACGTCC CTGGAGCTAA CAGGCCCCAA TCTGGGCCTG 
TATCTGCTTG GCTATGAAGT CAAGAAGCCA GTTTCCTCCA CTAACCTTCA AGACCCAGGT 
GTTCTTGGCT GTCCGCGGAG CTGCCTGCAT GCGATTCTCA GTCTGCTTCA GAGGGGCAGT 
GACTCTCGCT CTGGACCTGG GCTCATTAAA CAGGCTCCTC AACTAGCTGA ACTCTGCTAC 
CAGGTGATTT ATCAGCTGTG CGCATGTCCT GACACCTCTG GCCCAACCAT GAGATACCTC 
AGGACCAGTC AGGACTTCCT GTTCTCTCAT CTGCAACACC TACCTTTCGT TTTATCAGAA 
AATGAGATCG CGGCTCTGTC TCAGATGTCC TGGCTAATGA AAACCACAGC AATCGAGCTC 
CGAGTGACCT CACTGAACCG CCAGCGCTCA CACACACAGA GACTCCTGAA CCTCCTCCTC 
GATGACCAGC CACACACATT ACACACAGCA GATGGAGAGA CCGGCATGGA GGAGGAGAAC 
AGATCAGTCA GTGGTTTTCT GCAGTTTGGC ACCGTCTCCA AGGTGCGCAG GAGGCTGCTC 
AGTGTTTTGG ATGCCATAGA TTTCAGTCAG GAGGCACCAG AGCTTCTGCA GCTGGACTTC 
TTTGAGCGCA CTCAGATCGA GCAGGTCATC ACTAACTGTG AACATGTCAA CGAGCAGGGA 
CACACCGTCT GCAATGTCAA GTTGCTGCAT CGAGTTTTAG TTGCAGAGAT CAATGCACTA 
CAGGGCATGG CAGCCATCGG GCAAAGACCA CTGCTAATGG AGGAGGTGAA TTCAGTCCTG 
CAGCAGGTGG TGGAGAGGAA TCGCGTGCGG CGCAGTCTCA GTGCCAAGCG TCATGCTCTG 
CAGAGCTGGA GGAGTCTGGT GGAGACCATC CTCACCGCCT GTCCTTCAGA ACTCATCCCT 
GCAGACCAGC GGCAGCTCAT CATCAGAGAT CTGCTGCTTG ACCTGCATGA CAAGGTGTTA 
TCAGAAGATG CTGCTGGTGA ACTGATGCCA ATCGTAGCCG GAGCAGTCTT CACATTAACA 
GCACACCTTA GCCAATCAGT GCTCTCTGAA CAGCAGGGGG TGGGGCCAGA GGGTGGCTCT 
GGATCAGGAT TTGCATCCAT TGCTAACTCC GCCCTTCACC TCATCCTGAG GAAGCTGCTG 
GACTTTATCT TGTGCACAGG TGGAGGCTTT CAGCGTCTCA GGGCTCATTT GTATGGAGCT 
CTGCTGTACT ACCTGCAGAT CGCCCAGAAA CCTGAGGAGC CAGAGACACT GCAGACAGGC 
ACATCCATGT GGGAGCGTTT GACTGCCCCT GAAGATTGCT TCTCCAAGCT ACAGAGAGAA 
AACCTGTCCA TCATCGAGAG CTATGGCACT GCGCTAATGG AAGTGGTGTG CAGAGACGCT 
TGTGATGGCC ATGAGATCGG CCGAATGCTG GCTCTGGCCG TGTTAGACAG GGTGTTGTCT 
ATAGACAGGC AGTGTCAGTG GCTGGTGTAC TTGTGTAACA GTGGTTACCT GCGTGTGCTG 
GTGGAGAGTT TAAAGCAGGA TGACGTTTCT TTGCAAACTC TTCTCACGCC TCAGCCGCCG 
CTGCTTAAAC CGTTGTACAT CTACGAGTCC AAAATGGCTC TGCTGACCCG CGTGGCCAAG 
ACCGCTCAGG GTGCGATGGA GCTGCTGCGC TGTGGTCTGG TCGCTCAGCT GGTGGAGTGT 
CAGGTGTTTC ACATGCTCCC TCAGAATGAC GCGCTCAGAG TGTTTGGGCA GAGAGATCCT 
TCAGGGTTTA TTCCTAGTCC TCTGGAGCGA TACAGGCAGA TCCTGCTTCC TACACTCAGA 
CTGATGCAGG TCATCTTGAC CTCCACCACC GCACAGCACC AGCAGGGGGC AGCACAGGTG 
CTCCAGTGGC TTATCGTTCA TTCGGATGTC ATTCAGTCTA TTCTGCATGG TCAGGACATG 
AGCATGGGCT CTTTACAGGA ACTGTCTCTG CTCACTGCCA TCATCAGCAA GACGGCACTG 
CCAGGAGCAC TTGAGATGGG ACAGGAAATC AACAGCGCTG CCCTCATGGA GTTACAGGGA 
CACATCGGCA GATTCCAGCG GCAGTCGTTG TCTCTGCTGG TGCGTCTGGT GGGCACTGAT 
CGCGCTCGCT ACCTTAAGCA GATTGAGGAC ACCGTTTCTC CCAGCAACCT AGCAGAAAAA 
AGAGAGGAAA TGGAGGTTGC CATGCAACAG ATCTGTGCTA ATATTATGGA GTACTGCCAG 
ACGCTGCTGC TGCAGAGCTC ATCTGAAGCC CAGTTCTCAC TCTGTCTGTT CAGCCCATCA 
GCAAGTGAAC CAGCCGATGT GTCCATCCCC TCTGCCCGCG TGCCCAGTCT GGGGCTGGTT 
CTGCTCCTGC TGAAAAACAG CGCCAGCGAC TTCTTCAGGT ATCACGACAG TCACAGACAG 
AGCCTGAACA AGCTGGAGCG AGTGGAGCAG CTTCCTCCTG AGGAACTCAA AGAGCTGTGC 
CAGGGTTTGG TGTCTGGTTC TGGTGGAGTG GAAAAGATCT CATCAGTGCA GAGAAACGTA 
CTCGCCAAAC GGAGACTCGT CCAGCTGGTC AACAACAGAG CCAAGCTTCT TGCCCTCTGC 
TCTTATATCA TTGAGACGTG TCTGTTTGTC ATCTGGCGCC ACCTAGAGTT TTACTTGCTT 
TACTGCACTC CCACTGACCC CAAAGACTCA CTGCTGCCAG GTTACAGAGG TCAGCTTTCT 
CTCAAGCTTC AGGATTATTT TCAGATGCTG TTGAATGACA TGGTTTCTTA CCAGATTGCT 
CTCTCTGCAG ATCCTGCAGG CAGCAGAGGA TTGAGCGTGT CGAGAGTGAG CCAGCAAGAT 
CTTGAACAGT TGCAGAGTGA CATGTCGAGC AGCTTCAGTG AGCCGCTGCA GAGGAAGCTG 
CTGGAGGTGG AGGGACTTTA TAGCAAAAGC CGCTCTCGTC ACACCTTCAT CCAGGCCCTG 
ACACGCAGGA TCCGCGGCCT CGTACACCAC GCCAAAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021323309.1
CDS	32..6073
Translation	MFSHRQNSGQPTHASDMATQMAVNSGASLWGPLKELWEAVEGAIWRRQPESVHLLDLQLKKHKPYFLNLFKNPPKSAEQREKVRKASTEGIAIQGQQGARLLPEQLISEAFILSDLFDLGELAALELLLAGEHQQPHFPGLTRGLVAVLLYWDGKRCVANSLRSLIQSRHGKTFTLDLSPELVNLTTRFTDELMAQGLTKQILNLVSDVSVTREFERLQKERGLGNEKHRKEVSDLIKECRQSLAECLFAWACQSPLGKDDTLALIGHLEMVTAEADGSLDSVNLALVMALLYSLDVSFLEQGTEDREDLLQALPLLTEKQYVAAVHSRLVEGQGWKLPGLQAVVQLAWALSLRALSQLPQGAALVEFTEADEALADQALLGGVFLFLTEGVLGSDGFSQEEFYTRRLHSLITDFLALMPMKVKQLRNRADEDARLIHMALQMGSEPPSSLRKDLDHLMILIGEFYSKDPFELELALEFWCPSESLQHTSLTGSFLGVPLQRPPHKQVVLSKFVRQMGDLLPATLYIPYLRMLKGLANGPQCSHYCFSLLKTNGAPHGENRQAGVSGSLVSWEHFFHSLMLYHENLRRDVPSADSTQYRHLPIRGITQRELEGLTAFLQLLTTIITWSENARLALCEHPQWTPVVVMLGLLQCSVQPVLKAQVLHVLAAFGKSPEIAASLWQSLEYTQILQTVKIPGQRQAAGIEVELNEIESSCEEYPLTRAFCHLISTLVESALPVNLGAGLRAPGFQPYLDFLRDSVFLAFPTRAYRRSAEKWEVAEAVLEVFHKLLREYEPQPSDFLPEMVELQGEQVPAHKPPGHSLMFHLLNDSPTLSLCLNLLEEGARQLDTYAQFPGKKQLESAVLHCLCLLELALQKEVTFMDLLRESQTSLLVSPLEQLLQGVSAQSRKADHITNIARYLYHSSSNPDAAFQSAKILRRITRYPNIQARLVGDFTHDQAVSERLMAGFVECLDSEEAQEGVTTNDSDSEKRVARIRHETKIHILNLLITSLELTGPNLGLYLLGYEVKKPVSSTNLQDPGVLGCPRSCLHAILSLLQRGSDSRSGPGLIKQAPQLAELCYQVIYQLCACPDTSGPTMRYLRTSQDFLFSHLQHLPFVLSENEIAALSQMSWLMKTTAIELRVTSLNRQRSHTQRLLNLLLDDQPHTLHTADGETGMEEENRSVSGFLQFGTVSKVRRRLLSVLDAIDFSQEAPELLQLDFFERTQIEQVITNCEHVNEQGHTVCNVKLLHRVLVAEINALQGMAAIGQRPLLMEEVNSVLQQVVERNRVRRSLSAKRHALQSWRSLVETILTACPSELIPADQRQLIIRDLLLDLHDKVLSEDAAGELMPIVAGAVFTLTAHLSQSVLSEQQGVGPEGGSGSGFASIANSALHLILRKLLDFILCTGGGFQRLRAHLYGALLYYLQIAQKPEEPETLQTGTSMWERLTAPEDCFSKLQRENLSIIESYGTALMEVVCRDACDGHEIGRMLALAVLDRVLSIDRQCQWLVYLCNSGYLRVLVESLKQDDVSLQTLLTPQPPLLKPLYIYESKMALLTRVAKTAQGAMELLRCGLVAQLVECQVFHMLPQNDALRVFGQRDPSGFIPSPLERYRQILLPTLRLMQVILTSTTAQHQQGAAQVLQWLIVHSDVIQSILHGQDMSMGSLQELSLLTAIISKTALPGALEMGQEINSAALMELQGHIGRFQRQSLSLLVRLVGTDRARYLKQIEDTVSPSNLAEKREEMEVAMQQICANIMEYCQTLLLQSSSEAQFSLCLFSPSASEPADVSIPSARVPSLGLVLLLLKNSASDFFRYHDSHRQSLNKLERVEQLPPEELKELCQGLVSGSGGVEKISSVQRNVLAKRRLVQLVNNRAKLLALCSYIIETCLFVIWRHLEFYLLYCTPTDPKDSLLPGYRGQLSLKLQDYFQMLLNDMVSYQIALSADPAGSRGLSVSRVSQQDLEQLQSDMSSSFSEPLQRKLLEVEGLYSKSRSRHTFIQALTRRIRGLVHHAKS

Target ORF information:

RefSeq Version	XM_021467634.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nucleoporin 205 (nup205), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467634.1

ORF Insert Sequence:

ATGTTTTCAC ACCGGCAGAA TTCAGGGCAG CCCACTCACG CTTCAGACAT GGCGACGCAG 
ATGGCGGTAA ATTCAGGAGC CAGTCTGTGG GGGCCGCTGA AGGAGCTCTG GGAGGCTGTG 
GAAGGGGCCA TTTGGAGACG TCAACCAGAA AGTGTTCATC TTTTAGATCT GCAGTTGAAG 
AAACACAAAC CCTACTTCCT CAACCTCTTT AAAAACCCAC CGAAGAGTGC AGAACAGAGA 
GAGAAGGTTC GGAAAGCCAG TACAGAAGGC ATTGCTATCC AGGGTCAGCA GGGAGCACGA 
CTACTACCTG AGCAGCTCAT CTCTGAAGCC TTCATCCTTA GTGACCTTTT TGACCTTGGA 
GAACTAGCCG CCCTCGAGCT GCTGCTGGCA GGAGAGCATC AGCAGCCTCA TTTCCCCGGC 
CTGACCCGAG GCCTGGTAGC TGTGCTCCTT TACTGGGATG GTAAACGCTG CGTGGCCAAC 
TCTCTCCGCT CACTTATCCA GTCCAGACAT GGGAAGACTT TCACTCTTGA CTTGAGTCCT 
GAATTGGTGA ATCTCACCAC ACGGTTCACA GATGAGCTGA TGGCCCAGGG TTTGACCAAG 
CAAATACTGA ACCTGGTCTC TGACGTCAGC GTGACCCGTG AGTTTGAGAG GCTGCAGAAA 
GAGCGTGGCC TTGGCAATGA GAAACACAGG AAAGAGGTAT CTGACCTCAT TAAAGAGTGC 
CGACAGTCTC TGGCAGAGTG TTTGTTTGCG TGGGCATGCC AGTCACCGCT AGGCAAAGAT 
GACACGCTGG CTCTTATTGG GCATTTAGAG ATGGTGACCG CTGAAGCTGA TGGCTCTTTG 
GATAGTGTCA ACCTTGCTTT GGTCATGGCA TTGCTCTATT CCCTGGATGT TAGTTTTCTG 
GAGCAAGGAA CAGAAGATAG AGAAGACCTG CTCCAGGCTT TGCCTCTGTT GACGGAGAAA 
CAGTACGTGG CAGCCGTGCA CAGTCGGCTG GTTGAGGGTC AAGGCTGGAA GCTTCCAGGA 
CTGCAGGCAG TGGTGCAGCT GGCCTGGGCG CTGTCTCTGA GGGCCCTTTC ACAACTTCCA 
CAGGGTGCAG CTTTAGTGGA GTTCACAGAA GCAGATGAGG CTCTGGCGGA CCAGGCACTG 
TTGGGAGGCG TCTTCCTCTT TCTGACGGAG GGCGTCCTGG GAAGTGATGG TTTCAGCCAA 
GAAGAATTTT ACACTCGGCG GCTGCATTCT CTGATTACTG ATTTCCTGGC ACTCATGCCA 
ATGAAGGTGA AACAGTTGCG CAACCGTGCC GATGAAGATG CTCGTCTGAT CCACATGGCT 
CTGCAGATGG GCAGCGAGCC GCCATCATCT CTTCGCAAGG ACCTGGACCA CCTCATGATC 
CTTATTGGAG AATTTTACAG CAAAGATCCA TTTGAACTTG AGCTGGCTTT AGAGTTTTGG 
TGTCCATCAG AGTCTCTTCA GCACACTTCA CTCACTGGAT CTTTTCTTGG AGTACCTCTC 
CAAAGACCCC CACATAAACA GGTGGTTCTC TCCAAGTTTG TGCGTCAGAT GGGTGATCTG 
CTTCCTGCGA CTCTTTACAT CCCATACCTG CGAATGCTGA AAGGACTTGC AAATGGCCCT 
CAGTGCTCCC ACTACTGCTT CAGTCTGCTT AAAACTAACG GCGCTCCACA CGGAGAGAAC 
CGGCAGGCTG GAGTGTCAGG CAGTCTTGTG TCATGGGAAC ACTTTTTCCA CTCGCTCATG 
CTGTACCATG AAAACCTGCG ACGAGACGTG CCCAGTGCCG ACAGCACACA ATACCGTCAT 
CTACCAATCA GAGGCATCAC TCAGAGAGAG CTGGAAGGTC TCACTGCATT CCTCCAGCTG 
CTCACCACGA TCATCACCTG GAGTGAGAAT GCACGTCTGG CTCTTTGTGA GCACCCTCAG 
TGGACCCCTG TGGTGGTGAT GCTGGGTTTG CTACAGTGTA GTGTTCAGCC CGTCCTCAAA 
GCTCAAGTCC TTCATGTTCT CGCTGCTTTT GGCAAATCTC CTGAGATTGC AGCCTCCCTC 
TGGCAGTCTC TAGAGTACAC ACAGATCCTA CAAACAGTGA AAATCCCTGG TCAGAGACAG 
GCTGCTGGGA TCGAGGTGGA GCTGAATGAG ATTGAATCGA GCTGTGAGGA GTATCCACTC 
ACACGGGCCT TTTGTCATCT GATCAGTACT CTGGTGGAGA GCGCACTTCC AGTCAATCTT 
GGAGCAGGAC TGCGAGCTCC CGGCTTCCAA CCTTATCTTG ACTTCCTGCG TGACTCTGTG 
TTTCTGGCCT TCCCCACGCG AGCCTACCGT CGCTCAGCAG AAAAGTGGGA GGTAGCTGAA 
GCAGTGCTAG AGGTTTTTCA CAAACTTCTG CGAGAATATG AGCCCCAGCC TTCAGACTTC 
CTGCCGGAGA TGGTGGAACT TCAAGGAGAG CAGGTGCCGG CCCATAAGCC CCCGGGGCAC 
AGCCTGATGT TCCACCTTTT GAACGACTCA CCCACCCTCT CTCTCTGCCT CAACCTACTG 
GAGGAGGGCG CCCGCCAGCT TGACACATAC GCTCAATTCC CTGGCAAGAA GCAGTTAGAG 
TCTGCAGTTC TGCACTGTCT GTGTTTGCTG GAGCTAGCCC TGCAGAAGGA GGTGACGTTC 
ATGGATCTGC TGAGAGAAAG TCAAACATCA CTTCTTGTGT CTCCTTTGGA GCAGCTCCTG 
CAGGGCGTCA GTGCTCAGAG CAGAAAGGCA GATCACATCA CCAACATTGC TAGGTACCTG 
TATCACAGCA GCTCCAATCC TGATGCTGCG TTCCAGAGTG CAAAGATCCT GCGACGGATC 
ACTCGCTACC CAAACATCCA GGCCAGACTG GTTGGAGACT TCACTCATGA TCAGGCAGTG 
AGTGAGAGGT TGATGGCCGG CTTTGTGGAG TGTCTGGACA GTGAAGAGGC TCAGGAAGGA 
GTGACCACAA ATGATTCAGA CTCTGAGAAA CGGGTGGCCA GAATTCGTCA TGAGACCAAG 
ATCCACATCT TGAATCTTTT GATCACGTCC CTGGAGCTAA CAGGCCCCAA TCTGGGCCTG 
TATCTGCTTG GCTATGAAGT CAAGAAGCCA GTTTCCTCCA CTAACCTTCA AGACCCAGGT 
GTTCTTGGCT GTCCGCGGAG CTGCCTGCAT GCGATTCTCA GTCTGCTTCA GAGGGGCAGT 
GACTCTCGCT CTGGACCTGG GCTCATTAAA CAGGCTCCTC AACTAGCTGA ACTCTGCTAC 
CAGGTGATTT ATCAGCTGTG CGCATGTCCT GACACCTCTG GCCCAACCAT GAGATACCTC 
AGGACCAGTC AGGACTTCCT GTTCTCTCAT CTGCAACACC TACCTTTCGT TTTATCAGAA 
AATGAGATCG CGGCTCTGTC TCAGATGTCC TGGCTAATGA AAACCACAGC AATCGAGCTC 
CGAGTGACCT CACTGAACCG CCAGCGCTCA CACACACAGA GACTCCTGAA CCTCCTCCTC 
GATGACCAGC CACACACATT ACACACAGCA GATGGAGAGA CCGGCATGGA GGAGGAGAAC 
AGATCAGTCA GTGGTTTTCT GCAGTTTGGC ACCGTCTCCA AGGTGCGCAG GAGGCTGCTC 
AGTGTTTTGG ATGCCATAGA TTTCAGTCAG GAGGCACCAG AGCTTCTGCA GCTGGACTTC 
TTTGAGCGCA CTCAGATCGA GCAGGTCATC ACTAACTGTG AACATGTCAA CGAGCAGGGA 
CACACCGTCT GCAATGTCAA GTTGCTGCAT CGAGTTTTAG TTGCAGAGAT CAATGCACTA 
CAGGGCATGG CAGCCATCGG GCAAAGACCA CTGCTAATGG AGGAGGTGAA TTCAGTCCTG 
CAGCAGGTGG TGGAGAGGAA TCGCGTGCGG CGCAGTCTCA GTGCCAAGCG TCATGCTCTG 
CAGAGCTGGA GGAGTCTGGT GGAGACCATC CTCACCGCCT GTCCTTCAGA ACTCATCCCT 
GCAGACCAGC GGCAGCTCAT CATCAGAGAT CTGCTGCTTG ACCTGCATGA CAAGGTGTTA 
TCAGAAGATG CTGCTGGTGA ACTGATGCCA ATCGTAGCCG GAGCAGTCTT CACATTAACA 
GCACACCTTA GCCAATCAGT GCTCTCTGAA CAGCAGGGGG TGGGGCCAGA GGGTGGCTCT 
GGATCAGGAT TTGCATCCAT TGCTAACTCC GCCCTTCACC TCATCCTGAG GAAGCTGCTG 
GACTTTATCT TGTGCACAGG TGGAGGCTTT CAGCGTCTCA GGGCTCATTT GTATGGAGCT 
CTGCTGTACT ACCTGCAGAT CGCCCAGAAA CCTGAGGAGC CAGAGACACT GCAGACAGGC 
ACATCCATGT GGGAGCGTTT GACTGCCCCT GAAGATTGCT TCTCCAAGCT ACAGAGAGAA 
AACCTGTCCA TCATCGAGAG CTATGGCACT GCGCTAATGG AAGTGGTGTG CAGAGACGCT 
TGTGATGGCC ATGAGATCGG CCGAATGCTG GCTCTGGCCG TGTTAGACAG GGTGTTGTCT 
ATAGACAGGC AGTGTCAGTG GCTGGTGTAC TTGTGTAACA GTGGTTACCT GCGTGTGCTG 
GTGGAGAGTT TAAAGCAGGA TGACGTTTCT TTGCAAACTC TTCTCACGCC TCAGCCGCCG 
CTGCTTAAAC CGTTGTACAT CTACGAGTCC AAAATGGCTC TGCTGACCCG CGTGGCCAAG 
ACCGCTCAGG GTGCGATGGA GCTGCTGCGC TGTGGTCTGG TCGCTCAGCT GGTGGAGTGT 
CAGGTGTTTC ACATGCTCCC TCAGAATGAC GCGCTCAGAG TGTTTGGGCA GAGAGATCCT 
TCAGGGTTTA TTCCTAGTCC TCTGGAGCGA TACAGGCAGA TCCTGCTTCC TACACTCAGA 
CTGATGCAGG TCATCTTGAC CTCCACCACC GCACAGCACC AGCAGGGGGC AGCACAGGTG 
CTCCAGTGGC TTATCGTTCA TTCGGATGTC ATTCAGTCTA TTCTGCATGG TCAGGACATG 
AGCATGGGCT CTTTACAGGA ACTGTCTCTG CTCACTGCCA TCATCAGCAA GACGGCACTG 
CCAGGAGCAC TTGAGATGGG ACAGGAAATC AACAGCGCTG CCCTCATGGA GTTACAGGGA 
CACATCGGCA GATTCCAGCG GCAGTCGTTG TCTCTGCTGG TGCGTCTGGT GGGCACTGAT 
CGCGCTCGCT ACCTTAAGCA GATTGAGGAC ACCGTTTCTC CCAGCAACCT AGCAGAAAAA 
AGAGAGGAAA TGGAGGTTGC CATGCAACAG ATCTGTGCTA ATATTATGGA GTACTGCCAG 
ACGCTGCTGC TGCAGAGCTC ATCTGAAGCC CAGTTCTCAC TCTGTCTGTT CAGCCCATCA 
GCAAGTGAAC CAGCCGATGT GTCCATCCCC TCTGCCCGCG TGCCCAGTCT GGGGCTGGTT 
CTGCTCCTGC TGAAAAACAG CGCCAGCGAC TTCTTCAGGT ATCACGACAG TCACAGACAG 
AGCCTGAACA AGCTGGAGCG AGTGGAGCAG CTTCCTCCTG AGGAACTCAA AGAGCTGTGC 
CAGGGTTTGG TGTCTGGTTC TGGTGGAGTG GAAAAGATCT CATCAGTGCA GAGAAACGTA 
CTCGCCAAAC GGAGACTCGT CCAGCTGGTC AACAACAGAG CCAAGCTTCT TGCCCTCTGC 
TCTTATATCA TTGAGACGTG TCTGTTTGTC ATCTGGCGCC ACCTAGAGTT TTACTTGCTT 
TACTGCACTC CCACTGACCC CAAAGACTCA CTGCTGCCAG GTTACAGAGG TCAGCTTTCT 
CTCAAGCTTC AGGATTATTT TCAGATGCTG TTGAATGACA TGGTTTCTTA CCAGATTGCT 
CTCTCTGCAG ATCCTGCAGG CAGCAGAGGA TTGAGCGTGT CGAGAGTGAG CCAGCAAGAT 
CTTGAACAGT TGCAGAGTGA CATGTCGAGC AGCTTCAGTG AGCCGCTGCA GAGGAAGCTG 
CTGGAGGTGG AGGGACTTTA TAGCAAAAGC CGCTCTCGTC ACACCTTCAT CCAGGCCCTG 
ACACGCAGGA TCCGCGGCCT CGTACACCAC GCCAAAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of 315 genes essential for early zebrafish development.
Proceedings of the National Academy of Sciences of the United States of America101(35)12792-7(2004 Aug)
Amsterdam A,Nissen RM,Sun Z,Swindell EC,Farrington S,Hopkins N

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nup205 cDNA ORF clone, Danio rerio(zebrafish)

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