VPS13A cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	VPS13A
Entrez Gene ID	378837
Full Name	vacuolar protein sorting 13 homolog A (S. cerevisiae)
Synonyms	chac,wu:fc10c04
General protein information	Preferred Names vacuolar protein sorting 13 homolog A (S. cerevisiae) Names vacuolar protein sorting-associated protein 13A
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005167334.4	XP_005167391.1	vacuolar protein sorting-associated protein 13A isoform X1
NM_001118893.2	NP_001112365.1	vacuolar protein sorting-associated protein 13A
XM_009304310.2	XP_009302585.1	vacuolar protein sorting-associated protein 13A isoform X3
XM_009304311.1	XP_009302586.1	vacuolar protein sorting-associated protein 13A isoform X4
XM_009304309.3	XP_009302584.1	vacuolar protein sorting-associated protein 13A isoform X2
XM_009304310.3	XP_009302585.1	vacuolar protein sorting-associated protein 13A isoform X3
XM_005167334.3	XP_005167391.1	vacuolar protein sorting-associated protein 13A isoform X1
XM_009304309.2	XP_009302584.1	vacuolar protein sorting-associated protein 13A isoform X2

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_06537	XP_003717004.1
Eremothecium gossypii	AGOS_ACL064C	NP_983340.2
Homo sapiens (human)	VPS13A	NP_150648.2
Macaca mulatta (Rhesus monkey)	LOC705323	XP_002808150.1
Canis lupus familiaris (dog)	VPS13A	XP_533522.3
Drosophila melanogaster (fruit fly)	Vps13	NP_001260781.1
Saccharomyces cerevisiae (baker's yeast)	VPS13	NP_013060.1
Bos taurus (cattle)	VPS13A	XP_002689716.3
Kluyveromyces lactis	KLLA0E00705g	XP_453978.1
Rattus norvegicus (Norway rat)	Vps13a	NP_001094445.2
Pan troglodytes (chimpanzee)	VPS13A	XP_001148547.1
Mus musculus (house mouse)	Vps13a	NP_766616.2
Xenopus tropicalis (tropical clawed frog)	vps13a	XP_002937242.2
Neurospora crassa	NCU05837	XP_960097.1
Gallus gallus (chicken)	VPS13A	NP_001264765.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005082	XP_558472.3
Danio rerio (zebrafish)	vps13a	NP_001112365.1
Caenorhabditis elegans (roundworm)	T08G11.1	NP_740900.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bayés À, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 03)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

A protein disulfide isomerase expressed in the embryonic midline is required for left/right asymmetries.
Hoshijima K, Metherall JE, Grunwald DJ
Genes & development16(19)2518-29(2002 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following VPS13A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS13A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa40323	XM_009304311.1 Latest version!	Danio rerio vacuolar protein sorting 13 homolog A (S. cerevisiae) (vps13a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa34180	NM_001118893.2 Latest version!	Danio rerio vacuolar protein sorting 13 homolog A (vps13a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa14675	XM_005167334.4 Latest version!	Danio rerio vacuolar protein sorting 13 homolog A (S. cerevisiae) (vps13a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa40321	XM_009304309.3 Latest version!	Danio rerio vacuolar protein sorting 13 homolog A (S. cerevisiae) (vps13a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa40322	XM_009304310.3 Latest version!	Danio rerio vacuolar protein sorting 13 homolog A (S. cerevisiae) (vps13a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
ODa40322	XM_009304310.2	Danio rerio vacuolar protein sorting 13 homolog A (S. cerevisiae) (vps13a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa14675	XM_005167334.3	Danio rerio vacuolar protein sorting 13 homolog A (S. cerevisiae) (vps13a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa40321	XM_009304309.2	Danio rerio vacuolar protein sorting 13 homolog A (S. cerevisiae) (vps13a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VPS13A ( XM_009304311.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009302586.1 Danio rerio vacuolar protein sorting 13 homolog A (S. cerevisiae) (vps13a), transcript variant X4, mRNA.

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CloneID	ODa40323
Clone ID Related Accession (Same CDS sequence)	XM_009304311.1
Accession Version	XM_009304311.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	vacuolar protein sorting-associated protein 13A isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCACAT CCAGGATGAT GGCGATGCGT CAGGACCGAG AGGACAGTGT GATGGTGGAC 
GTCAGCTACC GCCAGAGTCG GGACGGGACT CGTCTGGACA CCCTCGTGCA AGACCTTTAT 
CTTTGTGCCA GTATGGAGTT TCTGCTCACT GTAGCTGATG TGTTTCTTAA AGCTCTGTCT 
GACGGCTTCT CCGGTCCTCC AAAGAGTAAC AAACAACCGG CCAGCACCTC TAAAGACCCA 
GCGGCTCCTG CTCCACCAGT GGTTGCTAAA ACCGAGATGA ATGTGGTGGT GAAGAACCCA 
GAGATCGTGT TTGTGGCGGA TCTGACGCGT GCTGAAGCTC CGGCTCTGGT CATGACCACT 
CAGTGTGAGC TGGTGATGAA GAGCGAGCCC AGCGGTCAGT GCATGACGGC CGCCATTTCA 
GACCTGAAGA TTGTGGCCTG TCCGTTCCTG CGGGAGAAGA GGGAGAACAA CGTGACGACG 
GTCCTACAGC CCTGCGAGGT TTTCTTCCAG AGCCGACAGT CAAACACTGA GCCGCAGTGC 
ATCGAGCTGT CCATCAGCGC ACTCACGCTC AAGGTGTCTC CGATCATCAT AAACACGGTC 
ATCACCATTC AGACGGCTCT AAGTCCTGCC GCTGAGACGC CGCAGGAGCT GGAGAGCCCC 
ATCCCACAGG ACCTATGGGA GCGCCGCAGC CTTCGCGACC TGAAGCTCTG GTTCCTGGAG 
GAGGAGGGCG AGGAGGTCAG CACGTCCGTC ACGCGTCTCA TTCCTCAGGG AGAGTCTCTG 
AAGATGAGCA TCGCCTCCAT CTGCCTGACG CTGGAGGCCG GAGTGGGACA CCGCACCGTC 
CCCATGCTGC TGGCCAAAGC CTCGTTCCAG GGGGACGTCA AGGACTGGAG CACTCTCATC 
AACCTGCACT GCCACCTCAG CATGGAGGTG AACTACTATA ATGAGATGAT GGGTTTATGG 
GAGCCGCTGT TGGAGCCGCT GGACGATGAG GATAAAGACG GTTTTAGACC CTGGACTCTC 
GCACTGAAGA TGAAGAAGAA GCCGGTGGTC AGTTCTGGGG TGTCTGATGA GGTGGACGGC 
GCTGTGCCTG ATTATAAAAC GGTCATCAGC TTCACTAGTA AAGACCAGCT TAACGTCACC 
CTGTCCAAGT GTGGTCTGCA CATGCTCAGT AATCTGGGAA CGGCTTTTGC AGAGGCCGCT 
AAACAGACGG CCGAGTGCTT TCAGACAGAT CAGACACCAT TCGTGGTGAA GAATCGCCTG 
GGTTTGGCGG TGTCTGTGCT GCACAGCGAA GTGTTTCGAC CCATCGGGAA GGAGTCTAAG 
GATCGTGTGG TGGAGCTCCC GGATGGAGAG AGTCTGAATT TGGACTATAC CCTCACCGCC 
GAGTCCAGCG ACCAGTTCTC TGCCATGACC AGCCTCAGTG CCAAAAACTA CATACAGCCC 
ACTCCAGTCG GCCACACGAC CGCAAGTGTG ATCCCGCTCA TCAAAACGGG CCGGGGCATG 
TACAGCGTCA TGCACAACGA GTCCCGCGTC ACTCGCTTCT TCGTCTGTCA GATTTACACC 
GTCGAGGGCA GCAAATATGT CAAGATTCGC TCTCCCTTCC AGATTATCAA CCACTTTTCA 
GTGCCGTTCC GTGTTTATGA AGGATCCGTG TTTCTGGGCG AGTCTCTGCC GACAGAGGAG 
TTCTGTGTGC CGCTGGGATC CTACAGAGCT GAGCTGAGTC TGCAGCCTGT GCCTGATGGC 
GGTGAAGAGT ACGAGCACTC CGAGAGCTTC ACCTACACTG AGGTTGTCCA GAAACCCGGA 
AAGTGTCTGG AGAAAAACTG CCATCTGCGT GGTGACAGCG CCCAGATTCT GAAGGTCAAC 
ATGGTTCCTC TTGCGGACAC AGTGACGTCC CACAGCAGCG AGGAGGAGTT CGATAAGCCC 
ATCGTACTGC ATTTGTGGCC CACCGTCCTG CTGCGCAACC TGCTGCCCTA CCCAATTACT 
TTCAAGCTAA AGGGCATCGG AGATTCCCCC TCGGTGTCGC TGAAGGAGGG CCACTCATCC 
CAGATCCACA CCGCCATCAT GGACTTCTCC GTGTTGGATC TGAGGCTGCT GGGATACTTG 
CAGCAGGACT GGGAGTCTCA GTACAGCCTC AGCAGTGAAC AAGACGAGAT CAGCTTCATT 
AAGTTTCGCT CGCTCGCACG TGAGGAGGAG ACTGAGAGCC GGCCGGAGCT GGACATCGCT 
ATGCATGTGA AGAAGGAGGA GGGTCAGGTT GTGCTGGCCG TACACTCGCC GTACTGGATG 
GTGAATAAAA CAAGCCGACT GCTGCAGTAC AAGGCGGATG ATATTCATCG CAAACACCCC 
AAGGATTACG ACATGCCGCT GCTCTTCTCC TTCAAGCCAC GCAACTTCCT GCAAAACAAC 
AAGATCCGCC TGATGATCTC AGAAAGTGAG CTGTCAGATG ATTTCTCCAT CGACACTGTC 
GGGAGTTACG GAGATGTCAA ATGCAAAGGC AGACAGAAGG ATTACTTGGT GGGAGTGAAG 
ATCGATGCTA GCAGCTTCAG CTTGACACGT TTGGTGACTT TTCTGCCATT CTACATGCTG 
GTGAACAGAA CCAAACATGT GATTCGGGTC TGTGAGGAGG GGCAGGAGAA CTGGACAGAA 
GCCCGACCCG GACAGGCTGC TGTTCCATTC TGGCCAGAAA GAGACTCAAA GAAGCTGAGA 
GTGAAGGTGG AGGGCAGTCA GTCGTCACCA CGCGCCTTTG ACTTCCACCA GCCAGAGAAC 
TGCCTCCTGC TGCATCTGGA CAAAAGCTTG GGTGGCATCA TTGTGGATGT GAATCTGACA 
GAGCATTCAG CTGTGATCCT CTTCTCTGAT TATCATGATG GAGCCGCTCC CTTCCTCCTC 
ATCAACCACA CTAAGGACGA GACGCTGCAG TACCACCAGA GCAACAGTAA GAGGAAGCCT 
TCGACGTGGT TTCAGGATGA AATACTGGAT TTATCAATGA GGGATGAGAC TGATTCCCTC 
TGCTCTCAGG CCGAGATCGA TGAACTGAGT CCAGGTCAGG CGGTTTACTA CACCTGGGCT 
GAACCAACCG GCTCCAGGAT CCTCAGCTGG AAACTGGGCA AATACAAAGG AGAGCTCAAG 
AGTGAAGAGG ATCAGTTGCT GGATGTGGAC AAGGAGGGCA AGTTGTTTTT GGTGTCGTTT 
TATGAAGGCC TCCAGCGTGT GGCGCTCTTC ACCAGCGAGC AGCGCATCTT CAAGAAGATC 
TGCGACAGTG AGAAGATAGA GCTGGCTCAG CAAGAAATCA GCATCTTGCT GCAGAACATG 
GGCATTTCGC TGGTCAACAA CAACTCCAGC CAGGAAGTGG CCTTCATTGG CATCACCAGC 
TCTGATGTTG TGTGGGAAAC CAAGCCGAAG AAGCGCAGCC GCTGGAAAGT TCTGAGTGTT 
AAAGAGGCAG CAGTTCTGGA GCAGCACTAC AGAGACTACA TGGAGAACGG CCCAGTGGAC 
AGCACCATCA TAGACCTGGA CAACGACTAC CAGGTGTTGT TTGCTGCTAA CGGTGTGGAC 
ATGAGGATCA TGCAGCCCTA CGATGCTCCA CTCAGAAGGA ACTTCCTGCC GGCGTTGAAG 
GTGGAGTACA GCGTTTCAGC CCGCCAGAAA ACATACAGAG TCCAGATCAA CAGGATCCAG 
ATCCAAAACC AGCTGCAAGG AGCCATATTC CCGTTTGTGT TTTACCCCAT CAAACCGCCA 
AAGTCTGTCA GCATGGACTC AGAGCCAAAA CCGCTGGCTG ACGTGAGCAT CATCACCAGA 
GCAGCAGGAC ACTCCGACAT TCTGCGCATC AAATATTTTA TGGTGTTGAT TCAGGAGATG 
GACCTGCGGC TGGATCTGGG CTTTCTGTAT GCCATACTTG AGCTCTTCAC TGCTGAACAC 
ACCAGTGCTG TGACGTCTGA ACAGGAGGTG GAGTTGTTTG ATAAAGACAT AGAGTATCTA 
AAGACTGAGT TAAATCATGT TTCAGCTACA GACACATCTC CCATCAGCCT CTACGAGTAC 
TTCCACATCT CTCCTATAAA GCTTCACTTG AGCTTGTCTC TGAGCAGTGG TGGTGAAGAC 
AGCAATAAGC AAGAGCGCGA GACGGAGCTG ATCCCAGTTC AGTCTCTCCA TCTGCTGCTC 
AAGAGCATCG GGGCTACACT TACAGACGCA CAGGACGTCC TCTTTAAACT GGGCTTCTTC 
GAGCTGAGGT ATCAGTTTCG CACCACTCAG CAGCTGCAGT GGGAGGTCGT TCGCCATTAC 
TCCAAACAGG CTATCAAGCA GATGTATGTC CTTGTTCTGG GTTTGGACGT TCTTGGGAAT 
CCATTCGGGC TGATCCGGGG CCTGTCTGAA GGCGTCGAGG CTTTCTTTTA CGAGCCCTAT 
CAGGGTGCAA TTCAGGGTCC AGAGGAGTTC ATGGAGGGCA TGGCTCTCGG CGTGAAGGCT 
CTGGTTGGAG GCGCTGTGGG TGGTCTGGCC GGAGCGGCGT CTCGCATCAC TGGAGCGATG 
GCTAAAGGTG TGGCGGCGAT GACGATGGAT GAAGAGTACC AGCAGAAGAG ACGAGAGGCC 
ATGAACAAAC AGCCCAGCGG ACTGAGAGAA GGACTGACAC GAGGAGGAAA AGGACTGGTG 
TCTGGCTTCG TGAGTGGCAT CACGGGCATT GTGACCAAAC CCATTAAAGG AGCTCAGAAG 
GAAGGAGCGG CAGGTTTCTT CAAGGGTATG GGTAAGGGTT TAGTTGGAGC TCTGACCAGA 
CCAACAGGGG GCATCATTGA CATGGCCAGC AGCACCTTCC AGGGCATCAA GAGAGCTGCA 
GAGCTGTCTC AGGATGTGGA GAGTCTGCGC CCTCCACGCT TCATCCACGA GGACGGAGTG 
ATCCGGCCGT ACAGAGAGAG AGAAGGCCTC GGCAGTCAGA TGCTGCAGAG TATTGAAAAC 
GGACGGTTTG CCAAGTACAG GTACTTTGCT CACGCTAAAG TCAACGAGTC TGATTTCCTG 
ATGATCACTA AAAGGGGGAT CTTTTTCGTG ACAAAGGGCA CGTTTGGTCA GTTGACATGT 
GAATGGCAGT ATCTGTTCGA TGAGTTTACT AAAGCACCCA TGATTGTGGA AGGCAGGAGA 
CTGCGCATCG AAGCGAAGGA GAGAGTCAAG TCAGTGTTTC ACGCCAAAGA GTTCGGGAAG 
ATCATCAATT TCAAAACTCC AGAATTAGCA AAGTGGGTTC TTGCCAAGCT TGAAGACGCT 
CGGGAGAATC TGCCCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302586.1
CDS	1..5241
Translation	MITSRMMAMRQDREDSVMVDVSYRQSRDGTRLDTLVQDLYLCASMEFLLTVADVFLKALSDGFSGPPKSNKQPASTSKDPAAPAPPVVAKTEMNVVVKNPEIVFVADLTRAEAPALVMTTQCELVMKSEPSGQCMTAAISDLKIVACPFLREKRENNVTTVLQPCEVFFQSRQSNTEPQCIELSISALTLKVSPIIINTVITIQTALSPAAETPQELESPIPQDLWERRSLRDLKLWFLEEEGEEVSTSVTRLIPQGESLKMSIASICLTLEAGVGHRTVPMLLAKASFQGDVKDWSTLINLHCHLSMEVNYYNEMMGLWEPLLEPLDDEDKDGFRPWTLALKMKKKPVVSSGVSDEVDGAVPDYKTVISFTSKDQLNVTLSKCGLHMLSNLGTAFAEAAKQTAECFQTDQTPFVVKNRLGLAVSVLHSEVFRPIGKESKDRVVELPDGESLNLDYTLTAESSDQFSAMTSLSAKNYIQPTPVGHTTASVIPLIKTGRGMYSVMHNESRVTRFFVCQIYTVEGSKYVKIRSPFQIINHFSVPFRVYEGSVFLGESLPTEEFCVPLGSYRAELSLQPVPDGGEEYEHSESFTYTEVVQKPGKCLEKNCHLRGDSAQILKVNMVPLADTVTSHSSEEEFDKPIVLHLWPTVLLRNLLPYPITFKLKGIGDSPSVSLKEGHSSQIHTAIMDFSVLDLRLLGYLQQDWESQYSLSSEQDEISFIKFRSLAREEETESRPELDIAMHVKKEEGQVVLAVHSPYWMVNKTSRLLQYKADDIHRKHPKDYDMPLLFSFKPRNFLQNNKIRLMISESELSDDFSIDTVGSYGDVKCKGRQKDYLVGVKIDASSFSLTRLVTFLPFYMLVNRTKHVIRVCEEGQENWTEARPGQAAVPFWPERDSKKLRVKVEGSQSSPRAFDFHQPENCLLLHLDKSLGGIIVDVNLTEHSAVILFSDYHDGAAPFLLINHTKDETLQYHQSNSKRKPSTWFQDEILDLSMRDETDSLCSQAEIDELSPGQAVYYTWAEPTGSRILSWKLGKYKGELKSEEDQLLDVDKEGKLFLVSFYEGLQRVALFTSEQRIFKKICDSEKIELAQQEISILLQNMGISLVNNNSSQEVAFIGITSSDVVWETKPKKRSRWKVLSVKEAAVLEQHYRDYMENGPVDSTIIDLDNDYQVLFAANGVDMRIMQPYDAPLRRNFLPALKVEYSVSARQKTYRVQINRIQIQNQLQGAIFPFVFYPIKPPKSVSMDSEPKPLADVSIITRAAGHSDILRIKYFMVLIQEMDLRLDLGFLYAILELFTAEHTSAVTSEQEVELFDKDIEYLKTELNHVSATDTSPISLYEYFHISPIKLHLSLSLSSGGEDSNKQERETELIPVQSLHLLLKSIGATLTDAQDVLFKLGFFELRYQFRTTQQLQWEVVRHYSKQAIKQMYVLVLGLDVLGNPFGLIRGLSEGVEAFFYEPYQGAIQGPEEFMEGMALGVKALVGGAVGGLAGAASRITGAMAKGVAAMTMDEEYQQKRREAMNKQPSGLREGLTRGGKGLVSGFVSGITGIVTKPIKGAQKEGAAGFFKGMGKGLVGALTRPTGGIIDMASSTFQGIKRAAELSQDVESLRPPRFIHEDGVIRPYREREGLGSQMLQSIENGRFAKYRYFAHAKVNESDFLMITKRGIFFVTKGTFGQLTCEWQYLFDEFTKAPMIVEGRRLRIEAKERVKSVFHAKEFGKIINFKTPELAKWVLAKLEDARENLPK

Target ORF information:

RefSeq Version	XM_009304311.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio vacuolar protein sorting 13 homolog A (S. cerevisiae) (vps13a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304311.1

ORF Insert Sequence:

ATGATCACAT CCAGGATGAT GGCGATGCGT CAGGACCGAG AGGACAGTGT GATGGTGGAC 
GTCAGCTACC GCCAGAGTCG GGACGGGACT CGTCTGGACA CCCTCGTGCA AGACCTTTAT 
CTTTGTGCCA GTATGGAGTT TCTGCTCACT GTAGCTGATG TGTTTCTTAA AGCTCTGTCT 
GACGGCTTCT CCGGTCCTCC AAAGAGTAAC AAACAACCGG CCAGCACCTC TAAAGACCCA 
GCGGCTCCTG CTCCACCAGT GGTTGCTAAA ACCGAGATGA ATGTGGTGGT GAAGAACCCA 
GAGATCGTGT TTGTGGCGGA TCTGACGCGT GCTGAAGCTC CGGCTCTGGT CATGACCACT 
CAGTGTGAGC TGGTGATGAA GAGCGAGCCC AGCGGTCAGT GCATGACGGC CGCCATTTCA 
GACCTGAAGA TTGTGGCCTG TCCGTTCCTG CGGGAGAAGA GGGAGAACAA CGTGACGACG 
GTCCTACAGC CCTGCGAGGT TTTCTTCCAG AGCCGACAGT CAAACACTGA GCCGCAGTGC 
ATCGAGCTGT CCATCAGCGC ACTCACGCTC AAGGTGTCTC CGATCATCAT AAACACGGTC 
ATCACCATTC AGACGGCTCT AAGTCCTGCC GCTGAGACGC CGCAGGAGCT GGAGAGCCCC 
ATCCCACAGG ACCTATGGGA GCGCCGCAGC CTTCGCGACC TGAAGCTCTG GTTCCTGGAG 
GAGGAGGGCG AGGAGGTCAG CACGTCCGTC ACGCGTCTCA TTCCTCAGGG AGAGTCTCTG 
AAGATGAGCA TCGCCTCCAT CTGCCTGACG CTGGAGGCCG GAGTGGGACA CCGCACCGTC 
CCCATGCTGC TGGCCAAAGC CTCGTTCCAG GGGGACGTCA AGGACTGGAG CACTCTCATC 
AACCTGCACT GCCACCTCAG CATGGAGGTG AACTACTATA ATGAGATGAT GGGTTTATGG 
GAGCCGCTGT TGGAGCCGCT GGACGATGAG GATAAAGACG GTTTTAGACC CTGGACTCTC 
GCACTGAAGA TGAAGAAGAA GCCGGTGGTC AGTTCTGGGG TGTCTGATGA GGTGGACGGC 
GCTGTGCCTG ATTATAAAAC GGTCATCAGC TTCACTAGTA AAGACCAGCT TAACGTCACC 
CTGTCCAAGT GTGGTCTGCA CATGCTCAGT AATCTGGGAA CGGCTTTTGC AGAGGCCGCT 
AAACAGACGG CCGAGTGCTT TCAGACAGAT CAGACACCAT TCGTGGTGAA GAATCGCCTG 
GGTTTGGCGG TGTCTGTGCT GCACAGCGAA GTGTTTCGAC CCATCGGGAA GGAGTCTAAG 
GATCGTGTGG TGGAGCTCCC GGATGGAGAG AGTCTGAATT TGGACTATAC CCTCACCGCC 
GAGTCCAGCG ACCAGTTCTC TGCCATGACC AGCCTCAGTG CCAAAAACTA CATACAGCCC 
ACTCCAGTCG GCCACACGAC CGCAAGTGTG ATCCCGCTCA TCAAAACGGG CCGGGGCATG 
TACAGCGTCA TGCACAACGA GTCCCGCGTC ACTCGCTTCT TCGTCTGTCA GATTTACACC 
GTCGAGGGCA GCAAATATGT CAAGATTCGC TCTCCCTTCC AGATTATCAA CCACTTTTCA 
GTGCCGTTCC GTGTTTATGA AGGATCCGTG TTTCTGGGCG AGTCTCTGCC GACAGAGGAG 
TTCTGTGTGC CGCTGGGATC CTACAGAGCT GAGCTGAGTC TGCAGCCTGT GCCTGATGGC 
GGTGAAGAGT ACGAGCACTC CGAGAGCTTC ACCTACACTG AGGTTGTCCA GAAACCCGGA 
AAGTGTCTGG AGAAAAACTG CCATCTGCGT GGTGACAGCG CCCAGATTCT GAAGGTCAAC 
ATGGTTCCTC TTGCGGACAC AGTGACGTCC CACAGCAGCG AGGAGGAGTT CGATAAGCCC 
ATCGTACTGC ATTTGTGGCC CACCGTCCTG CTGCGCAACC TGCTGCCCTA CCCAATTACT 
TTCAAGCTAA AGGGCATCGG AGATTCCCCC TCGGTGTCGC TGAAGGAGGG CCACTCATCC 
CAGATCCACA CCGCCATCAT GGACTTCTCC GTGTTGGATC TGAGGCTGCT GGGATACTTG 
CAGCAGGACT GGGAGTCTCA GTACAGCCTC AGCAGTGAAC AAGACGAGAT CAGCTTCATT 
AAGTTTCGCT CGCTCGCACG TGAGGAGGAG ACTGAGAGCC GGCCGGAGCT GGACATCGCT 
ATGCATGTGA AGAAGGAGGA GGGTCAGGTT GTGCTGGCCG TACACTCGCC GTACTGGATG 
GTGAATAAAA CAAGCCGACT GCTGCAGTAC AAGGCGGATG ATATTCATCG CAAACACCCC 
AAGGATTACG ACATGCCGCT GCTCTTCTCC TTCAAGCCAC GCAACTTCCT GCAAAACAAC 
AAGATCCGCC TGATGATCTC AGAAAGTGAG CTGTCAGATG ATTTCTCCAT CGACACTGTC 
GGGAGTTACG GAGATGTCAA ATGCAAAGGC AGACAGAAGG ATTACTTGGT GGGAGTGAAG 
ATCGATGCTA GCAGCTTCAG CTTGACACGT TTGGTGACTT TTCTGCCATT CTACATGCTG 
GTGAACAGAA CCAAACATGT GATTCGGGTC TGTGAGGAGG GGCAGGAGAA CTGGACAGAA 
GCCCGACCCG GACAGGCTGC TGTTCCATTC TGGCCAGAAA GAGACTCAAA GAAGCTGAGA 
GTGAAGGTGG AGGGCAGTCA GTCGTCACCA CGCGCCTTTG ACTTCCACCA GCCAGAGAAC 
TGCCTCCTGC TGCATCTGGA CAAAAGCTTG GGTGGCATCA TTGTGGATGT GAATCTGACA 
GAGCATTCAG CTGTGATCCT CTTCTCTGAT TATCATGATG GAGCCGCTCC CTTCCTCCTC 
ATCAACCACA CTAAGGACGA GACGCTGCAG TACCACCAGA GCAACAGTAA GAGGAAGCCT 
TCGACGTGGT TTCAGGATGA AATACTGGAT TTATCAATGA GGGATGAGAC TGATTCCCTC 
TGCTCTCAGG CCGAGATCGA TGAACTGAGT CCAGGTCAGG CGGTTTACTA CACCTGGGCT 
GAACCAACCG GCTCCAGGAT CCTCAGCTGG AAACTGGGCA AATACAAAGG AGAGCTCAAG 
AGTGAAGAGG ATCAGTTGCT GGATGTGGAC AAGGAGGGCA AGTTGTTTTT GGTGTCGTTT 
TATGAAGGCC TCCAGCGTGT GGCGCTCTTC ACCAGCGAGC AGCGCATCTT CAAGAAGATC 
TGCGACAGTG AGAAGATAGA GCTGGCTCAG CAAGAAATCA GCATCTTGCT GCAGAACATG 
GGCATTTCGC TGGTCAACAA CAACTCCAGC CAGGAAGTGG CCTTCATTGG CATCACCAGC 
TCTGATGTTG TGTGGGAAAC CAAGCCGAAG AAGCGCAGCC GCTGGAAAGT TCTGAGTGTT 
AAAGAGGCAG CAGTTCTGGA GCAGCACTAC AGAGACTACA TGGAGAACGG CCCAGTGGAC 
AGCACCATCA TAGACCTGGA CAACGACTAC CAGGTGTTGT TTGCTGCTAA CGGTGTGGAC 
ATGAGGATCA TGCAGCCCTA CGATGCTCCA CTCAGAAGGA ACTTCCTGCC GGCGTTGAAG 
GTGGAGTACA GCGTTTCAGC CCGCCAGAAA ACATACAGAG TCCAGATCAA CAGGATCCAG 
ATCCAAAACC AGCTGCAAGG AGCCATATTC CCGTTTGTGT TTTACCCCAT CAAACCGCCA 
AAGTCTGTCA GCATGGACTC AGAGCCAAAA CCGCTGGCTG ACGTGAGCAT CATCACCAGA 
GCAGCAGGAC ACTCCGACAT TCTGCGCATC AAATATTTTA TGGTGTTGAT TCAGGAGATG 
GACCTGCGGC TGGATCTGGG CTTTCTGTAT GCCATACTTG AGCTCTTCAC TGCTGAACAC 
ACCAGTGCTG TGACGTCTGA ACAGGAGGTG GAGTTGTTTG ATAAAGACAT AGAGTATCTA 
AAGACTGAGT TAAATCATGT TTCAGCTACA GACACATCTC CCATCAGCCT CTACGAGTAC 
TTCCACATCT CTCCTATAAA GCTTCACTTG AGCTTGTCTC TGAGCAGTGG TGGTGAAGAC 
AGCAATAAGC AAGAGCGCGA GACGGAGCTG ATCCCAGTTC AGTCTCTCCA TCTGCTGCTC 
AAGAGCATCG GGGCTACACT TACAGACGCA CAGGACGTCC TCTTTAAACT GGGCTTCTTC 
GAGCTGAGGT ATCAGTTTCG CACCACTCAG CAGCTGCAGT GGGAGGTCGT TCGCCATTAC 
TCCAAACAGG CTATCAAGCA GATGTATGTC CTTGTTCTGG GTTTGGACGT TCTTGGGAAT 
CCATTCGGGC TGATCCGGGG CCTGTCTGAA GGCGTCGAGG CTTTCTTTTA CGAGCCCTAT 
CAGGGTGCAA TTCAGGGTCC AGAGGAGTTC ATGGAGGGCA TGGCTCTCGG CGTGAAGGCT 
CTGGTTGGAG GCGCTGTGGG TGGTCTGGCC GGAGCGGCGT CTCGCATCAC TGGAGCGATG 
GCTAAAGGTG TGGCGGCGAT GACGATGGAT GAAGAGTACC AGCAGAAGAG ACGAGAGGCC 
ATGAACAAAC AGCCCAGCGG ACTGAGAGAA GGACTGACAC GAGGAGGAAA AGGACTGGTG 
TCTGGCTTCG TGAGTGGCAT CACGGGCATT GTGACCAAAC CCATTAAAGG AGCTCAGAAG 
GAAGGAGCGG CAGGTTTCTT CAAGGGTATG GGTAAGGGTT TAGTTGGAGC TCTGACCAGA 
CCAACAGGGG GCATCATTGA CATGGCCAGC AGCACCTTCC AGGGCATCAA GAGAGCTGCA 
GAGCTGTCTC AGGATGTGGA GAGTCTGCGC CCTCCACGCT TCATCCACGA GGACGGAGTG 
ATCCGGCCGT ACAGAGAGAG AGAAGGCCTC GGCAGTCAGA TGCTGCAGAG TATTGAAAAC 
GGACGGTTTG CCAAGTACAG GTACTTTGCT CACGCTAAAG TCAACGAGTC TGATTTCCTG 
ATGATCACTA AAAGGGGGAT CTTTTTCGTG ACAAAGGGCA CGTTTGGTCA GTTGACATGT 
GAATGGCAGT ATCTGTTCGA TGAGTTTACT AAAGCACCCA TGATTGTGGA AGGCAGGAGA 
CTGCGCATCG AAGCGAAGGA GAGAGTCAAG TCAGTGTTTC ACGCCAAAGA GTTCGGGAAG 
ATCATCAATT TCAAAACTCC AGAATTAGCA AAGTGGGTTC TTGCCAAGCT TGAAGACGCT 
CGGGAGAATC TGCCCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS13A ( NM_001118893.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001112365.1 Danio rerio vacuolar protein sorting 13 homolog A (vps13a), mRNA.

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CloneID	ODa34180
Clone ID Related Accession (Same CDS sequence)	NM_001118893.2
Accession Version	NM_001118893.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1554566400000
Organism	Danio rerio(zebrafish)
Product	vacuolar protein sorting-associated protein 13A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX005248.13. On Jul 23, 2009 this sequence version replaced NM_001118893.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGTGTTTG AGTCGGTGGT GGTCGACGTG CTGAACCGGT TTCTGGGGGA CTATGTTGGA 
AACCTCGACA GCTCGCAGCT CAGCCTCGGC ATATGGGGAG GTGATGCCAT ATTGAGGAAC 
CTCGAGATTA AAGAAAATGC ATTGAGCCAG CTGGACATTC CTTTCAAAGT CAGAGCAGGA 
CACATTGGGC GTTTGGAGTT AAAGATCCCC TGGAAGAACC TGTACACTCA GTCTGTGGAG 
GCCACGCTGG ATGAAGTTTA CCTGCTCATC GTACCTACTG CTAGTATAAA GTATGACGCA 
GAGAAGGAGG AGCGGCAGCT GCAGGAGTCT CGGCAGAGGG AGCTCCAGCG CATCGAGGAG 
ACCAAACAGA AAGCAGCAGA AAAAGAGAAT CCAAAGGGTG AGAAGCAGGA CACATTTGTG 
GAGAAACTGG TAACACAGGT GATCAAAAAC CTGCAGGTCA AAATATCCAA CATTCACGTC 
CGCTATGAGG ATGATGTCAC CAATCCCAGT GTTCCTCTCT CCTTTGGGAT ATCATTACAA 
AACCTCAGTC TCCAGACGGC GGATCAGAAC TGGAACCCCT GTCTTCTGGA TGAGAAAGCC 
AAACTGTTTT TTAAGCTTGT ACGTCTGGAT AATCTGTTTG CGTACTGGAA CGTCAGTTCA 
GAGCTGTACT CCAACCACAA CCCTGAGGAG GCTCTGCGAT ACCTGAAGCA CAGCGTAGCC 
ATCCAGACTG CCATCCCTCA GGATTATCAC TTCATCTTTC GTCCAATATC TGCCAATGCC 
AAGTTGCGGA TGAACCCTCG CTCTGATGTA GACTTCTCCT CACCCAAAAT GGACCTGGTG 
GTCAACCTTC CTGAAGTGGC CGTGGAGCTC AGCCGACCAC AGTACGTCAG CATTCTGGAG 
CTGTTGGGGT CGGTGGATAT GATGACCCGT AACCTTCCTT ACAGGAAGTA TCGGCCATAT 
GTGGTGGTTC ACACTAACGC TCGTCAGTGG TGGCGATATG TGATCACGGG TGTTCTGGAG 
GTCAATGTGA AGCCTCGTCT TCACATGTGG TCTTGGCTGC ATATTCGTCG ACATCGGCAG 
CATGTGAAAA AGTACAGAGA GCTTTATAAG AATAAAATCA TCAGTAAGAA ACCGGCAGAG 
AGTCTGCTGA AGGAGCTGGA GGAGACTGAA AGAACATTAG ACATATTCAA CATCACAATG 
GCGAGACAGC AGGCTGAGAT GGAGGCCTCG AAGGCAGGGC TCAGGATTTA CCGGCCCGGG 
GTGAAGGTGG AGGAGGAACA GTCACAGGGC TGGTTTGGCT GGATGTGGAA CTGGTCTGGA 
GAAGCCAGTG CCACTCAAAC CAAAGACGTC AAAACTGGAG GTTTTGATGG GTTAATGACG 
CCTGCAGAGA AAGCCAAACT ATACGCTGCC ATCGGATACA GCGAAACTGC TGTCAACCCC 
AACCTGCCAA AAAATTTTGA AAACATGAAG ATGAATTTCC ATCTTCTCCG CCTGTCCCTG 
TCCATCAAAG AGAGCCGAGA GAAGGCGGAG ATCATCAAAC TGTCTGTCAT TGACCTGAAG 
GCCATGCTGA CTCAAAGACC CGCGGCTCAA GCTATAAAGA TCTCCGCTCA GCTGTCGTCG 
TTTGAAGTGT CCGGTCTCCC GCGGGAAAAG TCGGCCCCGA TCCTGCTTTC TCCACGTCAT 
GTGGACGGCA TGAAGGATAA AAGTCTGCTG AACCTCATGT TCGAGACCAA CCCGCTGGAC 
GCTCGTGCTG ACCAGAGACT GCGCATCGAG TCCCAGCCGC TGGAGATCAT CTATGATGCA 
ATGACCGTGA ACAGTATGTC CGCTTTCTTC ATGCCTCCTA AAGACCTGCA GCTGGATGAA 
CTGACCAACG CCACACTGAT GAAGCTGGAG CAGTTCAGAG ACAAAACCTC CACAGGCCTC 
ATGTACATCA TTGAGACGCA GAAAGTTCTG GATCTGAAAA TAAACCTCAT GGCCTCGTAT 
GTCATCGTAC CGGAAAACGG CTTTTATGAC CCCAAACACA ACCTTGCCAT TCTAGATCTG 
GGTCATTTTC AGATGTCAAG TCAGAGCCGA GCTGATCTTC CTCAGCTGTC TGCGGGCAGC 
AGTACTCTAG AGGACATCAT GTCTCGAGCG TACGACAGCT TCGACGTCCA GCTCAGCAGT 
CTGCAGTTCC TCTACTCCAA AGCAGGTGGA GACTGGAAGC GGGCTCGTCC GCTAAGACAG 
TCTGCTTTCC ACATCCTGGA GCCAGTGGAC CTGAAGGTGG TCTTTAGCAG AGCGATGGTG 
GTCACAGACT CCAGGATGCC CAAGTTTAAG ATGCACGGTG AGCTGCCTCT GCTGTCTCTG 
CGGATCTCTG ATGATAAAGT GCGTGGCGTT CTGGCTCTGC TCAGCAGTAT TCCTCTACCT 
GAGAGCCGGC CCACGCCTCA GTCCAGAAAC ACGCAGGCCA CTCCAAAGGC CTCCAAGCGA 
GGAAATACCC CTCAGCTCAC GCCCCGAAGG CCAGCTATAG ATGACCTGCG TGCGTCCATC 
ATATACGGCT CAGAGGAGGA GCTGTTCTTT GACGCCCCCA GTTCACCGAT CGAGGATCTG 
CCGTTCTTCG AGGCCTCGTC GGACTCTCGG CATGCTTCAG CAGCTGATAA ACGTCTGTCT 
ATGGTGCTCA AAGACCCCAA GAAGAACATG ACTGAGTTTC TGCTTAGTTT TGAGATCAAC 
AAGCTCTCGG TGCAGCTGTG CCGTGTGTCG AATAGACAGG AAGTGAGTGT TCTGCATCTG 
TTCATCGATG GATTGGGAAC AGAGCTCAAA CTTCGCACGT TTGACATGTC GTCGTACACT 
TTCCTGCGTG AGATCTGCCT CGAGTGCTCA GAATACTTGG ATTCTGAGGA CAAGAAAGTT 
CACCTTATCA CCACGCTGGA CAACACAGAG GAGGATCTGC TCACTCTGGA GTATGTCAAG 
GCAGACAAAA ATGCACCAGA TTTTAAAGCT ATGTACAAAA ATACAGAGCA ACTAATCAAG 
GTGAATTTCT CCTCTCTGGA CATTCACCTG CACACAGAGG CTCTCCTGAA CTCCATGAAC 
TTCCTAAATA ATCTTCTGCC ACCGATGAAG AAGGAGACCC AGGAGGAACA ACCAATAATT 
CCCAAGGAGG AAGAGGAGGA GGAGGAGGAA GAAGAAGAAG AGGAGGAGGC TAAAGTAGAG 
AATTCTGCTT TTGAGGATGT GGTGAATTTG CACATCCGCG CAGACCTCAA CTGTCTGAAG 
GTGTTCATAC GAGGAGAGAA AGCCAGGATC TCTGAGATCA GCATAGAGGG ACTGGTTTCT 
GAAGTTCAGA TGAAGAAGAA GTCTGTGGAG ATTCTGGCTA ACTTGAAGAA CATCATCATT 
CTGGATTGTG ACAAAGAGGC ACTTTATAAA AAGGCAGTGT CCATCGCTGA TAAGGAGGTG 
TTTGATTTCC GCATGGTGAA CCATGTCGAT GCAACGGAGG GTGACGCTTA CACAGACATG 
CACATTGTGG ACACTAGCGT AACCCTGACC GTGGGCTGCA TTCAAGTTAT TTTCCTCAAC 
AAGTTTATGT CCTCAATACT GGCATTTATT AATAATTTCC AGGAGGCTAA AAATGCATTG 
GCGGAGGTGA CGGCCCAGGC AGCAGAGAAA GCAGCCACGG GGGTTCTGGA GCTGGCAGAG 
AGAAGTACTC GGATCTCCCT GGACGTCGAC ATCAAAGCTC CTGTTGTCTT CCTCCCACAA 
TCCTCAGTTT CCCACAACGT CATTGTGGCA GATCTGGGTT TAGTGACTGT AAAGAACCGC 
TTTGAGATCG TCTCCTCTCC AACACACACC AAGATCCCTC CTGTCATGGA CATCATGGCT 
GTCGGATTGA GTGATCTGAA AATGTACAGG ACAACGTTCA CTGATGACAA GTTTAACAGC 
GAGATTCAGT TGCTCAAGCC AGTCAACATG GACCTGTCAA TCAAACGCAA TCTCTCCGCC 
TCCTGGTATC ACAGCATACC CGACATCCAG ATCAATGCTC ACCTCAAGCC CATGAGCCTG 
ATCTTGAGTC AGGAGGATCT GACTGTGGTG CTGAAGACTC TGAGTGAGAA TCTGTCCGAG 
ACGTCTGATG CTCCTCCAGC TCCGTCCAGC TCTGATAAAG GAGATACGGT TTCTAACAAA 
GGCTCCCAGG GTTCTGGAGG CAACACCGTT GTGACCGCAG CAGTGGTGGA GGGTCAGAAA 
TCAGGCAAAC TGAAGAACAC CCTAAAACTG AACTTCAAGT TTGACTCCAT GACGCTGGTC 
CTCTACAGCC CGAACAGCGA GCAAGCGAAA TTGTCCGACT CTCGTGATGA GAGTTTGAAG 
TTGGCCGAGT TCACACTGGG CACCATCTCC ACCAGTGTGC ACATGTTCTC CGACAGCTCC 
ATGAAGGCCT CGCTCAAACT CACCAACTGT CTGCTGGATG ACAAGAGGCA GGGAGTGAAG 
ATGATTACAT CCAGGATGAT GGCGATGCGT CAGGACCGAG AGGACAGTGT GATGGTGGAC 
GTCAGCTACC GCCAGAGTCG GGACGGGACT CGTCTGGACA CCCTCGTGCA AGACCTTTAT 
CTTTGTGCCA GTATGGAGTT TCTGCTCACT GTAGCTGATG TGTTTCTTAA AGCTCTGTCT 
GACGGCTTCT CCGGTCCTCC AAAGATTGCT CTGTGGCTGC TGAAATGCTT GGCCCCCGAT 
AAATTGGTTG CTAAAACCGA GATGAATGTG GTGGTGAAGA ACCCAGAGAT CGTGTTTGTG 
GCGGATCTGA CGCGTGCTGA AGCTCCGGCT CTGGTCATGA CCACTCAGTG TGAGCTGGTG 
ATGAAGAGCG AGCCCAGCGG TCAGTGCATG ACGGCCGCCA TTTCAGACCT GAAGATTGTG 
GCCTGTCCGT TCCTGCGGGA GAAGAGGGAG AACAACGTGA CGACGGTCCT ACAGCCCTGC 
GAGGTTTTCT TCCAGAGCCG ACAGTCAAAC ACTGAGCCGC AGTGCATCGA GCTGTCCATC 
AGCGCACTCA CGCTCAAGGT GTCTCCGATC ATCATAAACA CGGTCATCAC CATTCAGACG 
GCTCTAAGTC CTGCCGCTGA GACGCCGCAG GAGCTGGAGA GCCCCATCCC ACAGGACCTA 
TGGGAGCGCC GCAGCCTTCG CGACCTGAAG CTCTGGTTCC TGGAGGAGGA GGGCGAGGAG 
GTCAGCACGT CCGTCACGCG TCTCATTCCT CAGGGAGAGT CTCTGAAGAT GAGCATCGCC 
TCCATCTGCC TGACGCTGGA GGCCGGAGTG GGACACCGCA CCGTCCCCAT GCTGCTGGCC 
AAAGCCTCGT TCCAGGGGGA CGTCAAGGAC TGGAGCACTC TCATCAACCT GCACTGCCAC 
CTCAGCATGG AGGTGAACTA CTATAATGAG ATGATGGGTT TATGGGAGCC GCTGTTGGAG 
CCGCTGGACG ATGAGGATAA AGACGGTTTT AGACCCTGGA CTCTCGCACT GAAGATGAAG 
AAGAAGCCGG TGGTCAGTTC TGGGGTGTCT GATGAGGTGG ACGGCGCTGT GCCTGATTAT 
AAAACGGTCA TCAGCTTCAC TAGTAAAGAC CAGCTTAACG TCACCCTGTC CAAGTGTGGT 
CTGCACATGC TCAGTAATCT GGGAACGGCT TTTGCAGAGG CCGCTAAACA GACGGCCGAG 
TGCTTTCAGA CAGATCAGAC ACCATTCGTG GTGAAGAATC GCCTGGGTTT GGCGGTGTCT 
GTGCTGCACA GCGAAGTGTT TCGACCCATC GGGAAGGAGT CTAAGGATCG TGTGGTGGAG 
CTCCCGGATG GAGAGAGTCT GAATTTGGAC TATACCCTCA CCGCCGAGTC CAGCGACCAG 
TTCTCTGCCA TGACCAGCCT CAGTGCCAAA AACTACATAC AGCCCACTCC AGTCGGCCAC 
ACGACCGCAA GTGTGATCCC GCTCATCAAA ACGGGCCGGG GCATGTACAG CGTCATGCAC 
AACGAGTCCC GCGTCACTCG CTTCTTCGTC TGTCAGATTT ACACCGTCGA GGGCAGCAAA 
TATGTCAAGA TTCGCTCTCC CTTCCAGATT ATCAACCACT TTTCAGTGCC GTTCCGTGTT 
TATGAAGGAT CCGTGTTTCT GGGCGAGTCT CTGCCGACAG AGGAGTTCTG TGTGCCGCTG 
GGATCCTACA GGTCTCTTAC AGCATTTCTG CAGCCTGTGC CTGATGGCGG TGAAGAGTAC 
GAGCACTCCG AGAGCTTCAC CTACACTGAG GTTGTCCAGA AACCCGGAAA GTGTCTGGAG 
AAAAACTGCC ATCTGCGTGG TGACAGCGCC CAGATTCTGA AGGTCAACAT GGTTCCTCTT 
GCGGACACAG TGACGTCCCA CAGCAGCGAG GAGGAGTTCG ATAAGCCCAT CGTACTGCAT 
TTGTGGCCCA CCGTCCTGCT GCGCAACCTG CTGCCCTACC CAATTACTTT CAAGCTAAAG 
GGCATCGGAG ATTCCCCCTC GGTGTCGCTG AAGGAGGGCC ACTCATCCCA GATCCACACC 
GCCATCATGG ACTTCTCCGT GTTGGATCTG AGGCTGCTGG GATACTTGCA GCAGGACTGG 
GAGTCTCAGT ACAGCCTCAG CAGTGAACAA GACGAGATCA GCTTCATTAA GTTTCGCTCG 
CTCGCACGTG AGGAGGAGAC TGAGAGCCGG CCGGAGCTGG ACATCGCTAT GCATGTGAAG 
AAGGAGGAGG GTCAGGTTGT GCTGGCCGTA CACTCGCCGT ACTGGATGGT GAATAAAACA 
AGCCGACTGC TGCAGTACAA GGCGGATGAT ATTCATCGCA AACACCCCAA GGATTACGAC 
ATGCCGCTGC TCTTCTCCTT CAAGCCACGC AACTTCCTGC AAAACAACAA GATCCGCCTG 
ATGATCTCAG AAAGTGAGCT GTCAGATGAT TTCTCCATCG ACACTGTCGG GAGTTACGGA 
GATGTCAAAT GCAAAGGCAG ACAGAAGGAT TACTTGGTGG GAGTGAAGAT CGATGCTAGC 
AGCTTCAGCT TGACACGTTT GGTGACTTTT CTGCCATTCT ACATGCTGGT GAACAGAACC 
AAACATGTGA TTCGGGTCTG TGAGGAGGGG CAGGAGAACT GGTTAAGTTC TTCTGTTCAG 
GCTGCTGTTC CATTCTGGCC AGAAAGAGAC TCAAAGAAGC TGAGAGTGAA GGTGGAGGGC 
AGTCAGTCGT CACCACGCGC CTTTGACTTC CACCAGCCAG AGAACTGCCT CCTGCTGCAT 
CTGGACAAAA GCTTGGGTGG CATCATTGTG GATGTGAATC TGACAGAGCA TTCAGCTGTG 
ATCCTCTTCT CTGATTATCA TGATGGAGCC GCTCCCTTCC TCCTCATCAA CCACACTAAG 
GACGAGACGC TGCAGTACCA CCAGAGCTCT CAGGCCGAGA TCGATGAACT GAGTCCAGGT 
CAGGCGGTTT ACTACACCTG GGCTGAACCA ACCGGCTCCA GGATCCTCAG CTGGAAACTG 
GGCAAATACA AAGGAGAGCT CAAGAGTGAA GAGGATCAGT TGCTGGATGT GGACAAGGAG 
GGCAAGTTGT TTTTGGTGTC GTTTTATGAA GGCCTCCAGC GTGTGGCGCT CTTCACCAGC 
GAGCAGCGCA TCTTCAAGAA GATCTGCGAC AGTGAGAAGA TAGAGCTGGC TCAGCAAGAA 
ATCAGCATCT TGCTGCAGAA CATGGGCATT TCGCTGGTCA ACAACAACTC CAGCCAGGAA 
GTGGCCTTCA TTGGCATCAC CAGCTCTGAT GTTGTGTGGG AAACCAAGCC GAAGAAGCGC 
AGCCGCTGGA AAGTTCTGAG TGTTAAAGAG GCAGCAGTTC TGGAGCAGCA CTACAGAGAC 
TACATGGAGA ACGGCCCAGT GGACAGCACC ATCATAGACC TGGACAACGA CTACCAGGTG 
TTGTTTGCTG CTAACGGTGT GGACATGAGG ATCATGCAGC CCTACGATGC TCCACTCAGA 
AGGAACTTCC TGCCGGCGTT GAAGGTGGAG TACAGCGTTT CAGCCCGCCA GAAAACATAC 
AGAGTCCAGA TCAACAGGAT CCAGATCCAA AACCAGCTGC AAGGAGCCAT ATTCCCGTTT 
GTGTTTTACC CCATCAAACC GCCAAAGTCT GTCAGCATGG ACTCAGAGCC AAAACCGCTG 
GCTGACGTGA GCATCATCAC CAGAGCAGCA GGACACTCCG ACATTCTGCG CATCAAATAT 
TTTATGGTGT TGATTCAGGA GATGGACCTG CGGCTGGATC TGGGCTTTCT GTATGCCATA 
CTTGAGCTCT TCACTGCTGA ACACACCAGT GCTGTGACGT CTGAACAGGA GGTGGAGTTG 
TTTGATAAAG ACATAGAGTA TCTAAAGACT GAGTTAAATC ATGTTTCAGC TACAGACACA 
TCTCCCATCA GCCTCTACGA GTACTTCCAC ATCTCTCCTA TAAAGCTTCA CTTGAGCTTG 
TCTCTGAGCA GTGGTGGTGA AGACAGCAAT AAGCAAGAGC GCGAGACGGA GCTGATCCCA 
GTTCAGTCTC TCCATCTGCT GCTCAAGAGC ATCGGGGCTA CACTTACAGA CGCACAGGAC 
GTCCTCTTTA AACTGGGCTT CTTCGAGCTG AGGTATCAGT TTCGCACCAC TCAGCAGCTG 
CAGTGGGAGG TCGTTCGCCA TTACTCCAAA CAGGCTATCA AGCAGATGTA TGTCCTTGTT 
CTGGGTTTGG ACGTTCTTGG GAATCCATTC GGGCTGATCC GGGGCCTGTC TGAAGGCGTC 
GAGGCTTTCT TTTACGAGCC CTATCAGGGT GCAATTCAGG GTCCAGAGGA GTTCATGGAG 
GGCATGGCTC TCGGCGTGAA GGCTCTGGTT GGAGGCGCTG TGGGTGGTCT GGCCGGAGCG 
GCGTCTCGCA TCACTGGAGC GATGGCTAAA GGTGTGGCGG CGATGACGAT GGATGAAGAG 
TACCAGCAGA AGAGACGAGA GGCCATGAAC AAACAGCCCA GCGGACTGAG AGAAGGACTG 
ACACGAGGAG GAAAAGGACT GGTGTCTGGC TTCGTGAGTG GCATCACGGG CATTGTGACC 
AAACCCATTA AAGGAGCTCA GAAGGAAGGA GCGGCAGGTT TCTTCAAGGG TATGGGTAAG 
GGTTTAGTTG GAGCTCTGAC CAGACCAACA GGGGGCATCA TTGACATGGC CAGCAGCACC 
TTCCAGGGCA TCAAGAGAGC TGCAGAGCTG TCTCAGGATG TGGAGAGTCT GCGCCCTCCA 
CGCTTCATCC ACGAGGACGG AGTGATCCGG CCGTACAGAG AGAGAGAAGG CCTCGGCAGT 
CAGATGCTGC AGAGTATTGA AAACGGACGG TTTGCCAAGT ACAGGTACTT TGCTCACGCT 
AAAGTCAACG AGTCTGATTT CCTGATGATC ACTAAAAGGG GGATCTTTTT CGTGACAAAG 
GGCACGTTTG GTCAGTTGAC ATGTGAATGG CAGTATCTGT TCGATGAGTT TACTAAAGCA 
CCCATGATTG TGGAAGGCAG GAGACTGCGC ATCGAAGCGA AGGAGAGAGT CAAGTCAGTG 
TTTCACGCCA AAGAGTTCGG GAAGATCATC AATTTCAAAA CTCCAGAATT AGCAAAGTGG 
GTTCTTGCCA AGCTTGAAGA CGCTCGGGAG AATCTGCCCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001112365.1
CDS	51..9635
Translation	MVFESVVVDVLNRFLGDYVGNLDSSQLSLGIWGGDAILRNLEIKENALSQLDIPFKVRAGHIGRLELKIPWKNLYTQSVEATLDEVYLLIVPTASIKYDAEKEERQLQESRQRELQRIEETKQKAAEKENPKGEKQDTFVEKLVTQVIKNLQVKISNIHVRYEDDVTNPSVPLSFGISLQNLSLQTADQNWNPCLLDEKAKLFFKLVRLDNLFAYWNVSSELYSNHNPEEALRYLKHSVAIQTAIPQDYHFIFRPISANAKLRMNPRSDVDFSSPKMDLVVNLPEVAVELSRPQYVSILELLGSVDMMTRNLPYRKYRPYVVVHTNARQWWRYVITGVLEVNVKPRLHMWSWLHIRRHRQHVKKYRELYKNKIISKKPAESLLKELEETERTLDIFNITMARQQAEMEASKAGLRIYRPGVKVEEEQSQGWFGWMWNWSGEASATQTKDVKTGGFDGLMTPAEKAKLYAAIGYSETAVNPNLPKNFENMKMNFHLLRLSLSIKESREKAEIIKLSVIDLKAMLTQRPAAQAIKISAQLSSFEVSGLPREKSAPILLSPRHVDGMKDKSLLNLMFETNPLDARADQRLRIESQPLEIIYDAMTVNSMSAFFMPPKDLQLDELTNATLMKLEQFRDKTSTGLMYIIETQKVLDLKINLMASYVIVPENGFYDPKHNLAILDLGHFQMSSQSRADLPQLSAGSSTLEDIMSRAYDSFDVQLSSLQFLYSKAGGDWKRARPLRQSAFHILEPVDLKVVFSRAMVVTDSRMPKFKMHGELPLLSLRISDDKVRGVLALLSSIPLPESRPTPQSRNTQATPKASKRGNTPQLTPRRPAIDDLRASIIYGSEEELFFDAPSSPIEDLPFFEASSDSRHASAADKRLSMVLKDPKKNMTEFLLSFEINKLSVQLCRVSNRQEVSVLHLFIDGLGTELKLRTFDMSSYTFLREICLECSEYLDSEDKKVHLITTLDNTEEDLLTLEYVKADKNAPDFKAMYKNTEQLIKVNFSSLDIHLHTEALLNSMNFLNNLLPPMKKETQEEQPIIPKEEEEEEEEEEEEEEAKVENSAFEDVVNLHIRADLNCLKVFIRGEKARISEISIEGLVSEVQMKKKSVEILANLKNIIILDCDKEALYKKAVSIADKEVFDFRMVNHVDATEGDAYTDMHIVDTSVTLTVGCIQVIFLNKFMSSILAFINNFQEAKNALAEVTAQAAEKAATGVLELAERSTRISLDVDIKAPVVFLPQSSVSHNVIVADLGLVTVKNRFEIVSSPTHTKIPPVMDIMAVGLSDLKMYRTTFTDDKFNSEIQLLKPVNMDLSIKRNLSASWYHSIPDIQINAHLKPMSLILSQEDLTVVLKTLSENLSETSDAPPAPSSSDKGDTVSNKGSQGSGGNTVVTAAVVEGQKSGKLKNTLKLNFKFDSMTLVLYSPNSEQAKLSDSRDESLKLAEFTLGTISTSVHMFSDSSMKASLKLTNCLLDDKRQGVKMITSRMMAMRQDREDSVMVDVSYRQSRDGTRLDTLVQDLYLCASMEFLLTVADVFLKALSDGFSGPPKIALWLLKCLAPDKLVAKTEMNVVVKNPEIVFVADLTRAEAPALVMTTQCELVMKSEPSGQCMTAAISDLKIVACPFLREKRENNVTTVLQPCEVFFQSRQSNTEPQCIELSISALTLKVSPIIINTVITIQTALSPAAETPQELESPIPQDLWERRSLRDLKLWFLEEEGEEVSTSVTRLIPQGESLKMSIASICLTLEAGVGHRTVPMLLAKASFQGDVKDWSTLINLHCHLSMEVNYYNEMMGLWEPLLEPLDDEDKDGFRPWTLALKMKKKPVVSSGVSDEVDGAVPDYKTVISFTSKDQLNVTLSKCGLHMLSNLGTAFAEAAKQTAECFQTDQTPFVVKNRLGLAVSVLHSEVFRPIGKESKDRVVELPDGESLNLDYTLTAESSDQFSAMTSLSAKNYIQPTPVGHTTASVIPLIKTGRGMYSVMHNESRVTRFFVCQIYTVEGSKYVKIRSPFQIINHFSVPFRVYEGSVFLGESLPTEEFCVPLGSYRSLTAFLQPVPDGGEEYEHSESFTYTEVVQKPGKCLEKNCHLRGDSAQILKVNMVPLADTVTSHSSEEEFDKPIVLHLWPTVLLRNLLPYPITFKLKGIGDSPSVSLKEGHSSQIHTAIMDFSVLDLRLLGYLQQDWESQYSLSSEQDEISFIKFRSLAREEETESRPELDIAMHVKKEEGQVVLAVHSPYWMVNKTSRLLQYKADDIHRKHPKDYDMPLLFSFKPRNFLQNNKIRLMISESELSDDFSIDTVGSYGDVKCKGRQKDYLVGVKIDASSFSLTRLVTFLPFYMLVNRTKHVIRVCEEGQENWLSSSVQAAVPFWPERDSKKLRVKVEGSQSSPRAFDFHQPENCLLLHLDKSLGGIIVDVNLTEHSAVILFSDYHDGAAPFLLINHTKDETLQYHQSSQAEIDELSPGQAVYYTWAEPTGSRILSWKLGKYKGELKSEEDQLLDVDKEGKLFLVSFYEGLQRVALFTSEQRIFKKICDSEKIELAQQEISILLQNMGISLVNNNSSQEVAFIGITSSDVVWETKPKKRSRWKVLSVKEAAVLEQHYRDYMENGPVDSTIIDLDNDYQVLFAANGVDMRIMQPYDAPLRRNFLPALKVEYSVSARQKTYRVQINRIQIQNQLQGAIFPFVFYPIKPPKSVSMDSEPKPLADVSIITRAAGHSDILRIKYFMVLIQEMDLRLDLGFLYAILELFTAEHTSAVTSEQEVELFDKDIEYLKTELNHVSATDTSPISLYEYFHISPIKLHLSLSLSSGGEDSNKQERETELIPVQSLHLLLKSIGATLTDAQDVLFKLGFFELRYQFRTTQQLQWEVVRHYSKQAIKQMYVLVLGLDVLGNPFGLIRGLSEGVEAFFYEPYQGAIQGPEEFMEGMALGVKALVGGAVGGLAGAASRITGAMAKGVAAMTMDEEYQQKRREAMNKQPSGLREGLTRGGKGLVSGFVSGITGIVTKPIKGAQKEGAAGFFKGMGKGLVGALTRPTGGIIDMASSTFQGIKRAAELSQDVESLRPPRFIHEDGVIRPYREREGLGSQMLQSIENGRFAKYRYFAHAKVNESDFLMITKRGIFFVTKGTFGQLTCEWQYLFDEFTKAPMIVEGRRLRIEAKERVKSVFHAKEFGKIINFKTPELAKWVLAKLEDARENLPK

Target ORF information:

RefSeq Version	NM_001118893.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio vacuolar protein sorting 13 homolog A (vps13a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001118893.2

ORF Insert Sequence:

ATGGTGTTTG AGTCGGTGGT GGTCGACGTG CTGAACCGGT TTCTGGGGGA CTATGTTGGA 
AACCTCGACA GCTCGCAGCT CAGCCTCGGC ATATGGGGAG GTGATGCCAT ATTGAGGAAC 
CTCGAGATTA AAGAAAATGC ATTGAGCCAG CTGGACATTC CTTTCAAAGT CAGAGCAGGA 
CACATTGGGC GTTTGGAGTT AAAGATCCCC TGGAAGAACC TGTACACTCA GTCTGTGGAG 
GCCACGCTGG ATGAAGTTTA CCTGCTCATC GTACCTACTG CTAGTATAAA GTATGACGCA 
GAGAAGGAGG AGCGGCAGCT GCAGGAGTCT CGGCAGAGGG AGCTCCAGCG CATCGAGGAG 
ACCAAACAGA AAGCAGCAGA AAAAGAGAAT CCAAAGGGTG AGAAGCAGGA CACATTTGTG 
GAGAAACTGG TAACACAGGT GATCAAAAAC CTGCAGGTCA AAATATCCAA CATTCACGTC 
CGCTATGAGG ATGATGTCAC CAATCCCAGT GTTCCTCTCT CCTTTGGGAT ATCATTACAA 
AACCTCAGTC TCCAGACGGC GGATCAGAAC TGGAACCCCT GTCTTCTGGA TGAGAAAGCC 
AAACTGTTTT TTAAGCTTGT ACGTCTGGAT AATCTGTTTG CGTACTGGAA CGTCAGTTCA 
GAGCTGTACT CCAACCACAA CCCTGAGGAG GCTCTGCGAT ACCTGAAGCA CAGCGTAGCC 
ATCCAGACTG CCATCCCTCA GGATTATCAC TTCATCTTTC GTCCAATATC TGCCAATGCC 
AAGTTGCGGA TGAACCCTCG CTCTGATGTA GACTTCTCCT CACCCAAAAT GGACCTGGTG 
GTCAACCTTC CTGAAGTGGC CGTGGAGCTC AGCCGACCAC AGTACGTCAG CATTCTGGAG 
CTGTTGGGGT CGGTGGATAT GATGACCCGT AACCTTCCTT ACAGGAAGTA TCGGCCATAT 
GTGGTGGTTC ACACTAACGC TCGTCAGTGG TGGCGATATG TGATCACGGG TGTTCTGGAG 
GTCAATGTGA AGCCTCGTCT TCACATGTGG TCTTGGCTGC ATATTCGTCG ACATCGGCAG 
CATGTGAAAA AGTACAGAGA GCTTTATAAG AATAAAATCA TCAGTAAGAA ACCGGCAGAG 
AGTCTGCTGA AGGAGCTGGA GGAGACTGAA AGAACATTAG ACATATTCAA CATCACAATG 
GCGAGACAGC AGGCTGAGAT GGAGGCCTCG AAGGCAGGGC TCAGGATTTA CCGGCCCGGG 
GTGAAGGTGG AGGAGGAACA GTCACAGGGC TGGTTTGGCT GGATGTGGAA CTGGTCTGGA 
GAAGCCAGTG CCACTCAAAC CAAAGACGTC AAAACTGGAG GTTTTGATGG GTTAATGACG 
CCTGCAGAGA AAGCCAAACT ATACGCTGCC ATCGGATACA GCGAAACTGC TGTCAACCCC 
AACCTGCCAA AAAATTTTGA AAACATGAAG ATGAATTTCC ATCTTCTCCG CCTGTCCCTG 
TCCATCAAAG AGAGCCGAGA GAAGGCGGAG ATCATCAAAC TGTCTGTCAT TGACCTGAAG 
GCCATGCTGA CTCAAAGACC CGCGGCTCAA GCTATAAAGA TCTCCGCTCA GCTGTCGTCG 
TTTGAAGTGT CCGGTCTCCC GCGGGAAAAG TCGGCCCCGA TCCTGCTTTC TCCACGTCAT 
GTGGACGGCA TGAAGGATAA AAGTCTGCTG AACCTCATGT TCGAGACCAA CCCGCTGGAC 
GCTCGTGCTG ACCAGAGACT GCGCATCGAG TCCCAGCCGC TGGAGATCAT CTATGATGCA 
ATGACCGTGA ACAGTATGTC CGCTTTCTTC ATGCCTCCTA AAGACCTGCA GCTGGATGAA 
CTGACCAACG CCACACTGAT GAAGCTGGAG CAGTTCAGAG ACAAAACCTC CACAGGCCTC 
ATGTACATCA TTGAGACGCA GAAAGTTCTG GATCTGAAAA TAAACCTCAT GGCCTCGTAT 
GTCATCGTAC CGGAAAACGG CTTTTATGAC CCCAAACACA ACCTTGCCAT TCTAGATCTG 
GGTCATTTTC AGATGTCAAG TCAGAGCCGA GCTGATCTTC CTCAGCTGTC TGCGGGCAGC 
AGTACTCTAG AGGACATCAT GTCTCGAGCG TACGACAGCT TCGACGTCCA GCTCAGCAGT 
CTGCAGTTCC TCTACTCCAA AGCAGGTGGA GACTGGAAGC GGGCTCGTCC GCTAAGACAG 
TCTGCTTTCC ACATCCTGGA GCCAGTGGAC CTGAAGGTGG TCTTTAGCAG AGCGATGGTG 
GTCACAGACT CCAGGATGCC CAAGTTTAAG ATGCACGGTG AGCTGCCTCT GCTGTCTCTG 
CGGATCTCTG ATGATAAAGT GCGTGGCGTT CTGGCTCTGC TCAGCAGTAT TCCTCTACCT 
GAGAGCCGGC CCACGCCTCA GTCCAGAAAC ACGCAGGCCA CTCCAAAGGC CTCCAAGCGA 
GGAAATACCC CTCAGCTCAC GCCCCGAAGG CCAGCTATAG ATGACCTGCG TGCGTCCATC 
ATATACGGCT CAGAGGAGGA GCTGTTCTTT GACGCCCCCA GTTCACCGAT CGAGGATCTG 
CCGTTCTTCG AGGCCTCGTC GGACTCTCGG CATGCTTCAG CAGCTGATAA ACGTCTGTCT 
ATGGTGCTCA AAGACCCCAA GAAGAACATG ACTGAGTTTC TGCTTAGTTT TGAGATCAAC 
AAGCTCTCGG TGCAGCTGTG CCGTGTGTCG AATAGACAGG AAGTGAGTGT TCTGCATCTG 
TTCATCGATG GATTGGGAAC AGAGCTCAAA CTTCGCACGT TTGACATGTC GTCGTACACT 
TTCCTGCGTG AGATCTGCCT CGAGTGCTCA GAATACTTGG ATTCTGAGGA CAAGAAAGTT 
CACCTTATCA CCACGCTGGA CAACACAGAG GAGGATCTGC TCACTCTGGA GTATGTCAAG 
GCAGACAAAA ATGCACCAGA TTTTAAAGCT ATGTACAAAA ATACAGAGCA ACTAATCAAG 
GTGAATTTCT CCTCTCTGGA CATTCACCTG CACACAGAGG CTCTCCTGAA CTCCATGAAC 
TTCCTAAATA ATCTTCTGCC ACCGATGAAG AAGGAGACCC AGGAGGAACA ACCAATAATT 
CCCAAGGAGG AAGAGGAGGA GGAGGAGGAA GAAGAAGAAG AGGAGGAGGC TAAAGTAGAG 
AATTCTGCTT TTGAGGATGT GGTGAATTTG CACATCCGCG CAGACCTCAA CTGTCTGAAG 
GTGTTCATAC GAGGAGAGAA AGCCAGGATC TCTGAGATCA GCATAGAGGG ACTGGTTTCT 
GAAGTTCAGA TGAAGAAGAA GTCTGTGGAG ATTCTGGCTA ACTTGAAGAA CATCATCATT 
CTGGATTGTG ACAAAGAGGC ACTTTATAAA AAGGCAGTGT CCATCGCTGA TAAGGAGGTG 
TTTGATTTCC GCATGGTGAA CCATGTCGAT GCAACGGAGG GTGACGCTTA CACAGACATG 
CACATTGTGG ACACTAGCGT AACCCTGACC GTGGGCTGCA TTCAAGTTAT TTTCCTCAAC 
AAGTTTATGT CCTCAATACT GGCATTTATT AATAATTTCC AGGAGGCTAA AAATGCATTG 
GCGGAGGTGA CGGCCCAGGC AGCAGAGAAA GCAGCCACGG GGGTTCTGGA GCTGGCAGAG 
AGAAGTACTC GGATCTCCCT GGACGTCGAC ATCAAAGCTC CTGTTGTCTT CCTCCCACAA 
TCCTCAGTTT CCCACAACGT CATTGTGGCA GATCTGGGTT TAGTGACTGT AAAGAACCGC 
TTTGAGATCG TCTCCTCTCC AACACACACC AAGATCCCTC CTGTCATGGA CATCATGGCT 
GTCGGATTGA GTGATCTGAA AATGTACAGG ACAACGTTCA CTGATGACAA GTTTAACAGC 
GAGATTCAGT TGCTCAAGCC AGTCAACATG GACCTGTCAA TCAAACGCAA TCTCTCCGCC 
TCCTGGTATC ACAGCATACC CGACATCCAG ATCAATGCTC ACCTCAAGCC CATGAGCCTG 
ATCTTGAGTC AGGAGGATCT GACTGTGGTG CTGAAGACTC TGAGTGAGAA TCTGTCCGAG 
ACGTCTGATG CTCCTCCAGC TCCGTCCAGC TCTGATAAAG GAGATACGGT TTCTAACAAA 
GGCTCCCAGG GTTCTGGAGG CAACACCGTT GTGACCGCAG CAGTGGTGGA GGGTCAGAAA 
TCAGGCAAAC TGAAGAACAC CCTAAAACTG AACTTCAAGT TTGACTCCAT GACGCTGGTC 
CTCTACAGCC CGAACAGCGA GCAAGCGAAA TTGTCCGACT CTCGTGATGA GAGTTTGAAG 
TTGGCCGAGT TCACACTGGG CACCATCTCC ACCAGTGTGC ACATGTTCTC CGACAGCTCC 
ATGAAGGCCT CGCTCAAACT CACCAACTGT CTGCTGGATG ACAAGAGGCA GGGAGTGAAG 
ATGATTACAT CCAGGATGAT GGCGATGCGT CAGGACCGAG AGGACAGTGT GATGGTGGAC 
GTCAGCTACC GCCAGAGTCG GGACGGGACT CGTCTGGACA CCCTCGTGCA AGACCTTTAT 
CTTTGTGCCA GTATGGAGTT TCTGCTCACT GTAGCTGATG TGTTTCTTAA AGCTCTGTCT 
GACGGCTTCT CCGGTCCTCC AAAGATTGCT CTGTGGCTGC TGAAATGCTT GGCCCCCGAT 
AAATTGGTTG CTAAAACCGA GATGAATGTG GTGGTGAAGA ACCCAGAGAT CGTGTTTGTG 
GCGGATCTGA CGCGTGCTGA AGCTCCGGCT CTGGTCATGA CCACTCAGTG TGAGCTGGTG 
ATGAAGAGCG AGCCCAGCGG TCAGTGCATG ACGGCCGCCA TTTCAGACCT GAAGATTGTG 
GCCTGTCCGT TCCTGCGGGA GAAGAGGGAG AACAACGTGA CGACGGTCCT ACAGCCCTGC 
GAGGTTTTCT TCCAGAGCCG ACAGTCAAAC ACTGAGCCGC AGTGCATCGA GCTGTCCATC 
AGCGCACTCA CGCTCAAGGT GTCTCCGATC ATCATAAACA CGGTCATCAC CATTCAGACG 
GCTCTAAGTC CTGCCGCTGA GACGCCGCAG GAGCTGGAGA GCCCCATCCC ACAGGACCTA 
TGGGAGCGCC GCAGCCTTCG CGACCTGAAG CTCTGGTTCC TGGAGGAGGA GGGCGAGGAG 
GTCAGCACGT CCGTCACGCG TCTCATTCCT CAGGGAGAGT CTCTGAAGAT GAGCATCGCC 
TCCATCTGCC TGACGCTGGA GGCCGGAGTG GGACACCGCA CCGTCCCCAT GCTGCTGGCC 
AAAGCCTCGT TCCAGGGGGA CGTCAAGGAC TGGAGCACTC TCATCAACCT GCACTGCCAC 
CTCAGCATGG AGGTGAACTA CTATAATGAG ATGATGGGTT TATGGGAGCC GCTGTTGGAG 
CCGCTGGACG ATGAGGATAA AGACGGTTTT AGACCCTGGA CTCTCGCACT GAAGATGAAG 
AAGAAGCCGG TGGTCAGTTC TGGGGTGTCT GATGAGGTGG ACGGCGCTGT GCCTGATTAT 
AAAACGGTCA TCAGCTTCAC TAGTAAAGAC CAGCTTAACG TCACCCTGTC CAAGTGTGGT 
CTGCACATGC TCAGTAATCT GGGAACGGCT TTTGCAGAGG CCGCTAAACA GACGGCCGAG 
TGCTTTCAGA CAGATCAGAC ACCATTCGTG GTGAAGAATC GCCTGGGTTT GGCGGTGTCT 
GTGCTGCACA GCGAAGTGTT TCGACCCATC GGGAAGGAGT CTAAGGATCG TGTGGTGGAG 
CTCCCGGATG GAGAGAGTCT GAATTTGGAC TATACCCTCA CCGCCGAGTC CAGCGACCAG 
TTCTCTGCCA TGACCAGCCT CAGTGCCAAA AACTACATAC AGCCCACTCC AGTCGGCCAC 
ACGACCGCAA GTGTGATCCC GCTCATCAAA ACGGGCCGGG GCATGTACAG CGTCATGCAC 
AACGAGTCCC GCGTCACTCG CTTCTTCGTC TGTCAGATTT ACACCGTCGA GGGCAGCAAA 
TATGTCAAGA TTCGCTCTCC CTTCCAGATT ATCAACCACT TTTCAGTGCC GTTCCGTGTT 
TATGAAGGAT CCGTGTTTCT GGGCGAGTCT CTGCCGACAG AGGAGTTCTG TGTGCCGCTG 
GGATCCTACA GGTCTCTTAC AGCATTTCTG CAGCCTGTGC CTGATGGCGG TGAAGAGTAC 
GAGCACTCCG AGAGCTTCAC CTACACTGAG GTTGTCCAGA AACCCGGAAA GTGTCTGGAG 
AAAAACTGCC ATCTGCGTGG TGACAGCGCC CAGATTCTGA AGGTCAACAT GGTTCCTCTT 
GCGGACACAG TGACGTCCCA CAGCAGCGAG GAGGAGTTCG ATAAGCCCAT CGTACTGCAT 
TTGTGGCCCA CCGTCCTGCT GCGCAACCTG CTGCCCTACC CAATTACTTT CAAGCTAAAG 
GGCATCGGAG ATTCCCCCTC GGTGTCGCTG AAGGAGGGCC ACTCATCCCA GATCCACACC 
GCCATCATGG ACTTCTCCGT GTTGGATCTG AGGCTGCTGG GATACTTGCA GCAGGACTGG 
GAGTCTCAGT ACAGCCTCAG CAGTGAACAA GACGAGATCA GCTTCATTAA GTTTCGCTCG 
CTCGCACGTG AGGAGGAGAC TGAGAGCCGG CCGGAGCTGG ACATCGCTAT GCATGTGAAG 
AAGGAGGAGG GTCAGGTTGT GCTGGCCGTA CACTCGCCGT ACTGGATGGT GAATAAAACA 
AGCCGACTGC TGCAGTACAA GGCGGATGAT ATTCATCGCA AACACCCCAA GGATTACGAC 
ATGCCGCTGC TCTTCTCCTT CAAGCCACGC AACTTCCTGC AAAACAACAA GATCCGCCTG 
ATGATCTCAG AAAGTGAGCT GTCAGATGAT TTCTCCATCG ACACTGTCGG GAGTTACGGA 
GATGTCAAAT GCAAAGGCAG ACAGAAGGAT TACTTGGTGG GAGTGAAGAT CGATGCTAGC 
AGCTTCAGCT TGACACGTTT GGTGACTTTT CTGCCATTCT ACATGCTGGT GAACAGAACC 
AAACATGTGA TTCGGGTCTG TGAGGAGGGG CAGGAGAACT GGTTAAGTTC TTCTGTTCAG 
GCTGCTGTTC CATTCTGGCC AGAAAGAGAC TCAAAGAAGC TGAGAGTGAA GGTGGAGGGC 
AGTCAGTCGT CACCACGCGC CTTTGACTTC CACCAGCCAG AGAACTGCCT CCTGCTGCAT 
CTGGACAAAA GCTTGGGTGG CATCATTGTG GATGTGAATC TGACAGAGCA TTCAGCTGTG 
ATCCTCTTCT CTGATTATCA TGATGGAGCC GCTCCCTTCC TCCTCATCAA CCACACTAAG 
GACGAGACGC TGCAGTACCA CCAGAGCTCT CAGGCCGAGA TCGATGAACT GAGTCCAGGT 
CAGGCGGTTT ACTACACCTG GGCTGAACCA ACCGGCTCCA GGATCCTCAG CTGGAAACTG 
GGCAAATACA AAGGAGAGCT CAAGAGTGAA GAGGATCAGT TGCTGGATGT GGACAAGGAG 
GGCAAGTTGT TTTTGGTGTC GTTTTATGAA GGCCTCCAGC GTGTGGCGCT CTTCACCAGC 
GAGCAGCGCA TCTTCAAGAA GATCTGCGAC AGTGAGAAGA TAGAGCTGGC TCAGCAAGAA 
ATCAGCATCT TGCTGCAGAA CATGGGCATT TCGCTGGTCA ACAACAACTC CAGCCAGGAA 
GTGGCCTTCA TTGGCATCAC CAGCTCTGAT GTTGTGTGGG AAACCAAGCC GAAGAAGCGC 
AGCCGCTGGA AAGTTCTGAG TGTTAAAGAG GCAGCAGTTC TGGAGCAGCA CTACAGAGAC 
TACATGGAGA ACGGCCCAGT GGACAGCACC ATCATAGACC TGGACAACGA CTACCAGGTG 
TTGTTTGCTG CTAACGGTGT GGACATGAGG ATCATGCAGC CCTACGATGC TCCACTCAGA 
AGGAACTTCC TGCCGGCGTT GAAGGTGGAG TACAGCGTTT CAGCCCGCCA GAAAACATAC 
AGAGTCCAGA TCAACAGGAT CCAGATCCAA AACCAGCTGC AAGGAGCCAT ATTCCCGTTT 
GTGTTTTACC CCATCAAACC GCCAAAGTCT GTCAGCATGG ACTCAGAGCC AAAACCGCTG 
GCTGACGTGA GCATCATCAC CAGAGCAGCA GGACACTCCG ACATTCTGCG CATCAAATAT 
TTTATGGTGT TGATTCAGGA GATGGACCTG CGGCTGGATC TGGGCTTTCT GTATGCCATA 
CTTGAGCTCT TCACTGCTGA ACACACCAGT GCTGTGACGT CTGAACAGGA GGTGGAGTTG 
TTTGATAAAG ACATAGAGTA TCTAAAGACT GAGTTAAATC ATGTTTCAGC TACAGACACA 
TCTCCCATCA GCCTCTACGA GTACTTCCAC ATCTCTCCTA TAAAGCTTCA CTTGAGCTTG 
TCTCTGAGCA GTGGTGGTGA AGACAGCAAT AAGCAAGAGC GCGAGACGGA GCTGATCCCA 
GTTCAGTCTC TCCATCTGCT GCTCAAGAGC ATCGGGGCTA CACTTACAGA CGCACAGGAC 
GTCCTCTTTA AACTGGGCTT CTTCGAGCTG AGGTATCAGT TTCGCACCAC TCAGCAGCTG 
CAGTGGGAGG TCGTTCGCCA TTACTCCAAA CAGGCTATCA AGCAGATGTA TGTCCTTGTT 
CTGGGTTTGG ACGTTCTTGG GAATCCATTC GGGCTGATCC GGGGCCTGTC TGAAGGCGTC 
GAGGCTTTCT TTTACGAGCC CTATCAGGGT GCAATTCAGG GTCCAGAGGA GTTCATGGAG 
GGCATGGCTC TCGGCGTGAA GGCTCTGGTT GGAGGCGCTG TGGGTGGTCT GGCCGGAGCG 
GCGTCTCGCA TCACTGGAGC GATGGCTAAA GGTGTGGCGG CGATGACGAT GGATGAAGAG 
TACCAGCAGA AGAGACGAGA GGCCATGAAC AAACAGCCCA GCGGACTGAG AGAAGGACTG 
ACACGAGGAG GAAAAGGACT GGTGTCTGGC TTCGTGAGTG GCATCACGGG CATTGTGACC 
AAACCCATTA AAGGAGCTCA GAAGGAAGGA GCGGCAGGTT TCTTCAAGGG TATGGGTAAG 
GGTTTAGTTG GAGCTCTGAC CAGACCAACA GGGGGCATCA TTGACATGGC CAGCAGCACC 
TTCCAGGGCA TCAAGAGAGC TGCAGAGCTG TCTCAGGATG TGGAGAGTCT GCGCCCTCCA 
CGCTTCATCC ACGAGGACGG AGTGATCCGG CCGTACAGAG AGAGAGAAGG CCTCGGCAGT 
CAGATGCTGC AGAGTATTGA AAACGGACGG TTTGCCAAGT ACAGGTACTT TGCTCACGCT 
AAAGTCAACG AGTCTGATTT CCTGATGATC ACTAAAAGGG GGATCTTTTT CGTGACAAAG 
GGCACGTTTG GTCAGTTGAC ATGTGAATGG CAGTATCTGT TCGATGAGTT TACTAAAGCA 
CCCATGATTG TGGAAGGCAG GAGACTGCGC ATCGAAGCGA AGGAGAGAGT CAAGTCAGTG 
TTTCACGCCA AAGAGTTCGG GAAGATCATC AATTTCAAAA CTCCAGAATT AGCAAAGTGG 
GTTCTTGCCA AGCTTGAAGA CGCTCGGGAG AATCTGCCCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS13A ( XM_005167334.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005167391.1 Danio rerio vacuolar protein sorting 13 homolog A (S. cerevisiae) (vps13a), transcript variant X1, mRNA.

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CloneID	ODa14675
Clone ID Related Accession (Same CDS sequence)	XM_005167334.4 , XM_005167334.3
Accession Version	XM_005167334.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	vacuolar protein sorting-associated protein 13A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005167334.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGTTTG AGTCGGTGGT GGTCGACGTG CTGAACCGGT TTCTGGGGGA CTATGTTGGA 
AACCTCGACA GCTCGCAGCT CAGCCTCGGC ATATGGGGAG GTGATGCCAT ATTGAGGAAC 
CTCGAGATTA AAGAAAATGC ATTGAGCCAG CTGGACATTC CTTTCAAAGT CAGAGCAGGA 
CACATTGGGC GTTTGGAGTT AAAGATCCCC TGGAAGAACC TGTACACTCA GTCTGTGGAG 
GCCACGCTGG ATGAAGTTTA CCTGCTCATC GTACCTACTG CTAGTATAAA GTATGACGCA 
GAGAAGGAGG AGCGGCAGCT GCAGGAGTCT CGGCAGAGGG AGCTCCAGCG CATCGAGGAG 
ACCAAACAGA AAGCAGCAGA AAAAGAGAAT CCAAAGGGTG AGAAGCAGGA CACATTTGTG 
GAGAAACTGG TAACACAGGT GATCAAAAAC CTGCAGGTCA AAATATCCAA CATTCACGTC 
CGCTATGAGG ATGATGTCAC CAATCCCAGT GTTCCTCTCT CCTTTGGGAT ATCATTACAA 
AACCTCAGTC TCCAGACGGC GGATCAGAAC TGGAACCCCT GTCTTCTGGA TGAGAAAGCC 
AAACTGTTTT TTAAGCTTGT ACGTCTGGAT AATCTGTTTG CGTACTGGAA CGTCAGTTCA 
GAGCTGTACT CCAACCACAA CCCTGAGGAG GCTCTGCGAT ACCTGAAGCA CAGCGTAGCC 
ATCCAGACTG CCATCCCTCA GGATTATCAC TTCATCTTTC GTCCAATATC TGCCAATGCC 
AAGTTGCGGA TGAACCCTCG CTCTGATGTA GACTTCTCCT CACCCAAAAT GGACCTGGTG 
GTCAACCTTC CTGAAGTGGC CGTGGAGCTC AGCCGACCAC AGTACGTCAG CATTCTGGAG 
CTGTTGGGGT CGGTGGATAT GATGACCCGT AACCTTCCTT ACAGGAAGTA TCGGCCATAT 
GTGGTGGTTC ACACTAACGC TCGTCAGTGG TGGCGATATG TGATCACGGG TGTTCTGGAG 
GTCAATGTGA AGCCTCGTCT TCACATGTGG TCTTGGCTGC ATATTCGTCG ACATCGGCAG 
CATGTGAAAA AGTACAGAGA GCTTTATAAG AATAAAATCA TCAGTAAGAA ACCGGCAGAG 
AGTCTGCTGA AGGAGCTGGA GGAGACTGAA AGAACATTAG ACATATTCAA CATCACAATG 
GCGAGACAGC AGGCTGAGAT GGAGGCCTCG AAGGCAGGGC TCAGGATTTA CCGGCCCGGG 
GTGAAGGTGG AGGAGGAACA GTCACAGGGC TGGTTTGGCT GGATGTGGAA CTGGTCTGGA 
GAAGCCAGTG CCACTCAAAC CAAAGACGTC AAAACTGGAG GTTTTGATGG GTTAATGACG 
CCTGCAGAGA AAGCCAAACT ATACGCTGCC ATCGGATACA GCGAAACTGC TGTCAACCCC 
AACCTGCCAA AAAATTTTGA AAACATGAAG ATGAATTTCC ATCTTCTCCG CCTGTCCCTG 
TCCATCAAAG AGAGCCGAGA GAAGGCGGAG ATCATCAAAC TGTCTGTCAT TGACCTGAAG 
GCCATGCTGA CTCAAAGACC CGCGGCTCAA GCTATAAAGA TCTCCGCTCA GCTGTCGTCG 
TTTGAAGTGT CCGGTCTCCC GCGGGAAAAG TCGGCCCCGA TCCTGCTTTC TCCACGTCAT 
GTGGACGGCA TGAAGGATAA AAGTCTGCTG AACCTCATGT TCGAGACCAA CCCGCTGGAC 
GCTCGTGCTG ACCAGAGACT GCGCATCGAG TCCCAGCCGC TGGAGATCAT CTATGATGCA 
ATGACCGTGA ACAGTATGTC CGCTTTCTTC ATGCCTCCTA AAGACCTGCA GCTGGATGAA 
CTGACCAACG CCACACTGAT GAAGCTGGAG CAGTTCAGAG ACAAAACCTC CACAGGCCTC 
ATGTACATCA TTGAGACGCA GAAAGTTCTG GATCTGAAAA TAAACCTCAT GGCCTCGTAT 
GTCATCGTAC CGGAAAACGG CTTTTATGAC CCCAAACACA ACCTTGCCAT TCTAGATCTG 
GGTCATTTTC AGATGTCAAG TCAGAGCCGA GCTGATCTTC CTCAGCTGTC TGCGGGCAGC 
AGTACTCTAG AGGACATCAT GTCTCGAGCG TACGACAGCT TCGACGTCCA GCTCAGCAGT 
CTGCAGTTCC TCTACTCCAA AGCAGGTGGA GACTGGAAGC GGGCTCGTCC GCTAAGACAG 
TCTGCTTTCC ACATCCTGGA GCCAGTGGAC CTGAAGGTGG TCTTTAGCAG AGCGATGGTG 
GTCACAGACT CCAGGATGCC CAAGTTTAAG ATGCACGGTG AGCTGCCTCT GCTGTCTCTG 
CGGATCTCTG ATGATAAAGT GCGTGGCGTT CTGGCTCTGC TCAGCAGTAT TCCTCTACCT 
GAGAGCCGGC CCACGCCTCA GTCCAGAAAC ACGCAGGCCA CTCCAAAGGC CTCCAAGCGA 
GGAAATACCC CTCAGCTCAC GCCCCGAAGG CCAGCTATAG ATGACCTGCG TGCGTCCATC 
ATATACGGCT CAGAGGAGGA GCTGTTCTTT GACGCCCCCA GTTCACCGAT CGAGGATCTG 
CCGTTCTTCG AGGCCTCGTC GGACTCTCGG CATGCTTCAG CAGCTGATAA ACGTCTGTCT 
ATGGTGCTCA AAGACCCCAA GAAGAACATG ACTGAGTTTC TGCTTAGTTT TGAGATCAAC 
AAGCTCTCGG TGCAGCTGTG CCGTGTGTCG AATAGACAGG AAGTGAGTGT TCTGCATCTG 
TTCATCGATG GATTGGGAAC AGAGCTCAAA CTTCGCACGT TTGACATGTC GTCGTACACT 
TTCCTGCGTG AGATCTGCCT CGAGTGCTCA GAATACTTGG ATTCTGAGGA CAAGAAAGTT 
CACCTTATCA CCACGCTGGA CAACACAGAG GAGGATCTGC TCACTCTGGA GTATGTCAAG 
GCAGACAAAA ATGCACCAGA TTTTAAAGCT ATGTACAAAA ATACAGAGCA ACTAATCAAG 
GTGAATTTCT CCTCTCTGGA CATTCACCTG CACACAGAGG CTCTCCTGAA CTCCATGAAC 
TTCCTAAATA ATCTTCTGCC ACCGATGAAG AAGGAGACCC AGGAGGAACA ACCAATAATT 
CCCAAGGAGG AAGAGGAGGA GGAGGAGGAA GAAGAAGAAG AGGAGGAGGC TAAAGTAGAG 
AATTCTGCTG TGGCCAAGAA GTCTTCCTAC AAGAAGTCAA AGTTTGAGGA TGTGGTGAAT 
TTGCACATCC GCGCAGACCT CAACTGTCTG AAGGTGTTCA TACGAGGAGA GAAAGCCAGG 
ATCTCTGAGA TCAGCATAGA GGGACTGGTT TCTGAAGTTC AGATGAAGAA GAAGTCTGTG 
GAGATTCTGG CTAACTTGAA GAACATCATC ATTCTGGATT GTGACAAAGA GGCACTTTAT 
AAAAAGGCAG TGTCCATCGC TGATAAGGAG GTGTTTGATT TCCGCATGGT GAACCATGTC 
GATGCAACGG AGGGTGACGC TTACACAGAC ATGCACATTG TGGACACTAG CGTAACCCTG 
ACCGTGGGCT GCATTCAAGT TATTTTCCTC AACAAGTTTA TGTCCTCAAT ACTGGCATTT 
ATTAATAATT TCCAGGAGGC TAAAAATGCA TTGGCGGAGG TGACGGCCCA GGCAGCAGAG 
AAAGCAGCCA CGGGGGTTCT GGAGCTGGCA GAGAGAAGTA CTCGGATCTC CCTGGACGTC 
GACATCAAAG CTCCTGTTGT CTTCCTCCCA CAATCCTCAG TTTCCCACAA CGTCATTGTG 
GCAGATCTGG GTTTAGTGAC TGTAAAGAAC CGCTTTGAGA TCGTCTCCTC TCCAACACAC 
ACCAAGATCC CTCCTGTCAT GGACATCATG GCTGTCGGAT TGAGTGATCT GAAAATGTAC 
AGGACAACGT TCACTGATGA CAAGTTTAAC AGCGAGATTC AGTTGCTCAA GCCAGTCAAC 
ATGGACCTGT CAATCAAACG CAATCTCTCC GCCTCCTGGT ATCACAGCAT ACCCGACATC 
CAGATCAATG CTCACCTCAA GCCCATGAGC CTGATCTTGA GTCAGGAGGA TCTGACTGTG 
GTGCTGAAGA CTCTGAGTGA GAATCTGTCC GAGACGTCTG ATGCTCCTCC AGCTCCGTCC 
AGCTCTGATA AAGGAGATAC GGTTTCTAAC AAAGGCTCCC AGGGTTCTGG AGGCAACACC 
GTTGTGACCG CAGCAGTGGT GGAGGGTCAG AAATCAGGCA AACTGAAGAA CACCCTAAAA 
CTGAACTTCA AGTTTGACTC CATGACGCTG GTCCTCTACA GCCCGAACAG CGAGATCCAG 
TTGTCCGACT CTCGTGATGA GAGTTTGAAG TTGGCCGAGT TCACACTGGG CACCATCTCC 
ACCAGTGTGC ACATGTTCTC CGACAGCTCC ATGAAGGCCT CGCTCAAACT CACCAACTGT 
CTGCTGGATG ACAAGAGGCA GGGAGTGAAG ATGATTACAT CCAGGATGAT GGCGATGCGT 
CAGGACCGAG AGGACAGTGT GATGGTGGAC GTCAGCTACC GCCAGAGTCG GGACGGGACT 
CGTCTGGACA CCCTCGTGCA AGACCTTTAT CTTTGTGCCA GTATGGAGTT TCTGCTCACT 
GTAGCTGATG TGTTTCTTAA AGCTCTGTCT GACGGCTTCT CCGGTCCTCC AAAGAGTAAC 
AAACAACCGG CCAGCACCTC TAAAGACCCA GCGGCTCCTG CTCCACCAGT GGTTGCTAAA 
ACCGAGATGA ATGTGGTGGT GAAGAACCCA GAGATCGTGT TTGTGGCGGA TCTGACGCGT 
GCTGAAGCTC CGGCTCTGGT CATGACCACT CAGTGTGAGC TGGTGATGAA GAGCGAGCCC 
AGCGGTCAGT GCATGACGGC CGCCATTTCA GACCTGAAGA TTGTGGCCTG TCCGTTCCTG 
CGGGAGAAGA GGGAGAACAA CGTGACGACG GTCCTACAGC CCTGCGAGGT TTTCTTCCAG 
AGCCGACAGT CAAACACTGA GCCGCAGTGC ATCGAGCTGT CCATCAGCGC ACTCACGCTC 
AAGGTGTCTC CGATCATCAT AAACACGGTC ATCACCATTC AGACGGCTCT AAGTCCTGCC 
GCTGAGACGC CGCAGGAGCT GGAGAGCCCC ATCCCACAGG ACCTATGGGA GCGCCGCAGC 
CTTCGCGACC TGAAGCTCTG GTTCCTGGAG GAGGAGGGCG AGGAGGTCAG CACGTCCGTC 
ACGCGTCTCA TTCCTCAGGG AGAGTCTCTG AAGATGAGCA TCGCCTCCAT CTGCCTGACG 
CTGGAGGCCG GAGTGGGACA CCGCACCGTC CCCATGCTGC TGGCCAAAGC CTCGTTCCAG 
GGGGACGTCA AGGACTGGAG CACTCTCATC AACCTGCACT GCCACCTCAG CATGGAGGTG 
AACTACTATA ATGAGATGAT GGGTTTATGG GAGCCGCTGT TGGAGCCGCT GGACGATGAG 
GATAAAGACG GTTTTAGACC CTGGACTCTC GCACTGAAGA TGAAGAAGAA GCCGGTGGTC 
AGTTCTGGGG TGTCTGATGA GGTGGACGGC GCTGTGCCTG ATTATAAAAC GGTCATCAGC 
TTCACTAGTA AAGACCAGCT TAACGTCACC CTGTCCAAGT GTGGTCTGCA CATGCTCAGT 
AATCTGGGAA CGGCTTTTGC AGAGGCCGCT AAACAGACGG CCGAGTGCTT TCAGACAGAT 
CAGACACCAT TCGTGGTGAA GAATCGCCTG GGTTTGGCGG TGTCTGTGCT GCACAGCGAA 
GTGTTTCGAC CCATCGGGAA GGAGTCTAAG GATCGTGTGG TGGAGCTCCC GGATGGAGAG 
AGTCTGAATT TGGACTATAC CCTCACCGCC GAGTCCAGCG ACCAGTTCTC TGCCATGACC 
AGCCTCAGTG CCAAAAACTA CATACAGCCC ACTCCAGTCG GCCACACGAC CGCAAGTGTG 
ATCCCGCTCA TCAAAACGGG CCGGGGCATG TACAGCGTCA TGCACAACGA GTCCCGCGTC 
ACTCGCTTCT TCGTCTGTCA GATTTACACC GTCGAGGGCA GCAAATATGT CAAGATTCGC 
TCTCCCTTCC AGATTATCAA CCACTTTTCA GTGCCGTTCC GTGTTTATGA AGGATCCGTG 
TTTCTGGGCG AGTCTCTGCC GACAGAGGAG TTCTGTGTGC CGCTGGGATC CTACAGAGCT 
GAGCTGAGTC TGCAGCCTGT GCCTGATGGC GGTGAAGAGT ACGAGCACTC CGAGAGCTTC 
ACCTACACTG AGGTTGTCCA GAAACCCGGA AAGTGTCTGG AGAAAAACTG CCATCTGCGT 
GGTGACAGCG CCCAGATTCT GAAGGTCAAC ATGGTTCCTC TTGCGGACAC AGTGACGTCC 
CACAGCAGCG AGGAGGAGTT CGATAAGCCC ATCGTACTGC ATTTGTGGCC CACCGTCCTG 
CTGCGCAACC TGCTGCCCTA CCCAATTACT TTCAAGCTAA AGGGCATCGG AGATTCCCCC 
TCGGTGTCGC TGAAGGAGGG CCACTCATCC CAGATCCACA CCGCCATCAT GGACTTCTCC 
GTGTTGGATC TGAGGCTGCT GGGATACTTG CAGCAGGACT GGGAGTCTCA GTACAGCCTC 
AGCAGTGAAC AAGACGAGAT CAGCTTCATT AAGTTTCGCT CGCTCGCACG TGAGGAGGAG 
ACTGAGAGCC GGCCGGAGCT GGACATCGCT ATGCATGTGA AGAAGGAGGA GGGTCAGGTT 
GTGCTGGCCG TACACTCGCC GTACTGGATG GTGAATAAAA CAAGCCGACT GCTGCAGTAC 
AAGGCGGATG ATATTCATCG CAAACACCCC AAGGATTACG ACATGCCGCT GCTCTTCTCC 
TTCAAGCCAC GCAACTTCCT GCAAAACAAC AAGATCCGCC TGATGATCTC AGAAAGTGAG 
CTGTCAGATG ATTTCTCCAT CGACACTGTC GGGAGTTACG GAGATGTCAA ATGCAAAGGC 
AGACAGAAGG ATTACTTGGT GGGAGTGAAG ATCGATGCTA GCAGCTTCAG CTTGACACGT 
TTGGTGACTT TTCTGCCATT CTACATGCTG GTGAACAGAA CCAAACATGT GATTCGGGTC 
TGTGAGGAGG GGCAGGAGAA CTGGACAGAA GCCCGACCCG GACAGGCTGC TGTTCCATTC 
TGGCCAGAAA GAGACTCAAA GAAGCTGAGA GTGAAGGTGG AGGGCAGTCA GTCGTCACCA 
CGCGCCTTTG ACTTCCACCA GCCAGAGAAC TGCCTCCTGC TGCATCTGGA CAAAAGCTTG 
GGTGGCATCA TTGTGGATGT GAATCTGACA GAGCATTCAG CTGTGATCCT CTTCTCTGAT 
TATCATGATG GAGCCGCTCC CTTCCTCCTC ATCAACCACA CTAAGGACGA GACGCTGCAG 
TACCACCAGA GCAACAGTAA GAGGAAGCCT TCGACGTGGT TTCAGGATGA AATACTGGAT 
TTATCAATGA GGGATGAGAC TGATTCCCTC TGCTCTCAGG CCGAGATCGA TGAACTGAGT 
CCAGGTCAGG CGGTTTACTA CACCTGGGCT GAACCAACCG GCTCCAGGAT CCTCAGCTGG 
AAACTGGGCA AATACAAAGG AGAGCTCAAG AGTGAAGAGG ATCAGTTGCT GGATGTGGAC 
AAGGAGGGCA AGTTGTTTTT GGTGTCGTTT TATGAAGGCC TCCAGCGTGT GGCGCTCTTC 
ACCAGCGAGC AGCGCATCTT CAAGAAGATC TGCGACAGTG AGAAGATAGA GCTGGCTCAG 
CAAGAAATCA GCATCTTGCT GCAGAACATG GGCATTTCGC TGGTCAACAA CAACTCCAGC 
CAGGAAGTGG CCTTCATTGG CATCACCAGC TCTGATGTTG TGTGGGAAAC CAAGCCGAAG 
AAGCGCAGCC GCTGGAAAGT TCTGAGTGTT AAAGAGGCAG CAGTTCTGGA GCAGCACTAC 
AGAGACTACA TGGAGAACGG CCCAGTGGAC AGCACCATCA TAGACCTGGA CAACGACTAC 
CAGGTGTTGT TTGCTGCTAA CGGTGTGGAC ATGAGGATCA TGCAGCCCTA CGATGCTCCA 
CTCAGAAGGA ACTTCCTGCC GGCGTTGAAG GTGGAGTACA GCGTTTCAGC CCGCCAGAAA 
ACATACAGAG TCCAGATCAA CAGGATCCAG ATCCAAAACC AGCTGCAAGG AGCCATATTC 
CCGTTTGTGT TTTACCCCAT CAAACCGCCA AAGTCTGTCA GCATGGACTC AGAGCCAAAA 
CCGCTGGCTG ACGTGAGCAT CATCACCAGA GCAGCAGGAC ACTCCGACAT TCTGCGCATC 
AAATATTTTA TGGTGTTGAT TCAGGAGATG GACCTGCGGC TGGATCTGGG CTTTCTGTAT 
GCCATACTTG AGCTCTTCAC TGCTGAACAC ACCAGTGCTG TGACGTCTGA ACAGGAGGTG 
GAGTTGTTTG ATAAAGACAT AGAGTATCTA AAGACTGAGT TAAATCATGT TTCAGCTACA 
GACACATCTC CCATCAGCCT CTACGAGTAC TTCCACATCT CTCCTATAAA GCTTCACTTG 
AGCTTGTCTC TGAGCAGTGG TGGTGAAGAC AGCAATAAGC AAGAGCGCGA GACGGAGCTG 
ATCCCAGTTC AGTCTCTCCA TCTGCTGCTC AAGAGCATCG GGGCTACACT TACAGACGCA 
CAGGACGTCC TCTTTAAACT GGGCTTCTTC GAGCTGAGGT ATCAGTTTCG CACCACTCAG 
CAGCTGCAGT GGGAGGTCGT TCGCCATTAC TCCAAACAGG CTATCAAGCA GATGTATGTC 
CTTGTTCTGG GTTTGGACGT TCTTGGGAAT CCATTCGGGC TGATCCGGGG CCTGTCTGAA 
GGCGTCGAGG CTTTCTTTTA CGAGCCCTAT CAGGGTGCAA TTCAGGGTCC AGAGGAGTTC 
ATGGAGGGCA TGGCTCTCGG CGTGAAGGCT CTGGTTGGAG GCGCTGTGGG TGGTCTGGCC 
GGAGCGGCGT CTCGCATCAC TGGAGCGATG GCTAAAGGTG TGGCGGCGAT GACGATGGAT 
GAAGAGTACC AGCAGAAGAG ACGAGAGGCC ATGAACAAAC AGCCCAGCGG ACTGAGAGAA 
GGACTGACAC GAGGAGGAAA AGGACTGGTG TCTGGCTTCG TGAGTGGCAT CACGGGCATT 
GTGACCAAAC CCATTAAAGG AGCTCAGAAG GAAGGAGCGG CAGGTTTCTT CAAGGGTATG 
GGTAAGGGTT TAGTTGGAGC TCTGACCAGA CCAACAGGGG GCATCATTGA CATGGCCAGC 
AGCACCTTCC AGGGCATCAA GAGAGCTGCA GAGCTGTCTC AGGATGTGGA GAGTCTGCGC 
CCTCCACGCT TCATCCACGA GGACGGAGTG ATCCGGCCGT ACAGAGAGAG AGAAGGCCTC 
GGCAGTCAGA TGCTGCAGAG TATTGAAAAC GGACGGTTTG CCAAGTACAG GTACTTTGCT 
CACGCTAAAG TCAACGAGTC TGATTTCCTG ATGATCACTA AAAGGGGGAT CTTTTTCGTG 
ACAAAGGGCA CGTTTGGTCA GTTGACATGT GAATGGCAGT ATCTGTTCGA TGAGTTTACT 
AAAGCACCCA TGATTGTGGA AGGCAGGAGA CTGCGCATCG AAGCGAAGGA GAGAGTCAAG 
TCAGTGTTTC ACGCCAAAGA GTTCGGGAAG ATCATCAATT TCAAAACTCC AGAATTAGCA 
AAGTGGGTTC TTGCCAAGCT TGAAGACGCT CGGGAGAATC TGCCCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167391.1
CDS	153..9863
Translation	MVFESVVVDVLNRFLGDYVGNLDSSQLSLGIWGGDAILRNLEIKENALSQLDIPFKVRAGHIGRLELKIPWKNLYTQSVEATLDEVYLLIVPTASIKYDAEKEERQLQESRQRELQRIEETKQKAAEKENPKGEKQDTFVEKLVTQVIKNLQVKISNIHVRYEDDVTNPSVPLSFGISLQNLSLQTADQNWNPCLLDEKAKLFFKLVRLDNLFAYWNVSSELYSNHNPEEALRYLKHSVAIQTAIPQDYHFIFRPISANAKLRMNPRSDVDFSSPKMDLVVNLPEVAVELSRPQYVSILELLGSVDMMTRNLPYRKYRPYVVVHTNARQWWRYVITGVLEVNVKPRLHMWSWLHIRRHRQHVKKYRELYKNKIISKKPAESLLKELEETERTLDIFNITMARQQAEMEASKAGLRIYRPGVKVEEEQSQGWFGWMWNWSGEASATQTKDVKTGGFDGLMTPAEKAKLYAAIGYSETAVNPNLPKNFENMKMNFHLLRLSLSIKESREKAEIIKLSVIDLKAMLTQRPAAQAIKISAQLSSFEVSGLPREKSAPILLSPRHVDGMKDKSLLNLMFETNPLDARADQRLRIESQPLEIIYDAMTVNSMSAFFMPPKDLQLDELTNATLMKLEQFRDKTSTGLMYIIETQKVLDLKINLMASYVIVPENGFYDPKHNLAILDLGHFQMSSQSRADLPQLSAGSSTLEDIMSRAYDSFDVQLSSLQFLYSKAGGDWKRARPLRQSAFHILEPVDLKVVFSRAMVVTDSRMPKFKMHGELPLLSLRISDDKVRGVLALLSSIPLPESRPTPQSRNTQATPKASKRGNTPQLTPRRPAIDDLRASIIYGSEEELFFDAPSSPIEDLPFFEASSDSRHASAADKRLSMVLKDPKKNMTEFLLSFEINKLSVQLCRVSNRQEVSVLHLFIDGLGTELKLRTFDMSSYTFLREICLECSEYLDSEDKKVHLITTLDNTEEDLLTLEYVKADKNAPDFKAMYKNTEQLIKVNFSSLDIHLHTEALLNSMNFLNNLLPPMKKETQEEQPIIPKEEEEEEEEEEEEEEAKVENSAVAKKSSYKKSKFEDVVNLHIRADLNCLKVFIRGEKARISEISIEGLVSEVQMKKKSVEILANLKNIIILDCDKEALYKKAVSIADKEVFDFRMVNHVDATEGDAYTDMHIVDTSVTLTVGCIQVIFLNKFMSSILAFINNFQEAKNALAEVTAQAAEKAATGVLELAERSTRISLDVDIKAPVVFLPQSSVSHNVIVADLGLVTVKNRFEIVSSPTHTKIPPVMDIMAVGLSDLKMYRTTFTDDKFNSEIQLLKPVNMDLSIKRNLSASWYHSIPDIQINAHLKPMSLILSQEDLTVVLKTLSENLSETSDAPPAPSSSDKGDTVSNKGSQGSGGNTVVTAAVVEGQKSGKLKNTLKLNFKFDSMTLVLYSPNSEIQLSDSRDESLKLAEFTLGTISTSVHMFSDSSMKASLKLTNCLLDDKRQGVKMITSRMMAMRQDREDSVMVDVSYRQSRDGTRLDTLVQDLYLCASMEFLLTVADVFLKALSDGFSGPPKSNKQPASTSKDPAAPAPPVVAKTEMNVVVKNPEIVFVADLTRAEAPALVMTTQCELVMKSEPSGQCMTAAISDLKIVACPFLREKRENNVTTVLQPCEVFFQSRQSNTEPQCIELSISALTLKVSPIIINTVITIQTALSPAAETPQELESPIPQDLWERRSLRDLKLWFLEEEGEEVSTSVTRLIPQGESLKMSIASICLTLEAGVGHRTVPMLLAKASFQGDVKDWSTLINLHCHLSMEVNYYNEMMGLWEPLLEPLDDEDKDGFRPWTLALKMKKKPVVSSGVSDEVDGAVPDYKTVISFTSKDQLNVTLSKCGLHMLSNLGTAFAEAAKQTAECFQTDQTPFVVKNRLGLAVSVLHSEVFRPIGKESKDRVVELPDGESLNLDYTLTAESSDQFSAMTSLSAKNYIQPTPVGHTTASVIPLIKTGRGMYSVMHNESRVTRFFVCQIYTVEGSKYVKIRSPFQIINHFSVPFRVYEGSVFLGESLPTEEFCVPLGSYRAELSLQPVPDGGEEYEHSESFTYTEVVQKPGKCLEKNCHLRGDSAQILKVNMVPLADTVTSHSSEEEFDKPIVLHLWPTVLLRNLLPYPITFKLKGIGDSPSVSLKEGHSSQIHTAIMDFSVLDLRLLGYLQQDWESQYSLSSEQDEISFIKFRSLAREEETESRPELDIAMHVKKEEGQVVLAVHSPYWMVNKTSRLLQYKADDIHRKHPKDYDMPLLFSFKPRNFLQNNKIRLMISESELSDDFSIDTVGSYGDVKCKGRQKDYLVGVKIDASSFSLTRLVTFLPFYMLVNRTKHVIRVCEEGQENWTEARPGQAAVPFWPERDSKKLRVKVEGSQSSPRAFDFHQPENCLLLHLDKSLGGIIVDVNLTEHSAVILFSDYHDGAAPFLLINHTKDETLQYHQSNSKRKPSTWFQDEILDLSMRDETDSLCSQAEIDELSPGQAVYYTWAEPTGSRILSWKLGKYKGELKSEEDQLLDVDKEGKLFLVSFYEGLQRVALFTSEQRIFKKICDSEKIELAQQEISILLQNMGISLVNNNSSQEVAFIGITSSDVVWETKPKKRSRWKVLSVKEAAVLEQHYRDYMENGPVDSTIIDLDNDYQVLFAANGVDMRIMQPYDAPLRRNFLPALKVEYSVSARQKTYRVQINRIQIQNQLQGAIFPFVFYPIKPPKSVSMDSEPKPLADVSIITRAAGHSDILRIKYFMVLIQEMDLRLDLGFLYAILELFTAEHTSAVTSEQEVELFDKDIEYLKTELNHVSATDTSPISLYEYFHISPIKLHLSLSLSSGGEDSNKQERETELIPVQSLHLLLKSIGATLTDAQDVLFKLGFFELRYQFRTTQQLQWEVVRHYSKQAIKQMYVLVLGLDVLGNPFGLIRGLSEGVEAFFYEPYQGAIQGPEEFMEGMALGVKALVGGAVGGLAGAASRITGAMAKGVAAMTMDEEYQQKRREAMNKQPSGLREGLTRGGKGLVSGFVSGITGIVTKPIKGAQKEGAAGFFKGMGKGLVGALTRPTGGIIDMASSTFQGIKRAAELSQDVESLRPPRFIHEDGVIRPYREREGLGSQMLQSIENGRFAKYRYFAHAKVNESDFLMITKRGIFFVTKGTFGQLTCEWQYLFDEFTKAPMIVEGRRLRIEAKERVKSVFHAKEFGKIINFKTPELAKWVLAKLEDARENLPK

Target ORF information:

RefSeq Version	XM_005167334.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio vacuolar protein sorting 13 homolog A (S. cerevisiae) (vps13a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167334.4

ORF Insert Sequence:

ATGGTGTTTG AGTCGGTGGT GGTCGACGTG CTGAACCGGT TTCTGGGGGA CTATGTTGGA 
AACCTCGACA GCTCGCAGCT CAGCCTCGGC ATATGGGGAG GTGATGCCAT ATTGAGGAAC 
CTCGAGATTA AAGAAAATGC ATTGAGCCAG CTGGACATTC CTTTCAAAGT CAGAGCAGGA 
CACATTGGGC GTTTGGAGTT AAAGATCCCC TGGAAGAACC TGTACACTCA GTCTGTGGAG 
GCCACGCTGG ATGAAGTTTA CCTGCTCATC GTACCTACTG CTAGTATAAA GTATGACGCA 
GAGAAGGAGG AGCGGCAGCT GCAGGAGTCT CGGCAGAGGG AGCTCCAGCG CATCGAGGAG 
ACCAAACAGA AAGCAGCAGA AAAAGAGAAT CCAAAGGGTG AGAAGCAGGA CACATTTGTG 
GAGAAACTGG TAACACAGGT GATCAAAAAC CTGCAGGTCA AAATATCCAA CATTCACGTC 
CGCTATGAGG ATGATGTCAC CAATCCCAGT GTTCCTCTCT CCTTTGGGAT ATCATTACAA 
AACCTCAGTC TCCAGACGGC GGATCAGAAC TGGAACCCCT GTCTTCTGGA TGAGAAAGCC 
AAACTGTTTT TTAAGCTTGT ACGTCTGGAT AATCTGTTTG CGTACTGGAA CGTCAGTTCA 
GAGCTGTACT CCAACCACAA CCCTGAGGAG GCTCTGCGAT ACCTGAAGCA CAGCGTAGCC 
ATCCAGACTG CCATCCCTCA GGATTATCAC TTCATCTTTC GTCCAATATC TGCCAATGCC 
AAGTTGCGGA TGAACCCTCG CTCTGATGTA GACTTCTCCT CACCCAAAAT GGACCTGGTG 
GTCAACCTTC CTGAAGTGGC CGTGGAGCTC AGCCGACCAC AGTACGTCAG CATTCTGGAG 
CTGTTGGGGT CGGTGGATAT GATGACCCGT AACCTTCCTT ACAGGAAGTA TCGGCCATAT 
GTGGTGGTTC ACACTAACGC TCGTCAGTGG TGGCGATATG TGATCACGGG TGTTCTGGAG 
GTCAATGTGA AGCCTCGTCT TCACATGTGG TCTTGGCTGC ATATTCGTCG ACATCGGCAG 
CATGTGAAAA AGTACAGAGA GCTTTATAAG AATAAAATCA TCAGTAAGAA ACCGGCAGAG 
AGTCTGCTGA AGGAGCTGGA GGAGACTGAA AGAACATTAG ACATATTCAA CATCACAATG 
GCGAGACAGC AGGCTGAGAT GGAGGCCTCG AAGGCAGGGC TCAGGATTTA CCGGCCCGGG 
GTGAAGGTGG AGGAGGAACA GTCACAGGGC TGGTTTGGCT GGATGTGGAA CTGGTCTGGA 
GAAGCCAGTG CCACTCAAAC CAAAGACGTC AAAACTGGAG GTTTTGATGG GTTAATGACG 
CCTGCAGAGA AAGCCAAACT ATACGCTGCC ATCGGATACA GCGAAACTGC TGTCAACCCC 
AACCTGCCAA AAAATTTTGA AAACATGAAG ATGAATTTCC ATCTTCTCCG CCTGTCCCTG 
TCCATCAAAG AGAGCCGAGA GAAGGCGGAG ATCATCAAAC TGTCTGTCAT TGACCTGAAG 
GCCATGCTGA CTCAAAGACC CGCGGCTCAA GCTATAAAGA TCTCCGCTCA GCTGTCGTCG 
TTTGAAGTGT CCGGTCTCCC GCGGGAAAAG TCGGCCCCGA TCCTGCTTTC TCCACGTCAT 
GTGGACGGCA TGAAGGATAA AAGTCTGCTG AACCTCATGT TCGAGACCAA CCCGCTGGAC 
GCTCGTGCTG ACCAGAGACT GCGCATCGAG TCCCAGCCGC TGGAGATCAT CTATGATGCA 
ATGACCGTGA ACAGTATGTC CGCTTTCTTC ATGCCTCCTA AAGACCTGCA GCTGGATGAA 
CTGACCAACG CCACACTGAT GAAGCTGGAG CAGTTCAGAG ACAAAACCTC CACAGGCCTC 
ATGTACATCA TTGAGACGCA GAAAGTTCTG GATCTGAAAA TAAACCTCAT GGCCTCGTAT 
GTCATCGTAC CGGAAAACGG CTTTTATGAC CCCAAACACA ACCTTGCCAT TCTAGATCTG 
GGTCATTTTC AGATGTCAAG TCAGAGCCGA GCTGATCTTC CTCAGCTGTC TGCGGGCAGC 
AGTACTCTAG AGGACATCAT GTCTCGAGCG TACGACAGCT TCGACGTCCA GCTCAGCAGT 
CTGCAGTTCC TCTACTCCAA AGCAGGTGGA GACTGGAAGC GGGCTCGTCC GCTAAGACAG 
TCTGCTTTCC ACATCCTGGA GCCAGTGGAC CTGAAGGTGG TCTTTAGCAG AGCGATGGTG 
GTCACAGACT CCAGGATGCC CAAGTTTAAG ATGCACGGTG AGCTGCCTCT GCTGTCTCTG 
CGGATCTCTG ATGATAAAGT GCGTGGCGTT CTGGCTCTGC TCAGCAGTAT TCCTCTACCT 
GAGAGCCGGC CCACGCCTCA GTCCAGAAAC ACGCAGGCCA CTCCAAAGGC CTCCAAGCGA 
GGAAATACCC CTCAGCTCAC GCCCCGAAGG CCAGCTATAG ATGACCTGCG TGCGTCCATC 
ATATACGGCT CAGAGGAGGA GCTGTTCTTT GACGCCCCCA GTTCACCGAT CGAGGATCTG 
CCGTTCTTCG AGGCCTCGTC GGACTCTCGG CATGCTTCAG CAGCTGATAA ACGTCTGTCT 
ATGGTGCTCA AAGACCCCAA GAAGAACATG ACTGAGTTTC TGCTTAGTTT TGAGATCAAC 
AAGCTCTCGG TGCAGCTGTG CCGTGTGTCG AATAGACAGG AAGTGAGTGT TCTGCATCTG 
TTCATCGATG GATTGGGAAC AGAGCTCAAA CTTCGCACGT TTGACATGTC GTCGTACACT 
TTCCTGCGTG AGATCTGCCT CGAGTGCTCA GAATACTTGG ATTCTGAGGA CAAGAAAGTT 
CACCTTATCA CCACGCTGGA CAACACAGAG GAGGATCTGC TCACTCTGGA GTATGTCAAG 
GCAGACAAAA ATGCACCAGA TTTTAAAGCT ATGTACAAAA ATACAGAGCA ACTAATCAAG 
GTGAATTTCT CCTCTCTGGA CATTCACCTG CACACAGAGG CTCTCCTGAA CTCCATGAAC 
TTCCTAAATA ATCTTCTGCC ACCGATGAAG AAGGAGACCC AGGAGGAACA ACCAATAATT 
CCCAAGGAGG AAGAGGAGGA GGAGGAGGAA GAAGAAGAAG AGGAGGAGGC TAAAGTAGAG 
AATTCTGCTG TGGCCAAGAA GTCTTCCTAC AAGAAGTCAA AGTTTGAGGA TGTGGTGAAT 
TTGCACATCC GCGCAGACCT CAACTGTCTG AAGGTGTTCA TACGAGGAGA GAAAGCCAGG 
ATCTCTGAGA TCAGCATAGA GGGACTGGTT TCTGAAGTTC AGATGAAGAA GAAGTCTGTG 
GAGATTCTGG CTAACTTGAA GAACATCATC ATTCTGGATT GTGACAAAGA GGCACTTTAT 
AAAAAGGCAG TGTCCATCGC TGATAAGGAG GTGTTTGATT TCCGCATGGT GAACCATGTC 
GATGCAACGG AGGGTGACGC TTACACAGAC ATGCACATTG TGGACACTAG CGTAACCCTG 
ACCGTGGGCT GCATTCAAGT TATTTTCCTC AACAAGTTTA TGTCCTCAAT ACTGGCATTT 
ATTAATAATT TCCAGGAGGC TAAAAATGCA TTGGCGGAGG TGACGGCCCA GGCAGCAGAG 
AAAGCAGCCA CGGGGGTTCT GGAGCTGGCA GAGAGAAGTA CTCGGATCTC CCTGGACGTC 
GACATCAAAG CTCCTGTTGT CTTCCTCCCA CAATCCTCAG TTTCCCACAA CGTCATTGTG 
GCAGATCTGG GTTTAGTGAC TGTAAAGAAC CGCTTTGAGA TCGTCTCCTC TCCAACACAC 
ACCAAGATCC CTCCTGTCAT GGACATCATG GCTGTCGGAT TGAGTGATCT GAAAATGTAC 
AGGACAACGT TCACTGATGA CAAGTTTAAC AGCGAGATTC AGTTGCTCAA GCCAGTCAAC 
ATGGACCTGT CAATCAAACG CAATCTCTCC GCCTCCTGGT ATCACAGCAT ACCCGACATC 
CAGATCAATG CTCACCTCAA GCCCATGAGC CTGATCTTGA GTCAGGAGGA TCTGACTGTG 
GTGCTGAAGA CTCTGAGTGA GAATCTGTCC GAGACGTCTG ATGCTCCTCC AGCTCCGTCC 
AGCTCTGATA AAGGAGATAC GGTTTCTAAC AAAGGCTCCC AGGGTTCTGG AGGCAACACC 
GTTGTGACCG CAGCAGTGGT GGAGGGTCAG AAATCAGGCA AACTGAAGAA CACCCTAAAA 
CTGAACTTCA AGTTTGACTC CATGACGCTG GTCCTCTACA GCCCGAACAG CGAGATCCAG 
TTGTCCGACT CTCGTGATGA GAGTTTGAAG TTGGCCGAGT TCACACTGGG CACCATCTCC 
ACCAGTGTGC ACATGTTCTC CGACAGCTCC ATGAAGGCCT CGCTCAAACT CACCAACTGT 
CTGCTGGATG ACAAGAGGCA GGGAGTGAAG ATGATTACAT CCAGGATGAT GGCGATGCGT 
CAGGACCGAG AGGACAGTGT GATGGTGGAC GTCAGCTACC GCCAGAGTCG GGACGGGACT 
CGTCTGGACA CCCTCGTGCA AGACCTTTAT CTTTGTGCCA GTATGGAGTT TCTGCTCACT 
GTAGCTGATG TGTTTCTTAA AGCTCTGTCT GACGGCTTCT CCGGTCCTCC AAAGAGTAAC 
AAACAACCGG CCAGCACCTC TAAAGACCCA GCGGCTCCTG CTCCACCAGT GGTTGCTAAA 
ACCGAGATGA ATGTGGTGGT GAAGAACCCA GAGATCGTGT TTGTGGCGGA TCTGACGCGT 
GCTGAAGCTC CGGCTCTGGT CATGACCACT CAGTGTGAGC TGGTGATGAA GAGCGAGCCC 
AGCGGTCAGT GCATGACGGC CGCCATTTCA GACCTGAAGA TTGTGGCCTG TCCGTTCCTG 
CGGGAGAAGA GGGAGAACAA CGTGACGACG GTCCTACAGC CCTGCGAGGT TTTCTTCCAG 
AGCCGACAGT CAAACACTGA GCCGCAGTGC ATCGAGCTGT CCATCAGCGC ACTCACGCTC 
AAGGTGTCTC CGATCATCAT AAACACGGTC ATCACCATTC AGACGGCTCT AAGTCCTGCC 
GCTGAGACGC CGCAGGAGCT GGAGAGCCCC ATCCCACAGG ACCTATGGGA GCGCCGCAGC 
CTTCGCGACC TGAAGCTCTG GTTCCTGGAG GAGGAGGGCG AGGAGGTCAG CACGTCCGTC 
ACGCGTCTCA TTCCTCAGGG AGAGTCTCTG AAGATGAGCA TCGCCTCCAT CTGCCTGACG 
CTGGAGGCCG GAGTGGGACA CCGCACCGTC CCCATGCTGC TGGCCAAAGC CTCGTTCCAG 
GGGGACGTCA AGGACTGGAG CACTCTCATC AACCTGCACT GCCACCTCAG CATGGAGGTG 
AACTACTATA ATGAGATGAT GGGTTTATGG GAGCCGCTGT TGGAGCCGCT GGACGATGAG 
GATAAAGACG GTTTTAGACC CTGGACTCTC GCACTGAAGA TGAAGAAGAA GCCGGTGGTC 
AGTTCTGGGG TGTCTGATGA GGTGGACGGC GCTGTGCCTG ATTATAAAAC GGTCATCAGC 
TTCACTAGTA AAGACCAGCT TAACGTCACC CTGTCCAAGT GTGGTCTGCA CATGCTCAGT 
AATCTGGGAA CGGCTTTTGC AGAGGCCGCT AAACAGACGG CCGAGTGCTT TCAGACAGAT 
CAGACACCAT TCGTGGTGAA GAATCGCCTG GGTTTGGCGG TGTCTGTGCT GCACAGCGAA 
GTGTTTCGAC CCATCGGGAA GGAGTCTAAG GATCGTGTGG TGGAGCTCCC GGATGGAGAG 
AGTCTGAATT TGGACTATAC CCTCACCGCC GAGTCCAGCG ACCAGTTCTC TGCCATGACC 
AGCCTCAGTG CCAAAAACTA CATACAGCCC ACTCCAGTCG GCCACACGAC CGCAAGTGTG 
ATCCCGCTCA TCAAAACGGG CCGGGGCATG TACAGCGTCA TGCACAACGA GTCCCGCGTC 
ACTCGCTTCT TCGTCTGTCA GATTTACACC GTCGAGGGCA GCAAATATGT CAAGATTCGC 
TCTCCCTTCC AGATTATCAA CCACTTTTCA GTGCCGTTCC GTGTTTATGA AGGATCCGTG 
TTTCTGGGCG AGTCTCTGCC GACAGAGGAG TTCTGTGTGC CGCTGGGATC CTACAGAGCT 
GAGCTGAGTC TGCAGCCTGT GCCTGATGGC GGTGAAGAGT ACGAGCACTC CGAGAGCTTC 
ACCTACACTG AGGTTGTCCA GAAACCCGGA AAGTGTCTGG AGAAAAACTG CCATCTGCGT 
GGTGACAGCG CCCAGATTCT GAAGGTCAAC ATGGTTCCTC TTGCGGACAC AGTGACGTCC 
CACAGCAGCG AGGAGGAGTT CGATAAGCCC ATCGTACTGC ATTTGTGGCC CACCGTCCTG 
CTGCGCAACC TGCTGCCCTA CCCAATTACT TTCAAGCTAA AGGGCATCGG AGATTCCCCC 
TCGGTGTCGC TGAAGGAGGG CCACTCATCC CAGATCCACA CCGCCATCAT GGACTTCTCC 
GTGTTGGATC TGAGGCTGCT GGGATACTTG CAGCAGGACT GGGAGTCTCA GTACAGCCTC 
AGCAGTGAAC AAGACGAGAT CAGCTTCATT AAGTTTCGCT CGCTCGCACG TGAGGAGGAG 
ACTGAGAGCC GGCCGGAGCT GGACATCGCT ATGCATGTGA AGAAGGAGGA GGGTCAGGTT 
GTGCTGGCCG TACACTCGCC GTACTGGATG GTGAATAAAA CAAGCCGACT GCTGCAGTAC 
AAGGCGGATG ATATTCATCG CAAACACCCC AAGGATTACG ACATGCCGCT GCTCTTCTCC 
TTCAAGCCAC GCAACTTCCT GCAAAACAAC AAGATCCGCC TGATGATCTC AGAAAGTGAG 
CTGTCAGATG ATTTCTCCAT CGACACTGTC GGGAGTTACG GAGATGTCAA ATGCAAAGGC 
AGACAGAAGG ATTACTTGGT GGGAGTGAAG ATCGATGCTA GCAGCTTCAG CTTGACACGT 
TTGGTGACTT TTCTGCCATT CTACATGCTG GTGAACAGAA CCAAACATGT GATTCGGGTC 
TGTGAGGAGG GGCAGGAGAA CTGGACAGAA GCCCGACCCG GACAGGCTGC TGTTCCATTC 
TGGCCAGAAA GAGACTCAAA GAAGCTGAGA GTGAAGGTGG AGGGCAGTCA GTCGTCACCA 
CGCGCCTTTG ACTTCCACCA GCCAGAGAAC TGCCTCCTGC TGCATCTGGA CAAAAGCTTG 
GGTGGCATCA TTGTGGATGT GAATCTGACA GAGCATTCAG CTGTGATCCT CTTCTCTGAT 
TATCATGATG GAGCCGCTCC CTTCCTCCTC ATCAACCACA CTAAGGACGA GACGCTGCAG 
TACCACCAGA GCAACAGTAA GAGGAAGCCT TCGACGTGGT TTCAGGATGA AATACTGGAT 
TTATCAATGA GGGATGAGAC TGATTCCCTC TGCTCTCAGG CCGAGATCGA TGAACTGAGT 
CCAGGTCAGG CGGTTTACTA CACCTGGGCT GAACCAACCG GCTCCAGGAT CCTCAGCTGG 
AAACTGGGCA AATACAAAGG AGAGCTCAAG AGTGAAGAGG ATCAGTTGCT GGATGTGGAC 
AAGGAGGGCA AGTTGTTTTT GGTGTCGTTT TATGAAGGCC TCCAGCGTGT GGCGCTCTTC 
ACCAGCGAGC AGCGCATCTT CAAGAAGATC TGCGACAGTG AGAAGATAGA GCTGGCTCAG 
CAAGAAATCA GCATCTTGCT GCAGAACATG GGCATTTCGC TGGTCAACAA CAACTCCAGC 
CAGGAAGTGG CCTTCATTGG CATCACCAGC TCTGATGTTG TGTGGGAAAC CAAGCCGAAG 
AAGCGCAGCC GCTGGAAAGT TCTGAGTGTT AAAGAGGCAG CAGTTCTGGA GCAGCACTAC 
AGAGACTACA TGGAGAACGG CCCAGTGGAC AGCACCATCA TAGACCTGGA CAACGACTAC 
CAGGTGTTGT TTGCTGCTAA CGGTGTGGAC ATGAGGATCA TGCAGCCCTA CGATGCTCCA 
CTCAGAAGGA ACTTCCTGCC GGCGTTGAAG GTGGAGTACA GCGTTTCAGC CCGCCAGAAA 
ACATACAGAG TCCAGATCAA CAGGATCCAG ATCCAAAACC AGCTGCAAGG AGCCATATTC 
CCGTTTGTGT TTTACCCCAT CAAACCGCCA AAGTCTGTCA GCATGGACTC AGAGCCAAAA 
CCGCTGGCTG ACGTGAGCAT CATCACCAGA GCAGCAGGAC ACTCCGACAT TCTGCGCATC 
AAATATTTTA TGGTGTTGAT TCAGGAGATG GACCTGCGGC TGGATCTGGG CTTTCTGTAT 
GCCATACTTG AGCTCTTCAC TGCTGAACAC ACCAGTGCTG TGACGTCTGA ACAGGAGGTG 
GAGTTGTTTG ATAAAGACAT AGAGTATCTA AAGACTGAGT TAAATCATGT TTCAGCTACA 
GACACATCTC CCATCAGCCT CTACGAGTAC TTCCACATCT CTCCTATAAA GCTTCACTTG 
AGCTTGTCTC TGAGCAGTGG TGGTGAAGAC AGCAATAAGC AAGAGCGCGA GACGGAGCTG 
ATCCCAGTTC AGTCTCTCCA TCTGCTGCTC AAGAGCATCG GGGCTACACT TACAGACGCA 
CAGGACGTCC TCTTTAAACT GGGCTTCTTC GAGCTGAGGT ATCAGTTTCG CACCACTCAG 
CAGCTGCAGT GGGAGGTCGT TCGCCATTAC TCCAAACAGG CTATCAAGCA GATGTATGTC 
CTTGTTCTGG GTTTGGACGT TCTTGGGAAT CCATTCGGGC TGATCCGGGG CCTGTCTGAA 
GGCGTCGAGG CTTTCTTTTA CGAGCCCTAT CAGGGTGCAA TTCAGGGTCC AGAGGAGTTC 
ATGGAGGGCA TGGCTCTCGG CGTGAAGGCT CTGGTTGGAG GCGCTGTGGG TGGTCTGGCC 
GGAGCGGCGT CTCGCATCAC TGGAGCGATG GCTAAAGGTG TGGCGGCGAT GACGATGGAT 
GAAGAGTACC AGCAGAAGAG ACGAGAGGCC ATGAACAAAC AGCCCAGCGG ACTGAGAGAA 
GGACTGACAC GAGGAGGAAA AGGACTGGTG TCTGGCTTCG TGAGTGGCAT CACGGGCATT 
GTGACCAAAC CCATTAAAGG AGCTCAGAAG GAAGGAGCGG CAGGTTTCTT CAAGGGTATG 
GGTAAGGGTT TAGTTGGAGC TCTGACCAGA CCAACAGGGG GCATCATTGA CATGGCCAGC 
AGCACCTTCC AGGGCATCAA GAGAGCTGCA GAGCTGTCTC AGGATGTGGA GAGTCTGCGC 
CCTCCACGCT TCATCCACGA GGACGGAGTG ATCCGGCCGT ACAGAGAGAG AGAAGGCCTC 
GGCAGTCAGA TGCTGCAGAG TATTGAAAAC GGACGGTTTG CCAAGTACAG GTACTTTGCT 
CACGCTAAAG TCAACGAGTC TGATTTCCTG ATGATCACTA AAAGGGGGAT CTTTTTCGTG 
ACAAAGGGCA CGTTTGGTCA GTTGACATGT GAATGGCAGT ATCTGTTCGA TGAGTTTACT 
AAAGCACCCA TGATTGTGGA AGGCAGGAGA CTGCGCATCG AAGCGAAGGA GAGAGTCAAG 
TCAGTGTTTC ACGCCAAAGA GTTCGGGAAG ATCATCAATT TCAAAACTCC AGAATTAGCA 
AAGTGGGTTC TTGCCAAGCT TGAAGACGCT CGGGAGAATC TGCCCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS13A ( XM_005167334.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005167391.1 Danio rerio vacuolar protein sorting 13 homolog A (S. cerevisiae) (vps13a), transcript variant X1, mRNA.

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CloneID	ODa14675
Clone ID Related Accession (Same CDS sequence)	XM_005167334.4 , XM_005167334.3
Accession Version	XM_005167334.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	vacuolar protein sorting-associated protein 13A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005167334.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGTTTG AGTCGGTGGT GGTCGACGTG CTGAACCGGT TTCTGGGGGA CTATGTTGGA 
AACCTCGACA GCTCGCAGCT CAGCCTCGGC ATATGGGGAG GTGATGCCAT ATTGAGGAAC 
CTCGAGATTA AAGAAAATGC ATTGAGCCAG CTGGACATTC CTTTCAAAGT CAGAGCAGGA 
CACATTGGGC GTTTGGAGTT AAAGATCCCC TGGAAGAACC TGTACACTCA GTCTGTGGAG 
GCCACGCTGG ATGAAGTTTA CCTGCTCATC GTACCTACTG CTAGTATAAA GTATGACGCA 
GAGAAGGAGG AGCGGCAGCT GCAGGAGTCT CGGCAGAGGG AGCTCCAGCG CATCGAGGAG 
ACCAAACAGA AAGCAGCAGA AAAAGAGAAT CCAAAGGGTG AGAAGCAGGA CACATTTGTG 
GAGAAACTGG TAACACAGGT GATCAAAAAC CTGCAGGTCA AAATATCCAA CATTCACGTC 
CGCTATGAGG ATGATGTCAC CAATCCCAGT GTTCCTCTCT CCTTTGGGAT ATCATTACAA 
AACCTCAGTC TCCAGACGGC GGATCAGAAC TGGAACCCCT GTCTTCTGGA TGAGAAAGCC 
AAACTGTTTT TTAAGCTTGT ACGTCTGGAT AATCTGTTTG CGTACTGGAA CGTCAGTTCA 
GAGCTGTACT CCAACCACAA CCCTGAGGAG GCTCTGCGAT ACCTGAAGCA CAGCGTAGCC 
ATCCAGACTG CCATCCCTCA GGATTATCAC TTCATCTTTC GTCCAATATC TGCCAATGCC 
AAGTTGCGGA TGAACCCTCG CTCTGATGTA GACTTCTCCT CACCCAAAAT GGACCTGGTG 
GTCAACCTTC CTGAAGTGGC CGTGGAGCTC AGCCGACCAC AGTACGTCAG CATTCTGGAG 
CTGTTGGGGT CGGTGGATAT GATGACCCGT AACCTTCCTT ACAGGAAGTA TCGGCCATAT 
GTGGTGGTTC ACACTAACGC TCGTCAGTGG TGGCGATATG TGATCACGGG TGTTCTGGAG 
GTCAATGTGA AGCCTCGTCT TCACATGTGG TCTTGGCTGC ATATTCGTCG ACATCGGCAG 
CATGTGAAAA AGTACAGAGA GCTTTATAAG AATAAAATCA TCAGTAAGAA ACCGGCAGAG 
AGTCTGCTGA AGGAGCTGGA GGAGACTGAA AGAACATTAG ACATATTCAA CATCACAATG 
GCGAGACAGC AGGCTGAGAT GGAGGCCTCG AAGGCAGGGC TCAGGATTTA CCGGCCCGGG 
GTGAAGGTGG AGGAGGAACA GTCACAGGGC TGGTTTGGCT GGATGTGGAA CTGGTCTGGA 
GAAGCCAGTG CCACTCAAAC CAAAGACGTC AAAACTGGAG GTTTTGATGG GTTAATGACG 
CCTGCAGAGA AAGCCAAACT ATACGCTGCC ATCGGATACA GCGAAACTGC TGTCAACCCC 
AACCTGCCAA AAAATTTTGA AAACATGAAG ATGAATTTCC ATCTTCTCCG CCTGTCCCTG 
TCCATCAAAG AGAGCCGAGA GAAGGCGGAG ATCATCAAAC TGTCTGTCAT TGACCTGAAG 
GCCATGCTGA CTCAAAGACC CGCGGCTCAA GCTATAAAGA TCTCCGCTCA GCTGTCGTCG 
TTTGAAGTGT CCGGTCTCCC GCGGGAAAAG TCGGCCCCGA TCCTGCTTTC TCCACGTCAT 
GTGGACGGCA TGAAGGATAA AAGTCTGCTG AACCTCATGT TCGAGACCAA CCCGCTGGAC 
GCTCGTGCTG ACCAGAGACT GCGCATCGAG TCCCAGCCGC TGGAGATCAT CTATGATGCA 
ATGACCGTGA ACAGTATGTC CGCTTTCTTC ATGCCTCCTA AAGACCTGCA GCTGGATGAA 
CTGACCAACG CCACACTGAT GAAGCTGGAG CAGTTCAGAG ACAAAACCTC CACAGGCCTC 
ATGTACATCA TTGAGACGCA GAAAGTTCTG GATCTGAAAA TAAACCTCAT GGCCTCGTAT 
GTCATCGTAC CGGAAAACGG CTTTTATGAC CCCAAACACA ACCTTGCCAT TCTAGATCTG 
GGTCATTTTC AGATGTCAAG TCAGAGCCGA GCTGATCTTC CTCAGCTGTC TGCGGGCAGC 
AGTACTCTAG AGGACATCAT GTCTCGAGCG TACGACAGCT TCGACGTCCA GCTCAGCAGT 
CTGCAGTTCC TCTACTCCAA AGCAGGTGGA GACTGGAAGC GGGCTCGTCC GCTAAGACAG 
TCTGCTTTCC ACATCCTGGA GCCAGTGGAC CTGAAGGTGG TCTTTAGCAG AGCGATGGTG 
GTCACAGACT CCAGGATGCC CAAGTTTAAG ATGCACGGTG AGCTGCCTCT GCTGTCTCTG 
CGGATCTCTG ATGATAAAGT GCGTGGCGTT CTGGCTCTGC TCAGCAGTAT TCCTCTACCT 
GAGAGCCGGC CCACGCCTCA GTCCAGAAAC ACGCAGGCCA CTCCAAAGGC CTCCAAGCGA 
GGAAATACCC CTCAGCTCAC GCCCCGAAGG CCAGCTATAG ATGACCTGCG TGCGTCCATC 
ATATACGGCT CAGAGGAGGA GCTGTTCTTT GACGCCCCCA GTTCACCGAT CGAGGATCTG 
CCGTTCTTCG AGGCCTCGTC GGACTCTCGG CATGCTTCAG CAGCTGATAA ACGTCTGTCT 
ATGGTGCTCA AAGACCCCAA GAAGAACATG ACTGAGTTTC TGCTTAGTTT TGAGATCAAC 
AAGCTCTCGG TGCAGCTGTG CCGTGTGTCG AATAGACAGG AAGTGAGTGT TCTGCATCTG 
TTCATCGATG GATTGGGAAC AGAGCTCAAA CTTCGCACGT TTGACATGTC GTCGTACACT 
TTCCTGCGTG AGATCTGCCT CGAGTGCTCA GAATACTTGG ATTCTGAGGA CAAGAAAGTT 
CACCTTATCA CCACGCTGGA CAACACAGAG GAGGATCTGC TCACTCTGGA GTATGTCAAG 
GCAGACAAAA ATGCACCAGA TTTTAAAGCT ATGTACAAAA ATACAGAGCA ACTAATCAAG 
GTGAATTTCT CCTCTCTGGA CATTCACCTG CACACAGAGG CTCTCCTGAA CTCCATGAAC 
TTCCTAAATA ATCTTCTGCC ACCGATGAAG AAGGAGACCC AGGAGGAACA ACCAATAATT 
CCCAAGGAGG AAGAGGAGGA GGAGGAGGAA GAAGAAGAAG AGGAGGAGGC TAAAGTAGAG 
AATTCTGCTG TGGCCAAGAA GTCTTCCTAC AAGAAGTCAA AGTTTGAGGA TGTGGTGAAT 
TTGCACATCC GCGCAGACCT CAACTGTCTG AAGGTGTTCA TACGAGGAGA GAAAGCCAGG 
ATCTCTGAGA TCAGCATAGA GGGACTGGTT TCTGAAGTTC AGATGAAGAA GAAGTCTGTG 
GAGATTCTGG CTAACTTGAA GAACATCATC ATTCTGGATT GTGACAAAGA GGCACTTTAT 
AAAAAGGCAG TGTCCATCGC TGATAAGGAG GTGTTTGATT TCCGCATGGT GAACCATGTC 
GATGCAACGG AGGGTGACGC TTACACAGAC ATGCACATTG TGGACACTAG CGTAACCCTG 
ACCGTGGGCT GCATTCAAGT TATTTTCCTC AACAAGTTTA TGTCCTCAAT ACTGGCATTT 
ATTAATAATT TCCAGGAGGC TAAAAATGCA TTGGCGGAGG TGACGGCCCA GGCAGCAGAG 
AAAGCAGCCA CGGGGGTTCT GGAGCTGGCA GAGAGAAGTA CTCGGATCTC CCTGGACGTC 
GACATCAAAG CTCCTGTTGT CTTCCTCCCA CAATCCTCAG TTTCCCACAA CGTCATTGTG 
GCAGATCTGG GTTTAGTGAC TGTAAAGAAC CGCTTTGAGA TCGTCTCCTC TCCAACACAC 
ACCAAGATCC CTCCTGTCAT GGACATCATG GCTGTCGGAT TGAGTGATCT GAAAATGTAC 
AGGACAACGT TCACTGATGA CAAGTTTAAC AGCGAGATTC AGTTGCTCAA GCCAGTCAAC 
ATGGACCTGT CAATCAAACG CAATCTCTCC GCCTCCTGGT ATCACAGCAT ACCCGACATC 
CAGATCAATG CTCACCTCAA GCCCATGAGC CTGATCTTGA GTCAGGAGGA TCTGACTGTG 
GTGCTGAAGA CTCTGAGTGA GAATCTGTCC GAGACGTCTG ATGCTCCTCC AGCTCCGTCC 
AGCTCTGATA AAGGAGATAC GGTTTCTAAC AAAGGCTCCC AGGGTTCTGG AGGCAACACC 
GTTGTGACCG CAGCAGTGGT GGAGGGTCAG AAATCAGGCA AACTGAAGAA CACCCTAAAA 
CTGAACTTCA AGTTTGACTC CATGACGCTG GTCCTCTACA GCCCGAACAG CGAGATCCAG 
TTGTCCGACT CTCGTGATGA GAGTTTGAAG TTGGCCGAGT TCACACTGGG CACCATCTCC 
ACCAGTGTGC ACATGTTCTC CGACAGCTCC ATGAAGGCCT CGCTCAAACT CACCAACTGT 
CTGCTGGATG ACAAGAGGCA GGGAGTGAAG ATGATTACAT CCAGGATGAT GGCGATGCGT 
CAGGACCGAG AGGACAGTGT GATGGTGGAC GTCAGCTACC GCCAGAGTCG GGACGGGACT 
CGTCTGGACA CCCTCGTGCA AGACCTTTAT CTTTGTGCCA GTATGGAGTT TCTGCTCACT 
GTAGCTGATG TGTTTCTTAA AGCTCTGTCT GACGGCTTCT CCGGTCCTCC AAAGAGTAAC 
AAACAACCGG CCAGCACCTC TAAAGACCCA GCGGCTCCTG CTCCACCAGT GGTTGCTAAA 
ACCGAGATGA ATGTGGTGGT GAAGAACCCA GAGATCGTGT TTGTGGCGGA TCTGACGCGT 
GCTGAAGCTC CGGCTCTGGT CATGACCACT CAGTGTGAGC TGGTGATGAA GAGCGAGCCC 
AGCGGTCAGT GCATGACGGC CGCCATTTCA GACCTGAAGA TTGTGGCCTG TCCGTTCCTG 
CGGGAGAAGA GGGAGAACAA CGTGACGACG GTCCTACAGC CCTGCGAGGT TTTCTTCCAG 
AGCCGACAGT CAAACACTGA GCCGCAGTGC ATCGAGCTGT CCATCAGCGC ACTCACGCTC 
AAGGTGTCTC CGATCATCAT AAACACGGTC ATCACCATTC AGACGGCTCT AAGTCCTGCC 
GCTGAGACGC CGCAGGAGCT GGAGAGCCCC ATCCCACAGG ACCTATGGGA GCGCCGCAGC 
CTTCGCGACC TGAAGCTCTG GTTCCTGGAG GAGGAGGGCG AGGAGGTCAG CACGTCCGTC 
ACGCGTCTCA TTCCTCAGGG AGAGTCTCTG AAGATGAGCA TCGCCTCCAT CTGCCTGACG 
CTGGAGGCCG GAGTGGGACA CCGCACCGTC CCCATGCTGC TGGCCAAAGC CTCGTTCCAG 
GGGGACGTCA AGGACTGGAG CACTCTCATC AACCTGCACT GCCACCTCAG CATGGAGGTG 
AACTACTATA ATGAGATGAT GGGTTTATGG GAGCCGCTGT TGGAGCCGCT GGACGATGAG 
GATAAAGACG GTTTTAGACC CTGGACTCTC GCACTGAAGA TGAAGAAGAA GCCGGTGGTC 
AGTTCTGGGG TGTCTGATGA GGTGGACGGC GCTGTGCCTG ATTATAAAAC GGTCATCAGC 
TTCACTAGTA AAGACCAGCT TAACGTCACC CTGTCCAAGT GTGGTCTGCA CATGCTCAGT 
AATCTGGGAA CGGCTTTTGC AGAGGCCGCT AAACAGACGG CCGAGTGCTT TCAGACAGAT 
CAGACACCAT TCGTGGTGAA GAATCGCCTG GGTTTGGCGG TGTCTGTGCT GCACAGCGAA 
GTGTTTCGAC CCATCGGGAA GGAGTCTAAG GATCGTGTGG TGGAGCTCCC GGATGGAGAG 
AGTCTGAATT TGGACTATAC CCTCACCGCC GAGTCCAGCG ACCAGTTCTC TGCCATGACC 
AGCCTCAGTG CCAAAAACTA CATACAGCCC ACTCCAGTCG GCCACACGAC CGCAAGTGTG 
ATCCCGCTCA TCAAAACGGG CCGGGGCATG TACAGCGTCA TGCACAACGA GTCCCGCGTC 
ACTCGCTTCT TCGTCTGTCA GATTTACACC GTCGAGGGCA GCAAATATGT CAAGATTCGC 
TCTCCCTTCC AGATTATCAA CCACTTTTCA GTGCCGTTCC GTGTTTATGA AGGATCCGTG 
TTTCTGGGCG AGTCTCTGCC GACAGAGGAG TTCTGTGTGC CGCTGGGATC CTACAGAGCT 
GAGCTGAGTC TGCAGCCTGT GCCTGATGGC GGTGAAGAGT ACGAGCACTC CGAGAGCTTC 
ACCTACACTG AGGTTGTCCA GAAACCCGGA AAGTGTCTGG AGAAAAACTG CCATCTGCGT 
GGTGACAGCG CCCAGATTCT GAAGGTCAAC ATGGTTCCTC TTGCGGACAC AGTGACGTCC 
CACAGCAGCG AGGAGGAGTT CGATAAGCCC ATCGTACTGC ATTTGTGGCC CACCGTCCTG 
CTGCGCAACC TGCTGCCCTA CCCAATTACT TTCAAGCTAA AGGGCATCGG AGATTCCCCC 
TCGGTGTCGC TGAAGGAGGG CCACTCATCC CAGATCCACA CCGCCATCAT GGACTTCTCC 
GTGTTGGATC TGAGGCTGCT GGGATACTTG CAGCAGGACT GGGAGTCTCA GTACAGCCTC 
AGCAGTGAAC AAGACGAGAT CAGCTTCATT AAGTTTCGCT CGCTCGCACG TGAGGAGGAG 
ACTGAGAGCC GGCCGGAGCT GGACATCGCT ATGCATGTGA AGAAGGAGGA GGGTCAGGTT 
GTGCTGGCCG TACACTCGCC GTACTGGATG GTGAATAAAA CAAGCCGACT GCTGCAGTAC 
AAGGCGGATG ATATTCATCG CAAACACCCC AAGGATTACG ACATGCCGCT GCTCTTCTCC 
TTCAAGCCAC GCAACTTCCT GCAAAACAAC AAGATCCGCC TGATGATCTC AGAAAGTGAG 
CTGTCAGATG ATTTCTCCAT CGACACTGTC GGGAGTTACG GAGATGTCAA ATGCAAAGGC 
AGACAGAAGG ATTACTTGGT GGGAGTGAAG ATCGATGCTA GCAGCTTCAG CTTGACACGT 
TTGGTGACTT TTCTGCCATT CTACATGCTG GTGAACAGAA CCAAACATGT GATTCGGGTC 
TGTGAGGAGG GGCAGGAGAA CTGGACAGAA GCCCGACCCG GACAGGCTGC TGTTCCATTC 
TGGCCAGAAA GAGACTCAAA GAAGCTGAGA GTGAAGGTGG AGGGCAGTCA GTCGTCACCA 
CGCGCCTTTG ACTTCCACCA GCCAGAGAAC TGCCTCCTGC TGCATCTGGA CAAAAGCTTG 
GGTGGCATCA TTGTGGATGT GAATCTGACA GAGCATTCAG CTGTGATCCT CTTCTCTGAT 
TATCATGATG GAGCCGCTCC CTTCCTCCTC ATCAACCACA CTAAGGACGA GACGCTGCAG 
TACCACCAGA GCAACAGTAA GAGGAAGCCT TCGACGTGGT TTCAGGATGA AATACTGGAT 
TTATCAATGA GGGATGAGAC TGATTCCCTC TGCTCTCAGG CCGAGATCGA TGAACTGAGT 
CCAGGTCAGG CGGTTTACTA CACCTGGGCT GAACCAACCG GCTCCAGGAT CCTCAGCTGG 
AAACTGGGCA AATACAAAGG AGAGCTCAAG AGTGAAGAGG ATCAGTTGCT GGATGTGGAC 
AAGGAGGGCA AGTTGTTTTT GGTGTCGTTT TATGAAGGCC TCCAGCGTGT GGCGCTCTTC 
ACCAGCGAGC AGCGCATCTT CAAGAAGATC TGCGACAGTG AGAAGATAGA GCTGGCTCAG 
CAAGAAATCA GCATCTTGCT GCAGAACATG GGCATTTCGC TGGTCAACAA CAACTCCAGC 
CAGGAAGTGG CCTTCATTGG CATCACCAGC TCTGATGTTG TGTGGGAAAC CAAGCCGAAG 
AAGCGCAGCC GCTGGAAAGT TCTGAGTGTT AAAGAGGCAG CAGTTCTGGA GCAGCACTAC 
AGAGACTACA TGGAGAACGG CCCAGTGGAC AGCACCATCA TAGACCTGGA CAACGACTAC 
CAGGTGTTGT TTGCTGCTAA CGGTGTGGAC ATGAGGATCA TGCAGCCCTA CGATGCTCCA 
CTCAGAAGGA ACTTCCTGCC GGCGTTGAAG GTGGAGTACA GCGTTTCAGC CCGCCAGAAA 
ACATACAGAG TCCAGATCAA CAGGATCCAG ATCCAAAACC AGCTGCAAGG AGCCATATTC 
CCGTTTGTGT TTTACCCCAT CAAACCGCCA AAGTCTGTCA GCATGGACTC AGAGCCAAAA 
CCGCTGGCTG ACGTGAGCAT CATCACCAGA GCAGCAGGAC ACTCCGACAT TCTGCGCATC 
AAATATTTTA TGGTGTTGAT TCAGGAGATG GACCTGCGGC TGGATCTGGG CTTTCTGTAT 
GCCATACTTG AGCTCTTCAC TGCTGAACAC ACCAGTGCTG TGACGTCTGA ACAGGAGGTG 
GAGTTGTTTG ATAAAGACAT AGAGTATCTA AAGACTGAGT TAAATCATGT TTCAGCTACA 
GACACATCTC CCATCAGCCT CTACGAGTAC TTCCACATCT CTCCTATAAA GCTTCACTTG 
AGCTTGTCTC TGAGCAGTGG TGGTGAAGAC AGCAATAAGC AAGAGCGCGA GACGGAGCTG 
ATCCCAGTTC AGTCTCTCCA TCTGCTGCTC AAGAGCATCG GGGCTACACT TACAGACGCA 
CAGGACGTCC TCTTTAAACT GGGCTTCTTC GAGCTGAGGT ATCAGTTTCG CACCACTCAG 
CAGCTGCAGT GGGAGGTCGT TCGCCATTAC TCCAAACAGG CTATCAAGCA GATGTATGTC 
CTTGTTCTGG GTTTGGACGT TCTTGGGAAT CCATTCGGGC TGATCCGGGG CCTGTCTGAA 
GGCGTCGAGG CTTTCTTTTA CGAGCCCTAT CAGGGTGCAA TTCAGGGTCC AGAGGAGTTC 
ATGGAGGGCA TGGCTCTCGG CGTGAAGGCT CTGGTTGGAG GCGCTGTGGG TGGTCTGGCC 
GGAGCGGCGT CTCGCATCAC TGGAGCGATG GCTAAAGGTG TGGCGGCGAT GACGATGGAT 
GAAGAGTACC AGCAGAAGAG ACGAGAGGCC ATGAACAAAC AGCCCAGCGG ACTGAGAGAA 
GGACTGACAC GAGGAGGAAA AGGACTGGTG TCTGGCTTCG TGAGTGGCAT CACGGGCATT 
GTGACCAAAC CCATTAAAGG AGCTCAGAAG GAAGGAGCGG CAGGTTTCTT CAAGGGTATG 
GGTAAGGGTT TAGTTGGAGC TCTGACCAGA CCAACAGGGG GCATCATTGA CATGGCCAGC 
AGCACCTTCC AGGGCATCAA GAGAGCTGCA GAGCTGTCTC AGGATGTGGA GAGTCTGCGC 
CCTCCACGCT TCATCCACGA GGACGGAGTG ATCCGGCCGT ACAGAGAGAG AGAAGGCCTC 
GGCAGTCAGA TGCTGCAGAG TATTGAAAAC GGACGGTTTG CCAAGTACAG GTACTTTGCT 
CACGCTAAAG TCAACGAGTC TGATTTCCTG ATGATCACTA AAAGGGGGAT CTTTTTCGTG 
ACAAAGGGCA CGTTTGGTCA GTTGACATGT GAATGGCAGT ATCTGTTCGA TGAGTTTACT 
AAAGCACCCA TGATTGTGGA AGGCAGGAGA CTGCGCATCG AAGCGAAGGA GAGAGTCAAG 
TCAGTGTTTC ACGCCAAAGA GTTCGGGAAG ATCATCAATT TCAAAACTCC AGAATTAGCA 
AAGTGGGTTC TTGCCAAGCT TGAAGACGCT CGGGAGAATC TGCCCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167391.1
CDS	152..9862
Translation	MVFESVVVDVLNRFLGDYVGNLDSSQLSLGIWGGDAILRNLEIKENALSQLDIPFKVRAGHIGRLELKIPWKNLYTQSVEATLDEVYLLIVPTASIKYDAEKEERQLQESRQRELQRIEETKQKAAEKENPKGEKQDTFVEKLVTQVIKNLQVKISNIHVRYEDDVTNPSVPLSFGISLQNLSLQTADQNWNPCLLDEKAKLFFKLVRLDNLFAYWNVSSELYSNHNPEEALRYLKHSVAIQTAIPQDYHFIFRPISANAKLRMNPRSDVDFSSPKMDLVVNLPEVAVELSRPQYVSILELLGSVDMMTRNLPYRKYRPYVVVHTNARQWWRYVITGVLEVNVKPRLHMWSWLHIRRHRQHVKKYRELYKNKIISKKPAESLLKELEETERTLDIFNITMARQQAEMEASKAGLRIYRPGVKVEEEQSQGWFGWMWNWSGEASATQTKDVKTGGFDGLMTPAEKAKLYAAIGYSETAVNPNLPKNFENMKMNFHLLRLSLSIKESREKAEIIKLSVIDLKAMLTQRPAAQAIKISAQLSSFEVSGLPREKSAPILLSPRHVDGMKDKSLLNLMFETNPLDARADQRLRIESQPLEIIYDAMTVNSMSAFFMPPKDLQLDELTNATLMKLEQFRDKTSTGLMYIIETQKVLDLKINLMASYVIVPENGFYDPKHNLAILDLGHFQMSSQSRADLPQLSAGSSTLEDIMSRAYDSFDVQLSSLQFLYSKAGGDWKRARPLRQSAFHILEPVDLKVVFSRAMVVTDSRMPKFKMHGELPLLSLRISDDKVRGVLALLSSIPLPESRPTPQSRNTQATPKASKRGNTPQLTPRRPAIDDLRASIIYGSEEELFFDAPSSPIEDLPFFEASSDSRHASAADKRLSMVLKDPKKNMTEFLLSFEINKLSVQLCRVSNRQEVSVLHLFIDGLGTELKLRTFDMSSYTFLREICLECSEYLDSEDKKVHLITTLDNTEEDLLTLEYVKADKNAPDFKAMYKNTEQLIKVNFSSLDIHLHTEALLNSMNFLNNLLPPMKKETQEEQPIIPKEEEEEEEEEEEEEEAKVENSAVAKKSSYKKSKFEDVVNLHIRADLNCLKVFIRGEKARISEISIEGLVSEVQMKKKSVEILANLKNIIILDCDKEALYKKAVSIADKEVFDFRMVNHVDATEGDAYTDMHIVDTSVTLTVGCIQVIFLNKFMSSILAFINNFQEAKNALAEVTAQAAEKAATGVLELAERSTRISLDVDIKAPVVFLPQSSVSHNVIVADLGLVTVKNRFEIVSSPTHTKIPPVMDIMAVGLSDLKMYRTTFTDDKFNSEIQLLKPVNMDLSIKRNLSASWYHSIPDIQINAHLKPMSLILSQEDLTVVLKTLSENLSETSDAPPAPSSSDKGDTVSNKGSQGSGGNTVVTAAVVEGQKSGKLKNTLKLNFKFDSMTLVLYSPNSEIQLSDSRDESLKLAEFTLGTISTSVHMFSDSSMKASLKLTNCLLDDKRQGVKMITSRMMAMRQDREDSVMVDVSYRQSRDGTRLDTLVQDLYLCASMEFLLTVADVFLKALSDGFSGPPKSNKQPASTSKDPAAPAPPVVAKTEMNVVVKNPEIVFVADLTRAEAPALVMTTQCELVMKSEPSGQCMTAAISDLKIVACPFLREKRENNVTTVLQPCEVFFQSRQSNTEPQCIELSISALTLKVSPIIINTVITIQTALSPAAETPQELESPIPQDLWERRSLRDLKLWFLEEEGEEVSTSVTRLIPQGESLKMSIASICLTLEAGVGHRTVPMLLAKASFQGDVKDWSTLINLHCHLSMEVNYYNEMMGLWEPLLEPLDDEDKDGFRPWTLALKMKKKPVVSSGVSDEVDGAVPDYKTVISFTSKDQLNVTLSKCGLHMLSNLGTAFAEAAKQTAECFQTDQTPFVVKNRLGLAVSVLHSEVFRPIGKESKDRVVELPDGESLNLDYTLTAESSDQFSAMTSLSAKNYIQPTPVGHTTASVIPLIKTGRGMYSVMHNESRVTRFFVCQIYTVEGSKYVKIRSPFQIINHFSVPFRVYEGSVFLGESLPTEEFCVPLGSYRAELSLQPVPDGGEEYEHSESFTYTEVVQKPGKCLEKNCHLRGDSAQILKVNMVPLADTVTSHSSEEEFDKPIVLHLWPTVLLRNLLPYPITFKLKGIGDSPSVSLKEGHSSQIHTAIMDFSVLDLRLLGYLQQDWESQYSLSSEQDEISFIKFRSLAREEETESRPELDIAMHVKKEEGQVVLAVHSPYWMVNKTSRLLQYKADDIHRKHPKDYDMPLLFSFKPRNFLQNNKIRLMISESELSDDFSIDTVGSYGDVKCKGRQKDYLVGVKIDASSFSLTRLVTFLPFYMLVNRTKHVIRVCEEGQENWTEARPGQAAVPFWPERDSKKLRVKVEGSQSSPRAFDFHQPENCLLLHLDKSLGGIIVDVNLTEHSAVILFSDYHDGAAPFLLINHTKDETLQYHQSNSKRKPSTWFQDEILDLSMRDETDSLCSQAEIDELSPGQAVYYTWAEPTGSRILSWKLGKYKGELKSEEDQLLDVDKEGKLFLVSFYEGLQRVALFTSEQRIFKKICDSEKIELAQQEISILLQNMGISLVNNNSSQEVAFIGITSSDVVWETKPKKRSRWKVLSVKEAAVLEQHYRDYMENGPVDSTIIDLDNDYQVLFAANGVDMRIMQPYDAPLRRNFLPALKVEYSVSARQKTYRVQINRIQIQNQLQGAIFPFVFYPIKPPKSVSMDSEPKPLADVSIITRAAGHSDILRIKYFMVLIQEMDLRLDLGFLYAILELFTAEHTSAVTSEQEVELFDKDIEYLKTELNHVSATDTSPISLYEYFHISPIKLHLSLSLSSGGEDSNKQERETELIPVQSLHLLLKSIGATLTDAQDVLFKLGFFELRYQFRTTQQLQWEVVRHYSKQAIKQMYVLVLGLDVLGNPFGLIRGLSEGVEAFFYEPYQGAIQGPEEFMEGMALGVKALVGGAVGGLAGAASRITGAMAKGVAAMTMDEEYQQKRREAMNKQPSGLREGLTRGGKGLVSGFVSGITGIVTKPIKGAQKEGAAGFFKGMGKGLVGALTRPTGGIIDMASSTFQGIKRAAELSQDVESLRPPRFIHEDGVIRPYREREGLGSQMLQSIENGRFAKYRYFAHAKVNESDFLMITKRGIFFVTKGTFGQLTCEWQYLFDEFTKAPMIVEGRRLRIEAKERVKSVFHAKEFGKIINFKTPELAKWVLAKLEDARENLPK

Target ORF information:

RefSeq Version	XM_005167334.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio vacuolar protein sorting 13 homolog A (S. cerevisiae) (vps13a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167334.3

ORF Insert Sequence:

ATGGTGTTTG AGTCGGTGGT GGTCGACGTG CTGAACCGGT TTCTGGGGGA CTATGTTGGA 
AACCTCGACA GCTCGCAGCT CAGCCTCGGC ATATGGGGAG GTGATGCCAT ATTGAGGAAC 
CTCGAGATTA AAGAAAATGC ATTGAGCCAG CTGGACATTC CTTTCAAAGT CAGAGCAGGA 
CACATTGGGC GTTTGGAGTT AAAGATCCCC TGGAAGAACC TGTACACTCA GTCTGTGGAG 
GCCACGCTGG ATGAAGTTTA CCTGCTCATC GTACCTACTG CTAGTATAAA GTATGACGCA 
GAGAAGGAGG AGCGGCAGCT GCAGGAGTCT CGGCAGAGGG AGCTCCAGCG CATCGAGGAG 
ACCAAACAGA AAGCAGCAGA AAAAGAGAAT CCAAAGGGTG AGAAGCAGGA CACATTTGTG 
GAGAAACTGG TAACACAGGT GATCAAAAAC CTGCAGGTCA AAATATCCAA CATTCACGTC 
CGCTATGAGG ATGATGTCAC CAATCCCAGT GTTCCTCTCT CCTTTGGGAT ATCATTACAA 
AACCTCAGTC TCCAGACGGC GGATCAGAAC TGGAACCCCT GTCTTCTGGA TGAGAAAGCC 
AAACTGTTTT TTAAGCTTGT ACGTCTGGAT AATCTGTTTG CGTACTGGAA CGTCAGTTCA 
GAGCTGTACT CCAACCACAA CCCTGAGGAG GCTCTGCGAT ACCTGAAGCA CAGCGTAGCC 
ATCCAGACTG CCATCCCTCA GGATTATCAC TTCATCTTTC GTCCAATATC TGCCAATGCC 
AAGTTGCGGA TGAACCCTCG CTCTGATGTA GACTTCTCCT CACCCAAAAT GGACCTGGTG 
GTCAACCTTC CTGAAGTGGC CGTGGAGCTC AGCCGACCAC AGTACGTCAG CATTCTGGAG 
CTGTTGGGGT CGGTGGATAT GATGACCCGT AACCTTCCTT ACAGGAAGTA TCGGCCATAT 
GTGGTGGTTC ACACTAACGC TCGTCAGTGG TGGCGATATG TGATCACGGG TGTTCTGGAG 
GTCAATGTGA AGCCTCGTCT TCACATGTGG TCTTGGCTGC ATATTCGTCG ACATCGGCAG 
CATGTGAAAA AGTACAGAGA GCTTTATAAG AATAAAATCA TCAGTAAGAA ACCGGCAGAG 
AGTCTGCTGA AGGAGCTGGA GGAGACTGAA AGAACATTAG ACATATTCAA CATCACAATG 
GCGAGACAGC AGGCTGAGAT GGAGGCCTCG AAGGCAGGGC TCAGGATTTA CCGGCCCGGG 
GTGAAGGTGG AGGAGGAACA GTCACAGGGC TGGTTTGGCT GGATGTGGAA CTGGTCTGGA 
GAAGCCAGTG CCACTCAAAC CAAAGACGTC AAAACTGGAG GTTTTGATGG GTTAATGACG 
CCTGCAGAGA AAGCCAAACT ATACGCTGCC ATCGGATACA GCGAAACTGC TGTCAACCCC 
AACCTGCCAA AAAATTTTGA AAACATGAAG ATGAATTTCC ATCTTCTCCG CCTGTCCCTG 
TCCATCAAAG AGAGCCGAGA GAAGGCGGAG ATCATCAAAC TGTCTGTCAT TGACCTGAAG 
GCCATGCTGA CTCAAAGACC CGCGGCTCAA GCTATAAAGA TCTCCGCTCA GCTGTCGTCG 
TTTGAAGTGT CCGGTCTCCC GCGGGAAAAG TCGGCCCCGA TCCTGCTTTC TCCACGTCAT 
GTGGACGGCA TGAAGGATAA AAGTCTGCTG AACCTCATGT TCGAGACCAA CCCGCTGGAC 
GCTCGTGCTG ACCAGAGACT GCGCATCGAG TCCCAGCCGC TGGAGATCAT CTATGATGCA 
ATGACCGTGA ACAGTATGTC CGCTTTCTTC ATGCCTCCTA AAGACCTGCA GCTGGATGAA 
CTGACCAACG CCACACTGAT GAAGCTGGAG CAGTTCAGAG ACAAAACCTC CACAGGCCTC 
ATGTACATCA TTGAGACGCA GAAAGTTCTG GATCTGAAAA TAAACCTCAT GGCCTCGTAT 
GTCATCGTAC CGGAAAACGG CTTTTATGAC CCCAAACACA ACCTTGCCAT TCTAGATCTG 
GGTCATTTTC AGATGTCAAG TCAGAGCCGA GCTGATCTTC CTCAGCTGTC TGCGGGCAGC 
AGTACTCTAG AGGACATCAT GTCTCGAGCG TACGACAGCT TCGACGTCCA GCTCAGCAGT 
CTGCAGTTCC TCTACTCCAA AGCAGGTGGA GACTGGAAGC GGGCTCGTCC GCTAAGACAG 
TCTGCTTTCC ACATCCTGGA GCCAGTGGAC CTGAAGGTGG TCTTTAGCAG AGCGATGGTG 
GTCACAGACT CCAGGATGCC CAAGTTTAAG ATGCACGGTG AGCTGCCTCT GCTGTCTCTG 
CGGATCTCTG ATGATAAAGT GCGTGGCGTT CTGGCTCTGC TCAGCAGTAT TCCTCTACCT 
GAGAGCCGGC CCACGCCTCA GTCCAGAAAC ACGCAGGCCA CTCCAAAGGC CTCCAAGCGA 
GGAAATACCC CTCAGCTCAC GCCCCGAAGG CCAGCTATAG ATGACCTGCG TGCGTCCATC 
ATATACGGCT CAGAGGAGGA GCTGTTCTTT GACGCCCCCA GTTCACCGAT CGAGGATCTG 
CCGTTCTTCG AGGCCTCGTC GGACTCTCGG CATGCTTCAG CAGCTGATAA ACGTCTGTCT 
ATGGTGCTCA AAGACCCCAA GAAGAACATG ACTGAGTTTC TGCTTAGTTT TGAGATCAAC 
AAGCTCTCGG TGCAGCTGTG CCGTGTGTCG AATAGACAGG AAGTGAGTGT TCTGCATCTG 
TTCATCGATG GATTGGGAAC AGAGCTCAAA CTTCGCACGT TTGACATGTC GTCGTACACT 
TTCCTGCGTG AGATCTGCCT CGAGTGCTCA GAATACTTGG ATTCTGAGGA CAAGAAAGTT 
CACCTTATCA CCACGCTGGA CAACACAGAG GAGGATCTGC TCACTCTGGA GTATGTCAAG 
GCAGACAAAA ATGCACCAGA TTTTAAAGCT ATGTACAAAA ATACAGAGCA ACTAATCAAG 
GTGAATTTCT CCTCTCTGGA CATTCACCTG CACACAGAGG CTCTCCTGAA CTCCATGAAC 
TTCCTAAATA ATCTTCTGCC ACCGATGAAG AAGGAGACCC AGGAGGAACA ACCAATAATT 
CCCAAGGAGG AAGAGGAGGA GGAGGAGGAA GAAGAAGAAG AGGAGGAGGC TAAAGTAGAG 
AATTCTGCTG TGGCCAAGAA GTCTTCCTAC AAGAAGTCAA AGTTTGAGGA TGTGGTGAAT 
TTGCACATCC GCGCAGACCT CAACTGTCTG AAGGTGTTCA TACGAGGAGA GAAAGCCAGG 
ATCTCTGAGA TCAGCATAGA GGGACTGGTT TCTGAAGTTC AGATGAAGAA GAAGTCTGTG 
GAGATTCTGG CTAACTTGAA GAACATCATC ATTCTGGATT GTGACAAAGA GGCACTTTAT 
AAAAAGGCAG TGTCCATCGC TGATAAGGAG GTGTTTGATT TCCGCATGGT GAACCATGTC 
GATGCAACGG AGGGTGACGC TTACACAGAC ATGCACATTG TGGACACTAG CGTAACCCTG 
ACCGTGGGCT GCATTCAAGT TATTTTCCTC AACAAGTTTA TGTCCTCAAT ACTGGCATTT 
ATTAATAATT TCCAGGAGGC TAAAAATGCA TTGGCGGAGG TGACGGCCCA GGCAGCAGAG 
AAAGCAGCCA CGGGGGTTCT GGAGCTGGCA GAGAGAAGTA CTCGGATCTC CCTGGACGTC 
GACATCAAAG CTCCTGTTGT CTTCCTCCCA CAATCCTCAG TTTCCCACAA CGTCATTGTG 
GCAGATCTGG GTTTAGTGAC TGTAAAGAAC CGCTTTGAGA TCGTCTCCTC TCCAACACAC 
ACCAAGATCC CTCCTGTCAT GGACATCATG GCTGTCGGAT TGAGTGATCT GAAAATGTAC 
AGGACAACGT TCACTGATGA CAAGTTTAAC AGCGAGATTC AGTTGCTCAA GCCAGTCAAC 
ATGGACCTGT CAATCAAACG CAATCTCTCC GCCTCCTGGT ATCACAGCAT ACCCGACATC 
CAGATCAATG CTCACCTCAA GCCCATGAGC CTGATCTTGA GTCAGGAGGA TCTGACTGTG 
GTGCTGAAGA CTCTGAGTGA GAATCTGTCC GAGACGTCTG ATGCTCCTCC AGCTCCGTCC 
AGCTCTGATA AAGGAGATAC GGTTTCTAAC AAAGGCTCCC AGGGTTCTGG AGGCAACACC 
GTTGTGACCG CAGCAGTGGT GGAGGGTCAG AAATCAGGCA AACTGAAGAA CACCCTAAAA 
CTGAACTTCA AGTTTGACTC CATGACGCTG GTCCTCTACA GCCCGAACAG CGAGATCCAG 
TTGTCCGACT CTCGTGATGA GAGTTTGAAG TTGGCCGAGT TCACACTGGG CACCATCTCC 
ACCAGTGTGC ACATGTTCTC CGACAGCTCC ATGAAGGCCT CGCTCAAACT CACCAACTGT 
CTGCTGGATG ACAAGAGGCA GGGAGTGAAG ATGATTACAT CCAGGATGAT GGCGATGCGT 
CAGGACCGAG AGGACAGTGT GATGGTGGAC GTCAGCTACC GCCAGAGTCG GGACGGGACT 
CGTCTGGACA CCCTCGTGCA AGACCTTTAT CTTTGTGCCA GTATGGAGTT TCTGCTCACT 
GTAGCTGATG TGTTTCTTAA AGCTCTGTCT GACGGCTTCT CCGGTCCTCC AAAGAGTAAC 
AAACAACCGG CCAGCACCTC TAAAGACCCA GCGGCTCCTG CTCCACCAGT GGTTGCTAAA 
ACCGAGATGA ATGTGGTGGT GAAGAACCCA GAGATCGTGT TTGTGGCGGA TCTGACGCGT 
GCTGAAGCTC CGGCTCTGGT CATGACCACT CAGTGTGAGC TGGTGATGAA GAGCGAGCCC 
AGCGGTCAGT GCATGACGGC CGCCATTTCA GACCTGAAGA TTGTGGCCTG TCCGTTCCTG 
CGGGAGAAGA GGGAGAACAA CGTGACGACG GTCCTACAGC CCTGCGAGGT TTTCTTCCAG 
AGCCGACAGT CAAACACTGA GCCGCAGTGC ATCGAGCTGT CCATCAGCGC ACTCACGCTC 
AAGGTGTCTC CGATCATCAT AAACACGGTC ATCACCATTC AGACGGCTCT AAGTCCTGCC 
GCTGAGACGC CGCAGGAGCT GGAGAGCCCC ATCCCACAGG ACCTATGGGA GCGCCGCAGC 
CTTCGCGACC TGAAGCTCTG GTTCCTGGAG GAGGAGGGCG AGGAGGTCAG CACGTCCGTC 
ACGCGTCTCA TTCCTCAGGG AGAGTCTCTG AAGATGAGCA TCGCCTCCAT CTGCCTGACG 
CTGGAGGCCG GAGTGGGACA CCGCACCGTC CCCATGCTGC TGGCCAAAGC CTCGTTCCAG 
GGGGACGTCA AGGACTGGAG CACTCTCATC AACCTGCACT GCCACCTCAG CATGGAGGTG 
AACTACTATA ATGAGATGAT GGGTTTATGG GAGCCGCTGT TGGAGCCGCT GGACGATGAG 
GATAAAGACG GTTTTAGACC CTGGACTCTC GCACTGAAGA TGAAGAAGAA GCCGGTGGTC 
AGTTCTGGGG TGTCTGATGA GGTGGACGGC GCTGTGCCTG ATTATAAAAC GGTCATCAGC 
TTCACTAGTA AAGACCAGCT TAACGTCACC CTGTCCAAGT GTGGTCTGCA CATGCTCAGT 
AATCTGGGAA CGGCTTTTGC AGAGGCCGCT AAACAGACGG CCGAGTGCTT TCAGACAGAT 
CAGACACCAT TCGTGGTGAA GAATCGCCTG GGTTTGGCGG TGTCTGTGCT GCACAGCGAA 
GTGTTTCGAC CCATCGGGAA GGAGTCTAAG GATCGTGTGG TGGAGCTCCC GGATGGAGAG 
AGTCTGAATT TGGACTATAC CCTCACCGCC GAGTCCAGCG ACCAGTTCTC TGCCATGACC 
AGCCTCAGTG CCAAAAACTA CATACAGCCC ACTCCAGTCG GCCACACGAC CGCAAGTGTG 
ATCCCGCTCA TCAAAACGGG CCGGGGCATG TACAGCGTCA TGCACAACGA GTCCCGCGTC 
ACTCGCTTCT TCGTCTGTCA GATTTACACC GTCGAGGGCA GCAAATATGT CAAGATTCGC 
TCTCCCTTCC AGATTATCAA CCACTTTTCA GTGCCGTTCC GTGTTTATGA AGGATCCGTG 
TTTCTGGGCG AGTCTCTGCC GACAGAGGAG TTCTGTGTGC CGCTGGGATC CTACAGAGCT 
GAGCTGAGTC TGCAGCCTGT GCCTGATGGC GGTGAAGAGT ACGAGCACTC CGAGAGCTTC 
ACCTACACTG AGGTTGTCCA GAAACCCGGA AAGTGTCTGG AGAAAAACTG CCATCTGCGT 
GGTGACAGCG CCCAGATTCT GAAGGTCAAC ATGGTTCCTC TTGCGGACAC AGTGACGTCC 
CACAGCAGCG AGGAGGAGTT CGATAAGCCC ATCGTACTGC ATTTGTGGCC CACCGTCCTG 
CTGCGCAACC TGCTGCCCTA CCCAATTACT TTCAAGCTAA AGGGCATCGG AGATTCCCCC 
TCGGTGTCGC TGAAGGAGGG CCACTCATCC CAGATCCACA CCGCCATCAT GGACTTCTCC 
GTGTTGGATC TGAGGCTGCT GGGATACTTG CAGCAGGACT GGGAGTCTCA GTACAGCCTC 
AGCAGTGAAC AAGACGAGAT CAGCTTCATT AAGTTTCGCT CGCTCGCACG TGAGGAGGAG 
ACTGAGAGCC GGCCGGAGCT GGACATCGCT ATGCATGTGA AGAAGGAGGA GGGTCAGGTT 
GTGCTGGCCG TACACTCGCC GTACTGGATG GTGAATAAAA CAAGCCGACT GCTGCAGTAC 
AAGGCGGATG ATATTCATCG CAAACACCCC AAGGATTACG ACATGCCGCT GCTCTTCTCC 
TTCAAGCCAC GCAACTTCCT GCAAAACAAC AAGATCCGCC TGATGATCTC AGAAAGTGAG 
CTGTCAGATG ATTTCTCCAT CGACACTGTC GGGAGTTACG GAGATGTCAA ATGCAAAGGC 
AGACAGAAGG ATTACTTGGT GGGAGTGAAG ATCGATGCTA GCAGCTTCAG CTTGACACGT 
TTGGTGACTT TTCTGCCATT CTACATGCTG GTGAACAGAA CCAAACATGT GATTCGGGTC 
TGTGAGGAGG GGCAGGAGAA CTGGACAGAA GCCCGACCCG GACAGGCTGC TGTTCCATTC 
TGGCCAGAAA GAGACTCAAA GAAGCTGAGA GTGAAGGTGG AGGGCAGTCA GTCGTCACCA 
CGCGCCTTTG ACTTCCACCA GCCAGAGAAC TGCCTCCTGC TGCATCTGGA CAAAAGCTTG 
GGTGGCATCA TTGTGGATGT GAATCTGACA GAGCATTCAG CTGTGATCCT CTTCTCTGAT 
TATCATGATG GAGCCGCTCC CTTCCTCCTC ATCAACCACA CTAAGGACGA GACGCTGCAG 
TACCACCAGA GCAACAGTAA GAGGAAGCCT TCGACGTGGT TTCAGGATGA AATACTGGAT 
TTATCAATGA GGGATGAGAC TGATTCCCTC TGCTCTCAGG CCGAGATCGA TGAACTGAGT 
CCAGGTCAGG CGGTTTACTA CACCTGGGCT GAACCAACCG GCTCCAGGAT CCTCAGCTGG 
AAACTGGGCA AATACAAAGG AGAGCTCAAG AGTGAAGAGG ATCAGTTGCT GGATGTGGAC 
AAGGAGGGCA AGTTGTTTTT GGTGTCGTTT TATGAAGGCC TCCAGCGTGT GGCGCTCTTC 
ACCAGCGAGC AGCGCATCTT CAAGAAGATC TGCGACAGTG AGAAGATAGA GCTGGCTCAG 
CAAGAAATCA GCATCTTGCT GCAGAACATG GGCATTTCGC TGGTCAACAA CAACTCCAGC 
CAGGAAGTGG CCTTCATTGG CATCACCAGC TCTGATGTTG TGTGGGAAAC CAAGCCGAAG 
AAGCGCAGCC GCTGGAAAGT TCTGAGTGTT AAAGAGGCAG CAGTTCTGGA GCAGCACTAC 
AGAGACTACA TGGAGAACGG CCCAGTGGAC AGCACCATCA TAGACCTGGA CAACGACTAC 
CAGGTGTTGT TTGCTGCTAA CGGTGTGGAC ATGAGGATCA TGCAGCCCTA CGATGCTCCA 
CTCAGAAGGA ACTTCCTGCC GGCGTTGAAG GTGGAGTACA GCGTTTCAGC CCGCCAGAAA 
ACATACAGAG TCCAGATCAA CAGGATCCAG ATCCAAAACC AGCTGCAAGG AGCCATATTC 
CCGTTTGTGT TTTACCCCAT CAAACCGCCA AAGTCTGTCA GCATGGACTC AGAGCCAAAA 
CCGCTGGCTG ACGTGAGCAT CATCACCAGA GCAGCAGGAC ACTCCGACAT TCTGCGCATC 
AAATATTTTA TGGTGTTGAT TCAGGAGATG GACCTGCGGC TGGATCTGGG CTTTCTGTAT 
GCCATACTTG AGCTCTTCAC TGCTGAACAC ACCAGTGCTG TGACGTCTGA ACAGGAGGTG 
GAGTTGTTTG ATAAAGACAT AGAGTATCTA AAGACTGAGT TAAATCATGT TTCAGCTACA 
GACACATCTC CCATCAGCCT CTACGAGTAC TTCCACATCT CTCCTATAAA GCTTCACTTG 
AGCTTGTCTC TGAGCAGTGG TGGTGAAGAC AGCAATAAGC AAGAGCGCGA GACGGAGCTG 
ATCCCAGTTC AGTCTCTCCA TCTGCTGCTC AAGAGCATCG GGGCTACACT TACAGACGCA 
CAGGACGTCC TCTTTAAACT GGGCTTCTTC GAGCTGAGGT ATCAGTTTCG CACCACTCAG 
CAGCTGCAGT GGGAGGTCGT TCGCCATTAC TCCAAACAGG CTATCAAGCA GATGTATGTC 
CTTGTTCTGG GTTTGGACGT TCTTGGGAAT CCATTCGGGC TGATCCGGGG CCTGTCTGAA 
GGCGTCGAGG CTTTCTTTTA CGAGCCCTAT CAGGGTGCAA TTCAGGGTCC AGAGGAGTTC 
ATGGAGGGCA TGGCTCTCGG CGTGAAGGCT CTGGTTGGAG GCGCTGTGGG TGGTCTGGCC 
GGAGCGGCGT CTCGCATCAC TGGAGCGATG GCTAAAGGTG TGGCGGCGAT GACGATGGAT 
GAAGAGTACC AGCAGAAGAG ACGAGAGGCC ATGAACAAAC AGCCCAGCGG ACTGAGAGAA 
GGACTGACAC GAGGAGGAAA AGGACTGGTG TCTGGCTTCG TGAGTGGCAT CACGGGCATT 
GTGACCAAAC CCATTAAAGG AGCTCAGAAG GAAGGAGCGG CAGGTTTCTT CAAGGGTATG 
GGTAAGGGTT TAGTTGGAGC TCTGACCAGA CCAACAGGGG GCATCATTGA CATGGCCAGC 
AGCACCTTCC AGGGCATCAA GAGAGCTGCA GAGCTGTCTC AGGATGTGGA GAGTCTGCGC 
CCTCCACGCT TCATCCACGA GGACGGAGTG ATCCGGCCGT ACAGAGAGAG AGAAGGCCTC 
GGCAGTCAGA TGCTGCAGAG TATTGAAAAC GGACGGTTTG CCAAGTACAG GTACTTTGCT 
CACGCTAAAG TCAACGAGTC TGATTTCCTG ATGATCACTA AAAGGGGGAT CTTTTTCGTG 
ACAAAGGGCA CGTTTGGTCA GTTGACATGT GAATGGCAGT ATCTGTTCGA TGAGTTTACT 
AAAGCACCCA TGATTGTGGA AGGCAGGAGA CTGCGCATCG AAGCGAAGGA GAGAGTCAAG 
TCAGTGTTTC ACGCCAAAGA GTTCGGGAAG ATCATCAATT TCAAAACTCC AGAATTAGCA 
AAGTGGGTTC TTGCCAAGCT TGAAGACGCT CGGGAGAATC TGCCCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS13A ( XM_009304309.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009302584.1 Danio rerio vacuolar protein sorting 13 homolog A (S. cerevisiae) (vps13a), transcript variant X2, mRNA.

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CloneID	ODa40321
Clone ID Related Accession (Same CDS sequence)	XM_009304309.3 , XM_009304309.2
Accession Version	XM_009304309.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	vacuolar protein sorting-associated protein 13A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304309.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGTTTG AGTCGGTGGT GGTCGACGTG CTGAACCGGT TTCTGGGGGA CTATGTTGGA 
AACCTCGACA GCTCGCAGCT CAGCCTCGGC ATATGGGGAG GTGATGCCAT ATTGAGGAAC 
CTCGAGATTA AAGAAAATGC ATTGAGCCAG CTGGACATTC CTTTCAAAGT CAGAGCAGGA 
CACATTGGGC GTTTGGAGTT AAAGATCCCC TGGAAGAACC TGTACACTCA GTCTGTGGAG 
GCCACGCTGG ATGAAGTTTA CCTGCTCATC GTACCTACTG CTAGTATAAA GTATGACGCA 
GAGAAGGAGG AGCGGCAGCT GCAGGAGTCT CGGCAGAGGG AGCTCCAGCG CATCGAGGAG 
ACCAAACAGA AAGCAGCAGA AAAAGAGAAT CCAAAGGGTG AGAAGCAGGA CACATTTGTG 
GAGAAACTGG TAACACAGGT GATCAAAAAC CTGCAGGTCA AAATATCCAA CATTCACGTC 
CGCTATGAGG ATGATGTCAC CAATCCCAGT GTTCCTCTCT CCTTTGGGAT ATCATTACAA 
AACCTCAGTC TCCAGACGGC GGATCAGAAC TGGAACCCCT GTCTTCTGGA TGAGAAAGCC 
AAACTGTTTT TTAAGCTTGT ACGTCTGGAT AATCTGTTTG CGTACTGGAA CGTCAGTTCA 
GAGCTGTACT CCAACCACAA CCCTGAGGAG GCTCTGCGAT ACCTGAAGCA CAGCGTAGCC 
ATCCAGACTG CCATCCCTCA GGATTATCAC TTCATCTTTC GTCCAATATC TGCCAATGCC 
AAGTTGCGGA TGAACCCTCG CTCTGATGTA GACTTCTCCT CACCCAAAAT GGACCTGGTG 
GTCAACCTTC CTGAAGTGGC CGTGGAGCTC AGCCGACCAC AGTACGTCAG CATTCTGGAG 
CTGTTGGGGT CGGTGGATAT GATGACCCGT AACCTTCCTT ACAGGAAGTA TCGGCCATAT 
GTGGTGGTTC ACACTAACGC TCGTCAGTGG TGGCGATATG TGATCACGGG TGTTCTGGAG 
GTCAATGTGA AGCCTCGTCT TCACATGTGG TCTTGGCTGC ATATTCGTCG ACATCGGCAG 
CATGTGAAAA AGTACAGAGA GCTTTATAAG AATAAAATCA TCAGTAAGAA ACCGGCAGAG 
AGTCTGCTGA AGGAGCTGGA GGAGACTGAA AGAACATTAG ACATATTCAA CATCACAATG 
GCGAGACAGC AGGCTGAGAT GGAGGCCTCG AAGGCAGGGC TCAGGATTTA CCGGCCCGGG 
GTGAAGGTGG AGGAGGAACA GTCACAGGGC TGGTTTGGCT GGATGTGGAA CTGGTCTGGA 
GAAGCCAGTG CCACTCAAAC CAAAGACGTC AAAACTGGAG GTTTTGATGG GTTAATGACG 
CCTGCAGAGA AAGCCAAACT ATACGCTGCC ATCGGATACA GCGAAACTGC TGTCAACCCC 
AACCTGCCAA AAAATTTTGA AAACATGAAG ATGAATTTCC ATCTTCTCCG CCTGTCCCTG 
TCCATCAAAG AGAGCCGAGA GAAGGCGGAG ATCATCAAAC TGTCTGTCAT TGACCTGAAG 
GCCATGCTGA CTCAAAGACC CGCGGCTCAA GCTATAAAGA TCTCCGCTCA GCTGTCGTCG 
TTTGAAGTGT CCGGTCTCCC GCGGGAAAAG TCGGCCCCGA TCCTGCTTTC TCCACGTCAT 
GTGGACGGCA TGAAGGATAA AAGTCTGCTG AACCTCATGT TCGAGACCAA CCCGCTGGAC 
GCTCGTGCTG ACCAGAGACT GCGCATCGAG TCCCAGCCGC TGGAGATCAT CTATGATGCA 
ATGACCGTGA ACAGTATGTC CGCTTTCTTC ATGCCTCCTA AAGACCTGCA GCTGGATGAA 
CTGACCAACG CCACACTGAT GAAGCTGGAG CAGTTCAGAG ACAAAACCTC CACAGGCCTC 
ATGTACATCA TTGAGACGCA GAAAGTTCTG GATCTGAAAA TAAACCTCAT GGCCTCGTAT 
GTCATCGTAC CGGAAAACGG CTTTTATGAC CCCAAACACA ACCTTGCCAT TCTAGATCTG 
GGTCATTTTC AGATGTCAAG TCAGAGCCGA GCTGATCTTC CTCAGCTGTC TGCGGGCAGC 
AGTACTCTAG AGGACATCAT GTCTCGAGCG TACGACAGCT TCGACGTCCA GCTCAGCAGT 
CTGCAGTTCC TCTACTCCAA AGCAGGTGGA GACTGGAAGC GGGCTCGTCC GCTAAGACAG 
TCTGCTTTCC ACATCCTGGA GCCAGTGGAC CTGAAGGTGG TCTTTAGCAG AGCGATGGTG 
GTCACAGACT CCAGGATGCC CAAGTTTAAG ATGCACGGTG AGCTGCCTCT GCTGTCTCTG 
CGGATCTCTG ATGATAAAGT GCGTGGCGTT CTGGCTCTGC TCAGCAGTAT TCCTCTACCT 
GAGAGCCGGC CCACGCCTCA GTCCAGAAAC ACGCAGGCCA CTCCAAAGGC CTCCAAGCGA 
GGAAATACCC CTCAGCTCAC GCCCCGAAGG CCAGCTATAG ATGACCTGCG TGCGTCCATC 
ATATACGGCT CAGAGGAGGA GCTGTTCTTT GACGCCCCCA GTTCACCGAT CGAGGATCTG 
CCGTTCTTCG AGGCCTCGTC GGACTCTCGG CATGCTTCAG CAGCTGATAA ACGTCTGTCT 
ATGGTGCTCA AAGACCCCAA GAAGAACATG ACTGAGTTTC TGCTTAGTTT TGAGATCAAC 
AAGCTCTCGG TGCAGCTGTG CCGTGTGTCG AATAGACAGG AAGTGAGTGT TCTGCATCTG 
TTCATCGATG GATTGGGAAC AGAGCTCAAA CTTCGCACGT TTGACATGTC GTCGTACACT 
TTCCTGCGTG AGATCTGCCT CGAGTGCTCA GAATACTTGG ATTCTGAGGA CAAGAAAGTT 
CACCTTATCA CCACGCTGGA CAACACAGAG GAGGATCTGC TCACTCTGGA GTATGTCAAG 
GCAGACAAAA ATGCACCAGA TTTTAAAGCT ATGTACAAAA ATACAGAGCA ACTAATCAAG 
GTGAATTTCT CCTCTCTGGA CATTCACCTG CACACAGAGG CTCTCCTGAA CTCCATGAAC 
TTCCTAAATA ATCTTCTGCC ACCGATGAAG AAGGAGACCC AGGAGGAACA ACCAATAATT 
CCCAAGGAGG AAGAGGAGGA GGAGGAGGAA GAAGAAGAAG AGGAGGAGGC TAAAGTAGAG 
AATTCTGCTG TGGCCAAGAA GTCTTCCTAC AAGAAGTCAA AGTTTGAGGA TGTGGTGAAT 
TTGCACATCC GCGCAGACCT CAACTGTCTG AAGGTGTTCA TACGAGGAGA GAAAGCCAGG 
ATCTCTGAGA TCAGCATAGA GGGACTGGTT TCTGAAGTTC AGATGAAGAA GAAGTCTGTG 
GAGATTCTGG CTAACTTGAA GAACATCATC ATTCTGGATT GTGACAAAGA GGCACTTTAT 
AAAAAGGCAG TGTCCATCGC TGATAAGGAG GTGTTTGATT TCCGCATGGT GAACCATGTC 
GATGCAACGG AGGGTGACGC TTACACAGAC ATGCACATTG TGGACACTAG CGTAACCCTG 
ACCGTGGGCT GCATTCAAGT TATTTTCCTC AACAAGTTTA TGTCCTCAAT ACTGGCATTT 
ATTAATAATT TCCAGGAGGC TAAAAATGCA TTGGCGGAGG TGACGGCCCA GGCAGCAGAG 
AAAGCAGCCA CGGGGGTTCT GGAGCTGGCA GAGAGAAGTA CTCGGATCTC CCTGGACGTC 
GACATCAAAG CTCCTGTTGT CTTCCTCCCA CAATCCTCAG TTTCCCACAA CGTCATTGTG 
GCAGATCTGG GTTTAGTGAC TGTAAAGAAC CGCTTTGAGA TCGTCTCCTC TCCAACACAC 
ACCAAGATCC CTCCTGTCAT GGACATCATG GCTGTCGGAT TGAGTGATCT GAAAATGTAC 
AGGACAACGT TCACTGATGA CAAGTTTAAC AGCGAGATTC AGTTGCTCAA GCCAGTCAAC 
ATGGACCTGT CAATCAAACG CAATCTCTCC GCCTCCTGGT ATCACAGCAT ACCCGACATC 
CAGATCAATG CTCACCTCAA GCCCATGAGC CTGATCTTGA GTCAGGAGGA TCTGACTGTG 
GTGCTGAAGA CTCTGAGTGA GAATCTGTCC GAGACGTCTG ATGCTCCTCC AGCTCCGTCC 
AGCTCTGATA AAGGAGATAC GGTTTCTAAC AAAGGCTCCC AGGGTTCTGG AGGCAACACC 
GTTGTGACCG CAGCAGTGGT GGAGGGTCAG AAATCAGGCA AACTGAAGAA CACCCTAAAA 
CTGAACTTCA AGTTTGACTC CATGACGCTG GTCCTCTACA GCCCGAACAG CGAGATCCAG 
TTGTCCGACT CTCGTGATGA GAGTTTGAAG TTGGCCGAGT TCACACTGGG CACCATCTCC 
ACCAGTGTGC ACATGTTCTC CGACAGCTCC ATGAAGGCCT CGCTCAAACT CACCAACTGT 
CTGCTGGATG ACAAGAGGCA GGGAGTGAAG ATGATTACAT CCAGGATGAT GGCGATGCGT 
CAGGACCGAG AGGACAGTGT GATGGTGGAC GTCAGCTACC GCCAGAGTCG GGACGGGACT 
CGTCTGGACA CCCTCGTGCA AGACCTTTAT CTTTGTGCCA GTATGGAGTT TCTGCTCACT 
GTAGCTGATG TGTTTCTTAA AGCTCTGTCT GACGGCTTCT CCGGTCCTCC AAAGAGTAAC 
AAACAACCGG CCAGCACCTC TAAAGACCCA GCGGCTCCTG CTCCACCAGT GGTTGCTAAA 
ACCGAGATGA ATGTGGTGGT GAAGAACCCA GAGATCGTGT TTGTGGCGGA TCTGACGCGT 
GCTGAAGCTC CGGCTCTGGT CATGACCACT CAGTGTGAGC TGGTGATGAA GAGCGAGCCC 
AGCGGTCAGT GCATGACGGC CGCCATTTCA GACCTGAAGA TTGTGGCCTG TCCGTTCCTG 
CGGGAGAAGA GGGAGAACAA CGTGACGACG GTCCTACAGC CCTGCGAGGT TTTCTTCCAG 
AGCCGACAGT CAAACACTGA GCCGCAGTGC ATCGAGCTGT CCATCAGCGC ACTCACGCTC 
AAGGTGTCTC CGATCATCAT AAACACGGTC ATCACCATTC AGACGGCTCT AAGTCCTGCC 
GCTGAGACGC CGCAGGAGCT GGAGAGCCCC ATCCCACAGG ACCTATGGGA GCGCCGCAGC 
CTTCGCGACC TGAAGCTCTG GTTCCTGGAG GAGGAGGGCG AGGAGGTCAG CACGTCCGTC 
ACGCGTCTCA TTCCTCAGGG AGAGTCTCTG AAGATGAGCA TCGCCTCCAT CTGCCTGACG 
CTGGAGGCCG GAGTGGGACA CCGCACCGTC CCCATGCTGC TGGCCAAAGC CTCGTTCCAG 
GGGGACGTCA AGGACTGGAG CACTCTCATC AACCTGCACT GCCACCTCAG CATGGAGGTG 
AACTACTATA ATGAGATGAT GGGTTTATGG GAGCCGCTGT TGGAGCCGCT GGACGATGAG 
GATAAAGACG GTTTTAGACC CTGGACTCTC GCACTGAAGA TGAAGAAGAA GCCGGTGGTC 
AGTTCTGGGG TGTCTGATGA GGTGGACGGC GCTGTGCCTG ATTATAAAAC GGTCATCAGC 
TTCACTAGTA AAGACCAGCT TAACGTCACC CTGTCCAAGT GTGGTCTGCA CATGCTCAGT 
AATCTGGGAA CGGCTTTTGC AGAGGCCGCT AAACAGACGG CCGAGTGCTT TCAGACAGAT 
CAGACACCAT TCGTGGTGAA GAATCGCCTG GGTTTGGCGG TGTCTGTGCT GCACAGCGAA 
GTGTTTCGAC CCATCGGGAA GGAGTCTAAG GATCGTGTGG TGGAGCTCCC GGATGGAGAG 
AGTCTGAATT TGGACTATAC CCTCACCGCC GAGTCCAGCG ACCAGTTCTC TGCCATGACC 
AGCCTCAGTG CCAAAAACTA CATACAGCCC ACTCCAGTCG GCCACACGAC CGCAAGTGTG 
ATCCCGCTCA TCAAAACGGG CCGGGGCATG TACAGCGTCA TGCACAACGA GTCCCGCGTC 
ACTCGCTTCT TCGTCTGTCA GATTTACACC GTCGAGGGCA GCAAATATGT CAAGATTCGC 
TCTCCCTTCC AGATTATCAA CCACTTTTCA GTGCCGTTCC GTGTTTATGA AGGATCCGTG 
TTTCTGGGCG AGTCTCTGCC GACAGAGGAG TTCTGTGTGC CGCTGGGATC CTACAGAGCT 
GAGCTGAGTC TGCAGCCTGT GCCTGATGGC GGTGAAGAGT ACGAGCACTC CGAGAGCTTC 
ACCTACACTG AGGTTGTCCA GAAACCCGGA AAGTGTCTGG AGAAAAACTG CCATCTGCGT 
GGTGACAGCG CCCAGATTCT GAAGGTCAAC ATGGTTCCTC TTGCGGACAC AGTGACGTCC 
CACAGCAGCG AGGAGGAGTT CGATAAGCCC ATCGTACTGC ATTTGTGGCC CACCGTCCTG 
CTGCGCAACC TGCTGCCCTA CCCAATTACT TTCAAGCTAA AGGGCATCGG AGATTCCCCC 
TCGGTGTCGC TGAAGGAGGG CCACTCATCC CAGATCCACA CCGCCATCAT GGACTTCTCC 
GTGTTGGATC TGAGGCTGCT GGGATACTTG CAGCAGGACT GGGAGTCTCA GTACAGCCTC 
AGCAGTGAAC AAGACGAGAT CAGCTTCATT AAGTTTCGCT CGCTCGCACG TGAGGAGGAG 
ACTGAGAGCC GGCCGGAGCT GGACATCGCT ATGCATGTGA AGAAGGAGGA GGGTCAGGTT 
GTGCTGGCCG TACACTCGCC GTACTGGATG GTGAATAAAA CAAGCCGACT GCTGCAGTAC 
AAGGCGGATG ATATTCATCG CAAACACCCC AAGGATTACG ACATGCCGCT GCTCTTCTCC 
TTCAAGCCAC GCAACTTCCT GCAAAACAAC AAGATCCGCC TGATGATCTC AGAAAGTGAG 
CTGTCAGATG ATTTCTCCAT CGACACTGTC GGGAGTTACG GAGATGTCAA ATGCAAAGGC 
AGACAGAAGG ATTACTTGGT GGGAGTGAAG ATCGATGCTA GCAGCTTCAG CTTGACACGT 
TTGGTGACTT TTCTGCCATT CTACATGCTG GTGAACAGAA CCAAACATGT GATTCGGGTC 
TGTGAGGAGG GGCAGGAGAA CTGGACAGAA GCCCGACCCG GACAGGCTGC TGTTCCATTC 
TGGCCAGAAA GAGACTCAAA GAAGCTGAGA GTGAAGGTGG AGGGCAGTCA GTCGTCACCA 
CGCGCCTTTG ACTTCCACCA GCCAGAGAAC TGCCTCCTGC TGCATCTGGA CAAAAGCTTG 
GGTGGCATCA TTGTGGATGT GAATCTGACA GAGCATTCAG CTGTGATCCT CTTCTCTGAT 
TATCATGATG GAGCCGCTCC CTTCCTCCTC ATCAACCACA CTAAGGACGA GACGCTGCAG 
TACCACCAGA GCTCTCAGGC CGAGATCGAT GAACTGAGTC CAGGTCAGGC GGTTTACTAC 
ACCTGGGCTG AACCAACCGG CTCCAGGATC CTCAGCTGGA AACTGGGCAA ATACAAAGGA 
GAGCTCAAGA GTGAAGAGGA TCAGTTGCTG GATGTGGACA AGGAGGGCAA GTTGTTTTTG 
GTGTCGTTTT ATGAAGGCCT CCAGCGTGTG GCGCTCTTCA CCAGCGAGCA GCGCATCTTC 
AAGAAGATCT GCGACAGTGA GAAGATAGAG CTGGCTCAGC AAGAAATCAG CATCTTGCTG 
CAGAACATGG GCATTTCGCT GGTCAACAAC AACTCCAGCC AGGAAGTGGC CTTCATTGGC 
ATCACCAGCT CTGATGTTGT GTGGGAAACC AAGCCGAAGA AGCGCAGCCG CTGGAAAGTT 
CTGAGTGTTA AAGAGGCAGC AGTTCTGGAG CAGCACTACA GAGACTACAT GGAGAACGGC 
CCAGTGGACA GCACCATCAT AGACCTGGAC AACGACTACC AGGTGTTGTT TGCTGCTAAC 
GGTGTGGACA TGAGGATCAT GCAGCCCTAC GATGCTCCAC TCAGAAGGAA CTTCCTGCCG 
GCGTTGAAGG TGGAGTACAG CGTTTCAGCC CGCCAGAAAA CATACAGAGT CCAGATCAAC 
AGGATCCAGA TCCAAAACCA GCTGCAAGGA GCCATATTCC CGTTTGTGTT TTACCCCATC 
AAACCGCCAA AGTCTGTCAG CATGGACTCA GAGCCAAAAC CGCTGGCTGA CGTGAGCATC 
ATCACCAGAG CAGCAGGACA CTCCGACATT CTGCGCATCA AATATTTTAT GGTGTTGATT 
CAGGAGATGG ACCTGCGGCT GGATCTGGGC TTTCTGTATG CCATACTTGA GCTCTTCACT 
GCTGAACACA CCAGTGCTGT GACGTCTGAA CAGGAGGTGG AGTTGTTTGA TAAAGACATA 
GAGTATCTAA AGACTGAGTT AAATCATGTT TCAGCTACAG ACACATCTCC CATCAGCCTC 
TACGAGTACT TCCACATCTC TCCTATAAAG CTTCACTTGA GCTTGTCTCT GAGCAGTGGT 
GGTGAAGACA GCAATAAGCA AGAGCGCGAG ACGGAGCTGA TCCCAGTTCA GTCTCTCCAT 
CTGCTGCTCA AGAGCATCGG GGCTACACTT ACAGACGCAC AGGACGTCCT CTTTAAACTG 
GGCTTCTTCG AGCTGAGGTA TCAGTTTCGC ACCACTCAGC AGCTGCAGTG GGAGGTCGTT 
CGCCATTACT CCAAACAGGC TATCAAGCAG ATGTATGTCC TTGTTCTGGG TTTGGACGTT 
CTTGGGAATC CATTCGGGCT GATCCGGGGC CTGTCTGAAG GCGTCGAGGC TTTCTTTTAC 
GAGCCCTATC AGGGTGCAAT TCAGGGTCCA GAGGAGTTCA TGGAGGGCAT GGCTCTCGGC 
GTGAAGGCTC TGGTTGGAGG CGCTGTGGGT GGTCTGGCCG GAGCGGCGTC TCGCATCACT 
GGAGCGATGG CTAAAGGTGT GGCGGCGATG ACGATGGATG AAGAGTACCA GCAGAAGAGA 
CGAGAGGCCA TGAACAAACA GCCCAGCGGA CTGAGAGAAG GACTGACACG AGGAGGAAAA 
GGACTGGTGT CTGGCTTCGT GAGTGGCATC ACGGGCATTG TGACCAAACC CATTAAAGGA 
GCTCAGAAGG AAGGAGCGGC AGGTTTCTTC AAGGGTATGG GTAAGGGTTT AGTTGGAGCT 
CTGACCAGAC CAACAGGGGG CATCATTGAC ATGGCCAGCA GCACCTTCCA GGGCATCAAG 
AGAGCTGCAG AGCTGTCTCA GGATGTGGAG AGTCTGCGCC CTCCACGCTT CATCCACGAG 
GACGGAGTGA TCCGGCCGTA CAGAGAGAGA GAAGGCCTCG GCAGTCAGAT GCTGCAGAGT 
ATTGAAAACG GACGGTTTGC CAAGTACAGG TACTTTGCTC ACGCTAAAGT CAACGAGTCT 
GATTTCCTGA TGATCACTAA AAGGGGGATC TTTTTCGTGA CAAAGGGCAC GTTTGGTCAG 
TTGACATGTG AATGGCAGTA TCTGTTCGAT GAGTTTACTA AAGCACCCAT GATTGTGGAA 
GGCAGGAGAC TGCGCATCGA AGCGAAGGAG AGAGTCAAGT CAGTGTTTCA CGCCAAAGAG 
TTCGGGAAGA TCATCAATTT CAAAACTCCA GAATTAGCAA AGTGGGTTCT TGCCAAGCTT 
GAAGACGCTC GGGAGAATCT GCCCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302584.1
CDS	51..9680
Translation	MVFESVVVDVLNRFLGDYVGNLDSSQLSLGIWGGDAILRNLEIKENALSQLDIPFKVRAGHIGRLELKIPWKNLYTQSVEATLDEVYLLIVPTASIKYDAEKEERQLQESRQRELQRIEETKQKAAEKENPKGEKQDTFVEKLVTQVIKNLQVKISNIHVRYEDDVTNPSVPLSFGISLQNLSLQTADQNWNPCLLDEKAKLFFKLVRLDNLFAYWNVSSELYSNHNPEEALRYLKHSVAIQTAIPQDYHFIFRPISANAKLRMNPRSDVDFSSPKMDLVVNLPEVAVELSRPQYVSILELLGSVDMMTRNLPYRKYRPYVVVHTNARQWWRYVITGVLEVNVKPRLHMWSWLHIRRHRQHVKKYRELYKNKIISKKPAESLLKELEETERTLDIFNITMARQQAEMEASKAGLRIYRPGVKVEEEQSQGWFGWMWNWSGEASATQTKDVKTGGFDGLMTPAEKAKLYAAIGYSETAVNPNLPKNFENMKMNFHLLRLSLSIKESREKAEIIKLSVIDLKAMLTQRPAAQAIKISAQLSSFEVSGLPREKSAPILLSPRHVDGMKDKSLLNLMFETNPLDARADQRLRIESQPLEIIYDAMTVNSMSAFFMPPKDLQLDELTNATLMKLEQFRDKTSTGLMYIIETQKVLDLKINLMASYVIVPENGFYDPKHNLAILDLGHFQMSSQSRADLPQLSAGSSTLEDIMSRAYDSFDVQLSSLQFLYSKAGGDWKRARPLRQSAFHILEPVDLKVVFSRAMVVTDSRMPKFKMHGELPLLSLRISDDKVRGVLALLSSIPLPESRPTPQSRNTQATPKASKRGNTPQLTPRRPAIDDLRASIIYGSEEELFFDAPSSPIEDLPFFEASSDSRHASAADKRLSMVLKDPKKNMTEFLLSFEINKLSVQLCRVSNRQEVSVLHLFIDGLGTELKLRTFDMSSYTFLREICLECSEYLDSEDKKVHLITTLDNTEEDLLTLEYVKADKNAPDFKAMYKNTEQLIKVNFSSLDIHLHTEALLNSMNFLNNLLPPMKKETQEEQPIIPKEEEEEEEEEEEEEEAKVENSAVAKKSSYKKSKFEDVVNLHIRADLNCLKVFIRGEKARISEISIEGLVSEVQMKKKSVEILANLKNIIILDCDKEALYKKAVSIADKEVFDFRMVNHVDATEGDAYTDMHIVDTSVTLTVGCIQVIFLNKFMSSILAFINNFQEAKNALAEVTAQAAEKAATGVLELAERSTRISLDVDIKAPVVFLPQSSVSHNVIVADLGLVTVKNRFEIVSSPTHTKIPPVMDIMAVGLSDLKMYRTTFTDDKFNSEIQLLKPVNMDLSIKRNLSASWYHSIPDIQINAHLKPMSLILSQEDLTVVLKTLSENLSETSDAPPAPSSSDKGDTVSNKGSQGSGGNTVVTAAVVEGQKSGKLKNTLKLNFKFDSMTLVLYSPNSEIQLSDSRDESLKLAEFTLGTISTSVHMFSDSSMKASLKLTNCLLDDKRQGVKMITSRMMAMRQDREDSVMVDVSYRQSRDGTRLDTLVQDLYLCASMEFLLTVADVFLKALSDGFSGPPKSNKQPASTSKDPAAPAPPVVAKTEMNVVVKNPEIVFVADLTRAEAPALVMTTQCELVMKSEPSGQCMTAAISDLKIVACPFLREKRENNVTTVLQPCEVFFQSRQSNTEPQCIELSISALTLKVSPIIINTVITIQTALSPAAETPQELESPIPQDLWERRSLRDLKLWFLEEEGEEVSTSVTRLIPQGESLKMSIASICLTLEAGVGHRTVPMLLAKASFQGDVKDWSTLINLHCHLSMEVNYYNEMMGLWEPLLEPLDDEDKDGFRPWTLALKMKKKPVVSSGVSDEVDGAVPDYKTVISFTSKDQLNVTLSKCGLHMLSNLGTAFAEAAKQTAECFQTDQTPFVVKNRLGLAVSVLHSEVFRPIGKESKDRVVELPDGESLNLDYTLTAESSDQFSAMTSLSAKNYIQPTPVGHTTASVIPLIKTGRGMYSVMHNESRVTRFFVCQIYTVEGSKYVKIRSPFQIINHFSVPFRVYEGSVFLGESLPTEEFCVPLGSYRAELSLQPVPDGGEEYEHSESFTYTEVVQKPGKCLEKNCHLRGDSAQILKVNMVPLADTVTSHSSEEEFDKPIVLHLWPTVLLRNLLPYPITFKLKGIGDSPSVSLKEGHSSQIHTAIMDFSVLDLRLLGYLQQDWESQYSLSSEQDEISFIKFRSLAREEETESRPELDIAMHVKKEEGQVVLAVHSPYWMVNKTSRLLQYKADDIHRKHPKDYDMPLLFSFKPRNFLQNNKIRLMISESELSDDFSIDTVGSYGDVKCKGRQKDYLVGVKIDASSFSLTRLVTFLPFYMLVNRTKHVIRVCEEGQENWTEARPGQAAVPFWPERDSKKLRVKVEGSQSSPRAFDFHQPENCLLLHLDKSLGGIIVDVNLTEHSAVILFSDYHDGAAPFLLINHTKDETLQYHQSSQAEIDELSPGQAVYYTWAEPTGSRILSWKLGKYKGELKSEEDQLLDVDKEGKLFLVSFYEGLQRVALFTSEQRIFKKICDSEKIELAQQEISILLQNMGISLVNNNSSQEVAFIGITSSDVVWETKPKKRSRWKVLSVKEAAVLEQHYRDYMENGPVDSTIIDLDNDYQVLFAANGVDMRIMQPYDAPLRRNFLPALKVEYSVSARQKTYRVQINRIQIQNQLQGAIFPFVFYPIKPPKSVSMDSEPKPLADVSIITRAAGHSDILRIKYFMVLIQEMDLRLDLGFLYAILELFTAEHTSAVTSEQEVELFDKDIEYLKTELNHVSATDTSPISLYEYFHISPIKLHLSLSLSSGGEDSNKQERETELIPVQSLHLLLKSIGATLTDAQDVLFKLGFFELRYQFRTTQQLQWEVVRHYSKQAIKQMYVLVLGLDVLGNPFGLIRGLSEGVEAFFYEPYQGAIQGPEEFMEGMALGVKALVGGAVGGLAGAASRITGAMAKGVAAMTMDEEYQQKRREAMNKQPSGLREGLTRGGKGLVSGFVSGITGIVTKPIKGAQKEGAAGFFKGMGKGLVGALTRPTGGIIDMASSTFQGIKRAAELSQDVESLRPPRFIHEDGVIRPYREREGLGSQMLQSIENGRFAKYRYFAHAKVNESDFLMITKRGIFFVTKGTFGQLTCEWQYLFDEFTKAPMIVEGRRLRIEAKERVKSVFHAKEFGKIINFKTPELAKWVLAKLEDARENLPK

Target ORF information:

RefSeq Version	XM_009304309.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio vacuolar protein sorting 13 homolog A (S. cerevisiae) (vps13a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304309.3

ORF Insert Sequence:

ATGGTGTTTG AGTCGGTGGT GGTCGACGTG CTGAACCGGT TTCTGGGGGA CTATGTTGGA 
AACCTCGACA GCTCGCAGCT CAGCCTCGGC ATATGGGGAG GTGATGCCAT ATTGAGGAAC 
CTCGAGATTA AAGAAAATGC ATTGAGCCAG CTGGACATTC CTTTCAAAGT CAGAGCAGGA 
CACATTGGGC GTTTGGAGTT AAAGATCCCC TGGAAGAACC TGTACACTCA GTCTGTGGAG 
GCCACGCTGG ATGAAGTTTA CCTGCTCATC GTACCTACTG CTAGTATAAA GTATGACGCA 
GAGAAGGAGG AGCGGCAGCT GCAGGAGTCT CGGCAGAGGG AGCTCCAGCG CATCGAGGAG 
ACCAAACAGA AAGCAGCAGA AAAAGAGAAT CCAAAGGGTG AGAAGCAGGA CACATTTGTG 
GAGAAACTGG TAACACAGGT GATCAAAAAC CTGCAGGTCA AAATATCCAA CATTCACGTC 
CGCTATGAGG ATGATGTCAC CAATCCCAGT GTTCCTCTCT CCTTTGGGAT ATCATTACAA 
AACCTCAGTC TCCAGACGGC GGATCAGAAC TGGAACCCCT GTCTTCTGGA TGAGAAAGCC 
AAACTGTTTT TTAAGCTTGT ACGTCTGGAT AATCTGTTTG CGTACTGGAA CGTCAGTTCA 
GAGCTGTACT CCAACCACAA CCCTGAGGAG GCTCTGCGAT ACCTGAAGCA CAGCGTAGCC 
ATCCAGACTG CCATCCCTCA GGATTATCAC TTCATCTTTC GTCCAATATC TGCCAATGCC 
AAGTTGCGGA TGAACCCTCG CTCTGATGTA GACTTCTCCT CACCCAAAAT GGACCTGGTG 
GTCAACCTTC CTGAAGTGGC CGTGGAGCTC AGCCGACCAC AGTACGTCAG CATTCTGGAG 
CTGTTGGGGT CGGTGGATAT GATGACCCGT AACCTTCCTT ACAGGAAGTA TCGGCCATAT 
GTGGTGGTTC ACACTAACGC TCGTCAGTGG TGGCGATATG TGATCACGGG TGTTCTGGAG 
GTCAATGTGA AGCCTCGTCT TCACATGTGG TCTTGGCTGC ATATTCGTCG ACATCGGCAG 
CATGTGAAAA AGTACAGAGA GCTTTATAAG AATAAAATCA TCAGTAAGAA ACCGGCAGAG 
AGTCTGCTGA AGGAGCTGGA GGAGACTGAA AGAACATTAG ACATATTCAA CATCACAATG 
GCGAGACAGC AGGCTGAGAT GGAGGCCTCG AAGGCAGGGC TCAGGATTTA CCGGCCCGGG 
GTGAAGGTGG AGGAGGAACA GTCACAGGGC TGGTTTGGCT GGATGTGGAA CTGGTCTGGA 
GAAGCCAGTG CCACTCAAAC CAAAGACGTC AAAACTGGAG GTTTTGATGG GTTAATGACG 
CCTGCAGAGA AAGCCAAACT ATACGCTGCC ATCGGATACA GCGAAACTGC TGTCAACCCC 
AACCTGCCAA AAAATTTTGA AAACATGAAG ATGAATTTCC ATCTTCTCCG CCTGTCCCTG 
TCCATCAAAG AGAGCCGAGA GAAGGCGGAG ATCATCAAAC TGTCTGTCAT TGACCTGAAG 
GCCATGCTGA CTCAAAGACC CGCGGCTCAA GCTATAAAGA TCTCCGCTCA GCTGTCGTCG 
TTTGAAGTGT CCGGTCTCCC GCGGGAAAAG TCGGCCCCGA TCCTGCTTTC TCCACGTCAT 
GTGGACGGCA TGAAGGATAA AAGTCTGCTG AACCTCATGT TCGAGACCAA CCCGCTGGAC 
GCTCGTGCTG ACCAGAGACT GCGCATCGAG TCCCAGCCGC TGGAGATCAT CTATGATGCA 
ATGACCGTGA ACAGTATGTC CGCTTTCTTC ATGCCTCCTA AAGACCTGCA GCTGGATGAA 
CTGACCAACG CCACACTGAT GAAGCTGGAG CAGTTCAGAG ACAAAACCTC CACAGGCCTC 
ATGTACATCA TTGAGACGCA GAAAGTTCTG GATCTGAAAA TAAACCTCAT GGCCTCGTAT 
GTCATCGTAC CGGAAAACGG CTTTTATGAC CCCAAACACA ACCTTGCCAT TCTAGATCTG 
GGTCATTTTC AGATGTCAAG TCAGAGCCGA GCTGATCTTC CTCAGCTGTC TGCGGGCAGC 
AGTACTCTAG AGGACATCAT GTCTCGAGCG TACGACAGCT TCGACGTCCA GCTCAGCAGT 
CTGCAGTTCC TCTACTCCAA AGCAGGTGGA GACTGGAAGC GGGCTCGTCC GCTAAGACAG 
TCTGCTTTCC ACATCCTGGA GCCAGTGGAC CTGAAGGTGG TCTTTAGCAG AGCGATGGTG 
GTCACAGACT CCAGGATGCC CAAGTTTAAG ATGCACGGTG AGCTGCCTCT GCTGTCTCTG 
CGGATCTCTG ATGATAAAGT GCGTGGCGTT CTGGCTCTGC TCAGCAGTAT TCCTCTACCT 
GAGAGCCGGC CCACGCCTCA GTCCAGAAAC ACGCAGGCCA CTCCAAAGGC CTCCAAGCGA 
GGAAATACCC CTCAGCTCAC GCCCCGAAGG CCAGCTATAG ATGACCTGCG TGCGTCCATC 
ATATACGGCT CAGAGGAGGA GCTGTTCTTT GACGCCCCCA GTTCACCGAT CGAGGATCTG 
CCGTTCTTCG AGGCCTCGTC GGACTCTCGG CATGCTTCAG CAGCTGATAA ACGTCTGTCT 
ATGGTGCTCA AAGACCCCAA GAAGAACATG ACTGAGTTTC TGCTTAGTTT TGAGATCAAC 
AAGCTCTCGG TGCAGCTGTG CCGTGTGTCG AATAGACAGG AAGTGAGTGT TCTGCATCTG 
TTCATCGATG GATTGGGAAC AGAGCTCAAA CTTCGCACGT TTGACATGTC GTCGTACACT 
TTCCTGCGTG AGATCTGCCT CGAGTGCTCA GAATACTTGG ATTCTGAGGA CAAGAAAGTT 
CACCTTATCA CCACGCTGGA CAACACAGAG GAGGATCTGC TCACTCTGGA GTATGTCAAG 
GCAGACAAAA ATGCACCAGA TTTTAAAGCT ATGTACAAAA ATACAGAGCA ACTAATCAAG 
GTGAATTTCT CCTCTCTGGA CATTCACCTG CACACAGAGG CTCTCCTGAA CTCCATGAAC 
TTCCTAAATA ATCTTCTGCC ACCGATGAAG AAGGAGACCC AGGAGGAACA ACCAATAATT 
CCCAAGGAGG AAGAGGAGGA GGAGGAGGAA GAAGAAGAAG AGGAGGAGGC TAAAGTAGAG 
AATTCTGCTG TGGCCAAGAA GTCTTCCTAC AAGAAGTCAA AGTTTGAGGA TGTGGTGAAT 
TTGCACATCC GCGCAGACCT CAACTGTCTG AAGGTGTTCA TACGAGGAGA GAAAGCCAGG 
ATCTCTGAGA TCAGCATAGA GGGACTGGTT TCTGAAGTTC AGATGAAGAA GAAGTCTGTG 
GAGATTCTGG CTAACTTGAA GAACATCATC ATTCTGGATT GTGACAAAGA GGCACTTTAT 
AAAAAGGCAG TGTCCATCGC TGATAAGGAG GTGTTTGATT TCCGCATGGT GAACCATGTC 
GATGCAACGG AGGGTGACGC TTACACAGAC ATGCACATTG TGGACACTAG CGTAACCCTG 
ACCGTGGGCT GCATTCAAGT TATTTTCCTC AACAAGTTTA TGTCCTCAAT ACTGGCATTT 
ATTAATAATT TCCAGGAGGC TAAAAATGCA TTGGCGGAGG TGACGGCCCA GGCAGCAGAG 
AAAGCAGCCA CGGGGGTTCT GGAGCTGGCA GAGAGAAGTA CTCGGATCTC CCTGGACGTC 
GACATCAAAG CTCCTGTTGT CTTCCTCCCA CAATCCTCAG TTTCCCACAA CGTCATTGTG 
GCAGATCTGG GTTTAGTGAC TGTAAAGAAC CGCTTTGAGA TCGTCTCCTC TCCAACACAC 
ACCAAGATCC CTCCTGTCAT GGACATCATG GCTGTCGGAT TGAGTGATCT GAAAATGTAC 
AGGACAACGT TCACTGATGA CAAGTTTAAC AGCGAGATTC AGTTGCTCAA GCCAGTCAAC 
ATGGACCTGT CAATCAAACG CAATCTCTCC GCCTCCTGGT ATCACAGCAT ACCCGACATC 
CAGATCAATG CTCACCTCAA GCCCATGAGC CTGATCTTGA GTCAGGAGGA TCTGACTGTG 
GTGCTGAAGA CTCTGAGTGA GAATCTGTCC GAGACGTCTG ATGCTCCTCC AGCTCCGTCC 
AGCTCTGATA AAGGAGATAC GGTTTCTAAC AAAGGCTCCC AGGGTTCTGG AGGCAACACC 
GTTGTGACCG CAGCAGTGGT GGAGGGTCAG AAATCAGGCA AACTGAAGAA CACCCTAAAA 
CTGAACTTCA AGTTTGACTC CATGACGCTG GTCCTCTACA GCCCGAACAG CGAGATCCAG 
TTGTCCGACT CTCGTGATGA GAGTTTGAAG TTGGCCGAGT TCACACTGGG CACCATCTCC 
ACCAGTGTGC ACATGTTCTC CGACAGCTCC ATGAAGGCCT CGCTCAAACT CACCAACTGT 
CTGCTGGATG ACAAGAGGCA GGGAGTGAAG ATGATTACAT CCAGGATGAT GGCGATGCGT 
CAGGACCGAG AGGACAGTGT GATGGTGGAC GTCAGCTACC GCCAGAGTCG GGACGGGACT 
CGTCTGGACA CCCTCGTGCA AGACCTTTAT CTTTGTGCCA GTATGGAGTT TCTGCTCACT 
GTAGCTGATG TGTTTCTTAA AGCTCTGTCT GACGGCTTCT CCGGTCCTCC AAAGAGTAAC 
AAACAACCGG CCAGCACCTC TAAAGACCCA GCGGCTCCTG CTCCACCAGT GGTTGCTAAA 
ACCGAGATGA ATGTGGTGGT GAAGAACCCA GAGATCGTGT TTGTGGCGGA TCTGACGCGT 
GCTGAAGCTC CGGCTCTGGT CATGACCACT CAGTGTGAGC TGGTGATGAA GAGCGAGCCC 
AGCGGTCAGT GCATGACGGC CGCCATTTCA GACCTGAAGA TTGTGGCCTG TCCGTTCCTG 
CGGGAGAAGA GGGAGAACAA CGTGACGACG GTCCTACAGC CCTGCGAGGT TTTCTTCCAG 
AGCCGACAGT CAAACACTGA GCCGCAGTGC ATCGAGCTGT CCATCAGCGC ACTCACGCTC 
AAGGTGTCTC CGATCATCAT AAACACGGTC ATCACCATTC AGACGGCTCT AAGTCCTGCC 
GCTGAGACGC CGCAGGAGCT GGAGAGCCCC ATCCCACAGG ACCTATGGGA GCGCCGCAGC 
CTTCGCGACC TGAAGCTCTG GTTCCTGGAG GAGGAGGGCG AGGAGGTCAG CACGTCCGTC 
ACGCGTCTCA TTCCTCAGGG AGAGTCTCTG AAGATGAGCA TCGCCTCCAT CTGCCTGACG 
CTGGAGGCCG GAGTGGGACA CCGCACCGTC CCCATGCTGC TGGCCAAAGC CTCGTTCCAG 
GGGGACGTCA AGGACTGGAG CACTCTCATC AACCTGCACT GCCACCTCAG CATGGAGGTG 
AACTACTATA ATGAGATGAT GGGTTTATGG GAGCCGCTGT TGGAGCCGCT GGACGATGAG 
GATAAAGACG GTTTTAGACC CTGGACTCTC GCACTGAAGA TGAAGAAGAA GCCGGTGGTC 
AGTTCTGGGG TGTCTGATGA GGTGGACGGC GCTGTGCCTG ATTATAAAAC GGTCATCAGC 
TTCACTAGTA AAGACCAGCT TAACGTCACC CTGTCCAAGT GTGGTCTGCA CATGCTCAGT 
AATCTGGGAA CGGCTTTTGC AGAGGCCGCT AAACAGACGG CCGAGTGCTT TCAGACAGAT 
CAGACACCAT TCGTGGTGAA GAATCGCCTG GGTTTGGCGG TGTCTGTGCT GCACAGCGAA 
GTGTTTCGAC CCATCGGGAA GGAGTCTAAG GATCGTGTGG TGGAGCTCCC GGATGGAGAG 
AGTCTGAATT TGGACTATAC CCTCACCGCC GAGTCCAGCG ACCAGTTCTC TGCCATGACC 
AGCCTCAGTG CCAAAAACTA CATACAGCCC ACTCCAGTCG GCCACACGAC CGCAAGTGTG 
ATCCCGCTCA TCAAAACGGG CCGGGGCATG TACAGCGTCA TGCACAACGA GTCCCGCGTC 
ACTCGCTTCT TCGTCTGTCA GATTTACACC GTCGAGGGCA GCAAATATGT CAAGATTCGC 
TCTCCCTTCC AGATTATCAA CCACTTTTCA GTGCCGTTCC GTGTTTATGA AGGATCCGTG 
TTTCTGGGCG AGTCTCTGCC GACAGAGGAG TTCTGTGTGC CGCTGGGATC CTACAGAGCT 
GAGCTGAGTC TGCAGCCTGT GCCTGATGGC GGTGAAGAGT ACGAGCACTC CGAGAGCTTC 
ACCTACACTG AGGTTGTCCA GAAACCCGGA AAGTGTCTGG AGAAAAACTG CCATCTGCGT 
GGTGACAGCG CCCAGATTCT GAAGGTCAAC ATGGTTCCTC TTGCGGACAC AGTGACGTCC 
CACAGCAGCG AGGAGGAGTT CGATAAGCCC ATCGTACTGC ATTTGTGGCC CACCGTCCTG 
CTGCGCAACC TGCTGCCCTA CCCAATTACT TTCAAGCTAA AGGGCATCGG AGATTCCCCC 
TCGGTGTCGC TGAAGGAGGG CCACTCATCC CAGATCCACA CCGCCATCAT GGACTTCTCC 
GTGTTGGATC TGAGGCTGCT GGGATACTTG CAGCAGGACT GGGAGTCTCA GTACAGCCTC 
AGCAGTGAAC AAGACGAGAT CAGCTTCATT AAGTTTCGCT CGCTCGCACG TGAGGAGGAG 
ACTGAGAGCC GGCCGGAGCT GGACATCGCT ATGCATGTGA AGAAGGAGGA GGGTCAGGTT 
GTGCTGGCCG TACACTCGCC GTACTGGATG GTGAATAAAA CAAGCCGACT GCTGCAGTAC 
AAGGCGGATG ATATTCATCG CAAACACCCC AAGGATTACG ACATGCCGCT GCTCTTCTCC 
TTCAAGCCAC GCAACTTCCT GCAAAACAAC AAGATCCGCC TGATGATCTC AGAAAGTGAG 
CTGTCAGATG ATTTCTCCAT CGACACTGTC GGGAGTTACG GAGATGTCAA ATGCAAAGGC 
AGACAGAAGG ATTACTTGGT GGGAGTGAAG ATCGATGCTA GCAGCTTCAG CTTGACACGT 
TTGGTGACTT TTCTGCCATT CTACATGCTG GTGAACAGAA CCAAACATGT GATTCGGGTC 
TGTGAGGAGG GGCAGGAGAA CTGGACAGAA GCCCGACCCG GACAGGCTGC TGTTCCATTC 
TGGCCAGAAA GAGACTCAAA GAAGCTGAGA GTGAAGGTGG AGGGCAGTCA GTCGTCACCA 
CGCGCCTTTG ACTTCCACCA GCCAGAGAAC TGCCTCCTGC TGCATCTGGA CAAAAGCTTG 
GGTGGCATCA TTGTGGATGT GAATCTGACA GAGCATTCAG CTGTGATCCT CTTCTCTGAT 
TATCATGATG GAGCCGCTCC CTTCCTCCTC ATCAACCACA CTAAGGACGA GACGCTGCAG 
TACCACCAGA GCTCTCAGGC CGAGATCGAT GAACTGAGTC CAGGTCAGGC GGTTTACTAC 
ACCTGGGCTG AACCAACCGG CTCCAGGATC CTCAGCTGGA AACTGGGCAA ATACAAAGGA 
GAGCTCAAGA GTGAAGAGGA TCAGTTGCTG GATGTGGACA AGGAGGGCAA GTTGTTTTTG 
GTGTCGTTTT ATGAAGGCCT CCAGCGTGTG GCGCTCTTCA CCAGCGAGCA GCGCATCTTC 
AAGAAGATCT GCGACAGTGA GAAGATAGAG CTGGCTCAGC AAGAAATCAG CATCTTGCTG 
CAGAACATGG GCATTTCGCT GGTCAACAAC AACTCCAGCC AGGAAGTGGC CTTCATTGGC 
ATCACCAGCT CTGATGTTGT GTGGGAAACC AAGCCGAAGA AGCGCAGCCG CTGGAAAGTT 
CTGAGTGTTA AAGAGGCAGC AGTTCTGGAG CAGCACTACA GAGACTACAT GGAGAACGGC 
CCAGTGGACA GCACCATCAT AGACCTGGAC AACGACTACC AGGTGTTGTT TGCTGCTAAC 
GGTGTGGACA TGAGGATCAT GCAGCCCTAC GATGCTCCAC TCAGAAGGAA CTTCCTGCCG 
GCGTTGAAGG TGGAGTACAG CGTTTCAGCC CGCCAGAAAA CATACAGAGT CCAGATCAAC 
AGGATCCAGA TCCAAAACCA GCTGCAAGGA GCCATATTCC CGTTTGTGTT TTACCCCATC 
AAACCGCCAA AGTCTGTCAG CATGGACTCA GAGCCAAAAC CGCTGGCTGA CGTGAGCATC 
ATCACCAGAG CAGCAGGACA CTCCGACATT CTGCGCATCA AATATTTTAT GGTGTTGATT 
CAGGAGATGG ACCTGCGGCT GGATCTGGGC TTTCTGTATG CCATACTTGA GCTCTTCACT 
GCTGAACACA CCAGTGCTGT GACGTCTGAA CAGGAGGTGG AGTTGTTTGA TAAAGACATA 
GAGTATCTAA AGACTGAGTT AAATCATGTT TCAGCTACAG ACACATCTCC CATCAGCCTC 
TACGAGTACT TCCACATCTC TCCTATAAAG CTTCACTTGA GCTTGTCTCT GAGCAGTGGT 
GGTGAAGACA GCAATAAGCA AGAGCGCGAG ACGGAGCTGA TCCCAGTTCA GTCTCTCCAT 
CTGCTGCTCA AGAGCATCGG GGCTACACTT ACAGACGCAC AGGACGTCCT CTTTAAACTG 
GGCTTCTTCG AGCTGAGGTA TCAGTTTCGC ACCACTCAGC AGCTGCAGTG GGAGGTCGTT 
CGCCATTACT CCAAACAGGC TATCAAGCAG ATGTATGTCC TTGTTCTGGG TTTGGACGTT 
CTTGGGAATC CATTCGGGCT GATCCGGGGC CTGTCTGAAG GCGTCGAGGC TTTCTTTTAC 
GAGCCCTATC AGGGTGCAAT TCAGGGTCCA GAGGAGTTCA TGGAGGGCAT GGCTCTCGGC 
GTGAAGGCTC TGGTTGGAGG CGCTGTGGGT GGTCTGGCCG GAGCGGCGTC TCGCATCACT 
GGAGCGATGG CTAAAGGTGT GGCGGCGATG ACGATGGATG AAGAGTACCA GCAGAAGAGA 
CGAGAGGCCA TGAACAAACA GCCCAGCGGA CTGAGAGAAG GACTGACACG AGGAGGAAAA 
GGACTGGTGT CTGGCTTCGT GAGTGGCATC ACGGGCATTG TGACCAAACC CATTAAAGGA 
GCTCAGAAGG AAGGAGCGGC AGGTTTCTTC AAGGGTATGG GTAAGGGTTT AGTTGGAGCT 
CTGACCAGAC CAACAGGGGG CATCATTGAC ATGGCCAGCA GCACCTTCCA GGGCATCAAG 
AGAGCTGCAG AGCTGTCTCA GGATGTGGAG AGTCTGCGCC CTCCACGCTT CATCCACGAG 
GACGGAGTGA TCCGGCCGTA CAGAGAGAGA GAAGGCCTCG GCAGTCAGAT GCTGCAGAGT 
ATTGAAAACG GACGGTTTGC CAAGTACAGG TACTTTGCTC ACGCTAAAGT CAACGAGTCT 
GATTTCCTGA TGATCACTAA AAGGGGGATC TTTTTCGTGA CAAAGGGCAC GTTTGGTCAG 
TTGACATGTG AATGGCAGTA TCTGTTCGAT GAGTTTACTA AAGCACCCAT GATTGTGGAA 
GGCAGGAGAC TGCGCATCGA AGCGAAGGAG AGAGTCAAGT CAGTGTTTCA CGCCAAAGAG 
TTCGGGAAGA TCATCAATTT CAAAACTCCA GAATTAGCAA AGTGGGTTCT TGCCAAGCTT 
GAAGACGCTC GGGAGAATCT GCCCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS13A ( XM_009304309.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009302584.1 Danio rerio vacuolar protein sorting 13 homolog A (S. cerevisiae) (vps13a), transcript variant X2, mRNA.

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CloneID	ODa40321
Clone ID Related Accession (Same CDS sequence)	XM_009304309.3 , XM_009304309.2
Accession Version	XM_009304309.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	vacuolar protein sorting-associated protein 13A isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304309.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGTTTG AGTCGGTGGT GGTCGACGTG CTGAACCGGT TTCTGGGGGA CTATGTTGGA 
AACCTCGACA GCTCGCAGCT CAGCCTCGGC ATATGGGGAG GTGATGCCAT ATTGAGGAAC 
CTCGAGATTA AAGAAAATGC ATTGAGCCAG CTGGACATTC CTTTCAAAGT CAGAGCAGGA 
CACATTGGGC GTTTGGAGTT AAAGATCCCC TGGAAGAACC TGTACACTCA GTCTGTGGAG 
GCCACGCTGG ATGAAGTTTA CCTGCTCATC GTACCTACTG CTAGTATAAA GTATGACGCA 
GAGAAGGAGG AGCGGCAGCT GCAGGAGTCT CGGCAGAGGG AGCTCCAGCG CATCGAGGAG 
ACCAAACAGA AAGCAGCAGA AAAAGAGAAT CCAAAGGGTG AGAAGCAGGA CACATTTGTG 
GAGAAACTGG TAACACAGGT GATCAAAAAC CTGCAGGTCA AAATATCCAA CATTCACGTC 
CGCTATGAGG ATGATGTCAC CAATCCCAGT GTTCCTCTCT CCTTTGGGAT ATCATTACAA 
AACCTCAGTC TCCAGACGGC GGATCAGAAC TGGAACCCCT GTCTTCTGGA TGAGAAAGCC 
AAACTGTTTT TTAAGCTTGT ACGTCTGGAT AATCTGTTTG CGTACTGGAA CGTCAGTTCA 
GAGCTGTACT CCAACCACAA CCCTGAGGAG GCTCTGCGAT ACCTGAAGCA CAGCGTAGCC 
ATCCAGACTG CCATCCCTCA GGATTATCAC TTCATCTTTC GTCCAATATC TGCCAATGCC 
AAGTTGCGGA TGAACCCTCG CTCTGATGTA GACTTCTCCT CACCCAAAAT GGACCTGGTG 
GTCAACCTTC CTGAAGTGGC CGTGGAGCTC AGCCGACCAC AGTACGTCAG CATTCTGGAG 
CTGTTGGGGT CGGTGGATAT GATGACCCGT AACCTTCCTT ACAGGAAGTA TCGGCCATAT 
GTGGTGGTTC ACACTAACGC TCGTCAGTGG TGGCGATATG TGATCACGGG TGTTCTGGAG 
GTCAATGTGA AGCCTCGTCT TCACATGTGG TCTTGGCTGC ATATTCGTCG ACATCGGCAG 
CATGTGAAAA AGTACAGAGA GCTTTATAAG AATAAAATCA TCAGTAAGAA ACCGGCAGAG 
AGTCTGCTGA AGGAGCTGGA GGAGACTGAA AGAACATTAG ACATATTCAA CATCACAATG 
GCGAGACAGC AGGCTGAGAT GGAGGCCTCG AAGGCAGGGC TCAGGATTTA CCGGCCCGGG 
GTGAAGGTGG AGGAGGAACA GTCACAGGGC TGGTTTGGCT GGATGTGGAA CTGGTCTGGA 
GAAGCCAGTG CCACTCAAAC CAAAGACGTC AAAACTGGAG GTTTTGATGG GTTAATGACG 
CCTGCAGAGA AAGCCAAACT ATACGCTGCC ATCGGATACA GCGAAACTGC TGTCAACCCC 
AACCTGCCAA AAAATTTTGA AAACATGAAG ATGAATTTCC ATCTTCTCCG CCTGTCCCTG 
TCCATCAAAG AGAGCCGAGA GAAGGCGGAG ATCATCAAAC TGTCTGTCAT TGACCTGAAG 
GCCATGCTGA CTCAAAGACC CGCGGCTCAA GCTATAAAGA TCTCCGCTCA GCTGTCGTCG 
TTTGAAGTGT CCGGTCTCCC GCGGGAAAAG TCGGCCCCGA TCCTGCTTTC TCCACGTCAT 
GTGGACGGCA TGAAGGATAA AAGTCTGCTG AACCTCATGT TCGAGACCAA CCCGCTGGAC 
GCTCGTGCTG ACCAGAGACT GCGCATCGAG TCCCAGCCGC TGGAGATCAT CTATGATGCA 
ATGACCGTGA ACAGTATGTC CGCTTTCTTC ATGCCTCCTA AAGACCTGCA GCTGGATGAA 
CTGACCAACG CCACACTGAT GAAGCTGGAG CAGTTCAGAG ACAAAACCTC CACAGGCCTC 
ATGTACATCA TTGAGACGCA GAAAGTTCTG GATCTGAAAA TAAACCTCAT GGCCTCGTAT 
GTCATCGTAC CGGAAAACGG CTTTTATGAC CCCAAACACA ACCTTGCCAT TCTAGATCTG 
GGTCATTTTC AGATGTCAAG TCAGAGCCGA GCTGATCTTC CTCAGCTGTC TGCGGGCAGC 
AGTACTCTAG AGGACATCAT GTCTCGAGCG TACGACAGCT TCGACGTCCA GCTCAGCAGT 
CTGCAGTTCC TCTACTCCAA AGCAGGTGGA GACTGGAAGC GGGCTCGTCC GCTAAGACAG 
TCTGCTTTCC ACATCCTGGA GCCAGTGGAC CTGAAGGTGG TCTTTAGCAG AGCGATGGTG 
GTCACAGACT CCAGGATGCC CAAGTTTAAG ATGCACGGTG AGCTGCCTCT GCTGTCTCTG 
CGGATCTCTG ATGATAAAGT GCGTGGCGTT CTGGCTCTGC TCAGCAGTAT TCCTCTACCT 
GAGAGCCGGC CCACGCCTCA GTCCAGAAAC ACGCAGGCCA CTCCAAAGGC CTCCAAGCGA 
GGAAATACCC CTCAGCTCAC GCCCCGAAGG CCAGCTATAG ATGACCTGCG TGCGTCCATC 
ATATACGGCT CAGAGGAGGA GCTGTTCTTT GACGCCCCCA GTTCACCGAT CGAGGATCTG 
CCGTTCTTCG AGGCCTCGTC GGACTCTCGG CATGCTTCAG CAGCTGATAA ACGTCTGTCT 
ATGGTGCTCA AAGACCCCAA GAAGAACATG ACTGAGTTTC TGCTTAGTTT TGAGATCAAC 
AAGCTCTCGG TGCAGCTGTG CCGTGTGTCG AATAGACAGG AAGTGAGTGT TCTGCATCTG 
TTCATCGATG GATTGGGAAC AGAGCTCAAA CTTCGCACGT TTGACATGTC GTCGTACACT 
TTCCTGCGTG AGATCTGCCT CGAGTGCTCA GAATACTTGG ATTCTGAGGA CAAGAAAGTT 
CACCTTATCA CCACGCTGGA CAACACAGAG GAGGATCTGC TCACTCTGGA GTATGTCAAG 
GCAGACAAAA ATGCACCAGA TTTTAAAGCT ATGTACAAAA ATACAGAGCA ACTAATCAAG 
GTGAATTTCT CCTCTCTGGA CATTCACCTG CACACAGAGG CTCTCCTGAA CTCCATGAAC 
TTCCTAAATA ATCTTCTGCC ACCGATGAAG AAGGAGACCC AGGAGGAACA ACCAATAATT 
CCCAAGGAGG AAGAGGAGGA GGAGGAGGAA GAAGAAGAAG AGGAGGAGGC TAAAGTAGAG 
AATTCTGCTG TGGCCAAGAA GTCTTCCTAC AAGAAGTCAA AGTTTGAGGA TGTGGTGAAT 
TTGCACATCC GCGCAGACCT CAACTGTCTG AAGGTGTTCA TACGAGGAGA GAAAGCCAGG 
ATCTCTGAGA TCAGCATAGA GGGACTGGTT TCTGAAGTTC AGATGAAGAA GAAGTCTGTG 
GAGATTCTGG CTAACTTGAA GAACATCATC ATTCTGGATT GTGACAAAGA GGCACTTTAT 
AAAAAGGCAG TGTCCATCGC TGATAAGGAG GTGTTTGATT TCCGCATGGT GAACCATGTC 
GATGCAACGG AGGGTGACGC TTACACAGAC ATGCACATTG TGGACACTAG CGTAACCCTG 
ACCGTGGGCT GCATTCAAGT TATTTTCCTC AACAAGTTTA TGTCCTCAAT ACTGGCATTT 
ATTAATAATT TCCAGGAGGC TAAAAATGCA TTGGCGGAGG TGACGGCCCA GGCAGCAGAG 
AAAGCAGCCA CGGGGGTTCT GGAGCTGGCA GAGAGAAGTA CTCGGATCTC CCTGGACGTC 
GACATCAAAG CTCCTGTTGT CTTCCTCCCA CAATCCTCAG TTTCCCACAA CGTCATTGTG 
GCAGATCTGG GTTTAGTGAC TGTAAAGAAC CGCTTTGAGA TCGTCTCCTC TCCAACACAC 
ACCAAGATCC CTCCTGTCAT GGACATCATG GCTGTCGGAT TGAGTGATCT GAAAATGTAC 
AGGACAACGT TCACTGATGA CAAGTTTAAC AGCGAGATTC AGTTGCTCAA GCCAGTCAAC 
ATGGACCTGT CAATCAAACG CAATCTCTCC GCCTCCTGGT ATCACAGCAT ACCCGACATC 
CAGATCAATG CTCACCTCAA GCCCATGAGC CTGATCTTGA GTCAGGAGGA TCTGACTGTG 
GTGCTGAAGA CTCTGAGTGA GAATCTGTCC GAGACGTCTG ATGCTCCTCC AGCTCCGTCC 
AGCTCTGATA AAGGAGATAC GGTTTCTAAC AAAGGCTCCC AGGGTTCTGG AGGCAACACC 
GTTGTGACCG CAGCAGTGGT GGAGGGTCAG AAATCAGGCA AACTGAAGAA CACCCTAAAA 
CTGAACTTCA AGTTTGACTC CATGACGCTG GTCCTCTACA GCCCGAACAG CGAGATCCAG 
TTGTCCGACT CTCGTGATGA GAGTTTGAAG TTGGCCGAGT TCACACTGGG CACCATCTCC 
ACCAGTGTGC ACATGTTCTC CGACAGCTCC ATGAAGGCCT CGCTCAAACT CACCAACTGT 
CTGCTGGATG ACAAGAGGCA GGGAGTGAAG ATGATTACAT CCAGGATGAT GGCGATGCGT 
CAGGACCGAG AGGACAGTGT GATGGTGGAC GTCAGCTACC GCCAGAGTCG GGACGGGACT 
CGTCTGGACA CCCTCGTGCA AGACCTTTAT CTTTGTGCCA GTATGGAGTT TCTGCTCACT 
GTAGCTGATG TGTTTCTTAA AGCTCTGTCT GACGGCTTCT CCGGTCCTCC AAAGAGTAAC 
AAACAACCGG CCAGCACCTC TAAAGACCCA GCGGCTCCTG CTCCACCAGT GGTTGCTAAA 
ACCGAGATGA ATGTGGTGGT GAAGAACCCA GAGATCGTGT TTGTGGCGGA TCTGACGCGT 
GCTGAAGCTC CGGCTCTGGT CATGACCACT CAGTGTGAGC TGGTGATGAA GAGCGAGCCC 
AGCGGTCAGT GCATGACGGC CGCCATTTCA GACCTGAAGA TTGTGGCCTG TCCGTTCCTG 
CGGGAGAAGA GGGAGAACAA CGTGACGACG GTCCTACAGC CCTGCGAGGT TTTCTTCCAG 
AGCCGACAGT CAAACACTGA GCCGCAGTGC ATCGAGCTGT CCATCAGCGC ACTCACGCTC 
AAGGTGTCTC CGATCATCAT AAACACGGTC ATCACCATTC AGACGGCTCT AAGTCCTGCC 
GCTGAGACGC CGCAGGAGCT GGAGAGCCCC ATCCCACAGG ACCTATGGGA GCGCCGCAGC 
CTTCGCGACC TGAAGCTCTG GTTCCTGGAG GAGGAGGGCG AGGAGGTCAG CACGTCCGTC 
ACGCGTCTCA TTCCTCAGGG AGAGTCTCTG AAGATGAGCA TCGCCTCCAT CTGCCTGACG 
CTGGAGGCCG GAGTGGGACA CCGCACCGTC CCCATGCTGC TGGCCAAAGC CTCGTTCCAG 
GGGGACGTCA AGGACTGGAG CACTCTCATC AACCTGCACT GCCACCTCAG CATGGAGGTG 
AACTACTATA ATGAGATGAT GGGTTTATGG GAGCCGCTGT TGGAGCCGCT GGACGATGAG 
GATAAAGACG GTTTTAGACC CTGGACTCTC GCACTGAAGA TGAAGAAGAA GCCGGTGGTC 
AGTTCTGGGG TGTCTGATGA GGTGGACGGC GCTGTGCCTG ATTATAAAAC GGTCATCAGC 
TTCACTAGTA AAGACCAGCT TAACGTCACC CTGTCCAAGT GTGGTCTGCA CATGCTCAGT 
AATCTGGGAA CGGCTTTTGC AGAGGCCGCT AAACAGACGG CCGAGTGCTT TCAGACAGAT 
CAGACACCAT TCGTGGTGAA GAATCGCCTG GGTTTGGCGG TGTCTGTGCT GCACAGCGAA 
GTGTTTCGAC CCATCGGGAA GGAGTCTAAG GATCGTGTGG TGGAGCTCCC GGATGGAGAG 
AGTCTGAATT TGGACTATAC CCTCACCGCC GAGTCCAGCG ACCAGTTCTC TGCCATGACC 
AGCCTCAGTG CCAAAAACTA CATACAGCCC ACTCCAGTCG GCCACACGAC CGCAAGTGTG 
ATCCCGCTCA TCAAAACGGG CCGGGGCATG TACAGCGTCA TGCACAACGA GTCCCGCGTC 
ACTCGCTTCT TCGTCTGTCA GATTTACACC GTCGAGGGCA GCAAATATGT CAAGATTCGC 
TCTCCCTTCC AGATTATCAA CCACTTTTCA GTGCCGTTCC GTGTTTATGA AGGATCCGTG 
TTTCTGGGCG AGTCTCTGCC GACAGAGGAG TTCTGTGTGC CGCTGGGATC CTACAGAGCT 
GAGCTGAGTC TGCAGCCTGT GCCTGATGGC GGTGAAGAGT ACGAGCACTC CGAGAGCTTC 
ACCTACACTG AGGTTGTCCA GAAACCCGGA AAGTGTCTGG AGAAAAACTG CCATCTGCGT 
GGTGACAGCG CCCAGATTCT GAAGGTCAAC ATGGTTCCTC TTGCGGACAC AGTGACGTCC 
CACAGCAGCG AGGAGGAGTT CGATAAGCCC ATCGTACTGC ATTTGTGGCC CACCGTCCTG 
CTGCGCAACC TGCTGCCCTA CCCAATTACT TTCAAGCTAA AGGGCATCGG AGATTCCCCC 
TCGGTGTCGC TGAAGGAGGG CCACTCATCC CAGATCCACA CCGCCATCAT GGACTTCTCC 
GTGTTGGATC TGAGGCTGCT GGGATACTTG CAGCAGGACT GGGAGTCTCA GTACAGCCTC 
AGCAGTGAAC AAGACGAGAT CAGCTTCATT AAGTTTCGCT CGCTCGCACG TGAGGAGGAG 
ACTGAGAGCC GGCCGGAGCT GGACATCGCT ATGCATGTGA AGAAGGAGGA GGGTCAGGTT 
GTGCTGGCCG TACACTCGCC GTACTGGATG GTGAATAAAA CAAGCCGACT GCTGCAGTAC 
AAGGCGGATG ATATTCATCG CAAACACCCC AAGGATTACG ACATGCCGCT GCTCTTCTCC 
TTCAAGCCAC GCAACTTCCT GCAAAACAAC AAGATCCGCC TGATGATCTC AGAAAGTGAG 
CTGTCAGATG ATTTCTCCAT CGACACTGTC GGGAGTTACG GAGATGTCAA ATGCAAAGGC 
AGACAGAAGG ATTACTTGGT GGGAGTGAAG ATCGATGCTA GCAGCTTCAG CTTGACACGT 
TTGGTGACTT TTCTGCCATT CTACATGCTG GTGAACAGAA CCAAACATGT GATTCGGGTC 
TGTGAGGAGG GGCAGGAGAA CTGGACAGAA GCCCGACCCG GACAGGCTGC TGTTCCATTC 
TGGCCAGAAA GAGACTCAAA GAAGCTGAGA GTGAAGGTGG AGGGCAGTCA GTCGTCACCA 
CGCGCCTTTG ACTTCCACCA GCCAGAGAAC TGCCTCCTGC TGCATCTGGA CAAAAGCTTG 
GGTGGCATCA TTGTGGATGT GAATCTGACA GAGCATTCAG CTGTGATCCT CTTCTCTGAT 
TATCATGATG GAGCCGCTCC CTTCCTCCTC ATCAACCACA CTAAGGACGA GACGCTGCAG 
TACCACCAGA GCTCTCAGGC CGAGATCGAT GAACTGAGTC CAGGTCAGGC GGTTTACTAC 
ACCTGGGCTG AACCAACCGG CTCCAGGATC CTCAGCTGGA AACTGGGCAA ATACAAAGGA 
GAGCTCAAGA GTGAAGAGGA TCAGTTGCTG GATGTGGACA AGGAGGGCAA GTTGTTTTTG 
GTGTCGTTTT ATGAAGGCCT CCAGCGTGTG GCGCTCTTCA CCAGCGAGCA GCGCATCTTC 
AAGAAGATCT GCGACAGTGA GAAGATAGAG CTGGCTCAGC AAGAAATCAG CATCTTGCTG 
CAGAACATGG GCATTTCGCT GGTCAACAAC AACTCCAGCC AGGAAGTGGC CTTCATTGGC 
ATCACCAGCT CTGATGTTGT GTGGGAAACC AAGCCGAAGA AGCGCAGCCG CTGGAAAGTT 
CTGAGTGTTA AAGAGGCAGC AGTTCTGGAG CAGCACTACA GAGACTACAT GGAGAACGGC 
CCAGTGGACA GCACCATCAT AGACCTGGAC AACGACTACC AGGTGTTGTT TGCTGCTAAC 
GGTGTGGACA TGAGGATCAT GCAGCCCTAC GATGCTCCAC TCAGAAGGAA CTTCCTGCCG 
GCGTTGAAGG TGGAGTACAG CGTTTCAGCC CGCCAGAAAA CATACAGAGT CCAGATCAAC 
AGGATCCAGA TCCAAAACCA GCTGCAAGGA GCCATATTCC CGTTTGTGTT TTACCCCATC 
AAACCGCCAA AGTCTGTCAG CATGGACTCA GAGCCAAAAC CGCTGGCTGA CGTGAGCATC 
ATCACCAGAG CAGCAGGACA CTCCGACATT CTGCGCATCA AATATTTTAT GGTGTTGATT 
CAGGAGATGG ACCTGCGGCT GGATCTGGGC TTTCTGTATG CCATACTTGA GCTCTTCACT 
GCTGAACACA CCAGTGCTGT GACGTCTGAA CAGGAGGTGG AGTTGTTTGA TAAAGACATA 
GAGTATCTAA AGACTGAGTT AAATCATGTT TCAGCTACAG ACACATCTCC CATCAGCCTC 
TACGAGTACT TCCACATCTC TCCTATAAAG CTTCACTTGA GCTTGTCTCT GAGCAGTGGT 
GGTGAAGACA GCAATAAGCA AGAGCGCGAG ACGGAGCTGA TCCCAGTTCA GTCTCTCCAT 
CTGCTGCTCA AGAGCATCGG GGCTACACTT ACAGACGCAC AGGACGTCCT CTTTAAACTG 
GGCTTCTTCG AGCTGAGGTA TCAGTTTCGC ACCACTCAGC AGCTGCAGTG GGAGGTCGTT 
CGCCATTACT CCAAACAGGC TATCAAGCAG ATGTATGTCC TTGTTCTGGG TTTGGACGTT 
CTTGGGAATC CATTCGGGCT GATCCGGGGC CTGTCTGAAG GCGTCGAGGC TTTCTTTTAC 
GAGCCCTATC AGGGTGCAAT TCAGGGTCCA GAGGAGTTCA TGGAGGGCAT GGCTCTCGGC 
GTGAAGGCTC TGGTTGGAGG CGCTGTGGGT GGTCTGGCCG GAGCGGCGTC TCGCATCACT 
GGAGCGATGG CTAAAGGTGT GGCGGCGATG ACGATGGATG AAGAGTACCA GCAGAAGAGA 
CGAGAGGCCA TGAACAAACA GCCCAGCGGA CTGAGAGAAG GACTGACACG AGGAGGAAAA 
GGACTGGTGT CTGGCTTCGT GAGTGGCATC ACGGGCATTG TGACCAAACC CATTAAAGGA 
GCTCAGAAGG AAGGAGCGGC AGGTTTCTTC AAGGGTATGG GTAAGGGTTT AGTTGGAGCT 
CTGACCAGAC CAACAGGGGG CATCATTGAC ATGGCCAGCA GCACCTTCCA GGGCATCAAG 
AGAGCTGCAG AGCTGTCTCA GGATGTGGAG AGTCTGCGCC CTCCACGCTT CATCCACGAG 
GACGGAGTGA TCCGGCCGTA CAGAGAGAGA GAAGGCCTCG GCAGTCAGAT GCTGCAGAGT 
ATTGAAAACG GACGGTTTGC CAAGTACAGG TACTTTGCTC ACGCTAAAGT CAACGAGTCT 
GATTTCCTGA TGATCACTAA AAGGGGGATC TTTTTCGTGA CAAAGGGCAC GTTTGGTCAG 
TTGACATGTG AATGGCAGTA TCTGTTCGAT GAGTTTACTA AAGCACCCAT GATTGTGGAA 
GGCAGGAGAC TGCGCATCGA AGCGAAGGAG AGAGTCAAGT CAGTGTTTCA CGCCAAAGAG 
TTCGGGAAGA TCATCAATTT CAAAACTCCA GAATTAGCAA AGTGGGTTCT TGCCAAGCTT 
GAAGACGCTC GGGAGAATCT GCCCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302584.1
CDS	152..9781
Translation	MVFESVVVDVLNRFLGDYVGNLDSSQLSLGIWGGDAILRNLEIKENALSQLDIPFKVRAGHIGRLELKIPWKNLYTQSVEATLDEVYLLIVPTASIKYDAEKEERQLQESRQRELQRIEETKQKAAEKENPKGEKQDTFVEKLVTQVIKNLQVKISNIHVRYEDDVTNPSVPLSFGISLQNLSLQTADQNWNPCLLDEKAKLFFKLVRLDNLFAYWNVSSELYSNHNPEEALRYLKHSVAIQTAIPQDYHFIFRPISANAKLRMNPRSDVDFSSPKMDLVVNLPEVAVELSRPQYVSILELLGSVDMMTRNLPYRKYRPYVVVHTNARQWWRYVITGVLEVNVKPRLHMWSWLHIRRHRQHVKKYRELYKNKIISKKPAESLLKELEETERTLDIFNITMARQQAEMEASKAGLRIYRPGVKVEEEQSQGWFGWMWNWSGEASATQTKDVKTGGFDGLMTPAEKAKLYAAIGYSETAVNPNLPKNFENMKMNFHLLRLSLSIKESREKAEIIKLSVIDLKAMLTQRPAAQAIKISAQLSSFEVSGLPREKSAPILLSPRHVDGMKDKSLLNLMFETNPLDARADQRLRIESQPLEIIYDAMTVNSMSAFFMPPKDLQLDELTNATLMKLEQFRDKTSTGLMYIIETQKVLDLKINLMASYVIVPENGFYDPKHNLAILDLGHFQMSSQSRADLPQLSAGSSTLEDIMSRAYDSFDVQLSSLQFLYSKAGGDWKRARPLRQSAFHILEPVDLKVVFSRAMVVTDSRMPKFKMHGELPLLSLRISDDKVRGVLALLSSIPLPESRPTPQSRNTQATPKASKRGNTPQLTPRRPAIDDLRASIIYGSEEELFFDAPSSPIEDLPFFEASSDSRHASAADKRLSMVLKDPKKNMTEFLLSFEINKLSVQLCRVSNRQEVSVLHLFIDGLGTELKLRTFDMSSYTFLREICLECSEYLDSEDKKVHLITTLDNTEEDLLTLEYVKADKNAPDFKAMYKNTEQLIKVNFSSLDIHLHTEALLNSMNFLNNLLPPMKKETQEEQPIIPKEEEEEEEEEEEEEEAKVENSAVAKKSSYKKSKFEDVVNLHIRADLNCLKVFIRGEKARISEISIEGLVSEVQMKKKSVEILANLKNIIILDCDKEALYKKAVSIADKEVFDFRMVNHVDATEGDAYTDMHIVDTSVTLTVGCIQVIFLNKFMSSILAFINNFQEAKNALAEVTAQAAEKAATGVLELAERSTRISLDVDIKAPVVFLPQSSVSHNVIVADLGLVTVKNRFEIVSSPTHTKIPPVMDIMAVGLSDLKMYRTTFTDDKFNSEIQLLKPVNMDLSIKRNLSASWYHSIPDIQINAHLKPMSLILSQEDLTVVLKTLSENLSETSDAPPAPSSSDKGDTVSNKGSQGSGGNTVVTAAVVEGQKSGKLKNTLKLNFKFDSMTLVLYSPNSEIQLSDSRDESLKLAEFTLGTISTSVHMFSDSSMKASLKLTNCLLDDKRQGVKMITSRMMAMRQDREDSVMVDVSYRQSRDGTRLDTLVQDLYLCASMEFLLTVADVFLKALSDGFSGPPKSNKQPASTSKDPAAPAPPVVAKTEMNVVVKNPEIVFVADLTRAEAPALVMTTQCELVMKSEPSGQCMTAAISDLKIVACPFLREKRENNVTTVLQPCEVFFQSRQSNTEPQCIELSISALTLKVSPIIINTVITIQTALSPAAETPQELESPIPQDLWERRSLRDLKLWFLEEEGEEVSTSVTRLIPQGESLKMSIASICLTLEAGVGHRTVPMLLAKASFQGDVKDWSTLINLHCHLSMEVNYYNEMMGLWEPLLEPLDDEDKDGFRPWTLALKMKKKPVVSSGVSDEVDGAVPDYKTVISFTSKDQLNVTLSKCGLHMLSNLGTAFAEAAKQTAECFQTDQTPFVVKNRLGLAVSVLHSEVFRPIGKESKDRVVELPDGESLNLDYTLTAESSDQFSAMTSLSAKNYIQPTPVGHTTASVIPLIKTGRGMYSVMHNESRVTRFFVCQIYTVEGSKYVKIRSPFQIINHFSVPFRVYEGSVFLGESLPTEEFCVPLGSYRAELSLQPVPDGGEEYEHSESFTYTEVVQKPGKCLEKNCHLRGDSAQILKVNMVPLADTVTSHSSEEEFDKPIVLHLWPTVLLRNLLPYPITFKLKGIGDSPSVSLKEGHSSQIHTAIMDFSVLDLRLLGYLQQDWESQYSLSSEQDEISFIKFRSLAREEETESRPELDIAMHVKKEEGQVVLAVHSPYWMVNKTSRLLQYKADDIHRKHPKDYDMPLLFSFKPRNFLQNNKIRLMISESELSDDFSIDTVGSYGDVKCKGRQKDYLVGVKIDASSFSLTRLVTFLPFYMLVNRTKHVIRVCEEGQENWTEARPGQAAVPFWPERDSKKLRVKVEGSQSSPRAFDFHQPENCLLLHLDKSLGGIIVDVNLTEHSAVILFSDYHDGAAPFLLINHTKDETLQYHQSSQAEIDELSPGQAVYYTWAEPTGSRILSWKLGKYKGELKSEEDQLLDVDKEGKLFLVSFYEGLQRVALFTSEQRIFKKICDSEKIELAQQEISILLQNMGISLVNNNSSQEVAFIGITSSDVVWETKPKKRSRWKVLSVKEAAVLEQHYRDYMENGPVDSTIIDLDNDYQVLFAANGVDMRIMQPYDAPLRRNFLPALKVEYSVSARQKTYRVQINRIQIQNQLQGAIFPFVFYPIKPPKSVSMDSEPKPLADVSIITRAAGHSDILRIKYFMVLIQEMDLRLDLGFLYAILELFTAEHTSAVTSEQEVELFDKDIEYLKTELNHVSATDTSPISLYEYFHISPIKLHLSLSLSSGGEDSNKQERETELIPVQSLHLLLKSIGATLTDAQDVLFKLGFFELRYQFRTTQQLQWEVVRHYSKQAIKQMYVLVLGLDVLGNPFGLIRGLSEGVEAFFYEPYQGAIQGPEEFMEGMALGVKALVGGAVGGLAGAASRITGAMAKGVAAMTMDEEYQQKRREAMNKQPSGLREGLTRGGKGLVSGFVSGITGIVTKPIKGAQKEGAAGFFKGMGKGLVGALTRPTGGIIDMASSTFQGIKRAAELSQDVESLRPPRFIHEDGVIRPYREREGLGSQMLQSIENGRFAKYRYFAHAKVNESDFLMITKRGIFFVTKGTFGQLTCEWQYLFDEFTKAPMIVEGRRLRIEAKERVKSVFHAKEFGKIINFKTPELAKWVLAKLEDARENLPK

Target ORF information:

RefSeq Version	XM_009304309.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio vacuolar protein sorting 13 homolog A (S. cerevisiae) (vps13a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304309.2

ORF Insert Sequence:

ATGGTGTTTG AGTCGGTGGT GGTCGACGTG CTGAACCGGT TTCTGGGGGA CTATGTTGGA 
AACCTCGACA GCTCGCAGCT CAGCCTCGGC ATATGGGGAG GTGATGCCAT ATTGAGGAAC 
CTCGAGATTA AAGAAAATGC ATTGAGCCAG CTGGACATTC CTTTCAAAGT CAGAGCAGGA 
CACATTGGGC GTTTGGAGTT AAAGATCCCC TGGAAGAACC TGTACACTCA GTCTGTGGAG 
GCCACGCTGG ATGAAGTTTA CCTGCTCATC GTACCTACTG CTAGTATAAA GTATGACGCA 
GAGAAGGAGG AGCGGCAGCT GCAGGAGTCT CGGCAGAGGG AGCTCCAGCG CATCGAGGAG 
ACCAAACAGA AAGCAGCAGA AAAAGAGAAT CCAAAGGGTG AGAAGCAGGA CACATTTGTG 
GAGAAACTGG TAACACAGGT GATCAAAAAC CTGCAGGTCA AAATATCCAA CATTCACGTC 
CGCTATGAGG ATGATGTCAC CAATCCCAGT GTTCCTCTCT CCTTTGGGAT ATCATTACAA 
AACCTCAGTC TCCAGACGGC GGATCAGAAC TGGAACCCCT GTCTTCTGGA TGAGAAAGCC 
AAACTGTTTT TTAAGCTTGT ACGTCTGGAT AATCTGTTTG CGTACTGGAA CGTCAGTTCA 
GAGCTGTACT CCAACCACAA CCCTGAGGAG GCTCTGCGAT ACCTGAAGCA CAGCGTAGCC 
ATCCAGACTG CCATCCCTCA GGATTATCAC TTCATCTTTC GTCCAATATC TGCCAATGCC 
AAGTTGCGGA TGAACCCTCG CTCTGATGTA GACTTCTCCT CACCCAAAAT GGACCTGGTG 
GTCAACCTTC CTGAAGTGGC CGTGGAGCTC AGCCGACCAC AGTACGTCAG CATTCTGGAG 
CTGTTGGGGT CGGTGGATAT GATGACCCGT AACCTTCCTT ACAGGAAGTA TCGGCCATAT 
GTGGTGGTTC ACACTAACGC TCGTCAGTGG TGGCGATATG TGATCACGGG TGTTCTGGAG 
GTCAATGTGA AGCCTCGTCT TCACATGTGG TCTTGGCTGC ATATTCGTCG ACATCGGCAG 
CATGTGAAAA AGTACAGAGA GCTTTATAAG AATAAAATCA TCAGTAAGAA ACCGGCAGAG 
AGTCTGCTGA AGGAGCTGGA GGAGACTGAA AGAACATTAG ACATATTCAA CATCACAATG 
GCGAGACAGC AGGCTGAGAT GGAGGCCTCG AAGGCAGGGC TCAGGATTTA CCGGCCCGGG 
GTGAAGGTGG AGGAGGAACA GTCACAGGGC TGGTTTGGCT GGATGTGGAA CTGGTCTGGA 
GAAGCCAGTG CCACTCAAAC CAAAGACGTC AAAACTGGAG GTTTTGATGG GTTAATGACG 
CCTGCAGAGA AAGCCAAACT ATACGCTGCC ATCGGATACA GCGAAACTGC TGTCAACCCC 
AACCTGCCAA AAAATTTTGA AAACATGAAG ATGAATTTCC ATCTTCTCCG CCTGTCCCTG 
TCCATCAAAG AGAGCCGAGA GAAGGCGGAG ATCATCAAAC TGTCTGTCAT TGACCTGAAG 
GCCATGCTGA CTCAAAGACC CGCGGCTCAA GCTATAAAGA TCTCCGCTCA GCTGTCGTCG 
TTTGAAGTGT CCGGTCTCCC GCGGGAAAAG TCGGCCCCGA TCCTGCTTTC TCCACGTCAT 
GTGGACGGCA TGAAGGATAA AAGTCTGCTG AACCTCATGT TCGAGACCAA CCCGCTGGAC 
GCTCGTGCTG ACCAGAGACT GCGCATCGAG TCCCAGCCGC TGGAGATCAT CTATGATGCA 
ATGACCGTGA ACAGTATGTC CGCTTTCTTC ATGCCTCCTA AAGACCTGCA GCTGGATGAA 
CTGACCAACG CCACACTGAT GAAGCTGGAG CAGTTCAGAG ACAAAACCTC CACAGGCCTC 
ATGTACATCA TTGAGACGCA GAAAGTTCTG GATCTGAAAA TAAACCTCAT GGCCTCGTAT 
GTCATCGTAC CGGAAAACGG CTTTTATGAC CCCAAACACA ACCTTGCCAT TCTAGATCTG 
GGTCATTTTC AGATGTCAAG TCAGAGCCGA GCTGATCTTC CTCAGCTGTC TGCGGGCAGC 
AGTACTCTAG AGGACATCAT GTCTCGAGCG TACGACAGCT TCGACGTCCA GCTCAGCAGT 
CTGCAGTTCC TCTACTCCAA AGCAGGTGGA GACTGGAAGC GGGCTCGTCC GCTAAGACAG 
TCTGCTTTCC ACATCCTGGA GCCAGTGGAC CTGAAGGTGG TCTTTAGCAG AGCGATGGTG 
GTCACAGACT CCAGGATGCC CAAGTTTAAG ATGCACGGTG AGCTGCCTCT GCTGTCTCTG 
CGGATCTCTG ATGATAAAGT GCGTGGCGTT CTGGCTCTGC TCAGCAGTAT TCCTCTACCT 
GAGAGCCGGC CCACGCCTCA GTCCAGAAAC ACGCAGGCCA CTCCAAAGGC CTCCAAGCGA 
GGAAATACCC CTCAGCTCAC GCCCCGAAGG CCAGCTATAG ATGACCTGCG TGCGTCCATC 
ATATACGGCT CAGAGGAGGA GCTGTTCTTT GACGCCCCCA GTTCACCGAT CGAGGATCTG 
CCGTTCTTCG AGGCCTCGTC GGACTCTCGG CATGCTTCAG CAGCTGATAA ACGTCTGTCT 
ATGGTGCTCA AAGACCCCAA GAAGAACATG ACTGAGTTTC TGCTTAGTTT TGAGATCAAC 
AAGCTCTCGG TGCAGCTGTG CCGTGTGTCG AATAGACAGG AAGTGAGTGT TCTGCATCTG 
TTCATCGATG GATTGGGAAC AGAGCTCAAA CTTCGCACGT TTGACATGTC GTCGTACACT 
TTCCTGCGTG AGATCTGCCT CGAGTGCTCA GAATACTTGG ATTCTGAGGA CAAGAAAGTT 
CACCTTATCA CCACGCTGGA CAACACAGAG GAGGATCTGC TCACTCTGGA GTATGTCAAG 
GCAGACAAAA ATGCACCAGA TTTTAAAGCT ATGTACAAAA ATACAGAGCA ACTAATCAAG 
GTGAATTTCT CCTCTCTGGA CATTCACCTG CACACAGAGG CTCTCCTGAA CTCCATGAAC 
TTCCTAAATA ATCTTCTGCC ACCGATGAAG AAGGAGACCC AGGAGGAACA ACCAATAATT 
CCCAAGGAGG AAGAGGAGGA GGAGGAGGAA GAAGAAGAAG AGGAGGAGGC TAAAGTAGAG 
AATTCTGCTG TGGCCAAGAA GTCTTCCTAC AAGAAGTCAA AGTTTGAGGA TGTGGTGAAT 
TTGCACATCC GCGCAGACCT CAACTGTCTG AAGGTGTTCA TACGAGGAGA GAAAGCCAGG 
ATCTCTGAGA TCAGCATAGA GGGACTGGTT TCTGAAGTTC AGATGAAGAA GAAGTCTGTG 
GAGATTCTGG CTAACTTGAA GAACATCATC ATTCTGGATT GTGACAAAGA GGCACTTTAT 
AAAAAGGCAG TGTCCATCGC TGATAAGGAG GTGTTTGATT TCCGCATGGT GAACCATGTC 
GATGCAACGG AGGGTGACGC TTACACAGAC ATGCACATTG TGGACACTAG CGTAACCCTG 
ACCGTGGGCT GCATTCAAGT TATTTTCCTC AACAAGTTTA TGTCCTCAAT ACTGGCATTT 
ATTAATAATT TCCAGGAGGC TAAAAATGCA TTGGCGGAGG TGACGGCCCA GGCAGCAGAG 
AAAGCAGCCA CGGGGGTTCT GGAGCTGGCA GAGAGAAGTA CTCGGATCTC CCTGGACGTC 
GACATCAAAG CTCCTGTTGT CTTCCTCCCA CAATCCTCAG TTTCCCACAA CGTCATTGTG 
GCAGATCTGG GTTTAGTGAC TGTAAAGAAC CGCTTTGAGA TCGTCTCCTC TCCAACACAC 
ACCAAGATCC CTCCTGTCAT GGACATCATG GCTGTCGGAT TGAGTGATCT GAAAATGTAC 
AGGACAACGT TCACTGATGA CAAGTTTAAC AGCGAGATTC AGTTGCTCAA GCCAGTCAAC 
ATGGACCTGT CAATCAAACG CAATCTCTCC GCCTCCTGGT ATCACAGCAT ACCCGACATC 
CAGATCAATG CTCACCTCAA GCCCATGAGC CTGATCTTGA GTCAGGAGGA TCTGACTGTG 
GTGCTGAAGA CTCTGAGTGA GAATCTGTCC GAGACGTCTG ATGCTCCTCC AGCTCCGTCC 
AGCTCTGATA AAGGAGATAC GGTTTCTAAC AAAGGCTCCC AGGGTTCTGG AGGCAACACC 
GTTGTGACCG CAGCAGTGGT GGAGGGTCAG AAATCAGGCA AACTGAAGAA CACCCTAAAA 
CTGAACTTCA AGTTTGACTC CATGACGCTG GTCCTCTACA GCCCGAACAG CGAGATCCAG 
TTGTCCGACT CTCGTGATGA GAGTTTGAAG TTGGCCGAGT TCACACTGGG CACCATCTCC 
ACCAGTGTGC ACATGTTCTC CGACAGCTCC ATGAAGGCCT CGCTCAAACT CACCAACTGT 
CTGCTGGATG ACAAGAGGCA GGGAGTGAAG ATGATTACAT CCAGGATGAT GGCGATGCGT 
CAGGACCGAG AGGACAGTGT GATGGTGGAC GTCAGCTACC GCCAGAGTCG GGACGGGACT 
CGTCTGGACA CCCTCGTGCA AGACCTTTAT CTTTGTGCCA GTATGGAGTT TCTGCTCACT 
GTAGCTGATG TGTTTCTTAA AGCTCTGTCT GACGGCTTCT CCGGTCCTCC AAAGAGTAAC 
AAACAACCGG CCAGCACCTC TAAAGACCCA GCGGCTCCTG CTCCACCAGT GGTTGCTAAA 
ACCGAGATGA ATGTGGTGGT GAAGAACCCA GAGATCGTGT TTGTGGCGGA TCTGACGCGT 
GCTGAAGCTC CGGCTCTGGT CATGACCACT CAGTGTGAGC TGGTGATGAA GAGCGAGCCC 
AGCGGTCAGT GCATGACGGC CGCCATTTCA GACCTGAAGA TTGTGGCCTG TCCGTTCCTG 
CGGGAGAAGA GGGAGAACAA CGTGACGACG GTCCTACAGC CCTGCGAGGT TTTCTTCCAG 
AGCCGACAGT CAAACACTGA GCCGCAGTGC ATCGAGCTGT CCATCAGCGC ACTCACGCTC 
AAGGTGTCTC CGATCATCAT AAACACGGTC ATCACCATTC AGACGGCTCT AAGTCCTGCC 
GCTGAGACGC CGCAGGAGCT GGAGAGCCCC ATCCCACAGG ACCTATGGGA GCGCCGCAGC 
CTTCGCGACC TGAAGCTCTG GTTCCTGGAG GAGGAGGGCG AGGAGGTCAG CACGTCCGTC 
ACGCGTCTCA TTCCTCAGGG AGAGTCTCTG AAGATGAGCA TCGCCTCCAT CTGCCTGACG 
CTGGAGGCCG GAGTGGGACA CCGCACCGTC CCCATGCTGC TGGCCAAAGC CTCGTTCCAG 
GGGGACGTCA AGGACTGGAG CACTCTCATC AACCTGCACT GCCACCTCAG CATGGAGGTG 
AACTACTATA ATGAGATGAT GGGTTTATGG GAGCCGCTGT TGGAGCCGCT GGACGATGAG 
GATAAAGACG GTTTTAGACC CTGGACTCTC GCACTGAAGA TGAAGAAGAA GCCGGTGGTC 
AGTTCTGGGG TGTCTGATGA GGTGGACGGC GCTGTGCCTG ATTATAAAAC GGTCATCAGC 
TTCACTAGTA AAGACCAGCT TAACGTCACC CTGTCCAAGT GTGGTCTGCA CATGCTCAGT 
AATCTGGGAA CGGCTTTTGC AGAGGCCGCT AAACAGACGG CCGAGTGCTT TCAGACAGAT 
CAGACACCAT TCGTGGTGAA GAATCGCCTG GGTTTGGCGG TGTCTGTGCT GCACAGCGAA 
GTGTTTCGAC CCATCGGGAA GGAGTCTAAG GATCGTGTGG TGGAGCTCCC GGATGGAGAG 
AGTCTGAATT TGGACTATAC CCTCACCGCC GAGTCCAGCG ACCAGTTCTC TGCCATGACC 
AGCCTCAGTG CCAAAAACTA CATACAGCCC ACTCCAGTCG GCCACACGAC CGCAAGTGTG 
ATCCCGCTCA TCAAAACGGG CCGGGGCATG TACAGCGTCA TGCACAACGA GTCCCGCGTC 
ACTCGCTTCT TCGTCTGTCA GATTTACACC GTCGAGGGCA GCAAATATGT CAAGATTCGC 
TCTCCCTTCC AGATTATCAA CCACTTTTCA GTGCCGTTCC GTGTTTATGA AGGATCCGTG 
TTTCTGGGCG AGTCTCTGCC GACAGAGGAG TTCTGTGTGC CGCTGGGATC CTACAGAGCT 
GAGCTGAGTC TGCAGCCTGT GCCTGATGGC GGTGAAGAGT ACGAGCACTC CGAGAGCTTC 
ACCTACACTG AGGTTGTCCA GAAACCCGGA AAGTGTCTGG AGAAAAACTG CCATCTGCGT 
GGTGACAGCG CCCAGATTCT GAAGGTCAAC ATGGTTCCTC TTGCGGACAC AGTGACGTCC 
CACAGCAGCG AGGAGGAGTT CGATAAGCCC ATCGTACTGC ATTTGTGGCC CACCGTCCTG 
CTGCGCAACC TGCTGCCCTA CCCAATTACT TTCAAGCTAA AGGGCATCGG AGATTCCCCC 
TCGGTGTCGC TGAAGGAGGG CCACTCATCC CAGATCCACA CCGCCATCAT GGACTTCTCC 
GTGTTGGATC TGAGGCTGCT GGGATACTTG CAGCAGGACT GGGAGTCTCA GTACAGCCTC 
AGCAGTGAAC AAGACGAGAT CAGCTTCATT AAGTTTCGCT CGCTCGCACG TGAGGAGGAG 
ACTGAGAGCC GGCCGGAGCT GGACATCGCT ATGCATGTGA AGAAGGAGGA GGGTCAGGTT 
GTGCTGGCCG TACACTCGCC GTACTGGATG GTGAATAAAA CAAGCCGACT GCTGCAGTAC 
AAGGCGGATG ATATTCATCG CAAACACCCC AAGGATTACG ACATGCCGCT GCTCTTCTCC 
TTCAAGCCAC GCAACTTCCT GCAAAACAAC AAGATCCGCC TGATGATCTC AGAAAGTGAG 
CTGTCAGATG ATTTCTCCAT CGACACTGTC GGGAGTTACG GAGATGTCAA ATGCAAAGGC 
AGACAGAAGG ATTACTTGGT GGGAGTGAAG ATCGATGCTA GCAGCTTCAG CTTGACACGT 
TTGGTGACTT TTCTGCCATT CTACATGCTG GTGAACAGAA CCAAACATGT GATTCGGGTC 
TGTGAGGAGG GGCAGGAGAA CTGGACAGAA GCCCGACCCG GACAGGCTGC TGTTCCATTC 
TGGCCAGAAA GAGACTCAAA GAAGCTGAGA GTGAAGGTGG AGGGCAGTCA GTCGTCACCA 
CGCGCCTTTG ACTTCCACCA GCCAGAGAAC TGCCTCCTGC TGCATCTGGA CAAAAGCTTG 
GGTGGCATCA TTGTGGATGT GAATCTGACA GAGCATTCAG CTGTGATCCT CTTCTCTGAT 
TATCATGATG GAGCCGCTCC CTTCCTCCTC ATCAACCACA CTAAGGACGA GACGCTGCAG 
TACCACCAGA GCTCTCAGGC CGAGATCGAT GAACTGAGTC CAGGTCAGGC GGTTTACTAC 
ACCTGGGCTG AACCAACCGG CTCCAGGATC CTCAGCTGGA AACTGGGCAA ATACAAAGGA 
GAGCTCAAGA GTGAAGAGGA TCAGTTGCTG GATGTGGACA AGGAGGGCAA GTTGTTTTTG 
GTGTCGTTTT ATGAAGGCCT CCAGCGTGTG GCGCTCTTCA CCAGCGAGCA GCGCATCTTC 
AAGAAGATCT GCGACAGTGA GAAGATAGAG CTGGCTCAGC AAGAAATCAG CATCTTGCTG 
CAGAACATGG GCATTTCGCT GGTCAACAAC AACTCCAGCC AGGAAGTGGC CTTCATTGGC 
ATCACCAGCT CTGATGTTGT GTGGGAAACC AAGCCGAAGA AGCGCAGCCG CTGGAAAGTT 
CTGAGTGTTA AAGAGGCAGC AGTTCTGGAG CAGCACTACA GAGACTACAT GGAGAACGGC 
CCAGTGGACA GCACCATCAT AGACCTGGAC AACGACTACC AGGTGTTGTT TGCTGCTAAC 
GGTGTGGACA TGAGGATCAT GCAGCCCTAC GATGCTCCAC TCAGAAGGAA CTTCCTGCCG 
GCGTTGAAGG TGGAGTACAG CGTTTCAGCC CGCCAGAAAA CATACAGAGT CCAGATCAAC 
AGGATCCAGA TCCAAAACCA GCTGCAAGGA GCCATATTCC CGTTTGTGTT TTACCCCATC 
AAACCGCCAA AGTCTGTCAG CATGGACTCA GAGCCAAAAC CGCTGGCTGA CGTGAGCATC 
ATCACCAGAG CAGCAGGACA CTCCGACATT CTGCGCATCA AATATTTTAT GGTGTTGATT 
CAGGAGATGG ACCTGCGGCT GGATCTGGGC TTTCTGTATG CCATACTTGA GCTCTTCACT 
GCTGAACACA CCAGTGCTGT GACGTCTGAA CAGGAGGTGG AGTTGTTTGA TAAAGACATA 
GAGTATCTAA AGACTGAGTT AAATCATGTT TCAGCTACAG ACACATCTCC CATCAGCCTC 
TACGAGTACT TCCACATCTC TCCTATAAAG CTTCACTTGA GCTTGTCTCT GAGCAGTGGT 
GGTGAAGACA GCAATAAGCA AGAGCGCGAG ACGGAGCTGA TCCCAGTTCA GTCTCTCCAT 
CTGCTGCTCA AGAGCATCGG GGCTACACTT ACAGACGCAC AGGACGTCCT CTTTAAACTG 
GGCTTCTTCG AGCTGAGGTA TCAGTTTCGC ACCACTCAGC AGCTGCAGTG GGAGGTCGTT 
CGCCATTACT CCAAACAGGC TATCAAGCAG ATGTATGTCC TTGTTCTGGG TTTGGACGTT 
CTTGGGAATC CATTCGGGCT GATCCGGGGC CTGTCTGAAG GCGTCGAGGC TTTCTTTTAC 
GAGCCCTATC AGGGTGCAAT TCAGGGTCCA GAGGAGTTCA TGGAGGGCAT GGCTCTCGGC 
GTGAAGGCTC TGGTTGGAGG CGCTGTGGGT GGTCTGGCCG GAGCGGCGTC TCGCATCACT 
GGAGCGATGG CTAAAGGTGT GGCGGCGATG ACGATGGATG AAGAGTACCA GCAGAAGAGA 
CGAGAGGCCA TGAACAAACA GCCCAGCGGA CTGAGAGAAG GACTGACACG AGGAGGAAAA 
GGACTGGTGT CTGGCTTCGT GAGTGGCATC ACGGGCATTG TGACCAAACC CATTAAAGGA 
GCTCAGAAGG AAGGAGCGGC AGGTTTCTTC AAGGGTATGG GTAAGGGTTT AGTTGGAGCT 
CTGACCAGAC CAACAGGGGG CATCATTGAC ATGGCCAGCA GCACCTTCCA GGGCATCAAG 
AGAGCTGCAG AGCTGTCTCA GGATGTGGAG AGTCTGCGCC CTCCACGCTT CATCCACGAG 
GACGGAGTGA TCCGGCCGTA CAGAGAGAGA GAAGGCCTCG GCAGTCAGAT GCTGCAGAGT 
ATTGAAAACG GACGGTTTGC CAAGTACAGG TACTTTGCTC ACGCTAAAGT CAACGAGTCT 
GATTTCCTGA TGATCACTAA AAGGGGGATC TTTTTCGTGA CAAAGGGCAC GTTTGGTCAG 
TTGACATGTG AATGGCAGTA TCTGTTCGAT GAGTTTACTA AAGCACCCAT GATTGTGGAA 
GGCAGGAGAC TGCGCATCGA AGCGAAGGAG AGAGTCAAGT CAGTGTTTCA CGCCAAAGAG 
TTCGGGAAGA TCATCAATTT CAAAACTCCA GAATTAGCAA AGTGGGTTCT TGCCAAGCTT 
GAAGACGCTC GGGAGAATCT GCCCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS13A ( XM_009304310.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009302585.1 Danio rerio vacuolar protein sorting 13 homolog A (S. cerevisiae) (vps13a), transcript variant X3, mRNA.

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CloneID	ODa40322
Clone ID Related Accession (Same CDS sequence)	XM_009304310.2 , XM_009304310.3
Accession Version	XM_009304310.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	vacuolar protein sorting-associated protein 13A isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304310.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGTTTG AGTCGGTGGT GGTCGACGTG CTGAACCGGT TTCTGGGGGA CTATGTTGGA 
AACCTCGACA GCTCGCAGCT CAGCCTCGGC ATATGGGGAG GTGATGCCAT ATTGAGGAAC 
CTCGAGATTA AAGAAAATGC ATTGAGCCAG CTGGACATTC CTTTCAAAGT CAGAGCAGGA 
CACATTGGGC GTTTGGAGTT AAAGATCCCC TGGAAGAACC TGTACACTCA GTCTGTGGAG 
GCCACGCTGG ATGAAGTTTA CCTGCTCATC GTACCTACTG CTAGTATAAA GTATGACGCA 
GAGAAGGAGG AGCGGCAGCT GCAGGAGTCT CGGCAGAGGG AGCTCCAGCG CATCGAGGAG 
ACCAAACAGA AAGCAGCAGA AAAAGAGAAT CCAAAGGGTG AGAAGCAGGA CACATTTGTG 
GAGAAACTGG TAACACAGGT GATCAAAAAC CTGCAGGTCA AAATATCCAA CATTCACGTC 
CGCTATGAGG ATGATGTCAC CAATCCCAGT GTTCCTCTCT CCTTTGGGAT ATCATTACAA 
AACCTCAGTC TCCAGACGGC GGATCAGAAC TGGAACCCCT GTCTTCTGGA TGAGAAAGCC 
AAACTGTTTT TTAAGCTTGT ACGTCTGGAT AATCTGTTTG CGTACTGGAA CGTCAGTTCA 
GAGCTGTACT CCAACCACAA CCCTGAGGAG GCTCTGCGAT ACCTGAAGCA CAGCGTAGCC 
ATCCAGACTG CCATCCCTCA GGATTATCAC TTCATCTTTC GTCCAATATC TGCCAATGCC 
AAGTTGCGGA TGAACCCTCG CTCTGATGTA GACTTCTCCT CACCCAAAAT GGACCTGGTG 
GTCAACCTTC CTGAAGTGGC CGTGGAGCTC AGCCGACCAC AGTACGTCAG CATTCTGGAG 
CTGTTGGGGT CGGTGGATAT GATGACCCGT AACCTTCCTT ACAGGAAGTA TCGGCCATAT 
GTGGTGGTTC ACACTAACGC TCGTCAGTGG TGGCGATATG TGATCACGGG TGTTCTGGAG 
GTCAATGTGA AGCCTCGTCT TCACATGTGG TCTTGGCTGC ATATTCGTCG ACATCGGCAG 
CATGTGAAAA AGTACAGAGA GCTTTATAAG AATAAAATCA TCAGTAAGAA ACCGGCAGAG 
AGTCTGCTGA AGGAGCTGGA GGAGACTGAA AGAACATTAG ACATATTCAA CATCACAATG 
GCGAGACAGC AGGCTGAGAT GGAGGCCTCG AAGGCAGGGC TCAGGATTTA CCGGCCCGGG 
GTGAAGGTGG AGGAGGAACA GTCACAGGGC TGGTTTGGCT GGATGTGGAA CTGGTCTGGA 
GAAGCCAGTG CCACTCAAAC CAAAGACGTC AAAACTGGAG GTTTTGATGG GTTAATGACG 
CCTGCAGAGA AAGCCAAACT ATACGCTGCC ATCGGATACA GCGAAACTGC TGTCAACCCC 
AACCTGCCAA AAAATTTTGA AAACATGAAG ATGAATTTCC ATCTTCTCCG CCTGTCCCTG 
TCCATCAAAG AGAGCCGAGA GAAGGCGGAG ATCATCAAAC TGTCTGTCAT TGACCTGAAG 
GCCATGCTGA CTCAAAGACC CGCGGCTCAA GCTATAAAGA TCTCCGCTCA GCTGTCGTCG 
TTTGAAGTGT CCGGTCTCCC GCGGGAAAAG TCGGCCCCGA TCCTGCTTTC TCCACGTCAT 
GTGGACGGCA TGAAGGATAA AAGTCTGCTG AACCTCATGT TCGAGACCAA CCCGCTGGAC 
GCTCGTGCTG ACCAGAGACT GCGCATCGAG TCCCAGCCGC TGGAGATCAT CTATGATGCA 
ATGACCGTGA ACAGTATGTC CGCTTTCTTC ATGCCTCCTA AAGACCTGCA GCTGGATGAA 
CTGACCAACG CCACACTGAT GAAGCTGGAG CAGTTCAGAG ACAAAACCTC CACAGGCCTC 
ATGTACATCA TTGAGACGCA GAAAGTTCTG GATCTGAAAA TAAACCTCAT GGCCTCGTAT 
GTCATCGTAC CGGAAAACGG CTTTTATGAC CCCAAACACA ACCTTGCCAT TCTAGATCTG 
GGTCATTTTC AGATGTCAAG TCAGAGCCGA GCTGATCTTC CTCAGCTGTC TGCGGGCAGC 
AGTACTCTAG AGGACATCAT GTCTCGAGCG TACGACAGCT TCGACGTCCA GCTCAGCAGT 
CTGCAGTTCC TCTACTCCAA AGCAGGTGGA GACTGGAAGC GGGCTCGTCC GCTAAGACAG 
TCTGCTTTCC ACATCCTGGA GCCAGTGGAC CTGAAGGTGG TCTTTAGCAG AGCGATGGTG 
GTCACAGACT CCAGGATGCC CAAGTTTAAG ATGCACGGTG AGCTGCCTCT GCTGTCTCTG 
CGGATCTCTG ATGATAAAGT GCGTGGCGTT CTGGCTCTGC TCAGCAGTAT TCCTCTACCT 
GAGAGCCGGC CCACGCCTCA GTCCAGAAAC ACGCAGGCCA CTCCAAAGGC CTCCAAGCGA 
GGAAATACCC CTCAGCTCAC GCCCCGAAGG CCAGCTATAG ATGACCTGCG TGCGTCCATC 
ATATACGGCT CAGAGGAGGA GCTGTTCTTT GACGCCCCCA GTTCACCGAT CGAGGATCTG 
CCGTTCTTCG AGGCCTCGTC GGACTCTCGG CATGCTTCAG CAGCTGATAA ACGTCTGTCT 
ATGGTGCTCA AAGACCCCAA GAAGAACATG ACTGAGTTTC TGCTTAGTTT TGAGATCAAC 
AAGCTCTCGG TGCAGCTGTG CCGTGTGTCG AATAGACAGG AAGTGAGTGT TCTGCATCTG 
TTCATCGATG GATTGGGAAC AGAGCTCAAA CTTCGCACGT TTGACATGTC GTCGTACACT 
TTCCTGCGTG AGATCTGCCT CGAGTGCTCA GAATACTTGG ATTCTGAGGA CAAGAAAGTT 
CACCTTATCA CCACGCTGGA CAACACAGAG GAGGATCTGC TCACTCTGGA GTATGTCAAG 
GCAGACAAAA ATGCACCAGA TTTTAAAGCT ATGTACAAAA ATACAGAGCA ACTAATCAAG 
GTGAATTTCT CCTCTCTGGA CATTCACCTG CACACAGAGG CTCTCCTGAA CTCCATGAAC 
TTCCTAAATA ATCTTCTGCC ACCGATGAAG AAGGAGACCC AGGAGGAACA ACCAATAATT 
CCCAAGGAGG AAGAGGAGGA GGAGGAGGAA GAAGAAGAAG AGGAGGAGGC TAAAGTAGAG 
AATTCTGCTG TGGCCAAGAA GTCTTCCTAC AAGAAGTCAA AGTTTGAGGA TGTGGTGAAT 
TTGCACATCC GCGCAGACCT CAACTGTCTG AAGGTGTTCA TACGAGGAGA GAAAGCCAGG 
ATCTCTGAGA TCAGCATAGA GGGACTGGTT TCTGAAGTTC AGATGAAGAA GAAGTCTGTG 
GAGATTCTGG CTAACTTGAA GAACATCATC ATTCTGGATT GTGACAAAGA GGCACTTTAT 
AAAAAGGCAG TGTCCATCGC TGATAAGGAG GTGTTTGATT TCCGCATGGT GAACCATGTC 
GATGCAACGG AGGGTGACGC TTACACAGAC ATGCACATTG TGGACACTAG CGTAACCCTG 
ACCGTGGGCT GCATTCAAGT TATTTTCCTC AACAAGTTTA TGTCCTCAAT ACTGGCATTT 
ATTAATAATT TCCAGGAGGC TAAAAATGCA TTGGCGGAGG TGACGGCCCA GGCAGCAGAG 
AAAGCAGCCA CGGGGGTTCT GGAGCTGGCA GAGAGAAGTA CTCGGATCTC CCTGGACGTC 
GACATCAAAG CTCCTGTTGT CTTCCTCCCA CAATCCTCAG TTTCCCACAA CGTCATTGTG 
GCAGATCTGG GTTTAGTGAC TGTAAAGAAC CGCTTTGAGA TCGTCTCCTC TCCAACACAC 
ACCAAGATCC CTCCTGTCAT GGACATCATG GCTGTCGGAT TGAGTGATCT GAAAATGTAC 
AGGACAACGT TCACTGATGA CAAGTTTAAC AGCGAGATTC AGTTGCTCAA GCCAGTCAAC 
ATGGACCTGT CAATCAAACG CAATCTCTCC GCCTCCTGGT ATCACAGCAT ACCCGACATC 
CAGATCAATG CTCACCTCAA GCCCATGAGC CTGATCTTGA GTCAGGAGGA TCTGACTGTG 
GTGCTGAAGA CTCTGAGTGA GAATCTGTCC GAGACGTCTG ATGCTCCTCC AGCTCCGTCC 
AGCTCTGATA AAGGAGATAC GGTTTCTAAC AAAGGCTCCC AGGGTTCTGG AGGCAACACC 
GTTGTGACCG CAGCAGTGGT GGAGGGTCAG AAATCAGGCA AACTGAAGAA CACCCTAAAA 
CTGAACTTCA AGTTTGACTC CATGACGCTG GTCCTCTACA GCCCGAACAG CGAGATCCAG 
TTGTCCGACT CTCGTGATGA GAGTTTGAAG TTGGCCGAGT TCACACTGGG CACCATCTCC 
ACCAGTGTGC ACATGTTCTC CGACAGCTCC ATGAAGGCCT CGCTCAAACT CACCAACTGT 
CTGCTGGATG ACAAGAGGCA GGGAGTGAAG ATGATTACAT CCAGGATGAT GGCGATGCGT 
CAGGACCGAG AGGACAGTGT GATGGTGGAC GTCAGCTACC GCCAGAGTCG GGACGGGACT 
CGTCTGGACA CCCTCGTGCA AGACCTTTAT CTTTGTGCCA GTATGGAGTT TCTGCTCACT 
GTAGCTGATG TGTTTCTTAA AGCTCTGTCT GACGGCTTCT CCGGTCCTCC AAAGAGTAAC 
AAACAACCGG CCAGCACCTC TAAAGACCCA GCGGCTCCTG CTCCACCAGT GGTTGCTAAA 
ACCGAGATGA ATGTGGTGGT GAAGAACCCA GAGATCGTGT TTGTGGCGGA TCTGACGCGT 
GCTGAAGCTC CGGCTCTGGT CATGACCACT CAGTGTGAGC TGGTGATGAA GAGCGAGCCC 
AGCGGTCAGT GCATGACGGC CGCCATTTCA GACCTGAAGA TTGTGGCCTG TCCGTTCCTG 
CGGGAGAAGA GGGAGAACAA CGTGACGACG GTCCTACAGC CCTGCGAGGT TTTCTTCCAG 
AGCCGACAGT CAAACACTGA GCCGCAGTGC ATCGAGCTGT CCATCAGCGC ACTCACGCTC 
AAGGTGTCTC CGATCATCAT AAACACGGTC ATCACCATTC AGACGGCTCT AAGTCCTGCC 
GCTGAGACGC CGCAGGAGCT GGAGAGCCCC ATCCCACAGG ACCTATGGGA GCGCCGCAGC 
CTTCGCGACC TGAAGCTCTG GTTCCTGGAG GAGGAGGGCG AGGAGGTCAG CACGTCCGTC 
ACGCGTCTCA TTCCTCAGGG AGAGTCTCTG AAGATGAGCA TCGCCTCCAT CTGCCTGACG 
CTGGAGGCCG GAGTGGGACA CCGCACCGTC CCCATGCTGC TGGCCAAAGC CTCGTTCCAG 
GGGGACGTCA AGGACTGGAG CACTCTCATC AACCTGCACT GCCACCTCAG CATGGAGGTG 
AACTACTATA ATGAGATGAT GGGTTTATGG GAGCCGCTGT TGGAGCCGCT GGACGATGAG 
GATAAAGACG GTTTTAGACC CTGGACTCTC GCACTGAAGA TGAAGAAGAA GCCGGTGGTC 
AGTTCTGGGG TGTCTGATGA GGTGGACGGC GCTGTGCCTG ATTATAAAAC GGTCATCAGC 
TTCACTAGTA AAGACCAGCT TAACGTCACC CTGTCCAAGT GTGGTCTGCA CATGCTCAGT 
AATCTGGGAA CGGCTTTTGC AGAGGCCGCT AAACAGACGG CCGAGTGCTT TCAGACAGAT 
CAGACACCAT TCGTGGTGAA GAATCGCCTG GGTTTGGCGG TGTCTGTGCT GCACAGCGAA 
GTGTTTCGAC CCATCGGGAA GGAGTCTAAG GATCGTGTGG TGGAGCTCCC GGATGGAGAG 
AGTCTGAATT TGGACTATAC CCTCACCGCC GAGTCCAGCG ACCAGTTCTC TGCCATGACC 
AGCCTCAGTG CCAAAAACTA CATACAGCCC ACTCCAGTCG GCCACACGAC CGCAAGTGTG 
ATCCCGCTCA TCAAAACGGG CCGGGGCATG TACAGCGTCA TGCACAACGA GTCCCGCGTC 
ACTCGCTTCT TCGTCTGTCA GATTTACACC GTCGAGGGCA GCAAATATGT CAAGATTCGC 
TCTCCCTTCC AGATTATCAA CCACTTTTCA GTGCCGTTCC GTGTTTATGA AGGATCCGTG 
TTTCTGGGCG AGTCTCTGCC GACAGAGGAG TTCTGTGTGC CGCTGGGATC CTACAGAGCT 
GAGCTGAGTC TGCAGCCTGT GCCTGATGGC GGTGAAGAGT ACGAGCACTC CGAGAGCTTC 
ACCTACACTG AGGTTGTCCA GAAACCCGGA AAGTGTCTGG AGAAAAACTG CCATCTGCGT 
GGTGACAGCG CCCAGATTCT GAAGGTCAAC ATGGTTCCTC TTGCGGACAC AGTGACGTCC 
CACAGCAGCG AGGAGGAGTT CGATAAGCCC ATCGTACTGC ATTTGTGGCC CACCGTCCTG 
CTGCGCAACC TGCTGCCCTA CCCAATTACT TTCAAGCTAA AGGGCATCGG AGATTCCCCC 
TCGGTGTCGC TGAAGGAGGG CCACTCATCC CAGATCCACA CCGCCATCAT GGACTTCTCC 
GTGTTGGATC TGAGGCTGCT GGGATACTTG CAGCAGGACT GGGAGTCTCA GTACAGCCTC 
AGCAGTGAAC AAGACGAGAT CAGCTTCATT AAGTTTCGCT CGCTCGCACG TGAGGAGGAG 
ACTGAGAGCC GGCCGGAGCT GGACATCGCT ATGCATGTGA AGAAGGAGGA GGGTCAGGTT 
GTGCTGGCCG TACACTCGCC GTACTGGATG GTGAATAAAA CAAGCCGACT GCTGCAGTAC 
AAGGCGGATG ATATTCATCG CAAACACCCC AAGGATTACG ACATGCCGCT GCTCTTCTCC 
TTCAAGCCAC GCAACTTCCT GCAAAACAAC AAGATCCGCC TGATGATCTC AGAAAGTGAG 
CTGTCAGATG ATTTCTCCAT CGACACTGTC GGGAGTTACG GAGATGTCAA ATGCAAAGGC 
AGACAGAAGG ATTACTTGGT GGGAGTGAAG ATCGATGCTA GCAGCTTCAG CTTGACACGT 
TTGGTGACTT TTCTGCCATT CTACATGCTG GTGAACAGAA CCAAACATGT GATTCGGGTC 
TGTGAGGAGG GGCAGGAGAA CTGGACAGAA GCCCGACCCG GACAGGCTGC TGTTCCATTC 
TGGCCAGAAA GAGACTCAAA GAAGCTGAGA GTGAAGGTGG AGGGCAGTCA GTCGTCACCA 
CGCGCCTTTG ACTTCCACCA GCCAGAGAAC TGCCTCCTGC TGCATCTGGA CAAAAGCTTG 
GGTGGCATCA TTGTGGATGT GAATCTGACA GAGCATTCAG CTGTGATCCT CTTCTCTGAT 
TATCATGATG GAGCCGCTCC CTTCCTCCTC ATCAACCACA CTAAGGACGA GACGCTGCAG 
TACCACCAGA GCAACAGTAA GAGGAAGCCT TCGACGTGGT TTCAGGATGA AATACTGGAT 
TTATCAATGA GGGATGAGAC TGATTCCCTC TGCTCTCAGG CCGAGATCGA TGAACTGAGT 
CCAGGTCAGG CGGTTTACTA CACCTGGGCT GAACCAACCG GCTCCAGGAT CCTCAGCTGG 
AAACTGGGCA AATACAAAGG AGAGCTCAAG AGTGAAGAGG ATCAGTTGCT GGATGTGGAC 
AAGGAGGGCA AGTTGTTTTT GGTGTCGTTT TATGAAGGCC TCCAGCGTGT GGCGCTCTTC 
ACCAGCGAGC AGCGCATCTT CAAGAAGATC TGCGACAGTG AGAAGATAGA GCTGGCTCAG 
CAAGAAATCA GCATCTTGCT GCAGAACATG GGCATTTCGC TGGTCAACAA CAACTCCAGC 
CAGGAAGTGG CCTTCATTGG CATCACCAGC TCTGATGTTG TGTGGGAAAC CAAGCCGAAG 
AAGCGCAGCC GCTGGAAAGT TCTGAGTGTT AAAGAGGCAG CAGTTCTGGA GCAGCACTAC 
AGAGACTACA TGGAGAACGG CCCAGTGGAC AGCACCATCA TAGACCTGGA CAACGACTAC 
CAGGTGTTGT TTGCTGCTAA CGGTGTGGAC ATGAGGATCA TGCAGCCCTA CGATGCTCCA 
CTCAGAAGGA ACTTCCTGCC GGCGTTGAAG GTGGAGTACA GCGTTTCAGC CCGCCAGAAA 
ACATACAGAG TCCAGATCAA CAGGATCCAG ATCCAAAACC AGCTGCAAGG AGCCATATTC 
CCGTTTGTGT TTTACCCCAT CAAACCGCCA AAGTCTGTCA GCATGGACTC AGAGCCAAAA 
CCGCTGGCTG ACGTGAGCAT CATCACCAGA GCAGCAGGAC ACTCCGACAT TCTGCGCATC 
AAATATTTTA TGGTGTTGAT TCAGGAGATG GACCTGCGGC TGGATCTGGG CTTTCTGTAT 
GCCATACTTG AGCTCTTCAC TGCTGAACAC ACCAGTGCTG TGACGTCTGA ACAGGAGGTG 
GAGTTGTTTG ATAAAGACAT AGAGTATCTA AAGACTGAGT TAAATCATGT TTCAGCTACA 
GACACATCTC CCATCAGCCT CTACGAGTAC TTCCACATCT CTCCTATAAA GCTTCACTTG 
AGCTTGTCTC TGAGCAGTGG TGGTGAAGAC AGCAATAAGC AAGAGCGCGA GACGGAGCTG 
ATCCCAGTTC AGTCTCTCCA TCTGCTGCTC AAGAGCATCG GGGCTACACT TACAGACGCA 
CAGGACGTCC TCTTTAAACT GGGCTTCTTC GAGCTGAGGT ATCAGTTTCG CACCACTCAG 
CAGCTGCAGT GGGAGGTCGT TCGCCATTAC TCCAAACAGG CTATCAAGCA GATGTATGTC 
CTTGTTCTGG GTTTGGACGT TCTTGGGAAT CCATTCGGGC TGATCCGGGG CCTGTCTGAA 
GGCGTCGAGG CTTTCTTTTA CGAGCCCTAT CAGGGTGCAA TTCAGGGTCC AGAGGAGTTC 
ATGGAGGGCA TGGCTCTCGG CGTGAAGGCT CTGGTTGGAG GCGCTGTGGG TGGTCTGGCC 
GGAGCGGCGT CTCGCATCAC TGGAGCGATG GCTAAAGGTG TGGCGGCGAT GACGATGGAT 
GAAGAGTACC AGCAGAAGAG ACGAGAGGCC ATGAACAAAC AGCCCAGCGG ACTGAGAGAA 
GGACTGACAC GAGGAGGAAA AGGACTGGTG TCTGGCTTCG TGAGTGGCAT CACGGGCATT 
GTGACCAAAC CCATTAAAGG AGCTCAGAAG GAAGGAGCGG CAGGTTTCTT CAAGGGTATG 
GGTAAGGGTT TAGTTGGAGC TCTGACCAGA CCAACAGGGG GCATCATTGA CATGGCCAGC 
AGCACCTTCC AGGGCATCAA GAGAGCTGCA GAGCTGTCTC AGGATGTGGA GAGTCTGCGC 
CCTCCACGCT TCATCCACGA GGACGGAGTG ATCCGGCCGT ACAGAGAGAG AGAAGGCCTC 
GGCAGTCAGA TGCTGCAGAG CAGAACTATC GTCTCTCAGT GGAAAACTAC TGATGAGGAC 
TCTGATGAGG ACCAGGAAAA TCAGCTTAAT GACTTCTATT TTCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302585.1
CDS	152..9619
Translation	MVFESVVVDVLNRFLGDYVGNLDSSQLSLGIWGGDAILRNLEIKENALSQLDIPFKVRAGHIGRLELKIPWKNLYTQSVEATLDEVYLLIVPTASIKYDAEKEERQLQESRQRELQRIEETKQKAAEKENPKGEKQDTFVEKLVTQVIKNLQVKISNIHVRYEDDVTNPSVPLSFGISLQNLSLQTADQNWNPCLLDEKAKLFFKLVRLDNLFAYWNVSSELYSNHNPEEALRYLKHSVAIQTAIPQDYHFIFRPISANAKLRMNPRSDVDFSSPKMDLVVNLPEVAVELSRPQYVSILELLGSVDMMTRNLPYRKYRPYVVVHTNARQWWRYVITGVLEVNVKPRLHMWSWLHIRRHRQHVKKYRELYKNKIISKKPAESLLKELEETERTLDIFNITMARQQAEMEASKAGLRIYRPGVKVEEEQSQGWFGWMWNWSGEASATQTKDVKTGGFDGLMTPAEKAKLYAAIGYSETAVNPNLPKNFENMKMNFHLLRLSLSIKESREKAEIIKLSVIDLKAMLTQRPAAQAIKISAQLSSFEVSGLPREKSAPILLSPRHVDGMKDKSLLNLMFETNPLDARADQRLRIESQPLEIIYDAMTVNSMSAFFMPPKDLQLDELTNATLMKLEQFRDKTSTGLMYIIETQKVLDLKINLMASYVIVPENGFYDPKHNLAILDLGHFQMSSQSRADLPQLSAGSSTLEDIMSRAYDSFDVQLSSLQFLYSKAGGDWKRARPLRQSAFHILEPVDLKVVFSRAMVVTDSRMPKFKMHGELPLLSLRISDDKVRGVLALLSSIPLPESRPTPQSRNTQATPKASKRGNTPQLTPRRPAIDDLRASIIYGSEEELFFDAPSSPIEDLPFFEASSDSRHASAADKRLSMVLKDPKKNMTEFLLSFEINKLSVQLCRVSNRQEVSVLHLFIDGLGTELKLRTFDMSSYTFLREICLECSEYLDSEDKKVHLITTLDNTEEDLLTLEYVKADKNAPDFKAMYKNTEQLIKVNFSSLDIHLHTEALLNSMNFLNNLLPPMKKETQEEQPIIPKEEEEEEEEEEEEEEAKVENSAVAKKSSYKKSKFEDVVNLHIRADLNCLKVFIRGEKARISEISIEGLVSEVQMKKKSVEILANLKNIIILDCDKEALYKKAVSIADKEVFDFRMVNHVDATEGDAYTDMHIVDTSVTLTVGCIQVIFLNKFMSSILAFINNFQEAKNALAEVTAQAAEKAATGVLELAERSTRISLDVDIKAPVVFLPQSSVSHNVIVADLGLVTVKNRFEIVSSPTHTKIPPVMDIMAVGLSDLKMYRTTFTDDKFNSEIQLLKPVNMDLSIKRNLSASWYHSIPDIQINAHLKPMSLILSQEDLTVVLKTLSENLSETSDAPPAPSSSDKGDTVSNKGSQGSGGNTVVTAAVVEGQKSGKLKNTLKLNFKFDSMTLVLYSPNSEIQLSDSRDESLKLAEFTLGTISTSVHMFSDSSMKASLKLTNCLLDDKRQGVKMITSRMMAMRQDREDSVMVDVSYRQSRDGTRLDTLVQDLYLCASMEFLLTVADVFLKALSDGFSGPPKSNKQPASTSKDPAAPAPPVVAKTEMNVVVKNPEIVFVADLTRAEAPALVMTTQCELVMKSEPSGQCMTAAISDLKIVACPFLREKRENNVTTVLQPCEVFFQSRQSNTEPQCIELSISALTLKVSPIIINTVITIQTALSPAAETPQELESPIPQDLWERRSLRDLKLWFLEEEGEEVSTSVTRLIPQGESLKMSIASICLTLEAGVGHRTVPMLLAKASFQGDVKDWSTLINLHCHLSMEVNYYNEMMGLWEPLLEPLDDEDKDGFRPWTLALKMKKKPVVSSGVSDEVDGAVPDYKTVISFTSKDQLNVTLSKCGLHMLSNLGTAFAEAAKQTAECFQTDQTPFVVKNRLGLAVSVLHSEVFRPIGKESKDRVVELPDGESLNLDYTLTAESSDQFSAMTSLSAKNYIQPTPVGHTTASVIPLIKTGRGMYSVMHNESRVTRFFVCQIYTVEGSKYVKIRSPFQIINHFSVPFRVYEGSVFLGESLPTEEFCVPLGSYRAELSLQPVPDGGEEYEHSESFTYTEVVQKPGKCLEKNCHLRGDSAQILKVNMVPLADTVTSHSSEEEFDKPIVLHLWPTVLLRNLLPYPITFKLKGIGDSPSVSLKEGHSSQIHTAIMDFSVLDLRLLGYLQQDWESQYSLSSEQDEISFIKFRSLAREEETESRPELDIAMHVKKEEGQVVLAVHSPYWMVNKTSRLLQYKADDIHRKHPKDYDMPLLFSFKPRNFLQNNKIRLMISESELSDDFSIDTVGSYGDVKCKGRQKDYLVGVKIDASSFSLTRLVTFLPFYMLVNRTKHVIRVCEEGQENWTEARPGQAAVPFWPERDSKKLRVKVEGSQSSPRAFDFHQPENCLLLHLDKSLGGIIVDVNLTEHSAVILFSDYHDGAAPFLLINHTKDETLQYHQSNSKRKPSTWFQDEILDLSMRDETDSLCSQAEIDELSPGQAVYYTWAEPTGSRILSWKLGKYKGELKSEEDQLLDVDKEGKLFLVSFYEGLQRVALFTSEQRIFKKICDSEKIELAQQEISILLQNMGISLVNNNSSQEVAFIGITSSDVVWETKPKKRSRWKVLSVKEAAVLEQHYRDYMENGPVDSTIIDLDNDYQVLFAANGVDMRIMQPYDAPLRRNFLPALKVEYSVSARQKTYRVQINRIQIQNQLQGAIFPFVFYPIKPPKSVSMDSEPKPLADVSIITRAAGHSDILRIKYFMVLIQEMDLRLDLGFLYAILELFTAEHTSAVTSEQEVELFDKDIEYLKTELNHVSATDTSPISLYEYFHISPIKLHLSLSLSSGGEDSNKQERETELIPVQSLHLLLKSIGATLTDAQDVLFKLGFFELRYQFRTTQQLQWEVVRHYSKQAIKQMYVLVLGLDVLGNPFGLIRGLSEGVEAFFYEPYQGAIQGPEEFMEGMALGVKALVGGAVGGLAGAASRITGAMAKGVAAMTMDEEYQQKRREAMNKQPSGLREGLTRGGKGLVSGFVSGITGIVTKPIKGAQKEGAAGFFKGMGKGLVGALTRPTGGIIDMASSTFQGIKRAAELSQDVESLRPPRFIHEDGVIRPYREREGLGSQMLQSRTIVSQWKTTDEDSDEDQENQLNDFYFQ

Target ORF information:

RefSeq Version	XM_009304310.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio vacuolar protein sorting 13 homolog A (S. cerevisiae) (vps13a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304310.2

ORF Insert Sequence:

ATGGTGTTTG AGTCGGTGGT GGTCGACGTG CTGAACCGGT TTCTGGGGGA CTATGTTGGA 
AACCTCGACA GCTCGCAGCT CAGCCTCGGC ATATGGGGAG GTGATGCCAT ATTGAGGAAC 
CTCGAGATTA AAGAAAATGC ATTGAGCCAG CTGGACATTC CTTTCAAAGT CAGAGCAGGA 
CACATTGGGC GTTTGGAGTT AAAGATCCCC TGGAAGAACC TGTACACTCA GTCTGTGGAG 
GCCACGCTGG ATGAAGTTTA CCTGCTCATC GTACCTACTG CTAGTATAAA GTATGACGCA 
GAGAAGGAGG AGCGGCAGCT GCAGGAGTCT CGGCAGAGGG AGCTCCAGCG CATCGAGGAG 
ACCAAACAGA AAGCAGCAGA AAAAGAGAAT CCAAAGGGTG AGAAGCAGGA CACATTTGTG 
GAGAAACTGG TAACACAGGT GATCAAAAAC CTGCAGGTCA AAATATCCAA CATTCACGTC 
CGCTATGAGG ATGATGTCAC CAATCCCAGT GTTCCTCTCT CCTTTGGGAT ATCATTACAA 
AACCTCAGTC TCCAGACGGC GGATCAGAAC TGGAACCCCT GTCTTCTGGA TGAGAAAGCC 
AAACTGTTTT TTAAGCTTGT ACGTCTGGAT AATCTGTTTG CGTACTGGAA CGTCAGTTCA 
GAGCTGTACT CCAACCACAA CCCTGAGGAG GCTCTGCGAT ACCTGAAGCA CAGCGTAGCC 
ATCCAGACTG CCATCCCTCA GGATTATCAC TTCATCTTTC GTCCAATATC TGCCAATGCC 
AAGTTGCGGA TGAACCCTCG CTCTGATGTA GACTTCTCCT CACCCAAAAT GGACCTGGTG 
GTCAACCTTC CTGAAGTGGC CGTGGAGCTC AGCCGACCAC AGTACGTCAG CATTCTGGAG 
CTGTTGGGGT CGGTGGATAT GATGACCCGT AACCTTCCTT ACAGGAAGTA TCGGCCATAT 
GTGGTGGTTC ACACTAACGC TCGTCAGTGG TGGCGATATG TGATCACGGG TGTTCTGGAG 
GTCAATGTGA AGCCTCGTCT TCACATGTGG TCTTGGCTGC ATATTCGTCG ACATCGGCAG 
CATGTGAAAA AGTACAGAGA GCTTTATAAG AATAAAATCA TCAGTAAGAA ACCGGCAGAG 
AGTCTGCTGA AGGAGCTGGA GGAGACTGAA AGAACATTAG ACATATTCAA CATCACAATG 
GCGAGACAGC AGGCTGAGAT GGAGGCCTCG AAGGCAGGGC TCAGGATTTA CCGGCCCGGG 
GTGAAGGTGG AGGAGGAACA GTCACAGGGC TGGTTTGGCT GGATGTGGAA CTGGTCTGGA 
GAAGCCAGTG CCACTCAAAC CAAAGACGTC AAAACTGGAG GTTTTGATGG GTTAATGACG 
CCTGCAGAGA AAGCCAAACT ATACGCTGCC ATCGGATACA GCGAAACTGC TGTCAACCCC 
AACCTGCCAA AAAATTTTGA AAACATGAAG ATGAATTTCC ATCTTCTCCG CCTGTCCCTG 
TCCATCAAAG AGAGCCGAGA GAAGGCGGAG ATCATCAAAC TGTCTGTCAT TGACCTGAAG 
GCCATGCTGA CTCAAAGACC CGCGGCTCAA GCTATAAAGA TCTCCGCTCA GCTGTCGTCG 
TTTGAAGTGT CCGGTCTCCC GCGGGAAAAG TCGGCCCCGA TCCTGCTTTC TCCACGTCAT 
GTGGACGGCA TGAAGGATAA AAGTCTGCTG AACCTCATGT TCGAGACCAA CCCGCTGGAC 
GCTCGTGCTG ACCAGAGACT GCGCATCGAG TCCCAGCCGC TGGAGATCAT CTATGATGCA 
ATGACCGTGA ACAGTATGTC CGCTTTCTTC ATGCCTCCTA AAGACCTGCA GCTGGATGAA 
CTGACCAACG CCACACTGAT GAAGCTGGAG CAGTTCAGAG ACAAAACCTC CACAGGCCTC 
ATGTACATCA TTGAGACGCA GAAAGTTCTG GATCTGAAAA TAAACCTCAT GGCCTCGTAT 
GTCATCGTAC CGGAAAACGG CTTTTATGAC CCCAAACACA ACCTTGCCAT TCTAGATCTG 
GGTCATTTTC AGATGTCAAG TCAGAGCCGA GCTGATCTTC CTCAGCTGTC TGCGGGCAGC 
AGTACTCTAG AGGACATCAT GTCTCGAGCG TACGACAGCT TCGACGTCCA GCTCAGCAGT 
CTGCAGTTCC TCTACTCCAA AGCAGGTGGA GACTGGAAGC GGGCTCGTCC GCTAAGACAG 
TCTGCTTTCC ACATCCTGGA GCCAGTGGAC CTGAAGGTGG TCTTTAGCAG AGCGATGGTG 
GTCACAGACT CCAGGATGCC CAAGTTTAAG ATGCACGGTG AGCTGCCTCT GCTGTCTCTG 
CGGATCTCTG ATGATAAAGT GCGTGGCGTT CTGGCTCTGC TCAGCAGTAT TCCTCTACCT 
GAGAGCCGGC CCACGCCTCA GTCCAGAAAC ACGCAGGCCA CTCCAAAGGC CTCCAAGCGA 
GGAAATACCC CTCAGCTCAC GCCCCGAAGG CCAGCTATAG ATGACCTGCG TGCGTCCATC 
ATATACGGCT CAGAGGAGGA GCTGTTCTTT GACGCCCCCA GTTCACCGAT CGAGGATCTG 
CCGTTCTTCG AGGCCTCGTC GGACTCTCGG CATGCTTCAG CAGCTGATAA ACGTCTGTCT 
ATGGTGCTCA AAGACCCCAA GAAGAACATG ACTGAGTTTC TGCTTAGTTT TGAGATCAAC 
AAGCTCTCGG TGCAGCTGTG CCGTGTGTCG AATAGACAGG AAGTGAGTGT TCTGCATCTG 
TTCATCGATG GATTGGGAAC AGAGCTCAAA CTTCGCACGT TTGACATGTC GTCGTACACT 
TTCCTGCGTG AGATCTGCCT CGAGTGCTCA GAATACTTGG ATTCTGAGGA CAAGAAAGTT 
CACCTTATCA CCACGCTGGA CAACACAGAG GAGGATCTGC TCACTCTGGA GTATGTCAAG 
GCAGACAAAA ATGCACCAGA TTTTAAAGCT ATGTACAAAA ATACAGAGCA ACTAATCAAG 
GTGAATTTCT CCTCTCTGGA CATTCACCTG CACACAGAGG CTCTCCTGAA CTCCATGAAC 
TTCCTAAATA ATCTTCTGCC ACCGATGAAG AAGGAGACCC AGGAGGAACA ACCAATAATT 
CCCAAGGAGG AAGAGGAGGA GGAGGAGGAA GAAGAAGAAG AGGAGGAGGC TAAAGTAGAG 
AATTCTGCTG TGGCCAAGAA GTCTTCCTAC AAGAAGTCAA AGTTTGAGGA TGTGGTGAAT 
TTGCACATCC GCGCAGACCT CAACTGTCTG AAGGTGTTCA TACGAGGAGA GAAAGCCAGG 
ATCTCTGAGA TCAGCATAGA GGGACTGGTT TCTGAAGTTC AGATGAAGAA GAAGTCTGTG 
GAGATTCTGG CTAACTTGAA GAACATCATC ATTCTGGATT GTGACAAAGA GGCACTTTAT 
AAAAAGGCAG TGTCCATCGC TGATAAGGAG GTGTTTGATT TCCGCATGGT GAACCATGTC 
GATGCAACGG AGGGTGACGC TTACACAGAC ATGCACATTG TGGACACTAG CGTAACCCTG 
ACCGTGGGCT GCATTCAAGT TATTTTCCTC AACAAGTTTA TGTCCTCAAT ACTGGCATTT 
ATTAATAATT TCCAGGAGGC TAAAAATGCA TTGGCGGAGG TGACGGCCCA GGCAGCAGAG 
AAAGCAGCCA CGGGGGTTCT GGAGCTGGCA GAGAGAAGTA CTCGGATCTC CCTGGACGTC 
GACATCAAAG CTCCTGTTGT CTTCCTCCCA CAATCCTCAG TTTCCCACAA CGTCATTGTG 
GCAGATCTGG GTTTAGTGAC TGTAAAGAAC CGCTTTGAGA TCGTCTCCTC TCCAACACAC 
ACCAAGATCC CTCCTGTCAT GGACATCATG GCTGTCGGAT TGAGTGATCT GAAAATGTAC 
AGGACAACGT TCACTGATGA CAAGTTTAAC AGCGAGATTC AGTTGCTCAA GCCAGTCAAC 
ATGGACCTGT CAATCAAACG CAATCTCTCC GCCTCCTGGT ATCACAGCAT ACCCGACATC 
CAGATCAATG CTCACCTCAA GCCCATGAGC CTGATCTTGA GTCAGGAGGA TCTGACTGTG 
GTGCTGAAGA CTCTGAGTGA GAATCTGTCC GAGACGTCTG ATGCTCCTCC AGCTCCGTCC 
AGCTCTGATA AAGGAGATAC GGTTTCTAAC AAAGGCTCCC AGGGTTCTGG AGGCAACACC 
GTTGTGACCG CAGCAGTGGT GGAGGGTCAG AAATCAGGCA AACTGAAGAA CACCCTAAAA 
CTGAACTTCA AGTTTGACTC CATGACGCTG GTCCTCTACA GCCCGAACAG CGAGATCCAG 
TTGTCCGACT CTCGTGATGA GAGTTTGAAG TTGGCCGAGT TCACACTGGG CACCATCTCC 
ACCAGTGTGC ACATGTTCTC CGACAGCTCC ATGAAGGCCT CGCTCAAACT CACCAACTGT 
CTGCTGGATG ACAAGAGGCA GGGAGTGAAG ATGATTACAT CCAGGATGAT GGCGATGCGT 
CAGGACCGAG AGGACAGTGT GATGGTGGAC GTCAGCTACC GCCAGAGTCG GGACGGGACT 
CGTCTGGACA CCCTCGTGCA AGACCTTTAT CTTTGTGCCA GTATGGAGTT TCTGCTCACT 
GTAGCTGATG TGTTTCTTAA AGCTCTGTCT GACGGCTTCT CCGGTCCTCC AAAGAGTAAC 
AAACAACCGG CCAGCACCTC TAAAGACCCA GCGGCTCCTG CTCCACCAGT GGTTGCTAAA 
ACCGAGATGA ATGTGGTGGT GAAGAACCCA GAGATCGTGT TTGTGGCGGA TCTGACGCGT 
GCTGAAGCTC CGGCTCTGGT CATGACCACT CAGTGTGAGC TGGTGATGAA GAGCGAGCCC 
AGCGGTCAGT GCATGACGGC CGCCATTTCA GACCTGAAGA TTGTGGCCTG TCCGTTCCTG 
CGGGAGAAGA GGGAGAACAA CGTGACGACG GTCCTACAGC CCTGCGAGGT TTTCTTCCAG 
AGCCGACAGT CAAACACTGA GCCGCAGTGC ATCGAGCTGT CCATCAGCGC ACTCACGCTC 
AAGGTGTCTC CGATCATCAT AAACACGGTC ATCACCATTC AGACGGCTCT AAGTCCTGCC 
GCTGAGACGC CGCAGGAGCT GGAGAGCCCC ATCCCACAGG ACCTATGGGA GCGCCGCAGC 
CTTCGCGACC TGAAGCTCTG GTTCCTGGAG GAGGAGGGCG AGGAGGTCAG CACGTCCGTC 
ACGCGTCTCA TTCCTCAGGG AGAGTCTCTG AAGATGAGCA TCGCCTCCAT CTGCCTGACG 
CTGGAGGCCG GAGTGGGACA CCGCACCGTC CCCATGCTGC TGGCCAAAGC CTCGTTCCAG 
GGGGACGTCA AGGACTGGAG CACTCTCATC AACCTGCACT GCCACCTCAG CATGGAGGTG 
AACTACTATA ATGAGATGAT GGGTTTATGG GAGCCGCTGT TGGAGCCGCT GGACGATGAG 
GATAAAGACG GTTTTAGACC CTGGACTCTC GCACTGAAGA TGAAGAAGAA GCCGGTGGTC 
AGTTCTGGGG TGTCTGATGA GGTGGACGGC GCTGTGCCTG ATTATAAAAC GGTCATCAGC 
TTCACTAGTA AAGACCAGCT TAACGTCACC CTGTCCAAGT GTGGTCTGCA CATGCTCAGT 
AATCTGGGAA CGGCTTTTGC AGAGGCCGCT AAACAGACGG CCGAGTGCTT TCAGACAGAT 
CAGACACCAT TCGTGGTGAA GAATCGCCTG GGTTTGGCGG TGTCTGTGCT GCACAGCGAA 
GTGTTTCGAC CCATCGGGAA GGAGTCTAAG GATCGTGTGG TGGAGCTCCC GGATGGAGAG 
AGTCTGAATT TGGACTATAC CCTCACCGCC GAGTCCAGCG ACCAGTTCTC TGCCATGACC 
AGCCTCAGTG CCAAAAACTA CATACAGCCC ACTCCAGTCG GCCACACGAC CGCAAGTGTG 
ATCCCGCTCA TCAAAACGGG CCGGGGCATG TACAGCGTCA TGCACAACGA GTCCCGCGTC 
ACTCGCTTCT TCGTCTGTCA GATTTACACC GTCGAGGGCA GCAAATATGT CAAGATTCGC 
TCTCCCTTCC AGATTATCAA CCACTTTTCA GTGCCGTTCC GTGTTTATGA AGGATCCGTG 
TTTCTGGGCG AGTCTCTGCC GACAGAGGAG TTCTGTGTGC CGCTGGGATC CTACAGAGCT 
GAGCTGAGTC TGCAGCCTGT GCCTGATGGC GGTGAAGAGT ACGAGCACTC CGAGAGCTTC 
ACCTACACTG AGGTTGTCCA GAAACCCGGA AAGTGTCTGG AGAAAAACTG CCATCTGCGT 
GGTGACAGCG CCCAGATTCT GAAGGTCAAC ATGGTTCCTC TTGCGGACAC AGTGACGTCC 
CACAGCAGCG AGGAGGAGTT CGATAAGCCC ATCGTACTGC ATTTGTGGCC CACCGTCCTG 
CTGCGCAACC TGCTGCCCTA CCCAATTACT TTCAAGCTAA AGGGCATCGG AGATTCCCCC 
TCGGTGTCGC TGAAGGAGGG CCACTCATCC CAGATCCACA CCGCCATCAT GGACTTCTCC 
GTGTTGGATC TGAGGCTGCT GGGATACTTG CAGCAGGACT GGGAGTCTCA GTACAGCCTC 
AGCAGTGAAC AAGACGAGAT CAGCTTCATT AAGTTTCGCT CGCTCGCACG TGAGGAGGAG 
ACTGAGAGCC GGCCGGAGCT GGACATCGCT ATGCATGTGA AGAAGGAGGA GGGTCAGGTT 
GTGCTGGCCG TACACTCGCC GTACTGGATG GTGAATAAAA CAAGCCGACT GCTGCAGTAC 
AAGGCGGATG ATATTCATCG CAAACACCCC AAGGATTACG ACATGCCGCT GCTCTTCTCC 
TTCAAGCCAC GCAACTTCCT GCAAAACAAC AAGATCCGCC TGATGATCTC AGAAAGTGAG 
CTGTCAGATG ATTTCTCCAT CGACACTGTC GGGAGTTACG GAGATGTCAA ATGCAAAGGC 
AGACAGAAGG ATTACTTGGT GGGAGTGAAG ATCGATGCTA GCAGCTTCAG CTTGACACGT 
TTGGTGACTT TTCTGCCATT CTACATGCTG GTGAACAGAA CCAAACATGT GATTCGGGTC 
TGTGAGGAGG GGCAGGAGAA CTGGACAGAA GCCCGACCCG GACAGGCTGC TGTTCCATTC 
TGGCCAGAAA GAGACTCAAA GAAGCTGAGA GTGAAGGTGG AGGGCAGTCA GTCGTCACCA 
CGCGCCTTTG ACTTCCACCA GCCAGAGAAC TGCCTCCTGC TGCATCTGGA CAAAAGCTTG 
GGTGGCATCA TTGTGGATGT GAATCTGACA GAGCATTCAG CTGTGATCCT CTTCTCTGAT 
TATCATGATG GAGCCGCTCC CTTCCTCCTC ATCAACCACA CTAAGGACGA GACGCTGCAG 
TACCACCAGA GCAACAGTAA GAGGAAGCCT TCGACGTGGT TTCAGGATGA AATACTGGAT 
TTATCAATGA GGGATGAGAC TGATTCCCTC TGCTCTCAGG CCGAGATCGA TGAACTGAGT 
CCAGGTCAGG CGGTTTACTA CACCTGGGCT GAACCAACCG GCTCCAGGAT CCTCAGCTGG 
AAACTGGGCA AATACAAAGG AGAGCTCAAG AGTGAAGAGG ATCAGTTGCT GGATGTGGAC 
AAGGAGGGCA AGTTGTTTTT GGTGTCGTTT TATGAAGGCC TCCAGCGTGT GGCGCTCTTC 
ACCAGCGAGC AGCGCATCTT CAAGAAGATC TGCGACAGTG AGAAGATAGA GCTGGCTCAG 
CAAGAAATCA GCATCTTGCT GCAGAACATG GGCATTTCGC TGGTCAACAA CAACTCCAGC 
CAGGAAGTGG CCTTCATTGG CATCACCAGC TCTGATGTTG TGTGGGAAAC CAAGCCGAAG 
AAGCGCAGCC GCTGGAAAGT TCTGAGTGTT AAAGAGGCAG CAGTTCTGGA GCAGCACTAC 
AGAGACTACA TGGAGAACGG CCCAGTGGAC AGCACCATCA TAGACCTGGA CAACGACTAC 
CAGGTGTTGT TTGCTGCTAA CGGTGTGGAC ATGAGGATCA TGCAGCCCTA CGATGCTCCA 
CTCAGAAGGA ACTTCCTGCC GGCGTTGAAG GTGGAGTACA GCGTTTCAGC CCGCCAGAAA 
ACATACAGAG TCCAGATCAA CAGGATCCAG ATCCAAAACC AGCTGCAAGG AGCCATATTC 
CCGTTTGTGT TTTACCCCAT CAAACCGCCA AAGTCTGTCA GCATGGACTC AGAGCCAAAA 
CCGCTGGCTG ACGTGAGCAT CATCACCAGA GCAGCAGGAC ACTCCGACAT TCTGCGCATC 
AAATATTTTA TGGTGTTGAT TCAGGAGATG GACCTGCGGC TGGATCTGGG CTTTCTGTAT 
GCCATACTTG AGCTCTTCAC TGCTGAACAC ACCAGTGCTG TGACGTCTGA ACAGGAGGTG 
GAGTTGTTTG ATAAAGACAT AGAGTATCTA AAGACTGAGT TAAATCATGT TTCAGCTACA 
GACACATCTC CCATCAGCCT CTACGAGTAC TTCCACATCT CTCCTATAAA GCTTCACTTG 
AGCTTGTCTC TGAGCAGTGG TGGTGAAGAC AGCAATAAGC AAGAGCGCGA GACGGAGCTG 
ATCCCAGTTC AGTCTCTCCA TCTGCTGCTC AAGAGCATCG GGGCTACACT TACAGACGCA 
CAGGACGTCC TCTTTAAACT GGGCTTCTTC GAGCTGAGGT ATCAGTTTCG CACCACTCAG 
CAGCTGCAGT GGGAGGTCGT TCGCCATTAC TCCAAACAGG CTATCAAGCA GATGTATGTC 
CTTGTTCTGG GTTTGGACGT TCTTGGGAAT CCATTCGGGC TGATCCGGGG CCTGTCTGAA 
GGCGTCGAGG CTTTCTTTTA CGAGCCCTAT CAGGGTGCAA TTCAGGGTCC AGAGGAGTTC 
ATGGAGGGCA TGGCTCTCGG CGTGAAGGCT CTGGTTGGAG GCGCTGTGGG TGGTCTGGCC 
GGAGCGGCGT CTCGCATCAC TGGAGCGATG GCTAAAGGTG TGGCGGCGAT GACGATGGAT 
GAAGAGTACC AGCAGAAGAG ACGAGAGGCC ATGAACAAAC AGCCCAGCGG ACTGAGAGAA 
GGACTGACAC GAGGAGGAAA AGGACTGGTG TCTGGCTTCG TGAGTGGCAT CACGGGCATT 
GTGACCAAAC CCATTAAAGG AGCTCAGAAG GAAGGAGCGG CAGGTTTCTT CAAGGGTATG 
GGTAAGGGTT TAGTTGGAGC TCTGACCAGA CCAACAGGGG GCATCATTGA CATGGCCAGC 
AGCACCTTCC AGGGCATCAA GAGAGCTGCA GAGCTGTCTC AGGATGTGGA GAGTCTGCGC 
CCTCCACGCT TCATCCACGA GGACGGAGTG ATCCGGCCGT ACAGAGAGAG AGAAGGCCTC 
GGCAGTCAGA TGCTGCAGAG CAGAACTATC GTCTCTCAGT GGAAAACTAC TGATGAGGAC 
TCTGATGAGG ACCAGGAAAA TCAGCTTAAT GACTTCTATT TTCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS13A ( XM_009304310.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009302585.1 Danio rerio vacuolar protein sorting 13 homolog A (S. cerevisiae) (vps13a), transcript variant X3, mRNA.

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CloneID	ODa40322
Clone ID Related Accession (Same CDS sequence)	XM_009304310.2 , XM_009304310.3
Accession Version	XM_009304310.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	vacuolar protein sorting-associated protein 13A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304310.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGTTTG AGTCGGTGGT GGTCGACGTG CTGAACCGGT TTCTGGGGGA CTATGTTGGA 
AACCTCGACA GCTCGCAGCT CAGCCTCGGC ATATGGGGAG GTGATGCCAT ATTGAGGAAC 
CTCGAGATTA AAGAAAATGC ATTGAGCCAG CTGGACATTC CTTTCAAAGT CAGAGCAGGA 
CACATTGGGC GTTTGGAGTT AAAGATCCCC TGGAAGAACC TGTACACTCA GTCTGTGGAG 
GCCACGCTGG ATGAAGTTTA CCTGCTCATC GTACCTACTG CTAGTATAAA GTATGACGCA 
GAGAAGGAGG AGCGGCAGCT GCAGGAGTCT CGGCAGAGGG AGCTCCAGCG CATCGAGGAG 
ACCAAACAGA AAGCAGCAGA AAAAGAGAAT CCAAAGGGTG AGAAGCAGGA CACATTTGTG 
GAGAAACTGG TAACACAGGT GATCAAAAAC CTGCAGGTCA AAATATCCAA CATTCACGTC 
CGCTATGAGG ATGATGTCAC CAATCCCAGT GTTCCTCTCT CCTTTGGGAT ATCATTACAA 
AACCTCAGTC TCCAGACGGC GGATCAGAAC TGGAACCCCT GTCTTCTGGA TGAGAAAGCC 
AAACTGTTTT TTAAGCTTGT ACGTCTGGAT AATCTGTTTG CGTACTGGAA CGTCAGTTCA 
GAGCTGTACT CCAACCACAA CCCTGAGGAG GCTCTGCGAT ACCTGAAGCA CAGCGTAGCC 
ATCCAGACTG CCATCCCTCA GGATTATCAC TTCATCTTTC GTCCAATATC TGCCAATGCC 
AAGTTGCGGA TGAACCCTCG CTCTGATGTA GACTTCTCCT CACCCAAAAT GGACCTGGTG 
GTCAACCTTC CTGAAGTGGC CGTGGAGCTC AGCCGACCAC AGTACGTCAG CATTCTGGAG 
CTGTTGGGGT CGGTGGATAT GATGACCCGT AACCTTCCTT ACAGGAAGTA TCGGCCATAT 
GTGGTGGTTC ACACTAACGC TCGTCAGTGG TGGCGATATG TGATCACGGG TGTTCTGGAG 
GTCAATGTGA AGCCTCGTCT TCACATGTGG TCTTGGCTGC ATATTCGTCG ACATCGGCAG 
CATGTGAAAA AGTACAGAGA GCTTTATAAG AATAAAATCA TCAGTAAGAA ACCGGCAGAG 
AGTCTGCTGA AGGAGCTGGA GGAGACTGAA AGAACATTAG ACATATTCAA CATCACAATG 
GCGAGACAGC AGGCTGAGAT GGAGGCCTCG AAGGCAGGGC TCAGGATTTA CCGGCCCGGG 
GTGAAGGTGG AGGAGGAACA GTCACAGGGC TGGTTTGGCT GGATGTGGAA CTGGTCTGGA 
GAAGCCAGTG CCACTCAAAC CAAAGACGTC AAAACTGGAG GTTTTGATGG GTTAATGACG 
CCTGCAGAGA AAGCCAAACT ATACGCTGCC ATCGGATACA GCGAAACTGC TGTCAACCCC 
AACCTGCCAA AAAATTTTGA AAACATGAAG ATGAATTTCC ATCTTCTCCG CCTGTCCCTG 
TCCATCAAAG AGAGCCGAGA GAAGGCGGAG ATCATCAAAC TGTCTGTCAT TGACCTGAAG 
GCCATGCTGA CTCAAAGACC CGCGGCTCAA GCTATAAAGA TCTCCGCTCA GCTGTCGTCG 
TTTGAAGTGT CCGGTCTCCC GCGGGAAAAG TCGGCCCCGA TCCTGCTTTC TCCACGTCAT 
GTGGACGGCA TGAAGGATAA AAGTCTGCTG AACCTCATGT TCGAGACCAA CCCGCTGGAC 
GCTCGTGCTG ACCAGAGACT GCGCATCGAG TCCCAGCCGC TGGAGATCAT CTATGATGCA 
ATGACCGTGA ACAGTATGTC CGCTTTCTTC ATGCCTCCTA AAGACCTGCA GCTGGATGAA 
CTGACCAACG CCACACTGAT GAAGCTGGAG CAGTTCAGAG ACAAAACCTC CACAGGCCTC 
ATGTACATCA TTGAGACGCA GAAAGTTCTG GATCTGAAAA TAAACCTCAT GGCCTCGTAT 
GTCATCGTAC CGGAAAACGG CTTTTATGAC CCCAAACACA ACCTTGCCAT TCTAGATCTG 
GGTCATTTTC AGATGTCAAG TCAGAGCCGA GCTGATCTTC CTCAGCTGTC TGCGGGCAGC 
AGTACTCTAG AGGACATCAT GTCTCGAGCG TACGACAGCT TCGACGTCCA GCTCAGCAGT 
CTGCAGTTCC TCTACTCCAA AGCAGGTGGA GACTGGAAGC GGGCTCGTCC GCTAAGACAG 
TCTGCTTTCC ACATCCTGGA GCCAGTGGAC CTGAAGGTGG TCTTTAGCAG AGCGATGGTG 
GTCACAGACT CCAGGATGCC CAAGTTTAAG ATGCACGGTG AGCTGCCTCT GCTGTCTCTG 
CGGATCTCTG ATGATAAAGT GCGTGGCGTT CTGGCTCTGC TCAGCAGTAT TCCTCTACCT 
GAGAGCCGGC CCACGCCTCA GTCCAGAAAC ACGCAGGCCA CTCCAAAGGC CTCCAAGCGA 
GGAAATACCC CTCAGCTCAC GCCCCGAAGG CCAGCTATAG ATGACCTGCG TGCGTCCATC 
ATATACGGCT CAGAGGAGGA GCTGTTCTTT GACGCCCCCA GTTCACCGAT CGAGGATCTG 
CCGTTCTTCG AGGCCTCGTC GGACTCTCGG CATGCTTCAG CAGCTGATAA ACGTCTGTCT 
ATGGTGCTCA AAGACCCCAA GAAGAACATG ACTGAGTTTC TGCTTAGTTT TGAGATCAAC 
AAGCTCTCGG TGCAGCTGTG CCGTGTGTCG AATAGACAGG AAGTGAGTGT TCTGCATCTG 
TTCATCGATG GATTGGGAAC AGAGCTCAAA CTTCGCACGT TTGACATGTC GTCGTACACT 
TTCCTGCGTG AGATCTGCCT CGAGTGCTCA GAATACTTGG ATTCTGAGGA CAAGAAAGTT 
CACCTTATCA CCACGCTGGA CAACACAGAG GAGGATCTGC TCACTCTGGA GTATGTCAAG 
GCAGACAAAA ATGCACCAGA TTTTAAAGCT ATGTACAAAA ATACAGAGCA ACTAATCAAG 
GTGAATTTCT CCTCTCTGGA CATTCACCTG CACACAGAGG CTCTCCTGAA CTCCATGAAC 
TTCCTAAATA ATCTTCTGCC ACCGATGAAG AAGGAGACCC AGGAGGAACA ACCAATAATT 
CCCAAGGAGG AAGAGGAGGA GGAGGAGGAA GAAGAAGAAG AGGAGGAGGC TAAAGTAGAG 
AATTCTGCTG TGGCCAAGAA GTCTTCCTAC AAGAAGTCAA AGTTTGAGGA TGTGGTGAAT 
TTGCACATCC GCGCAGACCT CAACTGTCTG AAGGTGTTCA TACGAGGAGA GAAAGCCAGG 
ATCTCTGAGA TCAGCATAGA GGGACTGGTT TCTGAAGTTC AGATGAAGAA GAAGTCTGTG 
GAGATTCTGG CTAACTTGAA GAACATCATC ATTCTGGATT GTGACAAAGA GGCACTTTAT 
AAAAAGGCAG TGTCCATCGC TGATAAGGAG GTGTTTGATT TCCGCATGGT GAACCATGTC 
GATGCAACGG AGGGTGACGC TTACACAGAC ATGCACATTG TGGACACTAG CGTAACCCTG 
ACCGTGGGCT GCATTCAAGT TATTTTCCTC AACAAGTTTA TGTCCTCAAT ACTGGCATTT 
ATTAATAATT TCCAGGAGGC TAAAAATGCA TTGGCGGAGG TGACGGCCCA GGCAGCAGAG 
AAAGCAGCCA CGGGGGTTCT GGAGCTGGCA GAGAGAAGTA CTCGGATCTC CCTGGACGTC 
GACATCAAAG CTCCTGTTGT CTTCCTCCCA CAATCCTCAG TTTCCCACAA CGTCATTGTG 
GCAGATCTGG GTTTAGTGAC TGTAAAGAAC CGCTTTGAGA TCGTCTCCTC TCCAACACAC 
ACCAAGATCC CTCCTGTCAT GGACATCATG GCTGTCGGAT TGAGTGATCT GAAAATGTAC 
AGGACAACGT TCACTGATGA CAAGTTTAAC AGCGAGATTC AGTTGCTCAA GCCAGTCAAC 
ATGGACCTGT CAATCAAACG CAATCTCTCC GCCTCCTGGT ATCACAGCAT ACCCGACATC 
CAGATCAATG CTCACCTCAA GCCCATGAGC CTGATCTTGA GTCAGGAGGA TCTGACTGTG 
GTGCTGAAGA CTCTGAGTGA GAATCTGTCC GAGACGTCTG ATGCTCCTCC AGCTCCGTCC 
AGCTCTGATA AAGGAGATAC GGTTTCTAAC AAAGGCTCCC AGGGTTCTGG AGGCAACACC 
GTTGTGACCG CAGCAGTGGT GGAGGGTCAG AAATCAGGCA AACTGAAGAA CACCCTAAAA 
CTGAACTTCA AGTTTGACTC CATGACGCTG GTCCTCTACA GCCCGAACAG CGAGATCCAG 
TTGTCCGACT CTCGTGATGA GAGTTTGAAG TTGGCCGAGT TCACACTGGG CACCATCTCC 
ACCAGTGTGC ACATGTTCTC CGACAGCTCC ATGAAGGCCT CGCTCAAACT CACCAACTGT 
CTGCTGGATG ACAAGAGGCA GGGAGTGAAG ATGATTACAT CCAGGATGAT GGCGATGCGT 
CAGGACCGAG AGGACAGTGT GATGGTGGAC GTCAGCTACC GCCAGAGTCG GGACGGGACT 
CGTCTGGACA CCCTCGTGCA AGACCTTTAT CTTTGTGCCA GTATGGAGTT TCTGCTCACT 
GTAGCTGATG TGTTTCTTAA AGCTCTGTCT GACGGCTTCT CCGGTCCTCC AAAGAGTAAC 
AAACAACCGG CCAGCACCTC TAAAGACCCA GCGGCTCCTG CTCCACCAGT GGTTGCTAAA 
ACCGAGATGA ATGTGGTGGT GAAGAACCCA GAGATCGTGT TTGTGGCGGA TCTGACGCGT 
GCTGAAGCTC CGGCTCTGGT CATGACCACT CAGTGTGAGC TGGTGATGAA GAGCGAGCCC 
AGCGGTCAGT GCATGACGGC CGCCATTTCA GACCTGAAGA TTGTGGCCTG TCCGTTCCTG 
CGGGAGAAGA GGGAGAACAA CGTGACGACG GTCCTACAGC CCTGCGAGGT TTTCTTCCAG 
AGCCGACAGT CAAACACTGA GCCGCAGTGC ATCGAGCTGT CCATCAGCGC ACTCACGCTC 
AAGGTGTCTC CGATCATCAT AAACACGGTC ATCACCATTC AGACGGCTCT AAGTCCTGCC 
GCTGAGACGC CGCAGGAGCT GGAGAGCCCC ATCCCACAGG ACCTATGGGA GCGCCGCAGC 
CTTCGCGACC TGAAGCTCTG GTTCCTGGAG GAGGAGGGCG AGGAGGTCAG CACGTCCGTC 
ACGCGTCTCA TTCCTCAGGG AGAGTCTCTG AAGATGAGCA TCGCCTCCAT CTGCCTGACG 
CTGGAGGCCG GAGTGGGACA CCGCACCGTC CCCATGCTGC TGGCCAAAGC CTCGTTCCAG 
GGGGACGTCA AGGACTGGAG CACTCTCATC AACCTGCACT GCCACCTCAG CATGGAGGTG 
AACTACTATA ATGAGATGAT GGGTTTATGG GAGCCGCTGT TGGAGCCGCT GGACGATGAG 
GATAAAGACG GTTTTAGACC CTGGACTCTC GCACTGAAGA TGAAGAAGAA GCCGGTGGTC 
AGTTCTGGGG TGTCTGATGA GGTGGACGGC GCTGTGCCTG ATTATAAAAC GGTCATCAGC 
TTCACTAGTA AAGACCAGCT TAACGTCACC CTGTCCAAGT GTGGTCTGCA CATGCTCAGT 
AATCTGGGAA CGGCTTTTGC AGAGGCCGCT AAACAGACGG CCGAGTGCTT TCAGACAGAT 
CAGACACCAT TCGTGGTGAA GAATCGCCTG GGTTTGGCGG TGTCTGTGCT GCACAGCGAA 
GTGTTTCGAC CCATCGGGAA GGAGTCTAAG GATCGTGTGG TGGAGCTCCC GGATGGAGAG 
AGTCTGAATT TGGACTATAC CCTCACCGCC GAGTCCAGCG ACCAGTTCTC TGCCATGACC 
AGCCTCAGTG CCAAAAACTA CATACAGCCC ACTCCAGTCG GCCACACGAC CGCAAGTGTG 
ATCCCGCTCA TCAAAACGGG CCGGGGCATG TACAGCGTCA TGCACAACGA GTCCCGCGTC 
ACTCGCTTCT TCGTCTGTCA GATTTACACC GTCGAGGGCA GCAAATATGT CAAGATTCGC 
TCTCCCTTCC AGATTATCAA CCACTTTTCA GTGCCGTTCC GTGTTTATGA AGGATCCGTG 
TTTCTGGGCG AGTCTCTGCC GACAGAGGAG TTCTGTGTGC CGCTGGGATC CTACAGAGCT 
GAGCTGAGTC TGCAGCCTGT GCCTGATGGC GGTGAAGAGT ACGAGCACTC CGAGAGCTTC 
ACCTACACTG AGGTTGTCCA GAAACCCGGA AAGTGTCTGG AGAAAAACTG CCATCTGCGT 
GGTGACAGCG CCCAGATTCT GAAGGTCAAC ATGGTTCCTC TTGCGGACAC AGTGACGTCC 
CACAGCAGCG AGGAGGAGTT CGATAAGCCC ATCGTACTGC ATTTGTGGCC CACCGTCCTG 
CTGCGCAACC TGCTGCCCTA CCCAATTACT TTCAAGCTAA AGGGCATCGG AGATTCCCCC 
TCGGTGTCGC TGAAGGAGGG CCACTCATCC CAGATCCACA CCGCCATCAT GGACTTCTCC 
GTGTTGGATC TGAGGCTGCT GGGATACTTG CAGCAGGACT GGGAGTCTCA GTACAGCCTC 
AGCAGTGAAC AAGACGAGAT CAGCTTCATT AAGTTTCGCT CGCTCGCACG TGAGGAGGAG 
ACTGAGAGCC GGCCGGAGCT GGACATCGCT ATGCATGTGA AGAAGGAGGA GGGTCAGGTT 
GTGCTGGCCG TACACTCGCC GTACTGGATG GTGAATAAAA CAAGCCGACT GCTGCAGTAC 
AAGGCGGATG ATATTCATCG CAAACACCCC AAGGATTACG ACATGCCGCT GCTCTTCTCC 
TTCAAGCCAC GCAACTTCCT GCAAAACAAC AAGATCCGCC TGATGATCTC AGAAAGTGAG 
CTGTCAGATG ATTTCTCCAT CGACACTGTC GGGAGTTACG GAGATGTCAA ATGCAAAGGC 
AGACAGAAGG ATTACTTGGT GGGAGTGAAG ATCGATGCTA GCAGCTTCAG CTTGACACGT 
TTGGTGACTT TTCTGCCATT CTACATGCTG GTGAACAGAA CCAAACATGT GATTCGGGTC 
TGTGAGGAGG GGCAGGAGAA CTGGACAGAA GCCCGACCCG GACAGGCTGC TGTTCCATTC 
TGGCCAGAAA GAGACTCAAA GAAGCTGAGA GTGAAGGTGG AGGGCAGTCA GTCGTCACCA 
CGCGCCTTTG ACTTCCACCA GCCAGAGAAC TGCCTCCTGC TGCATCTGGA CAAAAGCTTG 
GGTGGCATCA TTGTGGATGT GAATCTGACA GAGCATTCAG CTGTGATCCT CTTCTCTGAT 
TATCATGATG GAGCCGCTCC CTTCCTCCTC ATCAACCACA CTAAGGACGA GACGCTGCAG 
TACCACCAGA GCAACAGTAA GAGGAAGCCT TCGACGTGGT TTCAGGATGA AATACTGGAT 
TTATCAATGA GGGATGAGAC TGATTCCCTC TGCTCTCAGG CCGAGATCGA TGAACTGAGT 
CCAGGTCAGG CGGTTTACTA CACCTGGGCT GAACCAACCG GCTCCAGGAT CCTCAGCTGG 
AAACTGGGCA AATACAAAGG AGAGCTCAAG AGTGAAGAGG ATCAGTTGCT GGATGTGGAC 
AAGGAGGGCA AGTTGTTTTT GGTGTCGTTT TATGAAGGCC TCCAGCGTGT GGCGCTCTTC 
ACCAGCGAGC AGCGCATCTT CAAGAAGATC TGCGACAGTG AGAAGATAGA GCTGGCTCAG 
CAAGAAATCA GCATCTTGCT GCAGAACATG GGCATTTCGC TGGTCAACAA CAACTCCAGC 
CAGGAAGTGG CCTTCATTGG CATCACCAGC TCTGATGTTG TGTGGGAAAC CAAGCCGAAG 
AAGCGCAGCC GCTGGAAAGT TCTGAGTGTT AAAGAGGCAG CAGTTCTGGA GCAGCACTAC 
AGAGACTACA TGGAGAACGG CCCAGTGGAC AGCACCATCA TAGACCTGGA CAACGACTAC 
CAGGTGTTGT TTGCTGCTAA CGGTGTGGAC ATGAGGATCA TGCAGCCCTA CGATGCTCCA 
CTCAGAAGGA ACTTCCTGCC GGCGTTGAAG GTGGAGTACA GCGTTTCAGC CCGCCAGAAA 
ACATACAGAG TCCAGATCAA CAGGATCCAG ATCCAAAACC AGCTGCAAGG AGCCATATTC 
CCGTTTGTGT TTTACCCCAT CAAACCGCCA AAGTCTGTCA GCATGGACTC AGAGCCAAAA 
CCGCTGGCTG ACGTGAGCAT CATCACCAGA GCAGCAGGAC ACTCCGACAT TCTGCGCATC 
AAATATTTTA TGGTGTTGAT TCAGGAGATG GACCTGCGGC TGGATCTGGG CTTTCTGTAT 
GCCATACTTG AGCTCTTCAC TGCTGAACAC ACCAGTGCTG TGACGTCTGA ACAGGAGGTG 
GAGTTGTTTG ATAAAGACAT AGAGTATCTA AAGACTGAGT TAAATCATGT TTCAGCTACA 
GACACATCTC CCATCAGCCT CTACGAGTAC TTCCACATCT CTCCTATAAA GCTTCACTTG 
AGCTTGTCTC TGAGCAGTGG TGGTGAAGAC AGCAATAAGC AAGAGCGCGA GACGGAGCTG 
ATCCCAGTTC AGTCTCTCCA TCTGCTGCTC AAGAGCATCG GGGCTACACT TACAGACGCA 
CAGGACGTCC TCTTTAAACT GGGCTTCTTC GAGCTGAGGT ATCAGTTTCG CACCACTCAG 
CAGCTGCAGT GGGAGGTCGT TCGCCATTAC TCCAAACAGG CTATCAAGCA GATGTATGTC 
CTTGTTCTGG GTTTGGACGT TCTTGGGAAT CCATTCGGGC TGATCCGGGG CCTGTCTGAA 
GGCGTCGAGG CTTTCTTTTA CGAGCCCTAT CAGGGTGCAA TTCAGGGTCC AGAGGAGTTC 
ATGGAGGGCA TGGCTCTCGG CGTGAAGGCT CTGGTTGGAG GCGCTGTGGG TGGTCTGGCC 
GGAGCGGCGT CTCGCATCAC TGGAGCGATG GCTAAAGGTG TGGCGGCGAT GACGATGGAT 
GAAGAGTACC AGCAGAAGAG ACGAGAGGCC ATGAACAAAC AGCCCAGCGG ACTGAGAGAA 
GGACTGACAC GAGGAGGAAA AGGACTGGTG TCTGGCTTCG TGAGTGGCAT CACGGGCATT 
GTGACCAAAC CCATTAAAGG AGCTCAGAAG GAAGGAGCGG CAGGTTTCTT CAAGGGTATG 
GGTAAGGGTT TAGTTGGAGC TCTGACCAGA CCAACAGGGG GCATCATTGA CATGGCCAGC 
AGCACCTTCC AGGGCATCAA GAGAGCTGCA GAGCTGTCTC AGGATGTGGA GAGTCTGCGC 
CCTCCACGCT TCATCCACGA GGACGGAGTG ATCCGGCCGT ACAGAGAGAG AGAAGGCCTC 
GGCAGTCAGA TGCTGCAGAG CAGAACTATC GTCTCTCAGT GGAAAACTAC TGATGAGGAC 
TCTGATGAGG ACCAGGAAAA TCAGCTTAAT GACTTCTATT TTCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302585.1
CDS	51..9518
Translation	MVFESVVVDVLNRFLGDYVGNLDSSQLSLGIWGGDAILRNLEIKENALSQLDIPFKVRAGHIGRLELKIPWKNLYTQSVEATLDEVYLLIVPTASIKYDAEKEERQLQESRQRELQRIEETKQKAAEKENPKGEKQDTFVEKLVTQVIKNLQVKISNIHVRYEDDVTNPSVPLSFGISLQNLSLQTADQNWNPCLLDEKAKLFFKLVRLDNLFAYWNVSSELYSNHNPEEALRYLKHSVAIQTAIPQDYHFIFRPISANAKLRMNPRSDVDFSSPKMDLVVNLPEVAVELSRPQYVSILELLGSVDMMTRNLPYRKYRPYVVVHTNARQWWRYVITGVLEVNVKPRLHMWSWLHIRRHRQHVKKYRELYKNKIISKKPAESLLKELEETERTLDIFNITMARQQAEMEASKAGLRIYRPGVKVEEEQSQGWFGWMWNWSGEASATQTKDVKTGGFDGLMTPAEKAKLYAAIGYSETAVNPNLPKNFENMKMNFHLLRLSLSIKESREKAEIIKLSVIDLKAMLTQRPAAQAIKISAQLSSFEVSGLPREKSAPILLSPRHVDGMKDKSLLNLMFETNPLDARADQRLRIESQPLEIIYDAMTVNSMSAFFMPPKDLQLDELTNATLMKLEQFRDKTSTGLMYIIETQKVLDLKINLMASYVIVPENGFYDPKHNLAILDLGHFQMSSQSRADLPQLSAGSSTLEDIMSRAYDSFDVQLSSLQFLYSKAGGDWKRARPLRQSAFHILEPVDLKVVFSRAMVVTDSRMPKFKMHGELPLLSLRISDDKVRGVLALLSSIPLPESRPTPQSRNTQATPKASKRGNTPQLTPRRPAIDDLRASIIYGSEEELFFDAPSSPIEDLPFFEASSDSRHASAADKRLSMVLKDPKKNMTEFLLSFEINKLSVQLCRVSNRQEVSVLHLFIDGLGTELKLRTFDMSSYTFLREICLECSEYLDSEDKKVHLITTLDNTEEDLLTLEYVKADKNAPDFKAMYKNTEQLIKVNFSSLDIHLHTEALLNSMNFLNNLLPPMKKETQEEQPIIPKEEEEEEEEEEEEEEAKVENSAVAKKSSYKKSKFEDVVNLHIRADLNCLKVFIRGEKARISEISIEGLVSEVQMKKKSVEILANLKNIIILDCDKEALYKKAVSIADKEVFDFRMVNHVDATEGDAYTDMHIVDTSVTLTVGCIQVIFLNKFMSSILAFINNFQEAKNALAEVTAQAAEKAATGVLELAERSTRISLDVDIKAPVVFLPQSSVSHNVIVADLGLVTVKNRFEIVSSPTHTKIPPVMDIMAVGLSDLKMYRTTFTDDKFNSEIQLLKPVNMDLSIKRNLSASWYHSIPDIQINAHLKPMSLILSQEDLTVVLKTLSENLSETSDAPPAPSSSDKGDTVSNKGSQGSGGNTVVTAAVVEGQKSGKLKNTLKLNFKFDSMTLVLYSPNSEIQLSDSRDESLKLAEFTLGTISTSVHMFSDSSMKASLKLTNCLLDDKRQGVKMITSRMMAMRQDREDSVMVDVSYRQSRDGTRLDTLVQDLYLCASMEFLLTVADVFLKALSDGFSGPPKSNKQPASTSKDPAAPAPPVVAKTEMNVVVKNPEIVFVADLTRAEAPALVMTTQCELVMKSEPSGQCMTAAISDLKIVACPFLREKRENNVTTVLQPCEVFFQSRQSNTEPQCIELSISALTLKVSPIIINTVITIQTALSPAAETPQELESPIPQDLWERRSLRDLKLWFLEEEGEEVSTSVTRLIPQGESLKMSIASICLTLEAGVGHRTVPMLLAKASFQGDVKDWSTLINLHCHLSMEVNYYNEMMGLWEPLLEPLDDEDKDGFRPWTLALKMKKKPVVSSGVSDEVDGAVPDYKTVISFTSKDQLNVTLSKCGLHMLSNLGTAFAEAAKQTAECFQTDQTPFVVKNRLGLAVSVLHSEVFRPIGKESKDRVVELPDGESLNLDYTLTAESSDQFSAMTSLSAKNYIQPTPVGHTTASVIPLIKTGRGMYSVMHNESRVTRFFVCQIYTVEGSKYVKIRSPFQIINHFSVPFRVYEGSVFLGESLPTEEFCVPLGSYRAELSLQPVPDGGEEYEHSESFTYTEVVQKPGKCLEKNCHLRGDSAQILKVNMVPLADTVTSHSSEEEFDKPIVLHLWPTVLLRNLLPYPITFKLKGIGDSPSVSLKEGHSSQIHTAIMDFSVLDLRLLGYLQQDWESQYSLSSEQDEISFIKFRSLAREEETESRPELDIAMHVKKEEGQVVLAVHSPYWMVNKTSRLLQYKADDIHRKHPKDYDMPLLFSFKPRNFLQNNKIRLMISESELSDDFSIDTVGSYGDVKCKGRQKDYLVGVKIDASSFSLTRLVTFLPFYMLVNRTKHVIRVCEEGQENWTEARPGQAAVPFWPERDSKKLRVKVEGSQSSPRAFDFHQPENCLLLHLDKSLGGIIVDVNLTEHSAVILFSDYHDGAAPFLLINHTKDETLQYHQSNSKRKPSTWFQDEILDLSMRDETDSLCSQAEIDELSPGQAVYYTWAEPTGSRILSWKLGKYKGELKSEEDQLLDVDKEGKLFLVSFYEGLQRVALFTSEQRIFKKICDSEKIELAQQEISILLQNMGISLVNNNSSQEVAFIGITSSDVVWETKPKKRSRWKVLSVKEAAVLEQHYRDYMENGPVDSTIIDLDNDYQVLFAANGVDMRIMQPYDAPLRRNFLPALKVEYSVSARQKTYRVQINRIQIQNQLQGAIFPFVFYPIKPPKSVSMDSEPKPLADVSIITRAAGHSDILRIKYFMVLIQEMDLRLDLGFLYAILELFTAEHTSAVTSEQEVELFDKDIEYLKTELNHVSATDTSPISLYEYFHISPIKLHLSLSLSSGGEDSNKQERETELIPVQSLHLLLKSIGATLTDAQDVLFKLGFFELRYQFRTTQQLQWEVVRHYSKQAIKQMYVLVLGLDVLGNPFGLIRGLSEGVEAFFYEPYQGAIQGPEEFMEGMALGVKALVGGAVGGLAGAASRITGAMAKGVAAMTMDEEYQQKRREAMNKQPSGLREGLTRGGKGLVSGFVSGITGIVTKPIKGAQKEGAAGFFKGMGKGLVGALTRPTGGIIDMASSTFQGIKRAAELSQDVESLRPPRFIHEDGVIRPYREREGLGSQMLQSRTIVSQWKTTDEDSDEDQENQLNDFYFQ

Target ORF information:

RefSeq Version	XM_009304310.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio vacuolar protein sorting 13 homolog A (S. cerevisiae) (vps13a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304310.3

ORF Insert Sequence:

ATGGTGTTTG AGTCGGTGGT GGTCGACGTG CTGAACCGGT TTCTGGGGGA CTATGTTGGA 
AACCTCGACA GCTCGCAGCT CAGCCTCGGC ATATGGGGAG GTGATGCCAT ATTGAGGAAC 
CTCGAGATTA AAGAAAATGC ATTGAGCCAG CTGGACATTC CTTTCAAAGT CAGAGCAGGA 
CACATTGGGC GTTTGGAGTT AAAGATCCCC TGGAAGAACC TGTACACTCA GTCTGTGGAG 
GCCACGCTGG ATGAAGTTTA CCTGCTCATC GTACCTACTG CTAGTATAAA GTATGACGCA 
GAGAAGGAGG AGCGGCAGCT GCAGGAGTCT CGGCAGAGGG AGCTCCAGCG CATCGAGGAG 
ACCAAACAGA AAGCAGCAGA AAAAGAGAAT CCAAAGGGTG AGAAGCAGGA CACATTTGTG 
GAGAAACTGG TAACACAGGT GATCAAAAAC CTGCAGGTCA AAATATCCAA CATTCACGTC 
CGCTATGAGG ATGATGTCAC CAATCCCAGT GTTCCTCTCT CCTTTGGGAT ATCATTACAA 
AACCTCAGTC TCCAGACGGC GGATCAGAAC TGGAACCCCT GTCTTCTGGA TGAGAAAGCC 
AAACTGTTTT TTAAGCTTGT ACGTCTGGAT AATCTGTTTG CGTACTGGAA CGTCAGTTCA 
GAGCTGTACT CCAACCACAA CCCTGAGGAG GCTCTGCGAT ACCTGAAGCA CAGCGTAGCC 
ATCCAGACTG CCATCCCTCA GGATTATCAC TTCATCTTTC GTCCAATATC TGCCAATGCC 
AAGTTGCGGA TGAACCCTCG CTCTGATGTA GACTTCTCCT CACCCAAAAT GGACCTGGTG 
GTCAACCTTC CTGAAGTGGC CGTGGAGCTC AGCCGACCAC AGTACGTCAG CATTCTGGAG 
CTGTTGGGGT CGGTGGATAT GATGACCCGT AACCTTCCTT ACAGGAAGTA TCGGCCATAT 
GTGGTGGTTC ACACTAACGC TCGTCAGTGG TGGCGATATG TGATCACGGG TGTTCTGGAG 
GTCAATGTGA AGCCTCGTCT TCACATGTGG TCTTGGCTGC ATATTCGTCG ACATCGGCAG 
CATGTGAAAA AGTACAGAGA GCTTTATAAG AATAAAATCA TCAGTAAGAA ACCGGCAGAG 
AGTCTGCTGA AGGAGCTGGA GGAGACTGAA AGAACATTAG ACATATTCAA CATCACAATG 
GCGAGACAGC AGGCTGAGAT GGAGGCCTCG AAGGCAGGGC TCAGGATTTA CCGGCCCGGG 
GTGAAGGTGG AGGAGGAACA GTCACAGGGC TGGTTTGGCT GGATGTGGAA CTGGTCTGGA 
GAAGCCAGTG CCACTCAAAC CAAAGACGTC AAAACTGGAG GTTTTGATGG GTTAATGACG 
CCTGCAGAGA AAGCCAAACT ATACGCTGCC ATCGGATACA GCGAAACTGC TGTCAACCCC 
AACCTGCCAA AAAATTTTGA AAACATGAAG ATGAATTTCC ATCTTCTCCG CCTGTCCCTG 
TCCATCAAAG AGAGCCGAGA GAAGGCGGAG ATCATCAAAC TGTCTGTCAT TGACCTGAAG 
GCCATGCTGA CTCAAAGACC CGCGGCTCAA GCTATAAAGA TCTCCGCTCA GCTGTCGTCG 
TTTGAAGTGT CCGGTCTCCC GCGGGAAAAG TCGGCCCCGA TCCTGCTTTC TCCACGTCAT 
GTGGACGGCA TGAAGGATAA AAGTCTGCTG AACCTCATGT TCGAGACCAA CCCGCTGGAC 
GCTCGTGCTG ACCAGAGACT GCGCATCGAG TCCCAGCCGC TGGAGATCAT CTATGATGCA 
ATGACCGTGA ACAGTATGTC CGCTTTCTTC ATGCCTCCTA AAGACCTGCA GCTGGATGAA 
CTGACCAACG CCACACTGAT GAAGCTGGAG CAGTTCAGAG ACAAAACCTC CACAGGCCTC 
ATGTACATCA TTGAGACGCA GAAAGTTCTG GATCTGAAAA TAAACCTCAT GGCCTCGTAT 
GTCATCGTAC CGGAAAACGG CTTTTATGAC CCCAAACACA ACCTTGCCAT TCTAGATCTG 
GGTCATTTTC AGATGTCAAG TCAGAGCCGA GCTGATCTTC CTCAGCTGTC TGCGGGCAGC 
AGTACTCTAG AGGACATCAT GTCTCGAGCG TACGACAGCT TCGACGTCCA GCTCAGCAGT 
CTGCAGTTCC TCTACTCCAA AGCAGGTGGA GACTGGAAGC GGGCTCGTCC GCTAAGACAG 
TCTGCTTTCC ACATCCTGGA GCCAGTGGAC CTGAAGGTGG TCTTTAGCAG AGCGATGGTG 
GTCACAGACT CCAGGATGCC CAAGTTTAAG ATGCACGGTG AGCTGCCTCT GCTGTCTCTG 
CGGATCTCTG ATGATAAAGT GCGTGGCGTT CTGGCTCTGC TCAGCAGTAT TCCTCTACCT 
GAGAGCCGGC CCACGCCTCA GTCCAGAAAC ACGCAGGCCA CTCCAAAGGC CTCCAAGCGA 
GGAAATACCC CTCAGCTCAC GCCCCGAAGG CCAGCTATAG ATGACCTGCG TGCGTCCATC 
ATATACGGCT CAGAGGAGGA GCTGTTCTTT GACGCCCCCA GTTCACCGAT CGAGGATCTG 
CCGTTCTTCG AGGCCTCGTC GGACTCTCGG CATGCTTCAG CAGCTGATAA ACGTCTGTCT 
ATGGTGCTCA AAGACCCCAA GAAGAACATG ACTGAGTTTC TGCTTAGTTT TGAGATCAAC 
AAGCTCTCGG TGCAGCTGTG CCGTGTGTCG AATAGACAGG AAGTGAGTGT TCTGCATCTG 
TTCATCGATG GATTGGGAAC AGAGCTCAAA CTTCGCACGT TTGACATGTC GTCGTACACT 
TTCCTGCGTG AGATCTGCCT CGAGTGCTCA GAATACTTGG ATTCTGAGGA CAAGAAAGTT 
CACCTTATCA CCACGCTGGA CAACACAGAG GAGGATCTGC TCACTCTGGA GTATGTCAAG 
GCAGACAAAA ATGCACCAGA TTTTAAAGCT ATGTACAAAA ATACAGAGCA ACTAATCAAG 
GTGAATTTCT CCTCTCTGGA CATTCACCTG CACACAGAGG CTCTCCTGAA CTCCATGAAC 
TTCCTAAATA ATCTTCTGCC ACCGATGAAG AAGGAGACCC AGGAGGAACA ACCAATAATT 
CCCAAGGAGG AAGAGGAGGA GGAGGAGGAA GAAGAAGAAG AGGAGGAGGC TAAAGTAGAG 
AATTCTGCTG TGGCCAAGAA GTCTTCCTAC AAGAAGTCAA AGTTTGAGGA TGTGGTGAAT 
TTGCACATCC GCGCAGACCT CAACTGTCTG AAGGTGTTCA TACGAGGAGA GAAAGCCAGG 
ATCTCTGAGA TCAGCATAGA GGGACTGGTT TCTGAAGTTC AGATGAAGAA GAAGTCTGTG 
GAGATTCTGG CTAACTTGAA GAACATCATC ATTCTGGATT GTGACAAAGA GGCACTTTAT 
AAAAAGGCAG TGTCCATCGC TGATAAGGAG GTGTTTGATT TCCGCATGGT GAACCATGTC 
GATGCAACGG AGGGTGACGC TTACACAGAC ATGCACATTG TGGACACTAG CGTAACCCTG 
ACCGTGGGCT GCATTCAAGT TATTTTCCTC AACAAGTTTA TGTCCTCAAT ACTGGCATTT 
ATTAATAATT TCCAGGAGGC TAAAAATGCA TTGGCGGAGG TGACGGCCCA GGCAGCAGAG 
AAAGCAGCCA CGGGGGTTCT GGAGCTGGCA GAGAGAAGTA CTCGGATCTC CCTGGACGTC 
GACATCAAAG CTCCTGTTGT CTTCCTCCCA CAATCCTCAG TTTCCCACAA CGTCATTGTG 
GCAGATCTGG GTTTAGTGAC TGTAAAGAAC CGCTTTGAGA TCGTCTCCTC TCCAACACAC 
ACCAAGATCC CTCCTGTCAT GGACATCATG GCTGTCGGAT TGAGTGATCT GAAAATGTAC 
AGGACAACGT TCACTGATGA CAAGTTTAAC AGCGAGATTC AGTTGCTCAA GCCAGTCAAC 
ATGGACCTGT CAATCAAACG CAATCTCTCC GCCTCCTGGT ATCACAGCAT ACCCGACATC 
CAGATCAATG CTCACCTCAA GCCCATGAGC CTGATCTTGA GTCAGGAGGA TCTGACTGTG 
GTGCTGAAGA CTCTGAGTGA GAATCTGTCC GAGACGTCTG ATGCTCCTCC AGCTCCGTCC 
AGCTCTGATA AAGGAGATAC GGTTTCTAAC AAAGGCTCCC AGGGTTCTGG AGGCAACACC 
GTTGTGACCG CAGCAGTGGT GGAGGGTCAG AAATCAGGCA AACTGAAGAA CACCCTAAAA 
CTGAACTTCA AGTTTGACTC CATGACGCTG GTCCTCTACA GCCCGAACAG CGAGATCCAG 
TTGTCCGACT CTCGTGATGA GAGTTTGAAG TTGGCCGAGT TCACACTGGG CACCATCTCC 
ACCAGTGTGC ACATGTTCTC CGACAGCTCC ATGAAGGCCT CGCTCAAACT CACCAACTGT 
CTGCTGGATG ACAAGAGGCA GGGAGTGAAG ATGATTACAT CCAGGATGAT GGCGATGCGT 
CAGGACCGAG AGGACAGTGT GATGGTGGAC GTCAGCTACC GCCAGAGTCG GGACGGGACT 
CGTCTGGACA CCCTCGTGCA AGACCTTTAT CTTTGTGCCA GTATGGAGTT TCTGCTCACT 
GTAGCTGATG TGTTTCTTAA AGCTCTGTCT GACGGCTTCT CCGGTCCTCC AAAGAGTAAC 
AAACAACCGG CCAGCACCTC TAAAGACCCA GCGGCTCCTG CTCCACCAGT GGTTGCTAAA 
ACCGAGATGA ATGTGGTGGT GAAGAACCCA GAGATCGTGT TTGTGGCGGA TCTGACGCGT 
GCTGAAGCTC CGGCTCTGGT CATGACCACT CAGTGTGAGC TGGTGATGAA GAGCGAGCCC 
AGCGGTCAGT GCATGACGGC CGCCATTTCA GACCTGAAGA TTGTGGCCTG TCCGTTCCTG 
CGGGAGAAGA GGGAGAACAA CGTGACGACG GTCCTACAGC CCTGCGAGGT TTTCTTCCAG 
AGCCGACAGT CAAACACTGA GCCGCAGTGC ATCGAGCTGT CCATCAGCGC ACTCACGCTC 
AAGGTGTCTC CGATCATCAT AAACACGGTC ATCACCATTC AGACGGCTCT AAGTCCTGCC 
GCTGAGACGC CGCAGGAGCT GGAGAGCCCC ATCCCACAGG ACCTATGGGA GCGCCGCAGC 
CTTCGCGACC TGAAGCTCTG GTTCCTGGAG GAGGAGGGCG AGGAGGTCAG CACGTCCGTC 
ACGCGTCTCA TTCCTCAGGG AGAGTCTCTG AAGATGAGCA TCGCCTCCAT CTGCCTGACG 
CTGGAGGCCG GAGTGGGACA CCGCACCGTC CCCATGCTGC TGGCCAAAGC CTCGTTCCAG 
GGGGACGTCA AGGACTGGAG CACTCTCATC AACCTGCACT GCCACCTCAG CATGGAGGTG 
AACTACTATA ATGAGATGAT GGGTTTATGG GAGCCGCTGT TGGAGCCGCT GGACGATGAG 
GATAAAGACG GTTTTAGACC CTGGACTCTC GCACTGAAGA TGAAGAAGAA GCCGGTGGTC 
AGTTCTGGGG TGTCTGATGA GGTGGACGGC GCTGTGCCTG ATTATAAAAC GGTCATCAGC 
TTCACTAGTA AAGACCAGCT TAACGTCACC CTGTCCAAGT GTGGTCTGCA CATGCTCAGT 
AATCTGGGAA CGGCTTTTGC AGAGGCCGCT AAACAGACGG CCGAGTGCTT TCAGACAGAT 
CAGACACCAT TCGTGGTGAA GAATCGCCTG GGTTTGGCGG TGTCTGTGCT GCACAGCGAA 
GTGTTTCGAC CCATCGGGAA GGAGTCTAAG GATCGTGTGG TGGAGCTCCC GGATGGAGAG 
AGTCTGAATT TGGACTATAC CCTCACCGCC GAGTCCAGCG ACCAGTTCTC TGCCATGACC 
AGCCTCAGTG CCAAAAACTA CATACAGCCC ACTCCAGTCG GCCACACGAC CGCAAGTGTG 
ATCCCGCTCA TCAAAACGGG CCGGGGCATG TACAGCGTCA TGCACAACGA GTCCCGCGTC 
ACTCGCTTCT TCGTCTGTCA GATTTACACC GTCGAGGGCA GCAAATATGT CAAGATTCGC 
TCTCCCTTCC AGATTATCAA CCACTTTTCA GTGCCGTTCC GTGTTTATGA AGGATCCGTG 
TTTCTGGGCG AGTCTCTGCC GACAGAGGAG TTCTGTGTGC CGCTGGGATC CTACAGAGCT 
GAGCTGAGTC TGCAGCCTGT GCCTGATGGC GGTGAAGAGT ACGAGCACTC CGAGAGCTTC 
ACCTACACTG AGGTTGTCCA GAAACCCGGA AAGTGTCTGG AGAAAAACTG CCATCTGCGT 
GGTGACAGCG CCCAGATTCT GAAGGTCAAC ATGGTTCCTC TTGCGGACAC AGTGACGTCC 
CACAGCAGCG AGGAGGAGTT CGATAAGCCC ATCGTACTGC ATTTGTGGCC CACCGTCCTG 
CTGCGCAACC TGCTGCCCTA CCCAATTACT TTCAAGCTAA AGGGCATCGG AGATTCCCCC 
TCGGTGTCGC TGAAGGAGGG CCACTCATCC CAGATCCACA CCGCCATCAT GGACTTCTCC 
GTGTTGGATC TGAGGCTGCT GGGATACTTG CAGCAGGACT GGGAGTCTCA GTACAGCCTC 
AGCAGTGAAC AAGACGAGAT CAGCTTCATT AAGTTTCGCT CGCTCGCACG TGAGGAGGAG 
ACTGAGAGCC GGCCGGAGCT GGACATCGCT ATGCATGTGA AGAAGGAGGA GGGTCAGGTT 
GTGCTGGCCG TACACTCGCC GTACTGGATG GTGAATAAAA CAAGCCGACT GCTGCAGTAC 
AAGGCGGATG ATATTCATCG CAAACACCCC AAGGATTACG ACATGCCGCT GCTCTTCTCC 
TTCAAGCCAC GCAACTTCCT GCAAAACAAC AAGATCCGCC TGATGATCTC AGAAAGTGAG 
CTGTCAGATG ATTTCTCCAT CGACACTGTC GGGAGTTACG GAGATGTCAA ATGCAAAGGC 
AGACAGAAGG ATTACTTGGT GGGAGTGAAG ATCGATGCTA GCAGCTTCAG CTTGACACGT 
TTGGTGACTT TTCTGCCATT CTACATGCTG GTGAACAGAA CCAAACATGT GATTCGGGTC 
TGTGAGGAGG GGCAGGAGAA CTGGACAGAA GCCCGACCCG GACAGGCTGC TGTTCCATTC 
TGGCCAGAAA GAGACTCAAA GAAGCTGAGA GTGAAGGTGG AGGGCAGTCA GTCGTCACCA 
CGCGCCTTTG ACTTCCACCA GCCAGAGAAC TGCCTCCTGC TGCATCTGGA CAAAAGCTTG 
GGTGGCATCA TTGTGGATGT GAATCTGACA GAGCATTCAG CTGTGATCCT CTTCTCTGAT 
TATCATGATG GAGCCGCTCC CTTCCTCCTC ATCAACCACA CTAAGGACGA GACGCTGCAG 
TACCACCAGA GCAACAGTAA GAGGAAGCCT TCGACGTGGT TTCAGGATGA AATACTGGAT 
TTATCAATGA GGGATGAGAC TGATTCCCTC TGCTCTCAGG CCGAGATCGA TGAACTGAGT 
CCAGGTCAGG CGGTTTACTA CACCTGGGCT GAACCAACCG GCTCCAGGAT CCTCAGCTGG 
AAACTGGGCA AATACAAAGG AGAGCTCAAG AGTGAAGAGG ATCAGTTGCT GGATGTGGAC 
AAGGAGGGCA AGTTGTTTTT GGTGTCGTTT TATGAAGGCC TCCAGCGTGT GGCGCTCTTC 
ACCAGCGAGC AGCGCATCTT CAAGAAGATC TGCGACAGTG AGAAGATAGA GCTGGCTCAG 
CAAGAAATCA GCATCTTGCT GCAGAACATG GGCATTTCGC TGGTCAACAA CAACTCCAGC 
CAGGAAGTGG CCTTCATTGG CATCACCAGC TCTGATGTTG TGTGGGAAAC CAAGCCGAAG 
AAGCGCAGCC GCTGGAAAGT TCTGAGTGTT AAAGAGGCAG CAGTTCTGGA GCAGCACTAC 
AGAGACTACA TGGAGAACGG CCCAGTGGAC AGCACCATCA TAGACCTGGA CAACGACTAC 
CAGGTGTTGT TTGCTGCTAA CGGTGTGGAC ATGAGGATCA TGCAGCCCTA CGATGCTCCA 
CTCAGAAGGA ACTTCCTGCC GGCGTTGAAG GTGGAGTACA GCGTTTCAGC CCGCCAGAAA 
ACATACAGAG TCCAGATCAA CAGGATCCAG ATCCAAAACC AGCTGCAAGG AGCCATATTC 
CCGTTTGTGT TTTACCCCAT CAAACCGCCA AAGTCTGTCA GCATGGACTC AGAGCCAAAA 
CCGCTGGCTG ACGTGAGCAT CATCACCAGA GCAGCAGGAC ACTCCGACAT TCTGCGCATC 
AAATATTTTA TGGTGTTGAT TCAGGAGATG GACCTGCGGC TGGATCTGGG CTTTCTGTAT 
GCCATACTTG AGCTCTTCAC TGCTGAACAC ACCAGTGCTG TGACGTCTGA ACAGGAGGTG 
GAGTTGTTTG ATAAAGACAT AGAGTATCTA AAGACTGAGT TAAATCATGT TTCAGCTACA 
GACACATCTC CCATCAGCCT CTACGAGTAC TTCCACATCT CTCCTATAAA GCTTCACTTG 
AGCTTGTCTC TGAGCAGTGG TGGTGAAGAC AGCAATAAGC AAGAGCGCGA GACGGAGCTG 
ATCCCAGTTC AGTCTCTCCA TCTGCTGCTC AAGAGCATCG GGGCTACACT TACAGACGCA 
CAGGACGTCC TCTTTAAACT GGGCTTCTTC GAGCTGAGGT ATCAGTTTCG CACCACTCAG 
CAGCTGCAGT GGGAGGTCGT TCGCCATTAC TCCAAACAGG CTATCAAGCA GATGTATGTC 
CTTGTTCTGG GTTTGGACGT TCTTGGGAAT CCATTCGGGC TGATCCGGGG CCTGTCTGAA 
GGCGTCGAGG CTTTCTTTTA CGAGCCCTAT CAGGGTGCAA TTCAGGGTCC AGAGGAGTTC 
ATGGAGGGCA TGGCTCTCGG CGTGAAGGCT CTGGTTGGAG GCGCTGTGGG TGGTCTGGCC 
GGAGCGGCGT CTCGCATCAC TGGAGCGATG GCTAAAGGTG TGGCGGCGAT GACGATGGAT 
GAAGAGTACC AGCAGAAGAG ACGAGAGGCC ATGAACAAAC AGCCCAGCGG ACTGAGAGAA 
GGACTGACAC GAGGAGGAAA AGGACTGGTG TCTGGCTTCG TGAGTGGCAT CACGGGCATT 
GTGACCAAAC CCATTAAAGG AGCTCAGAAG GAAGGAGCGG CAGGTTTCTT CAAGGGTATG 
GGTAAGGGTT TAGTTGGAGC TCTGACCAGA CCAACAGGGG GCATCATTGA CATGGCCAGC 
AGCACCTTCC AGGGCATCAA GAGAGCTGCA GAGCTGTCTC AGGATGTGGA GAGTCTGCGC 
CCTCCACGCT TCATCCACGA GGACGGAGTG ATCCGGCCGT ACAGAGAGAG AGAAGGCCTC 
GGCAGTCAGA TGCTGCAGAG CAGAACTATC GTCTCTCAGT GGAAAACTAC TGATGAGGAC 
TCTGATGAGG ACCAGGAAAA TCAGCTTAAT GACTTCTATT TTCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 03)
Bayés À,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

A protein disulfide isomerase expressed in the embryonic midline is required for left/right asymmetries.
Genes & development16(19)2518-29(2002 Oct)
Hoshijima K,Metherall JE,Grunwald DJ

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VPS13A cDNA ORF clone, Danio rerio(Zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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