CCDC57 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	CCDC57
Entrez Gene ID	100318317
Full Name	coiled-coil domain containing 57
General protein information	Preferred Names coiled-coil domain containing 57 Names coiled-coil domain-containing protein 57 zmp:0000001288
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009306774.3	XP_009305049.1	coiled-coil domain-containing protein 57 isoform X1
NM_001201562.2	NP_001188491.2	coiled-coil domain-containing protein 57
XM_009306774.2	XP_009305049.1	coiled-coil domain-containing protein 57 isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	ccdc57	NP_001188491.1
Pan troglodytes (chimpanzee)	CCDC57	XP_003953284.1
Macaca mulatta (Rhesus monkey)	CCDC57	XP_001113581.1
Mus musculus (house mouse)	Ccdc57	NP_082021.1
Homo sapiens (human)	CCDC57	NP_932348.2
Xenopus tropicalis (tropical clawed frog)	ccdc57	XP_002937358.2
Canis lupus familiaris (dog)	CCDC57	XP_005624031.1
Bos taurus (cattle)	CCDC57	XP_005221152.1
Danio rerio (zebrafish)	LOC100538128	XP_003199676.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CCDC57 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CCDC57 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa18077	NM_001201562.2 Latest version!	Danio rerio coiled-coil domain containing 57 (ccdc57), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa18076	XM_009306774.3 Latest version!	Danio rerio coiled-coil domain containing 57 (ccdc57), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
ODa18076	XM_009306774.2	Danio rerio coiled-coil domain containing 57 (ccdc57), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CCDC57 ( NM_001201562.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001188491.2 Danio rerio coiled-coil domain containing 57 (ccdc57), mRNA.

Price & Availability↑

CloneID	ODa18077
Clone ID Related Accession (Same CDS sequence)	NM_001201562.2
Accession Version	NM_001201562.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2940bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1542902400000
Organism	Danio rerio(zebrafish)
Product	coiled-coil domain-containing protein 57
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR932358.4. On Nov 22, 2014 this sequence version replaced NM_001201562.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGGAAG CTGTGGACCT GGAGTCCCAG CTGGCCTGTA AAGAAAGAGA GTGGAAAGAA 
CTTCAGGGTT TACGGATTCG GCAACTGGAT ACTGCTCTAA ATGAAGCCAC ATCAGAGCTG 
TACGACCAGA GAGAGCGTTT CCTCCGCTTG CGTGATGACT TTAAATATAA TCTGCGTGTG 
TTGGAGGAAA GGGACAAAGA GCTGGAGCGT TATGATGCCA TGGCAGCTCG TGCTCAGATG 
GAGGAGAGCG CGAGGCGGGA AGAGGTCAGT GAGCTTAGGA TTGAGATAGC GAAGCTTCAG 
AATGCCCTAG AGGAGCAGCG GAGGGCGAAG GAGGACATCG AAGTGCAATT TCAGAAAAGA 
GGAGTTGAGC ATAGAGTCAA AGTAGAAAAA GTCCAGAGCA AAATGGAAAG TGAGATTCAG 
AAGCTAAGGG AGGAGAATGA ATCACTGAGA CGAGATCTCC AGCGACGAAT TAGAGAATCT 
GATGGAGAAC TGGCACTGCA GAAACAGGAA ATGATGGCAG ATTTTGACAG TGAAATGAGA 
AAACGAGAGC ATGAATTTCA CTTGAAGCTG GATGAAATGA ACAACCTGGT TCTCTCCTGT 
CAGCTCAAAG AAAAACTATT GAGCAAAGAG TTAGAGGTCC ATGCCAAAGC ACACAGCCAA 
GCAACTGAAG CCCTGCAGGC CTCTGAAGAA CTTTACCAGC AGGCTCAAAA AGAGATCCAG 
CATCGAGACT GGGACCTAAA AAACACCACC GCAATGAAAG ACTCCAGGAT AAAGGAGCTT 
GAGGAAACAC TGAAACAACT AGAGAACAAC TGTAAAAAAG AACAGGAAGC CTACGACAGG 
AAGCATGCAG AGCTGGAACG AAGGACTCGG GAGAGCAAAG ATGCATTGGA CCGCTTGAGA 
GAAGCTCATG CCAGGGATCT CCAAGAGGAG AGGGAGAAAT CCTCAGAATT ACAAGCCCAA 
CTGGACAGGA TGATCCTGGA GCTGGAGAGG AGAGAAAAAA CCCGTACAGA TGACCTGGAA 
CATAGAGACC AGCAGATACA AGAGCTCAGA GCACAGCTGG AAACAACTCG ATCTGGCTGG 
GATACTTACA TCATGCAGGT CTCCAAAGAA AAGGTTGCCA GGGATACAGA GCTGTTTTCC 
GCAGGGGAGA GAGAGGCCAA AGTGAGAGCA GAGCTGGAAA GGTGTAAAGA GGATATAGAA 
AGGTACAAGC AGCAGGTGTC AAGTGGTCTG CAGAGGGAAC AGGCACTGGA GCAGAAGAGA 
GTTCAGCTAG AGTTGGACTG GGAGAGACGC TGTGAGGACG TGCGTTCTGA GCACTACCTG 
AGGAGCGAGG AGATCATCCA AAACCTTACT CAGGCCCGAG ACCAGGTGAC TGCTGAGTTG 
AGAGAGAAGG AACGTGAGTT GCAGGAGACG ATGACAGTGC TTCAAAGTGT CACTGTAGAG 
CGAGATCAGG CTCTGTGTGG AAACAAACCA GCACTAACTG GATTTCAGAC ATCTGTAATA 
GATGGCAGCA GTTTTCCATC AGAGGAGATC AGGAGACTCC AGCAGCAGAA CAACACTCTA 
AAAGCAGTGG TGGCTGAAAT GAGGAAAGAG ATGGAGATCC TGAGCAAACA GAGGCTTGCA 
GCTCCAAGAC CGGCCACAAA TCTAGAGCCT AGCACTGCAG GGTCTACAGA CTACACTAAG 
GCTTTGGAGA AAGAGATTCT GGAGCTGAAG GCCAAATGTC GAAATCTTGA GGAGCGTTTA 
GAGGAGTCTT CCAAGACCGT CAACACTTCC ATCAATCCTA CCCCTGCGTT TCCTGTTTCT 
CCAGACAATG CTTACCTTCA GAACCACATT AGATCACTTA ATGAGACCAT AGGTGGCCTA 
CGAGTGGAGA AAGTTGCCAA TGCCGCTGCT CTAAAGAAAC AGGAAGTGAG ACTGGCTCAC 
TTGGAGTCAA CTGTGGAACA GCTTACCCAG CAGTGTCACT CAAAACATAT GGAGAACGAG 
AGCTTGTGTT TAGAGCTGGC CAATCACAAG AGGGCAGCAA CGGCTGAAGA AGCAAGGCTT 
AAACAGAGAG TGGCGGCTGC TGAGCTGGAG CTGAATGAAG TGAGGCGGGA GGCAGAAGAA 
CACCAGAAAG GCAGTTTGCT TCACAACCTG GAGACTGTTG CTCTTGGAAA CCAGGTGTCG 
GCACTGAAGG TTGACCTTGC AAGCAGAAGG GAACCCATTG TCCTTAATCA GAGTGAGATG 
GTAAAGCAGC TTCAGGAGGA GAACCTGTCC CTCAGAAAGC AACTGCTGCT TCTGCACAGC 
TCATCCAGAG GAAGCAGCAT AGGGGACGTT CCTCTGCTTC AGTCAAAGCT GAAACAGGCT 
GCACGTATGA TTTCTGGTCT CAGTCAGGAC AAACAGCAAC TCATTGAAAT GGGCAACAGG 
CTGCGGGCTC AGCTCATAGA AGCAGGCCTG GACGTTCCAC GACACTCAAA GATCATTCTT 
CAACCAAACA CGCCAGAGCC CAATCTGGCA GAAAAAGACC CATCTCAACC AGAGCACGGG 
ATTCAGTCAA AGAGCCGCTT ATCTACCCTG GAACAGCTGC AGTACCAGCT AACCACTCAG 
TCTGTGAGCA GTAAAGAAAA CACTCCGCCA CAACAGAATA AGCCTGAAGC TCTGGTACGA 
CACCACATTC TGTCATCTAT AAGCACCGAT GAGTCCCTTC AGAAAGTGTG GCAGATGTTG 
GATCAAGGTT TAAGTTCCTC TGTGTTTTCT CCTAGTGACA GTGAGGACAA AGGTAGAGTT 
TCTACAGCTG ATAGAGTACC TGATATTACT CAGCCTCCAG CTCCACTGGT CAGCGTGGAG 
GGCAAGACAG CATCACTCCG GGAGAAGAAG AAACAGAGTC GAACAGCATC TGTACCTGCT 
AAAAAGGCGA TTCCTCCTGG AAAACACGCT AAGATTCGAA ATTACAATAT CAAAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188491.2
CDS	363..3302
Translation	MLEAVDLESQLACKEREWKELQGLRIRQLDTALNEATSELYDQRERFLRLRDDFKYNLRVLEERDKELERYDAMAARAQMEESARREEVSELRIEIAKLQNALEEQRRAKEDIEVQFQKRGVEHRVKVEKVQSKMESEIQKLREENESLRRDLQRRIRESDGELALQKQEMMADFDSEMRKREHEFHLKLDEMNNLVLSCQLKEKLLSKELEVHAKAHSQATEALQASEELYQQAQKEIQHRDWDLKNTTAMKDSRIKELEETLKQLENNCKKEQEAYDRKHAELERRTRESKDALDRLREAHARDLQEEREKSSELQAQLDRMILELERREKTRTDDLEHRDQQIQELRAQLETTRSGWDTYIMQVSKEKVARDTELFSAGEREAKVRAELERCKEDIERYKQQVSSGLQREQALEQKRVQLELDWERRCEDVRSEHYLRSEEIIQNLTQARDQVTAELREKERELQETMTVLQSVTVERDQALCGNKPALTGFQTSVIDGSSFPSEEIRRLQQQNNTLKAVVAEMRKEMEILSKQRLAAPRPATNLEPSTAGSTDYTKALEKEILELKAKCRNLEERLEESSKTVNTSINPTPAFPVSPDNAYLQNHIRSLNETIGGLRVEKVANAAALKKQEVRLAHLESTVEQLTQQCHSKHMENESLCLELANHKRAATAEEARLKQRVAAAELELNEVRREAEEHQKGSLLHNLETVALGNQVSALKVDLASRREPIVLNQSEMVKQLQEENLSLRKQLLLLHSSSRGSSIGDVPLLQSKLKQAARMISGLSQDKQQLIEMGNRLRAQLIEAGLDVPRHSKIILQPNTPEPNLAEKDPSQPEHGIQSKSRLSTLEQLQYQLTTQSVSSKENTPPQQNKPEALVRHHILSSISTDESLQKVWQMLDQGLSSSVFSPSDSEDKGRVSTADRVPDITQPPAPLVSVEGKTASLREKKKQSRTASVPAKKAIPPGKHAKIRNYNIKD

Target ORF information:

RefSeq Version	NM_001201562.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio coiled-coil domain containing 57 (ccdc57), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201562.2

ORF Insert Sequence:

ATGCTGGAAG CTGTGGACCT GGAGTCCCAG CTGGCCTGTA AAGAAAGAGA GTGGAAAGAA 
CTTCAGGGTT TACGGATTCG GCAACTGGAT ACTGCTCTAA ATGAAGCCAC ATCAGAGCTG 
TACGACCAGA GAGAGCGTTT CCTCCGCTTG CGTGATGACT TTAAATATAA TCTGCGTGTG 
TTGGAGGAAA GGGACAAAGA GCTGGAGCGT TATGATGCCA TGGCAGCTCG TGCTCAGATG 
GAGGAGAGCG CGAGGCGGGA AGAGGTCAGT GAGCTTAGGA TTGAGATAGC GAAGCTTCAG 
AATGCCCTAG AGGAGCAGCG GAGGGCGAAG GAGGACATCG AAGTGCAATT TCAGAAAAGA 
GGAGTTGAGC ATAGAGTCAA AGTAGAAAAA GTCCAGAGCA AAATGGAAAG TGAGATTCAG 
AAGCTAAGGG AGGAGAATGA ATCACTGAGA CGAGATCTCC AGCGACGAAT TAGAGAATCT 
GATGGAGAAC TGGCACTGCA GAAACAGGAA ATGATGGCAG ATTTTGACAG TGAAATGAGA 
AAACGAGAGC ATGAATTTCA CTTGAAGCTG GATGAAATGA ACAACCTGGT TCTCTCCTGT 
CAGCTCAAAG AAAAACTATT GAGCAAAGAG TTAGAGGTCC ATGCCAAAGC ACACAGCCAA 
GCAACTGAAG CCCTGCAGGC CTCTGAAGAA CTTTACCAGC AGGCTCAAAA AGAGATCCAG 
CATCGAGACT GGGACCTAAA AAACACCACC GCAATGAAAG ACTCCAGGAT AAAGGAGCTT 
GAGGAAACAC TGAAACAACT AGAGAACAAC TGTAAAAAAG AACAGGAAGC CTACGACAGG 
AAGCATGCAG AGCTGGAACG AAGGACTCGG GAGAGCAAAG ATGCATTGGA CCGCTTGAGA 
GAAGCTCATG CCAGGGATCT CCAAGAGGAG AGGGAGAAAT CCTCAGAATT ACAAGCCCAA 
CTGGACAGGA TGATCCTGGA GCTGGAGAGG AGAGAAAAAA CCCGTACAGA TGACCTGGAA 
CATAGAGACC AGCAGATACA AGAGCTCAGA GCACAGCTGG AAACAACTCG ATCTGGCTGG 
GATACTTACA TCATGCAGGT CTCCAAAGAA AAGGTTGCCA GGGATACAGA GCTGTTTTCC 
GCAGGGGAGA GAGAGGCCAA AGTGAGAGCA GAGCTGGAAA GGTGTAAAGA GGATATAGAA 
AGGTACAAGC AGCAGGTGTC AAGTGGTCTG CAGAGGGAAC AGGCACTGGA GCAGAAGAGA 
GTTCAGCTAG AGTTGGACTG GGAGAGACGC TGTGAGGACG TGCGTTCTGA GCACTACCTG 
AGGAGCGAGG AGATCATCCA AAACCTTACT CAGGCCCGAG ACCAGGTGAC TGCTGAGTTG 
AGAGAGAAGG AACGTGAGTT GCAGGAGACG ATGACAGTGC TTCAAAGTGT CACTGTAGAG 
CGAGATCAGG CTCTGTGTGG AAACAAACCA GCACTAACTG GATTTCAGAC ATCTGTAATA 
GATGGCAGCA GTTTTCCATC AGAGGAGATC AGGAGACTCC AGCAGCAGAA CAACACTCTA 
AAAGCAGTGG TGGCTGAAAT GAGGAAAGAG ATGGAGATCC TGAGCAAACA GAGGCTTGCA 
GCTCCAAGAC CGGCCACAAA TCTAGAGCCT AGCACTGCAG GGTCTACAGA CTACACTAAG 
GCTTTGGAGA AAGAGATTCT GGAGCTGAAG GCCAAATGTC GAAATCTTGA GGAGCGTTTA 
GAGGAGTCTT CCAAGACCGT CAACACTTCC ATCAATCCTA CCCCTGCGTT TCCTGTTTCT 
CCAGACAATG CTTACCTTCA GAACCACATT AGATCACTTA ATGAGACCAT AGGTGGCCTA 
CGAGTGGAGA AAGTTGCCAA TGCCGCTGCT CTAAAGAAAC AGGAAGTGAG ACTGGCTCAC 
TTGGAGTCAA CTGTGGAACA GCTTACCCAG CAGTGTCACT CAAAACATAT GGAGAACGAG 
AGCTTGTGTT TAGAGCTGGC CAATCACAAG AGGGCAGCAA CGGCTGAAGA AGCAAGGCTT 
AAACAGAGAG TGGCGGCTGC TGAGCTGGAG CTGAATGAAG TGAGGCGGGA GGCAGAAGAA 
CACCAGAAAG GCAGTTTGCT TCACAACCTG GAGACTGTTG CTCTTGGAAA CCAGGTGTCG 
GCACTGAAGG TTGACCTTGC AAGCAGAAGG GAACCCATTG TCCTTAATCA GAGTGAGATG 
GTAAAGCAGC TTCAGGAGGA GAACCTGTCC CTCAGAAAGC AACTGCTGCT TCTGCACAGC 
TCATCCAGAG GAAGCAGCAT AGGGGACGTT CCTCTGCTTC AGTCAAAGCT GAAACAGGCT 
GCACGTATGA TTTCTGGTCT CAGTCAGGAC AAACAGCAAC TCATTGAAAT GGGCAACAGG 
CTGCGGGCTC AGCTCATAGA AGCAGGCCTG GACGTTCCAC GACACTCAAA GATCATTCTT 
CAACCAAACA CGCCAGAGCC CAATCTGGCA GAAAAAGACC CATCTCAACC AGAGCACGGG 
ATTCAGTCAA AGAGCCGCTT ATCTACCCTG GAACAGCTGC AGTACCAGCT AACCACTCAG 
TCTGTGAGCA GTAAAGAAAA CACTCCGCCA CAACAGAATA AGCCTGAAGC TCTGGTACGA 
CACCACATTC TGTCATCTAT AAGCACCGAT GAGTCCCTTC AGAAAGTGTG GCAGATGTTG 
GATCAAGGTT TAAGTTCCTC TGTGTTTTCT CCTAGTGACA GTGAGGACAA AGGTAGAGTT 
TCTACAGCTG ATAGAGTACC TGATATTACT CAGCCTCCAG CTCCACTGGT CAGCGTGGAG 
GGCAAGACAG CATCACTCCG GGAGAAGAAG AAACAGAGTC GAACAGCATC TGTACCTGCT 
AAAAAGGCGA TTCCTCCTGG AAAACACGCT AAGATTCGAA ATTACAATAT CAAAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CCDC57 ( XM_009306774.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009305049.1 Danio rerio coiled-coil domain containing 57 (ccdc57), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa18076
Clone ID Related Accession (Same CDS sequence)	XM_009306774.3 , XM_009306774.2
Accession Version	XM_009306774.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3069bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	coiled-coil domain-containing protein 57 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306774.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGAAG CTGTGGACCT GGAGTCCCAG CTGGCCTGTA AAGAAAGAGA GTGGAAAGAA 
CTTCAGGGTT TACGGATTCG GCAACTGGAT ACTGCTCTAA ATGAAGCCAC ATCAGAGCTG 
TACGACCAGA GAGAGCGTTT CCTCCGCTTG CGTGATGACT TTAAATATAA TCTGCGTGTG 
TTGGAGGAAA GGGACAAAGA GCTGGAGCGT TATGATGCCA TGGCAGCTCG TGCTCAGATG 
GAGGAGAGCG CGAGGCGGGA AGAGGTCAGT GAGCTTAGGA TTGAGATAGC GAAGCTTCAG 
AATGCCCTAG AGGAGCAGCG GAGGGCGAAG GAGGACATCG AAGTGCAATT TCAGAAAAGA 
GGAGTTGAGC ATAGAGTCAA AGTAGAAAAA GTCCAGAGCA AAATGGAAAG TGAGATTCAG 
AAGCTAAGGG AGGAGAATGA ATCACTGAGA CGAGATCTCC AGCGACGAAT TAGAGAATCT 
GATGGAGAAC TGGCACTGCA GAAACAGGAA ATGATGGCAG ATTTTGACAG TGAAATGAGA 
AAACGAGAGC ATGAATTTCA CTTGAAGCTG GATGAAATGA ACAACCTGGT TCTCTCCTGT 
CAGCTCAAAG AAAAACTATT GAGCAAAGAG TTAGAGGTCC ATGCCAAAGC ACACAGCCAA 
GCAACTGAAG CCCTGCAGGC CTCTGAAGAA CTTTACCAGC AGGCTCAAAA AGAGATCCAG 
CATCGAGACT GGGACCTAAA AAACACCACC GCAATGAAAG ACTCCAGGAT AAAGGAGCTT 
GAGGAAACAC TGAAACAACT AGAGAACAAC TGTAAAAAAG AACAGGAAGC CTACGACAGG 
AAGCATGCAG AGCTGGAACG AAGGACTCGG GAGAGCAAAG ATGCATTGGA CCGCTTGAGA 
GAAGCTCATG CCAGGGATCT CCAAGAGGAG AGGGAGAAAT CCTCAGAATT ACAAGCCCAA 
CTGGACAGGA TGATCCTGGA GCTGGAGAGG AGAGAAAAAA CCCGTACAGA TGACCTGGAA 
CATAGAGACC AGCAGATACA AGAGCTCAGA GCACAGCTGG AAACAACTCG ATCTGGCTGG 
GATACTTACA TCATGCAGGT CTCCAAAGAA AAGGTTGCCA GGGATACAGA GCTGTTTTCC 
GCAGGGGAGA GAGAGGCCAA AGTGAGAGCA GAGCTGGAAA GGTGTAAAGA GGATATAGAA 
AGGTACAAGC AGCAGGTGTC AAGTGGTCTG CAGAGGGAAC AGGCACTGGA GCAGAAGAGA 
GTTCAGCTAG AGTTGGACTG GGAGAGACGC TGTGAGGACG TGCGTTCTGA GCACTACCTG 
AGGAGCGAGG AGATCATCCA AAACCTTACT CAGGCCCGAG ACCAGGTGAC TGCTGAGTTG 
AGAGAGAAGG AACGTGAGTT GCAGGAGACG ATGACAGTGC TTCAAAGTGT CACTGTAGAG 
CGAGATCAGG CTCTGTGTGG AAACAAACCA GCACTAACTG GATTTCAGAC ATCTGTAATA 
GATGGCAGCA GTTTTCCATC AGAGGAGATC AGGAGACTCC AGCAGCAGAA CAACACTCTA 
AAAGCAGTGG TGGCTGAAAT GAGGAAAGAG ATGGAGATCC TGAGCAAACA GAGGCTTGCA 
GCTCCAAGAC CGGCCACAAA TCTAGAGCCT AGCACTGCAG GGTCTACAGA CTACACTAAG 
GCTTTGGAGA AAGAGATTCT GGAGCTGAAG GCCAAATGTC GAAATCTTGA GGAGCGTTTA 
GAGGAGTCTT CCAAGACCGT CAACACTTCC ATCAATCCTA CCCCTGCGTT TCCTGTTTCT 
CCAGACAATG CTTACCTTCA GAACCACATT AGATCACTTA ATGAGACCAT AGGTGGCCTA 
CGAGTGGAGA AAGTTGCCAA TGCCGCTGCT CTAAAGAAAC AGGAAGTGAG ACTGGCTCAC 
TTGGAGTCAA CTGTGGAACA GCTTACCCAG CAGTGTCACT CAAAACATAT GGAGAACGAG 
AGCTTGTGTT TAGAGCTGGC CAATCACAAG AGGGCAGCAA CGGCTGAAGA AGCAAGGCTT 
AAACAGAGAG TGGCGGCTGC TGAGCTGGAG CTGAATGAAG TGAGGCGGGA GGCAGAAGAA 
CACCAGAAAG GCAGTTTGCT TCACAACCTG GAGACTGTTG CTCTTGGAAA CCAGGTGTCG 
GCACTGAAGG TTGACCTTGC AAGCAGAAGG GAACCCATTG TCCTTAATCA GAGTGAGATG 
GTAAAGCAGC TTCAGGAGGA GAACCTGTCC CTCAGAAAGC AACTGCTGCT TCTGCACAGC 
TCATCCAGAG GAAGCAGCAT AGGGGACGTT CCTCTGCTTC AGTCAAAGCT GAAACAGGCT 
GCACGTATGA TTTCTGGTCT CAGTCAGGAC AAACAGCAAC TCATTGAAAT GGGCAACAGG 
CTGCGGGCTC AGCTCATAGA AGCAGGCCTG GACGTTCCAC GACACTCAAA GATCATTCTT 
CAACCAAACA CGCCAGAGCC CAATCTGGCA GAAAAAGACC CATCTCAACC AGAGCACGGG 
ATTCAGTCAA AGAGCCGCTT ATCTACCCTG GAACAGCTGC AGTACCAGCT AACCACTCAG 
GAGCTACAGT ATGCACAGAG AGATCAAAAT AAGAAAACGG CAATTATTGT CCGTCCTCAG 
TTGTCTGAGA GTGAGAGTTC AGACAAACAC AGAACCACCA ACCCATGGGA GCCATCAGTC 
AGAGTCCAGT CTGTGAGCAG TAAAGAAAAC ACTCCGCCAC AACAGAATAA GCCTGAAGCT 
CTGGTACGAC ACCACATTCT GTCATCTATA AGCACCGATG AGTCCCTTCA GAAAGTGTGG 
CAGATGTTGG ATCAAGGTTT AAGTTCCTCT GTGTTTTCTC CTAGTGACAG TGAGGACAAA 
GGTAGAGTTT CTACAGCTGA TAGAGTACCT GATATTACTC AGCCTCCAGC TCCACTGGTC 
AGCGTGGAGG GCAAGACAGC ATCACTCCGG GAGAAGAAGA AACAGAGTCG AACAGCATCT 
GTACCTGCTA AAAAGGCGAT TCCTCCTGGA AAACACGCTA AGATTCGAAA TTACAATATC 
AAAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305049.1
CDS	362..3430
Translation	MLEAVDLESQLACKEREWKELQGLRIRQLDTALNEATSELYDQRERFLRLRDDFKYNLRVLEERDKELERYDAMAARAQMEESARREEVSELRIEIAKLQNALEEQRRAKEDIEVQFQKRGVEHRVKVEKVQSKMESEIQKLREENESLRRDLQRRIRESDGELALQKQEMMADFDSEMRKREHEFHLKLDEMNNLVLSCQLKEKLLSKELEVHAKAHSQATEALQASEELYQQAQKEIQHRDWDLKNTTAMKDSRIKELEETLKQLENNCKKEQEAYDRKHAELERRTRESKDALDRLREAHARDLQEEREKSSELQAQLDRMILELERREKTRTDDLEHRDQQIQELRAQLETTRSGWDTYIMQVSKEKVARDTELFSAGEREAKVRAELERCKEDIERYKQQVSSGLQREQALEQKRVQLELDWERRCEDVRSEHYLRSEEIIQNLTQARDQVTAELREKERELQETMTVLQSVTVERDQALCGNKPALTGFQTSVIDGSSFPSEEIRRLQQQNNTLKAVVAEMRKEMEILSKQRLAAPRPATNLEPSTAGSTDYTKALEKEILELKAKCRNLEERLEESSKTVNTSINPTPAFPVSPDNAYLQNHIRSLNETIGGLRVEKVANAAALKKQEVRLAHLESTVEQLTQQCHSKHMENESLCLELANHKRAATAEEARLKQRVAAAELELNEVRREAEEHQKGSLLHNLETVALGNQVSALKVDLASRREPIVLNQSEMVKQLQEENLSLRKQLLLLHSSSRGSSIGDVPLLQSKLKQAARMISGLSQDKQQLIEMGNRLRAQLIEAGLDVPRHSKIILQPNTPEPNLAEKDPSQPEHGIQSKSRLSTLEQLQYQLTTQELQYAQRDQNKKTAIIVRPQLSESESSDKHRTTNPWEPSVRVQSVSSKENTPPQQNKPEALVRHHILSSISTDESLQKVWQMLDQGLSSSVFSPSDSEDKGRVSTADRVPDITQPPAPLVSVEGKTASLREKKKQSRTASVPAKKAIPPGKHAKIRNYNIKD

Target ORF information:

RefSeq Version	XM_009306774.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio coiled-coil domain containing 57 (ccdc57), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306774.3

ORF Insert Sequence:

ATGCTGGAAG CTGTGGACCT GGAGTCCCAG CTGGCCTGTA AAGAAAGAGA GTGGAAAGAA 
CTTCAGGGTT TACGGATTCG GCAACTGGAT ACTGCTCTAA ATGAAGCCAC ATCAGAGCTG 
TACGACCAGA GAGAGCGTTT CCTCCGCTTG CGTGATGACT TTAAATATAA TCTGCGTGTG 
TTGGAGGAAA GGGACAAAGA GCTGGAGCGT TATGATGCCA TGGCAGCTCG TGCTCAGATG 
GAGGAGAGCG CGAGGCGGGA AGAGGTCAGT GAGCTTAGGA TTGAGATAGC GAAGCTTCAG 
AATGCCCTAG AGGAGCAGCG GAGGGCGAAG GAGGACATCG AAGTGCAATT TCAGAAAAGA 
GGAGTTGAGC ATAGAGTCAA AGTAGAAAAA GTCCAGAGCA AAATGGAAAG TGAGATTCAG 
AAGCTAAGGG AGGAGAATGA ATCACTGAGA CGAGATCTCC AGCGACGAAT TAGAGAATCT 
GATGGAGAAC TGGCACTGCA GAAACAGGAA ATGATGGCAG ATTTTGACAG TGAAATGAGA 
AAACGAGAGC ATGAATTTCA CTTGAAGCTG GATGAAATGA ACAACCTGGT TCTCTCCTGT 
CAGCTCAAAG AAAAACTATT GAGCAAAGAG TTAGAGGTCC ATGCCAAAGC ACACAGCCAA 
GCAACTGAAG CCCTGCAGGC CTCTGAAGAA CTTTACCAGC AGGCTCAAAA AGAGATCCAG 
CATCGAGACT GGGACCTAAA AAACACCACC GCAATGAAAG ACTCCAGGAT AAAGGAGCTT 
GAGGAAACAC TGAAACAACT AGAGAACAAC TGTAAAAAAG AACAGGAAGC CTACGACAGG 
AAGCATGCAG AGCTGGAACG AAGGACTCGG GAGAGCAAAG ATGCATTGGA CCGCTTGAGA 
GAAGCTCATG CCAGGGATCT CCAAGAGGAG AGGGAGAAAT CCTCAGAATT ACAAGCCCAA 
CTGGACAGGA TGATCCTGGA GCTGGAGAGG AGAGAAAAAA CCCGTACAGA TGACCTGGAA 
CATAGAGACC AGCAGATACA AGAGCTCAGA GCACAGCTGG AAACAACTCG ATCTGGCTGG 
GATACTTACA TCATGCAGGT CTCCAAAGAA AAGGTTGCCA GGGATACAGA GCTGTTTTCC 
GCAGGGGAGA GAGAGGCCAA AGTGAGAGCA GAGCTGGAAA GGTGTAAAGA GGATATAGAA 
AGGTACAAGC AGCAGGTGTC AAGTGGTCTG CAGAGGGAAC AGGCACTGGA GCAGAAGAGA 
GTTCAGCTAG AGTTGGACTG GGAGAGACGC TGTGAGGACG TGCGTTCTGA GCACTACCTG 
AGGAGCGAGG AGATCATCCA AAACCTTACT CAGGCCCGAG ACCAGGTGAC TGCTGAGTTG 
AGAGAGAAGG AACGTGAGTT GCAGGAGACG ATGACAGTGC TTCAAAGTGT CACTGTAGAG 
CGAGATCAGG CTCTGTGTGG AAACAAACCA GCACTAACTG GATTTCAGAC ATCTGTAATA 
GATGGCAGCA GTTTTCCATC AGAGGAGATC AGGAGACTCC AGCAGCAGAA CAACACTCTA 
AAAGCAGTGG TGGCTGAAAT GAGGAAAGAG ATGGAGATCC TGAGCAAACA GAGGCTTGCA 
GCTCCAAGAC CGGCCACAAA TCTAGAGCCT AGCACTGCAG GGTCTACAGA CTACACTAAG 
GCTTTGGAGA AAGAGATTCT GGAGCTGAAG GCCAAATGTC GAAATCTTGA GGAGCGTTTA 
GAGGAGTCTT CCAAGACCGT CAACACTTCC ATCAATCCTA CCCCTGCGTT TCCTGTTTCT 
CCAGACAATG CTTACCTTCA GAACCACATT AGATCACTTA ATGAGACCAT AGGTGGCCTA 
CGAGTGGAGA AAGTTGCCAA TGCCGCTGCT CTAAAGAAAC AGGAAGTGAG ACTGGCTCAC 
TTGGAGTCAA CTGTGGAACA GCTTACCCAG CAGTGTCACT CAAAACATAT GGAGAACGAG 
AGCTTGTGTT TAGAGCTGGC CAATCACAAG AGGGCAGCAA CGGCTGAAGA AGCAAGGCTT 
AAACAGAGAG TGGCGGCTGC TGAGCTGGAG CTGAATGAAG TGAGGCGGGA GGCAGAAGAA 
CACCAGAAAG GCAGTTTGCT TCACAACCTG GAGACTGTTG CTCTTGGAAA CCAGGTGTCG 
GCACTGAAGG TTGACCTTGC AAGCAGAAGG GAACCCATTG TCCTTAATCA GAGTGAGATG 
GTAAAGCAGC TTCAGGAGGA GAACCTGTCC CTCAGAAAGC AACTGCTGCT TCTGCACAGC 
TCATCCAGAG GAAGCAGCAT AGGGGACGTT CCTCTGCTTC AGTCAAAGCT GAAACAGGCT 
GCACGTATGA TTTCTGGTCT CAGTCAGGAC AAACAGCAAC TCATTGAAAT GGGCAACAGG 
CTGCGGGCTC AGCTCATAGA AGCAGGCCTG GACGTTCCAC GACACTCAAA GATCATTCTT 
CAACCAAACA CGCCAGAGCC CAATCTGGCA GAAAAAGACC CATCTCAACC AGAGCACGGG 
ATTCAGTCAA AGAGCCGCTT ATCTACCCTG GAACAGCTGC AGTACCAGCT AACCACTCAG 
GAGCTACAGT ATGCACAGAG AGATCAAAAT AAGAAAACGG CAATTATTGT CCGTCCTCAG 
TTGTCTGAGA GTGAGAGTTC AGACAAACAC AGAACCACCA ACCCATGGGA GCCATCAGTC 
AGAGTCCAGT CTGTGAGCAG TAAAGAAAAC ACTCCGCCAC AACAGAATAA GCCTGAAGCT 
CTGGTACGAC ACCACATTCT GTCATCTATA AGCACCGATG AGTCCCTTCA GAAAGTGTGG 
CAGATGTTGG ATCAAGGTTT AAGTTCCTCT GTGTTTTCTC CTAGTGACAG TGAGGACAAA 
GGTAGAGTTT CTACAGCTGA TAGAGTACCT GATATTACTC AGCCTCCAGC TCCACTGGTC 
AGCGTGGAGG GCAAGACAGC ATCACTCCGG GAGAAGAAGA AACAGAGTCG AACAGCATCT 
GTACCTGCTA AAAAGGCGAT TCCTCCTGGA AAACACGCTA AGATTCGAAA TTACAATATC 
AAAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CCDC57 ( XM_009306774.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009305049.1 Danio rerio coiled-coil domain containing 57 (ccdc57), transcript variant X1, mRNA.

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CloneID	ODa18076
Clone ID Related Accession (Same CDS sequence)	XM_009306774.3 , XM_009306774.2
Accession Version	XM_009306774.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3069bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	coiled-coil domain-containing protein 57 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306774.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGAAG CTGTGGACCT GGAGTCCCAG CTGGCCTGTA AAGAAAGAGA GTGGAAAGAA 
CTTCAGGGTT TACGGATTCG GCAACTGGAT ACTGCTCTAA ATGAAGCCAC ATCAGAGCTG 
TACGACCAGA GAGAGCGTTT CCTCCGCTTG CGTGATGACT TTAAATATAA TCTGCGTGTG 
TTGGAGGAAA GGGACAAAGA GCTGGAGCGT TATGATGCCA TGGCAGCTCG TGCTCAGATG 
GAGGAGAGCG CGAGGCGGGA AGAGGTCAGT GAGCTTAGGA TTGAGATAGC GAAGCTTCAG 
AATGCCCTAG AGGAGCAGCG GAGGGCGAAG GAGGACATCG AAGTGCAATT TCAGAAAAGA 
GGAGTTGAGC ATAGAGTCAA AGTAGAAAAA GTCCAGAGCA AAATGGAAAG TGAGATTCAG 
AAGCTAAGGG AGGAGAATGA ATCACTGAGA CGAGATCTCC AGCGACGAAT TAGAGAATCT 
GATGGAGAAC TGGCACTGCA GAAACAGGAA ATGATGGCAG ATTTTGACAG TGAAATGAGA 
AAACGAGAGC ATGAATTTCA CTTGAAGCTG GATGAAATGA ACAACCTGGT TCTCTCCTGT 
CAGCTCAAAG AAAAACTATT GAGCAAAGAG TTAGAGGTCC ATGCCAAAGC ACACAGCCAA 
GCAACTGAAG CCCTGCAGGC CTCTGAAGAA CTTTACCAGC AGGCTCAAAA AGAGATCCAG 
CATCGAGACT GGGACCTAAA AAACACCACC GCAATGAAAG ACTCCAGGAT AAAGGAGCTT 
GAGGAAACAC TGAAACAACT AGAGAACAAC TGTAAAAAAG AACAGGAAGC CTACGACAGG 
AAGCATGCAG AGCTGGAACG AAGGACTCGG GAGAGCAAAG ATGCATTGGA CCGCTTGAGA 
GAAGCTCATG CCAGGGATCT CCAAGAGGAG AGGGAGAAAT CCTCAGAATT ACAAGCCCAA 
CTGGACAGGA TGATCCTGGA GCTGGAGAGG AGAGAAAAAA CCCGTACAGA TGACCTGGAA 
CATAGAGACC AGCAGATACA AGAGCTCAGA GCACAGCTGG AAACAACTCG ATCTGGCTGG 
GATACTTACA TCATGCAGGT CTCCAAAGAA AAGGTTGCCA GGGATACAGA GCTGTTTTCC 
GCAGGGGAGA GAGAGGCCAA AGTGAGAGCA GAGCTGGAAA GGTGTAAAGA GGATATAGAA 
AGGTACAAGC AGCAGGTGTC AAGTGGTCTG CAGAGGGAAC AGGCACTGGA GCAGAAGAGA 
GTTCAGCTAG AGTTGGACTG GGAGAGACGC TGTGAGGACG TGCGTTCTGA GCACTACCTG 
AGGAGCGAGG AGATCATCCA AAACCTTACT CAGGCCCGAG ACCAGGTGAC TGCTGAGTTG 
AGAGAGAAGG AACGTGAGTT GCAGGAGACG ATGACAGTGC TTCAAAGTGT CACTGTAGAG 
CGAGATCAGG CTCTGTGTGG AAACAAACCA GCACTAACTG GATTTCAGAC ATCTGTAATA 
GATGGCAGCA GTTTTCCATC AGAGGAGATC AGGAGACTCC AGCAGCAGAA CAACACTCTA 
AAAGCAGTGG TGGCTGAAAT GAGGAAAGAG ATGGAGATCC TGAGCAAACA GAGGCTTGCA 
GCTCCAAGAC CGGCCACAAA TCTAGAGCCT AGCACTGCAG GGTCTACAGA CTACACTAAG 
GCTTTGGAGA AAGAGATTCT GGAGCTGAAG GCCAAATGTC GAAATCTTGA GGAGCGTTTA 
GAGGAGTCTT CCAAGACCGT CAACACTTCC ATCAATCCTA CCCCTGCGTT TCCTGTTTCT 
CCAGACAATG CTTACCTTCA GAACCACATT AGATCACTTA ATGAGACCAT AGGTGGCCTA 
CGAGTGGAGA AAGTTGCCAA TGCCGCTGCT CTAAAGAAAC AGGAAGTGAG ACTGGCTCAC 
TTGGAGTCAA CTGTGGAACA GCTTACCCAG CAGTGTCACT CAAAACATAT GGAGAACGAG 
AGCTTGTGTT TAGAGCTGGC CAATCACAAG AGGGCAGCAA CGGCTGAAGA AGCAAGGCTT 
AAACAGAGAG TGGCGGCTGC TGAGCTGGAG CTGAATGAAG TGAGGCGGGA GGCAGAAGAA 
CACCAGAAAG GCAGTTTGCT TCACAACCTG GAGACTGTTG CTCTTGGAAA CCAGGTGTCG 
GCACTGAAGG TTGACCTTGC AAGCAGAAGG GAACCCATTG TCCTTAATCA GAGTGAGATG 
GTAAAGCAGC TTCAGGAGGA GAACCTGTCC CTCAGAAAGC AACTGCTGCT TCTGCACAGC 
TCATCCAGAG GAAGCAGCAT AGGGGACGTT CCTCTGCTTC AGTCAAAGCT GAAACAGGCT 
GCACGTATGA TTTCTGGTCT CAGTCAGGAC AAACAGCAAC TCATTGAAAT GGGCAACAGG 
CTGCGGGCTC AGCTCATAGA AGCAGGCCTG GACGTTCCAC GACACTCAAA GATCATTCTT 
CAACCAAACA CGCCAGAGCC CAATCTGGCA GAAAAAGACC CATCTCAACC AGAGCACGGG 
ATTCAGTCAA AGAGCCGCTT ATCTACCCTG GAACAGCTGC AGTACCAGCT AACCACTCAG 
GAGCTACAGT ATGCACAGAG AGATCAAAAT AAGAAAACGG CAATTATTGT CCGTCCTCAG 
TTGTCTGAGA GTGAGAGTTC AGACAAACAC AGAACCACCA ACCCATGGGA GCCATCAGTC 
AGAGTCCAGT CTGTGAGCAG TAAAGAAAAC ACTCCGCCAC AACAGAATAA GCCTGAAGCT 
CTGGTACGAC ACCACATTCT GTCATCTATA AGCACCGATG AGTCCCTTCA GAAAGTGTGG 
CAGATGTTGG ATCAAGGTTT AAGTTCCTCT GTGTTTTCTC CTAGTGACAG TGAGGACAAA 
GGTAGAGTTT CTACAGCTGA TAGAGTACCT GATATTACTC AGCCTCCAGC TCCACTGGTC 
AGCGTGGAGG GCAAGACAGC ATCACTCCGG GAGAAGAAGA AACAGAGTCG AACAGCATCT 
GTACCTGCTA AAAAGGCGAT TCCTCCTGGA AAACACGCTA AGATTCGAAA TTACAATATC 
AAAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305049.1
CDS	359..3427
Translation	MLEAVDLESQLACKEREWKELQGLRIRQLDTALNEATSELYDQRERFLRLRDDFKYNLRVLEERDKELERYDAMAARAQMEESARREEVSELRIEIAKLQNALEEQRRAKEDIEVQFQKRGVEHRVKVEKVQSKMESEIQKLREENESLRRDLQRRIRESDGELALQKQEMMADFDSEMRKREHEFHLKLDEMNNLVLSCQLKEKLLSKELEVHAKAHSQATEALQASEELYQQAQKEIQHRDWDLKNTTAMKDSRIKELEETLKQLENNCKKEQEAYDRKHAELERRTRESKDALDRLREAHARDLQEEREKSSELQAQLDRMILELERREKTRTDDLEHRDQQIQELRAQLETTRSGWDTYIMQVSKEKVARDTELFSAGEREAKVRAELERCKEDIERYKQQVSSGLQREQALEQKRVQLELDWERRCEDVRSEHYLRSEEIIQNLTQARDQVTAELREKERELQETMTVLQSVTVERDQALCGNKPALTGFQTSVIDGSSFPSEEIRRLQQQNNTLKAVVAEMRKEMEILSKQRLAAPRPATNLEPSTAGSTDYTKALEKEILELKAKCRNLEERLEESSKTVNTSINPTPAFPVSPDNAYLQNHIRSLNETIGGLRVEKVANAAALKKQEVRLAHLESTVEQLTQQCHSKHMENESLCLELANHKRAATAEEARLKQRVAAAELELNEVRREAEEHQKGSLLHNLETVALGNQVSALKVDLASRREPIVLNQSEMVKQLQEENLSLRKQLLLLHSSSRGSSIGDVPLLQSKLKQAARMISGLSQDKQQLIEMGNRLRAQLIEAGLDVPRHSKIILQPNTPEPNLAEKDPSQPEHGIQSKSRLSTLEQLQYQLTTQELQYAQRDQNKKTAIIVRPQLSESESSDKHRTTNPWEPSVRVQSVSSKENTPPQQNKPEALVRHHILSSISTDESLQKVWQMLDQGLSSSVFSPSDSEDKGRVSTADRVPDITQPPAPLVSVEGKTASLREKKKQSRTASVPAKKAIPPGKHAKIRNYNIKD

Target ORF information:

RefSeq Version	XM_009306774.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio coiled-coil domain containing 57 (ccdc57), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306774.2

ORF Insert Sequence:

ATGCTGGAAG CTGTGGACCT GGAGTCCCAG CTGGCCTGTA AAGAAAGAGA GTGGAAAGAA 
CTTCAGGGTT TACGGATTCG GCAACTGGAT ACTGCTCTAA ATGAAGCCAC ATCAGAGCTG 
TACGACCAGA GAGAGCGTTT CCTCCGCTTG CGTGATGACT TTAAATATAA TCTGCGTGTG 
TTGGAGGAAA GGGACAAAGA GCTGGAGCGT TATGATGCCA TGGCAGCTCG TGCTCAGATG 
GAGGAGAGCG CGAGGCGGGA AGAGGTCAGT GAGCTTAGGA TTGAGATAGC GAAGCTTCAG 
AATGCCCTAG AGGAGCAGCG GAGGGCGAAG GAGGACATCG AAGTGCAATT TCAGAAAAGA 
GGAGTTGAGC ATAGAGTCAA AGTAGAAAAA GTCCAGAGCA AAATGGAAAG TGAGATTCAG 
AAGCTAAGGG AGGAGAATGA ATCACTGAGA CGAGATCTCC AGCGACGAAT TAGAGAATCT 
GATGGAGAAC TGGCACTGCA GAAACAGGAA ATGATGGCAG ATTTTGACAG TGAAATGAGA 
AAACGAGAGC ATGAATTTCA CTTGAAGCTG GATGAAATGA ACAACCTGGT TCTCTCCTGT 
CAGCTCAAAG AAAAACTATT GAGCAAAGAG TTAGAGGTCC ATGCCAAAGC ACACAGCCAA 
GCAACTGAAG CCCTGCAGGC CTCTGAAGAA CTTTACCAGC AGGCTCAAAA AGAGATCCAG 
CATCGAGACT GGGACCTAAA AAACACCACC GCAATGAAAG ACTCCAGGAT AAAGGAGCTT 
GAGGAAACAC TGAAACAACT AGAGAACAAC TGTAAAAAAG AACAGGAAGC CTACGACAGG 
AAGCATGCAG AGCTGGAACG AAGGACTCGG GAGAGCAAAG ATGCATTGGA CCGCTTGAGA 
GAAGCTCATG CCAGGGATCT CCAAGAGGAG AGGGAGAAAT CCTCAGAATT ACAAGCCCAA 
CTGGACAGGA TGATCCTGGA GCTGGAGAGG AGAGAAAAAA CCCGTACAGA TGACCTGGAA 
CATAGAGACC AGCAGATACA AGAGCTCAGA GCACAGCTGG AAACAACTCG ATCTGGCTGG 
GATACTTACA TCATGCAGGT CTCCAAAGAA AAGGTTGCCA GGGATACAGA GCTGTTTTCC 
GCAGGGGAGA GAGAGGCCAA AGTGAGAGCA GAGCTGGAAA GGTGTAAAGA GGATATAGAA 
AGGTACAAGC AGCAGGTGTC AAGTGGTCTG CAGAGGGAAC AGGCACTGGA GCAGAAGAGA 
GTTCAGCTAG AGTTGGACTG GGAGAGACGC TGTGAGGACG TGCGTTCTGA GCACTACCTG 
AGGAGCGAGG AGATCATCCA AAACCTTACT CAGGCCCGAG ACCAGGTGAC TGCTGAGTTG 
AGAGAGAAGG AACGTGAGTT GCAGGAGACG ATGACAGTGC TTCAAAGTGT CACTGTAGAG 
CGAGATCAGG CTCTGTGTGG AAACAAACCA GCACTAACTG GATTTCAGAC ATCTGTAATA 
GATGGCAGCA GTTTTCCATC AGAGGAGATC AGGAGACTCC AGCAGCAGAA CAACACTCTA 
AAAGCAGTGG TGGCTGAAAT GAGGAAAGAG ATGGAGATCC TGAGCAAACA GAGGCTTGCA 
GCTCCAAGAC CGGCCACAAA TCTAGAGCCT AGCACTGCAG GGTCTACAGA CTACACTAAG 
GCTTTGGAGA AAGAGATTCT GGAGCTGAAG GCCAAATGTC GAAATCTTGA GGAGCGTTTA 
GAGGAGTCTT CCAAGACCGT CAACACTTCC ATCAATCCTA CCCCTGCGTT TCCTGTTTCT 
CCAGACAATG CTTACCTTCA GAACCACATT AGATCACTTA ATGAGACCAT AGGTGGCCTA 
CGAGTGGAGA AAGTTGCCAA TGCCGCTGCT CTAAAGAAAC AGGAAGTGAG ACTGGCTCAC 
TTGGAGTCAA CTGTGGAACA GCTTACCCAG CAGTGTCACT CAAAACATAT GGAGAACGAG 
AGCTTGTGTT TAGAGCTGGC CAATCACAAG AGGGCAGCAA CGGCTGAAGA AGCAAGGCTT 
AAACAGAGAG TGGCGGCTGC TGAGCTGGAG CTGAATGAAG TGAGGCGGGA GGCAGAAGAA 
CACCAGAAAG GCAGTTTGCT TCACAACCTG GAGACTGTTG CTCTTGGAAA CCAGGTGTCG 
GCACTGAAGG TTGACCTTGC AAGCAGAAGG GAACCCATTG TCCTTAATCA GAGTGAGATG 
GTAAAGCAGC TTCAGGAGGA GAACCTGTCC CTCAGAAAGC AACTGCTGCT TCTGCACAGC 
TCATCCAGAG GAAGCAGCAT AGGGGACGTT CCTCTGCTTC AGTCAAAGCT GAAACAGGCT 
GCACGTATGA TTTCTGGTCT CAGTCAGGAC AAACAGCAAC TCATTGAAAT GGGCAACAGG 
CTGCGGGCTC AGCTCATAGA AGCAGGCCTG GACGTTCCAC GACACTCAAA GATCATTCTT 
CAACCAAACA CGCCAGAGCC CAATCTGGCA GAAAAAGACC CATCTCAACC AGAGCACGGG 
ATTCAGTCAA AGAGCCGCTT ATCTACCCTG GAACAGCTGC AGTACCAGCT AACCACTCAG 
GAGCTACAGT ATGCACAGAG AGATCAAAAT AAGAAAACGG CAATTATTGT CCGTCCTCAG 
TTGTCTGAGA GTGAGAGTTC AGACAAACAC AGAACCACCA ACCCATGGGA GCCATCAGTC 
AGAGTCCAGT CTGTGAGCAG TAAAGAAAAC ACTCCGCCAC AACAGAATAA GCCTGAAGCT 
CTGGTACGAC ACCACATTCT GTCATCTATA AGCACCGATG AGTCCCTTCA GAAAGTGTGG 
CAGATGTTGG ATCAAGGTTT AAGTTCCTCT GTGTTTTCTC CTAGTGACAG TGAGGACAAA 
GGTAGAGTTT CTACAGCTGA TAGAGTACCT GATATTACTC AGCCTCCAGC TCCACTGGTC 
AGCGTGGAGG GCAAGACAGC ATCACTCCGG GAGAAGAAGA AACAGAGTCG AACAGCATCT 
GTACCTGCTA AAAAGGCGAT TCCTCCTGGA AAACACGCTA AGATTCGAAA TTACAATATC 
AAAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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CCDC57 cDNA ORF clone, Danio rerio(Zebrafish)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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