THEMIS2 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	THEMIS2
Entrez Gene ID	100535600
Full Name	thymocyte selection associated family member 2
Synonyms	si:ch1073-55d10.2
General protein information	Preferred Names thymocyte selection associated family member 2 Names protein THEMIS2
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021466810.1	XP_021322485.1	protein THEMIS2 isoform X1
XM_005157857.4	XP_005157914.1	protein THEMIS2 isoform X3
XM_009292033.2	XP_009290308.1	protein THEMIS2 isoform X2
XM_021466811.1	XP_021322486.1	protein THEMIS2 isoform X2
XM_005157857.3	XP_005157914.1	protein THEMIS2 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Themis2	NP_001028480.1
Canis lupus familiaris (dog)	THEMIS2	XP_003638987.1
Danio rerio (zebrafish)	LOC100535600	XP_005157914.1
Macaca mulatta (Rhesus monkey)	THEMIS2	XP_001112611.1
Bos taurus (cattle)	THEMIS2	XP_002685734.1
Homo sapiens (human)	THEMIS2	NP_001099026.1
Xenopus tropicalis (tropical clawed frog)	themis2	XP_004912375.1
Pan troglodytes (chimpanzee)	THEMIS2	XP_003307966.1
Rattus norvegicus (Norway rat)	Themis2	NP_001258409.1
Gallus gallus (chicken)	C23H1ORF38	XP_417712.2

Gene Ontology

The following THEMIS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the THEMIS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa149315	XM_021466810.1 Latest version!	Danio rerio thymocyte selection associated family member 2 (themis2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa149316	XM_021466811.1 Latest version!	Danio rerio thymocyte selection associated family member 2 (themis2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa21048	XM_005157857.4 Latest version!	Danio rerio thymocyte selection associated family member 2 (themis2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa50202	XM_009292033.2 Latest version!	Danio rerio thymocyte selection associated family member 2 (themis2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
ODa21048	XM_005157857.3	Danio rerio thymocyte selection associated family member 2 (themis2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

THEMIS2 ( XM_021466810.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021322485.1 Danio rerio thymocyte selection associated family member 2 (themis2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa149315
Clone ID Related Accession (Same CDS sequence)	XM_021466810.1
Accession Version	XM_021466810.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	protein THEMIS2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGACC TCCAGTCCCT GCAAGCCTTC ATCAACTGTC TGGATCAGCG CTCTCTGCCC 
CGCATCCTTC AGGTCTGCTC GGGGGTTTAT TTTCAAGGGT CTGTGTATGA GATTTCGGGG 
AGTGAAGTGT GTTTATCCAC TGGAGATTTG GTGAAGATCA TTGGTTTGGA GCTCCTGTCT 
GTGTCTTGTG AAGATGTCAA CACGGGAGAT TCATTTGTAT TGCCTATTCA CTACACAGGT 
AATTTCAGAC TCGTCGCTGA AGACCTACCT TATAACACAG TGGAAGAGTT GGTGGGTTTG 
TGTCCAGTCG GAGTGGACGC CTGCGGCTCC TTCACCTTCA GAAGCACACA AGAGCTGATA 
GTGGACAACT TCACGCTGGC CCCAGACGAA CATTTGTCCC TGCTTTCGGT GGAGGTCTCT 
GAGGATGGAG AAAGGCTGGC TCGGTGTCAG GTGTTAGGGC ATAACATGGC CTACGCCGAG 
CTTCTGCTTC CTCTGTCTCT GAAAGGAGAG TTTTACGAGT GTGAGGATGA GCGCGGATAC 
TTTCTGCAGG AGATCATGTC CTCGGCCAGA CTCAGCTGCC GCCGCTTCTG CAACACAAAC 
ACCAAGAGCA GTGGCGGATT GCTGATGTTC AGCCCGGTCT ATGAAATCAG CGCCATTATG 
CATCTAAGGA AGAACATAGT CAAGTTTCCC TCCACCCTAG AAGTAGATGT CCAGGACGTG 
ACGGACCAAT TCCAGGATTT AGTCTTCATA ACTCCACTAT CACTGCCTGA AGTCGTGAGC 
CAGCCCAAAG AGTCTTTCCC GACAATGGCA GAAATCCTGG AAGCTCCAGA AGGTAACCAG 
TACTTCAGCT GCAGCTGGTT TGGTGGACTA CAAAAAGGCA AGAGCCTCAT ACTCCACGAA 
TGTCACCAAA AAACAATGAT ATTAGCCTCG ACTCCAAAGG GGAAGAAGGG AAAACAGTAC 
TTCATGATCT CCGACGACTA TGGAGGTCGC ATGAGGAGAC GCGCTCGGGA GTTCGGCTCA 
GTCTACGAGT TATATTTGGC GTCTTCTCAA TCTCCAGGAC TCAAAGTAAG CGTGAGCCGG 
GACTATGAAG CCGATGAGGA CGAGGGAATG CCTGCGCTCG GTGTCGGAGA AAATCTAGAG 
GTTTTGGGGA TGAGTTTGAT GGATAGACTC AAACCTGGAG CTGCTGAGAA AGTAGAAAGT 
CTAATTTGTA AACGGACTCA TGAGGTGGAC GATGATGATG ATGATGATGA AGATGATAGT 
GAGGAAATCT CAATGCCGCT GTTTATGGCG GGACACTTTG TGGAGAAGCT TTCGGATAAT 
AAGAAGTATC GATTAAACGA TTTAGTGAGC GGCTCTTTAC CGGTTGATGT CAAAGTTGTG 
ACTCGGGATA AAGATCTGGA GAAGGATCCG CTGATGGGGT TACCAGCGTT AAAGCTGGAG 
GAGACATTCA CTGAGACCAC CGTGTTGACG AGTCTACCCC ATAATCCAGA TTGGTGCTTT 
GAGTTGCCGG TTCGTTGGTT GCAAATGTCA GTCTGTTTCA CGTCTGATGA TCTGCCGTGG 
TCTCGGCCTC CAGAGCTTCA CGTTGAAACG GTCACCGAGA TGACGGAGAA ATTCTATTAC 
GAATATCACA AACTCATTGG CAAAACTGAG GAGCCGCCTC CACGTCCACC AAAAAGGAAG 
AAGTCCAGTT GTGACGCAAA CAAAAAGGTT CAATCTTTAC CGAGCTTGGG GAGTTCAGTG 
AGCAAACAAC TGGACAAACT TTCGTTGGGT CCGAAAGTAG AAAAACGCTC CGTTCCTCCA 
CCTCCGCAAG ACGAGCAGAC CCTACATCAG CCTCCTCCGC TGTTGCCTAG AAAGAGCATA 
TCGCTAGCAG AAAGTTCCAG TATGCCCAAC ATGTATGTGA AAAGCCCTAG AATGTCTCAC 
AAATCAGGTA CGTTTACGGA TTACATTCAG ATACTAGTGG TTCTAAATAA GATGTATACT 
TTAACCTTTT CTTATCTTCC TCTAATAAAA CAGCGAAGAG ACTTTCTGAC TCGGATCATG 
ACTACGAGTC TATCGAGGAC GCATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021322485.1
CDS	413..2482
Translation	MGDLQSLQAFINCLDQRSLPRILQVCSGVYFQGSVYEISGSEVCLSTGDLVKIIGLELLSVSCEDVNTGDSFVLPIHYTGNFRLVAEDLPYNTVEELVGLCPVGVDACGSFTFRSTQELIVDNFTLAPDEHLSLLSVEVSEDGERLARCQVLGHNMAYAELLLPLSLKGEFYECEDERGYFLQEIMSSARLSCRRFCNTNTKSSGGLLMFSPVYEISAIMHLRKNIVKFPSTLEVDVQDVTDQFQDLVFITPLSLPEVVSQPKESFPTMAEILEAPEGNQYFSCSWFGGLQKGKSLILHECHQKTMILASTPKGKKGKQYFMISDDYGGRMRRRAREFGSVYELYLASSQSPGLKVSVSRDYEADEDEGMPALGVGENLEVLGMSLMDRLKPGAAEKVESLICKRTHEVDDDDDDDEDDSEEISMPLFMAGHFVEKLSDNKKYRLNDLVSGSLPVDVKVVTRDKDLEKDPLMGLPALKLEETFTETTVLTSLPHNPDWCFELPVRWLQMSVCFTSDDLPWSRPPELHVETVTEMTEKFYYEYHKLIGKTEEPPPRPPKRKKSSCDANKKVQSLPSLGSSVSKQLDKLSLGPKVEKRSVPPPPQDEQTLHQPPPLLPRKSISLAESSSMPNMYVKSPRMSHKSGTFTDYIQILVVLNKMYTLTFSYLPLIKQRRDFLTRIMTTSLSRTHL

Target ORF information:

RefSeq Version	XM_021466810.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio thymocyte selection associated family member 2 (themis2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021466810.1

ORF Insert Sequence:

ATGGGAGACC TCCAGTCCCT GCAAGCCTTC ATCAACTGTC TGGATCAGCG CTCTCTGCCC 
CGCATCCTTC AGGTCTGCTC GGGGGTTTAT TTTCAAGGGT CTGTGTATGA GATTTCGGGG 
AGTGAAGTGT GTTTATCCAC TGGAGATTTG GTGAAGATCA TTGGTTTGGA GCTCCTGTCT 
GTGTCTTGTG AAGATGTCAA CACGGGAGAT TCATTTGTAT TGCCTATTCA CTACACAGGT 
AATTTCAGAC TCGTCGCTGA AGACCTACCT TATAACACAG TGGAAGAGTT GGTGGGTTTG 
TGTCCAGTCG GAGTGGACGC CTGCGGCTCC TTCACCTTCA GAAGCACACA AGAGCTGATA 
GTGGACAACT TCACGCTGGC CCCAGACGAA CATTTGTCCC TGCTTTCGGT GGAGGTCTCT 
GAGGATGGAG AAAGGCTGGC TCGGTGTCAG GTGTTAGGGC ATAACATGGC CTACGCCGAG 
CTTCTGCTTC CTCTGTCTCT GAAAGGAGAG TTTTACGAGT GTGAGGATGA GCGCGGATAC 
TTTCTGCAGG AGATCATGTC CTCGGCCAGA CTCAGCTGCC GCCGCTTCTG CAACACAAAC 
ACCAAGAGCA GTGGCGGATT GCTGATGTTC AGCCCGGTCT ATGAAATCAG CGCCATTATG 
CATCTAAGGA AGAACATAGT CAAGTTTCCC TCCACCCTAG AAGTAGATGT CCAGGACGTG 
ACGGACCAAT TCCAGGATTT AGTCTTCATA ACTCCACTAT CACTGCCTGA AGTCGTGAGC 
CAGCCCAAAG AGTCTTTCCC GACAATGGCA GAAATCCTGG AAGCTCCAGA AGGTAACCAG 
TACTTCAGCT GCAGCTGGTT TGGTGGACTA CAAAAAGGCA AGAGCCTCAT ACTCCACGAA 
TGTCACCAAA AAACAATGAT ATTAGCCTCG ACTCCAAAGG GGAAGAAGGG AAAACAGTAC 
TTCATGATCT CCGACGACTA TGGAGGTCGC ATGAGGAGAC GCGCTCGGGA GTTCGGCTCA 
GTCTACGAGT TATATTTGGC GTCTTCTCAA TCTCCAGGAC TCAAAGTAAG CGTGAGCCGG 
GACTATGAAG CCGATGAGGA CGAGGGAATG CCTGCGCTCG GTGTCGGAGA AAATCTAGAG 
GTTTTGGGGA TGAGTTTGAT GGATAGACTC AAACCTGGAG CTGCTGAGAA AGTAGAAAGT 
CTAATTTGTA AACGGACTCA TGAGGTGGAC GATGATGATG ATGATGATGA AGATGATAGT 
GAGGAAATCT CAATGCCGCT GTTTATGGCG GGACACTTTG TGGAGAAGCT TTCGGATAAT 
AAGAAGTATC GATTAAACGA TTTAGTGAGC GGCTCTTTAC CGGTTGATGT CAAAGTTGTG 
ACTCGGGATA AAGATCTGGA GAAGGATCCG CTGATGGGGT TACCAGCGTT AAAGCTGGAG 
GAGACATTCA CTGAGACCAC CGTGTTGACG AGTCTACCCC ATAATCCAGA TTGGTGCTTT 
GAGTTGCCGG TTCGTTGGTT GCAAATGTCA GTCTGTTTCA CGTCTGATGA TCTGCCGTGG 
TCTCGGCCTC CAGAGCTTCA CGTTGAAACG GTCACCGAGA TGACGGAGAA ATTCTATTAC 
GAATATCACA AACTCATTGG CAAAACTGAG GAGCCGCCTC CACGTCCACC AAAAAGGAAG 
AAGTCCAGTT GTGACGCAAA CAAAAAGGTT CAATCTTTAC CGAGCTTGGG GAGTTCAGTG 
AGCAAACAAC TGGACAAACT TTCGTTGGGT CCGAAAGTAG AAAAACGCTC CGTTCCTCCA 
CCTCCGCAAG ACGAGCAGAC CCTACATCAG CCTCCTCCGC TGTTGCCTAG AAAGAGCATA 
TCGCTAGCAG AAAGTTCCAG TATGCCCAAC ATGTATGTGA AAAGCCCTAG AATGTCTCAC 
AAATCAGGTA CGTTTACGGA TTACATTCAG ATACTAGTGG TTCTAAATAA GATGTATACT 
TTAACCTTTT CTTATCTTCC TCTAATAAAA CAGCGAAGAG ACTTTCTGAC TCGGATCATG 
ACTACGAGTC TATCGAGGAC GCATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THEMIS2 ( XM_021466811.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021322486.1 Danio rerio thymocyte selection associated family member 2 (themis2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa149316
Clone ID Related Accession (Same CDS sequence)	XM_021466811.1
Accession Version	XM_021466811.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	protein THEMIS2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGACC TCCAGTCCCT GCAAGCCTTC ATCAACTGTC TGGATCAGCG CTCTCTGCCC 
CGCATCCTTC AGGTCTGCTC GGGGGTTTAT TTTCAAGGGT CTGTGTATGA GATTTCGGGG 
AGTGAAGTGT GTTTATCCAC TGGAGATTTG GTGAAGATCA TTGGTTTGGA GCTCCTGTCT 
GTGTCTTGTG AAGATGTCAA CACGGGAGAT TCATTTGTAT TGCCTATTCA CTACACAGGT 
AATTTCAGAC TCGTCGCTGA AGACCTACCT TATAACACAG TGGAAGAGTT GGTGGGTTTG 
TGTCCAGTCG GAGTGGACGC CTGCGGCTCC TTCACCTTCA GAAGCACACA AGAGCTGATA 
GTGGACAACT TCACGCTGGC CCCAGACGAA CATTTGTCCC TGCTTTCGGT GGAGGTCTCT 
GAGGATGGAG AAAGGCTGGC TCGGTGTCAG GTGTTAGGGC ATAACATGGC CTACGCCGAG 
CTTCTGCTTC CTCTGTCTCT GAAAGGAGAG TTTTACGAGT GTGAGGATGA GCGCGGATAC 
TTTCTGCAGG AGATCATGTC CTCGGCCAGA CTCAGCTGCC GCCGCTTCTG CAACACAAAC 
ACCAAGAGCA GTGGCGGATT GCTGATGTTC AGCCCGGTCT ATGAAATCAG CGCCATTATG 
CATCTAAGGA AGAACATAGT CAAGTTTCCC TCCACCCTAG AAGTAGATGT CCAGGACGTG 
ACGGACCAAT TCCAGGATTT AGTCTTCATA ACTCCACTAT CACTGCCTGA AGTCGTGAGC 
CAGCCCAAAG AGTCTTTCCC GACAATGGCA GAAATCCTGG AAGCTCCAGA AGGTAACCAG 
TACTTCAGCT GCAGCTGGTT TGGTGGACTA CAAAAAGGCA AGAGCCTCAT ACTCCACGAA 
TGTCACCAAA AAACAATGAT ATTAGCCTCG ACTCCAAAGG GGAAGAAGGG AAAACAGTAC 
TTCATGATCT CCGACGACTA TGGAGGTCGC ATGAGGAGAC GCGCTCGGGA GTTCGGCTCA 
GTCTACGAGT TATATTTGGC GTCTTCTCAA TCTCCAGGAC TCAAAGTAAG CGTGAGCCGG 
GACTATGAAG CCGATGAGGA CGAGGGAATG CCTGCGCTCG GTGTCGGAGA AAATCTAGAG 
GTTTTGGGGA TGAGTTTGAT GGATAGACTC AAACCTGGAG CTGCTGAGAA AGTAGAAAGT 
CTAATTTGTA AACGGACTCA TGAGGTGGAC GATGATGATG ATGATGATGA AGATGATAGT 
GAGGAAATCT CAATGCCGCT GTTTATGGCG GGACACTTTG TGGAGAAGCT TTCGGATAAT 
AAGAAGTATC GATTAAACGA TTTAGTGAGC GGCTCTTTAC CGGTTGATGT CAAAGTTGTG 
ACTCGGGATA AAGATCTGGA GAAGGATCCG CTGATGGGGT TACCAGCGTT AAAGCTGGAG 
GAGACATTCA CTGAGACCAC CGTGTTGACG AGTCTACCCC ATAATCCAGA TTGGTGCTTT 
GAGTTGCCGG TTCGTTGGTT GCAAATGTCA GTCTGTTTCA CGTCTGATGA TCTGCCGTGG 
TCTCGGCCTC CAGAGCTTCA CGTTGAAACG GTCACCGAGA TGACGGAGAA ATTCTATTAC 
GAATATCACA AACTCATTGG CAAAACTGAG GAGCCGCCTC CACGTCCACC AAAAAGGAAG 
AAGTCCAGTT GTGACGCAAA CAAAAAGGTT CAATCTTTAC CGAGCTTGGG GAGTTCAGTG 
AGCAAACAAC TGGACAAACT TTCGTTGGGT CCGAAAGTAG AAAAACGCTC CGTTCCTCCA 
CCTCCGCAAG ACGAGACCCT ACATCAGCCT CCTCCGCTGT TGCCTAGAAA GAGCATATCG 
CTAGCAGAAA GTTCCAGTAT GCCCAACATG TATGTGAAAA GCCCTAGAAT GTCTCACAAA 
TCAGGTACGT TTACGGATTA CATTCAGATA CTAGTGGTTC TAAATAAGAT GTATACTTTA 
ACCTTTTCTT ATCTTCCTCT AATAAAACAG CGAAGAGACT TTCTGACTCG GATCATGACT 
ACGAGTCTAT CGAGGACGCA TTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021322486.1
CDS	421..2487
Translation	MGDLQSLQAFINCLDQRSLPRILQVCSGVYFQGSVYEISGSEVCLSTGDLVKIIGLELLSVSCEDVNTGDSFVLPIHYTGNFRLVAEDLPYNTVEELVGLCPVGVDACGSFTFRSTQELIVDNFTLAPDEHLSLLSVEVSEDGERLARCQVLGHNMAYAELLLPLSLKGEFYECEDERGYFLQEIMSSARLSCRRFCNTNTKSSGGLLMFSPVYEISAIMHLRKNIVKFPSTLEVDVQDVTDQFQDLVFITPLSLPEVVSQPKESFPTMAEILEAPEGNQYFSCSWFGGLQKGKSLILHECHQKTMILASTPKGKKGKQYFMISDDYGGRMRRRAREFGSVYELYLASSQSPGLKVSVSRDYEADEDEGMPALGVGENLEVLGMSLMDRLKPGAAEKVESLICKRTHEVDDDDDDDEDDSEEISMPLFMAGHFVEKLSDNKKYRLNDLVSGSLPVDVKVVTRDKDLEKDPLMGLPALKLEETFTETTVLTSLPHNPDWCFELPVRWLQMSVCFTSDDLPWSRPPELHVETVTEMTEKFYYEYHKLIGKTEEPPPRPPKRKKSSCDANKKVQSLPSLGSSVSKQLDKLSLGPKVEKRSVPPPPQDETLHQPPPLLPRKSISLAESSSMPNMYVKSPRMSHKSGTFTDYIQILVVLNKMYTLTFSYLPLIKQRRDFLTRIMTTSLSRTHL

Target ORF information:

RefSeq Version	XM_021466811.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio thymocyte selection associated family member 2 (themis2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021466811.1

ORF Insert Sequence:

ATGGGAGACC TCCAGTCCCT GCAAGCCTTC ATCAACTGTC TGGATCAGCG CTCTCTGCCC 
CGCATCCTTC AGGTCTGCTC GGGGGTTTAT TTTCAAGGGT CTGTGTATGA GATTTCGGGG 
AGTGAAGTGT GTTTATCCAC TGGAGATTTG GTGAAGATCA TTGGTTTGGA GCTCCTGTCT 
GTGTCTTGTG AAGATGTCAA CACGGGAGAT TCATTTGTAT TGCCTATTCA CTACACAGGT 
AATTTCAGAC TCGTCGCTGA AGACCTACCT TATAACACAG TGGAAGAGTT GGTGGGTTTG 
TGTCCAGTCG GAGTGGACGC CTGCGGCTCC TTCACCTTCA GAAGCACACA AGAGCTGATA 
GTGGACAACT TCACGCTGGC CCCAGACGAA CATTTGTCCC TGCTTTCGGT GGAGGTCTCT 
GAGGATGGAG AAAGGCTGGC TCGGTGTCAG GTGTTAGGGC ATAACATGGC CTACGCCGAG 
CTTCTGCTTC CTCTGTCTCT GAAAGGAGAG TTTTACGAGT GTGAGGATGA GCGCGGATAC 
TTTCTGCAGG AGATCATGTC CTCGGCCAGA CTCAGCTGCC GCCGCTTCTG CAACACAAAC 
ACCAAGAGCA GTGGCGGATT GCTGATGTTC AGCCCGGTCT ATGAAATCAG CGCCATTATG 
CATCTAAGGA AGAACATAGT CAAGTTTCCC TCCACCCTAG AAGTAGATGT CCAGGACGTG 
ACGGACCAAT TCCAGGATTT AGTCTTCATA ACTCCACTAT CACTGCCTGA AGTCGTGAGC 
CAGCCCAAAG AGTCTTTCCC GACAATGGCA GAAATCCTGG AAGCTCCAGA AGGTAACCAG 
TACTTCAGCT GCAGCTGGTT TGGTGGACTA CAAAAAGGCA AGAGCCTCAT ACTCCACGAA 
TGTCACCAAA AAACAATGAT ATTAGCCTCG ACTCCAAAGG GGAAGAAGGG AAAACAGTAC 
TTCATGATCT CCGACGACTA TGGAGGTCGC ATGAGGAGAC GCGCTCGGGA GTTCGGCTCA 
GTCTACGAGT TATATTTGGC GTCTTCTCAA TCTCCAGGAC TCAAAGTAAG CGTGAGCCGG 
GACTATGAAG CCGATGAGGA CGAGGGAATG CCTGCGCTCG GTGTCGGAGA AAATCTAGAG 
GTTTTGGGGA TGAGTTTGAT GGATAGACTC AAACCTGGAG CTGCTGAGAA AGTAGAAAGT 
CTAATTTGTA AACGGACTCA TGAGGTGGAC GATGATGATG ATGATGATGA AGATGATAGT 
GAGGAAATCT CAATGCCGCT GTTTATGGCG GGACACTTTG TGGAGAAGCT TTCGGATAAT 
AAGAAGTATC GATTAAACGA TTTAGTGAGC GGCTCTTTAC CGGTTGATGT CAAAGTTGTG 
ACTCGGGATA AAGATCTGGA GAAGGATCCG CTGATGGGGT TACCAGCGTT AAAGCTGGAG 
GAGACATTCA CTGAGACCAC CGTGTTGACG AGTCTACCCC ATAATCCAGA TTGGTGCTTT 
GAGTTGCCGG TTCGTTGGTT GCAAATGTCA GTCTGTTTCA CGTCTGATGA TCTGCCGTGG 
TCTCGGCCTC CAGAGCTTCA CGTTGAAACG GTCACCGAGA TGACGGAGAA ATTCTATTAC 
GAATATCACA AACTCATTGG CAAAACTGAG GAGCCGCCTC CACGTCCACC AAAAAGGAAG 
AAGTCCAGTT GTGACGCAAA CAAAAAGGTT CAATCTTTAC CGAGCTTGGG GAGTTCAGTG 
AGCAAACAAC TGGACAAACT TTCGTTGGGT CCGAAAGTAG AAAAACGCTC CGTTCCTCCA 
CCTCCGCAAG ACGAGACCCT ACATCAGCCT CCTCCGCTGT TGCCTAGAAA GAGCATATCG 
CTAGCAGAAA GTTCCAGTAT GCCCAACATG TATGTGAAAA GCCCTAGAAT GTCTCACAAA 
TCAGGTACGT TTACGGATTA CATTCAGATA CTAGTGGTTC TAAATAAGAT GTATACTTTA 
ACCTTTTCTT ATCTTCCTCT AATAAAACAG CGAAGAGACT TTCTGACTCG GATCATGACT 
ACGAGTCTAT CGAGGACGCA TTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THEMIS2 ( XM_005157857.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005157914.1 Danio rerio thymocyte selection associated family member 2 (themis2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa21048
Clone ID Related Accession (Same CDS sequence)	XM_005157857.4 , XM_005157857.3
Accession Version	XM_005157857.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	protein THEMIS2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005157857.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGACC TCCAGTCCCT GCAAGCCTTC ATCAACTGTC TGGATCAGCG CTCTCTGCCC 
CGCATCCTTC AGGTCTGCTC GGGGGTTTAT TTTCAAGGGT CTGTGTATGA GATTTCGGGG 
AGTGAAGTGT GTTTATCCAC TGGAGATTTG GTGAAGATCA TTGGTTTGGA GCTCCTGTCT 
GTGTCTTGTG AAGATGTCAA CACGGGAGAT TCATTTGTAT TGCCTATTCA CTACACAGGT 
AATTTCAGAC TCGTCGCTGA AGACCTACCT TATAACACAG TGGAAGAGTT GGTGGGTTTG 
TGTCCAGTCG GAGTGGACGC CTGCGGCTCC TTCACCTTCA GAAGCACACA AGAGCTGATA 
GTGGACAACT TCACGCTGGC CCCAGACGAA CATTTGTCCC TGCTTTCGGT GGAGGTCTCT 
GAGGATGGAG AAAGGCTGGC TCGGTGTCAG GTGTTAGGGC ATAACATGGC CTACGCCGAG 
CTTCTGCTTC CTCTGTCTCT GAAAGGAGAG TTTTACGAGT GTGAGGATGA GCGCGGATAC 
TTTCTGCAGG AGATCATGTC CTCGGCCAGA CTCAGCTGCC GCCGCTTCTG CAACACAAAC 
ACCAAGAGCA GTGGCGGATT GCTGATGTTC AGCCCGGTCT ATGAAATCAG CGCCATTATG 
CATCTAAGGA AGAACATAGT CAAGTTTCCC TCCACCCTAG AAGTAGATGT CCAGGACGTG 
ACGGACCAAT TCCAGGATTT AGTCTTCATA ACTCCACTAT CACTGCCTGA AGTCGTGAGC 
CAGCCCAAAG AGTCTTTCCC GACAATGGCA GAAATCCTGG AAGCTCCAGA AGGTAACCAG 
TACTTCAGCT GCAGCTGGTT TGGTGGACTA CAAAAAGGCA AGAGCCTCAT ACTCCACGAA 
TGTCACCAAA AAACAATGAT ATTAGCCTCG ACTCCAAAGG GGAAGAAGGG AAAACAGTAC 
TTCATGATCT CCGACGACTA TGGAGGTCGC ATGAGGAGAC GCGCTCGGGA GTTCGGCTCA 
GTCTACGAGT TATATTTGGC GTCTTCTCAA TCTCCAGGAC TCAAAGTAAG CGTGAGCCGG 
GACTATGAAG CCGATGAGGA CGAGGGAATG CCTGCGCTCG GTGTCGGAGA AAATCTAGAG 
GTTTTGGGGA TGAGTTTGAT GGATAGACTC AAACCTGGAG CTGCTGAGAA AGTAGAAAGT 
CTAATTTGTA AACGGACTCA TGAGGTGGAC GATGATGATG ATGATGATGA AGATGATAGT 
GAGGAAATCT CAATGCCGCT GTTTATGGCG GGACACTTTG TGGAGAAGCT TTCGGATAAT 
AAGAAGTATC GATTAAACGA TTTAGTGAGC GGCTCTTTAC CGGTTGATGT CAAAGTTGTG 
ACTCGGGATA AAGATCTGGA GAAGGATCCG CTGATGGGGT TACCAGCGTT AAAGCTGGAG 
GAGACATTCA CTGAGACCAC CGTGTTGACG AGTCTACCCC ATAATCCAGA TTGGTGCTTT 
GAGTTGCCGG TTCGTTGGTT GCAAATGTCA GTCTGTTTCA CGTCTGATGA TCTGCCGTGG 
TCTCGGCCTC CAGAGCTTCA CGTTGAAACG GTCACCGAGA TGACGGAGAA ATTCTATTAC 
GAATATCACA AACTCATTGG CAAAACTGAG GAGCCGCCTC CACGTCCACC AAAAAGGAAG 
AAGTCCAGTT GTGACGCAAA CAAAAAGGTT CAATCTTTAC CGAGCTTGGG GAGTTCAGTG 
AGCAAACAAC TGGACAAACT TTCGTTGGGT CCGAAAGTAG AAAAACGCTC CGTTCCTCCA 
CCTCCGCAAG ACGAGCAGAC CCTACATCAG CCTCCTCCGC TGTTGCCTAG AAAGAGCATA 
TCGCTAGCAG AAAGTTCCAG TATGCCCAAC ATGTATGTGA AAAGCCCTAG AATGTCTCAC 
AAATCAGCGA AGAGACTTTC TGACTCGGAT CATGACTACG AGTCTATCGA GGACGCATTT 
ATAAAACCCC ATGAAAGCCT GTACTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157914.1
CDS	406..2415
Translation	MGDLQSLQAFINCLDQRSLPRILQVCSGVYFQGSVYEISGSEVCLSTGDLVKIIGLELLSVSCEDVNTGDSFVLPIHYTGNFRLVAEDLPYNTVEELVGLCPVGVDACGSFTFRSTQELIVDNFTLAPDEHLSLLSVEVSEDGERLARCQVLGHNMAYAELLLPLSLKGEFYECEDERGYFLQEIMSSARLSCRRFCNTNTKSSGGLLMFSPVYEISAIMHLRKNIVKFPSTLEVDVQDVTDQFQDLVFITPLSLPEVVSQPKESFPTMAEILEAPEGNQYFSCSWFGGLQKGKSLILHECHQKTMILASTPKGKKGKQYFMISDDYGGRMRRRAREFGSVYELYLASSQSPGLKVSVSRDYEADEDEGMPALGVGENLEVLGMSLMDRLKPGAAEKVESLICKRTHEVDDDDDDDEDDSEEISMPLFMAGHFVEKLSDNKKYRLNDLVSGSLPVDVKVVTRDKDLEKDPLMGLPALKLEETFTETTVLTSLPHNPDWCFELPVRWLQMSVCFTSDDLPWSRPPELHVETVTEMTEKFYYEYHKLIGKTEEPPPRPPKRKKSSCDANKKVQSLPSLGSSVSKQLDKLSLGPKVEKRSVPPPPQDEQTLHQPPPLLPRKSISLAESSSMPNMYVKSPRMSHKSAKRLSDSDHDYESIEDAFIKPHESLYY

Target ORF information:

RefSeq Version	XM_005157857.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio thymocyte selection associated family member 2 (themis2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157857.4

ORF Insert Sequence:

ATGGGAGACC TCCAGTCCCT GCAAGCCTTC ATCAACTGTC TGGATCAGCG CTCTCTGCCC 
CGCATCCTTC AGGTCTGCTC GGGGGTTTAT TTTCAAGGGT CTGTGTATGA GATTTCGGGG 
AGTGAAGTGT GTTTATCCAC TGGAGATTTG GTGAAGATCA TTGGTTTGGA GCTCCTGTCT 
GTGTCTTGTG AAGATGTCAA CACGGGAGAT TCATTTGTAT TGCCTATTCA CTACACAGGT 
AATTTCAGAC TCGTCGCTGA AGACCTACCT TATAACACAG TGGAAGAGTT GGTGGGTTTG 
TGTCCAGTCG GAGTGGACGC CTGCGGCTCC TTCACCTTCA GAAGCACACA AGAGCTGATA 
GTGGACAACT TCACGCTGGC CCCAGACGAA CATTTGTCCC TGCTTTCGGT GGAGGTCTCT 
GAGGATGGAG AAAGGCTGGC TCGGTGTCAG GTGTTAGGGC ATAACATGGC CTACGCCGAG 
CTTCTGCTTC CTCTGTCTCT GAAAGGAGAG TTTTACGAGT GTGAGGATGA GCGCGGATAC 
TTTCTGCAGG AGATCATGTC CTCGGCCAGA CTCAGCTGCC GCCGCTTCTG CAACACAAAC 
ACCAAGAGCA GTGGCGGATT GCTGATGTTC AGCCCGGTCT ATGAAATCAG CGCCATTATG 
CATCTAAGGA AGAACATAGT CAAGTTTCCC TCCACCCTAG AAGTAGATGT CCAGGACGTG 
ACGGACCAAT TCCAGGATTT AGTCTTCATA ACTCCACTAT CACTGCCTGA AGTCGTGAGC 
CAGCCCAAAG AGTCTTTCCC GACAATGGCA GAAATCCTGG AAGCTCCAGA AGGTAACCAG 
TACTTCAGCT GCAGCTGGTT TGGTGGACTA CAAAAAGGCA AGAGCCTCAT ACTCCACGAA 
TGTCACCAAA AAACAATGAT ATTAGCCTCG ACTCCAAAGG GGAAGAAGGG AAAACAGTAC 
TTCATGATCT CCGACGACTA TGGAGGTCGC ATGAGGAGAC GCGCTCGGGA GTTCGGCTCA 
GTCTACGAGT TATATTTGGC GTCTTCTCAA TCTCCAGGAC TCAAAGTAAG CGTGAGCCGG 
GACTATGAAG CCGATGAGGA CGAGGGAATG CCTGCGCTCG GTGTCGGAGA AAATCTAGAG 
GTTTTGGGGA TGAGTTTGAT GGATAGACTC AAACCTGGAG CTGCTGAGAA AGTAGAAAGT 
CTAATTTGTA AACGGACTCA TGAGGTGGAC GATGATGATG ATGATGATGA AGATGATAGT 
GAGGAAATCT CAATGCCGCT GTTTATGGCG GGACACTTTG TGGAGAAGCT TTCGGATAAT 
AAGAAGTATC GATTAAACGA TTTAGTGAGC GGCTCTTTAC CGGTTGATGT CAAAGTTGTG 
ACTCGGGATA AAGATCTGGA GAAGGATCCG CTGATGGGGT TACCAGCGTT AAAGCTGGAG 
GAGACATTCA CTGAGACCAC CGTGTTGACG AGTCTACCCC ATAATCCAGA TTGGTGCTTT 
GAGTTGCCGG TTCGTTGGTT GCAAATGTCA GTCTGTTTCA CGTCTGATGA TCTGCCGTGG 
TCTCGGCCTC CAGAGCTTCA CGTTGAAACG GTCACCGAGA TGACGGAGAA ATTCTATTAC 
GAATATCACA AACTCATTGG CAAAACTGAG GAGCCGCCTC CACGTCCACC AAAAAGGAAG 
AAGTCCAGTT GTGACGCAAA CAAAAAGGTT CAATCTTTAC CGAGCTTGGG GAGTTCAGTG 
AGCAAACAAC TGGACAAACT TTCGTTGGGT CCGAAAGTAG AAAAACGCTC CGTTCCTCCA 
CCTCCGCAAG ACGAGCAGAC CCTACATCAG CCTCCTCCGC TGTTGCCTAG AAAGAGCATA 
TCGCTAGCAG AAAGTTCCAG TATGCCCAAC ATGTATGTGA AAAGCCCTAG AATGTCTCAC 
AAATCAGCGA AGAGACTTTC TGACTCGGAT CATGACTACG AGTCTATCGA GGACGCATTT 
ATAAAACCCC ATGAAAGCCT GTACTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THEMIS2 ( XM_005157857.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005157914.1 Danio rerio thymocyte selection associated family member 2 (themis2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa21048
Clone ID Related Accession (Same CDS sequence)	XM_005157857.4 , XM_005157857.3
Accession Version	XM_005157857.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	protein THEMIS2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005157857.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGACC TCCAGTCCCT GCAAGCCTTC ATCAACTGTC TGGATCAGCG CTCTCTGCCC 
CGCATCCTTC AGGTCTGCTC GGGGGTTTAT TTTCAAGGGT CTGTGTATGA GATTTCGGGG 
AGTGAAGTGT GTTTATCCAC TGGAGATTTG GTGAAGATCA TTGGTTTGGA GCTCCTGTCT 
GTGTCTTGTG AAGATGTCAA CACGGGAGAT TCATTTGTAT TGCCTATTCA CTACACAGGT 
AATTTCAGAC TCGTCGCTGA AGACCTACCT TATAACACAG TGGAAGAGTT GGTGGGTTTG 
TGTCCAGTCG GAGTGGACGC CTGCGGCTCC TTCACCTTCA GAAGCACACA AGAGCTGATA 
GTGGACAACT TCACGCTGGC CCCAGACGAA CATTTGTCCC TGCTTTCGGT GGAGGTCTCT 
GAGGATGGAG AAAGGCTGGC TCGGTGTCAG GTGTTAGGGC ATAACATGGC CTACGCCGAG 
CTTCTGCTTC CTCTGTCTCT GAAAGGAGAG TTTTACGAGT GTGAGGATGA GCGCGGATAC 
TTTCTGCAGG AGATCATGTC CTCGGCCAGA CTCAGCTGCC GCCGCTTCTG CAACACAAAC 
ACCAAGAGCA GTGGCGGATT GCTGATGTTC AGCCCGGTCT ATGAAATCAG CGCCATTATG 
CATCTAAGGA AGAACATAGT CAAGTTTCCC TCCACCCTAG AAGTAGATGT CCAGGACGTG 
ACGGACCAAT TCCAGGATTT AGTCTTCATA ACTCCACTAT CACTGCCTGA AGTCGTGAGC 
CAGCCCAAAG AGTCTTTCCC GACAATGGCA GAAATCCTGG AAGCTCCAGA AGGTAACCAG 
TACTTCAGCT GCAGCTGGTT TGGTGGACTA CAAAAAGGCA AGAGCCTCAT ACTCCACGAA 
TGTCACCAAA AAACAATGAT ATTAGCCTCG ACTCCAAAGG GGAAGAAGGG AAAACAGTAC 
TTCATGATCT CCGACGACTA TGGAGGTCGC ATGAGGAGAC GCGCTCGGGA GTTCGGCTCA 
GTCTACGAGT TATATTTGGC GTCTTCTCAA TCTCCAGGAC TCAAAGTAAG CGTGAGCCGG 
GACTATGAAG CCGATGAGGA CGAGGGAATG CCTGCGCTCG GTGTCGGAGA AAATCTAGAG 
GTTTTGGGGA TGAGTTTGAT GGATAGACTC AAACCTGGAG CTGCTGAGAA AGTAGAAAGT 
CTAATTTGTA AACGGACTCA TGAGGTGGAC GATGATGATG ATGATGATGA AGATGATAGT 
GAGGAAATCT CAATGCCGCT GTTTATGGCG GGACACTTTG TGGAGAAGCT TTCGGATAAT 
AAGAAGTATC GATTAAACGA TTTAGTGAGC GGCTCTTTAC CGGTTGATGT CAAAGTTGTG 
ACTCGGGATA AAGATCTGGA GAAGGATCCG CTGATGGGGT TACCAGCGTT AAAGCTGGAG 
GAGACATTCA CTGAGACCAC CGTGTTGACG AGTCTACCCC ATAATCCAGA TTGGTGCTTT 
GAGTTGCCGG TTCGTTGGTT GCAAATGTCA GTCTGTTTCA CGTCTGATGA TCTGCCGTGG 
TCTCGGCCTC CAGAGCTTCA CGTTGAAACG GTCACCGAGA TGACGGAGAA ATTCTATTAC 
GAATATCACA AACTCATTGG CAAAACTGAG GAGCCGCCTC CACGTCCACC AAAAAGGAAG 
AAGTCCAGTT GTGACGCAAA CAAAAAGGTT CAATCTTTAC CGAGCTTGGG GAGTTCAGTG 
AGCAAACAAC TGGACAAACT TTCGTTGGGT CCGAAAGTAG AAAAACGCTC CGTTCCTCCA 
CCTCCGCAAG ACGAGCAGAC CCTACATCAG CCTCCTCCGC TGTTGCCTAG AAAGAGCATA 
TCGCTAGCAG AAAGTTCCAG TATGCCCAAC ATGTATGTGA AAAGCCCTAG AATGTCTCAC 
AAATCAGCGA AGAGACTTTC TGACTCGGAT CATGACTACG AGTCTATCGA GGACGCATTT 
ATAAAACCCC ATGAAAGCCT GTACTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157914.1
CDS	308..2317
Translation	MGDLQSLQAFINCLDQRSLPRILQVCSGVYFQGSVYEISGSEVCLSTGDLVKIIGLELLSVSCEDVNTGDSFVLPIHYTGNFRLVAEDLPYNTVEELVGLCPVGVDACGSFTFRSTQELIVDNFTLAPDEHLSLLSVEVSEDGERLARCQVLGHNMAYAELLLPLSLKGEFYECEDERGYFLQEIMSSARLSCRRFCNTNTKSSGGLLMFSPVYEISAIMHLRKNIVKFPSTLEVDVQDVTDQFQDLVFITPLSLPEVVSQPKESFPTMAEILEAPEGNQYFSCSWFGGLQKGKSLILHECHQKTMILASTPKGKKGKQYFMISDDYGGRMRRRAREFGSVYELYLASSQSPGLKVSVSRDYEADEDEGMPALGVGENLEVLGMSLMDRLKPGAAEKVESLICKRTHEVDDDDDDDEDDSEEISMPLFMAGHFVEKLSDNKKYRLNDLVSGSLPVDVKVVTRDKDLEKDPLMGLPALKLEETFTETTVLTSLPHNPDWCFELPVRWLQMSVCFTSDDLPWSRPPELHVETVTEMTEKFYYEYHKLIGKTEEPPPRPPKRKKSSCDANKKVQSLPSLGSSVSKQLDKLSLGPKVEKRSVPPPPQDEQTLHQPPPLLPRKSISLAESSSMPNMYVKSPRMSHKSAKRLSDSDHDYESIEDAFIKPHESLYY

Target ORF information:

RefSeq Version	XM_005157857.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio thymocyte selection associated family member 2 (themis2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157857.3

ORF Insert Sequence:

ATGGGAGACC TCCAGTCCCT GCAAGCCTTC ATCAACTGTC TGGATCAGCG CTCTCTGCCC 
CGCATCCTTC AGGTCTGCTC GGGGGTTTAT TTTCAAGGGT CTGTGTATGA GATTTCGGGG 
AGTGAAGTGT GTTTATCCAC TGGAGATTTG GTGAAGATCA TTGGTTTGGA GCTCCTGTCT 
GTGTCTTGTG AAGATGTCAA CACGGGAGAT TCATTTGTAT TGCCTATTCA CTACACAGGT 
AATTTCAGAC TCGTCGCTGA AGACCTACCT TATAACACAG TGGAAGAGTT GGTGGGTTTG 
TGTCCAGTCG GAGTGGACGC CTGCGGCTCC TTCACCTTCA GAAGCACACA AGAGCTGATA 
GTGGACAACT TCACGCTGGC CCCAGACGAA CATTTGTCCC TGCTTTCGGT GGAGGTCTCT 
GAGGATGGAG AAAGGCTGGC TCGGTGTCAG GTGTTAGGGC ATAACATGGC CTACGCCGAG 
CTTCTGCTTC CTCTGTCTCT GAAAGGAGAG TTTTACGAGT GTGAGGATGA GCGCGGATAC 
TTTCTGCAGG AGATCATGTC CTCGGCCAGA CTCAGCTGCC GCCGCTTCTG CAACACAAAC 
ACCAAGAGCA GTGGCGGATT GCTGATGTTC AGCCCGGTCT ATGAAATCAG CGCCATTATG 
CATCTAAGGA AGAACATAGT CAAGTTTCCC TCCACCCTAG AAGTAGATGT CCAGGACGTG 
ACGGACCAAT TCCAGGATTT AGTCTTCATA ACTCCACTAT CACTGCCTGA AGTCGTGAGC 
CAGCCCAAAG AGTCTTTCCC GACAATGGCA GAAATCCTGG AAGCTCCAGA AGGTAACCAG 
TACTTCAGCT GCAGCTGGTT TGGTGGACTA CAAAAAGGCA AGAGCCTCAT ACTCCACGAA 
TGTCACCAAA AAACAATGAT ATTAGCCTCG ACTCCAAAGG GGAAGAAGGG AAAACAGTAC 
TTCATGATCT CCGACGACTA TGGAGGTCGC ATGAGGAGAC GCGCTCGGGA GTTCGGCTCA 
GTCTACGAGT TATATTTGGC GTCTTCTCAA TCTCCAGGAC TCAAAGTAAG CGTGAGCCGG 
GACTATGAAG CCGATGAGGA CGAGGGAATG CCTGCGCTCG GTGTCGGAGA AAATCTAGAG 
GTTTTGGGGA TGAGTTTGAT GGATAGACTC AAACCTGGAG CTGCTGAGAA AGTAGAAAGT 
CTAATTTGTA AACGGACTCA TGAGGTGGAC GATGATGATG ATGATGATGA AGATGATAGT 
GAGGAAATCT CAATGCCGCT GTTTATGGCG GGACACTTTG TGGAGAAGCT TTCGGATAAT 
AAGAAGTATC GATTAAACGA TTTAGTGAGC GGCTCTTTAC CGGTTGATGT CAAAGTTGTG 
ACTCGGGATA AAGATCTGGA GAAGGATCCG CTGATGGGGT TACCAGCGTT AAAGCTGGAG 
GAGACATTCA CTGAGACCAC CGTGTTGACG AGTCTACCCC ATAATCCAGA TTGGTGCTTT 
GAGTTGCCGG TTCGTTGGTT GCAAATGTCA GTCTGTTTCA CGTCTGATGA TCTGCCGTGG 
TCTCGGCCTC CAGAGCTTCA CGTTGAAACG GTCACCGAGA TGACGGAGAA ATTCTATTAC 
GAATATCACA AACTCATTGG CAAAACTGAG GAGCCGCCTC CACGTCCACC AAAAAGGAAG 
AAGTCCAGTT GTGACGCAAA CAAAAAGGTT CAATCTTTAC CGAGCTTGGG GAGTTCAGTG 
AGCAAACAAC TGGACAAACT TTCGTTGGGT CCGAAAGTAG AAAAACGCTC CGTTCCTCCA 
CCTCCGCAAG ACGAGCAGAC CCTACATCAG CCTCCTCCGC TGTTGCCTAG AAAGAGCATA 
TCGCTAGCAG AAAGTTCCAG TATGCCCAAC ATGTATGTGA AAAGCCCTAG AATGTCTCAC 
AAATCAGCGA AGAGACTTTC TGACTCGGAT CATGACTACG AGTCTATCGA GGACGCATTT 
ATAAAACCCC ATGAAAGCCT GTACTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THEMIS2 ( XM_009292033.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009290308.1 Danio rerio thymocyte selection associated family member 2 (themis2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa50202
Clone ID Related Accession (Same CDS sequence)	XM_009292033.2
Accession Version	XM_009292033.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	protein THEMIS2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292033.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGACC TCCAGTCCCT GCAAGCCTTC ATCAACTGTC TGGATCAGCG CTCTCTGCCC 
CGCATCCTTC AGGTCTGCTC GGGGGTTTAT TTTCAAGGGT CTGTGTATGA GATTTCGGGG 
AGTGAAGTGT GTTTATCCAC TGGAGATTTG GTGAAGATCA TTGGTTTGGA GCTCCTGTCT 
GTGTCTTGTG AAGATGTCAA CACGGGAGAT TCATTTGTAT TGCCTATTCA CTACACAGGT 
AATTTCAGAC TCGTCGCTGA AGACCTACCT TATAACACAG TGGAAGAGTT GGTGGGTTTG 
TGTCCAGTCG GAGTGGACGC CTGCGGCTCC TTCACCTTCA GAAGCACACA AGAGCTGATA 
GTGGACAACT TCACGCTGGC CCCAGACGAA CATTTGTCCC TGCTTTCGGT GGAGGTCTCT 
GAGGATGGAG AAAGGCTGGC TCGGTGTCAG GTGTTAGGGC ATAACATGGC CTACGCCGAG 
CTTCTGCTTC CTCTGTCTCT GAAAGGAGAG TTTTACGAGT GTGAGGATGA GCGCGGATAC 
TTTCTGCAGG AGATCATGTC CTCGGCCAGA CTCAGCTGCC GCCGCTTCTG CAACACAAAC 
ACCAAGAGCA GTGGCGGATT GCTGATGTTC AGCCCGGTCT ATGAAATCAG CGCCATTATG 
CATCTAAGGA AGAACATAGT CAAGTTTCCC TCCACCCTAG AAGTAGATGT CCAGGACGTG 
ACGGACCAAT TCCAGGATTT AGTCTTCATA ACTCCACTAT CACTGCCTGA AGTCGTGAGC 
CAGCCCAAAG AGTCTTTCCC GACAATGGCA GAAATCCTGG AAGCTCCAGA AGGTAACCAG 
TACTTCAGCT GCAGCTGGTT TGGTGGACTA CAAAAAGGCA AGAGCCTCAT ACTCCACGAA 
TGTCACCAAA AAACAATGAT ATTAGCCTCG ACTCCAAAGG GGAAGAAGGG AAAACAGTAC 
TTCATGATCT CCGACGACTA TGGAGGTCGC ATGAGGAGAC GCGCTCGGGA GTTCGGCTCA 
GTCTACGAGT TATATTTGGC GTCTTCTCAA TCTCCAGGAC TCAAAGTAAG CGTGAGCCGG 
GACTATGAAG CCGATGAGGA CGAGGGAATG CCTGCGCTCG GTGTCGGAGA AAATCTAGAG 
GTTTTGGGGA TGAGTTTGAT GGATAGACTC AAACCTGGAG CTGCTGAGAA AGTAGAAAGT 
CTAATTTGTA AACGGACTCA TGAGGTGGAC GATGATGATG ATGATGATGA AGATGATAGT 
GAGGAAATCT CAATGCCGCT GTTTATGGCG GGACACTTTG TGGAGAAGCT TTCGGATAAT 
AAGAAGTATC GATTAAACGA TTTAGTGAGC GGCTCTTTAC CGGTTGATGT CAAAGTTGTG 
ACTCGGGATA AAGATCTGGA GAAGGATCCG CTGATGGGGT TACCAGCGTT AAAGCTGGAG 
GAGACATTCA CTGAGACCAC CGTGTTGACG AGTCTACCCC ATAATCCAGA TTGGTGCTTT 
GAGTTGCCGG TTCGTTGGTT GCAAATGTCA GTCTGTTTCA CGTCTGATGA TCTGCCGTGG 
TCTCGGCCTC CAGAGCTTCA CGTTGAAACG GTCACCGAGA TGACGGAGAA ATTCTATTAC 
GAATATCACA AACTCATTGG CAAAACTGAG GAGCCGCCTC CACGTCCACC AAAAAGGAAG 
AAGTCCAGTT GTGACGCAAA CAAAAAGGTT CAATCTTTAC CGAGCTTGGG GAGTTCAGTG 
AGCAAACAAC TGGACAAACT TTCGTTGGGT CCGAAAGTAG AAAAACGCTC CGTTCCTCCA 
CCTCCGCAAG ACGAGACCCT ACATCAGCCT CCTCCGCTGT TGCCTAGAAA GAGCATATCG 
CTAGCAGAAA GTTCCAGTAT GCCCAACATG TATGTGAAAA GCCCTAGAAT GTCTCACAAA 
TCAGCGAAGA GACTTTCTGA CTCGGATCAT GACTACGAGT CTATCGAGGA CGCATTTATA 
AAACCCCATG AAAGCCTGTA CTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290308.1
CDS	308..2314
Translation	MGDLQSLQAFINCLDQRSLPRILQVCSGVYFQGSVYEISGSEVCLSTGDLVKIIGLELLSVSCEDVNTGDSFVLPIHYTGNFRLVAEDLPYNTVEELVGLCPVGVDACGSFTFRSTQELIVDNFTLAPDEHLSLLSVEVSEDGERLARCQVLGHNMAYAELLLPLSLKGEFYECEDERGYFLQEIMSSARLSCRRFCNTNTKSSGGLLMFSPVYEISAIMHLRKNIVKFPSTLEVDVQDVTDQFQDLVFITPLSLPEVVSQPKESFPTMAEILEAPEGNQYFSCSWFGGLQKGKSLILHECHQKTMILASTPKGKKGKQYFMISDDYGGRMRRRAREFGSVYELYLASSQSPGLKVSVSRDYEADEDEGMPALGVGENLEVLGMSLMDRLKPGAAEKVESLICKRTHEVDDDDDDDEDDSEEISMPLFMAGHFVEKLSDNKKYRLNDLVSGSLPVDVKVVTRDKDLEKDPLMGLPALKLEETFTETTVLTSLPHNPDWCFELPVRWLQMSVCFTSDDLPWSRPPELHVETVTEMTEKFYYEYHKLIGKTEEPPPRPPKRKKSSCDANKKVQSLPSLGSSVSKQLDKLSLGPKVEKRSVPPPPQDETLHQPPPLLPRKSISLAESSSMPNMYVKSPRMSHKSAKRLSDSDHDYESIEDAFIKPHESLYY

Target ORF information:

RefSeq Version	XM_009292033.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio thymocyte selection associated family member 2 (themis2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009292033.2

ORF Insert Sequence:

ATGGGAGACC TCCAGTCCCT GCAAGCCTTC ATCAACTGTC TGGATCAGCG CTCTCTGCCC 
CGCATCCTTC AGGTCTGCTC GGGGGTTTAT TTTCAAGGGT CTGTGTATGA GATTTCGGGG 
AGTGAAGTGT GTTTATCCAC TGGAGATTTG GTGAAGATCA TTGGTTTGGA GCTCCTGTCT 
GTGTCTTGTG AAGATGTCAA CACGGGAGAT TCATTTGTAT TGCCTATTCA CTACACAGGT 
AATTTCAGAC TCGTCGCTGA AGACCTACCT TATAACACAG TGGAAGAGTT GGTGGGTTTG 
TGTCCAGTCG GAGTGGACGC CTGCGGCTCC TTCACCTTCA GAAGCACACA AGAGCTGATA 
GTGGACAACT TCACGCTGGC CCCAGACGAA CATTTGTCCC TGCTTTCGGT GGAGGTCTCT 
GAGGATGGAG AAAGGCTGGC TCGGTGTCAG GTGTTAGGGC ATAACATGGC CTACGCCGAG 
CTTCTGCTTC CTCTGTCTCT GAAAGGAGAG TTTTACGAGT GTGAGGATGA GCGCGGATAC 
TTTCTGCAGG AGATCATGTC CTCGGCCAGA CTCAGCTGCC GCCGCTTCTG CAACACAAAC 
ACCAAGAGCA GTGGCGGATT GCTGATGTTC AGCCCGGTCT ATGAAATCAG CGCCATTATG 
CATCTAAGGA AGAACATAGT CAAGTTTCCC TCCACCCTAG AAGTAGATGT CCAGGACGTG 
ACGGACCAAT TCCAGGATTT AGTCTTCATA ACTCCACTAT CACTGCCTGA AGTCGTGAGC 
CAGCCCAAAG AGTCTTTCCC GACAATGGCA GAAATCCTGG AAGCTCCAGA AGGTAACCAG 
TACTTCAGCT GCAGCTGGTT TGGTGGACTA CAAAAAGGCA AGAGCCTCAT ACTCCACGAA 
TGTCACCAAA AAACAATGAT ATTAGCCTCG ACTCCAAAGG GGAAGAAGGG AAAACAGTAC 
TTCATGATCT CCGACGACTA TGGAGGTCGC ATGAGGAGAC GCGCTCGGGA GTTCGGCTCA 
GTCTACGAGT TATATTTGGC GTCTTCTCAA TCTCCAGGAC TCAAAGTAAG CGTGAGCCGG 
GACTATGAAG CCGATGAGGA CGAGGGAATG CCTGCGCTCG GTGTCGGAGA AAATCTAGAG 
GTTTTGGGGA TGAGTTTGAT GGATAGACTC AAACCTGGAG CTGCTGAGAA AGTAGAAAGT 
CTAATTTGTA AACGGACTCA TGAGGTGGAC GATGATGATG ATGATGATGA AGATGATAGT 
GAGGAAATCT CAATGCCGCT GTTTATGGCG GGACACTTTG TGGAGAAGCT TTCGGATAAT 
AAGAAGTATC GATTAAACGA TTTAGTGAGC GGCTCTTTAC CGGTTGATGT CAAAGTTGTG 
ACTCGGGATA AAGATCTGGA GAAGGATCCG CTGATGGGGT TACCAGCGTT AAAGCTGGAG 
GAGACATTCA CTGAGACCAC CGTGTTGACG AGTCTACCCC ATAATCCAGA TTGGTGCTTT 
GAGTTGCCGG TTCGTTGGTT GCAAATGTCA GTCTGTTTCA CGTCTGATGA TCTGCCGTGG 
TCTCGGCCTC CAGAGCTTCA CGTTGAAACG GTCACCGAGA TGACGGAGAA ATTCTATTAC 
GAATATCACA AACTCATTGG CAAAACTGAG GAGCCGCCTC CACGTCCACC AAAAAGGAAG 
AAGTCCAGTT GTGACGCAAA CAAAAAGGTT CAATCTTTAC CGAGCTTGGG GAGTTCAGTG 
AGCAAACAAC TGGACAAACT TTCGTTGGGT CCGAAAGTAG AAAAACGCTC CGTTCCTCCA 
CCTCCGCAAG ACGAGACCCT ACATCAGCCT CCTCCGCTGT TGCCTAGAAA GAGCATATCG 
CTAGCAGAAA GTTCCAGTAT GCCCAACATG TATGTGAAAA GCCCTAGAAT GTCTCACAAA 
TCAGCGAAGA GACTTTCTGA CTCGGATCAT GACTACGAGT CTATCGAGGA CGCATTTATA 
AAACCCCATG AAAGCCTGTA CTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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THEMIS2 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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