b3gnt5a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	b3gnt5a
Entrez Gene ID	336526
Full Name	UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5a
Synonyms	Lc3,b3gnt5,fb12f12,wu:fb12f12,wu:fe50g08
General protein information	Preferred Names UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5a Names lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase A BGnT-5A UDP-GlcNAc:beta-Gal beta-1,3-N-acetylglucosaminyltransferase 5A UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 beta-1,3-Gn-T5A beta-1,3-N-acetylglucosaminyltransferase 5A beta3Gn-T5A lactotriaosylceramide synthase A lc(3)Cer synthase A lc3 synthase A
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_198876.1	NP_942577.1	lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase A precursor

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	B3GNT5	NP_001107233.1
Danio rerio (zebrafish)	b3gnt5a	NP_942577.1
Xenopus tropicalis (tropical clawed frog)	b3gnt5	NP_989240.1
Canis lupus familiaris (dog)	B3GNT5	XP_005639817.1
Macaca mulatta (Rhesus monkey)	B3GNT5	XP_001106314.1
Homo sapiens (human)	B3GNT5	NP_114436.1
Pan troglodytes (chimpanzee)	B3GNT5	XP_001134746.2
Bos taurus (cattle)	B3GNT5	NP_001070447.1
Mus musculus (house mouse)	B3gnt5	NP_001152879.1
Rattus norvegicus (Norway rat)	B3gnt5	NP_446384.1

Gene Ontology
Bibliography

Related articles in PubMed

Crosstalk of oxidative damage, apoptosis, and autophagy under endoplasmic reticulum (ER) stress involved in thifluzamide-induced liver damage in zebrafish (Danio rerio).
Yang Y, Dong F, Liu X, Xu J, Wu X, Liu W, Zheng Y
Environmental pollution (Barking, Essex : 1987)243(Pt B)1904-1911(2018 Dec)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Fgf19 is required for zebrafish lens and retina development.
Nakayama Y, Miyake A, Nakagawa Y, Mido T, Yoshikawa M, Konishi M, Itoh N
Developmental biology313(2)752-66(2008 Jan)

Expression of zebrafish Lc3 synthase gene in embryonic lens requires hedgehog signaling.
Cao Y, Zhao J, Wang Y, Meng A
Developmental dynamics : an official publication of the American Association of Anatomists228(3)308-12(2003 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following b3gnt5a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the b3gnt5a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa37736	NM_198876.1 Latest version!	Danio rerio UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5a (b3gnt5a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

b3gnt5a ( NM_198876.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_942577.1 Danio rerio UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5a (b3gnt5a), mRNA.

Price & Availability↑

CloneID	ODa37736
Clone ID Related Accession (Same CDS sequence)	NM_198876.1
Accession Version	NM_198876.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577203200000
Organism	Danio rerio(zebrafish)
Product	lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase A precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY292460.1. ##Evidence-Data-START## Transcript exon combination :: AY292460.1, CN020622.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTTCATGA ATTGCAGAAG GGTGAAAAAA TGGCATTTCC TCCAGCTTCT CTCGATGTGC 
TGTGTCATGT CAGTCCTTAT GGTTTGCTGG GAGCACGTGG ACCATCACGT AGTGAGCCAT 
GTGAAATCCT ACTCCTACCG CTACCTAATC AACAGCTACG ACTTCATCAA CAAGAGCCTC 
AGCGTCAGCC CGGAGGAAGC CGCCCGGTTT GGTAGCTTCC CGTATTTGCT TGACCGCAGG 
GACGTTTGTA AAAACAAAGA CGTCCTGCTT TTGCTCTTTG TCAAGTCTTC TCCAGGAAAC 
TTCAAAAGGC GGCAGGCCAT ACGTTCCACT TGGGGCAACG AGTCCTATAT TAGTCAGGAA 
CTCGGTGTTG TCGTCAAGGT AGTGTTTGCT ATGGGAGTTC GCCCTGACCG ATCGGGACAT 
AAAACAATGC AGAGGGAACT GCGCAAGGAG CACATGGCCC ACCATGACCT GATTCAACAG 
GACTTCCTCG ACACATTCCA CAACCTCACT GTGAAACTGC TGCTTCAGTT CCGCTGGACG 
CACGAAAACT GCGCACATGC CCACTTCCTT ATGAGTGCAG ACGATGATGT CTTCATCCAT 
GTGCCTAACC TAGTGCACTA CCTCCAGGAG CTTAAAAGTC AGAACGTACG CAACCTCTGG 
GTCGGCCACG TTCATAGGGG GGCACCGCCT GTTCGGAAGC GGGACAGCAA GTATTATATG 
CCATTTGATA TGTATCAGTG GTCCTCCTAC CCAGATTACA CTGCTGGCGC CGGGTATGTC 
GTTTCTGGCG ACGTGGCAGC CAAAATCTAC CAGGCCACGC AGTCTCTAAA CGCCTCAATG 
TATATCGATG ACGTCTTCAT GGGCATCTGT GCCATCGCTG CGGGCGTCTC GCCTCAGGAA 
CATGTTTACT TCTCGGGTGA GGGGAAAACG CCCTATCACC CGTGCATCTA CGAAAAGATG 
ATCACCTCTC ACGGACACGA AGGCGATATC AGGTACCTGT GGAAGGCTGC GACCGGCCCG 
CAGGTAGAAG GCATTTCATC TGGATTGTTG GGGAAGTTGT ATTGCGCAGC TGTGAAAATG 
ACGCTGCTTT GTAAGCCGTA CTTTACGAAC ACGTATTCAT GCATGGCAGC TTTTACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_942577.1
CDS	403..1542
Translation	MFMNCRRVKKWHFLQLLSMCCVMSVLMVCWEHVDHHVVSHVKSYSYRYLINSYDFINKSLSVSPEEAARFGSFPYLLDRRDVCKNKDVLLLLFVKSSPGNFKRRQAIRSTWGNESYISQELGVVVKVVFAMGVRPDRSGHKTMQRELRKEHMAHHDLIQQDFLDTFHNLTVKLLLQFRWTHENCAHAHFLMSADDDVFIHVPNLVHYLQELKSQNVRNLWVGHVHRGAPPVRKRDSKYYMPFDMYQWSSYPDYTAGAGYVVSGDVAAKIYQATQSLNASMYIDDVFMGICAIAAGVSPQEHVYFSGEGKTPYHPCIYEKMITSHGHEGDIRYLWKAATGPQVEGISSGLLGKLYCAAVKMTLLCKPYFTNTYSCMAAFT

Target ORF information:

RefSeq Version	NM_198876.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5a (b3gnt5a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198876.1

ORF Insert Sequence:

ATGTTCATGA ATTGCAGAAG GGTGAAAAAA TGGCATTTCC TCCAGCTTCT CTCGATGTGC 
TGTGTCATGT CAGTCCTTAT GGTTTGCTGG GAGCACGTGG ACCATCACGT AGTGAGCCAT 
GTGAAATCCT ACTCCTACCG CTACCTAATC AACAGCTACG ACTTCATCAA CAAGAGCCTC 
AGCGTCAGCC CGGAGGAAGC CGCCCGGTTT GGTAGCTTCC CGTATTTGCT TGACCGCAGG 
GACGTTTGTA AAAACAAAGA CGTCCTGCTT TTGCTCTTTG TCAAGTCTTC TCCAGGAAAC 
TTCAAAAGGC GGCAGGCCAT ACGTTCCACT TGGGGCAACG AGTCCTATAT TAGTCAGGAA 
CTCGGTGTTG TCGTCAAGGT AGTGTTTGCT ATGGGAGTTC GCCCTGACCG ATCGGGACAT 
AAAACAATGC AGAGGGAACT GCGCAAGGAG CACATGGCCC ACCATGACCT GATTCAACAG 
GACTTCCTCG ACACATTCCA CAACCTCACT GTGAAACTGC TGCTTCAGTT CCGCTGGACG 
CACGAAAACT GCGCACATGC CCACTTCCTT ATGAGTGCAG ACGATGATGT CTTCATCCAT 
GTGCCTAACC TAGTGCACTA CCTCCAGGAG CTTAAAAGTC AGAACGTACG CAACCTCTGG 
GTCGGCCACG TTCATAGGGG GGCACCGCCT GTTCGGAAGC GGGACAGCAA GTATTATATG 
CCATTTGATA TGTATCAGTG GTCCTCCTAC CCAGATTACA CTGCTGGCGC CGGGTATGTC 
GTTTCTGGCG ACGTGGCAGC CAAAATCTAC CAGGCCACGC AGTCTCTAAA CGCCTCAATG 
TATATCGATG ACGTCTTCAT GGGCATCTGT GCCATCGCTG CGGGCGTCTC GCCTCAGGAA 
CATGTTTACT TCTCGGGTGA GGGGAAAACG CCCTATCACC CGTGCATCTA CGAAAAGATG 
ATCACCTCTC ACGGACACGA AGGCGATATC AGGTACCTGT GGAAGGCTGC GACCGGCCCG 
CAGGTAGAAG GCATTTCATC TGGATTGTTG GGGAAGTTGT ATTGCGCAGC TGTGAAAATG 
ACGCTGCTTT GTAAGCCGTA CTTTACGAAC ACGTATTCAT GCATGGCAGC TTTTACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Crosstalk of oxidative damage, apoptosis, and autophagy under endoplasmic reticulum (ER) stress involved in thifluzamide-induced liver damage in zebrafish (Danio rerio).
Environmental pollution (Barking, Essex : 1987)243(Pt B)1904-1911(2018 Dec)
Yang Y,Dong F,Liu X,Xu J,Wu X,Liu W,Zheng Y

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Fgf19 is required for zebrafish lens and retina development.
Developmental biology313(2)752-66(2008 Jan)
Nakayama Y,Miyake A,Nakagawa Y,Mido T,Yoshikawa M,Konishi M,Itoh N

Expression of zebrafish Lc3 synthase gene in embryonic lens requires hedgehog signaling.
Developmental dynamics : an official publication of the American Association of Anatomists228(3)308-12(2003 Nov)
Cao Y,Zhao J,Wang Y,Meng A

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b3gnt5a cDNA ORF clone, Danio rerio(zebrafish)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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