FLT4 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	FLT4
Entrez Gene ID	30121
Full Name	fms-related tyrosine kinase 4
Synonyms	VEGFR3,cb1059
General protein information	Preferred Names fms-related tyrosine kinase 4 Names vascular endothelial growth factor receptor 3 expando receptor tyrosine kinase Flt4
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017358976.2	XP_017214465.1	vascular endothelial growth factor receptor 3 isoform X2
XM_009307792.2	XP_009306067.1	vascular endothelial growth factor receptor 3 isoform X1
XM_009307792.3	XP_009306067.1	vascular endothelial growth factor receptor 3 isoform X1
NM_130945.2	NP_571020.2	vascular endothelial growth factor receptor 3 precursor
XM_017358976.1	XP_017214465.1	vascular endothelial growth factor receptor 3 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	FLT4	NP_891555.2
Rattus norvegicus (Norway rat)	Flt4	NP_446104.1
Gallus gallus (chicken)	FLT4	XP_414600.3
Pan troglodytes (chimpanzee)	FLT4	XP_518160.4
Macaca mulatta (Rhesus monkey)	FLT4	XP_001107118.2
Bos taurus (cattle)	FLT4	XP_002688539.2
Danio rerio (zebrafish)	flt4	NP_571020.1
Xenopus tropicalis (tropical clawed frog)	LOC100493801	XP_002932350.2
Canis lupus familiaris (dog)	FLT4	XP_005626341.1
Mus musculus (house mouse)	Flt4	NP_032055.1

Gene Ontology
Bibliography

Related articles in PubMed

Positive Feedback Defines the Timing, Magnitude, and Robustness of Angiogenesis.
Page DJ, Thuret R, Venkatraman L, Takahashi T, Bentley K, Herbert SP
Cell reports27(11)3139-3151.e5(2019 Jun)

Endoglin is a conserved regulator of vasculogenesis in zebrafish - implications for hereditary haemorrhagic telangiectasia.
Zhang D, Zhou F, Zhao X, Liu B, Chen J, Yang J
Bioscience reports39(5)(2019 05)

Essential role of prostaglandin E
Iwasaki R, Tsuge K, Kishimoto K, Hayashi Y, Iwaana T, Hohjoh H, Inazumi T, Kawahara A, Tsuchiya S, Sugimoto Y
Scientific reports9(1)7650(2019 May)

A 3D Atlas of Hematopoietic Stem and Progenitor Cell Expansion by Multi-dimensional RNA-Seq Analysis.
Xue Y, Liu D, Cui G, Ding Y, Ai D, Gao S, Zhang Y, Suo S, Wang X, Lv P, Zhou C, Li Y, Chen X, Peng G, Jing N, Han JJ, Liu F
Cell reports27(5)1567-1578.e5(2019 04)

Atypical Chemokine Receptor 3 Generates Guidance Cues for CXCL12-Mediated Endothelial Cell Migration.
Tobia C, Chiodelli P, Barbieri A, Buraschi S, Ferrari E, Mitola S, Borsani G, Guerra J, Presta M
Frontiers in immunology101092(2019)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following FLT4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FLT4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa19473	NM_130945.2 Latest version!	Danio rerio fms related receptor tyrosine kinase 4 (flt4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa39161	XM_009307792.3 Latest version!	Danio rerio fms-related tyrosine kinase 4 (flt4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa54884	XM_017358976.2 Latest version!	Danio rerio fms-related tyrosine kinase 4 (flt4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
ODa39161	XM_009307792.2	Danio rerio fms-related tyrosine kinase 4 (flt4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa54884	XM_017358976.1	Danio rerio fms-related tyrosine kinase 4 (flt4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FLT4 ( NM_130945.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_571020.2 Danio rerio fms related receptor tyrosine kinase 4 (flt4), mRNA.

Price & Availability↑

CloneID	ODa19473
Clone ID Related Accession (Same CDS sequence)	NM_130945.2
Accession Version	NM_130945.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576857600000
Organism	Danio rerio(zebrafish)
Product	vascular endothelial growth factor receptor 3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR627495.19 and CR381700.6. On or before Aug 25, 2015 this sequence version replaced XM_005157190.2, NM_130945.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GDQH01015771.1, GFIL01010681.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGAGAG ATTTTACGTT TTTCTGTCGG ATTTGGATTG GGATTCCCTT CTTCTCAGGT 
CTGGTGAATG GGTTTTCTAT GAGTCCACCC ACCCTTGACA ACACCAAAGA CCAGCTTGTG 
ATTAACGCTA ACGACACACT GAACATTACA TGCAGGGGTC AGCGAATTCT GGACTGGAGC 
TGGCCTGAAG AGTCTTTGAG TAAAGTGGAG TTTACTGATC GCCAGGGTCA GCAGTCACCC 
ACTGACACCC CAGGGTATAG AGAAATCAGG CTGAAGGAGT GTCAAGGGGT GGCTGGAAAA 
CCCTACTGCA AGATCTTAAT ACTTACCAAT GCCCAGGCCA ATGACTCGGG TTATTACCGC 
TGCTTCTACA AGGACATTAA AGCTGTTATT GATGGCACCA CGGCAGCCAG CATTTTTGTG 
TTTGTTCGAG ACCCAGAGCA TCCATTCATC AAAAGGGGAG ACAACGACAT GGAGACCATC 
TTCATAACAG ACTCTGAAAC ACACATCGAA GTACCATGTC TGGTTTCAGA TCCTGACCTA 
AAAGTCACTC TCTTCTCGTT AGTGCCGTAT CCAGAGCCTG TGGATGGCAG TGTGGTCACC 
TGGAATAATA AAAAGGGTTG GTCGATTCCC AGGCATATCA TTCAGAACAC TTCCACCTTT 
ATTGGCTTCT ACTGCTCCAT CTCAGTCCAA AACAGCCAGC ACACATCATC AATCTATGTT 
GTCCAGGTCA TTGGGCTGAA GTTTTATGAG TTCAAGCTGT TTCCTGAAGA CTCGCCTGTG 
GAGTTGATGC AGGGGGAGAG TTTGGTGCTC AACTGCACTG CACTGGTCGA CTTCAACACT 
GGTGTGGATT TCCAGTGGGA CTATCCAGGC AAAAAGGAGA ACCGGTTGGC GAGTTTACAG 
CCTCTGCGTA ACGTCCTGGA TGAAGCCACG GAAATCTCCA GTATCCTCTC AATCAGAAAC 
ATTCACTTGG ATGATAGTGG CTACTACACC TGCTGGGCTA ATACTCTGGA AATGAAACGA 
GAGCTCACTA CTGTAGTCAT AGTTCATGAA AAACCTTTTA TCAGCTTGGA CTACAGGAAT 
GGATCAGTTA TAGAGGCAAA AGAGGGACAA AAATCTGTCC GGCTATCCGT AAAAGTGTCA 
GCATACCCTT CACCAGAAAT ACAATGGTAT AAGAATGGAA AACTGATCAG CAGCAAGAAT 
TCAAGCAGGT TTAAGGTCCA GCAGCACTCT CTACAGATCA GGGATGTGTG TAAGCAGGAT 
GCTGGAGAAT ACATGCTGGT CCTGAAAAAT AGCCCTGCAG CCCTGGAGAA AAGACTCAAC 
TTTACTCTCA TAGTCAACGT TCCACCTCAG ATTCATGAGA AGGAGGCAGC ACCACCTACA 
AACCTCTATG GGAAGGGCAC TCGACAGATC CTCACTTGCA CCGCAGACGG GAGCCCTCCA 
GCCTCCATCA GCTGGCAGTG GAGGCCCTGG AGCCCCTGCG ATCTGGAGCG TACCCGCAGA 
GCCCTGAGAC GGAGGGGTGG ACGAGACCAG AGTCCTTTCT GTCACAACTG GATGGATTTG 
GACCCTGAAC ATGCTGTAAA CCCTATTGAG AGTATTGACA CATTGACTCA GATGGTGGAC 
GGCAAAGAAA AGACTGTCGG CCGTGTGGTT ATTCAGAATG CCAGTGTGCC AGCTATGTAC 
AAGTGCTTGG CTGAGAACAA AGTGGGAAAA GATGAACGAC TGATTTATTT CTACGTAACC 
ACTATCCCTG AAGGATTCGA TATAGAGATG GAGCCCTCAG AGGATCCGCT TGAGCAGGAC 
CTGGTACAGC TGAAGTGTAA TGCAGATAAT TTCACCTATG AGAACCTGCG GTGGTACCGC 
CTGGACCCGC AGACTGTTCC TCCAGAGCTG GACTGCAAGA GTCTGCACCA GTATGCCACA 
TTTTTGGAGG GACAACTATC TTTTCAGACC ACCAGCAACA ACTGGGTCCT GCAGCTTAAC 
ATTACCAACA TTCAGCTACA GGATGAGGGG AACTATGTGT GTGAGGTGCA GAACCGACGA 
ACCGGGGTGA AACATTGTCA CCGCAAATAC ATCCCAGTCA AAGCAATGGA AGCACCTCGA 
TACCGTCACA ACCCAACTAA TCATACGGTA AATGTGAGCG AGTCGCTGCA GATGAACTGT 
GATGTGGAAG GCACACCCTT TCCTCAGCTG TCCTGGTTTA AAGATAACCA ACCCCTCCAT 
CAGATATCAG GGATCCTCCT TCAGGACTCC AATCGGACTC TCAGCATTCA GCGAGTGCGA 
GAGGAAGACG CCGGTCTGTA CACCTGCTCT GCCTGCAACC AGAAGGGCTG CGTTCAGTCC 
TCCGCCACGG TGTCAGTGAT TGGCTCTGAT GACAAAACCA ATGTAGAAAT AGTGATCCTC 
ATCGGCACAG GAGTCATCGC CATCTTTTTC TGGGTCCTTC TTCTTGTCAT CTTCTGCAAT 
GTCAAGCGAG TGAACCCAGC TGATATCAAG ACAGGCTATC TGTCCATCAT CATGGACCCG 
GGGGAAGTGC CGCTGGAGGA GCAGTGTGAA TACCTGCCGT ACGACTCCAG CCAGTGGGAG 
ATTTCCAGAG ACAGACTGCG CCTCGGTAAG GTGCTCGGCC ATGGAGCTTT CGGGAAGGTG 
ATTGAAGCCT CAATCTTTGG TCATGACAAG AAATCCTCTG CAAACACAGT GGCTGTCAAG 
ATGTTGAAAG AGGGAGCAAC AGCCAGCGAG CACAAAGCCC TGATGTCAGA GCTGAAGATC 
CTCATTCATA TTGGAAACCA TCTCAATGTT GTCAACCTTC TTGGAGCTTG TACCAAACCT 
AACGGTCCTC TCATGGTTAT AGTGGAGTAT TGTAAATATG GAAATCTGTC CAACTTCCTG 
CGTGCCAAGA GGGAGTTTTT TTTACCATAC AGGGATCGTT CTCCTAAAAC CCAAAGTCAA 
GTGCGACGGA TGATAGAAGC AGGTCAGGCG AGCCAGAGTG AGCATCAGCC GTCCACTTCC 
TCCACCAACC CTCCACGTGT TACAGTGGAT GATCTCTGGA AAACTCCACT CACAATAGAG 
GATCTGATAT GCTACAGTTT TCAAGTTGCA CGAGGAATGG AGTTTCTGGC ATCTCGTAAG 
TGTATTCATC GAGATCTGGC AGCCCGAAAC ATCCTCCTGT CCGAGAACAA TGTGGTGAAG 
ATCTGTGACT TTGGTTTAGC ACGGGACATC TACAAAGACC CTGACTATGT GCGCAAAGGC 
AATGCCAGGC TGCCGCTGAA GTGGATGGCT CCAGAGAGCA TCTTTGATAA GGTTTACACC 
AGTCAGAGTG ACGTCTGGTC TTTTGGAGTT CTGCTCTGGG AGATTTTCTC ACTAGGAGCG 
TCTCCATACC CCGGCATCCA GATTGATGAA GATTTCTGCA AACGACTCAA AGACGGTACC 
AGGATGAGAG CACCAGACAA TGCGTCCCCT GAAATATATG GCATCATGTT GGCCTGCTGG 
CAGGGCGAGC CCAGGGAGAG ACCCACATTT CCAGCTCTGG TGGAGATACT TGGAGATCTA 
CTGCAGGAAA ACAGTCTACC AGAAATCCCG TTCAATGTGT CTCAGAGCTC AGAGGACGAT 
GGCTTCTCAC AAGCTTCTTC CAGACCACCA TCACAAGAGG AGATCAGATT GGCCTGCAAC 
ACACTGCCAA CACGATATTA CAACTGCGTG CCGTTTGCTG GCTGTGTGAT GGTCGGGCCC 
TCCAGCACAT GTCATTCTCG AGTGAAGACC TTTGAGGAAC TTCCTATGGA AATGACATCA 
CACAAGACAC AGCATGACAG TCAGACAGAC AGTGGGATGG TTTTGGCATC AGATGAACTG 
GAGAGGTTTG AACACAAGCA CAGAGGAGCC ATGTTGACAC ATGCGACGAC GGGCCAAAGC 
ACTGAGCGCC TCATCAGTTG TCCATCGGTG AGCAGCAGCG GCAGCGGTGG TGGTTTGCTC 
CGTCCAGTGT TCTTCACTCA ACTCTCAGGC CAGACCTTCT ACAACAACGA GTACGGTCAT 
CTGTCAGAGG AGGGCGTCAG CGACTACTTC TCCTCCTCAG ACCAGGCCGT TTATAAGGAA 
ACCTGCCCGG CAACCTCCAA CCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571020.2
CDS	100..4206
Translation	MKRDFTFFCRIWIGIPFFSGLVNGFSMSPPTLDNTKDQLVINANDTLNITCRGQRILDWSWPEESLSKVEFTDRQGQQSPTDTPGYREIRLKECQGVAGKPYCKILILTNAQANDSGYYRCFYKDIKAVIDGTTAASIFVFVRDPEHPFIKRGDNDMETIFITDSETHIEVPCLVSDPDLKVTLFSLVPYPEPVDGSVVTWNNKKGWSIPRHIIQNTSTFIGFYCSISVQNSQHTSSIYVVQVIGLKFYEFKLFPEDSPVELMQGESLVLNCTALVDFNTGVDFQWDYPGKKENRLASLQPLRNVLDEATEISSILSIRNIHLDDSGYYTCWANTLEMKRELTTVVIVHEKPFISLDYRNGSVIEAKEGQKSVRLSVKVSAYPSPEIQWYKNGKLISSKNSSRFKVQQHSLQIRDVCKQDAGEYMLVLKNSPAALEKRLNFTLIVNVPPQIHEKEAAPPTNLYGKGTRQILTCTADGSPPASISWQWRPWSPCDLERTRRALRRRGGRDQSPFCHNWMDLDPEHAVNPIESIDTLTQMVDGKEKTVGRVVIQNASVPAMYKCLAENKVGKDERLIYFYVTTIPEGFDIEMEPSEDPLEQDLVQLKCNADNFTYENLRWYRLDPQTVPPELDCKSLHQYATFLEGQLSFQTTSNNWVLQLNITNIQLQDEGNYVCEVQNRRTGVKHCHRKYIPVKAMEAPRYRHNPTNHTVNVSESLQMNCDVEGTPFPQLSWFKDNQPLHQISGILLQDSNRTLSIQRVREEDAGLYTCSACNQKGCVQSSATVSVIGSDDKTNVEIVILIGTGVIAIFFWVLLLVIFCNVKRVNPADIKTGYLSIIMDPGEVPLEEQCEYLPYDSSQWEISRDRLRLGKVLGHGAFGKVIEASIFGHDKKSSANTVAVKMLKEGATASEHKALMSELKILIHIGNHLNVVNLLGACTKPNGPLMVIVEYCKYGNLSNFLRAKREFFLPYRDRSPKTQSQVRRMIEAGQASQSEHQPSTSSTNPPRVTVDDLWKTPLTIEDLICYSFQVARGMEFLASRKCIHRDLAARNILLSENNVVKICDFGLARDIYKDPDYVRKGNARLPLKWMAPESIFDKVYTSQSDVWSFGVLLWEIFSLGASPYPGIQIDEDFCKRLKDGTRMRAPDNASPEIYGIMLACWQGEPRERPTFPALVEILGDLLQENSLPEIPFNVSQSSEDDGFSQASSRPPSQEEIRLACNTLPTRYYNCVPFAGCVMVGPSSTCHSRVKTFEELPMEMTSHKTQHDSQTDSGMVLASDELERFEHKHRGAMLTHATTGQSTERLISCPSVSSSGSGGGLLRPVFFTQLSGQTFYNNEYGHLSEEGVSDYFSSSDQAVYKETCPATSNL

Target ORF information:

RefSeq Version	NM_130945.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio fms related receptor tyrosine kinase 4 (flt4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130945.2

ORF Insert Sequence:

ATGAAGAGAG ATTTTACGTT TTTCTGTCGG ATTTGGATTG GGATTCCCTT CTTCTCAGGT 
CTGGTGAATG GGTTTTCTAT GAGTCCACCC ACCCTTGACA ACACCAAAGA CCAGCTTGTG 
ATTAACGCTA ACGACACACT GAACATTACA TGCAGGGGTC AGCGAATTCT GGACTGGAGC 
TGGCCTGAAG AGTCTTTGAG TAAAGTGGAG TTTACTGATC GCCAGGGTCA GCAGTCACCC 
ACTGACACCC CAGGGTATAG AGAAATCAGG CTGAAGGAGT GTCAAGGGGT GGCTGGAAAA 
CCCTACTGCA AGATCTTAAT ACTTACCAAT GCCCAGGCCA ATGACTCGGG TTATTACCGC 
TGCTTCTACA AGGACATTAA AGCTGTTATT GATGGCACCA CGGCAGCCAG CATTTTTGTG 
TTTGTTCGAG ACCCAGAGCA TCCATTCATC AAAAGGGGAG ACAACGACAT GGAGACCATC 
TTCATAACAG ACTCTGAAAC ACACATCGAA GTACCATGTC TGGTTTCAGA TCCTGACCTA 
AAAGTCACTC TCTTCTCGTT AGTGCCGTAT CCAGAGCCTG TGGATGGCAG TGTGGTCACC 
TGGAATAATA AAAAGGGTTG GTCGATTCCC AGGCATATCA TTCAGAACAC TTCCACCTTT 
ATTGGCTTCT ACTGCTCCAT CTCAGTCCAA AACAGCCAGC ACACATCATC AATCTATGTT 
GTCCAGGTCA TTGGGCTGAA GTTTTATGAG TTCAAGCTGT TTCCTGAAGA CTCGCCTGTG 
GAGTTGATGC AGGGGGAGAG TTTGGTGCTC AACTGCACTG CACTGGTCGA CTTCAACACT 
GGTGTGGATT TCCAGTGGGA CTATCCAGGC AAAAAGGAGA ACCGGTTGGC GAGTTTACAG 
CCTCTGCGTA ACGTCCTGGA TGAAGCCACG GAAATCTCCA GTATCCTCTC AATCAGAAAC 
ATTCACTTGG ATGATAGTGG CTACTACACC TGCTGGGCTA ATACTCTGGA AATGAAACGA 
GAGCTCACTA CTGTAGTCAT AGTTCATGAA AAACCTTTTA TCAGCTTGGA CTACAGGAAT 
GGATCAGTTA TAGAGGCAAA AGAGGGACAA AAATCTGTCC GGCTATCCGT AAAAGTGTCA 
GCATACCCTT CACCAGAAAT ACAATGGTAT AAGAATGGAA AACTGATCAG CAGCAAGAAT 
TCAAGCAGGT TTAAGGTCCA GCAGCACTCT CTACAGATCA GGGATGTGTG TAAGCAGGAT 
GCTGGAGAAT ACATGCTGGT CCTGAAAAAT AGCCCTGCAG CCCTGGAGAA AAGACTCAAC 
TTTACTCTCA TAGTCAACGT TCCACCTCAG ATTCATGAGA AGGAGGCAGC ACCACCTACA 
AACCTCTATG GGAAGGGCAC TCGACAGATC CTCACTTGCA CCGCAGACGG GAGCCCTCCA 
GCCTCCATCA GCTGGCAGTG GAGGCCCTGG AGCCCCTGCG ATCTGGAGCG TACCCGCAGA 
GCCCTGAGAC GGAGGGGTGG ACGAGACCAG AGTCCTTTCT GTCACAACTG GATGGATTTG 
GACCCTGAAC ATGCTGTAAA CCCTATTGAG AGTATTGACA CATTGACTCA GATGGTGGAC 
GGCAAAGAAA AGACTGTCGG CCGTGTGGTT ATTCAGAATG CCAGTGTGCC AGCTATGTAC 
AAGTGCTTGG CTGAGAACAA AGTGGGAAAA GATGAACGAC TGATTTATTT CTACGTAACC 
ACTATCCCTG AAGGATTCGA TATAGAGATG GAGCCCTCAG AGGATCCGCT TGAGCAGGAC 
CTGGTACAGC TGAAGTGTAA TGCAGATAAT TTCACCTATG AGAACCTGCG GTGGTACCGC 
CTGGACCCGC AGACTGTTCC TCCAGAGCTG GACTGCAAGA GTCTGCACCA GTATGCCACA 
TTTTTGGAGG GACAACTATC TTTTCAGACC ACCAGCAACA ACTGGGTCCT GCAGCTTAAC 
ATTACCAACA TTCAGCTACA GGATGAGGGG AACTATGTGT GTGAGGTGCA GAACCGACGA 
ACCGGGGTGA AACATTGTCA CCGCAAATAC ATCCCAGTCA AAGCAATGGA AGCACCTCGA 
TACCGTCACA ACCCAACTAA TCATACGGTA AATGTGAGCG AGTCGCTGCA GATGAACTGT 
GATGTGGAAG GCACACCCTT TCCTCAGCTG TCCTGGTTTA AAGATAACCA ACCCCTCCAT 
CAGATATCAG GGATCCTCCT TCAGGACTCC AATCGGACTC TCAGCATTCA GCGAGTGCGA 
GAGGAAGACG CCGGTCTGTA CACCTGCTCT GCCTGCAACC AGAAGGGCTG CGTTCAGTCC 
TCCGCCACGG TGTCAGTGAT TGGCTCTGAT GACAAAACCA ATGTAGAAAT AGTGATCCTC 
ATCGGCACAG GAGTCATCGC CATCTTTTTC TGGGTCCTTC TTCTTGTCAT CTTCTGCAAT 
GTCAAGCGAG TGAACCCAGC TGATATCAAG ACAGGCTATC TGTCCATCAT CATGGACCCG 
GGGGAAGTGC CGCTGGAGGA GCAGTGTGAA TACCTGCCGT ACGACTCCAG CCAGTGGGAG 
ATTTCCAGAG ACAGACTGCG CCTCGGTAAG GTGCTCGGCC ATGGAGCTTT CGGGAAGGTG 
ATTGAAGCCT CAATCTTTGG TCATGACAAG AAATCCTCTG CAAACACAGT GGCTGTCAAG 
ATGTTGAAAG AGGGAGCAAC AGCCAGCGAG CACAAAGCCC TGATGTCAGA GCTGAAGATC 
CTCATTCATA TTGGAAACCA TCTCAATGTT GTCAACCTTC TTGGAGCTTG TACCAAACCT 
AACGGTCCTC TCATGGTTAT AGTGGAGTAT TGTAAATATG GAAATCTGTC CAACTTCCTG 
CGTGCCAAGA GGGAGTTTTT TTTACCATAC AGGGATCGTT CTCCTAAAAC CCAAAGTCAA 
GTGCGACGGA TGATAGAAGC AGGTCAGGCG AGCCAGAGTG AGCATCAGCC GTCCACTTCC 
TCCACCAACC CTCCACGTGT TACAGTGGAT GATCTCTGGA AAACTCCACT CACAATAGAG 
GATCTGATAT GCTACAGTTT TCAAGTTGCA CGAGGAATGG AGTTTCTGGC ATCTCGTAAG 
TGTATTCATC GAGATCTGGC AGCCCGAAAC ATCCTCCTGT CCGAGAACAA TGTGGTGAAG 
ATCTGTGACT TTGGTTTAGC ACGGGACATC TACAAAGACC CTGACTATGT GCGCAAAGGC 
AATGCCAGGC TGCCGCTGAA GTGGATGGCT CCAGAGAGCA TCTTTGATAA GGTTTACACC 
AGTCAGAGTG ACGTCTGGTC TTTTGGAGTT CTGCTCTGGG AGATTTTCTC ACTAGGAGCG 
TCTCCATACC CCGGCATCCA GATTGATGAA GATTTCTGCA AACGACTCAA AGACGGTACC 
AGGATGAGAG CACCAGACAA TGCGTCCCCT GAAATATATG GCATCATGTT GGCCTGCTGG 
CAGGGCGAGC CCAGGGAGAG ACCCACATTT CCAGCTCTGG TGGAGATACT TGGAGATCTA 
CTGCAGGAAA ACAGTCTACC AGAAATCCCG TTCAATGTGT CTCAGAGCTC AGAGGACGAT 
GGCTTCTCAC AAGCTTCTTC CAGACCACCA TCACAAGAGG AGATCAGATT GGCCTGCAAC 
ACACTGCCAA CACGATATTA CAACTGCGTG CCGTTTGCTG GCTGTGTGAT GGTCGGGCCC 
TCCAGCACAT GTCATTCTCG AGTGAAGACC TTTGAGGAAC TTCCTATGGA AATGACATCA 
CACAAGACAC AGCATGACAG TCAGACAGAC AGTGGGATGG TTTTGGCATC AGATGAACTG 
GAGAGGTTTG AACACAAGCA CAGAGGAGCC ATGTTGACAC ATGCGACGAC GGGCCAAAGC 
ACTGAGCGCC TCATCAGTTG TCCATCGGTG AGCAGCAGCG GCAGCGGTGG TGGTTTGCTC 
CGTCCAGTGT TCTTCACTCA ACTCTCAGGC CAGACCTTCT ACAACAACGA GTACGGTCAT 
CTGTCAGAGG AGGGCGTCAG CGACTACTTC TCCTCCTCAG ACCAGGCCGT TTATAAGGAA 
ACCTGCCCGG CAACCTCCAA CCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FLT4 ( XM_009307792.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009306067.1 Danio rerio fms-related tyrosine kinase 4 (flt4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa39161
Clone ID Related Accession (Same CDS sequence)	XM_009307792.2 , XM_009307792.3
Accession Version	XM_009307792.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	vascular endothelial growth factor receptor 3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307792.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAGAG ATTTTACGTT TTTCTGTCGG ATTTGGATTG GGATTCCCTT CTTCTCAGGT 
CTGGTGAATG GGTTTTCTAT GAGTCCACCC ACCCTTGACA ACACCAAAGA CCAGCTTGTG 
ATTAACGCTA ACGACACACT GAACATTACA TGCAGGGGTC AGCGAATTCT GGACTGGAGC 
TGGCCTGAAG AGTCTTTGAG TAAAGTGGAG TTTACTGATC GCCAGGGTCA GCAGTCACCC 
ACTGACACCC CAGGGTATAG AGAAATCAGG CTGAAGGAGT GTCAAGGGGT GGCTGGAAAA 
CCCTACTGCA AGATCTTAAT ACTTACCAAT GCCCAGGCCA ATGACTCGGG TTATTACCGC 
TGCTTCTACA AGGACATTAA AGCTGTTATT GATGGCACCA CGGCAGCCAG CATTTTTGTG 
TTTGTTCGAG ACCCAGAGCA TCCATTCATC AAAAGGGGAG ACAACGACAT GGAGACCATC 
TTCATAACAG ACTCTGAAAC ACACATCGAA GTACCATGTC TGGTTTCAGA TCCTGACCTA 
AAAGTCACTC TCTTCTCGTT AGTGCCGTAT CCAGAGCCTG TGGATGGCAG TGTGGTCACC 
TGGAATAATA AAAAGGGTTG GTCGATTCCC AGGCATATCA TTCAGAACAC TTCCACCTTT 
ATTGGCTTCT ACTGCTCCAT CTCAGTCCAA AACAGCCAGC ACACATCATC AATCTATGTT 
GTCCAGGTCA TTGGGCTGAA GTTTTATGAG TTCAAGCTGT TTCCTGAAGA CTCGCCTGTG 
GAGTTGATGC AGGGGGAGAG TTTGGTGCTC AACTGCACTG CACTGGTCGA CTTCAACACT 
GGTGTGGATT TCCAGTGGGA CTATCCAGGC AAAAAGGAGA ACCGGTTGGC GAGTTTACAG 
CCTCTGCGTA ACGTCCTGGA TGAAGCCACG GAAATCTCCA GTATCCTCTC AATCAGAAAC 
ATTCACTTGG ATGATAGTGG CTACTACACC TGCTGGGCTA ATACTCTGGA AATGAAACGA 
GAGCTCACTA CTGTAGTCAT AGTTCATGAA AAACCTTTTA TCAGCTTGGA CTACAGGAAT 
GGATCAGTTA TAGAGGCAAA AGAGGGACAA AAATCTGTCC GGCTATCCGT AAAAGTGTCA 
GCATACCCTT CACCAGAAAT ACAATGGTAT AAGAATGGAA AACTGATCAG CAGCAAGAAT 
TCAAGCAGGT TTAAGGTCCA GCAGCACTCT CTACAGATCA GGGATGTGTG TAAGCAGGAT 
GCTGGAGAAT ACATGCTGGT CCTGAAAAAT AGCCCTGCAG CCCTGGAGAA AAGACTCAAC 
TTTACTCTCA TAGTCAACGT TCCACCTCAG ATTCATGAGA AGGAGGCAGC ACCACCTACA 
AACCTCTATG GGAAGGGCAC TCGACAGATC CTCACTTGCA CCGCAGACGG GAGCCCTCCA 
GCCTCCATCA GCTGGCAGTG GAGGCCCTGG AGCCCCTGCG ATCTGGAGCG TACCCGCAGA 
GCCCTGAGAC GGAGGGGTGG ACGAGACCAG AGTCCTTTCT GTCACAACTG GATGGATTTG 
GACCCTGAAC ATGCTGTAAA CCCTATTGAG AGTATTGACA CATTGACTCA GATGGTGGAC 
GGCAAAGAAA AGACTGTCGG CCGTGTGGTT ATTCAGAATG CCAGTGTGCC AGCTATGTAC 
AAGTGCTTGG CTGAGAACAA AGTGGGAAAA GATGAACGAC TGATTTATTT CTACGTAACC 
AGATTCGATA TAGAGATGGA GCCCTCAGAG GATCCGCTTG AGCAGGACCT GGTACAGCTG 
AAGTGTAATG CAGATAATTT CACCTATGAG AACCTGCGGT GGTACCGCCT GGACCCGCAG 
ACTGTTCCTC CAGAGCTGGA CTGCAAGAGT CTGCACCAGT ATGCCACATT TTTGGAGGGA 
CAACTATCTT TTCAGACCAC CAGCAACAAC TGGGTCCTGC AGCTTAACAT TACCAACATT 
CAGCTACAGG ATGAGGGGAA CTATGTGTGT GAGGTGCAGA ACCGACGAAC CGGGGTGAAA 
CATTGTCACC GCAAATACAT CCCAGTCAAA GCAATGGAAG CACCTCGATA CCGTCACAAC 
CCAACTAATC ATACGGTAAA TGTGAGCGAG TCGCTGCAGA TGAACTGTGA TGTGGAAGGC 
ACACCCTTTC CTCAGCTGTC CTGGTTTAAA GATAACCAAC CCCTCCATCA GATATCAGGG 
ATCCTCCTTC AGGACTCCAA TCGGACTCTC AGCATTCAGC GAGTGCGAGA GGAAGACGCC 
GGTCTGTACA CCTGCTCTGC CTGCAACCAG AAGGGCTGCG TTCAGTCCTC CGCCACGGTG 
TCAGTGATTG GCTCTGATGA CAAAACCAAT GTAGAAATAG TGATCCTCAT CGGCACAGGA 
GTCATCGCCA TCTTTTTCTG GGTCCTTCTT CTTGTCATCT TCTGCAATGT CAAGCGAGTG 
AACCCAGCTG ATATCAAGAC AGGCTATCTG TCCATCATCA TGGACCCGGG GGAAGTGCCG 
CTGGAGGAGC AGTGTGAATA CCTGCCGTAC GACTCCAGCC AGTGGGAGAT TTCCAGAGAC 
AGACTGCGCC TCGGTAAGGT GCTCGGCCAT GGAGCTTTCG GGAAGGTGAT TGAAGCCTCA 
ATCTTTGGTC ATGACAAGAA ATCCTCTGCA AACACAGTGG CTGTCAAGAT GTTGAAAGAG 
GGAGCAACAG CCAGCGAGCA CAAAGCCCTG ATGTCAGAGC TGAAGATCCT CATTCATATT 
GGAAACCATC TCAATGTTGT CAACCTTCTT GGAGCTTGTA CCAAACCTAA CGGTCCTCTC 
ATGGTTATAG TGGAGTATTG TAAATATGGA AATCTGTCCA ACTTCCTGCG TGCCAAGAGG 
GAGTTTTTTT TACCATACAG GGATCGTTCT CCTAAAACCC AAAGTCAAGT GCGACGGATG 
ATAGAAGCAG GTCAGGCGAG CCAGAGTGAG CATCAGCCGT CCACTTCCTC CACCAACCCT 
CCACGTGTTA CAGTGGATGA TCTCTGGAAA ACTCCACTCA CAATAGAGGA TCTGATATGC 
TACAGTTTTC AAGTTGCACG AGGAATGGAG TTTCTGGCAT CTCGTAAGTG TATTCATCGA 
GATCTGGCAG CCCGAAACAT CCTCCTGTCC GAGAACAATG TGGTGAAGAT CTGTGACTTT 
GGTTTAGCAC GGGACATCTA CAAAGACCCT GACTATGTGC GCAAAGGCAA TGCCAGGCTG 
CCGCTGAAGT GGATGGCTCC AGAGAGCATC TTTGATAAGG TTTACACCAG TCAGAGTGAC 
GTCTGGTCTT TTGGAGTTCT GCTCTGGGAG ATTTTCTCAC TAGGAGCGTC TCCATACCCC 
GGCATCCAGA TTGATGAAGA TTTCTGCAAA CGACTCAAAG ACGGTACCAG GATGAGAGCA 
CCAGACAATG CGTCCCCTGA AATATATGGC ATCATGTTGG CCTGCTGGCA GGGCGAGCCC 
AGGGAGAGAC CCACATTTCC AGCTCTGGTG GAGATACTTG GAGATCTACT GCAGGAAAAC 
AGTCTACCAG AAATCCCGTT CAATGTGTCT CAGAGCTCAG AGGACGATGG CTTCTCACAA 
GCTTCTTCCA GACCACCATC ACAAGAGGAG ATCAGATTGG CCTGCAACAC ACTGCCAACA 
CGATATTACA ACTGCGTGCC GTTTGCTGGC TGTGTGATGG TCGGGCCCTC CAGCACATGT 
CATTCTCGAG TGAAGACCTT TGAGGAACTT CCTATGGAAA TGACATCACA CAAGACACAG 
CATGACAGTC AGACAGACAG TGGGATGGTT TTGGCATCAG ATGAACTGGA GAGGTTTGAA 
CACAAGCACA GAGGAGCCAT GTTGACACAT GCGACGACGG GCCAAAGCAC TGAGCGCCTC 
ATCAGTTGTC CATCGGTGAG CAGCAGCGGC AGCGGTGGTG GTTTGCTCCG TCCAGTGTTC 
TTCACTCAAC TCTCAGGCCA GACCTTCTAC AACAACGAGT ACGGTCATCT GTCAGAGGAG 
GGCGTCAGCG ACTACTTCTC CTCCTCAGAC CAGGCCGTTT ATAAGGAAAC CTGCCCGGCA 
ACCTCCAACC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009306067.1
CDS	368..4462
Translation	MKRDFTFFCRIWIGIPFFSGLVNGFSMSPPTLDNTKDQLVINANDTLNITCRGQRILDWSWPEESLSKVEFTDRQGQQSPTDTPGYREIRLKECQGVAGKPYCKILILTNAQANDSGYYRCFYKDIKAVIDGTTAASIFVFVRDPEHPFIKRGDNDMETIFITDSETHIEVPCLVSDPDLKVTLFSLVPYPEPVDGSVVTWNNKKGWSIPRHIIQNTSTFIGFYCSISVQNSQHTSSIYVVQVIGLKFYEFKLFPEDSPVELMQGESLVLNCTALVDFNTGVDFQWDYPGKKENRLASLQPLRNVLDEATEISSILSIRNIHLDDSGYYTCWANTLEMKRELTTVVIVHEKPFISLDYRNGSVIEAKEGQKSVRLSVKVSAYPSPEIQWYKNGKLISSKNSSRFKVQQHSLQIRDVCKQDAGEYMLVLKNSPAALEKRLNFTLIVNVPPQIHEKEAAPPTNLYGKGTRQILTCTADGSPPASISWQWRPWSPCDLERTRRALRRRGGRDQSPFCHNWMDLDPEHAVNPIESIDTLTQMVDGKEKTVGRVVIQNASVPAMYKCLAENKVGKDERLIYFYVTRFDIEMEPSEDPLEQDLVQLKCNADNFTYENLRWYRLDPQTVPPELDCKSLHQYATFLEGQLSFQTTSNNWVLQLNITNIQLQDEGNYVCEVQNRRTGVKHCHRKYIPVKAMEAPRYRHNPTNHTVNVSESLQMNCDVEGTPFPQLSWFKDNQPLHQISGILLQDSNRTLSIQRVREEDAGLYTCSACNQKGCVQSSATVSVIGSDDKTNVEIVILIGTGVIAIFFWVLLLVIFCNVKRVNPADIKTGYLSIIMDPGEVPLEEQCEYLPYDSSQWEISRDRLRLGKVLGHGAFGKVIEASIFGHDKKSSANTVAVKMLKEGATASEHKALMSELKILIHIGNHLNVVNLLGACTKPNGPLMVIVEYCKYGNLSNFLRAKREFFLPYRDRSPKTQSQVRRMIEAGQASQSEHQPSTSSTNPPRVTVDDLWKTPLTIEDLICYSFQVARGMEFLASRKCIHRDLAARNILLSENNVVKICDFGLARDIYKDPDYVRKGNARLPLKWMAPESIFDKVYTSQSDVWSFGVLLWEIFSLGASPYPGIQIDEDFCKRLKDGTRMRAPDNASPEIYGIMLACWQGEPRERPTFPALVEILGDLLQENSLPEIPFNVSQSSEDDGFSQASSRPPSQEEIRLACNTLPTRYYNCVPFAGCVMVGPSSTCHSRVKTFEELPMEMTSHKTQHDSQTDSGMVLASDELERFEHKHRGAMLTHATTGQSTERLISCPSVSSSGSGGGLLRPVFFTQLSGQTFYNNEYGHLSEEGVSDYFSSSDQAVYKETCPATSNL

Target ORF information:

RefSeq Version	XM_009307792.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio fms-related tyrosine kinase 4 (flt4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307792.2

ORF Insert Sequence:

ATGAAGAGAG ATTTTACGTT TTTCTGTCGG ATTTGGATTG GGATTCCCTT CTTCTCAGGT 
CTGGTGAATG GGTTTTCTAT GAGTCCACCC ACCCTTGACA ACACCAAAGA CCAGCTTGTG 
ATTAACGCTA ACGACACACT GAACATTACA TGCAGGGGTC AGCGAATTCT GGACTGGAGC 
TGGCCTGAAG AGTCTTTGAG TAAAGTGGAG TTTACTGATC GCCAGGGTCA GCAGTCACCC 
ACTGACACCC CAGGGTATAG AGAAATCAGG CTGAAGGAGT GTCAAGGGGT GGCTGGAAAA 
CCCTACTGCA AGATCTTAAT ACTTACCAAT GCCCAGGCCA ATGACTCGGG TTATTACCGC 
TGCTTCTACA AGGACATTAA AGCTGTTATT GATGGCACCA CGGCAGCCAG CATTTTTGTG 
TTTGTTCGAG ACCCAGAGCA TCCATTCATC AAAAGGGGAG ACAACGACAT GGAGACCATC 
TTCATAACAG ACTCTGAAAC ACACATCGAA GTACCATGTC TGGTTTCAGA TCCTGACCTA 
AAAGTCACTC TCTTCTCGTT AGTGCCGTAT CCAGAGCCTG TGGATGGCAG TGTGGTCACC 
TGGAATAATA AAAAGGGTTG GTCGATTCCC AGGCATATCA TTCAGAACAC TTCCACCTTT 
ATTGGCTTCT ACTGCTCCAT CTCAGTCCAA AACAGCCAGC ACACATCATC AATCTATGTT 
GTCCAGGTCA TTGGGCTGAA GTTTTATGAG TTCAAGCTGT TTCCTGAAGA CTCGCCTGTG 
GAGTTGATGC AGGGGGAGAG TTTGGTGCTC AACTGCACTG CACTGGTCGA CTTCAACACT 
GGTGTGGATT TCCAGTGGGA CTATCCAGGC AAAAAGGAGA ACCGGTTGGC GAGTTTACAG 
CCTCTGCGTA ACGTCCTGGA TGAAGCCACG GAAATCTCCA GTATCCTCTC AATCAGAAAC 
ATTCACTTGG ATGATAGTGG CTACTACACC TGCTGGGCTA ATACTCTGGA AATGAAACGA 
GAGCTCACTA CTGTAGTCAT AGTTCATGAA AAACCTTTTA TCAGCTTGGA CTACAGGAAT 
GGATCAGTTA TAGAGGCAAA AGAGGGACAA AAATCTGTCC GGCTATCCGT AAAAGTGTCA 
GCATACCCTT CACCAGAAAT ACAATGGTAT AAGAATGGAA AACTGATCAG CAGCAAGAAT 
TCAAGCAGGT TTAAGGTCCA GCAGCACTCT CTACAGATCA GGGATGTGTG TAAGCAGGAT 
GCTGGAGAAT ACATGCTGGT CCTGAAAAAT AGCCCTGCAG CCCTGGAGAA AAGACTCAAC 
TTTACTCTCA TAGTCAACGT TCCACCTCAG ATTCATGAGA AGGAGGCAGC ACCACCTACA 
AACCTCTATG GGAAGGGCAC TCGACAGATC CTCACTTGCA CCGCAGACGG GAGCCCTCCA 
GCCTCCATCA GCTGGCAGTG GAGGCCCTGG AGCCCCTGCG ATCTGGAGCG TACCCGCAGA 
GCCCTGAGAC GGAGGGGTGG ACGAGACCAG AGTCCTTTCT GTCACAACTG GATGGATTTG 
GACCCTGAAC ATGCTGTAAA CCCTATTGAG AGTATTGACA CATTGACTCA GATGGTGGAC 
GGCAAAGAAA AGACTGTCGG CCGTGTGGTT ATTCAGAATG CCAGTGTGCC AGCTATGTAC 
AAGTGCTTGG CTGAGAACAA AGTGGGAAAA GATGAACGAC TGATTTATTT CTACGTAACC 
AGATTCGATA TAGAGATGGA GCCCTCAGAG GATCCGCTTG AGCAGGACCT GGTACAGCTG 
AAGTGTAATG CAGATAATTT CACCTATGAG AACCTGCGGT GGTACCGCCT GGACCCGCAG 
ACTGTTCCTC CAGAGCTGGA CTGCAAGAGT CTGCACCAGT ATGCCACATT TTTGGAGGGA 
CAACTATCTT TTCAGACCAC CAGCAACAAC TGGGTCCTGC AGCTTAACAT TACCAACATT 
CAGCTACAGG ATGAGGGGAA CTATGTGTGT GAGGTGCAGA ACCGACGAAC CGGGGTGAAA 
CATTGTCACC GCAAATACAT CCCAGTCAAA GCAATGGAAG CACCTCGATA CCGTCACAAC 
CCAACTAATC ATACGGTAAA TGTGAGCGAG TCGCTGCAGA TGAACTGTGA TGTGGAAGGC 
ACACCCTTTC CTCAGCTGTC CTGGTTTAAA GATAACCAAC CCCTCCATCA GATATCAGGG 
ATCCTCCTTC AGGACTCCAA TCGGACTCTC AGCATTCAGC GAGTGCGAGA GGAAGACGCC 
GGTCTGTACA CCTGCTCTGC CTGCAACCAG AAGGGCTGCG TTCAGTCCTC CGCCACGGTG 
TCAGTGATTG GCTCTGATGA CAAAACCAAT GTAGAAATAG TGATCCTCAT CGGCACAGGA 
GTCATCGCCA TCTTTTTCTG GGTCCTTCTT CTTGTCATCT TCTGCAATGT CAAGCGAGTG 
AACCCAGCTG ATATCAAGAC AGGCTATCTG TCCATCATCA TGGACCCGGG GGAAGTGCCG 
CTGGAGGAGC AGTGTGAATA CCTGCCGTAC GACTCCAGCC AGTGGGAGAT TTCCAGAGAC 
AGACTGCGCC TCGGTAAGGT GCTCGGCCAT GGAGCTTTCG GGAAGGTGAT TGAAGCCTCA 
ATCTTTGGTC ATGACAAGAA ATCCTCTGCA AACACAGTGG CTGTCAAGAT GTTGAAAGAG 
GGAGCAACAG CCAGCGAGCA CAAAGCCCTG ATGTCAGAGC TGAAGATCCT CATTCATATT 
GGAAACCATC TCAATGTTGT CAACCTTCTT GGAGCTTGTA CCAAACCTAA CGGTCCTCTC 
ATGGTTATAG TGGAGTATTG TAAATATGGA AATCTGTCCA ACTTCCTGCG TGCCAAGAGG 
GAGTTTTTTT TACCATACAG GGATCGTTCT CCTAAAACCC AAAGTCAAGT GCGACGGATG 
ATAGAAGCAG GTCAGGCGAG CCAGAGTGAG CATCAGCCGT CCACTTCCTC CACCAACCCT 
CCACGTGTTA CAGTGGATGA TCTCTGGAAA ACTCCACTCA CAATAGAGGA TCTGATATGC 
TACAGTTTTC AAGTTGCACG AGGAATGGAG TTTCTGGCAT CTCGTAAGTG TATTCATCGA 
GATCTGGCAG CCCGAAACAT CCTCCTGTCC GAGAACAATG TGGTGAAGAT CTGTGACTTT 
GGTTTAGCAC GGGACATCTA CAAAGACCCT GACTATGTGC GCAAAGGCAA TGCCAGGCTG 
CCGCTGAAGT GGATGGCTCC AGAGAGCATC TTTGATAAGG TTTACACCAG TCAGAGTGAC 
GTCTGGTCTT TTGGAGTTCT GCTCTGGGAG ATTTTCTCAC TAGGAGCGTC TCCATACCCC 
GGCATCCAGA TTGATGAAGA TTTCTGCAAA CGACTCAAAG ACGGTACCAG GATGAGAGCA 
CCAGACAATG CGTCCCCTGA AATATATGGC ATCATGTTGG CCTGCTGGCA GGGCGAGCCC 
AGGGAGAGAC CCACATTTCC AGCTCTGGTG GAGATACTTG GAGATCTACT GCAGGAAAAC 
AGTCTACCAG AAATCCCGTT CAATGTGTCT CAGAGCTCAG AGGACGATGG CTTCTCACAA 
GCTTCTTCCA GACCACCATC ACAAGAGGAG ATCAGATTGG CCTGCAACAC ACTGCCAACA 
CGATATTACA ACTGCGTGCC GTTTGCTGGC TGTGTGATGG TCGGGCCCTC CAGCACATGT 
CATTCTCGAG TGAAGACCTT TGAGGAACTT CCTATGGAAA TGACATCACA CAAGACACAG 
CATGACAGTC AGACAGACAG TGGGATGGTT TTGGCATCAG ATGAACTGGA GAGGTTTGAA 
CACAAGCACA GAGGAGCCAT GTTGACACAT GCGACGACGG GCCAAAGCAC TGAGCGCCTC 
ATCAGTTGTC CATCGGTGAG CAGCAGCGGC AGCGGTGGTG GTTTGCTCCG TCCAGTGTTC 
TTCACTCAAC TCTCAGGCCA GACCTTCTAC AACAACGAGT ACGGTCATCT GTCAGAGGAG 
GGCGTCAGCG ACTACTTCTC CTCCTCAGAC CAGGCCGTTT ATAAGGAAAC CTGCCCGGCA 
ACCTCCAACC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FLT4 ( XM_009307792.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009306067.1 Danio rerio fms-related tyrosine kinase 4 (flt4), transcript variant X1, mRNA.

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CloneID	ODa39161
Clone ID Related Accession (Same CDS sequence)	XM_009307792.2 , XM_009307792.3
Accession Version	XM_009307792.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	vascular endothelial growth factor receptor 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009307792.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAGAG ATTTTACGTT TTTCTGTCGG ATTTGGATTG GGATTCCCTT CTTCTCAGGT 
CTGGTGAATG GGTTTTCTAT GAGTCCACCC ACCCTTGACA ACACCAAAGA CCAGCTTGTG 
ATTAACGCTA ACGACACACT GAACATTACA TGCAGGGGTC AGCGAATTCT GGACTGGAGC 
TGGCCTGAAG AGTCTTTGAG TAAAGTGGAG TTTACTGATC GCCAGGGTCA GCAGTCACCC 
ACTGACACCC CAGGGTATAG AGAAATCAGG CTGAAGGAGT GTCAAGGGGT GGCTGGAAAA 
CCCTACTGCA AGATCTTAAT ACTTACCAAT GCCCAGGCCA ATGACTCGGG TTATTACCGC 
TGCTTCTACA AGGACATTAA AGCTGTTATT GATGGCACCA CGGCAGCCAG CATTTTTGTG 
TTTGTTCGAG ACCCAGAGCA TCCATTCATC AAAAGGGGAG ACAACGACAT GGAGACCATC 
TTCATAACAG ACTCTGAAAC ACACATCGAA GTACCATGTC TGGTTTCAGA TCCTGACCTA 
AAAGTCACTC TCTTCTCGTT AGTGCCGTAT CCAGAGCCTG TGGATGGCAG TGTGGTCACC 
TGGAATAATA AAAAGGGTTG GTCGATTCCC AGGCATATCA TTCAGAACAC TTCCACCTTT 
ATTGGCTTCT ACTGCTCCAT CTCAGTCCAA AACAGCCAGC ACACATCATC AATCTATGTT 
GTCCAGGTCA TTGGGCTGAA GTTTTATGAG TTCAAGCTGT TTCCTGAAGA CTCGCCTGTG 
GAGTTGATGC AGGGGGAGAG TTTGGTGCTC AACTGCACTG CACTGGTCGA CTTCAACACT 
GGTGTGGATT TCCAGTGGGA CTATCCAGGC AAAAAGGAGA ACCGGTTGGC GAGTTTACAG 
CCTCTGCGTA ACGTCCTGGA TGAAGCCACG GAAATCTCCA GTATCCTCTC AATCAGAAAC 
ATTCACTTGG ATGATAGTGG CTACTACACC TGCTGGGCTA ATACTCTGGA AATGAAACGA 
GAGCTCACTA CTGTAGTCAT AGTTCATGAA AAACCTTTTA TCAGCTTGGA CTACAGGAAT 
GGATCAGTTA TAGAGGCAAA AGAGGGACAA AAATCTGTCC GGCTATCCGT AAAAGTGTCA 
GCATACCCTT CACCAGAAAT ACAATGGTAT AAGAATGGAA AACTGATCAG CAGCAAGAAT 
TCAAGCAGGT TTAAGGTCCA GCAGCACTCT CTACAGATCA GGGATGTGTG TAAGCAGGAT 
GCTGGAGAAT ACATGCTGGT CCTGAAAAAT AGCCCTGCAG CCCTGGAGAA AAGACTCAAC 
TTTACTCTCA TAGTCAACGT TCCACCTCAG ATTCATGAGA AGGAGGCAGC ACCACCTACA 
AACCTCTATG GGAAGGGCAC TCGACAGATC CTCACTTGCA CCGCAGACGG GAGCCCTCCA 
GCCTCCATCA GCTGGCAGTG GAGGCCCTGG AGCCCCTGCG ATCTGGAGCG TACCCGCAGA 
GCCCTGAGAC GGAGGGGTGG ACGAGACCAG AGTCCTTTCT GTCACAACTG GATGGATTTG 
GACCCTGAAC ATGCTGTAAA CCCTATTGAG AGTATTGACA CATTGACTCA GATGGTGGAC 
GGCAAAGAAA AGACTGTCGG CCGTGTGGTT ATTCAGAATG CCAGTGTGCC AGCTATGTAC 
AAGTGCTTGG CTGAGAACAA AGTGGGAAAA GATGAACGAC TGATTTATTT CTACGTAACC 
AGATTCGATA TAGAGATGGA GCCCTCAGAG GATCCGCTTG AGCAGGACCT GGTACAGCTG 
AAGTGTAATG CAGATAATTT CACCTATGAG AACCTGCGGT GGTACCGCCT GGACCCGCAG 
ACTGTTCCTC CAGAGCTGGA CTGCAAGAGT CTGCACCAGT ATGCCACATT TTTGGAGGGA 
CAACTATCTT TTCAGACCAC CAGCAACAAC TGGGTCCTGC AGCTTAACAT TACCAACATT 
CAGCTACAGG ATGAGGGGAA CTATGTGTGT GAGGTGCAGA ACCGACGAAC CGGGGTGAAA 
CATTGTCACC GCAAATACAT CCCAGTCAAA GCAATGGAAG CACCTCGATA CCGTCACAAC 
CCAACTAATC ATACGGTAAA TGTGAGCGAG TCGCTGCAGA TGAACTGTGA TGTGGAAGGC 
ACACCCTTTC CTCAGCTGTC CTGGTTTAAA GATAACCAAC CCCTCCATCA GATATCAGGG 
ATCCTCCTTC AGGACTCCAA TCGGACTCTC AGCATTCAGC GAGTGCGAGA GGAAGACGCC 
GGTCTGTACA CCTGCTCTGC CTGCAACCAG AAGGGCTGCG TTCAGTCCTC CGCCACGGTG 
TCAGTGATTG GCTCTGATGA CAAAACCAAT GTAGAAATAG TGATCCTCAT CGGCACAGGA 
GTCATCGCCA TCTTTTTCTG GGTCCTTCTT CTTGTCATCT TCTGCAATGT CAAGCGAGTG 
AACCCAGCTG ATATCAAGAC AGGCTATCTG TCCATCATCA TGGACCCGGG GGAAGTGCCG 
CTGGAGGAGC AGTGTGAATA CCTGCCGTAC GACTCCAGCC AGTGGGAGAT TTCCAGAGAC 
AGACTGCGCC TCGGTAAGGT GCTCGGCCAT GGAGCTTTCG GGAAGGTGAT TGAAGCCTCA 
ATCTTTGGTC ATGACAAGAA ATCCTCTGCA AACACAGTGG CTGTCAAGAT GTTGAAAGAG 
GGAGCAACAG CCAGCGAGCA CAAAGCCCTG ATGTCAGAGC TGAAGATCCT CATTCATATT 
GGAAACCATC TCAATGTTGT CAACCTTCTT GGAGCTTGTA CCAAACCTAA CGGTCCTCTC 
ATGGTTATAG TGGAGTATTG TAAATATGGA AATCTGTCCA ACTTCCTGCG TGCCAAGAGG 
GAGTTTTTTT TACCATACAG GGATCGTTCT CCTAAAACCC AAAGTCAAGT GCGACGGATG 
ATAGAAGCAG GTCAGGCGAG CCAGAGTGAG CATCAGCCGT CCACTTCCTC CACCAACCCT 
CCACGTGTTA CAGTGGATGA TCTCTGGAAA ACTCCACTCA CAATAGAGGA TCTGATATGC 
TACAGTTTTC AAGTTGCACG AGGAATGGAG TTTCTGGCAT CTCGTAAGTG TATTCATCGA 
GATCTGGCAG CCCGAAACAT CCTCCTGTCC GAGAACAATG TGGTGAAGAT CTGTGACTTT 
GGTTTAGCAC GGGACATCTA CAAAGACCCT GACTATGTGC GCAAAGGCAA TGCCAGGCTG 
CCGCTGAAGT GGATGGCTCC AGAGAGCATC TTTGATAAGG TTTACACCAG TCAGAGTGAC 
GTCTGGTCTT TTGGAGTTCT GCTCTGGGAG ATTTTCTCAC TAGGAGCGTC TCCATACCCC 
GGCATCCAGA TTGATGAAGA TTTCTGCAAA CGACTCAAAG ACGGTACCAG GATGAGAGCA 
CCAGACAATG CGTCCCCTGA AATATATGGC ATCATGTTGG CCTGCTGGCA GGGCGAGCCC 
AGGGAGAGAC CCACATTTCC AGCTCTGGTG GAGATACTTG GAGATCTACT GCAGGAAAAC 
AGTCTACCAG AAATCCCGTT CAATGTGTCT CAGAGCTCAG AGGACGATGG CTTCTCACAA 
GCTTCTTCCA GACCACCATC ACAAGAGGAG ATCAGATTGG CCTGCAACAC ACTGCCAACA 
CGATATTACA ACTGCGTGCC GTTTGCTGGC TGTGTGATGG TCGGGCCCTC CAGCACATGT 
CATTCTCGAG TGAAGACCTT TGAGGAACTT CCTATGGAAA TGACATCACA CAAGACACAG 
CATGACAGTC AGACAGACAG TGGGATGGTT TTGGCATCAG ATGAACTGGA GAGGTTTGAA 
CACAAGCACA GAGGAGCCAT GTTGACACAT GCGACGACGG GCCAAAGCAC TGAGCGCCTC 
ATCAGTTGTC CATCGGTGAG CAGCAGCGGC AGCGGTGGTG GTTTGCTCCG TCCAGTGTTC 
TTCACTCAAC TCTCAGGCCA GACCTTCTAC AACAACGAGT ACGGTCATCT GTCAGAGGAG 
GGCGTCAGCG ACTACTTCTC CTCCTCAGAC CAGGCCGTTT ATAAGGAAAC CTGCCCGGCA 
ACCTCCAACC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009306067.1
CDS	370..4464
Translation	MKRDFTFFCRIWIGIPFFSGLVNGFSMSPPTLDNTKDQLVINANDTLNITCRGQRILDWSWPEESLSKVEFTDRQGQQSPTDTPGYREIRLKECQGVAGKPYCKILILTNAQANDSGYYRCFYKDIKAVIDGTTAASIFVFVRDPEHPFIKRGDNDMETIFITDSETHIEVPCLVSDPDLKVTLFSLVPYPEPVDGSVVTWNNKKGWSIPRHIIQNTSTFIGFYCSISVQNSQHTSSIYVVQVIGLKFYEFKLFPEDSPVELMQGESLVLNCTALVDFNTGVDFQWDYPGKKENRLASLQPLRNVLDEATEISSILSIRNIHLDDSGYYTCWANTLEMKRELTTVVIVHEKPFISLDYRNGSVIEAKEGQKSVRLSVKVSAYPSPEIQWYKNGKLISSKNSSRFKVQQHSLQIRDVCKQDAGEYMLVLKNSPAALEKRLNFTLIVNVPPQIHEKEAAPPTNLYGKGTRQILTCTADGSPPASISWQWRPWSPCDLERTRRALRRRGGRDQSPFCHNWMDLDPEHAVNPIESIDTLTQMVDGKEKTVGRVVIQNASVPAMYKCLAENKVGKDERLIYFYVTRFDIEMEPSEDPLEQDLVQLKCNADNFTYENLRWYRLDPQTVPPELDCKSLHQYATFLEGQLSFQTTSNNWVLQLNITNIQLQDEGNYVCEVQNRRTGVKHCHRKYIPVKAMEAPRYRHNPTNHTVNVSESLQMNCDVEGTPFPQLSWFKDNQPLHQISGILLQDSNRTLSIQRVREEDAGLYTCSACNQKGCVQSSATVSVIGSDDKTNVEIVILIGTGVIAIFFWVLLLVIFCNVKRVNPADIKTGYLSIIMDPGEVPLEEQCEYLPYDSSQWEISRDRLRLGKVLGHGAFGKVIEASIFGHDKKSSANTVAVKMLKEGATASEHKALMSELKILIHIGNHLNVVNLLGACTKPNGPLMVIVEYCKYGNLSNFLRAKREFFLPYRDRSPKTQSQVRRMIEAGQASQSEHQPSTSSTNPPRVTVDDLWKTPLTIEDLICYSFQVARGMEFLASRKCIHRDLAARNILLSENNVVKICDFGLARDIYKDPDYVRKGNARLPLKWMAPESIFDKVYTSQSDVWSFGVLLWEIFSLGASPYPGIQIDEDFCKRLKDGTRMRAPDNASPEIYGIMLACWQGEPRERPTFPALVEILGDLLQENSLPEIPFNVSQSSEDDGFSQASSRPPSQEEIRLACNTLPTRYYNCVPFAGCVMVGPSSTCHSRVKTFEELPMEMTSHKTQHDSQTDSGMVLASDELERFEHKHRGAMLTHATTGQSTERLISCPSVSSSGSGGGLLRPVFFTQLSGQTFYNNEYGHLSEEGVSDYFSSSDQAVYKETCPATSNL

Target ORF information:

RefSeq Version	XM_009307792.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio fms-related tyrosine kinase 4 (flt4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307792.3

ORF Insert Sequence:

ATGAAGAGAG ATTTTACGTT TTTCTGTCGG ATTTGGATTG GGATTCCCTT CTTCTCAGGT 
CTGGTGAATG GGTTTTCTAT GAGTCCACCC ACCCTTGACA ACACCAAAGA CCAGCTTGTG 
ATTAACGCTA ACGACACACT GAACATTACA TGCAGGGGTC AGCGAATTCT GGACTGGAGC 
TGGCCTGAAG AGTCTTTGAG TAAAGTGGAG TTTACTGATC GCCAGGGTCA GCAGTCACCC 
ACTGACACCC CAGGGTATAG AGAAATCAGG CTGAAGGAGT GTCAAGGGGT GGCTGGAAAA 
CCCTACTGCA AGATCTTAAT ACTTACCAAT GCCCAGGCCA ATGACTCGGG TTATTACCGC 
TGCTTCTACA AGGACATTAA AGCTGTTATT GATGGCACCA CGGCAGCCAG CATTTTTGTG 
TTTGTTCGAG ACCCAGAGCA TCCATTCATC AAAAGGGGAG ACAACGACAT GGAGACCATC 
TTCATAACAG ACTCTGAAAC ACACATCGAA GTACCATGTC TGGTTTCAGA TCCTGACCTA 
AAAGTCACTC TCTTCTCGTT AGTGCCGTAT CCAGAGCCTG TGGATGGCAG TGTGGTCACC 
TGGAATAATA AAAAGGGTTG GTCGATTCCC AGGCATATCA TTCAGAACAC TTCCACCTTT 
ATTGGCTTCT ACTGCTCCAT CTCAGTCCAA AACAGCCAGC ACACATCATC AATCTATGTT 
GTCCAGGTCA TTGGGCTGAA GTTTTATGAG TTCAAGCTGT TTCCTGAAGA CTCGCCTGTG 
GAGTTGATGC AGGGGGAGAG TTTGGTGCTC AACTGCACTG CACTGGTCGA CTTCAACACT 
GGTGTGGATT TCCAGTGGGA CTATCCAGGC AAAAAGGAGA ACCGGTTGGC GAGTTTACAG 
CCTCTGCGTA ACGTCCTGGA TGAAGCCACG GAAATCTCCA GTATCCTCTC AATCAGAAAC 
ATTCACTTGG ATGATAGTGG CTACTACACC TGCTGGGCTA ATACTCTGGA AATGAAACGA 
GAGCTCACTA CTGTAGTCAT AGTTCATGAA AAACCTTTTA TCAGCTTGGA CTACAGGAAT 
GGATCAGTTA TAGAGGCAAA AGAGGGACAA AAATCTGTCC GGCTATCCGT AAAAGTGTCA 
GCATACCCTT CACCAGAAAT ACAATGGTAT AAGAATGGAA AACTGATCAG CAGCAAGAAT 
TCAAGCAGGT TTAAGGTCCA GCAGCACTCT CTACAGATCA GGGATGTGTG TAAGCAGGAT 
GCTGGAGAAT ACATGCTGGT CCTGAAAAAT AGCCCTGCAG CCCTGGAGAA AAGACTCAAC 
TTTACTCTCA TAGTCAACGT TCCACCTCAG ATTCATGAGA AGGAGGCAGC ACCACCTACA 
AACCTCTATG GGAAGGGCAC TCGACAGATC CTCACTTGCA CCGCAGACGG GAGCCCTCCA 
GCCTCCATCA GCTGGCAGTG GAGGCCCTGG AGCCCCTGCG ATCTGGAGCG TACCCGCAGA 
GCCCTGAGAC GGAGGGGTGG ACGAGACCAG AGTCCTTTCT GTCACAACTG GATGGATTTG 
GACCCTGAAC ATGCTGTAAA CCCTATTGAG AGTATTGACA CATTGACTCA GATGGTGGAC 
GGCAAAGAAA AGACTGTCGG CCGTGTGGTT ATTCAGAATG CCAGTGTGCC AGCTATGTAC 
AAGTGCTTGG CTGAGAACAA AGTGGGAAAA GATGAACGAC TGATTTATTT CTACGTAACC 
AGATTCGATA TAGAGATGGA GCCCTCAGAG GATCCGCTTG AGCAGGACCT GGTACAGCTG 
AAGTGTAATG CAGATAATTT CACCTATGAG AACCTGCGGT GGTACCGCCT GGACCCGCAG 
ACTGTTCCTC CAGAGCTGGA CTGCAAGAGT CTGCACCAGT ATGCCACATT TTTGGAGGGA 
CAACTATCTT TTCAGACCAC CAGCAACAAC TGGGTCCTGC AGCTTAACAT TACCAACATT 
CAGCTACAGG ATGAGGGGAA CTATGTGTGT GAGGTGCAGA ACCGACGAAC CGGGGTGAAA 
CATTGTCACC GCAAATACAT CCCAGTCAAA GCAATGGAAG CACCTCGATA CCGTCACAAC 
CCAACTAATC ATACGGTAAA TGTGAGCGAG TCGCTGCAGA TGAACTGTGA TGTGGAAGGC 
ACACCCTTTC CTCAGCTGTC CTGGTTTAAA GATAACCAAC CCCTCCATCA GATATCAGGG 
ATCCTCCTTC AGGACTCCAA TCGGACTCTC AGCATTCAGC GAGTGCGAGA GGAAGACGCC 
GGTCTGTACA CCTGCTCTGC CTGCAACCAG AAGGGCTGCG TTCAGTCCTC CGCCACGGTG 
TCAGTGATTG GCTCTGATGA CAAAACCAAT GTAGAAATAG TGATCCTCAT CGGCACAGGA 
GTCATCGCCA TCTTTTTCTG GGTCCTTCTT CTTGTCATCT TCTGCAATGT CAAGCGAGTG 
AACCCAGCTG ATATCAAGAC AGGCTATCTG TCCATCATCA TGGACCCGGG GGAAGTGCCG 
CTGGAGGAGC AGTGTGAATA CCTGCCGTAC GACTCCAGCC AGTGGGAGAT TTCCAGAGAC 
AGACTGCGCC TCGGTAAGGT GCTCGGCCAT GGAGCTTTCG GGAAGGTGAT TGAAGCCTCA 
ATCTTTGGTC ATGACAAGAA ATCCTCTGCA AACACAGTGG CTGTCAAGAT GTTGAAAGAG 
GGAGCAACAG CCAGCGAGCA CAAAGCCCTG ATGTCAGAGC TGAAGATCCT CATTCATATT 
GGAAACCATC TCAATGTTGT CAACCTTCTT GGAGCTTGTA CCAAACCTAA CGGTCCTCTC 
ATGGTTATAG TGGAGTATTG TAAATATGGA AATCTGTCCA ACTTCCTGCG TGCCAAGAGG 
GAGTTTTTTT TACCATACAG GGATCGTTCT CCTAAAACCC AAAGTCAAGT GCGACGGATG 
ATAGAAGCAG GTCAGGCGAG CCAGAGTGAG CATCAGCCGT CCACTTCCTC CACCAACCCT 
CCACGTGTTA CAGTGGATGA TCTCTGGAAA ACTCCACTCA CAATAGAGGA TCTGATATGC 
TACAGTTTTC AAGTTGCACG AGGAATGGAG TTTCTGGCAT CTCGTAAGTG TATTCATCGA 
GATCTGGCAG CCCGAAACAT CCTCCTGTCC GAGAACAATG TGGTGAAGAT CTGTGACTTT 
GGTTTAGCAC GGGACATCTA CAAAGACCCT GACTATGTGC GCAAAGGCAA TGCCAGGCTG 
CCGCTGAAGT GGATGGCTCC AGAGAGCATC TTTGATAAGG TTTACACCAG TCAGAGTGAC 
GTCTGGTCTT TTGGAGTTCT GCTCTGGGAG ATTTTCTCAC TAGGAGCGTC TCCATACCCC 
GGCATCCAGA TTGATGAAGA TTTCTGCAAA CGACTCAAAG ACGGTACCAG GATGAGAGCA 
CCAGACAATG CGTCCCCTGA AATATATGGC ATCATGTTGG CCTGCTGGCA GGGCGAGCCC 
AGGGAGAGAC CCACATTTCC AGCTCTGGTG GAGATACTTG GAGATCTACT GCAGGAAAAC 
AGTCTACCAG AAATCCCGTT CAATGTGTCT CAGAGCTCAG AGGACGATGG CTTCTCACAA 
GCTTCTTCCA GACCACCATC ACAAGAGGAG ATCAGATTGG CCTGCAACAC ACTGCCAACA 
CGATATTACA ACTGCGTGCC GTTTGCTGGC TGTGTGATGG TCGGGCCCTC CAGCACATGT 
CATTCTCGAG TGAAGACCTT TGAGGAACTT CCTATGGAAA TGACATCACA CAAGACACAG 
CATGACAGTC AGACAGACAG TGGGATGGTT TTGGCATCAG ATGAACTGGA GAGGTTTGAA 
CACAAGCACA GAGGAGCCAT GTTGACACAT GCGACGACGG GCCAAAGCAC TGAGCGCCTC 
ATCAGTTGTC CATCGGTGAG CAGCAGCGGC AGCGGTGGTG GTTTGCTCCG TCCAGTGTTC 
TTCACTCAAC TCTCAGGCCA GACCTTCTAC AACAACGAGT ACGGTCATCT GTCAGAGGAG 
GGCGTCAGCG ACTACTTCTC CTCCTCAGAC CAGGCCGTTT ATAAGGAAAC CTGCCCGGCA 
ACCTCCAACC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FLT4 ( XM_017358976.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017214465.1 Danio rerio fms-related tyrosine kinase 4 (flt4), transcript variant X2, mRNA.

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CloneID	ODa54884
Clone ID Related Accession (Same CDS sequence)	XM_017358976.2 , XM_017358976.1
Accession Version	XM_017358976.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3975bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	vascular endothelial growth factor receptor 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358976.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAGAG ATTTTACGTT TTTCTGTCGG ATTTGGATTG GGATTCCCTT CTTCTCAGGT 
CTGGTGAATG GGTTTTCTAT GAGTCCACCC ACCCTTGACA ACACCAAAGA CCAGCTTGTG 
ATTAACGCTA ACGACACACT GAACATTACA TGCAGGGGTC AGCGAATTCT GGACTGGAGC 
TGGCCTGAAG AGTCTTTGAG TAAAGTGGAG TTTACTGATC GCCAGGGTCA GCAGTCACCC 
ACTGACACCC CAGGGTATAG AGAAATCAGG CTGAAGGAGT GTCAAGGGGT GGCTGGAAAA 
CCCTACTGCA AGATCTTAAT ACTTACCAAT GCCCAGGCCA ATGACTCGGG TTATTACCGC 
TGCTTCTACA AGGACATTAA AGCTGTTATT GATGGCACCA CGGCAGCCAG CATTTTTGTG 
TTTGTTCGAG ACCCAGAGCA TCCATTCATC AAAAGGGGAG ACAACGACAT GGAGACCATC 
TTCATAACAG ACTCTGAAAC ACACATCGAA GTACCATGTC TGGTTTCAGA TCCTGACCTA 
AAAGTCACTC TCTTCTCGTT AGTGCCGTAT CCAGAGCCTG TGGATGGCAG TGTGGTCACC 
TGGAATAATA AAAAGGGTTG GTCGATTCCC AGGCATATCA TTCAGAACAC TTCCACCTTT 
ATTGGCTTCT ACTGCTCCAT CTCAGTCCAA AACAGCCAGC ACACATCATC AATCTATGTT 
GTCCAGGTCA TTGGGCTGAA GTTTTATGAG TTCAAGCTGT TTCCTGAAGA CTCGCCTGTG 
GAGTTGATGC AGGGGGAGAG TTTGGTGCTC AACTGCACTG CACTGGTCGA CTTCAACACT 
GGTGTGGATT TCCAGTGGGA CTATCCAGGC AAAAAGGAGA ACCGGTTGGC GAGTTTACAG 
CCTCTGCGTA ACGTCCTGGA TGAAGCCACG GAAATCTCCA GTATCCTCTC AATCAGAAAC 
ATTCACTTGG ATGATAGTGG CTACTACACC TGCTGGGCTA ATACTCTGGA AATGAAACGA 
GAGCTCACTA CTGTAGTCAT AGTTCATGAA AAACCTTTTA TCAGCTTGGA CTACAGGAAT 
GGATCAGTTA TAGAGGCAAA AGAGGGACAA AAATCTGTCC GGCTATCCGT AAAAGTGTCA 
GCATACCCTT CACCAGAAAT ACAATGGTAT AAGAATGGAA AACTGATCAG CAGCAAGAAT 
TCAAGCAGGT TTAAGGTCCA GCAGCACTCT CTACAGATCA GGGATGTGTG TAAGCAGGAT 
GCTGGAGAAT ACATGCTGGT CCTGAAAAAT AGCCCTGCAG CCCTGGAGAA AAGACTCAAC 
TTTACTCTCA TAGTCAACGT TCCACCTCAG ATTCATGAGA AGGAGGCAGC ACCACCTACA 
AACCTCTATG GGAAGGGCAC TCGACAGATC CTCACTTGCA CCGCAGACGG GAGCCCTCCA 
GCCTCCATCA GCTGGCAGTG GAGGCCCTGG AGCCCCTGCG ATCTGGAGCG TACCCGCAGA 
GCCCTGAGAC GGAGGGGTGG ACGAGACCAG AGTCCTTTCT GTCACAACTG GATGGATTTG 
GACCCTGAAC ATGCTGTAAA CCCTATTGAG AGTATTGACA CATTGACTCA GATGGTGGAC 
GGCAAAGAAA AGACTGTCGG CCGTGTGGTT ATTCAGAATG CCAGTGTGCC AGCTATGTAC 
AAGTGCTTGG CTGAGAACAA AGTGGGAAAA GATGAACGAC TGATTTATTT CTACGTAACC 
ACTATCCCTG AAGGATTCGA TATAGAGATG GAGCCCTCAG AGGATCCGCT TGAGCAGGAC 
CTGGTACAGC TGAAGTGTAA TGCAGATAAT TTCACCTATG AGAACCTGCG GTGGTACCGC 
CTGGACCCGC AGACTGTTCC TCCAGAGCTG GACTGCAAGA GTCTGCACCA GTATGCCACA 
TTTTTGGAGG GACAACTATC TTTTCAGACC ACCAGCAACA ACTGGGTCCT GCAGCTTAAC 
ATTACCAACA TTCAGCTACA GGATGAGGGG AACTATGTGT GTGAGGTGCA GAACCGACGA 
ACCGGGGTGA AACATTGTCA CCGCAAATAC ATCCCAGTCA AAGCAATGGA AGCACCTCGA 
TACCGTCACA ACCCAACTAA TCATACGGTA AATGTGAGCG AGTCGCTGCA GATGAACTGT 
GATGTGGAAG GCACACCCTT TCCTCAGCTG TCCTGGTTTA AAGATAACCA ACCCCTCCAT 
CAGATATCAG GCTCTGATGA CAAAACCAAT GTAGAAATAG TGATCCTCAT CGGCACAGGA 
GTCATCGCCA TCTTTTTCTG GGTCCTTCTT CTTGTCATCT TCTGCAATGT CAAGCGAGTG 
AACCCAGCTG ATATCAAGAC AGGCTATCTG TCCATCATCA TGGACCCGGG GGAAGTGCCG 
CTGGAGGAGC AGTGTGAATA CCTGCCGTAC GACTCCAGCC AGTGGGAGAT TTCCAGAGAC 
AGACTGCGCC TCGGTAAGGT GCTCGGCCAT GGAGCTTTCG GGAAGGTGAT TGAAGCCTCA 
ATCTTTGGTC ATGACAAGAA ATCCTCTGCA AACACAGTGG CTGTCAAGAT GTTGAAAGAG 
GGAGCAACAG CCAGCGAGCA CAAAGCCCTG ATGTCAGAGC TGAAGATCCT CATTCATATT 
GGAAACCATC TCAATGTTGT CAACCTTCTT GGAGCTTGTA CCAAACCTAA CGGTCCTCTC 
ATGGTTATAG TGGAGTATTG TAAATATGGA AATCTGTCCA ACTTCCTGCG TGCCAAGAGG 
GAGTTTTTTT TACCATACAG GGATCGTTCT CCTAAAACCC AAAGTCAAGT GCGACGGATG 
ATAGAAGCAG GTCAGGCGAG CCAGAGTGAG CATCAGCCGT CCACTTCCTC CACCAACCCT 
CCACGTGTTA CAGTGGATGA TCTCTGGAAA ACTCCACTCA CAATAGAGGA TCTGATATGC 
TACAGTTTTC AAGTTGCACG AGGAATGGAG TTTCTGGCAT CTCGTAAGTG TATTCATCGA 
GATCTGGCAG CCCGAAACAT CCTCCTGTCC GAGAACAATG TGGTGAAGAT CTGTGACTTT 
GGTTTAGCAC GGGACATCTA CAAAGACCCT GACTATGTGC GCAAAGGCAA TGCCAGGCTG 
CCGCTGAAGT GGATGGCTCC AGAGAGCATC TTTGATAAGG TTTACACCAG TCAGAGTGAC 
GTCTGGTCTT TTGGAGTTCT GCTCTGGGAG ATTTTCTCAC TAGGAGCGTC TCCATACCCC 
GGCATCCAGA TTGATGAAGA TTTCTGCAAA CGACTCAAAG ACGGTACCAG GATGAGAGCA 
CCAGACAATG CGTCCCCTGA AATATATGGC ATCATGTTGG CCTGCTGGCA GGGCGAGCCC 
AGGGAGAGAC CCACATTTCC AGCTCTGGTG GAGATACTTG GAGATCTACT GCAGGAAAAC 
AGTCTACCAG AAATCCCGTT CAATGTGTCT CAGAGCTCAG AGGACGATGG CTTCTCACAA 
GCTTCTTCCA GACCACCATC ACAAGAGGAG ATCAGATTGG CCTGCAACAC ACTGCCAACA 
CGATATTACA ACTGCGTGCC GTTTGCTGGC TGTGTGATGG TCGGGCCCTC CAGCACATGT 
CATTCTCGAG TGAAGACCTT TGAGGAACTT CCTATGGAAA TGACATCACA CAAGACACAG 
CATGACAGTC AGACAGACAG TGGGATGGTT TTGGCATCAG ATGAACTGGA GAGGTTTGAA 
CACAAGCACA GAGGAGCCAT GTTGACACAT GCGACGACGG GCCAAAGCAC TGAGCGCCTC 
ATCAGTTGTC CATCGGTGAG CAGCAGCGGC AGCGGTGGTG GTTTGCTCCG TCCAGTGTTC 
TTCACTCAAC TCTCAGGCCA GACCTTCTAC AACAACGAGT ACGGTCATCT GTCAGAGGAG 
GGCGTCAGCG ACTACTTCTC CTCCTCAGAC CAGGCCGTTT ATAAGGAAAC CTGCCCGGCA 
ACCTCCAACC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214465.1
CDS	370..4344
Translation	MKRDFTFFCRIWIGIPFFSGLVNGFSMSPPTLDNTKDQLVINANDTLNITCRGQRILDWSWPEESLSKVEFTDRQGQQSPTDTPGYREIRLKECQGVAGKPYCKILILTNAQANDSGYYRCFYKDIKAVIDGTTAASIFVFVRDPEHPFIKRGDNDMETIFITDSETHIEVPCLVSDPDLKVTLFSLVPYPEPVDGSVVTWNNKKGWSIPRHIIQNTSTFIGFYCSISVQNSQHTSSIYVVQVIGLKFYEFKLFPEDSPVELMQGESLVLNCTALVDFNTGVDFQWDYPGKKENRLASLQPLRNVLDEATEISSILSIRNIHLDDSGYYTCWANTLEMKRELTTVVIVHEKPFISLDYRNGSVIEAKEGQKSVRLSVKVSAYPSPEIQWYKNGKLISSKNSSRFKVQQHSLQIRDVCKQDAGEYMLVLKNSPAALEKRLNFTLIVNVPPQIHEKEAAPPTNLYGKGTRQILTCTADGSPPASISWQWRPWSPCDLERTRRALRRRGGRDQSPFCHNWMDLDPEHAVNPIESIDTLTQMVDGKEKTVGRVVIQNASVPAMYKCLAENKVGKDERLIYFYVTTIPEGFDIEMEPSEDPLEQDLVQLKCNADNFTYENLRWYRLDPQTVPPELDCKSLHQYATFLEGQLSFQTTSNNWVLQLNITNIQLQDEGNYVCEVQNRRTGVKHCHRKYIPVKAMEAPRYRHNPTNHTVNVSESLQMNCDVEGTPFPQLSWFKDNQPLHQISGSDDKTNVEIVILIGTGVIAIFFWVLLLVIFCNVKRVNPADIKTGYLSIIMDPGEVPLEEQCEYLPYDSSQWEISRDRLRLGKVLGHGAFGKVIEASIFGHDKKSSANTVAVKMLKEGATASEHKALMSELKILIHIGNHLNVVNLLGACTKPNGPLMVIVEYCKYGNLSNFLRAKREFFLPYRDRSPKTQSQVRRMIEAGQASQSEHQPSTSSTNPPRVTVDDLWKTPLTIEDLICYSFQVARGMEFLASRKCIHRDLAARNILLSENNVVKICDFGLARDIYKDPDYVRKGNARLPLKWMAPESIFDKVYTSQSDVWSFGVLLWEIFSLGASPYPGIQIDEDFCKRLKDGTRMRAPDNASPEIYGIMLACWQGEPRERPTFPALVEILGDLLQENSLPEIPFNVSQSSEDDGFSQASSRPPSQEEIRLACNTLPTRYYNCVPFAGCVMVGPSSTCHSRVKTFEELPMEMTSHKTQHDSQTDSGMVLASDELERFEHKHRGAMLTHATTGQSTERLISCPSVSSSGSGGGLLRPVFFTQLSGQTFYNNEYGHLSEEGVSDYFSSSDQAVYKETCPATSNL

Target ORF information:

RefSeq Version	XM_017358976.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio fms-related tyrosine kinase 4 (flt4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358976.2

ORF Insert Sequence:

ATGAAGAGAG ATTTTACGTT TTTCTGTCGG ATTTGGATTG GGATTCCCTT CTTCTCAGGT 
CTGGTGAATG GGTTTTCTAT GAGTCCACCC ACCCTTGACA ACACCAAAGA CCAGCTTGTG 
ATTAACGCTA ACGACACACT GAACATTACA TGCAGGGGTC AGCGAATTCT GGACTGGAGC 
TGGCCTGAAG AGTCTTTGAG TAAAGTGGAG TTTACTGATC GCCAGGGTCA GCAGTCACCC 
ACTGACACCC CAGGGTATAG AGAAATCAGG CTGAAGGAGT GTCAAGGGGT GGCTGGAAAA 
CCCTACTGCA AGATCTTAAT ACTTACCAAT GCCCAGGCCA ATGACTCGGG TTATTACCGC 
TGCTTCTACA AGGACATTAA AGCTGTTATT GATGGCACCA CGGCAGCCAG CATTTTTGTG 
TTTGTTCGAG ACCCAGAGCA TCCATTCATC AAAAGGGGAG ACAACGACAT GGAGACCATC 
TTCATAACAG ACTCTGAAAC ACACATCGAA GTACCATGTC TGGTTTCAGA TCCTGACCTA 
AAAGTCACTC TCTTCTCGTT AGTGCCGTAT CCAGAGCCTG TGGATGGCAG TGTGGTCACC 
TGGAATAATA AAAAGGGTTG GTCGATTCCC AGGCATATCA TTCAGAACAC TTCCACCTTT 
ATTGGCTTCT ACTGCTCCAT CTCAGTCCAA AACAGCCAGC ACACATCATC AATCTATGTT 
GTCCAGGTCA TTGGGCTGAA GTTTTATGAG TTCAAGCTGT TTCCTGAAGA CTCGCCTGTG 
GAGTTGATGC AGGGGGAGAG TTTGGTGCTC AACTGCACTG CACTGGTCGA CTTCAACACT 
GGTGTGGATT TCCAGTGGGA CTATCCAGGC AAAAAGGAGA ACCGGTTGGC GAGTTTACAG 
CCTCTGCGTA ACGTCCTGGA TGAAGCCACG GAAATCTCCA GTATCCTCTC AATCAGAAAC 
ATTCACTTGG ATGATAGTGG CTACTACACC TGCTGGGCTA ATACTCTGGA AATGAAACGA 
GAGCTCACTA CTGTAGTCAT AGTTCATGAA AAACCTTTTA TCAGCTTGGA CTACAGGAAT 
GGATCAGTTA TAGAGGCAAA AGAGGGACAA AAATCTGTCC GGCTATCCGT AAAAGTGTCA 
GCATACCCTT CACCAGAAAT ACAATGGTAT AAGAATGGAA AACTGATCAG CAGCAAGAAT 
TCAAGCAGGT TTAAGGTCCA GCAGCACTCT CTACAGATCA GGGATGTGTG TAAGCAGGAT 
GCTGGAGAAT ACATGCTGGT CCTGAAAAAT AGCCCTGCAG CCCTGGAGAA AAGACTCAAC 
TTTACTCTCA TAGTCAACGT TCCACCTCAG ATTCATGAGA AGGAGGCAGC ACCACCTACA 
AACCTCTATG GGAAGGGCAC TCGACAGATC CTCACTTGCA CCGCAGACGG GAGCCCTCCA 
GCCTCCATCA GCTGGCAGTG GAGGCCCTGG AGCCCCTGCG ATCTGGAGCG TACCCGCAGA 
GCCCTGAGAC GGAGGGGTGG ACGAGACCAG AGTCCTTTCT GTCACAACTG GATGGATTTG 
GACCCTGAAC ATGCTGTAAA CCCTATTGAG AGTATTGACA CATTGACTCA GATGGTGGAC 
GGCAAAGAAA AGACTGTCGG CCGTGTGGTT ATTCAGAATG CCAGTGTGCC AGCTATGTAC 
AAGTGCTTGG CTGAGAACAA AGTGGGAAAA GATGAACGAC TGATTTATTT CTACGTAACC 
ACTATCCCTG AAGGATTCGA TATAGAGATG GAGCCCTCAG AGGATCCGCT TGAGCAGGAC 
CTGGTACAGC TGAAGTGTAA TGCAGATAAT TTCACCTATG AGAACCTGCG GTGGTACCGC 
CTGGACCCGC AGACTGTTCC TCCAGAGCTG GACTGCAAGA GTCTGCACCA GTATGCCACA 
TTTTTGGAGG GACAACTATC TTTTCAGACC ACCAGCAACA ACTGGGTCCT GCAGCTTAAC 
ATTACCAACA TTCAGCTACA GGATGAGGGG AACTATGTGT GTGAGGTGCA GAACCGACGA 
ACCGGGGTGA AACATTGTCA CCGCAAATAC ATCCCAGTCA AAGCAATGGA AGCACCTCGA 
TACCGTCACA ACCCAACTAA TCATACGGTA AATGTGAGCG AGTCGCTGCA GATGAACTGT 
GATGTGGAAG GCACACCCTT TCCTCAGCTG TCCTGGTTTA AAGATAACCA ACCCCTCCAT 
CAGATATCAG GCTCTGATGA CAAAACCAAT GTAGAAATAG TGATCCTCAT CGGCACAGGA 
GTCATCGCCA TCTTTTTCTG GGTCCTTCTT CTTGTCATCT TCTGCAATGT CAAGCGAGTG 
AACCCAGCTG ATATCAAGAC AGGCTATCTG TCCATCATCA TGGACCCGGG GGAAGTGCCG 
CTGGAGGAGC AGTGTGAATA CCTGCCGTAC GACTCCAGCC AGTGGGAGAT TTCCAGAGAC 
AGACTGCGCC TCGGTAAGGT GCTCGGCCAT GGAGCTTTCG GGAAGGTGAT TGAAGCCTCA 
ATCTTTGGTC ATGACAAGAA ATCCTCTGCA AACACAGTGG CTGTCAAGAT GTTGAAAGAG 
GGAGCAACAG CCAGCGAGCA CAAAGCCCTG ATGTCAGAGC TGAAGATCCT CATTCATATT 
GGAAACCATC TCAATGTTGT CAACCTTCTT GGAGCTTGTA CCAAACCTAA CGGTCCTCTC 
ATGGTTATAG TGGAGTATTG TAAATATGGA AATCTGTCCA ACTTCCTGCG TGCCAAGAGG 
GAGTTTTTTT TACCATACAG GGATCGTTCT CCTAAAACCC AAAGTCAAGT GCGACGGATG 
ATAGAAGCAG GTCAGGCGAG CCAGAGTGAG CATCAGCCGT CCACTTCCTC CACCAACCCT 
CCACGTGTTA CAGTGGATGA TCTCTGGAAA ACTCCACTCA CAATAGAGGA TCTGATATGC 
TACAGTTTTC AAGTTGCACG AGGAATGGAG TTTCTGGCAT CTCGTAAGTG TATTCATCGA 
GATCTGGCAG CCCGAAACAT CCTCCTGTCC GAGAACAATG TGGTGAAGAT CTGTGACTTT 
GGTTTAGCAC GGGACATCTA CAAAGACCCT GACTATGTGC GCAAAGGCAA TGCCAGGCTG 
CCGCTGAAGT GGATGGCTCC AGAGAGCATC TTTGATAAGG TTTACACCAG TCAGAGTGAC 
GTCTGGTCTT TTGGAGTTCT GCTCTGGGAG ATTTTCTCAC TAGGAGCGTC TCCATACCCC 
GGCATCCAGA TTGATGAAGA TTTCTGCAAA CGACTCAAAG ACGGTACCAG GATGAGAGCA 
CCAGACAATG CGTCCCCTGA AATATATGGC ATCATGTTGG CCTGCTGGCA GGGCGAGCCC 
AGGGAGAGAC CCACATTTCC AGCTCTGGTG GAGATACTTG GAGATCTACT GCAGGAAAAC 
AGTCTACCAG AAATCCCGTT CAATGTGTCT CAGAGCTCAG AGGACGATGG CTTCTCACAA 
GCTTCTTCCA GACCACCATC ACAAGAGGAG ATCAGATTGG CCTGCAACAC ACTGCCAACA 
CGATATTACA ACTGCGTGCC GTTTGCTGGC TGTGTGATGG TCGGGCCCTC CAGCACATGT 
CATTCTCGAG TGAAGACCTT TGAGGAACTT CCTATGGAAA TGACATCACA CAAGACACAG 
CATGACAGTC AGACAGACAG TGGGATGGTT TTGGCATCAG ATGAACTGGA GAGGTTTGAA 
CACAAGCACA GAGGAGCCAT GTTGACACAT GCGACGACGG GCCAAAGCAC TGAGCGCCTC 
ATCAGTTGTC CATCGGTGAG CAGCAGCGGC AGCGGTGGTG GTTTGCTCCG TCCAGTGTTC 
TTCACTCAAC TCTCAGGCCA GACCTTCTAC AACAACGAGT ACGGTCATCT GTCAGAGGAG 
GGCGTCAGCG ACTACTTCTC CTCCTCAGAC CAGGCCGTTT ATAAGGAAAC CTGCCCGGCA 
ACCTCCAACC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FLT4 ( XM_017358976.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017214465.1 Danio rerio fms-related tyrosine kinase 4 (flt4), transcript variant X2, mRNA.

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CloneID	ODa54884
Clone ID Related Accession (Same CDS sequence)	XM_017358976.2 , XM_017358976.1
Accession Version	XM_017358976.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3975bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	vascular endothelial growth factor receptor 3 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAGAG ATTTTACGTT TTTCTGTCGG ATTTGGATTG GGATTCCCTT CTTCTCAGGT 
CTGGTGAATG GGTTTTCTAT GAGTCCACCC ACCCTTGACA ACACCAAAGA CCAGCTTGTG 
ATTAACGCTA ACGACACACT GAACATTACA TGCAGGGGTC AGCGAATTCT GGACTGGAGC 
TGGCCTGAAG AGTCTTTGAG TAAAGTGGAG TTTACTGATC GCCAGGGTCA GCAGTCACCC 
ACTGACACCC CAGGGTATAG AGAAATCAGG CTGAAGGAGT GTCAAGGGGT GGCTGGAAAA 
CCCTACTGCA AGATCTTAAT ACTTACCAAT GCCCAGGCCA ATGACTCGGG TTATTACCGC 
TGCTTCTACA AGGACATTAA AGCTGTTATT GATGGCACCA CGGCAGCCAG CATTTTTGTG 
TTTGTTCGAG ACCCAGAGCA TCCATTCATC AAAAGGGGAG ACAACGACAT GGAGACCATC 
TTCATAACAG ACTCTGAAAC ACACATCGAA GTACCATGTC TGGTTTCAGA TCCTGACCTA 
AAAGTCACTC TCTTCTCGTT AGTGCCGTAT CCAGAGCCTG TGGATGGCAG TGTGGTCACC 
TGGAATAATA AAAAGGGTTG GTCGATTCCC AGGCATATCA TTCAGAACAC TTCCACCTTT 
ATTGGCTTCT ACTGCTCCAT CTCAGTCCAA AACAGCCAGC ACACATCATC AATCTATGTT 
GTCCAGGTCA TTGGGCTGAA GTTTTATGAG TTCAAGCTGT TTCCTGAAGA CTCGCCTGTG 
GAGTTGATGC AGGGGGAGAG TTTGGTGCTC AACTGCACTG CACTGGTCGA CTTCAACACT 
GGTGTGGATT TCCAGTGGGA CTATCCAGGC AAAAAGGAGA ACCGGTTGGC GAGTTTACAG 
CCTCTGCGTA ACGTCCTGGA TGAAGCCACG GAAATCTCCA GTATCCTCTC AATCAGAAAC 
ATTCACTTGG ATGATAGTGG CTACTACACC TGCTGGGCTA ATACTCTGGA AATGAAACGA 
GAGCTCACTA CTGTAGTCAT AGTTCATGAA AAACCTTTTA TCAGCTTGGA CTACAGGAAT 
GGATCAGTTA TAGAGGCAAA AGAGGGACAA AAATCTGTCC GGCTATCCGT AAAAGTGTCA 
GCATACCCTT CACCAGAAAT ACAATGGTAT AAGAATGGAA AACTGATCAG CAGCAAGAAT 
TCAAGCAGGT TTAAGGTCCA GCAGCACTCT CTACAGATCA GGGATGTGTG TAAGCAGGAT 
GCTGGAGAAT ACATGCTGGT CCTGAAAAAT AGCCCTGCAG CCCTGGAGAA AAGACTCAAC 
TTTACTCTCA TAGTCAACGT TCCACCTCAG ATTCATGAGA AGGAGGCAGC ACCACCTACA 
AACCTCTATG GGAAGGGCAC TCGACAGATC CTCACTTGCA CCGCAGACGG GAGCCCTCCA 
GCCTCCATCA GCTGGCAGTG GAGGCCCTGG AGCCCCTGCG ATCTGGAGCG TACCCGCAGA 
GCCCTGAGAC GGAGGGGTGG ACGAGACCAG AGTCCTTTCT GTCACAACTG GATGGATTTG 
GACCCTGAAC ATGCTGTAAA CCCTATTGAG AGTATTGACA CATTGACTCA GATGGTGGAC 
GGCAAAGAAA AGACTGTCGG CCGTGTGGTT ATTCAGAATG CCAGTGTGCC AGCTATGTAC 
AAGTGCTTGG CTGAGAACAA AGTGGGAAAA GATGAACGAC TGATTTATTT CTACGTAACC 
ACTATCCCTG AAGGATTCGA TATAGAGATG GAGCCCTCAG AGGATCCGCT TGAGCAGGAC 
CTGGTACAGC TGAAGTGTAA TGCAGATAAT TTCACCTATG AGAACCTGCG GTGGTACCGC 
CTGGACCCGC AGACTGTTCC TCCAGAGCTG GACTGCAAGA GTCTGCACCA GTATGCCACA 
TTTTTGGAGG GACAACTATC TTTTCAGACC ACCAGCAACA ACTGGGTCCT GCAGCTTAAC 
ATTACCAACA TTCAGCTACA GGATGAGGGG AACTATGTGT GTGAGGTGCA GAACCGACGA 
ACCGGGGTGA AACATTGTCA CCGCAAATAC ATCCCAGTCA AAGCAATGGA AGCACCTCGA 
TACCGTCACA ACCCAACTAA TCATACGGTA AATGTGAGCG AGTCGCTGCA GATGAACTGT 
GATGTGGAAG GCACACCCTT TCCTCAGCTG TCCTGGTTTA AAGATAACCA ACCCCTCCAT 
CAGATATCAG GCTCTGATGA CAAAACCAAT GTAGAAATAG TGATCCTCAT CGGCACAGGA 
GTCATCGCCA TCTTTTTCTG GGTCCTTCTT CTTGTCATCT TCTGCAATGT CAAGCGAGTG 
AACCCAGCTG ATATCAAGAC AGGCTATCTG TCCATCATCA TGGACCCGGG GGAAGTGCCG 
CTGGAGGAGC AGTGTGAATA CCTGCCGTAC GACTCCAGCC AGTGGGAGAT TTCCAGAGAC 
AGACTGCGCC TCGGTAAGGT GCTCGGCCAT GGAGCTTTCG GGAAGGTGAT TGAAGCCTCA 
ATCTTTGGTC ATGACAAGAA ATCCTCTGCA AACACAGTGG CTGTCAAGAT GTTGAAAGAG 
GGAGCAACAG CCAGCGAGCA CAAAGCCCTG ATGTCAGAGC TGAAGATCCT CATTCATATT 
GGAAACCATC TCAATGTTGT CAACCTTCTT GGAGCTTGTA CCAAACCTAA CGGTCCTCTC 
ATGGTTATAG TGGAGTATTG TAAATATGGA AATCTGTCCA ACTTCCTGCG TGCCAAGAGG 
GAGTTTTTTT TACCATACAG GGATCGTTCT CCTAAAACCC AAAGTCAAGT GCGACGGATG 
ATAGAAGCAG GTCAGGCGAG CCAGAGTGAG CATCAGCCGT CCACTTCCTC CACCAACCCT 
CCACGTGTTA CAGTGGATGA TCTCTGGAAA ACTCCACTCA CAATAGAGGA TCTGATATGC 
TACAGTTTTC AAGTTGCACG AGGAATGGAG TTTCTGGCAT CTCGTAAGTG TATTCATCGA 
GATCTGGCAG CCCGAAACAT CCTCCTGTCC GAGAACAATG TGGTGAAGAT CTGTGACTTT 
GGTTTAGCAC GGGACATCTA CAAAGACCCT GACTATGTGC GCAAAGGCAA TGCCAGGCTG 
CCGCTGAAGT GGATGGCTCC AGAGAGCATC TTTGATAAGG TTTACACCAG TCAGAGTGAC 
GTCTGGTCTT TTGGAGTTCT GCTCTGGGAG ATTTTCTCAC TAGGAGCGTC TCCATACCCC 
GGCATCCAGA TTGATGAAGA TTTCTGCAAA CGACTCAAAG ACGGTACCAG GATGAGAGCA 
CCAGACAATG CGTCCCCTGA AATATATGGC ATCATGTTGG CCTGCTGGCA GGGCGAGCCC 
AGGGAGAGAC CCACATTTCC AGCTCTGGTG GAGATACTTG GAGATCTACT GCAGGAAAAC 
AGTCTACCAG AAATCCCGTT CAATGTGTCT CAGAGCTCAG AGGACGATGG CTTCTCACAA 
GCTTCTTCCA GACCACCATC ACAAGAGGAG ATCAGATTGG CCTGCAACAC ACTGCCAACA 
CGATATTACA ACTGCGTGCC GTTTGCTGGC TGTGTGATGG TCGGGCCCTC CAGCACATGT 
CATTCTCGAG TGAAGACCTT TGAGGAACTT CCTATGGAAA TGACATCACA CAAGACACAG 
CATGACAGTC AGACAGACAG TGGGATGGTT TTGGCATCAG ATGAACTGGA GAGGTTTGAA 
CACAAGCACA GAGGAGCCAT GTTGACACAT GCGACGACGG GCCAAAGCAC TGAGCGCCTC 
ATCAGTTGTC CATCGGTGAG CAGCAGCGGC AGCGGTGGTG GTTTGCTCCG TCCAGTGTTC 
TTCACTCAAC TCTCAGGCCA GACCTTCTAC AACAACGAGT ACGGTCATCT GTCAGAGGAG 
GGCGTCAGCG ACTACTTCTC CTCCTCAGAC CAGGCCGTTT ATAAGGAAAC CTGCCCGGCA 
ACCTCCAACC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214465.1
CDS	368..4342
Translation	MKRDFTFFCRIWIGIPFFSGLVNGFSMSPPTLDNTKDQLVINANDTLNITCRGQRILDWSWPEESLSKVEFTDRQGQQSPTDTPGYREIRLKECQGVAGKPYCKILILTNAQANDSGYYRCFYKDIKAVIDGTTAASIFVFVRDPEHPFIKRGDNDMETIFITDSETHIEVPCLVSDPDLKVTLFSLVPYPEPVDGSVVTWNNKKGWSIPRHIIQNTSTFIGFYCSISVQNSQHTSSIYVVQVIGLKFYEFKLFPEDSPVELMQGESLVLNCTALVDFNTGVDFQWDYPGKKENRLASLQPLRNVLDEATEISSILSIRNIHLDDSGYYTCWANTLEMKRELTTVVIVHEKPFISLDYRNGSVIEAKEGQKSVRLSVKVSAYPSPEIQWYKNGKLISSKNSSRFKVQQHSLQIRDVCKQDAGEYMLVLKNSPAALEKRLNFTLIVNVPPQIHEKEAAPPTNLYGKGTRQILTCTADGSPPASISWQWRPWSPCDLERTRRALRRRGGRDQSPFCHNWMDLDPEHAVNPIESIDTLTQMVDGKEKTVGRVVIQNASVPAMYKCLAENKVGKDERLIYFYVTTIPEGFDIEMEPSEDPLEQDLVQLKCNADNFTYENLRWYRLDPQTVPPELDCKSLHQYATFLEGQLSFQTTSNNWVLQLNITNIQLQDEGNYVCEVQNRRTGVKHCHRKYIPVKAMEAPRYRHNPTNHTVNVSESLQMNCDVEGTPFPQLSWFKDNQPLHQISGSDDKTNVEIVILIGTGVIAIFFWVLLLVIFCNVKRVNPADIKTGYLSIIMDPGEVPLEEQCEYLPYDSSQWEISRDRLRLGKVLGHGAFGKVIEASIFGHDKKSSANTVAVKMLKEGATASEHKALMSELKILIHIGNHLNVVNLLGACTKPNGPLMVIVEYCKYGNLSNFLRAKREFFLPYRDRSPKTQSQVRRMIEAGQASQSEHQPSTSSTNPPRVTVDDLWKTPLTIEDLICYSFQVARGMEFLASRKCIHRDLAARNILLSENNVVKICDFGLARDIYKDPDYVRKGNARLPLKWMAPESIFDKVYTSQSDVWSFGVLLWEIFSLGASPYPGIQIDEDFCKRLKDGTRMRAPDNASPEIYGIMLACWQGEPRERPTFPALVEILGDLLQENSLPEIPFNVSQSSEDDGFSQASSRPPSQEEIRLACNTLPTRYYNCVPFAGCVMVGPSSTCHSRVKTFEELPMEMTSHKTQHDSQTDSGMVLASDELERFEHKHRGAMLTHATTGQSTERLISCPSVSSSGSGGGLLRPVFFTQLSGQTFYNNEYGHLSEEGVSDYFSSSDQAVYKETCPATSNL

Target ORF information:

RefSeq Version	XM_017358976.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio fms-related tyrosine kinase 4 (flt4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358976.1

ORF Insert Sequence:

ATGAAGAGAG ATTTTACGTT TTTCTGTCGG ATTTGGATTG GGATTCCCTT CTTCTCAGGT 
CTGGTGAATG GGTTTTCTAT GAGTCCACCC ACCCTTGACA ACACCAAAGA CCAGCTTGTG 
ATTAACGCTA ACGACACACT GAACATTACA TGCAGGGGTC AGCGAATTCT GGACTGGAGC 
TGGCCTGAAG AGTCTTTGAG TAAAGTGGAG TTTACTGATC GCCAGGGTCA GCAGTCACCC 
ACTGACACCC CAGGGTATAG AGAAATCAGG CTGAAGGAGT GTCAAGGGGT GGCTGGAAAA 
CCCTACTGCA AGATCTTAAT ACTTACCAAT GCCCAGGCCA ATGACTCGGG TTATTACCGC 
TGCTTCTACA AGGACATTAA AGCTGTTATT GATGGCACCA CGGCAGCCAG CATTTTTGTG 
TTTGTTCGAG ACCCAGAGCA TCCATTCATC AAAAGGGGAG ACAACGACAT GGAGACCATC 
TTCATAACAG ACTCTGAAAC ACACATCGAA GTACCATGTC TGGTTTCAGA TCCTGACCTA 
AAAGTCACTC TCTTCTCGTT AGTGCCGTAT CCAGAGCCTG TGGATGGCAG TGTGGTCACC 
TGGAATAATA AAAAGGGTTG GTCGATTCCC AGGCATATCA TTCAGAACAC TTCCACCTTT 
ATTGGCTTCT ACTGCTCCAT CTCAGTCCAA AACAGCCAGC ACACATCATC AATCTATGTT 
GTCCAGGTCA TTGGGCTGAA GTTTTATGAG TTCAAGCTGT TTCCTGAAGA CTCGCCTGTG 
GAGTTGATGC AGGGGGAGAG TTTGGTGCTC AACTGCACTG CACTGGTCGA CTTCAACACT 
GGTGTGGATT TCCAGTGGGA CTATCCAGGC AAAAAGGAGA ACCGGTTGGC GAGTTTACAG 
CCTCTGCGTA ACGTCCTGGA TGAAGCCACG GAAATCTCCA GTATCCTCTC AATCAGAAAC 
ATTCACTTGG ATGATAGTGG CTACTACACC TGCTGGGCTA ATACTCTGGA AATGAAACGA 
GAGCTCACTA CTGTAGTCAT AGTTCATGAA AAACCTTTTA TCAGCTTGGA CTACAGGAAT 
GGATCAGTTA TAGAGGCAAA AGAGGGACAA AAATCTGTCC GGCTATCCGT AAAAGTGTCA 
GCATACCCTT CACCAGAAAT ACAATGGTAT AAGAATGGAA AACTGATCAG CAGCAAGAAT 
TCAAGCAGGT TTAAGGTCCA GCAGCACTCT CTACAGATCA GGGATGTGTG TAAGCAGGAT 
GCTGGAGAAT ACATGCTGGT CCTGAAAAAT AGCCCTGCAG CCCTGGAGAA AAGACTCAAC 
TTTACTCTCA TAGTCAACGT TCCACCTCAG ATTCATGAGA AGGAGGCAGC ACCACCTACA 
AACCTCTATG GGAAGGGCAC TCGACAGATC CTCACTTGCA CCGCAGACGG GAGCCCTCCA 
GCCTCCATCA GCTGGCAGTG GAGGCCCTGG AGCCCCTGCG ATCTGGAGCG TACCCGCAGA 
GCCCTGAGAC GGAGGGGTGG ACGAGACCAG AGTCCTTTCT GTCACAACTG GATGGATTTG 
GACCCTGAAC ATGCTGTAAA CCCTATTGAG AGTATTGACA CATTGACTCA GATGGTGGAC 
GGCAAAGAAA AGACTGTCGG CCGTGTGGTT ATTCAGAATG CCAGTGTGCC AGCTATGTAC 
AAGTGCTTGG CTGAGAACAA AGTGGGAAAA GATGAACGAC TGATTTATTT CTACGTAACC 
ACTATCCCTG AAGGATTCGA TATAGAGATG GAGCCCTCAG AGGATCCGCT TGAGCAGGAC 
CTGGTACAGC TGAAGTGTAA TGCAGATAAT TTCACCTATG AGAACCTGCG GTGGTACCGC 
CTGGACCCGC AGACTGTTCC TCCAGAGCTG GACTGCAAGA GTCTGCACCA GTATGCCACA 
TTTTTGGAGG GACAACTATC TTTTCAGACC ACCAGCAACA ACTGGGTCCT GCAGCTTAAC 
ATTACCAACA TTCAGCTACA GGATGAGGGG AACTATGTGT GTGAGGTGCA GAACCGACGA 
ACCGGGGTGA AACATTGTCA CCGCAAATAC ATCCCAGTCA AAGCAATGGA AGCACCTCGA 
TACCGTCACA ACCCAACTAA TCATACGGTA AATGTGAGCG AGTCGCTGCA GATGAACTGT 
GATGTGGAAG GCACACCCTT TCCTCAGCTG TCCTGGTTTA AAGATAACCA ACCCCTCCAT 
CAGATATCAG GCTCTGATGA CAAAACCAAT GTAGAAATAG TGATCCTCAT CGGCACAGGA 
GTCATCGCCA TCTTTTTCTG GGTCCTTCTT CTTGTCATCT TCTGCAATGT CAAGCGAGTG 
AACCCAGCTG ATATCAAGAC AGGCTATCTG TCCATCATCA TGGACCCGGG GGAAGTGCCG 
CTGGAGGAGC AGTGTGAATA CCTGCCGTAC GACTCCAGCC AGTGGGAGAT TTCCAGAGAC 
AGACTGCGCC TCGGTAAGGT GCTCGGCCAT GGAGCTTTCG GGAAGGTGAT TGAAGCCTCA 
ATCTTTGGTC ATGACAAGAA ATCCTCTGCA AACACAGTGG CTGTCAAGAT GTTGAAAGAG 
GGAGCAACAG CCAGCGAGCA CAAAGCCCTG ATGTCAGAGC TGAAGATCCT CATTCATATT 
GGAAACCATC TCAATGTTGT CAACCTTCTT GGAGCTTGTA CCAAACCTAA CGGTCCTCTC 
ATGGTTATAG TGGAGTATTG TAAATATGGA AATCTGTCCA ACTTCCTGCG TGCCAAGAGG 
GAGTTTTTTT TACCATACAG GGATCGTTCT CCTAAAACCC AAAGTCAAGT GCGACGGATG 
ATAGAAGCAG GTCAGGCGAG CCAGAGTGAG CATCAGCCGT CCACTTCCTC CACCAACCCT 
CCACGTGTTA CAGTGGATGA TCTCTGGAAA ACTCCACTCA CAATAGAGGA TCTGATATGC 
TACAGTTTTC AAGTTGCACG AGGAATGGAG TTTCTGGCAT CTCGTAAGTG TATTCATCGA 
GATCTGGCAG CCCGAAACAT CCTCCTGTCC GAGAACAATG TGGTGAAGAT CTGTGACTTT 
GGTTTAGCAC GGGACATCTA CAAAGACCCT GACTATGTGC GCAAAGGCAA TGCCAGGCTG 
CCGCTGAAGT GGATGGCTCC AGAGAGCATC TTTGATAAGG TTTACACCAG TCAGAGTGAC 
GTCTGGTCTT TTGGAGTTCT GCTCTGGGAG ATTTTCTCAC TAGGAGCGTC TCCATACCCC 
GGCATCCAGA TTGATGAAGA TTTCTGCAAA CGACTCAAAG ACGGTACCAG GATGAGAGCA 
CCAGACAATG CGTCCCCTGA AATATATGGC ATCATGTTGG CCTGCTGGCA GGGCGAGCCC 
AGGGAGAGAC CCACATTTCC AGCTCTGGTG GAGATACTTG GAGATCTACT GCAGGAAAAC 
AGTCTACCAG AAATCCCGTT CAATGTGTCT CAGAGCTCAG AGGACGATGG CTTCTCACAA 
GCTTCTTCCA GACCACCATC ACAAGAGGAG ATCAGATTGG CCTGCAACAC ACTGCCAACA 
CGATATTACA ACTGCGTGCC GTTTGCTGGC TGTGTGATGG TCGGGCCCTC CAGCACATGT 
CATTCTCGAG TGAAGACCTT TGAGGAACTT CCTATGGAAA TGACATCACA CAAGACACAG 
CATGACAGTC AGACAGACAG TGGGATGGTT TTGGCATCAG ATGAACTGGA GAGGTTTGAA 
CACAAGCACA GAGGAGCCAT GTTGACACAT GCGACGACGG GCCAAAGCAC TGAGCGCCTC 
ATCAGTTGTC CATCGGTGAG CAGCAGCGGC AGCGGTGGTG GTTTGCTCCG TCCAGTGTTC 
TTCACTCAAC TCTCAGGCCA GACCTTCTAC AACAACGAGT ACGGTCATCT GTCAGAGGAG 
GGCGTCAGCG ACTACTTCTC CTCCTCAGAC CAGGCCGTTT ATAAGGAAAC CTGCCCGGCA 
ACCTCCAACC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Positive Feedback Defines the Timing, Magnitude, and Robustness of Angiogenesis.
Cell reports27(11)3139-3151.e5(2019 Jun)
Page DJ,Thuret R,Venkatraman L,Takahashi T,Bentley K,Herbert SP

Endoglin is a conserved regulator of vasculogenesis in zebrafish - implications for hereditary haemorrhagic telangiectasia.
Bioscience reports39(5)(2019 05)
Zhang D,Zhou F,Zhao X,Liu B,Chen J,Yang J

Essential role of prostaglandin E
Scientific reports9(1)7650(2019 May)
Iwasaki R,Tsuge K,Kishimoto K,Hayashi Y,Iwaana T,Hohjoh H,Inazumi T,Kawahara A,Tsuchiya S,Sugimoto Y

A 3D Atlas of Hematopoietic Stem and Progenitor Cell Expansion by Multi-dimensional RNA-Seq Analysis.
Cell reports27(5)1567-1578.e5(2019 04)
Xue Y,Liu D,Cui G,Ding Y,Ai D,Gao S,Zhang Y,Suo S,Wang X,Lv P,Zhou C,Li Y,Chen X,Peng G,Jing N,Han JJ,Liu F

Atypical Chemokine Receptor 3 Generates Guidance Cues for CXCL12-Mediated Endothelial Cell Migration.
Frontiers in immunology101092(2019)
Tobia C,Chiodelli P,Barbieri A,Buraschi S,Ferrari E,Mitola S,Borsani G,Guerra J,Presta M

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FLT4 cDNA ORF clone, Danio rerio(Zebrafish)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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