lrfn4a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	lrfn4a
Entrez Gene ID	101882983
Full Name	leucine rich repeat and fibronectin type III domain containing 4a
Synonyms	si:ch211-250a23.3
General protein information	Preferred Names leucine rich repeat and fibronectin type III domain containing 4a Names leucine-rich repeat and fibronectin type-III domain-containing protein 4-like
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005161363.3	XP_005161420.1	leucine-rich repeat and fibronectin type-III domain-containing protein 4-like
XM_005161363.4	XP_005161420.1	leucine-rich repeat and fibronectin type-III domain-containing protein 4-like

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	LRFN4	XP_001107879.1
Canis lupus familiaris (dog)	LRFN4	XP_852214.1
Xenopus tropicalis (tropical clawed frog)	lrfn2	XP_004913581.1
Bos taurus (cattle)	LRFN4	NP_001193183.1
Danio rerio (zebrafish)	lrfn4b	XP_005166600.1
Homo sapiens (human)	LRFN4	NP_076941.2
Mus musculus (house mouse)	Lrfn4	NP_700437.2
Rattus norvegicus (Norway rat)	Lrfn4	NP_001102978.1
Danio rerio (zebrafish)	LOC101882983	XP_005161420.1

Gene Ontology

The following lrfn4a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lrfn4a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa25984	XM_005161363.4 Latest version!	Danio rerio leucine rich repeat and fibronectin type III domain containing 4a (lrfn4a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
ODa25984	XM_005161363.3	Danio rerio leucine rich repeat and fibronectin type III domain containing 4a (lrfn4a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lrfn4a ( XM_005161363.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161420.1 Danio rerio leucine rich repeat and fibronectin type III domain containing 4a (lrfn4a), mRNA.

Price & Availability↑

CloneID	ODa25984
Clone ID Related Accession (Same CDS sequence)	XM_005161363.3 , XM_005161363.4
Accession Version	XM_005161363.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2679bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	leucine-rich repeat and fibronectin type-III domain-containing protein 4-like
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005161363.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTACGCA CAGAACCAGG AAGTCCGTCT AGAGGAATCT ATCTGTTGCA AGAACATGAC 
AACCAGAAAT CGGACAAGTC AAGAAGATGT CCTGCTTTAT CAATCCCTGT CAGCACAATA 
CGTGTTTGCC CATGCCCCAT GTCACAATCA ACAATATTCT GGCTGGGTCT CCTAGGCTCT 
GTACTGCTGC AGAGTGGACA AGGTGTCATG GCAGGGGAGA CGTGGGGAAT GGTGTCCATC 
TGTCCTTTTC ACTGCGTGTG TCGGAACTTA TCAGAGTCTC TGAGCACTCT TTGTGTGAAT 
AAAGGTCTTC TCTTTGTACC ACCCAACATT GATCGCCGAA CTGTGGAGCT CCGTCTTGCT 
GACAACTACA TCCTTGAAGT GGGAGGTGCT GACTTTGCCA ATATGACAGG ACTAGTTGAC 
CTGACGTTGT CACGGAATAC CATCCATGCT CTTAAACCGC TGGCCTTCGC CGATCTAGAG 
AGTCTGCGTT CACTTCACCT TGACACCAAT CGTCTCACAG TAGTGGGACC TCAAGATCTG 
ACAGGTCTTG TTAACCTTCA GCATCTCATC ATAAACAACA ACCAGCTCAC AGATGTTTCA 
GCAGATGCTT TTGATGACTT CTTACTGACT CTGGAGGATC TGGACCTGTC CTACAACAAC 
CTTCGCAGAG TTCCTTGGGA ATCTATTCAG AACATGGCAA GCCTGCACAC ATTAAATCTA 
GATCATAATT TAATAGACCA GATTGCGGAG GGTTCTTTTG GTGAGCTCTA CAAACTGTCT 
CGGTTGGATA TGACTTCAAA CAGATTGCAG ACTCTTCCCC CTGATCCTCT GTTTTCCAGA 
TCCCAGATTG GGGTTGTGAG CCCTACACCA TACAATTCCA TTACCAGCCT TAACTTTGGT 
GGAAATCCCC TGCATTGCAA CTGTGAGCTG CTGTGGCTTC GACGTTTAAT TCGTGAGGAC 
GACATGGAGA CATGTGCCAC ACCCGCTCAT CTGGCAGGGC GCTATTTCTG GTCCATCCCA 
GAGGAAGAGT TCACGTGTGA GCCTCCACTG ATCACTCGTA ATACTAACAA GCTGTGGGTG 
TTGGAGGGCC AGAGAGCCAC ACTTAAATGT AGAGCCATTG GTGACCCTGA GCCAGTCATT 
CACTGGGTGT CACCAGATGA TCGTATTGTG GCCAACTCCA GTCGCATTCG CTCATACTAC 
AATGGTACAC TGGATTTTTT GGTGACCCGT GCCAGAGATG ACGGCACTTA CACTTGCATT 
GCAATTAATG CTGCAGGAGA ATCTACAGCT CTGGTAGATT TAAAGATCAT CCCTCTTCCC 
CACAGAGGGA ATGGTACAAT ATCCCTTATA AATCACAGAG ACCCTGGATC CTCTGATATC 
AGCAGTGGAC GAGGAGGTGT TGAAGGGACA GGAAACGGTG TTGTGACTGT ACGGAATGTC 
ACAGGAGGCA AAACAGATAC TGGAGAAAGA GGAAGTGGGA GCAGTAATGG AGGAACAGAA 
ACCCTGGTAG GAGCTCTAGG CGTGACATCC AACTCCGCTC AGATACGCTG GAAAATGGGT 
CGATCTTCAA TGCCTTTTTT GGTGTGGATG TATCAGATCC AGTACAATAG CACAGCAGAC 
GACGCACTTG TTTACAGAAT TCTGCCTCCA ACCAGTAATA GCTTCCTGCT GAAGAACTTG 
GTATCAGGAG CAGACTACAG CCTGTGTGTC TTGGCCATTT TCGATGATGG CATCTCCTCC 
TTGGCTACTA CAAAGGTGCT GGGTTGCATC CAGTTCAGCA CTAAAGAGGA TTATCCAGAA 
TGCCGTTCTC TGCACGCTCA CTTCCTGGGC GGCACACTGA CAGTGATGGT GGGAGGCGTG 
GTGGTAGTAA CCCTTCTGGT GTTCACCGTG GCTATGATGG TGCGCCATCG TGTCTGCGGA 
GAGTGTCGGG AAGATGCCAA CAGGCCTGGC AAGTTCCTTT CAACCAAAGG AGTGGATGTT 
TATGCCCAGA CGAACGGAAA CAACAGTATG ATGATGGTGG CATTGCCAAA CGGACTCTTG 
GCCCAAAGAA CAAAAGACAA ACAAAAGCCT AGCTCCCTCC CAAAACCTAA GCAAAGCCCT 
GAACCGCACC GTGGCACACG AGATAGGGGC GACGGGGCAG TGAGGGAGAA ATGCTTCAGT 
CCTCTCACAC CAGAAAGTGA GAGATTAACA CTGTACTATT CCCCAAGTAA CACACTACCC 
ACGCCAACGC AAAAGCGAGC AGGTAGACTC AAGTTAAGGA AGTCTCTGGA GAAAGACAGA 
GATGTAGACA GTCAAGTCTT GGCCTCACTT GCACTGCCAC AAGATGGAGA TGACGCAAAG 
GAAGGAGGCT CAGATTTGAG GCAGGCACTC AAAACCAAGC GTAGCTGCTC TTTTGATGTT 
GGGGAAATCA CCACCACCAC CTGTTACAGC TATGCCAAGC GGCTGAGCGT CATCTGGACA 
CGTCGTAGCC AGTCACTGCA TGGCATGCTT GTCCACTGTG CCTCCACCAC CAGCACATCC 
AGCACGGGTA GCAGCGAGCA GTACGGCCAC GGCCTAACAC AGAACTATCT ACATGCCTTC 
GCTTCCAACA ACTCCTCCAA TTCCAACTCA AACTCGACTT CAAACTCAAA CAGTAGCATG 
ATGGTCAATC CAAGAGACCT GGAGGAAAGT GTGGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161420.1
CDS	971..3649
Translation	MLRTEPGSPSRGIYLLQEHDNQKSDKSRRCPALSIPVSTIRVCPCPMSQSTIFWLGLLGSVLLQSGQGVMAGETWGMVSICPFHCVCRNLSESLSTLCVNKGLLFVPPNIDRRTVELRLADNYILEVGGADFANMTGLVDLTLSRNTIHALKPLAFADLESLRSLHLDTNRLTVVGPQDLTGLVNLQHLIINNNQLTDVSADAFDDFLLTLEDLDLSYNNLRRVPWESIQNMASLHTLNLDHNLIDQIAEGSFGELYKLSRLDMTSNRLQTLPPDPLFSRSQIGVVSPTPYNSITSLNFGGNPLHCNCELLWLRRLIREDDMETCATPAHLAGRYFWSIPEEEFTCEPPLITRNTNKLWVLEGQRATLKCRAIGDPEPVIHWVSPDDRIVANSSRIRSYYNGTLDFLVTRARDDGTYTCIAINAAGESTALVDLKIIPLPHRGNGTISLINHRDPGSSDISSGRGGVEGTGNGVVTVRNVTGGKTDTGERGSGSSNGGTETLVGALGVTSNSAQIRWKMGRSSMPFLVWMYQIQYNSTADDALVYRILPPTSNSFLLKNLVSGADYSLCVLAIFDDGISSLATTKVLGCIQFSTKEDYPECRSLHAHFLGGTLTVMVGGVVVVTLLVFTVAMMVRHRVCGECREDANRPGKFLSTKGVDVYAQTNGNNSMMMVALPNGLLAQRTKDKQKPSSLPKPKQSPEPHRGTRDRGDGAVREKCFSPLTPESERLTLYYSPSNTLPTPTQKRAGRLKLRKSLEKDRDVDSQVLASLALPQDGDDAKEGGSDLRQALKTKRSCSFDVGEITTTTCYSYAKRLSVIWTRRSQSLHGMLVHCASTTSTSSTGSSEQYGHGLTQNYLHAFASNNSSNSNSNSTSNSNSSMMVNPRDLEESVV

Target ORF information:

RefSeq Version	XM_005161363.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio leucine rich repeat and fibronectin type III domain containing 4a (lrfn4a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161363.3

ORF Insert Sequence:

ATGCTACGCA CAGAACCAGG AAGTCCGTCT AGAGGAATCT ATCTGTTGCA AGAACATGAC 
AACCAGAAAT CGGACAAGTC AAGAAGATGT CCTGCTTTAT CAATCCCTGT CAGCACAATA 
CGTGTTTGCC CATGCCCCAT GTCACAATCA ACAATATTCT GGCTGGGTCT CCTAGGCTCT 
GTACTGCTGC AGAGTGGACA AGGTGTCATG GCAGGGGAGA CGTGGGGAAT GGTGTCCATC 
TGTCCTTTTC ACTGCGTGTG TCGGAACTTA TCAGAGTCTC TGAGCACTCT TTGTGTGAAT 
AAAGGTCTTC TCTTTGTACC ACCCAACATT GATCGCCGAA CTGTGGAGCT CCGTCTTGCT 
GACAACTACA TCCTTGAAGT GGGAGGTGCT GACTTTGCCA ATATGACAGG ACTAGTTGAC 
CTGACGTTGT CACGGAATAC CATCCATGCT CTTAAACCGC TGGCCTTCGC CGATCTAGAG 
AGTCTGCGTT CACTTCACCT TGACACCAAT CGTCTCACAG TAGTGGGACC TCAAGATCTG 
ACAGGTCTTG TTAACCTTCA GCATCTCATC ATAAACAACA ACCAGCTCAC AGATGTTTCA 
GCAGATGCTT TTGATGACTT CTTACTGACT CTGGAGGATC TGGACCTGTC CTACAACAAC 
CTTCGCAGAG TTCCTTGGGA ATCTATTCAG AACATGGCAA GCCTGCACAC ATTAAATCTA 
GATCATAATT TAATAGACCA GATTGCGGAG GGTTCTTTTG GTGAGCTCTA CAAACTGTCT 
CGGTTGGATA TGACTTCAAA CAGATTGCAG ACTCTTCCCC CTGATCCTCT GTTTTCCAGA 
TCCCAGATTG GGGTTGTGAG CCCTACACCA TACAATTCCA TTACCAGCCT TAACTTTGGT 
GGAAATCCCC TGCATTGCAA CTGTGAGCTG CTGTGGCTTC GACGTTTAAT TCGTGAGGAC 
GACATGGAGA CATGTGCCAC ACCCGCTCAT CTGGCAGGGC GCTATTTCTG GTCCATCCCA 
GAGGAAGAGT TCACGTGTGA GCCTCCACTG ATCACTCGTA ATACTAACAA GCTGTGGGTG 
TTGGAGGGCC AGAGAGCCAC ACTTAAATGT AGAGCCATTG GTGACCCTGA GCCAGTCATT 
CACTGGGTGT CACCAGATGA TCGTATTGTG GCCAACTCCA GTCGCATTCG CTCATACTAC 
AATGGTACAC TGGATTTTTT GGTGACCCGT GCCAGAGATG ACGGCACTTA CACTTGCATT 
GCAATTAATG CTGCAGGAGA ATCTACAGCT CTGGTAGATT TAAAGATCAT CCCTCTTCCC 
CACAGAGGGA ATGGTACAAT ATCCCTTATA AATCACAGAG ACCCTGGATC CTCTGATATC 
AGCAGTGGAC GAGGAGGTGT TGAAGGGACA GGAAACGGTG TTGTGACTGT ACGGAATGTC 
ACAGGAGGCA AAACAGATAC TGGAGAAAGA GGAAGTGGGA GCAGTAATGG AGGAACAGAA 
ACCCTGGTAG GAGCTCTAGG CGTGACATCC AACTCCGCTC AGATACGCTG GAAAATGGGT 
CGATCTTCAA TGCCTTTTTT GGTGTGGATG TATCAGATCC AGTACAATAG CACAGCAGAC 
GACGCACTTG TTTACAGAAT TCTGCCTCCA ACCAGTAATA GCTTCCTGCT GAAGAACTTG 
GTATCAGGAG CAGACTACAG CCTGTGTGTC TTGGCCATTT TCGATGATGG CATCTCCTCC 
TTGGCTACTA CAAAGGTGCT GGGTTGCATC CAGTTCAGCA CTAAAGAGGA TTATCCAGAA 
TGCCGTTCTC TGCACGCTCA CTTCCTGGGC GGCACACTGA CAGTGATGGT GGGAGGCGTG 
GTGGTAGTAA CCCTTCTGGT GTTCACCGTG GCTATGATGG TGCGCCATCG TGTCTGCGGA 
GAGTGTCGGG AAGATGCCAA CAGGCCTGGC AAGTTCCTTT CAACCAAAGG AGTGGATGTT 
TATGCCCAGA CGAACGGAAA CAACAGTATG ATGATGGTGG CATTGCCAAA CGGACTCTTG 
GCCCAAAGAA CAAAAGACAA ACAAAAGCCT AGCTCCCTCC CAAAACCTAA GCAAAGCCCT 
GAACCGCACC GTGGCACACG AGATAGGGGC GACGGGGCAG TGAGGGAGAA ATGCTTCAGT 
CCTCTCACAC CAGAAAGTGA GAGATTAACA CTGTACTATT CCCCAAGTAA CACACTACCC 
ACGCCAACGC AAAAGCGAGC AGGTAGACTC AAGTTAAGGA AGTCTCTGGA GAAAGACAGA 
GATGTAGACA GTCAAGTCTT GGCCTCACTT GCACTGCCAC AAGATGGAGA TGACGCAAAG 
GAAGGAGGCT CAGATTTGAG GCAGGCACTC AAAACCAAGC GTAGCTGCTC TTTTGATGTT 
GGGGAAATCA CCACCACCAC CTGTTACAGC TATGCCAAGC GGCTGAGCGT CATCTGGACA 
CGTCGTAGCC AGTCACTGCA TGGCATGCTT GTCCACTGTG CCTCCACCAC CAGCACATCC 
AGCACGGGTA GCAGCGAGCA GTACGGCCAC GGCCTAACAC AGAACTATCT ACATGCCTTC 
GCTTCCAACA ACTCCTCCAA TTCCAACTCA AACTCGACTT CAAACTCAAA CAGTAGCATG 
ATGGTCAATC CAAGAGACCT GGAGGAAAGT GTGGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lrfn4a ( XM_005161363.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161420.1 Danio rerio leucine rich repeat and fibronectin type III domain containing 4a (lrfn4a), mRNA.

Price & Availability↑

CloneID	ODa25984
Clone ID Related Accession (Same CDS sequence)	XM_005161363.3 , XM_005161363.4
Accession Version	XM_005161363.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2679bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	leucine-rich repeat and fibronectin type-III domain-containing protein 4-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005161363.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTACGCA CAGAACCAGG AAGTCCGTCT AGAGGAATCT ATCTGTTGCA AGAACATGAC 
AACCAGAAAT CGGACAAGTC AAGAAGATGT CCTGCTTTAT CAATCCCTGT CAGCACAATA 
CGTGTTTGCC CATGCCCCAT GTCACAATCA ACAATATTCT GGCTGGGTCT CCTAGGCTCT 
GTACTGCTGC AGAGTGGACA AGGTGTCATG GCAGGGGAGA CGTGGGGAAT GGTGTCCATC 
TGTCCTTTTC ACTGCGTGTG TCGGAACTTA TCAGAGTCTC TGAGCACTCT TTGTGTGAAT 
AAAGGTCTTC TCTTTGTACC ACCCAACATT GATCGCCGAA CTGTGGAGCT CCGTCTTGCT 
GACAACTACA TCCTTGAAGT GGGAGGTGCT GACTTTGCCA ATATGACAGG ACTAGTTGAC 
CTGACGTTGT CACGGAATAC CATCCATGCT CTTAAACCGC TGGCCTTCGC CGATCTAGAG 
AGTCTGCGTT CACTTCACCT TGACACCAAT CGTCTCACAG TAGTGGGACC TCAAGATCTG 
ACAGGTCTTG TTAACCTTCA GCATCTCATC ATAAACAACA ACCAGCTCAC AGATGTTTCA 
GCAGATGCTT TTGATGACTT CTTACTGACT CTGGAGGATC TGGACCTGTC CTACAACAAC 
CTTCGCAGAG TTCCTTGGGA ATCTATTCAG AACATGGCAA GCCTGCACAC ATTAAATCTA 
GATCATAATT TAATAGACCA GATTGCGGAG GGTTCTTTTG GTGAGCTCTA CAAACTGTCT 
CGGTTGGATA TGACTTCAAA CAGATTGCAG ACTCTTCCCC CTGATCCTCT GTTTTCCAGA 
TCCCAGATTG GGGTTGTGAG CCCTACACCA TACAATTCCA TTACCAGCCT TAACTTTGGT 
GGAAATCCCC TGCATTGCAA CTGTGAGCTG CTGTGGCTTC GACGTTTAAT TCGTGAGGAC 
GACATGGAGA CATGTGCCAC ACCCGCTCAT CTGGCAGGGC GCTATTTCTG GTCCATCCCA 
GAGGAAGAGT TCACGTGTGA GCCTCCACTG ATCACTCGTA ATACTAACAA GCTGTGGGTG 
TTGGAGGGCC AGAGAGCCAC ACTTAAATGT AGAGCCATTG GTGACCCTGA GCCAGTCATT 
CACTGGGTGT CACCAGATGA TCGTATTGTG GCCAACTCCA GTCGCATTCG CTCATACTAC 
AATGGTACAC TGGATTTTTT GGTGACCCGT GCCAGAGATG ACGGCACTTA CACTTGCATT 
GCAATTAATG CTGCAGGAGA ATCTACAGCT CTGGTAGATT TAAAGATCAT CCCTCTTCCC 
CACAGAGGGA ATGGTACAAT ATCCCTTATA AATCACAGAG ACCCTGGATC CTCTGATATC 
AGCAGTGGAC GAGGAGGTGT TGAAGGGACA GGAAACGGTG TTGTGACTGT ACGGAATGTC 
ACAGGAGGCA AAACAGATAC TGGAGAAAGA GGAAGTGGGA GCAGTAATGG AGGAACAGAA 
ACCCTGGTAG GAGCTCTAGG CGTGACATCC AACTCCGCTC AGATACGCTG GAAAATGGGT 
CGATCTTCAA TGCCTTTTTT GGTGTGGATG TATCAGATCC AGTACAATAG CACAGCAGAC 
GACGCACTTG TTTACAGAAT TCTGCCTCCA ACCAGTAATA GCTTCCTGCT GAAGAACTTG 
GTATCAGGAG CAGACTACAG CCTGTGTGTC TTGGCCATTT TCGATGATGG CATCTCCTCC 
TTGGCTACTA CAAAGGTGCT GGGTTGCATC CAGTTCAGCA CTAAAGAGGA TTATCCAGAA 
TGCCGTTCTC TGCACGCTCA CTTCCTGGGC GGCACACTGA CAGTGATGGT GGGAGGCGTG 
GTGGTAGTAA CCCTTCTGGT GTTCACCGTG GCTATGATGG TGCGCCATCG TGTCTGCGGA 
GAGTGTCGGG AAGATGCCAA CAGGCCTGGC AAGTTCCTTT CAACCAAAGG AGTGGATGTT 
TATGCCCAGA CGAACGGAAA CAACAGTATG ATGATGGTGG CATTGCCAAA CGGACTCTTG 
GCCCAAAGAA CAAAAGACAA ACAAAAGCCT AGCTCCCTCC CAAAACCTAA GCAAAGCCCT 
GAACCGCACC GTGGCACACG AGATAGGGGC GACGGGGCAG TGAGGGAGAA ATGCTTCAGT 
CCTCTCACAC CAGAAAGTGA GAGATTAACA CTGTACTATT CCCCAAGTAA CACACTACCC 
ACGCCAACGC AAAAGCGAGC AGGTAGACTC AAGTTAAGGA AGTCTCTGGA GAAAGACAGA 
GATGTAGACA GTCAAGTCTT GGCCTCACTT GCACTGCCAC AAGATGGAGA TGACGCAAAG 
GAAGGAGGCT CAGATTTGAG GCAGGCACTC AAAACCAAGC GTAGCTGCTC TTTTGATGTT 
GGGGAAATCA CCACCACCAC CTGTTACAGC TATGCCAAGC GGCTGAGCGT CATCTGGACA 
CGTCGTAGCC AGTCACTGCA TGGCATGCTT GTCCACTGTG CCTCCACCAC CAGCACATCC 
AGCACGGGTA GCAGCGAGCA GTACGGCCAC GGCCTAACAC AGAACTATCT ACATGCCTTC 
GCTTCCAACA ACTCCTCCAA TTCCAACTCA AACTCGACTT CAAACTCAAA CAGTAGCATG 
ATGGTCAATC CAAGAGACCT GGAGGAAAGT GTGGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161420.1
CDS	945..3623
Translation	MLRTEPGSPSRGIYLLQEHDNQKSDKSRRCPALSIPVSTIRVCPCPMSQSTIFWLGLLGSVLLQSGQGVMAGETWGMVSICPFHCVCRNLSESLSTLCVNKGLLFVPPNIDRRTVELRLADNYILEVGGADFANMTGLVDLTLSRNTIHALKPLAFADLESLRSLHLDTNRLTVVGPQDLTGLVNLQHLIINNNQLTDVSADAFDDFLLTLEDLDLSYNNLRRVPWESIQNMASLHTLNLDHNLIDQIAEGSFGELYKLSRLDMTSNRLQTLPPDPLFSRSQIGVVSPTPYNSITSLNFGGNPLHCNCELLWLRRLIREDDMETCATPAHLAGRYFWSIPEEEFTCEPPLITRNTNKLWVLEGQRATLKCRAIGDPEPVIHWVSPDDRIVANSSRIRSYYNGTLDFLVTRARDDGTYTCIAINAAGESTALVDLKIIPLPHRGNGTISLINHRDPGSSDISSGRGGVEGTGNGVVTVRNVTGGKTDTGERGSGSSNGGTETLVGALGVTSNSAQIRWKMGRSSMPFLVWMYQIQYNSTADDALVYRILPPTSNSFLLKNLVSGADYSLCVLAIFDDGISSLATTKVLGCIQFSTKEDYPECRSLHAHFLGGTLTVMVGGVVVVTLLVFTVAMMVRHRVCGECREDANRPGKFLSTKGVDVYAQTNGNNSMMMVALPNGLLAQRTKDKQKPSSLPKPKQSPEPHRGTRDRGDGAVREKCFSPLTPESERLTLYYSPSNTLPTPTQKRAGRLKLRKSLEKDRDVDSQVLASLALPQDGDDAKEGGSDLRQALKTKRSCSFDVGEITTTTCYSYAKRLSVIWTRRSQSLHGMLVHCASTTSTSSTGSSEQYGHGLTQNYLHAFASNNSSNSNSNSTSNSNSSMMVNPRDLEESVV

Target ORF information:

RefSeq Version	XM_005161363.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio leucine rich repeat and fibronectin type III domain containing 4a (lrfn4a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161363.4

ORF Insert Sequence:

ATGCTACGCA CAGAACCAGG AAGTCCGTCT AGAGGAATCT ATCTGTTGCA AGAACATGAC 
AACCAGAAAT CGGACAAGTC AAGAAGATGT CCTGCTTTAT CAATCCCTGT CAGCACAATA 
CGTGTTTGCC CATGCCCCAT GTCACAATCA ACAATATTCT GGCTGGGTCT CCTAGGCTCT 
GTACTGCTGC AGAGTGGACA AGGTGTCATG GCAGGGGAGA CGTGGGGAAT GGTGTCCATC 
TGTCCTTTTC ACTGCGTGTG TCGGAACTTA TCAGAGTCTC TGAGCACTCT TTGTGTGAAT 
AAAGGTCTTC TCTTTGTACC ACCCAACATT GATCGCCGAA CTGTGGAGCT CCGTCTTGCT 
GACAACTACA TCCTTGAAGT GGGAGGTGCT GACTTTGCCA ATATGACAGG ACTAGTTGAC 
CTGACGTTGT CACGGAATAC CATCCATGCT CTTAAACCGC TGGCCTTCGC CGATCTAGAG 
AGTCTGCGTT CACTTCACCT TGACACCAAT CGTCTCACAG TAGTGGGACC TCAAGATCTG 
ACAGGTCTTG TTAACCTTCA GCATCTCATC ATAAACAACA ACCAGCTCAC AGATGTTTCA 
GCAGATGCTT TTGATGACTT CTTACTGACT CTGGAGGATC TGGACCTGTC CTACAACAAC 
CTTCGCAGAG TTCCTTGGGA ATCTATTCAG AACATGGCAA GCCTGCACAC ATTAAATCTA 
GATCATAATT TAATAGACCA GATTGCGGAG GGTTCTTTTG GTGAGCTCTA CAAACTGTCT 
CGGTTGGATA TGACTTCAAA CAGATTGCAG ACTCTTCCCC CTGATCCTCT GTTTTCCAGA 
TCCCAGATTG GGGTTGTGAG CCCTACACCA TACAATTCCA TTACCAGCCT TAACTTTGGT 
GGAAATCCCC TGCATTGCAA CTGTGAGCTG CTGTGGCTTC GACGTTTAAT TCGTGAGGAC 
GACATGGAGA CATGTGCCAC ACCCGCTCAT CTGGCAGGGC GCTATTTCTG GTCCATCCCA 
GAGGAAGAGT TCACGTGTGA GCCTCCACTG ATCACTCGTA ATACTAACAA GCTGTGGGTG 
TTGGAGGGCC AGAGAGCCAC ACTTAAATGT AGAGCCATTG GTGACCCTGA GCCAGTCATT 
CACTGGGTGT CACCAGATGA TCGTATTGTG GCCAACTCCA GTCGCATTCG CTCATACTAC 
AATGGTACAC TGGATTTTTT GGTGACCCGT GCCAGAGATG ACGGCACTTA CACTTGCATT 
GCAATTAATG CTGCAGGAGA ATCTACAGCT CTGGTAGATT TAAAGATCAT CCCTCTTCCC 
CACAGAGGGA ATGGTACAAT ATCCCTTATA AATCACAGAG ACCCTGGATC CTCTGATATC 
AGCAGTGGAC GAGGAGGTGT TGAAGGGACA GGAAACGGTG TTGTGACTGT ACGGAATGTC 
ACAGGAGGCA AAACAGATAC TGGAGAAAGA GGAAGTGGGA GCAGTAATGG AGGAACAGAA 
ACCCTGGTAG GAGCTCTAGG CGTGACATCC AACTCCGCTC AGATACGCTG GAAAATGGGT 
CGATCTTCAA TGCCTTTTTT GGTGTGGATG TATCAGATCC AGTACAATAG CACAGCAGAC 
GACGCACTTG TTTACAGAAT TCTGCCTCCA ACCAGTAATA GCTTCCTGCT GAAGAACTTG 
GTATCAGGAG CAGACTACAG CCTGTGTGTC TTGGCCATTT TCGATGATGG CATCTCCTCC 
TTGGCTACTA CAAAGGTGCT GGGTTGCATC CAGTTCAGCA CTAAAGAGGA TTATCCAGAA 
TGCCGTTCTC TGCACGCTCA CTTCCTGGGC GGCACACTGA CAGTGATGGT GGGAGGCGTG 
GTGGTAGTAA CCCTTCTGGT GTTCACCGTG GCTATGATGG TGCGCCATCG TGTCTGCGGA 
GAGTGTCGGG AAGATGCCAA CAGGCCTGGC AAGTTCCTTT CAACCAAAGG AGTGGATGTT 
TATGCCCAGA CGAACGGAAA CAACAGTATG ATGATGGTGG CATTGCCAAA CGGACTCTTG 
GCCCAAAGAA CAAAAGACAA ACAAAAGCCT AGCTCCCTCC CAAAACCTAA GCAAAGCCCT 
GAACCGCACC GTGGCACACG AGATAGGGGC GACGGGGCAG TGAGGGAGAA ATGCTTCAGT 
CCTCTCACAC CAGAAAGTGA GAGATTAACA CTGTACTATT CCCCAAGTAA CACACTACCC 
ACGCCAACGC AAAAGCGAGC AGGTAGACTC AAGTTAAGGA AGTCTCTGGA GAAAGACAGA 
GATGTAGACA GTCAAGTCTT GGCCTCACTT GCACTGCCAC AAGATGGAGA TGACGCAAAG 
GAAGGAGGCT CAGATTTGAG GCAGGCACTC AAAACCAAGC GTAGCTGCTC TTTTGATGTT 
GGGGAAATCA CCACCACCAC CTGTTACAGC TATGCCAAGC GGCTGAGCGT CATCTGGACA 
CGTCGTAGCC AGTCACTGCA TGGCATGCTT GTCCACTGTG CCTCCACCAC CAGCACATCC 
AGCACGGGTA GCAGCGAGCA GTACGGCCAC GGCCTAACAC AGAACTATCT ACATGCCTTC 
GCTTCCAACA ACTCCTCCAA TTCCAACTCA AACTCGACTT CAAACTCAAA CAGTAGCATG 
ATGGTCAATC CAAGAGACCT GGAGGAAAGT GTGGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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lrfn4a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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