THOC2 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	THOC2
Entrez Gene ID	368417
Full Name	THO complex 2
Synonyms	THO,Tho2,fb73e08,fd21d11,si:bz1o18.2,wu:fb73e08,wu:fd21d11
General protein information	Preferred Names THO complex 2 Names THO complex subunit 2
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001326547.1	NP_001313476.1	THO complex subunit 2
XM_005157134.4	XP_005157191.1	THO complex subunit 2 isoform X1
XM_005157134.3	XP_005157191.1	THO complex subunit 2 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Thoc2	NP_001028594.1
Drosophila melanogaster (fruit fly)	tho2	NP_722763.1
Arabidopsis thaliana (thale cress)	THO2	NP_173871.6
Xenopus tropicalis (tropical clawed frog)	thoc2	XP_002931869.1
Bos taurus (cattle)	THOC2	NP_001193626.1
Rattus norvegicus (Norway rat)	Thoc2	NP_001178685.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008236	XP_317233.4
Macaca mulatta (Rhesus monkey)	THOC2	XP_001086217.1
Oryza sativa (rice)	Os01g0916800	NP_001045197.2
Homo sapiens (human)	THOC2	NP_001075019.1
Canis lupus familiaris (dog)	THOC2	XP_005641829.1
Danio rerio (zebrafish)	thoc2	NP_001003847.1
Pan troglodytes (chimpanzee)	THOC2	XP_001139291.2
Gallus gallus (chicken)	THOC2	XP_420332.2
Caenorhabditis elegans (roundworm)	thoc-2	NP_498392.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Drug screening in Scn1a zebrafish mutant identifies clemizole as a potential Dravet syndrome treatment.
Baraban SC, Dinday MT, Hortopan GA
Nature communications42410(2013)

Hematopoietic gene expression profile in zebrafish kidney marrow.
Song HD, Sun XJ, Deng M, Zhang GW, Zhou Y, Wu XY, Sheng Y, Chen Y, Ruan Z, Jiang CL, Fan HY, Zon LI, Kanki JP, Liu TX, Look AT, Chen Z
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)

Identification of 315 genes essential for early zebrafish development.
Amsterdam A, Nissen RM, Sun Z, Swindell EC, Farrington S, Hopkins N
Proceedings of the National Academy of Sciences of the United States of America101(35)12792-7(2004 Aug)

Identification of nodal signaling targets by array analysis of induced complex probes.
Dickmeis T, Aanstad P, Clark M, Fischer N, Herwig R, Mourrain P, Blader P, Rosa F, Lehrach H, Strähle U
Developmental dynamics : an official publication of the American Association of Anatomists222(4)571-80(2001 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following THOC2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the THOC2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa53327	NM_001326547.1 Latest version!	Danio rerio THO complex 2 (thoc2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa19359	XM_005157134.4 Latest version!	Danio rerio THO complex 2 (thoc2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
ODa19359	XM_005157134.3	Danio rerio THO complex 2 (thoc2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

THOC2 ( NM_001326547.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001313476.1 Danio rerio THO complex 2 (thoc2), mRNA.

Price & Availability↑

CloneID	ODa53327
Clone ID Related Accession (Same CDS sequence)	NM_001326547.1
Accession Version	NM_001326547.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1542902400000
Organism	Danio rerio(zebrafish)
Product	THO complex subunit 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX470260.10, EH443657.1, BX510993.6, DT063420.1, BX511226.5, CT728579.2 and EB949114.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGGCGGCGC TGATCCTCCC GTTTGACTGG TTAAAAAACT GGGACAAATC CGGCAGAAAT 
GAGTTTTTAC AGTTTTTTCA AGACCTTGCA GGGAAATCAA GTGGTCATGA GTTGGTTAAA 
GATCTGCAGA CAGCCTTTTA CGAGCTTTGT TGGCATGCTA TCCAGGGCAG TCTGAAGGTG 
GATGTGGCTG CTAGTCTCCT GGCTGATGTT ATGGAACTGC GGGATGATAT GCCTTCTATT 
TTAGCTGATG TTCTCTGTAT ATTAGATATT GAGACTGGTT GTTTGGAGGA GAAACACAAA 
CGGGATCTTT TTACCCAGTT GGTTGGAGCC TGTTTGATCT GTATTCCTGA TGGTGTACTA 
AAGGAGAGAT TAGATCCCGA AACACTGGAG TCTCTGGGGC TGATTAAACA AGCTCAACAG 
TTCAATCAGA AGATTGTGAA AATCAAGACC AAACTGTTTT ACAAACAGCA AAAGTTCAAT 
TTGTTGCGTG AGGAGAATGA AGGTTATGCT AAACTGATCA CAGAGCTTGG CCAAGATCTG 
ACCGGGAATC TCACCAGTCA CATCGTCCTT GAGAACATCA AGTCATTAAT AGGATGTTTC 
AATTTGGACC CAAACCGTGT GCTGGACATC ATTTTGGAGG TGTATGAATG TCGCTCTGAC 
CAGGATGAGT TTTTTATTCC TCTAATCAAG TCCTACATGT GCGAGCACCA GACCCTGTGT 
CACATGCTCG GTTTCAAGTT CAAATTCCAC CAGGAGCCTA ATGGAGAAAC ACCATCATCT 
CTGTATCACA TCGCTGCAGC TCTTCTTCAG CATGACCTCA TCGCTCTTGA AGACCTCTAT 
GTTCATCTTC TCCCACTGGA TTCCAGCATT GTGGAAGAGC ACAAGAGAGA TATCCTAGAG 
GCCAAGCAGA TTGCCCGTAA ACTCACGATG GTGGTCCTAC CCTCTGAAAA GACAGAGGAC 
AAGGAGAAAG AAAAGGACAA GGAAGAAGAA AAAAACGAGA AGCCTCCTGA TAACCAGAAG 
TTGGGACTGC TTGAGGCACT ACTGAGGATC AGTGACTGGC AGCACTCTCA GAGCATCATG 
GATCAATTGC CAGCTTTCTA TGCCACCTCC CACAAGGCCA TCGCTGTTGC ACTGTGCCAG 
CTGCTTCACC TGATCATAGA TCCACTCTAC CGCAGGGCTG GAGTACCAAA AGGTGCCAAA 
GGCAGTTTGA TAACACCTCT GCAGAACAAA CATGCACCCC AGCCCGCCGA AAACTTTGAT 
GATCTATCCA GGGATGTCTT CACCATGTTA TGCTACCTGG GACCTCACCT GTCTCATGAT 
CCCATCCTTT TTGCGAAGAT GGTGCGGCTG GGAAAGAGTT TCATGAAAGA GGACTCTCTA 
CTGGGCAGTT TTCTCAGTAT TGCTGATCAG GTGCTGTTAC CATCACTCTC GCTTATGGAG 
TGCAATGCCT GCATGTCTGA GGAACTCTGG GGCTTTTTTA AATTCTTCCC TTACCAGCAC 
AGGTATCGTC TCTATGGGCA ATGGAAAAAT GAGACATACA GCAATCATCC ACTCCTGGTG 
AAGATCAAGG CTCAGACAGT GGATAGATCC AAATACATCA TGAAGCGGCT CACCAAGGAG 
AATGTTAAGC CCTCTGGAAG GCAGATAGGA AAGTTAAGCC ACAGCAACCC CACCATTCTC 
TTCGATTATA TTCTGTCTCA GATCCAGTGG TACGACAACC TCATAACTCC AGTGGTGGAC 
TCTCTCAAAT ACTTAACATC TCTCAACTAT GATGTACTGG CCTATTGCAT TATTGAAGCT 
TTAGCCAACC CAGAGAAAGA GAAGATGAAA CATGATGACA CAACCATTTC ATCATGGTTG 
CAGAGTCTTG CAAGTCTTTG TGGAGCAGTT TTCAGGAAAT ATCCTATTGA GTTAGCAGGT 
CTTCTCCAGT ACGTAACCAA CCAGCTTAAA GCAGGGAAGA GTTTTGACCT GCTGATCCTG 
AAGGAGGTGG TGCAGAAGAT GGCAGGAATT GAGATAACAG ATGAAATGAC TGTGGAGCAG 
TTGGAGGCCA TGACAGGTGG AGAACAACTC AAAGCAGAGG GTGGTTACTT TGGACAGATT 
AGAAACACTA AGAAATCTTC TCAGCGTCTG AAGGATGCTC TTTTGGATCA TGAGCTGGCA 
TTACCTCTGT GTCTACTGAT GGCCCAGCAG AGGAATGGAG TGGTGTTCTC TGAGGGGGGA 
GAAAAGCATC TAAAACTTGT GGGAAAGCTT TATGATCAGT GTCATGACAC TCTTGTGCAG 
TTTGGTGGCT TTTTGGCATC TAACCTCAGT ACAGAAGACT ACATTAAAAG GGTACCCTCA 
GTTGACGTCC TCTGCAACCA GTTACACACA CCACATGATG CTGCGTTTTT CCTGTCTCGC 
CCGATGTATG CCCATCAGAT CCTGTCCAAG TATGATGAAT TGAAAAAGGC AGAGAAGGGG 
AACAGGCAGC AGCAGAAAGT CCATAAGTAC ATCACAGCCT GCGAGCAGGT TATGGCTCCT 
GTTCATGAGG CTGTCGTGTC TCTTCATTTG CCAAAGGTGT GGGATGATCT TCGACCACAA 
TTCTACGCCA CATTCTGGTC CCTCACCATG TATGACCTGG CAGTACCACA CAATGCCTAC 
GACCGAGAAG TCAACAAGCT TAAGATGCAG ATCAAATCCA TTGATGATAA CACTGAGATG 
CCTCCAAATA AAAAGAAAAA GGAGAAGGAA CGCTGTACAG CTCTGCAGGA CAAGCTACAA 
GAAGAGGAGA AGAAACAGTT GGAGCATGTG CAGAGAGTTC TGCATCGTCT CAAACTGGAG 
AAAGACAATT GGTTACTCGC CAAATCCACT AAGAATGAAA CCATCACTAA GTTCCTTCAG 
CTCTGCATCT TCCCCCGTTG TGTCTTCTCA GCCATTGATG CTGTTTACTG CGCTCGCTTT 
GTGGAGCTGG TTCACCAGCA GAAAACGCCC AACTTCTGCA CCCTACTTTG CTATGACAGA 
GTCTTCTCTG ATATCATCTA TACGGTAGCG AGTTGCACAG AGAATGAGTC CCGCCGATAT 
GGTCGTTTTT TGTGTTGCAT GTTAGAGACT GTCACTCGAT GGCACAGTGA CAGAGCCATT 
TATGAGAAGG AATGTGGTAA TTACCCAGGG TTCCTGACTA TTTTCCGAGC CAGTGGTTTC 
GACGGAGGGA ACAAAGCAGA TCAATTGGAC TATGAGAATT TTCGACATGT TGTCCACAAA 
TGGCATTACA AGTTGACTAA AGCATCTGTT CACTGTCTAG AAACGGGTGA GTACACCCAC 
ATTCGCAACA TTCTGATCGT GCTTACCAAG ATCCTACCCT GGTACCCCAA GGTGCTGAAC 
CTAGGCCAGG CTTTGGAGTG TCGCGTTCAC AAGATCTGCC AGGAAGAGAA GGAGAAGAGG 
CCTGATCTCT ATGCATTAGC CATGGGTTAC TCAGGCCAAT TGAAAGGCAG AAAAGTGCAT 
ATGGTGCCTG AAAATGAGTT CCACCACAAG GAGCAACCAG TACGCAGCAC CACGACCGGC 
ACTCTGCAAA ATGGACCTGG GAATATAGGC AAGACTTCAA CCACTACCGC TGCCAGTGCT 
GGAAAAACTG AGGATGGTGT CGTCGAGGAC TCAGAGAAAT CCAAGGACAA ATCACAGGCA 
GCCCAAAAAA CTGCCAGTAA AAACAGCACA ACAGCCAATA AAGTGAGCAG CAGCAATGGA 
AGCAGCACGC CAAACAGCAT GAAAGGGAGC AAAGAGAAGG AGGACAAAGA AAAGACCACA 
AAAGAGAAAA AGGAGAAAAA AGAAAAGAAC CCAGGGAGCA CTCCAGAGGC CAAGACAGGA 
GCGAAAGACA AGCAAAAGGA AGAAAGGTCT AAGGAAGAGA AGCCCATCGA CAGCAAGGAG 
AAGACACCCA AGGCAGACAA GGACAAGAGG AAGGATGACA AGAAGGAAGA GAAGAGCAAA 
ATCGACGGGA AATCCATTGA AAAAGATTGG TCCAAAGAAC GTGATGCGTC CAGTGACAGC 
AAGACAAAGG AAAGCAGCAA GTTGGAGAAG AATGCTGGGG CTGGAAGCCT GAAGTCCCTT 
GTTCCCAGAT CTGAAGGAGT TGAGTCTGAA CGGGAGCAAA AAAGACGAAA GCTCGACACT 
CACTCGTCGC CATCACACTC CTCTGCAGTT AAGCACCATC CTAACCACAC CACAGTCCTG 
TCCAAGATCA AAGAGAAGGA TGATGAGAAG AAAGAGTCTG ATAAATCCAG GGACCGCTCC 
AAAGAGAGGG AAAAGAAAGA AGACCGGAAG GACCGAAAAC GAGACTACTT GAACAGCGAT 
CGCGAGATGA GCCAGGAAGC CAAACGCCGC AAAGATGAAA ATGGAATGAG CTCATCCAAA 
CACAGCAAGA GTGCCAGCCC CTCTGAATCA CCACACTCCA ATGACAAGGA CAATAGTAAA 
CGCTCCAAGT CCACCAGCAA GGACAAGAGT GATCTGGCCA AATTGGAGAA GACCTCTGGA 
GGCAAAAAGG AATCCAGGCA TGACAAAGAG AAATCAGAAA AAAAGAGAGA CAGCACTGGA 
ACTAAAGAGG AGAAAAAACA CCATAAATCA TCAGAAAAGC ACAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001313476.1
CDS	55..4662
Translation	MAALILPFDWLKNWDKSGRNEFLQFFQDLAGKSSGHELVKDLQTAFYELCWHAIQGSLKVDVAASLLADVMELRDDMPSILADVLCILDIETGCLEEKHKRDLFTQLVGACLICIPDGVLKERLDPETLESLGLIKQAQQFNQKIVKIKTKLFYKQQKFNLLREENEGYAKLITELGQDLTGNLTSHIVLENIKSLIGCFNLDPNRVLDIILEVYECRSDQDEFFIPLIKSYMCEHQTLCHMLGFKFKFHQEPNGETPSSLYHIAAALLQHDLIALEDLYVHLLPLDSSIVEEHKRDILEAKQIARKLTMVVLPSEKTEDKEKEKDKEEEKNEKPPDNQKLGLLEALLRISDWQHSQSIMDQLPAFYATSHKAIAVALCQLLHLIIDPLYRRAGVPKGAKGSLITPLQNKHAPQPAENFDDLSRDVFTMLCYLGPHLSHDPILFAKMVRLGKSFMKEDSLLGSFLSIADQVLLPSLSLMECNACMSEELWGFFKFFPYQHRYRLYGQWKNETYSNHPLLVKIKAQTVDRSKYIMKRLTKENVKPSGRQIGKLSHSNPTILFDYILSQIQWYDNLITPVVDSLKYLTSLNYDVLAYCIIEALANPEKEKMKHDDTTISSWLQSLASLCGAVFRKYPIELAGLLQYVTNQLKAGKSFDLLILKEVVQKMAGIEITDEMTVEQLEAMTGGEQLKAEGGYFGQIRNTKKSSQRLKDALLDHELALPLCLLMAQQRNGVVFSEGGEKHLKLVGKLYDQCHDTLVQFGGFLASNLSTEDYIKRVPSVDVLCNQLHTPHDAAFFLSRPMYAHQILSKYDELKKAEKGNRQQQKVHKYITACEQVMAPVHEAVVSLHLPKVWDDLRPQFYATFWSLTMYDLAVPHNAYDREVNKLKMQIKSIDDNTEMPPNKKKKEKERCTALQDKLQEEEKKQLEHVQRVLHRLKLEKDNWLLAKSTKNETITKFLQLCIFPRCVFSAIDAVYCARFVELVHQQKTPNFCTLLCYDRVFSDIIYTVASCTENESRRYGRFLCCMLETVTRWHSDRAIYEKECGNYPGFLTIFRASGFDGGNKADQLDYENFRHVVHKWHYKLTKASVHCLETGEYTHIRNILIVLTKILPWYPKVLNLGQALECRVHKICQEEKEKRPDLYALAMGYSGQLKGRKVHMVPENEFHHKEQPVRSTTTGTLQNGPGNIGKTSTTTAASAGKTEDGVVEDSEKSKDKSQAAQKTASKNSTTANKVSSSNGSSTPNSMKGSKEKEDKEKTTKEKKEKKEKNPGSTPEAKTGAKDKQKEERSKEEKPIDSKEKTPKADKDKRKDDKKEEKSKIDGKSIEKDWSKERDASSDSKTKESSKLEKNAGAGSLKSLVPRSEGVESEREQKRRKLDTHSSPSHSSAVKHHPNHTTVLSKIKEKDDEKKESDKSRDRSKEREKKEDRKDRKRDYLNSDREMSQEAKRRKDENGMSSSKHSKSASPSESPHSNDKDNSKRSKSTSKDKSDLAKLEKTSGGKKESRHDKEKSEKKRDSTGTKEEKKHHKSSEKHR

Target ORF information:

RefSeq Version	NM_001326547.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio THO complex 2 (thoc2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001326547.1

ORF Insert Sequence:

ATGGCGGCGC TGATCCTCCC GTTTGACTGG TTAAAAAACT GGGACAAATC CGGCAGAAAT 
GAGTTTTTAC AGTTTTTTCA AGACCTTGCA GGGAAATCAA GTGGTCATGA GTTGGTTAAA 
GATCTGCAGA CAGCCTTTTA CGAGCTTTGT TGGCATGCTA TCCAGGGCAG TCTGAAGGTG 
GATGTGGCTG CTAGTCTCCT GGCTGATGTT ATGGAACTGC GGGATGATAT GCCTTCTATT 
TTAGCTGATG TTCTCTGTAT ATTAGATATT GAGACTGGTT GTTTGGAGGA GAAACACAAA 
CGGGATCTTT TTACCCAGTT GGTTGGAGCC TGTTTGATCT GTATTCCTGA TGGTGTACTA 
AAGGAGAGAT TAGATCCCGA AACACTGGAG TCTCTGGGGC TGATTAAACA AGCTCAACAG 
TTCAATCAGA AGATTGTGAA AATCAAGACC AAACTGTTTT ACAAACAGCA AAAGTTCAAT 
TTGTTGCGTG AGGAGAATGA AGGTTATGCT AAACTGATCA CAGAGCTTGG CCAAGATCTG 
ACCGGGAATC TCACCAGTCA CATCGTCCTT GAGAACATCA AGTCATTAAT AGGATGTTTC 
AATTTGGACC CAAACCGTGT GCTGGACATC ATTTTGGAGG TGTATGAATG TCGCTCTGAC 
CAGGATGAGT TTTTTATTCC TCTAATCAAG TCCTACATGT GCGAGCACCA GACCCTGTGT 
CACATGCTCG GTTTCAAGTT CAAATTCCAC CAGGAGCCTA ATGGAGAAAC ACCATCATCT 
CTGTATCACA TCGCTGCAGC TCTTCTTCAG CATGACCTCA TCGCTCTTGA AGACCTCTAT 
GTTCATCTTC TCCCACTGGA TTCCAGCATT GTGGAAGAGC ACAAGAGAGA TATCCTAGAG 
GCCAAGCAGA TTGCCCGTAA ACTCACGATG GTGGTCCTAC CCTCTGAAAA GACAGAGGAC 
AAGGAGAAAG AAAAGGACAA GGAAGAAGAA AAAAACGAGA AGCCTCCTGA TAACCAGAAG 
TTGGGACTGC TTGAGGCACT ACTGAGGATC AGTGACTGGC AGCACTCTCA GAGCATCATG 
GATCAATTGC CAGCTTTCTA TGCCACCTCC CACAAGGCCA TCGCTGTTGC ACTGTGCCAG 
CTGCTTCACC TGATCATAGA TCCACTCTAC CGCAGGGCTG GAGTACCAAA AGGTGCCAAA 
GGCAGTTTGA TAACACCTCT GCAGAACAAA CATGCACCCC AGCCCGCCGA AAACTTTGAT 
GATCTATCCA GGGATGTCTT CACCATGTTA TGCTACCTGG GACCTCACCT GTCTCATGAT 
CCCATCCTTT TTGCGAAGAT GGTGCGGCTG GGAAAGAGTT TCATGAAAGA GGACTCTCTA 
CTGGGCAGTT TTCTCAGTAT TGCTGATCAG GTGCTGTTAC CATCACTCTC GCTTATGGAG 
TGCAATGCCT GCATGTCTGA GGAACTCTGG GGCTTTTTTA AATTCTTCCC TTACCAGCAC 
AGGTATCGTC TCTATGGGCA ATGGAAAAAT GAGACATACA GCAATCATCC ACTCCTGGTG 
AAGATCAAGG CTCAGACAGT GGATAGATCC AAATACATCA TGAAGCGGCT CACCAAGGAG 
AATGTTAAGC CCTCTGGAAG GCAGATAGGA AAGTTAAGCC ACAGCAACCC CACCATTCTC 
TTCGATTATA TTCTGTCTCA GATCCAGTGG TACGACAACC TCATAACTCC AGTGGTGGAC 
TCTCTCAAAT ACTTAACATC TCTCAACTAT GATGTACTGG CCTATTGCAT TATTGAAGCT 
TTAGCCAACC CAGAGAAAGA GAAGATGAAA CATGATGACA CAACCATTTC ATCATGGTTG 
CAGAGTCTTG CAAGTCTTTG TGGAGCAGTT TTCAGGAAAT ATCCTATTGA GTTAGCAGGT 
CTTCTCCAGT ACGTAACCAA CCAGCTTAAA GCAGGGAAGA GTTTTGACCT GCTGATCCTG 
AAGGAGGTGG TGCAGAAGAT GGCAGGAATT GAGATAACAG ATGAAATGAC TGTGGAGCAG 
TTGGAGGCCA TGACAGGTGG AGAACAACTC AAAGCAGAGG GTGGTTACTT TGGACAGATT 
AGAAACACTA AGAAATCTTC TCAGCGTCTG AAGGATGCTC TTTTGGATCA TGAGCTGGCA 
TTACCTCTGT GTCTACTGAT GGCCCAGCAG AGGAATGGAG TGGTGTTCTC TGAGGGGGGA 
GAAAAGCATC TAAAACTTGT GGGAAAGCTT TATGATCAGT GTCATGACAC TCTTGTGCAG 
TTTGGTGGCT TTTTGGCATC TAACCTCAGT ACAGAAGACT ACATTAAAAG GGTACCCTCA 
GTTGACGTCC TCTGCAACCA GTTACACACA CCACATGATG CTGCGTTTTT CCTGTCTCGC 
CCGATGTATG CCCATCAGAT CCTGTCCAAG TATGATGAAT TGAAAAAGGC AGAGAAGGGG 
AACAGGCAGC AGCAGAAAGT CCATAAGTAC ATCACAGCCT GCGAGCAGGT TATGGCTCCT 
GTTCATGAGG CTGTCGTGTC TCTTCATTTG CCAAAGGTGT GGGATGATCT TCGACCACAA 
TTCTACGCCA CATTCTGGTC CCTCACCATG TATGACCTGG CAGTACCACA CAATGCCTAC 
GACCGAGAAG TCAACAAGCT TAAGATGCAG ATCAAATCCA TTGATGATAA CACTGAGATG 
CCTCCAAATA AAAAGAAAAA GGAGAAGGAA CGCTGTACAG CTCTGCAGGA CAAGCTACAA 
GAAGAGGAGA AGAAACAGTT GGAGCATGTG CAGAGAGTTC TGCATCGTCT CAAACTGGAG 
AAAGACAATT GGTTACTCGC CAAATCCACT AAGAATGAAA CCATCACTAA GTTCCTTCAG 
CTCTGCATCT TCCCCCGTTG TGTCTTCTCA GCCATTGATG CTGTTTACTG CGCTCGCTTT 
GTGGAGCTGG TTCACCAGCA GAAAACGCCC AACTTCTGCA CCCTACTTTG CTATGACAGA 
GTCTTCTCTG ATATCATCTA TACGGTAGCG AGTTGCACAG AGAATGAGTC CCGCCGATAT 
GGTCGTTTTT TGTGTTGCAT GTTAGAGACT GTCACTCGAT GGCACAGTGA CAGAGCCATT 
TATGAGAAGG AATGTGGTAA TTACCCAGGG TTCCTGACTA TTTTCCGAGC CAGTGGTTTC 
GACGGAGGGA ACAAAGCAGA TCAATTGGAC TATGAGAATT TTCGACATGT TGTCCACAAA 
TGGCATTACA AGTTGACTAA AGCATCTGTT CACTGTCTAG AAACGGGTGA GTACACCCAC 
ATTCGCAACA TTCTGATCGT GCTTACCAAG ATCCTACCCT GGTACCCCAA GGTGCTGAAC 
CTAGGCCAGG CTTTGGAGTG TCGCGTTCAC AAGATCTGCC AGGAAGAGAA GGAGAAGAGG 
CCTGATCTCT ATGCATTAGC CATGGGTTAC TCAGGCCAAT TGAAAGGCAG AAAAGTGCAT 
ATGGTGCCTG AAAATGAGTT CCACCACAAG GAGCAACCAG TACGCAGCAC CACGACCGGC 
ACTCTGCAAA ATGGACCTGG GAATATAGGC AAGACTTCAA CCACTACCGC TGCCAGTGCT 
GGAAAAACTG AGGATGGTGT CGTCGAGGAC TCAGAGAAAT CCAAGGACAA ATCACAGGCA 
GCCCAAAAAA CTGCCAGTAA AAACAGCACA ACAGCCAATA AAGTGAGCAG CAGCAATGGA 
AGCAGCACGC CAAACAGCAT GAAAGGGAGC AAAGAGAAGG AGGACAAAGA AAAGACCACA 
AAAGAGAAAA AGGAGAAAAA AGAAAAGAAC CCAGGGAGCA CTCCAGAGGC CAAGACAGGA 
GCGAAAGACA AGCAAAAGGA AGAAAGGTCT AAGGAAGAGA AGCCCATCGA CAGCAAGGAG 
AAGACACCCA AGGCAGACAA GGACAAGAGG AAGGATGACA AGAAGGAAGA GAAGAGCAAA 
ATCGACGGGA AATCCATTGA AAAAGATTGG TCCAAAGAAC GTGATGCGTC CAGTGACAGC 
AAGACAAAGG AAAGCAGCAA GTTGGAGAAG AATGCTGGGG CTGGAAGCCT GAAGTCCCTT 
GTTCCCAGAT CTGAAGGAGT TGAGTCTGAA CGGGAGCAAA AAAGACGAAA GCTCGACACT 
CACTCGTCGC CATCACACTC CTCTGCAGTT AAGCACCATC CTAACCACAC CACAGTCCTG 
TCCAAGATCA AAGAGAAGGA TGATGAGAAG AAAGAGTCTG ATAAATCCAG GGACCGCTCC 
AAAGAGAGGG AAAAGAAAGA AGACCGGAAG GACCGAAAAC GAGACTACTT GAACAGCGAT 
CGCGAGATGA GCCAGGAAGC CAAACGCCGC AAAGATGAAA ATGGAATGAG CTCATCCAAA 
CACAGCAAGA GTGCCAGCCC CTCTGAATCA CCACACTCCA ATGACAAGGA CAATAGTAAA 
CGCTCCAAGT CCACCAGCAA GGACAAGAGT GATCTGGCCA AATTGGAGAA GACCTCTGGA 
GGCAAAAAGG AATCCAGGCA TGACAAAGAG AAATCAGAAA AAAAGAGAGA CAGCACTGGA 
ACTAAAGAGG AGAAAAAACA CCATAAATCA TCAGAAAAGC ACAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THOC2 ( XM_005157134.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005157191.1 Danio rerio THO complex 2 (thoc2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa19359
Clone ID Related Accession (Same CDS sequence)	XM_005157134.4 , XM_005157134.3
Accession Version	XM_005157134.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	THO complex subunit 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005157134.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC TGATCCTCCC GTTTGACTGG TTAAAAAACT GGGACAAATC CGGCAGAAAT 
GAGTTTTTAC AGTTTTTTCA AGACCTTGCA GGGAAATCAA GTGGTCATGA GTTGGTTAAA 
GATCTGCAGA CAGCCTTTTA CGAGCTTTGT TGGCATGCTA TCCAGGGCAG TCTGAAGGTG 
GATGTGGCTG CTAGTCTCCT GGCTGATGTT ATGGAACTGC GGGATGATAT GCCTTCTATT 
TTAGCTGATG TTCTCTGTAT ATTAGATATT GAGACTGGTT GTTTGGAGGA GAAACACAAA 
CGGGATCTTT TTACCCAGTT GGTTGGAGCC TGTTTGATCT GTATTCCTGA TGGTGTACTA 
AAGGAGAGAT TAGATCCCGA AACACTGGAG TCTCTGGGGC TGATTAAACA AGCTCAACAG 
TTCAATCAGA AGATTGTGAA AATCAAGACC AAACTGTTTT ACAAACAGCA AAAGTTCAAT 
TTGTTGCGTG AGGAGAATGA AGGTTATGCT AAACTGATCA CAGAGCTTGG CCAAGATCTG 
ACCGGGAATC TCACCAGTCA CATCGTCCTT GAGAACATCA AGTCATTAAT AGGATGTTTC 
AATTTGGACC CAAACCGTGT GCTGGACATC ATTTTGGAGG TGTATGAATG TCGCTCTGAC 
CAGGATGAGT TTTTTATTCC TCTAATCAAG TCCTACATGT GCGAGCACCA GACCCTGTGT 
CACATGCTCG GTTTCAAGTT CAAATTCCAC CAGGAGCCTA ATGGAGAAAC ACCATCATCT 
CTGTATCACA TCGCTGCAGC TCTTCTTCAG CATGACCTCA TCGCTCTTGA AGACCTCTAT 
GTTCATCTTC TCCCACTGGA TTCCAGCATT GTGGAAGAGC ACAAGAGAGA TATCCTAGAG 
GCCAAGCAGA TTGCCCGTAA ACTCACGATG GTGGTCCTAC CCTCTGAAAA GACAGAGGAC 
AAGGAGAAAG AAAAGGACAA GGAAGAAGAA AAAAACGAGA AGCCTCCTGA TAACCAGAAG 
TTGGGACTGC TTGAGGCACT ACTGAGGATC AGTGACTGGC AGCACTCTCA GAGCATCATG 
GATCAATTGC CAGCTTTCTA TGCCACCTCC CACAAGGCCA TCGCTGTTGC ACTGTGCCAG 
CTGCTTCACC TGATCATAGA TCCACTCTAC CGCAGGGCTG GAGTACCAAA AGGTGCCAAA 
GGCAGTTTGA TAACACCTCT GCAGAACAAA CATGCACCCC AGCCCGCCGA AAACTTTGAT 
GATCTATCCA GGGATGTCTT CACCATGTTA TGCTACCTGG GACCTCACCT GTCTCATGAT 
CCCATCCTTT TTGCGAAGAT GGTGCGGCTG GGAAAGAGTT TCATGAAAGA GTACCAAAGT 
GAAAGCAGGC AAGACAGCAG GGACAAAATG GACTCTCTAC TGGGCAGTTT TCTCAGTATT 
GCTGATCAGG TGCTGTTACC ATCACTCTCG CTTATGGAGT GCAATGCCTG CATGTCTGAG 
GAACTCTGGG GCTTTTTTAA ATTCTTCCCT TACCAGCACA GGTATCGTCT CTATGGGCAA 
TGGAAAAATG AGACATACAG CAATCATCCA CTCCTGGTGA AGATCAAGGC TCAGACAGTG 
GATAGATCCA AATACATCAT GAAGCGGCTC ACCAAGGAGA ATGTTAAGCC CTCTGGAAGG 
CAGATAGGAA AGTTAAGCCA CAGCAACCCC ACCATTCTCT TCGATTATAT TCTGTCTCAG 
ATCCAGTGGT ACGACAACCT CATAACTCCA GTGGTGGACT CTCTCAAATA CTTAACATCT 
CTCAACTATG ATGTACTGGC CTATTGCATT ATTGAAGCTT TAGCCAACCC AGAGAAAGAG 
AAGATGAAAC ATGATGACAC AACCATTTCA TCATGGTTGC AGAGTCTTGC AAGTCTTTGT 
GGAGCAGTTT TCAGGAAATA TCCTATTGAG TTAGCAGGTC TTCTCCAGTA CGTAACCAAC 
CAGCTTAAAG CAGGGAAGAG TTTTGACCTG CTGATCCTGA AGGAGGTGGT GCAGAAGATG 
GCAGGAATTG AGATAACAGA TGAAATGACT GTGGAGCAGT TGGAGGCCAT GACAGGTGGA 
GAACAACTCA AAGCAGAGGG TGGTTACTTT GGACAGATTA GAAACACTAA GAAATCTTCT 
CAGCGTCTGA AGGATGCTCT TTTGGATCAT GAGCTGGCAT TACCTCTGTG TCTACTGATG 
GCCCAGCAGA GGAATGGAGT GGTGTTCTCT GAGGGGGGAG AAAAGCATCT AAAACTTGTG 
GGAAAGCTTT ATGATCAGTG TCATGACACT CTTGTGCAGT TTGGTGGCTT TTTGGCATCT 
AACCTCAGTA CAGAAGACTA CATTAAAAGG GTACCCTCAG TTGACGTCCT CTGCAACCAG 
TTACACACAC CACATGATGC TGCGTTTTTC CTGTCTCGCC CGATGTATGC CCATCAGATC 
CTGTCCAAGT ATGATGAATT GAAAAAGGCA GAGAAGGGGA ACAGGCAGCA GCAGAAAGTC 
CATAAGTACA TCACAGCCTG CGAGCAGGTT ATGGCTCCTG TTCATGAGGC TGTCGTGTCT 
CTTCATTTGC CAAAGGTGTG GGATGATCTT CGACCACAAT TCTACGCCAC ATTCTGGTCC 
CTCACCATGT ATGACCTGGC AGTACCACAC AATGCCTACG ACCGAGAAGT CAACAAGCTT 
AAGATGCAGA TCAAATCCAT TGATGATAAC ACTGAGATGC CTCCAAATAA AAAGAAAAAG 
GAGAAGGAAC GCTGTACAGC TCTGCAGGAC AAGCTACAAG AAGAGGAGAA GAAACAGTTG 
GAGCATGTGC AGAGAGTTCT GCATCGTCTC AAACTGGAGA AAGACAATTG GTTACTCGCC 
AAATCCACTA AGAATGAAAC CATCACTAAG TTCCTTCAGC TCTGCATCTT CCCCCGTTGT 
GTCTTCTCAG CCATTGATGC TGTTTACTGC GCTCGCTTTG TGGAGCTGGT TCACCAGCAG 
AAAACGCCCA ACTTCTGCAC CCTACTTTGC TATGACAGAG TCTTCTCTGA TATCATCTAT 
ACGGTAGCGA GTTGCACAGA GAATGAGTCC CGCCGATATG GTCGTTTTTT GTGTTGCATG 
TTAGAGACTG TCACTCGATG GCACAGTGAC AGAGCCATTT ATGAGAAGGA ATGTGGTAAT 
TACCCAGGGT TCCTGACTAT TTTCCGAGCC AGTGGTTTCG ACGGAGGGAA CAAAGCAGAT 
CAATTGGACT ATGAGAATTT TCGACATGTT GTCCACAAAT GGCATTACAA GTTGACTAAA 
GCATCTGTTC ACTGTCTAGA AACGGGTGAG TACACCCACA TTCGCAACAT TCTGATCGTG 
CTTACCAAGA TCCTACCCTG GTACCCCAAG GTGCTGAACC TAGGCCAGGC TTTGGAGTGT 
CGCGTTCACA AGATCTGCCA GGAAGAGAAG GAGAAGAGGC CTGATCTCTA TGCATTAGCC 
ATGGGTTACT CAGGCCAATT GAAAGGCAGA AAAGTGCATA TGGTGCCTGA AAATGAGTTC 
CACCACAAGG AGCAACCAGT ACGCAGCACC ACGACCGGCA CTCTGCAAAA TGGACCTGGG 
AATATAGGCA AGACTTCAAC CACTACCGCT GCCAGTGCTG GAAAAACTGA GGATGGTGTC 
GTCGAGGACT CAGAGAAATC CAAGGACAAA TCACAGGCAG CCCAAAAAAC TGCCAGTAAA 
AACAGCACAA CAGCCAATAA AGTGAGCAGC AGCAATGGAA GCAGCACGCC AAACAGCATG 
AAAGGGAGCA AAGAGAAGGA GGACAAAGAA AAGACCACAA AAGAGAAAAA GGAGAAAAAA 
GAAAAGAACC CAGGGAGCAC TCCAGAGGCC AAGACAGGAG CGAAAGACAA GCAAAAGGAA 
GAAAGGTCTA AGGAAGAGAA GCCCATCGAC AGCAAGGAGA AGACACCCAA GGCAGACAAG 
GACAAGAGGA AGGATGACAA GAAGGAAGAG AAGAGCAAAA TCGACGGGAA ATCCATTGAA 
AAAGATTGGT CCAAAGAACG TGATGCGTCC AGTGACAGCA AGACAAAGGA AAGCAGCAAG 
TTGGAGAAGA ATGCTGGGGC TGGAAGCCTG AAGTCCCTTG TTCCCAGATC TGAAGGAGTT 
GAGTCTGAAC GGGAGCAAAA AAGACGAAAG CTCGACACTC ACTCGTCGCC ATCACACTCC 
TCTGCAGTTA AGGACAATCT CAACGACTTC AAGGACTCCA CATTAAAGCA CCATCCTAAC 
CACACCACAG TCCTGTCCAA GATCAAAGAG AAGGATGATG AGAAGAAAGA GTCTGATAAA 
TCCAGGGACC GCTCCAAAGA GAGGGAAAAG AAAGAAGACC GGAAGGACCG AAAACGAGAC 
TACTTGAACA GCGATCGCGA GATGAGCCAG GAAGCCAAAC GCCGCAAAGA TGAAAATGGA 
ATGAGCTCAT CCAAACACAG CAAGAGTGCC AGCCCCTCTG AATCACCACA CTCCAATGAC 
AAGGACAATA GTAAACGCTC CAAGTCCACC AGCAAGGACA AGAGTGATCT GGCCAAATTG 
GAGAAGACCT CTGGAGGCAA AAAGGAATCC AGGCATGACA AAGAGAAATC AGAAAAAAAG 
AGAGACAGCA CTGGAACTAA AGAGGAGAAA AAACACCATA AATCATCAGA AAAGCACAGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157191.1
CDS	56..4738
Translation	MAALILPFDWLKNWDKSGRNEFLQFFQDLAGKSSGHELVKDLQTAFYELCWHAIQGSLKVDVAASLLADVMELRDDMPSILADVLCILDIETGCLEEKHKRDLFTQLVGACLICIPDGVLKERLDPETLESLGLIKQAQQFNQKIVKIKTKLFYKQQKFNLLREENEGYAKLITELGQDLTGNLTSHIVLENIKSLIGCFNLDPNRVLDIILEVYECRSDQDEFFIPLIKSYMCEHQTLCHMLGFKFKFHQEPNGETPSSLYHIAAALLQHDLIALEDLYVHLLPLDSSIVEEHKRDILEAKQIARKLTMVVLPSEKTEDKEKEKDKEEEKNEKPPDNQKLGLLEALLRISDWQHSQSIMDQLPAFYATSHKAIAVALCQLLHLIIDPLYRRAGVPKGAKGSLITPLQNKHAPQPAENFDDLSRDVFTMLCYLGPHLSHDPILFAKMVRLGKSFMKEYQSESRQDSRDKMDSLLGSFLSIADQVLLPSLSLMECNACMSEELWGFFKFFPYQHRYRLYGQWKNETYSNHPLLVKIKAQTVDRSKYIMKRLTKENVKPSGRQIGKLSHSNPTILFDYILSQIQWYDNLITPVVDSLKYLTSLNYDVLAYCIIEALANPEKEKMKHDDTTISSWLQSLASLCGAVFRKYPIELAGLLQYVTNQLKAGKSFDLLILKEVVQKMAGIEITDEMTVEQLEAMTGGEQLKAEGGYFGQIRNTKKSSQRLKDALLDHELALPLCLLMAQQRNGVVFSEGGEKHLKLVGKLYDQCHDTLVQFGGFLASNLSTEDYIKRVPSVDVLCNQLHTPHDAAFFLSRPMYAHQILSKYDELKKAEKGNRQQQKVHKYITACEQVMAPVHEAVVSLHLPKVWDDLRPQFYATFWSLTMYDLAVPHNAYDREVNKLKMQIKSIDDNTEMPPNKKKKEKERCTALQDKLQEEEKKQLEHVQRVLHRLKLEKDNWLLAKSTKNETITKFLQLCIFPRCVFSAIDAVYCARFVELVHQQKTPNFCTLLCYDRVFSDIIYTVASCTENESRRYGRFLCCMLETVTRWHSDRAIYEKECGNYPGFLTIFRASGFDGGNKADQLDYENFRHVVHKWHYKLTKASVHCLETGEYTHIRNILIVLTKILPWYPKVLNLGQALECRVHKICQEEKEKRPDLYALAMGYSGQLKGRKVHMVPENEFHHKEQPVRSTTTGTLQNGPGNIGKTSTTTAASAGKTEDGVVEDSEKSKDKSQAAQKTASKNSTTANKVSSSNGSSTPNSMKGSKEKEDKEKTTKEKKEKKEKNPGSTPEAKTGAKDKQKEERSKEEKPIDSKEKTPKADKDKRKDDKKEEKSKIDGKSIEKDWSKERDASSDSKTKESSKLEKNAGAGSLKSLVPRSEGVESEREQKRRKLDTHSSPSHSSAVKDNLNDFKDSTLKHHPNHTTVLSKIKEKDDEKKESDKSRDRSKEREKKEDRKDRKRDYLNSDREMSQEAKRRKDENGMSSSKHSKSASPSESPHSNDKDNSKRSKSTSKDKSDLAKLEKTSGGKKESRHDKEKSEKKRDSTGTKEEKKHHKSSEKHR

Target ORF information:

RefSeq Version	XM_005157134.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio THO complex 2 (thoc2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157134.4

ORF Insert Sequence:

ATGGCGGCGC TGATCCTCCC GTTTGACTGG TTAAAAAACT GGGACAAATC CGGCAGAAAT 
GAGTTTTTAC AGTTTTTTCA AGACCTTGCA GGGAAATCAA GTGGTCATGA GTTGGTTAAA 
GATCTGCAGA CAGCCTTTTA CGAGCTTTGT TGGCATGCTA TCCAGGGCAG TCTGAAGGTG 
GATGTGGCTG CTAGTCTCCT GGCTGATGTT ATGGAACTGC GGGATGATAT GCCTTCTATT 
TTAGCTGATG TTCTCTGTAT ATTAGATATT GAGACTGGTT GTTTGGAGGA GAAACACAAA 
CGGGATCTTT TTACCCAGTT GGTTGGAGCC TGTTTGATCT GTATTCCTGA TGGTGTACTA 
AAGGAGAGAT TAGATCCCGA AACACTGGAG TCTCTGGGGC TGATTAAACA AGCTCAACAG 
TTCAATCAGA AGATTGTGAA AATCAAGACC AAACTGTTTT ACAAACAGCA AAAGTTCAAT 
TTGTTGCGTG AGGAGAATGA AGGTTATGCT AAACTGATCA CAGAGCTTGG CCAAGATCTG 
ACCGGGAATC TCACCAGTCA CATCGTCCTT GAGAACATCA AGTCATTAAT AGGATGTTTC 
AATTTGGACC CAAACCGTGT GCTGGACATC ATTTTGGAGG TGTATGAATG TCGCTCTGAC 
CAGGATGAGT TTTTTATTCC TCTAATCAAG TCCTACATGT GCGAGCACCA GACCCTGTGT 
CACATGCTCG GTTTCAAGTT CAAATTCCAC CAGGAGCCTA ATGGAGAAAC ACCATCATCT 
CTGTATCACA TCGCTGCAGC TCTTCTTCAG CATGACCTCA TCGCTCTTGA AGACCTCTAT 
GTTCATCTTC TCCCACTGGA TTCCAGCATT GTGGAAGAGC ACAAGAGAGA TATCCTAGAG 
GCCAAGCAGA TTGCCCGTAA ACTCACGATG GTGGTCCTAC CCTCTGAAAA GACAGAGGAC 
AAGGAGAAAG AAAAGGACAA GGAAGAAGAA AAAAACGAGA AGCCTCCTGA TAACCAGAAG 
TTGGGACTGC TTGAGGCACT ACTGAGGATC AGTGACTGGC AGCACTCTCA GAGCATCATG 
GATCAATTGC CAGCTTTCTA TGCCACCTCC CACAAGGCCA TCGCTGTTGC ACTGTGCCAG 
CTGCTTCACC TGATCATAGA TCCACTCTAC CGCAGGGCTG GAGTACCAAA AGGTGCCAAA 
GGCAGTTTGA TAACACCTCT GCAGAACAAA CATGCACCCC AGCCCGCCGA AAACTTTGAT 
GATCTATCCA GGGATGTCTT CACCATGTTA TGCTACCTGG GACCTCACCT GTCTCATGAT 
CCCATCCTTT TTGCGAAGAT GGTGCGGCTG GGAAAGAGTT TCATGAAAGA GTACCAAAGT 
GAAAGCAGGC AAGACAGCAG GGACAAAATG GACTCTCTAC TGGGCAGTTT TCTCAGTATT 
GCTGATCAGG TGCTGTTACC ATCACTCTCG CTTATGGAGT GCAATGCCTG CATGTCTGAG 
GAACTCTGGG GCTTTTTTAA ATTCTTCCCT TACCAGCACA GGTATCGTCT CTATGGGCAA 
TGGAAAAATG AGACATACAG CAATCATCCA CTCCTGGTGA AGATCAAGGC TCAGACAGTG 
GATAGATCCA AATACATCAT GAAGCGGCTC ACCAAGGAGA ATGTTAAGCC CTCTGGAAGG 
CAGATAGGAA AGTTAAGCCA CAGCAACCCC ACCATTCTCT TCGATTATAT TCTGTCTCAG 
ATCCAGTGGT ACGACAACCT CATAACTCCA GTGGTGGACT CTCTCAAATA CTTAACATCT 
CTCAACTATG ATGTACTGGC CTATTGCATT ATTGAAGCTT TAGCCAACCC AGAGAAAGAG 
AAGATGAAAC ATGATGACAC AACCATTTCA TCATGGTTGC AGAGTCTTGC AAGTCTTTGT 
GGAGCAGTTT TCAGGAAATA TCCTATTGAG TTAGCAGGTC TTCTCCAGTA CGTAACCAAC 
CAGCTTAAAG CAGGGAAGAG TTTTGACCTG CTGATCCTGA AGGAGGTGGT GCAGAAGATG 
GCAGGAATTG AGATAACAGA TGAAATGACT GTGGAGCAGT TGGAGGCCAT GACAGGTGGA 
GAACAACTCA AAGCAGAGGG TGGTTACTTT GGACAGATTA GAAACACTAA GAAATCTTCT 
CAGCGTCTGA AGGATGCTCT TTTGGATCAT GAGCTGGCAT TACCTCTGTG TCTACTGATG 
GCCCAGCAGA GGAATGGAGT GGTGTTCTCT GAGGGGGGAG AAAAGCATCT AAAACTTGTG 
GGAAAGCTTT ATGATCAGTG TCATGACACT CTTGTGCAGT TTGGTGGCTT TTTGGCATCT 
AACCTCAGTA CAGAAGACTA CATTAAAAGG GTACCCTCAG TTGACGTCCT CTGCAACCAG 
TTACACACAC CACATGATGC TGCGTTTTTC CTGTCTCGCC CGATGTATGC CCATCAGATC 
CTGTCCAAGT ATGATGAATT GAAAAAGGCA GAGAAGGGGA ACAGGCAGCA GCAGAAAGTC 
CATAAGTACA TCACAGCCTG CGAGCAGGTT ATGGCTCCTG TTCATGAGGC TGTCGTGTCT 
CTTCATTTGC CAAAGGTGTG GGATGATCTT CGACCACAAT TCTACGCCAC ATTCTGGTCC 
CTCACCATGT ATGACCTGGC AGTACCACAC AATGCCTACG ACCGAGAAGT CAACAAGCTT 
AAGATGCAGA TCAAATCCAT TGATGATAAC ACTGAGATGC CTCCAAATAA AAAGAAAAAG 
GAGAAGGAAC GCTGTACAGC TCTGCAGGAC AAGCTACAAG AAGAGGAGAA GAAACAGTTG 
GAGCATGTGC AGAGAGTTCT GCATCGTCTC AAACTGGAGA AAGACAATTG GTTACTCGCC 
AAATCCACTA AGAATGAAAC CATCACTAAG TTCCTTCAGC TCTGCATCTT CCCCCGTTGT 
GTCTTCTCAG CCATTGATGC TGTTTACTGC GCTCGCTTTG TGGAGCTGGT TCACCAGCAG 
AAAACGCCCA ACTTCTGCAC CCTACTTTGC TATGACAGAG TCTTCTCTGA TATCATCTAT 
ACGGTAGCGA GTTGCACAGA GAATGAGTCC CGCCGATATG GTCGTTTTTT GTGTTGCATG 
TTAGAGACTG TCACTCGATG GCACAGTGAC AGAGCCATTT ATGAGAAGGA ATGTGGTAAT 
TACCCAGGGT TCCTGACTAT TTTCCGAGCC AGTGGTTTCG ACGGAGGGAA CAAAGCAGAT 
CAATTGGACT ATGAGAATTT TCGACATGTT GTCCACAAAT GGCATTACAA GTTGACTAAA 
GCATCTGTTC ACTGTCTAGA AACGGGTGAG TACACCCACA TTCGCAACAT TCTGATCGTG 
CTTACCAAGA TCCTACCCTG GTACCCCAAG GTGCTGAACC TAGGCCAGGC TTTGGAGTGT 
CGCGTTCACA AGATCTGCCA GGAAGAGAAG GAGAAGAGGC CTGATCTCTA TGCATTAGCC 
ATGGGTTACT CAGGCCAATT GAAAGGCAGA AAAGTGCATA TGGTGCCTGA AAATGAGTTC 
CACCACAAGG AGCAACCAGT ACGCAGCACC ACGACCGGCA CTCTGCAAAA TGGACCTGGG 
AATATAGGCA AGACTTCAAC CACTACCGCT GCCAGTGCTG GAAAAACTGA GGATGGTGTC 
GTCGAGGACT CAGAGAAATC CAAGGACAAA TCACAGGCAG CCCAAAAAAC TGCCAGTAAA 
AACAGCACAA CAGCCAATAA AGTGAGCAGC AGCAATGGAA GCAGCACGCC AAACAGCATG 
AAAGGGAGCA AAGAGAAGGA GGACAAAGAA AAGACCACAA AAGAGAAAAA GGAGAAAAAA 
GAAAAGAACC CAGGGAGCAC TCCAGAGGCC AAGACAGGAG CGAAAGACAA GCAAAAGGAA 
GAAAGGTCTA AGGAAGAGAA GCCCATCGAC AGCAAGGAGA AGACACCCAA GGCAGACAAG 
GACAAGAGGA AGGATGACAA GAAGGAAGAG AAGAGCAAAA TCGACGGGAA ATCCATTGAA 
AAAGATTGGT CCAAAGAACG TGATGCGTCC AGTGACAGCA AGACAAAGGA AAGCAGCAAG 
TTGGAGAAGA ATGCTGGGGC TGGAAGCCTG AAGTCCCTTG TTCCCAGATC TGAAGGAGTT 
GAGTCTGAAC GGGAGCAAAA AAGACGAAAG CTCGACACTC ACTCGTCGCC ATCACACTCC 
TCTGCAGTTA AGGACAATCT CAACGACTTC AAGGACTCCA CATTAAAGCA CCATCCTAAC 
CACACCACAG TCCTGTCCAA GATCAAAGAG AAGGATGATG AGAAGAAAGA GTCTGATAAA 
TCCAGGGACC GCTCCAAAGA GAGGGAAAAG AAAGAAGACC GGAAGGACCG AAAACGAGAC 
TACTTGAACA GCGATCGCGA GATGAGCCAG GAAGCCAAAC GCCGCAAAGA TGAAAATGGA 
ATGAGCTCAT CCAAACACAG CAAGAGTGCC AGCCCCTCTG AATCACCACA CTCCAATGAC 
AAGGACAATA GTAAACGCTC CAAGTCCACC AGCAAGGACA AGAGTGATCT GGCCAAATTG 
GAGAAGACCT CTGGAGGCAA AAAGGAATCC AGGCATGACA AAGAGAAATC AGAAAAAAAG 
AGAGACAGCA CTGGAACTAA AGAGGAGAAA AAACACCATA AATCATCAGA AAAGCACAGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THOC2 ( XM_005157134.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005157191.1 Danio rerio THO complex 2 (thoc2), transcript variant X1, mRNA.

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CloneID	ODa19359
Clone ID Related Accession (Same CDS sequence)	XM_005157134.4 , XM_005157134.3
Accession Version	XM_005157134.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	THO complex subunit 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005157134.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC TGATCCTCCC GTTTGACTGG TTAAAAAACT GGGACAAATC CGGCAGAAAT 
GAGTTTTTAC AGTTTTTTCA AGACCTTGCA GGGAAATCAA GTGGTCATGA GTTGGTTAAA 
GATCTGCAGA CAGCCTTTTA CGAGCTTTGT TGGCATGCTA TCCAGGGCAG TCTGAAGGTG 
GATGTGGCTG CTAGTCTCCT GGCTGATGTT ATGGAACTGC GGGATGATAT GCCTTCTATT 
TTAGCTGATG TTCTCTGTAT ATTAGATATT GAGACTGGTT GTTTGGAGGA GAAACACAAA 
CGGGATCTTT TTACCCAGTT GGTTGGAGCC TGTTTGATCT GTATTCCTGA TGGTGTACTA 
AAGGAGAGAT TAGATCCCGA AACACTGGAG TCTCTGGGGC TGATTAAACA AGCTCAACAG 
TTCAATCAGA AGATTGTGAA AATCAAGACC AAACTGTTTT ACAAACAGCA AAAGTTCAAT 
TTGTTGCGTG AGGAGAATGA AGGTTATGCT AAACTGATCA CAGAGCTTGG CCAAGATCTG 
ACCGGGAATC TCACCAGTCA CATCGTCCTT GAGAACATCA AGTCATTAAT AGGATGTTTC 
AATTTGGACC CAAACCGTGT GCTGGACATC ATTTTGGAGG TGTATGAATG TCGCTCTGAC 
CAGGATGAGT TTTTTATTCC TCTAATCAAG TCCTACATGT GCGAGCACCA GACCCTGTGT 
CACATGCTCG GTTTCAAGTT CAAATTCCAC CAGGAGCCTA ATGGAGAAAC ACCATCATCT 
CTGTATCACA TCGCTGCAGC TCTTCTTCAG CATGACCTCA TCGCTCTTGA AGACCTCTAT 
GTTCATCTTC TCCCACTGGA TTCCAGCATT GTGGAAGAGC ACAAGAGAGA TATCCTAGAG 
GCCAAGCAGA TTGCCCGTAA ACTCACGATG GTGGTCCTAC CCTCTGAAAA GACAGAGGAC 
AAGGAGAAAG AAAAGGACAA GGAAGAAGAA AAAAACGAGA AGCCTCCTGA TAACCAGAAG 
TTGGGACTGC TTGAGGCACT ACTGAGGATC AGTGACTGGC AGCACTCTCA GAGCATCATG 
GATCAATTGC CAGCTTTCTA TGCCACCTCC CACAAGGCCA TCGCTGTTGC ACTGTGCCAG 
CTGCTTCACC TGATCATAGA TCCACTCTAC CGCAGGGCTG GAGTACCAAA AGGTGCCAAA 
GGCAGTTTGA TAACACCTCT GCAGAACAAA CATGCACCCC AGCCCGCCGA AAACTTTGAT 
GATCTATCCA GGGATGTCTT CACCATGTTA TGCTACCTGG GACCTCACCT GTCTCATGAT 
CCCATCCTTT TTGCGAAGAT GGTGCGGCTG GGAAAGAGTT TCATGAAAGA GTACCAAAGT 
GAAAGCAGGC AAGACAGCAG GGACAAAATG GACTCTCTAC TGGGCAGTTT TCTCAGTATT 
GCTGATCAGG TGCTGTTACC ATCACTCTCG CTTATGGAGT GCAATGCCTG CATGTCTGAG 
GAACTCTGGG GCTTTTTTAA ATTCTTCCCT TACCAGCACA GGTATCGTCT CTATGGGCAA 
TGGAAAAATG AGACATACAG CAATCATCCA CTCCTGGTGA AGATCAAGGC TCAGACAGTG 
GATAGATCCA AATACATCAT GAAGCGGCTC ACCAAGGAGA ATGTTAAGCC CTCTGGAAGG 
CAGATAGGAA AGTTAAGCCA CAGCAACCCC ACCATTCTCT TCGATTATAT TCTGTCTCAG 
ATCCAGTGGT ACGACAACCT CATAACTCCA GTGGTGGACT CTCTCAAATA CTTAACATCT 
CTCAACTATG ATGTACTGGC CTATTGCATT ATTGAAGCTT TAGCCAACCC AGAGAAAGAG 
AAGATGAAAC ATGATGACAC AACCATTTCA TCATGGTTGC AGAGTCTTGC AAGTCTTTGT 
GGAGCAGTTT TCAGGAAATA TCCTATTGAG TTAGCAGGTC TTCTCCAGTA CGTAACCAAC 
CAGCTTAAAG CAGGGAAGAG TTTTGACCTG CTGATCCTGA AGGAGGTGGT GCAGAAGATG 
GCAGGAATTG AGATAACAGA TGAAATGACT GTGGAGCAGT TGGAGGCCAT GACAGGTGGA 
GAACAACTCA AAGCAGAGGG TGGTTACTTT GGACAGATTA GAAACACTAA GAAATCTTCT 
CAGCGTCTGA AGGATGCTCT TTTGGATCAT GAGCTGGCAT TACCTCTGTG TCTACTGATG 
GCCCAGCAGA GGAATGGAGT GGTGTTCTCT GAGGGGGGAG AAAAGCATCT AAAACTTGTG 
GGAAAGCTTT ATGATCAGTG TCATGACACT CTTGTGCAGT TTGGTGGCTT TTTGGCATCT 
AACCTCAGTA CAGAAGACTA CATTAAAAGG GTACCCTCAG TTGACGTCCT CTGCAACCAG 
TTACACACAC CACATGATGC TGCGTTTTTC CTGTCTCGCC CGATGTATGC CCATCAGATC 
CTGTCCAAGT ATGATGAATT GAAAAAGGCA GAGAAGGGGA ACAGGCAGCA GCAGAAAGTC 
CATAAGTACA TCACAGCCTG CGAGCAGGTT ATGGCTCCTG TTCATGAGGC TGTCGTGTCT 
CTTCATTTGC CAAAGGTGTG GGATGATCTT CGACCACAAT TCTACGCCAC ATTCTGGTCC 
CTCACCATGT ATGACCTGGC AGTACCACAC AATGCCTACG ACCGAGAAGT CAACAAGCTT 
AAGATGCAGA TCAAATCCAT TGATGATAAC ACTGAGATGC CTCCAAATAA AAAGAAAAAG 
GAGAAGGAAC GCTGTACAGC TCTGCAGGAC AAGCTACAAG AAGAGGAGAA GAAACAGTTG 
GAGCATGTGC AGAGAGTTCT GCATCGTCTC AAACTGGAGA AAGACAATTG GTTACTCGCC 
AAATCCACTA AGAATGAAAC CATCACTAAG TTCCTTCAGC TCTGCATCTT CCCCCGTTGT 
GTCTTCTCAG CCATTGATGC TGTTTACTGC GCTCGCTTTG TGGAGCTGGT TCACCAGCAG 
AAAACGCCCA ACTTCTGCAC CCTACTTTGC TATGACAGAG TCTTCTCTGA TATCATCTAT 
ACGGTAGCGA GTTGCACAGA GAATGAGTCC CGCCGATATG GTCGTTTTTT GTGTTGCATG 
TTAGAGACTG TCACTCGATG GCACAGTGAC AGAGCCATTT ATGAGAAGGA ATGTGGTAAT 
TACCCAGGGT TCCTGACTAT TTTCCGAGCC AGTGGTTTCG ACGGAGGGAA CAAAGCAGAT 
CAATTGGACT ATGAGAATTT TCGACATGTT GTCCACAAAT GGCATTACAA GTTGACTAAA 
GCATCTGTTC ACTGTCTAGA AACGGGTGAG TACACCCACA TTCGCAACAT TCTGATCGTG 
CTTACCAAGA TCCTACCCTG GTACCCCAAG GTGCTGAACC TAGGCCAGGC TTTGGAGTGT 
CGCGTTCACA AGATCTGCCA GGAAGAGAAG GAGAAGAGGC CTGATCTCTA TGCATTAGCC 
ATGGGTTACT CAGGCCAATT GAAAGGCAGA AAAGTGCATA TGGTGCCTGA AAATGAGTTC 
CACCACAAGG AGCAACCAGT ACGCAGCACC ACGACCGGCA CTCTGCAAAA TGGACCTGGG 
AATATAGGCA AGACTTCAAC CACTACCGCT GCCAGTGCTG GAAAAACTGA GGATGGTGTC 
GTCGAGGACT CAGAGAAATC CAAGGACAAA TCACAGGCAG CCCAAAAAAC TGCCAGTAAA 
AACAGCACAA CAGCCAATAA AGTGAGCAGC AGCAATGGAA GCAGCACGCC AAACAGCATG 
AAAGGGAGCA AAGAGAAGGA GGACAAAGAA AAGACCACAA AAGAGAAAAA GGAGAAAAAA 
GAAAAGAACC CAGGGAGCAC TCCAGAGGCC AAGACAGGAG CGAAAGACAA GCAAAAGGAA 
GAAAGGTCTA AGGAAGAGAA GCCCATCGAC AGCAAGGAGA AGACACCCAA GGCAGACAAG 
GACAAGAGGA AGGATGACAA GAAGGAAGAG AAGAGCAAAA TCGACGGGAA ATCCATTGAA 
AAAGATTGGT CCAAAGAACG TGATGCGTCC AGTGACAGCA AGACAAAGGA AAGCAGCAAG 
TTGGAGAAGA ATGCTGGGGC TGGAAGCCTG AAGTCCCTTG TTCCCAGATC TGAAGGAGTT 
GAGTCTGAAC GGGAGCAAAA AAGACGAAAG CTCGACACTC ACTCGTCGCC ATCACACTCC 
TCTGCAGTTA AGGACAATCT CAACGACTTC AAGGACTCCA CATTAAAGCA CCATCCTAAC 
CACACCACAG TCCTGTCCAA GATCAAAGAG AAGGATGATG AGAAGAAAGA GTCTGATAAA 
TCCAGGGACC GCTCCAAAGA GAGGGAAAAG AAAGAAGACC GGAAGGACCG AAAACGAGAC 
TACTTGAACA GCGATCGCGA GATGAGCCAG GAAGCCAAAC GCCGCAAAGA TGAAAATGGA 
ATGAGCTCAT CCAAACACAG CAAGAGTGCC AGCCCCTCTG AATCACCACA CTCCAATGAC 
AAGGACAATA GTAAACGCTC CAAGTCCACC AGCAAGGACA AGAGTGATCT GGCCAAATTG 
GAGAAGACCT CTGGAGGCAA AAAGGAATCC AGGCATGACA AAGAGAAATC AGAAAAAAAG 
AGAGACAGCA CTGGAACTAA AGAGGAGAAA AAACACCATA AATCATCAGA AAAGCACAGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157191.1
CDS	49..4731
Translation	MAALILPFDWLKNWDKSGRNEFLQFFQDLAGKSSGHELVKDLQTAFYELCWHAIQGSLKVDVAASLLADVMELRDDMPSILADVLCILDIETGCLEEKHKRDLFTQLVGACLICIPDGVLKERLDPETLESLGLIKQAQQFNQKIVKIKTKLFYKQQKFNLLREENEGYAKLITELGQDLTGNLTSHIVLENIKSLIGCFNLDPNRVLDIILEVYECRSDQDEFFIPLIKSYMCEHQTLCHMLGFKFKFHQEPNGETPSSLYHIAAALLQHDLIALEDLYVHLLPLDSSIVEEHKRDILEAKQIARKLTMVVLPSEKTEDKEKEKDKEEEKNEKPPDNQKLGLLEALLRISDWQHSQSIMDQLPAFYATSHKAIAVALCQLLHLIIDPLYRRAGVPKGAKGSLITPLQNKHAPQPAENFDDLSRDVFTMLCYLGPHLSHDPILFAKMVRLGKSFMKEYQSESRQDSRDKMDSLLGSFLSIADQVLLPSLSLMECNACMSEELWGFFKFFPYQHRYRLYGQWKNETYSNHPLLVKIKAQTVDRSKYIMKRLTKENVKPSGRQIGKLSHSNPTILFDYILSQIQWYDNLITPVVDSLKYLTSLNYDVLAYCIIEALANPEKEKMKHDDTTISSWLQSLASLCGAVFRKYPIELAGLLQYVTNQLKAGKSFDLLILKEVVQKMAGIEITDEMTVEQLEAMTGGEQLKAEGGYFGQIRNTKKSSQRLKDALLDHELALPLCLLMAQQRNGVVFSEGGEKHLKLVGKLYDQCHDTLVQFGGFLASNLSTEDYIKRVPSVDVLCNQLHTPHDAAFFLSRPMYAHQILSKYDELKKAEKGNRQQQKVHKYITACEQVMAPVHEAVVSLHLPKVWDDLRPQFYATFWSLTMYDLAVPHNAYDREVNKLKMQIKSIDDNTEMPPNKKKKEKERCTALQDKLQEEEKKQLEHVQRVLHRLKLEKDNWLLAKSTKNETITKFLQLCIFPRCVFSAIDAVYCARFVELVHQQKTPNFCTLLCYDRVFSDIIYTVASCTENESRRYGRFLCCMLETVTRWHSDRAIYEKECGNYPGFLTIFRASGFDGGNKADQLDYENFRHVVHKWHYKLTKASVHCLETGEYTHIRNILIVLTKILPWYPKVLNLGQALECRVHKICQEEKEKRPDLYALAMGYSGQLKGRKVHMVPENEFHHKEQPVRSTTTGTLQNGPGNIGKTSTTTAASAGKTEDGVVEDSEKSKDKSQAAQKTASKNSTTANKVSSSNGSSTPNSMKGSKEKEDKEKTTKEKKEKKEKNPGSTPEAKTGAKDKQKEERSKEEKPIDSKEKTPKADKDKRKDDKKEEKSKIDGKSIEKDWSKERDASSDSKTKESSKLEKNAGAGSLKSLVPRSEGVESEREQKRRKLDTHSSPSHSSAVKDNLNDFKDSTLKHHPNHTTVLSKIKEKDDEKKESDKSRDRSKEREKKEDRKDRKRDYLNSDREMSQEAKRRKDENGMSSSKHSKSASPSESPHSNDKDNSKRSKSTSKDKSDLAKLEKTSGGKKESRHDKEKSEKKRDSTGTKEEKKHHKSSEKHR

Target ORF information:

RefSeq Version	XM_005157134.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio THO complex 2 (thoc2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157134.3

ORF Insert Sequence:

ATGGCGGCGC TGATCCTCCC GTTTGACTGG TTAAAAAACT GGGACAAATC CGGCAGAAAT 
GAGTTTTTAC AGTTTTTTCA AGACCTTGCA GGGAAATCAA GTGGTCATGA GTTGGTTAAA 
GATCTGCAGA CAGCCTTTTA CGAGCTTTGT TGGCATGCTA TCCAGGGCAG TCTGAAGGTG 
GATGTGGCTG CTAGTCTCCT GGCTGATGTT ATGGAACTGC GGGATGATAT GCCTTCTATT 
TTAGCTGATG TTCTCTGTAT ATTAGATATT GAGACTGGTT GTTTGGAGGA GAAACACAAA 
CGGGATCTTT TTACCCAGTT GGTTGGAGCC TGTTTGATCT GTATTCCTGA TGGTGTACTA 
AAGGAGAGAT TAGATCCCGA AACACTGGAG TCTCTGGGGC TGATTAAACA AGCTCAACAG 
TTCAATCAGA AGATTGTGAA AATCAAGACC AAACTGTTTT ACAAACAGCA AAAGTTCAAT 
TTGTTGCGTG AGGAGAATGA AGGTTATGCT AAACTGATCA CAGAGCTTGG CCAAGATCTG 
ACCGGGAATC TCACCAGTCA CATCGTCCTT GAGAACATCA AGTCATTAAT AGGATGTTTC 
AATTTGGACC CAAACCGTGT GCTGGACATC ATTTTGGAGG TGTATGAATG TCGCTCTGAC 
CAGGATGAGT TTTTTATTCC TCTAATCAAG TCCTACATGT GCGAGCACCA GACCCTGTGT 
CACATGCTCG GTTTCAAGTT CAAATTCCAC CAGGAGCCTA ATGGAGAAAC ACCATCATCT 
CTGTATCACA TCGCTGCAGC TCTTCTTCAG CATGACCTCA TCGCTCTTGA AGACCTCTAT 
GTTCATCTTC TCCCACTGGA TTCCAGCATT GTGGAAGAGC ACAAGAGAGA TATCCTAGAG 
GCCAAGCAGA TTGCCCGTAA ACTCACGATG GTGGTCCTAC CCTCTGAAAA GACAGAGGAC 
AAGGAGAAAG AAAAGGACAA GGAAGAAGAA AAAAACGAGA AGCCTCCTGA TAACCAGAAG 
TTGGGACTGC TTGAGGCACT ACTGAGGATC AGTGACTGGC AGCACTCTCA GAGCATCATG 
GATCAATTGC CAGCTTTCTA TGCCACCTCC CACAAGGCCA TCGCTGTTGC ACTGTGCCAG 
CTGCTTCACC TGATCATAGA TCCACTCTAC CGCAGGGCTG GAGTACCAAA AGGTGCCAAA 
GGCAGTTTGA TAACACCTCT GCAGAACAAA CATGCACCCC AGCCCGCCGA AAACTTTGAT 
GATCTATCCA GGGATGTCTT CACCATGTTA TGCTACCTGG GACCTCACCT GTCTCATGAT 
CCCATCCTTT TTGCGAAGAT GGTGCGGCTG GGAAAGAGTT TCATGAAAGA GTACCAAAGT 
GAAAGCAGGC AAGACAGCAG GGACAAAATG GACTCTCTAC TGGGCAGTTT TCTCAGTATT 
GCTGATCAGG TGCTGTTACC ATCACTCTCG CTTATGGAGT GCAATGCCTG CATGTCTGAG 
GAACTCTGGG GCTTTTTTAA ATTCTTCCCT TACCAGCACA GGTATCGTCT CTATGGGCAA 
TGGAAAAATG AGACATACAG CAATCATCCA CTCCTGGTGA AGATCAAGGC TCAGACAGTG 
GATAGATCCA AATACATCAT GAAGCGGCTC ACCAAGGAGA ATGTTAAGCC CTCTGGAAGG 
CAGATAGGAA AGTTAAGCCA CAGCAACCCC ACCATTCTCT TCGATTATAT TCTGTCTCAG 
ATCCAGTGGT ACGACAACCT CATAACTCCA GTGGTGGACT CTCTCAAATA CTTAACATCT 
CTCAACTATG ATGTACTGGC CTATTGCATT ATTGAAGCTT TAGCCAACCC AGAGAAAGAG 
AAGATGAAAC ATGATGACAC AACCATTTCA TCATGGTTGC AGAGTCTTGC AAGTCTTTGT 
GGAGCAGTTT TCAGGAAATA TCCTATTGAG TTAGCAGGTC TTCTCCAGTA CGTAACCAAC 
CAGCTTAAAG CAGGGAAGAG TTTTGACCTG CTGATCCTGA AGGAGGTGGT GCAGAAGATG 
GCAGGAATTG AGATAACAGA TGAAATGACT GTGGAGCAGT TGGAGGCCAT GACAGGTGGA 
GAACAACTCA AAGCAGAGGG TGGTTACTTT GGACAGATTA GAAACACTAA GAAATCTTCT 
CAGCGTCTGA AGGATGCTCT TTTGGATCAT GAGCTGGCAT TACCTCTGTG TCTACTGATG 
GCCCAGCAGA GGAATGGAGT GGTGTTCTCT GAGGGGGGAG AAAAGCATCT AAAACTTGTG 
GGAAAGCTTT ATGATCAGTG TCATGACACT CTTGTGCAGT TTGGTGGCTT TTTGGCATCT 
AACCTCAGTA CAGAAGACTA CATTAAAAGG GTACCCTCAG TTGACGTCCT CTGCAACCAG 
TTACACACAC CACATGATGC TGCGTTTTTC CTGTCTCGCC CGATGTATGC CCATCAGATC 
CTGTCCAAGT ATGATGAATT GAAAAAGGCA GAGAAGGGGA ACAGGCAGCA GCAGAAAGTC 
CATAAGTACA TCACAGCCTG CGAGCAGGTT ATGGCTCCTG TTCATGAGGC TGTCGTGTCT 
CTTCATTTGC CAAAGGTGTG GGATGATCTT CGACCACAAT TCTACGCCAC ATTCTGGTCC 
CTCACCATGT ATGACCTGGC AGTACCACAC AATGCCTACG ACCGAGAAGT CAACAAGCTT 
AAGATGCAGA TCAAATCCAT TGATGATAAC ACTGAGATGC CTCCAAATAA AAAGAAAAAG 
GAGAAGGAAC GCTGTACAGC TCTGCAGGAC AAGCTACAAG AAGAGGAGAA GAAACAGTTG 
GAGCATGTGC AGAGAGTTCT GCATCGTCTC AAACTGGAGA AAGACAATTG GTTACTCGCC 
AAATCCACTA AGAATGAAAC CATCACTAAG TTCCTTCAGC TCTGCATCTT CCCCCGTTGT 
GTCTTCTCAG CCATTGATGC TGTTTACTGC GCTCGCTTTG TGGAGCTGGT TCACCAGCAG 
AAAACGCCCA ACTTCTGCAC CCTACTTTGC TATGACAGAG TCTTCTCTGA TATCATCTAT 
ACGGTAGCGA GTTGCACAGA GAATGAGTCC CGCCGATATG GTCGTTTTTT GTGTTGCATG 
TTAGAGACTG TCACTCGATG GCACAGTGAC AGAGCCATTT ATGAGAAGGA ATGTGGTAAT 
TACCCAGGGT TCCTGACTAT TTTCCGAGCC AGTGGTTTCG ACGGAGGGAA CAAAGCAGAT 
CAATTGGACT ATGAGAATTT TCGACATGTT GTCCACAAAT GGCATTACAA GTTGACTAAA 
GCATCTGTTC ACTGTCTAGA AACGGGTGAG TACACCCACA TTCGCAACAT TCTGATCGTG 
CTTACCAAGA TCCTACCCTG GTACCCCAAG GTGCTGAACC TAGGCCAGGC TTTGGAGTGT 
CGCGTTCACA AGATCTGCCA GGAAGAGAAG GAGAAGAGGC CTGATCTCTA TGCATTAGCC 
ATGGGTTACT CAGGCCAATT GAAAGGCAGA AAAGTGCATA TGGTGCCTGA AAATGAGTTC 
CACCACAAGG AGCAACCAGT ACGCAGCACC ACGACCGGCA CTCTGCAAAA TGGACCTGGG 
AATATAGGCA AGACTTCAAC CACTACCGCT GCCAGTGCTG GAAAAACTGA GGATGGTGTC 
GTCGAGGACT CAGAGAAATC CAAGGACAAA TCACAGGCAG CCCAAAAAAC TGCCAGTAAA 
AACAGCACAA CAGCCAATAA AGTGAGCAGC AGCAATGGAA GCAGCACGCC AAACAGCATG 
AAAGGGAGCA AAGAGAAGGA GGACAAAGAA AAGACCACAA AAGAGAAAAA GGAGAAAAAA 
GAAAAGAACC CAGGGAGCAC TCCAGAGGCC AAGACAGGAG CGAAAGACAA GCAAAAGGAA 
GAAAGGTCTA AGGAAGAGAA GCCCATCGAC AGCAAGGAGA AGACACCCAA GGCAGACAAG 
GACAAGAGGA AGGATGACAA GAAGGAAGAG AAGAGCAAAA TCGACGGGAA ATCCATTGAA 
AAAGATTGGT CCAAAGAACG TGATGCGTCC AGTGACAGCA AGACAAAGGA AAGCAGCAAG 
TTGGAGAAGA ATGCTGGGGC TGGAAGCCTG AAGTCCCTTG TTCCCAGATC TGAAGGAGTT 
GAGTCTGAAC GGGAGCAAAA AAGACGAAAG CTCGACACTC ACTCGTCGCC ATCACACTCC 
TCTGCAGTTA AGGACAATCT CAACGACTTC AAGGACTCCA CATTAAAGCA CCATCCTAAC 
CACACCACAG TCCTGTCCAA GATCAAAGAG AAGGATGATG AGAAGAAAGA GTCTGATAAA 
TCCAGGGACC GCTCCAAAGA GAGGGAAAAG AAAGAAGACC GGAAGGACCG AAAACGAGAC 
TACTTGAACA GCGATCGCGA GATGAGCCAG GAAGCCAAAC GCCGCAAAGA TGAAAATGGA 
ATGAGCTCAT CCAAACACAG CAAGAGTGCC AGCCCCTCTG AATCACCACA CTCCAATGAC 
AAGGACAATA GTAAACGCTC CAAGTCCACC AGCAAGGACA AGAGTGATCT GGCCAAATTG 
GAGAAGACCT CTGGAGGCAA AAAGGAATCC AGGCATGACA AAGAGAAATC AGAAAAAAAG 
AGAGACAGCA CTGGAACTAA AGAGGAGAAA AAACACCATA AATCATCAGA AAAGCACAGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Drug screening in Scn1a zebrafish mutant identifies clemizole as a potential Dravet syndrome treatment.
Nature communications42410(2013)
Baraban SC,Dinday MT,Hortopan GA

Hematopoietic gene expression profile in zebrafish kidney marrow.
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)
Song HD,Sun XJ,Deng M,Zhang GW,Zhou Y,Wu XY,Sheng Y,Chen Y,Ruan Z,Jiang CL,Fan HY,Zon LI,Kanki JP,Liu TX,Look AT,Chen Z

Identification of 315 genes essential for early zebrafish development.
Proceedings of the National Academy of Sciences of the United States of America101(35)12792-7(2004 Aug)
Amsterdam A,Nissen RM,Sun Z,Swindell EC,Farrington S,Hopkins N

Identification of nodal signaling targets by array analysis of induced complex probes.
Developmental dynamics : an official publication of the American Association of Anatomists222(4)571-80(2001 Dec)
Dickmeis T,Aanstad P,Clark M,Fischer N,Herwig R,Mourrain P,Blader P,Rosa F,Lehrach H,Strähle U

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THOC2 cDNA ORF clone, Danio rerio(Zebrafish)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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