egfl6 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	egfl6
Entrez Gene ID	436730
Full Name	EGF-like-domain, multiple 6
Synonyms	sb:cb3,wu:fc66b04,zgc:92328
General protein information	Preferred Names EGF-like-domain, multiple 6 Names epidermal growth factor-like protein 6 cb3
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017357665.1	XP_017213154.1	epidermal growth factor-like protein 6 isoform X2
NM_001002457.1	NP_001002457.1	epidermal growth factor-like protein 6
XM_021478639.1	XP_021334314.1	epidermal growth factor-like protein 6 isoform X2
XM_017357664.1	XP_017213153.1	epidermal growth factor-like protein 6 isoform X1
XM_017357664.2	XP_017213153.1	epidermal growth factor-like protein 6 isoform X1

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	EGFL6	XP_002806205.1
Mus musculus (house mouse)	Egfl6	NP_062270.1
Gallus gallus (chicken)	EGFL6	NP_001264667.1
Xenopus tropicalis (tropical clawed frog)	egfl6	XP_002933858.2
Canis lupus familiaris (dog)	EGFL6	XP_548865.2
Homo sapiens (human)	EGFL6	NP_001161362.1
Rattus norvegicus (Norway rat)	Egfl6	NP_001101724.1
Pan troglodytes (chimpanzee)	EGFL6	XP_528894.1
Bos taurus (cattle)	EGFL6	XP_002700453.2
Danio rerio (zebrafish)	egfl6	NP_001002457.1

Gene Ontology
Bibliography

Related articles in PubMed

Analysis of novel caudal hindbrain genes reveals different regulatory logic for gene expression in rhombomere 4 versus 5/6 in embryonic zebrafish.
Ghosh P, Maurer JM, Sagerström CG
Neural development13(1)13(2018 06)

The somite-secreted factor Maeg promotes zebrafish embryonic angiogenesis.
Wang X, Yuan W, Wang X, Qi J, Qin Y, Shi Y, Zhang J, Gong J, Dong Z, Liu X, Sun C, Chai R, Le Noble F, Liu D
Oncotarget7(47)77749-77763(2016 Nov)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Egfl6 is involved in zebrafish notochord development.
Wang X, Wang X, Yuan W, Chai R, Liu D
Fish physiology and biochemistry41(4)961-9(2015 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following egfl6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the egfl6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa03897	NM_001002457.1 Latest version!	Danio rerio EGF-like-domain, multiple 6 (egfl6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODa03897	XM_021478639.1 Latest version!	Danio rerio EGF-like-domain, multiple 6 (egfl6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODa60238	XM_017357664.2 Latest version!	Danio rerio EGF-like-domain, multiple 6 (egfl6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODa60239	XM_017357665.1 Latest version!	Danio rerio EGF-like-domain, multiple 6 (egfl6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
ODa60238	XM_017357664.1	Danio rerio EGF-like-domain, multiple 6 (egfl6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

egfl6 ( NM_001002457.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001002457.1 Danio rerio EGF-like-domain, multiple 6 (egfl6), mRNA.

Price & Availability↑

CloneID	ODa03897
Clone ID Related Accession (Same CDS sequence)	NM_001002457.1 , XM_021478639.1
Accession Version	NM_001002457.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556553600000
Organism	Danio rerio(zebrafish)
Product	epidermal growth factor-like protein 6
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC076013.1. ##Evidence-Data-START## Transcript exon combination :: BC076013.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGAGTGGGC TGGGTGTGTG TCGATACGGC AGCCGGTTGG AGTGCTGCTA CGGCTGGAAG 
AAAAACACTA AAGGACAATG TGAAGCTCAG TGTGATCTGG GCTGCAAACA CGGCGAGTGT 
GTCGGCCCCA ACAAATGCAA GTGTTTTCCT GGATACACTG GAAAGACCTG CAGCCAAGAT 
CTGAATGAGT GTGGTCTGAA GCCTCGTCCC TGTGAGCATC GCTGTATGAA CACATTCGGC 
AGCTATATGT GCTACTGCTT AAACGGCTAC ATGCTAATGC CTGATGGATC CTGTGCAAAC 
TCCAGGACGT GCTCTCTGGC TCACTGTCAG TACGGCTGTG AGGAGGTGCA GTCTGAGGTT 
CGCTGTCTCT GTCCATCCCC TGGCCTTCAG CTCGGGTCTG ATGGAAAGAC CTGCGAGGAC 
ATTGATGAAT GTGCGACTGG GAAAAACCAG TGTCCGTTTA ACCGGCAGTG CACAAACACA 
TTCGGCAGCT ACTACTGCAA GTGTCAGCCA GGATACGACC TCAAATACAT CAATGGAAAA 
TACGACTGCG TTGATGTAAA TGAGTGCACG TCGAACACAC ACAAGTGCAG TCATCACGCT 
GAATGCATCA ACACTCTTGG GTCCTACAAG TGTAAATGCA AGCAGGGCTT CCGTGGCAGC 
GGATTCGATT GTTCAGTCAA GCCATTTTAT CACAGATCCT GGGACAGCAG TAAAGGAAAC 
ACAGATGTGC TTGTGAACGC GATTCCCGAT TTTCCGTCCA AAACCCGGAT TTTGGGTAAA 
TTTGAGAACA GCATCCCAGA GCCTGTGCTG ACTGAATCTC CTCGACAGCA TCTGCAACCC 
TTCGATTATG ATGGAGAGGT TTACATCGGC GCTCCACAGG AAACACCAGT GTTTCCTGAG 
GAAGAAGAAG TGGAAGAGGA AGAGGAAGAG GACACAGAGA ACCAGCTGGA GGATGAAGAG 
CTGACCAGCA GAGGAGACGT GTTTTCTCAG GATGATTTTG TGTCTTTTTA TCAATCAGAG 
TTAGAGTTGA AGGAGATCCA GGCGGCGCCG CTGAAGGAAA GATTTCTGAC GGATTGTGAC 
TTTGACCGCG GTGCTTGTGA ATGGGTTCAG GATAACGCAG ATGATCTGGA CTGGACCATC 
AAATATCATC CAGACGGGCG GGAGTATTAC CTGGCTCTCG AGGGCCCCGT GGGGAACAGG 
CGTGAGCAGG CCCGCATGAA GCTCCTGCTG GAAGATCACA TGCGGCAGAG CAGCTTCTGT 
CTGAGGTTTG ATTTCCGCAT TGAGGGTCCG CAGACGGGTG TTTTACGGGT GAAACTGGAC 
AACAGCGCCA TCTCCATCTG GGAGAGAAAA CAGAGTCAGA AGCAGAGCTG GCAGAGCGAG 
CAGATCAGCA TCAGCTGGAC AGACAGTAAT CCTGAAGCGG TGGTCTTTGA GGCTGAGCGG 
GGACGCTCCG GCACTGGAGA AATCGCTCTG GATCATGTGC TGCTGATCTC TGGAGAATGT 
TCAGAAGATA AAACACTCGA CTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001002457.1
CDS	137..1663
Translation	MSGLGVCRYGSRLECCYGWKKNTKGQCEAQCDLGCKHGECVGPNKCKCFPGYTGKTCSQDLNECGLKPRPCEHRCMNTFGSYMCYCLNGYMLMPDGSCANSRTCSLAHCQYGCEEVQSEVRCLCPSPGLQLGSDGKTCEDIDECATGKNQCPFNRQCTNTFGSYYCKCQPGYDLKYINGKYDCVDVNECTSNTHKCSHHAECINTLGSYKCKCKQGFRGSGFDCSVKPFYHRSWDSSKGNTDVLVNAIPDFPSKTRILGKFENSIPEPVLTESPRQHLQPFDYDGEVYIGAPQETPVFPEEEEVEEEEEEDTENQLEDEELTSRGDVFSQDDFVSFYQSELELKEIQAAPLKERFLTDCDFDRGACEWVQDNADDLDWTIKYHPDGREYYLALEGPVGNRREQARMKLLLEDHMRQSSFCLRFDFRIEGPQTGVLRVKLDNSAISIWERKQSQKQSWQSEQISISWTDSNPEAVVFEAERGRSGTGEIALDHVLLISGECSEDKTLDF

Target ORF information:

RefSeq Version	NM_001002457.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio EGF-like-domain, multiple 6 (egfl6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001002457.1

ORF Insert Sequence:

ATGAGTGGGC TGGGTGTGTG TCGATACGGC AGCCGGTTGG AGTGCTGCTA CGGCTGGAAG 
AAAAACACTA AAGGACAATG TGAAGCTCAG TGTGATCTGG GCTGCAAACA CGGCGAGTGT 
GTCGGCCCCA ACAAATGCAA GTGTTTTCCT GGATACACTG GAAAGACCTG CAGCCAAGAT 
CTGAATGAGT GTGGTCTGAA GCCTCGTCCC TGTGAGCATC GCTGTATGAA CACATTCGGC 
AGCTATATGT GCTACTGCTT AAACGGCTAC ATGCTAATGC CTGATGGATC CTGTGCAAAC 
TCCAGGACGT GCTCTCTGGC TCACTGTCAG TACGGCTGTG AGGAGGTGCA GTCTGAGGTT 
CGCTGTCTCT GTCCATCCCC TGGCCTTCAG CTCGGGTCTG ATGGAAAGAC CTGCGAGGAC 
ATTGATGAAT GTGCGACTGG GAAAAACCAG TGTCCGTTTA ACCGGCAGTG CACAAACACA 
TTCGGCAGCT ACTACTGCAA GTGTCAGCCA GGATACGACC TCAAATACAT CAATGGAAAA 
TACGACTGCG TTGATGTAAA TGAGTGCACG TCGAACACAC ACAAGTGCAG TCATCACGCT 
GAATGCATCA ACACTCTTGG GTCCTACAAG TGTAAATGCA AGCAGGGCTT CCGTGGCAGC 
GGATTCGATT GTTCAGTCAA GCCATTTTAT CACAGATCCT GGGACAGCAG TAAAGGAAAC 
ACAGATGTGC TTGTGAACGC GATTCCCGAT TTTCCGTCCA AAACCCGGAT TTTGGGTAAA 
TTTGAGAACA GCATCCCAGA GCCTGTGCTG ACTGAATCTC CTCGACAGCA TCTGCAACCC 
TTCGATTATG ATGGAGAGGT TTACATCGGC GCTCCACAGG AAACACCAGT GTTTCCTGAG 
GAAGAAGAAG TGGAAGAGGA AGAGGAAGAG GACACAGAGA ACCAGCTGGA GGATGAAGAG 
CTGACCAGCA GAGGAGACGT GTTTTCTCAG GATGATTTTG TGTCTTTTTA TCAATCAGAG 
TTAGAGTTGA AGGAGATCCA GGCGGCGCCG CTGAAGGAAA GATTTCTGAC GGATTGTGAC 
TTTGACCGCG GTGCTTGTGA ATGGGTTCAG GATAACGCAG ATGATCTGGA CTGGACCATC 
AAATATCATC CAGACGGGCG GGAGTATTAC CTGGCTCTCG AGGGCCCCGT GGGGAACAGG 
CGTGAGCAGG CCCGCATGAA GCTCCTGCTG GAAGATCACA TGCGGCAGAG CAGCTTCTGT 
CTGAGGTTTG ATTTCCGCAT TGAGGGTCCG CAGACGGGTG TTTTACGGGT GAAACTGGAC 
AACAGCGCCA TCTCCATCTG GGAGAGAAAA CAGAGTCAGA AGCAGAGCTG GCAGAGCGAG 
CAGATCAGCA TCAGCTGGAC AGACAGTAAT CCTGAAGCGG TGGTCTTTGA GGCTGAGCGG 
GGACGCTCCG GCACTGGAGA AATCGCTCTG GATCATGTGC TGCTGATCTC TGGAGAATGT 
TCAGAAGATA AAACACTCGA CTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egfl6 ( XM_021478639.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334314.1 Danio rerio EGF-like-domain, multiple 6 (egfl6), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa03897
Clone ID Related Accession (Same CDS sequence)	NM_001002457.1 , XM_021478639.1
Accession Version	XM_021478639.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	epidermal growth factor-like protein 6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGGC TGGGTGTGTG TCGATACGGC AGCCGGTTGG AGTGCTGCTA CGGCTGGAAG 
AAAAACACTA AAGGACAATG TGAAGCTCAG TGTGATCTGG GCTGCAAACA CGGCGAGTGT 
GTCGGCCCCA ACAAATGCAA GTGTTTTCCT GGATACACTG GAAAGACCTG CAGCCAAGAT 
CTGAATGAGT GTGGTCTGAA GCCTCGTCCC TGTGAGCATC GCTGTATGAA CACATTCGGC 
AGCTATATGT GCTACTGCTT AAACGGCTAC ATGCTAATGC CTGATGGATC CTGTGCAAAC 
TCCAGGACGT GCTCTCTGGC TCACTGTCAG TACGGCTGTG AGGAGGTGCA GTCTGAGGTT 
CGCTGTCTCT GTCCATCCCC TGGCCTTCAG CTCGGGTCTG ATGGAAAGAC CTGCGAGGAC 
ATTGATGAAT GTGCGACTGG GAAAAACCAG TGTCCGTTTA ACCGGCAGTG CACAAACACA 
TTCGGCAGCT ACTACTGCAA GTGTCAGCCA GGATACGACC TCAAATACAT CAATGGAAAA 
TACGACTGCG TTGATGTAAA TGAGTGCACG TCGAACACAC ACAAGTGCAG TCATCACGCT 
GAATGCATCA ACACTCTTGG GTCCTACAAG TGTAAATGCA AGCAGGGCTT CCGTGGCAGC 
GGATTCGATT GTTCAGTCAA GCCATTTTAT CACAGATCCT GGGACAGCAG TAAAGGAAAC 
ACAGATGTGC TTGTGAACGC GATTCCCGAT TTTCCGTCCA AAACCCGGAT TTTGGGTAAA 
TTTGAGAACA GCATCCCAGA GCCTGTGCTG ACTGAATCTC CTCGACAGCA TCTGCAACCC 
TTCGATTATG ATGGAGAGGT TTACATCGGC GCTCCACAGG AAACACCAGT GTTTCCTGAG 
GAAGAAGAAG TGGAAGAGGA AGAGGAAGAG GACACAGAGA ACCAGCTGGA GGATGAAGAG 
CTGACCAGCA GAGGAGACGT GTTTTCTCAG GATGATTTTG TGTCTTTTTA TCAATCAGAG 
TTAGAGTTGA AGGAGATCCA GGCGGCGCCG CTGAAGGAAA GATTTCTGAC GGATTGTGAC 
TTTGACCGCG GTGCTTGTGA ATGGGTTCAG GATAACGCAG ATGATCTGGA CTGGACCATC 
AAATATCATC CAGACGGGCG GGAGTATTAC CTGGCTCTCG AGGGCCCCGT GGGGAACAGG 
CGTGAGCAGG CCCGCATGAA GCTCCTGCTG GAAGATCACA TGCGGCAGAG CAGCTTCTGT 
CTGAGGTTTG ATTTCCGCAT TGAGGGTCCG CAGACGGGTG TTTTACGGGT GAAACTGGAC 
AACAGCGCCA TCTCCATCTG GGAGAGAAAA CAGAGTCAGA AGCAGAGCTG GCAGAGCGAG 
CAGATCAGCA TCAGCTGGAC AGACAGTAAT CCTGAAGCGG TGGTCTTTGA GGCTGAGCGG 
GGACGCTCCG GCACTGGAGA AATCGCTCTG GATCATGTGC TGCTGATCTC TGGAGAATGT 
TCAGAAGATA AAACACTCGA CTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334314.1
CDS	47..1573
Translation	MSGLGVCRYGSRLECCYGWKKNTKGQCEAQCDLGCKHGECVGPNKCKCFPGYTGKTCSQDLNECGLKPRPCEHRCMNTFGSYMCYCLNGYMLMPDGSCANSRTCSLAHCQYGCEEVQSEVRCLCPSPGLQLGSDGKTCEDIDECATGKNQCPFNRQCTNTFGSYYCKCQPGYDLKYINGKYDCVDVNECTSNTHKCSHHAECINTLGSYKCKCKQGFRGSGFDCSVKPFYHRSWDSSKGNTDVLVNAIPDFPSKTRILGKFENSIPEPVLTESPRQHLQPFDYDGEVYIGAPQETPVFPEEEEVEEEEEEDTENQLEDEELTSRGDVFSQDDFVSFYQSELELKEIQAAPLKERFLTDCDFDRGACEWVQDNADDLDWTIKYHPDGREYYLALEGPVGNRREQARMKLLLEDHMRQSSFCLRFDFRIEGPQTGVLRVKLDNSAISIWERKQSQKQSWQSEQISISWTDSNPEAVVFEAERGRSGTGEIALDHVLLISGECSEDKTLDF

Target ORF information:

RefSeq Version	XM_021478639.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio EGF-like-domain, multiple 6 (egfl6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478639.1

ORF Insert Sequence:

ATGAGTGGGC TGGGTGTGTG TCGATACGGC AGCCGGTTGG AGTGCTGCTA CGGCTGGAAG 
AAAAACACTA AAGGACAATG TGAAGCTCAG TGTGATCTGG GCTGCAAACA CGGCGAGTGT 
GTCGGCCCCA ACAAATGCAA GTGTTTTCCT GGATACACTG GAAAGACCTG CAGCCAAGAT 
CTGAATGAGT GTGGTCTGAA GCCTCGTCCC TGTGAGCATC GCTGTATGAA CACATTCGGC 
AGCTATATGT GCTACTGCTT AAACGGCTAC ATGCTAATGC CTGATGGATC CTGTGCAAAC 
TCCAGGACGT GCTCTCTGGC TCACTGTCAG TACGGCTGTG AGGAGGTGCA GTCTGAGGTT 
CGCTGTCTCT GTCCATCCCC TGGCCTTCAG CTCGGGTCTG ATGGAAAGAC CTGCGAGGAC 
ATTGATGAAT GTGCGACTGG GAAAAACCAG TGTCCGTTTA ACCGGCAGTG CACAAACACA 
TTCGGCAGCT ACTACTGCAA GTGTCAGCCA GGATACGACC TCAAATACAT CAATGGAAAA 
TACGACTGCG TTGATGTAAA TGAGTGCACG TCGAACACAC ACAAGTGCAG TCATCACGCT 
GAATGCATCA ACACTCTTGG GTCCTACAAG TGTAAATGCA AGCAGGGCTT CCGTGGCAGC 
GGATTCGATT GTTCAGTCAA GCCATTTTAT CACAGATCCT GGGACAGCAG TAAAGGAAAC 
ACAGATGTGC TTGTGAACGC GATTCCCGAT TTTCCGTCCA AAACCCGGAT TTTGGGTAAA 
TTTGAGAACA GCATCCCAGA GCCTGTGCTG ACTGAATCTC CTCGACAGCA TCTGCAACCC 
TTCGATTATG ATGGAGAGGT TTACATCGGC GCTCCACAGG AAACACCAGT GTTTCCTGAG 
GAAGAAGAAG TGGAAGAGGA AGAGGAAGAG GACACAGAGA ACCAGCTGGA GGATGAAGAG 
CTGACCAGCA GAGGAGACGT GTTTTCTCAG GATGATTTTG TGTCTTTTTA TCAATCAGAG 
TTAGAGTTGA AGGAGATCCA GGCGGCGCCG CTGAAGGAAA GATTTCTGAC GGATTGTGAC 
TTTGACCGCG GTGCTTGTGA ATGGGTTCAG GATAACGCAG ATGATCTGGA CTGGACCATC 
AAATATCATC CAGACGGGCG GGAGTATTAC CTGGCTCTCG AGGGCCCCGT GGGGAACAGG 
CGTGAGCAGG CCCGCATGAA GCTCCTGCTG GAAGATCACA TGCGGCAGAG CAGCTTCTGT 
CTGAGGTTTG ATTTCCGCAT TGAGGGTCCG CAGACGGGTG TTTTACGGGT GAAACTGGAC 
AACAGCGCCA TCTCCATCTG GGAGAGAAAA CAGAGTCAGA AGCAGAGCTG GCAGAGCGAG 
CAGATCAGCA TCAGCTGGAC AGACAGTAAT CCTGAAGCGG TGGTCTTTGA GGCTGAGCGG 
GGACGCTCCG GCACTGGAGA AATCGCTCTG GATCATGTGC TGCTGATCTC TGGAGAATGT 
TCAGAAGATA AAACACTCGA CTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egfl6 ( XM_017357664.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213153.1 Danio rerio EGF-like-domain, multiple 6 (egfl6), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa60238
Clone ID Related Accession (Same CDS sequence)	XM_017357664.1 , XM_017357664.2
Accession Version	XM_017357664.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	epidermal growth factor-like protein 6 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACATC TGACGTGGAT CAGCGCTCTC TCACTCCTGC TGTCTTTTAC ACTAGGAACT 
GCAGATCACA GACAGCGGCG GCAGATCTCC GTCATGAGTG GGCTGGGTGT GTGTCGATAC 
GGCAGCCGGT TGGAGTGCTG CTACGGCTGG AAGAAAAACA CTAAAGGACA ATGTGAAGCT 
CAGTGTGATC TGGGCTGCAA ACACGGCGAG TGTGTCGGCC CCAACAAATG CAAGTGTTTT 
CCTGGATACA CTGGAAAGAC CTGCAGCCAA GATCTGAATG AGTGTGGTCT GAAGCCTCGT 
CCCTGTGAGC ATCGCTGTAT GAACACATTC GGCAGCTATA TGTGCTACTG CTTAAACGGC 
TACATGCTAA TGCCTGATGG ATCCTGTGCA AACTCCAGGA CGTGCTCTCT GGCTCACTGT 
CAGTACGGCT GTGAGGAGGT GCAGTCTGAG GTTCGCTGTC TCTGTCCATC CCCTGGCCTT 
CAGCTCGGGT CTGATGGAAA GACCTGCGAG GACATTGATG AATGTGCGAC TGGGAAAAAC 
CAGTGTCCGT TTAACCGGCA GTGCACAAAC ACATTCGGCA GCTACTACTG CAAGTGTCAG 
CCAGGATACG ACCTCAAATA CATCAATGGA AAATACGACT GCGTTGATGT AAATGAGTGC 
ACGTCGAACA CACACAAGTG CAGTCATCAC GCTGAATGCA TCAACACTCT TGGGTCCTAC 
AAGTGTAAAT GCAAGCAGGG CTTCCGTGGC AGCGGATTCG ATTGTTCAGT CAAGCCATTT 
TATCACAGAT CCTGGGACAG CAGTAAAGGA AACACAGATG TGCTTGTGAA CGCGATTCCC 
GATTTTCCGT CCAAAACCCG GATTTTGGGT AAATTTGAGA ACAGCATCCC AGAGCCTGTG 
CTGACTGAAT CTCCTCGACA GCATCTGCAA CCCTTCGATT ATGATGGAGA GGTTTACATC 
GGCGCTCCAC AGGAAACACC AGTGTTTCCT GAGGAAGAAG AAGTGGAAGA GGAAGAGGAA 
GAGGACACAG AGAACCAGCT GGAGGATGAA GAGCTGACCA GCAGAGGAGA CGTGTTTTCT 
CAGGATGATT TTGTGTCTTT TTATCAATCA GAGTTAGAGT TGAAGGAGAT CCAGGCGGCG 
CCGCTGAAGG AAAGATTTCT GACGGATTGT GACTTTGACC GCGGTGCTTG TGAATGGGTT 
CAGGATAACG CAGATGATCT GGACTGGACC ATCAAATATC ATCCAGACGG GCGGGAGTAT 
TACCTGGCTC TCGAGGGCCC CGTGGGGAAC AGGCGTGAGC AGGCCCGCAT GAAGCTCCTG 
CTGGAAGATC ACATGCGGCA GAGCAGCTTC TGTCTGAGGT TTGATTTCCG CATTGAGGGT 
CCGCAGACGG GTGTTTTACG GGTGAAACTG GACAACAGCG CCATCTCCAT CTGGGAGAGA 
AAACAGAGTC AGAAGCAGAG CTGGCAGAGC GAGCAGATCA GCATCAGCTG GACAGACAGT 
AATCCTGAAG CGGTGGTCTT TGAGGCTGAG CGGGGACGCT CCGGCACTGG AGAAATCGCT 
CTGGATCATG TGCTGCTGAT CTCTGGAGAA TGTTCAGAAG ATAAAACACT CGACTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213153.1
CDS	103..1722
Translation	MKHLTWISALSLLLSFTLGTADHRQRRQISVMSGLGVCRYGSRLECCYGWKKNTKGQCEAQCDLGCKHGECVGPNKCKCFPGYTGKTCSQDLNECGLKPRPCEHRCMNTFGSYMCYCLNGYMLMPDGSCANSRTCSLAHCQYGCEEVQSEVRCLCPSPGLQLGSDGKTCEDIDECATGKNQCPFNRQCTNTFGSYYCKCQPGYDLKYINGKYDCVDVNECTSNTHKCSHHAECINTLGSYKCKCKQGFRGSGFDCSVKPFYHRSWDSSKGNTDVLVNAIPDFPSKTRILGKFENSIPEPVLTESPRQHLQPFDYDGEVYIGAPQETPVFPEEEEVEEEEEEDTENQLEDEELTSRGDVFSQDDFVSFYQSELELKEIQAAPLKERFLTDCDFDRGACEWVQDNADDLDWTIKYHPDGREYYLALEGPVGNRREQARMKLLLEDHMRQSSFCLRFDFRIEGPQTGVLRVKLDNSAISIWERKQSQKQSWQSEQISISWTDSNPEAVVFEAERGRSGTGEIALDHVLLISGECSEDKTLDF

Target ORF information:

RefSeq Version	XM_017357664.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio EGF-like-domain, multiple 6 (egfl6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357664.1

ORF Insert Sequence:

ATGAAACATC TGACGTGGAT CAGCGCTCTC TCACTCCTGC TGTCTTTTAC ACTAGGAACT 
GCAGATCACA GACAGCGGCG GCAGATCTCC GTCATGAGTG GGCTGGGTGT GTGTCGATAC 
GGCAGCCGGT TGGAGTGCTG CTACGGCTGG AAGAAAAACA CTAAAGGACA ATGTGAAGCT 
CAGTGTGATC TGGGCTGCAA ACACGGCGAG TGTGTCGGCC CCAACAAATG CAAGTGTTTT 
CCTGGATACA CTGGAAAGAC CTGCAGCCAA GATCTGAATG AGTGTGGTCT GAAGCCTCGT 
CCCTGTGAGC ATCGCTGTAT GAACACATTC GGCAGCTATA TGTGCTACTG CTTAAACGGC 
TACATGCTAA TGCCTGATGG ATCCTGTGCA AACTCCAGGA CGTGCTCTCT GGCTCACTGT 
CAGTACGGCT GTGAGGAGGT GCAGTCTGAG GTTCGCTGTC TCTGTCCATC CCCTGGCCTT 
CAGCTCGGGT CTGATGGAAA GACCTGCGAG GACATTGATG AATGTGCGAC TGGGAAAAAC 
CAGTGTCCGT TTAACCGGCA GTGCACAAAC ACATTCGGCA GCTACTACTG CAAGTGTCAG 
CCAGGATACG ACCTCAAATA CATCAATGGA AAATACGACT GCGTTGATGT AAATGAGTGC 
ACGTCGAACA CACACAAGTG CAGTCATCAC GCTGAATGCA TCAACACTCT TGGGTCCTAC 
AAGTGTAAAT GCAAGCAGGG CTTCCGTGGC AGCGGATTCG ATTGTTCAGT CAAGCCATTT 
TATCACAGAT CCTGGGACAG CAGTAAAGGA AACACAGATG TGCTTGTGAA CGCGATTCCC 
GATTTTCCGT CCAAAACCCG GATTTTGGGT AAATTTGAGA ACAGCATCCC AGAGCCTGTG 
CTGACTGAAT CTCCTCGACA GCATCTGCAA CCCTTCGATT ATGATGGAGA GGTTTACATC 
GGCGCTCCAC AGGAAACACC AGTGTTTCCT GAGGAAGAAG AAGTGGAAGA GGAAGAGGAA 
GAGGACACAG AGAACCAGCT GGAGGATGAA GAGCTGACCA GCAGAGGAGA CGTGTTTTCT 
CAGGATGATT TTGTGTCTTT TTATCAATCA GAGTTAGAGT TGAAGGAGAT CCAGGCGGCG 
CCGCTGAAGG AAAGATTTCT GACGGATTGT GACTTTGACC GCGGTGCTTG TGAATGGGTT 
CAGGATAACG CAGATGATCT GGACTGGACC ATCAAATATC ATCCAGACGG GCGGGAGTAT 
TACCTGGCTC TCGAGGGCCC CGTGGGGAAC AGGCGTGAGC AGGCCCGCAT GAAGCTCCTG 
CTGGAAGATC ACATGCGGCA GAGCAGCTTC TGTCTGAGGT TTGATTTCCG CATTGAGGGT 
CCGCAGACGG GTGTTTTACG GGTGAAACTG GACAACAGCG CCATCTCCAT CTGGGAGAGA 
AAACAGAGTC AGAAGCAGAG CTGGCAGAGC GAGCAGATCA GCATCAGCTG GACAGACAGT 
AATCCTGAAG CGGTGGTCTT TGAGGCTGAG CGGGGACGCT CCGGCACTGG AGAAATCGCT 
CTGGATCATG TGCTGCTGAT CTCTGGAGAA TGTTCAGAAG ATAAAACACT CGACTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egfl6 ( XM_017357664.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213153.1 Danio rerio EGF-like-domain, multiple 6 (egfl6), transcript variant X1, mRNA.

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CloneID	ODa60238
Clone ID Related Accession (Same CDS sequence)	XM_017357664.1 , XM_017357664.2
Accession Version	XM_017357664.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	epidermal growth factor-like protein 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017357664.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACATC TGACGTGGAT CAGCGCTCTC TCACTCCTGC TGTCTTTTAC ACTAGGAACT 
GCAGATCACA GACAGCGGCG GCAGATCTCC GTCATGAGTG GGCTGGGTGT GTGTCGATAC 
GGCAGCCGGT TGGAGTGCTG CTACGGCTGG AAGAAAAACA CTAAAGGACA ATGTGAAGCT 
CAGTGTGATC TGGGCTGCAA ACACGGCGAG TGTGTCGGCC CCAACAAATG CAAGTGTTTT 
CCTGGATACA CTGGAAAGAC CTGCAGCCAA GATCTGAATG AGTGTGGTCT GAAGCCTCGT 
CCCTGTGAGC ATCGCTGTAT GAACACATTC GGCAGCTATA TGTGCTACTG CTTAAACGGC 
TACATGCTAA TGCCTGATGG ATCCTGTGCA AACTCCAGGA CGTGCTCTCT GGCTCACTGT 
CAGTACGGCT GTGAGGAGGT GCAGTCTGAG GTTCGCTGTC TCTGTCCATC CCCTGGCCTT 
CAGCTCGGGT CTGATGGAAA GACCTGCGAG GACATTGATG AATGTGCGAC TGGGAAAAAC 
CAGTGTCCGT TTAACCGGCA GTGCACAAAC ACATTCGGCA GCTACTACTG CAAGTGTCAG 
CCAGGATACG ACCTCAAATA CATCAATGGA AAATACGACT GCGTTGATGT AAATGAGTGC 
ACGTCGAACA CACACAAGTG CAGTCATCAC GCTGAATGCA TCAACACTCT TGGGTCCTAC 
AAGTGTAAAT GCAAGCAGGG CTTCCGTGGC AGCGGATTCG ATTGTTCAGT CAAGCCATTT 
TATCACAGAT CCTGGGACAG CAGTAAAGGA AACACAGATG TGCTTGTGAA CGCGATTCCC 
GATTTTCCGT CCAAAACCCG GATTTTGGGT AAATTTGAGA ACAGCATCCC AGAGCCTGTG 
CTGACTGAAT CTCCTCGACA GCATCTGCAA CCCTTCGATT ATGATGGAGA GGTTTACATC 
GGCGCTCCAC AGGAAACACC AGTGTTTCCT GAGGAAGAAG AAGTGGAAGA GGAAGAGGAA 
GAGGACACAG AGAACCAGCT GGAGGATGAA GAGCTGACCA GCAGAGGAGA CGTGTTTTCT 
CAGGATGATT TTGTGTCTTT TTATCAATCA GAGTTAGAGT TGAAGGAGAT CCAGGCGGCG 
CCGCTGAAGG AAAGATTTCT GACGGATTGT GACTTTGACC GCGGTGCTTG TGAATGGGTT 
CAGGATAACG CAGATGATCT GGACTGGACC ATCAAATATC ATCCAGACGG GCGGGAGTAT 
TACCTGGCTC TCGAGGGCCC CGTGGGGAAC AGGCGTGAGC AGGCCCGCAT GAAGCTCCTG 
CTGGAAGATC ACATGCGGCA GAGCAGCTTC TGTCTGAGGT TTGATTTCCG CATTGAGGGT 
CCGCAGACGG GTGTTTTACG GGTGAAACTG GACAACAGCG CCATCTCCAT CTGGGAGAGA 
AAACAGAGTC AGAAGCAGAG CTGGCAGAGC GAGCAGATCA GCATCAGCTG GACAGACAGT 
AATCCTGAAG CGGTGGTCTT TGAGGCTGAG CGGGGACGCT CCGGCACTGG AGAAATCGCT 
CTGGATCATG TGCTGCTGAT CTCTGGAGAA TGTTCAGAAG ATAAAACACT CGACTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213153.1
CDS	117..1736
Translation	MKHLTWISALSLLLSFTLGTADHRQRRQISVMSGLGVCRYGSRLECCYGWKKNTKGQCEAQCDLGCKHGECVGPNKCKCFPGYTGKTCSQDLNECGLKPRPCEHRCMNTFGSYMCYCLNGYMLMPDGSCANSRTCSLAHCQYGCEEVQSEVRCLCPSPGLQLGSDGKTCEDIDECATGKNQCPFNRQCTNTFGSYYCKCQPGYDLKYINGKYDCVDVNECTSNTHKCSHHAECINTLGSYKCKCKQGFRGSGFDCSVKPFYHRSWDSSKGNTDVLVNAIPDFPSKTRILGKFENSIPEPVLTESPRQHLQPFDYDGEVYIGAPQETPVFPEEEEVEEEEEEDTENQLEDEELTSRGDVFSQDDFVSFYQSELELKEIQAAPLKERFLTDCDFDRGACEWVQDNADDLDWTIKYHPDGREYYLALEGPVGNRREQARMKLLLEDHMRQSSFCLRFDFRIEGPQTGVLRVKLDNSAISIWERKQSQKQSWQSEQISISWTDSNPEAVVFEAERGRSGTGEIALDHVLLISGECSEDKTLDF

Target ORF information:

RefSeq Version	XM_017357664.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio EGF-like-domain, multiple 6 (egfl6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357664.2

ORF Insert Sequence:

ATGAAACATC TGACGTGGAT CAGCGCTCTC TCACTCCTGC TGTCTTTTAC ACTAGGAACT 
GCAGATCACA GACAGCGGCG GCAGATCTCC GTCATGAGTG GGCTGGGTGT GTGTCGATAC 
GGCAGCCGGT TGGAGTGCTG CTACGGCTGG AAGAAAAACA CTAAAGGACA ATGTGAAGCT 
CAGTGTGATC TGGGCTGCAA ACACGGCGAG TGTGTCGGCC CCAACAAATG CAAGTGTTTT 
CCTGGATACA CTGGAAAGAC CTGCAGCCAA GATCTGAATG AGTGTGGTCT GAAGCCTCGT 
CCCTGTGAGC ATCGCTGTAT GAACACATTC GGCAGCTATA TGTGCTACTG CTTAAACGGC 
TACATGCTAA TGCCTGATGG ATCCTGTGCA AACTCCAGGA CGTGCTCTCT GGCTCACTGT 
CAGTACGGCT GTGAGGAGGT GCAGTCTGAG GTTCGCTGTC TCTGTCCATC CCCTGGCCTT 
CAGCTCGGGT CTGATGGAAA GACCTGCGAG GACATTGATG AATGTGCGAC TGGGAAAAAC 
CAGTGTCCGT TTAACCGGCA GTGCACAAAC ACATTCGGCA GCTACTACTG CAAGTGTCAG 
CCAGGATACG ACCTCAAATA CATCAATGGA AAATACGACT GCGTTGATGT AAATGAGTGC 
ACGTCGAACA CACACAAGTG CAGTCATCAC GCTGAATGCA TCAACACTCT TGGGTCCTAC 
AAGTGTAAAT GCAAGCAGGG CTTCCGTGGC AGCGGATTCG ATTGTTCAGT CAAGCCATTT 
TATCACAGAT CCTGGGACAG CAGTAAAGGA AACACAGATG TGCTTGTGAA CGCGATTCCC 
GATTTTCCGT CCAAAACCCG GATTTTGGGT AAATTTGAGA ACAGCATCCC AGAGCCTGTG 
CTGACTGAAT CTCCTCGACA GCATCTGCAA CCCTTCGATT ATGATGGAGA GGTTTACATC 
GGCGCTCCAC AGGAAACACC AGTGTTTCCT GAGGAAGAAG AAGTGGAAGA GGAAGAGGAA 
GAGGACACAG AGAACCAGCT GGAGGATGAA GAGCTGACCA GCAGAGGAGA CGTGTTTTCT 
CAGGATGATT TTGTGTCTTT TTATCAATCA GAGTTAGAGT TGAAGGAGAT CCAGGCGGCG 
CCGCTGAAGG AAAGATTTCT GACGGATTGT GACTTTGACC GCGGTGCTTG TGAATGGGTT 
CAGGATAACG CAGATGATCT GGACTGGACC ATCAAATATC ATCCAGACGG GCGGGAGTAT 
TACCTGGCTC TCGAGGGCCC CGTGGGGAAC AGGCGTGAGC AGGCCCGCAT GAAGCTCCTG 
CTGGAAGATC ACATGCGGCA GAGCAGCTTC TGTCTGAGGT TTGATTTCCG CATTGAGGGT 
CCGCAGACGG GTGTTTTACG GGTGAAACTG GACAACAGCG CCATCTCCAT CTGGGAGAGA 
AAACAGAGTC AGAAGCAGAG CTGGCAGAGC GAGCAGATCA GCATCAGCTG GACAGACAGT 
AATCCTGAAG CGGTGGTCTT TGAGGCTGAG CGGGGACGCT CCGGCACTGG AGAAATCGCT 
CTGGATCATG TGCTGCTGAT CTCTGGAGAA TGTTCAGAAG ATAAAACACT CGACTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egfl6 ( XM_017357665.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213154.1 Danio rerio EGF-like-domain, multiple 6 (egfl6), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa60239
Clone ID Related Accession (Same CDS sequence)	XM_017357665.1
Accession Version	XM_017357665.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	epidermal growth factor-like protein 6 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACATC TGACGTGGAT CAGCGCTCTC TCACTCCTGC TGTCTTTTAC ACTAGGAACT 
GCAGATCACA GACAGCGGCG GCAGATCTCC GTCATGAGTG GGCTGGGTGT GTGTCGATAC 
GGCAGCCGGT TGGAGTGCTG CTACGGCTGG AAGAAAAACA CTAAAGGACA ATGTGAAGCT 
CAGTGTGATC TGGGCTGCAA ACACGGCGAG TGTGTCGGCC CCAACAAATG CAAGTGTTTT 
CCTGGATACA CTGGAAAGAC CTGCAGCCAA GATCTGAATG AGTGTGGTCT GAAGCCTCGT 
CCCTGTGAGC ATCGCTGTAT GAACACATTC GGCAGCTATA TGTGCTACTG CTTAAACGGC 
TACATGCTAA TGCCTGATGG ATCCTGTGCA AACTCCAGGA CGTGCTCTCT GGCTCACTGT 
CAGTACGGCT GTGAGGAGGT GCAGTCTGAG GTTCGCTGTC TCTGTCCATC CCCTGGCCTT 
CAGCTCGGGT CTGATGGAAA GACCTGCGAG GACATTGATG AATGTGCGAC TGGGAAAAAC 
CAGTGTCCGT TTAACCGGCA GTGCACAAAC ACATTCGGCA GCTACTACTG CAAGTGTCAG 
CCAGGATACG ACCTCAAATA CATCAATGGA AAATACGACT GCGTTGATGT AAATGAGTGC 
ACGTCGAACA CACACAAGTG CAGTCATCAC GCTGAATGCA TCAACACTCT TGGGTCCTAC 
AAGTGTAAAT GCAAGCAGGG CTTCCGTGGC AGCGGATTCG ATTGTTCAGC GATTCCCGAT 
TTTCCGTCCA AAACCCGGAT TTTGGGTAAA TTTGAGAACA GCATCCCAGA GCCTGTGCTG 
ACTGAATCTC CTCGACAGCA TCTGCAACCC TTCGATTATG ATGGAGAGGT TTACATCGGC 
GCTCCACAGG AAACACCAGT GTTTCCTGAG GAAGAAGAAG TGGAAGAGGA AGAGGAAGAG 
GACACAGAGA ACCAGCTGGA GGATGAAGAG CTGACCAGCA GAGGAGACGT GTTTTCTCAG 
GATGATTTTG TGTCTTTTTA TCAATCAGAG TTAGAGTTGA AGGAGATCCA GGCGGCGCCG 
CTGAAGGAAA GATTTCTGAC GGATTGTGAC TTTGACCGCG GTGCTTGTGA ATGGGTTCAG 
GATAACGCAG ATGATCTGGA CTGGACCATC AAATATCATC CAGACGGGCG GGAGTATTAC 
CTGGCTCTCG AGGGCCCCGT GGGGAACAGG CGTGAGCAGG CCCGCATGAA GCTCCTGCTG 
GAAGATCACA TGCGGCAGAG CAGCTTCTGT CTGAGGTTTG ATTTCCGCAT TGAGGGTCCG 
CAGACGGGTG TTTTACGGGT GAAACTGGAC AACAGCGCCA TCTCCATCTG GGAGAGAAAA 
CAGAGTCAGA AGCAGAGCTG GCAGAGCGAG CAGATCAGCA TCAGCTGGAC AGACAGTAAT 
CCTGAAGCGG TGGTCTTTGA GGCTGAGCGG GGACGCTCCG GCACTGGAGA AATCGCTCTG 
GATCATGTGC TGCTGATCTC TGGAGAATGT TCAGAAGATA AAACACTCGA CTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213154.1
CDS	103..1659
Translation	MKHLTWISALSLLLSFTLGTADHRQRRQISVMSGLGVCRYGSRLECCYGWKKNTKGQCEAQCDLGCKHGECVGPNKCKCFPGYTGKTCSQDLNECGLKPRPCEHRCMNTFGSYMCYCLNGYMLMPDGSCANSRTCSLAHCQYGCEEVQSEVRCLCPSPGLQLGSDGKTCEDIDECATGKNQCPFNRQCTNTFGSYYCKCQPGYDLKYINGKYDCVDVNECTSNTHKCSHHAECINTLGSYKCKCKQGFRGSGFDCSAIPDFPSKTRILGKFENSIPEPVLTESPRQHLQPFDYDGEVYIGAPQETPVFPEEEEVEEEEEEDTENQLEDEELTSRGDVFSQDDFVSFYQSELELKEIQAAPLKERFLTDCDFDRGACEWVQDNADDLDWTIKYHPDGREYYLALEGPVGNRREQARMKLLLEDHMRQSSFCLRFDFRIEGPQTGVLRVKLDNSAISIWERKQSQKQSWQSEQISISWTDSNPEAVVFEAERGRSGTGEIALDHVLLISGECSEDKTLDF

Target ORF information:

RefSeq Version	XM_017357665.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio EGF-like-domain, multiple 6 (egfl6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357665.1

ORF Insert Sequence:

ATGAAACATC TGACGTGGAT CAGCGCTCTC TCACTCCTGC TGTCTTTTAC ACTAGGAACT 
GCAGATCACA GACAGCGGCG GCAGATCTCC GTCATGAGTG GGCTGGGTGT GTGTCGATAC 
GGCAGCCGGT TGGAGTGCTG CTACGGCTGG AAGAAAAACA CTAAAGGACA ATGTGAAGCT 
CAGTGTGATC TGGGCTGCAA ACACGGCGAG TGTGTCGGCC CCAACAAATG CAAGTGTTTT 
CCTGGATACA CTGGAAAGAC CTGCAGCCAA GATCTGAATG AGTGTGGTCT GAAGCCTCGT 
CCCTGTGAGC ATCGCTGTAT GAACACATTC GGCAGCTATA TGTGCTACTG CTTAAACGGC 
TACATGCTAA TGCCTGATGG ATCCTGTGCA AACTCCAGGA CGTGCTCTCT GGCTCACTGT 
CAGTACGGCT GTGAGGAGGT GCAGTCTGAG GTTCGCTGTC TCTGTCCATC CCCTGGCCTT 
CAGCTCGGGT CTGATGGAAA GACCTGCGAG GACATTGATG AATGTGCGAC TGGGAAAAAC 
CAGTGTCCGT TTAACCGGCA GTGCACAAAC ACATTCGGCA GCTACTACTG CAAGTGTCAG 
CCAGGATACG ACCTCAAATA CATCAATGGA AAATACGACT GCGTTGATGT AAATGAGTGC 
ACGTCGAACA CACACAAGTG CAGTCATCAC GCTGAATGCA TCAACACTCT TGGGTCCTAC 
AAGTGTAAAT GCAAGCAGGG CTTCCGTGGC AGCGGATTCG ATTGTTCAGC GATTCCCGAT 
TTTCCGTCCA AAACCCGGAT TTTGGGTAAA TTTGAGAACA GCATCCCAGA GCCTGTGCTG 
ACTGAATCTC CTCGACAGCA TCTGCAACCC TTCGATTATG ATGGAGAGGT TTACATCGGC 
GCTCCACAGG AAACACCAGT GTTTCCTGAG GAAGAAGAAG TGGAAGAGGA AGAGGAAGAG 
GACACAGAGA ACCAGCTGGA GGATGAAGAG CTGACCAGCA GAGGAGACGT GTTTTCTCAG 
GATGATTTTG TGTCTTTTTA TCAATCAGAG TTAGAGTTGA AGGAGATCCA GGCGGCGCCG 
CTGAAGGAAA GATTTCTGAC GGATTGTGAC TTTGACCGCG GTGCTTGTGA ATGGGTTCAG 
GATAACGCAG ATGATCTGGA CTGGACCATC AAATATCATC CAGACGGGCG GGAGTATTAC 
CTGGCTCTCG AGGGCCCCGT GGGGAACAGG CGTGAGCAGG CCCGCATGAA GCTCCTGCTG 
GAAGATCACA TGCGGCAGAG CAGCTTCTGT CTGAGGTTTG ATTTCCGCAT TGAGGGTCCG 
CAGACGGGTG TTTTACGGGT GAAACTGGAC AACAGCGCCA TCTCCATCTG GGAGAGAAAA 
CAGAGTCAGA AGCAGAGCTG GCAGAGCGAG CAGATCAGCA TCAGCTGGAC AGACAGTAAT 
CCTGAAGCGG TGGTCTTTGA GGCTGAGCGG GGACGCTCCG GCACTGGAGA AATCGCTCTG 
GATCATGTGC TGCTGATCTC TGGAGAATGT TCAGAAGATA AAACACTCGA CTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Analysis of novel caudal hindbrain genes reveals different regulatory logic for gene expression in rhombomere 4 versus 5/6 in embryonic zebrafish.
Neural development13(1)13(2018 06)
Ghosh P,Maurer JM,Sagerström CG

The somite-secreted factor Maeg promotes zebrafish embryonic angiogenesis.
Oncotarget7(47)77749-77763(2016 Nov)
Wang X,Yuan W,Wang X,Qi J,Qin Y,Shi Y,Zhang J,Gong J,Dong Z,Liu X,Sun C,Chai R,Le Noble F,Liu D

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Egfl6 is involved in zebrafish notochord development.
Fish physiology and biochemistry41(4)961-9(2015 Aug)
Wang X,Wang X,Yuan W,Chai R,Liu D

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egfl6 cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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