nme2a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	nme2a
Entrez Gene ID	335716
Full Name	NME/NM23 nucleoside diphosphate kinase 2a
Synonyms	nme2l,si:ch211-119c20.1,wu:fk59e05,zgc:73122
General protein information	Preferred Names NME/NM23 nucleoside diphosphate kinase 2a Names nucleoside diphosphate kinase B non-metastatic cells 2-like, protein (NM23B) expressed in non-metastatic cells 2a, protein (NM23B) expressed in novel nucleoside-diphosphate kinase (wu:fk59e05)
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005160367.1	XP_005160424.1	nucleoside diphosphate kinase B isoform X1
NM_199970.2	NP_956264.1	nucleoside diphosphate kinase B
XM_021480031.1	XP_021335706.1	nucleoside diphosphate kinase B isoform X1

Pathways from BioSystems

Homologies

Neurospora crassa	NCU04202	XP_961162.1
Caenorhabditis elegans (roundworm)	ndk-1	NP_492761.1
Bos taurus (cattle)	LOC101905864	XP_005220712.1
Macaca mulatta (Rhesus monkey)	LOC707717	XP_001096144.2
Mus musculus (house mouse)	Gm5566	XP_006505013.1
Oryza sativa (rice)	Os10g0563700	NP_001065404.1
Drosophila melanogaster (fruit fly)	awd	NP_476761.2
Arabidopsis thaliana (thale cress)	NDPK1	NP_567346.1
Magnaporthe oryzae (rice blast fungus)	MGG_08622	XP_003711050.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007120	XP_308641.4
Homo sapiens (human)	NME1-NME2	NP_001018146.1
Danio rerio (zebrafish)	nme2a	NP_956264.1
Pan troglodytes (chimpanzee)	LOC101059613	XP_003952370.1
Kluyveromyces lactis	KLLA0D03696g	XP_453229.1
Schizosaccharomyces pombe (fission yeast)	ndk1	NP_592857.1
Eremothecium gossypii	AGOS_ABR096C	NP_983042.2
Saccharomyces cerevisiae (baker's yeast)	YNK1	NP_012856.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Nucleoside diphosphate kinase B regulates angiogenesis through modulation of vascular endothelial growth factor receptor type 2 and endothelial adherens junction proteins.
Feng Y, Gross S, Wolf NM, Butenschön VM, Qiu Y, Devraj K, Liebner S, Kroll J, Skolnik EY, Hammes HP, Wieland T
Arteriosclerosis, thrombosis, and vascular biology34(10)2292-300(2014 Oct)

Irx7, a Smarca4-regulated gene for retinal differentiation, regulates other genes controlled by Smarca4 in zebrafish retinas.
Zhang Y, Bonilla S, Chong L, Leung YF
Gene expression patterns : GEP13(5-6)177-82(2013 Jun-Jul)

Cellular expression of Smarca4 (Brg1)-regulated genes in zebrafish retinas.
Hensley MR, Emran F, Bonilla S, Zhang L, Zhong W, Grosu P, Dowling JE, Leung YF
BMC developmental biology1145(2011 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following nme2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nme2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa98305	NM_199970.2 Latest version!	Danio rerio NME/NM23 nucleoside diphosphate kinase 2a (nme2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
ODa148141	XM_021480031.1 Latest version!	Danio rerio NME/NM23 nucleoside diphosphate kinase 2a (nme2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
ODa17841	XM_005160367.1 Latest version!	Danio rerio NME/NM23 nucleoside diphosphate kinase 2a (nme2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nme2a ( NM_199970.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956264.1 Danio rerio NME/NM23 nucleoside diphosphate kinase 2a (nme2a), mRNA.

Price & Availability↑

CloneID	ODa98305
Clone ID Related Accession (Same CDS sequence)	NM_199970.2
Accession Version	NM_199970.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1542902400000
Organism	Danio rerio(zebrafish)
Product	nucleoside diphosphate kinase B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX004803.11. On Jul 8, 2016 this sequence version replaced NM_199970.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: CN503273.1, EH442630.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505370, SAMEA3505371 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END##

ATGTCTGGGA ATGAAGAGCG CACTTTCATC GCCATCAAGC CGGATGGGGT GCAGAGAGGA 
CTCGTCGGAG AAATCATCAA GCGCTTCGAA CAGAAAGGCT TCAAGTTAGT GGCTATGAAG 
TTAATCCAGG CAGATGAGGA TCTTCTTAGA CAGCACTACA GTGACCTGAA GGATCGGCCT 
TTTTTCCCTG GACTTGTCAG TTATATGTCT GCTGGTCCTG TGGTTGCAAT GGTGTGGGAA 
GGTTTTAATG TTATAAAAAC TGGCAGAGTA ATGCTTGGCG AGACTAATCC CATTGACTCC 
AAGCCTGGGA CTATCCGAGG TGACTTTTGC GTTCAAGTTG GACGGAACAT TATTCACGGC 
AGTGATTCTG TGGAAAGTGC CAACACTGAG ATCAACCTGT GGTTCAAACC AGATGAGATC 
TGCGATTACA CCAAATGTCA GGAAAGCTGG ATCAACAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956264.1
CDS	85..546
Translation	MSGNEERTFIAIKPDGVQRGLVGEIIKRFEQKGFKLVAMKLIQADEDLLRQHYSDLKDRPFFPGLVSYMSAGPVVAMVWEGFNVIKTGRVMLGETNPIDSKPGTIRGDFCVQVGRNIIHGSDSVESANTEINLWFKPDEICDYTKCQESWINS

Target ORF information:

RefSeq Version	NM_199970.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NME/NM23 nucleoside diphosphate kinase 2a (nme2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199970.2

ORF Insert Sequence:

ATGTCTGGGA ATGAAGAGCG CACTTTCATC GCCATCAAGC CGGATGGGGT GCAGAGAGGA 
CTCGTCGGAG AAATCATCAA GCGCTTCGAA CAGAAAGGCT TCAAGTTAGT GGCTATGAAG 
TTAATCCAGG CAGATGAGGA TCTTCTTAGA CAGCACTACA GTGACCTGAA GGATCGGCCT 
TTTTTCCCTG GACTTGTCAG TTATATGTCT GCTGGTCCTG TGGTTGCAAT GGTGTGGGAA 
GGTTTTAATG TTATAAAAAC TGGCAGAGTA ATGCTTGGCG AGACTAATCC CATTGACTCC 
AAGCCTGGGA CTATCCGAGG TGACTTTTGC GTTCAAGTTG GACGGAACAT TATTCACGGC 
AGTGATTCTG TGGAAAGTGC CAACACTGAG ATCAACCTGT GGTTCAAACC AGATGAGATC 
TGCGATTACA CCAAATGTCA GGAAAGCTGG ATCAACAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nme2a ( XM_021480031.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335706.1 Danio rerio NME/NM23 nucleoside diphosphate kinase 2a (nme2a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa148141
Clone ID Related Accession (Same CDS sequence)	XM_021480031.1
Accession Version	XM_021480031.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	nucleoside diphosphate kinase B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCAG GGGTTTCATC ACCCAGACCC CCCACCACAC CTGCAGCCCA GCCGGACAAA 
TGTACAACCG CTCCAATGTC TGGGAATGAA GAGCGCACTT TCATCGCCAT CAAGCCGGAT 
GGGGTGCAGA GAGGACTCGT CGGAGAAATC ATCAAGCGCT TCGAACAGAA AGGCTTCAAG 
TTAGTGGCTA TGAAGTTAAT CCAGGCAGAT GAGGATCTTC TTAGACAGCA CTACAGTGAC 
CTGAAGGATC GGCCTTTTTT CCCTGGACTT GTCAGTTATA TGTCTGCTGG TCCTGTGGTT 
GCAATGGTGT GGGAAGGTTT TAATGTTATA AAAACTGGCA GAGTAATGCT TGGCGAGACT 
AATCCCATTG ACTCCAAGCC TGGGACTATC CGAGGTGACT TTTGCGTTCA AGTTGGACGG 
AACATTATTC ACGGCAGTGA TTCTGTGGAA AGTGCCAACA CTGAGATCAA CCTGTGGTTC 
AAACCAGATG AGATCTGCGA TTACACCAAA TGTCAGGAAA GCTGGATCAA CAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335706.1
CDS	83..619
Translation	MGSGVSSPRPPTTPAAQPDKCTTAPMSGNEERTFIAIKPDGVQRGLVGEIIKRFEQKGFKLVAMKLIQADEDLLRQHYSDLKDRPFFPGLVSYMSAGPVVAMVWEGFNVIKTGRVMLGETNPIDSKPGTIRGDFCVQVGRNIIHGSDSVESANTEINLWFKPDEICDYTKCQESWINS

Target ORF information:

RefSeq Version	XM_021480031.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NME/NM23 nucleoside diphosphate kinase 2a (nme2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480031.1

ORF Insert Sequence:

ATGGGCTCAG GGGTTTCATC ACCCAGACCC CCCACCACAC CTGCAGCCCA GCCGGACAAA 
TGTACAACCG CTCCAATGTC TGGGAATGAA GAGCGCACTT TCATCGCCAT CAAGCCGGAT 
GGGGTGCAGA GAGGACTCGT CGGAGAAATC ATCAAGCGCT TCGAACAGAA AGGCTTCAAG 
TTAGTGGCTA TGAAGTTAAT CCAGGCAGAT GAGGATCTTC TTAGACAGCA CTACAGTGAC 
CTGAAGGATC GGCCTTTTTT CCCTGGACTT GTCAGTTATA TGTCTGCTGG TCCTGTGGTT 
GCAATGGTGT GGGAAGGTTT TAATGTTATA AAAACTGGCA GAGTAATGCT TGGCGAGACT 
AATCCCATTG ACTCCAAGCC TGGGACTATC CGAGGTGACT TTTGCGTTCA AGTTGGACGG 
AACATTATTC ACGGCAGTGA TTCTGTGGAA AGTGCCAACA CTGAGATCAA CCTGTGGTTC 
AAACCAGATG AGATCTGCGA TTACACCAAA TGTCAGGAAA GCTGGATCAA CAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nme2a ( XM_005160367.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160424.1 Danio rerio NME/NM23 nucleoside diphosphate kinase 2a (nme2a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa17841
Clone ID Related Accession (Same CDS sequence)	XM_005160367.1
Accession Version	XM_005160367.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	nucleoside diphosphate kinase B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCGTGG ATTACAGAAA GCGCGTGGGG GGAGGAATCA ACTTCAGACT CTTGGACTGC 
CATCAAACAA TTCCAATGTC TGGGAATGAA GAGCGCACTT TCATCGCCAT CAAGCCGGAT 
GGGGTGCAGA GAGGACTCGT CGGAGAAATC ATCAAGCGCT TCGAACAGAA AGGCTTCAAG 
TTAGTGGCTA TGAAGTTAAT CCAGGCAGAT GAGGATCTTC TTAGACAGCA CTACAGTGAC 
CTGAAGGATC GGCCTTTTTT CCCTGGACTT GTCAGTTATA TGTCTGCTGG TCCTGTGGTT 
GCAATGGTGT GGGAAGGTTT TAATGTTATA AAAACTGGCA GAGTAATGCT TGGCGAGACT 
AATCCCATTG ACTCCAAGCC TGGGACTATC CGAGGTGACT TTTGCGTTCA AGTTGGACGG 
AACATTATTC ACGGCAGTGA TTCTGTGGAA AGTGCCAACA CTGAGATCAA CCTGTGGTTC 
AAACCAGATG AGATCTGCGA TTACACCAAA TGTCAGGAAA GCTGGATCAA CAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160424.1
CDS	4..540
Translation	MIVDYRKRVGGGINFRLLDCHQTIPMSGNEERTFIAIKPDGVQRGLVGEIIKRFEQKGFKLVAMKLIQADEDLLRQHYSDLKDRPFFPGLVSYMSAGPVVAMVWEGFNVIKTGRVMLGETNPIDSKPGTIRGDFCVQVGRNIIHGSDSVESANTEINLWFKPDEICDYTKCQESWINS

Target ORF information:

RefSeq Version	XM_005160367.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NME/NM23 nucleoside diphosphate kinase 2a (nme2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160367.1

ORF Insert Sequence:

ATGATCGTGG ATTACAGAAA GCGCGTGGGG GGAGGAATCA ACTTCAGACT CTTGGACTGC 
CATCAAACAA TTCCAATGTC TGGGAATGAA GAGCGCACTT TCATCGCCAT CAAGCCGGAT 
GGGGTGCAGA GAGGACTCGT CGGAGAAATC ATCAAGCGCT TCGAACAGAA AGGCTTCAAG 
TTAGTGGCTA TGAAGTTAAT CCAGGCAGAT GAGGATCTTC TTAGACAGCA CTACAGTGAC 
CTGAAGGATC GGCCTTTTTT CCCTGGACTT GTCAGTTATA TGTCTGCTGG TCCTGTGGTT 
GCAATGGTGT GGGAAGGTTT TAATGTTATA AAAACTGGCA GAGTAATGCT TGGCGAGACT 
AATCCCATTG ACTCCAAGCC TGGGACTATC CGAGGTGACT TTTGCGTTCA AGTTGGACGG 
AACATTATTC ACGGCAGTGA TTCTGTGGAA AGTGCCAACA CTGAGATCAA CCTGTGGTTC 
AAACCAGATG AGATCTGCGA TTACACCAAA TGTCAGGAAA GCTGGATCAA CAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Nucleoside diphosphate kinase B regulates angiogenesis through modulation of vascular endothelial growth factor receptor type 2 and endothelial adherens junction proteins.
Arteriosclerosis, thrombosis, and vascular biology34(10)2292-300(2014 Oct)
Feng Y,Gross S,Wolf NM,Butenschön VM,Qiu Y,Devraj K,Liebner S,Kroll J,Skolnik EY,Hammes HP,Wieland T

Irx7, a Smarca4-regulated gene for retinal differentiation, regulates other genes controlled by Smarca4 in zebrafish retinas.
Gene expression patterns : GEP13(5-6)177-82(2013 Jun-Jul)
Zhang Y,Bonilla S,Chong L,Leung YF

Cellular expression of Smarca4 (Brg1)-regulated genes in zebrafish retinas.
BMC developmental biology1145(2011 Jul)
Hensley MR,Emran F,Bonilla S,Zhang L,Zhong W,Grosu P,Dowling JE,Leung YF

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nme2a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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