trim33 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	trim33
Entrez Gene ID	324053
Full Name	tripartite motif containing 33
Synonyms	cb1085,id:ibd2175,mon,tif1g,wu:fc17f10,zgc:136680
General protein information	Preferred Names tripartite motif containing 33 Names E3 ubiquitin-protein ligase TRIM33 RING-type E3 ubiquitin transferase TRIM33 TIF1-gamma Tif1gamma ectodermin homolog moonshine transcription intermediary factor 1-gamma tripartite motif-containing protein 33 vampire
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005166728.4	XP_005166785.1	E3 ubiquitin-protein ligase TRIM33 isoform X1
XM_005166728.3	XP_005166785.1	E3 ubiquitin-protein ligase TRIM33 isoform X1
NM_001002871.2	NP_001002871.2	E3 ubiquitin-protein ligase TRIM33

Pathways from BioSystems

Homologies

Homo sapiens (human)	TRIM33	NP_148980.2
Macaca mulatta (Rhesus monkey)	TRIM33	XP_002801759.1
Danio rerio (zebrafish)	trim33	NP_001002871.2
Pan troglodytes (chimpanzee)	TRIM33	XP_003308352.1
Rattus norvegicus (Norway rat)	Trim33	XP_006233162.1
Bos taurus (cattle)	TRIM33	NP_001192947.1
Mus musculus (house mouse)	Trim33	NP_444400.2
Canis lupus familiaris (dog)	TRIM33	XP_533013.3
Gallus gallus (chicken)	TRIM33	XP_418009.4

Gene Ontology
Bibliography

Related articles in PubMed

Soybean Meal-Induced Intestinal Inflammation in Zebrafish Is T Cell-Dependent and Has a Th17 Cytokine Profile.
Coronado M, Solis CJ, Hernandez PP, Feijóo CG
Frontiers in immunology10610(2019)

Trim33 is essential for macrophage and neutrophil mobilization to developmental or inflammatory cues.
Demy DL, Tauzin M, Lancino M, Le Cabec V, Redd M, Murayama E, Maridonneau-Parini I, Trede N, Herbomel P
Journal of cell science130(17)2797-2807(2017 Sep)

Transforming Growth Factor β Drives Hemogenic Endothelium Programming and the Transition to Hematopoietic Stem Cells.
Monteiro R, Pinheiro P, Joseph N, Peterkin T, Koth J, Repapi E, Bonkhofer F, Kirmizitas A, Patient R
Developmental cell38(4)358-70(2016 08)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Genome-wide analysis of the zebrafish Klf family identifies two genes important for erythroid maturation.
Xue Y, Gao S, Liu F
Developmental biology403(2)115-27(2015 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following trim33 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the trim33 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa02993	NM_001002871.2 Latest version!	Danio rerio tripartite motif containing 33 (trim33), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa13905	XM_005166728.4 Latest version!	Danio rerio tripartite motif containing 33 (trim33), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
ODa13905	XM_005166728.3	Danio rerio tripartite motif containing 33 (trim33), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

trim33 ( NM_001002871.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001002871.2 Danio rerio tripartite motif containing 33 (trim33), mRNA.

Price & Availability↑

CloneID	ODa02993
Clone ID Related Accession (Same CDS sequence)	NM_001002871.2
Accession Version	NM_001002871.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569513600000
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase TRIM33
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX321886.14. On Sep 17, 2011 this sequence version replaced NM_001002871.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GFIL01041948.1, AY598453.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGACA ACAAAGGCCA AGATTGTACG GAGAGCTCCG CAAATATGGA GGTAGAGGCG 
TCGGGGACGG AAGAAAACGG AGACGGTGGC GCAAAAGAGC CTGAGGCGGT TGTTTCAATC 
GACACGGAGC CGAAAGAAGC AGCAGACGAG GCCAAATCTC AAGAGACCGT TCCCCAGGCG 
CCGACGACAT CCAGCGATAG CAGCAGCAGT GGCGGCGGCG GCGAAGCTAC AGACGGGACC 
CCGAATGCTG AAAGCGCGGA TCCTCCTCCT CCACCACCAC CACCTCCGCC GCCGCCACCG 
TCCACCCCAG CGGACAGCAC CGCAGCTGCC GCATCACCCG CTGTGGTTAC CGAGATGTCG 
CCTCCTCCAC CAGCATCCAC ATCTTCATCA TCTTCCACCC CTGCCGCCCC GATAAACCTG 
CTGGATACGT GCGCTGTGTG TAAACAGAGT CTCCAGAACC GCGACTGTGA ACCCAAACTC 
CTGCCCTGCT TGCACTCCTT TTGCCTCAAG TGCATCCCAC AGCCCGATCG TAAGATCACC 
ATGCCGGTGC AAGGCCCCCA CGGACAGGAC ACTCGAATCG TGAACGTCAT GCGTTGTACG 
GTGTGCCATC AGGACTACAA ACAGATTGAC ATGGTGGACA ATTACTTCGT CAAGGACACT 
TCAGAGGCCA CAAACACGTC AGTGGAGAAC TCGACACAGG TGTGCACCAG CTGTGAAGAT 
AATGCCAGTG CTATTGGATT CTGCGTTGAA TGTGGAGAGT GGCTCTGCAA AACTTGCATC 
GAAGCGCACC AAAGGGTCAA ATTTACCAAG GACCATAAAA TACGCAAAAA AGAGGAAGTG 
TCTCCTGAGT CTGTTGGGAC ATCAGGCCAG AGACCAGTTT TCTGTCCGGT ACACAAACAA 
GAGGCCCTCA AGCTCTTCTG TGAGACCTGC GACACTCTGA CCTGCAGAGA CTGCCAGCTA 
CTTGAACACA AAGAACACAG GTACCAGTTT CTAGATGAAG CCTGTCAAAA CCAGAAAGGC 
ATCATTGCGA CATTCATGAC TAAGTTACAG GAGAAGAGAG GCTTAGTGGA GTACTCTGCA 
TCTGAAGTGC AAAAAAGACT CAAAGAAGTT GCTGAGACGC ATAAGAAGGT GGAACATGAG 
ATAAAGATAG CAGTCTTTAC TCTTATCAAT GAAATCAACA AGAAGGGGAA ATCTCTGCTG 
CAGCAGTTGG AGAGTGTGAC CAAAGACAGG AGCATGAAGC TGCTGAGCCA ACAGAGGGAC 
ATCAGTGTTC TGGCCCAGCA GATCATCCAT GTGCTCAACT TCACCAACTG GGCCATCACT 
AACGGCAGCA GCACTGCCCT GCTCTACAGC AAGAGACTGA TCACGTACCA GCTGCGTCTG 
ATTATGAAGG CCCGTGTGGA TGCAGTACCT CCAGCTAATG GAGCAGTTCG CTTCTTCTGT 
GACCCAACCT TTTGGGCCAA GAATGTGGTT AACCTTGGCA ATCTAGTCAT TGAAAAAGTT 
GCTCCTACTG CTCCTCCGTC CAACATGATG GTCAATCAGC AGATCTCTCC CGGTCACAAC 
CACCCAGGTA AGCACTCAGG ACAGATCAAC CTGGCCCAGC TGAGGCTGCA GCACATGCAG 
CAGGCAGCGA TTGCTCAGAA ACATCAGCAG CAACACCAAC ACCACCAACA ACAACAACAC 
CAACACCAAC AACAGCAGCA GCAACAACAA CAACAACAAC AACAACAGCA GCAGCAACAA 
CAACAACAGC AACACCAGCA ACAAATTCAG CAGCAGATGC GCATCGCCTC TCAGATGTCC 
CAGCATCCCA GACAAGGAGC TCCACAAATG ATTCAGCAAC CTCCTCCGAG ACTCATTAGT 
ATGCAGGCCC TCCACAGGGG CGGAGTTAAT GGTTCCTCCC ACATGTTTCC GCCTCATCAC 
CTGCGCATGG TGTCAGCGCA GAACCGCATG CCTTCTGCTC AACCTCGACT CAATGGCCAG 
CCATATCCCA TGATGCAACC TCAACTTCAG AGACAGCACT CTAATCCTGG GCATGCGGGT 
CCATTCCCTG TGGCGTCGCT ACACAACATT AGTGCTGCCA ACCCCACCAG TCCCACCAGT 
GCCAGTATGG CCAATGCCCA TATGCACCGC GGCCCCTCCA GTCCTGTTAT CACCCCTATA 
GAGCTCATAC CGTCAGTCAC CAACCCTGAG AACCTGCCCT GCCTACCCGA CATCCCTCCT 
ATTCAGCTTG AAGATGCCGG CTCTAGCACA CTGGATAATA TCCTAAGTCG GTATATTTCT 
GCTAATGCAT ATCCTACTGT CGGACCCACA AACCCATCGC CAGGACCTTC CACACATTCT 
CCGGGATCGT CAGGTTTATC TAATTCTCAC ACTCCAGCTC GTCCATCCAG CACGTCAAGT 
ACAGGCAGTC GGGGCAGTTC TGGAACCACA GTGGACCAGG TGAAGGTCAA ACAGGAGCCA 
GGAGTTGAGG AAGAATGTAG CTATACTGGA GCAAACGTCA AAACTGAACG AACTAAAGAT 
GGCAGACGGA GCGCTTGTAT GATGAGCAGT CCAGAAGGAA GTTTAACTCC CCCTCTCCCC 
ATCCTTGGCT CCGTGTCCAC CGGCTCAGTC CAGGACATTT TAAGGACACT TGGTGAAAAC 
GTTAAATCTG AACCACAGAG CGATAACCTT TCCTCCTGCA CGAACCCGAA CTCCAGAGCA 
ACTCTAACCA ATGGGACCAG CGGGTCGAAT GGAGGCCAGC GAGGCGGCAC TACTAATGCT 
AACAGTCAGA CCACTGCGGG AAAGGAAGAC GACCCGAATG AAGACTGGTG TGCTGTTTGT 
CAGAACGGAG GAGAACTGCT GTGCTGCGAT CACTGTCCCA AAGTCTTCCA CATAACCTGT 
CATATCCCAA CACTCAAATC CTCTCCCAGT GGGGATTGGA TGTGCACGTT CTGCAGAAAT 
CTCGCAAATC CAGAGATCGA GTACAACTGT GATGACGATC CACCCAGAAA CAAAGAAAAA 
AATGAGATGG CAATGAGTCC AGAAGAACAA AGGCGATGTG AGCGTCTGCT GCTGCATGTT 
TTCTGTCATG AGCTCAGCAC AGAGTTTCAA GAGCCCGTTC CTACCTCGGT TCCTAATTAC 
TATAAAATAA TAAAGCATCC GATGGATTTG ACCCTGGTGA AGCGGAAACT ACAGAGAAAG 
CATCCTCTGC ATTACAAGAG CCCTAAAGAG TTTGTGTCGG ACGTGCGCCT TGTCTTTAGC 
AATTGTGCCA AATATAATGA GATGTCTCGA ATAATCCAGG TTTATGATGA GGAGAAACAG 
AGTAATGTCC AGGCCGATTC GGAAGTGGCA GAAGCTGGAA AAGCCGTGAG TCTGTACTTT 
GAGGAGCGAT TGCTGGAGAT TTTTCCAGAG CAGACGTTCC CGGTGGTGAT GGAGAAGGAA 
ACTCAAATCG AGGCCGAAAA GGAGGATTCG GACGACTCCG ATGATGACAT CATACAACCC 
AAACGCAAAC GCTTAAAAGT GGACACAGAG ATGCTGCTGC ACATCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001002871.2
CDS	14..3544
Translation	MADNKGQDCTESSANMEVEASGTEENGDGGAKEPEAVVSIDTEPKEAADEAKSQETVPQAPTTSSDSSSSGGGGEATDGTPNAESADPPPPPPPPPPPPPSTPADSTAAAASPAVVTEMSPPPPASTSSSSSTPAAPINLLDTCAVCKQSLQNRDCEPKLLPCLHSFCLKCIPQPDRKITMPVQGPHGQDTRIVNVMRCTVCHQDYKQIDMVDNYFVKDTSEATNTSVENSTQVCTSCEDNASAIGFCVECGEWLCKTCIEAHQRVKFTKDHKIRKKEEVSPESVGTSGQRPVFCPVHKQEALKLFCETCDTLTCRDCQLLEHKEHRYQFLDEACQNQKGIIATFMTKLQEKRGLVEYSASEVQKRLKEVAETHKKVEHEIKIAVFTLINEINKKGKSLLQQLESVTKDRSMKLLSQQRDISVLAQQIIHVLNFTNWAITNGSSTALLYSKRLITYQLRLIMKARVDAVPPANGAVRFFCDPTFWAKNVVNLGNLVIEKVAPTAPPSNMMVNQQISPGHNHPGKHSGQINLAQLRLQHMQQAAIAQKHQQQHQHHQQQQHQHQQQQQQQQQQQQQQQQQQQQQQHQQQIQQQMRIASQMSQHPRQGAPQMIQQPPPRLISMQALHRGGVNGSSHMFPPHHLRMVSAQNRMPSAQPRLNGQPYPMMQPQLQRQHSNPGHAGPFPVASLHNISAANPTSPTSASMANAHMHRGPSSPVITPIELIPSVTNPENLPCLPDIPPIQLEDAGSSTLDNILSRYISANAYPTVGPTNPSPGPSTHSPGSSGLSNSHTPARPSSTSSTGSRGSSGTTVDQVKVKQEPGVEEECSYTGANVKTERTKDGRRSACMMSSPEGSLTPPLPILGSVSTGSVQDILRTLGENVKSEPQSDNLSSCTNPNSRATLTNGTSGSNGGQRGGTTNANSQTTAGKEDDPNEDWCAVCQNGGELLCCDHCPKVFHITCHIPTLKSSPSGDWMCTFCRNLANPEIEYNCDDDPPRNKEKNEMAMSPEEQRRCERLLLHVFCHELSTEFQEPVPTSVPNYYKIIKHPMDLTLVKRKLQRKHPLHYKSPKEFVSDVRLVFSNCAKYNEMSRIIQVYDEEKQSNVQADSEVAEAGKAVSLYFEERLLEIFPEQTFPVVMEKETQIEAEKEDSDDSDDDIIQPKRKRLKVDTEMLLHIK

Target ORF information:

RefSeq Version	NM_001002871.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tripartite motif containing 33 (trim33), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001002871.2

ORF Insert Sequence:

ATGGCGGACA ACAAAGGCCA AGATTGTACG GAGAGCTCCG CAAATATGGA GGTAGAGGCG 
TCGGGGACGG AAGAAAACGG AGACGGTGGC GCAAAAGAGC CTGAGGCGGT TGTTTCAATC 
GACACGGAGC CGAAAGAAGC AGCAGACGAG GCCAAATCTC AAGAGACCGT TCCCCAGGCG 
CCGACGACAT CCAGCGATAG CAGCAGCAGT GGCGGCGGCG GCGAAGCTAC AGACGGGACC 
CCGAATGCTG AAAGCGCGGA TCCTCCTCCT CCACCACCAC CACCTCCGCC GCCGCCACCG 
TCCACCCCAG CGGACAGCAC CGCAGCTGCC GCATCACCCG CTGTGGTTAC CGAGATGTCG 
CCTCCTCCAC CAGCATCCAC ATCTTCATCA TCTTCCACCC CTGCCGCCCC GATAAACCTG 
CTGGATACGT GCGCTGTGTG TAAACAGAGT CTCCAGAACC GCGACTGTGA ACCCAAACTC 
CTGCCCTGCT TGCACTCCTT TTGCCTCAAG TGCATCCCAC AGCCCGATCG TAAGATCACC 
ATGCCGGTGC AAGGCCCCCA CGGACAGGAC ACTCGAATCG TGAACGTCAT GCGTTGTACG 
GTGTGCCATC AGGACTACAA ACAGATTGAC ATGGTGGACA ATTACTTCGT CAAGGACACT 
TCAGAGGCCA CAAACACGTC AGTGGAGAAC TCGACACAGG TGTGCACCAG CTGTGAAGAT 
AATGCCAGTG CTATTGGATT CTGCGTTGAA TGTGGAGAGT GGCTCTGCAA AACTTGCATC 
GAAGCGCACC AAAGGGTCAA ATTTACCAAG GACCATAAAA TACGCAAAAA AGAGGAAGTG 
TCTCCTGAGT CTGTTGGGAC ATCAGGCCAG AGACCAGTTT TCTGTCCGGT ACACAAACAA 
GAGGCCCTCA AGCTCTTCTG TGAGACCTGC GACACTCTGA CCTGCAGAGA CTGCCAGCTA 
CTTGAACACA AAGAACACAG GTACCAGTTT CTAGATGAAG CCTGTCAAAA CCAGAAAGGC 
ATCATTGCGA CATTCATGAC TAAGTTACAG GAGAAGAGAG GCTTAGTGGA GTACTCTGCA 
TCTGAAGTGC AAAAAAGACT CAAAGAAGTT GCTGAGACGC ATAAGAAGGT GGAACATGAG 
ATAAAGATAG CAGTCTTTAC TCTTATCAAT GAAATCAACA AGAAGGGGAA ATCTCTGCTG 
CAGCAGTTGG AGAGTGTGAC CAAAGACAGG AGCATGAAGC TGCTGAGCCA ACAGAGGGAC 
ATCAGTGTTC TGGCCCAGCA GATCATCCAT GTGCTCAACT TCACCAACTG GGCCATCACT 
AACGGCAGCA GCACTGCCCT GCTCTACAGC AAGAGACTGA TCACGTACCA GCTGCGTCTG 
ATTATGAAGG CCCGTGTGGA TGCAGTACCT CCAGCTAATG GAGCAGTTCG CTTCTTCTGT 
GACCCAACCT TTTGGGCCAA GAATGTGGTT AACCTTGGCA ATCTAGTCAT TGAAAAAGTT 
GCTCCTACTG CTCCTCCGTC CAACATGATG GTCAATCAGC AGATCTCTCC CGGTCACAAC 
CACCCAGGTA AGCACTCAGG ACAGATCAAC CTGGCCCAGC TGAGGCTGCA GCACATGCAG 
CAGGCAGCGA TTGCTCAGAA ACATCAGCAG CAACACCAAC ACCACCAACA ACAACAACAC 
CAACACCAAC AACAGCAGCA GCAACAACAA CAACAACAAC AACAACAGCA GCAGCAACAA 
CAACAACAGC AACACCAGCA ACAAATTCAG CAGCAGATGC GCATCGCCTC TCAGATGTCC 
CAGCATCCCA GACAAGGAGC TCCACAAATG ATTCAGCAAC CTCCTCCGAG ACTCATTAGT 
ATGCAGGCCC TCCACAGGGG CGGAGTTAAT GGTTCCTCCC ACATGTTTCC GCCTCATCAC 
CTGCGCATGG TGTCAGCGCA GAACCGCATG CCTTCTGCTC AACCTCGACT CAATGGCCAG 
CCATATCCCA TGATGCAACC TCAACTTCAG AGACAGCACT CTAATCCTGG GCATGCGGGT 
CCATTCCCTG TGGCGTCGCT ACACAACATT AGTGCTGCCA ACCCCACCAG TCCCACCAGT 
GCCAGTATGG CCAATGCCCA TATGCACCGC GGCCCCTCCA GTCCTGTTAT CACCCCTATA 
GAGCTCATAC CGTCAGTCAC CAACCCTGAG AACCTGCCCT GCCTACCCGA CATCCCTCCT 
ATTCAGCTTG AAGATGCCGG CTCTAGCACA CTGGATAATA TCCTAAGTCG GTATATTTCT 
GCTAATGCAT ATCCTACTGT CGGACCCACA AACCCATCGC CAGGACCTTC CACACATTCT 
CCGGGATCGT CAGGTTTATC TAATTCTCAC ACTCCAGCTC GTCCATCCAG CACGTCAAGT 
ACAGGCAGTC GGGGCAGTTC TGGAACCACA GTGGACCAGG TGAAGGTCAA ACAGGAGCCA 
GGAGTTGAGG AAGAATGTAG CTATACTGGA GCAAACGTCA AAACTGAACG AACTAAAGAT 
GGCAGACGGA GCGCTTGTAT GATGAGCAGT CCAGAAGGAA GTTTAACTCC CCCTCTCCCC 
ATCCTTGGCT CCGTGTCCAC CGGCTCAGTC CAGGACATTT TAAGGACACT TGGTGAAAAC 
GTTAAATCTG AACCACAGAG CGATAACCTT TCCTCCTGCA CGAACCCGAA CTCCAGAGCA 
ACTCTAACCA ATGGGACCAG CGGGTCGAAT GGAGGCCAGC GAGGCGGCAC TACTAATGCT 
AACAGTCAGA CCACTGCGGG AAAGGAAGAC GACCCGAATG AAGACTGGTG TGCTGTTTGT 
CAGAACGGAG GAGAACTGCT GTGCTGCGAT CACTGTCCCA AAGTCTTCCA CATAACCTGT 
CATATCCCAA CACTCAAATC CTCTCCCAGT GGGGATTGGA TGTGCACGTT CTGCAGAAAT 
CTCGCAAATC CAGAGATCGA GTACAACTGT GATGACGATC CACCCAGAAA CAAAGAAAAA 
AATGAGATGG CAATGAGTCC AGAAGAACAA AGGCGATGTG AGCGTCTGCT GCTGCATGTT 
TTCTGTCATG AGCTCAGCAC AGAGTTTCAA GAGCCCGTTC CTACCTCGGT TCCTAATTAC 
TATAAAATAA TAAAGCATCC GATGGATTTG ACCCTGGTGA AGCGGAAACT ACAGAGAAAG 
CATCCTCTGC ATTACAAGAG CCCTAAAGAG TTTGTGTCGG ACGTGCGCCT TGTCTTTAGC 
AATTGTGCCA AATATAATGA GATGTCTCGA ATAATCCAGG TTTATGATGA GGAGAAACAG 
AGTAATGTCC AGGCCGATTC GGAAGTGGCA GAAGCTGGAA AAGCCGTGAG TCTGTACTTT 
GAGGAGCGAT TGCTGGAGAT TTTTCCAGAG CAGACGTTCC CGGTGGTGAT GGAGAAGGAA 
ACTCAAATCG AGGCCGAAAA GGAGGATTCG GACGACTCCG ATGATGACAT CATACAACCC 
AAACGCAAAC GCTTAAAAGT GGACACAGAG ATGCTGCTGC ACATCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trim33 ( XM_005166728.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166785.1 Danio rerio tripartite motif containing 33 (trim33), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa13905
Clone ID Related Accession (Same CDS sequence)	XM_005166728.4 , XM_005166728.3
Accession Version	XM_005166728.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase TRIM33 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005166728.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACA ACAAAGGCCA AGATTGTACG GAGAGCTCCG CAAATATGGA GGTAGAGGCG 
TCGGGGACGG AAGAAAACGG AGACGGTGGC GCAAAAGAGC CTGAGGCGGT TGTTTCAATC 
GACACGGAGC CGAAAGAAGC AGCAGACGAG GCCAAATCTC AAGAGACCGT TCCCCAGGCG 
CCGACGACAT CCAGCGATAG CAGCAGCAGT GGCGGCGGCG GCGAAGCTAC AGACGGGACC 
CCGAATGCTG AAAGCGCGGA TCCTCCTCCT CCACCACCAC CACCTCCGCC GCCGCCACCG 
TCCACCCCAG CGGACAGCAC CGCAGCTGCC GCATCACCCG CTGTGGTTAC CGAGATGTCG 
CCTCCTCCAC CAGCATCCAC ATCTTCATCA TCTTCCACCC CTGCCGCCCC GATAAACCTG 
CTGGATACGT GCGCTGTGTG TAAACAGAGT CTCCAGAACC GCGACTGTGA ACCCAAACTC 
CTGCCCTGCT TGCACTCCTT TTGCCTCAAG TGCATCCCAC AGCCCGATCG TAAGATCACC 
ATGCCGGTGC AAGGCCCCCA CGGACAGGAC ACTCGAATCG TGAACGTCAT GCGTTGTACG 
GTGTGCCATC AGGACTACAA ACAGATTGAC ATGGTGGACA ATTACTTCGT CAAGGACACT 
TCAGAGGCCA CAAACACGTC AGTGGAGAAC TCGACACAGG TGTGCACCAG CTGTGAAGAT 
AATGCCAGTG CTATTGGATT CTGCGTTGAA TGTGGAGAGT GGCTCTGCAA AACTTGCATC 
GAAGCGCACC AAAGGGTCAA ATTTACCAAG GACCATAAAA TACGCAAAAA AGAGGAAGTG 
TCTCCTGAGT CTGTTGGGAC ATCAGGCCAG AGACCAGTTT TCTGTCCGGT ACACAAACAA 
GAGGCCCTCA AGCTCTTCTG TGAGACCTGC GACACTCTGA CCTGCAGAGA CTGCCAGCTA 
CTTGAACACA AAGAACACAG GTACCAGTTT CTAGATGAAG CCTGTCAAAA CCAGAAAGGC 
ATCATTGCGA CATTCATGAC TAAGTTACAG GAGAAGAGAG GCTTAGTGGA GTACTCTGCA 
TCTGAAGTGC AAAAAAGACT CAAAGAAGTT GCTGAGACGC ATAAGAAGGT GGAACATGAG 
ATAAAGATAG CAGTCTTTAC TCTTATCAAT GAAATCAACA AGAAGGGGAA ATCTCTGCTG 
CAGCAGTTGG AGAGTGTGAC CAAAGACAGG AGCATGAAGC TGCTGAGCCA ACAGAGGGAC 
ATCAGTGTTC TGGCCCAGCA GATCATCCAT GTGCTCAACT TCACCAACTG GGCCATCACT 
AACGGCAGCA GCACTGCCCT GCTCTACAGC AAGAGACTGA TCACGTACCA GCTGCGTCTG 
ATTATGAAGG CCCGTGTGGA TGCAGTACCT CCAGCTAATG GAGCAGTTCG CTTCTTCTGT 
GACCCAACCT TTTGGGCCAA GAATGTGGTT AACCTTGGCA ATCTAGTCAT TGAAAAAGTT 
GCTCCTACTG CTCCTCCGTC CAACATGATG GTCAATCAGC AGATCTCTCC CGGTCACAAC 
CACCCAGGTA AGCACTCAGG ACAGATCAAC CTGGCCCAGC TGAGGCTGCA GCACATGCAG 
CAGGCAGCGA TTGCTCAGAA ACATCAGCAG CAACACCAAC ACCACCAACA ACAACAACAC 
CAACACCAAC AACAGCAGCA GCAACAACAA CAACAACAAC AACAACAGCA GCAGCAACAA 
CAACAACAGC AACACCAGCA ACAAATTCAG CAGCAGATGC GCATCGCCTC TCAGATGTCC 
CAGCATCCCA GACAAGGAGC TCCACAAATG ATTCAGCAAC CTCCTCCGAG ACTCATTAGT 
ATGCAGGCCC TCCACAGGGG CGGAGTTAAT GGTTCCTCCC ACATGTTTCC GCCTCATCAC 
CTGCGCATGG TGTCAGCGCA GAACCGCATG CCTTCTGCTC AACCTCGACT CAATGGCCAG 
CCATATCCCA TGATGCAACC TCAACTTCAG AGACAGCACT CTAATCCTGG GCATGCGGGT 
CCATTCCCTG TGGCGTCGCT ACACAACATT AGTGCTGCCA ACCCCACCAG TCCCACCAGT 
GCCAGTATGG CCAATGCCCA TATGCACCGC GGCCCCTCCA GTCCTGTTAT CACCCCTATA 
GAGCTCATAC CGTCAGTCAC CAACCCTGAG AACCTGCCCT GCCTACCCGA CATCCCTCCT 
ATTCAGCTTG AAGATGCCGG CTCTAGCACA CTGGATAATA TCCTAAGTCG GTATATTTCT 
GCTAATGCAT ATCCTACTGT CGGACCCACA AACCCATCGC CAGGACCTTC CACACATTCT 
CCGGGATCGT CAGGTTTATC TAATTCTCAC ACTCCAGCTC GTCCATCCAG CACGTCAAGT 
ACAGGCAGTC GGGGCAGTTC TGGAACCACA GTGGACCAGG TGAAGGTCAA ACAGGAGCCA 
GGAGTTGAGG AAGAATGTAG CTATACTGGA GCAAACGTCA AAACTGAACG AACTAAAGAT 
GGCAGACGGA GCGCTTGTAT GATGAGCAGT CCAGAAGGAA GTTTAACTCC CCCTCTCCCC 
ATCCTTGGCT CCGTGTCCAC CGGCTCAGTC CAGGACATTT TAAGGACACT TGGTGAAAAC 
GTTAAATCTG AACCACAGAG CGATAACCTT TCCTCCTGCA CGAACCCGAA CTCCAGAGCA 
ACTCTAACCA ATGGGACCAG CGGGTCGAAT GGAGGCCAGC GAGGCGGCAC TACTAATGCT 
AACAGTCAGA CCACTGCGGG AAAGGAAGAC GACCCGAATG AAGACTGGTG TGCTGTTTGT 
CAGAACGGAG GAGAACTGCT GTGCTGCGAT CACTGTCCCA AAGTCTTCCA CATAACCTGT 
CATATCCCAA CACTCAAATC CTCTCCCAGT GGGGATTGGA TGTGCACGTT CTGCAGAAAT 
CTCGCAAATC CAGAGATCGA GTACAACTGT GATGACGATC CACCCAGAAA CAAAGAAAAA 
AATGAGATGG CAATGAGTCC AGAAGAACAA AGGCGATGTG AGCGTCTGCT GCTGCATGTT 
TTCTGTCATG AGCTCAGCAC AGAGTTTCAA GAGCCCGTTC CTACCTCGGT TCCTAATTAC 
TATAAAATAA TAAAGCATCC GATGGATTTG ACCCTGGTGA AGCGGAAACT ACAGAGAAAG 
CATCCTCTGC ATTACAAGAG CCCTAAAGAG TTTGTGTCGG ACGTGCGCCT TGTCTTTAGC 
AATTGTGCCA AATATAATGA GGCCGATTCG GAAGTGGCAG AAGCTGGAAA AGCCGTGAGT 
CTGTACTTTG AGGAGCGATT GCTGGAGATT TTTCCAGAGC AGACGTTCCC GGTGGTGATG 
GAGAAGGAAA CTCAAATCGA GGCCGAAAAG GAGGATTCGG ACGACTCCGA TGATGACATC 
ATACAACCCA AACGCAAACG CTTAAAAGTG GACACAGAGA TGCTGCTGCA CATCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166785.1
CDS	131..3610
Translation	MADNKGQDCTESSANMEVEASGTEENGDGGAKEPEAVVSIDTEPKEAADEAKSQETVPQAPTTSSDSSSSGGGGEATDGTPNAESADPPPPPPPPPPPPPSTPADSTAAAASPAVVTEMSPPPPASTSSSSSTPAAPINLLDTCAVCKQSLQNRDCEPKLLPCLHSFCLKCIPQPDRKITMPVQGPHGQDTRIVNVMRCTVCHQDYKQIDMVDNYFVKDTSEATNTSVENSTQVCTSCEDNASAIGFCVECGEWLCKTCIEAHQRVKFTKDHKIRKKEEVSPESVGTSGQRPVFCPVHKQEALKLFCETCDTLTCRDCQLLEHKEHRYQFLDEACQNQKGIIATFMTKLQEKRGLVEYSASEVQKRLKEVAETHKKVEHEIKIAVFTLINEINKKGKSLLQQLESVTKDRSMKLLSQQRDISVLAQQIIHVLNFTNWAITNGSSTALLYSKRLITYQLRLIMKARVDAVPPANGAVRFFCDPTFWAKNVVNLGNLVIEKVAPTAPPSNMMVNQQISPGHNHPGKHSGQINLAQLRLQHMQQAAIAQKHQQQHQHHQQQQHQHQQQQQQQQQQQQQQQQQQQQQQHQQQIQQQMRIASQMSQHPRQGAPQMIQQPPPRLISMQALHRGGVNGSSHMFPPHHLRMVSAQNRMPSAQPRLNGQPYPMMQPQLQRQHSNPGHAGPFPVASLHNISAANPTSPTSASMANAHMHRGPSSPVITPIELIPSVTNPENLPCLPDIPPIQLEDAGSSTLDNILSRYISANAYPTVGPTNPSPGPSTHSPGSSGLSNSHTPARPSSTSSTGSRGSSGTTVDQVKVKQEPGVEEECSYTGANVKTERTKDGRRSACMMSSPEGSLTPPLPILGSVSTGSVQDILRTLGENVKSEPQSDNLSSCTNPNSRATLTNGTSGSNGGQRGGTTNANSQTTAGKEDDPNEDWCAVCQNGGELLCCDHCPKVFHITCHIPTLKSSPSGDWMCTFCRNLANPEIEYNCDDDPPRNKEKNEMAMSPEEQRRCERLLLHVFCHELSTEFQEPVPTSVPNYYKIIKHPMDLTLVKRKLQRKHPLHYKSPKEFVSDVRLVFSNCAKYNEADSEVAEAGKAVSLYFEERLLEIFPEQTFPVVMEKETQIEAEKEDSDDSDDDIIQPKRKRLKVDTEMLLHIK

Target ORF information:

RefSeq Version	XM_005166728.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tripartite motif containing 33 (trim33), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166728.4

ORF Insert Sequence:

ATGGCGGACA ACAAAGGCCA AGATTGTACG GAGAGCTCCG CAAATATGGA GGTAGAGGCG 
TCGGGGACGG AAGAAAACGG AGACGGTGGC GCAAAAGAGC CTGAGGCGGT TGTTTCAATC 
GACACGGAGC CGAAAGAAGC AGCAGACGAG GCCAAATCTC AAGAGACCGT TCCCCAGGCG 
CCGACGACAT CCAGCGATAG CAGCAGCAGT GGCGGCGGCG GCGAAGCTAC AGACGGGACC 
CCGAATGCTG AAAGCGCGGA TCCTCCTCCT CCACCACCAC CACCTCCGCC GCCGCCACCG 
TCCACCCCAG CGGACAGCAC CGCAGCTGCC GCATCACCCG CTGTGGTTAC CGAGATGTCG 
CCTCCTCCAC CAGCATCCAC ATCTTCATCA TCTTCCACCC CTGCCGCCCC GATAAACCTG 
CTGGATACGT GCGCTGTGTG TAAACAGAGT CTCCAGAACC GCGACTGTGA ACCCAAACTC 
CTGCCCTGCT TGCACTCCTT TTGCCTCAAG TGCATCCCAC AGCCCGATCG TAAGATCACC 
ATGCCGGTGC AAGGCCCCCA CGGACAGGAC ACTCGAATCG TGAACGTCAT GCGTTGTACG 
GTGTGCCATC AGGACTACAA ACAGATTGAC ATGGTGGACA ATTACTTCGT CAAGGACACT 
TCAGAGGCCA CAAACACGTC AGTGGAGAAC TCGACACAGG TGTGCACCAG CTGTGAAGAT 
AATGCCAGTG CTATTGGATT CTGCGTTGAA TGTGGAGAGT GGCTCTGCAA AACTTGCATC 
GAAGCGCACC AAAGGGTCAA ATTTACCAAG GACCATAAAA TACGCAAAAA AGAGGAAGTG 
TCTCCTGAGT CTGTTGGGAC ATCAGGCCAG AGACCAGTTT TCTGTCCGGT ACACAAACAA 
GAGGCCCTCA AGCTCTTCTG TGAGACCTGC GACACTCTGA CCTGCAGAGA CTGCCAGCTA 
CTTGAACACA AAGAACACAG GTACCAGTTT CTAGATGAAG CCTGTCAAAA CCAGAAAGGC 
ATCATTGCGA CATTCATGAC TAAGTTACAG GAGAAGAGAG GCTTAGTGGA GTACTCTGCA 
TCTGAAGTGC AAAAAAGACT CAAAGAAGTT GCTGAGACGC ATAAGAAGGT GGAACATGAG 
ATAAAGATAG CAGTCTTTAC TCTTATCAAT GAAATCAACA AGAAGGGGAA ATCTCTGCTG 
CAGCAGTTGG AGAGTGTGAC CAAAGACAGG AGCATGAAGC TGCTGAGCCA ACAGAGGGAC 
ATCAGTGTTC TGGCCCAGCA GATCATCCAT GTGCTCAACT TCACCAACTG GGCCATCACT 
AACGGCAGCA GCACTGCCCT GCTCTACAGC AAGAGACTGA TCACGTACCA GCTGCGTCTG 
ATTATGAAGG CCCGTGTGGA TGCAGTACCT CCAGCTAATG GAGCAGTTCG CTTCTTCTGT 
GACCCAACCT TTTGGGCCAA GAATGTGGTT AACCTTGGCA ATCTAGTCAT TGAAAAAGTT 
GCTCCTACTG CTCCTCCGTC CAACATGATG GTCAATCAGC AGATCTCTCC CGGTCACAAC 
CACCCAGGTA AGCACTCAGG ACAGATCAAC CTGGCCCAGC TGAGGCTGCA GCACATGCAG 
CAGGCAGCGA TTGCTCAGAA ACATCAGCAG CAACACCAAC ACCACCAACA ACAACAACAC 
CAACACCAAC AACAGCAGCA GCAACAACAA CAACAACAAC AACAACAGCA GCAGCAACAA 
CAACAACAGC AACACCAGCA ACAAATTCAG CAGCAGATGC GCATCGCCTC TCAGATGTCC 
CAGCATCCCA GACAAGGAGC TCCACAAATG ATTCAGCAAC CTCCTCCGAG ACTCATTAGT 
ATGCAGGCCC TCCACAGGGG CGGAGTTAAT GGTTCCTCCC ACATGTTTCC GCCTCATCAC 
CTGCGCATGG TGTCAGCGCA GAACCGCATG CCTTCTGCTC AACCTCGACT CAATGGCCAG 
CCATATCCCA TGATGCAACC TCAACTTCAG AGACAGCACT CTAATCCTGG GCATGCGGGT 
CCATTCCCTG TGGCGTCGCT ACACAACATT AGTGCTGCCA ACCCCACCAG TCCCACCAGT 
GCCAGTATGG CCAATGCCCA TATGCACCGC GGCCCCTCCA GTCCTGTTAT CACCCCTATA 
GAGCTCATAC CGTCAGTCAC CAACCCTGAG AACCTGCCCT GCCTACCCGA CATCCCTCCT 
ATTCAGCTTG AAGATGCCGG CTCTAGCACA CTGGATAATA TCCTAAGTCG GTATATTTCT 
GCTAATGCAT ATCCTACTGT CGGACCCACA AACCCATCGC CAGGACCTTC CACACATTCT 
CCGGGATCGT CAGGTTTATC TAATTCTCAC ACTCCAGCTC GTCCATCCAG CACGTCAAGT 
ACAGGCAGTC GGGGCAGTTC TGGAACCACA GTGGACCAGG TGAAGGTCAA ACAGGAGCCA 
GGAGTTGAGG AAGAATGTAG CTATACTGGA GCAAACGTCA AAACTGAACG AACTAAAGAT 
GGCAGACGGA GCGCTTGTAT GATGAGCAGT CCAGAAGGAA GTTTAACTCC CCCTCTCCCC 
ATCCTTGGCT CCGTGTCCAC CGGCTCAGTC CAGGACATTT TAAGGACACT TGGTGAAAAC 
GTTAAATCTG AACCACAGAG CGATAACCTT TCCTCCTGCA CGAACCCGAA CTCCAGAGCA 
ACTCTAACCA ATGGGACCAG CGGGTCGAAT GGAGGCCAGC GAGGCGGCAC TACTAATGCT 
AACAGTCAGA CCACTGCGGG AAAGGAAGAC GACCCGAATG AAGACTGGTG TGCTGTTTGT 
CAGAACGGAG GAGAACTGCT GTGCTGCGAT CACTGTCCCA AAGTCTTCCA CATAACCTGT 
CATATCCCAA CACTCAAATC CTCTCCCAGT GGGGATTGGA TGTGCACGTT CTGCAGAAAT 
CTCGCAAATC CAGAGATCGA GTACAACTGT GATGACGATC CACCCAGAAA CAAAGAAAAA 
AATGAGATGG CAATGAGTCC AGAAGAACAA AGGCGATGTG AGCGTCTGCT GCTGCATGTT 
TTCTGTCATG AGCTCAGCAC AGAGTTTCAA GAGCCCGTTC CTACCTCGGT TCCTAATTAC 
TATAAAATAA TAAAGCATCC GATGGATTTG ACCCTGGTGA AGCGGAAACT ACAGAGAAAG 
CATCCTCTGC ATTACAAGAG CCCTAAAGAG TTTGTGTCGG ACGTGCGCCT TGTCTTTAGC 
AATTGTGCCA AATATAATGA GGCCGATTCG GAAGTGGCAG AAGCTGGAAA AGCCGTGAGT 
CTGTACTTTG AGGAGCGATT GCTGGAGATT TTTCCAGAGC AGACGTTCCC GGTGGTGATG 
GAGAAGGAAA CTCAAATCGA GGCCGAAAAG GAGGATTCGG ACGACTCCGA TGATGACATC 
ATACAACCCA AACGCAAACG CTTAAAAGTG GACACAGAGA TGCTGCTGCA CATCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trim33 ( XM_005166728.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166785.1 Danio rerio tripartite motif containing 33 (trim33), transcript variant X1, mRNA.

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CloneID	ODa13905
Clone ID Related Accession (Same CDS sequence)	XM_005166728.4 , XM_005166728.3
Accession Version	XM_005166728.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase TRIM33 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005166728.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACA ACAAAGGCCA AGATTGTACG GAGAGCTCCG CAAATATGGA GGTAGAGGCG 
TCGGGGACGG AAGAAAACGG AGACGGTGGC GCAAAAGAGC CTGAGGCGGT TGTTTCAATC 
GACACGGAGC CGAAAGAAGC AGCAGACGAG GCCAAATCTC AAGAGACCGT TCCCCAGGCG 
CCGACGACAT CCAGCGATAG CAGCAGCAGT GGCGGCGGCG GCGAAGCTAC AGACGGGACC 
CCGAATGCTG AAAGCGCGGA TCCTCCTCCT CCACCACCAC CACCTCCGCC GCCGCCACCG 
TCCACCCCAG CGGACAGCAC CGCAGCTGCC GCATCACCCG CTGTGGTTAC CGAGATGTCG 
CCTCCTCCAC CAGCATCCAC ATCTTCATCA TCTTCCACCC CTGCCGCCCC GATAAACCTG 
CTGGATACGT GCGCTGTGTG TAAACAGAGT CTCCAGAACC GCGACTGTGA ACCCAAACTC 
CTGCCCTGCT TGCACTCCTT TTGCCTCAAG TGCATCCCAC AGCCCGATCG TAAGATCACC 
ATGCCGGTGC AAGGCCCCCA CGGACAGGAC ACTCGAATCG TGAACGTCAT GCGTTGTACG 
GTGTGCCATC AGGACTACAA ACAGATTGAC ATGGTGGACA ATTACTTCGT CAAGGACACT 
TCAGAGGCCA CAAACACGTC AGTGGAGAAC TCGACACAGG TGTGCACCAG CTGTGAAGAT 
AATGCCAGTG CTATTGGATT CTGCGTTGAA TGTGGAGAGT GGCTCTGCAA AACTTGCATC 
GAAGCGCACC AAAGGGTCAA ATTTACCAAG GACCATAAAA TACGCAAAAA AGAGGAAGTG 
TCTCCTGAGT CTGTTGGGAC ATCAGGCCAG AGACCAGTTT TCTGTCCGGT ACACAAACAA 
GAGGCCCTCA AGCTCTTCTG TGAGACCTGC GACACTCTGA CCTGCAGAGA CTGCCAGCTA 
CTTGAACACA AAGAACACAG GTACCAGTTT CTAGATGAAG CCTGTCAAAA CCAGAAAGGC 
ATCATTGCGA CATTCATGAC TAAGTTACAG GAGAAGAGAG GCTTAGTGGA GTACTCTGCA 
TCTGAAGTGC AAAAAAGACT CAAAGAAGTT GCTGAGACGC ATAAGAAGGT GGAACATGAG 
ATAAAGATAG CAGTCTTTAC TCTTATCAAT GAAATCAACA AGAAGGGGAA ATCTCTGCTG 
CAGCAGTTGG AGAGTGTGAC CAAAGACAGG AGCATGAAGC TGCTGAGCCA ACAGAGGGAC 
ATCAGTGTTC TGGCCCAGCA GATCATCCAT GTGCTCAACT TCACCAACTG GGCCATCACT 
AACGGCAGCA GCACTGCCCT GCTCTACAGC AAGAGACTGA TCACGTACCA GCTGCGTCTG 
ATTATGAAGG CCCGTGTGGA TGCAGTACCT CCAGCTAATG GAGCAGTTCG CTTCTTCTGT 
GACCCAACCT TTTGGGCCAA GAATGTGGTT AACCTTGGCA ATCTAGTCAT TGAAAAAGTT 
GCTCCTACTG CTCCTCCGTC CAACATGATG GTCAATCAGC AGATCTCTCC CGGTCACAAC 
CACCCAGGTA AGCACTCAGG ACAGATCAAC CTGGCCCAGC TGAGGCTGCA GCACATGCAG 
CAGGCAGCGA TTGCTCAGAA ACATCAGCAG CAACACCAAC ACCACCAACA ACAACAACAC 
CAACACCAAC AACAGCAGCA GCAACAACAA CAACAACAAC AACAACAGCA GCAGCAACAA 
CAACAACAGC AACACCAGCA ACAAATTCAG CAGCAGATGC GCATCGCCTC TCAGATGTCC 
CAGCATCCCA GACAAGGAGC TCCACAAATG ATTCAGCAAC CTCCTCCGAG ACTCATTAGT 
ATGCAGGCCC TCCACAGGGG CGGAGTTAAT GGTTCCTCCC ACATGTTTCC GCCTCATCAC 
CTGCGCATGG TGTCAGCGCA GAACCGCATG CCTTCTGCTC AACCTCGACT CAATGGCCAG 
CCATATCCCA TGATGCAACC TCAACTTCAG AGACAGCACT CTAATCCTGG GCATGCGGGT 
CCATTCCCTG TGGCGTCGCT ACACAACATT AGTGCTGCCA ACCCCACCAG TCCCACCAGT 
GCCAGTATGG CCAATGCCCA TATGCACCGC GGCCCCTCCA GTCCTGTTAT CACCCCTATA 
GAGCTCATAC CGTCAGTCAC CAACCCTGAG AACCTGCCCT GCCTACCCGA CATCCCTCCT 
ATTCAGCTTG AAGATGCCGG CTCTAGCACA CTGGATAATA TCCTAAGTCG GTATATTTCT 
GCTAATGCAT ATCCTACTGT CGGACCCACA AACCCATCGC CAGGACCTTC CACACATTCT 
CCGGGATCGT CAGGTTTATC TAATTCTCAC ACTCCAGCTC GTCCATCCAG CACGTCAAGT 
ACAGGCAGTC GGGGCAGTTC TGGAACCACA GTGGACCAGG TGAAGGTCAA ACAGGAGCCA 
GGAGTTGAGG AAGAATGTAG CTATACTGGA GCAAACGTCA AAACTGAACG AACTAAAGAT 
GGCAGACGGA GCGCTTGTAT GATGAGCAGT CCAGAAGGAA GTTTAACTCC CCCTCTCCCC 
ATCCTTGGCT CCGTGTCCAC CGGCTCAGTC CAGGACATTT TAAGGACACT TGGTGAAAAC 
GTTAAATCTG AACCACAGAG CGATAACCTT TCCTCCTGCA CGAACCCGAA CTCCAGAGCA 
ACTCTAACCA ATGGGACCAG CGGGTCGAAT GGAGGCCAGC GAGGCGGCAC TACTAATGCT 
AACAGTCAGA CCACTGCGGG AAAGGAAGAC GACCCGAATG AAGACTGGTG TGCTGTTTGT 
CAGAACGGAG GAGAACTGCT GTGCTGCGAT CACTGTCCCA AAGTCTTCCA CATAACCTGT 
CATATCCCAA CACTCAAATC CTCTCCCAGT GGGGATTGGA TGTGCACGTT CTGCAGAAAT 
CTCGCAAATC CAGAGATCGA GTACAACTGT GATGACGATC CACCCAGAAA CAAAGAAAAA 
AATGAGATGG CAATGAGTCC AGAAGAACAA AGGCGATGTG AGCGTCTGCT GCTGCATGTT 
TTCTGTCATG AGCTCAGCAC AGAGTTTCAA GAGCCCGTTC CTACCTCGGT TCCTAATTAC 
TATAAAATAA TAAAGCATCC GATGGATTTG ACCCTGGTGA AGCGGAAACT ACAGAGAAAG 
CATCCTCTGC ATTACAAGAG CCCTAAAGAG TTTGTGTCGG ACGTGCGCCT TGTCTTTAGC 
AATTGTGCCA AATATAATGA GGCCGATTCG GAAGTGGCAG AAGCTGGAAA AGCCGTGAGT 
CTGTACTTTG AGGAGCGATT GCTGGAGATT TTTCCAGAGC AGACGTTCCC GGTGGTGATG 
GAGAAGGAAA CTCAAATCGA GGCCGAAAAG GAGGATTCGG ACGACTCCGA TGATGACATC 
ATACAACCCA AACGCAAACG CTTAAAAGTG GACACAGAGA TGCTGCTGCA CATCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166785.1
CDS	174..3653
Translation	MADNKGQDCTESSANMEVEASGTEENGDGGAKEPEAVVSIDTEPKEAADEAKSQETVPQAPTTSSDSSSSGGGGEATDGTPNAESADPPPPPPPPPPPPPSTPADSTAAAASPAVVTEMSPPPPASTSSSSSTPAAPINLLDTCAVCKQSLQNRDCEPKLLPCLHSFCLKCIPQPDRKITMPVQGPHGQDTRIVNVMRCTVCHQDYKQIDMVDNYFVKDTSEATNTSVENSTQVCTSCEDNASAIGFCVECGEWLCKTCIEAHQRVKFTKDHKIRKKEEVSPESVGTSGQRPVFCPVHKQEALKLFCETCDTLTCRDCQLLEHKEHRYQFLDEACQNQKGIIATFMTKLQEKRGLVEYSASEVQKRLKEVAETHKKVEHEIKIAVFTLINEINKKGKSLLQQLESVTKDRSMKLLSQQRDISVLAQQIIHVLNFTNWAITNGSSTALLYSKRLITYQLRLIMKARVDAVPPANGAVRFFCDPTFWAKNVVNLGNLVIEKVAPTAPPSNMMVNQQISPGHNHPGKHSGQINLAQLRLQHMQQAAIAQKHQQQHQHHQQQQHQHQQQQQQQQQQQQQQQQQQQQQQHQQQIQQQMRIASQMSQHPRQGAPQMIQQPPPRLISMQALHRGGVNGSSHMFPPHHLRMVSAQNRMPSAQPRLNGQPYPMMQPQLQRQHSNPGHAGPFPVASLHNISAANPTSPTSASMANAHMHRGPSSPVITPIELIPSVTNPENLPCLPDIPPIQLEDAGSSTLDNILSRYISANAYPTVGPTNPSPGPSTHSPGSSGLSNSHTPARPSSTSSTGSRGSSGTTVDQVKVKQEPGVEEECSYTGANVKTERTKDGRRSACMMSSPEGSLTPPLPILGSVSTGSVQDILRTLGENVKSEPQSDNLSSCTNPNSRATLTNGTSGSNGGQRGGTTNANSQTTAGKEDDPNEDWCAVCQNGGELLCCDHCPKVFHITCHIPTLKSSPSGDWMCTFCRNLANPEIEYNCDDDPPRNKEKNEMAMSPEEQRRCERLLLHVFCHELSTEFQEPVPTSVPNYYKIIKHPMDLTLVKRKLQRKHPLHYKSPKEFVSDVRLVFSNCAKYNEADSEVAEAGKAVSLYFEERLLEIFPEQTFPVVMEKETQIEAEKEDSDDSDDDIIQPKRKRLKVDTEMLLHIK

Target ORF information:

RefSeq Version	XM_005166728.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tripartite motif containing 33 (trim33), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166728.3

ORF Insert Sequence:

ATGGCGGACA ACAAAGGCCA AGATTGTACG GAGAGCTCCG CAAATATGGA GGTAGAGGCG 
TCGGGGACGG AAGAAAACGG AGACGGTGGC GCAAAAGAGC CTGAGGCGGT TGTTTCAATC 
GACACGGAGC CGAAAGAAGC AGCAGACGAG GCCAAATCTC AAGAGACCGT TCCCCAGGCG 
CCGACGACAT CCAGCGATAG CAGCAGCAGT GGCGGCGGCG GCGAAGCTAC AGACGGGACC 
CCGAATGCTG AAAGCGCGGA TCCTCCTCCT CCACCACCAC CACCTCCGCC GCCGCCACCG 
TCCACCCCAG CGGACAGCAC CGCAGCTGCC GCATCACCCG CTGTGGTTAC CGAGATGTCG 
CCTCCTCCAC CAGCATCCAC ATCTTCATCA TCTTCCACCC CTGCCGCCCC GATAAACCTG 
CTGGATACGT GCGCTGTGTG TAAACAGAGT CTCCAGAACC GCGACTGTGA ACCCAAACTC 
CTGCCCTGCT TGCACTCCTT TTGCCTCAAG TGCATCCCAC AGCCCGATCG TAAGATCACC 
ATGCCGGTGC AAGGCCCCCA CGGACAGGAC ACTCGAATCG TGAACGTCAT GCGTTGTACG 
GTGTGCCATC AGGACTACAA ACAGATTGAC ATGGTGGACA ATTACTTCGT CAAGGACACT 
TCAGAGGCCA CAAACACGTC AGTGGAGAAC TCGACACAGG TGTGCACCAG CTGTGAAGAT 
AATGCCAGTG CTATTGGATT CTGCGTTGAA TGTGGAGAGT GGCTCTGCAA AACTTGCATC 
GAAGCGCACC AAAGGGTCAA ATTTACCAAG GACCATAAAA TACGCAAAAA AGAGGAAGTG 
TCTCCTGAGT CTGTTGGGAC ATCAGGCCAG AGACCAGTTT TCTGTCCGGT ACACAAACAA 
GAGGCCCTCA AGCTCTTCTG TGAGACCTGC GACACTCTGA CCTGCAGAGA CTGCCAGCTA 
CTTGAACACA AAGAACACAG GTACCAGTTT CTAGATGAAG CCTGTCAAAA CCAGAAAGGC 
ATCATTGCGA CATTCATGAC TAAGTTACAG GAGAAGAGAG GCTTAGTGGA GTACTCTGCA 
TCTGAAGTGC AAAAAAGACT CAAAGAAGTT GCTGAGACGC ATAAGAAGGT GGAACATGAG 
ATAAAGATAG CAGTCTTTAC TCTTATCAAT GAAATCAACA AGAAGGGGAA ATCTCTGCTG 
CAGCAGTTGG AGAGTGTGAC CAAAGACAGG AGCATGAAGC TGCTGAGCCA ACAGAGGGAC 
ATCAGTGTTC TGGCCCAGCA GATCATCCAT GTGCTCAACT TCACCAACTG GGCCATCACT 
AACGGCAGCA GCACTGCCCT GCTCTACAGC AAGAGACTGA TCACGTACCA GCTGCGTCTG 
ATTATGAAGG CCCGTGTGGA TGCAGTACCT CCAGCTAATG GAGCAGTTCG CTTCTTCTGT 
GACCCAACCT TTTGGGCCAA GAATGTGGTT AACCTTGGCA ATCTAGTCAT TGAAAAAGTT 
GCTCCTACTG CTCCTCCGTC CAACATGATG GTCAATCAGC AGATCTCTCC CGGTCACAAC 
CACCCAGGTA AGCACTCAGG ACAGATCAAC CTGGCCCAGC TGAGGCTGCA GCACATGCAG 
CAGGCAGCGA TTGCTCAGAA ACATCAGCAG CAACACCAAC ACCACCAACA ACAACAACAC 
CAACACCAAC AACAGCAGCA GCAACAACAA CAACAACAAC AACAACAGCA GCAGCAACAA 
CAACAACAGC AACACCAGCA ACAAATTCAG CAGCAGATGC GCATCGCCTC TCAGATGTCC 
CAGCATCCCA GACAAGGAGC TCCACAAATG ATTCAGCAAC CTCCTCCGAG ACTCATTAGT 
ATGCAGGCCC TCCACAGGGG CGGAGTTAAT GGTTCCTCCC ACATGTTTCC GCCTCATCAC 
CTGCGCATGG TGTCAGCGCA GAACCGCATG CCTTCTGCTC AACCTCGACT CAATGGCCAG 
CCATATCCCA TGATGCAACC TCAACTTCAG AGACAGCACT CTAATCCTGG GCATGCGGGT 
CCATTCCCTG TGGCGTCGCT ACACAACATT AGTGCTGCCA ACCCCACCAG TCCCACCAGT 
GCCAGTATGG CCAATGCCCA TATGCACCGC GGCCCCTCCA GTCCTGTTAT CACCCCTATA 
GAGCTCATAC CGTCAGTCAC CAACCCTGAG AACCTGCCCT GCCTACCCGA CATCCCTCCT 
ATTCAGCTTG AAGATGCCGG CTCTAGCACA CTGGATAATA TCCTAAGTCG GTATATTTCT 
GCTAATGCAT ATCCTACTGT CGGACCCACA AACCCATCGC CAGGACCTTC CACACATTCT 
CCGGGATCGT CAGGTTTATC TAATTCTCAC ACTCCAGCTC GTCCATCCAG CACGTCAAGT 
ACAGGCAGTC GGGGCAGTTC TGGAACCACA GTGGACCAGG TGAAGGTCAA ACAGGAGCCA 
GGAGTTGAGG AAGAATGTAG CTATACTGGA GCAAACGTCA AAACTGAACG AACTAAAGAT 
GGCAGACGGA GCGCTTGTAT GATGAGCAGT CCAGAAGGAA GTTTAACTCC CCCTCTCCCC 
ATCCTTGGCT CCGTGTCCAC CGGCTCAGTC CAGGACATTT TAAGGACACT TGGTGAAAAC 
GTTAAATCTG AACCACAGAG CGATAACCTT TCCTCCTGCA CGAACCCGAA CTCCAGAGCA 
ACTCTAACCA ATGGGACCAG CGGGTCGAAT GGAGGCCAGC GAGGCGGCAC TACTAATGCT 
AACAGTCAGA CCACTGCGGG AAAGGAAGAC GACCCGAATG AAGACTGGTG TGCTGTTTGT 
CAGAACGGAG GAGAACTGCT GTGCTGCGAT CACTGTCCCA AAGTCTTCCA CATAACCTGT 
CATATCCCAA CACTCAAATC CTCTCCCAGT GGGGATTGGA TGTGCACGTT CTGCAGAAAT 
CTCGCAAATC CAGAGATCGA GTACAACTGT GATGACGATC CACCCAGAAA CAAAGAAAAA 
AATGAGATGG CAATGAGTCC AGAAGAACAA AGGCGATGTG AGCGTCTGCT GCTGCATGTT 
TTCTGTCATG AGCTCAGCAC AGAGTTTCAA GAGCCCGTTC CTACCTCGGT TCCTAATTAC 
TATAAAATAA TAAAGCATCC GATGGATTTG ACCCTGGTGA AGCGGAAACT ACAGAGAAAG 
CATCCTCTGC ATTACAAGAG CCCTAAAGAG TTTGTGTCGG ACGTGCGCCT TGTCTTTAGC 
AATTGTGCCA AATATAATGA GGCCGATTCG GAAGTGGCAG AAGCTGGAAA AGCCGTGAGT 
CTGTACTTTG AGGAGCGATT GCTGGAGATT TTTCCAGAGC AGACGTTCCC GGTGGTGATG 
GAGAAGGAAA CTCAAATCGA GGCCGAAAAG GAGGATTCGG ACGACTCCGA TGATGACATC 
ATACAACCCA AACGCAAACG CTTAAAAGTG GACACAGAGA TGCTGCTGCA CATCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Soybean Meal-Induced Intestinal Inflammation in Zebrafish Is T Cell-Dependent and Has a Th17 Cytokine Profile.
Frontiers in immunology10610(2019)
Coronado M,Solis CJ,Hernandez PP,Feijóo CG

Trim33 is essential for macrophage and neutrophil mobilization to developmental or inflammatory cues.
Journal of cell science130(17)2797-2807(2017 Sep)
Demy DL,Tauzin M,Lancino M,Le Cabec V,Redd M,Murayama E,Maridonneau-Parini I,Trede N,Herbomel P

Transforming Growth Factor β Drives Hemogenic Endothelium Programming and the Transition to Hematopoietic Stem Cells.
Developmental cell38(4)358-70(2016 08)
Monteiro R,Pinheiro P,Joseph N,Peterkin T,Koth J,Repapi E,Bonkhofer F,Kirmizitas A,Patient R

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Genome-wide analysis of the zebrafish Klf family identifies two genes important for erythroid maturation.
Developmental biology403(2)115-27(2015 Jul)
Xue Y,Gao S,Liu F

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trim33 cDNA ORF clone, Danio rerio(zebrafish)

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