itga3b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	itga3b
Entrez Gene ID	100331874
Full Name	integrin, alpha 3b
Synonyms	im:7145180,im:7155971
General protein information	Preferred Names integrin, alpha 3b Names integrin alpha-3 badfin bdf frayed fyd integrin alpha 3 itga3 unm tz296 unm_tz296
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009306420.2	XP_009304695.1	integrin alpha-3 isoform X1
NM_001190309.1	NP_001177238.1	integrin alpha-3 precursor
XM_009306420.3	XP_009304695.1	integrin alpha-3 isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	itga3a	XP_005164152.1
Danio rerio (zebrafish)	itga3b	NP_001177238.1

Gene Ontology
Bibliography

Related articles in PubMed

Focal adhesions are essential to drive zebrafish heart valve morphogenesis.
Gunawan F, Gentile A, Fukuda R, Tsedeke AT, Jiménez-Amilburu V, Ramadass R, Iida A, Sehara-Fujisawa A, Stainier DYR
The Journal of cell biology218(3)1039-1054(2019 03)

Deficiency of lrp4 in zebrafish and human LRP4 mutation induce aberrant activation of Jagged-Notch signaling in fin and limb development.
Tian J, Shao J, Liu C, Hou HY, Chou CW, Shboul M, Li GQ, El-Khateeb M, Samarah OQ, Kou Y, Chen YH, Chen MJ, Lyu Z, Chen WL, Chen YF, Sun YH, Liu YW
Cellular and molecular life sciences : CMLS76(1)163-178(2019 Jan)

Gon4l regulates notochord boundary formation and cell polarity underlying axis extension by repressing adhesion genes.
Williams MLK, Sawada A, Budine T, Yin C, Gontarz P, Solnica-Krezel L
Nature communications9(1)1319(2018 04)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Zebrafish Collagen Type I: Molecular and Biochemical Characterization of the Major Structural Protein in Bone and Skin.
Gistelinck C, Gioia R, Gagliardi A, Tonelli F, Marchese L, Bianchi L, Landi C, Bini L, Huysseune A, Witten PE, Staes A, Gevaert K, De Rocker N, Menten B, Malfait F, Leikin S, Carra S, Tenni R, Rossi A, De Paepe A, Coucke P, Willaert A, Forlino A
Scientific reports621540(2016 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following itga3b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the itga3b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa38887	NM_001190309.1 Latest version!	Danio rerio integrin, alpha 3b (itga3b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa49540	XM_009306420.3 Latest version!	Danio rerio integrin, alpha 3b (itga3b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
ODa49540	XM_009306420.2	Danio rerio integrin, alpha 3b (itga3b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

itga3b ( NM_001190309.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001177238.1 Danio rerio integrin, alpha 3b (itga3b), mRNA.

Price & Availability↑

CloneID	ODa38887
Clone ID Related Accession (Same CDS sequence)	NM_001190309.1
Accession Version	NM_001190309.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562860800000
Organism	Danio rerio(zebrafish)
Product	integrin alpha-3 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from GU936669.1. On Jun 16, 2010 this sequence version replaced XM_002663911.1. ##Evidence-Data-START## Transcript exon combination :: GU936669.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCCGGAA AGTCTCTGCA CTTGTGCGTC TTCGTAATTT GCGCAATTCA AACGAACACC 
GGATTTAACA TTGACGTGCA GTTCCCGGTT ATCAAAGAAG GAAAAACCAA GGGCAGTCTA 
TTTGGATTCT CAGTGGCACT GCACAAACAG ACCGAAAAGG CGAAGAAGAA CTTGCTGTTA 
GTCGGAGCCC CACAAGAAAA AGCCCAGCCT CAACTCAGTA AATTCAAAAT TAATGAAACT 
GGAGCCGTGT ATTATTGTCC CATCACTGTT AACCTGGATG ACTGTGGAAG CATGGACCTC 
ATCAGTCCAC CAAAGTCGAC GGATATGCTG GAGGGAATGT GGCTAGGAGT GACCGTGGCC 
AGTCAGAGGA ATGGAGGCCG TGTGCTGGCA TGTGGTCATC GCTATGTCAA AAAACTGCTG 
GGCGCAGAAG AGCAGCAGCG TATGGTGGGC AAATGTTACG TGCGGGGCAA TGACCTGAGC 
TACGACCCAT CTGATTACTG GCAGTCGGAA ACCTACGAGC TGTGCGACTT CAACCACGAC 
CAGGACCTGG AGGGCATGTG TAACATGGGT ATATCAGGAG GCATGACGGA GAAAGATGTG 
TATTTTGGGA CACCTGGCAG CTTCACGTGG CAGGGCACCG TCCATATGAA GTGCAGAGAC 
ACAGACTCCG ATTACGCTGG CGATGCCAAT GATAGGAGCT TTGGTAAACT GGGTGAGGAC 
AAGTTCAACA TCTACATAGG TTACTCTGTG CTGGAGGACA TAAAGTTACT GGATCGCTCT 
GTGTTCACTG TGGTGATGGG GGCTCCCAGA GATGAATTCA AGGGTTCAGT GATCCTGGCC 
GACAAACAAG ACTTGATACT CAAGCAGCTG ATGAATATCA CAGGGGAACA AATAGGGTCG 
TACTTTGGCA GCTGCTTGGC AGTAACAGAC CTCAACAATG ACGATTGGAA TGACCTGATC 
GTCGGCGCGC CATTTTATTT CGACCGCTAT AAGGAGGAAG GAGGTGCTGT TTACATCTTT 
ATGAACGAAA ACGGATCCTT CCAGAAAAAA GCCAGCCTGG TTCTGAAGGG TAAAAAGGGT 
TCTGGGTTTG GGTTTGCCAT TGCTGCGGTC GGAGATGTCA ATCAGGATGG ATTTCAAGAT 
ATTGCTGTCG GCGCTCCATT CCAGGACTCT GGAAAAGTCT ACATATGGAT GGGAAGTAAA 
AGTGGAATAA CTAAAGAACC CAGCCAGGTG ATCGAGGGTA AGTCATTGGG TCCCAGTGGT 
TTTCAGACGT TTGGTTATTC TCTCAGTGGA GGAATGGATA TGGATGGAAA TGATTATCCA 
GATATTTTGG TGGGCTCTTT GGATGACCGC ATCGCACTCT TGAGGTCTCG TCCGGTCATT 
CATCTCTCCA AAAACTTCAC CGTGGAGCCA AAGATTGTGA TTCCAAGCCA GTGTGGCAAT 
TCCTGCATAA AGGTAAAGGT GTGCTTTAGT TACACTCTCA GCAATGGAAA CAGCAACTTC 
AGGAAGGAAA TCACTGTGAA GTTTACGATA GATGCTGACC AGAATCGCAG AGGTGCTCGA 
GTACGCTTTA TAGACAACAA GCAAAGCACG TTTACAGGCC TTCTGTCTTT CTCTGCTTCT 
TCCTGTCAAG AGCTGGAGCT CAGTGTTAAT AATCCAGTGA CGGACAAACT TCAGCCCATA 
GTGTTCAGTC TGAACGTGTC TCTAAATCAG CAGAAAGCAG AAGCGCAGCA GACATTACAG 
AGTCTAGACT CTTTCCCTGT GCTGAGTGGA CAGCAGATCC TCACCGACAA AGCTGAGATT 
AATTTCCACA AGGAGTGTGG AGATGATAAC CGTTGTAGCA GTAACCTGCA GCTCAAGGCC 
AGGTTTGCAG ATGAAAACTA CAATCCCTTT CCCAGTCAGA CGCTGGAGTT TAGCAGTAAT 
GTGAAGAAGC TGGTGCTGAT GGTGGAGGTC TCCAACACAG CGGATGAGAA CAAACCGGCG 
GAGGATGCTC ATCAGGCCAC GCTCAACATC TCCATTCCAT ACTCACTCAA ATACTCAGGC 
GTCCGTCCTC CGGCTGGTTT TGATATGGAG TGCAGTGCAG ACGAGGCTGT CATATGTGAT 
CTGGGAAACC CATTTAAGAG CAATCAGAAG GTCTCATTTA TAATCATCTT TGAGACCTCT 
GGTATTACAC TTTATACAGA GCAGATCCAA TCTCAGCTGC AGCTCTCCAC TATTAGTGAA 
CAGGCGGATC TGTTTCCTGT TCTTGTCACC CTCAATGTGA AGAACACGAT CCTCACTACA 
TTTTCTTTGG AAAAGCTAAT AATCGACACG TCGTTCAGCG GCAATGTGAT TGGAGAATCT 
GCCATGAGCA GCACCAGTGA TGTGGGGAGT CCATTGGAGT TTGTGTTTCG TGTGCAGGTG 
CTGGGAGAAC CACTGGGAGA TCTGGGAACT CTTGTGATTG ACTTTGACTG GCCGTATGCA 
GTAGCCAATG GGAAATGGCT GCTGTATCTC ACAGAAATAG TGATCAAAGG ATCATCGGAG 
TCTCATTGTG TCCCGCCAGG GAATATCGTC AACTATCTAA ACCTCACACT GTCAAAAGAA 
GGTACTAAAC GAAACAAAAG AGAAGTGGAT TCTGGGTTTC CTCACGCTGA AGCTTCTGTC 
ACCGTCCTGG CGACTCGTAA AGTGGCACAT TTTCTGGACT GTTCAAAGAA GACGGCTCTG 
TGTCAGACCT TCTCCTGCCC GCTGCTCAAC ATGTCCACCC AGGCGGAGGT CAGAGTTCGA 
GCACGGGTGT GGAACGGCAC CTTGCTGGAG GACTATGCCA AAGCCTTGCG AGTGGAAGTA 
AAGGGACAGG CTACATTGAG ACTGCTGACC GATAAACCGG CCATCAAAAT GGACAACCAA 
ACCAGAGAGT TTTCAGTGAA CATTGATCCG GTGTTGGGAG AAGAGACGCC GTATGAAGTT 
CCCCTCTGGA TCATCATTGT TGCAGCGGTT GCAGGAATAC TCTTATTAGG CATCATCAGT 
CTTATTCTCT GGAAGTGCGG TTTCTTCAGA CGGGCCAGCA GGAGAGAGAT GTATGAGGCC 
AAATCCCAGA AAGCAGAAAT AAAGATCCAG CCCTCTGAGA CAGAGAGGCT GACGGAGGAG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001177238.1
CDS	13..3138
Translation	MAGKSLHLCVFVICAIQTNTGFNIDVQFPVIKEGKTKGSLFGFSVALHKQTEKAKKNLLLVGAPQEKAQPQLSKFKINETGAVYYCPITVNLDDCGSMDLISPPKSTDMLEGMWLGVTVASQRNGGRVLACGHRYVKKLLGAEEQQRMVGKCYVRGNDLSYDPSDYWQSETYELCDFNHDQDLEGMCNMGISGGMTEKDVYFGTPGSFTWQGTVHMKCRDTDSDYAGDANDRSFGKLGEDKFNIYIGYSVLEDIKLLDRSVFTVVMGAPRDEFKGSVILADKQDLILKQLMNITGEQIGSYFGSCLAVTDLNNDDWNDLIVGAPFYFDRYKEEGGAVYIFMNENGSFQKKASLVLKGKKGSGFGFAIAAVGDVNQDGFQDIAVGAPFQDSGKVYIWMGSKSGITKEPSQVIEGKSLGPSGFQTFGYSLSGGMDMDGNDYPDILVGSLDDRIALLRSRPVIHLSKNFTVEPKIVIPSQCGNSCIKVKVCFSYTLSNGNSNFRKEITVKFTIDADQNRRGARVRFIDNKQSTFTGLLSFSASSCQELELSVNNPVTDKLQPIVFSLNVSLNQQKAEAQQTLQSLDSFPVLSGQQILTDKAEINFHKECGDDNRCSSNLQLKARFADENYNPFPSQTLEFSSNVKKLVLMVEVSNTADENKPAEDAHQATLNISIPYSLKYSGVRPPAGFDMECSADEAVICDLGNPFKSNQKVSFIIIFETSGITLYTEQIQSQLQLSTISEQADLFPVLVTLNVKNTILTTFSLEKLIIDTSFSGNVIGESAMSSTSDVGSPLEFVFRVQVLGEPLGDLGTLVIDFDWPYAVANGKWLLYLTEIVIKGSSESHCVPPGNIVNYLNLTLSKEGTKRNKREVDSGFPHAEASVTVLATRKVAHFLDCSKKTALCQTFSCPLLNMSTQAEVRVRARVWNGTLLEDYAKALRVEVKGQATLRLLTDKPAIKMDNQTREFSVNIDPVLGEETPYEVPLWIIIVAAVAGILLLGIISLILWKCGFFRRASRREMYEAKSQKAEIKIQPSETERLTEEF

Target ORF information:

RefSeq Version	NM_001190309.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio integrin, alpha 3b (itga3b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001190309.1

ORF Insert Sequence:

ATGGCCGGAA AGTCTCTGCA CTTGTGCGTC TTCGTAATTT GCGCAATTCA AACGAACACC 
GGATTTAACA TTGACGTGCA GTTCCCGGTT ATCAAAGAAG GAAAAACCAA GGGCAGTCTA 
TTTGGATTCT CAGTGGCACT GCACAAACAG ACCGAAAAGG CGAAGAAGAA CTTGCTGTTA 
GTCGGAGCCC CACAAGAAAA AGCCCAGCCT CAACTCAGTA AATTCAAAAT TAATGAAACT 
GGAGCCGTGT ATTATTGTCC CATCACTGTT AACCTGGATG ACTGTGGAAG CATGGACCTC 
ATCAGTCCAC CAAAGTCGAC GGATATGCTG GAGGGAATGT GGCTAGGAGT GACCGTGGCC 
AGTCAGAGGA ATGGAGGCCG TGTGCTGGCA TGTGGTCATC GCTATGTCAA AAAACTGCTG 
GGCGCAGAAG AGCAGCAGCG TATGGTGGGC AAATGTTACG TGCGGGGCAA TGACCTGAGC 
TACGACCCAT CTGATTACTG GCAGTCGGAA ACCTACGAGC TGTGCGACTT CAACCACGAC 
CAGGACCTGG AGGGCATGTG TAACATGGGT ATATCAGGAG GCATGACGGA GAAAGATGTG 
TATTTTGGGA CACCTGGCAG CTTCACGTGG CAGGGCACCG TCCATATGAA GTGCAGAGAC 
ACAGACTCCG ATTACGCTGG CGATGCCAAT GATAGGAGCT TTGGTAAACT GGGTGAGGAC 
AAGTTCAACA TCTACATAGG TTACTCTGTG CTGGAGGACA TAAAGTTACT GGATCGCTCT 
GTGTTCACTG TGGTGATGGG GGCTCCCAGA GATGAATTCA AGGGTTCAGT GATCCTGGCC 
GACAAACAAG ACTTGATACT CAAGCAGCTG ATGAATATCA CAGGGGAACA AATAGGGTCG 
TACTTTGGCA GCTGCTTGGC AGTAACAGAC CTCAACAATG ACGATTGGAA TGACCTGATC 
GTCGGCGCGC CATTTTATTT CGACCGCTAT AAGGAGGAAG GAGGTGCTGT TTACATCTTT 
ATGAACGAAA ACGGATCCTT CCAGAAAAAA GCCAGCCTGG TTCTGAAGGG TAAAAAGGGT 
TCTGGGTTTG GGTTTGCCAT TGCTGCGGTC GGAGATGTCA ATCAGGATGG ATTTCAAGAT 
ATTGCTGTCG GCGCTCCATT CCAGGACTCT GGAAAAGTCT ACATATGGAT GGGAAGTAAA 
AGTGGAATAA CTAAAGAACC CAGCCAGGTG ATCGAGGGTA AGTCATTGGG TCCCAGTGGT 
TTTCAGACGT TTGGTTATTC TCTCAGTGGA GGAATGGATA TGGATGGAAA TGATTATCCA 
GATATTTTGG TGGGCTCTTT GGATGACCGC ATCGCACTCT TGAGGTCTCG TCCGGTCATT 
CATCTCTCCA AAAACTTCAC CGTGGAGCCA AAGATTGTGA TTCCAAGCCA GTGTGGCAAT 
TCCTGCATAA AGGTAAAGGT GTGCTTTAGT TACACTCTCA GCAATGGAAA CAGCAACTTC 
AGGAAGGAAA TCACTGTGAA GTTTACGATA GATGCTGACC AGAATCGCAG AGGTGCTCGA 
GTACGCTTTA TAGACAACAA GCAAAGCACG TTTACAGGCC TTCTGTCTTT CTCTGCTTCT 
TCCTGTCAAG AGCTGGAGCT CAGTGTTAAT AATCCAGTGA CGGACAAACT TCAGCCCATA 
GTGTTCAGTC TGAACGTGTC TCTAAATCAG CAGAAAGCAG AAGCGCAGCA GACATTACAG 
AGTCTAGACT CTTTCCCTGT GCTGAGTGGA CAGCAGATCC TCACCGACAA AGCTGAGATT 
AATTTCCACA AGGAGTGTGG AGATGATAAC CGTTGTAGCA GTAACCTGCA GCTCAAGGCC 
AGGTTTGCAG ATGAAAACTA CAATCCCTTT CCCAGTCAGA CGCTGGAGTT TAGCAGTAAT 
GTGAAGAAGC TGGTGCTGAT GGTGGAGGTC TCCAACACAG CGGATGAGAA CAAACCGGCG 
GAGGATGCTC ATCAGGCCAC GCTCAACATC TCCATTCCAT ACTCACTCAA ATACTCAGGC 
GTCCGTCCTC CGGCTGGTTT TGATATGGAG TGCAGTGCAG ACGAGGCTGT CATATGTGAT 
CTGGGAAACC CATTTAAGAG CAATCAGAAG GTCTCATTTA TAATCATCTT TGAGACCTCT 
GGTATTACAC TTTATACAGA GCAGATCCAA TCTCAGCTGC AGCTCTCCAC TATTAGTGAA 
CAGGCGGATC TGTTTCCTGT TCTTGTCACC CTCAATGTGA AGAACACGAT CCTCACTACA 
TTTTCTTTGG AAAAGCTAAT AATCGACACG TCGTTCAGCG GCAATGTGAT TGGAGAATCT 
GCCATGAGCA GCACCAGTGA TGTGGGGAGT CCATTGGAGT TTGTGTTTCG TGTGCAGGTG 
CTGGGAGAAC CACTGGGAGA TCTGGGAACT CTTGTGATTG ACTTTGACTG GCCGTATGCA 
GTAGCCAATG GGAAATGGCT GCTGTATCTC ACAGAAATAG TGATCAAAGG ATCATCGGAG 
TCTCATTGTG TCCCGCCAGG GAATATCGTC AACTATCTAA ACCTCACACT GTCAAAAGAA 
GGTACTAAAC GAAACAAAAG AGAAGTGGAT TCTGGGTTTC CTCACGCTGA AGCTTCTGTC 
ACCGTCCTGG CGACTCGTAA AGTGGCACAT TTTCTGGACT GTTCAAAGAA GACGGCTCTG 
TGTCAGACCT TCTCCTGCCC GCTGCTCAAC ATGTCCACCC AGGCGGAGGT CAGAGTTCGA 
GCACGGGTGT GGAACGGCAC CTTGCTGGAG GACTATGCCA AAGCCTTGCG AGTGGAAGTA 
AAGGGACAGG CTACATTGAG ACTGCTGACC GATAAACCGG CCATCAAAAT GGACAACCAA 
ACCAGAGAGT TTTCAGTGAA CATTGATCCG GTGTTGGGAG AAGAGACGCC GTATGAAGTT 
CCCCTCTGGA TCATCATTGT TGCAGCGGTT GCAGGAATAC TCTTATTAGG CATCATCAGT 
CTTATTCTCT GGAAGTGCGG TTTCTTCAGA CGGGCCAGCA GGAGAGAGAT GTATGAGGCC 
AAATCCCAGA AAGCAGAAAT AAAGATCCAG CCCTCTGAGA CAGAGAGGCT GACGGAGGAG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itga3b ( XM_009306420.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304695.1 Danio rerio integrin, alpha 3b (itga3b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa49540
Clone ID Related Accession (Same CDS sequence)	XM_009306420.2 , XM_009306420.3
Accession Version	XM_009306420.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	integrin alpha-3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306420.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGAA AGTCTCTGCA CTTGTGCGTC TTCGTAATTT GCGCGATTCA AACGAACACC 
GGATTTAACA TTGACGTGCA GTTCCCGGTT ATCAAAGAAG GAAAAACCAA GGGCAGTCTA 
TTTGGATTCT CAGTGGCACT GCACAAACAG ACCGAAAAGG CGAAGAAGAA CTTGCTGTTA 
GTCGGAGCCC CTCAAGAAAA AGCCCACCCT CAACTCAGTA AATTTAAAAT TAATGAAACT 
GGAGCCGTGT ATTATTGTCC CATCACTGTT AACCAGGATG ACTGCGGAAG CATGGACCTC 
ATCAGTCCAC CAAAGTCGAC GGATATGCTG GAGGGAATGT GGCTAGGAGT GACCGTGGCC 
AGTCAGAGGA ATGGAGGCCG TGTGCTGGCA TGTGGTCATC GCTATGTCAA AAAACTGCTG 
GGCGCAGAAG AGCAGCAGCG TATGGTGGGC AAATGTTACG TGCGGGGCAA TGACCTGAGC 
TACGACCCAT CTGATTACTG GCAGTCGGAA ACCTACGAGC TGTGCGACTT CAACCACGAC 
CAGGACCTGG AGGGCATGTG TAACATGGGT ATATCAGGAG GCATGACGGA GAAAGATGTG 
TATTTTGGGA CACCTGGCAG CTTCACGTGG CAGGGCACCG TCCATATGAA GTGCAGAGAC 
ACAGACTCCG ATTATGCTGG CGATGCCAAT GATAGAAGCT TTGGTAAACT GGGTGAGGAC 
AAGTTCAACA TCTACATAGG TTACTCTGTG CTGGAGGACA TAAAGTTACT GGATCGCTCT 
GTGTTCACTG TGGTGATGGG GGCTCCCAGA GATGAATTCA AGGGTTCAGT GATCCTGGCC 
GACAAACAAG ACTTGATACT CAAGCAGCTG ATGACGATCA CAGGGGAACA AATAGGGTCG 
TACTTTGGCA GCTGCTTGGC AGTAACAGAC CTCAACAATG ACGATTGGAA TGACCTGATC 
GTCGGCGCGC CATTTTATTT CGACCGCTAT AAGGAGGAAG GAGGTGCTGT TTACATTTTT 
ATGAACGAAA ACGGATCCTT CCAGAAAAAA GCCAGCCTGG TTCTGAAGGG TAAAAAGGGA 
TCTGGGTTTG GGTTTGCCGT TGCTGCGGTC GGAGATGTCA ATCAGGACGG ATTTCAAGAT 
ATTGCTGTCG GCGCTCCATT CCAGGACTCT GGAAAAGTCT ACATATGGAT GGGAAGTAAA 
AGTGGAATAA CTAAAGAACC AAGCCAGGTG ATCGAGGGTA AGTCATTGGG TCCCAGTGGT 
TTTCAGACGT TTGGTTATTC TCTCAGTGGA GGAATGGATA TGGATGGAAA TGATTATCCA 
GATATTTTGG TGGGCTCTTT GGATGACCGC ATCGCACTCT TGAGGTCTCG TCCAGTCATT 
CATCTGTCCA AAAACTTCAC CGTGGAGCCA AAGATTGTGA TTCCAAGCCA GTGTGGTATT 
TCCTGCATAA AGGTGAAGGT GTGCTTCAGT TACACTCTCA GCAATGGAAA CAGCAACTTC 
AGGAAGGAAA TCACTGTGAA GTTTACGATA GATGCTGACC AGAATCGCAG AGGTGCTCGA 
GTACGCTTTA TAGACAACAA GCAAAGCACG TTTACAGGCC TTCTGTCTTT CTCTGCTTCT 
TCCTGTCAAG AGCTGGAGCT CAGTGTTAAT AATCCAGTGA CGGACAAACT TCAGCCCATA 
GTGTTCAGTC TGAACGTGTC TCTAAATCAA CAGAAAGCAG CAGCGCAGCA GACATTACAG 
AGTCTAGACT CTTTCCCTGT GCTGAGTGGA CAGCAGATCC TCACCGACAA AGCTGAGATT 
AATTTCCACA AGGAGTGTGG AGATGATAAC CGTTGTAGCA GTAACCTGCA GCTCAAGGCC 
AGGTTTGCAG ATGAAAACTA CAATCCCTTT CCCAGTCAGA CGCTGGAGTT TAGCAGTAAT 
GTGAAGAAGC TGGTGCTGAT GGTGGAGGTC TCCAACACAG CGGATGAGAA CAAACCGGCG 
GAGGATGCTC ATCAGGCCAC GCTCAACATC TCCATTCCAT ACTCACTCAA ATACTCAGGC 
GTCCGTCCTC CGGCTGGTTT TGATATGGAG TGCAGTGCAG ACGAGGCTGT CATATGTGAT 
CTGGGAAACC CATTTAAGAG CAATCAGAAG GTCTCATTTA TAATCATCTT TGAGACCTCT 
GGTATTACAC TTTATACAGA GCAGATCCAA TCTCAGCTGC AGCTCTCCAC TATTAGTGAA 
CAGGCGGATC TGTTTCCTGT TCTTGTCACC CTCAATGTGA AGAACACGAT CCTCACTACA 
TTTTCTTTGG AAAAGCTAAT AATCGACACG TCGTTCAGCG GCAATGTGAT CGGAGAATCT 
GCCATGAGCA GCACCAGTGA TGTGGGGAGT CCATTGGAGT TTGTGTTTCG TGTGCAGGTG 
CTGGGAGAAC CACTGGGAGA TCTGGGAACT CTTGTGATTG ACTTTGACTG GCCGTATGCA 
GTAGCCAATG GGAAATGGCT GCTGTATCTC ACAGAAATAG TGATCAAAGG ATCATCGGAG 
TCTCATTGTG TCCCGCCAGG GAATATCGTC AACTATCTAA ACCTCACACT GTCAAAAGAA 
GGTACTAAAC GAAACAAAAG AGAAGTGGAT TCTGGGTTTC CTCACGCTGA AGCTTCTGTC 
ACCGTCCTGG CGACTCGTAA AGTGGCACAT TTTCTGGACT GTTCAAAGAA GACGGCTCTG 
TGTCAGACCT TCTCCTGCCC GCTGCTCAAC ATGTCCACCC AGGCGGAGGT CAGAGTTCGA 
GCACGGGTGT GGAACGGCAC CTTGCTGGAG GACTATGCCA AAGCCTTGCG AGTGGAAGTA 
AAGGGACAGG CTACATTGAG ATTGCTGACC GATAAACCGG CCATCAAAAT GGACAACCAA 
ACCAGAGAGT TTTCAGTGAA CATTGATCCG GTGTTGGGAG AAGAGACACC GTATGAAGTT 
CCCCTCTGGA TCATCATTGT TGCAGCGGTT GCAGGAATAC TCTTATTAGG CATCATCAGT 
CTTATTCTCT GGAAGTGCGG TTTCTTCAGA CGGGCCAGCA GGAGAGAGAT GTATGAGGCC 
AAATCCCAGA AAGCAGAAAT AAAGATCCAG CCCTCTGAGA CAGAGAGGCT GACGGAGGAG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304695.1
CDS	1..3126
Translation	MAGKSLHLCVFVICAIQTNTGFNIDVQFPVIKEGKTKGSLFGFSVALHKQTEKAKKNLLLVGAPQEKAHPQLSKFKINETGAVYYCPITVNQDDCGSMDLISPPKSTDMLEGMWLGVTVASQRNGGRVLACGHRYVKKLLGAEEQQRMVGKCYVRGNDLSYDPSDYWQSETYELCDFNHDQDLEGMCNMGISGGMTEKDVYFGTPGSFTWQGTVHMKCRDTDSDYAGDANDRSFGKLGEDKFNIYIGYSVLEDIKLLDRSVFTVVMGAPRDEFKGSVILADKQDLILKQLMTITGEQIGSYFGSCLAVTDLNNDDWNDLIVGAPFYFDRYKEEGGAVYIFMNENGSFQKKASLVLKGKKGSGFGFAVAAVGDVNQDGFQDIAVGAPFQDSGKVYIWMGSKSGITKEPSQVIEGKSLGPSGFQTFGYSLSGGMDMDGNDYPDILVGSLDDRIALLRSRPVIHLSKNFTVEPKIVIPSQCGISCIKVKVCFSYTLSNGNSNFRKEITVKFTIDADQNRRGARVRFIDNKQSTFTGLLSFSASSCQELELSVNNPVTDKLQPIVFSLNVSLNQQKAAAQQTLQSLDSFPVLSGQQILTDKAEINFHKECGDDNRCSSNLQLKARFADENYNPFPSQTLEFSSNVKKLVLMVEVSNTADENKPAEDAHQATLNISIPYSLKYSGVRPPAGFDMECSADEAVICDLGNPFKSNQKVSFIIIFETSGITLYTEQIQSQLQLSTISEQADLFPVLVTLNVKNTILTTFSLEKLIIDTSFSGNVIGESAMSSTSDVGSPLEFVFRVQVLGEPLGDLGTLVIDFDWPYAVANGKWLLYLTEIVIKGSSESHCVPPGNIVNYLNLTLSKEGTKRNKREVDSGFPHAEASVTVLATRKVAHFLDCSKKTALCQTFSCPLLNMSTQAEVRVRARVWNGTLLEDYAKALRVEVKGQATLRLLTDKPAIKMDNQTREFSVNIDPVLGEETPYEVPLWIIIVAAVAGILLLGIISLILWKCGFFRRASRREMYEAKSQKAEIKIQPSETERLTEEF

Target ORF information:

RefSeq Version	XM_009306420.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio integrin, alpha 3b (itga3b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306420.2

ORF Insert Sequence:

ATGGCCGGAA AGTCTCTGCA CTTGTGCGTC TTCGTAATTT GCGCGATTCA AACGAACACC 
GGATTTAACA TTGACGTGCA GTTCCCGGTT ATCAAAGAAG GAAAAACCAA GGGCAGTCTA 
TTTGGATTCT CAGTGGCACT GCACAAACAG ACCGAAAAGG CGAAGAAGAA CTTGCTGTTA 
GTCGGAGCCC CTCAAGAAAA AGCCCACCCT CAACTCAGTA AATTTAAAAT TAATGAAACT 
GGAGCCGTGT ATTATTGTCC CATCACTGTT AACCAGGATG ACTGCGGAAG CATGGACCTC 
ATCAGTCCAC CAAAGTCGAC GGATATGCTG GAGGGAATGT GGCTAGGAGT GACCGTGGCC 
AGTCAGAGGA ATGGAGGCCG TGTGCTGGCA TGTGGTCATC GCTATGTCAA AAAACTGCTG 
GGCGCAGAAG AGCAGCAGCG TATGGTGGGC AAATGTTACG TGCGGGGCAA TGACCTGAGC 
TACGACCCAT CTGATTACTG GCAGTCGGAA ACCTACGAGC TGTGCGACTT CAACCACGAC 
CAGGACCTGG AGGGCATGTG TAACATGGGT ATATCAGGAG GCATGACGGA GAAAGATGTG 
TATTTTGGGA CACCTGGCAG CTTCACGTGG CAGGGCACCG TCCATATGAA GTGCAGAGAC 
ACAGACTCCG ATTATGCTGG CGATGCCAAT GATAGAAGCT TTGGTAAACT GGGTGAGGAC 
AAGTTCAACA TCTACATAGG TTACTCTGTG CTGGAGGACA TAAAGTTACT GGATCGCTCT 
GTGTTCACTG TGGTGATGGG GGCTCCCAGA GATGAATTCA AGGGTTCAGT GATCCTGGCC 
GACAAACAAG ACTTGATACT CAAGCAGCTG ATGACGATCA CAGGGGAACA AATAGGGTCG 
TACTTTGGCA GCTGCTTGGC AGTAACAGAC CTCAACAATG ACGATTGGAA TGACCTGATC 
GTCGGCGCGC CATTTTATTT CGACCGCTAT AAGGAGGAAG GAGGTGCTGT TTACATTTTT 
ATGAACGAAA ACGGATCCTT CCAGAAAAAA GCCAGCCTGG TTCTGAAGGG TAAAAAGGGA 
TCTGGGTTTG GGTTTGCCGT TGCTGCGGTC GGAGATGTCA ATCAGGACGG ATTTCAAGAT 
ATTGCTGTCG GCGCTCCATT CCAGGACTCT GGAAAAGTCT ACATATGGAT GGGAAGTAAA 
AGTGGAATAA CTAAAGAACC AAGCCAGGTG ATCGAGGGTA AGTCATTGGG TCCCAGTGGT 
TTTCAGACGT TTGGTTATTC TCTCAGTGGA GGAATGGATA TGGATGGAAA TGATTATCCA 
GATATTTTGG TGGGCTCTTT GGATGACCGC ATCGCACTCT TGAGGTCTCG TCCAGTCATT 
CATCTGTCCA AAAACTTCAC CGTGGAGCCA AAGATTGTGA TTCCAAGCCA GTGTGGTATT 
TCCTGCATAA AGGTGAAGGT GTGCTTCAGT TACACTCTCA GCAATGGAAA CAGCAACTTC 
AGGAAGGAAA TCACTGTGAA GTTTACGATA GATGCTGACC AGAATCGCAG AGGTGCTCGA 
GTACGCTTTA TAGACAACAA GCAAAGCACG TTTACAGGCC TTCTGTCTTT CTCTGCTTCT 
TCCTGTCAAG AGCTGGAGCT CAGTGTTAAT AATCCAGTGA CGGACAAACT TCAGCCCATA 
GTGTTCAGTC TGAACGTGTC TCTAAATCAA CAGAAAGCAG CAGCGCAGCA GACATTACAG 
AGTCTAGACT CTTTCCCTGT GCTGAGTGGA CAGCAGATCC TCACCGACAA AGCTGAGATT 
AATTTCCACA AGGAGTGTGG AGATGATAAC CGTTGTAGCA GTAACCTGCA GCTCAAGGCC 
AGGTTTGCAG ATGAAAACTA CAATCCCTTT CCCAGTCAGA CGCTGGAGTT TAGCAGTAAT 
GTGAAGAAGC TGGTGCTGAT GGTGGAGGTC TCCAACACAG CGGATGAGAA CAAACCGGCG 
GAGGATGCTC ATCAGGCCAC GCTCAACATC TCCATTCCAT ACTCACTCAA ATACTCAGGC 
GTCCGTCCTC CGGCTGGTTT TGATATGGAG TGCAGTGCAG ACGAGGCTGT CATATGTGAT 
CTGGGAAACC CATTTAAGAG CAATCAGAAG GTCTCATTTA TAATCATCTT TGAGACCTCT 
GGTATTACAC TTTATACAGA GCAGATCCAA TCTCAGCTGC AGCTCTCCAC TATTAGTGAA 
CAGGCGGATC TGTTTCCTGT TCTTGTCACC CTCAATGTGA AGAACACGAT CCTCACTACA 
TTTTCTTTGG AAAAGCTAAT AATCGACACG TCGTTCAGCG GCAATGTGAT CGGAGAATCT 
GCCATGAGCA GCACCAGTGA TGTGGGGAGT CCATTGGAGT TTGTGTTTCG TGTGCAGGTG 
CTGGGAGAAC CACTGGGAGA TCTGGGAACT CTTGTGATTG ACTTTGACTG GCCGTATGCA 
GTAGCCAATG GGAAATGGCT GCTGTATCTC ACAGAAATAG TGATCAAAGG ATCATCGGAG 
TCTCATTGTG TCCCGCCAGG GAATATCGTC AACTATCTAA ACCTCACACT GTCAAAAGAA 
GGTACTAAAC GAAACAAAAG AGAAGTGGAT TCTGGGTTTC CTCACGCTGA AGCTTCTGTC 
ACCGTCCTGG CGACTCGTAA AGTGGCACAT TTTCTGGACT GTTCAAAGAA GACGGCTCTG 
TGTCAGACCT TCTCCTGCCC GCTGCTCAAC ATGTCCACCC AGGCGGAGGT CAGAGTTCGA 
GCACGGGTGT GGAACGGCAC CTTGCTGGAG GACTATGCCA AAGCCTTGCG AGTGGAAGTA 
AAGGGACAGG CTACATTGAG ATTGCTGACC GATAAACCGG CCATCAAAAT GGACAACCAA 
ACCAGAGAGT TTTCAGTGAA CATTGATCCG GTGTTGGGAG AAGAGACACC GTATGAAGTT 
CCCCTCTGGA TCATCATTGT TGCAGCGGTT GCAGGAATAC TCTTATTAGG CATCATCAGT 
CTTATTCTCT GGAAGTGCGG TTTCTTCAGA CGGGCCAGCA GGAGAGAGAT GTATGAGGCC 
AAATCCCAGA AAGCAGAAAT AAAGATCCAG CCCTCTGAGA CAGAGAGGCT GACGGAGGAG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itga3b ( XM_009306420.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304695.1 Danio rerio integrin, alpha 3b (itga3b), transcript variant X1, mRNA.

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CloneID	ODa49540
Clone ID Related Accession (Same CDS sequence)	XM_009306420.2 , XM_009306420.3
Accession Version	XM_009306420.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	integrin alpha-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306420.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGAA AGTCTCTGCA CTTGTGCGTC TTCGTAATTT GCGCGATTCA AACGAACACC 
GGATTTAACA TTGACGTGCA GTTCCCGGTT ATCAAAGAAG GAAAAACCAA GGGCAGTCTA 
TTTGGATTCT CAGTGGCACT GCACAAACAG ACCGAAAAGG CGAAGAAGAA CTTGCTGTTA 
GTCGGAGCCC CTCAAGAAAA AGCCCACCCT CAACTCAGTA AATTTAAAAT TAATGAAACT 
GGAGCCGTGT ATTATTGTCC CATCACTGTT AACCAGGATG ACTGCGGAAG CATGGACCTC 
ATCAGTCCAC CAAAGTCGAC GGATATGCTG GAGGGAATGT GGCTAGGAGT GACCGTGGCC 
AGTCAGAGGA ATGGAGGCCG TGTGCTGGCA TGTGGTCATC GCTATGTCAA AAAACTGCTG 
GGCGCAGAAG AGCAGCAGCG TATGGTGGGC AAATGTTACG TGCGGGGCAA TGACCTGAGC 
TACGACCCAT CTGATTACTG GCAGTCGGAA ACCTACGAGC TGTGCGACTT CAACCACGAC 
CAGGACCTGG AGGGCATGTG TAACATGGGT ATATCAGGAG GCATGACGGA GAAAGATGTG 
TATTTTGGGA CACCTGGCAG CTTCACGTGG CAGGGCACCG TCCATATGAA GTGCAGAGAC 
ACAGACTCCG ATTATGCTGG CGATGCCAAT GATAGAAGCT TTGGTAAACT GGGTGAGGAC 
AAGTTCAACA TCTACATAGG TTACTCTGTG CTGGAGGACA TAAAGTTACT GGATCGCTCT 
GTGTTCACTG TGGTGATGGG GGCTCCCAGA GATGAATTCA AGGGTTCAGT GATCCTGGCC 
GACAAACAAG ACTTGATACT CAAGCAGCTG ATGACGATCA CAGGGGAACA AATAGGGTCG 
TACTTTGGCA GCTGCTTGGC AGTAACAGAC CTCAACAATG ACGATTGGAA TGACCTGATC 
GTCGGCGCGC CATTTTATTT CGACCGCTAT AAGGAGGAAG GAGGTGCTGT TTACATTTTT 
ATGAACGAAA ACGGATCCTT CCAGAAAAAA GCCAGCCTGG TTCTGAAGGG TAAAAAGGGA 
TCTGGGTTTG GGTTTGCCGT TGCTGCGGTC GGAGATGTCA ATCAGGACGG ATTTCAAGAT 
ATTGCTGTCG GCGCTCCATT CCAGGACTCT GGAAAAGTCT ACATATGGAT GGGAAGTAAA 
AGTGGAATAA CTAAAGAACC AAGCCAGGTG ATCGAGGGTA AGTCATTGGG TCCCAGTGGT 
TTTCAGACGT TTGGTTATTC TCTCAGTGGA GGAATGGATA TGGATGGAAA TGATTATCCA 
GATATTTTGG TGGGCTCTTT GGATGACCGC ATCGCACTCT TGAGGTCTCG TCCAGTCATT 
CATCTGTCCA AAAACTTCAC CGTGGAGCCA AAGATTGTGA TTCCAAGCCA GTGTGGTATT 
TCCTGCATAA AGGTGAAGGT GTGCTTCAGT TACACTCTCA GCAATGGAAA CAGCAACTTC 
AGGAAGGAAA TCACTGTGAA GTTTACGATA GATGCTGACC AGAATCGCAG AGGTGCTCGA 
GTACGCTTTA TAGACAACAA GCAAAGCACG TTTACAGGCC TTCTGTCTTT CTCTGCTTCT 
TCCTGTCAAG AGCTGGAGCT CAGTGTTAAT AATCCAGTGA CGGACAAACT TCAGCCCATA 
GTGTTCAGTC TGAACGTGTC TCTAAATCAA CAGAAAGCAG CAGCGCAGCA GACATTACAG 
AGTCTAGACT CTTTCCCTGT GCTGAGTGGA CAGCAGATCC TCACCGACAA AGCTGAGATT 
AATTTCCACA AGGAGTGTGG AGATGATAAC CGTTGTAGCA GTAACCTGCA GCTCAAGGCC 
AGGTTTGCAG ATGAAAACTA CAATCCCTTT CCCAGTCAGA CGCTGGAGTT TAGCAGTAAT 
GTGAAGAAGC TGGTGCTGAT GGTGGAGGTC TCCAACACAG CGGATGAGAA CAAACCGGCG 
GAGGATGCTC ATCAGGCCAC GCTCAACATC TCCATTCCAT ACTCACTCAA ATACTCAGGC 
GTCCGTCCTC CGGCTGGTTT TGATATGGAG TGCAGTGCAG ACGAGGCTGT CATATGTGAT 
CTGGGAAACC CATTTAAGAG CAATCAGAAG GTCTCATTTA TAATCATCTT TGAGACCTCT 
GGTATTACAC TTTATACAGA GCAGATCCAA TCTCAGCTGC AGCTCTCCAC TATTAGTGAA 
CAGGCGGATC TGTTTCCTGT TCTTGTCACC CTCAATGTGA AGAACACGAT CCTCACTACA 
TTTTCTTTGG AAAAGCTAAT AATCGACACG TCGTTCAGCG GCAATGTGAT CGGAGAATCT 
GCCATGAGCA GCACCAGTGA TGTGGGGAGT CCATTGGAGT TTGTGTTTCG TGTGCAGGTG 
CTGGGAGAAC CACTGGGAGA TCTGGGAACT CTTGTGATTG ACTTTGACTG GCCGTATGCA 
GTAGCCAATG GGAAATGGCT GCTGTATCTC ACAGAAATAG TGATCAAAGG ATCATCGGAG 
TCTCATTGTG TCCCGCCAGG GAATATCGTC AACTATCTAA ACCTCACACT GTCAAAAGAA 
GGTACTAAAC GAAACAAAAG AGAAGTGGAT TCTGGGTTTC CTCACGCTGA AGCTTCTGTC 
ACCGTCCTGG CGACTCGTAA AGTGGCACAT TTTCTGGACT GTTCAAAGAA GACGGCTCTG 
TGTCAGACCT TCTCCTGCCC GCTGCTCAAC ATGTCCACCC AGGCGGAGGT CAGAGTTCGA 
GCACGGGTGT GGAACGGCAC CTTGCTGGAG GACTATGCCA AAGCCTTGCG AGTGGAAGTA 
AAGGGACAGG CTACATTGAG ATTGCTGACC GATAAACCGG CCATCAAAAT GGACAACCAA 
ACCAGAGAGT TTTCAGTGAA CATTGATCCG GTGTTGGGAG AAGAGACACC GTATGAAGTT 
CCCCTCTGGA TCATCATTGT TGCAGCGGTT GCAGGAATAC TCTTATTAGG CATCATCAGT 
CTTATTCTCT GGAAGTGCGG TTTCTTCAGA CGGGCCAGCA GGAGAGAGAT GTATGAGGCC 
AAATCCCAGA AAGCAGAAAT AAAGATCCAG CCCTCTGAGA CAGAGAGGCT GACGGAGGAG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304695.1
CDS	219..3344
Translation	MAGKSLHLCVFVICAIQTNTGFNIDVQFPVIKEGKTKGSLFGFSVALHKQTEKAKKNLLLVGAPQEKAHPQLSKFKINETGAVYYCPITVNQDDCGSMDLISPPKSTDMLEGMWLGVTVASQRNGGRVLACGHRYVKKLLGAEEQQRMVGKCYVRGNDLSYDPSDYWQSETYELCDFNHDQDLEGMCNMGISGGMTEKDVYFGTPGSFTWQGTVHMKCRDTDSDYAGDANDRSFGKLGEDKFNIYIGYSVLEDIKLLDRSVFTVVMGAPRDEFKGSVILADKQDLILKQLMTITGEQIGSYFGSCLAVTDLNNDDWNDLIVGAPFYFDRYKEEGGAVYIFMNENGSFQKKASLVLKGKKGSGFGFAVAAVGDVNQDGFQDIAVGAPFQDSGKVYIWMGSKSGITKEPSQVIEGKSLGPSGFQTFGYSLSGGMDMDGNDYPDILVGSLDDRIALLRSRPVIHLSKNFTVEPKIVIPSQCGISCIKVKVCFSYTLSNGNSNFRKEITVKFTIDADQNRRGARVRFIDNKQSTFTGLLSFSASSCQELELSVNNPVTDKLQPIVFSLNVSLNQQKAAAQQTLQSLDSFPVLSGQQILTDKAEINFHKECGDDNRCSSNLQLKARFADENYNPFPSQTLEFSSNVKKLVLMVEVSNTADENKPAEDAHQATLNISIPYSLKYSGVRPPAGFDMECSADEAVICDLGNPFKSNQKVSFIIIFETSGITLYTEQIQSQLQLSTISEQADLFPVLVTLNVKNTILTTFSLEKLIIDTSFSGNVIGESAMSSTSDVGSPLEFVFRVQVLGEPLGDLGTLVIDFDWPYAVANGKWLLYLTEIVIKGSSESHCVPPGNIVNYLNLTLSKEGTKRNKREVDSGFPHAEASVTVLATRKVAHFLDCSKKTALCQTFSCPLLNMSTQAEVRVRARVWNGTLLEDYAKALRVEVKGQATLRLLTDKPAIKMDNQTREFSVNIDPVLGEETPYEVPLWIIIVAAVAGILLLGIISLILWKCGFFRRASRREMYEAKSQKAEIKIQPSETERLTEEF

Target ORF information:

RefSeq Version	XM_009306420.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio integrin, alpha 3b (itga3b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306420.3

ORF Insert Sequence:

ATGGCCGGAA AGTCTCTGCA CTTGTGCGTC TTCGTAATTT GCGCGATTCA AACGAACACC 
GGATTTAACA TTGACGTGCA GTTCCCGGTT ATCAAAGAAG GAAAAACCAA GGGCAGTCTA 
TTTGGATTCT CAGTGGCACT GCACAAACAG ACCGAAAAGG CGAAGAAGAA CTTGCTGTTA 
GTCGGAGCCC CTCAAGAAAA AGCCCACCCT CAACTCAGTA AATTTAAAAT TAATGAAACT 
GGAGCCGTGT ATTATTGTCC CATCACTGTT AACCAGGATG ACTGCGGAAG CATGGACCTC 
ATCAGTCCAC CAAAGTCGAC GGATATGCTG GAGGGAATGT GGCTAGGAGT GACCGTGGCC 
AGTCAGAGGA ATGGAGGCCG TGTGCTGGCA TGTGGTCATC GCTATGTCAA AAAACTGCTG 
GGCGCAGAAG AGCAGCAGCG TATGGTGGGC AAATGTTACG TGCGGGGCAA TGACCTGAGC 
TACGACCCAT CTGATTACTG GCAGTCGGAA ACCTACGAGC TGTGCGACTT CAACCACGAC 
CAGGACCTGG AGGGCATGTG TAACATGGGT ATATCAGGAG GCATGACGGA GAAAGATGTG 
TATTTTGGGA CACCTGGCAG CTTCACGTGG CAGGGCACCG TCCATATGAA GTGCAGAGAC 
ACAGACTCCG ATTATGCTGG CGATGCCAAT GATAGAAGCT TTGGTAAACT GGGTGAGGAC 
AAGTTCAACA TCTACATAGG TTACTCTGTG CTGGAGGACA TAAAGTTACT GGATCGCTCT 
GTGTTCACTG TGGTGATGGG GGCTCCCAGA GATGAATTCA AGGGTTCAGT GATCCTGGCC 
GACAAACAAG ACTTGATACT CAAGCAGCTG ATGACGATCA CAGGGGAACA AATAGGGTCG 
TACTTTGGCA GCTGCTTGGC AGTAACAGAC CTCAACAATG ACGATTGGAA TGACCTGATC 
GTCGGCGCGC CATTTTATTT CGACCGCTAT AAGGAGGAAG GAGGTGCTGT TTACATTTTT 
ATGAACGAAA ACGGATCCTT CCAGAAAAAA GCCAGCCTGG TTCTGAAGGG TAAAAAGGGA 
TCTGGGTTTG GGTTTGCCGT TGCTGCGGTC GGAGATGTCA ATCAGGACGG ATTTCAAGAT 
ATTGCTGTCG GCGCTCCATT CCAGGACTCT GGAAAAGTCT ACATATGGAT GGGAAGTAAA 
AGTGGAATAA CTAAAGAACC AAGCCAGGTG ATCGAGGGTA AGTCATTGGG TCCCAGTGGT 
TTTCAGACGT TTGGTTATTC TCTCAGTGGA GGAATGGATA TGGATGGAAA TGATTATCCA 
GATATTTTGG TGGGCTCTTT GGATGACCGC ATCGCACTCT TGAGGTCTCG TCCAGTCATT 
CATCTGTCCA AAAACTTCAC CGTGGAGCCA AAGATTGTGA TTCCAAGCCA GTGTGGTATT 
TCCTGCATAA AGGTGAAGGT GTGCTTCAGT TACACTCTCA GCAATGGAAA CAGCAACTTC 
AGGAAGGAAA TCACTGTGAA GTTTACGATA GATGCTGACC AGAATCGCAG AGGTGCTCGA 
GTACGCTTTA TAGACAACAA GCAAAGCACG TTTACAGGCC TTCTGTCTTT CTCTGCTTCT 
TCCTGTCAAG AGCTGGAGCT CAGTGTTAAT AATCCAGTGA CGGACAAACT TCAGCCCATA 
GTGTTCAGTC TGAACGTGTC TCTAAATCAA CAGAAAGCAG CAGCGCAGCA GACATTACAG 
AGTCTAGACT CTTTCCCTGT GCTGAGTGGA CAGCAGATCC TCACCGACAA AGCTGAGATT 
AATTTCCACA AGGAGTGTGG AGATGATAAC CGTTGTAGCA GTAACCTGCA GCTCAAGGCC 
AGGTTTGCAG ATGAAAACTA CAATCCCTTT CCCAGTCAGA CGCTGGAGTT TAGCAGTAAT 
GTGAAGAAGC TGGTGCTGAT GGTGGAGGTC TCCAACACAG CGGATGAGAA CAAACCGGCG 
GAGGATGCTC ATCAGGCCAC GCTCAACATC TCCATTCCAT ACTCACTCAA ATACTCAGGC 
GTCCGTCCTC CGGCTGGTTT TGATATGGAG TGCAGTGCAG ACGAGGCTGT CATATGTGAT 
CTGGGAAACC CATTTAAGAG CAATCAGAAG GTCTCATTTA TAATCATCTT TGAGACCTCT 
GGTATTACAC TTTATACAGA GCAGATCCAA TCTCAGCTGC AGCTCTCCAC TATTAGTGAA 
CAGGCGGATC TGTTTCCTGT TCTTGTCACC CTCAATGTGA AGAACACGAT CCTCACTACA 
TTTTCTTTGG AAAAGCTAAT AATCGACACG TCGTTCAGCG GCAATGTGAT CGGAGAATCT 
GCCATGAGCA GCACCAGTGA TGTGGGGAGT CCATTGGAGT TTGTGTTTCG TGTGCAGGTG 
CTGGGAGAAC CACTGGGAGA TCTGGGAACT CTTGTGATTG ACTTTGACTG GCCGTATGCA 
GTAGCCAATG GGAAATGGCT GCTGTATCTC ACAGAAATAG TGATCAAAGG ATCATCGGAG 
TCTCATTGTG TCCCGCCAGG GAATATCGTC AACTATCTAA ACCTCACACT GTCAAAAGAA 
GGTACTAAAC GAAACAAAAG AGAAGTGGAT TCTGGGTTTC CTCACGCTGA AGCTTCTGTC 
ACCGTCCTGG CGACTCGTAA AGTGGCACAT TTTCTGGACT GTTCAAAGAA GACGGCTCTG 
TGTCAGACCT TCTCCTGCCC GCTGCTCAAC ATGTCCACCC AGGCGGAGGT CAGAGTTCGA 
GCACGGGTGT GGAACGGCAC CTTGCTGGAG GACTATGCCA AAGCCTTGCG AGTGGAAGTA 
AAGGGACAGG CTACATTGAG ATTGCTGACC GATAAACCGG CCATCAAAAT GGACAACCAA 
ACCAGAGAGT TTTCAGTGAA CATTGATCCG GTGTTGGGAG AAGAGACACC GTATGAAGTT 
CCCCTCTGGA TCATCATTGT TGCAGCGGTT GCAGGAATAC TCTTATTAGG CATCATCAGT 
CTTATTCTCT GGAAGTGCGG TTTCTTCAGA CGGGCCAGCA GGAGAGAGAT GTATGAGGCC 
AAATCCCAGA AAGCAGAAAT AAAGATCCAG CCCTCTGAGA CAGAGAGGCT GACGGAGGAG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Focal adhesions are essential to drive zebrafish heart valve morphogenesis.
The Journal of cell biology218(3)1039-1054(2019 03)
Gunawan F,Gentile A,Fukuda R,Tsedeke AT,Jiménez-Amilburu V,Ramadass R,Iida A,Sehara-Fujisawa A,Stainier DYR

Deficiency of lrp4 in zebrafish and human LRP4 mutation induce aberrant activation of Jagged-Notch signaling in fin and limb development.
Cellular and molecular life sciences : CMLS76(1)163-178(2019 Jan)
Tian J,Shao J,Liu C,Hou HY,Chou CW,Shboul M,Li GQ,El-Khateeb M,Samarah OQ,Kou Y,Chen YH,Chen MJ,Lyu Z,Chen WL,Chen YF,Sun YH,Liu YW

Gon4l regulates notochord boundary formation and cell polarity underlying axis extension by repressing adhesion genes.
Nature communications9(1)1319(2018 04)
Williams MLK,Sawada A,Budine T,Yin C,Gontarz P,Solnica-Krezel L

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Zebrafish Collagen Type I: Molecular and Biochemical Characterization of the Major Structural Protein in Bone and Skin.
Scientific reports621540(2016 Feb)
Gistelinck C,Gioia R,Gagliardi A,Tonelli F,Marchese L,Bianchi L,Landi C,Bini L,Huysseune A,Witten PE,Staes A,Gevaert K,De Rocker N,Menten B,Malfait F,Leikin S,Carra S,Tenni R,Rossi A,De Paepe A,Coucke P,Willaert A,Forlino A

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itga3b cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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