cpsf1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	cpsf1
Entrez Gene ID	432372
Full Name	cleavage and polyadenylation specific factor 1
Synonyms	wu:fb24c01
General protein information	Preferred Names cleavage and polyadenylation specific factor 1 Names cleavage and polyadenylation specificity factor subunit 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005159584.3	XP_005159641.1	cleavage and polyadenylation specificity factor subunit 1 isoform X1
XM_005159584.4	XP_005159641.1	cleavage and polyadenylation specificity factor subunit 1 isoform X1
NM_001114681.2	NP_001108153.2	cleavage and polyadenylation specificity factor subunit 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CPSF1	XP_003954555.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011340	XP_309311.4
Mus musculus (house mouse)	Cpsf1	NP_444423.1
Drosophila melanogaster (fruit fly)	Cpsf160	NP_995833.1
Canis lupus familiaris (dog)	CPSF1	XP_005628371.1
Oryza sativa (rice)	Os04g0252200	NP_001173813.1
Danio rerio (zebrafish)	cpsf1	NP_001108153.2
Caenorhabditis elegans (roundworm)	cpsf-1	NP_500157.2
Xenopus tropicalis (tropical clawed frog)	cpsf1	XP_002943254.2
Homo sapiens (human)	CPSF1	NP_037423.2
Bos taurus (cattle)	CPSF1	NP_777145.1
Rattus norvegicus (Norway rat)	Cpsf1	NP_001124043.1
Arabidopsis thaliana (thale cress)	CPSF160	NP_199979.2

Gene Ontology
Bibliography

Related articles in PubMed

CPSF1 mutations are associated with early-onset high myopia and involved in retinal ganglion cell axon projection.
Ouyang J, Sun W, Xiao X, Li S, Jia X, Zhou L, Wang P, Zhang Q
Human molecular genetics28(12)1959-1970(2019 06)

Single-cell transcriptional analysis of normal, aberrant, and malignant hematopoiesis in zebrafish.
Moore FE, Garcia EG, Lobbardi R, Jain E, Tang Q, Moore JC, Cortes M, Molodtsov A, Kasheta M, Luo CC, Garcia AJ, Mylvaganam R, Yoder JA, Blackburn JS, Sadreyev RI, Ceol CJ, North TE, Langenau DM
The Journal of experimental medicine213(6)979-92(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

cpsf1 is required for definitive HSC survival in zebrafish.
Bolli N, Payne EM, Rhodes J, Gjini E, Johnston AB, Guo F, Lee JS, Stewart RA, Kanki JP, Chen AT, Zhou Y, Zon LI, Look AT
Blood117(15)3996-4007(2011 Apr)

Identification of 315 genes essential for early zebrafish development.
Amsterdam A, Nissen RM, Sun Z, Swindell EC, Farrington S, Hopkins N
Proceedings of the National Academy of Sciences of the United States of America101(35)12792-7(2004 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following cpsf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cpsf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa34633	NM_001114681.2 Latest version!	Danio rerio cleavage and polyadenylation specific factor 1 (cpsf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa23997	XM_005159584.4 Latest version!	Danio rerio cleavage and polyadenylation specific factor 1 (cpsf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
View Old Accession Versions >>
ODa23997	XM_005159584.3	Danio rerio cleavage and polyadenylation specific factor 1 (cpsf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cpsf1 ( NM_001114681.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001108153.2 Danio rerio cleavage and polyadenylation specific factor 1 (cpsf1), mRNA.

Price & Availability↑

CloneID	ODa34633
Clone ID Related Accession (Same CDS sequence)	NM_001114681.2
Accession Version	NM_001114681.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1573747200000
Organism	Danio rerio(zebrafish)
Product	cleavage and polyadenylation specificity factor subunit 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CU467825.13. On May 1, 2009 this sequence version replaced NM_001114681.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTACGCGG TGTACCGTCA GGCTCACCCG CCCACCGCAG TGGAGTTTGC CGTTTACTGC 
AACTTCATCT CCAGCCAAGA GAAAAACTTG GTGGTGGCAG GAACATCTCA GCTGTACGTC 
TACAGGATCA TTTATGATGT GGAGAGCACT TCCAAGTCTG AAAAGTCATC AGATGGCAAG 
AGTCGTAAAG AGAAACTGGA GCAGGTGGCA TCTTTCTCTC TCTTTGGAAA TGTGATGTCA 
ATGGCCAGCG TTCAGCTGGT GGGCACCAAC AGAGACGCCT TGCTTCTCAG CTTCAAAGAT 
GCCAAGCTGT CAGTAGTGGA GTATGACCCT GGCACGCATG ATCTCAAGAC TCTGTCGCTG 
CATTATTTTG AGGAACCGGA GTTGAGAGAT GGGTTTGTTC AGAATGTGCA CATTCCTATG 
GTTCGAGTGG ATCCAGAGAA TCGATGTGCT GTCATGCTGG TGTACGGCAC TTGCCTGGTG 
GTTCTGCCTT TCAGGAAGGA CACGCTTGCT GACGAACAGG AGGGGATTGT GGGAGAGGGG 
CAGAAATCTA GTTTCCTACC CAGCTACATC ATCGATGTTC GTGAACTGGA TGAGAAACTC 
CTAAACATCA TCGATATGAA GTTTCTCCAT GGCTACTATG AGCCCACACT GCTTATTCTG 
TTCGAGCCCA ATCAAACATG GCCAGGGCGT GTGGCGGTGC GTCAGGACAC TTGCAGTATT 
GTGGCGATCT CTCTAAACAT CATGCAGAAG GTTCATCCAG TCATCTGGTC TCTGAGTAAC 
CTGCCCTTTG ACTGCAATCA GGTCATGGCT GTCCCCAAAC CCATCGGTGG AGTTGTGGTG 
TTTGCTGTGA ATTCGTTGCT GTATCTTAAT CAGAGTGTTC CTCCGTTTGG AGTGTCTCTC 
AACAGTCTGA CTAACGGAAC CACAGCTTTC CCTTTGAGAC CTCAGGAGGA AGTAAAGATC 
ACCCTGGATT GTTCTCAAGC CTCCTTCATC ACCTCTGACA AGATGGTCAT CTCACTAAAA 
GGAGGAGAAA TTTATGTGTT GACGCTCATC ACTGATGGCA TGAGAAGTGT TCGTGCGTTT 
CACTTTGATA AGGCTGCTGC CAGTGTCCTG ACTACCTGTA TGATGACTAT GGAGCCAGGC 
TATCTGTTTT TGGGCTCTCG CCTGGGAAAC TCACTGCTGC TCAGGTACAC TGAGAAACTC 
CAGGAAACAC CCATGGAGGA GGGCAAAGAG AATGAGGAGA AGGAGAAACA GCCTCCAAAT 
AAGAAAAAAC GTGTGGATTC CAACTGGGCA GGGTGTCCAG GAAAGGGCAA TCTACCTGAT 
GAGCTGGATG AAATTGAGGT GTATGGGAGT GAAGCTCAGT CAGGCACTCA GCTGGCCACA 
TACTCATTTG AGGTTTGTGA CAGCATACTA AACATTGGAC CCTGTGCCAG TGCCTCCATG 
GGAGAACCAG CTTTTCTGTC TGAAGAGTTT CAAACCAACC CTGAGCCAGA TCTGGAGGTG 
GTGGTGTGTT CAGGATATGG CAAGAATGGA GCACTGTCTG TATTACAGAA AAGCATCAGA 
CCACAGGTGG TGACGACGTT CGAACTTCCT GGATGCCATG ACATGTGGAC AGTCATATAC 
TGTGAGGAGA AACCCGAAAA GCCTTCAGCT GAGGGTGATG GAGAGAGTCC TGAGGAGGAG 
AAGCGCGAGC CCACAATAGA GGATGACAAG AAGAAACACG GCTTCCTCAT CCTCAGCAGA 
GAAGACTCCA CCATGATTCT CCAGACGGGT CAAGAGATCA TGGAACTGGA CACCAGTGGA 
TTCGCCACAC AGGGTCCTAC AGTTTACGCG GGCAACATCG GAGACAATAA ATACATCATC 
CAGGTGTCCC CCATGGGCAT CAGACTGCTC GAGGGAGTTA ATCAGCTCCA CTTTATCCCT 
GTGGACCTGG GCTCTCCCAT AGTGCACTGC TCTGTGGCCG ATCCTTACGT GGTCATCATG 
ACGGCAGAGG GAGTGGTCAC TATGTTTGTC TTGAAGAATG ACTCGTATAT GGGAAAGAGT 
CACCGCCTGG CACTTCAGAA ACCACAGATA CACACGCAAT CACGTGTTAT TACGTTATGT 
GCATATCGCG ATGTCAGTGG TATGTTCACA ACTGAGAACA AGGTCAGCTT CCTGGCAAAA 
GAGGAAATTG CTATCAGAAC CAACTCAGAG ACAGAAACCA TCATCCAGGA CATCAGTAAT 
ACAGTGGATG ATGAAGAGGA GATGCTGTAC GGGGAGTCAA ACCCCCTGAC GAGCCCCAAC 
AAAGAAGAGT CCAGCCGTGG CTCTGCAGCA GCAAGCTCCG CTCACACAGG AAAAGAGAGC 
GGCTCCGGCA GACAGGAGCC CTCACACTGG TGCCTGCTGG TTAGAGAGAA TGGAGTCATG 
GAGATCTACC AGCTTCCAGA CTGGCGTTTG GTTTTCCTGG TGAAAAATTT CCCAGTCGGT 
CAGCGTGTTT TGGTTGACAG CTCTGCCAGC CAGTCAGCTA CTCAAGGGGA GCTTAAAAAA 
GAGGAAGTGA CGAGGCAAGG TGACATTCCA TTGGTCAAAG AGGTTGCTCT TGTGTCATTA 
GGCTACAACC ACAGCCGTCC CTACCTTCTG GCTCATGTTG AACAAGAGCT TCTCATCTAT 
GAGGCCTTTC CGTACGACCA GCAGCAAGCT CAAAGCAACC TGAAAGTGCG CTTCAAGAAG 
ATGCCTCATA ACATTAACTA CAGAGAGAAG AAGGTCAAAG TGAGGAAGGA CAAGAAGCCT 
GAGGGTCAGG GAGAGGACAC TCTCGGCGTC AAAGGTCGTG TGGCCAGATT CAGATACTTC 
CAGGACATCT CTGGATACTC AGGGGTGTTT ATCTGTGGTC CCTCTCCACA CTGGATGTTG 
GTGACCTCTC GCGGGGCGAT GCGCCTCCAC CCCATGACAA TAGATGGAGC CATTGAGTCT 
TTCTCTCCCT TTCACAATAT CAACTGCCCC AAAGGATTTC TCTACTTCAA CAAACAGGGT 
GAGCTGAGGA TCAGTGTGTT GCCAACATAC CTCTCTTATG ATGCTCCCTG GCCTGTGCGC 
AAGATCCCTC TTAGGTGCAC CGTCCACTAT GTATCATACC ATGTGGAATC CAAGGTGTAT 
GCAGTGTGCA CCAGTGTTAA AGAGCCGTGC ACGCGCATCC CCAGAATGAC TGGAGAAGAG 
AAGGAATTTG AGACCATTGA AAGAGACGAG CGATATATTC ACCCTCAACA GGACAAGTTC 
TCAATTCAGC TCATCTCTCC AGTGAGCTGG GAGGCGATTC CAAACACACG CGTTGACCTT 
GAGGAGTGGG AGCATGTGAC GTGCATGAAG ACGGTGGCTC TGAAGAGTCA GGAGACAGTG 
TCAGGACTAA AGGGATACGT GGCTCTTGGG ACGTGTCTCA TGCAGGGAGA AGAGGTCACC 
TGTAGAGGCA GAATCCTGAT TCTGGATGTG ATAGAGGTGG TCCCAGAGCC CGGACAGCCC 
CTCACCAAGA ACAAGTTTAA AGTGCTGTAT GAAAAGGAGC AGAAAGGTCC CGTCACTGCT 
CTTTGTCACT GCAGTGGATT CCTGGTGTCT GCTATTGGAC AGAAGATCTT CCTGTGGAGT 
CTGAAGGATA ATGATCTGAC GGGAATGGCA TTCATTGACA CACAGCTCTA CATTCATCAG 
ATGTACAGCA TCAAGAACTT CATCCTCGCA GCAGATGTGA TGAAGAGCAT CTCTCTGCTG 
CGCTACCAGC CGGAGAGCAA GACGCTTTCA CTCGTCAGCA GGGATGCAAA GCCCCTTGAG 
GTTTACAGCA TAGAGTTTAT GGTAGACAAT AACCAGCTTG GTTTTTTGGT TTCAGACAGA 
GACAAAAACC TTATGGTCTA CATGTACCTG CCTGAAGCCA AAGAGAGTTT TGGAGGCATG 
CGTTTGTTGC GGAGAGCAGA TTTTAACGTG GGATCGCATG TGAACGCCTT CTGGAGGATG 
CCATGCCGCG GGACACTGGA CACAGCCAAC AAGAAGGCCC TCACCTGGGA CAACAAACAC 
ATCACCTGGT TTGCTACGCT GGATGGTGGT GTCGGTCTGC TTTTACCAAT GCAGGAGAAG 
ACGTACCGCA GACTGCTGAT GTTGCAGAAC GCTCTCACCA CCATGCTGCC ACATCATGCC 
GGGCTCAATC CAAAGGCTTT CAGGATGCTG CACTGTGACC GGCGAACGCT CCAGAATGCT 
GTCAAGAACA TCCTGGATGG AGAGCTGCTC AATAAATACC TGTATCTCAG TACCATGGAG 
AGAAGTGAAC TGGCCAAGAA GATCGGCACG ACCCCTGACA TTATCTTGGA TGATCTTCTG 
GAGATTGAAA GAGTCACTGC TCACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001108153.2
CDS	169..4518
Translation	MYAVYRQAHPPTAVEFAVYCNFISSQEKNLVVAGTSQLYVYRIIYDVESTSKSEKSSDGKSRKEKLEQVASFSLFGNVMSMASVQLVGTNRDALLLSFKDAKLSVVEYDPGTHDLKTLSLHYFEEPELRDGFVQNVHIPMVRVDPENRCAVMLVYGTCLVVLPFRKDTLADEQEGIVGEGQKSSFLPSYIIDVRELDEKLLNIIDMKFLHGYYEPTLLILFEPNQTWPGRVAVRQDTCSIVAISLNIMQKVHPVIWSLSNLPFDCNQVMAVPKPIGGVVVFAVNSLLYLNQSVPPFGVSLNSLTNGTTAFPLRPQEEVKITLDCSQASFITSDKMVISLKGGEIYVLTLITDGMRSVRAFHFDKAAASVLTTCMMTMEPGYLFLGSRLGNSLLLRYTEKLQETPMEEGKENEEKEKQPPNKKKRVDSNWAGCPGKGNLPDELDEIEVYGSEAQSGTQLATYSFEVCDSILNIGPCASASMGEPAFLSEEFQTNPEPDLEVVVCSGYGKNGALSVLQKSIRPQVVTTFELPGCHDMWTVIYCEEKPEKPSAEGDGESPEEEKREPTIEDDKKKHGFLILSREDSTMILQTGQEIMELDTSGFATQGPTVYAGNIGDNKYIIQVSPMGIRLLEGVNQLHFIPVDLGSPIVHCSVADPYVVIMTAEGVVTMFVLKNDSYMGKSHRLALQKPQIHTQSRVITLCAYRDVSGMFTTENKVSFLAKEEIAIRTNSETETIIQDISNTVDDEEEMLYGESNPLTSPNKEESSRGSAAASSAHTGKESGSGRQEPSHWCLLVRENGVMEIYQLPDWRLVFLVKNFPVGQRVLVDSSASQSATQGELKKEEVTRQGDIPLVKEVALVSLGYNHSRPYLLAHVEQELLIYEAFPYDQQQAQSNLKVRFKKMPHNINYREKKVKVRKDKKPEGQGEDTLGVKGRVARFRYFQDISGYSGVFICGPSPHWMLVTSRGAMRLHPMTIDGAIESFSPFHNINCPKGFLYFNKQGELRISVLPTYLSYDAPWPVRKIPLRCTVHYVSYHVESKVYAVCTSVKEPCTRIPRMTGEEKEFETIERDERYIHPQQDKFSIQLISPVSWEAIPNTRVDLEEWEHVTCMKTVALKSQETVSGLKGYVALGTCLMQGEEVTCRGRILILDVIEVVPEPGQPLTKNKFKVLYEKEQKGPVTALCHCSGFLVSAIGQKIFLWSLKDNDLTGMAFIDTQLYIHQMYSIKNFILAADVMKSISLLRYQPESKTLSLVSRDAKPLEVYSIEFMVDNNQLGFLVSDRDKNLMVYMYLPEAKESFGGMRLLRRADFNVGSHVNAFWRMPCRGTLDTANKKALTWDNKHITWFATLDGGVGLLLPMQEKTYRRLLMLQNALTTMLPHHAGLNPKAFRMLHCDRRTLQNAVKNILDGELLNKYLYLSTMERSELAKKIGTTPDIILDDLLEIERVTAHF

Target ORF information:

RefSeq Version	NM_001114681.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cleavage and polyadenylation specific factor 1 (cpsf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001114681.2

ORF Insert Sequence:

ATGTACGCGG TGTACCGTCA GGCTCACCCG CCCACCGCAG TGGAGTTTGC CGTTTACTGC 
AACTTCATCT CCAGCCAAGA GAAAAACTTG GTGGTGGCAG GAACATCTCA GCTGTACGTC 
TACAGGATCA TTTATGATGT GGAGAGCACT TCCAAGTCTG AAAAGTCATC AGATGGCAAG 
AGTCGTAAAG AGAAACTGGA GCAGGTGGCA TCTTTCTCTC TCTTTGGAAA TGTGATGTCA 
ATGGCCAGCG TTCAGCTGGT GGGCACCAAC AGAGACGCCT TGCTTCTCAG CTTCAAAGAT 
GCCAAGCTGT CAGTAGTGGA GTATGACCCT GGCACGCATG ATCTCAAGAC TCTGTCGCTG 
CATTATTTTG AGGAACCGGA GTTGAGAGAT GGGTTTGTTC AGAATGTGCA CATTCCTATG 
GTTCGAGTGG ATCCAGAGAA TCGATGTGCT GTCATGCTGG TGTACGGCAC TTGCCTGGTG 
GTTCTGCCTT TCAGGAAGGA CACGCTTGCT GACGAACAGG AGGGGATTGT GGGAGAGGGG 
CAGAAATCTA GTTTCCTACC CAGCTACATC ATCGATGTTC GTGAACTGGA TGAGAAACTC 
CTAAACATCA TCGATATGAA GTTTCTCCAT GGCTACTATG AGCCCACACT GCTTATTCTG 
TTCGAGCCCA ATCAAACATG GCCAGGGCGT GTGGCGGTGC GTCAGGACAC TTGCAGTATT 
GTGGCGATCT CTCTAAACAT CATGCAGAAG GTTCATCCAG TCATCTGGTC TCTGAGTAAC 
CTGCCCTTTG ACTGCAATCA GGTCATGGCT GTCCCCAAAC CCATCGGTGG AGTTGTGGTG 
TTTGCTGTGA ATTCGTTGCT GTATCTTAAT CAGAGTGTTC CTCCGTTTGG AGTGTCTCTC 
AACAGTCTGA CTAACGGAAC CACAGCTTTC CCTTTGAGAC CTCAGGAGGA AGTAAAGATC 
ACCCTGGATT GTTCTCAAGC CTCCTTCATC ACCTCTGACA AGATGGTCAT CTCACTAAAA 
GGAGGAGAAA TTTATGTGTT GACGCTCATC ACTGATGGCA TGAGAAGTGT TCGTGCGTTT 
CACTTTGATA AGGCTGCTGC CAGTGTCCTG ACTACCTGTA TGATGACTAT GGAGCCAGGC 
TATCTGTTTT TGGGCTCTCG CCTGGGAAAC TCACTGCTGC TCAGGTACAC TGAGAAACTC 
CAGGAAACAC CCATGGAGGA GGGCAAAGAG AATGAGGAGA AGGAGAAACA GCCTCCAAAT 
AAGAAAAAAC GTGTGGATTC CAACTGGGCA GGGTGTCCAG GAAAGGGCAA TCTACCTGAT 
GAGCTGGATG AAATTGAGGT GTATGGGAGT GAAGCTCAGT CAGGCACTCA GCTGGCCACA 
TACTCATTTG AGGTTTGTGA CAGCATACTA AACATTGGAC CCTGTGCCAG TGCCTCCATG 
GGAGAACCAG CTTTTCTGTC TGAAGAGTTT CAAACCAACC CTGAGCCAGA TCTGGAGGTG 
GTGGTGTGTT CAGGATATGG CAAGAATGGA GCACTGTCTG TATTACAGAA AAGCATCAGA 
CCACAGGTGG TGACGACGTT CGAACTTCCT GGATGCCATG ACATGTGGAC AGTCATATAC 
TGTGAGGAGA AACCCGAAAA GCCTTCAGCT GAGGGTGATG GAGAGAGTCC TGAGGAGGAG 
AAGCGCGAGC CCACAATAGA GGATGACAAG AAGAAACACG GCTTCCTCAT CCTCAGCAGA 
GAAGACTCCA CCATGATTCT CCAGACGGGT CAAGAGATCA TGGAACTGGA CACCAGTGGA 
TTCGCCACAC AGGGTCCTAC AGTTTACGCG GGCAACATCG GAGACAATAA ATACATCATC 
CAGGTGTCCC CCATGGGCAT CAGACTGCTC GAGGGAGTTA ATCAGCTCCA CTTTATCCCT 
GTGGACCTGG GCTCTCCCAT AGTGCACTGC TCTGTGGCCG ATCCTTACGT GGTCATCATG 
ACGGCAGAGG GAGTGGTCAC TATGTTTGTC TTGAAGAATG ACTCGTATAT GGGAAAGAGT 
CACCGCCTGG CACTTCAGAA ACCACAGATA CACACGCAAT CACGTGTTAT TACGTTATGT 
GCATATCGCG ATGTCAGTGG TATGTTCACA ACTGAGAACA AGGTCAGCTT CCTGGCAAAA 
GAGGAAATTG CTATCAGAAC CAACTCAGAG ACAGAAACCA TCATCCAGGA CATCAGTAAT 
ACAGTGGATG ATGAAGAGGA GATGCTGTAC GGGGAGTCAA ACCCCCTGAC GAGCCCCAAC 
AAAGAAGAGT CCAGCCGTGG CTCTGCAGCA GCAAGCTCCG CTCACACAGG AAAAGAGAGC 
GGCTCCGGCA GACAGGAGCC CTCACACTGG TGCCTGCTGG TTAGAGAGAA TGGAGTCATG 
GAGATCTACC AGCTTCCAGA CTGGCGTTTG GTTTTCCTGG TGAAAAATTT CCCAGTCGGT 
CAGCGTGTTT TGGTTGACAG CTCTGCCAGC CAGTCAGCTA CTCAAGGGGA GCTTAAAAAA 
GAGGAAGTGA CGAGGCAAGG TGACATTCCA TTGGTCAAAG AGGTTGCTCT TGTGTCATTA 
GGCTACAACC ACAGCCGTCC CTACCTTCTG GCTCATGTTG AACAAGAGCT TCTCATCTAT 
GAGGCCTTTC CGTACGACCA GCAGCAAGCT CAAAGCAACC TGAAAGTGCG CTTCAAGAAG 
ATGCCTCATA ACATTAACTA CAGAGAGAAG AAGGTCAAAG TGAGGAAGGA CAAGAAGCCT 
GAGGGTCAGG GAGAGGACAC TCTCGGCGTC AAAGGTCGTG TGGCCAGATT CAGATACTTC 
CAGGACATCT CTGGATACTC AGGGGTGTTT ATCTGTGGTC CCTCTCCACA CTGGATGTTG 
GTGACCTCTC GCGGGGCGAT GCGCCTCCAC CCCATGACAA TAGATGGAGC CATTGAGTCT 
TTCTCTCCCT TTCACAATAT CAACTGCCCC AAAGGATTTC TCTACTTCAA CAAACAGGGT 
GAGCTGAGGA TCAGTGTGTT GCCAACATAC CTCTCTTATG ATGCTCCCTG GCCTGTGCGC 
AAGATCCCTC TTAGGTGCAC CGTCCACTAT GTATCATACC ATGTGGAATC CAAGGTGTAT 
GCAGTGTGCA CCAGTGTTAA AGAGCCGTGC ACGCGCATCC CCAGAATGAC TGGAGAAGAG 
AAGGAATTTG AGACCATTGA AAGAGACGAG CGATATATTC ACCCTCAACA GGACAAGTTC 
TCAATTCAGC TCATCTCTCC AGTGAGCTGG GAGGCGATTC CAAACACACG CGTTGACCTT 
GAGGAGTGGG AGCATGTGAC GTGCATGAAG ACGGTGGCTC TGAAGAGTCA GGAGACAGTG 
TCAGGACTAA AGGGATACGT GGCTCTTGGG ACGTGTCTCA TGCAGGGAGA AGAGGTCACC 
TGTAGAGGCA GAATCCTGAT TCTGGATGTG ATAGAGGTGG TCCCAGAGCC CGGACAGCCC 
CTCACCAAGA ACAAGTTTAA AGTGCTGTAT GAAAAGGAGC AGAAAGGTCC CGTCACTGCT 
CTTTGTCACT GCAGTGGATT CCTGGTGTCT GCTATTGGAC AGAAGATCTT CCTGTGGAGT 
CTGAAGGATA ATGATCTGAC GGGAATGGCA TTCATTGACA CACAGCTCTA CATTCATCAG 
ATGTACAGCA TCAAGAACTT CATCCTCGCA GCAGATGTGA TGAAGAGCAT CTCTCTGCTG 
CGCTACCAGC CGGAGAGCAA GACGCTTTCA CTCGTCAGCA GGGATGCAAA GCCCCTTGAG 
GTTTACAGCA TAGAGTTTAT GGTAGACAAT AACCAGCTTG GTTTTTTGGT TTCAGACAGA 
GACAAAAACC TTATGGTCTA CATGTACCTG CCTGAAGCCA AAGAGAGTTT TGGAGGCATG 
CGTTTGTTGC GGAGAGCAGA TTTTAACGTG GGATCGCATG TGAACGCCTT CTGGAGGATG 
CCATGCCGCG GGACACTGGA CACAGCCAAC AAGAAGGCCC TCACCTGGGA CAACAAACAC 
ATCACCTGGT TTGCTACGCT GGATGGTGGT GTCGGTCTGC TTTTACCAAT GCAGGAGAAG 
ACGTACCGCA GACTGCTGAT GTTGCAGAAC GCTCTCACCA CCATGCTGCC ACATCATGCC 
GGGCTCAATC CAAAGGCTTT CAGGATGCTG CACTGTGACC GGCGAACGCT CCAGAATGCT 
GTCAAGAACA TCCTGGATGG AGAGCTGCTC AATAAATACC TGTATCTCAG TACCATGGAG 
AGAAGTGAAC TGGCCAAGAA GATCGGCACG ACCCCTGACA TTATCTTGGA TGATCTTCTG 
GAGATTGAAA GAGTCACTGC TCACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cpsf1 ( XM_005159584.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159641.1 Danio rerio cleavage and polyadenylation specific factor 1 (cpsf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa23997
Clone ID Related Accession (Same CDS sequence)	XM_005159584.3 , XM_005159584.4
Accession Version	XM_005159584.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4347bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	cleavage and polyadenylation specificity factor subunit 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159584.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACGCGG TGTACCGTCA GGCTCACCCG CCCACCGCAG TGGAGTTTGC CGTTTACTGC 
AACTTCATCT CCAGCCAAGA GAAAAACTTG GTGGTGGCAG GAACATCTCA GCTGTACGTC 
TACAGGATCA TTTATGATGT GGAGAGCACT TCCAAGTCTG AAAAGTCATC AGATGGCAAG 
AGTCGTAAAG AGAAACTGGA GCAGGTGGCA TCTTTCTCTC TCTTTGGAAA TGTGATGTCA 
ATGGCCAGCG TTCAGCTGGT GGGCACCAAC AGAGACGCCT TGCTTCTCAG CTTCAAAGAT 
GCCAAGCTGT CAGTAGTGGA GTATGACCCT GGCACGCATG ATCTCAAGAC TCTGTCGCTG 
CATTATTTTG AGGAACCGGA GTTGAGAGAT GGGTTTGTTC AGAATGTGCA CATTCCTATG 
GTTCGAGTGG ATCCAGAGAA TCGATGTGCT GTCATGCTGG TGTACGGCAC TTGCCTGGTG 
GTTCTGCCTT TCAGGAAGGA CACGCTTGCT GACGAACAGG AGGGGATTGT GGGAGAGGGG 
CAGAAATCTA GTTTCCTACC CAGCTACATC ATCGATGTTC GTGAACTGGA TGAGAAACTC 
CTAAACATCA TCGATATGAA GTTTCTCCAT GGCTACTATG AGCCCACACT GCTTATTCTG 
TTCGAGCCCA ATCAAACATG GCCAGGGCGT GTGGCGGTGC GTCAGGACAC TTGCAGTATT 
GTGGCGATCT CTCTAAACAT CATGCAGAAG GTTCATCCAG TCATCTGGTC TCTGAGTAAC 
CTGCCCTTTG ACTGCAATCA GGTCATGGCT GTCCCCAAAC CCATCGGTGG AGTTGTGGTG 
TTTGCTGTGA ATTCGTTGCT GTATCTTAAT CAGAGTGTTC CTCCGTTTGG AGTGTCTCTC 
AACAGTCTGA CTAACGGAAC CACAGCTTTC CCTTTGAGAC CTCAGGAGGA AGTAAAGATC 
ACCCTGGATT GTTCTCAAGC CTCCTTCATC ACCTCTGACA AGATGGTCAT CTCACTAAAA 
GGAGGAGAAA TTTATGTGTT GACGCTCATC ACTGATGGCA TGAGAAGTGT TCGTGCGTTT 
CACTTTGATA AGGCTGCTGC CAGTGTCCTG ACTACCTGTA TGATGACTAT GGAGCCAGGC 
TATCTGTTTT TGGGCTCTCG CCTGGGAAAC TCACTGCTGC TCAGGTACAC TGAGAAACTC 
CAGGAAACAC CCATGGAGGA GGGCAAAGAG AATGAGGAGA AGGAGAAACA GGAAGAGCCT 
CCAAATAAGA AAAAACGTGT GGATTCCAAC TGGGCAGGAA AGGGCAATCT ACCTGATGAG 
CTGGATGAAA TTGAGGTGTA TGGGAGTGAA GCTCAGTCAG GCACTCAGCT GGCCACATAC 
TCATTTGAGG TTTGTGACAG CATACTAAAC ATTGGACCCT GTGCCAGTGC CTCCATGGGA 
GAACCAGCTT TTCTGTCTGA AGAGTTTCAA ACCAACCCTG AGCCAGATCT GGAGGTGGTG 
GTGTGTTCAG GATATGGCAA GAATGGAGCA CTGTCTGTAT TACAGAAAAG CATCAGACCA 
CAGGTGGTGA CGACGTTCGA ACTTCCTGGA TGCCATGACA TGTGGACAGT CATATACTGT 
GAGGAGAAAC CCGAAAAGCC TTCAGCTGAG GGTGATGGAG AGAGTCCTGA GGAGGAGAAG 
CGCGAGCCCA CAATAGAGGA TGACAAGAAG AAACACGGCT TCCTCATCCT CAGCAGAGAA 
GACTCCACCA TGATTCTCCA GACGGGTCAA GAGATCATGG AACTGGACAC CAGTGGATTC 
GCCACACAGG GTCCTACAGT TTACGCGGGC AACATCGGAG ACAATAAATA CATCATCCAG 
GTGTCCCCCA TGGGCATCAG ACTGCTCGAG GGAGTTAATC AGCTCCACTT TATCCCTGTG 
GACCTGGGCT CTCCCATAGT GCACTGCTCT GTGGCCGATC CTTACGTGGT CATCATGACG 
GCAGAGGGAG TGGTCACTAT GTTTGTCTTG AAGAATGACT CGTATATGGG AAAGAGTCAC 
CGCCTGGCAC TTCAGAAACC ACAGATACAC ACGCAATCAC GTGTTATTAC GTTATGTGCA 
TATCGCGATG TCAGTGGTAT GTTCACAACT GAGAACAAGG TCAGCTTCCT GGCAAAAGAG 
GAAATTGCTA TCAGAACCAA CTCAGAGACA GAAACCATCA TCCAGGACAT CAGTAATACA 
GTGGATGATG AAGAGGAGAT GCTGTACGGG GAGTCAAACC CCCTGACGAG CCCCAACAAA 
GAAGAGTCCA GCCGTGGCTC TGCAGCAGCA AGCTCCGCTC ACACAGGAAA AGAGAGCGGC 
TCCGGCAGAC AGGAGCCCTC ACACTGGTGC CTGCTGGTTA GAGAGAATGG AGTCATGGAG 
ATCTACCAGC TTCCAGACTG GCGTTTGGTT TTCCTGGTGA AAAATTTCCC AGTCGGTCAG 
CGTGTTTTGG TTGACAGCTC TGCCAGCCAG TCAGCTACTC AAGGGGAGCT TAAAAAAGAG 
GAAGTGACGA GGCAAGGTGA CATTCCATTG GTCAAAGAGG TTGCTCTTGT GTCATTAGGC 
TACAACCACA GCCGTCCCTA CCTTCTGGCT CATGTTGAAC AAGAGCTTCT CATCTATGAG 
GCCTTTCCGT ACGACCAGCA GCAAGCTCAA AGCAACCTGA AAGTGCGCTT CAAGAAGATG 
CCTCATAACA TTAACTACAG AGAGAAGAAG GTCAAAGTGA GGAAGGACAA GAAGCCTGAG 
GGTCAGGGAG AGGACACTCT CGGCGTCAAA GGTCGTGTGG CCAGATTCAG ATACTTCCAG 
GACATCTCTG GATACTCAGG GGTGTTTATC TGTGGTCCCT CTCCACACTG GATGTTGGTG 
ACCTCTCGCG GGGCGATGCG CCTCCACCCC ATGACAATAG ATGGAGCCAT TGAGTCTTTC 
TCTCCCTTTC ACAATATCAA CTGCCCCAAA GGATTTCTCT ACTTCAACAA ACAGGGTGAG 
CTGAGGATCA GTGTGTTGCC AACATACCTC TCTTATGATG CTCCCTGGCC TGTGCGCAAG 
ATCCCTCTTA GGTGCACCGT CCACTATGTA TCATACCATG TGGAATCCAA GGTGTATGCA 
GTGTGCACCA GTGTTAAAGA GCCGTGCACG CGCATCCCCA GAATGACTGG AGAAGAGAAG 
GAATTTGAGA CCATTGAAAG AGACGAGCGA TATATTCACC CTCAACAGGA CAAGTTCTCA 
ATTCAGCTCA TCTCTCCAGT GAGCTGGGAG GCGATTCCAA ACACACGCGT TGACCTTGAG 
GAGTGGGAGC ATGTGACGTG CATGAAGACG GTGGCTCTGA AGAGTCAGGA GACAGTGTCA 
GGACTAAAGG GATACGTGGC TCTTGGGACG TGTCTCATGC AGGGAGAAGA GGTCACCTGT 
AGAGGCAGAA TCCTGATTCT GGATGTGATA GAGGTGGTCC CAGAGCCCGG ACAGCCCCTC 
ACCAAGAACA AGTTTAAAGT GCTGTATGAA AAGGAGCAGA AAGGTCCCGT CACTGCTCTT 
TGTCACTGCA GTGGATTCCT GGTGTCTGCT ATTGGACAGA AGATCTTCCT GTGGAGTCTG 
AAGGATAATG ATCTGACGGG AATGGCATTC ATTGACACAC AGCTCTACAT TCATCAGATG 
TACAGCATCA AGAACTTCAT CCTCGCAGCA GATGTGATGA AGAGCATCTC TCTGCTGCGC 
TACCAGCCGG AGAGCAAGAC GCTTTCACTC GTCAGCAGGG ATGCAAAGCC CCTTGAGGTT 
TACAGCATAG AGTTTATGGT AGACAATAAC CAGCTTGGTT TTTTGGTTTC AGACAGAGAC 
AAAAACCTTA TGGTCTACAT GTACCTGCCT GAAGCCAAAG AGAGTTTTGG AGGCATGCGT 
TTGTTGCGGA GAGCAGATTT TAACGTGGGA TCGCATGTGA ACGCCTTCTG GAGGATGCCA 
TGCCGCGGGA CACTGGACAC AGCCAACAAG AAGGCCCTCA CCTGGGACAA CAAACACATC 
ACCTGGTTTG CTACGCTGGA TGGTGGTGTC GGTCTGCTTT TACCAATGCA GGAGAAGACG 
TACCGCAGAC TGCTGATGTT GCAGAACGCT CTCACCACCA TGCTGCCACA TCATGCCGGG 
CTCAATCCAA AGGCTTTCAG GATGCTGCAC TGTGACCGGC GAACGCTCCA GAATGCTGTC 
AAGAACATCC TGGATGGAGA GCTGCTCAAT AAATACCTGT ATCTCAGTAC CATGGAGAGA 
AGTGAACTGG CCAAGAAGAT CGGCACGACC CCTGACATTA TCTTGGATGA TCTTCTGGAG 
ATTGAAAGAG TCACTGCTCA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159641.1
CDS	171..4517
Translation	MYAVYRQAHPPTAVEFAVYCNFISSQEKNLVVAGTSQLYVYRIIYDVESTSKSEKSSDGKSRKEKLEQVASFSLFGNVMSMASVQLVGTNRDALLLSFKDAKLSVVEYDPGTHDLKTLSLHYFEEPELRDGFVQNVHIPMVRVDPENRCAVMLVYGTCLVVLPFRKDTLADEQEGIVGEGQKSSFLPSYIIDVRELDEKLLNIIDMKFLHGYYEPTLLILFEPNQTWPGRVAVRQDTCSIVAISLNIMQKVHPVIWSLSNLPFDCNQVMAVPKPIGGVVVFAVNSLLYLNQSVPPFGVSLNSLTNGTTAFPLRPQEEVKITLDCSQASFITSDKMVISLKGGEIYVLTLITDGMRSVRAFHFDKAAASVLTTCMMTMEPGYLFLGSRLGNSLLLRYTEKLQETPMEEGKENEEKEKQEEPPNKKKRVDSNWAGKGNLPDELDEIEVYGSEAQSGTQLATYSFEVCDSILNIGPCASASMGEPAFLSEEFQTNPEPDLEVVVCSGYGKNGALSVLQKSIRPQVVTTFELPGCHDMWTVIYCEEKPEKPSAEGDGESPEEEKREPTIEDDKKKHGFLILSREDSTMILQTGQEIMELDTSGFATQGPTVYAGNIGDNKYIIQVSPMGIRLLEGVNQLHFIPVDLGSPIVHCSVADPYVVIMTAEGVVTMFVLKNDSYMGKSHRLALQKPQIHTQSRVITLCAYRDVSGMFTTENKVSFLAKEEIAIRTNSETETIIQDISNTVDDEEEMLYGESNPLTSPNKEESSRGSAAASSAHTGKESGSGRQEPSHWCLLVRENGVMEIYQLPDWRLVFLVKNFPVGQRVLVDSSASQSATQGELKKEEVTRQGDIPLVKEVALVSLGYNHSRPYLLAHVEQELLIYEAFPYDQQQAQSNLKVRFKKMPHNINYREKKVKVRKDKKPEGQGEDTLGVKGRVARFRYFQDISGYSGVFICGPSPHWMLVTSRGAMRLHPMTIDGAIESFSPFHNINCPKGFLYFNKQGELRISVLPTYLSYDAPWPVRKIPLRCTVHYVSYHVESKVYAVCTSVKEPCTRIPRMTGEEKEFETIERDERYIHPQQDKFSIQLISPVSWEAIPNTRVDLEEWEHVTCMKTVALKSQETVSGLKGYVALGTCLMQGEEVTCRGRILILDVIEVVPEPGQPLTKNKFKVLYEKEQKGPVTALCHCSGFLVSAIGQKIFLWSLKDNDLTGMAFIDTQLYIHQMYSIKNFILAADVMKSISLLRYQPESKTLSLVSRDAKPLEVYSIEFMVDNNQLGFLVSDRDKNLMVYMYLPEAKESFGGMRLLRRADFNVGSHVNAFWRMPCRGTLDTANKKALTWDNKHITWFATLDGGVGLLLPMQEKTYRRLLMLQNALTTMLPHHAGLNPKAFRMLHCDRRTLQNAVKNILDGELLNKYLYLSTMERSELAKKIGTTPDIILDDLLEIERVTAHF

Target ORF information:

RefSeq Version	XM_005159584.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio cleavage and polyadenylation specific factor 1 (cpsf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159584.3

ORF Insert Sequence:

ATGTACGCGG TGTACCGTCA GGCTCACCCG CCCACCGCAG TGGAGTTTGC CGTTTACTGC 
AACTTCATCT CCAGCCAAGA GAAAAACTTG GTGGTGGCAG GAACATCTCA GCTGTACGTC 
TACAGGATCA TTTATGATGT GGAGAGCACT TCCAAGTCTG AAAAGTCATC AGATGGCAAG 
AGTCGTAAAG AGAAACTGGA GCAGGTGGCA TCTTTCTCTC TCTTTGGAAA TGTGATGTCA 
ATGGCCAGCG TTCAGCTGGT GGGCACCAAC AGAGACGCCT TGCTTCTCAG CTTCAAAGAT 
GCCAAGCTGT CAGTAGTGGA GTATGACCCT GGCACGCATG ATCTCAAGAC TCTGTCGCTG 
CATTATTTTG AGGAACCGGA GTTGAGAGAT GGGTTTGTTC AGAATGTGCA CATTCCTATG 
GTTCGAGTGG ATCCAGAGAA TCGATGTGCT GTCATGCTGG TGTACGGCAC TTGCCTGGTG 
GTTCTGCCTT TCAGGAAGGA CACGCTTGCT GACGAACAGG AGGGGATTGT GGGAGAGGGG 
CAGAAATCTA GTTTCCTACC CAGCTACATC ATCGATGTTC GTGAACTGGA TGAGAAACTC 
CTAAACATCA TCGATATGAA GTTTCTCCAT GGCTACTATG AGCCCACACT GCTTATTCTG 
TTCGAGCCCA ATCAAACATG GCCAGGGCGT GTGGCGGTGC GTCAGGACAC TTGCAGTATT 
GTGGCGATCT CTCTAAACAT CATGCAGAAG GTTCATCCAG TCATCTGGTC TCTGAGTAAC 
CTGCCCTTTG ACTGCAATCA GGTCATGGCT GTCCCCAAAC CCATCGGTGG AGTTGTGGTG 
TTTGCTGTGA ATTCGTTGCT GTATCTTAAT CAGAGTGTTC CTCCGTTTGG AGTGTCTCTC 
AACAGTCTGA CTAACGGAAC CACAGCTTTC CCTTTGAGAC CTCAGGAGGA AGTAAAGATC 
ACCCTGGATT GTTCTCAAGC CTCCTTCATC ACCTCTGACA AGATGGTCAT CTCACTAAAA 
GGAGGAGAAA TTTATGTGTT GACGCTCATC ACTGATGGCA TGAGAAGTGT TCGTGCGTTT 
CACTTTGATA AGGCTGCTGC CAGTGTCCTG ACTACCTGTA TGATGACTAT GGAGCCAGGC 
TATCTGTTTT TGGGCTCTCG CCTGGGAAAC TCACTGCTGC TCAGGTACAC TGAGAAACTC 
CAGGAAACAC CCATGGAGGA GGGCAAAGAG AATGAGGAGA AGGAGAAACA GGAAGAGCCT 
CCAAATAAGA AAAAACGTGT GGATTCCAAC TGGGCAGGAA AGGGCAATCT ACCTGATGAG 
CTGGATGAAA TTGAGGTGTA TGGGAGTGAA GCTCAGTCAG GCACTCAGCT GGCCACATAC 
TCATTTGAGG TTTGTGACAG CATACTAAAC ATTGGACCCT GTGCCAGTGC CTCCATGGGA 
GAACCAGCTT TTCTGTCTGA AGAGTTTCAA ACCAACCCTG AGCCAGATCT GGAGGTGGTG 
GTGTGTTCAG GATATGGCAA GAATGGAGCA CTGTCTGTAT TACAGAAAAG CATCAGACCA 
CAGGTGGTGA CGACGTTCGA ACTTCCTGGA TGCCATGACA TGTGGACAGT CATATACTGT 
GAGGAGAAAC CCGAAAAGCC TTCAGCTGAG GGTGATGGAG AGAGTCCTGA GGAGGAGAAG 
CGCGAGCCCA CAATAGAGGA TGACAAGAAG AAACACGGCT TCCTCATCCT CAGCAGAGAA 
GACTCCACCA TGATTCTCCA GACGGGTCAA GAGATCATGG AACTGGACAC CAGTGGATTC 
GCCACACAGG GTCCTACAGT TTACGCGGGC AACATCGGAG ACAATAAATA CATCATCCAG 
GTGTCCCCCA TGGGCATCAG ACTGCTCGAG GGAGTTAATC AGCTCCACTT TATCCCTGTG 
GACCTGGGCT CTCCCATAGT GCACTGCTCT GTGGCCGATC CTTACGTGGT CATCATGACG 
GCAGAGGGAG TGGTCACTAT GTTTGTCTTG AAGAATGACT CGTATATGGG AAAGAGTCAC 
CGCCTGGCAC TTCAGAAACC ACAGATACAC ACGCAATCAC GTGTTATTAC GTTATGTGCA 
TATCGCGATG TCAGTGGTAT GTTCACAACT GAGAACAAGG TCAGCTTCCT GGCAAAAGAG 
GAAATTGCTA TCAGAACCAA CTCAGAGACA GAAACCATCA TCCAGGACAT CAGTAATACA 
GTGGATGATG AAGAGGAGAT GCTGTACGGG GAGTCAAACC CCCTGACGAG CCCCAACAAA 
GAAGAGTCCA GCCGTGGCTC TGCAGCAGCA AGCTCCGCTC ACACAGGAAA AGAGAGCGGC 
TCCGGCAGAC AGGAGCCCTC ACACTGGTGC CTGCTGGTTA GAGAGAATGG AGTCATGGAG 
ATCTACCAGC TTCCAGACTG GCGTTTGGTT TTCCTGGTGA AAAATTTCCC AGTCGGTCAG 
CGTGTTTTGG TTGACAGCTC TGCCAGCCAG TCAGCTACTC AAGGGGAGCT TAAAAAAGAG 
GAAGTGACGA GGCAAGGTGA CATTCCATTG GTCAAAGAGG TTGCTCTTGT GTCATTAGGC 
TACAACCACA GCCGTCCCTA CCTTCTGGCT CATGTTGAAC AAGAGCTTCT CATCTATGAG 
GCCTTTCCGT ACGACCAGCA GCAAGCTCAA AGCAACCTGA AAGTGCGCTT CAAGAAGATG 
CCTCATAACA TTAACTACAG AGAGAAGAAG GTCAAAGTGA GGAAGGACAA GAAGCCTGAG 
GGTCAGGGAG AGGACACTCT CGGCGTCAAA GGTCGTGTGG CCAGATTCAG ATACTTCCAG 
GACATCTCTG GATACTCAGG GGTGTTTATC TGTGGTCCCT CTCCACACTG GATGTTGGTG 
ACCTCTCGCG GGGCGATGCG CCTCCACCCC ATGACAATAG ATGGAGCCAT TGAGTCTTTC 
TCTCCCTTTC ACAATATCAA CTGCCCCAAA GGATTTCTCT ACTTCAACAA ACAGGGTGAG 
CTGAGGATCA GTGTGTTGCC AACATACCTC TCTTATGATG CTCCCTGGCC TGTGCGCAAG 
ATCCCTCTTA GGTGCACCGT CCACTATGTA TCATACCATG TGGAATCCAA GGTGTATGCA 
GTGTGCACCA GTGTTAAAGA GCCGTGCACG CGCATCCCCA GAATGACTGG AGAAGAGAAG 
GAATTTGAGA CCATTGAAAG AGACGAGCGA TATATTCACC CTCAACAGGA CAAGTTCTCA 
ATTCAGCTCA TCTCTCCAGT GAGCTGGGAG GCGATTCCAA ACACACGCGT TGACCTTGAG 
GAGTGGGAGC ATGTGACGTG CATGAAGACG GTGGCTCTGA AGAGTCAGGA GACAGTGTCA 
GGACTAAAGG GATACGTGGC TCTTGGGACG TGTCTCATGC AGGGAGAAGA GGTCACCTGT 
AGAGGCAGAA TCCTGATTCT GGATGTGATA GAGGTGGTCC CAGAGCCCGG ACAGCCCCTC 
ACCAAGAACA AGTTTAAAGT GCTGTATGAA AAGGAGCAGA AAGGTCCCGT CACTGCTCTT 
TGTCACTGCA GTGGATTCCT GGTGTCTGCT ATTGGACAGA AGATCTTCCT GTGGAGTCTG 
AAGGATAATG ATCTGACGGG AATGGCATTC ATTGACACAC AGCTCTACAT TCATCAGATG 
TACAGCATCA AGAACTTCAT CCTCGCAGCA GATGTGATGA AGAGCATCTC TCTGCTGCGC 
TACCAGCCGG AGAGCAAGAC GCTTTCACTC GTCAGCAGGG ATGCAAAGCC CCTTGAGGTT 
TACAGCATAG AGTTTATGGT AGACAATAAC CAGCTTGGTT TTTTGGTTTC AGACAGAGAC 
AAAAACCTTA TGGTCTACAT GTACCTGCCT GAAGCCAAAG AGAGTTTTGG AGGCATGCGT 
TTGTTGCGGA GAGCAGATTT TAACGTGGGA TCGCATGTGA ACGCCTTCTG GAGGATGCCA 
TGCCGCGGGA CACTGGACAC AGCCAACAAG AAGGCCCTCA CCTGGGACAA CAAACACATC 
ACCTGGTTTG CTACGCTGGA TGGTGGTGTC GGTCTGCTTT TACCAATGCA GGAGAAGACG 
TACCGCAGAC TGCTGATGTT GCAGAACGCT CTCACCACCA TGCTGCCACA TCATGCCGGG 
CTCAATCCAA AGGCTTTCAG GATGCTGCAC TGTGACCGGC GAACGCTCCA GAATGCTGTC 
AAGAACATCC TGGATGGAGA GCTGCTCAAT AAATACCTGT ATCTCAGTAC CATGGAGAGA 
AGTGAACTGG CCAAGAAGAT CGGCACGACC CCTGACATTA TCTTGGATGA TCTTCTGGAG 
ATTGAAAGAG TCACTGCTCA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cpsf1 ( XM_005159584.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159641.1 Danio rerio cleavage and polyadenylation specific factor 1 (cpsf1), transcript variant X1, mRNA.

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CloneID	ODa23997
Clone ID Related Accession (Same CDS sequence)	XM_005159584.3 , XM_005159584.4
Accession Version	XM_005159584.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4347bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	cleavage and polyadenylation specificity factor subunit 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159584.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACGCGG TGTACCGTCA GGCTCACCCG CCCACCGCAG TGGAGTTTGC CGTTTACTGC 
AACTTCATCT CCAGCCAAGA GAAAAACTTG GTGGTGGCAG GAACATCTCA GCTGTACGTC 
TACAGGATCA TTTATGATGT GGAGAGCACT TCCAAGTCTG AAAAGTCATC AGATGGCAAG 
AGTCGTAAAG AGAAACTGGA GCAGGTGGCA TCTTTCTCTC TCTTTGGAAA TGTGATGTCA 
ATGGCCAGCG TTCAGCTGGT GGGCACCAAC AGAGACGCCT TGCTTCTCAG CTTCAAAGAT 
GCCAAGCTGT CAGTAGTGGA GTATGACCCT GGCACGCATG ATCTCAAGAC TCTGTCGCTG 
CATTATTTTG AGGAACCGGA GTTGAGAGAT GGGTTTGTTC AGAATGTGCA CATTCCTATG 
GTTCGAGTGG ATCCAGAGAA TCGATGTGCT GTCATGCTGG TGTACGGCAC TTGCCTGGTG 
GTTCTGCCTT TCAGGAAGGA CACGCTTGCT GACGAACAGG AGGGGATTGT GGGAGAGGGG 
CAGAAATCTA GTTTCCTACC CAGCTACATC ATCGATGTTC GTGAACTGGA TGAGAAACTC 
CTAAACATCA TCGATATGAA GTTTCTCCAT GGCTACTATG AGCCCACACT GCTTATTCTG 
TTCGAGCCCA ATCAAACATG GCCAGGGCGT GTGGCGGTGC GTCAGGACAC TTGCAGTATT 
GTGGCGATCT CTCTAAACAT CATGCAGAAG GTTCATCCAG TCATCTGGTC TCTGAGTAAC 
CTGCCCTTTG ACTGCAATCA GGTCATGGCT GTCCCCAAAC CCATCGGTGG AGTTGTGGTG 
TTTGCTGTGA ATTCGTTGCT GTATCTTAAT CAGAGTGTTC CTCCGTTTGG AGTGTCTCTC 
AACAGTCTGA CTAACGGAAC CACAGCTTTC CCTTTGAGAC CTCAGGAGGA AGTAAAGATC 
ACCCTGGATT GTTCTCAAGC CTCCTTCATC ACCTCTGACA AGATGGTCAT CTCACTAAAA 
GGAGGAGAAA TTTATGTGTT GACGCTCATC ACTGATGGCA TGAGAAGTGT TCGTGCGTTT 
CACTTTGATA AGGCTGCTGC CAGTGTCCTG ACTACCTGTA TGATGACTAT GGAGCCAGGC 
TATCTGTTTT TGGGCTCTCG CCTGGGAAAC TCACTGCTGC TCAGGTACAC TGAGAAACTC 
CAGGAAACAC CCATGGAGGA GGGCAAAGAG AATGAGGAGA AGGAGAAACA GGAAGAGCCT 
CCAAATAAGA AAAAACGTGT GGATTCCAAC TGGGCAGGAA AGGGCAATCT ACCTGATGAG 
CTGGATGAAA TTGAGGTGTA TGGGAGTGAA GCTCAGTCAG GCACTCAGCT GGCCACATAC 
TCATTTGAGG TTTGTGACAG CATACTAAAC ATTGGACCCT GTGCCAGTGC CTCCATGGGA 
GAACCAGCTT TTCTGTCTGA AGAGTTTCAA ACCAACCCTG AGCCAGATCT GGAGGTGGTG 
GTGTGTTCAG GATATGGCAA GAATGGAGCA CTGTCTGTAT TACAGAAAAG CATCAGACCA 
CAGGTGGTGA CGACGTTCGA ACTTCCTGGA TGCCATGACA TGTGGACAGT CATATACTGT 
GAGGAGAAAC CCGAAAAGCC TTCAGCTGAG GGTGATGGAG AGAGTCCTGA GGAGGAGAAG 
CGCGAGCCCA CAATAGAGGA TGACAAGAAG AAACACGGCT TCCTCATCCT CAGCAGAGAA 
GACTCCACCA TGATTCTCCA GACGGGTCAA GAGATCATGG AACTGGACAC CAGTGGATTC 
GCCACACAGG GTCCTACAGT TTACGCGGGC AACATCGGAG ACAATAAATA CATCATCCAG 
GTGTCCCCCA TGGGCATCAG ACTGCTCGAG GGAGTTAATC AGCTCCACTT TATCCCTGTG 
GACCTGGGCT CTCCCATAGT GCACTGCTCT GTGGCCGATC CTTACGTGGT CATCATGACG 
GCAGAGGGAG TGGTCACTAT GTTTGTCTTG AAGAATGACT CGTATATGGG AAAGAGTCAC 
CGCCTGGCAC TTCAGAAACC ACAGATACAC ACGCAATCAC GTGTTATTAC GTTATGTGCA 
TATCGCGATG TCAGTGGTAT GTTCACAACT GAGAACAAGG TCAGCTTCCT GGCAAAAGAG 
GAAATTGCTA TCAGAACCAA CTCAGAGACA GAAACCATCA TCCAGGACAT CAGTAATACA 
GTGGATGATG AAGAGGAGAT GCTGTACGGG GAGTCAAACC CCCTGACGAG CCCCAACAAA 
GAAGAGTCCA GCCGTGGCTC TGCAGCAGCA AGCTCCGCTC ACACAGGAAA AGAGAGCGGC 
TCCGGCAGAC AGGAGCCCTC ACACTGGTGC CTGCTGGTTA GAGAGAATGG AGTCATGGAG 
ATCTACCAGC TTCCAGACTG GCGTTTGGTT TTCCTGGTGA AAAATTTCCC AGTCGGTCAG 
CGTGTTTTGG TTGACAGCTC TGCCAGCCAG TCAGCTACTC AAGGGGAGCT TAAAAAAGAG 
GAAGTGACGA GGCAAGGTGA CATTCCATTG GTCAAAGAGG TTGCTCTTGT GTCATTAGGC 
TACAACCACA GCCGTCCCTA CCTTCTGGCT CATGTTGAAC AAGAGCTTCT CATCTATGAG 
GCCTTTCCGT ACGACCAGCA GCAAGCTCAA AGCAACCTGA AAGTGCGCTT CAAGAAGATG 
CCTCATAACA TTAACTACAG AGAGAAGAAG GTCAAAGTGA GGAAGGACAA GAAGCCTGAG 
GGTCAGGGAG AGGACACTCT CGGCGTCAAA GGTCGTGTGG CCAGATTCAG ATACTTCCAG 
GACATCTCTG GATACTCAGG GGTGTTTATC TGTGGTCCCT CTCCACACTG GATGTTGGTG 
ACCTCTCGCG GGGCGATGCG CCTCCACCCC ATGACAATAG ATGGAGCCAT TGAGTCTTTC 
TCTCCCTTTC ACAATATCAA CTGCCCCAAA GGATTTCTCT ACTTCAACAA ACAGGGTGAG 
CTGAGGATCA GTGTGTTGCC AACATACCTC TCTTATGATG CTCCCTGGCC TGTGCGCAAG 
ATCCCTCTTA GGTGCACCGT CCACTATGTA TCATACCATG TGGAATCCAA GGTGTATGCA 
GTGTGCACCA GTGTTAAAGA GCCGTGCACG CGCATCCCCA GAATGACTGG AGAAGAGAAG 
GAATTTGAGA CCATTGAAAG AGACGAGCGA TATATTCACC CTCAACAGGA CAAGTTCTCA 
ATTCAGCTCA TCTCTCCAGT GAGCTGGGAG GCGATTCCAA ACACACGCGT TGACCTTGAG 
GAGTGGGAGC ATGTGACGTG CATGAAGACG GTGGCTCTGA AGAGTCAGGA GACAGTGTCA 
GGACTAAAGG GATACGTGGC TCTTGGGACG TGTCTCATGC AGGGAGAAGA GGTCACCTGT 
AGAGGCAGAA TCCTGATTCT GGATGTGATA GAGGTGGTCC CAGAGCCCGG ACAGCCCCTC 
ACCAAGAACA AGTTTAAAGT GCTGTATGAA AAGGAGCAGA AAGGTCCCGT CACTGCTCTT 
TGTCACTGCA GTGGATTCCT GGTGTCTGCT ATTGGACAGA AGATCTTCCT GTGGAGTCTG 
AAGGATAATG ATCTGACGGG AATGGCATTC ATTGACACAC AGCTCTACAT TCATCAGATG 
TACAGCATCA AGAACTTCAT CCTCGCAGCA GATGTGATGA AGAGCATCTC TCTGCTGCGC 
TACCAGCCGG AGAGCAAGAC GCTTTCACTC GTCAGCAGGG ATGCAAAGCC CCTTGAGGTT 
TACAGCATAG AGTTTATGGT AGACAATAAC CAGCTTGGTT TTTTGGTTTC AGACAGAGAC 
AAAAACCTTA TGGTCTACAT GTACCTGCCT GAAGCCAAAG AGAGTTTTGG AGGCATGCGT 
TTGTTGCGGA GAGCAGATTT TAACGTGGGA TCGCATGTGA ACGCCTTCTG GAGGATGCCA 
TGCCGCGGGA CACTGGACAC AGCCAACAAG AAGGCCCTCA CCTGGGACAA CAAACACATC 
ACCTGGTTTG CTACGCTGGA TGGTGGTGTC GGTCTGCTTT TACCAATGCA GGAGAAGACG 
TACCGCAGAC TGCTGATGTT GCAGAACGCT CTCACCACCA TGCTGCCACA TCATGCCGGG 
CTCAATCCAA AGGCTTTCAG GATGCTGCAC TGTGACCGGC GAACGCTCCA GAATGCTGTC 
AAGAACATCC TGGATGGAGA GCTGCTCAAT AAATACCTGT ATCTCAGTAC CATGGAGAGA 
AGTGAACTGG CCAAGAAGAT CGGCACGACC CCTGACATTA TCTTGGATGA TCTTCTGGAG 
ATTGAAAGAG TCACTGCTCA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159641.1
CDS	190..4536
Translation	MYAVYRQAHPPTAVEFAVYCNFISSQEKNLVVAGTSQLYVYRIIYDVESTSKSEKSSDGKSRKEKLEQVASFSLFGNVMSMASVQLVGTNRDALLLSFKDAKLSVVEYDPGTHDLKTLSLHYFEEPELRDGFVQNVHIPMVRVDPENRCAVMLVYGTCLVVLPFRKDTLADEQEGIVGEGQKSSFLPSYIIDVRELDEKLLNIIDMKFLHGYYEPTLLILFEPNQTWPGRVAVRQDTCSIVAISLNIMQKVHPVIWSLSNLPFDCNQVMAVPKPIGGVVVFAVNSLLYLNQSVPPFGVSLNSLTNGTTAFPLRPQEEVKITLDCSQASFITSDKMVISLKGGEIYVLTLITDGMRSVRAFHFDKAAASVLTTCMMTMEPGYLFLGSRLGNSLLLRYTEKLQETPMEEGKENEEKEKQEEPPNKKKRVDSNWAGKGNLPDELDEIEVYGSEAQSGTQLATYSFEVCDSILNIGPCASASMGEPAFLSEEFQTNPEPDLEVVVCSGYGKNGALSVLQKSIRPQVVTTFELPGCHDMWTVIYCEEKPEKPSAEGDGESPEEEKREPTIEDDKKKHGFLILSREDSTMILQTGQEIMELDTSGFATQGPTVYAGNIGDNKYIIQVSPMGIRLLEGVNQLHFIPVDLGSPIVHCSVADPYVVIMTAEGVVTMFVLKNDSYMGKSHRLALQKPQIHTQSRVITLCAYRDVSGMFTTENKVSFLAKEEIAIRTNSETETIIQDISNTVDDEEEMLYGESNPLTSPNKEESSRGSAAASSAHTGKESGSGRQEPSHWCLLVRENGVMEIYQLPDWRLVFLVKNFPVGQRVLVDSSASQSATQGELKKEEVTRQGDIPLVKEVALVSLGYNHSRPYLLAHVEQELLIYEAFPYDQQQAQSNLKVRFKKMPHNINYREKKVKVRKDKKPEGQGEDTLGVKGRVARFRYFQDISGYSGVFICGPSPHWMLVTSRGAMRLHPMTIDGAIESFSPFHNINCPKGFLYFNKQGELRISVLPTYLSYDAPWPVRKIPLRCTVHYVSYHVESKVYAVCTSVKEPCTRIPRMTGEEKEFETIERDERYIHPQQDKFSIQLISPVSWEAIPNTRVDLEEWEHVTCMKTVALKSQETVSGLKGYVALGTCLMQGEEVTCRGRILILDVIEVVPEPGQPLTKNKFKVLYEKEQKGPVTALCHCSGFLVSAIGQKIFLWSLKDNDLTGMAFIDTQLYIHQMYSIKNFILAADVMKSISLLRYQPESKTLSLVSRDAKPLEVYSIEFMVDNNQLGFLVSDRDKNLMVYMYLPEAKESFGGMRLLRRADFNVGSHVNAFWRMPCRGTLDTANKKALTWDNKHITWFATLDGGVGLLLPMQEKTYRRLLMLQNALTTMLPHHAGLNPKAFRMLHCDRRTLQNAVKNILDGELLNKYLYLSTMERSELAKKIGTTPDIILDDLLEIERVTAHF

Target ORF information:

RefSeq Version	XM_005159584.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cleavage and polyadenylation specific factor 1 (cpsf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159584.4

ORF Insert Sequence:

ATGTACGCGG TGTACCGTCA GGCTCACCCG CCCACCGCAG TGGAGTTTGC CGTTTACTGC 
AACTTCATCT CCAGCCAAGA GAAAAACTTG GTGGTGGCAG GAACATCTCA GCTGTACGTC 
TACAGGATCA TTTATGATGT GGAGAGCACT TCCAAGTCTG AAAAGTCATC AGATGGCAAG 
AGTCGTAAAG AGAAACTGGA GCAGGTGGCA TCTTTCTCTC TCTTTGGAAA TGTGATGTCA 
ATGGCCAGCG TTCAGCTGGT GGGCACCAAC AGAGACGCCT TGCTTCTCAG CTTCAAAGAT 
GCCAAGCTGT CAGTAGTGGA GTATGACCCT GGCACGCATG ATCTCAAGAC TCTGTCGCTG 
CATTATTTTG AGGAACCGGA GTTGAGAGAT GGGTTTGTTC AGAATGTGCA CATTCCTATG 
GTTCGAGTGG ATCCAGAGAA TCGATGTGCT GTCATGCTGG TGTACGGCAC TTGCCTGGTG 
GTTCTGCCTT TCAGGAAGGA CACGCTTGCT GACGAACAGG AGGGGATTGT GGGAGAGGGG 
CAGAAATCTA GTTTCCTACC CAGCTACATC ATCGATGTTC GTGAACTGGA TGAGAAACTC 
CTAAACATCA TCGATATGAA GTTTCTCCAT GGCTACTATG AGCCCACACT GCTTATTCTG 
TTCGAGCCCA ATCAAACATG GCCAGGGCGT GTGGCGGTGC GTCAGGACAC TTGCAGTATT 
GTGGCGATCT CTCTAAACAT CATGCAGAAG GTTCATCCAG TCATCTGGTC TCTGAGTAAC 
CTGCCCTTTG ACTGCAATCA GGTCATGGCT GTCCCCAAAC CCATCGGTGG AGTTGTGGTG 
TTTGCTGTGA ATTCGTTGCT GTATCTTAAT CAGAGTGTTC CTCCGTTTGG AGTGTCTCTC 
AACAGTCTGA CTAACGGAAC CACAGCTTTC CCTTTGAGAC CTCAGGAGGA AGTAAAGATC 
ACCCTGGATT GTTCTCAAGC CTCCTTCATC ACCTCTGACA AGATGGTCAT CTCACTAAAA 
GGAGGAGAAA TTTATGTGTT GACGCTCATC ACTGATGGCA TGAGAAGTGT TCGTGCGTTT 
CACTTTGATA AGGCTGCTGC CAGTGTCCTG ACTACCTGTA TGATGACTAT GGAGCCAGGC 
TATCTGTTTT TGGGCTCTCG CCTGGGAAAC TCACTGCTGC TCAGGTACAC TGAGAAACTC 
CAGGAAACAC CCATGGAGGA GGGCAAAGAG AATGAGGAGA AGGAGAAACA GGAAGAGCCT 
CCAAATAAGA AAAAACGTGT GGATTCCAAC TGGGCAGGAA AGGGCAATCT ACCTGATGAG 
CTGGATGAAA TTGAGGTGTA TGGGAGTGAA GCTCAGTCAG GCACTCAGCT GGCCACATAC 
TCATTTGAGG TTTGTGACAG CATACTAAAC ATTGGACCCT GTGCCAGTGC CTCCATGGGA 
GAACCAGCTT TTCTGTCTGA AGAGTTTCAA ACCAACCCTG AGCCAGATCT GGAGGTGGTG 
GTGTGTTCAG GATATGGCAA GAATGGAGCA CTGTCTGTAT TACAGAAAAG CATCAGACCA 
CAGGTGGTGA CGACGTTCGA ACTTCCTGGA TGCCATGACA TGTGGACAGT CATATACTGT 
GAGGAGAAAC CCGAAAAGCC TTCAGCTGAG GGTGATGGAG AGAGTCCTGA GGAGGAGAAG 
CGCGAGCCCA CAATAGAGGA TGACAAGAAG AAACACGGCT TCCTCATCCT CAGCAGAGAA 
GACTCCACCA TGATTCTCCA GACGGGTCAA GAGATCATGG AACTGGACAC CAGTGGATTC 
GCCACACAGG GTCCTACAGT TTACGCGGGC AACATCGGAG ACAATAAATA CATCATCCAG 
GTGTCCCCCA TGGGCATCAG ACTGCTCGAG GGAGTTAATC AGCTCCACTT TATCCCTGTG 
GACCTGGGCT CTCCCATAGT GCACTGCTCT GTGGCCGATC CTTACGTGGT CATCATGACG 
GCAGAGGGAG TGGTCACTAT GTTTGTCTTG AAGAATGACT CGTATATGGG AAAGAGTCAC 
CGCCTGGCAC TTCAGAAACC ACAGATACAC ACGCAATCAC GTGTTATTAC GTTATGTGCA 
TATCGCGATG TCAGTGGTAT GTTCACAACT GAGAACAAGG TCAGCTTCCT GGCAAAAGAG 
GAAATTGCTA TCAGAACCAA CTCAGAGACA GAAACCATCA TCCAGGACAT CAGTAATACA 
GTGGATGATG AAGAGGAGAT GCTGTACGGG GAGTCAAACC CCCTGACGAG CCCCAACAAA 
GAAGAGTCCA GCCGTGGCTC TGCAGCAGCA AGCTCCGCTC ACACAGGAAA AGAGAGCGGC 
TCCGGCAGAC AGGAGCCCTC ACACTGGTGC CTGCTGGTTA GAGAGAATGG AGTCATGGAG 
ATCTACCAGC TTCCAGACTG GCGTTTGGTT TTCCTGGTGA AAAATTTCCC AGTCGGTCAG 
CGTGTTTTGG TTGACAGCTC TGCCAGCCAG TCAGCTACTC AAGGGGAGCT TAAAAAAGAG 
GAAGTGACGA GGCAAGGTGA CATTCCATTG GTCAAAGAGG TTGCTCTTGT GTCATTAGGC 
TACAACCACA GCCGTCCCTA CCTTCTGGCT CATGTTGAAC AAGAGCTTCT CATCTATGAG 
GCCTTTCCGT ACGACCAGCA GCAAGCTCAA AGCAACCTGA AAGTGCGCTT CAAGAAGATG 
CCTCATAACA TTAACTACAG AGAGAAGAAG GTCAAAGTGA GGAAGGACAA GAAGCCTGAG 
GGTCAGGGAG AGGACACTCT CGGCGTCAAA GGTCGTGTGG CCAGATTCAG ATACTTCCAG 
GACATCTCTG GATACTCAGG GGTGTTTATC TGTGGTCCCT CTCCACACTG GATGTTGGTG 
ACCTCTCGCG GGGCGATGCG CCTCCACCCC ATGACAATAG ATGGAGCCAT TGAGTCTTTC 
TCTCCCTTTC ACAATATCAA CTGCCCCAAA GGATTTCTCT ACTTCAACAA ACAGGGTGAG 
CTGAGGATCA GTGTGTTGCC AACATACCTC TCTTATGATG CTCCCTGGCC TGTGCGCAAG 
ATCCCTCTTA GGTGCACCGT CCACTATGTA TCATACCATG TGGAATCCAA GGTGTATGCA 
GTGTGCACCA GTGTTAAAGA GCCGTGCACG CGCATCCCCA GAATGACTGG AGAAGAGAAG 
GAATTTGAGA CCATTGAAAG AGACGAGCGA TATATTCACC CTCAACAGGA CAAGTTCTCA 
ATTCAGCTCA TCTCTCCAGT GAGCTGGGAG GCGATTCCAA ACACACGCGT TGACCTTGAG 
GAGTGGGAGC ATGTGACGTG CATGAAGACG GTGGCTCTGA AGAGTCAGGA GACAGTGTCA 
GGACTAAAGG GATACGTGGC TCTTGGGACG TGTCTCATGC AGGGAGAAGA GGTCACCTGT 
AGAGGCAGAA TCCTGATTCT GGATGTGATA GAGGTGGTCC CAGAGCCCGG ACAGCCCCTC 
ACCAAGAACA AGTTTAAAGT GCTGTATGAA AAGGAGCAGA AAGGTCCCGT CACTGCTCTT 
TGTCACTGCA GTGGATTCCT GGTGTCTGCT ATTGGACAGA AGATCTTCCT GTGGAGTCTG 
AAGGATAATG ATCTGACGGG AATGGCATTC ATTGACACAC AGCTCTACAT TCATCAGATG 
TACAGCATCA AGAACTTCAT CCTCGCAGCA GATGTGATGA AGAGCATCTC TCTGCTGCGC 
TACCAGCCGG AGAGCAAGAC GCTTTCACTC GTCAGCAGGG ATGCAAAGCC CCTTGAGGTT 
TACAGCATAG AGTTTATGGT AGACAATAAC CAGCTTGGTT TTTTGGTTTC AGACAGAGAC 
AAAAACCTTA TGGTCTACAT GTACCTGCCT GAAGCCAAAG AGAGTTTTGG AGGCATGCGT 
TTGTTGCGGA GAGCAGATTT TAACGTGGGA TCGCATGTGA ACGCCTTCTG GAGGATGCCA 
TGCCGCGGGA CACTGGACAC AGCCAACAAG AAGGCCCTCA CCTGGGACAA CAAACACATC 
ACCTGGTTTG CTACGCTGGA TGGTGGTGTC GGTCTGCTTT TACCAATGCA GGAGAAGACG 
TACCGCAGAC TGCTGATGTT GCAGAACGCT CTCACCACCA TGCTGCCACA TCATGCCGGG 
CTCAATCCAA AGGCTTTCAG GATGCTGCAC TGTGACCGGC GAACGCTCCA GAATGCTGTC 
AAGAACATCC TGGATGGAGA GCTGCTCAAT AAATACCTGT ATCTCAGTAC CATGGAGAGA 
AGTGAACTGG CCAAGAAGAT CGGCACGACC CCTGACATTA TCTTGGATGA TCTTCTGGAG 
ATTGAAAGAG TCACTGCTCA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CPSF1 mutations are associated with early-onset high myopia and involved in retinal ganglion cell axon projection.
Human molecular genetics28(12)1959-1970(2019 06)
Ouyang J,Sun W,Xiao X,Li S,Jia X,Zhou L,Wang P,Zhang Q

Single-cell transcriptional analysis of normal, aberrant, and malignant hematopoiesis in zebrafish.
The Journal of experimental medicine213(6)979-92(2016 05)
Moore FE,Garcia EG,Lobbardi R,Jain E,Tang Q,Moore JC,Cortes M,Molodtsov A,Kasheta M,Luo CC,Garcia AJ,Mylvaganam R,Yoder JA,Blackburn JS,Sadreyev RI,Ceol CJ,North TE,Langenau DM

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

cpsf1 is required for definitive HSC survival in zebrafish.
Blood117(15)3996-4007(2011 Apr)
Bolli N,Payne EM,Rhodes J,Gjini E,Johnston AB,Guo F,Lee JS,Stewart RA,Kanki JP,Chen AT,Zhou Y,Zon LI,Look AT

Identification of 315 genes essential for early zebrafish development.
Proceedings of the National Academy of Sciences of the United States of America101(35)12792-7(2004 Aug)
Amsterdam A,Nissen RM,Sun Z,Swindell EC,Farrington S,Hopkins N

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cpsf1 cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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