usp19 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	usp19
Entrez Gene ID	324335
Full Name	ubiquitin specific peptidase 19
Synonyms	si:dkey-32e6.9,wu:fc26a05
General protein information	Preferred Names ubiquitin specific peptidase 19 Names ubiquitin carboxyl-terminal hydrolase 19
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005162119.3	XP_005162176.1	ubiquitin carboxyl-terminal hydrolase 19 isoform X2
XM_005162120.4	XP_005162177.1	ubiquitin carboxyl-terminal hydrolase 19 isoform X3
XM_021469937.1	XP_021325612.1	ubiquitin carboxyl-terminal hydrolase 19 isoform X4
XM_005162118.4	XP_005162175.1	ubiquitin carboxyl-terminal hydrolase 19 isoform X1
XM_005162120.3	XP_005162177.1	ubiquitin carboxyl-terminal hydrolase 19 isoform X3
XM_005162118.3	XP_005162175.1	ubiquitin carboxyl-terminal hydrolase 19 isoform X1
XM_005162119.4	XP_005162176.1	ubiquitin carboxyl-terminal hydrolase 19 isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Usp19	NP_082080.3
Bos taurus (cattle)	USP19	XP_005223068.1
Homo sapiens (human)	USP19	NP_001186089.1
Pan troglodytes (chimpanzee)	USP19	XP_003309901.1
Canis lupus familiaris (dog)	USP19	XP_005632614.1
Rattus norvegicus (Norway rat)	Usp19	NP_001001516.1
Caenorhabditis elegans (roundworm)	F07A11.4	NP_001254305.1
Gallus gallus (chicken)	USP19	XP_004944685.1
Macaca mulatta (Rhesus monkey)	USP19	XP_002802845.1
Danio rerio (zebrafish)	usp19	XP_005162175.1
Xenopus tropicalis (tropical clawed frog)	usp19	NP_001072879.1

Gene Ontology

The following usp19 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the usp19 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa151551	XM_021469937.1 Latest version!	Danio rerio ubiquitin specific peptidase 19 (usp19), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa27651	XM_005162118.4 Latest version!	Danio rerio ubiquitin specific peptidase 19 (usp19), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa27652	XM_005162119.4 Latest version!	Danio rerio ubiquitin specific peptidase 19 (usp19), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa27653	XM_005162120.4 Latest version!	Danio rerio ubiquitin specific peptidase 19 (usp19), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
View Old Accession Versions >>
ODa27653	XM_005162120.3	Danio rerio ubiquitin specific peptidase 19 (usp19), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa27651	XM_005162118.3	Danio rerio ubiquitin specific peptidase 19 (usp19), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa27652	XM_005162119.3	Danio rerio ubiquitin specific peptidase 19 (usp19), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

usp19 ( XM_021469937.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325612.1 Danio rerio ubiquitin specific peptidase 19 (usp19), transcript variant X4, mRNA.

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CloneID	ODa151551
Clone ID Related Accession (Same CDS sequence)	XM_021469937.1
Accession Version	XM_021469937.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 19 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCAG ACTTGTTTCT GGACTGGAAG CAGAATGCAT ACGAGGTGAT AGTGAGGCTG 
AACTGTGGAG GTGCTGGGGA GCTGAGAGTA GAAGATATCG AGTATGACTT TTCAAATTCC 
GCCTGCCACA TCCGGCTGCC AGATGGACGT GAATGGAGCT GTCATCTCCA TGAAGAGATT 
GAAGCCTCCT GCAGTAAATT GAATTATAAG GAGAAATCGA ATGTCCTGCA GCTTGTCATG 
CACAAGAAGA TCCCGCTCAA CACCTGGCCC GCCTACGCTA AAAAGTCAGA ACCAGTGAAC 
ATTCTTCAAA AAAATGGAAG CAACAACTAC CAGTCAAGCA CAGGACGGTC TGAAAAATCT 
AGCGAAGCCA TTGAGAAAAC GCCCCCAGTG AGCTCTGCTG AGCAGAAACG AGGTAAACCT 
GACCGAGGGC TGAAACGAGG GATGAAAGGC AAGCAGGTGC ACGTCACTAA GAGCACAGGA 
GAAAAAACGC CAGAGATTAA ATCAAGCGAT AAAGCAGACT CGATCAATGA GCCCAGTGCA 
AAGCGCACCA CGCGGCCCTC TAAAAACACA AAGGAGCCCC CCCTAGCTCC CTGCTTGGGA 
AACGAGTCGA AGTCCAAAGC AACAGTTAAT GGGAAGACGC ACACATCAGA GGTTAACAGC 
GGTGCCTCTT CATCAAGCAA TGCAAAAGAC AACAGGGCTC AGATGCAGGG CAAACCTCAA 
GGATGCCAGG CATTGCAAAA AGAAAAAGGG ATTGACAGGA AGCCAAAGAG CAATGCTCAG 
GTTGGATCAG GGGAAAGCAA GAGCGTCTCT GTGTGTGAGC CGCAGGTACC GCAAAAAGAC 
CATTCTACTC CTGTAAAAAC ATGCACTGAT AAAATAGCGT CTGATGAGCC AGAAAACAGA 
AAGGAGGAAA CGAAGCCACA GGCTGAAGGC CACATCCCTA AAATGCTTCC CTCTCATGAC 
TTGGGATCTG GAGAGCCTGC ATCAACAGAC TCCAAAAGAG AGGAGTCTCC ACAAAAGAGC 
CACACAGAGG AGAAGAGGGA CAAGTCCAAA GAGGAGCCAC AAGGAGCAAG CCAAGAGGAG 
GATACGGAAG GTCCGGAGCC AATGGTGGAC TTGAAGTTTG TGAAGAATGA CTCGTATGAG 
AAGGGAACAG ATCTGATGGT TGTGAATGTC TACATGAAAG AAATCTGCCG GCAGACGTCC 
AGGGTGCTGT TCAGGGAACA AGACTTCACT CTTATCTTCC AGACCAGTGA TCCCAACTTT 
TTGCGCCTTC ATTCGGATTG CGGACCAAAC ACTGTCTTTA AATGGCAGGT TAAACTCAGG 
AATCTAATTC AGCCCGATCA GTCCAACTAC GCCTTCACTC CGTCCCGTAT CGACATCACC 
CTGAAGAAGA GACACAGTCA GCGCTGGGGG GGTCTGGAGG CCCCTGCCAC ACAAGGTGCA 
GTGGGGGGCG CCAAGGTTGC TGTGGCCTCC AGCCCTTCTA CACTTGAAAA GAGTCAGCCA 
GGAAGCAGCC AGCATGCACT TCCTGCAAAA GAAGAACCAC GGGTTGGAGA GGAGAAACCC 
AAACCCCCTA GAGCCCCAGA GGACTCTGGT TTGGATGCTG TGTCCCCACG CTCAGTTTCC 
GAACATGTGT CCCTCAAACA AGAACCTGCT GTTGCCACGC CCAAACCCAC CTGCATGGTG 
CAGCCCATGA CCCACACTCC TCCGGTAGGC AATGAGAGAT CAGAAGAGGT GGAGGAGAAA 
AAAGTATGTC ACCCTGGCTT CACTGGACTG GTCAATCTGG GAAACACTTG CTTTATGAAC 
AGTGTGATCC AGTCCCTCTC CAACACACGG GAGCTCAGGG ATTATTTCCA TGATCGAGGT 
TTTGAGTCAG AGATTAATTG TAATAACCCA TTGGGCACGG GTGGACGGCT GGCCATTGGC 
TTTGCTGTTC TGCTACGGGC GCTGTGGAAG GGCACTCATC ATGCTTTTCA ACCCTCCAAA 
TTAAAGGCTA TCGTTGCCAG TAAAGCCAGT CAGTTTACAG GTTATGCGCA GCATGATGCT 
CAGGAGTTTA TGGCTTTTTT GCTGGATGGT CTTCATGAAG ACCTGAACCG CATCCAAAAC 
AAACCATACA CCGAGACTGT GGACTCAGAC GGCCGTCAGG ATGAAGTTGT TGCAGAAGAG 
GCATGGCAGA GGCATAAAAT GAGAAACGAT TCCTTCATTG TTGACCTCTT TCAAGGCCAG 
TACAAGTCAA AGCTGGTGTG TCCAATGTGC TCCAAGGTTT CCATAACCTT TGATCCATTC 
TTGTACTTGC CAGTCCCTCT ACCTCAGAAG CAGAAGGTGC TGACTGTTTT CTATTTTGCT 
AAAGAGCCTC ACAAGAAACC AGTCAAGTTC TTGGTTAGTG TGAGTAAAGA GAGCTCAAGC 
ACGGCTGAAG TACTGGAGTC AATATCTCGC AGCGTGAGGA TCAAACCAGA GAACCTGAGG 
CTGACAGAGG TTGTGAAGGG CAGGTTCCAC AGAATCTTCT CACCGTCTCA ATCTTTAGAC 
ACGGTCTCGT CCAGTGACCT GCTCTTCTGC TTTGAGATGC TGTCAAAAGA TCTCATCAAA 
GAGAGAGTGG TGTTGCTGAA GGTCCAGCAG AGACCTCAGA TTCCTAGCAT ACCAATCACA 
AAGTGCGCTG GTTGTCTGAA GCCTCCTGCC TCTGACGATG AGAAGCTGAA GCGCTGCACA 
CGCTGTTATC GTGTCGGCTA CTGCAATCAG ACCTGCCAGA AGAACCACTG GCCTTCTCAC 
AAGACTATGT GTCGGTCTAA TGTGGAAAAC ATTGGACAGC CATTTTTAAT CAGCGTGCCT 
GAGTCTAGAC TTTCTTACAC TCGCCTCACA CAGCTTCTAG AAGGCTACTC TAGGTACTCT 
GTCAATGTGT TCCAGCCACC TTTCCAGTCA GGTCGGACGT CCCCTGAGTC CAGTCTGTCC 
CGTGTTGACC TGACTTCCAT GGCGAGCAGC GTCCCAGAGT GTCAAGAGAA GGACCTGCCA 
TCAGCAGGTG ACCCAGAAAA CCCAGCAGAG CAGGATGAAT CTGTCACAGC CTCACAAACG 
GAGAGTGCAG CAGATGGAGG AGACACTCTC TCCACACATA CCACTGACTC TGGTTGCTGT 
GAGCTCGCCT CCAGCTCCCA TGATTCTCTG CTGGAGAAAG AAACGTCATG TGAAAAGGCT 
GTTAAACCAG AAGCTGTAGT AACTGGGTAT CAACAGCCTT CTGAGTCGGC GAGTGGAGGT 
GCATCTCAAT TTTACATCAC TATTCTGGAC TCGAGCCAAA AGGAAGACAA AAAACTTGAA 
GAGAAAGGTG ACGCGGTACT GGAGCTGCCT GATGAGTGCA CACTAGAGCT GGTGTGGAAG 
AACAACGAGC GGCAGAAGGA ATATGTGCTG GTGCGCTCTA AAGATCTGGA GTTTGATGAG 
GACCCGGGCT CTGCTACCGA GACCTCCAGG GCAGGACACT TCACCTTAGA GCAGTGCCTC 
AACCTCTTTA CCAAACCTGA GGTCCTAGCC CCTGAGGAGG CATGGTACTG TCCTAAGTGT 
CAGCAGCACA GAGAGGCCTC TAAACAGCTC CTACTTTGGC GTCTTCCGAA TGTACTCATC 
ATCCAGCTCA AGCGCTTCTC ATTTAGGAGT TTCATATGGA GGGACAAGAT TAATGACATG 
GTCGATTTTC CAGTCAGAAA CTTGGACTTG AGTAAGTTTT GTATTGGTCA TAAGGGTGAC 
ATCCAGCAGC CGCCAATCTA TGATCTGTAC GCTGTCATCA ACCATTATGG TGGAATGATT 
GGAGGACACT ACACAGCCTA TGCTCGCCTT CCCAGCGACA AGAACAGCCA GCTTAGTGAT 
GTTGGCTGGC GTTTGTTTGA TGACAGCACA GTCACGACGG TGGAGGAAAG TCAGGTGGTG 
ACGCGTTACG CCTATGTGCT GTTCTATCGA CGCAGGAACT CTCCAGTGGA AAGGCCGTCT 
CACCTGCTGG GACCCCTTGG CGCTGAATCG TCTGCTGCCA CAGGTGGTGC TGCCAGTCAG 
GCGTCTCTGA TATGGCAGGA ACTGGAGGAG GATGAGGAGG GGCAGCGAGA GGCCTCTTTT 
CGGAGCCTGT TCCGCCCCGG TCTGCGCGGA AGAAGAGCCA GGATGAGACC TGAGCAAGAA 
GAAGAAGAGG AGGGCAGGGG AGGGCTTTCT CACCGCAGAC GAGATGACAA CTCAGATTAT 
TCTGATGGCG AACGCATGAG ATATTTCATC TTGGGCACTG TGGCGGCAAT CCTAGCATTG 
CTGTTCAATC TCATCTATCC TTTGTTGTAC TGGTCCAACT GGGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325612.1
CDS	141..4448
Translation	MKSDLFLDWKQNAYEVIVRLNCGGAGELRVEDIEYDFSNSACHIRLPDGREWSCHLHEEIEASCSKLNYKEKSNVLQLVMHKKIPLNTWPAYAKKSEPVNILQKNGSNNYQSSTGRSEKSSEAIEKTPPVSSAEQKRGKPDRGLKRGMKGKQVHVTKSTGEKTPEIKSSDKADSINEPSAKRTTRPSKNTKEPPLAPCLGNESKSKATVNGKTHTSEVNSGASSSSNAKDNRAQMQGKPQGCQALQKEKGIDRKPKSNAQVGSGESKSVSVCEPQVPQKDHSTPVKTCTDKIASDEPENRKEETKPQAEGHIPKMLPSHDLGSGEPASTDSKREESPQKSHTEEKRDKSKEEPQGASQEEDTEGPEPMVDLKFVKNDSYEKGTDLMVVNVYMKEICRQTSRVLFREQDFTLIFQTSDPNFLRLHSDCGPNTVFKWQVKLRNLIQPDQSNYAFTPSRIDITLKKRHSQRWGGLEAPATQGAVGGAKVAVASSPSTLEKSQPGSSQHALPAKEEPRVGEEKPKPPRAPEDSGLDAVSPRSVSEHVSLKQEPAVATPKPTCMVQPMTHTPPVGNERSEEVEEKKVCHPGFTGLVNLGNTCFMNSVIQSLSNTRELRDYFHDRGFESEINCNNPLGTGGRLAIGFAVLLRALWKGTHHAFQPSKLKAIVASKASQFTGYAQHDAQEFMAFLLDGLHEDLNRIQNKPYTETVDSDGRQDEVVAEEAWQRHKMRNDSFIVDLFQGQYKSKLVCPMCSKVSITFDPFLYLPVPLPQKQKVLTVFYFAKEPHKKPVKFLVSVSKESSSTAEVLESISRSVRIKPENLRLTEVVKGRFHRIFSPSQSLDTVSSSDLLFCFEMLSKDLIKERVVLLKVQQRPQIPSIPITKCAGCLKPPASDDEKLKRCTRCYRVGYCNQTCQKNHWPSHKTMCRSNVENIGQPFLISVPESRLSYTRLTQLLEGYSRYSVNVFQPPFQSGRTSPESSLSRVDLTSMASSVPECQEKDLPSAGDPENPAEQDESVTASQTESAADGGDTLSTHTTDSGCCELASSSHDSLLEKETSCEKAVKPEAVVTGYQQPSESASGGASQFYITILDSSQKEDKKLEEKGDAVLELPDECTLELVWKNNERQKEYVLVRSKDLEFDEDPGSATETSRAGHFTLEQCLNLFTKPEVLAPEEAWYCPKCQQHREASKQLLLWRLPNVLIIQLKRFSFRSFIWRDKINDMVDFPVRNLDLSKFCIGHKGDIQQPPIYDLYAVINHYGGMIGGHYTAYARLPSDKNSQLSDVGWRLFDDSTVTTVEESQVVTRYAYVLFYRRRNSPVERPSHLLGPLGAESSAATGGAASQASLIWQELEEDEEGQREASFRSLFRPGLRGRRARMRPEQEEEEEGRGGLSHRRRDDNSDYSDGERMRYFILGTVAAILALLFNLIYPLLYWSNWV

Target ORF information:

RefSeq Version	XM_021469937.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 19 (usp19), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469937.1

ORF Insert Sequence:

ATGAAATCAG ACTTGTTTCT GGACTGGAAG CAGAATGCAT ACGAGGTGAT AGTGAGGCTG 
AACTGTGGAG GTGCTGGGGA GCTGAGAGTA GAAGATATCG AGTATGACTT TTCAAATTCC 
GCCTGCCACA TCCGGCTGCC AGATGGACGT GAATGGAGCT GTCATCTCCA TGAAGAGATT 
GAAGCCTCCT GCAGTAAATT GAATTATAAG GAGAAATCGA ATGTCCTGCA GCTTGTCATG 
CACAAGAAGA TCCCGCTCAA CACCTGGCCC GCCTACGCTA AAAAGTCAGA ACCAGTGAAC 
ATTCTTCAAA AAAATGGAAG CAACAACTAC CAGTCAAGCA CAGGACGGTC TGAAAAATCT 
AGCGAAGCCA TTGAGAAAAC GCCCCCAGTG AGCTCTGCTG AGCAGAAACG AGGTAAACCT 
GACCGAGGGC TGAAACGAGG GATGAAAGGC AAGCAGGTGC ACGTCACTAA GAGCACAGGA 
GAAAAAACGC CAGAGATTAA ATCAAGCGAT AAAGCAGACT CGATCAATGA GCCCAGTGCA 
AAGCGCACCA CGCGGCCCTC TAAAAACACA AAGGAGCCCC CCCTAGCTCC CTGCTTGGGA 
AACGAGTCGA AGTCCAAAGC AACAGTTAAT GGGAAGACGC ACACATCAGA GGTTAACAGC 
GGTGCCTCTT CATCAAGCAA TGCAAAAGAC AACAGGGCTC AGATGCAGGG CAAACCTCAA 
GGATGCCAGG CATTGCAAAA AGAAAAAGGG ATTGACAGGA AGCCAAAGAG CAATGCTCAG 
GTTGGATCAG GGGAAAGCAA GAGCGTCTCT GTGTGTGAGC CGCAGGTACC GCAAAAAGAC 
CATTCTACTC CTGTAAAAAC ATGCACTGAT AAAATAGCGT CTGATGAGCC AGAAAACAGA 
AAGGAGGAAA CGAAGCCACA GGCTGAAGGC CACATCCCTA AAATGCTTCC CTCTCATGAC 
TTGGGATCTG GAGAGCCTGC ATCAACAGAC TCCAAAAGAG AGGAGTCTCC ACAAAAGAGC 
CACACAGAGG AGAAGAGGGA CAAGTCCAAA GAGGAGCCAC AAGGAGCAAG CCAAGAGGAG 
GATACGGAAG GTCCGGAGCC AATGGTGGAC TTGAAGTTTG TGAAGAATGA CTCGTATGAG 
AAGGGAACAG ATCTGATGGT TGTGAATGTC TACATGAAAG AAATCTGCCG GCAGACGTCC 
AGGGTGCTGT TCAGGGAACA AGACTTCACT CTTATCTTCC AGACCAGTGA TCCCAACTTT 
TTGCGCCTTC ATTCGGATTG CGGACCAAAC ACTGTCTTTA AATGGCAGGT TAAACTCAGG 
AATCTAATTC AGCCCGATCA GTCCAACTAC GCCTTCACTC CGTCCCGTAT CGACATCACC 
CTGAAGAAGA GACACAGTCA GCGCTGGGGG GGTCTGGAGG CCCCTGCCAC ACAAGGTGCA 
GTGGGGGGCG CCAAGGTTGC TGTGGCCTCC AGCCCTTCTA CACTTGAAAA GAGTCAGCCA 
GGAAGCAGCC AGCATGCACT TCCTGCAAAA GAAGAACCAC GGGTTGGAGA GGAGAAACCC 
AAACCCCCTA GAGCCCCAGA GGACTCTGGT TTGGATGCTG TGTCCCCACG CTCAGTTTCC 
GAACATGTGT CCCTCAAACA AGAACCTGCT GTTGCCACGC CCAAACCCAC CTGCATGGTG 
CAGCCCATGA CCCACACTCC TCCGGTAGGC AATGAGAGAT CAGAAGAGGT GGAGGAGAAA 
AAAGTATGTC ACCCTGGCTT CACTGGACTG GTCAATCTGG GAAACACTTG CTTTATGAAC 
AGTGTGATCC AGTCCCTCTC CAACACACGG GAGCTCAGGG ATTATTTCCA TGATCGAGGT 
TTTGAGTCAG AGATTAATTG TAATAACCCA TTGGGCACGG GTGGACGGCT GGCCATTGGC 
TTTGCTGTTC TGCTACGGGC GCTGTGGAAG GGCACTCATC ATGCTTTTCA ACCCTCCAAA 
TTAAAGGCTA TCGTTGCCAG TAAAGCCAGT CAGTTTACAG GTTATGCGCA GCATGATGCT 
CAGGAGTTTA TGGCTTTTTT GCTGGATGGT CTTCATGAAG ACCTGAACCG CATCCAAAAC 
AAACCATACA CCGAGACTGT GGACTCAGAC GGCCGTCAGG ATGAAGTTGT TGCAGAAGAG 
GCATGGCAGA GGCATAAAAT GAGAAACGAT TCCTTCATTG TTGACCTCTT TCAAGGCCAG 
TACAAGTCAA AGCTGGTGTG TCCAATGTGC TCCAAGGTTT CCATAACCTT TGATCCATTC 
TTGTACTTGC CAGTCCCTCT ACCTCAGAAG CAGAAGGTGC TGACTGTTTT CTATTTTGCT 
AAAGAGCCTC ACAAGAAACC AGTCAAGTTC TTGGTTAGTG TGAGTAAAGA GAGCTCAAGC 
ACGGCTGAAG TACTGGAGTC AATATCTCGC AGCGTGAGGA TCAAACCAGA GAACCTGAGG 
CTGACAGAGG TTGTGAAGGG CAGGTTCCAC AGAATCTTCT CACCGTCTCA ATCTTTAGAC 
ACGGTCTCGT CCAGTGACCT GCTCTTCTGC TTTGAGATGC TGTCAAAAGA TCTCATCAAA 
GAGAGAGTGG TGTTGCTGAA GGTCCAGCAG AGACCTCAGA TTCCTAGCAT ACCAATCACA 
AAGTGCGCTG GTTGTCTGAA GCCTCCTGCC TCTGACGATG AGAAGCTGAA GCGCTGCACA 
CGCTGTTATC GTGTCGGCTA CTGCAATCAG ACCTGCCAGA AGAACCACTG GCCTTCTCAC 
AAGACTATGT GTCGGTCTAA TGTGGAAAAC ATTGGACAGC CATTTTTAAT CAGCGTGCCT 
GAGTCTAGAC TTTCTTACAC TCGCCTCACA CAGCTTCTAG AAGGCTACTC TAGGTACTCT 
GTCAATGTGT TCCAGCCACC TTTCCAGTCA GGTCGGACGT CCCCTGAGTC CAGTCTGTCC 
CGTGTTGACC TGACTTCCAT GGCGAGCAGC GTCCCAGAGT GTCAAGAGAA GGACCTGCCA 
TCAGCAGGTG ACCCAGAAAA CCCAGCAGAG CAGGATGAAT CTGTCACAGC CTCACAAACG 
GAGAGTGCAG CAGATGGAGG AGACACTCTC TCCACACATA CCACTGACTC TGGTTGCTGT 
GAGCTCGCCT CCAGCTCCCA TGATTCTCTG CTGGAGAAAG AAACGTCATG TGAAAAGGCT 
GTTAAACCAG AAGCTGTAGT AACTGGGTAT CAACAGCCTT CTGAGTCGGC GAGTGGAGGT 
GCATCTCAAT TTTACATCAC TATTCTGGAC TCGAGCCAAA AGGAAGACAA AAAACTTGAA 
GAGAAAGGTG ACGCGGTACT GGAGCTGCCT GATGAGTGCA CACTAGAGCT GGTGTGGAAG 
AACAACGAGC GGCAGAAGGA ATATGTGCTG GTGCGCTCTA AAGATCTGGA GTTTGATGAG 
GACCCGGGCT CTGCTACCGA GACCTCCAGG GCAGGACACT TCACCTTAGA GCAGTGCCTC 
AACCTCTTTA CCAAACCTGA GGTCCTAGCC CCTGAGGAGG CATGGTACTG TCCTAAGTGT 
CAGCAGCACA GAGAGGCCTC TAAACAGCTC CTACTTTGGC GTCTTCCGAA TGTACTCATC 
ATCCAGCTCA AGCGCTTCTC ATTTAGGAGT TTCATATGGA GGGACAAGAT TAATGACATG 
GTCGATTTTC CAGTCAGAAA CTTGGACTTG AGTAAGTTTT GTATTGGTCA TAAGGGTGAC 
ATCCAGCAGC CGCCAATCTA TGATCTGTAC GCTGTCATCA ACCATTATGG TGGAATGATT 
GGAGGACACT ACACAGCCTA TGCTCGCCTT CCCAGCGACA AGAACAGCCA GCTTAGTGAT 
GTTGGCTGGC GTTTGTTTGA TGACAGCACA GTCACGACGG TGGAGGAAAG TCAGGTGGTG 
ACGCGTTACG CCTATGTGCT GTTCTATCGA CGCAGGAACT CTCCAGTGGA AAGGCCGTCT 
CACCTGCTGG GACCCCTTGG CGCTGAATCG TCTGCTGCCA CAGGTGGTGC TGCCAGTCAG 
GCGTCTCTGA TATGGCAGGA ACTGGAGGAG GATGAGGAGG GGCAGCGAGA GGCCTCTTTT 
CGGAGCCTGT TCCGCCCCGG TCTGCGCGGA AGAAGAGCCA GGATGAGACC TGAGCAAGAA 
GAAGAAGAGG AGGGCAGGGG AGGGCTTTCT CACCGCAGAC GAGATGACAA CTCAGATTAT 
TCTGATGGCG AACGCATGAG ATATTTCATC TTGGGCACTG TGGCGGCAAT CCTAGCATTG 
CTGTTCAATC TCATCTATCC TTTGTTGTAC TGGTCCAACT GGGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp19 ( XM_005162118.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162175.1 Danio rerio ubiquitin specific peptidase 19 (usp19), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa27651
Clone ID Related Accession (Same CDS sequence)	XM_005162118.4 , XM_005162118.3
Accession Version	XM_005162118.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 19 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162118.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCA GCAGCACAGG ATCCAGCGAA TCAGGCCGCA GGCGAGGTCA GAGGGGACCG 
GATGACACGG TCAGCACCAG CAAGAAGAAG CAGAAGGACA GAGCCAACCA GGAGAGCAAA 
GAGGCCAAAC GGGTGGCTAC CAGCACAGAG ACTAAGAAAG ACTTGTTTCT GGACTGGAAG 
CAGAATGCAT ACGAGGTGAT AGTGAGGCTG AACTGTGGAG GTGCTGGGGA GCTGAGAGTA 
GAAGATATCG AGTATGACTT TTCAAATTCC GCCTGCCACA TCCGGCTGCC AGATGGACGT 
GAATGGAGCT GTCATCTCCA TGAAGAGATT GAAGCCTCCT GCAGTAAATT GAATTATAAG 
GAGAAATCGA ATGTCCTGCA GCTTGTCATG CACAAGAAGA TCCCGCTCAA CACCTGGCCC 
GCCTACGCTA AAAAGTCAGA ACCAGTGAAC ATTCTTCAAA AAAATGGAAG CAACAACTAC 
CAGTCAAGCA CAGGACGGTC TGAAAAATCT AGCGAAGCCA TTGAGAAAAC GCCCCCAGTG 
AGCTCTGCTG AGCAGAAACG AGGTAAACCT GACCGAGGGC TGAAACGAGG GATGAAAGGC 
AAGCAGGTGC ACGTCACTAA GAGCACAGGA GAAAAAACGC CAGAGATTAA ATCAAGCGAT 
AAAGCAGACT CGATCAATGA GCCCAGTGCA AAGCGCACCA CGCGGCCCTC TAAAAACACA 
AAGGAGCCCC CCCTAGCTCC CTGCTTGGGA AACGAGTCGA AGTCCAAAGC AACAGTTAAT 
GGGAAGACGC ACACATCAGA GGTTAACAGC GGTGCCTCTT CATCAAGCAA TGCAAAAGAC 
AACAGGGCTC AGATGCAGGG CAAACCTCAA GGATGCCAGG CATTGCAAAA AGAAAAAGGG 
ATTGACAGGA AGCCAAAGAG CAATGCTCAG GTTGGATCAG GGGAAAGCAA GAGCGTCTCT 
GTGTGTGAGC CGCAGGTACC GCAAAAAGAC CATTCTACTC CTGTAAAAAC ATGCACTGAT 
AAAATAGCGT CTGATGAGCC AGAAAACAGA AAGGAGGAAA CGAAGCCACA GGCTGAAGGC 
CACATCCCTA AAATGCTTCC CTCTCATGAC TTGGGATCTG GAGAGCCTGC ATCAACAGAC 
TCCAAAAGAG AGGAGTCTCC ACAAAAGAGC CACACAGAGG AGAAGAGGGA CAAGTCCAAA 
GAGGAGCCAC AAGGAGCAAG CCAAGAGGAG GATACGGAAG GTCCGGAGCC AATGGTGGAC 
TTGAAGTTTG TGAAGAATGA CTCGTATGAG AAGGGAACAG ATCTGATGGT TGTGAATGTC 
TACATGAAAG AAATCTGCCG GCAGACGTCC AGGGTGCTGT TCAGGGAACA AGACTTCACT 
CTTATCTTCC AGACCAGTGA TCCCAACTTT TTGCGCCTTC ATTCGGATTG CGGACCAAAC 
ACTGTCTTTA AATGGCAGGT TAAACTCAGG AATCTAATTC AGCCCGATCA GTCCAACTAC 
GCCTTCACTC CGTCCCGTAT CGACATCACC CTGAAGAAGA GACACAGTCA GCGCTGGGGG 
GGTCTGGAGG CCCCTGCCAC ACAAGGTGCA GTGGGGGGCG CCAAGGTTGC TGTGGCCTCC 
AGCCCTTCTA CACTTGAAAA GAGTCAGCCA GGAAGCAGCC AGCATGCACT TCCTGCAAAA 
GAAGAACCAC GGGTTGGAGA GGAGAAACCC AAACCCCCTA GAGCCCCAGA GGACTCTGGT 
TTGGATGCTG TGTCCCCACG CTCAGTTTCC GAACATGTGT CCCTCAAACA AGAACCTGCT 
GTTGCCACGC CCAAACCCAC CTGCATGGTG CAGCCCATGA CCCACACTCC TCCGGTAGGC 
AATGAGAGAT CAGAAGAGGT GGAGGAGAAA AAAGTATGTC ACCCTGGCTT CACTGGACTG 
GTCAATCTGG GAAACACTTG CTTTATGAAC AGTGTGATCC AGTCCCTCTC CAACACACGG 
GAGCTCAGGG ATTATTTCCA TGATCGAGGT TTTGAGTCAG AGATTAATTG TAATAACCCA 
TTGGGCACGG GTGGACGGCT GGCCATTGGC TTTGCTGTTC TGCTACGGGC GCTGTGGAAG 
GGCACTCATC ATGCTTTTCA ACCCTCCAAA TTAAAGGCTA TCGTTGCCAG TAAAGCCAGT 
CAGTTTACAG GTTATGCGCA GCATGATGCT CAGGAGTTTA TGGCTTTTTT GCTGGATGGT 
CTTCATGAAG ACCTGAACCG CATCCAAAAC AAACCATACA CCGAGACTGT GGACTCAGAC 
GGCCGTCAGG ATGAAGTTGT TGCAGAAGAG GCATGGCAGA GGCATAAAAT GAGAAACGAT 
TCCTTCATTG TTGACCTCTT TCAAGGCCAG TACAAGTCAA AGCTGGTGTG TCCAATGTGC 
TCCAAGGTTT CCATAACCTT TGATCCATTC TTGTACTTGC CAGTCCCTCT ACCTCAGAAG 
CAGAAGGTGC TGACTGTTTT CTATTTTGCT AAAGAGCCTC ACAAGAAACC AGTCAAGTTC 
TTGGTTAGTG TGAGTAAAGA GAGCTCAAGC ACGGCTGAAG TACTGGAGTC AATATCTCGC 
AGCGTGAGGA TCAAACCAGA GAACCTGAGG CTGACAGAGG TTGTGAAGGG CAGGTTCCAC 
AGAATCTTCT CACCGTCTCA ATCTTTAGAC ACGGTCTCGT CCAGTGACCT GCTCTTCTGC 
TTTGAGATGC TGTCAAAAGA TCTCATCAAA GAGAGAGTGG TGTTGCTGAA GGTCCAGCAG 
AGACCTCAGA TTCCTAGCAT ACCAATCACA AAGTGCGCTG GTTGTCTGAA GCCTCCTGCC 
TCTGACGATG AGAAGCTGAA GCGCTGCACA CGCTGTTATC GTGTCGGCTA CTGCAATCAG 
ACCTGCCAGA AGAACCACTG GCCTTCTCAC AAGACTATGT GTCGGTCTAA TGTGGAAAAC 
ATTGGACAGC CATTTTTAAT CAGCGTGCCT GAGTCTAGAC TTTCTTACAC TCGCCTCACA 
CAGCTTCTAG AAGGCTACTC TAGGTACTCT GTCAATGTGT TCCAGCCACC TTTCCAGTCA 
GGTCGGACGT CCCCTGAGTC CAGTCTGTCC CGTGTTGACC TGACTTCCAT GGCGAGCAGC 
GTCCCAGAGT GTCAAGAGAA GGACCTGCCA TCAGCAGGTG ACCCAGAAAA CCCAGCAGAG 
CAGGATGAAT CTGTCACAGC CTCACAAACG GAGAGTGCAG CAGATGGAGG AGACACTCTC 
TCCACACATA CCACTGACTC TGGTTGCTGT GAGCTCGCCT CCAGCTCCCA TGATTCTCTG 
CTGGAGAAAG AAACGTCATG TGAAAAGGCT GTTAAACCAG AAGCTGTAGT AACTGGGTAT 
CAACAGCCTT CTGAGTCGGC GAGTGGAGGT GCATCTCAAT TTTACATCAC TATTCTGGAC 
TCGAGCCAAA AGGAAGACAA AAAACTTGAA GAGAAAGGTG ACGCGGTACT GGAGCTGCCT 
GATGAGTGCA CACTAGAGCT GGTGTGGAAG AACAACGAGC GGCAGAAGGA ATATGTGCTG 
GTGCGCTCTA AAGATCTGGA GTTTGATGAG GACCCGGGCT CTGCTACCGA GACCTCCAGG 
GCAGGACACT TCACCTTAGA GCAGTGCCTC AACCTCTTTA CCAAACCTGA GGTCCTAGCC 
CCTGAGGAGG CATGGTACTG TCCTAAGTGT CAGCAGCACA GAGAGGCCTC TAAACAGCTC 
CTACTTTGGC GTCTTCCGAA TGTACTCATC ATCCAGCTCA AGCGCTTCTC ATTTAGGAGT 
TTCATATGGA GGGACAAGAT TAATGACATG GTCGATTTTC CAGTCAGAAA CTTGGACTTG 
AGTAAGTTTT GTATTGGTCA TAAGGGTGAC ATCCAGCAGC CGCCAATCTA TGATCTGTAC 
GCTGTCATCA ACCATTATGG TGGAATGATT GGAGGACACT ACACAGCCTA TGCTCGCCTT 
CCCAGCGACA AGAACAGCCA GCTTAGTGAT GTTGGCTGGC GTTTGTTTGA TGACAGCACA 
GTCACGACGG TGGAGGAAAG TCAGGTGGTG ACGCGTTACG CCTATGTGCT GTTCTATCGA 
CGCAGGAACT CTCCAGTGGA AAGGCCGTCT CACCTGCTGG GACCCCTTGG CGCTGAATCG 
TCTGCTGCCA CAGGTGGTGC TGCCAGTCAG GCGTCTCTGA TATGGCAGGA ACTGGAGGAG 
GATGAGGAGG GGCAGCGAGA GGCCTCTTTT CGGAGCCTGT TCCGCCCCGG TCTGCGCGGA 
AGAAGAGCCA GGATGAGACC TGAGCAAGAA GAAGAAGAGG AGGGCAGGGG AGGGCTTTCT 
CACCGCAGAC GAGATGACAA CTCAGATTAT TCTGATGGCG AACGCATGAG ATATTTCATC 
TTGGGCACTG TGGCGGCAAT CCTAGCATTG CTGTTCAATC TCATCTATCC TTTGTTGTAC 
TGGTCCAACT GGGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162175.1
CDS	540..4997
Translation	MASSSTGSSESGRRRGQRGPDDTVSTSKKKQKDRANQESKEAKRVATSTETKKDLFLDWKQNAYEVIVRLNCGGAGELRVEDIEYDFSNSACHIRLPDGREWSCHLHEEIEASCSKLNYKEKSNVLQLVMHKKIPLNTWPAYAKKSEPVNILQKNGSNNYQSSTGRSEKSSEAIEKTPPVSSAEQKRGKPDRGLKRGMKGKQVHVTKSTGEKTPEIKSSDKADSINEPSAKRTTRPSKNTKEPPLAPCLGNESKSKATVNGKTHTSEVNSGASSSSNAKDNRAQMQGKPQGCQALQKEKGIDRKPKSNAQVGSGESKSVSVCEPQVPQKDHSTPVKTCTDKIASDEPENRKEETKPQAEGHIPKMLPSHDLGSGEPASTDSKREESPQKSHTEEKRDKSKEEPQGASQEEDTEGPEPMVDLKFVKNDSYEKGTDLMVVNVYMKEICRQTSRVLFREQDFTLIFQTSDPNFLRLHSDCGPNTVFKWQVKLRNLIQPDQSNYAFTPSRIDITLKKRHSQRWGGLEAPATQGAVGGAKVAVASSPSTLEKSQPGSSQHALPAKEEPRVGEEKPKPPRAPEDSGLDAVSPRSVSEHVSLKQEPAVATPKPTCMVQPMTHTPPVGNERSEEVEEKKVCHPGFTGLVNLGNTCFMNSVIQSLSNTRELRDYFHDRGFESEINCNNPLGTGGRLAIGFAVLLRALWKGTHHAFQPSKLKAIVASKASQFTGYAQHDAQEFMAFLLDGLHEDLNRIQNKPYTETVDSDGRQDEVVAEEAWQRHKMRNDSFIVDLFQGQYKSKLVCPMCSKVSITFDPFLYLPVPLPQKQKVLTVFYFAKEPHKKPVKFLVSVSKESSSTAEVLESISRSVRIKPENLRLTEVVKGRFHRIFSPSQSLDTVSSSDLLFCFEMLSKDLIKERVVLLKVQQRPQIPSIPITKCAGCLKPPASDDEKLKRCTRCYRVGYCNQTCQKNHWPSHKTMCRSNVENIGQPFLISVPESRLSYTRLTQLLEGYSRYSVNVFQPPFQSGRTSPESSLSRVDLTSMASSVPECQEKDLPSAGDPENPAEQDESVTASQTESAADGGDTLSTHTTDSGCCELASSSHDSLLEKETSCEKAVKPEAVVTGYQQPSESASGGASQFYITILDSSQKEDKKLEEKGDAVLELPDECTLELVWKNNERQKEYVLVRSKDLEFDEDPGSATETSRAGHFTLEQCLNLFTKPEVLAPEEAWYCPKCQQHREASKQLLLWRLPNVLIIQLKRFSFRSFIWRDKINDMVDFPVRNLDLSKFCIGHKGDIQQPPIYDLYAVINHYGGMIGGHYTAYARLPSDKNSQLSDVGWRLFDDSTVTTVEESQVVTRYAYVLFYRRRNSPVERPSHLLGPLGAESSAATGGAASQASLIWQELEEDEEGQREASFRSLFRPGLRGRRARMRPEQEEEEEGRGGLSHRRRDDNSDYSDGERMRYFILGTVAAILALLFNLIYPLLYWSNWV

Target ORF information:

RefSeq Version	XM_005162118.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 19 (usp19), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162118.4

ORF Insert Sequence:

ATGGCCAGCA GCAGCACAGG ATCCAGCGAA TCAGGCCGCA GGCGAGGTCA GAGGGGACCG 
GATGACACGG TCAGCACCAG CAAGAAGAAG CAGAAGGACA GAGCCAACCA GGAGAGCAAA 
GAGGCCAAAC GGGTGGCTAC CAGCACAGAG ACTAAGAAAG ACTTGTTTCT GGACTGGAAG 
CAGAATGCAT ACGAGGTGAT AGTGAGGCTG AACTGTGGAG GTGCTGGGGA GCTGAGAGTA 
GAAGATATCG AGTATGACTT TTCAAATTCC GCCTGCCACA TCCGGCTGCC AGATGGACGT 
GAATGGAGCT GTCATCTCCA TGAAGAGATT GAAGCCTCCT GCAGTAAATT GAATTATAAG 
GAGAAATCGA ATGTCCTGCA GCTTGTCATG CACAAGAAGA TCCCGCTCAA CACCTGGCCC 
GCCTACGCTA AAAAGTCAGA ACCAGTGAAC ATTCTTCAAA AAAATGGAAG CAACAACTAC 
CAGTCAAGCA CAGGACGGTC TGAAAAATCT AGCGAAGCCA TTGAGAAAAC GCCCCCAGTG 
AGCTCTGCTG AGCAGAAACG AGGTAAACCT GACCGAGGGC TGAAACGAGG GATGAAAGGC 
AAGCAGGTGC ACGTCACTAA GAGCACAGGA GAAAAAACGC CAGAGATTAA ATCAAGCGAT 
AAAGCAGACT CGATCAATGA GCCCAGTGCA AAGCGCACCA CGCGGCCCTC TAAAAACACA 
AAGGAGCCCC CCCTAGCTCC CTGCTTGGGA AACGAGTCGA AGTCCAAAGC AACAGTTAAT 
GGGAAGACGC ACACATCAGA GGTTAACAGC GGTGCCTCTT CATCAAGCAA TGCAAAAGAC 
AACAGGGCTC AGATGCAGGG CAAACCTCAA GGATGCCAGG CATTGCAAAA AGAAAAAGGG 
ATTGACAGGA AGCCAAAGAG CAATGCTCAG GTTGGATCAG GGGAAAGCAA GAGCGTCTCT 
GTGTGTGAGC CGCAGGTACC GCAAAAAGAC CATTCTACTC CTGTAAAAAC ATGCACTGAT 
AAAATAGCGT CTGATGAGCC AGAAAACAGA AAGGAGGAAA CGAAGCCACA GGCTGAAGGC 
CACATCCCTA AAATGCTTCC CTCTCATGAC TTGGGATCTG GAGAGCCTGC ATCAACAGAC 
TCCAAAAGAG AGGAGTCTCC ACAAAAGAGC CACACAGAGG AGAAGAGGGA CAAGTCCAAA 
GAGGAGCCAC AAGGAGCAAG CCAAGAGGAG GATACGGAAG GTCCGGAGCC AATGGTGGAC 
TTGAAGTTTG TGAAGAATGA CTCGTATGAG AAGGGAACAG ATCTGATGGT TGTGAATGTC 
TACATGAAAG AAATCTGCCG GCAGACGTCC AGGGTGCTGT TCAGGGAACA AGACTTCACT 
CTTATCTTCC AGACCAGTGA TCCCAACTTT TTGCGCCTTC ATTCGGATTG CGGACCAAAC 
ACTGTCTTTA AATGGCAGGT TAAACTCAGG AATCTAATTC AGCCCGATCA GTCCAACTAC 
GCCTTCACTC CGTCCCGTAT CGACATCACC CTGAAGAAGA GACACAGTCA GCGCTGGGGG 
GGTCTGGAGG CCCCTGCCAC ACAAGGTGCA GTGGGGGGCG CCAAGGTTGC TGTGGCCTCC 
AGCCCTTCTA CACTTGAAAA GAGTCAGCCA GGAAGCAGCC AGCATGCACT TCCTGCAAAA 
GAAGAACCAC GGGTTGGAGA GGAGAAACCC AAACCCCCTA GAGCCCCAGA GGACTCTGGT 
TTGGATGCTG TGTCCCCACG CTCAGTTTCC GAACATGTGT CCCTCAAACA AGAACCTGCT 
GTTGCCACGC CCAAACCCAC CTGCATGGTG CAGCCCATGA CCCACACTCC TCCGGTAGGC 
AATGAGAGAT CAGAAGAGGT GGAGGAGAAA AAAGTATGTC ACCCTGGCTT CACTGGACTG 
GTCAATCTGG GAAACACTTG CTTTATGAAC AGTGTGATCC AGTCCCTCTC CAACACACGG 
GAGCTCAGGG ATTATTTCCA TGATCGAGGT TTTGAGTCAG AGATTAATTG TAATAACCCA 
TTGGGCACGG GTGGACGGCT GGCCATTGGC TTTGCTGTTC TGCTACGGGC GCTGTGGAAG 
GGCACTCATC ATGCTTTTCA ACCCTCCAAA TTAAAGGCTA TCGTTGCCAG TAAAGCCAGT 
CAGTTTACAG GTTATGCGCA GCATGATGCT CAGGAGTTTA TGGCTTTTTT GCTGGATGGT 
CTTCATGAAG ACCTGAACCG CATCCAAAAC AAACCATACA CCGAGACTGT GGACTCAGAC 
GGCCGTCAGG ATGAAGTTGT TGCAGAAGAG GCATGGCAGA GGCATAAAAT GAGAAACGAT 
TCCTTCATTG TTGACCTCTT TCAAGGCCAG TACAAGTCAA AGCTGGTGTG TCCAATGTGC 
TCCAAGGTTT CCATAACCTT TGATCCATTC TTGTACTTGC CAGTCCCTCT ACCTCAGAAG 
CAGAAGGTGC TGACTGTTTT CTATTTTGCT AAAGAGCCTC ACAAGAAACC AGTCAAGTTC 
TTGGTTAGTG TGAGTAAAGA GAGCTCAAGC ACGGCTGAAG TACTGGAGTC AATATCTCGC 
AGCGTGAGGA TCAAACCAGA GAACCTGAGG CTGACAGAGG TTGTGAAGGG CAGGTTCCAC 
AGAATCTTCT CACCGTCTCA ATCTTTAGAC ACGGTCTCGT CCAGTGACCT GCTCTTCTGC 
TTTGAGATGC TGTCAAAAGA TCTCATCAAA GAGAGAGTGG TGTTGCTGAA GGTCCAGCAG 
AGACCTCAGA TTCCTAGCAT ACCAATCACA AAGTGCGCTG GTTGTCTGAA GCCTCCTGCC 
TCTGACGATG AGAAGCTGAA GCGCTGCACA CGCTGTTATC GTGTCGGCTA CTGCAATCAG 
ACCTGCCAGA AGAACCACTG GCCTTCTCAC AAGACTATGT GTCGGTCTAA TGTGGAAAAC 
ATTGGACAGC CATTTTTAAT CAGCGTGCCT GAGTCTAGAC TTTCTTACAC TCGCCTCACA 
CAGCTTCTAG AAGGCTACTC TAGGTACTCT GTCAATGTGT TCCAGCCACC TTTCCAGTCA 
GGTCGGACGT CCCCTGAGTC CAGTCTGTCC CGTGTTGACC TGACTTCCAT GGCGAGCAGC 
GTCCCAGAGT GTCAAGAGAA GGACCTGCCA TCAGCAGGTG ACCCAGAAAA CCCAGCAGAG 
CAGGATGAAT CTGTCACAGC CTCACAAACG GAGAGTGCAG CAGATGGAGG AGACACTCTC 
TCCACACATA CCACTGACTC TGGTTGCTGT GAGCTCGCCT CCAGCTCCCA TGATTCTCTG 
CTGGAGAAAG AAACGTCATG TGAAAAGGCT GTTAAACCAG AAGCTGTAGT AACTGGGTAT 
CAACAGCCTT CTGAGTCGGC GAGTGGAGGT GCATCTCAAT TTTACATCAC TATTCTGGAC 
TCGAGCCAAA AGGAAGACAA AAAACTTGAA GAGAAAGGTG ACGCGGTACT GGAGCTGCCT 
GATGAGTGCA CACTAGAGCT GGTGTGGAAG AACAACGAGC GGCAGAAGGA ATATGTGCTG 
GTGCGCTCTA AAGATCTGGA GTTTGATGAG GACCCGGGCT CTGCTACCGA GACCTCCAGG 
GCAGGACACT TCACCTTAGA GCAGTGCCTC AACCTCTTTA CCAAACCTGA GGTCCTAGCC 
CCTGAGGAGG CATGGTACTG TCCTAAGTGT CAGCAGCACA GAGAGGCCTC TAAACAGCTC 
CTACTTTGGC GTCTTCCGAA TGTACTCATC ATCCAGCTCA AGCGCTTCTC ATTTAGGAGT 
TTCATATGGA GGGACAAGAT TAATGACATG GTCGATTTTC CAGTCAGAAA CTTGGACTTG 
AGTAAGTTTT GTATTGGTCA TAAGGGTGAC ATCCAGCAGC CGCCAATCTA TGATCTGTAC 
GCTGTCATCA ACCATTATGG TGGAATGATT GGAGGACACT ACACAGCCTA TGCTCGCCTT 
CCCAGCGACA AGAACAGCCA GCTTAGTGAT GTTGGCTGGC GTTTGTTTGA TGACAGCACA 
GTCACGACGG TGGAGGAAAG TCAGGTGGTG ACGCGTTACG CCTATGTGCT GTTCTATCGA 
CGCAGGAACT CTCCAGTGGA AAGGCCGTCT CACCTGCTGG GACCCCTTGG CGCTGAATCG 
TCTGCTGCCA CAGGTGGTGC TGCCAGTCAG GCGTCTCTGA TATGGCAGGA ACTGGAGGAG 
GATGAGGAGG GGCAGCGAGA GGCCTCTTTT CGGAGCCTGT TCCGCCCCGG TCTGCGCGGA 
AGAAGAGCCA GGATGAGACC TGAGCAAGAA GAAGAAGAGG AGGGCAGGGG AGGGCTTTCT 
CACCGCAGAC GAGATGACAA CTCAGATTAT TCTGATGGCG AACGCATGAG ATATTTCATC 
TTGGGCACTG TGGCGGCAAT CCTAGCATTG CTGTTCAATC TCATCTATCC TTTGTTGTAC 
TGGTCCAACT GGGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp19 ( XM_005162118.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162175.1 Danio rerio ubiquitin specific peptidase 19 (usp19), transcript variant X1, mRNA.

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CloneID	ODa27651
Clone ID Related Accession (Same CDS sequence)	XM_005162118.4 , XM_005162118.3
Accession Version	XM_005162118.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 19 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162118.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCA GCAGCACAGG ATCCAGCGAA TCAGGCCGCA GGCGAGGTCA GAGGGGACCG 
GATGACACGG TCAGCACCAG CAAGAAGAAG CAGAAGGACA GAGCCAACCA GGAGAGCAAA 
GAGGCCAAAC GGGTGGCTAC CAGCACAGAG ACTAAGAAAG ACTTGTTTCT GGACTGGAAG 
CAGAATGCAT ACGAGGTGAT AGTGAGGCTG AACTGTGGAG GTGCTGGGGA GCTGAGAGTA 
GAAGATATCG AGTATGACTT TTCAAATTCC GCCTGCCACA TCCGGCTGCC AGATGGACGT 
GAATGGAGCT GTCATCTCCA TGAAGAGATT GAAGCCTCCT GCAGTAAATT GAATTATAAG 
GAGAAATCGA ATGTCCTGCA GCTTGTCATG CACAAGAAGA TCCCGCTCAA CACCTGGCCC 
GCCTACGCTA AAAAGTCAGA ACCAGTGAAC ATTCTTCAAA AAAATGGAAG CAACAACTAC 
CAGTCAAGCA CAGGACGGTC TGAAAAATCT AGCGAAGCCA TTGAGAAAAC GCCCCCAGTG 
AGCTCTGCTG AGCAGAAACG AGGTAAACCT GACCGAGGGC TGAAACGAGG GATGAAAGGC 
AAGCAGGTGC ACGTCACTAA GAGCACAGGA GAAAAAACGC CAGAGATTAA ATCAAGCGAT 
AAAGCAGACT CGATCAATGA GCCCAGTGCA AAGCGCACCA CGCGGCCCTC TAAAAACACA 
AAGGAGCCCC CCCTAGCTCC CTGCTTGGGA AACGAGTCGA AGTCCAAAGC AACAGTTAAT 
GGGAAGACGC ACACATCAGA GGTTAACAGC GGTGCCTCTT CATCAAGCAA TGCAAAAGAC 
AACAGGGCTC AGATGCAGGG CAAACCTCAA GGATGCCAGG CATTGCAAAA AGAAAAAGGG 
ATTGACAGGA AGCCAAAGAG CAATGCTCAG GTTGGATCAG GGGAAAGCAA GAGCGTCTCT 
GTGTGTGAGC CGCAGGTACC GCAAAAAGAC CATTCTACTC CTGTAAAAAC ATGCACTGAT 
AAAATAGCGT CTGATGAGCC AGAAAACAGA AAGGAGGAAA CGAAGCCACA GGCTGAAGGC 
CACATCCCTA AAATGCTTCC CTCTCATGAC TTGGGATCTG GAGAGCCTGC ATCAACAGAC 
TCCAAAAGAG AGGAGTCTCC ACAAAAGAGC CACACAGAGG AGAAGAGGGA CAAGTCCAAA 
GAGGAGCCAC AAGGAGCAAG CCAAGAGGAG GATACGGAAG GTCCGGAGCC AATGGTGGAC 
TTGAAGTTTG TGAAGAATGA CTCGTATGAG AAGGGAACAG ATCTGATGGT TGTGAATGTC 
TACATGAAAG AAATCTGCCG GCAGACGTCC AGGGTGCTGT TCAGGGAACA AGACTTCACT 
CTTATCTTCC AGACCAGTGA TCCCAACTTT TTGCGCCTTC ATTCGGATTG CGGACCAAAC 
ACTGTCTTTA AATGGCAGGT TAAACTCAGG AATCTAATTC AGCCCGATCA GTCCAACTAC 
GCCTTCACTC CGTCCCGTAT CGACATCACC CTGAAGAAGA GACACAGTCA GCGCTGGGGG 
GGTCTGGAGG CCCCTGCCAC ACAAGGTGCA GTGGGGGGCG CCAAGGTTGC TGTGGCCTCC 
AGCCCTTCTA CACTTGAAAA GAGTCAGCCA GGAAGCAGCC AGCATGCACT TCCTGCAAAA 
GAAGAACCAC GGGTTGGAGA GGAGAAACCC AAACCCCCTA GAGCCCCAGA GGACTCTGGT 
TTGGATGCTG TGTCCCCACG CTCAGTTTCC GAACATGTGT CCCTCAAACA AGAACCTGCT 
GTTGCCACGC CCAAACCCAC CTGCATGGTG CAGCCCATGA CCCACACTCC TCCGGTAGGC 
AATGAGAGAT CAGAAGAGGT GGAGGAGAAA AAAGTATGTC ACCCTGGCTT CACTGGACTG 
GTCAATCTGG GAAACACTTG CTTTATGAAC AGTGTGATCC AGTCCCTCTC CAACACACGG 
GAGCTCAGGG ATTATTTCCA TGATCGAGGT TTTGAGTCAG AGATTAATTG TAATAACCCA 
TTGGGCACGG GTGGACGGCT GGCCATTGGC TTTGCTGTTC TGCTACGGGC GCTGTGGAAG 
GGCACTCATC ATGCTTTTCA ACCCTCCAAA TTAAAGGCTA TCGTTGCCAG TAAAGCCAGT 
CAGTTTACAG GTTATGCGCA GCATGATGCT CAGGAGTTTA TGGCTTTTTT GCTGGATGGT 
CTTCATGAAG ACCTGAACCG CATCCAAAAC AAACCATACA CCGAGACTGT GGACTCAGAC 
GGCCGTCAGG ATGAAGTTGT TGCAGAAGAG GCATGGCAGA GGCATAAAAT GAGAAACGAT 
TCCTTCATTG TTGACCTCTT TCAAGGCCAG TACAAGTCAA AGCTGGTGTG TCCAATGTGC 
TCCAAGGTTT CCATAACCTT TGATCCATTC TTGTACTTGC CAGTCCCTCT ACCTCAGAAG 
CAGAAGGTGC TGACTGTTTT CTATTTTGCT AAAGAGCCTC ACAAGAAACC AGTCAAGTTC 
TTGGTTAGTG TGAGTAAAGA GAGCTCAAGC ACGGCTGAAG TACTGGAGTC AATATCTCGC 
AGCGTGAGGA TCAAACCAGA GAACCTGAGG CTGACAGAGG TTGTGAAGGG CAGGTTCCAC 
AGAATCTTCT CACCGTCTCA ATCTTTAGAC ACGGTCTCGT CCAGTGACCT GCTCTTCTGC 
TTTGAGATGC TGTCAAAAGA TCTCATCAAA GAGAGAGTGG TGTTGCTGAA GGTCCAGCAG 
AGACCTCAGA TTCCTAGCAT ACCAATCACA AAGTGCGCTG GTTGTCTGAA GCCTCCTGCC 
TCTGACGATG AGAAGCTGAA GCGCTGCACA CGCTGTTATC GTGTCGGCTA CTGCAATCAG 
ACCTGCCAGA AGAACCACTG GCCTTCTCAC AAGACTATGT GTCGGTCTAA TGTGGAAAAC 
ATTGGACAGC CATTTTTAAT CAGCGTGCCT GAGTCTAGAC TTTCTTACAC TCGCCTCACA 
CAGCTTCTAG AAGGCTACTC TAGGTACTCT GTCAATGTGT TCCAGCCACC TTTCCAGTCA 
GGTCGGACGT CCCCTGAGTC CAGTCTGTCC CGTGTTGACC TGACTTCCAT GGCGAGCAGC 
GTCCCAGAGT GTCAAGAGAA GGACCTGCCA TCAGCAGGTG ACCCAGAAAA CCCAGCAGAG 
CAGGATGAAT CTGTCACAGC CTCACAAACG GAGAGTGCAG CAGATGGAGG AGACACTCTC 
TCCACACATA CCACTGACTC TGGTTGCTGT GAGCTCGCCT CCAGCTCCCA TGATTCTCTG 
CTGGAGAAAG AAACGTCATG TGAAAAGGCT GTTAAACCAG AAGCTGTAGT AACTGGGTAT 
CAACAGCCTT CTGAGTCGGC GAGTGGAGGT GCATCTCAAT TTTACATCAC TATTCTGGAC 
TCGAGCCAAA AGGAAGACAA AAAACTTGAA GAGAAAGGTG ACGCGGTACT GGAGCTGCCT 
GATGAGTGCA CACTAGAGCT GGTGTGGAAG AACAACGAGC GGCAGAAGGA ATATGTGCTG 
GTGCGCTCTA AAGATCTGGA GTTTGATGAG GACCCGGGCT CTGCTACCGA GACCTCCAGG 
GCAGGACACT TCACCTTAGA GCAGTGCCTC AACCTCTTTA CCAAACCTGA GGTCCTAGCC 
CCTGAGGAGG CATGGTACTG TCCTAAGTGT CAGCAGCACA GAGAGGCCTC TAAACAGCTC 
CTACTTTGGC GTCTTCCGAA TGTACTCATC ATCCAGCTCA AGCGCTTCTC ATTTAGGAGT 
TTCATATGGA GGGACAAGAT TAATGACATG GTCGATTTTC CAGTCAGAAA CTTGGACTTG 
AGTAAGTTTT GTATTGGTCA TAAGGGTGAC ATCCAGCAGC CGCCAATCTA TGATCTGTAC 
GCTGTCATCA ACCATTATGG TGGAATGATT GGAGGACACT ACACAGCCTA TGCTCGCCTT 
CCCAGCGACA AGAACAGCCA GCTTAGTGAT GTTGGCTGGC GTTTGTTTGA TGACAGCACA 
GTCACGACGG TGGAGGAAAG TCAGGTGGTG ACGCGTTACG CCTATGTGCT GTTCTATCGA 
CGCAGGAACT CTCCAGTGGA AAGGCCGTCT CACCTGCTGG GACCCCTTGG CGCTGAATCG 
TCTGCTGCCA CAGGTGGTGC TGCCAGTCAG GCGTCTCTGA TATGGCAGGA ACTGGAGGAG 
GATGAGGAGG GGCAGCGAGA GGCCTCTTTT CGGAGCCTGT TCCGCCCCGG TCTGCGCGGA 
AGAAGAGCCA GGATGAGACC TGAGCAAGAA GAAGAAGAGG AGGGCAGGGG AGGGCTTTCT 
CACCGCAGAC GAGATGACAA CTCAGATTAT TCTGATGGCG AACGCATGAG ATATTTCATC 
TTGGGCACTG TGGCGGCAAT CCTAGCATTG CTGTTCAATC TCATCTATCC TTTGTTGTAC 
TGGTCCAACT GGGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162175.1
CDS	539..4996
Translation	MASSSTGSSESGRRRGQRGPDDTVSTSKKKQKDRANQESKEAKRVATSTETKKDLFLDWKQNAYEVIVRLNCGGAGELRVEDIEYDFSNSACHIRLPDGREWSCHLHEEIEASCSKLNYKEKSNVLQLVMHKKIPLNTWPAYAKKSEPVNILQKNGSNNYQSSTGRSEKSSEAIEKTPPVSSAEQKRGKPDRGLKRGMKGKQVHVTKSTGEKTPEIKSSDKADSINEPSAKRTTRPSKNTKEPPLAPCLGNESKSKATVNGKTHTSEVNSGASSSSNAKDNRAQMQGKPQGCQALQKEKGIDRKPKSNAQVGSGESKSVSVCEPQVPQKDHSTPVKTCTDKIASDEPENRKEETKPQAEGHIPKMLPSHDLGSGEPASTDSKREESPQKSHTEEKRDKSKEEPQGASQEEDTEGPEPMVDLKFVKNDSYEKGTDLMVVNVYMKEICRQTSRVLFREQDFTLIFQTSDPNFLRLHSDCGPNTVFKWQVKLRNLIQPDQSNYAFTPSRIDITLKKRHSQRWGGLEAPATQGAVGGAKVAVASSPSTLEKSQPGSSQHALPAKEEPRVGEEKPKPPRAPEDSGLDAVSPRSVSEHVSLKQEPAVATPKPTCMVQPMTHTPPVGNERSEEVEEKKVCHPGFTGLVNLGNTCFMNSVIQSLSNTRELRDYFHDRGFESEINCNNPLGTGGRLAIGFAVLLRALWKGTHHAFQPSKLKAIVASKASQFTGYAQHDAQEFMAFLLDGLHEDLNRIQNKPYTETVDSDGRQDEVVAEEAWQRHKMRNDSFIVDLFQGQYKSKLVCPMCSKVSITFDPFLYLPVPLPQKQKVLTVFYFAKEPHKKPVKFLVSVSKESSSTAEVLESISRSVRIKPENLRLTEVVKGRFHRIFSPSQSLDTVSSSDLLFCFEMLSKDLIKERVVLLKVQQRPQIPSIPITKCAGCLKPPASDDEKLKRCTRCYRVGYCNQTCQKNHWPSHKTMCRSNVENIGQPFLISVPESRLSYTRLTQLLEGYSRYSVNVFQPPFQSGRTSPESSLSRVDLTSMASSVPECQEKDLPSAGDPENPAEQDESVTASQTESAADGGDTLSTHTTDSGCCELASSSHDSLLEKETSCEKAVKPEAVVTGYQQPSESASGGASQFYITILDSSQKEDKKLEEKGDAVLELPDECTLELVWKNNERQKEYVLVRSKDLEFDEDPGSATETSRAGHFTLEQCLNLFTKPEVLAPEEAWYCPKCQQHREASKQLLLWRLPNVLIIQLKRFSFRSFIWRDKINDMVDFPVRNLDLSKFCIGHKGDIQQPPIYDLYAVINHYGGMIGGHYTAYARLPSDKNSQLSDVGWRLFDDSTVTTVEESQVVTRYAYVLFYRRRNSPVERPSHLLGPLGAESSAATGGAASQASLIWQELEEDEEGQREASFRSLFRPGLRGRRARMRPEQEEEEEGRGGLSHRRRDDNSDYSDGERMRYFILGTVAAILALLFNLIYPLLYWSNWV

Target ORF information:

RefSeq Version	XM_005162118.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 19 (usp19), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162118.3

ORF Insert Sequence:

ATGGCCAGCA GCAGCACAGG ATCCAGCGAA TCAGGCCGCA GGCGAGGTCA GAGGGGACCG 
GATGACACGG TCAGCACCAG CAAGAAGAAG CAGAAGGACA GAGCCAACCA GGAGAGCAAA 
GAGGCCAAAC GGGTGGCTAC CAGCACAGAG ACTAAGAAAG ACTTGTTTCT GGACTGGAAG 
CAGAATGCAT ACGAGGTGAT AGTGAGGCTG AACTGTGGAG GTGCTGGGGA GCTGAGAGTA 
GAAGATATCG AGTATGACTT TTCAAATTCC GCCTGCCACA TCCGGCTGCC AGATGGACGT 
GAATGGAGCT GTCATCTCCA TGAAGAGATT GAAGCCTCCT GCAGTAAATT GAATTATAAG 
GAGAAATCGA ATGTCCTGCA GCTTGTCATG CACAAGAAGA TCCCGCTCAA CACCTGGCCC 
GCCTACGCTA AAAAGTCAGA ACCAGTGAAC ATTCTTCAAA AAAATGGAAG CAACAACTAC 
CAGTCAAGCA CAGGACGGTC TGAAAAATCT AGCGAAGCCA TTGAGAAAAC GCCCCCAGTG 
AGCTCTGCTG AGCAGAAACG AGGTAAACCT GACCGAGGGC TGAAACGAGG GATGAAAGGC 
AAGCAGGTGC ACGTCACTAA GAGCACAGGA GAAAAAACGC CAGAGATTAA ATCAAGCGAT 
AAAGCAGACT CGATCAATGA GCCCAGTGCA AAGCGCACCA CGCGGCCCTC TAAAAACACA 
AAGGAGCCCC CCCTAGCTCC CTGCTTGGGA AACGAGTCGA AGTCCAAAGC AACAGTTAAT 
GGGAAGACGC ACACATCAGA GGTTAACAGC GGTGCCTCTT CATCAAGCAA TGCAAAAGAC 
AACAGGGCTC AGATGCAGGG CAAACCTCAA GGATGCCAGG CATTGCAAAA AGAAAAAGGG 
ATTGACAGGA AGCCAAAGAG CAATGCTCAG GTTGGATCAG GGGAAAGCAA GAGCGTCTCT 
GTGTGTGAGC CGCAGGTACC GCAAAAAGAC CATTCTACTC CTGTAAAAAC ATGCACTGAT 
AAAATAGCGT CTGATGAGCC AGAAAACAGA AAGGAGGAAA CGAAGCCACA GGCTGAAGGC 
CACATCCCTA AAATGCTTCC CTCTCATGAC TTGGGATCTG GAGAGCCTGC ATCAACAGAC 
TCCAAAAGAG AGGAGTCTCC ACAAAAGAGC CACACAGAGG AGAAGAGGGA CAAGTCCAAA 
GAGGAGCCAC AAGGAGCAAG CCAAGAGGAG GATACGGAAG GTCCGGAGCC AATGGTGGAC 
TTGAAGTTTG TGAAGAATGA CTCGTATGAG AAGGGAACAG ATCTGATGGT TGTGAATGTC 
TACATGAAAG AAATCTGCCG GCAGACGTCC AGGGTGCTGT TCAGGGAACA AGACTTCACT 
CTTATCTTCC AGACCAGTGA TCCCAACTTT TTGCGCCTTC ATTCGGATTG CGGACCAAAC 
ACTGTCTTTA AATGGCAGGT TAAACTCAGG AATCTAATTC AGCCCGATCA GTCCAACTAC 
GCCTTCACTC CGTCCCGTAT CGACATCACC CTGAAGAAGA GACACAGTCA GCGCTGGGGG 
GGTCTGGAGG CCCCTGCCAC ACAAGGTGCA GTGGGGGGCG CCAAGGTTGC TGTGGCCTCC 
AGCCCTTCTA CACTTGAAAA GAGTCAGCCA GGAAGCAGCC AGCATGCACT TCCTGCAAAA 
GAAGAACCAC GGGTTGGAGA GGAGAAACCC AAACCCCCTA GAGCCCCAGA GGACTCTGGT 
TTGGATGCTG TGTCCCCACG CTCAGTTTCC GAACATGTGT CCCTCAAACA AGAACCTGCT 
GTTGCCACGC CCAAACCCAC CTGCATGGTG CAGCCCATGA CCCACACTCC TCCGGTAGGC 
AATGAGAGAT CAGAAGAGGT GGAGGAGAAA AAAGTATGTC ACCCTGGCTT CACTGGACTG 
GTCAATCTGG GAAACACTTG CTTTATGAAC AGTGTGATCC AGTCCCTCTC CAACACACGG 
GAGCTCAGGG ATTATTTCCA TGATCGAGGT TTTGAGTCAG AGATTAATTG TAATAACCCA 
TTGGGCACGG GTGGACGGCT GGCCATTGGC TTTGCTGTTC TGCTACGGGC GCTGTGGAAG 
GGCACTCATC ATGCTTTTCA ACCCTCCAAA TTAAAGGCTA TCGTTGCCAG TAAAGCCAGT 
CAGTTTACAG GTTATGCGCA GCATGATGCT CAGGAGTTTA TGGCTTTTTT GCTGGATGGT 
CTTCATGAAG ACCTGAACCG CATCCAAAAC AAACCATACA CCGAGACTGT GGACTCAGAC 
GGCCGTCAGG ATGAAGTTGT TGCAGAAGAG GCATGGCAGA GGCATAAAAT GAGAAACGAT 
TCCTTCATTG TTGACCTCTT TCAAGGCCAG TACAAGTCAA AGCTGGTGTG TCCAATGTGC 
TCCAAGGTTT CCATAACCTT TGATCCATTC TTGTACTTGC CAGTCCCTCT ACCTCAGAAG 
CAGAAGGTGC TGACTGTTTT CTATTTTGCT AAAGAGCCTC ACAAGAAACC AGTCAAGTTC 
TTGGTTAGTG TGAGTAAAGA GAGCTCAAGC ACGGCTGAAG TACTGGAGTC AATATCTCGC 
AGCGTGAGGA TCAAACCAGA GAACCTGAGG CTGACAGAGG TTGTGAAGGG CAGGTTCCAC 
AGAATCTTCT CACCGTCTCA ATCTTTAGAC ACGGTCTCGT CCAGTGACCT GCTCTTCTGC 
TTTGAGATGC TGTCAAAAGA TCTCATCAAA GAGAGAGTGG TGTTGCTGAA GGTCCAGCAG 
AGACCTCAGA TTCCTAGCAT ACCAATCACA AAGTGCGCTG GTTGTCTGAA GCCTCCTGCC 
TCTGACGATG AGAAGCTGAA GCGCTGCACA CGCTGTTATC GTGTCGGCTA CTGCAATCAG 
ACCTGCCAGA AGAACCACTG GCCTTCTCAC AAGACTATGT GTCGGTCTAA TGTGGAAAAC 
ATTGGACAGC CATTTTTAAT CAGCGTGCCT GAGTCTAGAC TTTCTTACAC TCGCCTCACA 
CAGCTTCTAG AAGGCTACTC TAGGTACTCT GTCAATGTGT TCCAGCCACC TTTCCAGTCA 
GGTCGGACGT CCCCTGAGTC CAGTCTGTCC CGTGTTGACC TGACTTCCAT GGCGAGCAGC 
GTCCCAGAGT GTCAAGAGAA GGACCTGCCA TCAGCAGGTG ACCCAGAAAA CCCAGCAGAG 
CAGGATGAAT CTGTCACAGC CTCACAAACG GAGAGTGCAG CAGATGGAGG AGACACTCTC 
TCCACACATA CCACTGACTC TGGTTGCTGT GAGCTCGCCT CCAGCTCCCA TGATTCTCTG 
CTGGAGAAAG AAACGTCATG TGAAAAGGCT GTTAAACCAG AAGCTGTAGT AACTGGGTAT 
CAACAGCCTT CTGAGTCGGC GAGTGGAGGT GCATCTCAAT TTTACATCAC TATTCTGGAC 
TCGAGCCAAA AGGAAGACAA AAAACTTGAA GAGAAAGGTG ACGCGGTACT GGAGCTGCCT 
GATGAGTGCA CACTAGAGCT GGTGTGGAAG AACAACGAGC GGCAGAAGGA ATATGTGCTG 
GTGCGCTCTA AAGATCTGGA GTTTGATGAG GACCCGGGCT CTGCTACCGA GACCTCCAGG 
GCAGGACACT TCACCTTAGA GCAGTGCCTC AACCTCTTTA CCAAACCTGA GGTCCTAGCC 
CCTGAGGAGG CATGGTACTG TCCTAAGTGT CAGCAGCACA GAGAGGCCTC TAAACAGCTC 
CTACTTTGGC GTCTTCCGAA TGTACTCATC ATCCAGCTCA AGCGCTTCTC ATTTAGGAGT 
TTCATATGGA GGGACAAGAT TAATGACATG GTCGATTTTC CAGTCAGAAA CTTGGACTTG 
AGTAAGTTTT GTATTGGTCA TAAGGGTGAC ATCCAGCAGC CGCCAATCTA TGATCTGTAC 
GCTGTCATCA ACCATTATGG TGGAATGATT GGAGGACACT ACACAGCCTA TGCTCGCCTT 
CCCAGCGACA AGAACAGCCA GCTTAGTGAT GTTGGCTGGC GTTTGTTTGA TGACAGCACA 
GTCACGACGG TGGAGGAAAG TCAGGTGGTG ACGCGTTACG CCTATGTGCT GTTCTATCGA 
CGCAGGAACT CTCCAGTGGA AAGGCCGTCT CACCTGCTGG GACCCCTTGG CGCTGAATCG 
TCTGCTGCCA CAGGTGGTGC TGCCAGTCAG GCGTCTCTGA TATGGCAGGA ACTGGAGGAG 
GATGAGGAGG GGCAGCGAGA GGCCTCTTTT CGGAGCCTGT TCCGCCCCGG TCTGCGCGGA 
AGAAGAGCCA GGATGAGACC TGAGCAAGAA GAAGAAGAGG AGGGCAGGGG AGGGCTTTCT 
CACCGCAGAC GAGATGACAA CTCAGATTAT TCTGATGGCG AACGCATGAG ATATTTCATC 
TTGGGCACTG TGGCGGCAAT CCTAGCATTG CTGTTCAATC TCATCTATCC TTTGTTGTAC 
TGGTCCAACT GGGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp19 ( XM_005162119.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162176.1 Danio rerio ubiquitin specific peptidase 19 (usp19), transcript variant X2, mRNA.

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CloneID	ODa27652
Clone ID Related Accession (Same CDS sequence)	XM_005162119.3 , XM_005162119.4
Accession Version	XM_005162119.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4452bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 19 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162119.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCA GCAGCACAGG ATCCAGCGAA TCAGGCCGCA GGCGAGGTCA GAGGGGACCG 
GATGACACGG TCAGCACCAG CAAGAAGAAG CAGAAGGACA GAGCCAACCA GGAGAGCAAA 
GAGGCCAAAC GGGTGGCTAC CAGCACAGAG ACTAAGAAAG ACTTGTTTCT GGACTGGAAG 
CAGAATGCAT ACGAGGTGAT AGTGAGGCTG AACTGTGGAG GTGCTGGGGA GCTGAGAGTA 
GAAGATATCG AGTATGACTT TTCAAATTCC GCCTGCCACA TCCGGCTGCC AGATGGACGT 
GAATGGAGCT GTCATCTCCA TGAAGAGATT GAAGCCTCCT GCAGTAAATT GAATTATAAG 
GAGAAATCGA ATGTCCTGCA GCTTGTCATG CACAAGAAGA TCCCGCTCAA CACCTGGCCC 
GCCTACGCTA AAAAGTCAGA ACCAGTGAAC ATTCTTCAAA AAAATGGAAG CAACAACTAC 
CAGTCAAGCA CAGGACGGTC TGAAAAATCT AGCGAAGCCA TTGAGAAAAC GCCCCCAGTG 
AGCTCTGCTG AGCAGAAACG AGGTAAACCT GACCGAGGGC TGAAACGAGG GATGAAAGGC 
AAGCAGGTGC ACGTCACTAA GAGCACAGGA GAAAAAACGC CAGAGATTAA ATCAAGCGAT 
AAAGCAGACT CGATCAATGA GCCCAGTGCA AAGCGCACCA CGCGGCCCTC TAAAAACACA 
AAGGAGCCCC CCCTAGCTCC CTGCTTGGGA AACGAGTCGA AGTCCAAAGC AACAGTTAAT 
GGGAAGACGC ACACATCAGA GGTTAACAGC GGTGCCTCTT CATCAAGCAA TGCAAAAGAC 
AACAGGGCTC AGATGCAGGG CAAACCTCAA GGATGCCAGG CATTGCAAAA AGAAAAAGGG 
ATTGACAGGA AGCCAAAGAG CAATGCTCAG GTTGGATCAG GGGAAAGCAA GAGCGTCTCT 
GTGTGTGAGC CGCAGGTACC GCAAAAAGAC CATTCTACTC CTGTAAAAAC ATGCACTGAT 
AAAATAGCGT CTGATGAGCC AGAAAACAGA AAGGAGGAAA CGAAGCCACA GGCTGAAGGC 
CACATCCCTA AAATGCTTCC CTCTCATGAC TTGGGATCTG GAGAGCCTGC ATCAACAGAC 
TCCAAAAGAG AGGAGTCTCC ACAAAAGAGC CACACAGAGG AGAAGAGGGA CAAGTCCAAA 
GAGGAGCCAC AAGGAGCAAG CCAAGAGGAG GATACGGAAG GTCCGGAGCC AATGGTGGAC 
TTGAAGTTTG TGAAGAATGA CTCGTATGAG AAGGGAACAG ATCTGATGGT TGTGAATGTC 
TACATGAAAG AAATCTGCCG GCAGACGTCC AGGGTGCTGT TCAGGGAACA AGACTTCACT 
CTTATCTTCC AGACCAGTGA TCCCAACTTT TTGCGCCTTC ATTCGGATTG CGGACCAAAC 
ACTGTCTTTA AATGGCAGGT TAAACTCAGG AATCTAATTC AGCCCGATCA GTCCAACTAC 
GCCTTCACTC CGTCCCGTAT CGACATCACC CTGAAGAAGA GACACAGTCA GCGCTGGGGG 
GGTCTGGAGG CCCCTGCCAC ACAAGTGGGG GGCGCCAAGG TTGCTGTGGC CTCCAGCCCT 
TCTACACTTG AAAAGAGTCA GCCAGGAAGC AGCCAGCATG CACTTCCTGC AAAAGAAGAA 
CCACGGGTTG GAGAGGAGAA ACCCAAACCC CCTAGAGCCC CAGAGGACTC TGGTTTGGAT 
GCTGTGTCCC CACGCTCAGT TTCCGAACAT GTGTCCCTCA AACAAGAACC TGCTGTTGCC 
ACGCCCAAAC CCACCTGCAT GGTGCAGCCC ATGACCCACA CTCCTCCGGT AGGCAATGAG 
AGATCAGAAG AGGTGGAGGA GAAAAAAGTA TGTCACCCTG GCTTCACTGG ACTGGTCAAT 
CTGGGAAACA CTTGCTTTAT GAACAGTGTG ATCCAGTCCC TCTCCAACAC ACGGGAGCTC 
AGGGATTATT TCCATGATCG AGGTTTTGAG TCAGAGATTA ATTGTAATAA CCCATTGGGC 
ACGGGTGGAC GGCTGGCCAT TGGCTTTGCT GTTCTGCTAC GGGCGCTGTG GAAGGGCACT 
CATCATGCTT TTCAACCCTC CAAATTAAAG GCTATCGTTG CCAGTAAAGC CAGTCAGTTT 
ACAGGTTATG CGCAGCATGA TGCTCAGGAG TTTATGGCTT TTTTGCTGGA TGGTCTTCAT 
GAAGACCTGA ACCGCATCCA AAACAAACCA TACACCGAGA CTGTGGACTC AGACGGCCGT 
CAGGATGAAG TTGTTGCAGA AGAGGCATGG CAGAGGCATA AAATGAGAAA CGATTCCTTC 
ATTGTTGACC TCTTTCAAGG CCAGTACAAG TCAAAGCTGG TGTGTCCAAT GTGCTCCAAG 
GTTTCCATAA CCTTTGATCC ATTCTTGTAC TTGCCAGTCC CTCTACCTCA GAAGCAGAAG 
GTGCTGACTG TTTTCTATTT TGCTAAAGAG CCTCACAAGA AACCAGTCAA GTTCTTGGTT 
AGTGTGAGTA AAGAGAGCTC AAGCACGGCT GAAGTACTGG AGTCAATATC TCGCAGCGTG 
AGGATCAAAC CAGAGAACCT GAGGCTGACA GAGGTTGTGA AGGGCAGGTT CCACAGAATC 
TTCTCACCGT CTCAATCTTT AGACACGGTC TCGTCCAGTG ACCTGCTCTT CTGCTTTGAG 
ATGCTGTCAA AAGATCTCAT CAAAGAGAGA GTGGTGTTGC TGAAGGTCCA GCAGAGACCT 
CAGATTCCTA GCATACCAAT CACAAAGTGC GCTGGTTGTC TGAAGCCTCC TGCCTCTGAC 
GATGAGAAGC TGAAGCGCTG CACACGCTGT TATCGTGTCG GCTACTGCAA TCAGACCTGC 
CAGAAGAACC ACTGGCCTTC TCACAAGACT ATGTGTCGGT CTAATGTGGA AAACATTGGA 
CAGCCATTTT TAATCAGCGT GCCTGAGTCT AGACTTTCTT ACACTCGCCT CACACAGCTT 
CTAGAAGGCT ACTCTAGGTA CTCTGTCAAT GTGTTCCAGC CACCTTTCCA GTCAGGTCGG 
ACGTCCCCTG AGTCCAGTCT GTCCCGTGTT GACCTGACTT CCATGGCGAG CAGCGTCCCA 
GAGTGTCAAG AGAAGGACCT GCCATCAGCA GGTGACCCAG AAAACCCAGC AGAGCAGGAT 
GAATCTGTCA CAGCCTCACA AACGGAGAGT GCAGCAGATG GAGGAGACAC TCTCTCCACA 
CATACCACTG ACTCTGGTTG CTGTGAGCTC GCCTCCAGCT CCCATGATTC TCTGCTGGAG 
AAAGAAACGT CATGTGAAAA GGCTGTTAAA CCAGAAGCTG TAGTAACTGG GTATCAACAG 
CCTTCTGAGT CGGCGAGTGG AGGTGCATCT CAATTTTACA TCACTATTCT GGACTCGAGC 
CAAAAGGAAG ACAAAAAACT TGAAGAGAAA GGTGACGCGG TACTGGAGCT GCCTGATGAG 
TGCACACTAG AGCTGGTGTG GAAGAACAAC GAGCGGCAGA AGGAATATGT GCTGGTGCGC 
TCTAAAGATC TGGAGTTTGA TGAGGACCCG GGCTCTGCTA CCGAGACCTC CAGGGCAGGA 
CACTTCACCT TAGAGCAGTG CCTCAACCTC TTTACCAAAC CTGAGGTCCT AGCCCCTGAG 
GAGGCATGGT ACTGTCCTAA GTGTCAGCAG CACAGAGAGG CCTCTAAACA GCTCCTACTT 
TGGCGTCTTC CGAATGTACT CATCATCCAG CTCAAGCGCT TCTCATTTAG GAGTTTCATA 
TGGAGGGACA AGATTAATGA CATGGTCGAT TTTCCAGTCA GAAACTTGGA CTTGAGTAAG 
TTTTGTATTG GTCATAAGGG TGACATCCAG CAGCCGCCAA TCTATGATCT GTACGCTGTC 
ATCAACCATT ATGGTGGAAT GATTGGAGGA CACTACACAG CCTATGCTCG CCTTCCCAGC 
GACAAGAACA GCCAGCTTAG TGATGTTGGC TGGCGTTTGT TTGATGACAG CACAGTCACG 
ACGGTGGAGG AAAGTCAGGT GGTGACGCGT TACGCCTATG TGCTGTTCTA TCGACGCAGG 
AACTCTCCAG TGGAAAGGCC GTCTCACCTG CTGGGACCCC TTGGCGCTGA ATCGTCTGCT 
GCCACAGGTG GTGCTGCCAG TCAGGCGTCT CTGATATGGC AGGAACTGGA GGAGGATGAG 
GAGGGGCAGC GAGAGGCCTC TTTTCGGAGC CTGTTCCGCC CCGGTCTGCG CGGAAGAAGA 
GCCAGGATGA GACCTGAGCA AGAAGAAGAA GAGGAGGGCA GGGGAGGGCT TTCTCACCGC 
AGACGAGATG ACAACTCAGA TTATTCTGAT GGCGAACGCA TGAGATATTT CATCTTGGGC 
ACTGTGGCGG CAATCCTAGC ATTGCTGTTC AATCTCATCT ATCCTTTGTT GTACTGGTCC 
AACTGGGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162176.1
CDS	539..4990
Translation	MASSSTGSSESGRRRGQRGPDDTVSTSKKKQKDRANQESKEAKRVATSTETKKDLFLDWKQNAYEVIVRLNCGGAGELRVEDIEYDFSNSACHIRLPDGREWSCHLHEEIEASCSKLNYKEKSNVLQLVMHKKIPLNTWPAYAKKSEPVNILQKNGSNNYQSSTGRSEKSSEAIEKTPPVSSAEQKRGKPDRGLKRGMKGKQVHVTKSTGEKTPEIKSSDKADSINEPSAKRTTRPSKNTKEPPLAPCLGNESKSKATVNGKTHTSEVNSGASSSSNAKDNRAQMQGKPQGCQALQKEKGIDRKPKSNAQVGSGESKSVSVCEPQVPQKDHSTPVKTCTDKIASDEPENRKEETKPQAEGHIPKMLPSHDLGSGEPASTDSKREESPQKSHTEEKRDKSKEEPQGASQEEDTEGPEPMVDLKFVKNDSYEKGTDLMVVNVYMKEICRQTSRVLFREQDFTLIFQTSDPNFLRLHSDCGPNTVFKWQVKLRNLIQPDQSNYAFTPSRIDITLKKRHSQRWGGLEAPATQVGGAKVAVASSPSTLEKSQPGSSQHALPAKEEPRVGEEKPKPPRAPEDSGLDAVSPRSVSEHVSLKQEPAVATPKPTCMVQPMTHTPPVGNERSEEVEEKKVCHPGFTGLVNLGNTCFMNSVIQSLSNTRELRDYFHDRGFESEINCNNPLGTGGRLAIGFAVLLRALWKGTHHAFQPSKLKAIVASKASQFTGYAQHDAQEFMAFLLDGLHEDLNRIQNKPYTETVDSDGRQDEVVAEEAWQRHKMRNDSFIVDLFQGQYKSKLVCPMCSKVSITFDPFLYLPVPLPQKQKVLTVFYFAKEPHKKPVKFLVSVSKESSSTAEVLESISRSVRIKPENLRLTEVVKGRFHRIFSPSQSLDTVSSSDLLFCFEMLSKDLIKERVVLLKVQQRPQIPSIPITKCAGCLKPPASDDEKLKRCTRCYRVGYCNQTCQKNHWPSHKTMCRSNVENIGQPFLISVPESRLSYTRLTQLLEGYSRYSVNVFQPPFQSGRTSPESSLSRVDLTSMASSVPECQEKDLPSAGDPENPAEQDESVTASQTESAADGGDTLSTHTTDSGCCELASSSHDSLLEKETSCEKAVKPEAVVTGYQQPSESASGGASQFYITILDSSQKEDKKLEEKGDAVLELPDECTLELVWKNNERQKEYVLVRSKDLEFDEDPGSATETSRAGHFTLEQCLNLFTKPEVLAPEEAWYCPKCQQHREASKQLLLWRLPNVLIIQLKRFSFRSFIWRDKINDMVDFPVRNLDLSKFCIGHKGDIQQPPIYDLYAVINHYGGMIGGHYTAYARLPSDKNSQLSDVGWRLFDDSTVTTVEESQVVTRYAYVLFYRRRNSPVERPSHLLGPLGAESSAATGGAASQASLIWQELEEDEEGQREASFRSLFRPGLRGRRARMRPEQEEEEEGRGGLSHRRRDDNSDYSDGERMRYFILGTVAAILALLFNLIYPLLYWSNWV

Target ORF information:

RefSeq Version	XM_005162119.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 19 (usp19), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162119.3

ORF Insert Sequence:

ATGGCCAGCA GCAGCACAGG ATCCAGCGAA TCAGGCCGCA GGCGAGGTCA GAGGGGACCG 
GATGACACGG TCAGCACCAG CAAGAAGAAG CAGAAGGACA GAGCCAACCA GGAGAGCAAA 
GAGGCCAAAC GGGTGGCTAC CAGCACAGAG ACTAAGAAAG ACTTGTTTCT GGACTGGAAG 
CAGAATGCAT ACGAGGTGAT AGTGAGGCTG AACTGTGGAG GTGCTGGGGA GCTGAGAGTA 
GAAGATATCG AGTATGACTT TTCAAATTCC GCCTGCCACA TCCGGCTGCC AGATGGACGT 
GAATGGAGCT GTCATCTCCA TGAAGAGATT GAAGCCTCCT GCAGTAAATT GAATTATAAG 
GAGAAATCGA ATGTCCTGCA GCTTGTCATG CACAAGAAGA TCCCGCTCAA CACCTGGCCC 
GCCTACGCTA AAAAGTCAGA ACCAGTGAAC ATTCTTCAAA AAAATGGAAG CAACAACTAC 
CAGTCAAGCA CAGGACGGTC TGAAAAATCT AGCGAAGCCA TTGAGAAAAC GCCCCCAGTG 
AGCTCTGCTG AGCAGAAACG AGGTAAACCT GACCGAGGGC TGAAACGAGG GATGAAAGGC 
AAGCAGGTGC ACGTCACTAA GAGCACAGGA GAAAAAACGC CAGAGATTAA ATCAAGCGAT 
AAAGCAGACT CGATCAATGA GCCCAGTGCA AAGCGCACCA CGCGGCCCTC TAAAAACACA 
AAGGAGCCCC CCCTAGCTCC CTGCTTGGGA AACGAGTCGA AGTCCAAAGC AACAGTTAAT 
GGGAAGACGC ACACATCAGA GGTTAACAGC GGTGCCTCTT CATCAAGCAA TGCAAAAGAC 
AACAGGGCTC AGATGCAGGG CAAACCTCAA GGATGCCAGG CATTGCAAAA AGAAAAAGGG 
ATTGACAGGA AGCCAAAGAG CAATGCTCAG GTTGGATCAG GGGAAAGCAA GAGCGTCTCT 
GTGTGTGAGC CGCAGGTACC GCAAAAAGAC CATTCTACTC CTGTAAAAAC ATGCACTGAT 
AAAATAGCGT CTGATGAGCC AGAAAACAGA AAGGAGGAAA CGAAGCCACA GGCTGAAGGC 
CACATCCCTA AAATGCTTCC CTCTCATGAC TTGGGATCTG GAGAGCCTGC ATCAACAGAC 
TCCAAAAGAG AGGAGTCTCC ACAAAAGAGC CACACAGAGG AGAAGAGGGA CAAGTCCAAA 
GAGGAGCCAC AAGGAGCAAG CCAAGAGGAG GATACGGAAG GTCCGGAGCC AATGGTGGAC 
TTGAAGTTTG TGAAGAATGA CTCGTATGAG AAGGGAACAG ATCTGATGGT TGTGAATGTC 
TACATGAAAG AAATCTGCCG GCAGACGTCC AGGGTGCTGT TCAGGGAACA AGACTTCACT 
CTTATCTTCC AGACCAGTGA TCCCAACTTT TTGCGCCTTC ATTCGGATTG CGGACCAAAC 
ACTGTCTTTA AATGGCAGGT TAAACTCAGG AATCTAATTC AGCCCGATCA GTCCAACTAC 
GCCTTCACTC CGTCCCGTAT CGACATCACC CTGAAGAAGA GACACAGTCA GCGCTGGGGG 
GGTCTGGAGG CCCCTGCCAC ACAAGTGGGG GGCGCCAAGG TTGCTGTGGC CTCCAGCCCT 
TCTACACTTG AAAAGAGTCA GCCAGGAAGC AGCCAGCATG CACTTCCTGC AAAAGAAGAA 
CCACGGGTTG GAGAGGAGAA ACCCAAACCC CCTAGAGCCC CAGAGGACTC TGGTTTGGAT 
GCTGTGTCCC CACGCTCAGT TTCCGAACAT GTGTCCCTCA AACAAGAACC TGCTGTTGCC 
ACGCCCAAAC CCACCTGCAT GGTGCAGCCC ATGACCCACA CTCCTCCGGT AGGCAATGAG 
AGATCAGAAG AGGTGGAGGA GAAAAAAGTA TGTCACCCTG GCTTCACTGG ACTGGTCAAT 
CTGGGAAACA CTTGCTTTAT GAACAGTGTG ATCCAGTCCC TCTCCAACAC ACGGGAGCTC 
AGGGATTATT TCCATGATCG AGGTTTTGAG TCAGAGATTA ATTGTAATAA CCCATTGGGC 
ACGGGTGGAC GGCTGGCCAT TGGCTTTGCT GTTCTGCTAC GGGCGCTGTG GAAGGGCACT 
CATCATGCTT TTCAACCCTC CAAATTAAAG GCTATCGTTG CCAGTAAAGC CAGTCAGTTT 
ACAGGTTATG CGCAGCATGA TGCTCAGGAG TTTATGGCTT TTTTGCTGGA TGGTCTTCAT 
GAAGACCTGA ACCGCATCCA AAACAAACCA TACACCGAGA CTGTGGACTC AGACGGCCGT 
CAGGATGAAG TTGTTGCAGA AGAGGCATGG CAGAGGCATA AAATGAGAAA CGATTCCTTC 
ATTGTTGACC TCTTTCAAGG CCAGTACAAG TCAAAGCTGG TGTGTCCAAT GTGCTCCAAG 
GTTTCCATAA CCTTTGATCC ATTCTTGTAC TTGCCAGTCC CTCTACCTCA GAAGCAGAAG 
GTGCTGACTG TTTTCTATTT TGCTAAAGAG CCTCACAAGA AACCAGTCAA GTTCTTGGTT 
AGTGTGAGTA AAGAGAGCTC AAGCACGGCT GAAGTACTGG AGTCAATATC TCGCAGCGTG 
AGGATCAAAC CAGAGAACCT GAGGCTGACA GAGGTTGTGA AGGGCAGGTT CCACAGAATC 
TTCTCACCGT CTCAATCTTT AGACACGGTC TCGTCCAGTG ACCTGCTCTT CTGCTTTGAG 
ATGCTGTCAA AAGATCTCAT CAAAGAGAGA GTGGTGTTGC TGAAGGTCCA GCAGAGACCT 
CAGATTCCTA GCATACCAAT CACAAAGTGC GCTGGTTGTC TGAAGCCTCC TGCCTCTGAC 
GATGAGAAGC TGAAGCGCTG CACACGCTGT TATCGTGTCG GCTACTGCAA TCAGACCTGC 
CAGAAGAACC ACTGGCCTTC TCACAAGACT ATGTGTCGGT CTAATGTGGA AAACATTGGA 
CAGCCATTTT TAATCAGCGT GCCTGAGTCT AGACTTTCTT ACACTCGCCT CACACAGCTT 
CTAGAAGGCT ACTCTAGGTA CTCTGTCAAT GTGTTCCAGC CACCTTTCCA GTCAGGTCGG 
ACGTCCCCTG AGTCCAGTCT GTCCCGTGTT GACCTGACTT CCATGGCGAG CAGCGTCCCA 
GAGTGTCAAG AGAAGGACCT GCCATCAGCA GGTGACCCAG AAAACCCAGC AGAGCAGGAT 
GAATCTGTCA CAGCCTCACA AACGGAGAGT GCAGCAGATG GAGGAGACAC TCTCTCCACA 
CATACCACTG ACTCTGGTTG CTGTGAGCTC GCCTCCAGCT CCCATGATTC TCTGCTGGAG 
AAAGAAACGT CATGTGAAAA GGCTGTTAAA CCAGAAGCTG TAGTAACTGG GTATCAACAG 
CCTTCTGAGT CGGCGAGTGG AGGTGCATCT CAATTTTACA TCACTATTCT GGACTCGAGC 
CAAAAGGAAG ACAAAAAACT TGAAGAGAAA GGTGACGCGG TACTGGAGCT GCCTGATGAG 
TGCACACTAG AGCTGGTGTG GAAGAACAAC GAGCGGCAGA AGGAATATGT GCTGGTGCGC 
TCTAAAGATC TGGAGTTTGA TGAGGACCCG GGCTCTGCTA CCGAGACCTC CAGGGCAGGA 
CACTTCACCT TAGAGCAGTG CCTCAACCTC TTTACCAAAC CTGAGGTCCT AGCCCCTGAG 
GAGGCATGGT ACTGTCCTAA GTGTCAGCAG CACAGAGAGG CCTCTAAACA GCTCCTACTT 
TGGCGTCTTC CGAATGTACT CATCATCCAG CTCAAGCGCT TCTCATTTAG GAGTTTCATA 
TGGAGGGACA AGATTAATGA CATGGTCGAT TTTCCAGTCA GAAACTTGGA CTTGAGTAAG 
TTTTGTATTG GTCATAAGGG TGACATCCAG CAGCCGCCAA TCTATGATCT GTACGCTGTC 
ATCAACCATT ATGGTGGAAT GATTGGAGGA CACTACACAG CCTATGCTCG CCTTCCCAGC 
GACAAGAACA GCCAGCTTAG TGATGTTGGC TGGCGTTTGT TTGATGACAG CACAGTCACG 
ACGGTGGAGG AAAGTCAGGT GGTGACGCGT TACGCCTATG TGCTGTTCTA TCGACGCAGG 
AACTCTCCAG TGGAAAGGCC GTCTCACCTG CTGGGACCCC TTGGCGCTGA ATCGTCTGCT 
GCCACAGGTG GTGCTGCCAG TCAGGCGTCT CTGATATGGC AGGAACTGGA GGAGGATGAG 
GAGGGGCAGC GAGAGGCCTC TTTTCGGAGC CTGTTCCGCC CCGGTCTGCG CGGAAGAAGA 
GCCAGGATGA GACCTGAGCA AGAAGAAGAA GAGGAGGGCA GGGGAGGGCT TTCTCACCGC 
AGACGAGATG ACAACTCAGA TTATTCTGAT GGCGAACGCA TGAGATATTT CATCTTGGGC 
ACTGTGGCGG CAATCCTAGC ATTGCTGTTC AATCTCATCT ATCCTTTGTT GTACTGGTCC 
AACTGGGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp19 ( XM_005162119.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162176.1 Danio rerio ubiquitin specific peptidase 19 (usp19), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa27652
Clone ID Related Accession (Same CDS sequence)	XM_005162119.3 , XM_005162119.4
Accession Version	XM_005162119.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4452bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 19 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162119.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCA GCAGCACAGG ATCCAGCGAA TCAGGCCGCA GGCGAGGTCA GAGGGGACCG 
GATGACACGG TCAGCACCAG CAAGAAGAAG CAGAAGGACA GAGCCAACCA GGAGAGCAAA 
GAGGCCAAAC GGGTGGCTAC CAGCACAGAG ACTAAGAAAG ACTTGTTTCT GGACTGGAAG 
CAGAATGCAT ACGAGGTGAT AGTGAGGCTG AACTGTGGAG GTGCTGGGGA GCTGAGAGTA 
GAAGATATCG AGTATGACTT TTCAAATTCC GCCTGCCACA TCCGGCTGCC AGATGGACGT 
GAATGGAGCT GTCATCTCCA TGAAGAGATT GAAGCCTCCT GCAGTAAATT GAATTATAAG 
GAGAAATCGA ATGTCCTGCA GCTTGTCATG CACAAGAAGA TCCCGCTCAA CACCTGGCCC 
GCCTACGCTA AAAAGTCAGA ACCAGTGAAC ATTCTTCAAA AAAATGGAAG CAACAACTAC 
CAGTCAAGCA CAGGACGGTC TGAAAAATCT AGCGAAGCCA TTGAGAAAAC GCCCCCAGTG 
AGCTCTGCTG AGCAGAAACG AGGTAAACCT GACCGAGGGC TGAAACGAGG GATGAAAGGC 
AAGCAGGTGC ACGTCACTAA GAGCACAGGA GAAAAAACGC CAGAGATTAA ATCAAGCGAT 
AAAGCAGACT CGATCAATGA GCCCAGTGCA AAGCGCACCA CGCGGCCCTC TAAAAACACA 
AAGGAGCCCC CCCTAGCTCC CTGCTTGGGA AACGAGTCGA AGTCCAAAGC AACAGTTAAT 
GGGAAGACGC ACACATCAGA GGTTAACAGC GGTGCCTCTT CATCAAGCAA TGCAAAAGAC 
AACAGGGCTC AGATGCAGGG CAAACCTCAA GGATGCCAGG CATTGCAAAA AGAAAAAGGG 
ATTGACAGGA AGCCAAAGAG CAATGCTCAG GTTGGATCAG GGGAAAGCAA GAGCGTCTCT 
GTGTGTGAGC CGCAGGTACC GCAAAAAGAC CATTCTACTC CTGTAAAAAC ATGCACTGAT 
AAAATAGCGT CTGATGAGCC AGAAAACAGA AAGGAGGAAA CGAAGCCACA GGCTGAAGGC 
CACATCCCTA AAATGCTTCC CTCTCATGAC TTGGGATCTG GAGAGCCTGC ATCAACAGAC 
TCCAAAAGAG AGGAGTCTCC ACAAAAGAGC CACACAGAGG AGAAGAGGGA CAAGTCCAAA 
GAGGAGCCAC AAGGAGCAAG CCAAGAGGAG GATACGGAAG GTCCGGAGCC AATGGTGGAC 
TTGAAGTTTG TGAAGAATGA CTCGTATGAG AAGGGAACAG ATCTGATGGT TGTGAATGTC 
TACATGAAAG AAATCTGCCG GCAGACGTCC AGGGTGCTGT TCAGGGAACA AGACTTCACT 
CTTATCTTCC AGACCAGTGA TCCCAACTTT TTGCGCCTTC ATTCGGATTG CGGACCAAAC 
ACTGTCTTTA AATGGCAGGT TAAACTCAGG AATCTAATTC AGCCCGATCA GTCCAACTAC 
GCCTTCACTC CGTCCCGTAT CGACATCACC CTGAAGAAGA GACACAGTCA GCGCTGGGGG 
GGTCTGGAGG CCCCTGCCAC ACAAGTGGGG GGCGCCAAGG TTGCTGTGGC CTCCAGCCCT 
TCTACACTTG AAAAGAGTCA GCCAGGAAGC AGCCAGCATG CACTTCCTGC AAAAGAAGAA 
CCACGGGTTG GAGAGGAGAA ACCCAAACCC CCTAGAGCCC CAGAGGACTC TGGTTTGGAT 
GCTGTGTCCC CACGCTCAGT TTCCGAACAT GTGTCCCTCA AACAAGAACC TGCTGTTGCC 
ACGCCCAAAC CCACCTGCAT GGTGCAGCCC ATGACCCACA CTCCTCCGGT AGGCAATGAG 
AGATCAGAAG AGGTGGAGGA GAAAAAAGTA TGTCACCCTG GCTTCACTGG ACTGGTCAAT 
CTGGGAAACA CTTGCTTTAT GAACAGTGTG ATCCAGTCCC TCTCCAACAC ACGGGAGCTC 
AGGGATTATT TCCATGATCG AGGTTTTGAG TCAGAGATTA ATTGTAATAA CCCATTGGGC 
ACGGGTGGAC GGCTGGCCAT TGGCTTTGCT GTTCTGCTAC GGGCGCTGTG GAAGGGCACT 
CATCATGCTT TTCAACCCTC CAAATTAAAG GCTATCGTTG CCAGTAAAGC CAGTCAGTTT 
ACAGGTTATG CGCAGCATGA TGCTCAGGAG TTTATGGCTT TTTTGCTGGA TGGTCTTCAT 
GAAGACCTGA ACCGCATCCA AAACAAACCA TACACCGAGA CTGTGGACTC AGACGGCCGT 
CAGGATGAAG TTGTTGCAGA AGAGGCATGG CAGAGGCATA AAATGAGAAA CGATTCCTTC 
ATTGTTGACC TCTTTCAAGG CCAGTACAAG TCAAAGCTGG TGTGTCCAAT GTGCTCCAAG 
GTTTCCATAA CCTTTGATCC ATTCTTGTAC TTGCCAGTCC CTCTACCTCA GAAGCAGAAG 
GTGCTGACTG TTTTCTATTT TGCTAAAGAG CCTCACAAGA AACCAGTCAA GTTCTTGGTT 
AGTGTGAGTA AAGAGAGCTC AAGCACGGCT GAAGTACTGG AGTCAATATC TCGCAGCGTG 
AGGATCAAAC CAGAGAACCT GAGGCTGACA GAGGTTGTGA AGGGCAGGTT CCACAGAATC 
TTCTCACCGT CTCAATCTTT AGACACGGTC TCGTCCAGTG ACCTGCTCTT CTGCTTTGAG 
ATGCTGTCAA AAGATCTCAT CAAAGAGAGA GTGGTGTTGC TGAAGGTCCA GCAGAGACCT 
CAGATTCCTA GCATACCAAT CACAAAGTGC GCTGGTTGTC TGAAGCCTCC TGCCTCTGAC 
GATGAGAAGC TGAAGCGCTG CACACGCTGT TATCGTGTCG GCTACTGCAA TCAGACCTGC 
CAGAAGAACC ACTGGCCTTC TCACAAGACT ATGTGTCGGT CTAATGTGGA AAACATTGGA 
CAGCCATTTT TAATCAGCGT GCCTGAGTCT AGACTTTCTT ACACTCGCCT CACACAGCTT 
CTAGAAGGCT ACTCTAGGTA CTCTGTCAAT GTGTTCCAGC CACCTTTCCA GTCAGGTCGG 
ACGTCCCCTG AGTCCAGTCT GTCCCGTGTT GACCTGACTT CCATGGCGAG CAGCGTCCCA 
GAGTGTCAAG AGAAGGACCT GCCATCAGCA GGTGACCCAG AAAACCCAGC AGAGCAGGAT 
GAATCTGTCA CAGCCTCACA AACGGAGAGT GCAGCAGATG GAGGAGACAC TCTCTCCACA 
CATACCACTG ACTCTGGTTG CTGTGAGCTC GCCTCCAGCT CCCATGATTC TCTGCTGGAG 
AAAGAAACGT CATGTGAAAA GGCTGTTAAA CCAGAAGCTG TAGTAACTGG GTATCAACAG 
CCTTCTGAGT CGGCGAGTGG AGGTGCATCT CAATTTTACA TCACTATTCT GGACTCGAGC 
CAAAAGGAAG ACAAAAAACT TGAAGAGAAA GGTGACGCGG TACTGGAGCT GCCTGATGAG 
TGCACACTAG AGCTGGTGTG GAAGAACAAC GAGCGGCAGA AGGAATATGT GCTGGTGCGC 
TCTAAAGATC TGGAGTTTGA TGAGGACCCG GGCTCTGCTA CCGAGACCTC CAGGGCAGGA 
CACTTCACCT TAGAGCAGTG CCTCAACCTC TTTACCAAAC CTGAGGTCCT AGCCCCTGAG 
GAGGCATGGT ACTGTCCTAA GTGTCAGCAG CACAGAGAGG CCTCTAAACA GCTCCTACTT 
TGGCGTCTTC CGAATGTACT CATCATCCAG CTCAAGCGCT TCTCATTTAG GAGTTTCATA 
TGGAGGGACA AGATTAATGA CATGGTCGAT TTTCCAGTCA GAAACTTGGA CTTGAGTAAG 
TTTTGTATTG GTCATAAGGG TGACATCCAG CAGCCGCCAA TCTATGATCT GTACGCTGTC 
ATCAACCATT ATGGTGGAAT GATTGGAGGA CACTACACAG CCTATGCTCG CCTTCCCAGC 
GACAAGAACA GCCAGCTTAG TGATGTTGGC TGGCGTTTGT TTGATGACAG CACAGTCACG 
ACGGTGGAGG AAAGTCAGGT GGTGACGCGT TACGCCTATG TGCTGTTCTA TCGACGCAGG 
AACTCTCCAG TGGAAAGGCC GTCTCACCTG CTGGGACCCC TTGGCGCTGA ATCGTCTGCT 
GCCACAGGTG GTGCTGCCAG TCAGGCGTCT CTGATATGGC AGGAACTGGA GGAGGATGAG 
GAGGGGCAGC GAGAGGCCTC TTTTCGGAGC CTGTTCCGCC CCGGTCTGCG CGGAAGAAGA 
GCCAGGATGA GACCTGAGCA AGAAGAAGAA GAGGAGGGCA GGGGAGGGCT TTCTCACCGC 
AGACGAGATG ACAACTCAGA TTATTCTGAT GGCGAACGCA TGAGATATTT CATCTTGGGC 
ACTGTGGCGG CAATCCTAGC ATTGCTGTTC AATCTCATCT ATCCTTTGTT GTACTGGTCC 
AACTGGGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162176.1
CDS	540..4991
Translation	MASSSTGSSESGRRRGQRGPDDTVSTSKKKQKDRANQESKEAKRVATSTETKKDLFLDWKQNAYEVIVRLNCGGAGELRVEDIEYDFSNSACHIRLPDGREWSCHLHEEIEASCSKLNYKEKSNVLQLVMHKKIPLNTWPAYAKKSEPVNILQKNGSNNYQSSTGRSEKSSEAIEKTPPVSSAEQKRGKPDRGLKRGMKGKQVHVTKSTGEKTPEIKSSDKADSINEPSAKRTTRPSKNTKEPPLAPCLGNESKSKATVNGKTHTSEVNSGASSSSNAKDNRAQMQGKPQGCQALQKEKGIDRKPKSNAQVGSGESKSVSVCEPQVPQKDHSTPVKTCTDKIASDEPENRKEETKPQAEGHIPKMLPSHDLGSGEPASTDSKREESPQKSHTEEKRDKSKEEPQGASQEEDTEGPEPMVDLKFVKNDSYEKGTDLMVVNVYMKEICRQTSRVLFREQDFTLIFQTSDPNFLRLHSDCGPNTVFKWQVKLRNLIQPDQSNYAFTPSRIDITLKKRHSQRWGGLEAPATQVGGAKVAVASSPSTLEKSQPGSSQHALPAKEEPRVGEEKPKPPRAPEDSGLDAVSPRSVSEHVSLKQEPAVATPKPTCMVQPMTHTPPVGNERSEEVEEKKVCHPGFTGLVNLGNTCFMNSVIQSLSNTRELRDYFHDRGFESEINCNNPLGTGGRLAIGFAVLLRALWKGTHHAFQPSKLKAIVASKASQFTGYAQHDAQEFMAFLLDGLHEDLNRIQNKPYTETVDSDGRQDEVVAEEAWQRHKMRNDSFIVDLFQGQYKSKLVCPMCSKVSITFDPFLYLPVPLPQKQKVLTVFYFAKEPHKKPVKFLVSVSKESSSTAEVLESISRSVRIKPENLRLTEVVKGRFHRIFSPSQSLDTVSSSDLLFCFEMLSKDLIKERVVLLKVQQRPQIPSIPITKCAGCLKPPASDDEKLKRCTRCYRVGYCNQTCQKNHWPSHKTMCRSNVENIGQPFLISVPESRLSYTRLTQLLEGYSRYSVNVFQPPFQSGRTSPESSLSRVDLTSMASSVPECQEKDLPSAGDPENPAEQDESVTASQTESAADGGDTLSTHTTDSGCCELASSSHDSLLEKETSCEKAVKPEAVVTGYQQPSESASGGASQFYITILDSSQKEDKKLEEKGDAVLELPDECTLELVWKNNERQKEYVLVRSKDLEFDEDPGSATETSRAGHFTLEQCLNLFTKPEVLAPEEAWYCPKCQQHREASKQLLLWRLPNVLIIQLKRFSFRSFIWRDKINDMVDFPVRNLDLSKFCIGHKGDIQQPPIYDLYAVINHYGGMIGGHYTAYARLPSDKNSQLSDVGWRLFDDSTVTTVEESQVVTRYAYVLFYRRRNSPVERPSHLLGPLGAESSAATGGAASQASLIWQELEEDEEGQREASFRSLFRPGLRGRRARMRPEQEEEEEGRGGLSHRRRDDNSDYSDGERMRYFILGTVAAILALLFNLIYPLLYWSNWV

Target ORF information:

RefSeq Version	XM_005162119.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 19 (usp19), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162119.4

ORF Insert Sequence:

ATGGCCAGCA GCAGCACAGG ATCCAGCGAA TCAGGCCGCA GGCGAGGTCA GAGGGGACCG 
GATGACACGG TCAGCACCAG CAAGAAGAAG CAGAAGGACA GAGCCAACCA GGAGAGCAAA 
GAGGCCAAAC GGGTGGCTAC CAGCACAGAG ACTAAGAAAG ACTTGTTTCT GGACTGGAAG 
CAGAATGCAT ACGAGGTGAT AGTGAGGCTG AACTGTGGAG GTGCTGGGGA GCTGAGAGTA 
GAAGATATCG AGTATGACTT TTCAAATTCC GCCTGCCACA TCCGGCTGCC AGATGGACGT 
GAATGGAGCT GTCATCTCCA TGAAGAGATT GAAGCCTCCT GCAGTAAATT GAATTATAAG 
GAGAAATCGA ATGTCCTGCA GCTTGTCATG CACAAGAAGA TCCCGCTCAA CACCTGGCCC 
GCCTACGCTA AAAAGTCAGA ACCAGTGAAC ATTCTTCAAA AAAATGGAAG CAACAACTAC 
CAGTCAAGCA CAGGACGGTC TGAAAAATCT AGCGAAGCCA TTGAGAAAAC GCCCCCAGTG 
AGCTCTGCTG AGCAGAAACG AGGTAAACCT GACCGAGGGC TGAAACGAGG GATGAAAGGC 
AAGCAGGTGC ACGTCACTAA GAGCACAGGA GAAAAAACGC CAGAGATTAA ATCAAGCGAT 
AAAGCAGACT CGATCAATGA GCCCAGTGCA AAGCGCACCA CGCGGCCCTC TAAAAACACA 
AAGGAGCCCC CCCTAGCTCC CTGCTTGGGA AACGAGTCGA AGTCCAAAGC AACAGTTAAT 
GGGAAGACGC ACACATCAGA GGTTAACAGC GGTGCCTCTT CATCAAGCAA TGCAAAAGAC 
AACAGGGCTC AGATGCAGGG CAAACCTCAA GGATGCCAGG CATTGCAAAA AGAAAAAGGG 
ATTGACAGGA AGCCAAAGAG CAATGCTCAG GTTGGATCAG GGGAAAGCAA GAGCGTCTCT 
GTGTGTGAGC CGCAGGTACC GCAAAAAGAC CATTCTACTC CTGTAAAAAC ATGCACTGAT 
AAAATAGCGT CTGATGAGCC AGAAAACAGA AAGGAGGAAA CGAAGCCACA GGCTGAAGGC 
CACATCCCTA AAATGCTTCC CTCTCATGAC TTGGGATCTG GAGAGCCTGC ATCAACAGAC 
TCCAAAAGAG AGGAGTCTCC ACAAAAGAGC CACACAGAGG AGAAGAGGGA CAAGTCCAAA 
GAGGAGCCAC AAGGAGCAAG CCAAGAGGAG GATACGGAAG GTCCGGAGCC AATGGTGGAC 
TTGAAGTTTG TGAAGAATGA CTCGTATGAG AAGGGAACAG ATCTGATGGT TGTGAATGTC 
TACATGAAAG AAATCTGCCG GCAGACGTCC AGGGTGCTGT TCAGGGAACA AGACTTCACT 
CTTATCTTCC AGACCAGTGA TCCCAACTTT TTGCGCCTTC ATTCGGATTG CGGACCAAAC 
ACTGTCTTTA AATGGCAGGT TAAACTCAGG AATCTAATTC AGCCCGATCA GTCCAACTAC 
GCCTTCACTC CGTCCCGTAT CGACATCACC CTGAAGAAGA GACACAGTCA GCGCTGGGGG 
GGTCTGGAGG CCCCTGCCAC ACAAGTGGGG GGCGCCAAGG TTGCTGTGGC CTCCAGCCCT 
TCTACACTTG AAAAGAGTCA GCCAGGAAGC AGCCAGCATG CACTTCCTGC AAAAGAAGAA 
CCACGGGTTG GAGAGGAGAA ACCCAAACCC CCTAGAGCCC CAGAGGACTC TGGTTTGGAT 
GCTGTGTCCC CACGCTCAGT TTCCGAACAT GTGTCCCTCA AACAAGAACC TGCTGTTGCC 
ACGCCCAAAC CCACCTGCAT GGTGCAGCCC ATGACCCACA CTCCTCCGGT AGGCAATGAG 
AGATCAGAAG AGGTGGAGGA GAAAAAAGTA TGTCACCCTG GCTTCACTGG ACTGGTCAAT 
CTGGGAAACA CTTGCTTTAT GAACAGTGTG ATCCAGTCCC TCTCCAACAC ACGGGAGCTC 
AGGGATTATT TCCATGATCG AGGTTTTGAG TCAGAGATTA ATTGTAATAA CCCATTGGGC 
ACGGGTGGAC GGCTGGCCAT TGGCTTTGCT GTTCTGCTAC GGGCGCTGTG GAAGGGCACT 
CATCATGCTT TTCAACCCTC CAAATTAAAG GCTATCGTTG CCAGTAAAGC CAGTCAGTTT 
ACAGGTTATG CGCAGCATGA TGCTCAGGAG TTTATGGCTT TTTTGCTGGA TGGTCTTCAT 
GAAGACCTGA ACCGCATCCA AAACAAACCA TACACCGAGA CTGTGGACTC AGACGGCCGT 
CAGGATGAAG TTGTTGCAGA AGAGGCATGG CAGAGGCATA AAATGAGAAA CGATTCCTTC 
ATTGTTGACC TCTTTCAAGG CCAGTACAAG TCAAAGCTGG TGTGTCCAAT GTGCTCCAAG 
GTTTCCATAA CCTTTGATCC ATTCTTGTAC TTGCCAGTCC CTCTACCTCA GAAGCAGAAG 
GTGCTGACTG TTTTCTATTT TGCTAAAGAG CCTCACAAGA AACCAGTCAA GTTCTTGGTT 
AGTGTGAGTA AAGAGAGCTC AAGCACGGCT GAAGTACTGG AGTCAATATC TCGCAGCGTG 
AGGATCAAAC CAGAGAACCT GAGGCTGACA GAGGTTGTGA AGGGCAGGTT CCACAGAATC 
TTCTCACCGT CTCAATCTTT AGACACGGTC TCGTCCAGTG ACCTGCTCTT CTGCTTTGAG 
ATGCTGTCAA AAGATCTCAT CAAAGAGAGA GTGGTGTTGC TGAAGGTCCA GCAGAGACCT 
CAGATTCCTA GCATACCAAT CACAAAGTGC GCTGGTTGTC TGAAGCCTCC TGCCTCTGAC 
GATGAGAAGC TGAAGCGCTG CACACGCTGT TATCGTGTCG GCTACTGCAA TCAGACCTGC 
CAGAAGAACC ACTGGCCTTC TCACAAGACT ATGTGTCGGT CTAATGTGGA AAACATTGGA 
CAGCCATTTT TAATCAGCGT GCCTGAGTCT AGACTTTCTT ACACTCGCCT CACACAGCTT 
CTAGAAGGCT ACTCTAGGTA CTCTGTCAAT GTGTTCCAGC CACCTTTCCA GTCAGGTCGG 
ACGTCCCCTG AGTCCAGTCT GTCCCGTGTT GACCTGACTT CCATGGCGAG CAGCGTCCCA 
GAGTGTCAAG AGAAGGACCT GCCATCAGCA GGTGACCCAG AAAACCCAGC AGAGCAGGAT 
GAATCTGTCA CAGCCTCACA AACGGAGAGT GCAGCAGATG GAGGAGACAC TCTCTCCACA 
CATACCACTG ACTCTGGTTG CTGTGAGCTC GCCTCCAGCT CCCATGATTC TCTGCTGGAG 
AAAGAAACGT CATGTGAAAA GGCTGTTAAA CCAGAAGCTG TAGTAACTGG GTATCAACAG 
CCTTCTGAGT CGGCGAGTGG AGGTGCATCT CAATTTTACA TCACTATTCT GGACTCGAGC 
CAAAAGGAAG ACAAAAAACT TGAAGAGAAA GGTGACGCGG TACTGGAGCT GCCTGATGAG 
TGCACACTAG AGCTGGTGTG GAAGAACAAC GAGCGGCAGA AGGAATATGT GCTGGTGCGC 
TCTAAAGATC TGGAGTTTGA TGAGGACCCG GGCTCTGCTA CCGAGACCTC CAGGGCAGGA 
CACTTCACCT TAGAGCAGTG CCTCAACCTC TTTACCAAAC CTGAGGTCCT AGCCCCTGAG 
GAGGCATGGT ACTGTCCTAA GTGTCAGCAG CACAGAGAGG CCTCTAAACA GCTCCTACTT 
TGGCGTCTTC CGAATGTACT CATCATCCAG CTCAAGCGCT TCTCATTTAG GAGTTTCATA 
TGGAGGGACA AGATTAATGA CATGGTCGAT TTTCCAGTCA GAAACTTGGA CTTGAGTAAG 
TTTTGTATTG GTCATAAGGG TGACATCCAG CAGCCGCCAA TCTATGATCT GTACGCTGTC 
ATCAACCATT ATGGTGGAAT GATTGGAGGA CACTACACAG CCTATGCTCG CCTTCCCAGC 
GACAAGAACA GCCAGCTTAG TGATGTTGGC TGGCGTTTGT TTGATGACAG CACAGTCACG 
ACGGTGGAGG AAAGTCAGGT GGTGACGCGT TACGCCTATG TGCTGTTCTA TCGACGCAGG 
AACTCTCCAG TGGAAAGGCC GTCTCACCTG CTGGGACCCC TTGGCGCTGA ATCGTCTGCT 
GCCACAGGTG GTGCTGCCAG TCAGGCGTCT CTGATATGGC AGGAACTGGA GGAGGATGAG 
GAGGGGCAGC GAGAGGCCTC TTTTCGGAGC CTGTTCCGCC CCGGTCTGCG CGGAAGAAGA 
GCCAGGATGA GACCTGAGCA AGAAGAAGAA GAGGAGGGCA GGGGAGGGCT TTCTCACCGC 
AGACGAGATG ACAACTCAGA TTATTCTGAT GGCGAACGCA TGAGATATTT CATCTTGGGC 
ACTGTGGCGG CAATCCTAGC ATTGCTGTTC AATCTCATCT ATCCTTTGTT GTACTGGTCC 
AACTGGGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp19 ( XM_005162120.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162177.1 Danio rerio ubiquitin specific peptidase 19 (usp19), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa27653
Clone ID Related Accession (Same CDS sequence)	XM_005162120.4 , XM_005162120.3
Accession Version	XM_005162120.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 19 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162120.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCA GCAGCACAGG ATCCAGCGAA TCAGGCCGCA GGCGAGGTCA GAGGGGACCG 
GATGACACGG TCAGCACCAG CAAGAAGAAG CAGAAGGACA GAGCCAACCA GGAGAGCAAA 
GAGGCCAAAC GGGTGGCTAC CAGCACAGAG ACTAAGAAAG ACTTGTTTCT GGACTGGAAG 
CAGAATGCAT ACGAGGTGAT AGTGAGGCTG AACTGTGGAG GTGCTGGGGA GCTGAGAGTA 
GAAGATATCG AGTATGACTT TTCAAATTCC GCCTGCCACA TCCGGCTGCC AGATGGACGT 
GAATGGAGCT GTCATCTCCA TGAAGAGATT GAAGCCTCCT GCAGTAAATT GAATTATAAG 
GAGAAATCGA ATGTCCTGCA GCTTGTCATG CACAAGAAGA TCCCGCTCAA CACCTGGCCC 
GCCTACGCTA AAAAGTCAGA ACCAGTGAAC ATTCTTCAAA AAAATGGAAG CAACAACTAC 
CAGTCAAGCA CAGGACGGTC TGAAAAATCT AGCGAAGCCA TTGAGAAAAC GCCCCCAGTG 
AGCTCTGCTG AGCAGAAACG AGGTAAACCT GACCGAGGGC TGAAACGAGG GATGAAAGGC 
AAGCAGGTGC ACGTCACTAA GAGCACAGGA GAAAAAACGC CAGAGATTAA ATCAAGCGAT 
AAAGCAGACT CGATCAATGA GCCCAGTGCA AAGCGCACCA CGCGGCCCTC TAAAAACACA 
AAGGAGCCCC CCCTAGCTCC CTGCTTGGGA AACGAGTCGA AGTCCAAAGC AACAGTTAAT 
GGGAAGACGC ACACATCAGA GGTTAACAGC GGTGCCTCTT CATCAAGCAA TGCAAAAGAC 
AACAGGGCTC AGATGCAGGG CAAACCTCAA GGATGCCAGG CATTGCAAAA AGAAAAAGGG 
ATTGACAGGA AGCCAAAGAG CAATGCTCAG GTTGGATCAG GGGAAAGCAA GAGCGTCTCT 
GTGTGTGAGC CGCAGGTACC GCAAAAAGAC CATTCTACTC CTGTAAAAAC ATGCACTGAT 
AAAATAGCGT CTGATGAGCC AGAAAACAGA AAGGAGGAAA CGAAGCCACA GGCTGAAGGC 
CACATCCCTA AAATGCTTCC CTCTCATGAC TTGGGATCTG GAGAGCCTGC ATCAACAGAC 
TCCAAAAGAG AGGAGTCTCC ACAAAAGAGC CACACAGAGG AGAAGAGGGA CAAGTCCAAA 
GAGGAGCCAC AAGGAGCAAG CCAAGAGGAG GATACGGAAG GTCCGGAGCC AATGGTGGAC 
TTGAAGTTTG TGAAGAATGA CTCGTATGAG AAGGGAACAG ATCTGATGGT TGTGAATGTC 
TACATGAAAG AAATCTGCCG GCAGACGTCC AGGGTGCTGT TCAGGGAACA AGACTTCACT 
CTTATCTTCC AGACCAGTGA TCCCAACTTT TTGCGCCTTC ATTCGGATTG CGGACCAAAC 
ACTGTCTTTA AATGGCAGGT TAAACTCAGG AATCTAATTC AGCCCGATCA GTCCAACTAC 
GCCTTCACTC CGTCCCGTAT CGACATCACC CTGAAGAAGA GACACAGTCA GCGCTGGGGG 
GGTCTGGAGG CCCCTGCCAC ACAAGGTGCA GTGGGGGGCG CCAAGGTTGC TGTGGCCTCC 
AGCCCTTCTA CACTTGAAAA GAGTCAGCCA GGAAGCAGCC AGCATGCACT TCCTGCAAAA 
GAAGAACCAC GGGTTGGAGA GGAGAAACCC AAACCCCCTA GAGCCCCAGA GGACTCTGGT 
TTGGATGCTG TGTCCCCACG CTCAGTTTCC GAACATGTGT CCCTCAAACA AGAACCTGCT 
GTTGCCACGC CCAAACCCAC CTGCATGGTG CAGCCCATGA CCCACACTCC TCCGGTAGGC 
AATGAGAGAT CAGAAGAGGT GGAGGAGAAA AAAGTATGTC ACCCTGGCTT CACTGGACTG 
GTCAATCTGG GAAACACTTG CTTTATGAAC AGTGTGATCC AGTCCCTCTC CAACACACGG 
GAGCTCAGGG ATTATTTCCA TGATCGAGGT TTTGAGTCAG AGATTAATTG TAATAACCCA 
TTGGGCACGG GTGGACGGCT GGCCATTGGC TTTGCTGTTC TGCTACGGGC GCTGTGGAAG 
GGCACTCATC ATGCTTTTCA ACCCTCCAAA TTAAAGGCTA TCGTTGCCAG TAAAGCCAGT 
CAGTTTACAG GTTATGCGCA GCATGATGCT CAGGAGTTTA TGGCTTTTTT GCTGGATGGT 
CTTCATGAAG ACCTGAACCG CATCCAAAAC AAACCATACA CCGAGACTGT GGACTCAGAC 
GGCCGTCAGG ATGAAGTTGT TGCAGAAGAG GCATGGCAGA GGCATAAAAT GAGAAACGAT 
TCCTTCATTG TTGACCTCTT TCAAGGCCAG TACAAGTCAA AGCTGGTGTG TCCAATGTGC 
TCCAAGGTTT CCATAACCTT TGATCCATTC TTGTACTTGC CAGTCCCTCT ACCTCAGAAG 
CAGAAGGTGC TGACTGTTTT CTATTTTGCT AAAGAGCCTC ACAAGAAACC AGTCAAGTTC 
TTGGTTAGTG TGAGTAAAGA GAGCTCAAGC ACGGCTGAAG TACTGGAGTC AATATCTCGC 
AGCGTGAGGA TCAAACCAGA GAACCTGAGG CTGACAGAGG TTGTGAAGGG CAGGTTCCAC 
AGAATCTTCT CACCGTCTCA ATCTTTAGAC ACGGTCTCGT CCAGTGACCT GCTCTTCTGC 
TTTGAGATGC TGTCAAAAGA TCTCATCAAA GAGAGAGTGG TGTTGCTGAA GGTCCAGCAG 
AGACCTCAGA TTCCTAGCAT ACCAATCACA AAGTGCGCTG GTTGTCTGAA GCCTCCTGCC 
TCTGACGATG AGAAGCTGAA GCGCTGCACA CGCTGTTATC GTGTCGGCTA CTGCAATCAG 
ACCTGCCAGA AGAACCACTG GCCTTCTCAC AAGACTATGT GTCGGTCTAA TGTGGAAAAC 
ATTGGACAGC CATTTTTAAT CAGCGTGCCT GAGTCTAGAC TTTCTTACAC TCGCCTCACA 
CAGCTTCTAG AAGGCTACTC TAGGTACTCT GTCAATGTGT TCCAGCCACC TTTCCAGTCA 
GGTCGGACGT CCCCTGAGTC CAGTCTGTCC CGTGTTGACC TGACTTCCAT GGCGAGCAGC 
GTCCCAGAGT GTCAAGAGAA GGACCTGCCA TCAGCAGGTG ACCCAGAAAA CCCAGCAGAG 
CAGGATGAAT CTGTCACAGC CTCACAAACG GAGAGTGCAG CAGATGGAGG AGACACTCTC 
TCCACACATA CCACTGACTC TGGTTGCTGT GAGCTCGCCT CCAGCTCCCA TGATTCTCTG 
CTGGAGAAAG AAACGTCATG TGAAAAGGCT GTTAAACCAG AAGCTGTAGT AACTGGGTAT 
CAACAGCCTT CTGAGTCGGC GAGTGGAGGT GCATCTCAAT TTTACATCAC TATTCTGGAC 
TCGAGCCAAA AGGAAGACAA AAAACTTGAA GAGAAAGGTG ACGCGGTACT GGAGCTGCCT 
GATGAGTGCA CACTAGAGCT GGTGTGGAAG AACAACGAGC GGCAGAAGGA ATATGTGCTG 
GTGCGCTCTA AAGATCTGGA GTTTGATGAG GACCCGGGCT CTGCTACCGA GACCTCCAGG 
GCAGGACACT TCACCTTAGA GCAGTGCCTC AACCTCTTTA CCAAACCTGA GGTCCTAGCC 
CCTGAGGAGG CATGGTACTG TCCTAAGTGT CAGCAGCACA GAGAGGCCTC TAAACAGCTC 
CTACTTTGGC GTCTTCCGAA TGTACTCATC ATCCAGCTCA AGCGCTTCTC ATTTAGGAGT 
TTCATATGGA GGGACAAGAT TAATGACATG GTCGATTTTC CAGTCAGAAA CTTGGACTTG 
AGTAAGTTTT GTATTGGTCA TAAGGGTGAC ATCCAGCAGC CGCCAATCTA TGATCTGTAC 
GCTGTCATCA ACCATTATGG TGGAATGATT GGAGGACACT ACACAGCCTA TGCTCGCCTT 
CCCAGCGACA AGAACAGCCA GCTTAGTGAT GTTGGCTGGC GTTTGTTTGA TGACAGCACA 
GTCACGACGG TGGAGGAAAG TCAGGTGGTG ACGCGTTACG CCTATGTGCT GTTCTATCGA 
CGCAGGAACT CTCCAGTGGA AAGGCCGTCT CACCTGCTGG GACCCCTTGG CGCTGAATCG 
TCTGCTGCCA CAGGTGGTGC TGCCAGTCAG GCTTCTAGCC AGACACTGTT TGGGACAGAC 
CTGGATCCTG ACGGGCCCCC TCAGTTGAGC ACGGAAGTGA CCTCAGACAT GTTTGCACAC 
TCTGGAGAAT GTGCCGCCCC GTCCTACAGC AGCATGGAGG AGGTGGATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162177.1
CDS	539..4849
Translation	MASSSTGSSESGRRRGQRGPDDTVSTSKKKQKDRANQESKEAKRVATSTETKKDLFLDWKQNAYEVIVRLNCGGAGELRVEDIEYDFSNSACHIRLPDGREWSCHLHEEIEASCSKLNYKEKSNVLQLVMHKKIPLNTWPAYAKKSEPVNILQKNGSNNYQSSTGRSEKSSEAIEKTPPVSSAEQKRGKPDRGLKRGMKGKQVHVTKSTGEKTPEIKSSDKADSINEPSAKRTTRPSKNTKEPPLAPCLGNESKSKATVNGKTHTSEVNSGASSSSNAKDNRAQMQGKPQGCQALQKEKGIDRKPKSNAQVGSGESKSVSVCEPQVPQKDHSTPVKTCTDKIASDEPENRKEETKPQAEGHIPKMLPSHDLGSGEPASTDSKREESPQKSHTEEKRDKSKEEPQGASQEEDTEGPEPMVDLKFVKNDSYEKGTDLMVVNVYMKEICRQTSRVLFREQDFTLIFQTSDPNFLRLHSDCGPNTVFKWQVKLRNLIQPDQSNYAFTPSRIDITLKKRHSQRWGGLEAPATQGAVGGAKVAVASSPSTLEKSQPGSSQHALPAKEEPRVGEEKPKPPRAPEDSGLDAVSPRSVSEHVSLKQEPAVATPKPTCMVQPMTHTPPVGNERSEEVEEKKVCHPGFTGLVNLGNTCFMNSVIQSLSNTRELRDYFHDRGFESEINCNNPLGTGGRLAIGFAVLLRALWKGTHHAFQPSKLKAIVASKASQFTGYAQHDAQEFMAFLLDGLHEDLNRIQNKPYTETVDSDGRQDEVVAEEAWQRHKMRNDSFIVDLFQGQYKSKLVCPMCSKVSITFDPFLYLPVPLPQKQKVLTVFYFAKEPHKKPVKFLVSVSKESSSTAEVLESISRSVRIKPENLRLTEVVKGRFHRIFSPSQSLDTVSSSDLLFCFEMLSKDLIKERVVLLKVQQRPQIPSIPITKCAGCLKPPASDDEKLKRCTRCYRVGYCNQTCQKNHWPSHKTMCRSNVENIGQPFLISVPESRLSYTRLTQLLEGYSRYSVNVFQPPFQSGRTSPESSLSRVDLTSMASSVPECQEKDLPSAGDPENPAEQDESVTASQTESAADGGDTLSTHTTDSGCCELASSSHDSLLEKETSCEKAVKPEAVVTGYQQPSESASGGASQFYITILDSSQKEDKKLEEKGDAVLELPDECTLELVWKNNERQKEYVLVRSKDLEFDEDPGSATETSRAGHFTLEQCLNLFTKPEVLAPEEAWYCPKCQQHREASKQLLLWRLPNVLIIQLKRFSFRSFIWRDKINDMVDFPVRNLDLSKFCIGHKGDIQQPPIYDLYAVINHYGGMIGGHYTAYARLPSDKNSQLSDVGWRLFDDSTVTTVEESQVVTRYAYVLFYRRRNSPVERPSHLLGPLGAESSAATGGAASQASSQTLFGTDLDPDGPPQLSTEVTSDMFAHSGECAAPSYSSMEEVD

Target ORF information:

RefSeq Version	XM_005162120.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 19 (usp19), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162120.4

ORF Insert Sequence:

ATGGCCAGCA GCAGCACAGG ATCCAGCGAA TCAGGCCGCA GGCGAGGTCA GAGGGGACCG 
GATGACACGG TCAGCACCAG CAAGAAGAAG CAGAAGGACA GAGCCAACCA GGAGAGCAAA 
GAGGCCAAAC GGGTGGCTAC CAGCACAGAG ACTAAGAAAG ACTTGTTTCT GGACTGGAAG 
CAGAATGCAT ACGAGGTGAT AGTGAGGCTG AACTGTGGAG GTGCTGGGGA GCTGAGAGTA 
GAAGATATCG AGTATGACTT TTCAAATTCC GCCTGCCACA TCCGGCTGCC AGATGGACGT 
GAATGGAGCT GTCATCTCCA TGAAGAGATT GAAGCCTCCT GCAGTAAATT GAATTATAAG 
GAGAAATCGA ATGTCCTGCA GCTTGTCATG CACAAGAAGA TCCCGCTCAA CACCTGGCCC 
GCCTACGCTA AAAAGTCAGA ACCAGTGAAC ATTCTTCAAA AAAATGGAAG CAACAACTAC 
CAGTCAAGCA CAGGACGGTC TGAAAAATCT AGCGAAGCCA TTGAGAAAAC GCCCCCAGTG 
AGCTCTGCTG AGCAGAAACG AGGTAAACCT GACCGAGGGC TGAAACGAGG GATGAAAGGC 
AAGCAGGTGC ACGTCACTAA GAGCACAGGA GAAAAAACGC CAGAGATTAA ATCAAGCGAT 
AAAGCAGACT CGATCAATGA GCCCAGTGCA AAGCGCACCA CGCGGCCCTC TAAAAACACA 
AAGGAGCCCC CCCTAGCTCC CTGCTTGGGA AACGAGTCGA AGTCCAAAGC AACAGTTAAT 
GGGAAGACGC ACACATCAGA GGTTAACAGC GGTGCCTCTT CATCAAGCAA TGCAAAAGAC 
AACAGGGCTC AGATGCAGGG CAAACCTCAA GGATGCCAGG CATTGCAAAA AGAAAAAGGG 
ATTGACAGGA AGCCAAAGAG CAATGCTCAG GTTGGATCAG GGGAAAGCAA GAGCGTCTCT 
GTGTGTGAGC CGCAGGTACC GCAAAAAGAC CATTCTACTC CTGTAAAAAC ATGCACTGAT 
AAAATAGCGT CTGATGAGCC AGAAAACAGA AAGGAGGAAA CGAAGCCACA GGCTGAAGGC 
CACATCCCTA AAATGCTTCC CTCTCATGAC TTGGGATCTG GAGAGCCTGC ATCAACAGAC 
TCCAAAAGAG AGGAGTCTCC ACAAAAGAGC CACACAGAGG AGAAGAGGGA CAAGTCCAAA 
GAGGAGCCAC AAGGAGCAAG CCAAGAGGAG GATACGGAAG GTCCGGAGCC AATGGTGGAC 
TTGAAGTTTG TGAAGAATGA CTCGTATGAG AAGGGAACAG ATCTGATGGT TGTGAATGTC 
TACATGAAAG AAATCTGCCG GCAGACGTCC AGGGTGCTGT TCAGGGAACA AGACTTCACT 
CTTATCTTCC AGACCAGTGA TCCCAACTTT TTGCGCCTTC ATTCGGATTG CGGACCAAAC 
ACTGTCTTTA AATGGCAGGT TAAACTCAGG AATCTAATTC AGCCCGATCA GTCCAACTAC 
GCCTTCACTC CGTCCCGTAT CGACATCACC CTGAAGAAGA GACACAGTCA GCGCTGGGGG 
GGTCTGGAGG CCCCTGCCAC ACAAGGTGCA GTGGGGGGCG CCAAGGTTGC TGTGGCCTCC 
AGCCCTTCTA CACTTGAAAA GAGTCAGCCA GGAAGCAGCC AGCATGCACT TCCTGCAAAA 
GAAGAACCAC GGGTTGGAGA GGAGAAACCC AAACCCCCTA GAGCCCCAGA GGACTCTGGT 
TTGGATGCTG TGTCCCCACG CTCAGTTTCC GAACATGTGT CCCTCAAACA AGAACCTGCT 
GTTGCCACGC CCAAACCCAC CTGCATGGTG CAGCCCATGA CCCACACTCC TCCGGTAGGC 
AATGAGAGAT CAGAAGAGGT GGAGGAGAAA AAAGTATGTC ACCCTGGCTT CACTGGACTG 
GTCAATCTGG GAAACACTTG CTTTATGAAC AGTGTGATCC AGTCCCTCTC CAACACACGG 
GAGCTCAGGG ATTATTTCCA TGATCGAGGT TTTGAGTCAG AGATTAATTG TAATAACCCA 
TTGGGCACGG GTGGACGGCT GGCCATTGGC TTTGCTGTTC TGCTACGGGC GCTGTGGAAG 
GGCACTCATC ATGCTTTTCA ACCCTCCAAA TTAAAGGCTA TCGTTGCCAG TAAAGCCAGT 
CAGTTTACAG GTTATGCGCA GCATGATGCT CAGGAGTTTA TGGCTTTTTT GCTGGATGGT 
CTTCATGAAG ACCTGAACCG CATCCAAAAC AAACCATACA CCGAGACTGT GGACTCAGAC 
GGCCGTCAGG ATGAAGTTGT TGCAGAAGAG GCATGGCAGA GGCATAAAAT GAGAAACGAT 
TCCTTCATTG TTGACCTCTT TCAAGGCCAG TACAAGTCAA AGCTGGTGTG TCCAATGTGC 
TCCAAGGTTT CCATAACCTT TGATCCATTC TTGTACTTGC CAGTCCCTCT ACCTCAGAAG 
CAGAAGGTGC TGACTGTTTT CTATTTTGCT AAAGAGCCTC ACAAGAAACC AGTCAAGTTC 
TTGGTTAGTG TGAGTAAAGA GAGCTCAAGC ACGGCTGAAG TACTGGAGTC AATATCTCGC 
AGCGTGAGGA TCAAACCAGA GAACCTGAGG CTGACAGAGG TTGTGAAGGG CAGGTTCCAC 
AGAATCTTCT CACCGTCTCA ATCTTTAGAC ACGGTCTCGT CCAGTGACCT GCTCTTCTGC 
TTTGAGATGC TGTCAAAAGA TCTCATCAAA GAGAGAGTGG TGTTGCTGAA GGTCCAGCAG 
AGACCTCAGA TTCCTAGCAT ACCAATCACA AAGTGCGCTG GTTGTCTGAA GCCTCCTGCC 
TCTGACGATG AGAAGCTGAA GCGCTGCACA CGCTGTTATC GTGTCGGCTA CTGCAATCAG 
ACCTGCCAGA AGAACCACTG GCCTTCTCAC AAGACTATGT GTCGGTCTAA TGTGGAAAAC 
ATTGGACAGC CATTTTTAAT CAGCGTGCCT GAGTCTAGAC TTTCTTACAC TCGCCTCACA 
CAGCTTCTAG AAGGCTACTC TAGGTACTCT GTCAATGTGT TCCAGCCACC TTTCCAGTCA 
GGTCGGACGT CCCCTGAGTC CAGTCTGTCC CGTGTTGACC TGACTTCCAT GGCGAGCAGC 
GTCCCAGAGT GTCAAGAGAA GGACCTGCCA TCAGCAGGTG ACCCAGAAAA CCCAGCAGAG 
CAGGATGAAT CTGTCACAGC CTCACAAACG GAGAGTGCAG CAGATGGAGG AGACACTCTC 
TCCACACATA CCACTGACTC TGGTTGCTGT GAGCTCGCCT CCAGCTCCCA TGATTCTCTG 
CTGGAGAAAG AAACGTCATG TGAAAAGGCT GTTAAACCAG AAGCTGTAGT AACTGGGTAT 
CAACAGCCTT CTGAGTCGGC GAGTGGAGGT GCATCTCAAT TTTACATCAC TATTCTGGAC 
TCGAGCCAAA AGGAAGACAA AAAACTTGAA GAGAAAGGTG ACGCGGTACT GGAGCTGCCT 
GATGAGTGCA CACTAGAGCT GGTGTGGAAG AACAACGAGC GGCAGAAGGA ATATGTGCTG 
GTGCGCTCTA AAGATCTGGA GTTTGATGAG GACCCGGGCT CTGCTACCGA GACCTCCAGG 
GCAGGACACT TCACCTTAGA GCAGTGCCTC AACCTCTTTA CCAAACCTGA GGTCCTAGCC 
CCTGAGGAGG CATGGTACTG TCCTAAGTGT CAGCAGCACA GAGAGGCCTC TAAACAGCTC 
CTACTTTGGC GTCTTCCGAA TGTACTCATC ATCCAGCTCA AGCGCTTCTC ATTTAGGAGT 
TTCATATGGA GGGACAAGAT TAATGACATG GTCGATTTTC CAGTCAGAAA CTTGGACTTG 
AGTAAGTTTT GTATTGGTCA TAAGGGTGAC ATCCAGCAGC CGCCAATCTA TGATCTGTAC 
GCTGTCATCA ACCATTATGG TGGAATGATT GGAGGACACT ACACAGCCTA TGCTCGCCTT 
CCCAGCGACA AGAACAGCCA GCTTAGTGAT GTTGGCTGGC GTTTGTTTGA TGACAGCACA 
GTCACGACGG TGGAGGAAAG TCAGGTGGTG ACGCGTTACG CCTATGTGCT GTTCTATCGA 
CGCAGGAACT CTCCAGTGGA AAGGCCGTCT CACCTGCTGG GACCCCTTGG CGCTGAATCG 
TCTGCTGCCA CAGGTGGTGC TGCCAGTCAG GCTTCTAGCC AGACACTGTT TGGGACAGAC 
CTGGATCCTG ACGGGCCCCC TCAGTTGAGC ACGGAAGTGA CCTCAGACAT GTTTGCACAC 
TCTGGAGAAT GTGCCGCCCC GTCCTACAGC AGCATGGAGG AGGTGGATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp19 ( XM_005162120.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162177.1 Danio rerio ubiquitin specific peptidase 19 (usp19), transcript variant X3, mRNA.

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CloneID	ODa27653
Clone ID Related Accession (Same CDS sequence)	XM_005162120.4 , XM_005162120.3
Accession Version	XM_005162120.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 19 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162120.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCA GCAGCACAGG ATCCAGCGAA TCAGGCCGCA GGCGAGGTCA GAGGGGACCG 
GATGACACGG TCAGCACCAG CAAGAAGAAG CAGAAGGACA GAGCCAACCA GGAGAGCAAA 
GAGGCCAAAC GGGTGGCTAC CAGCACAGAG ACTAAGAAAG ACTTGTTTCT GGACTGGAAG 
CAGAATGCAT ACGAGGTGAT AGTGAGGCTG AACTGTGGAG GTGCTGGGGA GCTGAGAGTA 
GAAGATATCG AGTATGACTT TTCAAATTCC GCCTGCCACA TCCGGCTGCC AGATGGACGT 
GAATGGAGCT GTCATCTCCA TGAAGAGATT GAAGCCTCCT GCAGTAAATT GAATTATAAG 
GAGAAATCGA ATGTCCTGCA GCTTGTCATG CACAAGAAGA TCCCGCTCAA CACCTGGCCC 
GCCTACGCTA AAAAGTCAGA ACCAGTGAAC ATTCTTCAAA AAAATGGAAG CAACAACTAC 
CAGTCAAGCA CAGGACGGTC TGAAAAATCT AGCGAAGCCA TTGAGAAAAC GCCCCCAGTG 
AGCTCTGCTG AGCAGAAACG AGGTAAACCT GACCGAGGGC TGAAACGAGG GATGAAAGGC 
AAGCAGGTGC ACGTCACTAA GAGCACAGGA GAAAAAACGC CAGAGATTAA ATCAAGCGAT 
AAAGCAGACT CGATCAATGA GCCCAGTGCA AAGCGCACCA CGCGGCCCTC TAAAAACACA 
AAGGAGCCCC CCCTAGCTCC CTGCTTGGGA AACGAGTCGA AGTCCAAAGC AACAGTTAAT 
GGGAAGACGC ACACATCAGA GGTTAACAGC GGTGCCTCTT CATCAAGCAA TGCAAAAGAC 
AACAGGGCTC AGATGCAGGG CAAACCTCAA GGATGCCAGG CATTGCAAAA AGAAAAAGGG 
ATTGACAGGA AGCCAAAGAG CAATGCTCAG GTTGGATCAG GGGAAAGCAA GAGCGTCTCT 
GTGTGTGAGC CGCAGGTACC GCAAAAAGAC CATTCTACTC CTGTAAAAAC ATGCACTGAT 
AAAATAGCGT CTGATGAGCC AGAAAACAGA AAGGAGGAAA CGAAGCCACA GGCTGAAGGC 
CACATCCCTA AAATGCTTCC CTCTCATGAC TTGGGATCTG GAGAGCCTGC ATCAACAGAC 
TCCAAAAGAG AGGAGTCTCC ACAAAAGAGC CACACAGAGG AGAAGAGGGA CAAGTCCAAA 
GAGGAGCCAC AAGGAGCAAG CCAAGAGGAG GATACGGAAG GTCCGGAGCC AATGGTGGAC 
TTGAAGTTTG TGAAGAATGA CTCGTATGAG AAGGGAACAG ATCTGATGGT TGTGAATGTC 
TACATGAAAG AAATCTGCCG GCAGACGTCC AGGGTGCTGT TCAGGGAACA AGACTTCACT 
CTTATCTTCC AGACCAGTGA TCCCAACTTT TTGCGCCTTC ATTCGGATTG CGGACCAAAC 
ACTGTCTTTA AATGGCAGGT TAAACTCAGG AATCTAATTC AGCCCGATCA GTCCAACTAC 
GCCTTCACTC CGTCCCGTAT CGACATCACC CTGAAGAAGA GACACAGTCA GCGCTGGGGG 
GGTCTGGAGG CCCCTGCCAC ACAAGGTGCA GTGGGGGGCG CCAAGGTTGC TGTGGCCTCC 
AGCCCTTCTA CACTTGAAAA GAGTCAGCCA GGAAGCAGCC AGCATGCACT TCCTGCAAAA 
GAAGAACCAC GGGTTGGAGA GGAGAAACCC AAACCCCCTA GAGCCCCAGA GGACTCTGGT 
TTGGATGCTG TGTCCCCACG CTCAGTTTCC GAACATGTGT CCCTCAAACA AGAACCTGCT 
GTTGCCACGC CCAAACCCAC CTGCATGGTG CAGCCCATGA CCCACACTCC TCCGGTAGGC 
AATGAGAGAT CAGAAGAGGT GGAGGAGAAA AAAGTATGTC ACCCTGGCTT CACTGGACTG 
GTCAATCTGG GAAACACTTG CTTTATGAAC AGTGTGATCC AGTCCCTCTC CAACACACGG 
GAGCTCAGGG ATTATTTCCA TGATCGAGGT TTTGAGTCAG AGATTAATTG TAATAACCCA 
TTGGGCACGG GTGGACGGCT GGCCATTGGC TTTGCTGTTC TGCTACGGGC GCTGTGGAAG 
GGCACTCATC ATGCTTTTCA ACCCTCCAAA TTAAAGGCTA TCGTTGCCAG TAAAGCCAGT 
CAGTTTACAG GTTATGCGCA GCATGATGCT CAGGAGTTTA TGGCTTTTTT GCTGGATGGT 
CTTCATGAAG ACCTGAACCG CATCCAAAAC AAACCATACA CCGAGACTGT GGACTCAGAC 
GGCCGTCAGG ATGAAGTTGT TGCAGAAGAG GCATGGCAGA GGCATAAAAT GAGAAACGAT 
TCCTTCATTG TTGACCTCTT TCAAGGCCAG TACAAGTCAA AGCTGGTGTG TCCAATGTGC 
TCCAAGGTTT CCATAACCTT TGATCCATTC TTGTACTTGC CAGTCCCTCT ACCTCAGAAG 
CAGAAGGTGC TGACTGTTTT CTATTTTGCT AAAGAGCCTC ACAAGAAACC AGTCAAGTTC 
TTGGTTAGTG TGAGTAAAGA GAGCTCAAGC ACGGCTGAAG TACTGGAGTC AATATCTCGC 
AGCGTGAGGA TCAAACCAGA GAACCTGAGG CTGACAGAGG TTGTGAAGGG CAGGTTCCAC 
AGAATCTTCT CACCGTCTCA ATCTTTAGAC ACGGTCTCGT CCAGTGACCT GCTCTTCTGC 
TTTGAGATGC TGTCAAAAGA TCTCATCAAA GAGAGAGTGG TGTTGCTGAA GGTCCAGCAG 
AGACCTCAGA TTCCTAGCAT ACCAATCACA AAGTGCGCTG GTTGTCTGAA GCCTCCTGCC 
TCTGACGATG AGAAGCTGAA GCGCTGCACA CGCTGTTATC GTGTCGGCTA CTGCAATCAG 
ACCTGCCAGA AGAACCACTG GCCTTCTCAC AAGACTATGT GTCGGTCTAA TGTGGAAAAC 
ATTGGACAGC CATTTTTAAT CAGCGTGCCT GAGTCTAGAC TTTCTTACAC TCGCCTCACA 
CAGCTTCTAG AAGGCTACTC TAGGTACTCT GTCAATGTGT TCCAGCCACC TTTCCAGTCA 
GGTCGGACGT CCCCTGAGTC CAGTCTGTCC CGTGTTGACC TGACTTCCAT GGCGAGCAGC 
GTCCCAGAGT GTCAAGAGAA GGACCTGCCA TCAGCAGGTG ACCCAGAAAA CCCAGCAGAG 
CAGGATGAAT CTGTCACAGC CTCACAAACG GAGAGTGCAG CAGATGGAGG AGACACTCTC 
TCCACACATA CCACTGACTC TGGTTGCTGT GAGCTCGCCT CCAGCTCCCA TGATTCTCTG 
CTGGAGAAAG AAACGTCATG TGAAAAGGCT GTTAAACCAG AAGCTGTAGT AACTGGGTAT 
CAACAGCCTT CTGAGTCGGC GAGTGGAGGT GCATCTCAAT TTTACATCAC TATTCTGGAC 
TCGAGCCAAA AGGAAGACAA AAAACTTGAA GAGAAAGGTG ACGCGGTACT GGAGCTGCCT 
GATGAGTGCA CACTAGAGCT GGTGTGGAAG AACAACGAGC GGCAGAAGGA ATATGTGCTG 
GTGCGCTCTA AAGATCTGGA GTTTGATGAG GACCCGGGCT CTGCTACCGA GACCTCCAGG 
GCAGGACACT TCACCTTAGA GCAGTGCCTC AACCTCTTTA CCAAACCTGA GGTCCTAGCC 
CCTGAGGAGG CATGGTACTG TCCTAAGTGT CAGCAGCACA GAGAGGCCTC TAAACAGCTC 
CTACTTTGGC GTCTTCCGAA TGTACTCATC ATCCAGCTCA AGCGCTTCTC ATTTAGGAGT 
TTCATATGGA GGGACAAGAT TAATGACATG GTCGATTTTC CAGTCAGAAA CTTGGACTTG 
AGTAAGTTTT GTATTGGTCA TAAGGGTGAC ATCCAGCAGC CGCCAATCTA TGATCTGTAC 
GCTGTCATCA ACCATTATGG TGGAATGATT GGAGGACACT ACACAGCCTA TGCTCGCCTT 
CCCAGCGACA AGAACAGCCA GCTTAGTGAT GTTGGCTGGC GTTTGTTTGA TGACAGCACA 
GTCACGACGG TGGAGGAAAG TCAGGTGGTG ACGCGTTACG CCTATGTGCT GTTCTATCGA 
CGCAGGAACT CTCCAGTGGA AAGGCCGTCT CACCTGCTGG GACCCCTTGG CGCTGAATCG 
TCTGCTGCCA CAGGTGGTGC TGCCAGTCAG GCTTCTAGCC AGACACTGTT TGGGACAGAC 
CTGGATCCTG ACGGGCCCCC TCAGTTGAGC ACGGAAGTGA CCTCAGACAT GTTTGCACAC 
TCTGGAGAAT GTGCCGCCCC GTCCTACAGC AGCATGGAGG AGGTGGATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162177.1
CDS	538..4848
Translation	MASSSTGSSESGRRRGQRGPDDTVSTSKKKQKDRANQESKEAKRVATSTETKKDLFLDWKQNAYEVIVRLNCGGAGELRVEDIEYDFSNSACHIRLPDGREWSCHLHEEIEASCSKLNYKEKSNVLQLVMHKKIPLNTWPAYAKKSEPVNILQKNGSNNYQSSTGRSEKSSEAIEKTPPVSSAEQKRGKPDRGLKRGMKGKQVHVTKSTGEKTPEIKSSDKADSINEPSAKRTTRPSKNTKEPPLAPCLGNESKSKATVNGKTHTSEVNSGASSSSNAKDNRAQMQGKPQGCQALQKEKGIDRKPKSNAQVGSGESKSVSVCEPQVPQKDHSTPVKTCTDKIASDEPENRKEETKPQAEGHIPKMLPSHDLGSGEPASTDSKREESPQKSHTEEKRDKSKEEPQGASQEEDTEGPEPMVDLKFVKNDSYEKGTDLMVVNVYMKEICRQTSRVLFREQDFTLIFQTSDPNFLRLHSDCGPNTVFKWQVKLRNLIQPDQSNYAFTPSRIDITLKKRHSQRWGGLEAPATQGAVGGAKVAVASSPSTLEKSQPGSSQHALPAKEEPRVGEEKPKPPRAPEDSGLDAVSPRSVSEHVSLKQEPAVATPKPTCMVQPMTHTPPVGNERSEEVEEKKVCHPGFTGLVNLGNTCFMNSVIQSLSNTRELRDYFHDRGFESEINCNNPLGTGGRLAIGFAVLLRALWKGTHHAFQPSKLKAIVASKASQFTGYAQHDAQEFMAFLLDGLHEDLNRIQNKPYTETVDSDGRQDEVVAEEAWQRHKMRNDSFIVDLFQGQYKSKLVCPMCSKVSITFDPFLYLPVPLPQKQKVLTVFYFAKEPHKKPVKFLVSVSKESSSTAEVLESISRSVRIKPENLRLTEVVKGRFHRIFSPSQSLDTVSSSDLLFCFEMLSKDLIKERVVLLKVQQRPQIPSIPITKCAGCLKPPASDDEKLKRCTRCYRVGYCNQTCQKNHWPSHKTMCRSNVENIGQPFLISVPESRLSYTRLTQLLEGYSRYSVNVFQPPFQSGRTSPESSLSRVDLTSMASSVPECQEKDLPSAGDPENPAEQDESVTASQTESAADGGDTLSTHTTDSGCCELASSSHDSLLEKETSCEKAVKPEAVVTGYQQPSESASGGASQFYITILDSSQKEDKKLEEKGDAVLELPDECTLELVWKNNERQKEYVLVRSKDLEFDEDPGSATETSRAGHFTLEQCLNLFTKPEVLAPEEAWYCPKCQQHREASKQLLLWRLPNVLIIQLKRFSFRSFIWRDKINDMVDFPVRNLDLSKFCIGHKGDIQQPPIYDLYAVINHYGGMIGGHYTAYARLPSDKNSQLSDVGWRLFDDSTVTTVEESQVVTRYAYVLFYRRRNSPVERPSHLLGPLGAESSAATGGAASQASSQTLFGTDLDPDGPPQLSTEVTSDMFAHSGECAAPSYSSMEEVD

Target ORF information:

RefSeq Version	XM_005162120.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 19 (usp19), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162120.3

ORF Insert Sequence:

ATGGCCAGCA GCAGCACAGG ATCCAGCGAA TCAGGCCGCA GGCGAGGTCA GAGGGGACCG 
GATGACACGG TCAGCACCAG CAAGAAGAAG CAGAAGGACA GAGCCAACCA GGAGAGCAAA 
GAGGCCAAAC GGGTGGCTAC CAGCACAGAG ACTAAGAAAG ACTTGTTTCT GGACTGGAAG 
CAGAATGCAT ACGAGGTGAT AGTGAGGCTG AACTGTGGAG GTGCTGGGGA GCTGAGAGTA 
GAAGATATCG AGTATGACTT TTCAAATTCC GCCTGCCACA TCCGGCTGCC AGATGGACGT 
GAATGGAGCT GTCATCTCCA TGAAGAGATT GAAGCCTCCT GCAGTAAATT GAATTATAAG 
GAGAAATCGA ATGTCCTGCA GCTTGTCATG CACAAGAAGA TCCCGCTCAA CACCTGGCCC 
GCCTACGCTA AAAAGTCAGA ACCAGTGAAC ATTCTTCAAA AAAATGGAAG CAACAACTAC 
CAGTCAAGCA CAGGACGGTC TGAAAAATCT AGCGAAGCCA TTGAGAAAAC GCCCCCAGTG 
AGCTCTGCTG AGCAGAAACG AGGTAAACCT GACCGAGGGC TGAAACGAGG GATGAAAGGC 
AAGCAGGTGC ACGTCACTAA GAGCACAGGA GAAAAAACGC CAGAGATTAA ATCAAGCGAT 
AAAGCAGACT CGATCAATGA GCCCAGTGCA AAGCGCACCA CGCGGCCCTC TAAAAACACA 
AAGGAGCCCC CCCTAGCTCC CTGCTTGGGA AACGAGTCGA AGTCCAAAGC AACAGTTAAT 
GGGAAGACGC ACACATCAGA GGTTAACAGC GGTGCCTCTT CATCAAGCAA TGCAAAAGAC 
AACAGGGCTC AGATGCAGGG CAAACCTCAA GGATGCCAGG CATTGCAAAA AGAAAAAGGG 
ATTGACAGGA AGCCAAAGAG CAATGCTCAG GTTGGATCAG GGGAAAGCAA GAGCGTCTCT 
GTGTGTGAGC CGCAGGTACC GCAAAAAGAC CATTCTACTC CTGTAAAAAC ATGCACTGAT 
AAAATAGCGT CTGATGAGCC AGAAAACAGA AAGGAGGAAA CGAAGCCACA GGCTGAAGGC 
CACATCCCTA AAATGCTTCC CTCTCATGAC TTGGGATCTG GAGAGCCTGC ATCAACAGAC 
TCCAAAAGAG AGGAGTCTCC ACAAAAGAGC CACACAGAGG AGAAGAGGGA CAAGTCCAAA 
GAGGAGCCAC AAGGAGCAAG CCAAGAGGAG GATACGGAAG GTCCGGAGCC AATGGTGGAC 
TTGAAGTTTG TGAAGAATGA CTCGTATGAG AAGGGAACAG ATCTGATGGT TGTGAATGTC 
TACATGAAAG AAATCTGCCG GCAGACGTCC AGGGTGCTGT TCAGGGAACA AGACTTCACT 
CTTATCTTCC AGACCAGTGA TCCCAACTTT TTGCGCCTTC ATTCGGATTG CGGACCAAAC 
ACTGTCTTTA AATGGCAGGT TAAACTCAGG AATCTAATTC AGCCCGATCA GTCCAACTAC 
GCCTTCACTC CGTCCCGTAT CGACATCACC CTGAAGAAGA GACACAGTCA GCGCTGGGGG 
GGTCTGGAGG CCCCTGCCAC ACAAGGTGCA GTGGGGGGCG CCAAGGTTGC TGTGGCCTCC 
AGCCCTTCTA CACTTGAAAA GAGTCAGCCA GGAAGCAGCC AGCATGCACT TCCTGCAAAA 
GAAGAACCAC GGGTTGGAGA GGAGAAACCC AAACCCCCTA GAGCCCCAGA GGACTCTGGT 
TTGGATGCTG TGTCCCCACG CTCAGTTTCC GAACATGTGT CCCTCAAACA AGAACCTGCT 
GTTGCCACGC CCAAACCCAC CTGCATGGTG CAGCCCATGA CCCACACTCC TCCGGTAGGC 
AATGAGAGAT CAGAAGAGGT GGAGGAGAAA AAAGTATGTC ACCCTGGCTT CACTGGACTG 
GTCAATCTGG GAAACACTTG CTTTATGAAC AGTGTGATCC AGTCCCTCTC CAACACACGG 
GAGCTCAGGG ATTATTTCCA TGATCGAGGT TTTGAGTCAG AGATTAATTG TAATAACCCA 
TTGGGCACGG GTGGACGGCT GGCCATTGGC TTTGCTGTTC TGCTACGGGC GCTGTGGAAG 
GGCACTCATC ATGCTTTTCA ACCCTCCAAA TTAAAGGCTA TCGTTGCCAG TAAAGCCAGT 
CAGTTTACAG GTTATGCGCA GCATGATGCT CAGGAGTTTA TGGCTTTTTT GCTGGATGGT 
CTTCATGAAG ACCTGAACCG CATCCAAAAC AAACCATACA CCGAGACTGT GGACTCAGAC 
GGCCGTCAGG ATGAAGTTGT TGCAGAAGAG GCATGGCAGA GGCATAAAAT GAGAAACGAT 
TCCTTCATTG TTGACCTCTT TCAAGGCCAG TACAAGTCAA AGCTGGTGTG TCCAATGTGC 
TCCAAGGTTT CCATAACCTT TGATCCATTC TTGTACTTGC CAGTCCCTCT ACCTCAGAAG 
CAGAAGGTGC TGACTGTTTT CTATTTTGCT AAAGAGCCTC ACAAGAAACC AGTCAAGTTC 
TTGGTTAGTG TGAGTAAAGA GAGCTCAAGC ACGGCTGAAG TACTGGAGTC AATATCTCGC 
AGCGTGAGGA TCAAACCAGA GAACCTGAGG CTGACAGAGG TTGTGAAGGG CAGGTTCCAC 
AGAATCTTCT CACCGTCTCA ATCTTTAGAC ACGGTCTCGT CCAGTGACCT GCTCTTCTGC 
TTTGAGATGC TGTCAAAAGA TCTCATCAAA GAGAGAGTGG TGTTGCTGAA GGTCCAGCAG 
AGACCTCAGA TTCCTAGCAT ACCAATCACA AAGTGCGCTG GTTGTCTGAA GCCTCCTGCC 
TCTGACGATG AGAAGCTGAA GCGCTGCACA CGCTGTTATC GTGTCGGCTA CTGCAATCAG 
ACCTGCCAGA AGAACCACTG GCCTTCTCAC AAGACTATGT GTCGGTCTAA TGTGGAAAAC 
ATTGGACAGC CATTTTTAAT CAGCGTGCCT GAGTCTAGAC TTTCTTACAC TCGCCTCACA 
CAGCTTCTAG AAGGCTACTC TAGGTACTCT GTCAATGTGT TCCAGCCACC TTTCCAGTCA 
GGTCGGACGT CCCCTGAGTC CAGTCTGTCC CGTGTTGACC TGACTTCCAT GGCGAGCAGC 
GTCCCAGAGT GTCAAGAGAA GGACCTGCCA TCAGCAGGTG ACCCAGAAAA CCCAGCAGAG 
CAGGATGAAT CTGTCACAGC CTCACAAACG GAGAGTGCAG CAGATGGAGG AGACACTCTC 
TCCACACATA CCACTGACTC TGGTTGCTGT GAGCTCGCCT CCAGCTCCCA TGATTCTCTG 
CTGGAGAAAG AAACGTCATG TGAAAAGGCT GTTAAACCAG AAGCTGTAGT AACTGGGTAT 
CAACAGCCTT CTGAGTCGGC GAGTGGAGGT GCATCTCAAT TTTACATCAC TATTCTGGAC 
TCGAGCCAAA AGGAAGACAA AAAACTTGAA GAGAAAGGTG ACGCGGTACT GGAGCTGCCT 
GATGAGTGCA CACTAGAGCT GGTGTGGAAG AACAACGAGC GGCAGAAGGA ATATGTGCTG 
GTGCGCTCTA AAGATCTGGA GTTTGATGAG GACCCGGGCT CTGCTACCGA GACCTCCAGG 
GCAGGACACT TCACCTTAGA GCAGTGCCTC AACCTCTTTA CCAAACCTGA GGTCCTAGCC 
CCTGAGGAGG CATGGTACTG TCCTAAGTGT CAGCAGCACA GAGAGGCCTC TAAACAGCTC 
CTACTTTGGC GTCTTCCGAA TGTACTCATC ATCCAGCTCA AGCGCTTCTC ATTTAGGAGT 
TTCATATGGA GGGACAAGAT TAATGACATG GTCGATTTTC CAGTCAGAAA CTTGGACTTG 
AGTAAGTTTT GTATTGGTCA TAAGGGTGAC ATCCAGCAGC CGCCAATCTA TGATCTGTAC 
GCTGTCATCA ACCATTATGG TGGAATGATT GGAGGACACT ACACAGCCTA TGCTCGCCTT 
CCCAGCGACA AGAACAGCCA GCTTAGTGAT GTTGGCTGGC GTTTGTTTGA TGACAGCACA 
GTCACGACGG TGGAGGAAAG TCAGGTGGTG ACGCGTTACG CCTATGTGCT GTTCTATCGA 
CGCAGGAACT CTCCAGTGGA AAGGCCGTCT CACCTGCTGG GACCCCTTGG CGCTGAATCG 
TCTGCTGCCA CAGGTGGTGC TGCCAGTCAG GCTTCTAGCC AGACACTGTT TGGGACAGAC 
CTGGATCCTG ACGGGCCCCC TCAGTTGAGC ACGGAAGTGA CCTCAGACAT GTTTGCACAC 
TCTGGAGAAT GTGCCGCCCC GTCCTACAGC AGCATGGAGG AGGTGGATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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usp19 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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