cfb cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	cfb
Entrez Gene ID	30604
Full Name	complement factor B
Synonyms	Bf-1,Brre-Bf,bf,zgc:114032
General protein information	Preferred Names complement factor B Names complement factor B complement component factor B
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_131338.2	NP_571413.2	complement factor B precursor
XM_005161333.2	XP_005161390.1	complement factor B isoform X1
XM_021468751.1	XP_021324426.1	complement factor B isoform X1

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	CFB	XP_001113553.2
Pan troglodytes (chimpanzee)	CFB	NP_001009169.1
Danio rerio (zebrafish)	zgc:158446	NP_001077327.1
Mus musculus (house mouse)	Cfb	NP_032224.2
Danio rerio (zebrafish)	bfb	NP_571316.1
Bos taurus (cattle)	CFB	NP_001035616.1
Rattus norvegicus (Norway rat)	Cfb	NP_997631.2
Danio rerio (zebrafish)	cfb	NP_571413.1
Xenopus tropicalis (tropical clawed frog)	LOC100491419	XP_002943021.2
Homo sapiens (human)	CFB	NP_001701.2
Canis lupus familiaris (dog)	CFB	XP_003431724.1

Gene Ontology
Bibliography

Related articles in PubMed

Transcriptomic response and perturbation of toxicity pathways in zebrafish larvae after exposure to graphene quantum dots (GQDs).
Deng S, Jia PP, Zhang JH, Junaid M, Niu A, Ma YB, Fu A, Pei DS
Journal of hazardous materials357146-158(2018 09)

Differential transcriptome analysis of zebrafish (Danio rerio) larvae challenged by Vibrio parahaemolyticus.
Zhang Q, Ji C, Ren J, Zhang Q, Dong X, Zu Y, Jia L, Li W
Journal of fish diseases41(7)1049-1062(2018 Jul)

Innate immune system activation in zebrafish and cellular models of Diamond Blackfan Anemia.
Danilova N, Wilkes M, Bibikova E, Youn MY, Sakamoto KM, Lin S
Scientific reports8(1)5165(2018 03)

Re-evaluating the functional landscape of the cardiovascular system during development.
Takada N, Omae M, Sagawa F, Chi NC, Endo S, Kozawa S, Sato TN
Biology open6(11)1756-1770(2017 Nov)

Anti-Infective Effect of Adhesive Probiotic Lactobacillus in Fish is Correlated With Their Spatial Distribution in the Intestinal Tissue.
He S, Ran C, Qin C, Li S, Zhang H, de Vos WM, Ringø E, Zhou Z
Scientific reports7(1)13195(2017 10)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following cfb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cfb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa39283	NM_131338.2 Latest version!	Danio rerio complement factor B (cfb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa25953	XM_021468751.1 Latest version!	Danio rerio complement factor B (cfb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa25953	XM_005161333.2 Latest version!	Danio rerio complement factor B (cfb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cfb ( NM_131338.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571413.2 Danio rerio complement factor B (cfb), mRNA.

Price & Availability↑

CloneID	ODa39283
Clone ID Related Accession (Same CDS sequence)	NM_131338.2
Accession Version	NM_131338.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550937600000
Organism	Danio rerio(zebrafish)
Product	complement factor B precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX511175.6, EH508237.1, DT057402.1, EH587453.1, DT060807.1, EH531956.1 and EH604900.1. On or before Jun 23, 2015 this sequence version replaced XM_009295464.1, NM_131338.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA3505370, SAMEA3505372 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCAGCA TGGAGTGTGG GCTGCGGTTG AAGTGGTTAA TACTTGCACT GATATGTCCT 
CTCATTGCAG GTGCTCCATC CAGAGAGGGT TCATGTCCCG AAGAAAATCT GGACATTGCA 
GGAGGGAGTT TTACTCTTTC TAATGGCTAT TCGGATGGAA GTTATCTGCA ATACATTTGT 
CCAGATAACC ATTACCCATC TATTTCATCA CGTCGTTGTC AATTTGGAGT CTGGACCCCA 
AAGGCCAGCT CTAGAAAAAA AGCTGAGTGC AAAAAAATCA CATGTCCTAA TCCTCGTGTT 
TTGGAGAATG GAGAGGTGGC TCCATATCAA GAGAGGTACT ATATAAATGA CGTAACTACT 
TACTCATGTA GTTCTGATTA TAAATTTCGA GGCTCGAAAG TCCGGGTTTG CCAACCTAAT 
GGGAAGTGGA ATGGAAGCAC GCCAATTTGT GGACGTGACT ATCACTGTCC TGATCCTGGC 
GTTCCCCCTG GTAGCAGTCG AACAGGCAGC ATATTTAACA TTGATGATGA AGTCACGTAC 
CACTGTGACA GTCCATTGAC CCTGATCGGT TCCAAAGTGC GAAAGTGTCT GGATGATGGC 
CAGTGGTCAG GAACAGAGCC ACAGTGCTAC GCTGATTTCA CATATGACAC TGCAATGGAA 
GCAGCTGAAG CTTTTGGCAA TTCTCTAACG ACAACTCTAA CAGTTCAACA AGGGTTTGAA 
GATGATCAGC ATGGGAAGAA AATATCTTTG GATAGGGGTG GAAAACTTGA TATCTACATT 
GCTGTGGATG CATCTGACAG CATTGACCCG AAGGATTTTG GCAAAGCAAA AAAAATTATT 
AAAACACTCA TAGAAAAGAT TAGTTATTAT GAGGTCTCTC CGAACTATGA GATCCTGATG 
TTTGCGACGG ATGTTGATCA GATAGTTAAA ATGAGGGATT TCAAAACTAA TGAAAAGGCA 
AGAAACATCT CGAAGATTTT TGAAGATCTG GACAACTTTA ACTACGACAA AAAAGGCGAT 
CGAACAGGCA CTAATATCGC TAAACTTTAC TTGAAGATTT TAGACAGCAT GTCACTTGAG 
CAGGTCCAGA ATAAAGAAGA CTTTCTTCAG ACCCAACACG TCATCATCGT GTTTACTGAT 
GGCCAAGCTA ACATGGGAGG GAATCCCAAA CCTAAAGTGG ACCTAATAAA GAATCTTGTC 
ATTAAAAATA ATGCAAGTCG AGAGAATAAA CTTGATCTTT ATGTATTTGG AGTTGGAAAG 
GATGTAAAGA AAGAAGACAT GAATGGATTA GTGTCAGAGA AGAAAGATGA AAGACATTTT 
TTTAAGCTTC CAGACTTGGA CGAGGTGCAG AATACGTTTG ACCTCATGCT TGATGATAGC 
ACCGTTGTAG GATTGTGTGG GATGCAGCAA AACTATGATG GATCAAATAA ACGCAGCGCA 
TACCCCTGGT TGGCACAGCT TAGTATTGCA CAATCGCAGA TCTCAGATTG CATGGGCTCG 
TTAGTTACCT CCCGGTATAT CTTGACAGCA GCCCACTGTT TTAAAGAAGG AGACACACCG 
GATAAGATAA CGGTGTATTT GGAAAAAAAC ACGGATGTAA AAGTGGAAAA GGTTTTCATT 
CATCCTAATT ATAGCCTCAC AGCAAAACAA AGCATTGGAA TAAAGGAATT TTATGACTTT 
GATGTCGCAC TTCTGCAGCT CAAAACTCCT GTTAAGATGA GCGTCAATCT ACGGCCAATC 
TGCCTCCCCT GCACCAAAGA AACCAATAGA GCACTGAAAC TTTCAGACAG TCAAGGGACT 
TGTGAAAAAC ATGAGCAAAT ATTATTGAGC AATGAGCTTG TGGATGCTGC TTTCACATCT 
AAAATGGACA TGGAAAAACG CAGTCCACGG AAAATAAGGA GGATCACAGT CAAGCTTGGA 
AAATATCTGG ATGCTTGTGT TGAAGATGCA AAAAAAGCAA AAGGAATCGA AGTGGCAGAC 
GCGACGGAGG CAGTTACAAA AAATTTCTTG TGCAGTGGTG GTAATCAACC ACAAAGAGAT 
GATGTTTCCT GCAAAGGTGA ATCAGGTGGA GCCACACATG TGGATAAATA TGGCCGACTG 
ATTCAGATTG GTGTTGTTAG CTGGGGAGTG AAGAACCTCT GCTCAAAAGC GAAACTTGAT 
GCAGTTTCGG TTTCAGACTC CAGAGATTAC CATATCAATC TTTTAAGACC AGATATACAG 
TCATTCCTTA AAAAGCACCT GGAAAATGAC AAATTAGGAC ACACTCTTAC TTTCTTGCCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571413.2
CDS	50..2332
Translation	MTSMECGLRLKWLILALICPLIAGAPSREGSCPEENLDIAGGSFTLSNGYSDGSYLQYICPDNHYPSISSRRCQFGVWTPKASSRKKAECKKITCPNPRVLENGEVAPYQERYYINDVTTYSCSSDYKFRGSKVRVCQPNGKWNGSTPICGRDYHCPDPGVPPGSSRTGSIFNIDDEVTYHCDSPLTLIGSKVRKCLDDGQWSGTEPQCYADFTYDTAMEAAEAFGNSLTTTLTVQQGFEDDQHGKKISLDRGGKLDIYIAVDASDSIDPKDFGKAKKIIKTLIEKISYYEVSPNYEILMFATDVDQIVKMRDFKTNEKARNISKIFEDLDNFNYDKKGDRTGTNIAKLYLKILDSMSLEQVQNKEDFLQTQHVIIVFTDGQANMGGNPKPKVDLIKNLVIKNNASRENKLDLYVFGVGKDVKKEDMNGLVSEKKDERHFFKLPDLDEVQNTFDLMLDDSTVVGLCGMQQNYDGSNKRSAYPWLAQLSIAQSQISDCMGSLVTSRYILTAAHCFKEGDTPDKITVYLEKNTDVKVEKVFIHPNYSLTAKQSIGIKEFYDFDVALLQLKTPVKMSVNLRPICLPCTKETNRALKLSDSQGTCEKHEQILLSNELVDAAFTSKMDMEKRSPRKIRRITVKLGKYLDACVEDAKKAKGIEVADATEAVTKNFLCSGGNQPQRDDVSCKGESGGATHVDKYGRLIQIGVVSWGVKNLCSKAKLDAVSVSDSRDYHINLLRPDIQSFLKKHLENDKLGHTLTFLP

Target ORF information:

RefSeq Version	NM_131338.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio complement factor B (cfb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131338.2

ORF Insert Sequence:

ATGACCAGCA TGGAGTGTGG GCTGCGGTTG AAGTGGTTAA TACTTGCACT GATATGTCCT 
CTCATTGCAG GTGCTCCATC CAGAGAGGGT TCATGTCCCG AAGAAAATCT GGACATTGCA 
GGAGGGAGTT TTACTCTTTC TAATGGCTAT TCGGATGGAA GTTATCTGCA ATACATTTGT 
CCAGATAACC ATTACCCATC TATTTCATCA CGTCGTTGTC AATTTGGAGT CTGGACCCCA 
AAGGCCAGCT CTAGAAAAAA AGCTGAGTGC AAAAAAATCA CATGTCCTAA TCCTCGTGTT 
TTGGAGAATG GAGAGGTGGC TCCATATCAA GAGAGGTACT ATATAAATGA CGTAACTACT 
TACTCATGTA GTTCTGATTA TAAATTTCGA GGCTCGAAAG TCCGGGTTTG CCAACCTAAT 
GGGAAGTGGA ATGGAAGCAC GCCAATTTGT GGACGTGACT ATCACTGTCC TGATCCTGGC 
GTTCCCCCTG GTAGCAGTCG AACAGGCAGC ATATTTAACA TTGATGATGA AGTCACGTAC 
CACTGTGACA GTCCATTGAC CCTGATCGGT TCCAAAGTGC GAAAGTGTCT GGATGATGGC 
CAGTGGTCAG GAACAGAGCC ACAGTGCTAC GCTGATTTCA CATATGACAC TGCAATGGAA 
GCAGCTGAAG CTTTTGGCAA TTCTCTAACG ACAACTCTAA CAGTTCAACA AGGGTTTGAA 
GATGATCAGC ATGGGAAGAA AATATCTTTG GATAGGGGTG GAAAACTTGA TATCTACATT 
GCTGTGGATG CATCTGACAG CATTGACCCG AAGGATTTTG GCAAAGCAAA AAAAATTATT 
AAAACACTCA TAGAAAAGAT TAGTTATTAT GAGGTCTCTC CGAACTATGA GATCCTGATG 
TTTGCGACGG ATGTTGATCA GATAGTTAAA ATGAGGGATT TCAAAACTAA TGAAAAGGCA 
AGAAACATCT CGAAGATTTT TGAAGATCTG GACAACTTTA ACTACGACAA AAAAGGCGAT 
CGAACAGGCA CTAATATCGC TAAACTTTAC TTGAAGATTT TAGACAGCAT GTCACTTGAG 
CAGGTCCAGA ATAAAGAAGA CTTTCTTCAG ACCCAACACG TCATCATCGT GTTTACTGAT 
GGCCAAGCTA ACATGGGAGG GAATCCCAAA CCTAAAGTGG ACCTAATAAA GAATCTTGTC 
ATTAAAAATA ATGCAAGTCG AGAGAATAAA CTTGATCTTT ATGTATTTGG AGTTGGAAAG 
GATGTAAAGA AAGAAGACAT GAATGGATTA GTGTCAGAGA AGAAAGATGA AAGACATTTT 
TTTAAGCTTC CAGACTTGGA CGAGGTGCAG AATACGTTTG ACCTCATGCT TGATGATAGC 
ACCGTTGTAG GATTGTGTGG GATGCAGCAA AACTATGATG GATCAAATAA ACGCAGCGCA 
TACCCCTGGT TGGCACAGCT TAGTATTGCA CAATCGCAGA TCTCAGATTG CATGGGCTCG 
TTAGTTACCT CCCGGTATAT CTTGACAGCA GCCCACTGTT TTAAAGAAGG AGACACACCG 
GATAAGATAA CGGTGTATTT GGAAAAAAAC ACGGATGTAA AAGTGGAAAA GGTTTTCATT 
CATCCTAATT ATAGCCTCAC AGCAAAACAA AGCATTGGAA TAAAGGAATT TTATGACTTT 
GATGTCGCAC TTCTGCAGCT CAAAACTCCT GTTAAGATGA GCGTCAATCT ACGGCCAATC 
TGCCTCCCCT GCACCAAAGA AACCAATAGA GCACTGAAAC TTTCAGACAG TCAAGGGACT 
TGTGAAAAAC ATGAGCAAAT ATTATTGAGC AATGAGCTTG TGGATGCTGC TTTCACATCT 
AAAATGGACA TGGAAAAACG CAGTCCACGG AAAATAAGGA GGATCACAGT CAAGCTTGGA 
AAATATCTGG ATGCTTGTGT TGAAGATGCA AAAAAAGCAA AAGGAATCGA AGTGGCAGAC 
GCGACGGAGG CAGTTACAAA AAATTTCTTG TGCAGTGGTG GTAATCAACC ACAAAGAGAT 
GATGTTTCCT GCAAAGGTGA ATCAGGTGGA GCCACACATG TGGATAAATA TGGCCGACTG 
ATTCAGATTG GTGTTGTTAG CTGGGGAGTG AAGAACCTCT GCTCAAAAGC GAAACTTGAT 
GCAGTTTCGG TTTCAGACTC CAGAGATTAC CATATCAATC TTTTAAGACC AGATATACAG 
TCATTCCTTA AAAAGCACCT GGAAAATGAC AAATTAGGAC ACACTCTTAC TTTCTTGCCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cfb ( XM_005161333.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161390.1 Danio rerio complement factor B (cfb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa25953
Clone ID Related Accession (Same CDS sequence)	XM_005161333.2 , XM_021468751.1
Accession Version	XM_005161333.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	complement factor B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005161333.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAGCA TGGAGTGTGG GCTGCGGTTG AAGTGGTTAA TACTTGCACT GATATGTCCT 
CTCATTGCAG GTGCTCCATC CAGAGAGGGT TCATGTCCCG AAGAAAATCT GGACATTGCA 
GGAGGGAGTT TTACTCTTTC TAATGGCTAT TCGGATGGAA GTTATCTGCA ATACATTTGT 
CCAGATAACC ATTACCCATC TATTTCATCA CGTCGTTGTC AATTTGGAGT CTGGACCCCA 
AAGGCCAGCT CTAGAAAAAA AGCTGAGTGC AAAAAAATCA CATGTCCTAA TCCTCGTGTT 
TTGGAGAATG GAGAGGTGGC TCCATATCAA GAGAGGTACT ATATAAATGA CGTAACTACT 
TACTCATGTA GTTCTGATTA TAAATTTCGA GGCTCGAAAG TCCGGGTTTG CCAACCTAAT 
GGGAAGTGGA ATGGAAGCAC GCCAATTTGT GGACGTGACT CAGATCACTG TCCTGATCCT 
GGCGTTCCCC CTGGTAGCAG TCGAACAGGC AGCATATTTA ACATTGATGA TGAAGTCACG 
TACCACTGTG ACAGTCCATT GACCCTGATC GGTTCCAAAG TGCGAAAGTG TCTGGATGAT 
GGCCAGTGGT CAGGAACAGA GCCACAGTGC TACGCTGATT TCACATATGA CACTGCAATG 
GAAGCAGCTG AAGCTTTTGG CAATTCTCTA ACGACAACTC TAACAGTTCA ACAAGGGTTT 
GAAGATGATC AGCATGGGAA GAAAATATCT TTGGATAGGG GTGGAAAACT TGATATCTAC 
ATTGCTGTGG ATGCATCTGA CAGCATTGAC CCGAAGGATT TTGGCAAAGC AAAAAAAATT 
ATTAAAACAC TCATAGAAAA GATTAGTTAT TATGAGGTCT CTCCGAACTA TGAGATCCTG 
ATGTTTGCGA CGGATGTTGA TCAGATAGTT AAAATGAGGG ATTTCAAAAC TAATGAAAAG 
GCAAGAAACA TCTCGAAGAT TTTTGAAGAT CTGGACAACT TTAACTACGA CAAAAAAGGC 
GATCGAACAG GCACTAATAT CGCTAAACTT TACTTGAAGA TTTTAGACAG CATGTCACTT 
GAGCAGGTCC AGAATAAAGA AGACTTTCTT CAGACCCAAC ACGTCATCAT CGTGTTTACT 
GATGGCCAAG CTAACATGGG AGGGAATCCC AAACCTAAAG TGGACCTAAT AAAGAATCTT 
GTCATTAAAA ATAATGCAAG TCGAGAGAAT AAACTTGATC TTTATGTATT TGGAGTTGGA 
AAGGATGTAA AGAAAGAAGA CATGAATGGA TTAGTGTCAG AGAAGAAAGA TGAAAGACAT 
TTTTTTAAGC TTCCAGACTT GGACGAGGTG CAGAATACGT TTGACCTCAT GCTTGATGAT 
AGCACCGTTG TAGGATTGTG TGGGATGCAG CAAAACTATG ATGGATCAAA TAAACGCAGC 
GCATACCCCT GGTTGGCACA GCTTAGTATT GCACAATCGC AGATCTCAGA TTGCATGGGC 
TCGTTAGTTA CCTCCCGGTA TATCTTGACA GCAGCCCACT GTTTTAAAGA AGGAGACACA 
CCGGATAAGA TAACGGTGTA TTTGGAAAAA AACACGGATG TAAAAGTGGA AAAGGTTTTC 
ATTCATCCTA ATTATAGCCT CACAGCAAAA CAAAGCATTG GAATAAAGGA ATTTTATGAC 
TTTGATGTCG CACTTCTGCA GCTCAAAACT CCTGTTAAGA TGAGCGTCAA TCTACGGCCA 
ATCTGCCTCC CCTGCACCAA AGAAACCAAT AGAGCACTGA AACTTTCAGA CAGTCAAGGG 
ACTTGTGAAA AACATGAGCA AATATTATTG AGCAATGAGC TTGTGGATGC TGCTTTCACA 
TCTAAAATGG ACATGGAAAA ACGCAGTCCA CGGAAAATAA GGAGGATCAC AGTCAAGCTT 
GGAAAATATC TGGATGCTTG TGTTGAAGAT GCAAAAAAAG CAAAAGGAAT CGAAGTGGCA 
GACGCGACGG AGGCAGTTAC AAAAAATTTC TTGTGCAGTG GTGGTAATCA ACCACAAAGA 
GATGATGTTT CCTGCAAAGG TGAATCAGGT GGAGCCACAC ATGTGGATAA ATATGGCCGA 
CTGATTCAGA TTGGTGTTGT TAGCTGGGGA GTGAAGAACC TCTGCTCAAA AGCGAAACTT 
GATGCAGTTT CGGTTTCAGA CTCCAGAGAT TACCATATCA ATCTTTTAAG ACCAGATATA 
CAGTCATTCC TTAAAAAGCA CCTGGAAAAT GACAAATTAG GACACACTCT TACTTTCTTG 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161390.1
CDS	45..2330
Translation	MTSMECGLRLKWLILALICPLIAGAPSREGSCPEENLDIAGGSFTLSNGYSDGSYLQYICPDNHYPSISSRRCQFGVWTPKASSRKKAECKKITCPNPRVLENGEVAPYQERYYINDVTTYSCSSDYKFRGSKVRVCQPNGKWNGSTPICGRDSDHCPDPGVPPGSSRTGSIFNIDDEVTYHCDSPLTLIGSKVRKCLDDGQWSGTEPQCYADFTYDTAMEAAEAFGNSLTTTLTVQQGFEDDQHGKKISLDRGGKLDIYIAVDASDSIDPKDFGKAKKIIKTLIEKISYYEVSPNYEILMFATDVDQIVKMRDFKTNEKARNISKIFEDLDNFNYDKKGDRTGTNIAKLYLKILDSMSLEQVQNKEDFLQTQHVIIVFTDGQANMGGNPKPKVDLIKNLVIKNNASRENKLDLYVFGVGKDVKKEDMNGLVSEKKDERHFFKLPDLDEVQNTFDLMLDDSTVVGLCGMQQNYDGSNKRSAYPWLAQLSIAQSQISDCMGSLVTSRYILTAAHCFKEGDTPDKITVYLEKNTDVKVEKVFIHPNYSLTAKQSIGIKEFYDFDVALLQLKTPVKMSVNLRPICLPCTKETNRALKLSDSQGTCEKHEQILLSNELVDAAFTSKMDMEKRSPRKIRRITVKLGKYLDACVEDAKKAKGIEVADATEAVTKNFLCSGGNQPQRDDVSCKGESGGATHVDKYGRLIQIGVVSWGVKNLCSKAKLDAVSVSDSRDYHINLLRPDIQSFLKKHLENDKLGHTLTFLP

Target ORF information:

RefSeq Version	XM_005161333.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio complement factor B (cfb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161333.2

ORF Insert Sequence:

ATGACCAGCA TGGAGTGTGG GCTGCGGTTG AAGTGGTTAA TACTTGCACT GATATGTCCT 
CTCATTGCAG GTGCTCCATC CAGAGAGGGT TCATGTCCCG AAGAAAATCT GGACATTGCA 
GGAGGGAGTT TTACTCTTTC TAATGGCTAT TCGGATGGAA GTTATCTGCA ATACATTTGT 
CCAGATAACC ATTACCCATC TATTTCATCA CGTCGTTGTC AATTTGGAGT CTGGACCCCA 
AAGGCCAGCT CTAGAAAAAA AGCTGAGTGC AAAAAAATCA CATGTCCTAA TCCTCGTGTT 
TTGGAGAATG GAGAGGTGGC TCCATATCAA GAGAGGTACT ATATAAATGA CGTAACTACT 
TACTCATGTA GTTCTGATTA TAAATTTCGA GGCTCGAAAG TCCGGGTTTG CCAACCTAAT 
GGGAAGTGGA ATGGAAGCAC GCCAATTTGT GGACGTGACT CAGATCACTG TCCTGATCCT 
GGCGTTCCCC CTGGTAGCAG TCGAACAGGC AGCATATTTA ACATTGATGA TGAAGTCACG 
TACCACTGTG ACAGTCCATT GACCCTGATC GGTTCCAAAG TGCGAAAGTG TCTGGATGAT 
GGCCAGTGGT CAGGAACAGA GCCACAGTGC TACGCTGATT TCACATATGA CACTGCAATG 
GAAGCAGCTG AAGCTTTTGG CAATTCTCTA ACGACAACTC TAACAGTTCA ACAAGGGTTT 
GAAGATGATC AGCATGGGAA GAAAATATCT TTGGATAGGG GTGGAAAACT TGATATCTAC 
ATTGCTGTGG ATGCATCTGA CAGCATTGAC CCGAAGGATT TTGGCAAAGC AAAAAAAATT 
ATTAAAACAC TCATAGAAAA GATTAGTTAT TATGAGGTCT CTCCGAACTA TGAGATCCTG 
ATGTTTGCGA CGGATGTTGA TCAGATAGTT AAAATGAGGG ATTTCAAAAC TAATGAAAAG 
GCAAGAAACA TCTCGAAGAT TTTTGAAGAT CTGGACAACT TTAACTACGA CAAAAAAGGC 
GATCGAACAG GCACTAATAT CGCTAAACTT TACTTGAAGA TTTTAGACAG CATGTCACTT 
GAGCAGGTCC AGAATAAAGA AGACTTTCTT CAGACCCAAC ACGTCATCAT CGTGTTTACT 
GATGGCCAAG CTAACATGGG AGGGAATCCC AAACCTAAAG TGGACCTAAT AAAGAATCTT 
GTCATTAAAA ATAATGCAAG TCGAGAGAAT AAACTTGATC TTTATGTATT TGGAGTTGGA 
AAGGATGTAA AGAAAGAAGA CATGAATGGA TTAGTGTCAG AGAAGAAAGA TGAAAGACAT 
TTTTTTAAGC TTCCAGACTT GGACGAGGTG CAGAATACGT TTGACCTCAT GCTTGATGAT 
AGCACCGTTG TAGGATTGTG TGGGATGCAG CAAAACTATG ATGGATCAAA TAAACGCAGC 
GCATACCCCT GGTTGGCACA GCTTAGTATT GCACAATCGC AGATCTCAGA TTGCATGGGC 
TCGTTAGTTA CCTCCCGGTA TATCTTGACA GCAGCCCACT GTTTTAAAGA AGGAGACACA 
CCGGATAAGA TAACGGTGTA TTTGGAAAAA AACACGGATG TAAAAGTGGA AAAGGTTTTC 
ATTCATCCTA ATTATAGCCT CACAGCAAAA CAAAGCATTG GAATAAAGGA ATTTTATGAC 
TTTGATGTCG CACTTCTGCA GCTCAAAACT CCTGTTAAGA TGAGCGTCAA TCTACGGCCA 
ATCTGCCTCC CCTGCACCAA AGAAACCAAT AGAGCACTGA AACTTTCAGA CAGTCAAGGG 
ACTTGTGAAA AACATGAGCA AATATTATTG AGCAATGAGC TTGTGGATGC TGCTTTCACA 
TCTAAAATGG ACATGGAAAA ACGCAGTCCA CGGAAAATAA GGAGGATCAC AGTCAAGCTT 
GGAAAATATC TGGATGCTTG TGTTGAAGAT GCAAAAAAAG CAAAAGGAAT CGAAGTGGCA 
GACGCGACGG AGGCAGTTAC AAAAAATTTC TTGTGCAGTG GTGGTAATCA ACCACAAAGA 
GATGATGTTT CCTGCAAAGG TGAATCAGGT GGAGCCACAC ATGTGGATAA ATATGGCCGA 
CTGATTCAGA TTGGTGTTGT TAGCTGGGGA GTGAAGAACC TCTGCTCAAA AGCGAAACTT 
GATGCAGTTT CGGTTTCAGA CTCCAGAGAT TACCATATCA ATCTTTTAAG ACCAGATATA 
CAGTCATTCC TTAAAAAGCA CCTGGAAAAT GACAAATTAG GACACACTCT TACTTTCTTG 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cfb ( XM_021468751.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324426.1 Danio rerio complement factor B (cfb), transcript variant X1, mRNA.

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CloneID	ODa25953
Clone ID Related Accession (Same CDS sequence)	XM_005161333.2 , XM_021468751.1
Accession Version	XM_021468751.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	complement factor B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAGCA TGGAGTGTGG GCTGCGGTTG AAGTGGTTAA TACTTGCACT GATATGTCCT 
CTCATTGCAG GTGCTCCATC CAGAGAGGGT TCATGTCCCG AAGAAAATCT GGACATTGCA 
GGAGGGAGTT TTACTCTTTC TAATGGCTAT TCGGATGGAA GTTATCTGCA ATACATTTGT 
CCAGATAACC ATTACCCATC TATTTCATCA CGTCGTTGTC AATTTGGAGT CTGGACCCCA 
AAGGCCAGCT CTAGAAAAAA AGCTGAGTGC AAAAAAATCA CATGTCCTAA TCCTCGTGTT 
TTGGAGAATG GAGAGGTGGC TCCATATCAA GAGAGGTACT ATATAAATGA CGTAACTACT 
TACTCATGTA GTTCTGATTA TAAATTTCGA GGCTCGAAAG TCCGGGTTTG CCAACCTAAT 
GGGAAGTGGA ATGGAAGCAC GCCAATTTGT GGACGTGACT CAGATCACTG TCCTGATCCT 
GGCGTTCCCC CTGGTAGCAG TCGAACAGGC AGCATATTTA ACATTGATGA TGAAGTCACG 
TACCACTGTG ACAGTCCATT GACCCTGATC GGTTCCAAAG TGCGAAAGTG TCTGGATGAT 
GGCCAGTGGT CAGGAACAGA GCCACAGTGC TACGCTGATT TCACATATGA CACTGCAATG 
GAAGCAGCTG AAGCTTTTGG CAATTCTCTA ACGACAACTC TAACAGTTCA ACAAGGGTTT 
GAAGATGATC AGCATGGGAA GAAAATATCT TTGGATAGGG GTGGAAAACT TGATATCTAC 
ATTGCTGTGG ATGCATCTGA CAGCATTGAC CCGAAGGATT TTGGCAAAGC AAAAAAAATT 
ATTAAAACAC TCATAGAAAA GATTAGTTAT TATGAGGTCT CTCCGAACTA TGAGATCCTG 
ATGTTTGCGA CGGATGTTGA TCAGATAGTT AAAATGAGGG ATTTCAAAAC TAATGAAAAG 
GCAAGAAACA TCTCGAAGAT TTTTGAAGAT CTGGACAACT TTAACTACGA CAAAAAAGGC 
GATCGAACAG GCACTAATAT CGCTAAACTT TACTTGAAGA TTTTAGACAG CATGTCACTT 
GAGCAGGTCC AGAATAAAGA AGACTTTCTT CAGACCCAAC ACGTCATCAT CGTGTTTACT 
GATGGCCAAG CTAACATGGG AGGGAATCCC AAACCTAAAG TGGACCTAAT AAAGAATCTT 
GTCATTAAAA ATAATGCAAG TCGAGAGAAT AAACTTGATC TTTATGTATT TGGAGTTGGA 
AAGGATGTAA AGAAAGAAGA CATGAATGGA TTAGTGTCAG AGAAGAAAGA TGAAAGACAT 
TTTTTTAAGC TTCCAGACTT GGACGAGGTG CAGAATACGT TTGACCTCAT GCTTGATGAT 
AGCACCGTTG TAGGATTGTG TGGGATGCAG CAAAACTATG ATGGATCAAA TAAACGCAGC 
GCATACCCCT GGTTGGCACA GCTTAGTATT GCACAATCGC AGATCTCAGA TTGCATGGGC 
TCGTTAGTTA CCTCCCGGTA TATCTTGACA GCAGCCCACT GTTTTAAAGA AGGAGACACA 
CCGGATAAGA TAACGGTGTA TTTGGAAAAA AACACGGATG TAAAAGTGGA AAAGGTTTTC 
ATTCATCCTA ATTATAGCCT CACAGCAAAA CAAAGCATTG GAATAAAGGA ATTTTATGAC 
TTTGATGTCG CACTTCTGCA GCTCAAAACT CCTGTTAAGA TGAGCGTCAA TCTACGGCCA 
ATCTGCCTCC CCTGCACCAA AGAAACCAAT AGAGCACTGA AACTTTCAGA CAGTCAAGGG 
ACTTGTGAAA AACATGAGCA AATATTATTG AGCAATGAGC TTGTGGATGC TGCTTTCACA 
TCTAAAATGG ACATGGAAAA ACGCAGTCCA CGGAAAATAA GGAGGATCAC AGTCAAGCTT 
GGAAAATATC TGGATGCTTG TGTTGAAGAT GCAAAAAAAG CAAAAGGAAT CGAAGTGGCA 
GACGCGACGG AGGCAGTTAC AAAAAATTTC TTGTGCAGTG GTGGTAATCA ACCACAAAGA 
GATGATGTTT CCTGCAAAGG TGAATCAGGT GGAGCCACAC ATGTGGATAA ATATGGCCGA 
CTGATTCAGA TTGGTGTTGT TAGCTGGGGA GTGAAGAACC TCTGCTCAAA AGCGAAACTT 
GATGCAGTTT CGGTTTCAGA CTCCAGAGAT TACCATATCA ATCTTTTAAG ACCAGATATA 
CAGTCATTCC TTAAAAAGCA CCTGGAAAAT GACAAATTAG GACACACTCT TACTTTCTTG 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324426.1
CDS	61..2346
Translation	MTSMECGLRLKWLILALICPLIAGAPSREGSCPEENLDIAGGSFTLSNGYSDGSYLQYICPDNHYPSISSRRCQFGVWTPKASSRKKAECKKITCPNPRVLENGEVAPYQERYYINDVTTYSCSSDYKFRGSKVRVCQPNGKWNGSTPICGRDSDHCPDPGVPPGSSRTGSIFNIDDEVTYHCDSPLTLIGSKVRKCLDDGQWSGTEPQCYADFTYDTAMEAAEAFGNSLTTTLTVQQGFEDDQHGKKISLDRGGKLDIYIAVDASDSIDPKDFGKAKKIIKTLIEKISYYEVSPNYEILMFATDVDQIVKMRDFKTNEKARNISKIFEDLDNFNYDKKGDRTGTNIAKLYLKILDSMSLEQVQNKEDFLQTQHVIIVFTDGQANMGGNPKPKVDLIKNLVIKNNASRENKLDLYVFGVGKDVKKEDMNGLVSEKKDERHFFKLPDLDEVQNTFDLMLDDSTVVGLCGMQQNYDGSNKRSAYPWLAQLSIAQSQISDCMGSLVTSRYILTAAHCFKEGDTPDKITVYLEKNTDVKVEKVFIHPNYSLTAKQSIGIKEFYDFDVALLQLKTPVKMSVNLRPICLPCTKETNRALKLSDSQGTCEKHEQILLSNELVDAAFTSKMDMEKRSPRKIRRITVKLGKYLDACVEDAKKAKGIEVADATEAVTKNFLCSGGNQPQRDDVSCKGESGGATHVDKYGRLIQIGVVSWGVKNLCSKAKLDAVSVSDSRDYHINLLRPDIQSFLKKHLENDKLGHTLTFLP

Target ORF information:

RefSeq Version	XM_021468751.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio complement factor B (cfb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468751.1

ORF Insert Sequence:

ATGACCAGCA TGGAGTGTGG GCTGCGGTTG AAGTGGTTAA TACTTGCACT GATATGTCCT 
CTCATTGCAG GTGCTCCATC CAGAGAGGGT TCATGTCCCG AAGAAAATCT GGACATTGCA 
GGAGGGAGTT TTACTCTTTC TAATGGCTAT TCGGATGGAA GTTATCTGCA ATACATTTGT 
CCAGATAACC ATTACCCATC TATTTCATCA CGTCGTTGTC AATTTGGAGT CTGGACCCCA 
AAGGCCAGCT CTAGAAAAAA AGCTGAGTGC AAAAAAATCA CATGTCCTAA TCCTCGTGTT 
TTGGAGAATG GAGAGGTGGC TCCATATCAA GAGAGGTACT ATATAAATGA CGTAACTACT 
TACTCATGTA GTTCTGATTA TAAATTTCGA GGCTCGAAAG TCCGGGTTTG CCAACCTAAT 
GGGAAGTGGA ATGGAAGCAC GCCAATTTGT GGACGTGACT CAGATCACTG TCCTGATCCT 
GGCGTTCCCC CTGGTAGCAG TCGAACAGGC AGCATATTTA ACATTGATGA TGAAGTCACG 
TACCACTGTG ACAGTCCATT GACCCTGATC GGTTCCAAAG TGCGAAAGTG TCTGGATGAT 
GGCCAGTGGT CAGGAACAGA GCCACAGTGC TACGCTGATT TCACATATGA CACTGCAATG 
GAAGCAGCTG AAGCTTTTGG CAATTCTCTA ACGACAACTC TAACAGTTCA ACAAGGGTTT 
GAAGATGATC AGCATGGGAA GAAAATATCT TTGGATAGGG GTGGAAAACT TGATATCTAC 
ATTGCTGTGG ATGCATCTGA CAGCATTGAC CCGAAGGATT TTGGCAAAGC AAAAAAAATT 
ATTAAAACAC TCATAGAAAA GATTAGTTAT TATGAGGTCT CTCCGAACTA TGAGATCCTG 
ATGTTTGCGA CGGATGTTGA TCAGATAGTT AAAATGAGGG ATTTCAAAAC TAATGAAAAG 
GCAAGAAACA TCTCGAAGAT TTTTGAAGAT CTGGACAACT TTAACTACGA CAAAAAAGGC 
GATCGAACAG GCACTAATAT CGCTAAACTT TACTTGAAGA TTTTAGACAG CATGTCACTT 
GAGCAGGTCC AGAATAAAGA AGACTTTCTT CAGACCCAAC ACGTCATCAT CGTGTTTACT 
GATGGCCAAG CTAACATGGG AGGGAATCCC AAACCTAAAG TGGACCTAAT AAAGAATCTT 
GTCATTAAAA ATAATGCAAG TCGAGAGAAT AAACTTGATC TTTATGTATT TGGAGTTGGA 
AAGGATGTAA AGAAAGAAGA CATGAATGGA TTAGTGTCAG AGAAGAAAGA TGAAAGACAT 
TTTTTTAAGC TTCCAGACTT GGACGAGGTG CAGAATACGT TTGACCTCAT GCTTGATGAT 
AGCACCGTTG TAGGATTGTG TGGGATGCAG CAAAACTATG ATGGATCAAA TAAACGCAGC 
GCATACCCCT GGTTGGCACA GCTTAGTATT GCACAATCGC AGATCTCAGA TTGCATGGGC 
TCGTTAGTTA CCTCCCGGTA TATCTTGACA GCAGCCCACT GTTTTAAAGA AGGAGACACA 
CCGGATAAGA TAACGGTGTA TTTGGAAAAA AACACGGATG TAAAAGTGGA AAAGGTTTTC 
ATTCATCCTA ATTATAGCCT CACAGCAAAA CAAAGCATTG GAATAAAGGA ATTTTATGAC 
TTTGATGTCG CACTTCTGCA GCTCAAAACT CCTGTTAAGA TGAGCGTCAA TCTACGGCCA 
ATCTGCCTCC CCTGCACCAA AGAAACCAAT AGAGCACTGA AACTTTCAGA CAGTCAAGGG 
ACTTGTGAAA AACATGAGCA AATATTATTG AGCAATGAGC TTGTGGATGC TGCTTTCACA 
TCTAAAATGG ACATGGAAAA ACGCAGTCCA CGGAAAATAA GGAGGATCAC AGTCAAGCTT 
GGAAAATATC TGGATGCTTG TGTTGAAGAT GCAAAAAAAG CAAAAGGAAT CGAAGTGGCA 
GACGCGACGG AGGCAGTTAC AAAAAATTTC TTGTGCAGTG GTGGTAATCA ACCACAAAGA 
GATGATGTTT CCTGCAAAGG TGAATCAGGT GGAGCCACAC ATGTGGATAA ATATGGCCGA 
CTGATTCAGA TTGGTGTTGT TAGCTGGGGA GTGAAGAACC TCTGCTCAAA AGCGAAACTT 
GATGCAGTTT CGGTTTCAGA CTCCAGAGAT TACCATATCA ATCTTTTAAG ACCAGATATA 
CAGTCATTCC TTAAAAAGCA CCTGGAAAAT GACAAATTAG GACACACTCT TACTTTCTTG 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcriptomic response and perturbation of toxicity pathways in zebrafish larvae after exposure to graphene quantum dots (GQDs).
Journal of hazardous materials357146-158(2018 09)
Deng S,Jia PP,Zhang JH,Junaid M,Niu A,Ma YB,Fu A,Pei DS

Differential transcriptome analysis of zebrafish (Danio rerio) larvae challenged by Vibrio parahaemolyticus.
Journal of fish diseases41(7)1049-1062(2018 Jul)
Zhang Q,Ji C,Ren J,Zhang Q,Dong X,Zu Y,Jia L,Li W

Innate immune system activation in zebrafish and cellular models of Diamond Blackfan Anemia.
Scientific reports8(1)5165(2018 03)
Danilova N,Wilkes M,Bibikova E,Youn MY,Sakamoto KM,Lin S

Re-evaluating the functional landscape of the cardiovascular system during development.
Biology open6(11)1756-1770(2017 Nov)
Takada N,Omae M,Sagawa F,Chi NC,Endo S,Kozawa S,Sato TN

Anti-Infective Effect of Adhesive Probiotic Lactobacillus in Fish is Correlated With Their Spatial Distribution in the Intestinal Tissue.
Scientific reports7(1)13195(2017 10)
He S,Ran C,Qin C,Li S,Zhang H,de Vos WM,Ringø E,Zhou Z

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cfb cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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