zgc:55262 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	zgc:55262
Entrez Gene ID	406768
Full Name	zgc:55262
Synonyms	Capn1,fj67g01,wu:fj67g01
General protein information	Preferred Names zgc:55262 Names calpain 1, large subunit calpain 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_213459.1	NP_998624.1	calpain 1, large subunit

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CAPN1	XP_001169100.1
Mus musculus (house mouse)	Capn1	NP_001103974.1
Bos taurus (cattle)	CAPN1	NP_776684.1
Danio rerio (zebrafish)	si:dkeyp-50d11.2	XP_689397.5
Xenopus tropicalis (tropical clawed frog)	capn1	NP_001013632.1
Gallus gallus (chicken)	CAPN1	NP_001038137.1
Danio rerio (zebrafish)	zgc:55262	NP_998624.1
Homo sapiens (human)	CAPN1	NP_001185797.1
Macaca mulatta (Rhesus monkey)	CAPN1	XP_001114172.1
Canis lupus familiaris (dog)	CAPN1	XP_540866.2
Rattus norvegicus (Norway rat)	Capn1	NP_062025.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Molecular description of eye defects in the zebrafish Pax6b mutant, sunrise, reveals a Pax6b-dependent genetic network in the developing anterior chamber.
Takamiya M, Weger BD, Schindler S, Beil T, Yang L, Armant O, Ferg M, Schlunck G, Reinhard T, Dickmeis T, Rastegar S, Strähle U
PloS one10(2)e0117645(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following zgc:55262 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the zgc:55262 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa36276	NM_213459.1 Latest version!	Danio rerio zgc:55262 (zgc:55262), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

zgc:55262 ( NM_213459.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_998624.1 Danio rerio zgc:55262 (zgc:55262), mRNA.

Price & Availability↑

CloneID	ODa36276
Clone ID Related Accession (Same CDS sequence)	NM_213459.1
Accession Version	NM_213459.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1542902400000
Organism	Danio rerio(zebrafish)
Product	calpain 1, large subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC048025.1. On Aug 18, 2004 this sequence version replaced NM_213551.1. ##Evidence-Data-START## Transcript exon combination :: BC048025.1, AF282675.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGAGCCCA TCTGCGCCAC TGGAATGGCT GCGAGGCTGC GGAGTCAGTG GGATCGAGAC 
GCGGGTTTGG GTCAAAACCA CAACGCTGTC AAGTTCTTGG GTCAAGATTA CGAGACGCTG 
AGAGCTCAGA GCCAGCAGAG CAGACGACTG TTTGAGGATC CAATGTTCAC CGCCAGCAGC 
TCTTCCCTGG GCTTCAATGA ACTCGGCCCG CGATCCTCCA AAACACAAGG AGTGCGCTGG 
ATGAGACCCA AGGAAATGTG TGCGCGTCCT CAGTTTATCG TGGATGGAGC CACTCGCACA 
GACATCTGTC AGGGAGCGCT GGGTGATTGT TGGCTGCTGG CTGCTATCGC GTCCCTGACT 
CTGAATGATA ATCTGCTGCA TCGCGTGGTG CCGCACGGAC AGGACTTTGA CAGCAGATAT 
GCAGGAATAT TCCACTTCCA GTTCTGGCAG TTTGGTGAAT GGGTGGACGT GGTGATCGAC 
GACCGGCTGC CGACGAAGGA TGGCAAACTG TTATTCGTGC ACTCGGCGGA GGGTGGAGAG 
TTCTGGAGTG CTCTGCTGGA GAAGGCCTAC GCCAAGCTGA ACGGCTGTTA CGAGGCTCTG 
TCTGGAGGCA GCACGTGTGA AGGCTTCGAG GATTTCACCG GTGGAGTGAC AGAGATGTAC 
GAGTTAAAGA AAGCTCCCGC TGACCTCTTC AGCATTATCG GCCGCGCCAT CGAGAGAGGC 
TCGCTGCTGG GCTGCTCTAT TGATATCACC AGTAAGTTTG ACATGGAGGC GGTGACCTTT 
AAGAAGCTGG TCAAAGGCCA CGCATATTCT GTGACCGGAG CAGAAGAGGT GGTCTACAGG 
GGGAACATGA CCAAACTGGT GCGCATCAGG AACCCGTGGG GGGAAGTGGA GTGGACTGGA 
GCCTGGAGCG ACAACTCTCG CGAATGGGAC AATGTGGACC GCTCCGTCAG GGGCCGACTA 
CAGAACCGCA GTGAGGACGG AGAGTTCTGG ATGTCGTTCA GTGATTTTCT GCGCGAGTTC 
ACCCGTTTGG AAATCTGTAA CCTGACGGCG GACGCTCTCC AGGCCAGTCA GGTGAAGAAG 
TGGAGCACCG CCAACTATAA CGGCGAGTGG AGGAGAGGAA GCACCGCCGG CGGCTGCAGG 
AACTATCCAG CAACATTCTG GAACAACCCG CAGTTCAAGG TGGCCCTGAA ACACCCGGAT 
TCTCCCGGCC AATCAGAGTG CAGCTTCCTG GTGGCGCTGA TGCAGAAGGA CCGCAGGAAG 
AAGAGGAGAG AAGGGCAGGA CATGGAGACT ATCGGATTCG CCATTTATGA GGTGCCAAGA 
GAGTTTCTGG GTCAGTCTGG TGTTCATCTG AAGCGGGATT TCTTCCTCAC ACACGCATCC 
AGCGCTCGAT CGGAGCTCTT CATTAACCTG CGGGAGGTGA GCTCCCGTTT CCGGCTGCCC 
GCGGGCGAGT ACATCATCGT CCCGTCCACC TTCGAGCCAA ACAAAGAGGC CGATTTCGTG 
CTCAGAGTTT TCTCAGAGAA GCCCGCCAAC TCAGAAGAAA TGGATGACAA AGTCATGGCT 
GAAATTCCTG AGGAGCAACG TCTTGATGAG AGTCAGATTG ACGCTGGTTT TAAGAGTCTG 
TTCAGACAGC TGGCTGGAGC GGACATGGAG ATCAGTGTGA CGGAGCTACA GACGATCCTG 
AACAGAATCA TCGCCAAACA TAAAGATTTA AAGACAGACG GCTTTGGTAA GGAGTCGTGT 
CGGAGTATGA TCAACCTCGT GGACACGGAT GGCAGTGGGA AACTCGGACT CGTAGAGTTT 
CATGTGTTGT GGGAGAAGAT TAAACGCTAC CTGCAAATCT TCCGCGATCA CGATGTGGAT 
AAATCAGGCA CTATGAGCTC GTATGAGATG AGGAAAGCTC TGGAAACTGC AGGATTTAAG 
CTGAACAATC ATTTATTCCA GCTGATCATC CTGCGCTACA CTGAGGAGGA TCTTTCCGTG 
GATTTTGACA ACTTTGTGTC CTGTTTAGTG CGACTGGAGA CCATGTTCAA GACGTTCAAG 
AGTCTGGACA CAGACGCTGA TGGAGTCATT TCACTCACAT TCTTCCAGTG GATCACCCTG 
ACCATGTTTG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998624.1
CDS	219..2333
Translation	MEPICATGMAARLRSQWDRDAGLGQNHNAVKFLGQDYETLRAQSQQSRRLFEDPMFTASSSSLGFNELGPRSSKTQGVRWMRPKEMCARPQFIVDGATRTDICQGALGDCWLLAAIASLTLNDNLLHRVVPHGQDFDSRYAGIFHFQFWQFGEWVDVVIDDRLPTKDGKLLFVHSAEGGEFWSALLEKAYAKLNGCYEALSGGSTCEGFEDFTGGVTEMYELKKAPADLFSIIGRAIERGSLLGCSIDITSKFDMEAVTFKKLVKGHAYSVTGAEEVVYRGNMTKLVRIRNPWGEVEWTGAWSDNSREWDNVDRSVRGRLQNRSEDGEFWMSFSDFLREFTRLEICNLTADALQASQVKKWSTANYNGEWRRGSTAGGCRNYPATFWNNPQFKVALKHPDSPGQSECSFLVALMQKDRRKKRREGQDMETIGFAIYEVPREFLGQSGVHLKRDFFLTHASSARSELFINLREVSSRFRLPAGEYIIVPSTFEPNKEADFVLRVFSEKPANSEEMDDKVMAEIPEEQRLDESQIDAGFKSLFRQLAGADMEISVTELQTILNRIIAKHKDLKTDGFGKESCRSMINLVDTDGSGKLGLVEFHVLWEKIKRYLQIFRDHDVDKSGTMSSYEMRKALETAGFKLNNHLFQLIILRYTEEDLSVDFDNFVSCLVRLETMFKTFKSLDTDADGVISLTFFQWITLTMFA

Target ORF information:

RefSeq Version	NM_213459.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio zgc:55262 (zgc:55262), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213459.1

ORF Insert Sequence:

ATGGAGCCCA TCTGCGCCAC TGGAATGGCT GCGAGGCTGC GGAGTCAGTG GGATCGAGAC 
GCGGGTTTGG GTCAAAACCA CAACGCTGTC AAGTTCTTGG GTCAAGATTA CGAGACGCTG 
AGAGCTCAGA GCCAGCAGAG CAGACGACTG TTTGAGGATC CAATGTTCAC CGCCAGCAGC 
TCTTCCCTGG GCTTCAATGA ACTCGGCCCG CGATCCTCCA AAACACAAGG AGTGCGCTGG 
ATGAGACCCA AGGAAATGTG TGCGCGTCCT CAGTTTATCG TGGATGGAGC CACTCGCACA 
GACATCTGTC AGGGAGCGCT GGGTGATTGT TGGCTGCTGG CTGCTATCGC GTCCCTGACT 
CTGAATGATA ATCTGCTGCA TCGCGTGGTG CCGCACGGAC AGGACTTTGA CAGCAGATAT 
GCAGGAATAT TCCACTTCCA GTTCTGGCAG TTTGGTGAAT GGGTGGACGT GGTGATCGAC 
GACCGGCTGC CGACGAAGGA TGGCAAACTG TTATTCGTGC ACTCGGCGGA GGGTGGAGAG 
TTCTGGAGTG CTCTGCTGGA GAAGGCCTAC GCCAAGCTGA ACGGCTGTTA CGAGGCTCTG 
TCTGGAGGCA GCACGTGTGA AGGCTTCGAG GATTTCACCG GTGGAGTGAC AGAGATGTAC 
GAGTTAAAGA AAGCTCCCGC TGACCTCTTC AGCATTATCG GCCGCGCCAT CGAGAGAGGC 
TCGCTGCTGG GCTGCTCTAT TGATATCACC AGTAAGTTTG ACATGGAGGC GGTGACCTTT 
AAGAAGCTGG TCAAAGGCCA CGCATATTCT GTGACCGGAG CAGAAGAGGT GGTCTACAGG 
GGGAACATGA CCAAACTGGT GCGCATCAGG AACCCGTGGG GGGAAGTGGA GTGGACTGGA 
GCCTGGAGCG ACAACTCTCG CGAATGGGAC AATGTGGACC GCTCCGTCAG GGGCCGACTA 
CAGAACCGCA GTGAGGACGG AGAGTTCTGG ATGTCGTTCA GTGATTTTCT GCGCGAGTTC 
ACCCGTTTGG AAATCTGTAA CCTGACGGCG GACGCTCTCC AGGCCAGTCA GGTGAAGAAG 
TGGAGCACCG CCAACTATAA CGGCGAGTGG AGGAGAGGAA GCACCGCCGG CGGCTGCAGG 
AACTATCCAG CAACATTCTG GAACAACCCG CAGTTCAAGG TGGCCCTGAA ACACCCGGAT 
TCTCCCGGCC AATCAGAGTG CAGCTTCCTG GTGGCGCTGA TGCAGAAGGA CCGCAGGAAG 
AAGAGGAGAG AAGGGCAGGA CATGGAGACT ATCGGATTCG CCATTTATGA GGTGCCAAGA 
GAGTTTCTGG GTCAGTCTGG TGTTCATCTG AAGCGGGATT TCTTCCTCAC ACACGCATCC 
AGCGCTCGAT CGGAGCTCTT CATTAACCTG CGGGAGGTGA GCTCCCGTTT CCGGCTGCCC 
GCGGGCGAGT ACATCATCGT CCCGTCCACC TTCGAGCCAA ACAAAGAGGC CGATTTCGTG 
CTCAGAGTTT TCTCAGAGAA GCCCGCCAAC TCAGAAGAAA TGGATGACAA AGTCATGGCT 
GAAATTCCTG AGGAGCAACG TCTTGATGAG AGTCAGATTG ACGCTGGTTT TAAGAGTCTG 
TTCAGACAGC TGGCTGGAGC GGACATGGAG ATCAGTGTGA CGGAGCTACA GACGATCCTG 
AACAGAATCA TCGCCAAACA TAAAGATTTA AAGACAGACG GCTTTGGTAA GGAGTCGTGT 
CGGAGTATGA TCAACCTCGT GGACACGGAT GGCAGTGGGA AACTCGGACT CGTAGAGTTT 
CATGTGTTGT GGGAGAAGAT TAAACGCTAC CTGCAAATCT TCCGCGATCA CGATGTGGAT 
AAATCAGGCA CTATGAGCTC GTATGAGATG AGGAAAGCTC TGGAAACTGC AGGATTTAAG 
CTGAACAATC ATTTATTCCA GCTGATCATC CTGCGCTACA CTGAGGAGGA TCTTTCCGTG 
GATTTTGACA ACTTTGTGTC CTGTTTAGTG CGACTGGAGA CCATGTTCAA GACGTTCAAG 
AGTCTGGACA CAGACGCTGA TGGAGTCATT TCACTCACAT TCTTCCAGTG GATCACCCTG 
ACCATGTTTG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Molecular description of eye defects in the zebrafish Pax6b mutant, sunrise, reveals a Pax6b-dependent genetic network in the developing anterior chamber.
PloS one10(2)e0117645(2015)
Takamiya M,Weger BD,Schindler S,Beil T,Yang L,Armant O,Ferg M,Schlunck G,Reinhard T,Dickmeis T,Rastegar S,Strähle U

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zgc:55262 cDNA ORF clone, Danio rerio(zebrafish)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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