mgat1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mgat1a
Entrez Gene ID	393649
Full Name	mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase a
Synonyms	zgc:66030
General protein information	Preferred Names mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase a Names alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_200676.1	NP_956970.1	alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
XM_005157757.3	XP_005157814.1	alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MGAT1	NP_001015653.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005347	XP_315359.3
Canis lupus familiaris (dog)	MGAT1	XP_855658.1
Mus musculus (house mouse)	Mgat1	NP_001103620.1
Danio rerio (zebrafish)	mgat1a	NP_956970.1
Drosophila melanogaster (fruit fly)	Mgat1	NP_525117.2
Caenorhabditis elegans (roundworm)	gly-13	NP_509566.1
Xenopus tropicalis (tropical clawed frog)	mgat1	NP_001011350.1
Oryza sativa (rice)	Os02g0832800	NP_001048631.1
Pan troglodytes (chimpanzee)	MGAT1	XP_001155433.2
Danio rerio (zebrafish)	mgat1b	NP_001073440.1
Homo sapiens (human)	MGAT1	NP_002397.2
Rattus norvegicus (Norway rat)	Mgat1	NP_110488.1
Macaca mulatta (Rhesus monkey)	MGAT1	XP_001103617.1
Caenorhabditis elegans (roundworm)	gly-14	NP_497719.1
Arabidopsis thaliana (thale cress)	CGL1	NP_195537.2

Gene Ontology
Bibliography

Related articles in PubMed

Integration of Tmc1/2 into the mechanotransduction complex in zebrafish hair cells is regulated by Transmembrane O-methyltransferase (Tomt).
Erickson T, Morgan CP, Olt J, Hardy K, Busch-Nentwich E, Maeda R, Clemens R, Krey JF, Nechiporuk A, Barr-Gillespie PG, Marcotti W, Nicolson T
eLife6(2017 05)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

The zebrafish maternal-effect gene mission impossible encodes the DEAH-box helicase Dhx16 and is essential for the expression of downstream endodermal genes.
Putiri E, Pelegri F
Developmental biology353(2)275-89(2011 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mgat1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mgat1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa35356	NM_200676.1 Latest version!	Danio rerio alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase a (mgat1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ODa20715	XM_005157757.3 Latest version!	Danio rerio mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase a (mgat1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mgat1a ( NM_200676.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956970.1 Danio rerio alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase a (mgat1a), mRNA.

Price & Availability↑

CloneID	ODa35356
Clone ID Related Accession (Same CDS sequence)	NM_200676.1
Accession Version	NM_200676.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1567872000000
Organism	Danio rerio(zebrafish)
Product	alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC058297.1. ##Evidence-Data-START## Transcript exon combination :: BC058297.1, CA473494.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGCTCCGCA AGAGAAGTCC TCTTGTCATT TGTGGTGCTT TCATATTCGT AGCATGGAAT 
GTTGTTCTCC TTTTTGTCCT CATGAGGCGC CCTTCAAGTC CAGGGACTTT CAATAACCAG 
GATAAACCTG GAGAGACTGA GCACAGAGCC GAAGGAGGCA AGTTTGGAAA TATCATGAAT 
GAAGTGATTC GAGTAGCCGA TGCATTCGAA GCTGAACTTG CAGCTCAAAA GAAGATCCTA 
CAGCAGATCC AGAGCCACTG GTCAGTGTGG GACAGCAAAG ATGGCGTGAT TCCTGAGAAG 
TCGAAATCAG AAGTTGAACA CACAGCTCCG GTGGTCATCC CGATTTTAGT CATTGCCTGT 
AATCGCGTGA CGGTGAAACG CTGCCTGGAT AAACTCATCG AGCACCGTCC ATCAGCCGAG 
CTTCATCCCA TCATAGTGAG CCAGGACTGC GGACACAGAG AGACGTCTGA CGTGATTGGC 
TCATACGGCA GTCAGCTGAC ACACATAAAA CAGCCGGATC TATCGGATGT CGCCGTGCCT 
CCTCAGCATA AGAAGTTTCA GGGTTACTAC AAAATCTCTC GCCACTACAA ATGGGCCCTC 
AGCCAGGTCT TCAACACCTT TTCGTACTCT TCGGTTGTTG TTGTGGAGGA CGATTTGGAG 
GTGGCCCCTG ATTTCTTCGA GTACTTCAGA GCGCTTCATC CTATGTTAAA ATCCGACCCG 
ACGCTGTGGT GCGTGTCTGC CTGGAATGAC AACGGCCGGG ATGGATTCGT AGACCCTGGA 
AAAGCGAGCC TCCTGTACAG GACTGATTTT TTCCCAGGCC TGGGCTGGAT GCTCACAAAA 
GACCTCTGGG CAGAGCTTGA GCCAAAATGG CCGGCCTCCT TTTGGGATGA CTGGATGCGC 
CACCCTGATC AGCGCAAAGA CAGATCCTGT ATCAGACCGG AAATATCCAG AACGTTAACC 
TTCGGTCGAA AGGGTGTGAG TTTGGGGCAG TTCTACGACA AGTACCTCCG CTTTATTAAG 
CTCAACACCG AATTTGTGCC TTTTACAAAA ATGGACCTGT CTTATTTAGA GAAGGAGAAA 
TATGATGAGA GCTTTGAGAA GGAGGTTTAC GCTGCTTCAC TTGTCACATT GGAGGACTTA 
AAGAGTGGTA AACTGTCTGG ATCTGGCCCA TTTCGGGTGC AATATTCTAG TCCGGACAGT 
TTTAAAAGTT TGGCTCGCAA TCTCGGCGTA ATGGACGACC TGAAATCTGG AGTCCCAAGA 
GCTGGGTATA GAGGAGCAGT CAGCTTTCTT TTACGTGGGA AGAGGGTTTA TTTAGCTCCA 
CCTGCTGGTT GGAGTCGGTA TGATCCAAGC TGGAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956970.1
CDS	41..1399
Translation	MLRKRSPLVICGAFIFVAWNVVLLFVLMRRPSSPGTFNNQDKPGETEHRAEGGKFGNIMNEVIRVADAFEAELAAQKKILQQIQSHWSVWDSKDGVIPEKSKSEVEHTAPVVIPILVIACNRVTVKRCLDKLIEHRPSAELHPIIVSQDCGHRETSDVIGSYGSQLTHIKQPDLSDVAVPPQHKKFQGYYKISRHYKWALSQVFNTFSYSSVVVVEDDLEVAPDFFEYFRALHPMLKSDPTLWCVSAWNDNGRDGFVDPGKASLLYRTDFFPGLGWMLTKDLWAELEPKWPASFWDDWMRHPDQRKDRSCIRPEISRTLTFGRKGVSLGQFYDKYLRFIKLNTEFVPFTKMDLSYLEKEKYDESFEKEVYAASLVTLEDLKSGKLSGSGPFRVQYSSPDSFKSLARNLGVMDDLKSGVPRAGYRGAVSFLLRGKRVYLAPPAGWSRYDPSWS

Target ORF information:

RefSeq Version	NM_200676.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase a (mgat1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200676.1

ORF Insert Sequence:

ATGCTCCGCA AGAGAAGTCC TCTTGTCATT TGTGGTGCTT TCATATTCGT AGCATGGAAT 
GTTGTTCTCC TTTTTGTCCT CATGAGGCGC CCTTCAAGTC CAGGGACTTT CAATAACCAG 
GATAAACCTG GAGAGACTGA GCACAGAGCC GAAGGAGGCA AGTTTGGAAA TATCATGAAT 
GAAGTGATTC GAGTAGCCGA TGCATTCGAA GCTGAACTTG CAGCTCAAAA GAAGATCCTA 
CAGCAGATCC AGAGCCACTG GTCAGTGTGG GACAGCAAAG ATGGCGTGAT TCCTGAGAAG 
TCGAAATCAG AAGTTGAACA CACAGCTCCG GTGGTCATCC CGATTTTAGT CATTGCCTGT 
AATCGCGTGA CGGTGAAACG CTGCCTGGAT AAACTCATCG AGCACCGTCC ATCAGCCGAG 
CTTCATCCCA TCATAGTGAG CCAGGACTGC GGACACAGAG AGACGTCTGA CGTGATTGGC 
TCATACGGCA GTCAGCTGAC ACACATAAAA CAGCCGGATC TATCGGATGT CGCCGTGCCT 
CCTCAGCATA AGAAGTTTCA GGGTTACTAC AAAATCTCTC GCCACTACAA ATGGGCCCTC 
AGCCAGGTCT TCAACACCTT TTCGTACTCT TCGGTTGTTG TTGTGGAGGA CGATTTGGAG 
GTGGCCCCTG ATTTCTTCGA GTACTTCAGA GCGCTTCATC CTATGTTAAA ATCCGACCCG 
ACGCTGTGGT GCGTGTCTGC CTGGAATGAC AACGGCCGGG ATGGATTCGT AGACCCTGGA 
AAAGCGAGCC TCCTGTACAG GACTGATTTT TTCCCAGGCC TGGGCTGGAT GCTCACAAAA 
GACCTCTGGG CAGAGCTTGA GCCAAAATGG CCGGCCTCCT TTTGGGATGA CTGGATGCGC 
CACCCTGATC AGCGCAAAGA CAGATCCTGT ATCAGACCGG AAATATCCAG AACGTTAACC 
TTCGGTCGAA AGGGTGTGAG TTTGGGGCAG TTCTACGACA AGTACCTCCG CTTTATTAAG 
CTCAACACCG AATTTGTGCC TTTTACAAAA ATGGACCTGT CTTATTTAGA GAAGGAGAAA 
TATGATGAGA GCTTTGAGAA GGAGGTTTAC GCTGCTTCAC TTGTCACATT GGAGGACTTA 
AAGAGTGGTA AACTGTCTGG ATCTGGCCCA TTTCGGGTGC AATATTCTAG TCCGGACAGT 
TTTAAAAGTT TGGCTCGCAA TCTCGGCGTA ATGGACGACC TGAAATCTGG AGTCCCAAGA 
GCTGGGTATA GAGGAGCAGT CAGCTTTCTT TTACGTGGGA AGAGGGTTTA TTTAGCTCCA 
CCTGCTGGTT GGAGTCGGTA TGATCCAAGC TGGAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mgat1a ( XM_005157757.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005157814.1 Danio rerio mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase a (mgat1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa20715
Clone ID Related Accession (Same CDS sequence)	XM_005157757.3
Accession Version	XM_005157757.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1509bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005157757.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCAAA CACGTCATGC AAATGATGTG AAAACAAATT CAAATATTCA CAAGACTCTT 
CCTGCTCATC CATCCACCAA ACCCTCCTAT TCTAAATGCT CTATCCACAT CTTCCTTCCT 
GAATCCTGTG TCCCATCCTG TGTCAAGGCC ATGCTCCGCA AGAGAAGTCC TCTTGTCATT 
TGTGGTGCTT TCATATTCGT AGCATGGAAT GTTGTTCTCC TTTTTGTCCT TATGAGGCGC 
CCTTCAAGTC CCGGGACTTT CAATAACCAG GATAAACCTG GAGAGACTGA GCACAGAGCC 
GAAGGAGGCA AGTTTGGAAA TATCATGAAT GAAGTGATTC GAGTAGCCGA TGCATTCGAA 
GCTGAACTTG CAGCTCAAAA GAAGATCCTA CAGCAGATCC AGAGCCACTG GTCAGTGTGG 
GACAGCAAAG ATGGCGTGAT TCCTGAGAAG TCGAAATCAG AAGTTGAACA CACAGCTCCG 
GTGGTCATCC CGATTTTAGT CATTGCCTGT AATCGCGTGA CGGTGAAACG CTGCCTGGAT 
AAACTCATCG AGCACCGTCC ATCAGCCGAG CTTCATCCCA TCATAGTGAG CCAGGACTGC 
GGACACAGAG AGACGTCTGA CGTGATTGGC TCATACGGCA GTCAGCTGAC ACACATAAAA 
CAGCCGGATC TATCGGATGT CGCCGTGCCT CCTCAGCATA AGAAGTTTCA GGGTTACTAC 
AAAATCTCTC GCCACTACAA ATGGGCCCTC AGCCAGGTCT TCAACACCTT TTCGTACTCT 
TCGGTTGTTG TTGTGGAGGA CGATTTGGAG GTGGCCCCTG ATTTCTTCGA GTACTTCAGA 
GCGCTTCATC CTATGTTAAA ATCCGACCCG ACGCTGTGGT GCGTGTCTGC CTGGAATGAC 
AACGGCCGGG ATGGATTCGT AGACCCTGGA AAAGCGAGCC TCCTGTACAG GACTGATTTT 
TTCCCAGGCC TGGGCTGGAT GCTCACAAAA GACCTCTGGG CAGAGCTTGA GCCAAAATGG 
CCGGCCTCCT TTTGGGATGA CTGGATGCGC CACCCTGATC AGCGCAAAGA CAGATCCTGT 
ATCAGACCGG AAATATCCAG AACGTTAACC TTCGGTCGAA AGGGTGTGAG TTTGGGGCAG 
TTCTACGACA AGTACCTCCG CTTTATTAAG CTCAACACCG AATTTGTGCC TTTTACAAAA 
ATGGACCTGT CTTATTTAGA GAAGGAGAAA TATGATGAGA GCTTTGAGAA GGAGGTTTAC 
GCTGCTTCAC TTGTCACATT GGAGGACTTA AAGAGTGGTA AACTGTCTGG ATCTGGCCCA 
TTTCGGGTGC AATATTCTAG TCCGGACAGT TTTAAAAGTT TGGCTCGCAA TCTCGGCGTA 
ATGGACGACC TGAAATCTGG AGTCCCAAGA GCTGGGTATA GAGGAGCAGT CAGCTTTCTT 
TTACGTGGGA AGAGGGTTTA TTTAGCTCCA CCTGCTGGTT GGAGTCGGTA TGATCCAAGC 
TGGAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157814.1
CDS	357..1865
Translation	MPQTRHANDVKTNSNIHKTLPAHPSTKPSYSKCSIHIFLPESCVPSCVKAMLRKRSPLVICGAFIFVAWNVVLLFVLMRRPSSPGTFNNQDKPGETEHRAEGGKFGNIMNEVIRVADAFEAELAAQKKILQQIQSHWSVWDSKDGVIPEKSKSEVEHTAPVVIPILVIACNRVTVKRCLDKLIEHRPSAELHPIIVSQDCGHRETSDVIGSYGSQLTHIKQPDLSDVAVPPQHKKFQGYYKISRHYKWALSQVFNTFSYSSVVVVEDDLEVAPDFFEYFRALHPMLKSDPTLWCVSAWNDNGRDGFVDPGKASLLYRTDFFPGLGWMLTKDLWAELEPKWPASFWDDWMRHPDQRKDRSCIRPEISRTLTFGRKGVSLGQFYDKYLRFIKLNTEFVPFTKMDLSYLEKEKYDESFEKEVYAASLVTLEDLKSGKLSGSGPFRVQYSSPDSFKSLARNLGVMDDLKSGVPRAGYRGAVSFLLRGKRVYLAPPAGWSRYDPSWS

Target ORF information:

RefSeq Version	XM_005157757.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase a (mgat1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157757.3

ORF Insert Sequence:

ATGCCTCAAA CACGTCATGC AAATGATGTG AAAACAAATT CAAATATTCA CAAGACTCTT 
CCTGCTCATC CATCCACCAA ACCCTCCTAT TCTAAATGCT CTATCCACAT CTTCCTTCCT 
GAATCCTGTG TCCCATCCTG TGTCAAGGCC ATGCTCCGCA AGAGAAGTCC TCTTGTCATT 
TGTGGTGCTT TCATATTCGT AGCATGGAAT GTTGTTCTCC TTTTTGTCCT TATGAGGCGC 
CCTTCAAGTC CCGGGACTTT CAATAACCAG GATAAACCTG GAGAGACTGA GCACAGAGCC 
GAAGGAGGCA AGTTTGGAAA TATCATGAAT GAAGTGATTC GAGTAGCCGA TGCATTCGAA 
GCTGAACTTG CAGCTCAAAA GAAGATCCTA CAGCAGATCC AGAGCCACTG GTCAGTGTGG 
GACAGCAAAG ATGGCGTGAT TCCTGAGAAG TCGAAATCAG AAGTTGAACA CACAGCTCCG 
GTGGTCATCC CGATTTTAGT CATTGCCTGT AATCGCGTGA CGGTGAAACG CTGCCTGGAT 
AAACTCATCG AGCACCGTCC ATCAGCCGAG CTTCATCCCA TCATAGTGAG CCAGGACTGC 
GGACACAGAG AGACGTCTGA CGTGATTGGC TCATACGGCA GTCAGCTGAC ACACATAAAA 
CAGCCGGATC TATCGGATGT CGCCGTGCCT CCTCAGCATA AGAAGTTTCA GGGTTACTAC 
AAAATCTCTC GCCACTACAA ATGGGCCCTC AGCCAGGTCT TCAACACCTT TTCGTACTCT 
TCGGTTGTTG TTGTGGAGGA CGATTTGGAG GTGGCCCCTG ATTTCTTCGA GTACTTCAGA 
GCGCTTCATC CTATGTTAAA ATCCGACCCG ACGCTGTGGT GCGTGTCTGC CTGGAATGAC 
AACGGCCGGG ATGGATTCGT AGACCCTGGA AAAGCGAGCC TCCTGTACAG GACTGATTTT 
TTCCCAGGCC TGGGCTGGAT GCTCACAAAA GACCTCTGGG CAGAGCTTGA GCCAAAATGG 
CCGGCCTCCT TTTGGGATGA CTGGATGCGC CACCCTGATC AGCGCAAAGA CAGATCCTGT 
ATCAGACCGG AAATATCCAG AACGTTAACC TTCGGTCGAA AGGGTGTGAG TTTGGGGCAG 
TTCTACGACA AGTACCTCCG CTTTATTAAG CTCAACACCG AATTTGTGCC TTTTACAAAA 
ATGGACCTGT CTTATTTAGA GAAGGAGAAA TATGATGAGA GCTTTGAGAA GGAGGTTTAC 
GCTGCTTCAC TTGTCACATT GGAGGACTTA AAGAGTGGTA AACTGTCTGG ATCTGGCCCA 
TTTCGGGTGC AATATTCTAG TCCGGACAGT TTTAAAAGTT TGGCTCGCAA TCTCGGCGTA 
ATGGACGACC TGAAATCTGG AGTCCCAAGA GCTGGGTATA GAGGAGCAGT CAGCTTTCTT 
TTACGTGGGA AGAGGGTTTA TTTAGCTCCA CCTGCTGGTT GGAGTCGGTA TGATCCAAGC 
TGGAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Integration of Tmc1/2 into the mechanotransduction complex in zebrafish hair cells is regulated by Transmembrane O-methyltransferase (Tomt).
eLife6(2017 05)
Erickson T,Morgan CP,Olt J,Hardy K,Busch-Nentwich E,Maeda R,Clemens R,Krey JF,Nechiporuk A,Barr-Gillespie PG,Marcotti W,Nicolson T

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

The zebrafish maternal-effect gene mission impossible encodes the DEAH-box helicase Dhx16 and is essential for the expression of downstream endodermal genes.
Developmental biology353(2)275-89(2011 May)
Putiri E,Pelegri F

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mgat1a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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