nek2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	nek2
Entrez Gene ID	394025
Full Name	NIMA-related kinase 2
Synonyms	zgc:55602
General protein information	Preferred Names NIMA-related kinase 2 Names serine/threonine-protein kinase Nek2 NIMA (never in mitosis gene a)-related kinase 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_201050.1	NP_957344.1	serine/threonine-protein kinase Nek2

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	fin1	NP_593305.1
Homo sapiens (human)	NEK2	NP_002488.1
Macaca mulatta (Rhesus monkey)	NEK2	XP_001108833.1
Bos taurus (cattle)	NEK2	NP_001039735.1
Gallus gallus (chicken)	NEK2	NP_001026221.1
Mus musculus (house mouse)	Nek2	NP_035022.2
Xenopus tropicalis (tropical clawed frog)	nek2	NP_001164656.1
Pan troglodytes (chimpanzee)	NEK2	XP_514178.3
Canis lupus familiaris (dog)	NEK2	XP_537144.3
Danio rerio (zebrafish)	nek2	NP_957344.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

On the crucial cerebellar wound healing-related pathways and their cross-talks after traumatic brain injury in Danio rerio.
Wu CC, Tsai TH, Chang C, Lee TT, Lin C, Cheng IH, Sun MC, Chuang YJ, Chen BS
PloS one9(6)e97902(2014)

Whole genome sequencing in patients with retinitis pigmentosa reveals pathogenic DNA structural changes and NEK2 as a new disease gene.
Nishiguchi KM, Tearle RG, Liu YP, Oh EC, Miyake N, Benaglio P, Harper S, Koskiniemi-Kuendig H, Venturini G, Sharon D, Koenekoop RK, Nakamura M, Kondo M, Ueno S, Yasuma TR, Beckmann JS, Ikegawa S, Matsumoto N, Terasaki H, Berson EL, Katsanis N, Rivolta C
Proceedings of the National Academy of Sciences of the United States of America110(40)16139-44(2013 Oct)

Characterization and development of novel small-molecules inhibiting GSK3 and activating Wnt signaling.
Zhong H, Zou H, Semenov MV, Moshinsky D, He X, Huang H, Li S, Quan J, Yang Z, Lin S
Molecular bioSystems5(11)1356-60(2009 Nov)

The zebrafish gene map defines ancestral vertebrate chromosomes.
Woods IG, Wilson C, Friedlander B, Chang P, Reyes DK, Nix R, Kelly PD, Chu F, Postlethwait JH, Talbot WS
Genome research15(9)1307-14(2005 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following nek2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nek2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa02176	NM_201050.1 Latest version!	Danio rerio NIMA-related kinase 2 (nek2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nek2 ( NM_201050.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_957344.1 Danio rerio NIMA-related kinase 2 (nek2), mRNA.

Price & Availability↑

CloneID	ODa02176
Clone ID Related Accession (Same CDS sequence)	NM_201050.1
Accession Version	NM_201050.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1542902400000
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase Nek2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC048055.1. ##Evidence-Data-START## Transcript exon combination :: BC048055.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGCCATCCA AAACTGAAGA CTACGAGGTG CTGCTCACCA TAGGATGTGG ATCTTATGGA 
AAATGCCAGA AAATCAAGAG GAAATCCGAT GGAAAGATTC TGGTCTGGAA GGTTCTGGAC 
TATGGCACTA TGGCCGAGGG AGAGAAACAG ATGCTGGTGT CAGAAGTCAA CTTGCTCCGT 
GAGCTGAAGC ACCCAAATAT CGTCCGATAC CATGACCGAA TTATTGACAG AACGAACACG 
ACATTATATA TAGTGATGGA ATACTGTGAG GGTGGAGATC TCGCCAGCCT CATCAACAGA 
AGCATCAAAG ACAAGCGATA CCTGGAGGAA GAATTCATCC TCCGTGTGAT GGCACAGTTG 
TCTCTGGCGT TAAAAGAATG CCATGGTAGG AGTAACGGCA GCAGTACAGT TCTGCACCGA 
GACCTGAAAC CAGCAAACAT CTTTCTGGAT GCCAAACAGA ATGTAAAGCT TGGCGATTTC 
GGTTTAGCTC GCATACTAAA CCACGATACA AGCTTTGCTA AAACGTTTGT TGGAACGCCA 
TATTACATGT CGCCAGAACA AATGAATCGC ATGTCCTATA ATGAGAAATC GGATATATGG 
TCTTTAGGGT GTTTACTCTA TGAACTATGT GCTTTATCGC CCCCATTTAC AGCATACAAC 
CAGACAGAGC TGGCTCGAAA AATCAGAGAA GGCAGATTTC GAAGAATCCC ATACCGATAC 
TCGGATGAGC TAAACACACT GCTTTCAAAA ATGCTCAACT TAAAGGATTA TCTGAGGCCC 
TCTGTGGAGT CCATCCTGCA GAATGGTTTG ATCTCCGGTT ATGTGGCCCT CGAGCAGAAG 
AGGCTCCAGG AGAAACAGCG GCGCAGATCA GATGAGGCAG AGCAGCCCAA ACATCCAGAG 
TCACCACTTC TGGCAGAGCT GCGGCTTAAA GAGCAGATTC TCCGAGAGCG AGAGCAGGCC 
CTCAAAGAGC GAGAGCAGCG GCTAGAGCAA AGGGAACAAG AACTGTGTGT CCGAGAACAG 
CAAACTAATG AAAAGCTGGT CAGAGCCGAG AGCATGTTGA AGGCGTTTAA TCTGATTCGA 
CAGCAGAGGG CGCTATCTCT GCTCAGCGCC AGCGACACAG AGAATGAAGA GAACATCTCT 
CCAGGGAAAA AGAGGGTTCA CTTTGCAGGA GACGGGAAGG AGAACGGCAG ACTGATCATG 
AAACCTCAGG AGCACATCCT TGAGAAGAGA CACCAGCTGA TGAACAAGCG CATACAGACA 
CTCGGAGAGG AGGAGAAGAT GATCCACTCG CCAAAACACA GAGAAATGCA GGGAATCCGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_957344.1
CDS	137..1459
Translation	MPSKTEDYEVLLTIGCGSYGKCQKIKRKSDGKILVWKVLDYGTMAEGEKQMLVSEVNLLRELKHPNIVRYHDRIIDRTNTTLYIVMEYCEGGDLASLINRSIKDKRYLEEEFILRVMAQLSLALKECHGRSNGSSTVLHRDLKPANIFLDAKQNVKLGDFGLARILNHDTSFAKTFVGTPYYMSPEQMNRMSYNEKSDIWSLGCLLYELCALSPPFTAYNQTELARKIREGRFRRIPYRYSDELNTLLSKMLNLKDYLRPSVESILQNGLISGYVALEQKRLQEKQRRRSDEAEQPKHPESPLLAELRLKEQILREREQALKEREQRLEQREQELCVREQQTNEKLVRAESMLKAFNLIRQQRALSLLSASDTENEENISPGKKRVHFAGDGKENGRLIMKPQEHILEKRHQLMNKRIQTLGEEEKMIHSPKHREMQGIR

Target ORF information:

RefSeq Version	NM_201050.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NIMA-related kinase 2 (nek2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201050.1

ORF Insert Sequence:

ATGCCATCCA AAACTGAAGA CTACGAGGTG CTGCTCACCA TAGGATGTGG ATCTTATGGA 
AAATGCCAGA AAATCAAGAG GAAATCCGAT GGAAAGATTC TGGTCTGGAA GGTTCTGGAC 
TATGGCACTA TGGCCGAGGG AGAGAAACAG ATGCTGGTGT CAGAAGTCAA CTTGCTCCGT 
GAGCTGAAGC ACCCAAATAT CGTCCGATAC CATGACCGAA TTATTGACAG AACGAACACG 
ACATTATATA TAGTGATGGA ATACTGTGAG GGTGGAGATC TCGCCAGCCT CATCAACAGA 
AGCATCAAAG ACAAGCGATA CCTGGAGGAA GAATTCATCC TCCGTGTGAT GGCACAGTTG 
TCTCTGGCGT TAAAAGAATG CCATGGTAGG AGTAACGGCA GCAGTACAGT TCTGCACCGA 
GACCTGAAAC CAGCAAACAT CTTTCTGGAT GCCAAACAGA ATGTAAAGCT TGGCGATTTC 
GGTTTAGCTC GCATACTAAA CCACGATACA AGCTTTGCTA AAACGTTTGT TGGAACGCCA 
TATTACATGT CGCCAGAACA AATGAATCGC ATGTCCTATA ATGAGAAATC GGATATATGG 
TCTTTAGGGT GTTTACTCTA TGAACTATGT GCTTTATCGC CCCCATTTAC AGCATACAAC 
CAGACAGAGC TGGCTCGAAA AATCAGAGAA GGCAGATTTC GAAGAATCCC ATACCGATAC 
TCGGATGAGC TAAACACACT GCTTTCAAAA ATGCTCAACT TAAAGGATTA TCTGAGGCCC 
TCTGTGGAGT CCATCCTGCA GAATGGTTTG ATCTCCGGTT ATGTGGCCCT CGAGCAGAAG 
AGGCTCCAGG AGAAACAGCG GCGCAGATCA GATGAGGCAG AGCAGCCCAA ACATCCAGAG 
TCACCACTTC TGGCAGAGCT GCGGCTTAAA GAGCAGATTC TCCGAGAGCG AGAGCAGGCC 
CTCAAAGAGC GAGAGCAGCG GCTAGAGCAA AGGGAACAAG AACTGTGTGT CCGAGAACAG 
CAAACTAATG AAAAGCTGGT CAGAGCCGAG AGCATGTTGA AGGCGTTTAA TCTGATTCGA 
CAGCAGAGGG CGCTATCTCT GCTCAGCGCC AGCGACACAG AGAATGAAGA GAACATCTCT 
CCAGGGAAAA AGAGGGTTCA CTTTGCAGGA GACGGGAAGG AGAACGGCAG ACTGATCATG 
AAACCTCAGG AGCACATCCT TGAGAAGAGA CACCAGCTGA TGAACAAGCG CATACAGACA 
CTCGGAGAGG AGGAGAAGAT GATCCACTCG CCAAAACACA GAGAAATGCA GGGAATCCGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

On the crucial cerebellar wound healing-related pathways and their cross-talks after traumatic brain injury in Danio rerio.
PloS one9(6)e97902(2014)
Wu CC,Tsai TH,Chang C,Lee TT,Lin C,Cheng IH,Sun MC,Chuang YJ,Chen BS

Whole genome sequencing in patients with retinitis pigmentosa reveals pathogenic DNA structural changes and NEK2 as a new disease gene.
Proceedings of the National Academy of Sciences of the United States of America110(40)16139-44(2013 Oct)
Nishiguchi KM,Tearle RG,Liu YP,Oh EC,Miyake N,Benaglio P,Harper S,Koskiniemi-Kuendig H,Venturini G,Sharon D,Koenekoop RK,Nakamura M,Kondo M,Ueno S,Yasuma TR,Beckmann JS,Ikegawa S,Matsumoto N,Terasaki H,Berson EL,Katsanis N,Rivolta C

Characterization and development of novel small-molecules inhibiting GSK3 and activating Wnt signaling.
Molecular bioSystems5(11)1356-60(2009 Nov)
Zhong H,Zou H,Semenov MV,Moshinsky D,He X,Huang H,Li S,Quan J,Yang Z,Lin S

The zebrafish gene map defines ancestral vertebrate chromosomes.
Genome research15(9)1307-14(2005 Sep)
Woods IG,Wilson C,Friedlander B,Chang P,Reyes DK,Nix R,Kelly PD,Chu F,Postlethwait JH,Talbot WS

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nek2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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