araf cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	araf
Entrez Gene ID	403063
Full Name	A-Raf proto-oncogene, serine/threonine kinase
Synonyms	sb:eu994,wu:fk45h07,zgc:92074
General protein information	Preferred Names A-Raf proto-oncogene, serine/threonine kinase Names serine/threonine-protein kinase A-Raf serine/threonine protein kinase ARAF alt1 serine/threonine protein kinase ARAF alt2 serine/threonine protein kinase ARAF alt3 serine/threonine protein kinase ARAF alt4 serine/threonine protein kinase ARAF alt5 serine/threonine protein kinase ARAF standard form truncated ARAF protein v-raf murine sarcoma 3611 viral oncogene homolog
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005171817.3	XP_005171874.1	serine/threonine-protein kinase A-Raf isoform X1
XM_005171818.3	XP_005171875.1	serine/threonine-protein kinase A-Raf isoform X2
XM_005171817.4	XP_005171874.1	serine/threonine-protein kinase A-Raf isoform X1
XM_005171818.4	XP_005171875.1	serine/threonine-protein kinase A-Raf isoform X2
NM_205743.1	NP_991306.1	serine/threonine-protein kinase A-Raf

Pathways from BioSystems

Homologies

Homo sapiens (human)	ARAF	NP_001645.1
Canis lupus familiaris (dog)	ARAF	XP_548976.3
Danio rerio (zebrafish)	araf	NP_991306.1
Mus musculus (house mouse)	Araf	NP_033833.1
Macaca mulatta (Rhesus monkey)	ARAF	XP_001095044.2
Bos taurus (cattle)	ARAF	NP_001014964.1
Rattus norvegicus (Norway rat)	Araf	NP_071977.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

The Kinase Activity-deficient Isoform of the Protein Araf Antagonizes Ras/Mitogen-activated Protein Kinase (Ras/MAPK) Signaling in the Zebrafish Embryo.
Xiong C, Liu X, Meng A
The Journal of biological chemistry290(42)25512-21(2015 Oct)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Araf kinase antagonizes Nodal-Smad2 activity in mesendoderm development by directly phosphorylating the Smad2 linker region.
Liu X, Xiong C, Jia S, Zhang Y, Chen YG, Wang Q, Meng A
Nature communications41728(2013)

Temporal pattern of loss/persistence of duplicate genes involved in signal transduction and metabolic pathways after teleost-specific genome duplication.
Sato Y, Hashiguchi Y, Nishida M
BMC evolutionary biology9127(2009 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following araf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the araf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa35126	NM_205743.1 Latest version!	Danio rerio A-Raf proto-oncogene, serine/threonine kinase (araf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODa10444	XM_005171817.4 Latest version!	Danio rerio A-Raf proto-oncogene, serine/threonine kinase (araf), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODa10445	XM_005171818.4 Latest version!	Danio rerio A-Raf proto-oncogene, serine/threonine kinase (araf), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
ODa10444	XM_005171817.3	Danio rerio A-Raf proto-oncogene, serine/threonine kinase (araf), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODa10445	XM_005171818.3	Danio rerio A-Raf proto-oncogene, serine/threonine kinase (araf), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

araf ( NM_205743.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_991306.1 Danio rerio A-Raf proto-oncogene, serine/threonine kinase (araf), mRNA.

Price & Availability↑

CloneID	ODa35126
Clone ID Related Accession (Same CDS sequence)	NM_205743.1
Accession Version	NM_205743.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1542902400000
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase A-Raf
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB120005.1. ##Evidence-Data-START## Transcript exon combination :: AB120005.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTCCAACA CTTCCTCCTG TTCCTCCTCG GGGGAAACCA GCCCGGACGA TACACCCCGA 
GGTGGAGGCA CCATCCGCGT CTACCTCCCT AACAAGCAGC GGACGGTGGT AAACGTTCGG 
CCTGGGCAAA CTGTGTATGA TAGTTTAGAT AAAGCCCTAA AAGTCCGAGG CCTAAGCCAG 
GATTGCTGTG CTGTCTTTCG CCTGCTTGAA GGCCGGAAGA GACTTACAGA ATGGGACACT 
GACATCACAC CCCTCGTGGG GGAAGAGCTA CTGGTAGAAG TCCTGGATGA TGTTCCCCTT 
ACCATGCACA ACTTTGTACG TAAAACGTTC TTTAAGTTAG CATACTGTGA CTTCTGCCAC 
AAGTTCTTAT TCAACGGCTT CAGGTGTCAG ACATGTGGAT ATAAATTTCA CCAGCACTGC 
AGCAGTAAAG TCCCTACAGT ATGTGTGGAC ATGGACACAA TGACAAAACG GTGTGTTAAT 
AATACAGAAG ACTGCCCTGC GATATTGATA TATCCGGCCA CCAATTCCAT ATCGCAGAGC 
GACCTACCTT TAACCTCAGA TTCTCCTGGA GGGGAGCTGC CGTCTTCCCC GTCAAACTTC 
TGTTTGTTCA TTCGAGGAGA GGACGGTCAG TCTCTCCAGA GGCATCGATC AACCTCAACA 
CCCAATGTTC ACATGGTCAG CACGCTGGAG CCTGGCGCTG CTGGCCTTTT GGAGGAGCCA 
TTCAAAATCA GCACAGTGGC GTCCTACACA GGGTACGAGT CCTCTCCCAA ACCTTCCACT 
AGCCCACCCC ACCTAGGGTC GCCAGGAAGA AAACCCCCCA AATCCCCTTC TGAACCCAAG 
GAACGAAAGC AGTCCTCGTC TGATGATAAA AAGAAAGTGC ACCGGGGAGG TTACAGAGAC 
TCCAGTTACT ACTGGGAGGT GCACCCGCGG GAGGTGTCCA TGTTAAAGAG GATTGGAGCA 
GGCTCTTTTG GGACCGTCTT TAAAGGCAAG TGGCATGGAG ACGTGGCCAT CAAAATTCTT 
AAAGTGACAG AGCCCACACC GGAGCAGCTC CAGGCCTTTA AAAATGAGAT GCAGGTTTTA 
CGAAAAACAC GCCATGTAAA CATTCTGCTG TTTATGGGCT TCATGACAAG GCCTAATTTT 
GCCATTATCA CACAGTGGTG TGAAGGCAGC AGTCTGTATC GCCATCTGCA TGTCACTGAG 
ACCAAGTTTG ACACCATGCG GCGCATTGAT GTTGCTCGCC AAACAGCACA GGGCATGGAT 
TATCTTCACG CCAAGAATAT CATTCATCGG GACTTGAAAT CTAATAATAT CTTCCTGCAT 
GAAGGCTGGA CAGTAAAAAT CGGTGACTTT GGCTTGGCTA CAGTAAAGTC TCGCTGGAGT 
GGCTCACAGC AGGTCGAACA GCCCAGTGGC TCCATCCTGT GGATGGCTCC TGAGGTGATC 
AGAATGCAAG ACACTAACCC GTACACCTTC CAGTCTGATG TGTATGGATA TGGAGTGGTG 
CTCTACGAAC TGATGTCTGG GACTTTGCCT TATTCGAATA TCAACAACCG CGATCAGATT 
ATTTTCATGG TGGGTCGTGG TTACTTGTCT CCAGACCTCA GTAAATTATA CAGTAACTGC 
CCGAAATCAA TGAAGAGGCT CATCATTGAC TGTCTGAAAT TCAAACGGGA TGAACGGCCT 
CTCTTTCCAC AGATCCTGGT GAGCATTGAG CAGGTCCAGG AATTGTTGCC AAAAATCGAG 
CGGAGTGCCT CTGAGCCCTC TCTACACCGT GCTGTACATG CCGAGGACCT GAATCCGCTT 
CTCCTTCACA CCACGCGCTT CCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_991306.1
CDS	122..1948
Translation	MSNTSSCSSSGETSPDDTPRGGGTIRVYLPNKQRTVVNVRPGQTVYDSLDKALKVRGLSQDCCAVFRLLEGRKRLTEWDTDITPLVGEELLVEVLDDVPLTMHNFVRKTFFKLAYCDFCHKFLFNGFRCQTCGYKFHQHCSSKVPTVCVDMDTMTKRCVNNTEDCPAILIYPATNSISQSDLPLTSDSPGGELPSSPSNFCLFIRGEDGQSLQRHRSTSTPNVHMVSTLEPGAAGLLEEPFKISTVASYTGYESSPKPSTSPPHLGSPGRKPPKSPSEPKERKQSSSDDKKKVHRGGYRDSSYYWEVHPREVSMLKRIGAGSFGTVFKGKWHGDVAIKILKVTEPTPEQLQAFKNEMQVLRKTRHVNILLFMGFMTRPNFAIITQWCEGSSLYRHLHVTETKFDTMRRIDVARQTAQGMDYLHAKNIIHRDLKSNNIFLHEGWTVKIGDFGLATVKSRWSGSQQVEQPSGSILWMAPEVIRMQDTNPYTFQSDVYGYGVVLYELMSGTLPYSNINNRDQIIFMVGRGYLSPDLSKLYSNCPKSMKRLIIDCLKFKRDERPLFPQILVSIEQVQELLPKIERSASEPSLHRAVHAEDLNPLLLHTTRFL

Target ORF information:

RefSeq Version	NM_205743.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio A-Raf proto-oncogene, serine/threonine kinase (araf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205743.1

ORF Insert Sequence:

ATGTCCAACA CTTCCTCCTG TTCCTCCTCG GGGGAAACCA GCCCGGACGA TACACCCCGA 
GGTGGAGGCA CCATCCGCGT CTACCTCCCT AACAAGCAGC GGACGGTGGT AAACGTTCGG 
CCTGGGCAAA CTGTGTATGA TAGTTTAGAT AAAGCCCTAA AAGTCCGAGG CCTAAGCCAG 
GATTGCTGTG CTGTCTTTCG CCTGCTTGAA GGCCGGAAGA GACTTACAGA ATGGGACACT 
GACATCACAC CCCTCGTGGG GGAAGAGCTA CTGGTAGAAG TCCTGGATGA TGTTCCCCTT 
ACCATGCACA ACTTTGTACG TAAAACGTTC TTTAAGTTAG CATACTGTGA CTTCTGCCAC 
AAGTTCTTAT TCAACGGCTT CAGGTGTCAG ACATGTGGAT ATAAATTTCA CCAGCACTGC 
AGCAGTAAAG TCCCTACAGT ATGTGTGGAC ATGGACACAA TGACAAAACG GTGTGTTAAT 
AATACAGAAG ACTGCCCTGC GATATTGATA TATCCGGCCA CCAATTCCAT ATCGCAGAGC 
GACCTACCTT TAACCTCAGA TTCTCCTGGA GGGGAGCTGC CGTCTTCCCC GTCAAACTTC 
TGTTTGTTCA TTCGAGGAGA GGACGGTCAG TCTCTCCAGA GGCATCGATC AACCTCAACA 
CCCAATGTTC ACATGGTCAG CACGCTGGAG CCTGGCGCTG CTGGCCTTTT GGAGGAGCCA 
TTCAAAATCA GCACAGTGGC GTCCTACACA GGGTACGAGT CCTCTCCCAA ACCTTCCACT 
AGCCCACCCC ACCTAGGGTC GCCAGGAAGA AAACCCCCCA AATCCCCTTC TGAACCCAAG 
GAACGAAAGC AGTCCTCGTC TGATGATAAA AAGAAAGTGC ACCGGGGAGG TTACAGAGAC 
TCCAGTTACT ACTGGGAGGT GCACCCGCGG GAGGTGTCCA TGTTAAAGAG GATTGGAGCA 
GGCTCTTTTG GGACCGTCTT TAAAGGCAAG TGGCATGGAG ACGTGGCCAT CAAAATTCTT 
AAAGTGACAG AGCCCACACC GGAGCAGCTC CAGGCCTTTA AAAATGAGAT GCAGGTTTTA 
CGAAAAACAC GCCATGTAAA CATTCTGCTG TTTATGGGCT TCATGACAAG GCCTAATTTT 
GCCATTATCA CACAGTGGTG TGAAGGCAGC AGTCTGTATC GCCATCTGCA TGTCACTGAG 
ACCAAGTTTG ACACCATGCG GCGCATTGAT GTTGCTCGCC AAACAGCACA GGGCATGGAT 
TATCTTCACG CCAAGAATAT CATTCATCGG GACTTGAAAT CTAATAATAT CTTCCTGCAT 
GAAGGCTGGA CAGTAAAAAT CGGTGACTTT GGCTTGGCTA CAGTAAAGTC TCGCTGGAGT 
GGCTCACAGC AGGTCGAACA GCCCAGTGGC TCCATCCTGT GGATGGCTCC TGAGGTGATC 
AGAATGCAAG ACACTAACCC GTACACCTTC CAGTCTGATG TGTATGGATA TGGAGTGGTG 
CTCTACGAAC TGATGTCTGG GACTTTGCCT TATTCGAATA TCAACAACCG CGATCAGATT 
ATTTTCATGG TGGGTCGTGG TTACTTGTCT CCAGACCTCA GTAAATTATA CAGTAACTGC 
CCGAAATCAA TGAAGAGGCT CATCATTGAC TGTCTGAAAT TCAAACGGGA TGAACGGCCT 
CTCTTTCCAC AGATCCTGGT GAGCATTGAG CAGGTCCAGG AATTGTTGCC AAAAATCGAG 
CGGAGTGCCT CTGAGCCCTC TCTACACCGT GCTGTACATG CCGAGGACCT GAATCCGCTT 
CTCCTTCACA CCACGCGCTT CCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

araf ( XM_005171817.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171874.1 Danio rerio A-Raf proto-oncogene, serine/threonine kinase (araf), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa10444
Clone ID Related Accession (Same CDS sequence)	XM_005171817.3 , XM_005171817.4
Accession Version	XM_005171817.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase A-Raf isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005171817.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACA CTTCCTCCTG TTCCTCCTCG GGGGAAACCA GCCCGGACGA TACACCCCGA 
GGTGGAGGCA CCATCCGCGT CTACCTCCCT AACAAGCAGC GGACGGTGGT AAACGTTCGG 
CCTGGGCAAA CTGTGTATGA TAGTTTAGAT AAAGCCCTAA AAGTCCGAGG CCTAAGCCAG 
GATTGCTGTG CTGTCTTTCG CCTGCTTGAA GGCCGGAAGA GACTTACAGA ATGGGACACT 
GACATCACAC CCCTCGTGGG GGAAGAGCTA CTGGTAGAAG TCCTGGATGA TGTTCCCCTT 
ACCATGCACA ACTTTGTACG TAAAACGTTC TTTAAGTTAG CATACTGTGA CTTCTGCCAC 
AAGTTCTTAT TCAACGGCTT CAGGTGTCAG ACATGTGGAT ATAAATTTCA CCAGCACTGC 
AGCAGTAAAG TCCCTACAGT ATGTGTGGAC ATGGACACAA TGACAAAACG GTGTGTTAAT 
AATACAGAAG ACTGCCCTGC GATATTGATA TATCCGGCCA CCAATTCCAT ATCGCAGAGC 
GACCTACCTT TAACCTCAGA TTCTCCTGGA GGGGAGCTGC CGTCTTCCCC GTCAAACTTC 
TGTTTGTTCA TTCGAGGAGA GGACGGTCAG TCTCTCCAGA GGCATCGATC AACCTCAACA 
CCCAATGTTC ACATGGTCAG CACGCTGGAG CCTGGCGCTG CTGGCCTTTT GGAGGAGCCA 
TTCAAAATCA GCACAGTGGC GTCGTCCACA GGGTACGAGT CCTCTCCCAA ACCTTCCACT 
AGCCCACCCC ACCTAGGGTC GCCAGGAAGA AAACCCCCCA AATCCCCTTC TGAACCCAAG 
GAACGAAAGC AGTCCTCGTC TGATGATAAA AAGAAAGTGC ACCGGGGAGG TTACAGAGAC 
TCCAGTTACT ACTGGGAGGT GCACCCGCGG GAGGTGTCCA TGTTAAAGAG GATTGGAGCA 
GGCTCTTTTG GGACCGTCTT TAAAGGCAAG TGGCATGGAG ACGTGGCCAT CAAAATTCTT 
AAAGTGACAG AGCCCACACC GGAGCAGCTC CAGGCCTTTA AAAATGAGAT GCAGGTTTTA 
CGAAAAACAC GCCATGTAAA CATTCTGCTG TTTATGGGCT TCATGACAAG GCCTAATTTT 
GCCATTATCA CACAGTGGTG TGAAGGCAGC AGTCTGTATC GCCATCTGCA TGTCACTGAG 
ACCAAGTTTG ACACCATGCG GCGCATTGAT GTTGCTCGCC AAACAGCACA GGGCATGGAT 
TATCTTCACG CCAAGAATAT CATTCATCGG GACTTGAAAT CTAATAATAT CTTCCTGCAT 
GAAGGCTGGA CAGTAAAAAT CGGTGACTTT GGCTTGGCTA CAGTAAAGTC TCGCTGGAGT 
GGCTCACAGC AGGTCGAACA GCCCAGTGGC TCCATCCTGT GGATGGCTCC TGAGGTGATC 
AGAATGCAAG ACACTAACCC GTACACCTTC CAGTCTGATG TGTATGGATA TGGAGTGGTG 
CTCTACGAAC TGATGTCTGG GACTTTGCCT TATTCGAATA TCAACAACCG CGATCAGATT 
ATTTTCATGG TGGGTCGTGG TTACTTGTCT CCAGACCTCA GTAAATTATA CAGTAACTGC 
CCGAAATCAA TGAAGAGGCT CATCATTGAC TGTCTGAAAT TCAAACGGGA TGAACGGCCT 
CTCTTCCCAC AGATCCTGGT GAGCATTGAG CAGGTCCAGG AATTGTTGCC AAAAATCGAG 
CGGAGTGCCT CTGAGCCCTC TCTACACCGT GCTGTACATG CCGAGGACCT GAATCCGCTT 
CTCCTTCACA CCACGCGCTT CCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171874.1
CDS	708..2534
Translation	MSNTSSCSSSGETSPDDTPRGGGTIRVYLPNKQRTVVNVRPGQTVYDSLDKALKVRGLSQDCCAVFRLLEGRKRLTEWDTDITPLVGEELLVEVLDDVPLTMHNFVRKTFFKLAYCDFCHKFLFNGFRCQTCGYKFHQHCSSKVPTVCVDMDTMTKRCVNNTEDCPAILIYPATNSISQSDLPLTSDSPGGELPSSPSNFCLFIRGEDGQSLQRHRSTSTPNVHMVSTLEPGAAGLLEEPFKISTVASSTGYESSPKPSTSPPHLGSPGRKPPKSPSEPKERKQSSSDDKKKVHRGGYRDSSYYWEVHPREVSMLKRIGAGSFGTVFKGKWHGDVAIKILKVTEPTPEQLQAFKNEMQVLRKTRHVNILLFMGFMTRPNFAIITQWCEGSSLYRHLHVTETKFDTMRRIDVARQTAQGMDYLHAKNIIHRDLKSNNIFLHEGWTVKIGDFGLATVKSRWSGSQQVEQPSGSILWMAPEVIRMQDTNPYTFQSDVYGYGVVLYELMSGTLPYSNINNRDQIIFMVGRGYLSPDLSKLYSNCPKSMKRLIIDCLKFKRDERPLFPQILVSIEQVQELLPKIERSASEPSLHRAVHAEDLNPLLLHTTRFL

Target ORF information:

RefSeq Version	XM_005171817.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio A-Raf proto-oncogene, serine/threonine kinase (araf), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171817.3

ORF Insert Sequence:

ATGTCCAACA CTTCCTCCTG TTCCTCCTCG GGGGAAACCA GCCCGGACGA TACACCCCGA 
GGTGGAGGCA CCATCCGCGT CTACCTCCCT AACAAGCAGC GGACGGTGGT AAACGTTCGG 
CCTGGGCAAA CTGTGTATGA TAGTTTAGAT AAAGCCCTAA AAGTCCGAGG CCTAAGCCAG 
GATTGCTGTG CTGTCTTTCG CCTGCTTGAA GGCCGGAAGA GACTTACAGA ATGGGACACT 
GACATCACAC CCCTCGTGGG GGAAGAGCTA CTGGTAGAAG TCCTGGATGA TGTTCCCCTT 
ACCATGCACA ACTTTGTACG TAAAACGTTC TTTAAGTTAG CATACTGTGA CTTCTGCCAC 
AAGTTCTTAT TCAACGGCTT CAGGTGTCAG ACATGTGGAT ATAAATTTCA CCAGCACTGC 
AGCAGTAAAG TCCCTACAGT ATGTGTGGAC ATGGACACAA TGACAAAACG GTGTGTTAAT 
AATACAGAAG ACTGCCCTGC GATATTGATA TATCCGGCCA CCAATTCCAT ATCGCAGAGC 
GACCTACCTT TAACCTCAGA TTCTCCTGGA GGGGAGCTGC CGTCTTCCCC GTCAAACTTC 
TGTTTGTTCA TTCGAGGAGA GGACGGTCAG TCTCTCCAGA GGCATCGATC AACCTCAACA 
CCCAATGTTC ACATGGTCAG CACGCTGGAG CCTGGCGCTG CTGGCCTTTT GGAGGAGCCA 
TTCAAAATCA GCACAGTGGC GTCGTCCACA GGGTACGAGT CCTCTCCCAA ACCTTCCACT 
AGCCCACCCC ACCTAGGGTC GCCAGGAAGA AAACCCCCCA AATCCCCTTC TGAACCCAAG 
GAACGAAAGC AGTCCTCGTC TGATGATAAA AAGAAAGTGC ACCGGGGAGG TTACAGAGAC 
TCCAGTTACT ACTGGGAGGT GCACCCGCGG GAGGTGTCCA TGTTAAAGAG GATTGGAGCA 
GGCTCTTTTG GGACCGTCTT TAAAGGCAAG TGGCATGGAG ACGTGGCCAT CAAAATTCTT 
AAAGTGACAG AGCCCACACC GGAGCAGCTC CAGGCCTTTA AAAATGAGAT GCAGGTTTTA 
CGAAAAACAC GCCATGTAAA CATTCTGCTG TTTATGGGCT TCATGACAAG GCCTAATTTT 
GCCATTATCA CACAGTGGTG TGAAGGCAGC AGTCTGTATC GCCATCTGCA TGTCACTGAG 
ACCAAGTTTG ACACCATGCG GCGCATTGAT GTTGCTCGCC AAACAGCACA GGGCATGGAT 
TATCTTCACG CCAAGAATAT CATTCATCGG GACTTGAAAT CTAATAATAT CTTCCTGCAT 
GAAGGCTGGA CAGTAAAAAT CGGTGACTTT GGCTTGGCTA CAGTAAAGTC TCGCTGGAGT 
GGCTCACAGC AGGTCGAACA GCCCAGTGGC TCCATCCTGT GGATGGCTCC TGAGGTGATC 
AGAATGCAAG ACACTAACCC GTACACCTTC CAGTCTGATG TGTATGGATA TGGAGTGGTG 
CTCTACGAAC TGATGTCTGG GACTTTGCCT TATTCGAATA TCAACAACCG CGATCAGATT 
ATTTTCATGG TGGGTCGTGG TTACTTGTCT CCAGACCTCA GTAAATTATA CAGTAACTGC 
CCGAAATCAA TGAAGAGGCT CATCATTGAC TGTCTGAAAT TCAAACGGGA TGAACGGCCT 
CTCTTCCCAC AGATCCTGGT GAGCATTGAG CAGGTCCAGG AATTGTTGCC AAAAATCGAG 
CGGAGTGCCT CTGAGCCCTC TCTACACCGT GCTGTACATG CCGAGGACCT GAATCCGCTT 
CTCCTTCACA CCACGCGCTT CCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

araf ( XM_005171817.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171874.1 Danio rerio A-Raf proto-oncogene, serine/threonine kinase (araf), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa10444
Clone ID Related Accession (Same CDS sequence)	XM_005171817.3 , XM_005171817.4
Accession Version	XM_005171817.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase A-Raf isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005171817.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACA CTTCCTCCTG TTCCTCCTCG GGGGAAACCA GCCCGGACGA TACACCCCGA 
GGTGGAGGCA CCATCCGCGT CTACCTCCCT AACAAGCAGC GGACGGTGGT AAACGTTCGG 
CCTGGGCAAA CTGTGTATGA TAGTTTAGAT AAAGCCCTAA AAGTCCGAGG CCTAAGCCAG 
GATTGCTGTG CTGTCTTTCG CCTGCTTGAA GGCCGGAAGA GACTTACAGA ATGGGACACT 
GACATCACAC CCCTCGTGGG GGAAGAGCTA CTGGTAGAAG TCCTGGATGA TGTTCCCCTT 
ACCATGCACA ACTTTGTACG TAAAACGTTC TTTAAGTTAG CATACTGTGA CTTCTGCCAC 
AAGTTCTTAT TCAACGGCTT CAGGTGTCAG ACATGTGGAT ATAAATTTCA CCAGCACTGC 
AGCAGTAAAG TCCCTACAGT ATGTGTGGAC ATGGACACAA TGACAAAACG GTGTGTTAAT 
AATACAGAAG ACTGCCCTGC GATATTGATA TATCCGGCCA CCAATTCCAT ATCGCAGAGC 
GACCTACCTT TAACCTCAGA TTCTCCTGGA GGGGAGCTGC CGTCTTCCCC GTCAAACTTC 
TGTTTGTTCA TTCGAGGAGA GGACGGTCAG TCTCTCCAGA GGCATCGATC AACCTCAACA 
CCCAATGTTC ACATGGTCAG CACGCTGGAG CCTGGCGCTG CTGGCCTTTT GGAGGAGCCA 
TTCAAAATCA GCACAGTGGC GTCGTCCACA GGGTACGAGT CCTCTCCCAA ACCTTCCACT 
AGCCCACCCC ACCTAGGGTC GCCAGGAAGA AAACCCCCCA AATCCCCTTC TGAACCCAAG 
GAACGAAAGC AGTCCTCGTC TGATGATAAA AAGAAAGTGC ACCGGGGAGG TTACAGAGAC 
TCCAGTTACT ACTGGGAGGT GCACCCGCGG GAGGTGTCCA TGTTAAAGAG GATTGGAGCA 
GGCTCTTTTG GGACCGTCTT TAAAGGCAAG TGGCATGGAG ACGTGGCCAT CAAAATTCTT 
AAAGTGACAG AGCCCACACC GGAGCAGCTC CAGGCCTTTA AAAATGAGAT GCAGGTTTTA 
CGAAAAACAC GCCATGTAAA CATTCTGCTG TTTATGGGCT TCATGACAAG GCCTAATTTT 
GCCATTATCA CACAGTGGTG TGAAGGCAGC AGTCTGTATC GCCATCTGCA TGTCACTGAG 
ACCAAGTTTG ACACCATGCG GCGCATTGAT GTTGCTCGCC AAACAGCACA GGGCATGGAT 
TATCTTCACG CCAAGAATAT CATTCATCGG GACTTGAAAT CTAATAATAT CTTCCTGCAT 
GAAGGCTGGA CAGTAAAAAT CGGTGACTTT GGCTTGGCTA CAGTAAAGTC TCGCTGGAGT 
GGCTCACAGC AGGTCGAACA GCCCAGTGGC TCCATCCTGT GGATGGCTCC TGAGGTGATC 
AGAATGCAAG ACACTAACCC GTACACCTTC CAGTCTGATG TGTATGGATA TGGAGTGGTG 
CTCTACGAAC TGATGTCTGG GACTTTGCCT TATTCGAATA TCAACAACCG CGATCAGATT 
ATTTTCATGG TGGGTCGTGG TTACTTGTCT CCAGACCTCA GTAAATTATA CAGTAACTGC 
CCGAAATCAA TGAAGAGGCT CATCATTGAC TGTCTGAAAT TCAAACGGGA TGAACGGCCT 
CTCTTCCCAC AGATCCTGGT GAGCATTGAG CAGGTCCAGG AATTGTTGCC AAAAATCGAG 
CGGAGTGCCT CTGAGCCCTC TCTACACCGT GCTGTACATG CCGAGGACCT GAATCCGCTT 
CTCCTTCACA CCACGCGCTT CCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171874.1
CDS	179..2005
Translation	MSNTSSCSSSGETSPDDTPRGGGTIRVYLPNKQRTVVNVRPGQTVYDSLDKALKVRGLSQDCCAVFRLLEGRKRLTEWDTDITPLVGEELLVEVLDDVPLTMHNFVRKTFFKLAYCDFCHKFLFNGFRCQTCGYKFHQHCSSKVPTVCVDMDTMTKRCVNNTEDCPAILIYPATNSISQSDLPLTSDSPGGELPSSPSNFCLFIRGEDGQSLQRHRSTSTPNVHMVSTLEPGAAGLLEEPFKISTVASSTGYESSPKPSTSPPHLGSPGRKPPKSPSEPKERKQSSSDDKKKVHRGGYRDSSYYWEVHPREVSMLKRIGAGSFGTVFKGKWHGDVAIKILKVTEPTPEQLQAFKNEMQVLRKTRHVNILLFMGFMTRPNFAIITQWCEGSSLYRHLHVTETKFDTMRRIDVARQTAQGMDYLHAKNIIHRDLKSNNIFLHEGWTVKIGDFGLATVKSRWSGSQQVEQPSGSILWMAPEVIRMQDTNPYTFQSDVYGYGVVLYELMSGTLPYSNINNRDQIIFMVGRGYLSPDLSKLYSNCPKSMKRLIIDCLKFKRDERPLFPQILVSIEQVQELLPKIERSASEPSLHRAVHAEDLNPLLLHTTRFL

Target ORF information:

RefSeq Version	XM_005171817.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio A-Raf proto-oncogene, serine/threonine kinase (araf), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171817.4

ORF Insert Sequence:

ATGTCCAACA CTTCCTCCTG TTCCTCCTCG GGGGAAACCA GCCCGGACGA TACACCCCGA 
GGTGGAGGCA CCATCCGCGT CTACCTCCCT AACAAGCAGC GGACGGTGGT AAACGTTCGG 
CCTGGGCAAA CTGTGTATGA TAGTTTAGAT AAAGCCCTAA AAGTCCGAGG CCTAAGCCAG 
GATTGCTGTG CTGTCTTTCG CCTGCTTGAA GGCCGGAAGA GACTTACAGA ATGGGACACT 
GACATCACAC CCCTCGTGGG GGAAGAGCTA CTGGTAGAAG TCCTGGATGA TGTTCCCCTT 
ACCATGCACA ACTTTGTACG TAAAACGTTC TTTAAGTTAG CATACTGTGA CTTCTGCCAC 
AAGTTCTTAT TCAACGGCTT CAGGTGTCAG ACATGTGGAT ATAAATTTCA CCAGCACTGC 
AGCAGTAAAG TCCCTACAGT ATGTGTGGAC ATGGACACAA TGACAAAACG GTGTGTTAAT 
AATACAGAAG ACTGCCCTGC GATATTGATA TATCCGGCCA CCAATTCCAT ATCGCAGAGC 
GACCTACCTT TAACCTCAGA TTCTCCTGGA GGGGAGCTGC CGTCTTCCCC GTCAAACTTC 
TGTTTGTTCA TTCGAGGAGA GGACGGTCAG TCTCTCCAGA GGCATCGATC AACCTCAACA 
CCCAATGTTC ACATGGTCAG CACGCTGGAG CCTGGCGCTG CTGGCCTTTT GGAGGAGCCA 
TTCAAAATCA GCACAGTGGC GTCGTCCACA GGGTACGAGT CCTCTCCCAA ACCTTCCACT 
AGCCCACCCC ACCTAGGGTC GCCAGGAAGA AAACCCCCCA AATCCCCTTC TGAACCCAAG 
GAACGAAAGC AGTCCTCGTC TGATGATAAA AAGAAAGTGC ACCGGGGAGG TTACAGAGAC 
TCCAGTTACT ACTGGGAGGT GCACCCGCGG GAGGTGTCCA TGTTAAAGAG GATTGGAGCA 
GGCTCTTTTG GGACCGTCTT TAAAGGCAAG TGGCATGGAG ACGTGGCCAT CAAAATTCTT 
AAAGTGACAG AGCCCACACC GGAGCAGCTC CAGGCCTTTA AAAATGAGAT GCAGGTTTTA 
CGAAAAACAC GCCATGTAAA CATTCTGCTG TTTATGGGCT TCATGACAAG GCCTAATTTT 
GCCATTATCA CACAGTGGTG TGAAGGCAGC AGTCTGTATC GCCATCTGCA TGTCACTGAG 
ACCAAGTTTG ACACCATGCG GCGCATTGAT GTTGCTCGCC AAACAGCACA GGGCATGGAT 
TATCTTCACG CCAAGAATAT CATTCATCGG GACTTGAAAT CTAATAATAT CTTCCTGCAT 
GAAGGCTGGA CAGTAAAAAT CGGTGACTTT GGCTTGGCTA CAGTAAAGTC TCGCTGGAGT 
GGCTCACAGC AGGTCGAACA GCCCAGTGGC TCCATCCTGT GGATGGCTCC TGAGGTGATC 
AGAATGCAAG ACACTAACCC GTACACCTTC CAGTCTGATG TGTATGGATA TGGAGTGGTG 
CTCTACGAAC TGATGTCTGG GACTTTGCCT TATTCGAATA TCAACAACCG CGATCAGATT 
ATTTTCATGG TGGGTCGTGG TTACTTGTCT CCAGACCTCA GTAAATTATA CAGTAACTGC 
CCGAAATCAA TGAAGAGGCT CATCATTGAC TGTCTGAAAT TCAAACGGGA TGAACGGCCT 
CTCTTCCCAC AGATCCTGGT GAGCATTGAG CAGGTCCAGG AATTGTTGCC AAAAATCGAG 
CGGAGTGCCT CTGAGCCCTC TCTACACCGT GCTGTACATG CCGAGGACCT GAATCCGCTT 
CTCCTTCACA CCACGCGCTT CCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

araf ( XM_005171818.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171875.1 Danio rerio A-Raf proto-oncogene, serine/threonine kinase (araf), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa10445
Clone ID Related Accession (Same CDS sequence)	XM_005171818.3 , XM_005171818.4
Accession Version	XM_005171818.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase A-Raf isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005171818.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACA CTTCCTCCTG TTCCTCCTCG GGGGAAACCA GCCCGGACGA TACACCCCGA 
GGTGGAGGCA CCATCCGCGT CTACCTCCCT AACAAGCAGC GGACGGTGGT AAACGTTCGG 
CCTGGGCAAA CTGTGTATGA TAGTTTAGAT AAAGCCCTAA AAGTCCGAGG CCTAAGCCAG 
GATTGCTGTG CTGTCTTTCG CCTGCTTGAA GGCCGGAAGA GACTTACAGA ATGGGACACT 
GACATCACAC CCCTCGTGGG GGAAGAGCTA CTGGTAGAAG TCCTGGATGA TGTTCCCCTT 
ACCATGCACA ACTTTGTACG TAAAACGTTC TTTAAGTTAG CATACTGTGA CTTCTGCCAC 
AAGTTCTTAT TCAACGGCTT CAGGTGTCAG ACATGTGGAT ATAAATTTCA CCAGCACTGC 
AGCAGTAAAG TCCCTACAGT ATGTGTGGAC ATGGACACAA TGACAAAACG GTGTGTTAAT 
AATACAGAAG ACTGCCCTGC GATATTGATA TATCCGGCCA CCAATTCCAT ATCGCAGAGC 
GACCTACCTT TAACCTCAGA TTCTCCTGGA GGGGAGCTGC CGTCTTCCCC GTCAAACTTC 
TGTTTGTTCA TTCGAGGAGA GGACGGTCAG TCTCTCCAGA GGCATCGATC AACCTCAACA 
CCCAATGTTC ACATGGTCAG CACGCTGGAG CCTGGCGCTG CTGGCCTTTT GGAGGAGCCA 
TTCAAAATCA GCACAGTGGG GTACGAGTCC TCTCCCAAAC CTTCCACTAG CCCACCCCAC 
CTAGGGTCGC CAGGAAGAAA ACCCCCCAAA TCCCCTTCTG AACCCAAGGA ACGAAAGCAG 
TCCTCGTCTG ATGATAAAAA GAAAGTGCAC CGGGGAGGTT ACAGAGACTC CAGTTACTAC 
TGGGAGGTGC ACCCGCGGGA GGTGTCCATG TTAAAGAGGA TTGGAGCAGG CTCTTTTGGG 
ACCGTCTTTA AAGGCAAGTG GCATGGAGAC GTGGCCATCA AAATTCTTAA AGTGACAGAG 
CCCACACCGG AGCAGCTCCA GGCCTTTAAA AATGAGATGC AGGTTTTACG AAAAACACGC 
CATGTAAACA TTCTGCTGTT TATGGGCTTC ATGACAAGGC CTAATTTTGC CATTATCACA 
CAGTGGTGTG AAGGCAGCAG TCTGTATCGC CATCTGCATG TCACTGAGAC CAAGTTTGAC 
ACCATGCGGC GCATTGATGT TGCTCGCCAA ACAGCACAGG GCATGGATTA TCTTCACGCC 
AAGAATATCA TTCATCGGGA CTTGAAATCT AATAATATCT TCCTGCATGA AGGCTGGACA 
GTAAAAATCG GTGACTTTGG CTTGGCTACA GTAAAGTCTC GCTGGAGTGG CTCACAGCAG 
GTCGAACAGC CCAGTGGCTC CATCCTGTGG ATGGCTCCTG AGGTGATCAG AATGCAAGAC 
ACTAACCCGT ACACCTTCCA GTCTGATGTG TATGGATATG GAGTGGTGCT CTACGAACTG 
ATGTCTGGGA CTTTGCCTTA TTCGAATATC AACAACCGCG ATCAGATTAT TTTCATGGTG 
GGTCGTGGTT ACTTGTCTCC AGACCTCAGT AAATTATACA GTAACTGCCC GAAATCAATG 
AAGAGGCTCA TCATTGACTG TCTGAAATTC AAACGGGATG AACGGCCTCT CTTCCCACAG 
ATCCTGGTGA GCATTGAGCA GGTCCAGGAA TTGTTGCCAA AAATCGAGCG GAGTGCCTCT 
GAGCCCTCTC TACACCGTGC TGTACATGCC GAGGACCTGA ATCCGCTTCT CCTTCACACC 
ACGCGCTTCC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171875.1
CDS	708..2522
Translation	MSNTSSCSSSGETSPDDTPRGGGTIRVYLPNKQRTVVNVRPGQTVYDSLDKALKVRGLSQDCCAVFRLLEGRKRLTEWDTDITPLVGEELLVEVLDDVPLTMHNFVRKTFFKLAYCDFCHKFLFNGFRCQTCGYKFHQHCSSKVPTVCVDMDTMTKRCVNNTEDCPAILIYPATNSISQSDLPLTSDSPGGELPSSPSNFCLFIRGEDGQSLQRHRSTSTPNVHMVSTLEPGAAGLLEEPFKISTVGYESSPKPSTSPPHLGSPGRKPPKSPSEPKERKQSSSDDKKKVHRGGYRDSSYYWEVHPREVSMLKRIGAGSFGTVFKGKWHGDVAIKILKVTEPTPEQLQAFKNEMQVLRKTRHVNILLFMGFMTRPNFAIITQWCEGSSLYRHLHVTETKFDTMRRIDVARQTAQGMDYLHAKNIIHRDLKSNNIFLHEGWTVKIGDFGLATVKSRWSGSQQVEQPSGSILWMAPEVIRMQDTNPYTFQSDVYGYGVVLYELMSGTLPYSNINNRDQIIFMVGRGYLSPDLSKLYSNCPKSMKRLIIDCLKFKRDERPLFPQILVSIEQVQELLPKIERSASEPSLHRAVHAEDLNPLLLHTTRFL

Target ORF information:

RefSeq Version	XM_005171818.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio A-Raf proto-oncogene, serine/threonine kinase (araf), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171818.3

ORF Insert Sequence:

ATGTCCAACA CTTCCTCCTG TTCCTCCTCG GGGGAAACCA GCCCGGACGA TACACCCCGA 
GGTGGAGGCA CCATCCGCGT CTACCTCCCT AACAAGCAGC GGACGGTGGT AAACGTTCGG 
CCTGGGCAAA CTGTGTATGA TAGTTTAGAT AAAGCCCTAA AAGTCCGAGG CCTAAGCCAG 
GATTGCTGTG CTGTCTTTCG CCTGCTTGAA GGCCGGAAGA GACTTACAGA ATGGGACACT 
GACATCACAC CCCTCGTGGG GGAAGAGCTA CTGGTAGAAG TCCTGGATGA TGTTCCCCTT 
ACCATGCACA ACTTTGTACG TAAAACGTTC TTTAAGTTAG CATACTGTGA CTTCTGCCAC 
AAGTTCTTAT TCAACGGCTT CAGGTGTCAG ACATGTGGAT ATAAATTTCA CCAGCACTGC 
AGCAGTAAAG TCCCTACAGT ATGTGTGGAC ATGGACACAA TGACAAAACG GTGTGTTAAT 
AATACAGAAG ACTGCCCTGC GATATTGATA TATCCGGCCA CCAATTCCAT ATCGCAGAGC 
GACCTACCTT TAACCTCAGA TTCTCCTGGA GGGGAGCTGC CGTCTTCCCC GTCAAACTTC 
TGTTTGTTCA TTCGAGGAGA GGACGGTCAG TCTCTCCAGA GGCATCGATC AACCTCAACA 
CCCAATGTTC ACATGGTCAG CACGCTGGAG CCTGGCGCTG CTGGCCTTTT GGAGGAGCCA 
TTCAAAATCA GCACAGTGGG GTACGAGTCC TCTCCCAAAC CTTCCACTAG CCCACCCCAC 
CTAGGGTCGC CAGGAAGAAA ACCCCCCAAA TCCCCTTCTG AACCCAAGGA ACGAAAGCAG 
TCCTCGTCTG ATGATAAAAA GAAAGTGCAC CGGGGAGGTT ACAGAGACTC CAGTTACTAC 
TGGGAGGTGC ACCCGCGGGA GGTGTCCATG TTAAAGAGGA TTGGAGCAGG CTCTTTTGGG 
ACCGTCTTTA AAGGCAAGTG GCATGGAGAC GTGGCCATCA AAATTCTTAA AGTGACAGAG 
CCCACACCGG AGCAGCTCCA GGCCTTTAAA AATGAGATGC AGGTTTTACG AAAAACACGC 
CATGTAAACA TTCTGCTGTT TATGGGCTTC ATGACAAGGC CTAATTTTGC CATTATCACA 
CAGTGGTGTG AAGGCAGCAG TCTGTATCGC CATCTGCATG TCACTGAGAC CAAGTTTGAC 
ACCATGCGGC GCATTGATGT TGCTCGCCAA ACAGCACAGG GCATGGATTA TCTTCACGCC 
AAGAATATCA TTCATCGGGA CTTGAAATCT AATAATATCT TCCTGCATGA AGGCTGGACA 
GTAAAAATCG GTGACTTTGG CTTGGCTACA GTAAAGTCTC GCTGGAGTGG CTCACAGCAG 
GTCGAACAGC CCAGTGGCTC CATCCTGTGG ATGGCTCCTG AGGTGATCAG AATGCAAGAC 
ACTAACCCGT ACACCTTCCA GTCTGATGTG TATGGATATG GAGTGGTGCT CTACGAACTG 
ATGTCTGGGA CTTTGCCTTA TTCGAATATC AACAACCGCG ATCAGATTAT TTTCATGGTG 
GGTCGTGGTT ACTTGTCTCC AGACCTCAGT AAATTATACA GTAACTGCCC GAAATCAATG 
AAGAGGCTCA TCATTGACTG TCTGAAATTC AAACGGGATG AACGGCCTCT CTTCCCACAG 
ATCCTGGTGA GCATTGAGCA GGTCCAGGAA TTGTTGCCAA AAATCGAGCG GAGTGCCTCT 
GAGCCCTCTC TACACCGTGC TGTACATGCC GAGGACCTGA ATCCGCTTCT CCTTCACACC 
ACGCGCTTCC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

araf ( XM_005171818.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171875.1 Danio rerio A-Raf proto-oncogene, serine/threonine kinase (araf), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa10445
Clone ID Related Accession (Same CDS sequence)	XM_005171818.3 , XM_005171818.4
Accession Version	XM_005171818.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase A-Raf isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005171818.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACA CTTCCTCCTG TTCCTCCTCG GGGGAAACCA GCCCGGACGA TACACCCCGA 
GGTGGAGGCA CCATCCGCGT CTACCTCCCT AACAAGCAGC GGACGGTGGT AAACGTTCGG 
CCTGGGCAAA CTGTGTATGA TAGTTTAGAT AAAGCCCTAA AAGTCCGAGG CCTAAGCCAG 
GATTGCTGTG CTGTCTTTCG CCTGCTTGAA GGCCGGAAGA GACTTACAGA ATGGGACACT 
GACATCACAC CCCTCGTGGG GGAAGAGCTA CTGGTAGAAG TCCTGGATGA TGTTCCCCTT 
ACCATGCACA ACTTTGTACG TAAAACGTTC TTTAAGTTAG CATACTGTGA CTTCTGCCAC 
AAGTTCTTAT TCAACGGCTT CAGGTGTCAG ACATGTGGAT ATAAATTTCA CCAGCACTGC 
AGCAGTAAAG TCCCTACAGT ATGTGTGGAC ATGGACACAA TGACAAAACG GTGTGTTAAT 
AATACAGAAG ACTGCCCTGC GATATTGATA TATCCGGCCA CCAATTCCAT ATCGCAGAGC 
GACCTACCTT TAACCTCAGA TTCTCCTGGA GGGGAGCTGC CGTCTTCCCC GTCAAACTTC 
TGTTTGTTCA TTCGAGGAGA GGACGGTCAG TCTCTCCAGA GGCATCGATC AACCTCAACA 
CCCAATGTTC ACATGGTCAG CACGCTGGAG CCTGGCGCTG CTGGCCTTTT GGAGGAGCCA 
TTCAAAATCA GCACAGTGGG GTACGAGTCC TCTCCCAAAC CTTCCACTAG CCCACCCCAC 
CTAGGGTCGC CAGGAAGAAA ACCCCCCAAA TCCCCTTCTG AACCCAAGGA ACGAAAGCAG 
TCCTCGTCTG ATGATAAAAA GAAAGTGCAC CGGGGAGGTT ACAGAGACTC CAGTTACTAC 
TGGGAGGTGC ACCCGCGGGA GGTGTCCATG TTAAAGAGGA TTGGAGCAGG CTCTTTTGGG 
ACCGTCTTTA AAGGCAAGTG GCATGGAGAC GTGGCCATCA AAATTCTTAA AGTGACAGAG 
CCCACACCGG AGCAGCTCCA GGCCTTTAAA AATGAGATGC AGGTTTTACG AAAAACACGC 
CATGTAAACA TTCTGCTGTT TATGGGCTTC ATGACAAGGC CTAATTTTGC CATTATCACA 
CAGTGGTGTG AAGGCAGCAG TCTGTATCGC CATCTGCATG TCACTGAGAC CAAGTTTGAC 
ACCATGCGGC GCATTGATGT TGCTCGCCAA ACAGCACAGG GCATGGATTA TCTTCACGCC 
AAGAATATCA TTCATCGGGA CTTGAAATCT AATAATATCT TCCTGCATGA AGGCTGGACA 
GTAAAAATCG GTGACTTTGG CTTGGCTACA GTAAAGTCTC GCTGGAGTGG CTCACAGCAG 
GTCGAACAGC CCAGTGGCTC CATCCTGTGG ATGGCTCCTG AGGTGATCAG AATGCAAGAC 
ACTAACCCGT ACACCTTCCA GTCTGATGTG TATGGATATG GAGTGGTGCT CTACGAACTG 
ATGTCTGGGA CTTTGCCTTA TTCGAATATC AACAACCGCG ATCAGATTAT TTTCATGGTG 
GGTCGTGGTT ACTTGTCTCC AGACCTCAGT AAATTATACA GTAACTGCCC GAAATCAATG 
AAGAGGCTCA TCATTGACTG TCTGAAATTC AAACGGGATG AACGGCCTCT CTTCCCACAG 
ATCCTGGTGA GCATTGAGCA GGTCCAGGAA TTGTTGCCAA AAATCGAGCG GAGTGCCTCT 
GAGCCCTCTC TACACCGTGC TGTACATGCC GAGGACCTGA ATCCGCTTCT CCTTCACACC 
ACGCGCTTCC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171875.1
CDS	179..1993
Translation	MSNTSSCSSSGETSPDDTPRGGGTIRVYLPNKQRTVVNVRPGQTVYDSLDKALKVRGLSQDCCAVFRLLEGRKRLTEWDTDITPLVGEELLVEVLDDVPLTMHNFVRKTFFKLAYCDFCHKFLFNGFRCQTCGYKFHQHCSSKVPTVCVDMDTMTKRCVNNTEDCPAILIYPATNSISQSDLPLTSDSPGGELPSSPSNFCLFIRGEDGQSLQRHRSTSTPNVHMVSTLEPGAAGLLEEPFKISTVGYESSPKPSTSPPHLGSPGRKPPKSPSEPKERKQSSSDDKKKVHRGGYRDSSYYWEVHPREVSMLKRIGAGSFGTVFKGKWHGDVAIKILKVTEPTPEQLQAFKNEMQVLRKTRHVNILLFMGFMTRPNFAIITQWCEGSSLYRHLHVTETKFDTMRRIDVARQTAQGMDYLHAKNIIHRDLKSNNIFLHEGWTVKIGDFGLATVKSRWSGSQQVEQPSGSILWMAPEVIRMQDTNPYTFQSDVYGYGVVLYELMSGTLPYSNINNRDQIIFMVGRGYLSPDLSKLYSNCPKSMKRLIIDCLKFKRDERPLFPQILVSIEQVQELLPKIERSASEPSLHRAVHAEDLNPLLLHTTRFL

Target ORF information:

RefSeq Version	XM_005171818.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio A-Raf proto-oncogene, serine/threonine kinase (araf), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171818.4

ORF Insert Sequence:

ATGTCCAACA CTTCCTCCTG TTCCTCCTCG GGGGAAACCA GCCCGGACGA TACACCCCGA 
GGTGGAGGCA CCATCCGCGT CTACCTCCCT AACAAGCAGC GGACGGTGGT AAACGTTCGG 
CCTGGGCAAA CTGTGTATGA TAGTTTAGAT AAAGCCCTAA AAGTCCGAGG CCTAAGCCAG 
GATTGCTGTG CTGTCTTTCG CCTGCTTGAA GGCCGGAAGA GACTTACAGA ATGGGACACT 
GACATCACAC CCCTCGTGGG GGAAGAGCTA CTGGTAGAAG TCCTGGATGA TGTTCCCCTT 
ACCATGCACA ACTTTGTACG TAAAACGTTC TTTAAGTTAG CATACTGTGA CTTCTGCCAC 
AAGTTCTTAT TCAACGGCTT CAGGTGTCAG ACATGTGGAT ATAAATTTCA CCAGCACTGC 
AGCAGTAAAG TCCCTACAGT ATGTGTGGAC ATGGACACAA TGACAAAACG GTGTGTTAAT 
AATACAGAAG ACTGCCCTGC GATATTGATA TATCCGGCCA CCAATTCCAT ATCGCAGAGC 
GACCTACCTT TAACCTCAGA TTCTCCTGGA GGGGAGCTGC CGTCTTCCCC GTCAAACTTC 
TGTTTGTTCA TTCGAGGAGA GGACGGTCAG TCTCTCCAGA GGCATCGATC AACCTCAACA 
CCCAATGTTC ACATGGTCAG CACGCTGGAG CCTGGCGCTG CTGGCCTTTT GGAGGAGCCA 
TTCAAAATCA GCACAGTGGG GTACGAGTCC TCTCCCAAAC CTTCCACTAG CCCACCCCAC 
CTAGGGTCGC CAGGAAGAAA ACCCCCCAAA TCCCCTTCTG AACCCAAGGA ACGAAAGCAG 
TCCTCGTCTG ATGATAAAAA GAAAGTGCAC CGGGGAGGTT ACAGAGACTC CAGTTACTAC 
TGGGAGGTGC ACCCGCGGGA GGTGTCCATG TTAAAGAGGA TTGGAGCAGG CTCTTTTGGG 
ACCGTCTTTA AAGGCAAGTG GCATGGAGAC GTGGCCATCA AAATTCTTAA AGTGACAGAG 
CCCACACCGG AGCAGCTCCA GGCCTTTAAA AATGAGATGC AGGTTTTACG AAAAACACGC 
CATGTAAACA TTCTGCTGTT TATGGGCTTC ATGACAAGGC CTAATTTTGC CATTATCACA 
CAGTGGTGTG AAGGCAGCAG TCTGTATCGC CATCTGCATG TCACTGAGAC CAAGTTTGAC 
ACCATGCGGC GCATTGATGT TGCTCGCCAA ACAGCACAGG GCATGGATTA TCTTCACGCC 
AAGAATATCA TTCATCGGGA CTTGAAATCT AATAATATCT TCCTGCATGA AGGCTGGACA 
GTAAAAATCG GTGACTTTGG CTTGGCTACA GTAAAGTCTC GCTGGAGTGG CTCACAGCAG 
GTCGAACAGC CCAGTGGCTC CATCCTGTGG ATGGCTCCTG AGGTGATCAG AATGCAAGAC 
ACTAACCCGT ACACCTTCCA GTCTGATGTG TATGGATATG GAGTGGTGCT CTACGAACTG 
ATGTCTGGGA CTTTGCCTTA TTCGAATATC AACAACCGCG ATCAGATTAT TTTCATGGTG 
GGTCGTGGTT ACTTGTCTCC AGACCTCAGT AAATTATACA GTAACTGCCC GAAATCAATG 
AAGAGGCTCA TCATTGACTG TCTGAAATTC AAACGGGATG AACGGCCTCT CTTCCCACAG 
ATCCTGGTGA GCATTGAGCA GGTCCAGGAA TTGTTGCCAA AAATCGAGCG GAGTGCCTCT 
GAGCCCTCTC TACACCGTGC TGTACATGCC GAGGACCTGA ATCCGCTTCT CCTTCACACC 
ACGCGCTTCC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

The Kinase Activity-deficient Isoform of the Protein Araf Antagonizes Ras/Mitogen-activated Protein Kinase (Ras/MAPK) Signaling in the Zebrafish Embryo.
The Journal of biological chemistry290(42)25512-21(2015 Oct)
Xiong C,Liu X,Meng A

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Araf kinase antagonizes Nodal-Smad2 activity in mesendoderm development by directly phosphorylating the Smad2 linker region.
Nature communications41728(2013)
Liu X,Xiong C,Jia S,Zhang Y,Chen YG,Wang Q,Meng A

Temporal pattern of loss/persistence of duplicate genes involved in signal transduction and metabolic pathways after teleost-specific genome duplication.
BMC evolutionary biology9127(2009 Jun)
Sato Y,Hashiguchi Y,Nishida M

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araf cDNA ORF clone, Danio rerio(zebrafish)

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