ercc3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ercc3
Entrez Gene ID	324323
Full Name	excision repair cross-complementation group 3
Synonyms	wu:fc25f08
General protein information	Preferred Names excision repair cross-complementation group 3 Names TFIIH basal transcription factor complex helicase XPB subunit DNA excision repair protein ERCC-3 excision repair cross-complementing rodent repair deficiency, complementation group 3 xpb
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_201582.1	NP_963876.1	general transcription and DNA repair factor IIH helicase subunit XPB

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_AFR280W	NP_985827.1
Caenorhabditis elegans (roundworm)	Y66D12A.15	NP_499487.1
Arabidopsis thaliana (thale cress)	XPB1	NP_568592.1
Xenopus tropicalis (tropical clawed frog)	ercc3	NP_001123812.1
Canis lupus familiaris (dog)	ERCC3	XP_533314.2
Rattus norvegicus (Norway rat)	Ercc3	NP_001026814.1
Gallus gallus (chicken)	ERCC3	NP_001006523.1
Saccharomyces cerevisiae (baker's yeast)	SSL2	NP_012123.1
Arabidopsis thaliana (thale cress)	XPB2	NP_568591.1
Oryza sativa (rice)	Os01g0691600	NP_001043936.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012169	XP_320364.4
Pan troglodytes (chimpanzee)	ERCC3	XP_525907.3
Drosophila melanogaster (fruit fly)	hay	NP_524020.2
Kluyveromyces lactis	KLLA0E13025g	XP_454537.1
Homo sapiens (human)	ERCC3	NP_000113.1
Bos taurus (cattle)	ERCC3	NP_001039453.1
Mus musculus (house mouse)	Ercc3	NP_598419.1
Danio rerio (zebrafish)	ercc3	NP_963876.1
Magnaporthe oryzae (rice blast fungus)	MGG_06470	XP_003717094.1
Macaca mulatta (Rhesus monkey)	ERCC3	XP_001086722.1
Schizosaccharomyces pombe (fission yeast)	ptr8	NP_593474.1
Neurospora crassa	NCU06438	XP_957329.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Evolutionary conservation of TFIIH subunits: implications for the use of zebrafish as a model to study TFIIH function and regulation.
Silva IA, Cox CJ, Leite RB, Cancela ML, Conceição N
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology172-1739-20(2014 Jun-Jul)

Selection of reliable biomarkers from PCR array analyses using relative distance computational model: methodology and proof-of-concept study.
Liu C, Xu H, Lam SH, Gong Z
PloS one8(12)e83954(2013)

Zebrafish reward mutants reveal novel transcripts mediating the behavioral effects of amphetamine.
Webb KJ, Norton WH, Trümbach D, Meijer AH, Ninkovic J, Topp S, Heck D, Marr C, Wurst W, Theis FJ, Spaink HP, Bally-Cuif L
Genome biology10(7)R81(2009)

The zebrafish gene map defines ancestral vertebrate chromosomes.
Woods IG, Wilson C, Friedlander B, Chang P, Reyes DK, Nix R, Kelly PD, Chu F, Postlethwait JH, Talbot WS
Genome research15(9)1307-14(2005 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ercc3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ercc3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03350	NM_201582.1 Latest version!	Danio rerio excision repair cross-complementation group 3 (ercc3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ercc3 ( NM_201582.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_963876.1 Danio rerio excision repair cross-complementation group 3 (ercc3), mRNA.

Price & Availability↑

CloneID	ODa03350
Clone ID Related Accession (Same CDS sequence)	NM_201582.1
Accession Version	NM_201582.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1542902400000
Organism	Danio rerio(zebrafish)
Product	general transcription and DNA repair factor IIH helicase subunit XPB
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC045400.1. ##Evidence-Data-START## Transcript exon combination :: BC045400.1, BC165665.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGGGAGGA AAGATAAAAG TGACAGAGAG AAGAAGTCCA AGAAACGCTA TTATGAAGAT 
GAAGAGGAGG ATGAAGAGGT GATTGGCGGA GAGTCTCAGG AGGCCGTTCC TGCAGCTGCT 
GGGAAACAGG TGGATGAATC CAGCACTAAA CTGGACGAGT ATGGAGCCAA AGACTACAGG 
CTTCAGATGC TCCTCAAGAA CGACCACTCT TCCCGACCTC TATGGGTGGC TCCTGATGGT 
CACATTTTCC TCGAAGCCTT CTCTCCAGTT TATAAATACG CTCAGGACTT TTTGGTGGCC 
ATTTCAGAGC CGGTGTGTCG ACCCACACAT GCGCATGAGT ACAAACTGAC AGCGTATTCT 
CTGTACGCCG CTGTGAGCGT CGGCCTGCAG ACCTCTGACA TCATTGAGTA CCTGCAGAAA 
CTCAGCAAGA CCTCTGTGCC GGATGGCATA GTGCAGTTTA TTAAGTTGTG TACAGTGAGC 
TATGGTAAAG TCAAACTTGT CCTGAAACAC AACAGATATT TTGTGGAGAG TGCGTTCCCT 
GATGTCATCC AGCGGCTGTT ACAGGACACT GTGATTCGGG ACTGTCGTCT TCGCTCTGCT 
GAGGGTGAGG AGACAGAGCT CATCACCGAG ACCATCTCTA GCAAATCAGC AATCTCAAAG 
TCTCAGCAGG ACAACGGTGG TCCATCGTCC TCTCAGCCTG CAGATGGGCA GCGCTCAGGA 
ACTCAGGTGC CGGAGGACAT CTTCAGTTAC TATGAACAGA TGGATAAAGA GGAGGAGGAG 
GAGGAAGAGA CGCAAACCGT TTCATTTGAG ATCCGTCAGG AAATGATAGA GGAGCTTCAG 
AAGCGCTGTA TTCAGTTGGA GTATCCCCTA CTGGCTGAAT ACGACTTCCG TAATGACACA 
GTGAATCCTG ACATCAACAT GGACCTGAAG CCCACCGCTG TGCTGAGGCC GTATCAGGAA 
AAGAGTCTGA GGAAGATGTT TGGGAATGGC AGAGCTCGAT CTGGAGTCAT CGTCCTGCCT 
TGTGGTGCTG GTAAATCTCT GGTAGGGGTC ACTGCCGCCT GCACGGTGCG CAAACGCTGC 
CTAGTTTTGG GAAACTCCTC TGTGTCAGTG GAACAGTGGA AAGCTCAGTT CAAGATGTGG 
TCCACTATCG ATGACTCCCA GATCTGCCGC TTCACTTCAG ATGCAAAGGA CAAACCCATT 
GGCTGCTCGG TGGCCATCAG CACCTACTCT ATGCTGGGCC ATACCACCAA ACGCTCCTGG 
GAGGCCGAGA GGGTCATGGA GTGGATGAAG AGTCAGGAGT GGGGACTCAT CATACTGGAC 
GAGGTGCACA CCATACCTGC AAAGATGTTC CGTCGTGTTC TTACAATAGT CCAGGCTCAC 
TGTAAACTGG GTCTCACCGC CACTCTGGTA AGAGAAGATG ATAAGATTGT GGATCTGAAC 
TTCCTGATTG GACCAAAGCT CTATGAGGCA AACTGGATGG AACTGCAGAA CAATGGCTAC 
ATCGCTAAAG TCCAGTGTGC TGAGGTGTGG TGTCCCATGT CTCCTGAGTT TTACCGTGAA 
TATGTGGCCA TAAAGACCAA GAAGAGAATT CTGCTCTACA CCATGAATCC CAACAAGTTT 
CGAGCTTGTC AGTTCCTGAT TCGCTTCCAT GAGCGAAGAA ACGACAAGAT CATCGTCTTT 
GCCGACAACG TCTTTGCACT GAAAGAATAC GCCATCCGTC TAAACAAGCC ATATATTTAT 
GGACCCACAT CTCAAGGAGA GCGTATGCAG ATCCTTCAAA ACTTCAAGCA CAATCCCAAA 
ATCAATACGA TCTTCATCTC CAAGGTAGGA GACACTTCAT TTGACCTGCC GGAAGCAAAT 
GTGCTAATTC AGATCTCTTC TCACGGCGGT TCTCGACGTC AGGAAGCTCA GAGACTGGGC 
AGAGTGCTGC GAGCCAAGAA AGGAATGGTT GCAGAGGAAT ACAATGCTTA CTTCTACTCG 
CTCGTCTCTC AGGACACACA GGAAATGGCC TATTCCACCA AGAGACAGCG CTTCCTGGTG 
GACCAGGGAT ACAGTTTCAA GGTAATCACA AAGTTGGCTG GGATGGAGGA GGAAGACTTA 
ATGTTTTCCA CCCGTGACGA ACAGCAGCAG CTCTTGCAGA AGGTTCTGGC AGCTTCAGAT 
CTTGACGCTG AAGAGGAAGT GGTGATGGGA GAAGTGGGAG GAAAGCCACA GTTTTCTCGT 
CGTGCGGGCA CTATGAGCTC GATGTCTGGA GCAGATGATG CCCTCTACAT GGAATACCAG 
ATGCCCCGTG GCAGCAAAGC CTCGGTCGGC AAGAACATTC ATCCTCTGTT CAAACGCTTC 
CGCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_963876.1
CDS	128..2476
Translation	MGRKDKSDREKKSKKRYYEDEEEDEEVIGGESQEAVPAAAGKQVDESSTKLDEYGAKDYRLQMLLKNDHSSRPLWVAPDGHIFLEAFSPVYKYAQDFLVAISEPVCRPTHAHEYKLTAYSLYAAVSVGLQTSDIIEYLQKLSKTSVPDGIVQFIKLCTVSYGKVKLVLKHNRYFVESAFPDVIQRLLQDTVIRDCRLRSAEGEETELITETISSKSAISKSQQDNGGPSSSQPADGQRSGTQVPEDIFSYYEQMDKEEEEEEETQTVSFEIRQEMIEELQKRCIQLEYPLLAEYDFRNDTVNPDINMDLKPTAVLRPYQEKSLRKMFGNGRARSGVIVLPCGAGKSLVGVTAACTVRKRCLVLGNSSVSVEQWKAQFKMWSTIDDSQICRFTSDAKDKPIGCSVAISTYSMLGHTTKRSWEAERVMEWMKSQEWGLIILDEVHTIPAKMFRRVLTIVQAHCKLGLTATLVREDDKIVDLNFLIGPKLYEANWMELQNNGYIAKVQCAEVWCPMSPEFYREYVAIKTKKRILLYTMNPNKFRACQFLIRFHERRNDKIIVFADNVFALKEYAIRLNKPYIYGPTSQGERMQILQNFKHNPKINTIFISKVGDTSFDLPEANVLIQISSHGGSRRQEAQRLGRVLRAKKGMVAEEYNAYFYSLVSQDTQEMAYSTKRQRFLVDQGYSFKVITKLAGMEEEDLMFSTRDEQQQLLQKVLAASDLDAEEEVVMGEVGGKPQFSRRAGTMSSMSGADDALYMEYQMPRGSKASVGKNIHPLFKRFRK

Target ORF information:

RefSeq Version	NM_201582.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio excision repair cross-complementation group 3 (ercc3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201582.1

ORF Insert Sequence:

ATGGGGAGGA AAGATAAAAG TGACAGAGAG AAGAAGTCCA AGAAACGCTA TTATGAAGAT 
GAAGAGGAGG ATGAAGAGGT GATTGGCGGA GAGTCTCAGG AGGCCGTTCC TGCAGCTGCT 
GGGAAACAGG TGGATGAATC CAGCACTAAA CTGGACGAGT ATGGAGCCAA AGACTACAGG 
CTTCAGATGC TCCTCAAGAA CGACCACTCT TCCCGACCTC TATGGGTGGC TCCTGATGGT 
CACATTTTCC TCGAAGCCTT CTCTCCAGTT TATAAATACG CTCAGGACTT TTTGGTGGCC 
ATTTCAGAGC CGGTGTGTCG ACCCACACAT GCGCATGAGT ACAAACTGAC AGCGTATTCT 
CTGTACGCCG CTGTGAGCGT CGGCCTGCAG ACCTCTGACA TCATTGAGTA CCTGCAGAAA 
CTCAGCAAGA CCTCTGTGCC GGATGGCATA GTGCAGTTTA TTAAGTTGTG TACAGTGAGC 
TATGGTAAAG TCAAACTTGT CCTGAAACAC AACAGATATT TTGTGGAGAG TGCGTTCCCT 
GATGTCATCC AGCGGCTGTT ACAGGACACT GTGATTCGGG ACTGTCGTCT TCGCTCTGCT 
GAGGGTGAGG AGACAGAGCT CATCACCGAG ACCATCTCTA GCAAATCAGC AATCTCAAAG 
TCTCAGCAGG ACAACGGTGG TCCATCGTCC TCTCAGCCTG CAGATGGGCA GCGCTCAGGA 
ACTCAGGTGC CGGAGGACAT CTTCAGTTAC TATGAACAGA TGGATAAAGA GGAGGAGGAG 
GAGGAAGAGA CGCAAACCGT TTCATTTGAG ATCCGTCAGG AAATGATAGA GGAGCTTCAG 
AAGCGCTGTA TTCAGTTGGA GTATCCCCTA CTGGCTGAAT ACGACTTCCG TAATGACACA 
GTGAATCCTG ACATCAACAT GGACCTGAAG CCCACCGCTG TGCTGAGGCC GTATCAGGAA 
AAGAGTCTGA GGAAGATGTT TGGGAATGGC AGAGCTCGAT CTGGAGTCAT CGTCCTGCCT 
TGTGGTGCTG GTAAATCTCT GGTAGGGGTC ACTGCCGCCT GCACGGTGCG CAAACGCTGC 
CTAGTTTTGG GAAACTCCTC TGTGTCAGTG GAACAGTGGA AAGCTCAGTT CAAGATGTGG 
TCCACTATCG ATGACTCCCA GATCTGCCGC TTCACTTCAG ATGCAAAGGA CAAACCCATT 
GGCTGCTCGG TGGCCATCAG CACCTACTCT ATGCTGGGCC ATACCACCAA ACGCTCCTGG 
GAGGCCGAGA GGGTCATGGA GTGGATGAAG AGTCAGGAGT GGGGACTCAT CATACTGGAC 
GAGGTGCACA CCATACCTGC AAAGATGTTC CGTCGTGTTC TTACAATAGT CCAGGCTCAC 
TGTAAACTGG GTCTCACCGC CACTCTGGTA AGAGAAGATG ATAAGATTGT GGATCTGAAC 
TTCCTGATTG GACCAAAGCT CTATGAGGCA AACTGGATGG AACTGCAGAA CAATGGCTAC 
ATCGCTAAAG TCCAGTGTGC TGAGGTGTGG TGTCCCATGT CTCCTGAGTT TTACCGTGAA 
TATGTGGCCA TAAAGACCAA GAAGAGAATT CTGCTCTACA CCATGAATCC CAACAAGTTT 
CGAGCTTGTC AGTTCCTGAT TCGCTTCCAT GAGCGAAGAA ACGACAAGAT CATCGTCTTT 
GCCGACAACG TCTTTGCACT GAAAGAATAC GCCATCCGTC TAAACAAGCC ATATATTTAT 
GGACCCACAT CTCAAGGAGA GCGTATGCAG ATCCTTCAAA ACTTCAAGCA CAATCCCAAA 
ATCAATACGA TCTTCATCTC CAAGGTAGGA GACACTTCAT TTGACCTGCC GGAAGCAAAT 
GTGCTAATTC AGATCTCTTC TCACGGCGGT TCTCGACGTC AGGAAGCTCA GAGACTGGGC 
AGAGTGCTGC GAGCCAAGAA AGGAATGGTT GCAGAGGAAT ACAATGCTTA CTTCTACTCG 
CTCGTCTCTC AGGACACACA GGAAATGGCC TATTCCACCA AGAGACAGCG CTTCCTGGTG 
GACCAGGGAT ACAGTTTCAA GGTAATCACA AAGTTGGCTG GGATGGAGGA GGAAGACTTA 
ATGTTTTCCA CCCGTGACGA ACAGCAGCAG CTCTTGCAGA AGGTTCTGGC AGCTTCAGAT 
CTTGACGCTG AAGAGGAAGT GGTGATGGGA GAAGTGGGAG GAAAGCCACA GTTTTCTCGT 
CGTGCGGGCA CTATGAGCTC GATGTCTGGA GCAGATGATG CCCTCTACAT GGAATACCAG 
ATGCCCCGTG GCAGCAAAGC CTCGGTCGGC AAGAACATTC ATCCTCTGTT CAAACGCTTC 
CGCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Evolutionary conservation of TFIIH subunits: implications for the use of zebrafish as a model to study TFIIH function and regulation.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology172-1739-20(2014 Jun-Jul)
Silva IA,Cox CJ,Leite RB,Cancela ML,Conceição N

Selection of reliable biomarkers from PCR array analyses using relative distance computational model: methodology and proof-of-concept study.
PloS one8(12)e83954(2013)
Liu C,Xu H,Lam SH,Gong Z

Zebrafish reward mutants reveal novel transcripts mediating the behavioral effects of amphetamine.
Genome biology10(7)R81(2009)
Webb KJ,Norton WH,Trümbach D,Meijer AH,Ninkovic J,Topp S,Heck D,Marr C,Wurst W,Theis FJ,Spaink HP,Bally-Cuif L

The zebrafish gene map defines ancestral vertebrate chromosomes.
Genome research15(9)1307-14(2005 Sep)
Woods IG,Wilson C,Friedlander B,Chang P,Reyes DK,Nix R,Kelly PD,Chu F,Postlethwait JH,Talbot WS

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ercc3 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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