asxl1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	asxl1
Entrez Gene ID	403066
Full Name	additional sex combs like transcriptional regulator 1
Synonyms	si:dkey-202m22.4
General protein information	Preferred Names additional sex combs like transcriptional regulator 1 Names putative Polycomb group protein ASXL1 additional sex combs like 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021473723.1	XP_021329398.1	putative Polycomb group protein ASXL1 isoform X2
XM_021473722.1	XP_021329397.1	putative Polycomb group protein ASXL1 isoform X1
XM_001341206.5	XP_001341242.2	putative Polycomb group protein ASXL1 isoform X3
XM_021473724.1	XP_021329399.1	putative Polycomb group protein ASXL1 isoform X3
XM_021470008.1	XP_021325683.1	putative Polycomb group protein ASXL1 isoform X1
XM_005162338.1	XP_005162395.1	putative Polycomb group protein ASXL1 isoform X2

Pathways from BioSystems
Gene Ontology

The following asxl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the asxl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa146304	XM_021470008.1 Latest version!	Danio rerio additional sex combs like transcriptional regulator 1 (asxl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa146304	XM_021473722.1 Latest version!	Danio rerio additional sex combs like transcriptional regulator 1 (asxl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa27915	XM_021473723.1 Latest version!	Danio rerio additional sex combs like transcriptional regulator 1 (asxl1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa27915	XM_005162338.1 Latest version!	Danio rerio additional sex combs like transcriptional regulator 1 (asxl1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa27916	XM_001341206.5 Latest version!	Danio rerio additional sex combs like transcriptional regulator 1 (asxl1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa27916	XM_021473724.1 Latest version!	Danio rerio additional sex combs like transcriptional regulator 1 (asxl1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

asxl1 ( XM_021473722.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329397.1 Danio rerio additional sex combs like transcriptional regulator 1 (asxl1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa146304
Clone ID Related Accession (Same CDS sequence)	XM_021473722.1 , XM_021470008.1
Accession Version	XM_021473722.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	putative Polycomb group protein ASXL1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395158.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGACA AACAGAAGAG AAAGAAGGAG CGCACGTGGG CTGAGGCAGC TCGTATGGTT 
CTGGAGAACT TTTCTGATGC CCCAATGACG CCCAAACAGA TTCTACATGT CATCCAAACA 
AAGGGGTTGA AAGAAATGAG AAGCGGCACA GCACCACTGG CCTGCCTTGT CACCATGCTT 
CACTCTCAGG TCAGAGGTGA CCGAGTGAAA AACAGTATTT TCTTTAAGCT GCCTGGAAGG 
ATGAGTCTGT TTACCCTAAA GAAAAATGCA CTTCAGTGGA CAAAGAGCCC ATCAGCGAAT 
GAGTCTGGAG ATGGGACACC AACAGCATCC ACCACATCAA CAGTTGAAGG AGCAGAGCAG 
GAAAGCTGCG ACTCCACAGA GACGGCTGCT GCTAGTGGTG AAAATGATGC CTCTGTGGAT 
GAGACTTCCT CCAGTGCCTC TTGCTCTACA GAACCACAGA CAAGACTCAG TCGCTCTTCA 
CAGTCAGGGA GACAGAGGAA GAAGGCTGTG ATGATGCCCC GTGTAGTTCT CACTCCTCTC 
AAGGTCAACG GTGAACACGT CCCATCAGGA GCAGCGGGGA GGCGCAGGGA AGGCTCTAGG 
GGGGGTCCAG GCCCCACTCT CCGTACCCGC TCCGAGCTGG GCTGGAAACG CACCCAGCAC 
TTCAAGAGCA TTCGTGGTCT TCGTTCAGGT ACAGAGGATG AAAACCAAGG ACCCCCATGT 
AGTCCCATGA AAAGGAGTCG AGGAGGAGTG GAGGTGGATT TTGAGACTCC AGGATCCATC 
CTGGTCAACA CCAACATTCG TGCTCTCATC AACACACGGA CCTTTGCTGC TTTTCCTTCT 
CACTCCCAGC AGCAACTACT CCAGCTTTTA CCAGAGGTGG ACAGACAGGT CGGCCCCGAT 
GGCCTTGCTC GTCTAAGCAG TTCGGCCCTC AATAATGAAT TCTTCACTCA TGCCTCCCAG 
AGCTGGAAAG AACGTCTAGC TGAGGGTGAG TTTACACATG AGATGCAGGT TCGCTTCAGG 
CAGGAGATGG AAAAGGAGAA GAAAGTAGAA GCATGGAAGG AGAAATTCTT CGAGGAGTAC 
CATGGCCAAA GGTCTGGCTT AACAAGAGAG GAGGCATTGA AGCTCACAAT GAGCGAAGCT 
GGAGATGTTG CTAGTCCAGT GCTAGACTCC GATTCTGCTA CAGTGGCTAC ACCCAAGAGG 
CGAAGTGTTG GAAGAAGACG GAGAGAAGGC CGTATCAGAC GACGTTCACG GGCTGACCTC 
AGGCGCAGGG CAAGGCGCAC ACTTTGCAAA ACTACAACTG CAACTGTGGC TACAGAAGAA 
ACAACAGAGA GCACTGCAAC TCCAGAAGAG GTACCATCAG TTGCATCACC AGTCCCAGAA 
ACCACAGCAG TTCAAGGAGA GGTGGTCCTC CAAACAGACC CTGAACTTGA AGCACCAGAA 
GAAAACCTCT CTACAGAGGC AGTACCTTCT CCAGTTCCTG TACCCACACC TCCTCCAGCA 
TCGACAAGCT CAACCTGCGA TGAGCCAGAA ACCTCTACAC GCCTGTTGCC AGAAGTACCC 
GAACCAGCTG TTGCATCCAC CTCATCTCCT TCCTCATCCT CCTCTTCAAC ATCGTCATCA 
TCTTCACCAT CTTCCTCTCC ATCCTCTGCC TCAGACAGAC AGACTTTTGC TGCAAGCTCA 
GACTCCTCTT CTTCCTCTTC CTCCTCCACT GCTGCTGTTG CCACTGATCC GCTGGATGAT 
GGGGCTTCAG TCATTACCAC TGGCACAGCA GGCACTGGAG CAAGCAGCAG AGAAAGCAGC 
CCTGCAGCCA GTCCCGCTAC ATCAAGCCCA GCAATTCAGC TCAAGGAACA GAAGAGAAGA 
CCAGACGAGT CCCAGGCCTT CACCAGCTTT CCCGAGAAAA GGGCGCGGCT GGATGAGCGT 
CAGTCCTTTC GTAACACAGT TGACTGTGTG CACTCAGAAA AGCCACAGCC TACAACAGAG 
GAGCCCAAGG TCCCGCCAAT CCGGATACAG CTCTCCCGGA TCAAACCGCC CTGGGTCAAG 
GGGCCGCCAA CGTACCAAAT CTGTCCCCGC ATCGTCCCCC CCAACGAAGG GTCGCGGCGC 
TCTGGGACGG GGGCGCGCAC CTTGGCGGAC ATCAAAGCCC GTGCACAGCA AGCCCGTGCC 
CAGCGGGAAG CCGCTGCTGC TGTTGCAGCC TCTGGGGATG GGGGAGGGCC TGGGGGGAGC 
GGCCCGGGGG GTGGTACTGG GATACCGGAT CGCTCCAGCG GGAGGCGTAC AAGAGAGCAC 
CCAGGTCCCG TTGAACCTGG AGGAGGAGGA GGGAGACGAG GAGGAAGAGT TGATTTGGAG 
GAGCAGGAAT CGCCTGCGAG CTCTCATTCG TCGGGAGCAC AACTACAGCT GTCTAGTGTA 
GATTCGGCAG ACAGGCCTCA AACTTCCACC CCACTCACTG AACCATCTCC CTCTTCTGTG 
TCCTCAAACC CATCTCTACC ATCATCAGAA TCTCCAAAAA TACTTGCGCT ATCACCACCT 
GCAACCGACA GCCCTGCTTC CCAGGATCAG GTGGAGGAGA TTTGTGGAGT GGAAGAGGTG 
ACTGCCTGCC CAAGTGATAA AACCTCAGAG CAGACCTTAG ACACCCCAGT GCCTACCCCA 
AGTGAATCAG AGTCTTTTTG CAGTCACCAA GATGGGAGAG GCGAGGAAGC AGAGTCGAGT 
GATAGTAGAA CAAATACGCC TGTTTGCACC TCATCATCAA ATGATGCTAT TTCTTTAGTG 
CCCACCTCTA TACCTGACTC GCTACCAAGA TTTGGTGCCC AGGGTGTGGA TGTCATCAGG 
ACATTGGCAG CTTCCTCTCA GCCTTGGGAA GGTGAAAAGA AAGCGGTTGA GCTTCACCCA 
GGGACAACTG GAGTTATCCA GCATGGCTCA GACTTGAAAT TACCCAAAGA GACTCTTGTT 
ACAGCCAGAA ATGGATGGGT AGAAAATAGA GAAGAGCATT CCATGAGAGA GGGTATGCTA 
GAAGAACATA GGAATGGAGG GAGTGATGCT AATGGTAAAT ATGAATCAGG CTCTTTACCT 
TGCCTACCAC GATGTGCTGG TGAGGAAGAC ACTGGAGCAC ACAGTGATTC AACGGAGACA 
GCATCTGACT TTGAAAATGA ATCACAAGAG GATGAAATGG TGGATTGGCA TGGGACCCAG 
ATGGATGGTA GTGGCATGCA AACTCAAAAT ACAAAATCAC TGAATCGACC TGTTATTCAG 
ACCTCAAGTC GACTCACTTC ATCGATTGTT AATCCTCCTC AACACCAGCA ACCGGTTATA 
CAGGCCCATA TATCTAATCA AAACCATACC CAAACTGTTA TTCAGGCTTG CTTTCCTAAT 
GGTGTGCCAA ACCAACCAGT CATTCATCCT CACAAGCAGC ATGCAATTAA TACCCATGCT 
ATAAACCACT CTCAGGACCA GATTGCCTCT GCACCTTCAC AAGCCCAAGT GCAAGCTTAT 
CTGACCCAGA CTGAGATGGA CCAAAGCAGA AATCTACGAC TGAATGATAA TGGCAATGGT 
GTGAAGCTGT ATTTGTCAGA GGATGACACC AAACCTCTTG GCAGGGAAGA TTTAGTTTTA 
AAGAATTCTG CTGGTCCTCC AGTTGGCAGA AGAGTCCAAG TTTCATCTCG ACCAGTTTCC 
ACTGTAGAGG CCAACAATCC TCTTGTCACA CAGCTCCTCC AAGGCAGTCT ACCTTTAGAG 
AAGGTCTTAC CCCAACCCCA CTCAGCCAGC AAGTTAGAAA TTAATCGACT TCCAGGTGCC 
AATTCAAGCA ACTCGCTCAA CCCAGCCAAT CATCAAACAC AGAGAAATCT GGCACCACGT 
TTTAGAGGCC CACCTGAGTC GGGAGGGCCT ACGGACACTG TTAGTTCTGA CTTCCAACAC 
AAAACCCCAC CTGCTCGAGT ATCTCCTGGA CCTGGTCGGA ACTTTGGTTC TTCCCCGCCA 
GGTTCTGCTC CATCAAGAAT GGCATGCCTG TTTGAGGAAG CCAGCCCCAG GTCTGCAAAC 
TCTCAGTTCA GTTCTCAGCA ACCAGGAGGC GTTGTGCCTC CAGGAGCAGT ACCTGTCATA 
ACATCCCTTC CCTTAAACTC CACCACTAGA ACCTCAATGG ATATGAACTC TCAGAATGCT 
CCAGTTTATG AATCAGCTGT TATTAAAGAG CACCCTGGAC CTCTTTCTCA ACGGAGTGAA 
ACTCCTGAGA GGCCAAGTAG CTTTCAGCAG CATCCTAATA ATGTCTCCCA GTCCGTGTGC 
AGAAACACAC CAGATGCTCC TTCACCTCCA CATGGAGACC TTTGTCCATC AGAGGTTGTA 
CCAACTGTTA AGATTAACTG GCGCCCAAAC AAACCTCAGC AACCGATCTC CCCTGTAGCT 
ACAGTAAAAA ACGAAGTCTC CTTGCGCCCT TCGTGCCAAC AAGCTCTGAC CAAAAACTCA 
CCTGCTCCTA TCAGTGGCAA TACGTCTGTT GTAATAACCA AAAAGGAGCC TCTGGATGGT 
TTTCATGGAG GTGGTGGAGC TATGGAAGGA CTGCTAAATA TGGAAATGTC TCTTGTCAGA 
ATGGCCAAGA AGGAACAAGT CAAAAATCCA TATACCCGCC AAGCAGACAC CTCCGCTTCT 
CCTGTTTCTT CCTCTCCTTC CTCATCAGCC TCCAGTCTCC CTTTCCAGCT ATATGGTAAG 
CTACCAAAAC TTCAGCAGAG TGGGGGCAAC GGAGGGTCCT CATCCAGCTT CAGTTACACA 
GCTAATGTGT CTGTGGTGGA TGGTAGTGGC TTCACTCGCA CCTTAGCCGA TAGTGTGCTC 
CAACTAAGGC CACGTGTGAG CGTCGGCAGC GGTGGAGGTC AGAGTGCCAC GCTTGGCATC 
CAGGCATTTA CAGACAGTGC TGCCGAAGAA GTGGCTCTCA AGTGTTCCTG CCGACTCAAG 
GCTATGATCA TGTGCCAGGG CTGTGGCGCT TTCTGCCATG ATGATTGCAT CGGCCCTTCG 
AAACTGTGTG TGTCATGTCT GGTTGTCAGA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329397.1
CDS	483..5495
Translation	MKDKQKRKKERTWAEAARMVLENFSDAPMTPKQILHVIQTKGLKEMRSGTAPLACLVTMLHSQVRGDRVKNSIFFKLPGRMSLFTLKKNALQWTKSPSANESGDGTPTASTTSTVEGAEQESCDSTETAAASGENDASVDETSSSASCSTEPQTRLSRSSQSGRQRKKAVMMPRVVLTPLKVNGEHVPSGAAGRRREGSRGGPGPTLRTRSELGWKRTQHFKSIRGLRSGTEDENQGPPCSPMKRSRGGVEVDFETPGSILVNTNIRALINTRTFAAFPSHSQQQLLQLLPEVDRQVGPDGLARLSSSALNNEFFTHASQSWKERLAEGEFTHEMQVRFRQEMEKEKKVEAWKEKFFEEYHGQRSGLTREEALKLTMSEAGDVASPVLDSDSATVATPKRRSVGRRRREGRIRRRSRADLRRRARRTLCKTTTATVATEETTESTATPEEVPSVASPVPETTAVQGEVVLQTDPELEAPEENLSTEAVPSPVPVPTPPPASTSSTCDEPETSTRLLPEVPEPAVASTSSPSSSSSSTSSSSSPSSSPSSASDRQTFAASSDSSSSSSSSTAAVATDPLDDGASVITTGTAGTGASSRESSPAASPATSSPAIQLKEQKRRPDESQAFTSFPEKRARLDERQSFRNTVDCVHSEKPQPTTEEPKVPPIRIQLSRIKPPWVKGPPTYQICPRIVPPNEGSRRSGTGARTLADIKARAQQARAQREAAAAVAASGDGGGPGGSGPGGGTGIPDRSSGRRTREHPGPVEPGGGGGRRGGRVDLEEQESPASSHSSGAQLQLSSVDSADRPQTSTPLTEPSPSSVSSNPSLPSSESPKILALSPPATDSPASQDQVEEICGVEEVTACPSDKTSEQTLDTPVPTPSESESFCSHQDGRGEEAESSDSRTNTPVCTSSSNDAISLVPTSIPDSLPRFGAQGVDVIRTLAASSQPWEGEKKAVELHPGTTGVIQHGSDLKLPKETLVTARNGWVENREEHSMREGMLEEHRNGGSDANGKYESGSLPCLPRCAGEEDTGAHSDSTETASDFENESQEDEMVDWHGTQMDGSGMQTQNTKSLNRPVIQTSSRLTSSIVNPPQHQQPVIQAHISNQNHTQTVIQACFPNGVPNQPVIHPHKQHAINTHAINHSQDQIASAPSQAQVQAYLTQTEMDQSRNLRLNDNGNGVKLYLSEDDTKPLGREDLVLKNSAGPPVGRRVQVSSRPVSTVEANNPLVTQLLQGSLPLEKVLPQPHSASKLEINRLPGANSSNSLNPANHQTQRNLAPRFRGPPESGGPTDTVSSDFQHKTPPARVSPGPGRNFGSSPPGSAPSRMACLFEEASPRSANSQFSSQQPGGVVPPGAVPVITSLPLNSTTRTSMDMNSQNAPVYESAVIKEHPGPLSQRSETPERPSSFQQHPNNVSQSVCRNTPDAPSPPHGDLCPSEVVPTVKINWRPNKPQQPISPVATVKNEVSLRPSCQQALTKNSPAPISGNTSVVITKKEPLDGFHGGGGAMEGLLNMEMSLVRMAKKEQVKNPYTRQADTSASPVSSSPSSSASSLPFQLYGKLPKLQQSGGNGGSSSSFSYTANVSVVDGSGFTRTLADSVLQLRPRVSVGSGGGQSATLGIQAFTDSAAEEVALKCSCRLKAMIMCQGCGAFCHDDCIGPSKLCVSCLVVR

Target ORF information:

RefSeq Version	XM_021473722.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio additional sex combs like transcriptional regulator 1 (asxl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473722.1

ORF Insert Sequence:

ATGAAGGACA AACAGAAGAG AAAGAAGGAG CGCACGTGGG CTGAGGCAGC TCGTATGGTT 
CTGGAGAACT TTTCTGATGC CCCAATGACG CCCAAACAGA TTCTACATGT CATCCAAACA 
AAGGGGTTGA AAGAAATGAG AAGCGGCACA GCACCACTGG CCTGCCTTGT CACCATGCTT 
CACTCTCAGG TCAGAGGTGA CCGAGTGAAA AACAGTATTT TCTTTAAGCT GCCTGGAAGG 
ATGAGTCTGT TTACCCTAAA GAAAAATGCA CTTCAGTGGA CAAAGAGCCC ATCAGCGAAT 
GAGTCTGGAG ATGGGACACC AACAGCATCC ACCACATCAA CAGTTGAAGG AGCAGAGCAG 
GAAAGCTGCG ACTCCACAGA GACGGCTGCT GCTAGTGGTG AAAATGATGC CTCTGTGGAT 
GAGACTTCCT CCAGTGCCTC TTGCTCTACA GAACCACAGA CAAGACTCAG TCGCTCTTCA 
CAGTCAGGGA GACAGAGGAA GAAGGCTGTG ATGATGCCCC GTGTAGTTCT CACTCCTCTC 
AAGGTCAACG GTGAACACGT CCCATCAGGA GCAGCGGGGA GGCGCAGGGA AGGCTCTAGG 
GGGGGTCCAG GCCCCACTCT CCGTACCCGC TCCGAGCTGG GCTGGAAACG CACCCAGCAC 
TTCAAGAGCA TTCGTGGTCT TCGTTCAGGT ACAGAGGATG AAAACCAAGG ACCCCCATGT 
AGTCCCATGA AAAGGAGTCG AGGAGGAGTG GAGGTGGATT TTGAGACTCC AGGATCCATC 
CTGGTCAACA CCAACATTCG TGCTCTCATC AACACACGGA CCTTTGCTGC TTTTCCTTCT 
CACTCCCAGC AGCAACTACT CCAGCTTTTA CCAGAGGTGG ACAGACAGGT CGGCCCCGAT 
GGCCTTGCTC GTCTAAGCAG TTCGGCCCTC AATAATGAAT TCTTCACTCA TGCCTCCCAG 
AGCTGGAAAG AACGTCTAGC TGAGGGTGAG TTTACACATG AGATGCAGGT TCGCTTCAGG 
CAGGAGATGG AAAAGGAGAA GAAAGTAGAA GCATGGAAGG AGAAATTCTT CGAGGAGTAC 
CATGGCCAAA GGTCTGGCTT AACAAGAGAG GAGGCATTGA AGCTCACAAT GAGCGAAGCT 
GGAGATGTTG CTAGTCCAGT GCTAGACTCC GATTCTGCTA CAGTGGCTAC ACCCAAGAGG 
CGAAGTGTTG GAAGAAGACG GAGAGAAGGC CGTATCAGAC GACGTTCACG GGCTGACCTC 
AGGCGCAGGG CAAGGCGCAC ACTTTGCAAA ACTACAACTG CAACTGTGGC TACAGAAGAA 
ACAACAGAGA GCACTGCAAC TCCAGAAGAG GTACCATCAG TTGCATCACC AGTCCCAGAA 
ACCACAGCAG TTCAAGGAGA GGTGGTCCTC CAAACAGACC CTGAACTTGA AGCACCAGAA 
GAAAACCTCT CTACAGAGGC AGTACCTTCT CCAGTTCCTG TACCCACACC TCCTCCAGCA 
TCGACAAGCT CAACCTGCGA TGAGCCAGAA ACCTCTACAC GCCTGTTGCC AGAAGTACCC 
GAACCAGCTG TTGCATCCAC CTCATCTCCT TCCTCATCCT CCTCTTCAAC ATCGTCATCA 
TCTTCACCAT CTTCCTCTCC ATCCTCTGCC TCAGACAGAC AGACTTTTGC TGCAAGCTCA 
GACTCCTCTT CTTCCTCTTC CTCCTCCACT GCTGCTGTTG CCACTGATCC GCTGGATGAT 
GGGGCTTCAG TCATTACCAC TGGCACAGCA GGCACTGGAG CAAGCAGCAG AGAAAGCAGC 
CCTGCAGCCA GTCCCGCTAC ATCAAGCCCA GCAATTCAGC TCAAGGAACA GAAGAGAAGA 
CCAGACGAGT CCCAGGCCTT CACCAGCTTT CCCGAGAAAA GGGCGCGGCT GGATGAGCGT 
CAGTCCTTTC GTAACACAGT TGACTGTGTG CACTCAGAAA AGCCACAGCC TACAACAGAG 
GAGCCCAAGG TCCCGCCAAT CCGGATACAG CTCTCCCGGA TCAAACCGCC CTGGGTCAAG 
GGGCCGCCAA CGTACCAAAT CTGTCCCCGC ATCGTCCCCC CCAACGAAGG GTCGCGGCGC 
TCTGGGACGG GGGCGCGCAC CTTGGCGGAC ATCAAAGCCC GTGCACAGCA AGCCCGTGCC 
CAGCGGGAAG CCGCTGCTGC TGTTGCAGCC TCTGGGGATG GGGGAGGGCC TGGGGGGAGC 
GGCCCGGGGG GTGGTACTGG GATACCGGAT CGCTCCAGCG GGAGGCGTAC AAGAGAGCAC 
CCAGGTCCCG TTGAACCTGG AGGAGGAGGA GGGAGACGAG GAGGAAGAGT TGATTTGGAG 
GAGCAGGAAT CGCCTGCGAG CTCTCATTCG TCGGGAGCAC AACTACAGCT GTCTAGTGTA 
GATTCGGCAG ACAGGCCTCA AACTTCCACC CCACTCACTG AACCATCTCC CTCTTCTGTG 
TCCTCAAACC CATCTCTACC ATCATCAGAA TCTCCAAAAA TACTTGCGCT ATCACCACCT 
GCAACCGACA GCCCTGCTTC CCAGGATCAG GTGGAGGAGA TTTGTGGAGT GGAAGAGGTG 
ACTGCCTGCC CAAGTGATAA AACCTCAGAG CAGACCTTAG ACACCCCAGT GCCTACCCCA 
AGTGAATCAG AGTCTTTTTG CAGTCACCAA GATGGGAGAG GCGAGGAAGC AGAGTCGAGT 
GATAGTAGAA CAAATACGCC TGTTTGCACC TCATCATCAA ATGATGCTAT TTCTTTAGTG 
CCCACCTCTA TACCTGACTC GCTACCAAGA TTTGGTGCCC AGGGTGTGGA TGTCATCAGG 
ACATTGGCAG CTTCCTCTCA GCCTTGGGAA GGTGAAAAGA AAGCGGTTGA GCTTCACCCA 
GGGACAACTG GAGTTATCCA GCATGGCTCA GACTTGAAAT TACCCAAAGA GACTCTTGTT 
ACAGCCAGAA ATGGATGGGT AGAAAATAGA GAAGAGCATT CCATGAGAGA GGGTATGCTA 
GAAGAACATA GGAATGGAGG GAGTGATGCT AATGGTAAAT ATGAATCAGG CTCTTTACCT 
TGCCTACCAC GATGTGCTGG TGAGGAAGAC ACTGGAGCAC ACAGTGATTC AACGGAGACA 
GCATCTGACT TTGAAAATGA ATCACAAGAG GATGAAATGG TGGATTGGCA TGGGACCCAG 
ATGGATGGTA GTGGCATGCA AACTCAAAAT ACAAAATCAC TGAATCGACC TGTTATTCAG 
ACCTCAAGTC GACTCACTTC ATCGATTGTT AATCCTCCTC AACACCAGCA ACCGGTTATA 
CAGGCCCATA TATCTAATCA AAACCATACC CAAACTGTTA TTCAGGCTTG CTTTCCTAAT 
GGTGTGCCAA ACCAACCAGT CATTCATCCT CACAAGCAGC ATGCAATTAA TACCCATGCT 
ATAAACCACT CTCAGGACCA GATTGCCTCT GCACCTTCAC AAGCCCAAGT GCAAGCTTAT 
CTGACCCAGA CTGAGATGGA CCAAAGCAGA AATCTACGAC TGAATGATAA TGGCAATGGT 
GTGAAGCTGT ATTTGTCAGA GGATGACACC AAACCTCTTG GCAGGGAAGA TTTAGTTTTA 
AAGAATTCTG CTGGTCCTCC AGTTGGCAGA AGAGTCCAAG TTTCATCTCG ACCAGTTTCC 
ACTGTAGAGG CCAACAATCC TCTTGTCACA CAGCTCCTCC AAGGCAGTCT ACCTTTAGAG 
AAGGTCTTAC CCCAACCCCA CTCAGCCAGC AAGTTAGAAA TTAATCGACT TCCAGGTGCC 
AATTCAAGCA ACTCGCTCAA CCCAGCCAAT CATCAAACAC AGAGAAATCT GGCACCACGT 
TTTAGAGGCC CACCTGAGTC GGGAGGGCCT ACGGACACTG TTAGTTCTGA CTTCCAACAC 
AAAACCCCAC CTGCTCGAGT ATCTCCTGGA CCTGGTCGGA ACTTTGGTTC TTCCCCGCCA 
GGTTCTGCTC CATCAAGAAT GGCATGCCTG TTTGAGGAAG CCAGCCCCAG GTCTGCAAAC 
TCTCAGTTCA GTTCTCAGCA ACCAGGAGGC GTTGTGCCTC CAGGAGCAGT ACCTGTCATA 
ACATCCCTTC CCTTAAACTC CACCACTAGA ACCTCAATGG ATATGAACTC TCAGAATGCT 
CCAGTTTATG AATCAGCTGT TATTAAAGAG CACCCTGGAC CTCTTTCTCA ACGGAGTGAA 
ACTCCTGAGA GGCCAAGTAG CTTTCAGCAG CATCCTAATA ATGTCTCCCA GTCCGTGTGC 
AGAAACACAC CAGATGCTCC TTCACCTCCA CATGGAGACC TTTGTCCATC AGAGGTTGTA 
CCAACTGTTA AGATTAACTG GCGCCCAAAC AAACCTCAGC AACCGATCTC CCCTGTAGCT 
ACAGTAAAAA ACGAAGTCTC CTTGCGCCCT TCGTGCCAAC AAGCTCTGAC CAAAAACTCA 
CCTGCTCCTA TCAGTGGCAA TACGTCTGTT GTAATAACCA AAAAGGAGCC TCTGGATGGT 
TTTCATGGAG GTGGTGGAGC TATGGAAGGA CTGCTAAATA TGGAAATGTC TCTTGTCAGA 
ATGGCCAAGA AGGAACAAGT CAAAAATCCA TATACCCGCC AAGCAGACAC CTCCGCTTCT 
CCTGTTTCTT CCTCTCCTTC CTCATCAGCC TCCAGTCTCC CTTTCCAGCT ATATGGTAAG 
CTACCAAAAC TTCAGCAGAG TGGGGGCAAC GGAGGGTCCT CATCCAGCTT CAGTTACACA 
GCTAATGTGT CTGTGGTGGA TGGTAGTGGC TTCACTCGCA CCTTAGCCGA TAGTGTGCTC 
CAACTAAGGC CACGTGTGAG CGTCGGCAGC GGTGGAGGTC AGAGTGCCAC GCTTGGCATC 
CAGGCATTTA CAGACAGTGC TGCCGAAGAA GTGGCTCTCA AGTGTTCCTG CCGACTCAAG 
GCTATGATCA TGTGCCAGGG CTGTGGCGCT TTCTGCCATG ATGATTGCAT CGGCCCTTCG 
AAACTGTGTG TGTCATGTCT GGTTGTCAGA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

asxl1 ( XM_021470008.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325683.1 Danio rerio additional sex combs like transcriptional regulator 1 (asxl1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa146304
Clone ID Related Accession (Same CDS sequence)	XM_021473722.1 , XM_021470008.1
Accession Version	XM_021470008.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	putative Polycomb group protein ASXL1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGACA AACAGAAGAG AAAGAAGGAG CGCACGTGGG CTGAGGCAGC TCGTATGGTT 
CTGGAGAACT TTTCTGATGC CCCAATGACG CCCAAACAGA TTCTACATGT CATCCAAACA 
AAGGGGTTGA AAGAAATGAG AAGCGGCACA GCACCACTGG CCTGCCTTGT CACCATGCTT 
CACTCTCAGG TCAGAGGTGA CCGAGTGAAA AACAGTATTT TCTTTAAGCT GCCTGGAAGG 
ATGAGTCTGT TTACCCTAAA GAAAAATGCA CTTCAGTGGA CAAAGAGCCC ATCAGCGAAT 
GAGTCTGGAG ATGGGACACC AACAGCATCC ACCACATCAA CAGTTGAAGG AGCAGAGCAG 
GAAAGCTGCG ACTCCACAGA GACGGCTGCT GCTAGTGGTG AAAATGATGC CTCTGTGGAT 
GAGACTTCCT CCAGTGCCTC TTGCTCTACA GAACCACAGA CAAGACTCAG TCGCTCTTCA 
CAGTCAGGGA GACAGAGGAA GAAGGCTGTG ATGATGCCCC GTGTAGTTCT CACTCCTCTC 
AAGGTCAACG GTGAACACGT CCCATCAGGA GCAGCGGGGA GGCGCAGGGA AGGCTCTAGG 
GGGGGTCCAG GCCCCACTCT CCGTACCCGC TCCGAGCTGG GCTGGAAACG CACCCAGCAC 
TTCAAGAGCA TTCGTGGTCT TCGTTCAGGT ACAGAGGATG AAAACCAAGG ACCCCCATGT 
AGTCCCATGA AAAGGAGTCG AGGAGGAGTG GAGGTGGATT TTGAGACTCC AGGATCCATC 
CTGGTCAACA CCAACATTCG TGCTCTCATC AACACACGGA CCTTTGCTGC TTTTCCTTCT 
CACTCCCAGC AGCAACTACT CCAGCTTTTA CCAGAGGTGG ACAGACAGGT CGGCCCCGAT 
GGCCTTGCTC GTCTAAGCAG TTCGGCCCTC AATAATGAAT TCTTCACTCA TGCCTCCCAG 
AGCTGGAAAG AACGTCTAGC TGAGGGTGAG TTTACACATG AGATGCAGGT TCGCTTCAGG 
CAGGAGATGG AAAAGGAGAA GAAAGTAGAA GCATGGAAGG AGAAATTCTT CGAGGAGTAC 
CATGGCCAAA GGTCTGGCTT AACAAGAGAG GAGGCATTGA AGCTCACAAT GAGCGAAGCT 
GGAGATGTTG CTAGTCCAGT GCTAGACTCC GATTCTGCTA CAGTGGCTAC ACCCAAGAGG 
CGAAGTGTTG GAAGAAGACG GAGAGAAGGC CGTATCAGAC GACGTTCACG GGCTGACCTC 
AGGCGCAGGG CAAGGCGCAC ACTTTGCAAA ACTACAACTG CAACTGTGGC TACAGAAGAA 
ACAACAGAGA GCACTGCAAC TCCAGAAGAG GTACCATCAG TTGCATCACC AGTCCCAGAA 
ACCACAGCAG TTCAAGGAGA GGTGGTCCTC CAAACAGACC CTGAACTTGA AGCACCAGAA 
GAAAACCTCT CTACAGAGGC AGTACCTTCT CCAGTTCCTG TACCCACACC TCCTCCAGCA 
TCGACAAGCT CAACCTGCGA TGAGCCAGAA ACCTCTACAC GCCTGTTGCC AGAAGTACCC 
GAACCAGCTG TTGCATCCAC CTCATCTCCT TCCTCATCCT CCTCTTCAAC ATCGTCATCA 
TCTTCACCAT CTTCCTCTCC ATCCTCTGCC TCAGACAGAC AGACTTTTGC TGCAAGCTCA 
GACTCCTCTT CTTCCTCTTC CTCCTCCACT GCTGCTGTTG CCACTGATCC GCTGGATGAT 
GGGGCTTCAG TCATTACCAC TGGCACAGCA GGCACTGGAG CAAGCAGCAG AGAAAGCAGC 
CCTGCAGCCA GTCCCGCTAC ATCAAGCCCA GCAATTCAGC TCAAGGAACA GAAGAGAAGA 
CCAGACGAGT CCCAGGCCTT CACCAGCTTT CCCGAGAAAA GGGCGCGGCT GGATGAGCGT 
CAGTCCTTTC GTAACACAGT TGACTGTGTG CACTCAGAAA AGCCACAGCC TACAACAGAG 
GAGCCCAAGG TCCCGCCAAT CCGGATACAG CTCTCCCGGA TCAAACCGCC CTGGGTCAAG 
GGGCCGCCAA CGTACCAAAT CTGTCCCCGC ATCGTCCCCC CCAACGAAGG GTCGCGGCGC 
TCTGGGACGG GGGCGCGCAC CTTGGCGGAC ATCAAAGCCC GTGCACAGCA AGCCCGTGCC 
CAGCGGGAAG CCGCTGCTGC TGTTGCAGCC TCTGGGGATG GGGGAGGGCC TGGGGGGAGC 
GGCCCGGGGG GTGGTACTGG GATACCGGAT CGCTCCAGCG GGAGGCGTAC AAGAGAGCAC 
CCAGGTCCCG TTGAACCTGG AGGAGGAGGA GGGAGACGAG GAGGAAGAGT TGATTTGGAG 
GAGCAGGAAT CGCCTGCGAG CTCTCATTCG TCGGGAGCAC AACTACAGCT GTCTAGTGTA 
GATTCGGCAG ACAGGCCTCA AACTTCCACC CCACTCACTG AACCATCTCC CTCTTCTGTG 
TCCTCAAACC CATCTCTACC ATCATCAGAA TCTCCAAAAA TACTTGCGCT ATCACCACCT 
GCAACCGACA GCCCTGCTTC CCAGGATCAG GTGGAGGAGA TTTGTGGAGT GGAAGAGGTG 
ACTGCCTGCC CAAGTGATAA AACCTCAGAG CAGACCTTAG ACACCCCAGT GCCTACCCCA 
AGTGAATCAG AGTCTTTTTG CAGTCACCAA GATGGGAGAG GCGAGGAAGC AGAGTCGAGT 
GATAGTAGAA CAAATACGCC TGTTTGCACC TCATCATCAA ATGATGCTAT TTCTTTAGTG 
CCCACCTCTA TACCTGACTC GCTACCAAGA TTTGGTGCCC AGGGTGTGGA TGTCATCAGG 
ACATTGGCAG CTTCCTCTCA GCCTTGGGAA GGTGAAAAGA AAGCGGTTGA GCTTCACCCA 
GGGACAACTG GAGTTATCCA GCATGGCTCA GACTTGAAAT TACCCAAAGA GACTCTTGTT 
ACAGCCAGAA ATGGATGGGT AGAAAATAGA GAAGAGCATT CCATGAGAGA GGGTATGCTA 
GAAGAACATA GGAATGGAGG GAGTGATGCT AATGGTAAAT ATGAATCAGG CTCTTTACCT 
TGCCTACCAC GATGTGCTGG TGAGGAAGAC ACTGGAGCAC ACAGTGATTC AACGGAGACA 
GCATCTGACT TTGAAAATGA ATCACAAGAG GATGAAATGG TGGATTGGCA TGGGACCCAG 
ATGGATGGTA GTGGCATGCA AACTCAAAAT ACAAAATCAC TGAATCGACC TGTTATTCAG 
ACCTCAAGTC GACTCACTTC ATCGATTGTT AATCCTCCTC AACACCAGCA ACCGGTTATA 
CAGGCCCATA TATCTAATCA AAACCATACC CAAACTGTTA TTCAGGCTTG CTTTCCTAAT 
GGTGTGCCAA ACCAACCAGT CATTCATCCT CACAAGCAGC ATGCAATTAA TACCCATGCT 
ATAAACCACT CTCAGGACCA GATTGCCTCT GCACCTTCAC AAGCCCAAGT GCAAGCTTAT 
CTGACCCAGA CTGAGATGGA CCAAAGCAGA AATCTACGAC TGAATGATAA TGGCAATGGT 
GTGAAGCTGT ATTTGTCAGA GGATGACACC AAACCTCTTG GCAGGGAAGA TTTAGTTTTA 
AAGAATTCTG CTGGTCCTCC AGTTGGCAGA AGAGTCCAAG TTTCATCTCG ACCAGTTTCC 
ACTGTAGAGG CCAACAATCC TCTTGTCACA CAGCTCCTCC AAGGCAGTCT ACCTTTAGAG 
AAGGTCTTAC CCCAACCCCA CTCAGCCAGC AAGTTAGAAA TTAATCGACT TCCAGGTGCC 
AATTCAAGCA ACTCGCTCAA CCCAGCCAAT CATCAAACAC AGAGAAATCT GGCACCACGT 
TTTAGAGGCC CACCTGAGTC GGGAGGGCCT ACGGACACTG TTAGTTCTGA CTTCCAACAC 
AAAACCCCAC CTGCTCGAGT ATCTCCTGGA CCTGGTCGGA ACTTTGGTTC TTCCCCGCCA 
GGTTCTGCTC CATCAAGAAT GGCATGCCTG TTTGAGGAAG CCAGCCCCAG GTCTGCAAAC 
TCTCAGTTCA GTTCTCAGCA ACCAGGAGGC GTTGTGCCTC CAGGAGCAGT ACCTGTCATA 
ACATCCCTTC CCTTAAACTC CACCACTAGA ACCTCAATGG ATATGAACTC TCAGAATGCT 
CCAGTTTATG AATCAGCTGT TATTAAAGAG CACCCTGGAC CTCTTTCTCA ACGGAGTGAA 
ACTCCTGAGA GGCCAAGTAG CTTTCAGCAG CATCCTAATA ATGTCTCCCA GTCCGTGTGC 
AGAAACACAC CAGATGCTCC TTCACCTCCA CATGGAGACC TTTGTCCATC AGAGGTTGTA 
CCAACTGTTA AGATTAACTG GCGCCCAAAC AAACCTCAGC AACCGATCTC CCCTGTAGCT 
ACAGTAAAAA ACGAAGTCTC CTTGCGCCCT TCGTGCCAAC AAGCTCTGAC CAAAAACTCA 
CCTGCTCCTA TCAGTGGCAA TACGTCTGTT GTAATAACCA AAAAGGAGCC TCTGGATGGT 
TTTCATGGAG GTGGTGGAGC TATGGAAGGA CTGCTAAATA TGGAAATGTC TCTTGTCAGA 
ATGGCCAAGA AGGAACAAGT CAAAAATCCA TATACCCGCC AAGCAGACAC CTCCGCTTCT 
CCTGTTTCTT CCTCTCCTTC CTCATCAGCC TCCAGTCTCC CTTTCCAGCT ATATGGTAAG 
CTACCAAAAC TTCAGCAGAG TGGGGGCAAC GGAGGGTCCT CATCCAGCTT CAGTTACACA 
GCTAATGTGT CTGTGGTGGA TGGTAGTGGC TTCACTCGCA CCTTAGCCGA TAGTGTGCTC 
CAACTAAGGC CACGTGTGAG CGTCGGCAGC GGTGGAGGTC AGAGTGCCAC GCTTGGCATC 
CAGGCATTTA CAGACAGTGC TGCCGAAGAA GTGGCTCTCA AGTGTTCCTG CCGACTCAAG 
GCTATGATCA TGTGCCAGGG CTGTGGCGCT TTCTGCCATG ATGATTGCAT CGGCCCTTCG 
AAACTGTGTG TGTCATGTCT GGTTGTCAGA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325683.1
CDS	483..5495
Translation	MKDKQKRKKERTWAEAARMVLENFSDAPMTPKQILHVIQTKGLKEMRSGTAPLACLVTMLHSQVRGDRVKNSIFFKLPGRMSLFTLKKNALQWTKSPSANESGDGTPTASTTSTVEGAEQESCDSTETAAASGENDASVDETSSSASCSTEPQTRLSRSSQSGRQRKKAVMMPRVVLTPLKVNGEHVPSGAAGRRREGSRGGPGPTLRTRSELGWKRTQHFKSIRGLRSGTEDENQGPPCSPMKRSRGGVEVDFETPGSILVNTNIRALINTRTFAAFPSHSQQQLLQLLPEVDRQVGPDGLARLSSSALNNEFFTHASQSWKERLAEGEFTHEMQVRFRQEMEKEKKVEAWKEKFFEEYHGQRSGLTREEALKLTMSEAGDVASPVLDSDSATVATPKRRSVGRRRREGRIRRRSRADLRRRARRTLCKTTTATVATEETTESTATPEEVPSVASPVPETTAVQGEVVLQTDPELEAPEENLSTEAVPSPVPVPTPPPASTSSTCDEPETSTRLLPEVPEPAVASTSSPSSSSSSTSSSSSPSSSPSSASDRQTFAASSDSSSSSSSSTAAVATDPLDDGASVITTGTAGTGASSRESSPAASPATSSPAIQLKEQKRRPDESQAFTSFPEKRARLDERQSFRNTVDCVHSEKPQPTTEEPKVPPIRIQLSRIKPPWVKGPPTYQICPRIVPPNEGSRRSGTGARTLADIKARAQQARAQREAAAAVAASGDGGGPGGSGPGGGTGIPDRSSGRRTREHPGPVEPGGGGGRRGGRVDLEEQESPASSHSSGAQLQLSSVDSADRPQTSTPLTEPSPSSVSSNPSLPSSESPKILALSPPATDSPASQDQVEEICGVEEVTACPSDKTSEQTLDTPVPTPSESESFCSHQDGRGEEAESSDSRTNTPVCTSSSNDAISLVPTSIPDSLPRFGAQGVDVIRTLAASSQPWEGEKKAVELHPGTTGVIQHGSDLKLPKETLVTARNGWVENREEHSMREGMLEEHRNGGSDANGKYESGSLPCLPRCAGEEDTGAHSDSTETASDFENESQEDEMVDWHGTQMDGSGMQTQNTKSLNRPVIQTSSRLTSSIVNPPQHQQPVIQAHISNQNHTQTVIQACFPNGVPNQPVIHPHKQHAINTHAINHSQDQIASAPSQAQVQAYLTQTEMDQSRNLRLNDNGNGVKLYLSEDDTKPLGREDLVLKNSAGPPVGRRVQVSSRPVSTVEANNPLVTQLLQGSLPLEKVLPQPHSASKLEINRLPGANSSNSLNPANHQTQRNLAPRFRGPPESGGPTDTVSSDFQHKTPPARVSPGPGRNFGSSPPGSAPSRMACLFEEASPRSANSQFSSQQPGGVVPPGAVPVITSLPLNSTTRTSMDMNSQNAPVYESAVIKEHPGPLSQRSETPERPSSFQQHPNNVSQSVCRNTPDAPSPPHGDLCPSEVVPTVKINWRPNKPQQPISPVATVKNEVSLRPSCQQALTKNSPAPISGNTSVVITKKEPLDGFHGGGGAMEGLLNMEMSLVRMAKKEQVKNPYTRQADTSASPVSSSPSSSASSLPFQLYGKLPKLQQSGGNGGSSSSFSYTANVSVVDGSGFTRTLADSVLQLRPRVSVGSGGGQSATLGIQAFTDSAAEEVALKCSCRLKAMIMCQGCGAFCHDDCIGPSKLCVSCLVVR

Target ORF information:

RefSeq Version	XM_021470008.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio additional sex combs like transcriptional regulator 1 (asxl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470008.1

ORF Insert Sequence:

ATGAAGGACA AACAGAAGAG AAAGAAGGAG CGCACGTGGG CTGAGGCAGC TCGTATGGTT 
CTGGAGAACT TTTCTGATGC CCCAATGACG CCCAAACAGA TTCTACATGT CATCCAAACA 
AAGGGGTTGA AAGAAATGAG AAGCGGCACA GCACCACTGG CCTGCCTTGT CACCATGCTT 
CACTCTCAGG TCAGAGGTGA CCGAGTGAAA AACAGTATTT TCTTTAAGCT GCCTGGAAGG 
ATGAGTCTGT TTACCCTAAA GAAAAATGCA CTTCAGTGGA CAAAGAGCCC ATCAGCGAAT 
GAGTCTGGAG ATGGGACACC AACAGCATCC ACCACATCAA CAGTTGAAGG AGCAGAGCAG 
GAAAGCTGCG ACTCCACAGA GACGGCTGCT GCTAGTGGTG AAAATGATGC CTCTGTGGAT 
GAGACTTCCT CCAGTGCCTC TTGCTCTACA GAACCACAGA CAAGACTCAG TCGCTCTTCA 
CAGTCAGGGA GACAGAGGAA GAAGGCTGTG ATGATGCCCC GTGTAGTTCT CACTCCTCTC 
AAGGTCAACG GTGAACACGT CCCATCAGGA GCAGCGGGGA GGCGCAGGGA AGGCTCTAGG 
GGGGGTCCAG GCCCCACTCT CCGTACCCGC TCCGAGCTGG GCTGGAAACG CACCCAGCAC 
TTCAAGAGCA TTCGTGGTCT TCGTTCAGGT ACAGAGGATG AAAACCAAGG ACCCCCATGT 
AGTCCCATGA AAAGGAGTCG AGGAGGAGTG GAGGTGGATT TTGAGACTCC AGGATCCATC 
CTGGTCAACA CCAACATTCG TGCTCTCATC AACACACGGA CCTTTGCTGC TTTTCCTTCT 
CACTCCCAGC AGCAACTACT CCAGCTTTTA CCAGAGGTGG ACAGACAGGT CGGCCCCGAT 
GGCCTTGCTC GTCTAAGCAG TTCGGCCCTC AATAATGAAT TCTTCACTCA TGCCTCCCAG 
AGCTGGAAAG AACGTCTAGC TGAGGGTGAG TTTACACATG AGATGCAGGT TCGCTTCAGG 
CAGGAGATGG AAAAGGAGAA GAAAGTAGAA GCATGGAAGG AGAAATTCTT CGAGGAGTAC 
CATGGCCAAA GGTCTGGCTT AACAAGAGAG GAGGCATTGA AGCTCACAAT GAGCGAAGCT 
GGAGATGTTG CTAGTCCAGT GCTAGACTCC GATTCTGCTA CAGTGGCTAC ACCCAAGAGG 
CGAAGTGTTG GAAGAAGACG GAGAGAAGGC CGTATCAGAC GACGTTCACG GGCTGACCTC 
AGGCGCAGGG CAAGGCGCAC ACTTTGCAAA ACTACAACTG CAACTGTGGC TACAGAAGAA 
ACAACAGAGA GCACTGCAAC TCCAGAAGAG GTACCATCAG TTGCATCACC AGTCCCAGAA 
ACCACAGCAG TTCAAGGAGA GGTGGTCCTC CAAACAGACC CTGAACTTGA AGCACCAGAA 
GAAAACCTCT CTACAGAGGC AGTACCTTCT CCAGTTCCTG TACCCACACC TCCTCCAGCA 
TCGACAAGCT CAACCTGCGA TGAGCCAGAA ACCTCTACAC GCCTGTTGCC AGAAGTACCC 
GAACCAGCTG TTGCATCCAC CTCATCTCCT TCCTCATCCT CCTCTTCAAC ATCGTCATCA 
TCTTCACCAT CTTCCTCTCC ATCCTCTGCC TCAGACAGAC AGACTTTTGC TGCAAGCTCA 
GACTCCTCTT CTTCCTCTTC CTCCTCCACT GCTGCTGTTG CCACTGATCC GCTGGATGAT 
GGGGCTTCAG TCATTACCAC TGGCACAGCA GGCACTGGAG CAAGCAGCAG AGAAAGCAGC 
CCTGCAGCCA GTCCCGCTAC ATCAAGCCCA GCAATTCAGC TCAAGGAACA GAAGAGAAGA 
CCAGACGAGT CCCAGGCCTT CACCAGCTTT CCCGAGAAAA GGGCGCGGCT GGATGAGCGT 
CAGTCCTTTC GTAACACAGT TGACTGTGTG CACTCAGAAA AGCCACAGCC TACAACAGAG 
GAGCCCAAGG TCCCGCCAAT CCGGATACAG CTCTCCCGGA TCAAACCGCC CTGGGTCAAG 
GGGCCGCCAA CGTACCAAAT CTGTCCCCGC ATCGTCCCCC CCAACGAAGG GTCGCGGCGC 
TCTGGGACGG GGGCGCGCAC CTTGGCGGAC ATCAAAGCCC GTGCACAGCA AGCCCGTGCC 
CAGCGGGAAG CCGCTGCTGC TGTTGCAGCC TCTGGGGATG GGGGAGGGCC TGGGGGGAGC 
GGCCCGGGGG GTGGTACTGG GATACCGGAT CGCTCCAGCG GGAGGCGTAC AAGAGAGCAC 
CCAGGTCCCG TTGAACCTGG AGGAGGAGGA GGGAGACGAG GAGGAAGAGT TGATTTGGAG 
GAGCAGGAAT CGCCTGCGAG CTCTCATTCG TCGGGAGCAC AACTACAGCT GTCTAGTGTA 
GATTCGGCAG ACAGGCCTCA AACTTCCACC CCACTCACTG AACCATCTCC CTCTTCTGTG 
TCCTCAAACC CATCTCTACC ATCATCAGAA TCTCCAAAAA TACTTGCGCT ATCACCACCT 
GCAACCGACA GCCCTGCTTC CCAGGATCAG GTGGAGGAGA TTTGTGGAGT GGAAGAGGTG 
ACTGCCTGCC CAAGTGATAA AACCTCAGAG CAGACCTTAG ACACCCCAGT GCCTACCCCA 
AGTGAATCAG AGTCTTTTTG CAGTCACCAA GATGGGAGAG GCGAGGAAGC AGAGTCGAGT 
GATAGTAGAA CAAATACGCC TGTTTGCACC TCATCATCAA ATGATGCTAT TTCTTTAGTG 
CCCACCTCTA TACCTGACTC GCTACCAAGA TTTGGTGCCC AGGGTGTGGA TGTCATCAGG 
ACATTGGCAG CTTCCTCTCA GCCTTGGGAA GGTGAAAAGA AAGCGGTTGA GCTTCACCCA 
GGGACAACTG GAGTTATCCA GCATGGCTCA GACTTGAAAT TACCCAAAGA GACTCTTGTT 
ACAGCCAGAA ATGGATGGGT AGAAAATAGA GAAGAGCATT CCATGAGAGA GGGTATGCTA 
GAAGAACATA GGAATGGAGG GAGTGATGCT AATGGTAAAT ATGAATCAGG CTCTTTACCT 
TGCCTACCAC GATGTGCTGG TGAGGAAGAC ACTGGAGCAC ACAGTGATTC AACGGAGACA 
GCATCTGACT TTGAAAATGA ATCACAAGAG GATGAAATGG TGGATTGGCA TGGGACCCAG 
ATGGATGGTA GTGGCATGCA AACTCAAAAT ACAAAATCAC TGAATCGACC TGTTATTCAG 
ACCTCAAGTC GACTCACTTC ATCGATTGTT AATCCTCCTC AACACCAGCA ACCGGTTATA 
CAGGCCCATA TATCTAATCA AAACCATACC CAAACTGTTA TTCAGGCTTG CTTTCCTAAT 
GGTGTGCCAA ACCAACCAGT CATTCATCCT CACAAGCAGC ATGCAATTAA TACCCATGCT 
ATAAACCACT CTCAGGACCA GATTGCCTCT GCACCTTCAC AAGCCCAAGT GCAAGCTTAT 
CTGACCCAGA CTGAGATGGA CCAAAGCAGA AATCTACGAC TGAATGATAA TGGCAATGGT 
GTGAAGCTGT ATTTGTCAGA GGATGACACC AAACCTCTTG GCAGGGAAGA TTTAGTTTTA 
AAGAATTCTG CTGGTCCTCC AGTTGGCAGA AGAGTCCAAG TTTCATCTCG ACCAGTTTCC 
ACTGTAGAGG CCAACAATCC TCTTGTCACA CAGCTCCTCC AAGGCAGTCT ACCTTTAGAG 
AAGGTCTTAC CCCAACCCCA CTCAGCCAGC AAGTTAGAAA TTAATCGACT TCCAGGTGCC 
AATTCAAGCA ACTCGCTCAA CCCAGCCAAT CATCAAACAC AGAGAAATCT GGCACCACGT 
TTTAGAGGCC CACCTGAGTC GGGAGGGCCT ACGGACACTG TTAGTTCTGA CTTCCAACAC 
AAAACCCCAC CTGCTCGAGT ATCTCCTGGA CCTGGTCGGA ACTTTGGTTC TTCCCCGCCA 
GGTTCTGCTC CATCAAGAAT GGCATGCCTG TTTGAGGAAG CCAGCCCCAG GTCTGCAAAC 
TCTCAGTTCA GTTCTCAGCA ACCAGGAGGC GTTGTGCCTC CAGGAGCAGT ACCTGTCATA 
ACATCCCTTC CCTTAAACTC CACCACTAGA ACCTCAATGG ATATGAACTC TCAGAATGCT 
CCAGTTTATG AATCAGCTGT TATTAAAGAG CACCCTGGAC CTCTTTCTCA ACGGAGTGAA 
ACTCCTGAGA GGCCAAGTAG CTTTCAGCAG CATCCTAATA ATGTCTCCCA GTCCGTGTGC 
AGAAACACAC CAGATGCTCC TTCACCTCCA CATGGAGACC TTTGTCCATC AGAGGTTGTA 
CCAACTGTTA AGATTAACTG GCGCCCAAAC AAACCTCAGC AACCGATCTC CCCTGTAGCT 
ACAGTAAAAA ACGAAGTCTC CTTGCGCCCT TCGTGCCAAC AAGCTCTGAC CAAAAACTCA 
CCTGCTCCTA TCAGTGGCAA TACGTCTGTT GTAATAACCA AAAAGGAGCC TCTGGATGGT 
TTTCATGGAG GTGGTGGAGC TATGGAAGGA CTGCTAAATA TGGAAATGTC TCTTGTCAGA 
ATGGCCAAGA AGGAACAAGT CAAAAATCCA TATACCCGCC AAGCAGACAC CTCCGCTTCT 
CCTGTTTCTT CCTCTCCTTC CTCATCAGCC TCCAGTCTCC CTTTCCAGCT ATATGGTAAG 
CTACCAAAAC TTCAGCAGAG TGGGGGCAAC GGAGGGTCCT CATCCAGCTT CAGTTACACA 
GCTAATGTGT CTGTGGTGGA TGGTAGTGGC TTCACTCGCA CCTTAGCCGA TAGTGTGCTC 
CAACTAAGGC CACGTGTGAG CGTCGGCAGC GGTGGAGGTC AGAGTGCCAC GCTTGGCATC 
CAGGCATTTA CAGACAGTGC TGCCGAAGAA GTGGCTCTCA AGTGTTCCTG CCGACTCAAG 
GCTATGATCA TGTGCCAGGG CTGTGGCGCT TTCTGCCATG ATGATTGCAT CGGCCCTTCG 
AAACTGTGTG TGTCATGTCT GGTTGTCAGA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

asxl1 ( XM_021473723.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329398.1 Danio rerio additional sex combs like transcriptional regulator 1 (asxl1), transcript variant X2, mRNA.

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CloneID	ODa27915
Clone ID Related Accession (Same CDS sequence)	XM_021473723.1 , XM_005162338.1
Accession Version	XM_021473723.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	putative Polycomb group protein ASXL1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395158.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGACA AACAGAAGAG AAAGAAGGAG CGCACGTGGG CTGAGGCAGC TCGTATGGTT 
CTGGAGAACT TTTCTGATGC CCCAATGACG CCCAAACAGA TTCTACATGT CATCCAAACA 
AAGGGGTTGA AAGAAATGAG AAGCGGCACA GCACCACTGG CCTGCCTTGT CACCATGCTT 
CACTCTCAGG TCAGAGGTGA CCGAGTGAAA AACAGTATTT TCTTTAAGCT GCCTGGAAGG 
ATGAGTCTGT TTACCCTAAA GAAAAATGCA CTTCAGTGGA CAAAGAGCCC ATCAGCGAAT 
GAGTCTGGAG ATGGGACACC AACAGCATCC ACCACATCAA CAGTTGAAGG AGCAGAGCAG 
GAAAGCTGCG ACTCCACAGA GACGGCTGCT GCTAGTGGTG AAAATGATGC CTCTGTGGAT 
GAGACTTCCT CCAGTGCCTC TTGCTCTACA GAACCACAGA CAAGACTCAG TCGCTCTTCA 
CAGTCAGGGA GACAGAGGAA GAAGGCTGTG ATGATGCCCC GTGTAGTTCT CACTCCTCTC 
AAGGTCAACG GTGAACACGT CCCATCAGGA GCAGCGGGGA GGCGCAGGGA AGGCTCTAGG 
GGGGGTCCAG GCCCCACTCT CCGTACCCGC TCCGAGCTGG GCTGGAAACG CACCCAGCAC 
TTCAAGAGCA TTCGTGGTCT TCGTTCAGGT CCCATGAAAA GGAGTCGAGG AGGAGTGGAG 
GTGGATTTTG AGACTCCAGG ATCCATCCTG GTCAACACCA ACATTCGTGC TCTCATCAAC 
ACACGGACCT TTGCTGCTTT TCCTTCTCAC TCCCAGCAGC AACTACTCCA GCTTTTACCA 
GAGGTGGACA GACAGGTCGG CCCCGATGGC CTTGCTCGTC TAAGCAGTTC GGCCCTCAAT 
AATGAATTCT TCACTCATGC CTCCCAGAGC TGGAAAGAAC GTCTAGCTGA GGGTGAGTTT 
ACACATGAGA TGCAGGTTCG CTTCAGGCAG GAGATGGAAA AGGAGAAGAA AGTAGAAGCA 
TGGAAGGAGA AATTCTTCGA GGAGTACCAT GGCCAAAGGT CTGGCTTAAC AAGAGAGGAG 
GCATTGAAGC TCACAATGAG CGAAGCTGGA GATGTTGCTA GTCCAGTGCT AGACTCCGAT 
TCTGCTACAG TGGCTACACC CAAGAGGCGA AGTGTTGGAA GAAGACGGAG AGAAGGCCGT 
ATCAGACGAC GTTCACGGGC TGACCTCAGG CGCAGGGCAA GGCGCACACT TTGCAAAACT 
ACAACTGCAA CTGTGGCTAC AGAAGAAACA ACAGAGAGCA CTGCAACTCC AGAAGAGGTA 
CCATCAGTTG CATCACCAGT CCCAGAAACC ACAGCAGTTC AAGGAGAGGT GGTCCTCCAA 
ACAGACCCTG AACTTGAAGC ACCAGAAGAA AACCTCTCTA CAGAGGCAGT ACCTTCTCCA 
GTTCCTGTAC CCACACCTCC TCCAGCATCG ACAAGCTCAA CCTGCGATGA GCCAGAAACC 
TCTACACGCC TGTTGCCAGA AGTACCCGAA CCAGCTGTTG CATCCACCTC ATCTCCTTCC 
TCATCCTCCT CTTCAACATC GTCATCATCT TCACCATCTT CCTCTCCATC CTCTGCCTCA 
GACAGACAGA CTTTTGCTGC AAGCTCAGAC TCCTCTTCTT CCTCTTCCTC CTCCACTGCT 
GCTGTTGCCA CTGATCCGCT GGATGATGGG GCTTCAGTCA TTACCACTGG CACAGCAGGC 
ACTGGAGCAA GCAGCAGAGA AAGCAGCCCT GCAGCCAGTC CCGCTACATC AAGCCCAGCA 
ATTCAGCTCA AGGAACAGAA GAGAAGACCA GACGAGTCCC AGGCCTTCAC CAGCTTTCCC 
GAGAAAAGGG CGCGGCTGGA TGAGCGTCAG TCCTTTCGTA ACACAGTTGA CTGTGTGCAC 
TCAGAAAAGC CACAGCCTAC AACAGAGGAG CCCAAGGTCC CGCCAATCCG GATACAGCTC 
TCCCGGATCA AACCGCCCTG GGTCAAGGGG CCGCCAACGT ACCAAATCTG TCCCCGCATC 
GTCCCCCCCA ACGAAGGGTC GCGGCGCTCT GGGACGGGGG CGCGCACCTT GGCGGACATC 
AAAGCCCGTG CACAGCAAGC CCGTGCCCAG CGGGAAGCCG CTGCTGCTGT TGCAGCCTCT 
GGGGATGGGG GAGGGCCTGG GGGGAGCGGC CCGGGGGGTG GTACTGGGAT ACCGGATCGC 
TCCAGCGGGA GGCGTACAAG AGAGCACCCA GGTCCCGTTG AACCTGGAGG AGGAGGAGGG 
AGACGAGGAG GAAGAGTTGA TTTGGAGGAG CAGGAATCGC CTGCGAGCTC TCATTCGTCG 
GGAGCACAAC TACAGCTGTC TAGTGTAGAT TCGGCAGACA GGCCTCAAAC TTCCACCCCA 
CTCACTGAAC CATCTCCCTC TTCTGTGTCC TCAAACCCAT CTCTACCATC ATCAGAATCT 
CCAAAAATAC TTGCGCTATC ACCACCTGCA ACCGACAGCC CTGCTTCCCA GGATCAGGTG 
GAGGAGATTT GTGGAGTGGA AGAGGTGACT GCCTGCCCAA GTGATAAAAC CTCAGAGCAG 
ACCTTAGACA CCCCAGTGCC TACCCCAAGT GAATCAGAGT CTTTTTGCAG TCACCAAGAT 
GGGAGAGGCG AGGAAGCAGA GTCGAGTGAT AGTAGAACAA ATACGCCTGT TTGCACCTCA 
TCATCAAATG ATGCTATTTC TTTAGTGCCC ACCTCTATAC CTGACTCGCT ACCAAGATTT 
GGTGCCCAGG GTGTGGATGT CATCAGGACA TTGGCAGCTT CCTCTCAGCC TTGGGAAGGT 
GAAAAGAAAG CGGTTGAGCT TCACCCAGGG ACAACTGGAG TTATCCAGCA TGGCTCAGAC 
TTGAAATTAC CCAAAGAGAC TCTTGTTACA GCCAGAAATG GATGGGTAGA AAATAGAGAA 
GAGCATTCCA TGAGAGAGGG TATGCTAGAA GAACATAGGA ATGGAGGGAG TGATGCTAAT 
GGTAAATATG AATCAGGCTC TTTACCTTGC CTACCACGAT GTGCTGGTGA GGAAGACACT 
GGAGCACACA GTGATTCAAC GGAGACAGCA TCTGACTTTG AAAATGAATC ACAAGAGGAT 
GAAATGGTGG ATTGGCATGG GACCCAGATG GATGGTAGTG GCATGCAAAC TCAAAATACA 
AAATCACTGA ATCGACCTGT TATTCAGACC TCAAGTCGAC TCACTTCATC GATTGTTAAT 
CCTCCTCAAC ACCAGCAACC GGTTATACAG GCCCATATAT CTAATCAAAA CCATACCCAA 
ACTGTTATTC AGGCTTGCTT TCCTAATGGT GTGCCAAACC AACCAGTCAT TCATCCTCAC 
AAGCAGCATG CAATTAATAC CCATGCTATA AACCACTCTC AGGACCAGAT TGCCTCTGCA 
CCTTCACAAG CCCAAGTGCA AGCTTATCTG ACCCAGACTG AGATGGACCA AAGCAGAAAT 
CTACGACTGA ATGATAATGG CAATGGTGTG AAGCTGTATT TGTCAGAGGA TGACACCAAA 
CCTCTTGGCA GGGAAGATTT AGTTTTAAAG AATTCTGCTG GTCCTCCAGT TGGCAGAAGA 
GTCCAAGTTT CATCTCGACC AGTTTCCACT GTAGAGGCCA ACAATCCTCT TGTCACACAG 
CTCCTCCAAG GCAGTCTACC TTTAGAGAAG GTCTTACCCC AACCCCACTC AGCCAGCAAG 
TTAGAAATTA ATCGACTTCC AGGTGCCAAT TCAAGCAACT CGCTCAACCC AGCCAATCAT 
CAAACACAGA GAAATCTGGC ACCACGTTTT AGAGGCCCAC CTGAGTCGGG AGGGCCTACG 
GACACTGTTA GTTCTGACTT CCAACACAAA ACCCCACCTG CTCGAGTATC TCCTGGACCT 
GGTCGGAACT TTGGTTCTTC CCCGCCAGGT TCTGCTCCAT CAAGAATGGC ATGCCTGTTT 
GAGGAAGCCA GCCCCAGGTC TGCAAACTCT CAGTTCAGTT CTCAGCAACC AGGAGGCGTT 
GTGCCTCCAG GAGCAGTACC TGTCATAACA TCCCTTCCCT TAAACTCCAC CACTAGAACC 
TCAATGGATA TGAACTCTCA GAATGCTCCA GTTTATGAAT CAGCTGTTAT TAAAGAGCAC 
CCTGGACCTC TTTCTCAACG GAGTGAAACT CCTGAGAGGC CAAGTAGCTT TCAGCAGCAT 
CCTAATAATG TCTCCCAGTC CGTGTGCAGA AACACACCAG ATGCTCCTTC ACCTCCACAT 
GGAGACCTTT GTCCATCAGA GGTTGTACCA ACTGTTAAGA TTAACTGGCG CCCAAACAAA 
CCTCAGCAAC CGATCTCCCC TGTAGCTACA GTAAAAAACG AAGTCTCCTT GCGCCCTTCG 
TGCCAACAAG CTCTGACCAA AAACTCACCT GCTCCTATCA GTGGCAATAC GTCTGTTGTA 
ATAACCAAAA AGGAGCCTCT GGATGGTTTT CATGGAGGTG GTGGAGCTAT GGAAGGACTG 
CTAAATATGG AAATGTCTCT TGTCAGAATG GCCAAGAAGG AACAAGTCAA AAATCCATAT 
ACCCGCCAAG CAGACACCTC CGCTTCTCCT GTTTCTTCCT CTCCTTCCTC ATCAGCCTCC 
AGTCTCCCTT TCCAGCTATA TGGTAAGCTA CCAAAACTTC AGCAGAGTGG GGGCAACGGA 
GGGTCCTCAT CCAGCTTCAG TTACACAGCT AATGTGTCTG TGGTGGATGG TAGTGGCTTC 
ACTCGCACCT TAGCCGATAG TGTGCTCCAA CTAAGGCCAC GTGTGAGCGT CGGCAGCGGT 
GGAGGTCAGA GTGCCACGCT TGGCATCCAG GCATTTACAG ACAGTGCTGC CGAAGAAGTG 
GCTCTCAAGT GTTCCTGCCG ACTCAAGGCT ATGATCATGT GCCAGGGCTG TGGCGCTTTC 
TGCCATGATG ATTGCATCGG CCCTTCGAAA CTGTGTGTGT CATGTCTGGT TGTCAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329398.1
CDS	482..5461
Translation	MKDKQKRKKERTWAEAARMVLENFSDAPMTPKQILHVIQTKGLKEMRSGTAPLACLVTMLHSQVRGDRVKNSIFFKLPGRMSLFTLKKNALQWTKSPSANESGDGTPTASTTSTVEGAEQESCDSTETAAASGENDASVDETSSSASCSTEPQTRLSRSSQSGRQRKKAVMMPRVVLTPLKVNGEHVPSGAAGRRREGSRGGPGPTLRTRSELGWKRTQHFKSIRGLRSGPMKRSRGGVEVDFETPGSILVNTNIRALINTRTFAAFPSHSQQQLLQLLPEVDRQVGPDGLARLSSSALNNEFFTHASQSWKERLAEGEFTHEMQVRFRQEMEKEKKVEAWKEKFFEEYHGQRSGLTREEALKLTMSEAGDVASPVLDSDSATVATPKRRSVGRRRREGRIRRRSRADLRRRARRTLCKTTTATVATEETTESTATPEEVPSVASPVPETTAVQGEVVLQTDPELEAPEENLSTEAVPSPVPVPTPPPASTSSTCDEPETSTRLLPEVPEPAVASTSSPSSSSSSTSSSSSPSSSPSSASDRQTFAASSDSSSSSSSSTAAVATDPLDDGASVITTGTAGTGASSRESSPAASPATSSPAIQLKEQKRRPDESQAFTSFPEKRARLDERQSFRNTVDCVHSEKPQPTTEEPKVPPIRIQLSRIKPPWVKGPPTYQICPRIVPPNEGSRRSGTGARTLADIKARAQQARAQREAAAAVAASGDGGGPGGSGPGGGTGIPDRSSGRRTREHPGPVEPGGGGGRRGGRVDLEEQESPASSHSSGAQLQLSSVDSADRPQTSTPLTEPSPSSVSSNPSLPSSESPKILALSPPATDSPASQDQVEEICGVEEVTACPSDKTSEQTLDTPVPTPSESESFCSHQDGRGEEAESSDSRTNTPVCTSSSNDAISLVPTSIPDSLPRFGAQGVDVIRTLAASSQPWEGEKKAVELHPGTTGVIQHGSDLKLPKETLVTARNGWVENREEHSMREGMLEEHRNGGSDANGKYESGSLPCLPRCAGEEDTGAHSDSTETASDFENESQEDEMVDWHGTQMDGSGMQTQNTKSLNRPVIQTSSRLTSSIVNPPQHQQPVIQAHISNQNHTQTVIQACFPNGVPNQPVIHPHKQHAINTHAINHSQDQIASAPSQAQVQAYLTQTEMDQSRNLRLNDNGNGVKLYLSEDDTKPLGREDLVLKNSAGPPVGRRVQVSSRPVSTVEANNPLVTQLLQGSLPLEKVLPQPHSASKLEINRLPGANSSNSLNPANHQTQRNLAPRFRGPPESGGPTDTVSSDFQHKTPPARVSPGPGRNFGSSPPGSAPSRMACLFEEASPRSANSQFSSQQPGGVVPPGAVPVITSLPLNSTTRTSMDMNSQNAPVYESAVIKEHPGPLSQRSETPERPSSFQQHPNNVSQSVCRNTPDAPSPPHGDLCPSEVVPTVKINWRPNKPQQPISPVATVKNEVSLRPSCQQALTKNSPAPISGNTSVVITKKEPLDGFHGGGGAMEGLLNMEMSLVRMAKKEQVKNPYTRQADTSASPVSSSPSSSASSLPFQLYGKLPKLQQSGGNGGSSSSFSYTANVSVVDGSGFTRTLADSVLQLRPRVSVGSGGGQSATLGIQAFTDSAAEEVALKCSCRLKAMIMCQGCGAFCHDDCIGPSKLCVSCLVVR

Target ORF information:

RefSeq Version	XM_021473723.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio additional sex combs like transcriptional regulator 1 (asxl1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473723.1

ORF Insert Sequence:

ATGAAGGACA AACAGAAGAG AAAGAAGGAG CGCACGTGGG CTGAGGCAGC TCGTATGGTT 
CTGGAGAACT TTTCTGATGC CCCAATGACG CCCAAACAGA TTCTACATGT CATCCAAACA 
AAGGGGTTGA AAGAAATGAG AAGCGGCACA GCACCACTGG CCTGCCTTGT CACCATGCTT 
CACTCTCAGG TCAGAGGTGA CCGAGTGAAA AACAGTATTT TCTTTAAGCT GCCTGGAAGG 
ATGAGTCTGT TTACCCTAAA GAAAAATGCA CTTCAGTGGA CAAAGAGCCC ATCAGCGAAT 
GAGTCTGGAG ATGGGACACC AACAGCATCC ACCACATCAA CAGTTGAAGG AGCAGAGCAG 
GAAAGCTGCG ACTCCACAGA GACGGCTGCT GCTAGTGGTG AAAATGATGC CTCTGTGGAT 
GAGACTTCCT CCAGTGCCTC TTGCTCTACA GAACCACAGA CAAGACTCAG TCGCTCTTCA 
CAGTCAGGGA GACAGAGGAA GAAGGCTGTG ATGATGCCCC GTGTAGTTCT CACTCCTCTC 
AAGGTCAACG GTGAACACGT CCCATCAGGA GCAGCGGGGA GGCGCAGGGA AGGCTCTAGG 
GGGGGTCCAG GCCCCACTCT CCGTACCCGC TCCGAGCTGG GCTGGAAACG CACCCAGCAC 
TTCAAGAGCA TTCGTGGTCT TCGTTCAGGT CCCATGAAAA GGAGTCGAGG AGGAGTGGAG 
GTGGATTTTG AGACTCCAGG ATCCATCCTG GTCAACACCA ACATTCGTGC TCTCATCAAC 
ACACGGACCT TTGCTGCTTT TCCTTCTCAC TCCCAGCAGC AACTACTCCA GCTTTTACCA 
GAGGTGGACA GACAGGTCGG CCCCGATGGC CTTGCTCGTC TAAGCAGTTC GGCCCTCAAT 
AATGAATTCT TCACTCATGC CTCCCAGAGC TGGAAAGAAC GTCTAGCTGA GGGTGAGTTT 
ACACATGAGA TGCAGGTTCG CTTCAGGCAG GAGATGGAAA AGGAGAAGAA AGTAGAAGCA 
TGGAAGGAGA AATTCTTCGA GGAGTACCAT GGCCAAAGGT CTGGCTTAAC AAGAGAGGAG 
GCATTGAAGC TCACAATGAG CGAAGCTGGA GATGTTGCTA GTCCAGTGCT AGACTCCGAT 
TCTGCTACAG TGGCTACACC CAAGAGGCGA AGTGTTGGAA GAAGACGGAG AGAAGGCCGT 
ATCAGACGAC GTTCACGGGC TGACCTCAGG CGCAGGGCAA GGCGCACACT TTGCAAAACT 
ACAACTGCAA CTGTGGCTAC AGAAGAAACA ACAGAGAGCA CTGCAACTCC AGAAGAGGTA 
CCATCAGTTG CATCACCAGT CCCAGAAACC ACAGCAGTTC AAGGAGAGGT GGTCCTCCAA 
ACAGACCCTG AACTTGAAGC ACCAGAAGAA AACCTCTCTA CAGAGGCAGT ACCTTCTCCA 
GTTCCTGTAC CCACACCTCC TCCAGCATCG ACAAGCTCAA CCTGCGATGA GCCAGAAACC 
TCTACACGCC TGTTGCCAGA AGTACCCGAA CCAGCTGTTG CATCCACCTC ATCTCCTTCC 
TCATCCTCCT CTTCAACATC GTCATCATCT TCACCATCTT CCTCTCCATC CTCTGCCTCA 
GACAGACAGA CTTTTGCTGC AAGCTCAGAC TCCTCTTCTT CCTCTTCCTC CTCCACTGCT 
GCTGTTGCCA CTGATCCGCT GGATGATGGG GCTTCAGTCA TTACCACTGG CACAGCAGGC 
ACTGGAGCAA GCAGCAGAGA AAGCAGCCCT GCAGCCAGTC CCGCTACATC AAGCCCAGCA 
ATTCAGCTCA AGGAACAGAA GAGAAGACCA GACGAGTCCC AGGCCTTCAC CAGCTTTCCC 
GAGAAAAGGG CGCGGCTGGA TGAGCGTCAG TCCTTTCGTA ACACAGTTGA CTGTGTGCAC 
TCAGAAAAGC CACAGCCTAC AACAGAGGAG CCCAAGGTCC CGCCAATCCG GATACAGCTC 
TCCCGGATCA AACCGCCCTG GGTCAAGGGG CCGCCAACGT ACCAAATCTG TCCCCGCATC 
GTCCCCCCCA ACGAAGGGTC GCGGCGCTCT GGGACGGGGG CGCGCACCTT GGCGGACATC 
AAAGCCCGTG CACAGCAAGC CCGTGCCCAG CGGGAAGCCG CTGCTGCTGT TGCAGCCTCT 
GGGGATGGGG GAGGGCCTGG GGGGAGCGGC CCGGGGGGTG GTACTGGGAT ACCGGATCGC 
TCCAGCGGGA GGCGTACAAG AGAGCACCCA GGTCCCGTTG AACCTGGAGG AGGAGGAGGG 
AGACGAGGAG GAAGAGTTGA TTTGGAGGAG CAGGAATCGC CTGCGAGCTC TCATTCGTCG 
GGAGCACAAC TACAGCTGTC TAGTGTAGAT TCGGCAGACA GGCCTCAAAC TTCCACCCCA 
CTCACTGAAC CATCTCCCTC TTCTGTGTCC TCAAACCCAT CTCTACCATC ATCAGAATCT 
CCAAAAATAC TTGCGCTATC ACCACCTGCA ACCGACAGCC CTGCTTCCCA GGATCAGGTG 
GAGGAGATTT GTGGAGTGGA AGAGGTGACT GCCTGCCCAA GTGATAAAAC CTCAGAGCAG 
ACCTTAGACA CCCCAGTGCC TACCCCAAGT GAATCAGAGT CTTTTTGCAG TCACCAAGAT 
GGGAGAGGCG AGGAAGCAGA GTCGAGTGAT AGTAGAACAA ATACGCCTGT TTGCACCTCA 
TCATCAAATG ATGCTATTTC TTTAGTGCCC ACCTCTATAC CTGACTCGCT ACCAAGATTT 
GGTGCCCAGG GTGTGGATGT CATCAGGACA TTGGCAGCTT CCTCTCAGCC TTGGGAAGGT 
GAAAAGAAAG CGGTTGAGCT TCACCCAGGG ACAACTGGAG TTATCCAGCA TGGCTCAGAC 
TTGAAATTAC CCAAAGAGAC TCTTGTTACA GCCAGAAATG GATGGGTAGA AAATAGAGAA 
GAGCATTCCA TGAGAGAGGG TATGCTAGAA GAACATAGGA ATGGAGGGAG TGATGCTAAT 
GGTAAATATG AATCAGGCTC TTTACCTTGC CTACCACGAT GTGCTGGTGA GGAAGACACT 
GGAGCACACA GTGATTCAAC GGAGACAGCA TCTGACTTTG AAAATGAATC ACAAGAGGAT 
GAAATGGTGG ATTGGCATGG GACCCAGATG GATGGTAGTG GCATGCAAAC TCAAAATACA 
AAATCACTGA ATCGACCTGT TATTCAGACC TCAAGTCGAC TCACTTCATC GATTGTTAAT 
CCTCCTCAAC ACCAGCAACC GGTTATACAG GCCCATATAT CTAATCAAAA CCATACCCAA 
ACTGTTATTC AGGCTTGCTT TCCTAATGGT GTGCCAAACC AACCAGTCAT TCATCCTCAC 
AAGCAGCATG CAATTAATAC CCATGCTATA AACCACTCTC AGGACCAGAT TGCCTCTGCA 
CCTTCACAAG CCCAAGTGCA AGCTTATCTG ACCCAGACTG AGATGGACCA AAGCAGAAAT 
CTACGACTGA ATGATAATGG CAATGGTGTG AAGCTGTATT TGTCAGAGGA TGACACCAAA 
CCTCTTGGCA GGGAAGATTT AGTTTTAAAG AATTCTGCTG GTCCTCCAGT TGGCAGAAGA 
GTCCAAGTTT CATCTCGACC AGTTTCCACT GTAGAGGCCA ACAATCCTCT TGTCACACAG 
CTCCTCCAAG GCAGTCTACC TTTAGAGAAG GTCTTACCCC AACCCCACTC AGCCAGCAAG 
TTAGAAATTA ATCGACTTCC AGGTGCCAAT TCAAGCAACT CGCTCAACCC AGCCAATCAT 
CAAACACAGA GAAATCTGGC ACCACGTTTT AGAGGCCCAC CTGAGTCGGG AGGGCCTACG 
GACACTGTTA GTTCTGACTT CCAACACAAA ACCCCACCTG CTCGAGTATC TCCTGGACCT 
GGTCGGAACT TTGGTTCTTC CCCGCCAGGT TCTGCTCCAT CAAGAATGGC ATGCCTGTTT 
GAGGAAGCCA GCCCCAGGTC TGCAAACTCT CAGTTCAGTT CTCAGCAACC AGGAGGCGTT 
GTGCCTCCAG GAGCAGTACC TGTCATAACA TCCCTTCCCT TAAACTCCAC CACTAGAACC 
TCAATGGATA TGAACTCTCA GAATGCTCCA GTTTATGAAT CAGCTGTTAT TAAAGAGCAC 
CCTGGACCTC TTTCTCAACG GAGTGAAACT CCTGAGAGGC CAAGTAGCTT TCAGCAGCAT 
CCTAATAATG TCTCCCAGTC CGTGTGCAGA AACACACCAG ATGCTCCTTC ACCTCCACAT 
GGAGACCTTT GTCCATCAGA GGTTGTACCA ACTGTTAAGA TTAACTGGCG CCCAAACAAA 
CCTCAGCAAC CGATCTCCCC TGTAGCTACA GTAAAAAACG AAGTCTCCTT GCGCCCTTCG 
TGCCAACAAG CTCTGACCAA AAACTCACCT GCTCCTATCA GTGGCAATAC GTCTGTTGTA 
ATAACCAAAA AGGAGCCTCT GGATGGTTTT CATGGAGGTG GTGGAGCTAT GGAAGGACTG 
CTAAATATGG AAATGTCTCT TGTCAGAATG GCCAAGAAGG AACAAGTCAA AAATCCATAT 
ACCCGCCAAG CAGACACCTC CGCTTCTCCT GTTTCTTCCT CTCCTTCCTC ATCAGCCTCC 
AGTCTCCCTT TCCAGCTATA TGGTAAGCTA CCAAAACTTC AGCAGAGTGG GGGCAACGGA 
GGGTCCTCAT CCAGCTTCAG TTACACAGCT AATGTGTCTG TGGTGGATGG TAGTGGCTTC 
ACTCGCACCT TAGCCGATAG TGTGCTCCAA CTAAGGCCAC GTGTGAGCGT CGGCAGCGGT 
GGAGGTCAGA GTGCCACGCT TGGCATCCAG GCATTTACAG ACAGTGCTGC CGAAGAAGTG 
GCTCTCAAGT GTTCCTGCCG ACTCAAGGCT ATGATCATGT GCCAGGGCTG TGGCGCTTTC 
TGCCATGATG ATTGCATCGG CCCTTCGAAA CTGTGTGTGT CATGTCTGGT TGTCAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

asxl1 ( XM_005162338.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162395.1 Danio rerio additional sex combs like transcriptional regulator 1 (asxl1), transcript variant X2, mRNA.

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CloneID	ODa27915
Clone ID Related Accession (Same CDS sequence)	XM_021473723.1 , XM_005162338.1
Accession Version	XM_005162338.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	putative Polycomb group protein ASXL1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGACA AACAGAAGAG AAAGAAGGAG CGCACGTGGG CTGAGGCAGC TCGTATGGTT 
CTGGAGAACT TTTCTGATGC CCCAATGACG CCCAAACAGA TTCTACATGT CATCCAAACA 
AAGGGGTTGA AAGAAATGAG AAGCGGCACA GCACCACTGG CCTGCCTTGT CACCATGCTT 
CACTCTCAGG TCAGAGGTGA CCGAGTGAAA AACAGTATTT TCTTTAAGCT GCCTGGAAGG 
ATGAGTCTGT TTACCCTAAA GAAAAATGCA CTTCAGTGGA CAAAGAGCCC ATCAGCGAAT 
GAGTCTGGAG ATGGGACACC AACAGCATCC ACCACATCAA CAGTTGAAGG AGCAGAGCAG 
GAAAGCTGCG ACTCCACAGA GACGGCTGCT GCTAGTGGTG AAAATGATGC CTCTGTGGAT 
GAGACTTCCT CCAGTGCCTC TTGCTCTACA GAACCACAGA CAAGACTCAG TCGCTCTTCA 
CAGTCAGGGA GACAGAGGAA GAAGGCTGTG ATGATGCCCC GTGTAGTTCT CACTCCTCTC 
AAGGTCAACG GTGAACACGT CCCATCAGGA GCAGCGGGGA GGCGCAGGGA AGGCTCTAGG 
GGGGGTCCAG GCCCCACTCT CCGTACCCGC TCCGAGCTGG GCTGGAAACG CACCCAGCAC 
TTCAAGAGCA TTCGTGGTCT TCGTTCAGGT CCCATGAAAA GGAGTCGAGG AGGAGTGGAG 
GTGGATTTTG AGACTCCAGG ATCCATCCTG GTCAACACCA ACATTCGTGC TCTCATCAAC 
ACACGGACCT TTGCTGCTTT TCCTTCTCAC TCCCAGCAGC AACTACTCCA GCTTTTACCA 
GAGGTGGACA GACAGGTCGG CCCCGATGGC CTTGCTCGTC TAAGCAGTTC GGCCCTCAAT 
AATGAATTCT TCACTCATGC CTCCCAGAGC TGGAAAGAAC GTCTAGCTGA GGGTGAGTTT 
ACACATGAGA TGCAGGTTCG CTTCAGGCAG GAGATGGAAA AGGAGAAGAA AGTAGAAGCA 
TGGAAGGAGA AATTCTTCGA GGAGTACCAT GGCCAAAGGT CTGGCTTAAC AAGAGAGGAG 
GCATTGAAGC TCACAATGAG CGAAGCTGGA GATGTTGCTA GTCCAGTGCT AGACTCCGAT 
TCTGCTACAG TGGCTACACC CAAGAGGCGA AGTGTTGGAA GAAGACGGAG AGAAGGCCGT 
ATCAGACGAC GTTCACGGGC TGACCTCAGG CGCAGGGCAA GGCGCACACT TTGCAAAACT 
ACAACTGCAA CTGTGGCTAC AGAAGAAACA ACAGAGAGCA CTGCAACTCC AGAAGAGGTA 
CCATCAGTTG CATCACCAGT CCCAGAAACC ACAGCAGTTC AAGGAGAGGT GGTCCTCCAA 
ACAGACCCTG AACTTGAAGC ACCAGAAGAA AACCTCTCTA CAGAGGCAGT ACCTTCTCCA 
GTTCCTGTAC CCACACCTCC TCCAGCATCG ACAAGCTCAA CCTGCGATGA GCCAGAAACC 
TCTACACGCC TGTTGCCAGA AGTACCCGAA CCAGCTGTTG CATCCACCTC ATCTCCTTCC 
TCATCCTCCT CTTCAACATC GTCATCATCT TCACCATCTT CCTCTCCATC CTCTGCCTCA 
GACAGACAGA CTTTTGCTGC AAGCTCAGAC TCCTCTTCTT CCTCTTCCTC CTCCACTGCT 
GCTGTTGCCA CTGATCCGCT GGATGATGGG GCTTCAGTCA TTACCACTGG CACAGCAGGC 
ACTGGAGCAA GCAGCAGAGA AAGCAGCCCT GCAGCCAGTC CCGCTACATC AAGCCCAGCA 
ATTCAGCTCA AGGAACAGAA GAGAAGACCA GACGAGTCCC AGGCCTTCAC CAGCTTTCCC 
GAGAAAAGGG CGCGGCTGGA TGAGCGTCAG TCCTTTCGTA ACACAGTTGA CTGTGTGCAC 
TCAGAAAAGC CACAGCCTAC AACAGAGGAG CCCAAGGTCC CGCCAATCCG GATACAGCTC 
TCCCGGATCA AACCGCCCTG GGTCAAGGGG CCGCCAACGT ACCAAATCTG TCCCCGCATC 
GTCCCCCCCA ACGAAGGGTC GCGGCGCTCT GGGACGGGGG CGCGCACCTT GGCGGACATC 
AAAGCCCGTG CACAGCAAGC CCGTGCCCAG CGGGAAGCCG CTGCTGCTGT TGCAGCCTCT 
GGGGATGGGG GAGGGCCTGG GGGGAGCGGC CCGGGGGGTG GTACTGGGAT ACCGGATCGC 
TCCAGCGGGA GGCGTACAAG AGAGCACCCA GGTCCCGTTG AACCTGGAGG AGGAGGAGGG 
AGACGAGGAG GAAGAGTTGA TTTGGAGGAG CAGGAATCGC CTGCGAGCTC TCATTCGTCG 
GGAGCACAAC TACAGCTGTC TAGTGTAGAT TCGGCAGACA GGCCTCAAAC TTCCACCCCA 
CTCACTGAAC CATCTCCCTC TTCTGTGTCC TCAAACCCAT CTCTACCATC ATCAGAATCT 
CCAAAAATAC TTGCGCTATC ACCACCTGCA ACCGACAGCC CTGCTTCCCA GGATCAGGTG 
GAGGAGATTT GTGGAGTGGA AGAGGTGACT GCCTGCCCAA GTGATAAAAC CTCAGAGCAG 
ACCTTAGACA CCCCAGTGCC TACCCCAAGT GAATCAGAGT CTTTTTGCAG TCACCAAGAT 
GGGAGAGGCG AGGAAGCAGA GTCGAGTGAT AGTAGAACAA ATACGCCTGT TTGCACCTCA 
TCATCAAATG ATGCTATTTC TTTAGTGCCC ACCTCTATAC CTGACTCGCT ACCAAGATTT 
GGTGCCCAGG GTGTGGATGT CATCAGGACA TTGGCAGCTT CCTCTCAGCC TTGGGAAGGT 
GAAAAGAAAG CGGTTGAGCT TCACCCAGGG ACAACTGGAG TTATCCAGCA TGGCTCAGAC 
TTGAAATTAC CCAAAGAGAC TCTTGTTACA GCCAGAAATG GATGGGTAGA AAATAGAGAA 
GAGCATTCCA TGAGAGAGGG TATGCTAGAA GAACATAGGA ATGGAGGGAG TGATGCTAAT 
GGTAAATATG AATCAGGCTC TTTACCTTGC CTACCACGAT GTGCTGGTGA GGAAGACACT 
GGAGCACACA GTGATTCAAC GGAGACAGCA TCTGACTTTG AAAATGAATC ACAAGAGGAT 
GAAATGGTGG ATTGGCATGG GACCCAGATG GATGGTAGTG GCATGCAAAC TCAAAATACA 
AAATCACTGA ATCGACCTGT TATTCAGACC TCAAGTCGAC TCACTTCATC GATTGTTAAT 
CCTCCTCAAC ACCAGCAACC GGTTATACAG GCCCATATAT CTAATCAAAA CCATACCCAA 
ACTGTTATTC AGGCTTGCTT TCCTAATGGT GTGCCAAACC AACCAGTCAT TCATCCTCAC 
AAGCAGCATG CAATTAATAC CCATGCTATA AACCACTCTC AGGACCAGAT TGCCTCTGCA 
CCTTCACAAG CCCAAGTGCA AGCTTATCTG ACCCAGACTG AGATGGACCA AAGCAGAAAT 
CTACGACTGA ATGATAATGG CAATGGTGTG AAGCTGTATT TGTCAGAGGA TGACACCAAA 
CCTCTTGGCA GGGAAGATTT AGTTTTAAAG AATTCTGCTG GTCCTCCAGT TGGCAGAAGA 
GTCCAAGTTT CATCTCGACC AGTTTCCACT GTAGAGGCCA ACAATCCTCT TGTCACACAG 
CTCCTCCAAG GCAGTCTACC TTTAGAGAAG GTCTTACCCC AACCCCACTC AGCCAGCAAG 
TTAGAAATTA ATCGACTTCC AGGTGCCAAT TCAAGCAACT CGCTCAACCC AGCCAATCAT 
CAAACACAGA GAAATCTGGC ACCACGTTTT AGAGGCCCAC CTGAGTCGGG AGGGCCTACG 
GACACTGTTA GTTCTGACTT CCAACACAAA ACCCCACCTG CTCGAGTATC TCCTGGACCT 
GGTCGGAACT TTGGTTCTTC CCCGCCAGGT TCTGCTCCAT CAAGAATGGC ATGCCTGTTT 
GAGGAAGCCA GCCCCAGGTC TGCAAACTCT CAGTTCAGTT CTCAGCAACC AGGAGGCGTT 
GTGCCTCCAG GAGCAGTACC TGTCATAACA TCCCTTCCCT TAAACTCCAC CACTAGAACC 
TCAATGGATA TGAACTCTCA GAATGCTCCA GTTTATGAAT CAGCTGTTAT TAAAGAGCAC 
CCTGGACCTC TTTCTCAACG GAGTGAAACT CCTGAGAGGC CAAGTAGCTT TCAGCAGCAT 
CCTAATAATG TCTCCCAGTC CGTGTGCAGA AACACACCAG ATGCTCCTTC ACCTCCACAT 
GGAGACCTTT GTCCATCAGA GGTTGTACCA ACTGTTAAGA TTAACTGGCG CCCAAACAAA 
CCTCAGCAAC CGATCTCCCC TGTAGCTACA GTAAAAAACG AAGTCTCCTT GCGCCCTTCG 
TGCCAACAAG CTCTGACCAA AAACTCACCT GCTCCTATCA GTGGCAATAC GTCTGTTGTA 
ATAACCAAAA AGGAGCCTCT GGATGGTTTT CATGGAGGTG GTGGAGCTAT GGAAGGACTG 
CTAAATATGG AAATGTCTCT TGTCAGAATG GCCAAGAAGG AACAAGTCAA AAATCCATAT 
ACCCGCCAAG CAGACACCTC CGCTTCTCCT GTTTCTTCCT CTCCTTCCTC ATCAGCCTCC 
AGTCTCCCTT TCCAGCTATA TGGTAAGCTA CCAAAACTTC AGCAGAGTGG GGGCAACGGA 
GGGTCCTCAT CCAGCTTCAG TTACACAGCT AATGTGTCTG TGGTGGATGG TAGTGGCTTC 
ACTCGCACCT TAGCCGATAG TGTGCTCCAA CTAAGGCCAC GTGTGAGCGT CGGCAGCGGT 
GGAGGTCAGA GTGCCACGCT TGGCATCCAG GCATTTACAG ACAGTGCTGC CGAAGAAGTG 
GCTCTCAAGT GTTCCTGCCG ACTCAAGGCT ATGATCATGT GCCAGGGCTG TGGCGCTTTC 
TGCCATGATG ATTGCATCGG CCCTTCGAAA CTGTGTGTGT CATGTCTGGT TGTCAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162395.1
CDS	482..5461
Translation	MKDKQKRKKERTWAEAARMVLENFSDAPMTPKQILHVIQTKGLKEMRSGTAPLACLVTMLHSQVRGDRVKNSIFFKLPGRMSLFTLKKNALQWTKSPSANESGDGTPTASTTSTVEGAEQESCDSTETAAASGENDASVDETSSSASCSTEPQTRLSRSSQSGRQRKKAVMMPRVVLTPLKVNGEHVPSGAAGRRREGSRGGPGPTLRTRSELGWKRTQHFKSIRGLRSGPMKRSRGGVEVDFETPGSILVNTNIRALINTRTFAAFPSHSQQQLLQLLPEVDRQVGPDGLARLSSSALNNEFFTHASQSWKERLAEGEFTHEMQVRFRQEMEKEKKVEAWKEKFFEEYHGQRSGLTREEALKLTMSEAGDVASPVLDSDSATVATPKRRSVGRRRREGRIRRRSRADLRRRARRTLCKTTTATVATEETTESTATPEEVPSVASPVPETTAVQGEVVLQTDPELEAPEENLSTEAVPSPVPVPTPPPASTSSTCDEPETSTRLLPEVPEPAVASTSSPSSSSSSTSSSSSPSSSPSSASDRQTFAASSDSSSSSSSSTAAVATDPLDDGASVITTGTAGTGASSRESSPAASPATSSPAIQLKEQKRRPDESQAFTSFPEKRARLDERQSFRNTVDCVHSEKPQPTTEEPKVPPIRIQLSRIKPPWVKGPPTYQICPRIVPPNEGSRRSGTGARTLADIKARAQQARAQREAAAAVAASGDGGGPGGSGPGGGTGIPDRSSGRRTREHPGPVEPGGGGGRRGGRVDLEEQESPASSHSSGAQLQLSSVDSADRPQTSTPLTEPSPSSVSSNPSLPSSESPKILALSPPATDSPASQDQVEEICGVEEVTACPSDKTSEQTLDTPVPTPSESESFCSHQDGRGEEAESSDSRTNTPVCTSSSNDAISLVPTSIPDSLPRFGAQGVDVIRTLAASSQPWEGEKKAVELHPGTTGVIQHGSDLKLPKETLVTARNGWVENREEHSMREGMLEEHRNGGSDANGKYESGSLPCLPRCAGEEDTGAHSDSTETASDFENESQEDEMVDWHGTQMDGSGMQTQNTKSLNRPVIQTSSRLTSSIVNPPQHQQPVIQAHISNQNHTQTVIQACFPNGVPNQPVIHPHKQHAINTHAINHSQDQIASAPSQAQVQAYLTQTEMDQSRNLRLNDNGNGVKLYLSEDDTKPLGREDLVLKNSAGPPVGRRVQVSSRPVSTVEANNPLVTQLLQGSLPLEKVLPQPHSASKLEINRLPGANSSNSLNPANHQTQRNLAPRFRGPPESGGPTDTVSSDFQHKTPPARVSPGPGRNFGSSPPGSAPSRMACLFEEASPRSANSQFSSQQPGGVVPPGAVPVITSLPLNSTTRTSMDMNSQNAPVYESAVIKEHPGPLSQRSETPERPSSFQQHPNNVSQSVCRNTPDAPSPPHGDLCPSEVVPTVKINWRPNKPQQPISPVATVKNEVSLRPSCQQALTKNSPAPISGNTSVVITKKEPLDGFHGGGGAMEGLLNMEMSLVRMAKKEQVKNPYTRQADTSASPVSSSPSSSASSLPFQLYGKLPKLQQSGGNGGSSSSFSYTANVSVVDGSGFTRTLADSVLQLRPRVSVGSGGGQSATLGIQAFTDSAAEEVALKCSCRLKAMIMCQGCGAFCHDDCIGPSKLCVSCLVVR

Target ORF information:

RefSeq Version	XM_005162338.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio additional sex combs like transcriptional regulator 1 (asxl1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162338.1

ORF Insert Sequence:

ATGAAGGACA AACAGAAGAG AAAGAAGGAG CGCACGTGGG CTGAGGCAGC TCGTATGGTT 
CTGGAGAACT TTTCTGATGC CCCAATGACG CCCAAACAGA TTCTACATGT CATCCAAACA 
AAGGGGTTGA AAGAAATGAG AAGCGGCACA GCACCACTGG CCTGCCTTGT CACCATGCTT 
CACTCTCAGG TCAGAGGTGA CCGAGTGAAA AACAGTATTT TCTTTAAGCT GCCTGGAAGG 
ATGAGTCTGT TTACCCTAAA GAAAAATGCA CTTCAGTGGA CAAAGAGCCC ATCAGCGAAT 
GAGTCTGGAG ATGGGACACC AACAGCATCC ACCACATCAA CAGTTGAAGG AGCAGAGCAG 
GAAAGCTGCG ACTCCACAGA GACGGCTGCT GCTAGTGGTG AAAATGATGC CTCTGTGGAT 
GAGACTTCCT CCAGTGCCTC TTGCTCTACA GAACCACAGA CAAGACTCAG TCGCTCTTCA 
CAGTCAGGGA GACAGAGGAA GAAGGCTGTG ATGATGCCCC GTGTAGTTCT CACTCCTCTC 
AAGGTCAACG GTGAACACGT CCCATCAGGA GCAGCGGGGA GGCGCAGGGA AGGCTCTAGG 
GGGGGTCCAG GCCCCACTCT CCGTACCCGC TCCGAGCTGG GCTGGAAACG CACCCAGCAC 
TTCAAGAGCA TTCGTGGTCT TCGTTCAGGT CCCATGAAAA GGAGTCGAGG AGGAGTGGAG 
GTGGATTTTG AGACTCCAGG ATCCATCCTG GTCAACACCA ACATTCGTGC TCTCATCAAC 
ACACGGACCT TTGCTGCTTT TCCTTCTCAC TCCCAGCAGC AACTACTCCA GCTTTTACCA 
GAGGTGGACA GACAGGTCGG CCCCGATGGC CTTGCTCGTC TAAGCAGTTC GGCCCTCAAT 
AATGAATTCT TCACTCATGC CTCCCAGAGC TGGAAAGAAC GTCTAGCTGA GGGTGAGTTT 
ACACATGAGA TGCAGGTTCG CTTCAGGCAG GAGATGGAAA AGGAGAAGAA AGTAGAAGCA 
TGGAAGGAGA AATTCTTCGA GGAGTACCAT GGCCAAAGGT CTGGCTTAAC AAGAGAGGAG 
GCATTGAAGC TCACAATGAG CGAAGCTGGA GATGTTGCTA GTCCAGTGCT AGACTCCGAT 
TCTGCTACAG TGGCTACACC CAAGAGGCGA AGTGTTGGAA GAAGACGGAG AGAAGGCCGT 
ATCAGACGAC GTTCACGGGC TGACCTCAGG CGCAGGGCAA GGCGCACACT TTGCAAAACT 
ACAACTGCAA CTGTGGCTAC AGAAGAAACA ACAGAGAGCA CTGCAACTCC AGAAGAGGTA 
CCATCAGTTG CATCACCAGT CCCAGAAACC ACAGCAGTTC AAGGAGAGGT GGTCCTCCAA 
ACAGACCCTG AACTTGAAGC ACCAGAAGAA AACCTCTCTA CAGAGGCAGT ACCTTCTCCA 
GTTCCTGTAC CCACACCTCC TCCAGCATCG ACAAGCTCAA CCTGCGATGA GCCAGAAACC 
TCTACACGCC TGTTGCCAGA AGTACCCGAA CCAGCTGTTG CATCCACCTC ATCTCCTTCC 
TCATCCTCCT CTTCAACATC GTCATCATCT TCACCATCTT CCTCTCCATC CTCTGCCTCA 
GACAGACAGA CTTTTGCTGC AAGCTCAGAC TCCTCTTCTT CCTCTTCCTC CTCCACTGCT 
GCTGTTGCCA CTGATCCGCT GGATGATGGG GCTTCAGTCA TTACCACTGG CACAGCAGGC 
ACTGGAGCAA GCAGCAGAGA AAGCAGCCCT GCAGCCAGTC CCGCTACATC AAGCCCAGCA 
ATTCAGCTCA AGGAACAGAA GAGAAGACCA GACGAGTCCC AGGCCTTCAC CAGCTTTCCC 
GAGAAAAGGG CGCGGCTGGA TGAGCGTCAG TCCTTTCGTA ACACAGTTGA CTGTGTGCAC 
TCAGAAAAGC CACAGCCTAC AACAGAGGAG CCCAAGGTCC CGCCAATCCG GATACAGCTC 
TCCCGGATCA AACCGCCCTG GGTCAAGGGG CCGCCAACGT ACCAAATCTG TCCCCGCATC 
GTCCCCCCCA ACGAAGGGTC GCGGCGCTCT GGGACGGGGG CGCGCACCTT GGCGGACATC 
AAAGCCCGTG CACAGCAAGC CCGTGCCCAG CGGGAAGCCG CTGCTGCTGT TGCAGCCTCT 
GGGGATGGGG GAGGGCCTGG GGGGAGCGGC CCGGGGGGTG GTACTGGGAT ACCGGATCGC 
TCCAGCGGGA GGCGTACAAG AGAGCACCCA GGTCCCGTTG AACCTGGAGG AGGAGGAGGG 
AGACGAGGAG GAAGAGTTGA TTTGGAGGAG CAGGAATCGC CTGCGAGCTC TCATTCGTCG 
GGAGCACAAC TACAGCTGTC TAGTGTAGAT TCGGCAGACA GGCCTCAAAC TTCCACCCCA 
CTCACTGAAC CATCTCCCTC TTCTGTGTCC TCAAACCCAT CTCTACCATC ATCAGAATCT 
CCAAAAATAC TTGCGCTATC ACCACCTGCA ACCGACAGCC CTGCTTCCCA GGATCAGGTG 
GAGGAGATTT GTGGAGTGGA AGAGGTGACT GCCTGCCCAA GTGATAAAAC CTCAGAGCAG 
ACCTTAGACA CCCCAGTGCC TACCCCAAGT GAATCAGAGT CTTTTTGCAG TCACCAAGAT 
GGGAGAGGCG AGGAAGCAGA GTCGAGTGAT AGTAGAACAA ATACGCCTGT TTGCACCTCA 
TCATCAAATG ATGCTATTTC TTTAGTGCCC ACCTCTATAC CTGACTCGCT ACCAAGATTT 
GGTGCCCAGG GTGTGGATGT CATCAGGACA TTGGCAGCTT CCTCTCAGCC TTGGGAAGGT 
GAAAAGAAAG CGGTTGAGCT TCACCCAGGG ACAACTGGAG TTATCCAGCA TGGCTCAGAC 
TTGAAATTAC CCAAAGAGAC TCTTGTTACA GCCAGAAATG GATGGGTAGA AAATAGAGAA 
GAGCATTCCA TGAGAGAGGG TATGCTAGAA GAACATAGGA ATGGAGGGAG TGATGCTAAT 
GGTAAATATG AATCAGGCTC TTTACCTTGC CTACCACGAT GTGCTGGTGA GGAAGACACT 
GGAGCACACA GTGATTCAAC GGAGACAGCA TCTGACTTTG AAAATGAATC ACAAGAGGAT 
GAAATGGTGG ATTGGCATGG GACCCAGATG GATGGTAGTG GCATGCAAAC TCAAAATACA 
AAATCACTGA ATCGACCTGT TATTCAGACC TCAAGTCGAC TCACTTCATC GATTGTTAAT 
CCTCCTCAAC ACCAGCAACC GGTTATACAG GCCCATATAT CTAATCAAAA CCATACCCAA 
ACTGTTATTC AGGCTTGCTT TCCTAATGGT GTGCCAAACC AACCAGTCAT TCATCCTCAC 
AAGCAGCATG CAATTAATAC CCATGCTATA AACCACTCTC AGGACCAGAT TGCCTCTGCA 
CCTTCACAAG CCCAAGTGCA AGCTTATCTG ACCCAGACTG AGATGGACCA AAGCAGAAAT 
CTACGACTGA ATGATAATGG CAATGGTGTG AAGCTGTATT TGTCAGAGGA TGACACCAAA 
CCTCTTGGCA GGGAAGATTT AGTTTTAAAG AATTCTGCTG GTCCTCCAGT TGGCAGAAGA 
GTCCAAGTTT CATCTCGACC AGTTTCCACT GTAGAGGCCA ACAATCCTCT TGTCACACAG 
CTCCTCCAAG GCAGTCTACC TTTAGAGAAG GTCTTACCCC AACCCCACTC AGCCAGCAAG 
TTAGAAATTA ATCGACTTCC AGGTGCCAAT TCAAGCAACT CGCTCAACCC AGCCAATCAT 
CAAACACAGA GAAATCTGGC ACCACGTTTT AGAGGCCCAC CTGAGTCGGG AGGGCCTACG 
GACACTGTTA GTTCTGACTT CCAACACAAA ACCCCACCTG CTCGAGTATC TCCTGGACCT 
GGTCGGAACT TTGGTTCTTC CCCGCCAGGT TCTGCTCCAT CAAGAATGGC ATGCCTGTTT 
GAGGAAGCCA GCCCCAGGTC TGCAAACTCT CAGTTCAGTT CTCAGCAACC AGGAGGCGTT 
GTGCCTCCAG GAGCAGTACC TGTCATAACA TCCCTTCCCT TAAACTCCAC CACTAGAACC 
TCAATGGATA TGAACTCTCA GAATGCTCCA GTTTATGAAT CAGCTGTTAT TAAAGAGCAC 
CCTGGACCTC TTTCTCAACG GAGTGAAACT CCTGAGAGGC CAAGTAGCTT TCAGCAGCAT 
CCTAATAATG TCTCCCAGTC CGTGTGCAGA AACACACCAG ATGCTCCTTC ACCTCCACAT 
GGAGACCTTT GTCCATCAGA GGTTGTACCA ACTGTTAAGA TTAACTGGCG CCCAAACAAA 
CCTCAGCAAC CGATCTCCCC TGTAGCTACA GTAAAAAACG AAGTCTCCTT GCGCCCTTCG 
TGCCAACAAG CTCTGACCAA AAACTCACCT GCTCCTATCA GTGGCAATAC GTCTGTTGTA 
ATAACCAAAA AGGAGCCTCT GGATGGTTTT CATGGAGGTG GTGGAGCTAT GGAAGGACTG 
CTAAATATGG AAATGTCTCT TGTCAGAATG GCCAAGAAGG AACAAGTCAA AAATCCATAT 
ACCCGCCAAG CAGACACCTC CGCTTCTCCT GTTTCTTCCT CTCCTTCCTC ATCAGCCTCC 
AGTCTCCCTT TCCAGCTATA TGGTAAGCTA CCAAAACTTC AGCAGAGTGG GGGCAACGGA 
GGGTCCTCAT CCAGCTTCAG TTACACAGCT AATGTGTCTG TGGTGGATGG TAGTGGCTTC 
ACTCGCACCT TAGCCGATAG TGTGCTCCAA CTAAGGCCAC GTGTGAGCGT CGGCAGCGGT 
GGAGGTCAGA GTGCCACGCT TGGCATCCAG GCATTTACAG ACAGTGCTGC CGAAGAAGTG 
GCTCTCAAGT GTTCCTGCCG ACTCAAGGCT ATGATCATGT GCCAGGGCTG TGGCGCTTTC 
TGCCATGATG ATTGCATCGG CCCTTCGAAA CTGTGTGTGT CATGTCTGGT TGTCAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

asxl1 ( XM_001341206.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001341242.2 Danio rerio additional sex combs like transcriptional regulator 1 (asxl1), transcript variant X3, mRNA.

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CloneID	ODa27916
Clone ID Related Accession (Same CDS sequence)	XM_001341206.5 , XM_021473724.1
Accession Version	XM_001341206.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	putative Polycomb group protein ASXL1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 1, 2013 this sequence version replaced XM_001341206.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGACA AACAGAAGAG AAAGAAGGAG CGCACGTGGG CTGAGGCAGC TCGTATGGTT 
CTGGAGAACT TTTCTGATGC CCCAATGACG CCCAAACAGA TTCTACATGT CATCCAAACA 
AAGGGGTTGA AAGAAATGAG AAGCGGCACA GCACCACTGG CCTGCCTTGT CACCATGCTT 
CACTCTCAGG TCAGAGGTGA CCGAGTGAAA AACAGTATTT TCTTTAAGCT GCCTGGAAGG 
ATGAGTCTGT TTACCCTAAA GAAAAATGCA CTTCAGTGGA CAAAGAGCCC ATCAGCGAAT 
GAGTCTGGAG ATGGGACACC AACAGCATCC ACCACATCAA CAGTTGAAGG AGCAGAGCAG 
GAAAGCTGCG ACTCCACAGA GACGGCTGCT GCTAGTGGTG AAAATGATGC CTCTGTGGAT 
GAGACTTCCT CCAGTGCCTC TTGCTCTACA GAACCACAGA CAAGACTCAG TCGCTCTTCA 
CAGTCAGGGA GACAGAGGAA GAAGGCTGTG ATGATGCCCC GTGTAGTTCT CACTCCTCTC 
AAGGTCAACG GTGAACACGT CCCATCAGGT CCCATGAAAA GGAGTCGAGG AGGAGTGGAG 
GTGGATTTTG AGACTCCAGG ATCCATCCTG GTCAACACCA ACATTCGTGC TCTCATCAAC 
ACACGGACCT TTGCTGCTTT TCCTTCTCAC TCCCAGCAGC AACTACTCCA GCTTTTACCA 
GAGGTGGACA GACAGGTCGG CCCCGATGGC CTTGCTCGTC TAAGCAGTTC GGCCCTCAAT 
AATGAATTCT TCACTCATGC CTCCCAGAGC TGGAAAGAAC GTCTAGCTGA GGGTGAGTTT 
ACACATGAGA TGCAGGTTCG CTTCAGGCAG GAGATGGAAA AGGAGAAGAA AGTAGAAGCA 
TGGAAGGAGA AATTCTTCGA GGAGTACCAT GGCCAAAGGT CTGGCTTAAC AAGAGAGGAG 
GCATTGAAGC TCACAATGAG CGAAGCTGGA GATGTTGCTA GTCCAGTGCT AGACTCCGAT 
TCTGCTACAG TGGCTACACC CAAGAGGCGA AGTGTTGGAA GAAGACGGAG AGAAGGCCGT 
ATCAGACGAC GTTCACGGGC TGACCTCAGG CGCAGGGCAA GGCGCACACT TTGCAAAACT 
ACAACTGCAA CTGTGGCTAC AGAAGAAACA ACAGAGAGCA CTGCAACTCC AGAAGAGGTA 
CCATCAGTTG CATCACCAGT CCCAGAAACC ACAGCAGTTC AAGGAGAGGT GGTCCTCCAA 
ACAGACCCTG AACTTGAAGC ACCAGAAGAA AACCTCTCTA CAGAGGCAGT ACCTTCTCCA 
GTTCCTGTAC CCACACCTCC TCCAGCATCG ACAAGCTCAA CCTGCGATGA GCCAGAAACC 
TCTACACGCC TGTTGCCAGA AGTACCCGAA CCAGCTGTTG CATCCACCTC ATCTCCTTCC 
TCATCCTCCT CTTCAACATC GTCATCATCT TCACCATCTT CCTCTCCATC CTCTGCCTCA 
GACAGACAGA CTTTTGCTGC AAGCTCAGAC TCCTCTTCTT CCTCTTCCTC CTCCACTGCT 
GCTGTTGCCA CTGATCCGCT GGATGATGGG GCTTCAGTCA TTACCACTGG CACAGCAGGC 
ACTGGAGCAA GCAGCAGAGA AAGCAGCCCT GCAGCCAGTC CCGCTACATC AAGCCCAGCA 
ATTCAGCTCA AGGAACAGAA GAGAAGACCA GACGAGTCCC AGGCCTTCAC CAGCTTTCCC 
GAGAAAAGGG CGCGGCTGGA TGAGCGTCAG TCCTTTCGTA ACACAGTTGA CTGTGTGCAC 
TCAGAAAAGC CACAGCCTAC AACAGAGGAG CCCAAGGTCC CGCCAATCCG GATACAGCTC 
TCCCGGATCA AACCGCCCTG GGTCAAGGGG CCGCCAACGT ACCAAATCTG TCCCCGCATC 
GTCCCCCCCA ACGAAGGGTC GCGGCGCTCT GGGACGGGGG CGCGCACCTT GGCGGACATC 
AAAGCCCGTG CACAGCAAGC CCGTGCCCAG CGGGAAGCCG CTGCTGCTGT TGCAGCCTCT 
GGGGATGGGG GAGGGCCTGG GGGGAGCGGC CCGGGGGGTG GTACTGGGAT ACCGGATCGC 
TCCAGCGGGA GGCGTACAAG AGAGCACCCA GGTCCCGTTG AACCTGGAGG AGGAGGAGGG 
AGACGAGGAG GAAGAGTTGA TTTGGAGGAG CAGGAATCGC CTGCGAGCTC TCATTCGTCG 
GGAGCACAAC TACAGCTGTC TAGTGTAGAT TCGGCAGACA GGCCTCAAAC TTCCACCCCA 
CTCACTGAAC CATCTCCCTC TTCTGTGTCC TCAAACCCAT CTCTACCATC ATCAGAATCT 
CCAAAAATAC TTGCGCTATC ACCACCTGCA ACCGACAGCC CTGCTTCCCA GGATCAGGTG 
GAGGAGATTT GTGGAGTGGA AGAGGTGACT GCCTGCCCAA GTGATAAAAC CTCAGAGCAG 
ACCTTAGACA CCCCAGTGCC TACCCCAAGT GAATCAGAGT CTTTTTGCAG TCACCAAGAT 
GGGAGAGGCG AGGAAGCAGA GTCGAGTGAT AGTAGAACAA ATACGCCTGT TTGCACCTCA 
TCATCAAATG ATGCTATTTC TTTAGTGCCC ACCTCTATAC CTGACTCGCT ACCAAGATTT 
GGTGCCCAGG GTGTGGATGT CATCAGGACA TTGGCAGCTT CCTCTCAGCC TTGGGAAGGT 
GAAAAGAAAG CGGTTGAGCT TCACCCAGGG ACAACTGGAG TTATCCAGCA TGGCTCAGAC 
TTGAAATTAC CCAAAGAGAC TCTTGTTACA GCCAGAAATG GATGGGTAGA AAATAGAGAA 
GAGCATTCCA TGAGAGAGGG TATGCTAGAA GAACATAGGA ATGGAGGGAG TGATGCTAAT 
GGTAAATATG AATCAGGCTC TTTACCTTGC CTACCACGAT GTGCTGGTGA GGAAGACACT 
GGAGCACACA GTGATTCAAC GGAGACAGCA TCTGACTTTG AAAATGAATC ACAAGAGGAT 
GAAATGGTGG ATTGGCATGG GACCCAGATG GATGGTAGTG GCATGCAAAC TCAAAATACA 
AAATCACTGA ATCGACCTGT TATTCAGACC TCAAGTCGAC TCACTTCATC GATTGTTAAT 
CCTCCTCAAC ACCAGCAACC GGTTATACAG GCCCATATAT CTAATCAAAA CCATACCCAA 
ACTGTTATTC AGGCTTGCTT TCCTAATGGT GTGCCAAACC AACCAGTCAT TCATCCTCAC 
AAGCAGCATG CAATTAATAC CCATGCTATA AACCACTCTC AGGACCAGAT TGCCTCTGCA 
CCTTCACAAG CCCAAGTGCA AGCTTATCTG ACCCAGACTG AGATGGACCA AAGCAGAAAT 
CTACGACTGA ATGATAATGG CAATGGTGTG AAGCTGTATT TGTCAGAGGA TGACACCAAA 
CCTCTTGGCA GGGAAGATTT AGTTTTAAAG AATTCTGCTG GTCCTCCAGT TGGCAGAAGA 
GTCCAAGTTT CATCTCGACC AGTTTCCACT GTAGAGGCCA ACAATCCTCT TGTCACACAG 
CTCCTCCAAG GCAGTCTACC TTTAGAGAAG GTCTTACCCC AACCCCACTC AGCCAGCAAG 
TTAGAAATTA ATCGACTTCC AGGTGCCAAT TCAAGCAACT CGCTCAACCC AGCCAATCAT 
CAAACACAGA GAAATCTGGC ACCACGTTTT AGAGGCCCAC CTGAGTCGGG AGGGCCTACG 
GACACTGTTA GTTCTGACTT CCAACACAAA ACCCCACCTG CTCGAGTATC TCCTGGACCT 
GGTCGGAACT TTGGTTCTTC CCCGCCAGGT TCTGCTCCAT CAAGAATGGC ATGCCTGTTT 
GAGGAAGCCA GCCCCAGGTC TGCAAACTCT CAGTTCAGTT CTCAGCAACC AGGAGGCGTT 
GTGCCTCCAG GAGCAGTACC TGTCATAACA TCCCTTCCCT TAAACTCCAC CACTAGAACC 
TCAATGGATA TGAACTCTCA GAATGCTCCA GTTTATGAAT CAGCTGTTAT TAAAGAGCAC 
CCTGGACCTC TTTCTCAACG GAGTGAAACT CCTGAGAGGC CAAGTAGCTT TCAGCAGCAT 
CCTAATAATG TCTCCCAGTC CGTGTGCAGA AACACACCAG ATGCTCCTTC ACCTCCACAT 
GGAGACCTTT GTCCATCAGA GGTTGTACCA ACTGTTAAGA TTAACTGGCG CCCAAACAAA 
CCTCAGCAAC CGATCTCCCC TGTAGCTACA GTAAAAAACG AAGTCTCCTT GCGCCCTTCG 
TGCCAACAAG CTCTGACCAA AAACTCACCT GCTCCTATCA GTGGCAATAC GTCTGTTGTA 
ATAACCAAAA AGGAGCCTCT GGATGGTTTT CATGGAGGTG GTGGAGCTAT GGAAGGACTG 
CTAAATATGG AAATGTCTCT TGTCAGAATG GCCAAGAAGG AACAAGTCAA AAATCCATAT 
ACCCGCCAAG CAGACACCTC CGCTTCTCCT GTTTCTTCCT CTCCTTCCTC ATCAGCCTCC 
AGTCTCCCTT TCCAGCTATA TGGTAAGCTA CCAAAACTTC AGCAGAGTGG GGGCAACGGA 
GGGTCCTCAT CCAGCTTCAG TTACACAGCT AATGTGTCTG TGGTGGATGG TAGTGGCTTC 
ACTCGCACCT TAGCCGATAG TGTGCTCCAA CTAAGGCCAC GTGTGAGCGT CGGCAGCGGT 
GGAGGTCAGA GTGCCACGCT TGGCATCCAG GCATTTACAG ACAGTGCTGC CGAAGAAGTG 
GCTCTCAAGT GTTCCTGCCG ACTCAAGGCT ATGATCATGT GCCAGGGCTG TGGCGCTTTC 
TGCCATGATG ATTGCATCGG CCCTTCGAAA CTGTGTGTGT CATGTCTGGT TGTCAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001341242.2
CDS	482..5341
Translation	MKDKQKRKKERTWAEAARMVLENFSDAPMTPKQILHVIQTKGLKEMRSGTAPLACLVTMLHSQVRGDRVKNSIFFKLPGRMSLFTLKKNALQWTKSPSANESGDGTPTASTTSTVEGAEQESCDSTETAAASGENDASVDETSSSASCSTEPQTRLSRSSQSGRQRKKAVMMPRVVLTPLKVNGEHVPSGPMKRSRGGVEVDFETPGSILVNTNIRALINTRTFAAFPSHSQQQLLQLLPEVDRQVGPDGLARLSSSALNNEFFTHASQSWKERLAEGEFTHEMQVRFRQEMEKEKKVEAWKEKFFEEYHGQRSGLTREEALKLTMSEAGDVASPVLDSDSATVATPKRRSVGRRRREGRIRRRSRADLRRRARRTLCKTTTATVATEETTESTATPEEVPSVASPVPETTAVQGEVVLQTDPELEAPEENLSTEAVPSPVPVPTPPPASTSSTCDEPETSTRLLPEVPEPAVASTSSPSSSSSSTSSSSSPSSSPSSASDRQTFAASSDSSSSSSSSTAAVATDPLDDGASVITTGTAGTGASSRESSPAASPATSSPAIQLKEQKRRPDESQAFTSFPEKRARLDERQSFRNTVDCVHSEKPQPTTEEPKVPPIRIQLSRIKPPWVKGPPTYQICPRIVPPNEGSRRSGTGARTLADIKARAQQARAQREAAAAVAASGDGGGPGGSGPGGGTGIPDRSSGRRTREHPGPVEPGGGGGRRGGRVDLEEQESPASSHSSGAQLQLSSVDSADRPQTSTPLTEPSPSSVSSNPSLPSSESPKILALSPPATDSPASQDQVEEICGVEEVTACPSDKTSEQTLDTPVPTPSESESFCSHQDGRGEEAESSDSRTNTPVCTSSSNDAISLVPTSIPDSLPRFGAQGVDVIRTLAASSQPWEGEKKAVELHPGTTGVIQHGSDLKLPKETLVTARNGWVENREEHSMREGMLEEHRNGGSDANGKYESGSLPCLPRCAGEEDTGAHSDSTETASDFENESQEDEMVDWHGTQMDGSGMQTQNTKSLNRPVIQTSSRLTSSIVNPPQHQQPVIQAHISNQNHTQTVIQACFPNGVPNQPVIHPHKQHAINTHAINHSQDQIASAPSQAQVQAYLTQTEMDQSRNLRLNDNGNGVKLYLSEDDTKPLGREDLVLKNSAGPPVGRRVQVSSRPVSTVEANNPLVTQLLQGSLPLEKVLPQPHSASKLEINRLPGANSSNSLNPANHQTQRNLAPRFRGPPESGGPTDTVSSDFQHKTPPARVSPGPGRNFGSSPPGSAPSRMACLFEEASPRSANSQFSSQQPGGVVPPGAVPVITSLPLNSTTRTSMDMNSQNAPVYESAVIKEHPGPLSQRSETPERPSSFQQHPNNVSQSVCRNTPDAPSPPHGDLCPSEVVPTVKINWRPNKPQQPISPVATVKNEVSLRPSCQQALTKNSPAPISGNTSVVITKKEPLDGFHGGGGAMEGLLNMEMSLVRMAKKEQVKNPYTRQADTSASPVSSSPSSSASSLPFQLYGKLPKLQQSGGNGGSSSSFSYTANVSVVDGSGFTRTLADSVLQLRPRVSVGSGGGQSATLGIQAFTDSAAEEVALKCSCRLKAMIMCQGCGAFCHDDCIGPSKLCVSCLVVR

Target ORF information:

RefSeq Version	XM_001341206.5
Organism	Danio rerio(zebrafish)
Definition	Danio rerio additional sex combs like transcriptional regulator 1 (asxl1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001341206.5

ORF Insert Sequence:

ATGAAGGACA AACAGAAGAG AAAGAAGGAG CGCACGTGGG CTGAGGCAGC TCGTATGGTT 
CTGGAGAACT TTTCTGATGC CCCAATGACG CCCAAACAGA TTCTACATGT CATCCAAACA 
AAGGGGTTGA AAGAAATGAG AAGCGGCACA GCACCACTGG CCTGCCTTGT CACCATGCTT 
CACTCTCAGG TCAGAGGTGA CCGAGTGAAA AACAGTATTT TCTTTAAGCT GCCTGGAAGG 
ATGAGTCTGT TTACCCTAAA GAAAAATGCA CTTCAGTGGA CAAAGAGCCC ATCAGCGAAT 
GAGTCTGGAG ATGGGACACC AACAGCATCC ACCACATCAA CAGTTGAAGG AGCAGAGCAG 
GAAAGCTGCG ACTCCACAGA GACGGCTGCT GCTAGTGGTG AAAATGATGC CTCTGTGGAT 
GAGACTTCCT CCAGTGCCTC TTGCTCTACA GAACCACAGA CAAGACTCAG TCGCTCTTCA 
CAGTCAGGGA GACAGAGGAA GAAGGCTGTG ATGATGCCCC GTGTAGTTCT CACTCCTCTC 
AAGGTCAACG GTGAACACGT CCCATCAGGT CCCATGAAAA GGAGTCGAGG AGGAGTGGAG 
GTGGATTTTG AGACTCCAGG ATCCATCCTG GTCAACACCA ACATTCGTGC TCTCATCAAC 
ACACGGACCT TTGCTGCTTT TCCTTCTCAC TCCCAGCAGC AACTACTCCA GCTTTTACCA 
GAGGTGGACA GACAGGTCGG CCCCGATGGC CTTGCTCGTC TAAGCAGTTC GGCCCTCAAT 
AATGAATTCT TCACTCATGC CTCCCAGAGC TGGAAAGAAC GTCTAGCTGA GGGTGAGTTT 
ACACATGAGA TGCAGGTTCG CTTCAGGCAG GAGATGGAAA AGGAGAAGAA AGTAGAAGCA 
TGGAAGGAGA AATTCTTCGA GGAGTACCAT GGCCAAAGGT CTGGCTTAAC AAGAGAGGAG 
GCATTGAAGC TCACAATGAG CGAAGCTGGA GATGTTGCTA GTCCAGTGCT AGACTCCGAT 
TCTGCTACAG TGGCTACACC CAAGAGGCGA AGTGTTGGAA GAAGACGGAG AGAAGGCCGT 
ATCAGACGAC GTTCACGGGC TGACCTCAGG CGCAGGGCAA GGCGCACACT TTGCAAAACT 
ACAACTGCAA CTGTGGCTAC AGAAGAAACA ACAGAGAGCA CTGCAACTCC AGAAGAGGTA 
CCATCAGTTG CATCACCAGT CCCAGAAACC ACAGCAGTTC AAGGAGAGGT GGTCCTCCAA 
ACAGACCCTG AACTTGAAGC ACCAGAAGAA AACCTCTCTA CAGAGGCAGT ACCTTCTCCA 
GTTCCTGTAC CCACACCTCC TCCAGCATCG ACAAGCTCAA CCTGCGATGA GCCAGAAACC 
TCTACACGCC TGTTGCCAGA AGTACCCGAA CCAGCTGTTG CATCCACCTC ATCTCCTTCC 
TCATCCTCCT CTTCAACATC GTCATCATCT TCACCATCTT CCTCTCCATC CTCTGCCTCA 
GACAGACAGA CTTTTGCTGC AAGCTCAGAC TCCTCTTCTT CCTCTTCCTC CTCCACTGCT 
GCTGTTGCCA CTGATCCGCT GGATGATGGG GCTTCAGTCA TTACCACTGG CACAGCAGGC 
ACTGGAGCAA GCAGCAGAGA AAGCAGCCCT GCAGCCAGTC CCGCTACATC AAGCCCAGCA 
ATTCAGCTCA AGGAACAGAA GAGAAGACCA GACGAGTCCC AGGCCTTCAC CAGCTTTCCC 
GAGAAAAGGG CGCGGCTGGA TGAGCGTCAG TCCTTTCGTA ACACAGTTGA CTGTGTGCAC 
TCAGAAAAGC CACAGCCTAC AACAGAGGAG CCCAAGGTCC CGCCAATCCG GATACAGCTC 
TCCCGGATCA AACCGCCCTG GGTCAAGGGG CCGCCAACGT ACCAAATCTG TCCCCGCATC 
GTCCCCCCCA ACGAAGGGTC GCGGCGCTCT GGGACGGGGG CGCGCACCTT GGCGGACATC 
AAAGCCCGTG CACAGCAAGC CCGTGCCCAG CGGGAAGCCG CTGCTGCTGT TGCAGCCTCT 
GGGGATGGGG GAGGGCCTGG GGGGAGCGGC CCGGGGGGTG GTACTGGGAT ACCGGATCGC 
TCCAGCGGGA GGCGTACAAG AGAGCACCCA GGTCCCGTTG AACCTGGAGG AGGAGGAGGG 
AGACGAGGAG GAAGAGTTGA TTTGGAGGAG CAGGAATCGC CTGCGAGCTC TCATTCGTCG 
GGAGCACAAC TACAGCTGTC TAGTGTAGAT TCGGCAGACA GGCCTCAAAC TTCCACCCCA 
CTCACTGAAC CATCTCCCTC TTCTGTGTCC TCAAACCCAT CTCTACCATC ATCAGAATCT 
CCAAAAATAC TTGCGCTATC ACCACCTGCA ACCGACAGCC CTGCTTCCCA GGATCAGGTG 
GAGGAGATTT GTGGAGTGGA AGAGGTGACT GCCTGCCCAA GTGATAAAAC CTCAGAGCAG 
ACCTTAGACA CCCCAGTGCC TACCCCAAGT GAATCAGAGT CTTTTTGCAG TCACCAAGAT 
GGGAGAGGCG AGGAAGCAGA GTCGAGTGAT AGTAGAACAA ATACGCCTGT TTGCACCTCA 
TCATCAAATG ATGCTATTTC TTTAGTGCCC ACCTCTATAC CTGACTCGCT ACCAAGATTT 
GGTGCCCAGG GTGTGGATGT CATCAGGACA TTGGCAGCTT CCTCTCAGCC TTGGGAAGGT 
GAAAAGAAAG CGGTTGAGCT TCACCCAGGG ACAACTGGAG TTATCCAGCA TGGCTCAGAC 
TTGAAATTAC CCAAAGAGAC TCTTGTTACA GCCAGAAATG GATGGGTAGA AAATAGAGAA 
GAGCATTCCA TGAGAGAGGG TATGCTAGAA GAACATAGGA ATGGAGGGAG TGATGCTAAT 
GGTAAATATG AATCAGGCTC TTTACCTTGC CTACCACGAT GTGCTGGTGA GGAAGACACT 
GGAGCACACA GTGATTCAAC GGAGACAGCA TCTGACTTTG AAAATGAATC ACAAGAGGAT 
GAAATGGTGG ATTGGCATGG GACCCAGATG GATGGTAGTG GCATGCAAAC TCAAAATACA 
AAATCACTGA ATCGACCTGT TATTCAGACC TCAAGTCGAC TCACTTCATC GATTGTTAAT 
CCTCCTCAAC ACCAGCAACC GGTTATACAG GCCCATATAT CTAATCAAAA CCATACCCAA 
ACTGTTATTC AGGCTTGCTT TCCTAATGGT GTGCCAAACC AACCAGTCAT TCATCCTCAC 
AAGCAGCATG CAATTAATAC CCATGCTATA AACCACTCTC AGGACCAGAT TGCCTCTGCA 
CCTTCACAAG CCCAAGTGCA AGCTTATCTG ACCCAGACTG AGATGGACCA AAGCAGAAAT 
CTACGACTGA ATGATAATGG CAATGGTGTG AAGCTGTATT TGTCAGAGGA TGACACCAAA 
CCTCTTGGCA GGGAAGATTT AGTTTTAAAG AATTCTGCTG GTCCTCCAGT TGGCAGAAGA 
GTCCAAGTTT CATCTCGACC AGTTTCCACT GTAGAGGCCA ACAATCCTCT TGTCACACAG 
CTCCTCCAAG GCAGTCTACC TTTAGAGAAG GTCTTACCCC AACCCCACTC AGCCAGCAAG 
TTAGAAATTA ATCGACTTCC AGGTGCCAAT TCAAGCAACT CGCTCAACCC AGCCAATCAT 
CAAACACAGA GAAATCTGGC ACCACGTTTT AGAGGCCCAC CTGAGTCGGG AGGGCCTACG 
GACACTGTTA GTTCTGACTT CCAACACAAA ACCCCACCTG CTCGAGTATC TCCTGGACCT 
GGTCGGAACT TTGGTTCTTC CCCGCCAGGT TCTGCTCCAT CAAGAATGGC ATGCCTGTTT 
GAGGAAGCCA GCCCCAGGTC TGCAAACTCT CAGTTCAGTT CTCAGCAACC AGGAGGCGTT 
GTGCCTCCAG GAGCAGTACC TGTCATAACA TCCCTTCCCT TAAACTCCAC CACTAGAACC 
TCAATGGATA TGAACTCTCA GAATGCTCCA GTTTATGAAT CAGCTGTTAT TAAAGAGCAC 
CCTGGACCTC TTTCTCAACG GAGTGAAACT CCTGAGAGGC CAAGTAGCTT TCAGCAGCAT 
CCTAATAATG TCTCCCAGTC CGTGTGCAGA AACACACCAG ATGCTCCTTC ACCTCCACAT 
GGAGACCTTT GTCCATCAGA GGTTGTACCA ACTGTTAAGA TTAACTGGCG CCCAAACAAA 
CCTCAGCAAC CGATCTCCCC TGTAGCTACA GTAAAAAACG AAGTCTCCTT GCGCCCTTCG 
TGCCAACAAG CTCTGACCAA AAACTCACCT GCTCCTATCA GTGGCAATAC GTCTGTTGTA 
ATAACCAAAA AGGAGCCTCT GGATGGTTTT CATGGAGGTG GTGGAGCTAT GGAAGGACTG 
CTAAATATGG AAATGTCTCT TGTCAGAATG GCCAAGAAGG AACAAGTCAA AAATCCATAT 
ACCCGCCAAG CAGACACCTC CGCTTCTCCT GTTTCTTCCT CTCCTTCCTC ATCAGCCTCC 
AGTCTCCCTT TCCAGCTATA TGGTAAGCTA CCAAAACTTC AGCAGAGTGG GGGCAACGGA 
GGGTCCTCAT CCAGCTTCAG TTACACAGCT AATGTGTCTG TGGTGGATGG TAGTGGCTTC 
ACTCGCACCT TAGCCGATAG TGTGCTCCAA CTAAGGCCAC GTGTGAGCGT CGGCAGCGGT 
GGAGGTCAGA GTGCCACGCT TGGCATCCAG GCATTTACAG ACAGTGCTGC CGAAGAAGTG 
GCTCTCAAGT GTTCCTGCCG ACTCAAGGCT ATGATCATGT GCCAGGGCTG TGGCGCTTTC 
TGCCATGATG ATTGCATCGG CCCTTCGAAA CTGTGTGTGT CATGTCTGGT TGTCAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

asxl1 ( XM_021473724.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329399.1 Danio rerio additional sex combs like transcriptional regulator 1 (asxl1), transcript variant X3, mRNA.

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CloneID	ODa27916
Clone ID Related Accession (Same CDS sequence)	XM_001341206.5 , XM_021473724.1
Accession Version	XM_021473724.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	putative Polycomb group protein ASXL1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395158.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGACA AACAGAAGAG AAAGAAGGAG CGCACGTGGG CTGAGGCAGC TCGTATGGTT 
CTGGAGAACT TTTCTGATGC CCCAATGACG CCCAAACAGA TTCTACATGT CATCCAAACA 
AAGGGGTTGA AAGAAATGAG AAGCGGCACA GCACCACTGG CCTGCCTTGT CACCATGCTT 
CACTCTCAGG TCAGAGGTGA CCGAGTGAAA AACAGTATTT TCTTTAAGCT GCCTGGAAGG 
ATGAGTCTGT TTACCCTAAA GAAAAATGCA CTTCAGTGGA CAAAGAGCCC ATCAGCGAAT 
GAGTCTGGAG ATGGGACACC AACAGCATCC ACCACATCAA CAGTTGAAGG AGCAGAGCAG 
GAAAGCTGCG ACTCCACAGA GACGGCTGCT GCTAGTGGTG AAAATGATGC CTCTGTGGAT 
GAGACTTCCT CCAGTGCCTC TTGCTCTACA GAACCACAGA CAAGACTCAG TCGCTCTTCA 
CAGTCAGGGA GACAGAGGAA GAAGGCTGTG ATGATGCCCC GTGTAGTTCT CACTCCTCTC 
AAGGTCAACG GTGAACACGT CCCATCAGGT CCCATGAAAA GGAGTCGAGG AGGAGTGGAG 
GTGGATTTTG AGACTCCAGG ATCCATCCTG GTCAACACCA ACATTCGTGC TCTCATCAAC 
ACACGGACCT TTGCTGCTTT TCCTTCTCAC TCCCAGCAGC AACTACTCCA GCTTTTACCA 
GAGGTGGACA GACAGGTCGG CCCCGATGGC CTTGCTCGTC TAAGCAGTTC GGCCCTCAAT 
AATGAATTCT TCACTCATGC CTCCCAGAGC TGGAAAGAAC GTCTAGCTGA GGGTGAGTTT 
ACACATGAGA TGCAGGTTCG CTTCAGGCAG GAGATGGAAA AGGAGAAGAA AGTAGAAGCA 
TGGAAGGAGA AATTCTTCGA GGAGTACCAT GGCCAAAGGT CTGGCTTAAC AAGAGAGGAG 
GCATTGAAGC TCACAATGAG CGAAGCTGGA GATGTTGCTA GTCCAGTGCT AGACTCCGAT 
TCTGCTACAG TGGCTACACC CAAGAGGCGA AGTGTTGGAA GAAGACGGAG AGAAGGCCGT 
ATCAGACGAC GTTCACGGGC TGACCTCAGG CGCAGGGCAA GGCGCACACT TTGCAAAACT 
ACAACTGCAA CTGTGGCTAC AGAAGAAACA ACAGAGAGCA CTGCAACTCC AGAAGAGGTA 
CCATCAGTTG CATCACCAGT CCCAGAAACC ACAGCAGTTC AAGGAGAGGT GGTCCTCCAA 
ACAGACCCTG AACTTGAAGC ACCAGAAGAA AACCTCTCTA CAGAGGCAGT ACCTTCTCCA 
GTTCCTGTAC CCACACCTCC TCCAGCATCG ACAAGCTCAA CCTGCGATGA GCCAGAAACC 
TCTACACGCC TGTTGCCAGA AGTACCCGAA CCAGCTGTTG CATCCACCTC ATCTCCTTCC 
TCATCCTCCT CTTCAACATC GTCATCATCT TCACCATCTT CCTCTCCATC CTCTGCCTCA 
GACAGACAGA CTTTTGCTGC AAGCTCAGAC TCCTCTTCTT CCTCTTCCTC CTCCACTGCT 
GCTGTTGCCA CTGATCCGCT GGATGATGGG GCTTCAGTCA TTACCACTGG CACAGCAGGC 
ACTGGAGCAA GCAGCAGAGA AAGCAGCCCT GCAGCCAGTC CCGCTACATC AAGCCCAGCA 
ATTCAGCTCA AGGAACAGAA GAGAAGACCA GACGAGTCCC AGGCCTTCAC CAGCTTTCCC 
GAGAAAAGGG CGCGGCTGGA TGAGCGTCAG TCCTTTCGTA ACACAGTTGA CTGTGTGCAC 
TCAGAAAAGC CACAGCCTAC AACAGAGGAG CCCAAGGTCC CGCCAATCCG GATACAGCTC 
TCCCGGATCA AACCGCCCTG GGTCAAGGGG CCGCCAACGT ACCAAATCTG TCCCCGCATC 
GTCCCCCCCA ACGAAGGGTC GCGGCGCTCT GGGACGGGGG CGCGCACCTT GGCGGACATC 
AAAGCCCGTG CACAGCAAGC CCGTGCCCAG CGGGAAGCCG CTGCTGCTGT TGCAGCCTCT 
GGGGATGGGG GAGGGCCTGG GGGGAGCGGC CCGGGGGGTG GTACTGGGAT ACCGGATCGC 
TCCAGCGGGA GGCGTACAAG AGAGCACCCA GGTCCCGTTG AACCTGGAGG AGGAGGAGGG 
AGACGAGGAG GAAGAGTTGA TTTGGAGGAG CAGGAATCGC CTGCGAGCTC TCATTCGTCG 
GGAGCACAAC TACAGCTGTC TAGTGTAGAT TCGGCAGACA GGCCTCAAAC TTCCACCCCA 
CTCACTGAAC CATCTCCCTC TTCTGTGTCC TCAAACCCAT CTCTACCATC ATCAGAATCT 
CCAAAAATAC TTGCGCTATC ACCACCTGCA ACCGACAGCC CTGCTTCCCA GGATCAGGTG 
GAGGAGATTT GTGGAGTGGA AGAGGTGACT GCCTGCCCAA GTGATAAAAC CTCAGAGCAG 
ACCTTAGACA CCCCAGTGCC TACCCCAAGT GAATCAGAGT CTTTTTGCAG TCACCAAGAT 
GGGAGAGGCG AGGAAGCAGA GTCGAGTGAT AGTAGAACAA ATACGCCTGT TTGCACCTCA 
TCATCAAATG ATGCTATTTC TTTAGTGCCC ACCTCTATAC CTGACTCGCT ACCAAGATTT 
GGTGCCCAGG GTGTGGATGT CATCAGGACA TTGGCAGCTT CCTCTCAGCC TTGGGAAGGT 
GAAAAGAAAG CGGTTGAGCT TCACCCAGGG ACAACTGGAG TTATCCAGCA TGGCTCAGAC 
TTGAAATTAC CCAAAGAGAC TCTTGTTACA GCCAGAAATG GATGGGTAGA AAATAGAGAA 
GAGCATTCCA TGAGAGAGGG TATGCTAGAA GAACATAGGA ATGGAGGGAG TGATGCTAAT 
GGTAAATATG AATCAGGCTC TTTACCTTGC CTACCACGAT GTGCTGGTGA GGAAGACACT 
GGAGCACACA GTGATTCAAC GGAGACAGCA TCTGACTTTG AAAATGAATC ACAAGAGGAT 
GAAATGGTGG ATTGGCATGG GACCCAGATG GATGGTAGTG GCATGCAAAC TCAAAATACA 
AAATCACTGA ATCGACCTGT TATTCAGACC TCAAGTCGAC TCACTTCATC GATTGTTAAT 
CCTCCTCAAC ACCAGCAACC GGTTATACAG GCCCATATAT CTAATCAAAA CCATACCCAA 
ACTGTTATTC AGGCTTGCTT TCCTAATGGT GTGCCAAACC AACCAGTCAT TCATCCTCAC 
AAGCAGCATG CAATTAATAC CCATGCTATA AACCACTCTC AGGACCAGAT TGCCTCTGCA 
CCTTCACAAG CCCAAGTGCA AGCTTATCTG ACCCAGACTG AGATGGACCA AAGCAGAAAT 
CTACGACTGA ATGATAATGG CAATGGTGTG AAGCTGTATT TGTCAGAGGA TGACACCAAA 
CCTCTTGGCA GGGAAGATTT AGTTTTAAAG AATTCTGCTG GTCCTCCAGT TGGCAGAAGA 
GTCCAAGTTT CATCTCGACC AGTTTCCACT GTAGAGGCCA ACAATCCTCT TGTCACACAG 
CTCCTCCAAG GCAGTCTACC TTTAGAGAAG GTCTTACCCC AACCCCACTC AGCCAGCAAG 
TTAGAAATTA ATCGACTTCC AGGTGCCAAT TCAAGCAACT CGCTCAACCC AGCCAATCAT 
CAAACACAGA GAAATCTGGC ACCACGTTTT AGAGGCCCAC CTGAGTCGGG AGGGCCTACG 
GACACTGTTA GTTCTGACTT CCAACACAAA ACCCCACCTG CTCGAGTATC TCCTGGACCT 
GGTCGGAACT TTGGTTCTTC CCCGCCAGGT TCTGCTCCAT CAAGAATGGC ATGCCTGTTT 
GAGGAAGCCA GCCCCAGGTC TGCAAACTCT CAGTTCAGTT CTCAGCAACC AGGAGGCGTT 
GTGCCTCCAG GAGCAGTACC TGTCATAACA TCCCTTCCCT TAAACTCCAC CACTAGAACC 
TCAATGGATA TGAACTCTCA GAATGCTCCA GTTTATGAAT CAGCTGTTAT TAAAGAGCAC 
CCTGGACCTC TTTCTCAACG GAGTGAAACT CCTGAGAGGC CAAGTAGCTT TCAGCAGCAT 
CCTAATAATG TCTCCCAGTC CGTGTGCAGA AACACACCAG ATGCTCCTTC ACCTCCACAT 
GGAGACCTTT GTCCATCAGA GGTTGTACCA ACTGTTAAGA TTAACTGGCG CCCAAACAAA 
CCTCAGCAAC CGATCTCCCC TGTAGCTACA GTAAAAAACG AAGTCTCCTT GCGCCCTTCG 
TGCCAACAAG CTCTGACCAA AAACTCACCT GCTCCTATCA GTGGCAATAC GTCTGTTGTA 
ATAACCAAAA AGGAGCCTCT GGATGGTTTT CATGGAGGTG GTGGAGCTAT GGAAGGACTG 
CTAAATATGG AAATGTCTCT TGTCAGAATG GCCAAGAAGG AACAAGTCAA AAATCCATAT 
ACCCGCCAAG CAGACACCTC CGCTTCTCCT GTTTCTTCCT CTCCTTCCTC ATCAGCCTCC 
AGTCTCCCTT TCCAGCTATA TGGTAAGCTA CCAAAACTTC AGCAGAGTGG GGGCAACGGA 
GGGTCCTCAT CCAGCTTCAG TTACACAGCT AATGTGTCTG TGGTGGATGG TAGTGGCTTC 
ACTCGCACCT TAGCCGATAG TGTGCTCCAA CTAAGGCCAC GTGTGAGCGT CGGCAGCGGT 
GGAGGTCAGA GTGCCACGCT TGGCATCCAG GCATTTACAG ACAGTGCTGC CGAAGAAGTG 
GCTCTCAAGT GTTCCTGCCG ACTCAAGGCT ATGATCATGT GCCAGGGCTG TGGCGCTTTC 
TGCCATGATG ATTGCATCGG CCCTTCGAAA CTGTGTGTGT CATGTCTGGT TGTCAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329399.1
CDS	482..5341
Translation	MKDKQKRKKERTWAEAARMVLENFSDAPMTPKQILHVIQTKGLKEMRSGTAPLACLVTMLHSQVRGDRVKNSIFFKLPGRMSLFTLKKNALQWTKSPSANESGDGTPTASTTSTVEGAEQESCDSTETAAASGENDASVDETSSSASCSTEPQTRLSRSSQSGRQRKKAVMMPRVVLTPLKVNGEHVPSGPMKRSRGGVEVDFETPGSILVNTNIRALINTRTFAAFPSHSQQQLLQLLPEVDRQVGPDGLARLSSSALNNEFFTHASQSWKERLAEGEFTHEMQVRFRQEMEKEKKVEAWKEKFFEEYHGQRSGLTREEALKLTMSEAGDVASPVLDSDSATVATPKRRSVGRRRREGRIRRRSRADLRRRARRTLCKTTTATVATEETTESTATPEEVPSVASPVPETTAVQGEVVLQTDPELEAPEENLSTEAVPSPVPVPTPPPASTSSTCDEPETSTRLLPEVPEPAVASTSSPSSSSSSTSSSSSPSSSPSSASDRQTFAASSDSSSSSSSSTAAVATDPLDDGASVITTGTAGTGASSRESSPAASPATSSPAIQLKEQKRRPDESQAFTSFPEKRARLDERQSFRNTVDCVHSEKPQPTTEEPKVPPIRIQLSRIKPPWVKGPPTYQICPRIVPPNEGSRRSGTGARTLADIKARAQQARAQREAAAAVAASGDGGGPGGSGPGGGTGIPDRSSGRRTREHPGPVEPGGGGGRRGGRVDLEEQESPASSHSSGAQLQLSSVDSADRPQTSTPLTEPSPSSVSSNPSLPSSESPKILALSPPATDSPASQDQVEEICGVEEVTACPSDKTSEQTLDTPVPTPSESESFCSHQDGRGEEAESSDSRTNTPVCTSSSNDAISLVPTSIPDSLPRFGAQGVDVIRTLAASSQPWEGEKKAVELHPGTTGVIQHGSDLKLPKETLVTARNGWVENREEHSMREGMLEEHRNGGSDANGKYESGSLPCLPRCAGEEDTGAHSDSTETASDFENESQEDEMVDWHGTQMDGSGMQTQNTKSLNRPVIQTSSRLTSSIVNPPQHQQPVIQAHISNQNHTQTVIQACFPNGVPNQPVIHPHKQHAINTHAINHSQDQIASAPSQAQVQAYLTQTEMDQSRNLRLNDNGNGVKLYLSEDDTKPLGREDLVLKNSAGPPVGRRVQVSSRPVSTVEANNPLVTQLLQGSLPLEKVLPQPHSASKLEINRLPGANSSNSLNPANHQTQRNLAPRFRGPPESGGPTDTVSSDFQHKTPPARVSPGPGRNFGSSPPGSAPSRMACLFEEASPRSANSQFSSQQPGGVVPPGAVPVITSLPLNSTTRTSMDMNSQNAPVYESAVIKEHPGPLSQRSETPERPSSFQQHPNNVSQSVCRNTPDAPSPPHGDLCPSEVVPTVKINWRPNKPQQPISPVATVKNEVSLRPSCQQALTKNSPAPISGNTSVVITKKEPLDGFHGGGGAMEGLLNMEMSLVRMAKKEQVKNPYTRQADTSASPVSSSPSSSASSLPFQLYGKLPKLQQSGGNGGSSSSFSYTANVSVVDGSGFTRTLADSVLQLRPRVSVGSGGGQSATLGIQAFTDSAAEEVALKCSCRLKAMIMCQGCGAFCHDDCIGPSKLCVSCLVVR

Target ORF information:

RefSeq Version	XM_021473724.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio additional sex combs like transcriptional regulator 1 (asxl1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473724.1

ORF Insert Sequence:

ATGAAGGACA AACAGAAGAG AAAGAAGGAG CGCACGTGGG CTGAGGCAGC TCGTATGGTT 
CTGGAGAACT TTTCTGATGC CCCAATGACG CCCAAACAGA TTCTACATGT CATCCAAACA 
AAGGGGTTGA AAGAAATGAG AAGCGGCACA GCACCACTGG CCTGCCTTGT CACCATGCTT 
CACTCTCAGG TCAGAGGTGA CCGAGTGAAA AACAGTATTT TCTTTAAGCT GCCTGGAAGG 
ATGAGTCTGT TTACCCTAAA GAAAAATGCA CTTCAGTGGA CAAAGAGCCC ATCAGCGAAT 
GAGTCTGGAG ATGGGACACC AACAGCATCC ACCACATCAA CAGTTGAAGG AGCAGAGCAG 
GAAAGCTGCG ACTCCACAGA GACGGCTGCT GCTAGTGGTG AAAATGATGC CTCTGTGGAT 
GAGACTTCCT CCAGTGCCTC TTGCTCTACA GAACCACAGA CAAGACTCAG TCGCTCTTCA 
CAGTCAGGGA GACAGAGGAA GAAGGCTGTG ATGATGCCCC GTGTAGTTCT CACTCCTCTC 
AAGGTCAACG GTGAACACGT CCCATCAGGT CCCATGAAAA GGAGTCGAGG AGGAGTGGAG 
GTGGATTTTG AGACTCCAGG ATCCATCCTG GTCAACACCA ACATTCGTGC TCTCATCAAC 
ACACGGACCT TTGCTGCTTT TCCTTCTCAC TCCCAGCAGC AACTACTCCA GCTTTTACCA 
GAGGTGGACA GACAGGTCGG CCCCGATGGC CTTGCTCGTC TAAGCAGTTC GGCCCTCAAT 
AATGAATTCT TCACTCATGC CTCCCAGAGC TGGAAAGAAC GTCTAGCTGA GGGTGAGTTT 
ACACATGAGA TGCAGGTTCG CTTCAGGCAG GAGATGGAAA AGGAGAAGAA AGTAGAAGCA 
TGGAAGGAGA AATTCTTCGA GGAGTACCAT GGCCAAAGGT CTGGCTTAAC AAGAGAGGAG 
GCATTGAAGC TCACAATGAG CGAAGCTGGA GATGTTGCTA GTCCAGTGCT AGACTCCGAT 
TCTGCTACAG TGGCTACACC CAAGAGGCGA AGTGTTGGAA GAAGACGGAG AGAAGGCCGT 
ATCAGACGAC GTTCACGGGC TGACCTCAGG CGCAGGGCAA GGCGCACACT TTGCAAAACT 
ACAACTGCAA CTGTGGCTAC AGAAGAAACA ACAGAGAGCA CTGCAACTCC AGAAGAGGTA 
CCATCAGTTG CATCACCAGT CCCAGAAACC ACAGCAGTTC AAGGAGAGGT GGTCCTCCAA 
ACAGACCCTG AACTTGAAGC ACCAGAAGAA AACCTCTCTA CAGAGGCAGT ACCTTCTCCA 
GTTCCTGTAC CCACACCTCC TCCAGCATCG ACAAGCTCAA CCTGCGATGA GCCAGAAACC 
TCTACACGCC TGTTGCCAGA AGTACCCGAA CCAGCTGTTG CATCCACCTC ATCTCCTTCC 
TCATCCTCCT CTTCAACATC GTCATCATCT TCACCATCTT CCTCTCCATC CTCTGCCTCA 
GACAGACAGA CTTTTGCTGC AAGCTCAGAC TCCTCTTCTT CCTCTTCCTC CTCCACTGCT 
GCTGTTGCCA CTGATCCGCT GGATGATGGG GCTTCAGTCA TTACCACTGG CACAGCAGGC 
ACTGGAGCAA GCAGCAGAGA AAGCAGCCCT GCAGCCAGTC CCGCTACATC AAGCCCAGCA 
ATTCAGCTCA AGGAACAGAA GAGAAGACCA GACGAGTCCC AGGCCTTCAC CAGCTTTCCC 
GAGAAAAGGG CGCGGCTGGA TGAGCGTCAG TCCTTTCGTA ACACAGTTGA CTGTGTGCAC 
TCAGAAAAGC CACAGCCTAC AACAGAGGAG CCCAAGGTCC CGCCAATCCG GATACAGCTC 
TCCCGGATCA AACCGCCCTG GGTCAAGGGG CCGCCAACGT ACCAAATCTG TCCCCGCATC 
GTCCCCCCCA ACGAAGGGTC GCGGCGCTCT GGGACGGGGG CGCGCACCTT GGCGGACATC 
AAAGCCCGTG CACAGCAAGC CCGTGCCCAG CGGGAAGCCG CTGCTGCTGT TGCAGCCTCT 
GGGGATGGGG GAGGGCCTGG GGGGAGCGGC CCGGGGGGTG GTACTGGGAT ACCGGATCGC 
TCCAGCGGGA GGCGTACAAG AGAGCACCCA GGTCCCGTTG AACCTGGAGG AGGAGGAGGG 
AGACGAGGAG GAAGAGTTGA TTTGGAGGAG CAGGAATCGC CTGCGAGCTC TCATTCGTCG 
GGAGCACAAC TACAGCTGTC TAGTGTAGAT TCGGCAGACA GGCCTCAAAC TTCCACCCCA 
CTCACTGAAC CATCTCCCTC TTCTGTGTCC TCAAACCCAT CTCTACCATC ATCAGAATCT 
CCAAAAATAC TTGCGCTATC ACCACCTGCA ACCGACAGCC CTGCTTCCCA GGATCAGGTG 
GAGGAGATTT GTGGAGTGGA AGAGGTGACT GCCTGCCCAA GTGATAAAAC CTCAGAGCAG 
ACCTTAGACA CCCCAGTGCC TACCCCAAGT GAATCAGAGT CTTTTTGCAG TCACCAAGAT 
GGGAGAGGCG AGGAAGCAGA GTCGAGTGAT AGTAGAACAA ATACGCCTGT TTGCACCTCA 
TCATCAAATG ATGCTATTTC TTTAGTGCCC ACCTCTATAC CTGACTCGCT ACCAAGATTT 
GGTGCCCAGG GTGTGGATGT CATCAGGACA TTGGCAGCTT CCTCTCAGCC TTGGGAAGGT 
GAAAAGAAAG CGGTTGAGCT TCACCCAGGG ACAACTGGAG TTATCCAGCA TGGCTCAGAC 
TTGAAATTAC CCAAAGAGAC TCTTGTTACA GCCAGAAATG GATGGGTAGA AAATAGAGAA 
GAGCATTCCA TGAGAGAGGG TATGCTAGAA GAACATAGGA ATGGAGGGAG TGATGCTAAT 
GGTAAATATG AATCAGGCTC TTTACCTTGC CTACCACGAT GTGCTGGTGA GGAAGACACT 
GGAGCACACA GTGATTCAAC GGAGACAGCA TCTGACTTTG AAAATGAATC ACAAGAGGAT 
GAAATGGTGG ATTGGCATGG GACCCAGATG GATGGTAGTG GCATGCAAAC TCAAAATACA 
AAATCACTGA ATCGACCTGT TATTCAGACC TCAAGTCGAC TCACTTCATC GATTGTTAAT 
CCTCCTCAAC ACCAGCAACC GGTTATACAG GCCCATATAT CTAATCAAAA CCATACCCAA 
ACTGTTATTC AGGCTTGCTT TCCTAATGGT GTGCCAAACC AACCAGTCAT TCATCCTCAC 
AAGCAGCATG CAATTAATAC CCATGCTATA AACCACTCTC AGGACCAGAT TGCCTCTGCA 
CCTTCACAAG CCCAAGTGCA AGCTTATCTG ACCCAGACTG AGATGGACCA AAGCAGAAAT 
CTACGACTGA ATGATAATGG CAATGGTGTG AAGCTGTATT TGTCAGAGGA TGACACCAAA 
CCTCTTGGCA GGGAAGATTT AGTTTTAAAG AATTCTGCTG GTCCTCCAGT TGGCAGAAGA 
GTCCAAGTTT CATCTCGACC AGTTTCCACT GTAGAGGCCA ACAATCCTCT TGTCACACAG 
CTCCTCCAAG GCAGTCTACC TTTAGAGAAG GTCTTACCCC AACCCCACTC AGCCAGCAAG 
TTAGAAATTA ATCGACTTCC AGGTGCCAAT TCAAGCAACT CGCTCAACCC AGCCAATCAT 
CAAACACAGA GAAATCTGGC ACCACGTTTT AGAGGCCCAC CTGAGTCGGG AGGGCCTACG 
GACACTGTTA GTTCTGACTT CCAACACAAA ACCCCACCTG CTCGAGTATC TCCTGGACCT 
GGTCGGAACT TTGGTTCTTC CCCGCCAGGT TCTGCTCCAT CAAGAATGGC ATGCCTGTTT 
GAGGAAGCCA GCCCCAGGTC TGCAAACTCT CAGTTCAGTT CTCAGCAACC AGGAGGCGTT 
GTGCCTCCAG GAGCAGTACC TGTCATAACA TCCCTTCCCT TAAACTCCAC CACTAGAACC 
TCAATGGATA TGAACTCTCA GAATGCTCCA GTTTATGAAT CAGCTGTTAT TAAAGAGCAC 
CCTGGACCTC TTTCTCAACG GAGTGAAACT CCTGAGAGGC CAAGTAGCTT TCAGCAGCAT 
CCTAATAATG TCTCCCAGTC CGTGTGCAGA AACACACCAG ATGCTCCTTC ACCTCCACAT 
GGAGACCTTT GTCCATCAGA GGTTGTACCA ACTGTTAAGA TTAACTGGCG CCCAAACAAA 
CCTCAGCAAC CGATCTCCCC TGTAGCTACA GTAAAAAACG AAGTCTCCTT GCGCCCTTCG 
TGCCAACAAG CTCTGACCAA AAACTCACCT GCTCCTATCA GTGGCAATAC GTCTGTTGTA 
ATAACCAAAA AGGAGCCTCT GGATGGTTTT CATGGAGGTG GTGGAGCTAT GGAAGGACTG 
CTAAATATGG AAATGTCTCT TGTCAGAATG GCCAAGAAGG AACAAGTCAA AAATCCATAT 
ACCCGCCAAG CAGACACCTC CGCTTCTCCT GTTTCTTCCT CTCCTTCCTC ATCAGCCTCC 
AGTCTCCCTT TCCAGCTATA TGGTAAGCTA CCAAAACTTC AGCAGAGTGG GGGCAACGGA 
GGGTCCTCAT CCAGCTTCAG TTACACAGCT AATGTGTCTG TGGTGGATGG TAGTGGCTTC 
ACTCGCACCT TAGCCGATAG TGTGCTCCAA CTAAGGCCAC GTGTGAGCGT CGGCAGCGGT 
GGAGGTCAGA GTGCCACGCT TGGCATCCAG GCATTTACAG ACAGTGCTGC CGAAGAAGTG 
GCTCTCAAGT GTTCCTGCCG ACTCAAGGCT ATGATCATGT GCCAGGGCTG TGGCGCTTTC 
TGCCATGATG ATTGCATCGG CCCTTCGAAA CTGTGTGTGT CATGTCTGGT TGTCAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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asxl1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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