sqstm1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	sqstm1
Entrez Gene ID	406452
Full Name	sequestosome 1
Synonyms	sb:cb621,zgc:85784
General protein information	Preferred Names sequestosome 1 Names sequestosome-1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001312913.1	NP_001299842.1	sequestosome-1

Pathways from BioSystems

Homologies

Homo sapiens (human)	SQSTM1	NP_003891.1
Macaca mulatta (Rhesus monkey)	SQSTM1	XP_001102347.1
Mus musculus (house mouse)	Sqstm1	NP_035148.1
Pan troglodytes (chimpanzee)	SQSTM1	XP_001153075.1
Bos taurus (cattle)	SQSTM1	NP_788814.1
Gallus gallus (chicken)	SQSTM1	XP_001233249.2
Rattus norvegicus (Norway rat)	Sqstm1	NP_787037.2
Xenopus tropicalis (tropical clawed frog)	sqstm1	NP_001007894.1
Danio rerio (zebrafish)	sqstm1	NP_998338.1

Gene Ontology
Bibliography

Related articles in PubMed

Zebrafish modeling of intestinal injury, bacterial exposures and medications defines epithelial
Chuang LS, Morrison J, Hsu NY, Labrias PR, Nayar S, Chen E, Villaverde N, Facey JA, Boschetti G, Giri M, Castillo-Martin M, Thin TH, Sharma Y, Chu J, Cho JH
Disease models & mechanisms12(8)(2019 08)

Hedgehog signaling regulates osteoblast differentiation in zebrafish larvae through modulation of autophagy.
Hu Z, Chen B, Zhao Q
Biology open8(5)(2019 May)

In vitro and in vivo studies of oxidative stress responses against acrylamide toxicity in zebrafish.
Komoike Y, Matsuoka M
Journal of hazardous materials365430-439(2019 Mar)

The selective autophagy receptors Optineurin and p62 are both required for zebrafish host resistance to mycobacterial infection.
Zhang R, Varela M, Vallentgoed W, Forn-Cuni G, van der Vaart M, Meijer AH
PLoS pathogens15(2)e1007329(2019 02)

The Lysosomal Transcription Factor TFEB Represses Myelination Downstream of the Rag-Ragulator Complex.
Meireles AM, Shen K, Zoupi L, Iyer H, Bouchard EL, Williams A, Talbot WS
Developmental cell47(3)319-330.e5(2018 11)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following sqstm1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sqstm1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa19439	NM_001312913.1 Latest version!	Danio rerio sequestosome 1 (sqstm1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sqstm1 ( NM_001312913.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001299842.1 Danio rerio sequestosome 1 (sqstm1), mRNA.

Price & Availability↑

CloneID	ODa19439
Clone ID Related Accession (Same CDS sequence)	NM_001312913.1
Accession Version	NM_001312913.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575648000000
Organism	Danio rerio(zebrafish)
Product	sequestosome-1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU075768.4, EH505876.1, DR724012.1 and EH510853.1. On Aug 22, 2015 this sequence version replaced XM_005157212.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GFIL01039635.1, KC513821.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGATGA CAGTGAAAGC TTACCTTATC GGTAAGGAGG ACTGCAACAA GGAGATCCGT 
CGCTTCGCCG TGGATCAAGA TGTCTCAACA AGTTTCGAGT ATTTGCAGCG AAAAGTGCTC 
GATGTTTTTG TCGGTCTCAG GACTGCGCCC TTCCAAATGT ACTATAAAGA TGAAGATGGT 
GATATGATTG CCTTTTCCTC TGACGATGAG CTAATGATGG GTTTGGCTCT TGTGAAGGAT 
GACACTTTCC GTCTCTTCAT CAAGCAACGA AAAGAGCACA AACGTGATTC TTCTCCTCAT 
GGAATTCCTG GGTTTCCGTT CACTACACCT CACTTTAGCC CTCCTGGTCC CTGTCATATG 
GGTCCATCTC CAATGGGGCC TCCAGGACCA CCTCATATGG GGGGTCCTCC TCACCACCCT 
CACCCAATGG TGCACCCAGG TGTGACCTGT GACGGGTGTG ATGGGCCGGT GGCTGGAACC 
CGTTACAAGT GCACCGTTTG CCCCGATTAT GACTTGTGCC CCACCTGCCA GAGCAAAGGC 
CTGCACAAGG AGCATGCCCT CCTGCCCATC TTTCATCCTA TGGCCAACGT GTTTGAGTGG 
CTCCCTCGTG GGAAATTTTG GCGTAAGATG AGACACTGCA TGTGGGCAAA TGCTCAAGCT 
CAGGCTCAAA ACCAGCCTCA GCCAGGTCCA TCTGGAGCGC AGCAAAACCA GGATGCTCCT 
GAAAACCCAA ATGAGAATGG TGCTACTGCC TCTTCTCAGG CTAACGTGGA GTACCTAAAG 
AACATCGGAG AAGAAGTGGC AGCCATGTTA AGCCCCCTTG GCATAGATGT GGACATCGAT 
GTTGAGCATG AGGGAAAGCG GACCAAAGTA ACCCCAACTC CTCCTGCCTC CAGTGGACCA 
TCCAGTGCTC GGAGTGACAG TGGTTCTGTG GGGCTTCTTT CTAGAGGCTC TGGTCCAGGA 
AGTCAGGCCA CTGAAGAGAG CGTCAGTGAG GGAACAAAGA AAGACCAATG TAGTGATGAG 
GAATGGACTC ATCTTAGTGC TAAAGAGGTG GATCCTTCGA CAGGAGAGCT GCAGTCTCTT 
AGACTAGAGC AGGACGGGGC AGATCTTCCA GCTCCACTAA ATACTGCTTC TGGCACAAGC 
ACCAGAGACG GTCCCACTGG TCTGCGCGAG GCAGCTCTCT ACCCACACCT TCCTCAAGAT 
GCAGACCCTA GGCTGGTGGA GTCTCTGTCT CAGATGCTCT CCATGGGTTT CACCGATGAG 
GGTGGATGGC TAACCAGACT GCTCCACACC AAGAATTACG ACATCGGTGG TGCTCTGGAC 
ACCATCCAGT ACTCCAAAAC ACCAGGACAA CAGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001299842.1
CDS	131..1489
Translation	MSMTVKAYLIGKEDCNKEIRRFAVDQDVSTSFEYLQRKVLDVFVGLRTAPFQMYYKDEDGDMIAFSSDDELMMGLALVKDDTFRLFIKQRKEHKRDSSPHGIPGFPFTTPHFSPPGPCHMGPSPMGPPGPPHMGGPPHHPHPMVHPGVTCDGCDGPVAGTRYKCTVCPDYDLCPTCQSKGLHKEHALLPIFHPMANVFEWLPRGKFWRKMRHCMWANAQAQAQNQPQPGPSGAQQNQDAPENPNENGATASSQANVEYLKNIGEEVAAMLSPLGIDVDIDVEHEGKRTKVTPTPPASSGPSSARSDSGSVGLLSRGSGPGSQATEESVSEGTKKDQCSDEEWTHLSAKEVDPSTGELQSLRLEQDGADLPAPLNTASGTSTRDGPTGLREAALYPHLPQDADPRLVESLSQMLSMGFTDEGGWLTRLLHTKNYDIGGALDTIQYSKTPGQQK

Target ORF information:

RefSeq Version	NM_001312913.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sequestosome 1 (sqstm1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001312913.1

ORF Insert Sequence:

ATGTCGATGA CAGTGAAAGC TTACCTTATC GGTAAGGAGG ACTGCAACAA GGAGATCCGT 
CGCTTCGCCG TGGATCAAGA TGTCTCAACA AGTTTCGAGT ATTTGCAGCG AAAAGTGCTC 
GATGTTTTTG TCGGTCTCAG GACTGCGCCC TTCCAAATGT ACTATAAAGA TGAAGATGGT 
GATATGATTG CCTTTTCCTC TGACGATGAG CTAATGATGG GTTTGGCTCT TGTGAAGGAT 
GACACTTTCC GTCTCTTCAT CAAGCAACGA AAAGAGCACA AACGTGATTC TTCTCCTCAT 
GGAATTCCTG GGTTTCCGTT CACTACACCT CACTTTAGCC CTCCTGGTCC CTGTCATATG 
GGTCCATCTC CAATGGGGCC TCCAGGACCA CCTCATATGG GGGGTCCTCC TCACCACCCT 
CACCCAATGG TGCACCCAGG TGTGACCTGT GACGGGTGTG ATGGGCCGGT GGCTGGAACC 
CGTTACAAGT GCACCGTTTG CCCCGATTAT GACTTGTGCC CCACCTGCCA GAGCAAAGGC 
CTGCACAAGG AGCATGCCCT CCTGCCCATC TTTCATCCTA TGGCCAACGT GTTTGAGTGG 
CTCCCTCGTG GGAAATTTTG GCGTAAGATG AGACACTGCA TGTGGGCAAA TGCTCAAGCT 
CAGGCTCAAA ACCAGCCTCA GCCAGGTCCA TCTGGAGCGC AGCAAAACCA GGATGCTCCT 
GAAAACCCAA ATGAGAATGG TGCTACTGCC TCTTCTCAGG CTAACGTGGA GTACCTAAAG 
AACATCGGAG AAGAAGTGGC AGCCATGTTA AGCCCCCTTG GCATAGATGT GGACATCGAT 
GTTGAGCATG AGGGAAAGCG GACCAAAGTA ACCCCAACTC CTCCTGCCTC CAGTGGACCA 
TCCAGTGCTC GGAGTGACAG TGGTTCTGTG GGGCTTCTTT CTAGAGGCTC TGGTCCAGGA 
AGTCAGGCCA CTGAAGAGAG CGTCAGTGAG GGAACAAAGA AAGACCAATG TAGTGATGAG 
GAATGGACTC ATCTTAGTGC TAAAGAGGTG GATCCTTCGA CAGGAGAGCT GCAGTCTCTT 
AGACTAGAGC AGGACGGGGC AGATCTTCCA GCTCCACTAA ATACTGCTTC TGGCACAAGC 
ACCAGAGACG GTCCCACTGG TCTGCGCGAG GCAGCTCTCT ACCCACACCT TCCTCAAGAT 
GCAGACCCTA GGCTGGTGGA GTCTCTGTCT CAGATGCTCT CCATGGGTTT CACCGATGAG 
GGTGGATGGC TAACCAGACT GCTCCACACC AAGAATTACG ACATCGGTGG TGCTCTGGAC 
ACCATCCAGT ACTCCAAAAC ACCAGGACAA CAGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Zebrafish modeling of intestinal injury, bacterial exposures and medications defines epithelial
Disease models & mechanisms12(8)(2019 08)
Chuang LS,Morrison J,Hsu NY,Labrias PR,Nayar S,Chen E,Villaverde N,Facey JA,Boschetti G,Giri M,Castillo-Martin M,Thin TH,Sharma Y,Chu J,Cho JH

Hedgehog signaling regulates osteoblast differentiation in zebrafish larvae through modulation of autophagy.
Biology open8(5)(2019 May)
Hu Z,Chen B,Zhao Q

In vitro and in vivo studies of oxidative stress responses against acrylamide toxicity in zebrafish.
Journal of hazardous materials365430-439(2019 Mar)
Komoike Y,Matsuoka M

The selective autophagy receptors Optineurin and p62 are both required for zebrafish host resistance to mycobacterial infection.
PLoS pathogens15(2)e1007329(2019 02)
Zhang R,Varela M,Vallentgoed W,Forn-Cuni G,van der Vaart M,Meijer AH

The Lysosomal Transcription Factor TFEB Represses Myelination Downstream of the Rag-Ragulator Complex.
Developmental cell47(3)319-330.e5(2018 11)
Meireles AM,Shen K,Zoupi L,Iyer H,Bouchard EL,Williams A,Talbot WS