tigarb cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tigarb
Entrez Gene ID	393160
Full Name	tp53-induced glycolysis and apoptosis regulator b
Synonyms	c12orf5b,si:ch211-240j22.3,zgc:56074
General protein information	Preferred Names tp53-induced glycolysis and apoptosis regulator b Names fructose-2,6-bisphosphatase TIGAR B probable fructose-2,6-bisphosphatase TIGAR B
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_200191.1	NP_956485.1	fructose-2,6-bisphosphatase TIGAR B

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	tigara	NP_001034925.1
Danio rerio (zebrafish)	tigarb	NP_956485.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Molecular evolution and functional divergence of zebrafish (Danio rerio) cryptochrome genes.
Liu C, Hu J, Qu C, Wang L, Huang G, Niu P, Zhong Z, Hong F, Wang G, Postlethwait JH, Wang H
Scientific reports58113(2015 Jan)

TigarB causes mitochondrial dysfunction and neuronal loss in PINK1 deficiency.
Flinn LJ, Keatinge M, Bretaud S, Mortiboys H, Matsui H, De Felice E, Woodroof HI, Brown L, McTighe A, Soellner R, Allen CE, Heath PR, Milo M, Muqit MM, Reichert AS, Köster RW, Ingham PW, Bandmann O
Annals of neurology74(6)837-47(2013 Dec)

Comparative genomic analysis of teleost fish bmal genes.
Wang H
Genetica136(1)149-61(2009 May)

Structural and biochemical studies of TIGAR (TP53-induced glycolysis and apoptosis regulator).
Li H, Jogl G
The Journal of biological chemistry284(3)1748-54(2009 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following tigarb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tigarb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa00808	NM_200191.1 Latest version!	Danio rerio TP53 induced glycolysis regulatory phosphatase b (tigarb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tigarb ( NM_200191.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956485.1 Danio rerio TP53 induced glycolysis regulatory phosphatase b (tigarb), mRNA.

Price & Availability↑

CloneID	ODa00808
Clone ID Related Accession (Same CDS sequence)	NM_200191.1
Accession Version	NM_200191.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1561219200000
Organism	Danio rerio(zebrafish)
Product	fructose-2,6-bisphosphatase TIGAR B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC045897.1. ##Evidence-Data-START## Transcript exon combination :: BC045897.1, EH433377.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGCTCACAT TTGCATTAAC GATCGTTCGA CATGGAGAAA CACAGTACAA CAGAGATAAA 
CTGCTGCAAG GTCAGGGTAT TGACACACCT CTGTCAGACA CGGGACATCA GCAGGCGGCG 
GCTGCTGGAC GCTACCTAAA AGATCTGCAT TTCACCAATG TCTTTGTCAG TAACCTGCAA 
AGAGCTATAC AGACAGCTGA GATCATTTTA GGAAACAATC TTCATTCTTC TGCGACTGAG 
ATGATATTGG ATCCTTTACT CAGAGAGAGG GGTTTTGGGG TTGCAGAGGG AAGACCAAAG 
GAACATTTGA AGAACATGGC GAATGCAGCA GGTCAATCCT GTAGGGATTA CACACCGCCC 
GGGGGAGAGA CATTGGAACA GGTAAAGACT CGCTTCAAGA TGTTCCTCAA ATCCTTGTTT 
CAGCGCATGT TCGAGGAGCA CGGCTCTGCG TTGAGCTCTG TGCCATCGGA AGCAGATCAG 
CCTGTGATCG CTGGACTGGC AGACGATGGA GCACAGAACG TACCCGTCCA CGCTCTGATG 
GTCAGTCACG GGGCGTTTAT CCGCATATCT GTGCGTCATC TGGTGGAGGA TCTGCAGTGC 
TGTCTGCCCG CAGGTCTTAA GATGAACCAG GTGTTTTCTC CATGTCCAAA CACTGGCATC 
AGCAGGTTCA TCTTCACCAT CCATAGGGAA GAGAGTGTCC TGCGGGCCAC ACGCATACAG 
GGGGTCTTTA TCAACAGAAA AGACCACCTG GAGGAAGTCA AAAACTCTGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956485.1
CDS	125..898
Translation	MLTFALTIVRHGETQYNRDKLLQGQGIDTPLSDTGHQQAAAAGRYLKDLHFTNVFVSNLQRAIQTAEIILGNNLHSSATEMILDPLLRERGFGVAEGRPKEHLKNMANAAGQSCRDYTPPGGETLEQVKTRFKMFLKSLFQRMFEEHGSALSSVPSEADQPVIAGLADDGAQNVPVHALMVSHGAFIRISVRHLVEDLQCCLPAGLKMNQVFSPCPNTGISRFIFTIHREESVLRATRIQGVFINRKDHLEEVKNSD

Target ORF information:

RefSeq Version	NM_200191.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio TP53 induced glycolysis regulatory phosphatase b (tigarb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200191.1

ORF Insert Sequence:

ATGCTCACAT TTGCATTAAC GATCGTTCGA CATGGAGAAA CACAGTACAA CAGAGATAAA 
CTGCTGCAAG GTCAGGGTAT TGACACACCT CTGTCAGACA CGGGACATCA GCAGGCGGCG 
GCTGCTGGAC GCTACCTAAA AGATCTGCAT TTCACCAATG TCTTTGTCAG TAACCTGCAA 
AGAGCTATAC AGACAGCTGA GATCATTTTA GGAAACAATC TTCATTCTTC TGCGACTGAG 
ATGATATTGG ATCCTTTACT CAGAGAGAGG GGTTTTGGGG TTGCAGAGGG AAGACCAAAG 
GAACATTTGA AGAACATGGC GAATGCAGCA GGTCAATCCT GTAGGGATTA CACACCGCCC 
GGGGGAGAGA CATTGGAACA GGTAAAGACT CGCTTCAAGA TGTTCCTCAA ATCCTTGTTT 
CAGCGCATGT TCGAGGAGCA CGGCTCTGCG TTGAGCTCTG TGCCATCGGA AGCAGATCAG 
CCTGTGATCG CTGGACTGGC AGACGATGGA GCACAGAACG TACCCGTCCA CGCTCTGATG 
GTCAGTCACG GGGCGTTTAT CCGCATATCT GTGCGTCATC TGGTGGAGGA TCTGCAGTGC 
TGTCTGCCCG CAGGTCTTAA GATGAACCAG GTGTTTTCTC CATGTCCAAA CACTGGCATC 
AGCAGGTTCA TCTTCACCAT CCATAGGGAA GAGAGTGTCC TGCGGGCCAC ACGCATACAG 
GGGGTCTTTA TCAACAGAAA AGACCACCTG GAGGAAGTCA AAAACTCTGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Molecular evolution and functional divergence of zebrafish (Danio rerio) cryptochrome genes.
Scientific reports58113(2015 Jan)
Liu C,Hu J,Qu C,Wang L,Huang G,Niu P,Zhong Z,Hong F,Wang G,Postlethwait JH,Wang H

TigarB causes mitochondrial dysfunction and neuronal loss in PINK1 deficiency.
Annals of neurology74(6)837-47(2013 Dec)
Flinn LJ,Keatinge M,Bretaud S,Mortiboys H,Matsui H,De Felice E,Woodroof HI,Brown L,McTighe A,Soellner R,Allen CE,Heath PR,Milo M,Muqit MM,Reichert AS,Köster RW,Ingham PW,Bandmann O

Comparative genomic analysis of teleost fish bmal genes.
Genetica136(1)149-61(2009 May)
Wang H

Structural and biochemical studies of TIGAR (TP53-induced glycolysis and apoptosis regulator).
The Journal of biological chemistry284(3)1748-54(2009 Jan)
Li H,Jogl G

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tigarb cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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