PRKG1A cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRKG1A
Entrez Gene ID	394005
Full Name	protein kinase, cGMP-dependent, type Ia
Synonyms	prkg1,zgc:63955
General protein information	Preferred Names protein kinase, cGMP-dependent, type Ia Names cGMP-dependent protein kinase 1 protein kinase, cGMP-dependent, type I
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_201030.1	NP_957324.1	cGMP-dependent protein kinase 1
XM_005157058.4	XP_005157115.1	cGMP-dependent protein kinase 1 isoform X1
XM_005157058.3	XP_005157115.1	cGMP-dependent protein kinase 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PRKG1	NP_006249.1
Mus musculus (house mouse)	Prkg1	NP_001013855.1
Gallus gallus (chicken)	PRKG1	XP_003641507.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008863	XP_319605.4
Macaca mulatta (Rhesus monkey)	PRKG1	XP_001099460.1
Rattus norvegicus (Norway rat)	Prkg1	NP_001099201.3
Canis lupus familiaris (dog)	PRKG1	XP_851997.2
Bos taurus (cattle)	PRKG1	NP_776861.1
Danio rerio (zebrafish)	prkg1a	NP_957324.1
Drosophila melanogaster (fruit fly)	for	NP_477488.1
Xenopus tropicalis (tropical clawed frog)	LOC100494278	XP_002935474.2
Pan troglodytes (chimpanzee)	PRKG1	XP_001162858.1

Gene Ontology
Bibliography

Related articles in PubMed

The role of PKG in oocyte maturation of zebrafish.
Li J, Wang Y, Zhou W, Li X, Chen H
Biochemical and biophysical research communications505(2)530-535(2018 10)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Integrative view of α2,3-sialyltransferases (ST3Gal) molecular and functional evolution in deuterostomes: significance of lineage-specific losses.
Petit D, Teppa E, Mir AM, Vicogne D, Thisse C, Thisse B, Filloux C, Harduin-Lepers A
Molecular biology and evolution32(4)906-27(2015 Apr)

Temporal pattern of loss/persistence of duplicate genes involved in signal transduction and metabolic pathways after teleost-specific genome duplication.
Sato Y, Hashiguchi Y, Nishida M
BMC evolutionary biology9127(2009 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PRKG1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRKG1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa32115	NM_201030.1 Latest version!	Danio rerio protein kinase cGMP-dependent 1a (prkg1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa19052	XM_005157058.4 Latest version!	Danio rerio protein kinase, cGMP-dependent, type Ia (prkg1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
ODa19052	XM_005157058.3	Danio rerio protein kinase, cGMP-dependent, type Ia (prkg1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRKG1A ( NM_201030.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_957324.1 Danio rerio protein kinase cGMP-dependent 1a (prkg1a), mRNA.

Price & Availability↑

CloneID	ODa32115
Clone ID Related Accession (Same CDS sequence)	NM_201030.1
Accession Version	NM_201030.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549036800000
Organism	Danio rerio(zebrafish)
Product	cGMP-dependent protein kinase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC054581.1. ##Evidence-Data-START## Transcript exon combination :: BC054581.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA898400 [ECO:0000348] ##Evidence-Data-END##

ATGAGTGACC TAGACGAAGA CTTCGCGAAG ATTTTAATGC TGAAGGAGGA GAGGATACGA 
GATCTTGAGA GGCGTTTGCT GGAGCGAGAA GACGAAATCA GCGAGCTGAA GAGAAAGCTG 
CACAAATGTC AGTCCGTTTT ACCGAGCGCG CAGATCGGCC CGCGGACACA CCGCGCGCAG 
GGAATATCAG CAGAACCGCA AACGCACCAG GATTTATCAA ACCAAAGCTT TAGACGAGTT 
GCCAAATCGG ACAGGTCAAA GGATCTGATC AAGTCAGCTA TCCTGGACAA TGACTTCATG 
AAGAATCTGG AAATGTCTCA AATCCAGGAG ATCGTGGACT GTATGTATCC AGTGGACTAT 
GACAAAAACA GCTGTATTAT TAAAGAAGGC GATGTGGGCT CCCTCGTCTA CGTCATGGAA 
GATGGTAAGG TTGAAGTGAC TAAAGAAGGA CTGAAGCTGT GCACCATGGG ACCAGGAAAA 
GTGTTTGGAG AGCTTGCCAT CCTTTATAAC TGCACCAGAA CGGCCACTGT GAGAACGGTG 
TCCAGTGTGA AGTTATGGGC CATCGACAGA CAGTGTTTCC AGACCATCAT GATGAGGACG 
GGCCTAATCA AACATGCAGA GTACATGGAG TTATTGAAGA GTGTGCTGAC GTTCCGGGGT 
CTTCCAGAGG AAATATTGAG CAAACTGGCT GATGTCCTGG AGGAGACCCA CTATGAGGAC 
GGCAACTACA TCATTCGCCA GGGGGCAAGA GGAGACACTT TCTTCATTAT CAGCAAAGGA 
AAGGTGACTA TGACGAGAGA GGACTGTCCC GGTCAGGAGC CTGTTTACCT GCGGTCAATG 
GGGAGAGGAG ATTCATTTGG GGAAAAAGCT TTGCAAGGGG AGGACATCAG AACGGCAAAC 
GTCATCGCAG CAGAGACCGT CACCTGTCTC GTGATAGACA GAGACTCTTA CAAACATCTG 
ATCGGAGGTC TGGAAGATGT TTCCAACAAA GGCTGTGAAG ATGCTGAAGC AAAAGCAAAA 
TACGAGGCAG AAAATGCCTT TTTCTCCAAT CTGAACCTGT CTGACTTTAA CATCATCGAC 
ACTCTTGGAG TTGGAGGATT TGGACGTGTG GAACTGGTTC AGCTGAAGAG TGATGAAATG 
AAGACGTTTG CGATGAAGAT CCTGAAGAAG CGTCACATTG TGGACACGAG ACAGCAGGAG 
CACATCCGCT CCGAGAAGCT CATCATGCAG GAGGCACATT CAGATTTTAT AGTCAGACTC 
TACCGCACAT TTAAGGACAG TAAATACCTC TATATGCTGA TGGAGGCCTG TTTAGGTGGA 
GAACTATGGA CAATCCTGAG AGACCGGGGA AACTTTGACG ATTCAACAAC GCGATTTTAC 
ACAGCGTGTG TGGTGGAGGC CTTCGCTTAT CTGCATTCCA AAGGCATCAT ATACAGAGAC 
CTCAAACCAG AGAACCTGAT ACTGGACCAC AGAGGATACG CCAAGCTGGT GGATTTTGGC 
TTTGCTAAAA AGATCGGCTT TGGGAAGAAG ACATGGACAT TCTGTGGTAC TCCAGAGTAT 
GTGGCTCCAG AGATCATCCT GAATAAAGGA CACGACATCT CTGCGGATTA CTGGTCTCTC 
GGTATCCTCA TGTATGAGCT GTTGACCGGC AGTCCTCCTT TTTCAGGACC AGATCCAATG 
AAAACATACA ACATCATCCT TAGAGGCATC GATATGATTG AATTCCCCAA GAAAATAACC 
AAAAATGCTG CCAATCTCAT CAAAAAGCTA TGCAGAGACA CTCCTTCTGA AAGATTAGGA 
AACTTGAAAA ACGGTGTCAA AGACATCCAA AAGCACAAAT GGTTTGAAGG GTTTAACTGG 
GACGGACTGA GGAAGGGGAC GCTGATGCCC CCCATCATCC CTAATGTAAC CTCATCCACA 
GACACCAGTA ACTTCGACAG CTTTCCAGAG GACAACGAAG ACCCACCGCC TGATGATAAC 
TCAGGCTGGG ATATAGACTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_957324.1
CDS	362..2365
Translation	MSDLDEDFAKILMLKEERIRDLERRLLEREDEISELKRKLHKCQSVLPSAQIGPRTHRAQGISAEPQTHQDLSNQSFRRVAKSDRSKDLIKSAILDNDFMKNLEMSQIQEIVDCMYPVDYDKNSCIIKEGDVGSLVYVMEDGKVEVTKEGLKLCTMGPGKVFGELAILYNCTRTATVRTVSSVKLWAIDRQCFQTIMMRTGLIKHAEYMELLKSVLTFRGLPEEILSKLADVLEETHYEDGNYIIRQGARGDTFFIISKGKVTMTREDCPGQEPVYLRSMGRGDSFGEKALQGEDIRTANVIAAETVTCLVIDRDSYKHLIGGLEDVSNKGCEDAEAKAKYEAENAFFSNLNLSDFNIIDTLGVGGFGRVELVQLKSDEMKTFAMKILKKRHIVDTRQQEHIRSEKLIMQEAHSDFIVRLYRTFKDSKYLYMLMEACLGGELWTILRDRGNFDDSTTRFYTACVVEAFAYLHSKGIIYRDLKPENLILDHRGYAKLVDFGFAKKIGFGKKTWTFCGTPEYVAPEIILNKGHDISADYWSLGILMYELLTGSPPFSGPDPMKTYNIILRGIDMIEFPKKITKNAANLIKKLCRDTPSERLGNLKNGVKDIQKHKWFEGFNWDGLRKGTLMPPIIPNVTSSTDTSNFDSFPEDNEDPPPDDNSGWDIDF

Target ORF information:

RefSeq Version	NM_201030.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein kinase cGMP-dependent 1a (prkg1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201030.1

ORF Insert Sequence:

ATGAGTGACC TAGACGAAGA CTTCGCGAAG ATTTTAATGC TGAAGGAGGA GAGGATACGA 
GATCTTGAGA GGCGTTTGCT GGAGCGAGAA GACGAAATCA GCGAGCTGAA GAGAAAGCTG 
CACAAATGTC AGTCCGTTTT ACCGAGCGCG CAGATCGGCC CGCGGACACA CCGCGCGCAG 
GGAATATCAG CAGAACCGCA AACGCACCAG GATTTATCAA ACCAAAGCTT TAGACGAGTT 
GCCAAATCGG ACAGGTCAAA GGATCTGATC AAGTCAGCTA TCCTGGACAA TGACTTCATG 
AAGAATCTGG AAATGTCTCA AATCCAGGAG ATCGTGGACT GTATGTATCC AGTGGACTAT 
GACAAAAACA GCTGTATTAT TAAAGAAGGC GATGTGGGCT CCCTCGTCTA CGTCATGGAA 
GATGGTAAGG TTGAAGTGAC TAAAGAAGGA CTGAAGCTGT GCACCATGGG ACCAGGAAAA 
GTGTTTGGAG AGCTTGCCAT CCTTTATAAC TGCACCAGAA CGGCCACTGT GAGAACGGTG 
TCCAGTGTGA AGTTATGGGC CATCGACAGA CAGTGTTTCC AGACCATCAT GATGAGGACG 
GGCCTAATCA AACATGCAGA GTACATGGAG TTATTGAAGA GTGTGCTGAC GTTCCGGGGT 
CTTCCAGAGG AAATATTGAG CAAACTGGCT GATGTCCTGG AGGAGACCCA CTATGAGGAC 
GGCAACTACA TCATTCGCCA GGGGGCAAGA GGAGACACTT TCTTCATTAT CAGCAAAGGA 
AAGGTGACTA TGACGAGAGA GGACTGTCCC GGTCAGGAGC CTGTTTACCT GCGGTCAATG 
GGGAGAGGAG ATTCATTTGG GGAAAAAGCT TTGCAAGGGG AGGACATCAG AACGGCAAAC 
GTCATCGCAG CAGAGACCGT CACCTGTCTC GTGATAGACA GAGACTCTTA CAAACATCTG 
ATCGGAGGTC TGGAAGATGT TTCCAACAAA GGCTGTGAAG ATGCTGAAGC AAAAGCAAAA 
TACGAGGCAG AAAATGCCTT TTTCTCCAAT CTGAACCTGT CTGACTTTAA CATCATCGAC 
ACTCTTGGAG TTGGAGGATT TGGACGTGTG GAACTGGTTC AGCTGAAGAG TGATGAAATG 
AAGACGTTTG CGATGAAGAT CCTGAAGAAG CGTCACATTG TGGACACGAG ACAGCAGGAG 
CACATCCGCT CCGAGAAGCT CATCATGCAG GAGGCACATT CAGATTTTAT AGTCAGACTC 
TACCGCACAT TTAAGGACAG TAAATACCTC TATATGCTGA TGGAGGCCTG TTTAGGTGGA 
GAACTATGGA CAATCCTGAG AGACCGGGGA AACTTTGACG ATTCAACAAC GCGATTTTAC 
ACAGCGTGTG TGGTGGAGGC CTTCGCTTAT CTGCATTCCA AAGGCATCAT ATACAGAGAC 
CTCAAACCAG AGAACCTGAT ACTGGACCAC AGAGGATACG CCAAGCTGGT GGATTTTGGC 
TTTGCTAAAA AGATCGGCTT TGGGAAGAAG ACATGGACAT TCTGTGGTAC TCCAGAGTAT 
GTGGCTCCAG AGATCATCCT GAATAAAGGA CACGACATCT CTGCGGATTA CTGGTCTCTC 
GGTATCCTCA TGTATGAGCT GTTGACCGGC AGTCCTCCTT TTTCAGGACC AGATCCAATG 
AAAACATACA ACATCATCCT TAGAGGCATC GATATGATTG AATTCCCCAA GAAAATAACC 
AAAAATGCTG CCAATCTCAT CAAAAAGCTA TGCAGAGACA CTCCTTCTGA AAGATTAGGA 
AACTTGAAAA ACGGTGTCAA AGACATCCAA AAGCACAAAT GGTTTGAAGG GTTTAACTGG 
GACGGACTGA GGAAGGGGAC GCTGATGCCC CCCATCATCC CTAATGTAAC CTCATCCACA 
GACACCAGTA ACTTCGACAG CTTTCCAGAG GACAACGAAG ACCCACCGCC TGATGATAAC 
TCAGGCTGGG ATATAGACTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKG1A ( XM_005157058.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005157115.1 Danio rerio protein kinase, cGMP-dependent, type Ia (prkg1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa19052
Clone ID Related Accession (Same CDS sequence)	XM_005157058.4 , XM_005157058.3
Accession Version	XM_005157058.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	cGMP-dependent protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005157058.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGG GGACTTTACG CGACCTCCAG TACGCGCTTC AGGAGAAAAT CGAAGAACTC 
AGGCAGAGAG ACGCGCTCAT TGACGAACTG GAGCTCGAAC TGGACCAGAA GGATGAACTC 
ATTCAGAAGC TGCAGAACGA GCTGGACAAG TACCGATCCG TGATCCGTCC GGCGACCCAA 
CAGGTCCAGA CGCGCTCGGA GCTGCCGGAG CATCAGCGCA CCAAACGGCA GGCGATCAGC 
GCCGAACCCG CAAACATCCA GCAGCTGAGT CACGTGACGC TGCCCAACGT CCCCAAAAGC 
GCCAAGTCAA AGGATCTGAT CAAGTCAGCT ATCCTGGACA ATGACTTCAT GAAGAATCTG 
GAAATGTCTC AAATCCAGGA GATCGTGGAC TGTATGTATC CAGTGGACTA TGACAAAAAC 
AGCTGTATTA TTAAAGAAGG CGATGTGGGC TCCCTCGTCT ACGTCATGGA AGATGGTAAG 
GTTGAAGTGA CTAAAGAAGG ACTGAAGCTG TGCACCATGG GACCAGGAAA AGTGTTTGGA 
GAGCTTGCCA TCCTTTATAA CTGCACCAGA ACGGCCACTG TGAGAACGGT GTCCAGTGTG 
AAGTTATGGG CCATCGACAG ACAGTGTTTC CAGACCATCA TGATGAGGAC GGGCCTAATC 
AAACATGCAG AGTACATGGA GTTATTGAAG AGTGTGCTGA CGTTCCGGGG TCTTCCAGAG 
GAAATATTGA GCAAACTGGC TGATGTCCTG GAGGAGACCC ACTATGAGGA CGGCAACTAC 
ATCATTCGCC AGGGGGCAAG AGGAGACACT TTCTTCATTA TCAGCAAAGG AAAGGTGACG 
ATGACGAGAG AGGACTGTCC CGGTCAGGAG CCTGTTTACC TGCGGTCAAT GGGGAGAGGA 
GATTCATTTG GGGAAAAAGC TTTGCAAGGG GAGGACATCA GAACGGCAAA CGTCATCGCA 
GCAGAGACCG TCACCTGTCT TGTGATAGAC AGAGACTCTT ACAAACATCT GATCGGAGGT 
CTGGAAGATG TTTCCAACAA AGGCTGTGAA GATGCTGAAG CAAAAGCAAA ATACGAGGCA 
GAAAATGCCT TTTTCTCCAA TCTGAACCTG TCTGACTTTA ACATCATCGA CACTCTTGGA 
GTTGGAGGAT TTGGACGTGT GGAACTGGTT CAGCTGAAGA GTGATGAAAT GAAGACGTTT 
GCGATGAAGA TCCTGAAGAA GCGTCACATT GTGGACACGA GACAGCAGGA GCACATCCGC 
TCCGAGAAGC TCATCATGCA GGAGGCACAT TCAGATTTTA TAGTCAGACT CTACCGCACA 
TTTAAGGACA GTAAATACCT CTATATGCTG ATGGAGGCCT GTTTAGGTGG AGAACTATGG 
ACAATCCTGA GAGACCGGGG AAACTTTGAC GATTCAACAA CGCGATTTTA CACAGCGTGT 
GTGGTGGAGG CCTTCGCTTA TCTACATTCC AAAGGCATCA TATACAGAGA CCTCAAACCA 
GAGAACCTGA TACTGGACCA CAGAGGATAC GCCAAGCTGG TGGATTTTGG CTTTGCTAAA 
AAGATCGGCT TTGGGAAGAA GACATGGACA TTCTGTGGTA CTCCAGAGTA TGTGGCTCCA 
GAGATCATCC TGAATAAAGG ACACGACATC TCTGCGGATT ACTGGTCTCT CGGTATCCTC 
ATGTATGAGC TGTTGACCGG CAGTCCTCCT TTTTCAGGAC CAGATCCAAT GAAAACATAC 
AACATCATCC TTAGAGGCAT CGATATGATT GAATTCCCCA AGAAAATAAC CAAAAATGCT 
GCCAATCTCA TCAAAAAGCT ATGCAGAGAC ACTCCTTCTG AAAGATTAGG AAACTTGAAA 
AACGGTGTCA AAGACATCCA AAAGCACAAA TGGTTTGAAG GGTTTAACTG GGACGGACTG 
AGGAAGGGGA CGCTGATGCC CCCCATCATC CCTAATGTAA CCTCATCCAC AGACACCAGT 
AACTTCGACA GCTTTCCAGA GGACAACGAA GACCCACCGC CTGATGATAA CTCAGGCTGG 
GATATAGACT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157115.1
CDS	345..2399
Translation	MMMGTLRDLQYALQEKIEELRQRDALIDELELELDQKDELIQKLQNELDKYRSVIRPATQQVQTRSELPEHQRTKRQAISAEPANIQQLSHVTLPNVPKSAKSKDLIKSAILDNDFMKNLEMSQIQEIVDCMYPVDYDKNSCIIKEGDVGSLVYVMEDGKVEVTKEGLKLCTMGPGKVFGELAILYNCTRTATVRTVSSVKLWAIDRQCFQTIMMRTGLIKHAEYMELLKSVLTFRGLPEEILSKLADVLEETHYEDGNYIIRQGARGDTFFIISKGKVTMTREDCPGQEPVYLRSMGRGDSFGEKALQGEDIRTANVIAAETVTCLVIDRDSYKHLIGGLEDVSNKGCEDAEAKAKYEAENAFFSNLNLSDFNIIDTLGVGGFGRVELVQLKSDEMKTFAMKILKKRHIVDTRQQEHIRSEKLIMQEAHSDFIVRLYRTFKDSKYLYMLMEACLGGELWTILRDRGNFDDSTTRFYTACVVEAFAYLHSKGIIYRDLKPENLILDHRGYAKLVDFGFAKKIGFGKKTWTFCGTPEYVAPEIILNKGHDISADYWSLGILMYELLTGSPPFSGPDPMKTYNIILRGIDMIEFPKKITKNAANLIKKLCRDTPSERLGNLKNGVKDIQKHKWFEGFNWDGLRKGTLMPPIIPNVTSSTDTSNFDSFPEDNEDPPPDDNSGWDIDF

Target ORF information:

RefSeq Version	XM_005157058.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein kinase, cGMP-dependent, type Ia (prkg1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157058.4

ORF Insert Sequence:

ATGATGATGG GGACTTTACG CGACCTCCAG TACGCGCTTC AGGAGAAAAT CGAAGAACTC 
AGGCAGAGAG ACGCGCTCAT TGACGAACTG GAGCTCGAAC TGGACCAGAA GGATGAACTC 
ATTCAGAAGC TGCAGAACGA GCTGGACAAG TACCGATCCG TGATCCGTCC GGCGACCCAA 
CAGGTCCAGA CGCGCTCGGA GCTGCCGGAG CATCAGCGCA CCAAACGGCA GGCGATCAGC 
GCCGAACCCG CAAACATCCA GCAGCTGAGT CACGTGACGC TGCCCAACGT CCCCAAAAGC 
GCCAAGTCAA AGGATCTGAT CAAGTCAGCT ATCCTGGACA ATGACTTCAT GAAGAATCTG 
GAAATGTCTC AAATCCAGGA GATCGTGGAC TGTATGTATC CAGTGGACTA TGACAAAAAC 
AGCTGTATTA TTAAAGAAGG CGATGTGGGC TCCCTCGTCT ACGTCATGGA AGATGGTAAG 
GTTGAAGTGA CTAAAGAAGG ACTGAAGCTG TGCACCATGG GACCAGGAAA AGTGTTTGGA 
GAGCTTGCCA TCCTTTATAA CTGCACCAGA ACGGCCACTG TGAGAACGGT GTCCAGTGTG 
AAGTTATGGG CCATCGACAG ACAGTGTTTC CAGACCATCA TGATGAGGAC GGGCCTAATC 
AAACATGCAG AGTACATGGA GTTATTGAAG AGTGTGCTGA CGTTCCGGGG TCTTCCAGAG 
GAAATATTGA GCAAACTGGC TGATGTCCTG GAGGAGACCC ACTATGAGGA CGGCAACTAC 
ATCATTCGCC AGGGGGCAAG AGGAGACACT TTCTTCATTA TCAGCAAAGG AAAGGTGACG 
ATGACGAGAG AGGACTGTCC CGGTCAGGAG CCTGTTTACC TGCGGTCAAT GGGGAGAGGA 
GATTCATTTG GGGAAAAAGC TTTGCAAGGG GAGGACATCA GAACGGCAAA CGTCATCGCA 
GCAGAGACCG TCACCTGTCT TGTGATAGAC AGAGACTCTT ACAAACATCT GATCGGAGGT 
CTGGAAGATG TTTCCAACAA AGGCTGTGAA GATGCTGAAG CAAAAGCAAA ATACGAGGCA 
GAAAATGCCT TTTTCTCCAA TCTGAACCTG TCTGACTTTA ACATCATCGA CACTCTTGGA 
GTTGGAGGAT TTGGACGTGT GGAACTGGTT CAGCTGAAGA GTGATGAAAT GAAGACGTTT 
GCGATGAAGA TCCTGAAGAA GCGTCACATT GTGGACACGA GACAGCAGGA GCACATCCGC 
TCCGAGAAGC TCATCATGCA GGAGGCACAT TCAGATTTTA TAGTCAGACT CTACCGCACA 
TTTAAGGACA GTAAATACCT CTATATGCTG ATGGAGGCCT GTTTAGGTGG AGAACTATGG 
ACAATCCTGA GAGACCGGGG AAACTTTGAC GATTCAACAA CGCGATTTTA CACAGCGTGT 
GTGGTGGAGG CCTTCGCTTA TCTACATTCC AAAGGCATCA TATACAGAGA CCTCAAACCA 
GAGAACCTGA TACTGGACCA CAGAGGATAC GCCAAGCTGG TGGATTTTGG CTTTGCTAAA 
AAGATCGGCT TTGGGAAGAA GACATGGACA TTCTGTGGTA CTCCAGAGTA TGTGGCTCCA 
GAGATCATCC TGAATAAAGG ACACGACATC TCTGCGGATT ACTGGTCTCT CGGTATCCTC 
ATGTATGAGC TGTTGACCGG CAGTCCTCCT TTTTCAGGAC CAGATCCAAT GAAAACATAC 
AACATCATCC TTAGAGGCAT CGATATGATT GAATTCCCCA AGAAAATAAC CAAAAATGCT 
GCCAATCTCA TCAAAAAGCT ATGCAGAGAC ACTCCTTCTG AAAGATTAGG AAACTTGAAA 
AACGGTGTCA AAGACATCCA AAAGCACAAA TGGTTTGAAG GGTTTAACTG GGACGGACTG 
AGGAAGGGGA CGCTGATGCC CCCCATCATC CCTAATGTAA CCTCATCCAC AGACACCAGT 
AACTTCGACA GCTTTCCAGA GGACAACGAA GACCCACCGC CTGATGATAA CTCAGGCTGG 
GATATAGACT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKG1A ( XM_005157058.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005157115.1 Danio rerio protein kinase, cGMP-dependent, type Ia (prkg1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa19052
Clone ID Related Accession (Same CDS sequence)	XM_005157058.4 , XM_005157058.3
Accession Version	XM_005157058.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	cGMP-dependent protein kinase 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005157058.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGG GGACTTTACG CGACCTCCAG TACGCGCTTC AGGAGAAAAT CGAAGAACTC 
AGGCAGAGAG ACGCGCTCAT TGACGAACTG GAGCTCGAAC TGGACCAGAA GGATGAACTC 
ATTCAGAAGC TGCAGAACGA GCTGGACAAG TACCGATCCG TGATCCGTCC GGCGACCCAA 
CAGGTCCAGA CGCGCTCGGA GCTGCCGGAG CATCAGCGCA CCAAACGGCA GGCGATCAGC 
GCCGAACCCG CAAACATCCA GCAGCTGAGT CACGTGACGC TGCCCAACGT CCCCAAAAGC 
GCCAAGTCAA AGGATCTGAT CAAGTCAGCT ATCCTGGACA ATGACTTCAT GAAGAATCTG 
GAAATGTCTC AAATCCAGGA GATCGTGGAC TGTATGTATC CAGTGGACTA TGACAAAAAC 
AGCTGTATTA TTAAAGAAGG CGATGTGGGC TCCCTCGTCT ACGTCATGGA AGATGGTAAG 
GTTGAAGTGA CTAAAGAAGG ACTGAAGCTG TGCACCATGG GACCAGGAAA AGTGTTTGGA 
GAGCTTGCCA TCCTTTATAA CTGCACCAGA ACGGCCACTG TGAGAACGGT GTCCAGTGTG 
AAGTTATGGG CCATCGACAG ACAGTGTTTC CAGACCATCA TGATGAGGAC GGGCCTAATC 
AAACATGCAG AGTACATGGA GTTATTGAAG AGTGTGCTGA CGTTCCGGGG TCTTCCAGAG 
GAAATATTGA GCAAACTGGC TGATGTCCTG GAGGAGACCC ACTATGAGGA CGGCAACTAC 
ATCATTCGCC AGGGGGCAAG AGGAGACACT TTCTTCATTA TCAGCAAAGG AAAGGTGACG 
ATGACGAGAG AGGACTGTCC CGGTCAGGAG CCTGTTTACC TGCGGTCAAT GGGGAGAGGA 
GATTCATTTG GGGAAAAAGC TTTGCAAGGG GAGGACATCA GAACGGCAAA CGTCATCGCA 
GCAGAGACCG TCACCTGTCT TGTGATAGAC AGAGACTCTT ACAAACATCT GATCGGAGGT 
CTGGAAGATG TTTCCAACAA AGGCTGTGAA GATGCTGAAG CAAAAGCAAA ATACGAGGCA 
GAAAATGCCT TTTTCTCCAA TCTGAACCTG TCTGACTTTA ACATCATCGA CACTCTTGGA 
GTTGGAGGAT TTGGACGTGT GGAACTGGTT CAGCTGAAGA GTGATGAAAT GAAGACGTTT 
GCGATGAAGA TCCTGAAGAA GCGTCACATT GTGGACACGA GACAGCAGGA GCACATCCGC 
TCCGAGAAGC TCATCATGCA GGAGGCACAT TCAGATTTTA TAGTCAGACT CTACCGCACA 
TTTAAGGACA GTAAATACCT CTATATGCTG ATGGAGGCCT GTTTAGGTGG AGAACTATGG 
ACAATCCTGA GAGACCGGGG AAACTTTGAC GATTCAACAA CGCGATTTTA CACAGCGTGT 
GTGGTGGAGG CCTTCGCTTA TCTACATTCC AAAGGCATCA TATACAGAGA CCTCAAACCA 
GAGAACCTGA TACTGGACCA CAGAGGATAC GCCAAGCTGG TGGATTTTGG CTTTGCTAAA 
AAGATCGGCT TTGGGAAGAA GACATGGACA TTCTGTGGTA CTCCAGAGTA TGTGGCTCCA 
GAGATCATCC TGAATAAAGG ACACGACATC TCTGCGGATT ACTGGTCTCT CGGTATCCTC 
ATGTATGAGC TGTTGACCGG CAGTCCTCCT TTTTCAGGAC CAGATCCAAT GAAAACATAC 
AACATCATCC TTAGAGGCAT CGATATGATT GAATTCCCCA AGAAAATAAC CAAAAATGCT 
GCCAATCTCA TCAAAAAGCT ATGCAGAGAC ACTCCTTCTG AAAGATTAGG AAACTTGAAA 
AACGGTGTCA AAGACATCCA AAAGCACAAA TGGTTTGAAG GGTTTAACTG GGACGGACTG 
AGGAAGGGGA CGCTGATGCC CCCCATCATC CCTAATGTAA CCTCATCCAC AGACACCAGT 
AACTTCGACA GCTTTCCAGA GGACAACGAA GACCCACCGC CTGATGATAA CTCAGGCTGG 
GATATAGACT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157115.1
CDS	240..2294
Translation	MMMGTLRDLQYALQEKIEELRQRDALIDELELELDQKDELIQKLQNELDKYRSVIRPATQQVQTRSELPEHQRTKRQAISAEPANIQQLSHVTLPNVPKSAKSKDLIKSAILDNDFMKNLEMSQIQEIVDCMYPVDYDKNSCIIKEGDVGSLVYVMEDGKVEVTKEGLKLCTMGPGKVFGELAILYNCTRTATVRTVSSVKLWAIDRQCFQTIMMRTGLIKHAEYMELLKSVLTFRGLPEEILSKLADVLEETHYEDGNYIIRQGARGDTFFIISKGKVTMTREDCPGQEPVYLRSMGRGDSFGEKALQGEDIRTANVIAAETVTCLVIDRDSYKHLIGGLEDVSNKGCEDAEAKAKYEAENAFFSNLNLSDFNIIDTLGVGGFGRVELVQLKSDEMKTFAMKILKKRHIVDTRQQEHIRSEKLIMQEAHSDFIVRLYRTFKDSKYLYMLMEACLGGELWTILRDRGNFDDSTTRFYTACVVEAFAYLHSKGIIYRDLKPENLILDHRGYAKLVDFGFAKKIGFGKKTWTFCGTPEYVAPEIILNKGHDISADYWSLGILMYELLTGSPPFSGPDPMKTYNIILRGIDMIEFPKKITKNAANLIKKLCRDTPSERLGNLKNGVKDIQKHKWFEGFNWDGLRKGTLMPPIIPNVTSSTDTSNFDSFPEDNEDPPPDDNSGWDIDF

Target ORF information:

RefSeq Version	XM_005157058.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio protein kinase, cGMP-dependent, type Ia (prkg1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157058.3

ORF Insert Sequence:

ATGATGATGG GGACTTTACG CGACCTCCAG TACGCGCTTC AGGAGAAAAT CGAAGAACTC 
AGGCAGAGAG ACGCGCTCAT TGACGAACTG GAGCTCGAAC TGGACCAGAA GGATGAACTC 
ATTCAGAAGC TGCAGAACGA GCTGGACAAG TACCGATCCG TGATCCGTCC GGCGACCCAA 
CAGGTCCAGA CGCGCTCGGA GCTGCCGGAG CATCAGCGCA CCAAACGGCA GGCGATCAGC 
GCCGAACCCG CAAACATCCA GCAGCTGAGT CACGTGACGC TGCCCAACGT CCCCAAAAGC 
GCCAAGTCAA AGGATCTGAT CAAGTCAGCT ATCCTGGACA ATGACTTCAT GAAGAATCTG 
GAAATGTCTC AAATCCAGGA GATCGTGGAC TGTATGTATC CAGTGGACTA TGACAAAAAC 
AGCTGTATTA TTAAAGAAGG CGATGTGGGC TCCCTCGTCT ACGTCATGGA AGATGGTAAG 
GTTGAAGTGA CTAAAGAAGG ACTGAAGCTG TGCACCATGG GACCAGGAAA AGTGTTTGGA 
GAGCTTGCCA TCCTTTATAA CTGCACCAGA ACGGCCACTG TGAGAACGGT GTCCAGTGTG 
AAGTTATGGG CCATCGACAG ACAGTGTTTC CAGACCATCA TGATGAGGAC GGGCCTAATC 
AAACATGCAG AGTACATGGA GTTATTGAAG AGTGTGCTGA CGTTCCGGGG TCTTCCAGAG 
GAAATATTGA GCAAACTGGC TGATGTCCTG GAGGAGACCC ACTATGAGGA CGGCAACTAC 
ATCATTCGCC AGGGGGCAAG AGGAGACACT TTCTTCATTA TCAGCAAAGG AAAGGTGACG 
ATGACGAGAG AGGACTGTCC CGGTCAGGAG CCTGTTTACC TGCGGTCAAT GGGGAGAGGA 
GATTCATTTG GGGAAAAAGC TTTGCAAGGG GAGGACATCA GAACGGCAAA CGTCATCGCA 
GCAGAGACCG TCACCTGTCT TGTGATAGAC AGAGACTCTT ACAAACATCT GATCGGAGGT 
CTGGAAGATG TTTCCAACAA AGGCTGTGAA GATGCTGAAG CAAAAGCAAA ATACGAGGCA 
GAAAATGCCT TTTTCTCCAA TCTGAACCTG TCTGACTTTA ACATCATCGA CACTCTTGGA 
GTTGGAGGAT TTGGACGTGT GGAACTGGTT CAGCTGAAGA GTGATGAAAT GAAGACGTTT 
GCGATGAAGA TCCTGAAGAA GCGTCACATT GTGGACACGA GACAGCAGGA GCACATCCGC 
TCCGAGAAGC TCATCATGCA GGAGGCACAT TCAGATTTTA TAGTCAGACT CTACCGCACA 
TTTAAGGACA GTAAATACCT CTATATGCTG ATGGAGGCCT GTTTAGGTGG AGAACTATGG 
ACAATCCTGA GAGACCGGGG AAACTTTGAC GATTCAACAA CGCGATTTTA CACAGCGTGT 
GTGGTGGAGG CCTTCGCTTA TCTACATTCC AAAGGCATCA TATACAGAGA CCTCAAACCA 
GAGAACCTGA TACTGGACCA CAGAGGATAC GCCAAGCTGG TGGATTTTGG CTTTGCTAAA 
AAGATCGGCT TTGGGAAGAA GACATGGACA TTCTGTGGTA CTCCAGAGTA TGTGGCTCCA 
GAGATCATCC TGAATAAAGG ACACGACATC TCTGCGGATT ACTGGTCTCT CGGTATCCTC 
ATGTATGAGC TGTTGACCGG CAGTCCTCCT TTTTCAGGAC CAGATCCAAT GAAAACATAC 
AACATCATCC TTAGAGGCAT CGATATGATT GAATTCCCCA AGAAAATAAC CAAAAATGCT 
GCCAATCTCA TCAAAAAGCT ATGCAGAGAC ACTCCTTCTG AAAGATTAGG AAACTTGAAA 
AACGGTGTCA AAGACATCCA AAAGCACAAA TGGTTTGAAG GGTTTAACTG GGACGGACTG 
AGGAAGGGGA CGCTGATGCC CCCCATCATC CCTAATGTAA CCTCATCCAC AGACACCAGT 
AACTTCGACA GCTTTCCAGA GGACAACGAA GACCCACCGC CTGATGATAA CTCAGGCTGG 
GATATAGACT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The role of PKG in oocyte maturation of zebrafish.
Biochemical and biophysical research communications505(2)530-535(2018 10)
Li J,Wang Y,Zhou W,Li X,Chen H

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Integrative view of α2,3-sialyltransferases (ST3Gal) molecular and functional evolution in deuterostomes: significance of lineage-specific losses.
Molecular biology and evolution32(4)906-27(2015 Apr)
Petit D,Teppa E,Mir AM,Vicogne D,Thisse C,Thisse B,Filloux C,Harduin-Lepers A

Temporal pattern of loss/persistence of duplicate genes involved in signal transduction and metabolic pathways after teleost-specific genome duplication.
BMC evolutionary biology9127(2009 Jun)
Sato Y,Hashiguchi Y,Nishida M

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PRKG1A cDNA ORF clone, Danio rerio(Zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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