capn8 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	capn8
Entrez Gene ID	337730
Full Name	calpain 8
Synonyms	CH73-80N6.2,hm:zehn1036,si:ch73-80n6.2,zgc:112467
General protein information	Preferred Names calpain 8 Names calpain-8
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009296382.2	XP_009294657.1	calpain-8 isoform X1
NM_001244080.1	NP_001231009.1	calpain-8

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	CAPN8	XP_426117.2
Caenorhabditis elegans (roundworm)	clp-4	NP_497460.2
Caenorhabditis elegans (roundworm)	clp-3	NP_493052.2
Caenorhabditis elegans (roundworm)	clp-7	NP_500082.1
Caenorhabditis elegans (roundworm)	clp-1	NP_498740.2
Mus musculus (house mouse)	Capn8	NP_570960.2
Homo sapiens (human)	CAPN8	NP_001137434.1
Canis lupus familiaris (dog)	CAPN8	XP_854850.3
Caenorhabditis elegans (roundworm)	clp-6	NP_500081.1
Xenopus tropicalis (tropical clawed frog)	capn8	NP_001039108.1
Xenopus tropicalis (tropical clawed frog)	LOC100497284	XP_004914783.1
Rattus norvegicus (Norway rat)	Capn8	NP_579843.2
Danio rerio (zebrafish)	capn8	NP_001231009.1

Gene Ontology
Bibliography

Related articles in PubMed

Def defines a conserved nucleolar pathway that leads p53 to proteasome-independent degradation.
Tao T, Shi H, Guan Y, Huang D, Chen Y, Lane DP, Chen J, Peng J
Cell research23(5)620-34(2013 May)

Identification of vascular and hematopoietic genes downstream of etsrp by deep sequencing in zebrafish.
Gomez G, Lee JH, Veldman MB, Lu J, Xiao X, Lin S
PloS one7(3)e31658(2012)

Discovery and characterization of novel vascular and hematopoietic genes downstream of etsrp in zebrafish.
Gomez GA, Veldman MB, Zhao Y, Burgess S, Lin S
PloS one4(3)e4994(2009)

Characterization and comparative expression of zebrafish calpain system genes during early development.
Lepage SE, Bruce AE
Developmental dynamics : an official publication of the American Association of Anatomists237(3)819-29(2008 Mar)

Identification, characterization, and mapping of expressed sequence tags from an embryonic zebrafish heart cDNA library.
Ton C, Hwang DM, Dempsey AA, Tang HC, Yoon J, Lim M, Mably JD, Fishman MC, Liew CC
Genome research10(12)1915-27(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following capn8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the capn8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa32893	NM_001244080.1 Latest version!	Danio rerio calpain 8 (capn8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa27019	XM_009296382.2 Latest version!	Danio rerio calpain 8 (capn8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

capn8 ( NM_001244080.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001231009.1 Danio rerio calpain 8 (capn8), mRNA.

Price & Availability↑

CloneID	ODa32893
Clone ID Related Accession (Same CDS sequence)	NM_001244080.1
Accession Version	NM_001244080.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450886400000
Organism	Danio rerio(zebrafish)
Product	calpain-8
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT954258.5. On Sep 15, 2011 this sequence version replaced XM_003201079.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTCTAATA TTGCAAAAGT GCTTTCCAGG AGACAGGACA GAGGAGAAGG GCTGGGGACA 
AATGAGAAGG CCATTCCCTA CAACAAGCAG GACTACCAAA GCCTGAAGCA GGAATGTCTG 
GCTAAAAGAG CCTTGTTTTG TGACCCAACT TTCCCCGCTG AATCTGACTC TCTGGGGTAC 
AATGAACTGG GACGATACTC CTTTAAAACC AAAGGAGTGC AGTGGAAGAG ACCCAAGGAG 
CTGTGCTCAA ACCCTGAGTT CATCATGGAC GGGGCCAATC GGACAGACAT CTGCCAGGGA 
GCTTTAGGAG ACTGTTGGCT TTTGGCGGCC ATTGCGTCTC TGACTCTGGA TAAGGAAATC 
CTGGAACGTG TAGTTCCATC AGGACAGAGT TTTACAGAGG ATTACGCTGG CATCTTTCAC 
TTTCAGTTTT GGCAGTATGG CGAATGGGTG GATGTGGTCA TTGATGACCG GCTGCCGACC 
AGAGATGGGA AGCTGCTGTT TGTGCACTCA GCCGAAGGCT CTGAGTTCTG GAGCGCTCTG 
TTGGAAAAAG CCTATGCAAA GGTAAATGGC TCATATGAAG CGCTGACAGG AGGTTCAACC 
ACTGAGGGAT TTGAGGATTT CACTGGAGGA ATCACTGAGA ACTATGAGCT GAGCAAAGCT 
CCTCCCAATC TGTTCAAGCT CGTACAGAAA GCACTGGCGC TGGGATCATT GCTGGGCTGC 
TCTATTGACA TTACCAATTC ATATGAGACT GAGGCAGTGA CGAGTCTGAA GCTGGTGAAG 
GGACATGCAT ACTCAGTCAC GGCTGCAGAG GAGGTTCACT TCAGAGGCAG TCTGGTTCAG 
CTGGTGCGAA TCAGGAACCC GTGGGGTGAG GTGGAGTGGA CGGGGGCCTG GAGCGACAAT 
TCCAAAGAGT GGGACAGTGT CCGACCAGAA GAGAAAGCTA AACTGGATTA TTCAGCTGAA 
GATGGAGAGT TCTGGATGGC GTATTCTGAC TTCATACAGC AGTTCTCCAA GTTGGAGATC 
TGTAATCTGA CTCCTGACAC TCTTTCGAGT GAAGAAGTGA ATCGCTGGAG CTACAGTCAG 
TTTGAAGGCA ACTGGAGGGT GGGCTCCACT GCGGGAGGCT GCAGGAACAA CCCTGCAACG 
TTCTGCTCAA ACCCTCAGTT TATGATCAAG CTGGAGGAAA TAGATGATGA TCCTCATGAC 
GGAGTGAACG GCTGCACATT CCTAGTGGGT TTGATACAGA AAGATGGTCG TAAAGACAAG 
CGCTTTGGAC GAGACCTAAA CACCATCGGC TTTGCCATCT ATAAGGTTCC GGATGAGTTA 
AAGGGTCGCA GCAATATTCA TCTGGGCCCT GATGTCCTGC TGCGTCAGCG TTCAGTTGCT 
GGGAGCAACA CCTTCATCAA CCTGCGAGAG GTGAGCGAGC GCTTCAAACT GCCTCCGGGA 
GAATACATCA TCATCCCCTC CACCTTTGAA CCGCACCGCA AAGGAAGTTT CATTCTGCGG 
GTGTTCGCAG AAAAAGAGGC AGATGCTAGC CAGATTGGTA CAGAGATCAG TGCAGATGTG 
AAAAAGCATT TCATATCTGA GAAAGATATA GACCCACATT TCAAACGCCT CTTCAGTCAG 
GTTGCTGGAA GCGACTCTGA GGTCTCTGTA TTGGAGCTCC AGCAGATCCT GAACACCGTT 
GTCTCGAAAC GTAAATCTAA TGTGAAGACA GACGGATTCA GTTTGGAAAC CTGCCGTCAC 
ATTATCAGCC TGCTAGATAA AGATGGCAGT GGCAAACTCG GCCTTCTGGA GTTTCACACA 
CTGTGGATGA AGATCCAGAA ATATTTGGAA ATTTTTAAGC ACCGCGATAC AGACAACTCA 
GGTACCATGA GTTCTCTTGA GATGCGAGAT GCGGTAAAAG AAGCAGGGTT CCAGCTGAAC 
AATGATGTAC TCGAGGTGCT GATCGCACGA TATGCCAACC AGGAATACGC CATTGACTTT 
GATAGCTTCG TCAGCTGCCT GATCCGCCTC GAGCTGCTCT TCAAAATGTT TCAACTGTTC 
GATAAGAAGA ACACAGGGAA AATCGAACTG GATATCCTGC AGTGGCTCTG CCTGGCCCTC 
AGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001231009.1
CDS	58..2163
Translation	MSNIAKVLSRRQDRGEGLGTNEKAIPYNKQDYQSLKQECLAKRALFCDPTFPAESDSLGYNELGRYSFKTKGVQWKRPKELCSNPEFIMDGANRTDICQGALGDCWLLAAIASLTLDKEILERVVPSGQSFTEDYAGIFHFQFWQYGEWVDVVIDDRLPTRDGKLLFVHSAEGSEFWSALLEKAYAKVNGSYEALTGGSTTEGFEDFTGGITENYELSKAPPNLFKLVQKALALGSLLGCSIDITNSYETEAVTSLKLVKGHAYSVTAAEEVHFRGSLVQLVRIRNPWGEVEWTGAWSDNSKEWDSVRPEEKAKLDYSAEDGEFWMAYSDFIQQFSKLEICNLTPDTLSSEEVNRWSYSQFEGNWRVGSTAGGCRNNPATFCSNPQFMIKLEEIDDDPHDGVNGCTFLVGLIQKDGRKDKRFGRDLNTIGFAIYKVPDELKGRSNIHLGPDVLLRQRSVAGSNTFINLREVSERFKLPPGEYIIIPSTFEPHRKGSFILRVFAEKEADASQIGTEISADVKKHFISEKDIDPHFKRLFSQVAGSDSEVSVLELQQILNTVVSKRKSNVKTDGFSLETCRHIISLLDKDGSGKLGLLEFHTLWMKIQKYLEIFKHRDTDNSGTMSSLEMRDAVKEAGFQLNNDVLEVLIARYANQEYAIDFDSFVSCLIRLELLFKMFQLFDKKNTGKIELDILQWLCLALS

Target ORF information:

RefSeq Version	NM_001244080.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpain 8 (capn8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001244080.1

ORF Insert Sequence:

ATGTCTAATA TTGCAAAAGT GCTTTCCAGG AGACAGGACA GAGGAGAAGG GCTGGGGACA 
AATGAGAAGG CCATTCCCTA CAACAAGCAG GACTACCAAA GCCTGAAGCA GGAATGTCTG 
GCTAAAAGAG CCTTGTTTTG TGACCCAACT TTCCCCGCTG AATCTGACTC TCTGGGGTAC 
AATGAACTGG GACGATACTC CTTTAAAACC AAAGGAGTGC AGTGGAAGAG ACCCAAGGAG 
CTGTGCTCAA ACCCTGAGTT CATCATGGAC GGGGCCAATC GGACAGACAT CTGCCAGGGA 
GCTTTAGGAG ACTGTTGGCT TTTGGCGGCC ATTGCGTCTC TGACTCTGGA TAAGGAAATC 
CTGGAACGTG TAGTTCCATC AGGACAGAGT TTTACAGAGG ATTACGCTGG CATCTTTCAC 
TTTCAGTTTT GGCAGTATGG CGAATGGGTG GATGTGGTCA TTGATGACCG GCTGCCGACC 
AGAGATGGGA AGCTGCTGTT TGTGCACTCA GCCGAAGGCT CTGAGTTCTG GAGCGCTCTG 
TTGGAAAAAG CCTATGCAAA GGTAAATGGC TCATATGAAG CGCTGACAGG AGGTTCAACC 
ACTGAGGGAT TTGAGGATTT CACTGGAGGA ATCACTGAGA ACTATGAGCT GAGCAAAGCT 
CCTCCCAATC TGTTCAAGCT CGTACAGAAA GCACTGGCGC TGGGATCATT GCTGGGCTGC 
TCTATTGACA TTACCAATTC ATATGAGACT GAGGCAGTGA CGAGTCTGAA GCTGGTGAAG 
GGACATGCAT ACTCAGTCAC GGCTGCAGAG GAGGTTCACT TCAGAGGCAG TCTGGTTCAG 
CTGGTGCGAA TCAGGAACCC GTGGGGTGAG GTGGAGTGGA CGGGGGCCTG GAGCGACAAT 
TCCAAAGAGT GGGACAGTGT CCGACCAGAA GAGAAAGCTA AACTGGATTA TTCAGCTGAA 
GATGGAGAGT TCTGGATGGC GTATTCTGAC TTCATACAGC AGTTCTCCAA GTTGGAGATC 
TGTAATCTGA CTCCTGACAC TCTTTCGAGT GAAGAAGTGA ATCGCTGGAG CTACAGTCAG 
TTTGAAGGCA ACTGGAGGGT GGGCTCCACT GCGGGAGGCT GCAGGAACAA CCCTGCAACG 
TTCTGCTCAA ACCCTCAGTT TATGATCAAG CTGGAGGAAA TAGATGATGA TCCTCATGAC 
GGAGTGAACG GCTGCACATT CCTAGTGGGT TTGATACAGA AAGATGGTCG TAAAGACAAG 
CGCTTTGGAC GAGACCTAAA CACCATCGGC TTTGCCATCT ATAAGGTTCC GGATGAGTTA 
AAGGGTCGCA GCAATATTCA TCTGGGCCCT GATGTCCTGC TGCGTCAGCG TTCAGTTGCT 
GGGAGCAACA CCTTCATCAA CCTGCGAGAG GTGAGCGAGC GCTTCAAACT GCCTCCGGGA 
GAATACATCA TCATCCCCTC CACCTTTGAA CCGCACCGCA AAGGAAGTTT CATTCTGCGG 
GTGTTCGCAG AAAAAGAGGC AGATGCTAGC CAGATTGGTA CAGAGATCAG TGCAGATGTG 
AAAAAGCATT TCATATCTGA GAAAGATATA GACCCACATT TCAAACGCCT CTTCAGTCAG 
GTTGCTGGAA GCGACTCTGA GGTCTCTGTA TTGGAGCTCC AGCAGATCCT GAACACCGTT 
GTCTCGAAAC GTAAATCTAA TGTGAAGACA GACGGATTCA GTTTGGAAAC CTGCCGTCAC 
ATTATCAGCC TGCTAGATAA AGATGGCAGT GGCAAACTCG GCCTTCTGGA GTTTCACACA 
CTGTGGATGA AGATCCAGAA ATATTTGGAA ATTTTTAAGC ACCGCGATAC AGACAACTCA 
GGTACCATGA GTTCTCTTGA GATGCGAGAT GCGGTAAAAG AAGCAGGGTT CCAGCTGAAC 
AATGATGTAC TCGAGGTGCT GATCGCACGA TATGCCAACC AGGAATACGC CATTGACTTT 
GATAGCTTCG TCAGCTGCCT GATCCGCCTC GAGCTGCTCT TCAAAATGTT TCAACTGTTC 
GATAAGAAGA ACACAGGGAA AATCGAACTG GATATCCTGC AGTGGCTCTG CCTGGCCCTC 
AGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

capn8 ( XM_009296382.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009294657.1 Danio rerio calpain 8 (capn8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa27019
Clone ID Related Accession (Same CDS sequence)	XM_009296382.2
Accession Version	XM_009296382.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	calpain-8 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296382.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAATA TTGCAAAAGT GCTTTCCAGG AGACAGGACA GAGGAGAAGG GCTGGGGACA 
AATGAGAAGG CCATTCCCTA CAACAAGCAG GACTACCAAA GCCTGAAGCA GGAATGTCTG 
GCTAAAAGAG CCTTGTTTTG TGACCCAACT TTCCCCGCTG AATCTGACTC TCTGGGGTAC 
AATGAACTGG GACGATACTC CTTTAAAACC AAAGGAGTGC AGTGGAAGAG ACCCAAGGAG 
CTGTGCTCAA ACCCTGAGTT CATCATGGAC GGGGCCAATC GGACAGACAT CTGCCAGGGA 
GCTTTAGGAG ACTGTTGGCT TTTGGCGGCC ATTGCGTCTC TGACTCTGGA TAAGGAAATC 
CTGGAACGTG TAGTTCCATC AGGACAGAGT TTTACAGAGG ATTACGCTGG CATCTTTCAC 
TTTCAGTTTT GGCAGTATGG CGAATGGGTG GATGTGGTCA TTGATGACCG GCTGCCGACC 
AGAGATGGGA AGCTGCTGTT TGTGCACTCA GCCGAAGGCT CTGAGTTCTG GAGCGCTCTG 
TTGGAAAAAG CCTATGCAAA GGTAAATGGC TCATATGAAG CGCTGACAGG AGGTTCAACC 
ACTGAGGGAT TTGAGGATTT CACTGGAGGA ATCACTGAGA ACTATGAGCT GAGCAAAGCT 
CCTCCCAATC TGTTCAAGCT CGTACAGAAA GCACTGGCGC TGGGATCATT GCTGGGCTGC 
TCTATTGACA TTACCAATTC ATATGAGACT GAGGCAGTGA CGAGTCTGAA GCTGGTGAAG 
GGACATGCAT ACTCAGTCAC GGCTGCAGAG GAGGTTCACT TCAGAGGCAG TCTGGTTCAG 
CTGGTGCGAA TCAGGAACCC GTGGGGTGAG GTGGAGTGGA CGGGGGCCTG GAGCGACAAT 
TCCAAAGAGT GGGACAGTGT CCGACCAGAA GAGAAAGCTA AACTGGATTA TTCAGCTGAA 
GATGGAGAGT TCTGGATGGC GTATTCTGAC TTCATACAGC AGTTCTCCAA GTTGGAGATC 
TGTAATCTGA CTCCTGACAC TCTTTCGAGT GAAGAAGTGA ATCGCTGGAG CTACAGTCAG 
TTTGAAGGCA ACTGGAGGGT GGGCTCCACT GCGGGAGGCT GCAGGAACAA CCCTGCAACG 
TTCTGCTCAA ACCCTCAGTT TATGATCAAG CTGGAGGAAA TAGATGATGA TCCTCATGAC 
GGAGTGAACG GCTGCACATT CCTAGTGGGT TTGATACAGA AAGATGGTCG TAAAGACAAG 
CGCTTTGGAC GAGACCTAAA CACCATCGGC TTTGCCATCT ATAAGGTTCC GGATGAGTTA 
AAGGGTCGCA GCAATATTCA TCTGGGCCCT GATGTCCTGC TGCGTCAGCG TTCAGTTGCT 
GGGAGCAACA CCTTCATCAA CCTGCGAGAG GTGAGCGAGC GCTTCAAACT GCCTCCGGGA 
GAATACATCA TCATCCCCTC CACCTTTGAA CCGCACCGCA AAGGAAGTTT CATTCTGCGG 
GTGTTCGCAG AAAAAGAGGC AGATGCTAGC CAGATTGGTA CAGAGATCAG TGCAGATGTG 
AAAAAGCATT TCATATCTGA GAAAGATATA GACCCACATT TCAAACGCCT CTTCAGTCAG 
GTTGCTGGAA GCGACTCTGA GGTCTCTGTA TTGGAGCTCC AGCAGATCCT GAACACCGTT 
GTCTCGAAAC GATCTAATGT GAAGACAGAC GGATTCAGTT TGGAAACCTG CCGTCACATT 
ATCAGCCTGC TAGATAAAGA TGGCAGTGGC AAACTCGGCC TTCTGGAGTT TCACACACTG 
TGGATGAAGA TCCAGAAATA TTTGGAAATT TTTAAGCACC GCGATACAGA CAACTCAGGT 
ACCATGAGTT CTCTTGAGAT GCGAGATGCG GTAAAAGAAG CAGGGTTCCA GCTGAACAAT 
GATGTACTCG AGGTGCTGAT CGCACGATAT GCCAACCAGG AATACGCCAT TGACTTTGAT 
AGCTTCGTCA GCTGCCTGAT CCGCCTCGAG CTGCTCTTCA AAATGTTTCA ACTGTTCGAT 
AAGAAGAACA CAGGGAAAAT CGAACTGGAT ATCCTGCAGT GGCTCTGCCT GGCCCTCAGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294657.1
CDS	128..2230
Translation	MSNIAKVLSRRQDRGEGLGTNEKAIPYNKQDYQSLKQECLAKRALFCDPTFPAESDSLGYNELGRYSFKTKGVQWKRPKELCSNPEFIMDGANRTDICQGALGDCWLLAAIASLTLDKEILERVVPSGQSFTEDYAGIFHFQFWQYGEWVDVVIDDRLPTRDGKLLFVHSAEGSEFWSALLEKAYAKVNGSYEALTGGSTTEGFEDFTGGITENYELSKAPPNLFKLVQKALALGSLLGCSIDITNSYETEAVTSLKLVKGHAYSVTAAEEVHFRGSLVQLVRIRNPWGEVEWTGAWSDNSKEWDSVRPEEKAKLDYSAEDGEFWMAYSDFIQQFSKLEICNLTPDTLSSEEVNRWSYSQFEGNWRVGSTAGGCRNNPATFCSNPQFMIKLEEIDDDPHDGVNGCTFLVGLIQKDGRKDKRFGRDLNTIGFAIYKVPDELKGRSNIHLGPDVLLRQRSVAGSNTFINLREVSERFKLPPGEYIIIPSTFEPHRKGSFILRVFAEKEADASQIGTEISADVKKHFISEKDIDPHFKRLFSQVAGSDSEVSVLELQQILNTVVSKRSNVKTDGFSLETCRHIISLLDKDGSGKLGLLEFHTLWMKIQKYLEIFKHRDTDNSGTMSSLEMRDAVKEAGFQLNNDVLEVLIARYANQEYAIDFDSFVSCLIRLELLFKMFQLFDKKNTGKIELDILQWLCLALS

Target ORF information:

RefSeq Version	XM_009296382.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calpain 8 (capn8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296382.2

ORF Insert Sequence:

ATGTCTAATA TTGCAAAAGT GCTTTCCAGG AGACAGGACA GAGGAGAAGG GCTGGGGACA 
AATGAGAAGG CCATTCCCTA CAACAAGCAG GACTACCAAA GCCTGAAGCA GGAATGTCTG 
GCTAAAAGAG CCTTGTTTTG TGACCCAACT TTCCCCGCTG AATCTGACTC TCTGGGGTAC 
AATGAACTGG GACGATACTC CTTTAAAACC AAAGGAGTGC AGTGGAAGAG ACCCAAGGAG 
CTGTGCTCAA ACCCTGAGTT CATCATGGAC GGGGCCAATC GGACAGACAT CTGCCAGGGA 
GCTTTAGGAG ACTGTTGGCT TTTGGCGGCC ATTGCGTCTC TGACTCTGGA TAAGGAAATC 
CTGGAACGTG TAGTTCCATC AGGACAGAGT TTTACAGAGG ATTACGCTGG CATCTTTCAC 
TTTCAGTTTT GGCAGTATGG CGAATGGGTG GATGTGGTCA TTGATGACCG GCTGCCGACC 
AGAGATGGGA AGCTGCTGTT TGTGCACTCA GCCGAAGGCT CTGAGTTCTG GAGCGCTCTG 
TTGGAAAAAG CCTATGCAAA GGTAAATGGC TCATATGAAG CGCTGACAGG AGGTTCAACC 
ACTGAGGGAT TTGAGGATTT CACTGGAGGA ATCACTGAGA ACTATGAGCT GAGCAAAGCT 
CCTCCCAATC TGTTCAAGCT CGTACAGAAA GCACTGGCGC TGGGATCATT GCTGGGCTGC 
TCTATTGACA TTACCAATTC ATATGAGACT GAGGCAGTGA CGAGTCTGAA GCTGGTGAAG 
GGACATGCAT ACTCAGTCAC GGCTGCAGAG GAGGTTCACT TCAGAGGCAG TCTGGTTCAG 
CTGGTGCGAA TCAGGAACCC GTGGGGTGAG GTGGAGTGGA CGGGGGCCTG GAGCGACAAT 
TCCAAAGAGT GGGACAGTGT CCGACCAGAA GAGAAAGCTA AACTGGATTA TTCAGCTGAA 
GATGGAGAGT TCTGGATGGC GTATTCTGAC TTCATACAGC AGTTCTCCAA GTTGGAGATC 
TGTAATCTGA CTCCTGACAC TCTTTCGAGT GAAGAAGTGA ATCGCTGGAG CTACAGTCAG 
TTTGAAGGCA ACTGGAGGGT GGGCTCCACT GCGGGAGGCT GCAGGAACAA CCCTGCAACG 
TTCTGCTCAA ACCCTCAGTT TATGATCAAG CTGGAGGAAA TAGATGATGA TCCTCATGAC 
GGAGTGAACG GCTGCACATT CCTAGTGGGT TTGATACAGA AAGATGGTCG TAAAGACAAG 
CGCTTTGGAC GAGACCTAAA CACCATCGGC TTTGCCATCT ATAAGGTTCC GGATGAGTTA 
AAGGGTCGCA GCAATATTCA TCTGGGCCCT GATGTCCTGC TGCGTCAGCG TTCAGTTGCT 
GGGAGCAACA CCTTCATCAA CCTGCGAGAG GTGAGCGAGC GCTTCAAACT GCCTCCGGGA 
GAATACATCA TCATCCCCTC CACCTTTGAA CCGCACCGCA AAGGAAGTTT CATTCTGCGG 
GTGTTCGCAG AAAAAGAGGC AGATGCTAGC CAGATTGGTA CAGAGATCAG TGCAGATGTG 
AAAAAGCATT TCATATCTGA GAAAGATATA GACCCACATT TCAAACGCCT CTTCAGTCAG 
GTTGCTGGAA GCGACTCTGA GGTCTCTGTA TTGGAGCTCC AGCAGATCCT GAACACCGTT 
GTCTCGAAAC GATCTAATGT GAAGACAGAC GGATTCAGTT TGGAAACCTG CCGTCACATT 
ATCAGCCTGC TAGATAAAGA TGGCAGTGGC AAACTCGGCC TTCTGGAGTT TCACACACTG 
TGGATGAAGA TCCAGAAATA TTTGGAAATT TTTAAGCACC GCGATACAGA CAACTCAGGT 
ACCATGAGTT CTCTTGAGAT GCGAGATGCG GTAAAAGAAG CAGGGTTCCA GCTGAACAAT 
GATGTACTCG AGGTGCTGAT CGCACGATAT GCCAACCAGG AATACGCCAT TGACTTTGAT 
AGCTTCGTCA GCTGCCTGAT CCGCCTCGAG CTGCTCTTCA AAATGTTTCA ACTGTTCGAT 
AAGAAGAACA CAGGGAAAAT CGAACTGGAT ATCCTGCAGT GGCTCTGCCT GGCCCTCAGT 
TGA