myot cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	myot
Entrez Gene ID	100329862
Full Name	myotilin
Synonyms	fi31g01,si:ch211-194m19.4,wu:fi31g01
General protein information	Preferred Names myotilin Names myotilin
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009307788.2	XP_009306063.1	myotilin isoform X2
XM_002664328.5	XP_002664374.4	myotilin isoform X1
XM_009307788.3	XP_009306063.1	myotilin isoform X3
XM_002664328.6	XP_002664374.4	myotilin isoform X1
XM_021481128.1	XP_021336803.1	myotilin isoform X2
XM_021472760.1	XP_021328435.1	myotilin isoform X4

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MYOT	NP_001068803.1
Rattus norvegicus (Norway rat)	Myot	NP_001099618.1
Rattus norvegicus (Norway rat)	LOC100909761	XP_006255053.1
Pan troglodytes (chimpanzee)	MYOT	XP_517948.2
Xenopus tropicalis (tropical clawed frog)	myot	NP_001017159.2
Canis lupus familiaris (dog)	MYOT	XP_003639423.1
Rattus norvegicus (Norway rat)	LOC100912306	XP_006244741.1
Homo sapiens (human)	MYOT	NP_006781.1
Mus musculus (house mouse)	Myot	NP_001028793.1
Macaca mulatta (Rhesus monkey)	MYOT	XP_001106921.2
Gallus gallus (chicken)	MYOT	XP_414618.4
Danio rerio (zebrafish)	LOC100329862	XP_002664374.3

Gene Ontology

The following myot gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the myot cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa145593	XM_021472760.1 Latest version!	Danio rerio myotilin (myot), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODa148800	XM_021481128.1 Latest version!	Danio rerio myotilin (myot), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa49254	XM_002664328.6 Latest version!	Danio rerio myotilin (myot), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa49255	XM_009307788.3 Latest version!	Danio rerio myotilin (myot), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
ODa49255	XM_009307788.2	Danio rerio myotilin (myot), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODa49254	XM_002664328.5	Danio rerio myotilin (myot), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

myot ( XM_021472760.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328435.1 Danio rerio myotilin (myot), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa145593
Clone ID Related Accession (Same CDS sequence)	XM_021472760.1
Accession Version	XM_021472760.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	myotilin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394896.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGAAG TATATACAGA CCTAGAAGAG GTGCTGGGAG ATGAGCAAGA CCCCTCCTCT 
ATACAATCAG ATGTACTAGT AAAAGATGAC CATTGCTTAG TACTACAAGA TACATGTGCC 
GCTTCTCCAG AATGGCCTAA AAGTTCAGAG GAAGAAACTG CAACCCCTAT GTCATGGGTA 
CTGGAGTCTG GCATTAAAAA GGTTGAGTCT CTCCCAGACA CTGACAGCGA ACAGAGTTCT 
GTCAGTAAGG TGGCTGAAGA TACAAACCTC TCCCCAATGA TCAAACTTGA TTCTCCACCA 
AAGGGAGTTA AAAACAGTGT TCCACATCCA TTCCTTCTCA CCAGCAAGCT GAATAAACAA 
GTAGAACCCA AGGGCAGTGC CACGAATGTG GCTGACCTGC CCACCTTTTC ACCATCATTG 
TACCCTCCAA GTGCTTTTAA TTATGAGCGC CCTCGCCATT TTATCCAGTC CCTGCCCAGT 
TTCCACACAC ATGACACTGA GACCAGCAAG CCAAATGATC TTTGTGTTCC AGCTACAACT 
CCTGCACCCT TAGTGACCCC TACTCTTCAT GCACCCACTC AGACATTAAT GTGTTCCAGC 
ATGACTTTGA TTCCTAAAGC AAGGAGTACT CCAAACAGTG AGAATCTATC ACCAGCAGCT 
TTCCTATCAT CAGTCCTGCC TTCACCTCCA AGCTGCCAGT CAAAATGCCT TTCCATACCT 
CAATCACCCA GGCACTCCTC TAGATCACCC AGCCCAACAC AAAAGGTCCA AGAACCACCC 
CTATCTCCTT CAAATCAGTT ACGGAATCTC CCTGCCCGGA TACCTCCAGA TACATTGAAA 
ACTACAGCTC GATCTCTGCA GTCTTCACCT GTGCCCCATA TTACATATAC ATCCAACATG 
TTGCCAAAAC CAATCTTGAA GAAGAATGTT TCACGACCAG TGTCTCGTGC CACAGATGAA 
GACATTCAGG GCTCTAAAGA TGCTCTTATG CAAGACCTTG AACAGAAGCT ACGAAACAAA 
GTTCCCCAAC AAAGAAACAA TCAGCAGAAG ATTTCTTATG AGGAACGGAT GGCCAGAAGA 
TTGCTGGGTG CTGATAATGC AGCTTCAGTT TTTCATCTCG AAAATTCCTT CAGTTCACAG 
CCTGCACAGC CAGGGTCTCC AGAAGATCAG TACCCTGGAG GAATATGGTC AAGAAAAAAA 
CGATCAGGGG GGGACAGTAC AGAAGCCTCT ACCATCCAAG AGAAATGTTT TGCTCCTCGT 
TTTATACAAG TGCCTCAAGA TGTTACTGTT GAAGAGGGAC GATTCTGCAG GATTGATTTT 
AAGGTTGTAG GTTTGCCAAC CCCAGATATA TCTTGGTATC TGGATGGGAA ACCCATTCGA 
CCTGATGACT ATCATAAGAT GCTGGTGTGT GAGAAAAGTG TCCACTCCTT CATTATTGAG 
ATTGTAACCA TTCATCATGC GGGTTTATAT GAATGTATTG CAAAGAACCG TGCTGGACAG 
AGCCAATTCT CCCTACATTT GGATGTGATT GCTCAGGAAC AATTATGTCC TCCTACATTT 
GTAGTGAAGA TGAAGAACTC ACATGCAATG GAGGGTGATG CTGTTAGGTT AGAATGTAAA 
GTGAATGCAT CTCCAAGCCC TCAGCTATAC TGGAAGAAAG ACAAAGAAAT GCTGCGTATT 
GACCATACGA GAATGAGTCT TTCTCAGGAC AGTTCAGGAA AGCAGTGCCT GGTCATTAAC 
CCAGTGATGA AGTCTGATGC TGGCTGGTAT ACTGTATCTG CTATTAATGA AGCAGGAATG 
TCCACGTGCA ATGCCAGGCT TGACGTAGCC ACGCGATTGA ACAAGACAAC CCATCCTGCT 
AGGCCACTCA AAAACCTTCC CATCGTAAAC CAGTTTTCCA CACTGTGCCT TGACCCTCTT 
GTTCAGCACA CTGCCCCTCT TTATGAAAGT GAAGAACTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328435.1
CDS	120..2081
Translation	MLEVYTDLEEVLGDEQDPSSIQSDVLVKDDHCLVLQDTCAASPEWPKSSEEETATPMSWVLESGIKKVESLPDTDSEQSSVSKVAEDTNLSPMIKLDSPPKGVKNSVPHPFLLTSKLNKQVEPKGSATNVADLPTFSPSLYPPSAFNYERPRHFIQSLPSFHTHDTETSKPNDLCVPATTPAPLVTPTLHAPTQTLMCSSMTLIPKARSTPNSENLSPAAFLSSVLPSPPSCQSKCLSIPQSPRHSSRSPSPTQKVQEPPLSPSNQLRNLPARIPPDTLKTTARSLQSSPVPHITYTSNMLPKPILKKNVSRPVSRATDEDIQGSKDALMQDLEQKLRNKVPQQRNNQQKISYEERMARRLLGADNAASVFHLENSFSSQPAQPGSPEDQYPGGIWSRKKRSGGDSTEASTIQEKCFAPRFIQVPQDVTVEEGRFCRIDFKVVGLPTPDISWYLDGKPIRPDDYHKMLVCEKSVHSFIIEIVTIHHAGLYECIAKNRAGQSQFSLHLDVIAQEQLCPPTFVVKMKNSHAMEGDAVRLECKVNASPSPQLYWKKDKEMLRIDHTRMSLSQDSSGKQCLVINPVMKSDAGWYTVSAINEAGMSTCNARLDVATRLNKTTHPARPLKNLPIVNQFSTLCLDPLVQHTAPLYESEEL

Target ORF information:

RefSeq Version	XM_021472760.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myotilin (myot), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472760.1

ORF Insert Sequence:

ATGCTGGAAG TATATACAGA CCTAGAAGAG GTGCTGGGAG ATGAGCAAGA CCCCTCCTCT 
ATACAATCAG ATGTACTAGT AAAAGATGAC CATTGCTTAG TACTACAAGA TACATGTGCC 
GCTTCTCCAG AATGGCCTAA AAGTTCAGAG GAAGAAACTG CAACCCCTAT GTCATGGGTA 
CTGGAGTCTG GCATTAAAAA GGTTGAGTCT CTCCCAGACA CTGACAGCGA ACAGAGTTCT 
GTCAGTAAGG TGGCTGAAGA TACAAACCTC TCCCCAATGA TCAAACTTGA TTCTCCACCA 
AAGGGAGTTA AAAACAGTGT TCCACATCCA TTCCTTCTCA CCAGCAAGCT GAATAAACAA 
GTAGAACCCA AGGGCAGTGC CACGAATGTG GCTGACCTGC CCACCTTTTC ACCATCATTG 
TACCCTCCAA GTGCTTTTAA TTATGAGCGC CCTCGCCATT TTATCCAGTC CCTGCCCAGT 
TTCCACACAC ATGACACTGA GACCAGCAAG CCAAATGATC TTTGTGTTCC AGCTACAACT 
CCTGCACCCT TAGTGACCCC TACTCTTCAT GCACCCACTC AGACATTAAT GTGTTCCAGC 
ATGACTTTGA TTCCTAAAGC AAGGAGTACT CCAAACAGTG AGAATCTATC ACCAGCAGCT 
TTCCTATCAT CAGTCCTGCC TTCACCTCCA AGCTGCCAGT CAAAATGCCT TTCCATACCT 
CAATCACCCA GGCACTCCTC TAGATCACCC AGCCCAACAC AAAAGGTCCA AGAACCACCC 
CTATCTCCTT CAAATCAGTT ACGGAATCTC CCTGCCCGGA TACCTCCAGA TACATTGAAA 
ACTACAGCTC GATCTCTGCA GTCTTCACCT GTGCCCCATA TTACATATAC ATCCAACATG 
TTGCCAAAAC CAATCTTGAA GAAGAATGTT TCACGACCAG TGTCTCGTGC CACAGATGAA 
GACATTCAGG GCTCTAAAGA TGCTCTTATG CAAGACCTTG AACAGAAGCT ACGAAACAAA 
GTTCCCCAAC AAAGAAACAA TCAGCAGAAG ATTTCTTATG AGGAACGGAT GGCCAGAAGA 
TTGCTGGGTG CTGATAATGC AGCTTCAGTT TTTCATCTCG AAAATTCCTT CAGTTCACAG 
CCTGCACAGC CAGGGTCTCC AGAAGATCAG TACCCTGGAG GAATATGGTC AAGAAAAAAA 
CGATCAGGGG GGGACAGTAC AGAAGCCTCT ACCATCCAAG AGAAATGTTT TGCTCCTCGT 
TTTATACAAG TGCCTCAAGA TGTTACTGTT GAAGAGGGAC GATTCTGCAG GATTGATTTT 
AAGGTTGTAG GTTTGCCAAC CCCAGATATA TCTTGGTATC TGGATGGGAA ACCCATTCGA 
CCTGATGACT ATCATAAGAT GCTGGTGTGT GAGAAAAGTG TCCACTCCTT CATTATTGAG 
ATTGTAACCA TTCATCATGC GGGTTTATAT GAATGTATTG CAAAGAACCG TGCTGGACAG 
AGCCAATTCT CCCTACATTT GGATGTGATT GCTCAGGAAC AATTATGTCC TCCTACATTT 
GTAGTGAAGA TGAAGAACTC ACATGCAATG GAGGGTGATG CTGTTAGGTT AGAATGTAAA 
GTGAATGCAT CTCCAAGCCC TCAGCTATAC TGGAAGAAAG ACAAAGAAAT GCTGCGTATT 
GACCATACGA GAATGAGTCT TTCTCAGGAC AGTTCAGGAA AGCAGTGCCT GGTCATTAAC 
CCAGTGATGA AGTCTGATGC TGGCTGGTAT ACTGTATCTG CTATTAATGA AGCAGGAATG 
TCCACGTGCA ATGCCAGGCT TGACGTAGCC ACGCGATTGA ACAAGACAAC CCATCCTGCT 
AGGCCACTCA AAAACCTTCC CATCGTAAAC CAGTTTTCCA CACTGTGCCT TGACCCTCTT 
GTTCAGCACA CTGCCCCTCT TTATGAAAGT GAAGAACTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myot ( XM_021481128.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336803.1 Danio rerio myotilin (myot), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa148800
Clone ID Related Accession (Same CDS sequence)	XM_021481128.1
Accession Version	XM_021481128.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2442bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	myotilin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGG TTGAGAAAAA AACATCAACT ATGTCCGTAA CTGTAACATC ATGCTCCTGT 
GAAACAACCT CCTTGTCTTC AACATCATCT TCCTCTTCTT CAGTGCTTCA GCAAAAACAC 
CCCCATTTAG TACAGCCTCT CTGCATCAGT CCACAATTGG TTCAGAACAA TAACCAGGGT 
TCAGGAAATG TGGGAGTTTC TCCAACATAT GTCAAGTGTC TCCATGATGT CAGCACATTG 
AAGGGTCAGT TAGTGGTTCT GGAGTGCAGG GTCAGAGGAA CTCCTCCACT GCAGATCTCC 
TGGTATAGAG AAGACAAGCA AATCATTGAC TCTGCAGACT TACGCATTCT TAGAAAAAAG 
GCAAGTTCAG CTTTTATTCC GGAGGAATTG TGCACCCTAG TGATCACAGA GGCATGTCCA 
GAAGATTCTG GAATATTTAA ATGCATTGCA TCAAATCAGT TTGGAACAGT AACATGCAGT 
GCCATGCTGG AAGTATATAC AGACCTAGAA GAGGTGCTGG GAGATGAGCA AGACCCCTCC 
TCTATACAAT CAGATGTACT AGTAAAAGAT GACCATTGCT TAGTACTACA AGATACATGT 
GCCGCTTCTC CAGAATGGCC TAAAAGTTCA GAGGAAGAAA CTGCAACCCC TATGTCATGG 
GTACTGGAGT CTGGCATTAA AAAGGTTGAG TCTCTCCCAG ACACTGACAG CGAACAGAGT 
TCTGTCAGTA AGGTGGCTGA AGATACAAAC CTCTCCCCAA TGATCAAACT TGATTCTCCA 
CCAAAGGGAG TTAAAAACAG TGTTCCACAT CCATTCCTTC TCACCAGCAA GCTGAATAAA 
CAAGTAGAAT CCAAGGGCAG TGCCACGAAT GTGGCTGACC TGCCCACCTT TTCACCATCA 
TTGTACCCTC CAAGTGCTTT TAATTATGAG CGCCCTCGCC ATTTTATCCA GTCCCTGCCC 
AGTTTCCACA CACATGACAC TGAGACCAGC AAGCCAAATG ATCTTTGTGT TCCAGCTACA 
ACTCCTGCAC CCTTAGTGAC CCCTCCTCTT CATGCACCCA CTCAGACATT AATGTGTTCC 
AGCATGACTT TGATTCCTAA AGCAAGGAGT ACTCCAAACA GTGAGAATCT ATCACCAGCA 
GCTTTCCTAT CATCAGTCCT GCCTTCACCT CCAAGCTGCC AGTCAAAATG CCTTTCCATA 
CCTCAATCAC CCAGGCACTC CTCTAGATCA CCCAGCCCAA CACAAAAGGT CCAAGAACCA 
CCCCTATCTC CTTCAAATCA GTTACGGAAT CTCCCTGCCC GGATACCTCC AGATACATTG 
AAAACTACAG CTCGATCTCT GCAGTCTTCA CCTGTGCCCC ATATTACATA TACATCCAAC 
ATGTTGCCAA AACCAATCTT GAAGAAGAAT GTTTCACGAC CAGTGTCTCG TGCCACAGAT 
GAAGACATTC AGGGCTCTAA AGATGCTCTT ATGCAAGACC TTGAACAGAA GCTACGAAAC 
AAAGTTCCCC AACAAAGAAA CAATCAGAAG ATTTCTTATG AGGAACGGAT GGCCAGAAGA 
TTGCTGGGTG CTGATAATGC AGCTTCAGTT TTTCATCTCG AAAATTCCTT CAGTTCACAG 
CCTGCACAGC CAGGGTCTCC AGAAGATCAG TACCCTGGAG GAATATGGTC AAGAAAAAAA 
CGATCAGGGG GGGACAGTAC AGAAGCCTCT ACCATCCAAG AGAAATGTTT TGCTCCTCGT 
TTTATACAAG TGCCTCAAGA TGTTACTGTT GAAGAGGGAC GATTCTGCAG GATTGATTTT 
AAGGTTGTAG GTTTGCCAAC CCCAGATATA TCTTGGTATC TGGATGGGAA ACCCATTCGA 
CCTGATGACT ATCATAAGAT GCTGGTGTGT GAGAAAAGTG TCCACTCCTT CATTATTGAG 
ATTGTAACCA TTCATCATGC GGGTTTATAT GAATGTATTG CAAAGAACCG TGCTGGACAG 
AGCCAATTCT CCCTACATTT GGATGTGATT GCTCAGGAAC AATTATGTCC TCCTACATTT 
GTAGTGAAGA TGAAGAACTC ACATGCAATG GAGGGTGATG CTGTTAGGTT AGAATGTAAA 
GTGAATGCAT CTCCAAGCCC TCAGCTATAC TGGAAGAAAG ACAAAGAAAT GCTGCGTATT 
GACCATACGA GAATGAGTCT TTCTCAGGAC AGTTCAGGAA AGCAGTGCCT GGTCATTAAC 
CCAGTGATGA AGTCTGATGC TGGCTGGTAT ACTGTTTCCG CTATTAATGA AGCAGGAATG 
TCCACGTGCA ATGCCAGGCT TGACGTAGCC ACGCGATTGA ACAAGACAAC CCATCCTGCT 
AGGCCACTCA AAAACCTTCC CATCGTAAAC CAGTTTTCCA CACTGTGCCT TGACCCTCTT 
GTTCAGCACA CTGCCCCTCT TTATGAAAGT GAAGAACTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336803.1
CDS	283..2724
Translation	MAQVEKKTSTMSVTVTSCSCETTSLSSTSSSSSSVLQQKHPHLVQPLCISPQLVQNNNQGSGNVGVSPTYVKCLHDVSTLKGQLVVLECRVRGTPPLQISWYREDKQIIDSADLRILRKKASSAFIPEELCTLVITEACPEDSGIFKCIASNQFGTVTCSAMLEVYTDLEEVLGDEQDPSSIQSDVLVKDDHCLVLQDTCAASPEWPKSSEEETATPMSWVLESGIKKVESLPDTDSEQSSVSKVAEDTNLSPMIKLDSPPKGVKNSVPHPFLLTSKLNKQVESKGSATNVADLPTFSPSLYPPSAFNYERPRHFIQSLPSFHTHDTETSKPNDLCVPATTPAPLVTPPLHAPTQTLMCSSMTLIPKARSTPNSENLSPAAFLSSVLPSPPSCQSKCLSIPQSPRHSSRSPSPTQKVQEPPLSPSNQLRNLPARIPPDTLKTTARSLQSSPVPHITYTSNMLPKPILKKNVSRPVSRATDEDIQGSKDALMQDLEQKLRNKVPQQRNNQKISYEERMARRLLGADNAASVFHLENSFSSQPAQPGSPEDQYPGGIWSRKKRSGGDSTEASTIQEKCFAPRFIQVPQDVTVEEGRFCRIDFKVVGLPTPDISWYLDGKPIRPDDYHKMLVCEKSVHSFIIEIVTIHHAGLYECIAKNRAGQSQFSLHLDVIAQEQLCPPTFVVKMKNSHAMEGDAVRLECKVNASPSPQLYWKKDKEMLRIDHTRMSLSQDSSGKQCLVINPVMKSDAGWYTVSAINEAGMSTCNARLDVATRLNKTTHPARPLKNLPIVNQFSTLCLDPLVQHTAPLYESEEL

Target ORF information:

RefSeq Version	XM_021481128.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myotilin (myot), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481128.1

ORF Insert Sequence:

ATGGCACAGG TTGAGAAAAA AACATCAACT ATGTCCGTAA CTGTAACATC ATGCTCCTGT 
GAAACAACCT CCTTGTCTTC AACATCATCT TCCTCTTCTT CAGTGCTTCA GCAAAAACAC 
CCCCATTTAG TACAGCCTCT CTGCATCAGT CCACAATTGG TTCAGAACAA TAACCAGGGT 
TCAGGAAATG TGGGAGTTTC TCCAACATAT GTCAAGTGTC TCCATGATGT CAGCACATTG 
AAGGGTCAGT TAGTGGTTCT GGAGTGCAGG GTCAGAGGAA CTCCTCCACT GCAGATCTCC 
TGGTATAGAG AAGACAAGCA AATCATTGAC TCTGCAGACT TACGCATTCT TAGAAAAAAG 
GCAAGTTCAG CTTTTATTCC GGAGGAATTG TGCACCCTAG TGATCACAGA GGCATGTCCA 
GAAGATTCTG GAATATTTAA ATGCATTGCA TCAAATCAGT TTGGAACAGT AACATGCAGT 
GCCATGCTGG AAGTATATAC AGACCTAGAA GAGGTGCTGG GAGATGAGCA AGACCCCTCC 
TCTATACAAT CAGATGTACT AGTAAAAGAT GACCATTGCT TAGTACTACA AGATACATGT 
GCCGCTTCTC CAGAATGGCC TAAAAGTTCA GAGGAAGAAA CTGCAACCCC TATGTCATGG 
GTACTGGAGT CTGGCATTAA AAAGGTTGAG TCTCTCCCAG ACACTGACAG CGAACAGAGT 
TCTGTCAGTA AGGTGGCTGA AGATACAAAC CTCTCCCCAA TGATCAAACT TGATTCTCCA 
CCAAAGGGAG TTAAAAACAG TGTTCCACAT CCATTCCTTC TCACCAGCAA GCTGAATAAA 
CAAGTAGAAT CCAAGGGCAG TGCCACGAAT GTGGCTGACC TGCCCACCTT TTCACCATCA 
TTGTACCCTC CAAGTGCTTT TAATTATGAG CGCCCTCGCC ATTTTATCCA GTCCCTGCCC 
AGTTTCCACA CACATGACAC TGAGACCAGC AAGCCAAATG ATCTTTGTGT TCCAGCTACA 
ACTCCTGCAC CCTTAGTGAC CCCTCCTCTT CATGCACCCA CTCAGACATT AATGTGTTCC 
AGCATGACTT TGATTCCTAA AGCAAGGAGT ACTCCAAACA GTGAGAATCT ATCACCAGCA 
GCTTTCCTAT CATCAGTCCT GCCTTCACCT CCAAGCTGCC AGTCAAAATG CCTTTCCATA 
CCTCAATCAC CCAGGCACTC CTCTAGATCA CCCAGCCCAA CACAAAAGGT CCAAGAACCA 
CCCCTATCTC CTTCAAATCA GTTACGGAAT CTCCCTGCCC GGATACCTCC AGATACATTG 
AAAACTACAG CTCGATCTCT GCAGTCTTCA CCTGTGCCCC ATATTACATA TACATCCAAC 
ATGTTGCCAA AACCAATCTT GAAGAAGAAT GTTTCACGAC CAGTGTCTCG TGCCACAGAT 
GAAGACATTC AGGGCTCTAA AGATGCTCTT ATGCAAGACC TTGAACAGAA GCTACGAAAC 
AAAGTTCCCC AACAAAGAAA CAATCAGAAG ATTTCTTATG AGGAACGGAT GGCCAGAAGA 
TTGCTGGGTG CTGATAATGC AGCTTCAGTT TTTCATCTCG AAAATTCCTT CAGTTCACAG 
CCTGCACAGC CAGGGTCTCC AGAAGATCAG TACCCTGGAG GAATATGGTC AAGAAAAAAA 
CGATCAGGGG GGGACAGTAC AGAAGCCTCT ACCATCCAAG AGAAATGTTT TGCTCCTCGT 
TTTATACAAG TGCCTCAAGA TGTTACTGTT GAAGAGGGAC GATTCTGCAG GATTGATTTT 
AAGGTTGTAG GTTTGCCAAC CCCAGATATA TCTTGGTATC TGGATGGGAA ACCCATTCGA 
CCTGATGACT ATCATAAGAT GCTGGTGTGT GAGAAAAGTG TCCACTCCTT CATTATTGAG 
ATTGTAACCA TTCATCATGC GGGTTTATAT GAATGTATTG CAAAGAACCG TGCTGGACAG 
AGCCAATTCT CCCTACATTT GGATGTGATT GCTCAGGAAC AATTATGTCC TCCTACATTT 
GTAGTGAAGA TGAAGAACTC ACATGCAATG GAGGGTGATG CTGTTAGGTT AGAATGTAAA 
GTGAATGCAT CTCCAAGCCC TCAGCTATAC TGGAAGAAAG ACAAAGAAAT GCTGCGTATT 
GACCATACGA GAATGAGTCT TTCTCAGGAC AGTTCAGGAA AGCAGTGCCT GGTCATTAAC 
CCAGTGATGA AGTCTGATGC TGGCTGGTAT ACTGTTTCCG CTATTAATGA AGCAGGAATG 
TCCACGTGCA ATGCCAGGCT TGACGTAGCC ACGCGATTGA ACAAGACAAC CCATCCTGCT 
AGGCCACTCA AAAACCTTCC CATCGTAAAC CAGTTTTCCA CACTGTGCCT TGACCCTCTT 
GTTCAGCACA CTGCCCCTCT TTATGAAAGT GAAGAACTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myot ( XM_002664328.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_002664374.4 Danio rerio myotilin (myot), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa49254
Clone ID Related Accession (Same CDS sequence)	XM_002664328.5 , XM_002664328.6
Accession Version	XM_002664328.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2445bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	myotilin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_002664328.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGG TTGAGAAAAA AACATCAACT ATGTCCGTAA CTGTAACATC ATGCTCCTGT 
GAAACAACCT CCTTGTCTTC AACATCATCT TCCTCTTCTT CAGTGCTTCA GCAAAAACAC 
CCCCATTTAG TACAGCCTCT CTGCATCAGT CCACAATTGG TTCAGAACAA TAACCAGGGT 
TCAGGAAATG TGGGAGTTTC TCCAACATAT GTCAAGTGTC TCCATGATGT CAGCACATTG 
AAGGGTCAGT TAGTGGTTCT GGAGTGCAGG GTCAGAGGAA CTCCTCCACT GCAGATCTCC 
TGGTATAGAG AAGACAAGCA AATCATTGAC TCTGCAGACT TACGCATTCT TAGAAAAAAG 
GCAAGTTCAG CTTTTATTCC GGAGGAATTG TGCACCCTAG TGATCACAGA GGCATGTCCA 
GAAGATTCTG GAATATTTAA ATGCATTGCA TCAAATCAGT TTGGAACAGT AACATGCAGT 
GCCATGCTGG AAGTATATAC AGACCTAGAA GAGGTGCTGG GAGATGAGCA AGACCCCTCC 
TCTATACAAT CAGATGTACT AGTAAAAGAT GACCATTGCT TAGTACTACA AGATACATGT 
GCCGCTTCTC CAGAATGGCC TAAAAGTTCA GAGGAAGAAA CTGCAACCCC TATGTCATGG 
GTACTGGAGT CTGGCATTAA AAAGGTTGAG TCTCTCCCAG ACACTGACAG CGAACAGAGT 
TCTGTCAGTA AGGTGGCTGA AGATACAAAC CTCTCCCCAA TGATCAAACT TGATTCTCCA 
CCAAAGGGAG TTAAAAACAG TGTTCCACAT CCATTCCTTC TCACCAGCAA GCTGAATAAA 
CAAGTAGAAT CCAAGGGCAG TGCCACGAAT GTGGCTGACC TGCCCACCTT TTCACCATCA 
TTGTACCCTC CAAGTGCTTT TAATTATGAG CGCCCTCGCC ATTTTATCCA GTCCCTGCCC 
AGTTTCCACA CACATGACAC TGAGACCAGC AAGCCAAATG ATCTTTGTGT TCCAGCTACA 
ACTCCTGCAC CCTTAGTGAC CCCTCCTCTT CATGCACCCA CTCAGACATT AATGTGTTCC 
AGCATGACTT TGATTCCTAA AGCAAGGAGT ACTCCAAACA GTGAGAATCT ATCACCAGCA 
GCTTTCCTAT CATCAGTCCT GCCTTCACCT CCAAGCTGCC AGTCAAAATG CCTTTCCATA 
CCTCAATCAC CCAGGCACTC CTCTAGATCA CCCAGCCCAA CACAAAAGGT CCAAGAACCA 
CCCCTATCTC CTTCAAATCA GTTACGGAAT CTCCCTGCCC GGATACCTCC AGATACATTG 
AAAACTACAG CTCGATCTCT GCAGTCTTCA CCTGTGCCCC ATATTACATA TACATCCAAC 
ATGTTGCCAA AACCAATCTT GAAGAAGAAT GTTTCACGAC CAGTGTCTCG TGCCACAGAT 
GAAGACATTC AGGGCTCTAA AGATGCTCTT ATGCAAGACC TTGAACAGAA GCTACGAAAC 
AAAGTTCCCC AACAAAGAAA CAATCAGCAG AAGATTTCTT ATGAGGAACG GATGGCCAGA 
AGATTGCTGG GTGCTGATAA TGCAGCTTCA GTTTTTCATC TCGAAAATTC CTTCAGTTCA 
CAGCCTGCAC AGCCAGGGTC TCCAGAAGAT CAGTACCCTG GAGGAATATG GTCAAGAAAA 
AAACGATCAG GGGGGGACAG TACAGAAGCC TCTACCATCC AAGAGAAATG TTTTGCTCCT 
CGTTTTATAC AAGTGCCTCA AGATGTTACT GTTGAAGAGG GACGATTCTG CAGGATTGAT 
TTTAAGGTTG TAGGTTTGCC AACCCCAGAT ATATCTTGGT ATCTGGATGG GAAACCCATT 
CGACCTGATG ACTATCATAA GATGCTGGTG TGTGAGAAAA GTGTCCACTC CTTCATTATT 
GAGATTGTAA CCATTCATCA TGCGGGTTTA TATGAATGTA TTGCAAAGAA CCGTGCTGGA 
CAGAGCCAAT TCTCCCTACA TTTGGATGTG ATTGCTCAGG AACAATTATG TCCTCCTACA 
TTTGTAGTGA AGATGAAGAA CTCACATGCA ATGGAGGGTG ATGCTGTTAG GTTAGAATGT 
AAAGTGAATG CATCTCCAAG CCCTCAGCTA TACTGGAAGA AAGACAAAGA AATGCTGCGT 
ATTGACCATA CGAGAATGAG TCTTTCTCAG GACAGTTCAG GAAAGCAGTG CCTGGTCATT 
AACCCAGTGA TGAAGTCTGA TGCTGGCTGG TATACTGTTT CCGCTATTAA TGAAGCAGGA 
ATGTCCACGT GCAATGCCAG GCTTGACGTA GCCACGCGAT TGAACAAGAC AACCCATCCT 
GCTAGGCCAC TCAAAAACCT TCCCATCGTA AACCAGTTTT CCACACTGTG CCTTGACCCT 
CTTGTTCAGC ACACTGCCCC TCTTTATGAA AGTGAAGAAC TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002664374.4
CDS	258..2702
Translation	MAQVEKKTSTMSVTVTSCSCETTSLSSTSSSSSSVLQQKHPHLVQPLCISPQLVQNNNQGSGNVGVSPTYVKCLHDVSTLKGQLVVLECRVRGTPPLQISWYREDKQIIDSADLRILRKKASSAFIPEELCTLVITEACPEDSGIFKCIASNQFGTVTCSAMLEVYTDLEEVLGDEQDPSSIQSDVLVKDDHCLVLQDTCAASPEWPKSSEEETATPMSWVLESGIKKVESLPDTDSEQSSVSKVAEDTNLSPMIKLDSPPKGVKNSVPHPFLLTSKLNKQVESKGSATNVADLPTFSPSLYPPSAFNYERPRHFIQSLPSFHTHDTETSKPNDLCVPATTPAPLVTPPLHAPTQTLMCSSMTLIPKARSTPNSENLSPAAFLSSVLPSPPSCQSKCLSIPQSPRHSSRSPSPTQKVQEPPLSPSNQLRNLPARIPPDTLKTTARSLQSSPVPHITYTSNMLPKPILKKNVSRPVSRATDEDIQGSKDALMQDLEQKLRNKVPQQRNNQQKISYEERMARRLLGADNAASVFHLENSFSSQPAQPGSPEDQYPGGIWSRKKRSGGDSTEASTIQEKCFAPRFIQVPQDVTVEEGRFCRIDFKVVGLPTPDISWYLDGKPIRPDDYHKMLVCEKSVHSFIIEIVTIHHAGLYECIAKNRAGQSQFSLHLDVIAQEQLCPPTFVVKMKNSHAMEGDAVRLECKVNASPSPQLYWKKDKEMLRIDHTRMSLSQDSSGKQCLVINPVMKSDAGWYTVSAINEAGMSTCNARLDVATRLNKTTHPARPLKNLPIVNQFSTLCLDPLVQHTAPLYESEEL

Target ORF information:

RefSeq Version	XM_002664328.5
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio myotilin (myot), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002664328.5

ORF Insert Sequence:

ATGGCACAGG TTGAGAAAAA AACATCAACT ATGTCCGTAA CTGTAACATC ATGCTCCTGT 
GAAACAACCT CCTTGTCTTC AACATCATCT TCCTCTTCTT CAGTGCTTCA GCAAAAACAC 
CCCCATTTAG TACAGCCTCT CTGCATCAGT CCACAATTGG TTCAGAACAA TAACCAGGGT 
TCAGGAAATG TGGGAGTTTC TCCAACATAT GTCAAGTGTC TCCATGATGT CAGCACATTG 
AAGGGTCAGT TAGTGGTTCT GGAGTGCAGG GTCAGAGGAA CTCCTCCACT GCAGATCTCC 
TGGTATAGAG AAGACAAGCA AATCATTGAC TCTGCAGACT TACGCATTCT TAGAAAAAAG 
GCAAGTTCAG CTTTTATTCC GGAGGAATTG TGCACCCTAG TGATCACAGA GGCATGTCCA 
GAAGATTCTG GAATATTTAA ATGCATTGCA TCAAATCAGT TTGGAACAGT AACATGCAGT 
GCCATGCTGG AAGTATATAC AGACCTAGAA GAGGTGCTGG GAGATGAGCA AGACCCCTCC 
TCTATACAAT CAGATGTACT AGTAAAAGAT GACCATTGCT TAGTACTACA AGATACATGT 
GCCGCTTCTC CAGAATGGCC TAAAAGTTCA GAGGAAGAAA CTGCAACCCC TATGTCATGG 
GTACTGGAGT CTGGCATTAA AAAGGTTGAG TCTCTCCCAG ACACTGACAG CGAACAGAGT 
TCTGTCAGTA AGGTGGCTGA AGATACAAAC CTCTCCCCAA TGATCAAACT TGATTCTCCA 
CCAAAGGGAG TTAAAAACAG TGTTCCACAT CCATTCCTTC TCACCAGCAA GCTGAATAAA 
CAAGTAGAAT CCAAGGGCAG TGCCACGAAT GTGGCTGACC TGCCCACCTT TTCACCATCA 
TTGTACCCTC CAAGTGCTTT TAATTATGAG CGCCCTCGCC ATTTTATCCA GTCCCTGCCC 
AGTTTCCACA CACATGACAC TGAGACCAGC AAGCCAAATG ATCTTTGTGT TCCAGCTACA 
ACTCCTGCAC CCTTAGTGAC CCCTCCTCTT CATGCACCCA CTCAGACATT AATGTGTTCC 
AGCATGACTT TGATTCCTAA AGCAAGGAGT ACTCCAAACA GTGAGAATCT ATCACCAGCA 
GCTTTCCTAT CATCAGTCCT GCCTTCACCT CCAAGCTGCC AGTCAAAATG CCTTTCCATA 
CCTCAATCAC CCAGGCACTC CTCTAGATCA CCCAGCCCAA CACAAAAGGT CCAAGAACCA 
CCCCTATCTC CTTCAAATCA GTTACGGAAT CTCCCTGCCC GGATACCTCC AGATACATTG 
AAAACTACAG CTCGATCTCT GCAGTCTTCA CCTGTGCCCC ATATTACATA TACATCCAAC 
ATGTTGCCAA AACCAATCTT GAAGAAGAAT GTTTCACGAC CAGTGTCTCG TGCCACAGAT 
GAAGACATTC AGGGCTCTAA AGATGCTCTT ATGCAAGACC TTGAACAGAA GCTACGAAAC 
AAAGTTCCCC AACAAAGAAA CAATCAGCAG AAGATTTCTT ATGAGGAACG GATGGCCAGA 
AGATTGCTGG GTGCTGATAA TGCAGCTTCA GTTTTTCATC TCGAAAATTC CTTCAGTTCA 
CAGCCTGCAC AGCCAGGGTC TCCAGAAGAT CAGTACCCTG GAGGAATATG GTCAAGAAAA 
AAACGATCAG GGGGGGACAG TACAGAAGCC TCTACCATCC AAGAGAAATG TTTTGCTCCT 
CGTTTTATAC AAGTGCCTCA AGATGTTACT GTTGAAGAGG GACGATTCTG CAGGATTGAT 
TTTAAGGTTG TAGGTTTGCC AACCCCAGAT ATATCTTGGT ATCTGGATGG GAAACCCATT 
CGACCTGATG ACTATCATAA GATGCTGGTG TGTGAGAAAA GTGTCCACTC CTTCATTATT 
GAGATTGTAA CCATTCATCA TGCGGGTTTA TATGAATGTA TTGCAAAGAA CCGTGCTGGA 
CAGAGCCAAT TCTCCCTACA TTTGGATGTG ATTGCTCAGG AACAATTATG TCCTCCTACA 
TTTGTAGTGA AGATGAAGAA CTCACATGCA ATGGAGGGTG ATGCTGTTAG GTTAGAATGT 
AAAGTGAATG CATCTCCAAG CCCTCAGCTA TACTGGAAGA AAGACAAAGA AATGCTGCGT 
ATTGACCATA CGAGAATGAG TCTTTCTCAG GACAGTTCAG GAAAGCAGTG CCTGGTCATT 
AACCCAGTGA TGAAGTCTGA TGCTGGCTGG TATACTGTTT CCGCTATTAA TGAAGCAGGA 
ATGTCCACGT GCAATGCCAG GCTTGACGTA GCCACGCGAT TGAACAAGAC AACCCATCCT 
GCTAGGCCAC TCAAAAACCT TCCCATCGTA AACCAGTTTT CCACACTGTG CCTTGACCCT 
CTTGTTCAGC ACACTGCCCC TCTTTATGAA AGTGAAGAAC TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myot ( XM_002664328.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_002664374.4 Danio rerio myotilin (myot), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa49254
Clone ID Related Accession (Same CDS sequence)	XM_002664328.5 , XM_002664328.6
Accession Version	XM_002664328.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2445bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	myotilin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_002664328.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGG TTGAGAAAAA AACATCAACT ATGTCCGTAA CTGTAACATC ATGCTCCTGT 
GAAACAACCT CCTTGTCTTC AACATCATCT TCCTCTTCTT CAGTGCTTCA GCAAAAACAC 
CCCCATTTAG TACAGCCTCT CTGCATCAGT CCACAATTGG TTCAGAACAA TAACCAGGGT 
TCAGGAAATG TGGGAGTTTC TCCAACATAT GTCAAGTGTC TCCATGATGT CAGCACATTG 
AAGGGTCAGT TAGTGGTTCT GGAGTGCAGG GTCAGAGGAA CTCCTCCACT GCAGATCTCC 
TGGTATAGAG AAGACAAGCA AATCATTGAC TCTGCAGACT TACGCATTCT TAGAAAAAAG 
GCAAGTTCAG CTTTTATTCC GGAGGAATTG TGCACCCTAG TGATCACAGA GGCATGTCCA 
GAAGATTCTG GAATATTTAA ATGCATTGCA TCAAATCAGT TTGGAACAGT AACATGCAGT 
GCCATGCTGG AAGTATATAC AGACCTAGAA GAGGTGCTGG GAGATGAGCA AGACCCCTCC 
TCTATACAAT CAGATGTACT AGTAAAAGAT GACCATTGCT TAGTACTACA AGATACATGT 
GCCGCTTCTC CAGAATGGCC TAAAAGTTCA GAGGAAGAAA CTGCAACCCC TATGTCATGG 
GTACTGGAGT CTGGCATTAA AAAGGTTGAG TCTCTCCCAG ACACTGACAG CGAACAGAGT 
TCTGTCAGTA AGGTGGCTGA AGATACAAAC CTCTCCCCAA TGATCAAACT TGATTCTCCA 
CCAAAGGGAG TTAAAAACAG TGTTCCACAT CCATTCCTTC TCACCAGCAA GCTGAATAAA 
CAAGTAGAAT CCAAGGGCAG TGCCACGAAT GTGGCTGACC TGCCCACCTT TTCACCATCA 
TTGTACCCTC CAAGTGCTTT TAATTATGAG CGCCCTCGCC ATTTTATCCA GTCCCTGCCC 
AGTTTCCACA CACATGACAC TGAGACCAGC AAGCCAAATG ATCTTTGTGT TCCAGCTACA 
ACTCCTGCAC CCTTAGTGAC CCCTCCTCTT CATGCACCCA CTCAGACATT AATGTGTTCC 
AGCATGACTT TGATTCCTAA AGCAAGGAGT ACTCCAAACA GTGAGAATCT ATCACCAGCA 
GCTTTCCTAT CATCAGTCCT GCCTTCACCT CCAAGCTGCC AGTCAAAATG CCTTTCCATA 
CCTCAATCAC CCAGGCACTC CTCTAGATCA CCCAGCCCAA CACAAAAGGT CCAAGAACCA 
CCCCTATCTC CTTCAAATCA GTTACGGAAT CTCCCTGCCC GGATACCTCC AGATACATTG 
AAAACTACAG CTCGATCTCT GCAGTCTTCA CCTGTGCCCC ATATTACATA TACATCCAAC 
ATGTTGCCAA AACCAATCTT GAAGAAGAAT GTTTCACGAC CAGTGTCTCG TGCCACAGAT 
GAAGACATTC AGGGCTCTAA AGATGCTCTT ATGCAAGACC TTGAACAGAA GCTACGAAAC 
AAAGTTCCCC AACAAAGAAA CAATCAGCAG AAGATTTCTT ATGAGGAACG GATGGCCAGA 
AGATTGCTGG GTGCTGATAA TGCAGCTTCA GTTTTTCATC TCGAAAATTC CTTCAGTTCA 
CAGCCTGCAC AGCCAGGGTC TCCAGAAGAT CAGTACCCTG GAGGAATATG GTCAAGAAAA 
AAACGATCAG GGGGGGACAG TACAGAAGCC TCTACCATCC AAGAGAAATG TTTTGCTCCT 
CGTTTTATAC AAGTGCCTCA AGATGTTACT GTTGAAGAGG GACGATTCTG CAGGATTGAT 
TTTAAGGTTG TAGGTTTGCC AACCCCAGAT ATATCTTGGT ATCTGGATGG GAAACCCATT 
CGACCTGATG ACTATCATAA GATGCTGGTG TGTGAGAAAA GTGTCCACTC CTTCATTATT 
GAGATTGTAA CCATTCATCA TGCGGGTTTA TATGAATGTA TTGCAAAGAA CCGTGCTGGA 
CAGAGCCAAT TCTCCCTACA TTTGGATGTG ATTGCTCAGG AACAATTATG TCCTCCTACA 
TTTGTAGTGA AGATGAAGAA CTCACATGCA ATGGAGGGTG ATGCTGTTAG GTTAGAATGT 
AAAGTGAATG CATCTCCAAG CCCTCAGCTA TACTGGAAGA AAGACAAAGA AATGCTGCGT 
ATTGACCATA CGAGAATGAG TCTTTCTCAG GACAGTTCAG GAAAGCAGTG CCTGGTCATT 
AACCCAGTGA TGAAGTCTGA TGCTGGCTGG TATACTGTTT CCGCTATTAA TGAAGCAGGA 
ATGTCCACGT GCAATGCCAG GCTTGACGTA GCCACGCGAT TGAACAAGAC AACCCATCCT 
GCTAGGCCAC TCAAAAACCT TCCCATCGTA AACCAGTTTT CCACACTGTG CCTTGACCCT 
CTTGTTCAGC ACACTGCCCC TCTTTATGAA AGTGAAGAAC TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002664374.4
CDS	283..2727
Translation	MAQVEKKTSTMSVTVTSCSCETTSLSSTSSSSSSVLQQKHPHLVQPLCISPQLVQNNNQGSGNVGVSPTYVKCLHDVSTLKGQLVVLECRVRGTPPLQISWYREDKQIIDSADLRILRKKASSAFIPEELCTLVITEACPEDSGIFKCIASNQFGTVTCSAMLEVYTDLEEVLGDEQDPSSIQSDVLVKDDHCLVLQDTCAASPEWPKSSEEETATPMSWVLESGIKKVESLPDTDSEQSSVSKVAEDTNLSPMIKLDSPPKGVKNSVPHPFLLTSKLNKQVESKGSATNVADLPTFSPSLYPPSAFNYERPRHFIQSLPSFHTHDTETSKPNDLCVPATTPAPLVTPPLHAPTQTLMCSSMTLIPKARSTPNSENLSPAAFLSSVLPSPPSCQSKCLSIPQSPRHSSRSPSPTQKVQEPPLSPSNQLRNLPARIPPDTLKTTARSLQSSPVPHITYTSNMLPKPILKKNVSRPVSRATDEDIQGSKDALMQDLEQKLRNKVPQQRNNQQKISYEERMARRLLGADNAASVFHLENSFSSQPAQPGSPEDQYPGGIWSRKKRSGGDSTEASTIQEKCFAPRFIQVPQDVTVEEGRFCRIDFKVVGLPTPDISWYLDGKPIRPDDYHKMLVCEKSVHSFIIEIVTIHHAGLYECIAKNRAGQSQFSLHLDVIAQEQLCPPTFVVKMKNSHAMEGDAVRLECKVNASPSPQLYWKKDKEMLRIDHTRMSLSQDSSGKQCLVINPVMKSDAGWYTVSAINEAGMSTCNARLDVATRLNKTTHPARPLKNLPIVNQFSTLCLDPLVQHTAPLYESEEL

Target ORF information:

RefSeq Version	XM_002664328.6
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myotilin (myot), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002664328.6

ORF Insert Sequence:

ATGGCACAGG TTGAGAAAAA AACATCAACT ATGTCCGTAA CTGTAACATC ATGCTCCTGT 
GAAACAACCT CCTTGTCTTC AACATCATCT TCCTCTTCTT CAGTGCTTCA GCAAAAACAC 
CCCCATTTAG TACAGCCTCT CTGCATCAGT CCACAATTGG TTCAGAACAA TAACCAGGGT 
TCAGGAAATG TGGGAGTTTC TCCAACATAT GTCAAGTGTC TCCATGATGT CAGCACATTG 
AAGGGTCAGT TAGTGGTTCT GGAGTGCAGG GTCAGAGGAA CTCCTCCACT GCAGATCTCC 
TGGTATAGAG AAGACAAGCA AATCATTGAC TCTGCAGACT TACGCATTCT TAGAAAAAAG 
GCAAGTTCAG CTTTTATTCC GGAGGAATTG TGCACCCTAG TGATCACAGA GGCATGTCCA 
GAAGATTCTG GAATATTTAA ATGCATTGCA TCAAATCAGT TTGGAACAGT AACATGCAGT 
GCCATGCTGG AAGTATATAC AGACCTAGAA GAGGTGCTGG GAGATGAGCA AGACCCCTCC 
TCTATACAAT CAGATGTACT AGTAAAAGAT GACCATTGCT TAGTACTACA AGATACATGT 
GCCGCTTCTC CAGAATGGCC TAAAAGTTCA GAGGAAGAAA CTGCAACCCC TATGTCATGG 
GTACTGGAGT CTGGCATTAA AAAGGTTGAG TCTCTCCCAG ACACTGACAG CGAACAGAGT 
TCTGTCAGTA AGGTGGCTGA AGATACAAAC CTCTCCCCAA TGATCAAACT TGATTCTCCA 
CCAAAGGGAG TTAAAAACAG TGTTCCACAT CCATTCCTTC TCACCAGCAA GCTGAATAAA 
CAAGTAGAAT CCAAGGGCAG TGCCACGAAT GTGGCTGACC TGCCCACCTT TTCACCATCA 
TTGTACCCTC CAAGTGCTTT TAATTATGAG CGCCCTCGCC ATTTTATCCA GTCCCTGCCC 
AGTTTCCACA CACATGACAC TGAGACCAGC AAGCCAAATG ATCTTTGTGT TCCAGCTACA 
ACTCCTGCAC CCTTAGTGAC CCCTCCTCTT CATGCACCCA CTCAGACATT AATGTGTTCC 
AGCATGACTT TGATTCCTAA AGCAAGGAGT ACTCCAAACA GTGAGAATCT ATCACCAGCA 
GCTTTCCTAT CATCAGTCCT GCCTTCACCT CCAAGCTGCC AGTCAAAATG CCTTTCCATA 
CCTCAATCAC CCAGGCACTC CTCTAGATCA CCCAGCCCAA CACAAAAGGT CCAAGAACCA 
CCCCTATCTC CTTCAAATCA GTTACGGAAT CTCCCTGCCC GGATACCTCC AGATACATTG 
AAAACTACAG CTCGATCTCT GCAGTCTTCA CCTGTGCCCC ATATTACATA TACATCCAAC 
ATGTTGCCAA AACCAATCTT GAAGAAGAAT GTTTCACGAC CAGTGTCTCG TGCCACAGAT 
GAAGACATTC AGGGCTCTAA AGATGCTCTT ATGCAAGACC TTGAACAGAA GCTACGAAAC 
AAAGTTCCCC AACAAAGAAA CAATCAGCAG AAGATTTCTT ATGAGGAACG GATGGCCAGA 
AGATTGCTGG GTGCTGATAA TGCAGCTTCA GTTTTTCATC TCGAAAATTC CTTCAGTTCA 
CAGCCTGCAC AGCCAGGGTC TCCAGAAGAT CAGTACCCTG GAGGAATATG GTCAAGAAAA 
AAACGATCAG GGGGGGACAG TACAGAAGCC TCTACCATCC AAGAGAAATG TTTTGCTCCT 
CGTTTTATAC AAGTGCCTCA AGATGTTACT GTTGAAGAGG GACGATTCTG CAGGATTGAT 
TTTAAGGTTG TAGGTTTGCC AACCCCAGAT ATATCTTGGT ATCTGGATGG GAAACCCATT 
CGACCTGATG ACTATCATAA GATGCTGGTG TGTGAGAAAA GTGTCCACTC CTTCATTATT 
GAGATTGTAA CCATTCATCA TGCGGGTTTA TATGAATGTA TTGCAAAGAA CCGTGCTGGA 
CAGAGCCAAT TCTCCCTACA TTTGGATGTG ATTGCTCAGG AACAATTATG TCCTCCTACA 
TTTGTAGTGA AGATGAAGAA CTCACATGCA ATGGAGGGTG ATGCTGTTAG GTTAGAATGT 
AAAGTGAATG CATCTCCAAG CCCTCAGCTA TACTGGAAGA AAGACAAAGA AATGCTGCGT 
ATTGACCATA CGAGAATGAG TCTTTCTCAG GACAGTTCAG GAAAGCAGTG CCTGGTCATT 
AACCCAGTGA TGAAGTCTGA TGCTGGCTGG TATACTGTTT CCGCTATTAA TGAAGCAGGA 
ATGTCCACGT GCAATGCCAG GCTTGACGTA GCCACGCGAT TGAACAAGAC AACCCATCCT 
GCTAGGCCAC TCAAAAACCT TCCCATCGTA AACCAGTTTT CCACACTGTG CCTTGACCCT 
CTTGTTCAGC ACACTGCCCC TCTTTATGAA AGTGAAGAAC TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myot ( XM_009307788.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009306063.1 Danio rerio myotilin (myot), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa49255
Clone ID Related Accession (Same CDS sequence)	XM_009307788.2 , XM_009307788.3
Accession Version	XM_009307788.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	myotilin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307788.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCAAAC TTGATTCTCC ACCAAAGGGA GTTAAAAACA GTGTTCCACA TCCATTCCTT 
CTCACCAGCA AGCTGAATAA ACAAGTAGAA TCCAAGGGCA GTGCCACGAA TGTGGCTGAC 
CTGCCCACCT TTTCACCATC ATTGTACCCT CCAAGTGCTT TTAATTATGA GCGCCCTCGC 
CATTTTATCC AGTCCCTGCC CAGTTTCCAC ACACATGACA CTGAGACCAG CAAGCCAAAT 
GATCTTTGTG TTCCAGCTAC AACTCCTGCA CCCTTAGTGA CCCCTCCTCT TCATGCACCC 
ACTCAGACAT TAATGTGTTC CAGCATGACT TTGATTCCTA AAGCAAGGAG TACTCCAAAC 
AGTGAGAATC TATCACCAGC AGCTTTCCTA TCATCAGTCC TGCCTTCACC TCCAAGCTGC 
CAGTCAAAAT GCCTTTCCAT ACCTCAATCA CCCAGGCACT CCTCTAGATC ACCCAGCCCA 
ACACAAAAGG TCCAAGAACC ACCCCTATCT CCTTCAAATC AGTTACGGAA TCTCCCTGCC 
CGGATACCTC CAGATACATT GAAAACTACA GCTCGATCTC TGCAGTCTTC ACCTGTGCCC 
CATATTACAT ATACATCCAA CATGTTGCCA AAACCAATCT TGAAGAAGAA TGTTTCACGA 
CCAGTGTCTC GTGCCACAGA TGAAGACATT CAGGGCTCTA AAGATGCTCT TATGCAAGAC 
CTTGAACAGA AGCTACGAAA CAAAGTTCCC CAACAAAGAA ACAATCAGCA GAAGATTTCT 
TATGAGGAAC GGATGGCCAG AAGATTGCTG GGTGCTGATA ATGCAGCTTC AGTTTTTCAT 
CTCGAAAATT CCTTCAGTTC ACAGCCTGCA CAGCCAGGGT CTCCAGAAGA TCAGTACCCT 
GGAGGAATAT GGTCAAGAAA AAAACGATCA GGGGGGGACA GTACAGAAGC CTCTACCATC 
CAAGAGAAAT GTTTTGCTCC TCGTTTTATA CAAGTGCCTC AAGATGTTAC TGTTGAAGAG 
GGACGATTCT GCAGGATTGA TTTTAAGGTT GTAGGTTTGC CAACCCCAGA TATATCTTGG 
TATCTGGATG GGAAACCCAT TCGACCTGAT GACTATCATA AGATGCTGGT GTGTGAGAAA 
AGTGTCCACT CCTTCATTAT TGAGATTGTA ACCATTCATC ATGCGGGTTT ATATGAATGT 
ATTGCAAAGA ACCGTGCTGG ACAGAGCCAA TTCTCCCTAC ATTTGGATGT GATTGCTCAG 
GAACAATTAT GTCCTCCTAC ATTTGTAGTG AAGATGAAGA ACTCACATGC AATGGAGGGT 
GATGCTGTTA GGTTAGAATG TAAAGTGAAT GCATCTCCAA GCCCTCAGCT ATACTGGAAG 
AAAGACAAAG AAATGCTGCG TATTGACCAT ACGAGAATGA GTCTTTCTCA GGACAGTTCA 
GGAAAGCAGT GCCTGGTCAT TAACCCAGTG ATGAAGTCTG ATGCTGGCTG GTATACTGTT 
TCCGCTATTA ATGAAGCAGG AATGTCCACG TGCAATGCCA GGCTTGACGT AGCCACGCGA 
TTGAACAAGA CAACCCATCC TGCTAGGCCA CTCAAAAACC TTCCCATCGT AAACCAGTTT 
TCCACACTGT GCCTTGACCC TCTTGTTCAG CACACTGCCC CTCTTTATGA AAGTGAAGAA 
CTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009306063.1
CDS	217..1902
Translation	MIKLDSPPKGVKNSVPHPFLLTSKLNKQVESKGSATNVADLPTFSPSLYPPSAFNYERPRHFIQSLPSFHTHDTETSKPNDLCVPATTPAPLVTPPLHAPTQTLMCSSMTLIPKARSTPNSENLSPAAFLSSVLPSPPSCQSKCLSIPQSPRHSSRSPSPTQKVQEPPLSPSNQLRNLPARIPPDTLKTTARSLQSSPVPHITYTSNMLPKPILKKNVSRPVSRATDEDIQGSKDALMQDLEQKLRNKVPQQRNNQQKISYEERMARRLLGADNAASVFHLENSFSSQPAQPGSPEDQYPGGIWSRKKRSGGDSTEASTIQEKCFAPRFIQVPQDVTVEEGRFCRIDFKVVGLPTPDISWYLDGKPIRPDDYHKMLVCEKSVHSFIIEIVTIHHAGLYECIAKNRAGQSQFSLHLDVIAQEQLCPPTFVVKMKNSHAMEGDAVRLECKVNASPSPQLYWKKDKEMLRIDHTRMSLSQDSSGKQCLVINPVMKSDAGWYTVSAINEAGMSTCNARLDVATRLNKTTHPARPLKNLPIVNQFSTLCLDPLVQHTAPLYESEEL

Target ORF information:

RefSeq Version	XM_009307788.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio myotilin (myot), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307788.2

ORF Insert Sequence:

ATGATCAAAC TTGATTCTCC ACCAAAGGGA GTTAAAAACA GTGTTCCACA TCCATTCCTT 
CTCACCAGCA AGCTGAATAA ACAAGTAGAA TCCAAGGGCA GTGCCACGAA TGTGGCTGAC 
CTGCCCACCT TTTCACCATC ATTGTACCCT CCAAGTGCTT TTAATTATGA GCGCCCTCGC 
CATTTTATCC AGTCCCTGCC CAGTTTCCAC ACACATGACA CTGAGACCAG CAAGCCAAAT 
GATCTTTGTG TTCCAGCTAC AACTCCTGCA CCCTTAGTGA CCCCTCCTCT TCATGCACCC 
ACTCAGACAT TAATGTGTTC CAGCATGACT TTGATTCCTA AAGCAAGGAG TACTCCAAAC 
AGTGAGAATC TATCACCAGC AGCTTTCCTA TCATCAGTCC TGCCTTCACC TCCAAGCTGC 
CAGTCAAAAT GCCTTTCCAT ACCTCAATCA CCCAGGCACT CCTCTAGATC ACCCAGCCCA 
ACACAAAAGG TCCAAGAACC ACCCCTATCT CCTTCAAATC AGTTACGGAA TCTCCCTGCC 
CGGATACCTC CAGATACATT GAAAACTACA GCTCGATCTC TGCAGTCTTC ACCTGTGCCC 
CATATTACAT ATACATCCAA CATGTTGCCA AAACCAATCT TGAAGAAGAA TGTTTCACGA 
CCAGTGTCTC GTGCCACAGA TGAAGACATT CAGGGCTCTA AAGATGCTCT TATGCAAGAC 
CTTGAACAGA AGCTACGAAA CAAAGTTCCC CAACAAAGAA ACAATCAGCA GAAGATTTCT 
TATGAGGAAC GGATGGCCAG AAGATTGCTG GGTGCTGATA ATGCAGCTTC AGTTTTTCAT 
CTCGAAAATT CCTTCAGTTC ACAGCCTGCA CAGCCAGGGT CTCCAGAAGA TCAGTACCCT 
GGAGGAATAT GGTCAAGAAA AAAACGATCA GGGGGGGACA GTACAGAAGC CTCTACCATC 
CAAGAGAAAT GTTTTGCTCC TCGTTTTATA CAAGTGCCTC AAGATGTTAC TGTTGAAGAG 
GGACGATTCT GCAGGATTGA TTTTAAGGTT GTAGGTTTGC CAACCCCAGA TATATCTTGG 
TATCTGGATG GGAAACCCAT TCGACCTGAT GACTATCATA AGATGCTGGT GTGTGAGAAA 
AGTGTCCACT CCTTCATTAT TGAGATTGTA ACCATTCATC ATGCGGGTTT ATATGAATGT 
ATTGCAAAGA ACCGTGCTGG ACAGAGCCAA TTCTCCCTAC ATTTGGATGT GATTGCTCAG 
GAACAATTAT GTCCTCCTAC ATTTGTAGTG AAGATGAAGA ACTCACATGC AATGGAGGGT 
GATGCTGTTA GGTTAGAATG TAAAGTGAAT GCATCTCCAA GCCCTCAGCT ATACTGGAAG 
AAAGACAAAG AAATGCTGCG TATTGACCAT ACGAGAATGA GTCTTTCTCA GGACAGTTCA 
GGAAAGCAGT GCCTGGTCAT TAACCCAGTG ATGAAGTCTG ATGCTGGCTG GTATACTGTT 
TCCGCTATTA ATGAAGCAGG AATGTCCACG TGCAATGCCA GGCTTGACGT AGCCACGCGA 
TTGAACAAGA CAACCCATCC TGCTAGGCCA CTCAAAAACC TTCCCATCGT AAACCAGTTT 
TCCACACTGT GCCTTGACCC TCTTGTTCAG CACACTGCCC CTCTTTATGA AAGTGAAGAA 
CTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myot ( XM_009307788.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009306063.1 Danio rerio myotilin (myot), transcript variant X3, mRNA.

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CloneID	ODa49255
Clone ID Related Accession (Same CDS sequence)	XM_009307788.2 , XM_009307788.3
Accession Version	XM_009307788.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	myotilin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009307788.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCAAAC TTGATTCTCC ACCAAAGGGA GTTAAAAACA GTGTTCCACA TCCATTCCTT 
CTCACCAGCA AGCTGAATAA ACAAGTAGAA TCCAAGGGCA GTGCCACGAA TGTGGCTGAC 
CTGCCCACCT TTTCACCATC ATTGTACCCT CCAAGTGCTT TTAATTATGA GCGCCCTCGC 
CATTTTATCC AGTCCCTGCC CAGTTTCCAC ACACATGACA CTGAGACCAG CAAGCCAAAT 
GATCTTTGTG TTCCAGCTAC AACTCCTGCA CCCTTAGTGA CCCCTCCTCT TCATGCACCC 
ACTCAGACAT TAATGTGTTC CAGCATGACT TTGATTCCTA AAGCAAGGAG TACTCCAAAC 
AGTGAGAATC TATCACCAGC AGCTTTCCTA TCATCAGTCC TGCCTTCACC TCCAAGCTGC 
CAGTCAAAAT GCCTTTCCAT ACCTCAATCA CCCAGGCACT CCTCTAGATC ACCCAGCCCA 
ACACAAAAGG TCCAAGAACC ACCCCTATCT CCTTCAAATC AGTTACGGAA TCTCCCTGCC 
CGGATACCTC CAGATACATT GAAAACTACA GCTCGATCTC TGCAGTCTTC ACCTGTGCCC 
CATATTACAT ATACATCCAA CATGTTGCCA AAACCAATCT TGAAGAAGAA TGTTTCACGA 
CCAGTGTCTC GTGCCACAGA TGAAGACATT CAGGGCTCTA AAGATGCTCT TATGCAAGAC 
CTTGAACAGA AGCTACGAAA CAAAGTTCCC CAACAAAGAA ACAATCAGCA GAAGATTTCT 
TATGAGGAAC GGATGGCCAG AAGATTGCTG GGTGCTGATA ATGCAGCTTC AGTTTTTCAT 
CTCGAAAATT CCTTCAGTTC ACAGCCTGCA CAGCCAGGGT CTCCAGAAGA TCAGTACCCT 
GGAGGAATAT GGTCAAGAAA AAAACGATCA GGGGGGGACA GTACAGAAGC CTCTACCATC 
CAAGAGAAAT GTTTTGCTCC TCGTTTTATA CAAGTGCCTC AAGATGTTAC TGTTGAAGAG 
GGACGATTCT GCAGGATTGA TTTTAAGGTT GTAGGTTTGC CAACCCCAGA TATATCTTGG 
TATCTGGATG GGAAACCCAT TCGACCTGAT GACTATCATA AGATGCTGGT GTGTGAGAAA 
AGTGTCCACT CCTTCATTAT TGAGATTGTA ACCATTCATC ATGCGGGTTT ATATGAATGT 
ATTGCAAAGA ACCGTGCTGG ACAGAGCCAA TTCTCCCTAC ATTTGGATGT GATTGCTCAG 
GAACAATTAT GTCCTCCTAC ATTTGTAGTG AAGATGAAGA ACTCACATGC AATGGAGGGT 
GATGCTGTTA GGTTAGAATG TAAAGTGAAT GCATCTCCAA GCCCTCAGCT ATACTGGAAG 
AAAGACAAAG AAATGCTGCG TATTGACCAT ACGAGAATGA GTCTTTCTCA GGACAGTTCA 
GGAAAGCAGT GCCTGGTCAT TAACCCAGTG ATGAAGTCTG ATGCTGGCTG GTATACTGTT 
TCCGCTATTA ATGAAGCAGG AATGTCCACG TGCAATGCCA GGCTTGACGT AGCCACGCGA 
TTGAACAAGA CAACCCATCC TGCTAGGCCA CTCAAAAACC TTCCCATCGT AAACCAGTTT 
TCCACACTGT GCCTTGACCC TCTTGTTCAG CACACTGCCC CTCTTTATGA AAGTGAAGAA 
CTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009306063.1
CDS	232..1917
Translation	MIKLDSPPKGVKNSVPHPFLLTSKLNKQVESKGSATNVADLPTFSPSLYPPSAFNYERPRHFIQSLPSFHTHDTETSKPNDLCVPATTPAPLVTPPLHAPTQTLMCSSMTLIPKARSTPNSENLSPAAFLSSVLPSPPSCQSKCLSIPQSPRHSSRSPSPTQKVQEPPLSPSNQLRNLPARIPPDTLKTTARSLQSSPVPHITYTSNMLPKPILKKNVSRPVSRATDEDIQGSKDALMQDLEQKLRNKVPQQRNNQQKISYEERMARRLLGADNAASVFHLENSFSSQPAQPGSPEDQYPGGIWSRKKRSGGDSTEASTIQEKCFAPRFIQVPQDVTVEEGRFCRIDFKVVGLPTPDISWYLDGKPIRPDDYHKMLVCEKSVHSFIIEIVTIHHAGLYECIAKNRAGQSQFSLHLDVIAQEQLCPPTFVVKMKNSHAMEGDAVRLECKVNASPSPQLYWKKDKEMLRIDHTRMSLSQDSSGKQCLVINPVMKSDAGWYTVSAINEAGMSTCNARLDVATRLNKTTHPARPLKNLPIVNQFSTLCLDPLVQHTAPLYESEEL

Target ORF information:

RefSeq Version	XM_009307788.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myotilin (myot), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307788.3

ORF Insert Sequence:

ATGATCAAAC TTGATTCTCC ACCAAAGGGA GTTAAAAACA GTGTTCCACA TCCATTCCTT 
CTCACCAGCA AGCTGAATAA ACAAGTAGAA TCCAAGGGCA GTGCCACGAA TGTGGCTGAC 
CTGCCCACCT TTTCACCATC ATTGTACCCT CCAAGTGCTT TTAATTATGA GCGCCCTCGC 
CATTTTATCC AGTCCCTGCC CAGTTTCCAC ACACATGACA CTGAGACCAG CAAGCCAAAT 
GATCTTTGTG TTCCAGCTAC AACTCCTGCA CCCTTAGTGA CCCCTCCTCT TCATGCACCC 
ACTCAGACAT TAATGTGTTC CAGCATGACT TTGATTCCTA AAGCAAGGAG TACTCCAAAC 
AGTGAGAATC TATCACCAGC AGCTTTCCTA TCATCAGTCC TGCCTTCACC TCCAAGCTGC 
CAGTCAAAAT GCCTTTCCAT ACCTCAATCA CCCAGGCACT CCTCTAGATC ACCCAGCCCA 
ACACAAAAGG TCCAAGAACC ACCCCTATCT CCTTCAAATC AGTTACGGAA TCTCCCTGCC 
CGGATACCTC CAGATACATT GAAAACTACA GCTCGATCTC TGCAGTCTTC ACCTGTGCCC 
CATATTACAT ATACATCCAA CATGTTGCCA AAACCAATCT TGAAGAAGAA TGTTTCACGA 
CCAGTGTCTC GTGCCACAGA TGAAGACATT CAGGGCTCTA AAGATGCTCT TATGCAAGAC 
CTTGAACAGA AGCTACGAAA CAAAGTTCCC CAACAAAGAA ACAATCAGCA GAAGATTTCT 
TATGAGGAAC GGATGGCCAG AAGATTGCTG GGTGCTGATA ATGCAGCTTC AGTTTTTCAT 
CTCGAAAATT CCTTCAGTTC ACAGCCTGCA CAGCCAGGGT CTCCAGAAGA TCAGTACCCT 
GGAGGAATAT GGTCAAGAAA AAAACGATCA GGGGGGGACA GTACAGAAGC CTCTACCATC 
CAAGAGAAAT GTTTTGCTCC TCGTTTTATA CAAGTGCCTC AAGATGTTAC TGTTGAAGAG 
GGACGATTCT GCAGGATTGA TTTTAAGGTT GTAGGTTTGC CAACCCCAGA TATATCTTGG 
TATCTGGATG GGAAACCCAT TCGACCTGAT GACTATCATA AGATGCTGGT GTGTGAGAAA 
AGTGTCCACT CCTTCATTAT TGAGATTGTA ACCATTCATC ATGCGGGTTT ATATGAATGT 
ATTGCAAAGA ACCGTGCTGG ACAGAGCCAA TTCTCCCTAC ATTTGGATGT GATTGCTCAG 
GAACAATTAT GTCCTCCTAC ATTTGTAGTG AAGATGAAGA ACTCACATGC AATGGAGGGT 
GATGCTGTTA GGTTAGAATG TAAAGTGAAT GCATCTCCAA GCCCTCAGCT ATACTGGAAG 
AAAGACAAAG AAATGCTGCG TATTGACCAT ACGAGAATGA GTCTTTCTCA GGACAGTTCA 
GGAAAGCAGT GCCTGGTCAT TAACCCAGTG ATGAAGTCTG ATGCTGGCTG GTATACTGTT 
TCCGCTATTA ATGAAGCAGG AATGTCCACG TGCAATGCCA GGCTTGACGT AGCCACGCGA 
TTGAACAAGA CAACCCATCC TGCTAGGCCA CTCAAAAACC TTCCCATCGT AAACCAGTTT 
TCCACACTGT GCCTTGACCC TCTTGTTCAG CACACTGCCC CTCTTTATGA AAGTGAAGAA 
CTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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myot cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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