COL12A1A cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL12A1A
Entrez Gene ID	100149543
Full Name	collagen, type XII, alpha 1a
Synonyms	col12a1,wu:fa99f12
General protein information	Preferred Names collagen, type XII, alpha 1a Names collagen alpha-1(XII) chain collagen, type XII, alpha 1 fa99f12
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009293297.3	XP_009291572.1	collagen alpha-1(XII) chain isoform X3
XM_009293297.2	XP_009291572.1	collagen alpha-1(XII) chain isoform X3
XM_002665259.6	XP_002665305.3	collagen alpha-1(XII) chain isoform X2
XM_009293298.3	XP_009291573.1	collagen alpha-1(XII) chain isoform X4
XM_002665259.5	XP_002665305.3	collagen alpha-1(XII) chain isoform X2
XM_009293296.2	XP_009291571.1	collagen alpha-1(XII) chain isoform X1
XM_009293296.3	XP_009291571.1	collagen alpha-1(XII) chain isoform X1
XM_009293298.2	XP_009291573.1	collagen alpha-1(XII) chain isoform X4

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Col12a1	XP_006243503.1
Danio rerio (zebrafish)	col12a1a	XP_002665305.3
Canis lupus familiaris (dog)	COL12A1	XP_005627591.1
Gallus gallus (chicken)	COL12A1	NP_990352.1
Homo sapiens (human)	COL12A1	NP_004361.3
Pan troglodytes (chimpanzee)	COL12A1	XP_001142912.1
Xenopus tropicalis (tropical clawed frog)	col12a1	XP_002936246.2
Macaca mulatta (Rhesus monkey)	COL12A1	XP_001109727.2
Bos taurus (cattle)	COL12A1	NP_001193426.1
Mus musculus (house mouse)	Col12a1	NP_031756.2

Gene Ontology

The following COL12A1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL12A1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa48774	XM_009293298.3 Latest version!	Danio rerio collagen, type XII, alpha 1a (col12a1a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa22912	XM_002665259.6 Latest version!	Danio rerio collagen, type XII, alpha 1a (col12a1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa48772	XM_009293296.3 Latest version!	Danio rerio collagen, type XII, alpha 1a (col12a1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa48773	XM_009293297.3 Latest version!	Danio rerio collagen, type XII, alpha 1a (col12a1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
ODa48773	XM_009293297.2	Danio rerio collagen, type XII, alpha 1a (col12a1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa22912	XM_002665259.5	Danio rerio collagen, type XII, alpha 1a (col12a1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa48772	XM_009293296.2	Danio rerio collagen, type XII, alpha 1a (col12a1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa48774	XM_009293298.2	Danio rerio collagen, type XII, alpha 1a (col12a1a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL12A1A ( XM_009293298.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009291573.1 Danio rerio collagen, type XII, alpha 1a (col12a1a), transcript variant X4, mRNA.

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CloneID	ODa48774
Clone ID Related Accession (Same CDS sequence)	XM_009293298.3 , XM_009293298.2
Accession Version	XM_009293298.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	collagen alpha-1(XII) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293298.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATCA GGTTGTCTTT GGCCGCTGCT GCGCTCTTGA CTCTAGTACT CAGTTCAGTA 
GAGGCTCAAG ATGCCCAGTG TAAAACCACG GCCCAAGCGG ATATCGTCCT GCTTGTTGAT 
GGATCTTGGA GCATTGGTCG CCTGAACTTC AAGACCATAC GCAATTTCAT TGCTCGCATG 
GTGGGAGTCT TCGACATCGG CCCCGATCGT GTCCAGATTG GTCTGGCACA GTACAGCGGA 
GACCCGAAGA CTGAGTGGCA TCTCAATGCT CACAGAACCA GGACTCAGCT GCTGGATGCG 
GTGGCCAATC TACCCTACAA AGGAGGAAAC ACTCTCACCG GCCTTGCTTT GAATTACATC 
CTTCAGAATA ACTTCAAGCC TAACGTTGGC ATGCGTCCAA ACTCCAGAAA GATCGGCGTC 
CTGGTCACTG ATGGCAAGTC CCAAGACGAC ATTGTGGCGA ACTCACAGAA CCTGCGGGAC 
CAAGGGATTG AACTGTACGC CATTGGTGTA AAGAACGCAG ATGAGAATGA GTTGAGAACC 
ATTGCTTCCG ATCCCGATGA CATTCACATG TACAACGTGG CTGATTTCTC CTTCCTGCTG 
GACATTGTGG ATGACCTCAC AAACAACCTG TGCAACAGCG TCAAAGGTCC AGGAGGAGGA 
CCAGAGCCAC CCACTGATCT GACCACCTCA GAGGTCACAC ATTACTCTTT CCGTGCCACT 
TGGGTGGCTC CTGACGGGCC TGTGGAGAAG TACAGAGTGG AGTATGTGAG CACCGCTGGA 
GGAGAACCAC AGCAACAGAT GTTTGTGGAC GGGACGGTGA CCACTGTGGT TTTGGAAGGT 
CTCACTCCGC TGACCGAGTA CATAGTAAAG GTTTACTCAG TGGTGGGAGA GGACAGCAGC 
GACCCACTCA GAGGCATAGA GACCACATTG CCTCTCAGTG CTGTGAGGAG CATGAACATC 
TACAAAGAGC GCTCCACCAC CATGAATGTG AGATGGGACG CAGCCACAGG GGCCACAGGC 
TACATGCTTC TCTACAAGGC CCTTAACGCC ACTGAACCCT CTGTGGAGCA GGAGATGCGA 
GTGAAAGGAA TGGTCACTGA CGTCCAGCTG AAGAAACTTC TCCCCAACAC GGCCTACAGT 
GTTTCGCTCT TCGCCCTGCA CGGCGATTAC GCCAGTGAAC CTCTGATTGA TCAAGGGGTG 
ACCCTGCCTC TTCCTCCATC CGGTGAGTTG AGGATTACCG ATGTGACCCA CAGCACCATG 
CAGCTGGACT GGGACGCTGC GCCCGGCGCC GTCCGAAAGT ACATCATCAC TTACAAGCCA 
GAGGAGGGAG AGGCGAAGGA GCTTGAAGTT GACGGAGATG TAACATCTAG AGGCCTGGAT 
AATCTCATCT CCCAGACTGA ATATGATGTG GCTGTGACTC CGGTTTATGA TTCGGGCACT 
GGAAACCCCA TGCTTAACCA GGCCATTACA GATGTGGTTC CTGCCCCGCA GAACTTGAGG 
TTCTCGATGG TGACCCAGAC CAGTTTTAGG GTTCACTGGG AGCACGGCGC TCCTGATGTC 
TCCCTCTACC GTATTGGCTG GAAAAAGAAA GGAACGACCG AGTTTCAAAA TGATATCCTG 
AGTAGTGAAG ACACTACATA TGATCTGGTG GACCTGGAGC CAGACACACC TTATGATGTT 
TCGGTCACTG CCATTTACCC TGATGAGTCT GAGAGCGAGG ATCTGCTGGG CACTGAACGC 
ACTTTGGCCA AGCTTGCTAC ACCACCTCCC AGTGGTCCTC CTCAAAACCT CCAGGTGTTC 
GACGAGACCA CCACCTCCCT CACGACTAAG TGGGACCACG CGCCTGGACG AGTTCAGAAC 
TACAAAATCA CCTACGTACC GCTTACTGGA GGACGATCAC AGTCGACGCA GGTCGGAGGA 
AAGAAAAACA CAGTGATCCT GCAGAAACTG ACTCCAGACA CTCCTTACTC CATCACTGTT 
GCTGCGGTCT ACGCCAGCGG AGAGGCCAGA GACATTTCTG GCCAAGGAAA GACATTGCCG 
CTGGGCAGTG TCAGGAATCT TCAGGTCACT GACCCCACAG TTAGCACTCT GAATGTGCGC 
TGGGAGCCGG CGGAGGGAAG CGTTCGCCAG TACAGGATCT ACTACCAGCC CGTCGCAGGG 
GGCGCAGAGG ACATGGTCCA GGTTCCTGGA AGCACCACCA GCACCGTTCT GAAGAACCTG 
CAATCTGACA CTCAGTACAG AGTGACGGTG GTGCCAGTCT ACGCTCCTGG AGAGGGCAAG 
CGCATGTCGG AGACCGGAAA AACACTGGTT CGGACCCCTG CCAAGAACAT CCAGGTTTAC 
AACCCCACCA CAAACACCCT GAACGTGCGC TGGGAGCCGG CCTCAGGACA GGTGCAGCAG 
TACCGCGTGG CTTACGCCCC TCTCTCCGGG ATCCGACCCT CTCAGTCGGT GCTGGTTCCA 
GGAAACACCC ACACGGCTCT GCTGCAGCAG CTGGAATCAG ACACTGACTA CTCTGTGAGT 
GTGGTGGCTC TGTATGCTGA TGGAGAGGGC TCGTCTGTGT CTGATAATGG CAAAACACTG 
CCCCGCAGTG GCCCCAGGAA CCTGCGGGTT TATGATCCCA CCACCAGCAC GCTGTCGGTG 
GGCTGGGAAC ATGCAGAAGG GCCTGTGATT CAGTACCGCA TTGGCTACGC ACCCACCACT 
GGAGATCCCA TTGAAGAATT TACAACTGTC CCAGGCAACC GTAACTCGGT CATCCTGCAA 
AACCTGCAGC CAGACACGCC GTACAACATC AACGTAGAAG CCATTTATCA AGATGGCCCT 
GGAGGACGAC TCAGTGGAGA TGGAAAAACA TTGGATCTCC TTGGGCCTCG TAACCTGCGT 
GTTTCAGACG AGTGGTACAC ACGTTTCCGA GTGTCCTGGG ATCCTGCAGC CGCACGGGTG 
AATGGATATA AACTGGTCTA TACACCAGTG GGCACAACCG ACACCATGGA AGCCTTCGTT 
GGAGACGTGA CTTCACACCA CCTGAACAAC CTCCAGCCTG GCACCACATA CGACCTGAAG 
GTTTACGCTC AGTATGATGC GGGCTTGAGC GGCGCTCTGG TCGGACAGGG AACCACCCTG 
TATCTCAACG TGACCGACCT CACTTCCTAC AACGTGGGCT ACGACTCTTT CTGCATCAGA 
TGGACTCCTC ATCGAGCTGC CACCTCCTAC AGACTCAAAG TCAACCCTTT CAACCCATCT 
CACCGTGGAG CTCAGGAGGT CACGATCACT GCGGCTGAGA GCCAGTACTG TTTTGACGGC 
CTGACGCCAG ACGCGCTATA CAACGCCACC GTCTACACGC AGACCCCCAA CCGAGAGGGG 
CCCGGAGTCA GTGTCAAAGA GCGCACACTG GTGAAGCCCA CCGTCGCCCC AACACTACCT 
CCAACTCCTC CACCTCCAGC CACCATTCCT TCTGCACTCG AGGTGTGCAA AGGTGCCAAG 
GCTGACCTGG TGTTCCTGAT CGATGGCTCG TGGAGTATCG GAGATGACAG CTTCGCTAAA 
GTCCGGCAGT TTGTGTTCAG CATGATTGGG GCGTTTGATG TGGTCAGCCA TGAGGGCATG 
CAGGTGTCTT TCGTGCAGTA CAGCGACGAC GCCAAAACTG AGTTCAAGCT CAACACCTAC 
CACGACAAGG GAATGGTGCT GTCTGCTCTT CAGATGGTCA ATTATAGAGG AGGAAACACC 
AAAACAGGTG CTGCACTGAA GCATGTTGGA GAAAAAGTGT TCACCTCGGA CAATGGCATG 
AGGAGAACCG TTCCCAAGGT ACTGGTTGTG GTGACCGATG GACGTTCTCA AGATGACGTG 
AAGAAGAGCG CTGCTAAACT ACAGCATGCA GGTTACAGTG TGTTTGTGGT GGGTGTCGCT 
GACATTGACG TTAATGAACT GAGGAACATT GGAAGCAAGC CCAGTGACAG ACACGTCTTT 
ATCGTCGACA ACTTCGATGC TTTCGCCAAG ATCCAAGACA ACCTGATCAC GTTCATCTGT 
GAAACGGCAA CGTCCACATG CCCTTTGATT TACCTCAATG GATTCACATC ACCAGGCTTC 
AGGATGCTGG AAGCTTTCAA CATCACTGAC AAGACCTACG CAGGCATGAA CGGCGTCTCT 
CTGGAAACCG GCTCCTTCAA CAGCTTCATC GCATACAGAC TCCATAAGGA CGCCTTCATT 
TCCCAGCCAA CAAAGGAAAT CCATCCAGAT GGTCTTCCTC CATCCTACAC CCTCATCCTC 
CTCTTCCGTC TGCTCCCGGA CACCGGCAAG GATGCGTTTG ATTTATGGCA GATCTCAGAT 
AAGAACAATA ACCCTGAGGT CGGGCTCACC ATTGACCCCT CCAGCAGAGT GATCTCCTTC 
TACAATAAAG ACACTAGAGG GGAAATTCAG AAAGTCTCCT TTGATAACGA CCACGTGAAG 
AAGATTTTCC ACGGCAGTTT CCACAAGCTT CACATTTCTA TTTCGCCGAA AAATGTCAAA 
CTCAACATCG ACTGCCAAGA GGTGGCTGAG AAACAGATCA AAGCAGCCAA CAACGTCTCC 
ACAGATGGCT ACGAAGTCCT TGGAAAAATG ACAAAGTCTG GCGGTTCAAA GAAGCAGTCG 
GCCACATTCC AGCTCCAGAT GTTTGATATT GTCTGCAGTT TGGGCTGGAC AAGTCGAGAC 
AAATGTTGTG ATATTCCAGC AATGCGAGAT GAAGCCAAGT GTCCTGCTCT TCCTCACTCC 
TGCACATGTG CTCAGGACAG TGTTGGTCCT CCAGGCCCAG CGGGTCCTCA AGGCAGTCCT 
GGTACAAAAG GCCCACGAGG CGAGAGAGGA GAGTCTGGTC CTACGGGTCC AATGGGTCCA 
CGTGGAGAGC AGGGTCTGCC AGGGCAGATG GGGCCACCGG GTCCACAGGG CCCCAATGGA 
CTATCCCTTC CCGGAGAACC AGGGCGACCG GGACCTAAGG GAGACCCCGG TGACTCAGGT 
TTACCTGGAC AGAGAGGCCC GGTTGGCGCT GCAGGTCCAC TCGGCCCTGT TGGACCCGCT 
GGAGCAAGAG GTCCACCAGG AAGTGAAGGC CCATCTGGAC CAAGAGGTCC ATCGGGACCA 
ATGGGACCAC CGGGAGCTCC CGGAATGCCA GGAATCACCG GAAAACCAGG AAAACCCGGA 
GACACTGGCT TTCCTGGACC TGTTGGCATG AAAGGAGAGA AGGGTGAAAG AGGAGACACT 
GCGTCACAGA ATATGATGCG CTCTGTAGCG AGACAAGTCT GCGAACAGCT AGTCAGCAGA 
CAAATGAGCA GAATTGATAT GATGATGAAC CAGATCCCCA GCAACTACAG GAGTAACAGC 
CCCGGCCCCG CTGGACCTCC AGGCCCACCT GGAAACCAGG GACCCCATGG AGAGCCTGGT 
CAGCCCGGAC CCAATGGCTT CCCAGGAAAT CCAGGTTTAC CAGGCTCTCC TGGAGAAAGA 
GGAGTGCCTG GAGAGAAGGG TGAACGAGGC AGCCCCGGCA TTGGCACTCG AGGACAGAGA 
GGATTACCTG GACCTCCTGG GCCACCAGGG CAGTCTCAGA CCGGTCCCCC TGGTCCCTCA 
GGCTCACCCG GGCCCCGAGG ACCACCAGGA CAACATGGTT CTTCTGGAGT CACAGGACCA 
CCAGGACCTC CAGGATACTG TGACTCCTCT CAGTGCGGCA TGCCTTACAA CGGACAAGGC 
TACCCAGGCC CCTACCAGCC CGGGTCTGAA ACCTACGTAG TGCCCATACC AGCCGCGCCA 
TCGGAAGATA TCGAGACACA GTCGCCAGGA TTGCGCTACC AGCGCGGAAA AAGGTCGCTA 
TCAAGAAAGG AAGCCGAAAG AATCAAAACA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291573.1
CDS	178..6030
Translation	MKIRLSLAAAALLTLVLSSVEAQDAQCKTTAQADIVLLVDGSWSIGRLNFKTIRNFIARMVGVFDIGPDRVQIGLAQYSGDPKTEWHLNAHRTRTQLLDAVANLPYKGGNTLTGLALNYILQNNFKPNVGMRPNSRKIGVLVTDGKSQDDIVANSQNLRDQGIELYAIGVKNADENELRTIASDPDDIHMYNVADFSFLLDIVDDLTNNLCNSVKGPGGGPEPPTDLTTSEVTHYSFRATWVAPDGPVEKYRVEYVSTAGGEPQQQMFVDGTVTTVVLEGLTPLTEYIVKVYSVVGEDSSDPLRGIETTLPLSAVRSMNIYKERSTTMNVRWDAATGATGYMLLYKALNATEPSVEQEMRVKGMVTDVQLKKLLPNTAYSVSLFALHGDYASEPLIDQGVTLPLPPSGELRITDVTHSTMQLDWDAAPGAVRKYIITYKPEEGEAKELEVDGDVTSRGLDNLISQTEYDVAVTPVYDSGTGNPMLNQAITDVVPAPQNLRFSMVTQTSFRVHWEHGAPDVSLYRIGWKKKGTTEFQNDILSSEDTTYDLVDLEPDTPYDVSVTAIYPDESESEDLLGTERTLAKLATPPPSGPPQNLQVFDETTTSLTTKWDHAPGRVQNYKITYVPLTGGRSQSTQVGGKKNTVILQKLTPDTPYSITVAAVYASGEARDISGQGKTLPLGSVRNLQVTDPTVSTLNVRWEPAEGSVRQYRIYYQPVAGGAEDMVQVPGSTTSTVLKNLQSDTQYRVTVVPVYAPGEGKRMSETGKTLVRTPAKNIQVYNPTTNTLNVRWEPASGQVQQYRVAYAPLSGIRPSQSVLVPGNTHTALLQQLESDTDYSVSVVALYADGEGSSVSDNGKTLPRSGPRNLRVYDPTTSTLSVGWEHAEGPVIQYRIGYAPTTGDPIEEFTTVPGNRNSVILQNLQPDTPYNINVEAIYQDGPGGRLSGDGKTLDLLGPRNLRVSDEWYTRFRVSWDPAAARVNGYKLVYTPVGTTDTMEAFVGDVTSHHLNNLQPGTTYDLKVYAQYDAGLSGALVGQGTTLYLNVTDLTSYNVGYDSFCIRWTPHRAATSYRLKVNPFNPSHRGAQEVTITAAESQYCFDGLTPDALYNATVYTQTPNREGPGVSVKERTLVKPTVAPTLPPTPPPPATIPSALEVCKGAKADLVFLIDGSWSIGDDSFAKVRQFVFSMIGAFDVVSHEGMQVSFVQYSDDAKTEFKLNTYHDKGMVLSALQMVNYRGGNTKTGAALKHVGEKVFTSDNGMRRTVPKVLVVVTDGRSQDDVKKSAAKLQHAGYSVFVVGVADIDVNELRNIGSKPSDRHVFIVDNFDAFAKIQDNLITFICETATSTCPLIYLNGFTSPGFRMLEAFNITDKTYAGMNGVSLETGSFNSFIAYRLHKDAFISQPTKEIHPDGLPPSYTLILLFRLLPDTGKDAFDLWQISDKNNNPEVGLTIDPSSRVISFYNKDTRGEIQKVSFDNDHVKKIFHGSFHKLHISISPKNVKLNIDCQEVAEKQIKAANNVSTDGYEVLGKMTKSGGSKKQSATFQLQMFDIVCSLGWTSRDKCCDIPAMRDEAKCPALPHSCTCAQDSVGPPGPAGPQGSPGTKGPRGERGESGPTGPMGPRGEQGLPGQMGPPGPQGPNGLSLPGEPGRPGPKGDPGDSGLPGQRGPVGAAGPLGPVGPAGARGPPGSEGPSGPRGPSGPMGPPGAPGMPGITGKPGKPGDTGFPGPVGMKGEKGERGDTASQNMMRSVARQVCEQLVSRQMSRIDMMMNQIPSNYRSNSPGPAGPPGPPGNQGPHGEPGQPGPNGFPGNPGLPGSPGERGVPGEKGERGSPGIGTRGQRGLPGPPGPPGQSQTGPPGPSGSPGPRGPPGQHGSSGVTGPPGPPGYCDSSQCGMPYNGQGYPGPYQPGSETYVVPIPAAPSEDIETQSPGLRYQRGKRSLSRKEAERIKT

Target ORF information:

RefSeq Version	XM_009293298.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type XII, alpha 1a (col12a1a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293298.3

ORF Insert Sequence:

ATGAAGATCA GGTTGTCTTT GGCCGCTGCT GCGCTCTTGA CTCTAGTACT CAGTTCAGTA 
GAGGCTCAAG ATGCCCAGTG TAAAACCACG GCCCAAGCGG ATATCGTCCT GCTTGTTGAT 
GGATCTTGGA GCATTGGTCG CCTGAACTTC AAGACCATAC GCAATTTCAT TGCTCGCATG 
GTGGGAGTCT TCGACATCGG CCCCGATCGT GTCCAGATTG GTCTGGCACA GTACAGCGGA 
GACCCGAAGA CTGAGTGGCA TCTCAATGCT CACAGAACCA GGACTCAGCT GCTGGATGCG 
GTGGCCAATC TACCCTACAA AGGAGGAAAC ACTCTCACCG GCCTTGCTTT GAATTACATC 
CTTCAGAATA ACTTCAAGCC TAACGTTGGC ATGCGTCCAA ACTCCAGAAA GATCGGCGTC 
CTGGTCACTG ATGGCAAGTC CCAAGACGAC ATTGTGGCGA ACTCACAGAA CCTGCGGGAC 
CAAGGGATTG AACTGTACGC CATTGGTGTA AAGAACGCAG ATGAGAATGA GTTGAGAACC 
ATTGCTTCCG ATCCCGATGA CATTCACATG TACAACGTGG CTGATTTCTC CTTCCTGCTG 
GACATTGTGG ATGACCTCAC AAACAACCTG TGCAACAGCG TCAAAGGTCC AGGAGGAGGA 
CCAGAGCCAC CCACTGATCT GACCACCTCA GAGGTCACAC ATTACTCTTT CCGTGCCACT 
TGGGTGGCTC CTGACGGGCC TGTGGAGAAG TACAGAGTGG AGTATGTGAG CACCGCTGGA 
GGAGAACCAC AGCAACAGAT GTTTGTGGAC GGGACGGTGA CCACTGTGGT TTTGGAAGGT 
CTCACTCCGC TGACCGAGTA CATAGTAAAG GTTTACTCAG TGGTGGGAGA GGACAGCAGC 
GACCCACTCA GAGGCATAGA GACCACATTG CCTCTCAGTG CTGTGAGGAG CATGAACATC 
TACAAAGAGC GCTCCACCAC CATGAATGTG AGATGGGACG CAGCCACAGG GGCCACAGGC 
TACATGCTTC TCTACAAGGC CCTTAACGCC ACTGAACCCT CTGTGGAGCA GGAGATGCGA 
GTGAAAGGAA TGGTCACTGA CGTCCAGCTG AAGAAACTTC TCCCCAACAC GGCCTACAGT 
GTTTCGCTCT TCGCCCTGCA CGGCGATTAC GCCAGTGAAC CTCTGATTGA TCAAGGGGTG 
ACCCTGCCTC TTCCTCCATC CGGTGAGTTG AGGATTACCG ATGTGACCCA CAGCACCATG 
CAGCTGGACT GGGACGCTGC GCCCGGCGCC GTCCGAAAGT ACATCATCAC TTACAAGCCA 
GAGGAGGGAG AGGCGAAGGA GCTTGAAGTT GACGGAGATG TAACATCTAG AGGCCTGGAT 
AATCTCATCT CCCAGACTGA ATATGATGTG GCTGTGACTC CGGTTTATGA TTCGGGCACT 
GGAAACCCCA TGCTTAACCA GGCCATTACA GATGTGGTTC CTGCCCCGCA GAACTTGAGG 
TTCTCGATGG TGACCCAGAC CAGTTTTAGG GTTCACTGGG AGCACGGCGC TCCTGATGTC 
TCCCTCTACC GTATTGGCTG GAAAAAGAAA GGAACGACCG AGTTTCAAAA TGATATCCTG 
AGTAGTGAAG ACACTACATA TGATCTGGTG GACCTGGAGC CAGACACACC TTATGATGTT 
TCGGTCACTG CCATTTACCC TGATGAGTCT GAGAGCGAGG ATCTGCTGGG CACTGAACGC 
ACTTTGGCCA AGCTTGCTAC ACCACCTCCC AGTGGTCCTC CTCAAAACCT CCAGGTGTTC 
GACGAGACCA CCACCTCCCT CACGACTAAG TGGGACCACG CGCCTGGACG AGTTCAGAAC 
TACAAAATCA CCTACGTACC GCTTACTGGA GGACGATCAC AGTCGACGCA GGTCGGAGGA 
AAGAAAAACA CAGTGATCCT GCAGAAACTG ACTCCAGACA CTCCTTACTC CATCACTGTT 
GCTGCGGTCT ACGCCAGCGG AGAGGCCAGA GACATTTCTG GCCAAGGAAA GACATTGCCG 
CTGGGCAGTG TCAGGAATCT TCAGGTCACT GACCCCACAG TTAGCACTCT GAATGTGCGC 
TGGGAGCCGG CGGAGGGAAG CGTTCGCCAG TACAGGATCT ACTACCAGCC CGTCGCAGGG 
GGCGCAGAGG ACATGGTCCA GGTTCCTGGA AGCACCACCA GCACCGTTCT GAAGAACCTG 
CAATCTGACA CTCAGTACAG AGTGACGGTG GTGCCAGTCT ACGCTCCTGG AGAGGGCAAG 
CGCATGTCGG AGACCGGAAA AACACTGGTT CGGACCCCTG CCAAGAACAT CCAGGTTTAC 
AACCCCACCA CAAACACCCT GAACGTGCGC TGGGAGCCGG CCTCAGGACA GGTGCAGCAG 
TACCGCGTGG CTTACGCCCC TCTCTCCGGG ATCCGACCCT CTCAGTCGGT GCTGGTTCCA 
GGAAACACCC ACACGGCTCT GCTGCAGCAG CTGGAATCAG ACACTGACTA CTCTGTGAGT 
GTGGTGGCTC TGTATGCTGA TGGAGAGGGC TCGTCTGTGT CTGATAATGG CAAAACACTG 
CCCCGCAGTG GCCCCAGGAA CCTGCGGGTT TATGATCCCA CCACCAGCAC GCTGTCGGTG 
GGCTGGGAAC ATGCAGAAGG GCCTGTGATT CAGTACCGCA TTGGCTACGC ACCCACCACT 
GGAGATCCCA TTGAAGAATT TACAACTGTC CCAGGCAACC GTAACTCGGT CATCCTGCAA 
AACCTGCAGC CAGACACGCC GTACAACATC AACGTAGAAG CCATTTATCA AGATGGCCCT 
GGAGGACGAC TCAGTGGAGA TGGAAAAACA TTGGATCTCC TTGGGCCTCG TAACCTGCGT 
GTTTCAGACG AGTGGTACAC ACGTTTCCGA GTGTCCTGGG ATCCTGCAGC CGCACGGGTG 
AATGGATATA AACTGGTCTA TACACCAGTG GGCACAACCG ACACCATGGA AGCCTTCGTT 
GGAGACGTGA CTTCACACCA CCTGAACAAC CTCCAGCCTG GCACCACATA CGACCTGAAG 
GTTTACGCTC AGTATGATGC GGGCTTGAGC GGCGCTCTGG TCGGACAGGG AACCACCCTG 
TATCTCAACG TGACCGACCT CACTTCCTAC AACGTGGGCT ACGACTCTTT CTGCATCAGA 
TGGACTCCTC ATCGAGCTGC CACCTCCTAC AGACTCAAAG TCAACCCTTT CAACCCATCT 
CACCGTGGAG CTCAGGAGGT CACGATCACT GCGGCTGAGA GCCAGTACTG TTTTGACGGC 
CTGACGCCAG ACGCGCTATA CAACGCCACC GTCTACACGC AGACCCCCAA CCGAGAGGGG 
CCCGGAGTCA GTGTCAAAGA GCGCACACTG GTGAAGCCCA CCGTCGCCCC AACACTACCT 
CCAACTCCTC CACCTCCAGC CACCATTCCT TCTGCACTCG AGGTGTGCAA AGGTGCCAAG 
GCTGACCTGG TGTTCCTGAT CGATGGCTCG TGGAGTATCG GAGATGACAG CTTCGCTAAA 
GTCCGGCAGT TTGTGTTCAG CATGATTGGG GCGTTTGATG TGGTCAGCCA TGAGGGCATG 
CAGGTGTCTT TCGTGCAGTA CAGCGACGAC GCCAAAACTG AGTTCAAGCT CAACACCTAC 
CACGACAAGG GAATGGTGCT GTCTGCTCTT CAGATGGTCA ATTATAGAGG AGGAAACACC 
AAAACAGGTG CTGCACTGAA GCATGTTGGA GAAAAAGTGT TCACCTCGGA CAATGGCATG 
AGGAGAACCG TTCCCAAGGT ACTGGTTGTG GTGACCGATG GACGTTCTCA AGATGACGTG 
AAGAAGAGCG CTGCTAAACT ACAGCATGCA GGTTACAGTG TGTTTGTGGT GGGTGTCGCT 
GACATTGACG TTAATGAACT GAGGAACATT GGAAGCAAGC CCAGTGACAG ACACGTCTTT 
ATCGTCGACA ACTTCGATGC TTTCGCCAAG ATCCAAGACA ACCTGATCAC GTTCATCTGT 
GAAACGGCAA CGTCCACATG CCCTTTGATT TACCTCAATG GATTCACATC ACCAGGCTTC 
AGGATGCTGG AAGCTTTCAA CATCACTGAC AAGACCTACG CAGGCATGAA CGGCGTCTCT 
CTGGAAACCG GCTCCTTCAA CAGCTTCATC GCATACAGAC TCCATAAGGA CGCCTTCATT 
TCCCAGCCAA CAAAGGAAAT CCATCCAGAT GGTCTTCCTC CATCCTACAC CCTCATCCTC 
CTCTTCCGTC TGCTCCCGGA CACCGGCAAG GATGCGTTTG ATTTATGGCA GATCTCAGAT 
AAGAACAATA ACCCTGAGGT CGGGCTCACC ATTGACCCCT CCAGCAGAGT GATCTCCTTC 
TACAATAAAG ACACTAGAGG GGAAATTCAG AAAGTCTCCT TTGATAACGA CCACGTGAAG 
AAGATTTTCC ACGGCAGTTT CCACAAGCTT CACATTTCTA TTTCGCCGAA AAATGTCAAA 
CTCAACATCG ACTGCCAAGA GGTGGCTGAG AAACAGATCA AAGCAGCCAA CAACGTCTCC 
ACAGATGGCT ACGAAGTCCT TGGAAAAATG ACAAAGTCTG GCGGTTCAAA GAAGCAGTCG 
GCCACATTCC AGCTCCAGAT GTTTGATATT GTCTGCAGTT TGGGCTGGAC AAGTCGAGAC 
AAATGTTGTG ATATTCCAGC AATGCGAGAT GAAGCCAAGT GTCCTGCTCT TCCTCACTCC 
TGCACATGTG CTCAGGACAG TGTTGGTCCT CCAGGCCCAG CGGGTCCTCA AGGCAGTCCT 
GGTACAAAAG GCCCACGAGG CGAGAGAGGA GAGTCTGGTC CTACGGGTCC AATGGGTCCA 
CGTGGAGAGC AGGGTCTGCC AGGGCAGATG GGGCCACCGG GTCCACAGGG CCCCAATGGA 
CTATCCCTTC CCGGAGAACC AGGGCGACCG GGACCTAAGG GAGACCCCGG TGACTCAGGT 
TTACCTGGAC AGAGAGGCCC GGTTGGCGCT GCAGGTCCAC TCGGCCCTGT TGGACCCGCT 
GGAGCAAGAG GTCCACCAGG AAGTGAAGGC CCATCTGGAC CAAGAGGTCC ATCGGGACCA 
ATGGGACCAC CGGGAGCTCC CGGAATGCCA GGAATCACCG GAAAACCAGG AAAACCCGGA 
GACACTGGCT TTCCTGGACC TGTTGGCATG AAAGGAGAGA AGGGTGAAAG AGGAGACACT 
GCGTCACAGA ATATGATGCG CTCTGTAGCG AGACAAGTCT GCGAACAGCT AGTCAGCAGA 
CAAATGAGCA GAATTGATAT GATGATGAAC CAGATCCCCA GCAACTACAG GAGTAACAGC 
CCCGGCCCCG CTGGACCTCC AGGCCCACCT GGAAACCAGG GACCCCATGG AGAGCCTGGT 
CAGCCCGGAC CCAATGGCTT CCCAGGAAAT CCAGGTTTAC CAGGCTCTCC TGGAGAAAGA 
GGAGTGCCTG GAGAGAAGGG TGAACGAGGC AGCCCCGGCA TTGGCACTCG AGGACAGAGA 
GGATTACCTG GACCTCCTGG GCCACCAGGG CAGTCTCAGA CCGGTCCCCC TGGTCCCTCA 
GGCTCACCCG GGCCCCGAGG ACCACCAGGA CAACATGGTT CTTCTGGAGT CACAGGACCA 
CCAGGACCTC CAGGATACTG TGACTCCTCT CAGTGCGGCA TGCCTTACAA CGGACAAGGC 
TACCCAGGCC CCTACCAGCC CGGGTCTGAA ACCTACGTAG TGCCCATACC AGCCGCGCCA 
TCGGAAGATA TCGAGACACA GTCGCCAGGA TTGCGCTACC AGCGCGGAAA AAGGTCGCTA 
TCAAGAAAGG AAGCCGAAAG AATCAAAACA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL12A1A ( XM_009293298.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009291573.1 Danio rerio collagen, type XII, alpha 1a (col12a1a), transcript variant X4, mRNA.

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CloneID	ODa48774
Clone ID Related Accession (Same CDS sequence)	XM_009293298.3 , XM_009293298.2
Accession Version	XM_009293298.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	collagen alpha-1(XII) chain isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293298.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATCA GGTTGTCTTT GGCCGCTGCT GCGCTCTTGA CTCTAGTACT CAGTTCAGTA 
GAGGCTCAAG ATGCCCAGTG TAAAACCACG GCCCAAGCGG ATATCGTCCT GCTTGTTGAT 
GGATCTTGGA GCATTGGTCG CCTGAACTTC AAGACCATAC GCAATTTCAT TGCTCGCATG 
GTGGGAGTCT TCGACATCGG CCCCGATCGT GTCCAGATTG GTCTGGCACA GTACAGCGGA 
GACCCGAAGA CTGAGTGGCA TCTCAATGCT CACAGAACCA GGACTCAGCT GCTGGATGCG 
GTGGCCAATC TACCCTACAA AGGAGGAAAC ACTCTCACCG GCCTTGCTTT GAATTACATC 
CTTCAGAATA ACTTCAAGCC TAACGTTGGC ATGCGTCCAA ACTCCAGAAA GATCGGCGTC 
CTGGTCACTG ATGGCAAGTC CCAAGACGAC ATTGTGGCGA ACTCACAGAA CCTGCGGGAC 
CAAGGGATTG AACTGTACGC CATTGGTGTA AAGAACGCAG ATGAGAATGA GTTGAGAACC 
ATTGCTTCCG ATCCCGATGA CATTCACATG TACAACGTGG CTGATTTCTC CTTCCTGCTG 
GACATTGTGG ATGACCTCAC AAACAACCTG TGCAACAGCG TCAAAGGTCC AGGAGGAGGA 
CCAGAGCCAC CCACTGATCT GACCACCTCA GAGGTCACAC ATTACTCTTT CCGTGCCACT 
TGGGTGGCTC CTGACGGGCC TGTGGAGAAG TACAGAGTGG AGTATGTGAG CACCGCTGGA 
GGAGAACCAC AGCAACAGAT GTTTGTGGAC GGGACGGTGA CCACTGTGGT TTTGGAAGGT 
CTCACTCCGC TGACCGAGTA CATAGTAAAG GTTTACTCAG TGGTGGGAGA GGACAGCAGC 
GACCCACTCA GAGGCATAGA GACCACATTG CCTCTCAGTG CTGTGAGGAG CATGAACATC 
TACAAAGAGC GCTCCACCAC CATGAATGTG AGATGGGACG CAGCCACAGG GGCCACAGGC 
TACATGCTTC TCTACAAGGC CCTTAACGCC ACTGAACCCT CTGTGGAGCA GGAGATGCGA 
GTGAAAGGAA TGGTCACTGA CGTCCAGCTG AAGAAACTTC TCCCCAACAC GGCCTACAGT 
GTTTCGCTCT TCGCCCTGCA CGGCGATTAC GCCAGTGAAC CTCTGATTGA TCAAGGGGTG 
ACCCTGCCTC TTCCTCCATC CGGTGAGTTG AGGATTACCG ATGTGACCCA CAGCACCATG 
CAGCTGGACT GGGACGCTGC GCCCGGCGCC GTCCGAAAGT ACATCATCAC TTACAAGCCA 
GAGGAGGGAG AGGCGAAGGA GCTTGAAGTT GACGGAGATG TAACATCTAG AGGCCTGGAT 
AATCTCATCT CCCAGACTGA ATATGATGTG GCTGTGACTC CGGTTTATGA TTCGGGCACT 
GGAAACCCCA TGCTTAACCA GGCCATTACA GATGTGGTTC CTGCCCCGCA GAACTTGAGG 
TTCTCGATGG TGACCCAGAC CAGTTTTAGG GTTCACTGGG AGCACGGCGC TCCTGATGTC 
TCCCTCTACC GTATTGGCTG GAAAAAGAAA GGAACGACCG AGTTTCAAAA TGATATCCTG 
AGTAGTGAAG ACACTACATA TGATCTGGTG GACCTGGAGC CAGACACACC TTATGATGTT 
TCGGTCACTG CCATTTACCC TGATGAGTCT GAGAGCGAGG ATCTGCTGGG CACTGAACGC 
ACTTTGGCCA AGCTTGCTAC ACCACCTCCC AGTGGTCCTC CTCAAAACCT CCAGGTGTTC 
GACGAGACCA CCACCTCCCT CACGACTAAG TGGGACCACG CGCCTGGACG AGTTCAGAAC 
TACAAAATCA CCTACGTACC GCTTACTGGA GGACGATCAC AGTCGACGCA GGTCGGAGGA 
AAGAAAAACA CAGTGATCCT GCAGAAACTG ACTCCAGACA CTCCTTACTC CATCACTGTT 
GCTGCGGTCT ACGCCAGCGG AGAGGCCAGA GACATTTCTG GCCAAGGAAA GACATTGCCG 
CTGGGCAGTG TCAGGAATCT TCAGGTCACT GACCCCACAG TTAGCACTCT GAATGTGCGC 
TGGGAGCCGG CGGAGGGAAG CGTTCGCCAG TACAGGATCT ACTACCAGCC CGTCGCAGGG 
GGCGCAGAGG ACATGGTCCA GGTTCCTGGA AGCACCACCA GCACCGTTCT GAAGAACCTG 
CAATCTGACA CTCAGTACAG AGTGACGGTG GTGCCAGTCT ACGCTCCTGG AGAGGGCAAG 
CGCATGTCGG AGACCGGAAA AACACTGGTT CGGACCCCTG CCAAGAACAT CCAGGTTTAC 
AACCCCACCA CAAACACCCT GAACGTGCGC TGGGAGCCGG CCTCAGGACA GGTGCAGCAG 
TACCGCGTGG CTTACGCCCC TCTCTCCGGG ATCCGACCCT CTCAGTCGGT GCTGGTTCCA 
GGAAACACCC ACACGGCTCT GCTGCAGCAG CTGGAATCAG ACACTGACTA CTCTGTGAGT 
GTGGTGGCTC TGTATGCTGA TGGAGAGGGC TCGTCTGTGT CTGATAATGG CAAAACACTG 
CCCCGCAGTG GCCCCAGGAA CCTGCGGGTT TATGATCCCA CCACCAGCAC GCTGTCGGTG 
GGCTGGGAAC ATGCAGAAGG GCCTGTGATT CAGTACCGCA TTGGCTACGC ACCCACCACT 
GGAGATCCCA TTGAAGAATT TACAACTGTC CCAGGCAACC GTAACTCGGT CATCCTGCAA 
AACCTGCAGC CAGACACGCC GTACAACATC AACGTAGAAG CCATTTATCA AGATGGCCCT 
GGAGGACGAC TCAGTGGAGA TGGAAAAACA TTGGATCTCC TTGGGCCTCG TAACCTGCGT 
GTTTCAGACG AGTGGTACAC ACGTTTCCGA GTGTCCTGGG ATCCTGCAGC CGCACGGGTG 
AATGGATATA AACTGGTCTA TACACCAGTG GGCACAACCG ACACCATGGA AGCCTTCGTT 
GGAGACGTGA CTTCACACCA CCTGAACAAC CTCCAGCCTG GCACCACATA CGACCTGAAG 
GTTTACGCTC AGTATGATGC GGGCTTGAGC GGCGCTCTGG TCGGACAGGG AACCACCCTG 
TATCTCAACG TGACCGACCT CACTTCCTAC AACGTGGGCT ACGACTCTTT CTGCATCAGA 
TGGACTCCTC ATCGAGCTGC CACCTCCTAC AGACTCAAAG TCAACCCTTT CAACCCATCT 
CACCGTGGAG CTCAGGAGGT CACGATCACT GCGGCTGAGA GCCAGTACTG TTTTGACGGC 
CTGACGCCAG ACGCGCTATA CAACGCCACC GTCTACACGC AGACCCCCAA CCGAGAGGGG 
CCCGGAGTCA GTGTCAAAGA GCGCACACTG GTGAAGCCCA CCGTCGCCCC AACACTACCT 
CCAACTCCTC CACCTCCAGC CACCATTCCT TCTGCACTCG AGGTGTGCAA AGGTGCCAAG 
GCTGACCTGG TGTTCCTGAT CGATGGCTCG TGGAGTATCG GAGATGACAG CTTCGCTAAA 
GTCCGGCAGT TTGTGTTCAG CATGATTGGG GCGTTTGATG TGGTCAGCCA TGAGGGCATG 
CAGGTGTCTT TCGTGCAGTA CAGCGACGAC GCCAAAACTG AGTTCAAGCT CAACACCTAC 
CACGACAAGG GAATGGTGCT GTCTGCTCTT CAGATGGTCA ATTATAGAGG AGGAAACACC 
AAAACAGGTG CTGCACTGAA GCATGTTGGA GAAAAAGTGT TCACCTCGGA CAATGGCATG 
AGGAGAACCG TTCCCAAGGT ACTGGTTGTG GTGACCGATG GACGTTCTCA AGATGACGTG 
AAGAAGAGCG CTGCTAAACT ACAGCATGCA GGTTACAGTG TGTTTGTGGT GGGTGTCGCT 
GACATTGACG TTAATGAACT GAGGAACATT GGAAGCAAGC CCAGTGACAG ACACGTCTTT 
ATCGTCGACA ACTTCGATGC TTTCGCCAAG ATCCAAGACA ACCTGATCAC GTTCATCTGT 
GAAACGGCAA CGTCCACATG CCCTTTGATT TACCTCAATG GATTCACATC ACCAGGCTTC 
AGGATGCTGG AAGCTTTCAA CATCACTGAC AAGACCTACG CAGGCATGAA CGGCGTCTCT 
CTGGAAACCG GCTCCTTCAA CAGCTTCATC GCATACAGAC TCCATAAGGA CGCCTTCATT 
TCCCAGCCAA CAAAGGAAAT CCATCCAGAT GGTCTTCCTC CATCCTACAC CCTCATCCTC 
CTCTTCCGTC TGCTCCCGGA CACCGGCAAG GATGCGTTTG ATTTATGGCA GATCTCAGAT 
AAGAACAATA ACCCTGAGGT CGGGCTCACC ATTGACCCCT CCAGCAGAGT GATCTCCTTC 
TACAATAAAG ACACTAGAGG GGAAATTCAG AAAGTCTCCT TTGATAACGA CCACGTGAAG 
AAGATTTTCC ACGGCAGTTT CCACAAGCTT CACATTTCTA TTTCGCCGAA AAATGTCAAA 
CTCAACATCG ACTGCCAAGA GGTGGCTGAG AAACAGATCA AAGCAGCCAA CAACGTCTCC 
ACAGATGGCT ACGAAGTCCT TGGAAAAATG ACAAAGTCTG GCGGTTCAAA GAAGCAGTCG 
GCCACATTCC AGCTCCAGAT GTTTGATATT GTCTGCAGTT TGGGCTGGAC AAGTCGAGAC 
AAATGTTGTG ATATTCCAGC AATGCGAGAT GAAGCCAAGT GTCCTGCTCT TCCTCACTCC 
TGCACATGTG CTCAGGACAG TGTTGGTCCT CCAGGCCCAG CGGGTCCTCA AGGCAGTCCT 
GGTACAAAAG GCCCACGAGG CGAGAGAGGA GAGTCTGGTC CTACGGGTCC AATGGGTCCA 
CGTGGAGAGC AGGGTCTGCC AGGGCAGATG GGGCCACCGG GTCCACAGGG CCCCAATGGA 
CTATCCCTTC CCGGAGAACC AGGGCGACCG GGACCTAAGG GAGACCCCGG TGACTCAGGT 
TTACCTGGAC AGAGAGGCCC GGTTGGCGCT GCAGGTCCAC TCGGCCCTGT TGGACCCGCT 
GGAGCAAGAG GTCCACCAGG AAGTGAAGGC CCATCTGGAC CAAGAGGTCC ATCGGGACCA 
ATGGGACCAC CGGGAGCTCC CGGAATGCCA GGAATCACCG GAAAACCAGG AAAACCCGGA 
GACACTGGCT TTCCTGGACC TGTTGGCATG AAAGGAGAGA AGGGTGAAAG AGGAGACACT 
GCGTCACAGA ATATGATGCG CTCTGTAGCG AGACAAGTCT GCGAACAGCT AGTCAGCAGA 
CAAATGAGCA GAATTGATAT GATGATGAAC CAGATCCCCA GCAACTACAG GAGTAACAGC 
CCCGGCCCCG CTGGACCTCC AGGCCCACCT GGAAACCAGG GACCCCATGG AGAGCCTGGT 
CAGCCCGGAC CCAATGGCTT CCCAGGAAAT CCAGGTTTAC CAGGCTCTCC TGGAGAAAGA 
GGAGTGCCTG GAGAGAAGGG TGAACGAGGC AGCCCCGGCA TTGGCACTCG AGGACAGAGA 
GGATTACCTG GACCTCCTGG GCCACCAGGG CAGTCTCAGA CCGGTCCCCC TGGTCCCTCA 
GGCTCACCCG GGCCCCGAGG ACCACCAGGA CAACATGGTT CTTCTGGAGT CACAGGACCA 
CCAGGACCTC CAGGATACTG TGACTCCTCT CAGTGCGGCA TGCCTTACAA CGGACAAGGC 
TACCCAGGCC CCTACCAGCC CGGGTCTGAA ACCTACGTAG TGCCCATACC AGCCGCGCCA 
TCGGAAGATA TCGAGACACA GTCGCCAGGA TTGCGCTACC AGCGCGGAAA AAGGTCGCTA 
TCAAGAAAGG AAGCCGAAAG AATCAAAACA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291573.1
CDS	137..5989
Translation	MKIRLSLAAAALLTLVLSSVEAQDAQCKTTAQADIVLLVDGSWSIGRLNFKTIRNFIARMVGVFDIGPDRVQIGLAQYSGDPKTEWHLNAHRTRTQLLDAVANLPYKGGNTLTGLALNYILQNNFKPNVGMRPNSRKIGVLVTDGKSQDDIVANSQNLRDQGIELYAIGVKNADENELRTIASDPDDIHMYNVADFSFLLDIVDDLTNNLCNSVKGPGGGPEPPTDLTTSEVTHYSFRATWVAPDGPVEKYRVEYVSTAGGEPQQQMFVDGTVTTVVLEGLTPLTEYIVKVYSVVGEDSSDPLRGIETTLPLSAVRSMNIYKERSTTMNVRWDAATGATGYMLLYKALNATEPSVEQEMRVKGMVTDVQLKKLLPNTAYSVSLFALHGDYASEPLIDQGVTLPLPPSGELRITDVTHSTMQLDWDAAPGAVRKYIITYKPEEGEAKELEVDGDVTSRGLDNLISQTEYDVAVTPVYDSGTGNPMLNQAITDVVPAPQNLRFSMVTQTSFRVHWEHGAPDVSLYRIGWKKKGTTEFQNDILSSEDTTYDLVDLEPDTPYDVSVTAIYPDESESEDLLGTERTLAKLATPPPSGPPQNLQVFDETTTSLTTKWDHAPGRVQNYKITYVPLTGGRSQSTQVGGKKNTVILQKLTPDTPYSITVAAVYASGEARDISGQGKTLPLGSVRNLQVTDPTVSTLNVRWEPAEGSVRQYRIYYQPVAGGAEDMVQVPGSTTSTVLKNLQSDTQYRVTVVPVYAPGEGKRMSETGKTLVRTPAKNIQVYNPTTNTLNVRWEPASGQVQQYRVAYAPLSGIRPSQSVLVPGNTHTALLQQLESDTDYSVSVVALYADGEGSSVSDNGKTLPRSGPRNLRVYDPTTSTLSVGWEHAEGPVIQYRIGYAPTTGDPIEEFTTVPGNRNSVILQNLQPDTPYNINVEAIYQDGPGGRLSGDGKTLDLLGPRNLRVSDEWYTRFRVSWDPAAARVNGYKLVYTPVGTTDTMEAFVGDVTSHHLNNLQPGTTYDLKVYAQYDAGLSGALVGQGTTLYLNVTDLTSYNVGYDSFCIRWTPHRAATSYRLKVNPFNPSHRGAQEVTITAAESQYCFDGLTPDALYNATVYTQTPNREGPGVSVKERTLVKPTVAPTLPPTPPPPATIPSALEVCKGAKADLVFLIDGSWSIGDDSFAKVRQFVFSMIGAFDVVSHEGMQVSFVQYSDDAKTEFKLNTYHDKGMVLSALQMVNYRGGNTKTGAALKHVGEKVFTSDNGMRRTVPKVLVVVTDGRSQDDVKKSAAKLQHAGYSVFVVGVADIDVNELRNIGSKPSDRHVFIVDNFDAFAKIQDNLITFICETATSTCPLIYLNGFTSPGFRMLEAFNITDKTYAGMNGVSLETGSFNSFIAYRLHKDAFISQPTKEIHPDGLPPSYTLILLFRLLPDTGKDAFDLWQISDKNNNPEVGLTIDPSSRVISFYNKDTRGEIQKVSFDNDHVKKIFHGSFHKLHISISPKNVKLNIDCQEVAEKQIKAANNVSTDGYEVLGKMTKSGGSKKQSATFQLQMFDIVCSLGWTSRDKCCDIPAMRDEAKCPALPHSCTCAQDSVGPPGPAGPQGSPGTKGPRGERGESGPTGPMGPRGEQGLPGQMGPPGPQGPNGLSLPGEPGRPGPKGDPGDSGLPGQRGPVGAAGPLGPVGPAGARGPPGSEGPSGPRGPSGPMGPPGAPGMPGITGKPGKPGDTGFPGPVGMKGEKGERGDTASQNMMRSVARQVCEQLVSRQMSRIDMMMNQIPSNYRSNSPGPAGPPGPPGNQGPHGEPGQPGPNGFPGNPGLPGSPGERGVPGEKGERGSPGIGTRGQRGLPGPPGPPGQSQTGPPGPSGSPGPRGPPGQHGSSGVTGPPGPPGYCDSSQCGMPYNGQGYPGPYQPGSETYVVPIPAAPSEDIETQSPGLRYQRGKRSLSRKEAERIKT

Target ORF information:

RefSeq Version	XM_009293298.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio collagen, type XII, alpha 1a (col12a1a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293298.2

ORF Insert Sequence:

ATGAAGATCA GGTTGTCTTT GGCCGCTGCT GCGCTCTTGA CTCTAGTACT CAGTTCAGTA 
GAGGCTCAAG ATGCCCAGTG TAAAACCACG GCCCAAGCGG ATATCGTCCT GCTTGTTGAT 
GGATCTTGGA GCATTGGTCG CCTGAACTTC AAGACCATAC GCAATTTCAT TGCTCGCATG 
GTGGGAGTCT TCGACATCGG CCCCGATCGT GTCCAGATTG GTCTGGCACA GTACAGCGGA 
GACCCGAAGA CTGAGTGGCA TCTCAATGCT CACAGAACCA GGACTCAGCT GCTGGATGCG 
GTGGCCAATC TACCCTACAA AGGAGGAAAC ACTCTCACCG GCCTTGCTTT GAATTACATC 
CTTCAGAATA ACTTCAAGCC TAACGTTGGC ATGCGTCCAA ACTCCAGAAA GATCGGCGTC 
CTGGTCACTG ATGGCAAGTC CCAAGACGAC ATTGTGGCGA ACTCACAGAA CCTGCGGGAC 
CAAGGGATTG AACTGTACGC CATTGGTGTA AAGAACGCAG ATGAGAATGA GTTGAGAACC 
ATTGCTTCCG ATCCCGATGA CATTCACATG TACAACGTGG CTGATTTCTC CTTCCTGCTG 
GACATTGTGG ATGACCTCAC AAACAACCTG TGCAACAGCG TCAAAGGTCC AGGAGGAGGA 
CCAGAGCCAC CCACTGATCT GACCACCTCA GAGGTCACAC ATTACTCTTT CCGTGCCACT 
TGGGTGGCTC CTGACGGGCC TGTGGAGAAG TACAGAGTGG AGTATGTGAG CACCGCTGGA 
GGAGAACCAC AGCAACAGAT GTTTGTGGAC GGGACGGTGA CCACTGTGGT TTTGGAAGGT 
CTCACTCCGC TGACCGAGTA CATAGTAAAG GTTTACTCAG TGGTGGGAGA GGACAGCAGC 
GACCCACTCA GAGGCATAGA GACCACATTG CCTCTCAGTG CTGTGAGGAG CATGAACATC 
TACAAAGAGC GCTCCACCAC CATGAATGTG AGATGGGACG CAGCCACAGG GGCCACAGGC 
TACATGCTTC TCTACAAGGC CCTTAACGCC ACTGAACCCT CTGTGGAGCA GGAGATGCGA 
GTGAAAGGAA TGGTCACTGA CGTCCAGCTG AAGAAACTTC TCCCCAACAC GGCCTACAGT 
GTTTCGCTCT TCGCCCTGCA CGGCGATTAC GCCAGTGAAC CTCTGATTGA TCAAGGGGTG 
ACCCTGCCTC TTCCTCCATC CGGTGAGTTG AGGATTACCG ATGTGACCCA CAGCACCATG 
CAGCTGGACT GGGACGCTGC GCCCGGCGCC GTCCGAAAGT ACATCATCAC TTACAAGCCA 
GAGGAGGGAG AGGCGAAGGA GCTTGAAGTT GACGGAGATG TAACATCTAG AGGCCTGGAT 
AATCTCATCT CCCAGACTGA ATATGATGTG GCTGTGACTC CGGTTTATGA TTCGGGCACT 
GGAAACCCCA TGCTTAACCA GGCCATTACA GATGTGGTTC CTGCCCCGCA GAACTTGAGG 
TTCTCGATGG TGACCCAGAC CAGTTTTAGG GTTCACTGGG AGCACGGCGC TCCTGATGTC 
TCCCTCTACC GTATTGGCTG GAAAAAGAAA GGAACGACCG AGTTTCAAAA TGATATCCTG 
AGTAGTGAAG ACACTACATA TGATCTGGTG GACCTGGAGC CAGACACACC TTATGATGTT 
TCGGTCACTG CCATTTACCC TGATGAGTCT GAGAGCGAGG ATCTGCTGGG CACTGAACGC 
ACTTTGGCCA AGCTTGCTAC ACCACCTCCC AGTGGTCCTC CTCAAAACCT CCAGGTGTTC 
GACGAGACCA CCACCTCCCT CACGACTAAG TGGGACCACG CGCCTGGACG AGTTCAGAAC 
TACAAAATCA CCTACGTACC GCTTACTGGA GGACGATCAC AGTCGACGCA GGTCGGAGGA 
AAGAAAAACA CAGTGATCCT GCAGAAACTG ACTCCAGACA CTCCTTACTC CATCACTGTT 
GCTGCGGTCT ACGCCAGCGG AGAGGCCAGA GACATTTCTG GCCAAGGAAA GACATTGCCG 
CTGGGCAGTG TCAGGAATCT TCAGGTCACT GACCCCACAG TTAGCACTCT GAATGTGCGC 
TGGGAGCCGG CGGAGGGAAG CGTTCGCCAG TACAGGATCT ACTACCAGCC CGTCGCAGGG 
GGCGCAGAGG ACATGGTCCA GGTTCCTGGA AGCACCACCA GCACCGTTCT GAAGAACCTG 
CAATCTGACA CTCAGTACAG AGTGACGGTG GTGCCAGTCT ACGCTCCTGG AGAGGGCAAG 
CGCATGTCGG AGACCGGAAA AACACTGGTT CGGACCCCTG CCAAGAACAT CCAGGTTTAC 
AACCCCACCA CAAACACCCT GAACGTGCGC TGGGAGCCGG CCTCAGGACA GGTGCAGCAG 
TACCGCGTGG CTTACGCCCC TCTCTCCGGG ATCCGACCCT CTCAGTCGGT GCTGGTTCCA 
GGAAACACCC ACACGGCTCT GCTGCAGCAG CTGGAATCAG ACACTGACTA CTCTGTGAGT 
GTGGTGGCTC TGTATGCTGA TGGAGAGGGC TCGTCTGTGT CTGATAATGG CAAAACACTG 
CCCCGCAGTG GCCCCAGGAA CCTGCGGGTT TATGATCCCA CCACCAGCAC GCTGTCGGTG 
GGCTGGGAAC ATGCAGAAGG GCCTGTGATT CAGTACCGCA TTGGCTACGC ACCCACCACT 
GGAGATCCCA TTGAAGAATT TACAACTGTC CCAGGCAACC GTAACTCGGT CATCCTGCAA 
AACCTGCAGC CAGACACGCC GTACAACATC AACGTAGAAG CCATTTATCA AGATGGCCCT 
GGAGGACGAC TCAGTGGAGA TGGAAAAACA TTGGATCTCC TTGGGCCTCG TAACCTGCGT 
GTTTCAGACG AGTGGTACAC ACGTTTCCGA GTGTCCTGGG ATCCTGCAGC CGCACGGGTG 
AATGGATATA AACTGGTCTA TACACCAGTG GGCACAACCG ACACCATGGA AGCCTTCGTT 
GGAGACGTGA CTTCACACCA CCTGAACAAC CTCCAGCCTG GCACCACATA CGACCTGAAG 
GTTTACGCTC AGTATGATGC GGGCTTGAGC GGCGCTCTGG TCGGACAGGG AACCACCCTG 
TATCTCAACG TGACCGACCT CACTTCCTAC AACGTGGGCT ACGACTCTTT CTGCATCAGA 
TGGACTCCTC ATCGAGCTGC CACCTCCTAC AGACTCAAAG TCAACCCTTT CAACCCATCT 
CACCGTGGAG CTCAGGAGGT CACGATCACT GCGGCTGAGA GCCAGTACTG TTTTGACGGC 
CTGACGCCAG ACGCGCTATA CAACGCCACC GTCTACACGC AGACCCCCAA CCGAGAGGGG 
CCCGGAGTCA GTGTCAAAGA GCGCACACTG GTGAAGCCCA CCGTCGCCCC AACACTACCT 
CCAACTCCTC CACCTCCAGC CACCATTCCT TCTGCACTCG AGGTGTGCAA AGGTGCCAAG 
GCTGACCTGG TGTTCCTGAT CGATGGCTCG TGGAGTATCG GAGATGACAG CTTCGCTAAA 
GTCCGGCAGT TTGTGTTCAG CATGATTGGG GCGTTTGATG TGGTCAGCCA TGAGGGCATG 
CAGGTGTCTT TCGTGCAGTA CAGCGACGAC GCCAAAACTG AGTTCAAGCT CAACACCTAC 
CACGACAAGG GAATGGTGCT GTCTGCTCTT CAGATGGTCA ATTATAGAGG AGGAAACACC 
AAAACAGGTG CTGCACTGAA GCATGTTGGA GAAAAAGTGT TCACCTCGGA CAATGGCATG 
AGGAGAACCG TTCCCAAGGT ACTGGTTGTG GTGACCGATG GACGTTCTCA AGATGACGTG 
AAGAAGAGCG CTGCTAAACT ACAGCATGCA GGTTACAGTG TGTTTGTGGT GGGTGTCGCT 
GACATTGACG TTAATGAACT GAGGAACATT GGAAGCAAGC CCAGTGACAG ACACGTCTTT 
ATCGTCGACA ACTTCGATGC TTTCGCCAAG ATCCAAGACA ACCTGATCAC GTTCATCTGT 
GAAACGGCAA CGTCCACATG CCCTTTGATT TACCTCAATG GATTCACATC ACCAGGCTTC 
AGGATGCTGG AAGCTTTCAA CATCACTGAC AAGACCTACG CAGGCATGAA CGGCGTCTCT 
CTGGAAACCG GCTCCTTCAA CAGCTTCATC GCATACAGAC TCCATAAGGA CGCCTTCATT 
TCCCAGCCAA CAAAGGAAAT CCATCCAGAT GGTCTTCCTC CATCCTACAC CCTCATCCTC 
CTCTTCCGTC TGCTCCCGGA CACCGGCAAG GATGCGTTTG ATTTATGGCA GATCTCAGAT 
AAGAACAATA ACCCTGAGGT CGGGCTCACC ATTGACCCCT CCAGCAGAGT GATCTCCTTC 
TACAATAAAG ACACTAGAGG GGAAATTCAG AAAGTCTCCT TTGATAACGA CCACGTGAAG 
AAGATTTTCC ACGGCAGTTT CCACAAGCTT CACATTTCTA TTTCGCCGAA AAATGTCAAA 
CTCAACATCG ACTGCCAAGA GGTGGCTGAG AAACAGATCA AAGCAGCCAA CAACGTCTCC 
ACAGATGGCT ACGAAGTCCT TGGAAAAATG ACAAAGTCTG GCGGTTCAAA GAAGCAGTCG 
GCCACATTCC AGCTCCAGAT GTTTGATATT GTCTGCAGTT TGGGCTGGAC AAGTCGAGAC 
AAATGTTGTG ATATTCCAGC AATGCGAGAT GAAGCCAAGT GTCCTGCTCT TCCTCACTCC 
TGCACATGTG CTCAGGACAG TGTTGGTCCT CCAGGCCCAG CGGGTCCTCA AGGCAGTCCT 
GGTACAAAAG GCCCACGAGG CGAGAGAGGA GAGTCTGGTC CTACGGGTCC AATGGGTCCA 
CGTGGAGAGC AGGGTCTGCC AGGGCAGATG GGGCCACCGG GTCCACAGGG CCCCAATGGA 
CTATCCCTTC CCGGAGAACC AGGGCGACCG GGACCTAAGG GAGACCCCGG TGACTCAGGT 
TTACCTGGAC AGAGAGGCCC GGTTGGCGCT GCAGGTCCAC TCGGCCCTGT TGGACCCGCT 
GGAGCAAGAG GTCCACCAGG AAGTGAAGGC CCATCTGGAC CAAGAGGTCC ATCGGGACCA 
ATGGGACCAC CGGGAGCTCC CGGAATGCCA GGAATCACCG GAAAACCAGG AAAACCCGGA 
GACACTGGCT TTCCTGGACC TGTTGGCATG AAAGGAGAGA AGGGTGAAAG AGGAGACACT 
GCGTCACAGA ATATGATGCG CTCTGTAGCG AGACAAGTCT GCGAACAGCT AGTCAGCAGA 
CAAATGAGCA GAATTGATAT GATGATGAAC CAGATCCCCA GCAACTACAG GAGTAACAGC 
CCCGGCCCCG CTGGACCTCC AGGCCCACCT GGAAACCAGG GACCCCATGG AGAGCCTGGT 
CAGCCCGGAC CCAATGGCTT CCCAGGAAAT CCAGGTTTAC CAGGCTCTCC TGGAGAAAGA 
GGAGTGCCTG GAGAGAAGGG TGAACGAGGC AGCCCCGGCA TTGGCACTCG AGGACAGAGA 
GGATTACCTG GACCTCCTGG GCCACCAGGG CAGTCTCAGA CCGGTCCCCC TGGTCCCTCA 
GGCTCACCCG GGCCCCGAGG ACCACCAGGA CAACATGGTT CTTCTGGAGT CACAGGACCA 
CCAGGACCTC CAGGATACTG TGACTCCTCT CAGTGCGGCA TGCCTTACAA CGGACAAGGC 
TACCCAGGCC CCTACCAGCC CGGGTCTGAA ACCTACGTAG TGCCCATACC AGCCGCGCCA 
TCGGAAGATA TCGAGACACA GTCGCCAGGA TTGCGCTACC AGCGCGGAAA AAGGTCGCTA 
TCAAGAAAGG AAGCCGAAAG AATCAAAACA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL12A1A ( XM_002665259.6 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_002665305.3 Danio rerio collagen, type XII, alpha 1a (col12a1a), transcript variant X2, mRNA.

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CloneID	ODa22912
Clone ID Related Accession (Same CDS sequence)	XM_002665259.6 , XM_002665259.5
Accession Version	XM_002665259.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	collagen alpha-1(XII) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_002665259.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATCA GGTTGTCTTT GGCCGCTGCT GCGCTCTTGA CTCTAGTACT CAGTTCAGTA 
GAGGCTCAAG TGGAGCCTCC GTCAGACTTA AAATTTAAAA TCCTGAATGA AAACACAGTG 
CAAATGTCAT GGAGGAAGCC CTCGTCTCAG ATACAGGGCT ACAGAATACA GGTCACGTCA 
GATACAGATG ATTCTAAAGA ATTGAGTCTT CCTGCCTCGT CCACAAGCAC ATCAATTACA 
GATCTAACTC CAGATGTAGA CTATTCAGTG TCAATCAATT CATATGATGG TGCTGAAGAA 
AGTATCCCTA TCCTTGGACA AATCACAATT CAATCCAGCA ACACAAGTGG ACCAAGGAGG 
CCACAGTCAG AGGCTGTCAA GTGTTCAGTG AGTGCCGTTG CGGATCTGGT TTTCCTGGTG 
GACGGCTCTT GGAGTGTGGG AAGAGAAAAC TTTAGATTTA TCCGGAGTTT CATTGGGGCC 
ATGGCCGGGG CCTTCGACAT TGAAGAGGAT AAGACCAGAG TGGCTGTGGT TCAGTACAGC 
TCGGACACCA GGACAGAGTT CAGTCTAAAC ACACATTTCA GACGACCCGA TGTCCTGCGA 
GCCATTAAAA ATCTGCCGTA CAAGGGTGGA AATACCATGA CGGGTGATGC AATGGACTAT 
CTGGTGAAAA ACACGTTCAC TCAGGCGGCT GGAGCCAGGA AAGGCTTCCC AAAAGTGGCC 
ATGATCATCA CAGATGGAAA ATCTCAAGAC CCCGTAGAGG AGTACGCCGA ACGGTTGAGG 
AACATCGGAG TGGAGATCTT TGTGCTGGGT ATCAAAGGAG CAGATGAGGA CGAGCTGAAG 
GAGATCGCCT CCAGACCCCA TAGCAAGCAT GTCTATAACG TCCCCAACTT CGACATGATC 
AATCAGGTCC AGAAGGCGCT CATCACTGAA GTTTGCTCTG GCGTGGAGGA GCAGCTAAAT 
TCCCTTGTAA GCGGGGAGGA AGTTGTCGAA CCAGCTTCCA ACCTCAGAGT GACAGATATC 
TCCTCCAAAT CCATGAGAAT TGCGTGGGAT TCATCTCCTG GTGATATCAC AGGCTATAAG 
CTGCAGGTTT TTCCCACTAT TGCGAGCGCC AAAAAGCAGG AGCTCTACAC TGGGCCCACG 
ATGACCTTGG CCAATGTCAG AGACCTGACG CCTGAGATGG AGTATGAGAT CAGTCTGTTT 
GCTCTCAAAG GTCTGACTCC TAGTGTGCCG CAAGTTGTCA CTGAGAAAAC TCAGCCAGTG 
AAAGTATTTA CAGAGTGTTC CCTAGAGGTG GATGTCCAAG CTGACATTGT CCTCCTGGTG 
GATGGGTCAT ATAGTATAGG ACTGCCTAAC TTTGCGAAGG TTAGAGCTTT CCTCGAAGTA 
CTTGTGAATT CTTTTGACAT CGGACCTAAC AAAGTGCAGA TCAGCTTGGT TCAGTACAGC 
AGGGATCCTC ACACTGAGTT CGCCTTGAAT AAATTCGATG ACAATGCAGC CATGGTCAAG 
GCGGTCCGCA CCTTCCCCTA CCGAGGTGGA TCCACCAACA CCGGCAAGGC CATGACATAT 
GTGAGGGAAA AGATCTTTGT TTCGGGCCGT GGCGCTCGTG ATAACGTCCC CCGAGTCATG 
GTGCTTATCA CAGACGGCAA GTCCTCAGAT TCTTTCAAAG ACCCCGCCAA CAAACTGAGG 
GATACAGATG TGGAGATTTT TGCAGTGGGA GTAAAAGATG CCGTACGATC TGAGCTGGAG 
GCTATCGCCA ATGTTCCTGC TGATAACCAT GTGTTTGAGG TGGAGGACTT TGATGCTTTC 
GAGCGGATCT CCAAGGAGCT GACCGCGTCC ATCTGCCTCA GAATTGAGCA GGAGCTCCAT 
AACATCAGGA AAAGAAGTTT GCTTCCCCCA AAGTCACTGA GCTTCTCTGA GGTGACCTCC 
AGGAGTTTCA GGACCTCATG GGTTTCTGAT GGAGAGGATA TCCTGTCTTT TCTGGTGCGC 
TTCAGGCCAG CGGCGGACCT AACTGGAGAC TACATCTCTC TGGTGGTTCC CCATGACATC 
ACCAGTACAA TTCTGCCCAA TCTCACGCCA CTCACCACCT ACGAAGTTAA CGTCATTGCT 
CAGTATGATA AAGGAGACAG CTTTCCTTTG ACGGGCGAGG AGACCACTTT AGAAGAGTTG 
GGTTCTGTTC GTAACCTGAT GGTGAGCGAG GAGGCTGTGG ACCGCTTTCG GGTGTCGTGG 
AGAGCTGCTC CAGGATTAGT GCTGAGGTAC AGACTGCTCT ACGAGCCTGT GAGCGGAGGG 
GATAAACTGG AAGCAGAGAC TGATGGGAAT CAGGTCACCA CTGTGCTCAA TGAACTACTA 
CCGGTCACTA CTTACCGGGT CACCGTTTAC CCTGAGTACG CAAGTGGAAG GGGTCCCTCC 
ATGGACACTG AAGGCACCAC CAAAGAAGTT CGAGGTTCTC CACGCGACTT AAGGGTCTTC 
AATGAGACGG TGTCTAGCAT GAGGGTCTCG TGGCGGGCTG CCCCAGGCAA CGTGCTTCAA 
TATCAGGTGG CCTACAAACC TGACGGTGGG GAGAGGAAAG AAATAAGTGT TAAAGGAGAG 
ACAACAACTG CTGTGCTGAA AAATCTGCTG CCGGACACGG AGTATGAGCT GTTTGTTAGC 
GCACGTTACG TCTCTGGACT TGGCGCACCA CTTATGGGAA CAGGCACCAC GCTGGAAGAA 
CTTGGTTCAC CTTCGAATCT GGTAACTTCA GACGTCACTG AGAACAGTTT TGTAGCATCC 
TGGACCGCCG CCCCTGGAAA CGTCCGTCTT TATCGGGTCA CCTGGAAATC CCTCTTTTCA 
GAGGAAGCTG GCGAGAAGAC TGTCCCCGGG GACGTAACCA CCACTGTTCT GGACGGGCTG 
ACACCAGAGA CCCGCTACAA GGTTTCTGTC TACGCTGTCT ACAGCCGTGC GGAGGGGGCG 
CCACTGCACG GAGAGGAGAC CACAGATGTG TCGGCCGCTA CCAGAGCTCT TACGGTGTCA 
GAAGAAACAG AGAGGACCAT GAAGGTGACA TGGATACCAG CACCTGGCAA TGTTGTCAAT 
TACAGGGTAT CATACAAACC TCAGGGTGAT TTACGCCAAA TGGCCTCCAA AGCACCAGGA 
GGCACCAATT CTATAGTCCT GAGACGCCTG ATCCCCCAGA CCACTTACGA TATCACAGTG 
GTTCCCGTCT ACAAGTTTGG GGATGGAAGA CAAAGGCAAG GAGAAGGAAC TACATTGTCT 
CAATATAAAG CTCCCAGAAA CTTGCAAACT TCGGAGCCCA CTAAAACCAG TTTCAGGGTG 
ACCTGGGATC ATGCTCCTGG AGATGTAAAA GGCTACAAGG TCACTTTCCA TCCCACCGGA 
AAGGAAGTGG ACCTGGGAGA GTTACTCGTA GGCCCCTATG ACAACACTGT GGTTCTGGAG 
GAACTAAGGG CCGGCACTAA GTATACGGTA AATGTGTTTG GTATGTTTGA TGGAGGAGAG 
AGTATGCCAT TGGCTGGAGA GGAGAAGACC ACCCTCTCTG ACGCAGCTTC TGAAAACCTC 
CCTTATACAC CTTCAGATGC CCAGTGTAAA ACCACGGCCC AAGCGGATAT CGTCCTGCTT 
GTTGATGGAT CTTGGAGCAT TGGTCGCCTG AACTTCAAGA CCATACGCAA TTTCATTGCT 
CGCATGGTGG GAGTCTTCGA CATCGGCCCC GATCGTGTCC AGATTGGTCT GGCACAGTAC 
AGCGGAGACC CGAAGACTGA GTGGCATCTC AATGCTCACA GAACCAGGAC TCAGCTGCTG 
GATGCGGTGG CCAATCTACC CTACAAAGGA GGAAACACTC TCACCGGCCT TGCTTTGAAT 
TACATCCTTC AGAATAACTT CAAGCCTAAC GTTGGCATGC GTCCAAACTC CAGAAAGATC 
GGCGTCCTGG TCACTGATGG CAAGTCCCAA GACGACATTG TGGCGAACTC ACAGAACCTG 
CGGGACCAAG GGATTGAACT GTACGCCATT GGTGTAAAGA ACGCAGATGA GAATGAGTTG 
AGAACCATTG CTTCCGATCC CGATGACATT CACATGTACA ACGTGGCTGA TTTCTCCTTC 
CTGCTGGACA TTGTGGATGA CCTCACAAAC AACCTGTGCA ACAGCGTCAA AGGTCCAGGA 
GGAGGACCAG AGCCACCCAC TGATCTGACC ACCTCAGAGG TCACACATTA CTCTTTCCGT 
GCCACTTGGG TGGCTCCTGA CGGGCCTGTG GAGAAGTACA GAGTGGAGTA TGTGAGCACC 
GCTGGAGGAG AACCACAGCA ACAGATGTTT GTGGACGGGA CGGTGACCAC TGTGGTTTTG 
GAAGGTCTCA CTCCGCTGAC CGAGTACATA GTAAAGGTTT ACTCAGTGGT GGGAGAGGAC 
AGCAGCGACC CACTCAGAGG CATAGAGACC ACATTGCCTC TCAGTGCTGT GAGGAGCATG 
AACATCTACA AAGAGCGCTC CACCACCATG AATGTGAGAT GGGACGCAGC CACAGGGGCC 
ACAGGCTACA TGCTTCTCTA CAAGGCCCTT AACGCCACTG AACCCTCTGT GGAGCAGGAG 
ATGCGAGTGA AAGGAATGGT CACTGACGTC CAGCTGAAGA AACTTCTCCC CAACACGGCC 
TACAGTGTTT CGCTCTTCGC CCTGCACGGC GATTACGCCA GTGAACCTCT GATTGATCAA 
GGGGTGACCC TGCCTCTTCC TCCATCCGGT GAGTTGAGGA TTACCGATGT GACCCACAGC 
ACCATGCAGC TGGACTGGGA CGCTGCGCCC GGCGCCGTCC GAAAGTACAT CATCACTTAC 
AAGCCAGAGG AGGGAGAGGC GAAGGAGCTT GAAGTTGACG GAGATGTAAC ATCTAGAGGC 
CTGGATAATC TCATCTCCCA GACTGAATAT GATGTGGCTG TGACTCCGGT TTATGATTCG 
GGCACTGGAA ACCCCATGCT TAACCAGGCC ATTACAGATG TGGTTCCTGC CCCGCAGAAC 
TTGAGGTTCT CGATGGTGAC CCAGACCAGT TTTAGGGTTC ACTGGGAGCA CGGCGCTCCT 
GATGTCTCCC TCTACCGTAT TGGCTGGAAA AAGAAAGGAA CGACCGAGTT TCAAAATGAT 
ATCCTGAGTA GTGAAGACAC TACATATGAT CTGGTGGACC TGGAGCCAGA CACACCTTAT 
GATGTTTCGG TCACTGCCAT TTACCCTGAT GAGTCTGAGA GCGAGGATCT GCTGGGCACT 
GAACGCACTT TGGCCAAGCT TGCTACACCA CCTCCCAGTG GTCCTCCTCA AAACCTCCAG 
GTGTTCGACG AGACCACCAC CTCCCTCACG ACTAAGTGGG ACCACGCGCC TGGACGAGTT 
CAGAACTACA AAATCACCTA CGTACCGCTT ACTGGAGGAC GATCACAGTC GACGCAGGTC 
GGAGGAAAGA AAAACACAGT GATCCTGCAG AAACTGACTC CAGACACTCC TTACTCCATC 
ACTGTTGCTG CGGTCTACGC CAGCGGAGAG GCCAGAGACA TTTCTGGCCA AGGAAAGACA 
TTGCCGCTGG GCAGTGTCAG GAATCTTCAG GTCACTGACC CCACAGTTAG CACTCTGAAT 
GTGCGCTGGG AGCCGGCGGA GGGAAGCGTT CGCCAGTACA GGATCTACTA CCAGCCCGTC 
GCAGGGGGCG CAGAGGACAT GGTCCAGGTT CCTGGAAGCA CCACCAGCAC CGTTCTGAAG 
AACCTGCAAT CTGACACTCA GTACAGAGTG ACGGTGGTGC CAGTCTACGC TCCTGGAGAG 
GGCAAGCGCA TGTCGGAGAC CGGAAAAACA CTGGTTCGGA CCCCTGCCAA GAACATCCAG 
GTTTACAACC CCACCACAAA CACCCTGAAC GTGCGCTGGG AGCCGGCCTC AGGACAGGTG 
CAGCAGTACC GCGTGGCTTA CGCCCCTCTC TCCGGGATCC GACCCTCTCA GTCGGTGCTG 
GTTCCAGGAA ACACCCACAC GGCTCTGCTG CAGCAGCTGG AATCAGACAC TGACTACTCT 
GTGAGTGTGG TGGCTCTGTA TGCTGATGGA GAGGGCTCGT CTGTGTCTGA TAATGGCAAA 
ACACTGCCCC GCAGTGGCCC CAGGAACCTG CGGGTTTATG ATCCCACCAC CAGCACGCTG 
TCGGTGGGCT GGGAACATGC AGAAGGGCCT GTGATTCAGT ACCGCATTGG CTACGCACCC 
ACCACTGGAG ATCCCATTGA AGAATTTACA ACTGTCCCAG GCAACCGTAA CTCGGTCATC 
CTGCAAAACC TGCAGCCAGA CACGCCGTAC AACATCAACG TAGAAGCCAT TTATCAAGAT 
GGCCCTGGAG GACGACTCAG TGGAGATGGA AAAACATTGG ATCTCCTTGG GCCTCGTAAC 
CTGCGTGTTT CAGACGAGTG GTACACACGT TTCCGAGTGT CCTGGGATCC TGCAGCCGCA 
CGGGTGAATG GATATAAACT GGTCTATACA CCAGTGGGCA CAACCGACAC CATGGAAGCC 
TTCGTTGGAG ACGTGACTTC ACACCACCTG AACAACCTCC AGCCTGGCAC CACATACGAC 
CTGAAGGTTT ACGCTCAGTA TGATGCGGGC TTGAGCGGCG CTCTGGTCGG ACAGGGAACC 
ACCCTGTATC TCAACGTGAC CGACCTCACT TCCTACAACG TGGGCTACGA CTCTTTCTGC 
ATCAGATGGA CTCCTCATCG AGCTGCCACC TCCTACAGAC TCAAAGTCAA CCCTTTCAAC 
CCATCTCACC GTGGAGCTCA GGAGGTCACG ATCACTGCGG CTGAGAGCCA GTACTGTTTT 
GACGGCCTGA CGCCAGACGC GCTATACAAC GCCACCGTCT ACACGCAGAC CCCCAACCGA 
GAGGGGCCCG GAGTCAGTGT CAAAGAGCGC ACACTGGTGA AGCCCACCGT CGCCCCAACA 
CTACCTCCAA CTCCTCCACC TCCAGCCACC ATTCCTTCTG CACTCGAGGT GTGCAAAGGT 
GCCAAGGCTG ACCTGGTGTT CCTGATCGAT GGCTCGTGGA GTATCGGAGA TGACAGCTTC 
GCTAAAGTCC GGCAGTTTGT GTTCAGCATG ATTGGGGCGT TTGATGTGGT CAGCCATGAG 
GGCATGCAGG TGTCTTTCGT GCAGTACAGC GACGACGCCA AAACTGAGTT CAAGCTCAAC 
ACCTACCACG ACAAGGGAAT GGTGCTGTCT GCTCTTCAGA TGGTCAATTA TAGAGGAGGA 
AACACCAAAA CAGGTGCTGC ACTGAAGCAT GTTGGAGAAA AAGTGTTCAC CTCGGACAAT 
GGCATGAGGA GAACCGTTCC CAAGGTACTG GTTGTGGTGA CCGATGGACG TTCTCAAGAT 
GACGTGAAGA AGAGCGCTGC TAAACTACAG CATGCAGGTT ACAGTGTGTT TGTGGTGGGT 
GTCGCTGACA TTGACGTTAA TGAACTGAGG AACATTGGAA GCAAGCCCAG TGACAGACAC 
GTCTTTATCG TCGACAACTT CGATGCTTTC GCCAAGATCC AAGACAACCT GATCACGTTC 
ATCTGTGAAA CGGCAACGTC CACATGCCCT TTGATTTACC TCAATGGATT CACATCACCA 
GGCTTCAGGA TGCTGGAAGC TTTCAACATC ACTGACAAGA CCTACGCAGG CATGAACGGC 
GTCTCTCTGG AAACCGGCTC CTTCAACAGC TTCATCGCAT ACAGACTCCA TAAGGACGCC 
TTCATTTCCC AGCCAACAAA GGAAATCCAT CCAGATGGTC TTCCTCCATC CTACACCCTC 
ATCCTCCTCT TCCGTCTGCT CCCGGACACC GGCAAGGATG CGTTTGATTT ATGGCAGATC 
TCAGATAAGA ACAATAACCC TGAGGTCGGG CTCACCATTG ACCCCTCCAG CAGAGTGATC 
TCCTTCTACA ATAAAGACAC TAGAGGGGAA ATTCAGAAAG TCTCCTTTGA TAACGACCAC 
GTGAAGAAGA TTTTCCACGG CAGTTTCCAC AAGCTTCACA TTTCTATTTC GCCGAAAAAT 
GTCAAACTCA ACATCGACTG CCAAGAGGTG GCTGAGAAAC AGATCAAAGC AGCCAACAAC 
GTCTCCACAG ATGGCTACGA AGTCCTTGGA AAAATGACAA AGTCTGGCGG TTCAAAGAAG 
CAGTCGGCCA CATTCCAGCT CCAGATGTTT GATATTGTCT GCAGTTTGGG CTGGACAAGT 
CGAGACAAAT GTTGTGATAT TCCAGCAATG CGAGATGAAG CCAAGTGTCC TGCTCTTCCT 
CACTCCTGCA CATGTGCTCA GGACAGTGTT GGTCCTCCAG GCCCAGCGGG TCCTCAAGGC 
AGTCCTGGTA CAAAAGGCCC ACGAGGCGAG AGAGGAGAGT CTGGTCCTAC GGGTCCAATG 
GGTCCACGTG GAGAGCAGGG TCTGCCAGGG CAGATGGGGC CACCGGGTCC ACAGGGCCCC 
AATGGACTAT CCCTTCCCGG AGAACCAGGG CGACCGGGAC CTAAGGGAGA CCCCGGTGAC 
TCAGGTTTAC CTGGACAGAG AGGCCCGGTT GGCGCTGCAG GTCCACTCGG CCCTGTTGGA 
CCCGCTGGAG CAAGAGGTCC ACCAGGAAGT GAAGGCCCAT CTGGACCAAG AGGTCCATCG 
GGACCAATGG GACCACCGGG AGCTCCCGGA ATGCCAGGAA TCACCGGAAA ACCAGGAAAA 
CCCGGAGACA CTGGCTTTCC TGGACCTGTT GGCATGAAAG GAGAGAAGGG TGAAAGAGGA 
GACACTGCGT CACAGAATAT GATGCGCTCT GTAGCGAGAC AAGTCTGCGA ACAGCTAGTC 
AGCAGACAAA TGAGCAGAAT TGATATGATG ATGAACCAGA TCCCCAGCAA CTACAGGAGT 
AACAGCCCCG GCCCCGCTGG ACCTCCAGGC CCACCTGGAA ACCAGGGACC CCATGGAGAG 
CCTGGTCAGC CCGGACCCAA TGGCTTCCCA GGAAATCCAG GTTTACCAGG CTCTCCTGGA 
GAAAGAGGAG TGCCTGGAGA GAAGGGTGAA CGAGGCAGCC CCGGCATTGG CACTCGAGGA 
CAGAGAGGAT TACCTGGACC TCCTGGGCCA CCAGGGCAGT CTCAGACCGG TCCCCCTGGT 
CCCTCAGGCT CACCCGGGCC CCGAGGACCA CCAGGACAAC ATGGTTCTTC TGGAGTCACA 
GGACCACCAG GACCTCCAGG ATACTGTGAC TCCTCTCAGT GCGGCATGCC TTACAACGGA 
CAAGGCTACC CAGGTTTTGC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002665305.3
CDS	180..9383
Translation	MKIRLSLAAAALLTLVLSSVEAQVEPPSDLKFKILNENTVQMSWRKPSSQIQGYRIQVTSDTDDSKELSLPASSTSTSITDLTPDVDYSVSINSYDGAEESIPILGQITIQSSNTSGPRRPQSEAVKCSVSAVADLVFLVDGSWSVGRENFRFIRSFIGAMAGAFDIEEDKTRVAVVQYSSDTRTEFSLNTHFRRPDVLRAIKNLPYKGGNTMTGDAMDYLVKNTFTQAAGARKGFPKVAMIITDGKSQDPVEEYAERLRNIGVEIFVLGIKGADEDELKEIASRPHSKHVYNVPNFDMINQVQKALITEVCSGVEEQLNSLVSGEEVVEPASNLRVTDISSKSMRIAWDSSPGDITGYKLQVFPTIASAKKQELYTGPTMTLANVRDLTPEMEYEISLFALKGLTPSVPQVVTEKTQPVKVFTECSLEVDVQADIVLLVDGSYSIGLPNFAKVRAFLEVLVNSFDIGPNKVQISLVQYSRDPHTEFALNKFDDNAAMVKAVRTFPYRGGSTNTGKAMTYVREKIFVSGRGARDNVPRVMVLITDGKSSDSFKDPANKLRDTDVEIFAVGVKDAVRSELEAIANVPADNHVFEVEDFDAFERISKELTASICLRIEQELHNIRKRSLLPPKSLSFSEVTSRSFRTSWVSDGEDILSFLVRFRPAADLTGDYISLVVPHDITSTILPNLTPLTTYEVNVIAQYDKGDSFPLTGEETTLEELGSVRNLMVSEEAVDRFRVSWRAAPGLVLRYRLLYEPVSGGDKLEAETDGNQVTTVLNELLPVTTYRVTVYPEYASGRGPSMDTEGTTKEVRGSPRDLRVFNETVSSMRVSWRAAPGNVLQYQVAYKPDGGERKEISVKGETTTAVLKNLLPDTEYELFVSARYVSGLGAPLMGTGTTLEELGSPSNLVTSDVTENSFVASWTAAPGNVRLYRVTWKSLFSEEAGEKTVPGDVTTTVLDGLTPETRYKVSVYAVYSRAEGAPLHGEETTDVSAATRALTVSEETERTMKVTWIPAPGNVVNYRVSYKPQGDLRQMASKAPGGTNSIVLRRLIPQTTYDITVVPVYKFGDGRQRQGEGTTLSQYKAPRNLQTSEPTKTSFRVTWDHAPGDVKGYKVTFHPTGKEVDLGELLVGPYDNTVVLEELRAGTKYTVNVFGMFDGGESMPLAGEEKTTLSDAASENLPYTPSDAQCKTTAQADIVLLVDGSWSIGRLNFKTIRNFIARMVGVFDIGPDRVQIGLAQYSGDPKTEWHLNAHRTRTQLLDAVANLPYKGGNTLTGLALNYILQNNFKPNVGMRPNSRKIGVLVTDGKSQDDIVANSQNLRDQGIELYAIGVKNADENELRTIASDPDDIHMYNVADFSFLLDIVDDLTNNLCNSVKGPGGGPEPPTDLTTSEVTHYSFRATWVAPDGPVEKYRVEYVSTAGGEPQQQMFVDGTVTTVVLEGLTPLTEYIVKVYSVVGEDSSDPLRGIETTLPLSAVRSMNIYKERSTTMNVRWDAATGATGYMLLYKALNATEPSVEQEMRVKGMVTDVQLKKLLPNTAYSVSLFALHGDYASEPLIDQGVTLPLPPSGELRITDVTHSTMQLDWDAAPGAVRKYIITYKPEEGEAKELEVDGDVTSRGLDNLISQTEYDVAVTPVYDSGTGNPMLNQAITDVVPAPQNLRFSMVTQTSFRVHWEHGAPDVSLYRIGWKKKGTTEFQNDILSSEDTTYDLVDLEPDTPYDVSVTAIYPDESESEDLLGTERTLAKLATPPPSGPPQNLQVFDETTTSLTTKWDHAPGRVQNYKITYVPLTGGRSQSTQVGGKKNTVILQKLTPDTPYSITVAAVYASGEARDISGQGKTLPLGSVRNLQVTDPTVSTLNVRWEPAEGSVRQYRIYYQPVAGGAEDMVQVPGSTTSTVLKNLQSDTQYRVTVVPVYAPGEGKRMSETGKTLVRTPAKNIQVYNPTTNTLNVRWEPASGQVQQYRVAYAPLSGIRPSQSVLVPGNTHTALLQQLESDTDYSVSVVALYADGEGSSVSDNGKTLPRSGPRNLRVYDPTTSTLSVGWEHAEGPVIQYRIGYAPTTGDPIEEFTTVPGNRNSVILQNLQPDTPYNINVEAIYQDGPGGRLSGDGKTLDLLGPRNLRVSDEWYTRFRVSWDPAAARVNGYKLVYTPVGTTDTMEAFVGDVTSHHLNNLQPGTTYDLKVYAQYDAGLSGALVGQGTTLYLNVTDLTSYNVGYDSFCIRWTPHRAATSYRLKVNPFNPSHRGAQEVTITAAESQYCFDGLTPDALYNATVYTQTPNREGPGVSVKERTLVKPTVAPTLPPTPPPPATIPSALEVCKGAKADLVFLIDGSWSIGDDSFAKVRQFVFSMIGAFDVVSHEGMQVSFVQYSDDAKTEFKLNTYHDKGMVLSALQMVNYRGGNTKTGAALKHVGEKVFTSDNGMRRTVPKVLVVVTDGRSQDDVKKSAAKLQHAGYSVFVVGVADIDVNELRNIGSKPSDRHVFIVDNFDAFAKIQDNLITFICETATSTCPLIYLNGFTSPGFRMLEAFNITDKTYAGMNGVSLETGSFNSFIAYRLHKDAFISQPTKEIHPDGLPPSYTLILLFRLLPDTGKDAFDLWQISDKNNNPEVGLTIDPSSRVISFYNKDTRGEIQKVSFDNDHVKKIFHGSFHKLHISISPKNVKLNIDCQEVAEKQIKAANNVSTDGYEVLGKMTKSGGSKKQSATFQLQMFDIVCSLGWTSRDKCCDIPAMRDEAKCPALPHSCTCAQDSVGPPGPAGPQGSPGTKGPRGERGESGPTGPMGPRGEQGLPGQMGPPGPQGPNGLSLPGEPGRPGPKGDPGDSGLPGQRGPVGAAGPLGPVGPAGARGPPGSEGPSGPRGPSGPMGPPGAPGMPGITGKPGKPGDTGFPGPVGMKGEKGERGDTASQNMMRSVARQVCEQLVSRQMSRIDMMMNQIPSNYRSNSPGPAGPPGPPGNQGPHGEPGQPGPNGFPGNPGLPGSPGERGVPGEKGERGSPGIGTRGQRGLPGPPGPPGQSQTGPPGPSGSPGPRGPPGQHGSSGVTGPPGPPGYCDSSQCGMPYNGQGYPGFA

Target ORF information:

RefSeq Version	XM_002665259.6
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type XII, alpha 1a (col12a1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002665259.6

ORF Insert Sequence:

ATGAAGATCA GGTTGTCTTT GGCCGCTGCT GCGCTCTTGA CTCTAGTACT CAGTTCAGTA 
GAGGCTCAAG TGGAGCCTCC GTCAGACTTA AAATTTAAAA TCCTGAATGA AAACACAGTG 
CAAATGTCAT GGAGGAAGCC CTCGTCTCAG ATACAGGGCT ACAGAATACA GGTCACGTCA 
GATACAGATG ATTCTAAAGA ATTGAGTCTT CCTGCCTCGT CCACAAGCAC ATCAATTACA 
GATCTAACTC CAGATGTAGA CTATTCAGTG TCAATCAATT CATATGATGG TGCTGAAGAA 
AGTATCCCTA TCCTTGGACA AATCACAATT CAATCCAGCA ACACAAGTGG ACCAAGGAGG 
CCACAGTCAG AGGCTGTCAA GTGTTCAGTG AGTGCCGTTG CGGATCTGGT TTTCCTGGTG 
GACGGCTCTT GGAGTGTGGG AAGAGAAAAC TTTAGATTTA TCCGGAGTTT CATTGGGGCC 
ATGGCCGGGG CCTTCGACAT TGAAGAGGAT AAGACCAGAG TGGCTGTGGT TCAGTACAGC 
TCGGACACCA GGACAGAGTT CAGTCTAAAC ACACATTTCA GACGACCCGA TGTCCTGCGA 
GCCATTAAAA ATCTGCCGTA CAAGGGTGGA AATACCATGA CGGGTGATGC AATGGACTAT 
CTGGTGAAAA ACACGTTCAC TCAGGCGGCT GGAGCCAGGA AAGGCTTCCC AAAAGTGGCC 
ATGATCATCA CAGATGGAAA ATCTCAAGAC CCCGTAGAGG AGTACGCCGA ACGGTTGAGG 
AACATCGGAG TGGAGATCTT TGTGCTGGGT ATCAAAGGAG CAGATGAGGA CGAGCTGAAG 
GAGATCGCCT CCAGACCCCA TAGCAAGCAT GTCTATAACG TCCCCAACTT CGACATGATC 
AATCAGGTCC AGAAGGCGCT CATCACTGAA GTTTGCTCTG GCGTGGAGGA GCAGCTAAAT 
TCCCTTGTAA GCGGGGAGGA AGTTGTCGAA CCAGCTTCCA ACCTCAGAGT GACAGATATC 
TCCTCCAAAT CCATGAGAAT TGCGTGGGAT TCATCTCCTG GTGATATCAC AGGCTATAAG 
CTGCAGGTTT TTCCCACTAT TGCGAGCGCC AAAAAGCAGG AGCTCTACAC TGGGCCCACG 
ATGACCTTGG CCAATGTCAG AGACCTGACG CCTGAGATGG AGTATGAGAT CAGTCTGTTT 
GCTCTCAAAG GTCTGACTCC TAGTGTGCCG CAAGTTGTCA CTGAGAAAAC TCAGCCAGTG 
AAAGTATTTA CAGAGTGTTC CCTAGAGGTG GATGTCCAAG CTGACATTGT CCTCCTGGTG 
GATGGGTCAT ATAGTATAGG ACTGCCTAAC TTTGCGAAGG TTAGAGCTTT CCTCGAAGTA 
CTTGTGAATT CTTTTGACAT CGGACCTAAC AAAGTGCAGA TCAGCTTGGT TCAGTACAGC 
AGGGATCCTC ACACTGAGTT CGCCTTGAAT AAATTCGATG ACAATGCAGC CATGGTCAAG 
GCGGTCCGCA CCTTCCCCTA CCGAGGTGGA TCCACCAACA CCGGCAAGGC CATGACATAT 
GTGAGGGAAA AGATCTTTGT TTCGGGCCGT GGCGCTCGTG ATAACGTCCC CCGAGTCATG 
GTGCTTATCA CAGACGGCAA GTCCTCAGAT TCTTTCAAAG ACCCCGCCAA CAAACTGAGG 
GATACAGATG TGGAGATTTT TGCAGTGGGA GTAAAAGATG CCGTACGATC TGAGCTGGAG 
GCTATCGCCA ATGTTCCTGC TGATAACCAT GTGTTTGAGG TGGAGGACTT TGATGCTTTC 
GAGCGGATCT CCAAGGAGCT GACCGCGTCC ATCTGCCTCA GAATTGAGCA GGAGCTCCAT 
AACATCAGGA AAAGAAGTTT GCTTCCCCCA AAGTCACTGA GCTTCTCTGA GGTGACCTCC 
AGGAGTTTCA GGACCTCATG GGTTTCTGAT GGAGAGGATA TCCTGTCTTT TCTGGTGCGC 
TTCAGGCCAG CGGCGGACCT AACTGGAGAC TACATCTCTC TGGTGGTTCC CCATGACATC 
ACCAGTACAA TTCTGCCCAA TCTCACGCCA CTCACCACCT ACGAAGTTAA CGTCATTGCT 
CAGTATGATA AAGGAGACAG CTTTCCTTTG ACGGGCGAGG AGACCACTTT AGAAGAGTTG 
GGTTCTGTTC GTAACCTGAT GGTGAGCGAG GAGGCTGTGG ACCGCTTTCG GGTGTCGTGG 
AGAGCTGCTC CAGGATTAGT GCTGAGGTAC AGACTGCTCT ACGAGCCTGT GAGCGGAGGG 
GATAAACTGG AAGCAGAGAC TGATGGGAAT CAGGTCACCA CTGTGCTCAA TGAACTACTA 
CCGGTCACTA CTTACCGGGT CACCGTTTAC CCTGAGTACG CAAGTGGAAG GGGTCCCTCC 
ATGGACACTG AAGGCACCAC CAAAGAAGTT CGAGGTTCTC CACGCGACTT AAGGGTCTTC 
AATGAGACGG TGTCTAGCAT GAGGGTCTCG TGGCGGGCTG CCCCAGGCAA CGTGCTTCAA 
TATCAGGTGG CCTACAAACC TGACGGTGGG GAGAGGAAAG AAATAAGTGT TAAAGGAGAG 
ACAACAACTG CTGTGCTGAA AAATCTGCTG CCGGACACGG AGTATGAGCT GTTTGTTAGC 
GCACGTTACG TCTCTGGACT TGGCGCACCA CTTATGGGAA CAGGCACCAC GCTGGAAGAA 
CTTGGTTCAC CTTCGAATCT GGTAACTTCA GACGTCACTG AGAACAGTTT TGTAGCATCC 
TGGACCGCCG CCCCTGGAAA CGTCCGTCTT TATCGGGTCA CCTGGAAATC CCTCTTTTCA 
GAGGAAGCTG GCGAGAAGAC TGTCCCCGGG GACGTAACCA CCACTGTTCT GGACGGGCTG 
ACACCAGAGA CCCGCTACAA GGTTTCTGTC TACGCTGTCT ACAGCCGTGC GGAGGGGGCG 
CCACTGCACG GAGAGGAGAC CACAGATGTG TCGGCCGCTA CCAGAGCTCT TACGGTGTCA 
GAAGAAACAG AGAGGACCAT GAAGGTGACA TGGATACCAG CACCTGGCAA TGTTGTCAAT 
TACAGGGTAT CATACAAACC TCAGGGTGAT TTACGCCAAA TGGCCTCCAA AGCACCAGGA 
GGCACCAATT CTATAGTCCT GAGACGCCTG ATCCCCCAGA CCACTTACGA TATCACAGTG 
GTTCCCGTCT ACAAGTTTGG GGATGGAAGA CAAAGGCAAG GAGAAGGAAC TACATTGTCT 
CAATATAAAG CTCCCAGAAA CTTGCAAACT TCGGAGCCCA CTAAAACCAG TTTCAGGGTG 
ACCTGGGATC ATGCTCCTGG AGATGTAAAA GGCTACAAGG TCACTTTCCA TCCCACCGGA 
AAGGAAGTGG ACCTGGGAGA GTTACTCGTA GGCCCCTATG ACAACACTGT GGTTCTGGAG 
GAACTAAGGG CCGGCACTAA GTATACGGTA AATGTGTTTG GTATGTTTGA TGGAGGAGAG 
AGTATGCCAT TGGCTGGAGA GGAGAAGACC ACCCTCTCTG ACGCAGCTTC TGAAAACCTC 
CCTTATACAC CTTCAGATGC CCAGTGTAAA ACCACGGCCC AAGCGGATAT CGTCCTGCTT 
GTTGATGGAT CTTGGAGCAT TGGTCGCCTG AACTTCAAGA CCATACGCAA TTTCATTGCT 
CGCATGGTGG GAGTCTTCGA CATCGGCCCC GATCGTGTCC AGATTGGTCT GGCACAGTAC 
AGCGGAGACC CGAAGACTGA GTGGCATCTC AATGCTCACA GAACCAGGAC TCAGCTGCTG 
GATGCGGTGG CCAATCTACC CTACAAAGGA GGAAACACTC TCACCGGCCT TGCTTTGAAT 
TACATCCTTC AGAATAACTT CAAGCCTAAC GTTGGCATGC GTCCAAACTC CAGAAAGATC 
GGCGTCCTGG TCACTGATGG CAAGTCCCAA GACGACATTG TGGCGAACTC ACAGAACCTG 
CGGGACCAAG GGATTGAACT GTACGCCATT GGTGTAAAGA ACGCAGATGA GAATGAGTTG 
AGAACCATTG CTTCCGATCC CGATGACATT CACATGTACA ACGTGGCTGA TTTCTCCTTC 
CTGCTGGACA TTGTGGATGA CCTCACAAAC AACCTGTGCA ACAGCGTCAA AGGTCCAGGA 
GGAGGACCAG AGCCACCCAC TGATCTGACC ACCTCAGAGG TCACACATTA CTCTTTCCGT 
GCCACTTGGG TGGCTCCTGA CGGGCCTGTG GAGAAGTACA GAGTGGAGTA TGTGAGCACC 
GCTGGAGGAG AACCACAGCA ACAGATGTTT GTGGACGGGA CGGTGACCAC TGTGGTTTTG 
GAAGGTCTCA CTCCGCTGAC CGAGTACATA GTAAAGGTTT ACTCAGTGGT GGGAGAGGAC 
AGCAGCGACC CACTCAGAGG CATAGAGACC ACATTGCCTC TCAGTGCTGT GAGGAGCATG 
AACATCTACA AAGAGCGCTC CACCACCATG AATGTGAGAT GGGACGCAGC CACAGGGGCC 
ACAGGCTACA TGCTTCTCTA CAAGGCCCTT AACGCCACTG AACCCTCTGT GGAGCAGGAG 
ATGCGAGTGA AAGGAATGGT CACTGACGTC CAGCTGAAGA AACTTCTCCC CAACACGGCC 
TACAGTGTTT CGCTCTTCGC CCTGCACGGC GATTACGCCA GTGAACCTCT GATTGATCAA 
GGGGTGACCC TGCCTCTTCC TCCATCCGGT GAGTTGAGGA TTACCGATGT GACCCACAGC 
ACCATGCAGC TGGACTGGGA CGCTGCGCCC GGCGCCGTCC GAAAGTACAT CATCACTTAC 
AAGCCAGAGG AGGGAGAGGC GAAGGAGCTT GAAGTTGACG GAGATGTAAC ATCTAGAGGC 
CTGGATAATC TCATCTCCCA GACTGAATAT GATGTGGCTG TGACTCCGGT TTATGATTCG 
GGCACTGGAA ACCCCATGCT TAACCAGGCC ATTACAGATG TGGTTCCTGC CCCGCAGAAC 
TTGAGGTTCT CGATGGTGAC CCAGACCAGT TTTAGGGTTC ACTGGGAGCA CGGCGCTCCT 
GATGTCTCCC TCTACCGTAT TGGCTGGAAA AAGAAAGGAA CGACCGAGTT TCAAAATGAT 
ATCCTGAGTA GTGAAGACAC TACATATGAT CTGGTGGACC TGGAGCCAGA CACACCTTAT 
GATGTTTCGG TCACTGCCAT TTACCCTGAT GAGTCTGAGA GCGAGGATCT GCTGGGCACT 
GAACGCACTT TGGCCAAGCT TGCTACACCA CCTCCCAGTG GTCCTCCTCA AAACCTCCAG 
GTGTTCGACG AGACCACCAC CTCCCTCACG ACTAAGTGGG ACCACGCGCC TGGACGAGTT 
CAGAACTACA AAATCACCTA CGTACCGCTT ACTGGAGGAC GATCACAGTC GACGCAGGTC 
GGAGGAAAGA AAAACACAGT GATCCTGCAG AAACTGACTC CAGACACTCC TTACTCCATC 
ACTGTTGCTG CGGTCTACGC CAGCGGAGAG GCCAGAGACA TTTCTGGCCA AGGAAAGACA 
TTGCCGCTGG GCAGTGTCAG GAATCTTCAG GTCACTGACC CCACAGTTAG CACTCTGAAT 
GTGCGCTGGG AGCCGGCGGA GGGAAGCGTT CGCCAGTACA GGATCTACTA CCAGCCCGTC 
GCAGGGGGCG CAGAGGACAT GGTCCAGGTT CCTGGAAGCA CCACCAGCAC CGTTCTGAAG 
AACCTGCAAT CTGACACTCA GTACAGAGTG ACGGTGGTGC CAGTCTACGC TCCTGGAGAG 
GGCAAGCGCA TGTCGGAGAC CGGAAAAACA CTGGTTCGGA CCCCTGCCAA GAACATCCAG 
GTTTACAACC CCACCACAAA CACCCTGAAC GTGCGCTGGG AGCCGGCCTC AGGACAGGTG 
CAGCAGTACC GCGTGGCTTA CGCCCCTCTC TCCGGGATCC GACCCTCTCA GTCGGTGCTG 
GTTCCAGGAA ACACCCACAC GGCTCTGCTG CAGCAGCTGG AATCAGACAC TGACTACTCT 
GTGAGTGTGG TGGCTCTGTA TGCTGATGGA GAGGGCTCGT CTGTGTCTGA TAATGGCAAA 
ACACTGCCCC GCAGTGGCCC CAGGAACCTG CGGGTTTATG ATCCCACCAC CAGCACGCTG 
TCGGTGGGCT GGGAACATGC AGAAGGGCCT GTGATTCAGT ACCGCATTGG CTACGCACCC 
ACCACTGGAG ATCCCATTGA AGAATTTACA ACTGTCCCAG GCAACCGTAA CTCGGTCATC 
CTGCAAAACC TGCAGCCAGA CACGCCGTAC AACATCAACG TAGAAGCCAT TTATCAAGAT 
GGCCCTGGAG GACGACTCAG TGGAGATGGA AAAACATTGG ATCTCCTTGG GCCTCGTAAC 
CTGCGTGTTT CAGACGAGTG GTACACACGT TTCCGAGTGT CCTGGGATCC TGCAGCCGCA 
CGGGTGAATG GATATAAACT GGTCTATACA CCAGTGGGCA CAACCGACAC CATGGAAGCC 
TTCGTTGGAG ACGTGACTTC ACACCACCTG AACAACCTCC AGCCTGGCAC CACATACGAC 
CTGAAGGTTT ACGCTCAGTA TGATGCGGGC TTGAGCGGCG CTCTGGTCGG ACAGGGAACC 
ACCCTGTATC TCAACGTGAC CGACCTCACT TCCTACAACG TGGGCTACGA CTCTTTCTGC 
ATCAGATGGA CTCCTCATCG AGCTGCCACC TCCTACAGAC TCAAAGTCAA CCCTTTCAAC 
CCATCTCACC GTGGAGCTCA GGAGGTCACG ATCACTGCGG CTGAGAGCCA GTACTGTTTT 
GACGGCCTGA CGCCAGACGC GCTATACAAC GCCACCGTCT ACACGCAGAC CCCCAACCGA 
GAGGGGCCCG GAGTCAGTGT CAAAGAGCGC ACACTGGTGA AGCCCACCGT CGCCCCAACA 
CTACCTCCAA CTCCTCCACC TCCAGCCACC ATTCCTTCTG CACTCGAGGT GTGCAAAGGT 
GCCAAGGCTG ACCTGGTGTT CCTGATCGAT GGCTCGTGGA GTATCGGAGA TGACAGCTTC 
GCTAAAGTCC GGCAGTTTGT GTTCAGCATG ATTGGGGCGT TTGATGTGGT CAGCCATGAG 
GGCATGCAGG TGTCTTTCGT GCAGTACAGC GACGACGCCA AAACTGAGTT CAAGCTCAAC 
ACCTACCACG ACAAGGGAAT GGTGCTGTCT GCTCTTCAGA TGGTCAATTA TAGAGGAGGA 
AACACCAAAA CAGGTGCTGC ACTGAAGCAT GTTGGAGAAA AAGTGTTCAC CTCGGACAAT 
GGCATGAGGA GAACCGTTCC CAAGGTACTG GTTGTGGTGA CCGATGGACG TTCTCAAGAT 
GACGTGAAGA AGAGCGCTGC TAAACTACAG CATGCAGGTT ACAGTGTGTT TGTGGTGGGT 
GTCGCTGACA TTGACGTTAA TGAACTGAGG AACATTGGAA GCAAGCCCAG TGACAGACAC 
GTCTTTATCG TCGACAACTT CGATGCTTTC GCCAAGATCC AAGACAACCT GATCACGTTC 
ATCTGTGAAA CGGCAACGTC CACATGCCCT TTGATTTACC TCAATGGATT CACATCACCA 
GGCTTCAGGA TGCTGGAAGC TTTCAACATC ACTGACAAGA CCTACGCAGG CATGAACGGC 
GTCTCTCTGG AAACCGGCTC CTTCAACAGC TTCATCGCAT ACAGACTCCA TAAGGACGCC 
TTCATTTCCC AGCCAACAAA GGAAATCCAT CCAGATGGTC TTCCTCCATC CTACACCCTC 
ATCCTCCTCT TCCGTCTGCT CCCGGACACC GGCAAGGATG CGTTTGATTT ATGGCAGATC 
TCAGATAAGA ACAATAACCC TGAGGTCGGG CTCACCATTG ACCCCTCCAG CAGAGTGATC 
TCCTTCTACA ATAAAGACAC TAGAGGGGAA ATTCAGAAAG TCTCCTTTGA TAACGACCAC 
GTGAAGAAGA TTTTCCACGG CAGTTTCCAC AAGCTTCACA TTTCTATTTC GCCGAAAAAT 
GTCAAACTCA ACATCGACTG CCAAGAGGTG GCTGAGAAAC AGATCAAAGC AGCCAACAAC 
GTCTCCACAG ATGGCTACGA AGTCCTTGGA AAAATGACAA AGTCTGGCGG TTCAAAGAAG 
CAGTCGGCCA CATTCCAGCT CCAGATGTTT GATATTGTCT GCAGTTTGGG CTGGACAAGT 
CGAGACAAAT GTTGTGATAT TCCAGCAATG CGAGATGAAG CCAAGTGTCC TGCTCTTCCT 
CACTCCTGCA CATGTGCTCA GGACAGTGTT GGTCCTCCAG GCCCAGCGGG TCCTCAAGGC 
AGTCCTGGTA CAAAAGGCCC ACGAGGCGAG AGAGGAGAGT CTGGTCCTAC GGGTCCAATG 
GGTCCACGTG GAGAGCAGGG TCTGCCAGGG CAGATGGGGC CACCGGGTCC ACAGGGCCCC 
AATGGACTAT CCCTTCCCGG AGAACCAGGG CGACCGGGAC CTAAGGGAGA CCCCGGTGAC 
TCAGGTTTAC CTGGACAGAG AGGCCCGGTT GGCGCTGCAG GTCCACTCGG CCCTGTTGGA 
CCCGCTGGAG CAAGAGGTCC ACCAGGAAGT GAAGGCCCAT CTGGACCAAG AGGTCCATCG 
GGACCAATGG GACCACCGGG AGCTCCCGGA ATGCCAGGAA TCACCGGAAA ACCAGGAAAA 
CCCGGAGACA CTGGCTTTCC TGGACCTGTT GGCATGAAAG GAGAGAAGGG TGAAAGAGGA 
GACACTGCGT CACAGAATAT GATGCGCTCT GTAGCGAGAC AAGTCTGCGA ACAGCTAGTC 
AGCAGACAAA TGAGCAGAAT TGATATGATG ATGAACCAGA TCCCCAGCAA CTACAGGAGT 
AACAGCCCCG GCCCCGCTGG ACCTCCAGGC CCACCTGGAA ACCAGGGACC CCATGGAGAG 
CCTGGTCAGC CCGGACCCAA TGGCTTCCCA GGAAATCCAG GTTTACCAGG CTCTCCTGGA 
GAAAGAGGAG TGCCTGGAGA GAAGGGTGAA CGAGGCAGCC CCGGCATTGG CACTCGAGGA 
CAGAGAGGAT TACCTGGACC TCCTGGGCCA CCAGGGCAGT CTCAGACCGG TCCCCCTGGT 
CCCTCAGGCT CACCCGGGCC CCGAGGACCA CCAGGACAAC ATGGTTCTTC TGGAGTCACA 
GGACCACCAG GACCTCCAGG ATACTGTGAC TCCTCTCAGT GCGGCATGCC TTACAACGGA 
CAAGGCTACC CAGGTTTTGC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL12A1A ( XM_002665259.5 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_002665305.3 Danio rerio collagen, type XII, alpha 1a (col12a1a), transcript variant X2, mRNA.

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CloneID	ODa22912
Clone ID Related Accession (Same CDS sequence)	XM_002665259.6 , XM_002665259.5
Accession Version	XM_002665259.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	collagen alpha-1(XII) chain isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_002665259.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATCA GGTTGTCTTT GGCCGCTGCT GCGCTCTTGA CTCTAGTACT CAGTTCAGTA 
GAGGCTCAAG TGGAGCCTCC GTCAGACTTA AAATTTAAAA TCCTGAATGA AAACACAGTG 
CAAATGTCAT GGAGGAAGCC CTCGTCTCAG ATACAGGGCT ACAGAATACA GGTCACGTCA 
GATACAGATG ATTCTAAAGA ATTGAGTCTT CCTGCCTCGT CCACAAGCAC ATCAATTACA 
GATCTAACTC CAGATGTAGA CTATTCAGTG TCAATCAATT CATATGATGG TGCTGAAGAA 
AGTATCCCTA TCCTTGGACA AATCACAATT CAATCCAGCA ACACAAGTGG ACCAAGGAGG 
CCACAGTCAG AGGCTGTCAA GTGTTCAGTG AGTGCCGTTG CGGATCTGGT TTTCCTGGTG 
GACGGCTCTT GGAGTGTGGG AAGAGAAAAC TTTAGATTTA TCCGGAGTTT CATTGGGGCC 
ATGGCCGGGG CCTTCGACAT TGAAGAGGAT AAGACCAGAG TGGCTGTGGT TCAGTACAGC 
TCGGACACCA GGACAGAGTT CAGTCTAAAC ACACATTTCA GACGACCCGA TGTCCTGCGA 
GCCATTAAAA ATCTGCCGTA CAAGGGTGGA AATACCATGA CGGGTGATGC AATGGACTAT 
CTGGTGAAAA ACACGTTCAC TCAGGCGGCT GGAGCCAGGA AAGGCTTCCC AAAAGTGGCC 
ATGATCATCA CAGATGGAAA ATCTCAAGAC CCCGTAGAGG AGTACGCCGA ACGGTTGAGG 
AACATCGGAG TGGAGATCTT TGTGCTGGGT ATCAAAGGAG CAGATGAGGA CGAGCTGAAG 
GAGATCGCCT CCAGACCCCA TAGCAAGCAT GTCTATAACG TCCCCAACTT CGACATGATC 
AATCAGGTCC AGAAGGCGCT CATCACTGAA GTTTGCTCTG GCGTGGAGGA GCAGCTAAAT 
TCCCTTGTAA GCGGGGAGGA AGTTGTCGAA CCAGCTTCCA ACCTCAGAGT GACAGATATC 
TCCTCCAAAT CCATGAGAAT TGCGTGGGAT TCATCTCCTG GTGATATCAC AGGCTATAAG 
CTGCAGGTTT TTCCCACTAT TGCGAGCGCC AAAAAGCAGG AGCTCTACAC TGGGCCCACG 
ATGACCTTGG CCAATGTCAG AGACCTGACG CCTGAGATGG AGTATGAGAT CAGTCTGTTT 
GCTCTCAAAG GTCTGACTCC TAGTGTGCCG CAAGTTGTCA CTGAGAAAAC TCAGCCAGTG 
AAAGTATTTA CAGAGTGTTC CCTAGAGGTG GATGTCCAAG CTGACATTGT CCTCCTGGTG 
GATGGGTCAT ATAGTATAGG ACTGCCTAAC TTTGCGAAGG TTAGAGCTTT CCTCGAAGTA 
CTTGTGAATT CTTTTGACAT CGGACCTAAC AAAGTGCAGA TCAGCTTGGT TCAGTACAGC 
AGGGATCCTC ACACTGAGTT CGCCTTGAAT AAATTCGATG ACAATGCAGC CATGGTCAAG 
GCGGTCCGCA CCTTCCCCTA CCGAGGTGGA TCCACCAACA CCGGCAAGGC CATGACATAT 
GTGAGGGAAA AGATCTTTGT TTCGGGCCGT GGCGCTCGTG ATAACGTCCC CCGAGTCATG 
GTGCTTATCA CAGACGGCAA GTCCTCAGAT TCTTTCAAAG ACCCCGCCAA CAAACTGAGG 
GATACAGATG TGGAGATTTT TGCAGTGGGA GTAAAAGATG CCGTACGATC TGAGCTGGAG 
GCTATCGCCA ATGTTCCTGC TGATAACCAT GTGTTTGAGG TGGAGGACTT TGATGCTTTC 
GAGCGGATCT CCAAGGAGCT GACCGCGTCC ATCTGCCTCA GAATTGAGCA GGAGCTCCAT 
AACATCAGGA AAAGAAGTTT GCTTCCCCCA AAGTCACTGA GCTTCTCTGA GGTGACCTCC 
AGGAGTTTCA GGACCTCATG GGTTTCTGAT GGAGAGGATA TCCTGTCTTT TCTGGTGCGC 
TTCAGGCCAG CGGCGGACCT AACTGGAGAC TACATCTCTC TGGTGGTTCC CCATGACATC 
ACCAGTACAA TTCTGCCCAA TCTCACGCCA CTCACCACCT ACGAAGTTAA CGTCATTGCT 
CAGTATGATA AAGGAGACAG CTTTCCTTTG ACGGGCGAGG AGACCACTTT AGAAGAGTTG 
GGTTCTGTTC GTAACCTGAT GGTGAGCGAG GAGGCTGTGG ACCGCTTTCG GGTGTCGTGG 
AGAGCTGCTC CAGGATTAGT GCTGAGGTAC AGACTGCTCT ACGAGCCTGT GAGCGGAGGG 
GATAAACTGG AAGCAGAGAC TGATGGGAAT CAGGTCACCA CTGTGCTCAA TGAACTACTA 
CCGGTCACTA CTTACCGGGT CACCGTTTAC CCTGAGTACG CAAGTGGAAG GGGTCCCTCC 
ATGGACACTG AAGGCACCAC CAAAGAAGTT CGAGGTTCTC CACGCGACTT AAGGGTCTTC 
AATGAGACGG TGTCTAGCAT GAGGGTCTCG TGGCGGGCTG CCCCAGGCAA CGTGCTTCAA 
TATCAGGTGG CCTACAAACC TGACGGTGGG GAGAGGAAAG AAATAAGTGT TAAAGGAGAG 
ACAACAACTG CTGTGCTGAA AAATCTGCTG CCGGACACGG AGTATGAGCT GTTTGTTAGC 
GCACGTTACG TCTCTGGACT TGGCGCACCA CTTATGGGAA CAGGCACCAC GCTGGAAGAA 
CTTGGTTCAC CTTCGAATCT GGTAACTTCA GACGTCACTG AGAACAGTTT TGTAGCATCC 
TGGACCGCCG CCCCTGGAAA CGTCCGTCTT TATCGGGTCA CCTGGAAATC CCTCTTTTCA 
GAGGAAGCTG GCGAGAAGAC TGTCCCCGGG GACGTAACCA CCACTGTTCT GGACGGGCTG 
ACACCAGAGA CCCGCTACAA GGTTTCTGTC TACGCTGTCT ACAGCCGTGC GGAGGGGGCG 
CCACTGCACG GAGAGGAGAC CACAGATGTG TCGGCCGCTA CCAGAGCTCT TACGGTGTCA 
GAAGAAACAG AGAGGACCAT GAAGGTGACA TGGATACCAG CACCTGGCAA TGTTGTCAAT 
TACAGGGTAT CATACAAACC TCAGGGTGAT TTACGCCAAA TGGCCTCCAA AGCACCAGGA 
GGCACCAATT CTATAGTCCT GAGACGCCTG ATCCCCCAGA CCACTTACGA TATCACAGTG 
GTTCCCGTCT ACAAGTTTGG GGATGGAAGA CAAAGGCAAG GAGAAGGAAC TACATTGTCT 
CAATATAAAG CTCCCAGAAA CTTGCAAACT TCGGAGCCCA CTAAAACCAG TTTCAGGGTG 
ACCTGGGATC ATGCTCCTGG AGATGTAAAA GGCTACAAGG TCACTTTCCA TCCCACCGGA 
AAGGAAGTGG ACCTGGGAGA GTTACTCGTA GGCCCCTATG ACAACACTGT GGTTCTGGAG 
GAACTAAGGG CCGGCACTAA GTATACGGTA AATGTGTTTG GTATGTTTGA TGGAGGAGAG 
AGTATGCCAT TGGCTGGAGA GGAGAAGACC ACCCTCTCTG ACGCAGCTTC TGAAAACCTC 
CCTTATACAC CTTCAGATGC CCAGTGTAAA ACCACGGCCC AAGCGGATAT CGTCCTGCTT 
GTTGATGGAT CTTGGAGCAT TGGTCGCCTG AACTTCAAGA CCATACGCAA TTTCATTGCT 
CGCATGGTGG GAGTCTTCGA CATCGGCCCC GATCGTGTCC AGATTGGTCT GGCACAGTAC 
AGCGGAGACC CGAAGACTGA GTGGCATCTC AATGCTCACA GAACCAGGAC TCAGCTGCTG 
GATGCGGTGG CCAATCTACC CTACAAAGGA GGAAACACTC TCACCGGCCT TGCTTTGAAT 
TACATCCTTC AGAATAACTT CAAGCCTAAC GTTGGCATGC GTCCAAACTC CAGAAAGATC 
GGCGTCCTGG TCACTGATGG CAAGTCCCAA GACGACATTG TGGCGAACTC ACAGAACCTG 
CGGGACCAAG GGATTGAACT GTACGCCATT GGTGTAAAGA ACGCAGATGA GAATGAGTTG 
AGAACCATTG CTTCCGATCC CGATGACATT CACATGTACA ACGTGGCTGA TTTCTCCTTC 
CTGCTGGACA TTGTGGATGA CCTCACAAAC AACCTGTGCA ACAGCGTCAA AGGTCCAGGA 
GGAGGACCAG AGCCACCCAC TGATCTGACC ACCTCAGAGG TCACACATTA CTCTTTCCGT 
GCCACTTGGG TGGCTCCTGA CGGGCCTGTG GAGAAGTACA GAGTGGAGTA TGTGAGCACC 
GCTGGAGGAG AACCACAGCA ACAGATGTTT GTGGACGGGA CGGTGACCAC TGTGGTTTTG 
GAAGGTCTCA CTCCGCTGAC CGAGTACATA GTAAAGGTTT ACTCAGTGGT GGGAGAGGAC 
AGCAGCGACC CACTCAGAGG CATAGAGACC ACATTGCCTC TCAGTGCTGT GAGGAGCATG 
AACATCTACA AAGAGCGCTC CACCACCATG AATGTGAGAT GGGACGCAGC CACAGGGGCC 
ACAGGCTACA TGCTTCTCTA CAAGGCCCTT AACGCCACTG AACCCTCTGT GGAGCAGGAG 
ATGCGAGTGA AAGGAATGGT CACTGACGTC CAGCTGAAGA AACTTCTCCC CAACACGGCC 
TACAGTGTTT CGCTCTTCGC CCTGCACGGC GATTACGCCA GTGAACCTCT GATTGATCAA 
GGGGTGACCC TGCCTCTTCC TCCATCCGGT GAGTTGAGGA TTACCGATGT GACCCACAGC 
ACCATGCAGC TGGACTGGGA CGCTGCGCCC GGCGCCGTCC GAAAGTACAT CATCACTTAC 
AAGCCAGAGG AGGGAGAGGC GAAGGAGCTT GAAGTTGACG GAGATGTAAC ATCTAGAGGC 
CTGGATAATC TCATCTCCCA GACTGAATAT GATGTGGCTG TGACTCCGGT TTATGATTCG 
GGCACTGGAA ACCCCATGCT TAACCAGGCC ATTACAGATG TGGTTCCTGC CCCGCAGAAC 
TTGAGGTTCT CGATGGTGAC CCAGACCAGT TTTAGGGTTC ACTGGGAGCA CGGCGCTCCT 
GATGTCTCCC TCTACCGTAT TGGCTGGAAA AAGAAAGGAA CGACCGAGTT TCAAAATGAT 
ATCCTGAGTA GTGAAGACAC TACATATGAT CTGGTGGACC TGGAGCCAGA CACACCTTAT 
GATGTTTCGG TCACTGCCAT TTACCCTGAT GAGTCTGAGA GCGAGGATCT GCTGGGCACT 
GAACGCACTT TGGCCAAGCT TGCTACACCA CCTCCCAGTG GTCCTCCTCA AAACCTCCAG 
GTGTTCGACG AGACCACCAC CTCCCTCACG ACTAAGTGGG ACCACGCGCC TGGACGAGTT 
CAGAACTACA AAATCACCTA CGTACCGCTT ACTGGAGGAC GATCACAGTC GACGCAGGTC 
GGAGGAAAGA AAAACACAGT GATCCTGCAG AAACTGACTC CAGACACTCC TTACTCCATC 
ACTGTTGCTG CGGTCTACGC CAGCGGAGAG GCCAGAGACA TTTCTGGCCA AGGAAAGACA 
TTGCCGCTGG GCAGTGTCAG GAATCTTCAG GTCACTGACC CCACAGTTAG CACTCTGAAT 
GTGCGCTGGG AGCCGGCGGA GGGAAGCGTT CGCCAGTACA GGATCTACTA CCAGCCCGTC 
GCAGGGGGCG CAGAGGACAT GGTCCAGGTT CCTGGAAGCA CCACCAGCAC CGTTCTGAAG 
AACCTGCAAT CTGACACTCA GTACAGAGTG ACGGTGGTGC CAGTCTACGC TCCTGGAGAG 
GGCAAGCGCA TGTCGGAGAC CGGAAAAACA CTGGTTCGGA CCCCTGCCAA GAACATCCAG 
GTTTACAACC CCACCACAAA CACCCTGAAC GTGCGCTGGG AGCCGGCCTC AGGACAGGTG 
CAGCAGTACC GCGTGGCTTA CGCCCCTCTC TCCGGGATCC GACCCTCTCA GTCGGTGCTG 
GTTCCAGGAA ACACCCACAC GGCTCTGCTG CAGCAGCTGG AATCAGACAC TGACTACTCT 
GTGAGTGTGG TGGCTCTGTA TGCTGATGGA GAGGGCTCGT CTGTGTCTGA TAATGGCAAA 
ACACTGCCCC GCAGTGGCCC CAGGAACCTG CGGGTTTATG ATCCCACCAC CAGCACGCTG 
TCGGTGGGCT GGGAACATGC AGAAGGGCCT GTGATTCAGT ACCGCATTGG CTACGCACCC 
ACCACTGGAG ATCCCATTGA AGAATTTACA ACTGTCCCAG GCAACCGTAA CTCGGTCATC 
CTGCAAAACC TGCAGCCAGA CACGCCGTAC AACATCAACG TAGAAGCCAT TTATCAAGAT 
GGCCCTGGAG GACGACTCAG TGGAGATGGA AAAACATTGG ATCTCCTTGG GCCTCGTAAC 
CTGCGTGTTT CAGACGAGTG GTACACACGT TTCCGAGTGT CCTGGGATCC TGCAGCCGCA 
CGGGTGAATG GATATAAACT GGTCTATACA CCAGTGGGCA CAACCGACAC CATGGAAGCC 
TTCGTTGGAG ACGTGACTTC ACACCACCTG AACAACCTCC AGCCTGGCAC CACATACGAC 
CTGAAGGTTT ACGCTCAGTA TGATGCGGGC TTGAGCGGCG CTCTGGTCGG ACAGGGAACC 
ACCCTGTATC TCAACGTGAC CGACCTCACT TCCTACAACG TGGGCTACGA CTCTTTCTGC 
ATCAGATGGA CTCCTCATCG AGCTGCCACC TCCTACAGAC TCAAAGTCAA CCCTTTCAAC 
CCATCTCACC GTGGAGCTCA GGAGGTCACG ATCACTGCGG CTGAGAGCCA GTACTGTTTT 
GACGGCCTGA CGCCAGACGC GCTATACAAC GCCACCGTCT ACACGCAGAC CCCCAACCGA 
GAGGGGCCCG GAGTCAGTGT CAAAGAGCGC ACACTGGTGA AGCCCACCGT CGCCCCAACA 
CTACCTCCAA CTCCTCCACC TCCAGCCACC ATTCCTTCTG CACTCGAGGT GTGCAAAGGT 
GCCAAGGCTG ACCTGGTGTT CCTGATCGAT GGCTCGTGGA GTATCGGAGA TGACAGCTTC 
GCTAAAGTCC GGCAGTTTGT GTTCAGCATG ATTGGGGCGT TTGATGTGGT CAGCCATGAG 
GGCATGCAGG TGTCTTTCGT GCAGTACAGC GACGACGCCA AAACTGAGTT CAAGCTCAAC 
ACCTACCACG ACAAGGGAAT GGTGCTGTCT GCTCTTCAGA TGGTCAATTA TAGAGGAGGA 
AACACCAAAA CAGGTGCTGC ACTGAAGCAT GTTGGAGAAA AAGTGTTCAC CTCGGACAAT 
GGCATGAGGA GAACCGTTCC CAAGGTACTG GTTGTGGTGA CCGATGGACG TTCTCAAGAT 
GACGTGAAGA AGAGCGCTGC TAAACTACAG CATGCAGGTT ACAGTGTGTT TGTGGTGGGT 
GTCGCTGACA TTGACGTTAA TGAACTGAGG AACATTGGAA GCAAGCCCAG TGACAGACAC 
GTCTTTATCG TCGACAACTT CGATGCTTTC GCCAAGATCC AAGACAACCT GATCACGTTC 
ATCTGTGAAA CGGCAACGTC CACATGCCCT TTGATTTACC TCAATGGATT CACATCACCA 
GGCTTCAGGA TGCTGGAAGC TTTCAACATC ACTGACAAGA CCTACGCAGG CATGAACGGC 
GTCTCTCTGG AAACCGGCTC CTTCAACAGC TTCATCGCAT ACAGACTCCA TAAGGACGCC 
TTCATTTCCC AGCCAACAAA GGAAATCCAT CCAGATGGTC TTCCTCCATC CTACACCCTC 
ATCCTCCTCT TCCGTCTGCT CCCGGACACC GGCAAGGATG CGTTTGATTT ATGGCAGATC 
TCAGATAAGA ACAATAACCC TGAGGTCGGG CTCACCATTG ACCCCTCCAG CAGAGTGATC 
TCCTTCTACA ATAAAGACAC TAGAGGGGAA ATTCAGAAAG TCTCCTTTGA TAACGACCAC 
GTGAAGAAGA TTTTCCACGG CAGTTTCCAC AAGCTTCACA TTTCTATTTC GCCGAAAAAT 
GTCAAACTCA ACATCGACTG CCAAGAGGTG GCTGAGAAAC AGATCAAAGC AGCCAACAAC 
GTCTCCACAG ATGGCTACGA AGTCCTTGGA AAAATGACAA AGTCTGGCGG TTCAAAGAAG 
CAGTCGGCCA CATTCCAGCT CCAGATGTTT GATATTGTCT GCAGTTTGGG CTGGACAAGT 
CGAGACAAAT GTTGTGATAT TCCAGCAATG CGAGATGAAG CCAAGTGTCC TGCTCTTCCT 
CACTCCTGCA CATGTGCTCA GGACAGTGTT GGTCCTCCAG GCCCAGCGGG TCCTCAAGGC 
AGTCCTGGTA CAAAAGGCCC ACGAGGCGAG AGAGGAGAGT CTGGTCCTAC GGGTCCAATG 
GGTCCACGTG GAGAGCAGGG TCTGCCAGGG CAGATGGGGC CACCGGGTCC ACAGGGCCCC 
AATGGACTAT CCCTTCCCGG AGAACCAGGG CGACCGGGAC CTAAGGGAGA CCCCGGTGAC 
TCAGGTTTAC CTGGACAGAG AGGCCCGGTT GGCGCTGCAG GTCCACTCGG CCCTGTTGGA 
CCCGCTGGAG CAAGAGGTCC ACCAGGAAGT GAAGGCCCAT CTGGACCAAG AGGTCCATCG 
GGACCAATGG GACCACCGGG AGCTCCCGGA ATGCCAGGAA TCACCGGAAA ACCAGGAAAA 
CCCGGAGACA CTGGCTTTCC TGGACCTGTT GGCATGAAAG GAGAGAAGGG TGAAAGAGGA 
GACACTGCGT CACAGAATAT GATGCGCTCT GTAGCGAGAC AAGTCTGCGA ACAGCTAGTC 
AGCAGACAAA TGAGCAGAAT TGATATGATG ATGAACCAGA TCCCCAGCAA CTACAGGAGT 
AACAGCCCCG GCCCCGCTGG ACCTCCAGGC CCACCTGGAA ACCAGGGACC CCATGGAGAG 
CCTGGTCAGC CCGGACCCAA TGGCTTCCCA GGAAATCCAG GTTTACCAGG CTCTCCTGGA 
GAAAGAGGAG TGCCTGGAGA GAAGGGTGAA CGAGGCAGCC CCGGCATTGG CACTCGAGGA 
CAGAGAGGAT TACCTGGACC TCCTGGGCCA CCAGGGCAGT CTCAGACCGG TCCCCCTGGT 
CCCTCAGGCT CACCCGGGCC CCGAGGACCA CCAGGACAAC ATGGTTCTTC TGGAGTCACA 
GGACCACCAG GACCTCCAGG ATACTGTGAC TCCTCTCAGT GCGGCATGCC TTACAACGGA 
CAAGGCTACC CAGGTTTTGC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002665305.3
CDS	140..9343
Translation	MKIRLSLAAAALLTLVLSSVEAQVEPPSDLKFKILNENTVQMSWRKPSSQIQGYRIQVTSDTDDSKELSLPASSTSTSITDLTPDVDYSVSINSYDGAEESIPILGQITIQSSNTSGPRRPQSEAVKCSVSAVADLVFLVDGSWSVGRENFRFIRSFIGAMAGAFDIEEDKTRVAVVQYSSDTRTEFSLNTHFRRPDVLRAIKNLPYKGGNTMTGDAMDYLVKNTFTQAAGARKGFPKVAMIITDGKSQDPVEEYAERLRNIGVEIFVLGIKGADEDELKEIASRPHSKHVYNVPNFDMINQVQKALITEVCSGVEEQLNSLVSGEEVVEPASNLRVTDISSKSMRIAWDSSPGDITGYKLQVFPTIASAKKQELYTGPTMTLANVRDLTPEMEYEISLFALKGLTPSVPQVVTEKTQPVKVFTECSLEVDVQADIVLLVDGSYSIGLPNFAKVRAFLEVLVNSFDIGPNKVQISLVQYSRDPHTEFALNKFDDNAAMVKAVRTFPYRGGSTNTGKAMTYVREKIFVSGRGARDNVPRVMVLITDGKSSDSFKDPANKLRDTDVEIFAVGVKDAVRSELEAIANVPADNHVFEVEDFDAFERISKELTASICLRIEQELHNIRKRSLLPPKSLSFSEVTSRSFRTSWVSDGEDILSFLVRFRPAADLTGDYISLVVPHDITSTILPNLTPLTTYEVNVIAQYDKGDSFPLTGEETTLEELGSVRNLMVSEEAVDRFRVSWRAAPGLVLRYRLLYEPVSGGDKLEAETDGNQVTTVLNELLPVTTYRVTVYPEYASGRGPSMDTEGTTKEVRGSPRDLRVFNETVSSMRVSWRAAPGNVLQYQVAYKPDGGERKEISVKGETTTAVLKNLLPDTEYELFVSARYVSGLGAPLMGTGTTLEELGSPSNLVTSDVTENSFVASWTAAPGNVRLYRVTWKSLFSEEAGEKTVPGDVTTTVLDGLTPETRYKVSVYAVYSRAEGAPLHGEETTDVSAATRALTVSEETERTMKVTWIPAPGNVVNYRVSYKPQGDLRQMASKAPGGTNSIVLRRLIPQTTYDITVVPVYKFGDGRQRQGEGTTLSQYKAPRNLQTSEPTKTSFRVTWDHAPGDVKGYKVTFHPTGKEVDLGELLVGPYDNTVVLEELRAGTKYTVNVFGMFDGGESMPLAGEEKTTLSDAASENLPYTPSDAQCKTTAQADIVLLVDGSWSIGRLNFKTIRNFIARMVGVFDIGPDRVQIGLAQYSGDPKTEWHLNAHRTRTQLLDAVANLPYKGGNTLTGLALNYILQNNFKPNVGMRPNSRKIGVLVTDGKSQDDIVANSQNLRDQGIELYAIGVKNADENELRTIASDPDDIHMYNVADFSFLLDIVDDLTNNLCNSVKGPGGGPEPPTDLTTSEVTHYSFRATWVAPDGPVEKYRVEYVSTAGGEPQQQMFVDGTVTTVVLEGLTPLTEYIVKVYSVVGEDSSDPLRGIETTLPLSAVRSMNIYKERSTTMNVRWDAATGATGYMLLYKALNATEPSVEQEMRVKGMVTDVQLKKLLPNTAYSVSLFALHGDYASEPLIDQGVTLPLPPSGELRITDVTHSTMQLDWDAAPGAVRKYIITYKPEEGEAKELEVDGDVTSRGLDNLISQTEYDVAVTPVYDSGTGNPMLNQAITDVVPAPQNLRFSMVTQTSFRVHWEHGAPDVSLYRIGWKKKGTTEFQNDILSSEDTTYDLVDLEPDTPYDVSVTAIYPDESESEDLLGTERTLAKLATPPPSGPPQNLQVFDETTTSLTTKWDHAPGRVQNYKITYVPLTGGRSQSTQVGGKKNTVILQKLTPDTPYSITVAAVYASGEARDISGQGKTLPLGSVRNLQVTDPTVSTLNVRWEPAEGSVRQYRIYYQPVAGGAEDMVQVPGSTTSTVLKNLQSDTQYRVTVVPVYAPGEGKRMSETGKTLVRTPAKNIQVYNPTTNTLNVRWEPASGQVQQYRVAYAPLSGIRPSQSVLVPGNTHTALLQQLESDTDYSVSVVALYADGEGSSVSDNGKTLPRSGPRNLRVYDPTTSTLSVGWEHAEGPVIQYRIGYAPTTGDPIEEFTTVPGNRNSVILQNLQPDTPYNINVEAIYQDGPGGRLSGDGKTLDLLGPRNLRVSDEWYTRFRVSWDPAAARVNGYKLVYTPVGTTDTMEAFVGDVTSHHLNNLQPGTTYDLKVYAQYDAGLSGALVGQGTTLYLNVTDLTSYNVGYDSFCIRWTPHRAATSYRLKVNPFNPSHRGAQEVTITAAESQYCFDGLTPDALYNATVYTQTPNREGPGVSVKERTLVKPTVAPTLPPTPPPPATIPSALEVCKGAKADLVFLIDGSWSIGDDSFAKVRQFVFSMIGAFDVVSHEGMQVSFVQYSDDAKTEFKLNTYHDKGMVLSALQMVNYRGGNTKTGAALKHVGEKVFTSDNGMRRTVPKVLVVVTDGRSQDDVKKSAAKLQHAGYSVFVVGVADIDVNELRNIGSKPSDRHVFIVDNFDAFAKIQDNLITFICETATSTCPLIYLNGFTSPGFRMLEAFNITDKTYAGMNGVSLETGSFNSFIAYRLHKDAFISQPTKEIHPDGLPPSYTLILLFRLLPDTGKDAFDLWQISDKNNNPEVGLTIDPSSRVISFYNKDTRGEIQKVSFDNDHVKKIFHGSFHKLHISISPKNVKLNIDCQEVAEKQIKAANNVSTDGYEVLGKMTKSGGSKKQSATFQLQMFDIVCSLGWTSRDKCCDIPAMRDEAKCPALPHSCTCAQDSVGPPGPAGPQGSPGTKGPRGERGESGPTGPMGPRGEQGLPGQMGPPGPQGPNGLSLPGEPGRPGPKGDPGDSGLPGQRGPVGAAGPLGPVGPAGARGPPGSEGPSGPRGPSGPMGPPGAPGMPGITGKPGKPGDTGFPGPVGMKGEKGERGDTASQNMMRSVARQVCEQLVSRQMSRIDMMMNQIPSNYRSNSPGPAGPPGPPGNQGPHGEPGQPGPNGFPGNPGLPGSPGERGVPGEKGERGSPGIGTRGQRGLPGPPGPPGQSQTGPPGPSGSPGPRGPPGQHGSSGVTGPPGPPGYCDSSQCGMPYNGQGYPGFA

Target ORF information:

RefSeq Version	XM_002665259.5
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio collagen, type XII, alpha 1a (col12a1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002665259.5

ORF Insert Sequence:

ATGAAGATCA GGTTGTCTTT GGCCGCTGCT GCGCTCTTGA CTCTAGTACT CAGTTCAGTA 
GAGGCTCAAG TGGAGCCTCC GTCAGACTTA AAATTTAAAA TCCTGAATGA AAACACAGTG 
CAAATGTCAT GGAGGAAGCC CTCGTCTCAG ATACAGGGCT ACAGAATACA GGTCACGTCA 
GATACAGATG ATTCTAAAGA ATTGAGTCTT CCTGCCTCGT CCACAAGCAC ATCAATTACA 
GATCTAACTC CAGATGTAGA CTATTCAGTG TCAATCAATT CATATGATGG TGCTGAAGAA 
AGTATCCCTA TCCTTGGACA AATCACAATT CAATCCAGCA ACACAAGTGG ACCAAGGAGG 
CCACAGTCAG AGGCTGTCAA GTGTTCAGTG AGTGCCGTTG CGGATCTGGT TTTCCTGGTG 
GACGGCTCTT GGAGTGTGGG AAGAGAAAAC TTTAGATTTA TCCGGAGTTT CATTGGGGCC 
ATGGCCGGGG CCTTCGACAT TGAAGAGGAT AAGACCAGAG TGGCTGTGGT TCAGTACAGC 
TCGGACACCA GGACAGAGTT CAGTCTAAAC ACACATTTCA GACGACCCGA TGTCCTGCGA 
GCCATTAAAA ATCTGCCGTA CAAGGGTGGA AATACCATGA CGGGTGATGC AATGGACTAT 
CTGGTGAAAA ACACGTTCAC TCAGGCGGCT GGAGCCAGGA AAGGCTTCCC AAAAGTGGCC 
ATGATCATCA CAGATGGAAA ATCTCAAGAC CCCGTAGAGG AGTACGCCGA ACGGTTGAGG 
AACATCGGAG TGGAGATCTT TGTGCTGGGT ATCAAAGGAG CAGATGAGGA CGAGCTGAAG 
GAGATCGCCT CCAGACCCCA TAGCAAGCAT GTCTATAACG TCCCCAACTT CGACATGATC 
AATCAGGTCC AGAAGGCGCT CATCACTGAA GTTTGCTCTG GCGTGGAGGA GCAGCTAAAT 
TCCCTTGTAA GCGGGGAGGA AGTTGTCGAA CCAGCTTCCA ACCTCAGAGT GACAGATATC 
TCCTCCAAAT CCATGAGAAT TGCGTGGGAT TCATCTCCTG GTGATATCAC AGGCTATAAG 
CTGCAGGTTT TTCCCACTAT TGCGAGCGCC AAAAAGCAGG AGCTCTACAC TGGGCCCACG 
ATGACCTTGG CCAATGTCAG AGACCTGACG CCTGAGATGG AGTATGAGAT CAGTCTGTTT 
GCTCTCAAAG GTCTGACTCC TAGTGTGCCG CAAGTTGTCA CTGAGAAAAC TCAGCCAGTG 
AAAGTATTTA CAGAGTGTTC CCTAGAGGTG GATGTCCAAG CTGACATTGT CCTCCTGGTG 
GATGGGTCAT ATAGTATAGG ACTGCCTAAC TTTGCGAAGG TTAGAGCTTT CCTCGAAGTA 
CTTGTGAATT CTTTTGACAT CGGACCTAAC AAAGTGCAGA TCAGCTTGGT TCAGTACAGC 
AGGGATCCTC ACACTGAGTT CGCCTTGAAT AAATTCGATG ACAATGCAGC CATGGTCAAG 
GCGGTCCGCA CCTTCCCCTA CCGAGGTGGA TCCACCAACA CCGGCAAGGC CATGACATAT 
GTGAGGGAAA AGATCTTTGT TTCGGGCCGT GGCGCTCGTG ATAACGTCCC CCGAGTCATG 
GTGCTTATCA CAGACGGCAA GTCCTCAGAT TCTTTCAAAG ACCCCGCCAA CAAACTGAGG 
GATACAGATG TGGAGATTTT TGCAGTGGGA GTAAAAGATG CCGTACGATC TGAGCTGGAG 
GCTATCGCCA ATGTTCCTGC TGATAACCAT GTGTTTGAGG TGGAGGACTT TGATGCTTTC 
GAGCGGATCT CCAAGGAGCT GACCGCGTCC ATCTGCCTCA GAATTGAGCA GGAGCTCCAT 
AACATCAGGA AAAGAAGTTT GCTTCCCCCA AAGTCACTGA GCTTCTCTGA GGTGACCTCC 
AGGAGTTTCA GGACCTCATG GGTTTCTGAT GGAGAGGATA TCCTGTCTTT TCTGGTGCGC 
TTCAGGCCAG CGGCGGACCT AACTGGAGAC TACATCTCTC TGGTGGTTCC CCATGACATC 
ACCAGTACAA TTCTGCCCAA TCTCACGCCA CTCACCACCT ACGAAGTTAA CGTCATTGCT 
CAGTATGATA AAGGAGACAG CTTTCCTTTG ACGGGCGAGG AGACCACTTT AGAAGAGTTG 
GGTTCTGTTC GTAACCTGAT GGTGAGCGAG GAGGCTGTGG ACCGCTTTCG GGTGTCGTGG 
AGAGCTGCTC CAGGATTAGT GCTGAGGTAC AGACTGCTCT ACGAGCCTGT GAGCGGAGGG 
GATAAACTGG AAGCAGAGAC TGATGGGAAT CAGGTCACCA CTGTGCTCAA TGAACTACTA 
CCGGTCACTA CTTACCGGGT CACCGTTTAC CCTGAGTACG CAAGTGGAAG GGGTCCCTCC 
ATGGACACTG AAGGCACCAC CAAAGAAGTT CGAGGTTCTC CACGCGACTT AAGGGTCTTC 
AATGAGACGG TGTCTAGCAT GAGGGTCTCG TGGCGGGCTG CCCCAGGCAA CGTGCTTCAA 
TATCAGGTGG CCTACAAACC TGACGGTGGG GAGAGGAAAG AAATAAGTGT TAAAGGAGAG 
ACAACAACTG CTGTGCTGAA AAATCTGCTG CCGGACACGG AGTATGAGCT GTTTGTTAGC 
GCACGTTACG TCTCTGGACT TGGCGCACCA CTTATGGGAA CAGGCACCAC GCTGGAAGAA 
CTTGGTTCAC CTTCGAATCT GGTAACTTCA GACGTCACTG AGAACAGTTT TGTAGCATCC 
TGGACCGCCG CCCCTGGAAA CGTCCGTCTT TATCGGGTCA CCTGGAAATC CCTCTTTTCA 
GAGGAAGCTG GCGAGAAGAC TGTCCCCGGG GACGTAACCA CCACTGTTCT GGACGGGCTG 
ACACCAGAGA CCCGCTACAA GGTTTCTGTC TACGCTGTCT ACAGCCGTGC GGAGGGGGCG 
CCACTGCACG GAGAGGAGAC CACAGATGTG TCGGCCGCTA CCAGAGCTCT TACGGTGTCA 
GAAGAAACAG AGAGGACCAT GAAGGTGACA TGGATACCAG CACCTGGCAA TGTTGTCAAT 
TACAGGGTAT CATACAAACC TCAGGGTGAT TTACGCCAAA TGGCCTCCAA AGCACCAGGA 
GGCACCAATT CTATAGTCCT GAGACGCCTG ATCCCCCAGA CCACTTACGA TATCACAGTG 
GTTCCCGTCT ACAAGTTTGG GGATGGAAGA CAAAGGCAAG GAGAAGGAAC TACATTGTCT 
CAATATAAAG CTCCCAGAAA CTTGCAAACT TCGGAGCCCA CTAAAACCAG TTTCAGGGTG 
ACCTGGGATC ATGCTCCTGG AGATGTAAAA GGCTACAAGG TCACTTTCCA TCCCACCGGA 
AAGGAAGTGG ACCTGGGAGA GTTACTCGTA GGCCCCTATG ACAACACTGT GGTTCTGGAG 
GAACTAAGGG CCGGCACTAA GTATACGGTA AATGTGTTTG GTATGTTTGA TGGAGGAGAG 
AGTATGCCAT TGGCTGGAGA GGAGAAGACC ACCCTCTCTG ACGCAGCTTC TGAAAACCTC 
CCTTATACAC CTTCAGATGC CCAGTGTAAA ACCACGGCCC AAGCGGATAT CGTCCTGCTT 
GTTGATGGAT CTTGGAGCAT TGGTCGCCTG AACTTCAAGA CCATACGCAA TTTCATTGCT 
CGCATGGTGG GAGTCTTCGA CATCGGCCCC GATCGTGTCC AGATTGGTCT GGCACAGTAC 
AGCGGAGACC CGAAGACTGA GTGGCATCTC AATGCTCACA GAACCAGGAC TCAGCTGCTG 
GATGCGGTGG CCAATCTACC CTACAAAGGA GGAAACACTC TCACCGGCCT TGCTTTGAAT 
TACATCCTTC AGAATAACTT CAAGCCTAAC GTTGGCATGC GTCCAAACTC CAGAAAGATC 
GGCGTCCTGG TCACTGATGG CAAGTCCCAA GACGACATTG TGGCGAACTC ACAGAACCTG 
CGGGACCAAG GGATTGAACT GTACGCCATT GGTGTAAAGA ACGCAGATGA GAATGAGTTG 
AGAACCATTG CTTCCGATCC CGATGACATT CACATGTACA ACGTGGCTGA TTTCTCCTTC 
CTGCTGGACA TTGTGGATGA CCTCACAAAC AACCTGTGCA ACAGCGTCAA AGGTCCAGGA 
GGAGGACCAG AGCCACCCAC TGATCTGACC ACCTCAGAGG TCACACATTA CTCTTTCCGT 
GCCACTTGGG TGGCTCCTGA CGGGCCTGTG GAGAAGTACA GAGTGGAGTA TGTGAGCACC 
GCTGGAGGAG AACCACAGCA ACAGATGTTT GTGGACGGGA CGGTGACCAC TGTGGTTTTG 
GAAGGTCTCA CTCCGCTGAC CGAGTACATA GTAAAGGTTT ACTCAGTGGT GGGAGAGGAC 
AGCAGCGACC CACTCAGAGG CATAGAGACC ACATTGCCTC TCAGTGCTGT GAGGAGCATG 
AACATCTACA AAGAGCGCTC CACCACCATG AATGTGAGAT GGGACGCAGC CACAGGGGCC 
ACAGGCTACA TGCTTCTCTA CAAGGCCCTT AACGCCACTG AACCCTCTGT GGAGCAGGAG 
ATGCGAGTGA AAGGAATGGT CACTGACGTC CAGCTGAAGA AACTTCTCCC CAACACGGCC 
TACAGTGTTT CGCTCTTCGC CCTGCACGGC GATTACGCCA GTGAACCTCT GATTGATCAA 
GGGGTGACCC TGCCTCTTCC TCCATCCGGT GAGTTGAGGA TTACCGATGT GACCCACAGC 
ACCATGCAGC TGGACTGGGA CGCTGCGCCC GGCGCCGTCC GAAAGTACAT CATCACTTAC 
AAGCCAGAGG AGGGAGAGGC GAAGGAGCTT GAAGTTGACG GAGATGTAAC ATCTAGAGGC 
CTGGATAATC TCATCTCCCA GACTGAATAT GATGTGGCTG TGACTCCGGT TTATGATTCG 
GGCACTGGAA ACCCCATGCT TAACCAGGCC ATTACAGATG TGGTTCCTGC CCCGCAGAAC 
TTGAGGTTCT CGATGGTGAC CCAGACCAGT TTTAGGGTTC ACTGGGAGCA CGGCGCTCCT 
GATGTCTCCC TCTACCGTAT TGGCTGGAAA AAGAAAGGAA CGACCGAGTT TCAAAATGAT 
ATCCTGAGTA GTGAAGACAC TACATATGAT CTGGTGGACC TGGAGCCAGA CACACCTTAT 
GATGTTTCGG TCACTGCCAT TTACCCTGAT GAGTCTGAGA GCGAGGATCT GCTGGGCACT 
GAACGCACTT TGGCCAAGCT TGCTACACCA CCTCCCAGTG GTCCTCCTCA AAACCTCCAG 
GTGTTCGACG AGACCACCAC CTCCCTCACG ACTAAGTGGG ACCACGCGCC TGGACGAGTT 
CAGAACTACA AAATCACCTA CGTACCGCTT ACTGGAGGAC GATCACAGTC GACGCAGGTC 
GGAGGAAAGA AAAACACAGT GATCCTGCAG AAACTGACTC CAGACACTCC TTACTCCATC 
ACTGTTGCTG CGGTCTACGC CAGCGGAGAG GCCAGAGACA TTTCTGGCCA AGGAAAGACA 
TTGCCGCTGG GCAGTGTCAG GAATCTTCAG GTCACTGACC CCACAGTTAG CACTCTGAAT 
GTGCGCTGGG AGCCGGCGGA GGGAAGCGTT CGCCAGTACA GGATCTACTA CCAGCCCGTC 
GCAGGGGGCG CAGAGGACAT GGTCCAGGTT CCTGGAAGCA CCACCAGCAC CGTTCTGAAG 
AACCTGCAAT CTGACACTCA GTACAGAGTG ACGGTGGTGC CAGTCTACGC TCCTGGAGAG 
GGCAAGCGCA TGTCGGAGAC CGGAAAAACA CTGGTTCGGA CCCCTGCCAA GAACATCCAG 
GTTTACAACC CCACCACAAA CACCCTGAAC GTGCGCTGGG AGCCGGCCTC AGGACAGGTG 
CAGCAGTACC GCGTGGCTTA CGCCCCTCTC TCCGGGATCC GACCCTCTCA GTCGGTGCTG 
GTTCCAGGAA ACACCCACAC GGCTCTGCTG CAGCAGCTGG AATCAGACAC TGACTACTCT 
GTGAGTGTGG TGGCTCTGTA TGCTGATGGA GAGGGCTCGT CTGTGTCTGA TAATGGCAAA 
ACACTGCCCC GCAGTGGCCC CAGGAACCTG CGGGTTTATG ATCCCACCAC CAGCACGCTG 
TCGGTGGGCT GGGAACATGC AGAAGGGCCT GTGATTCAGT ACCGCATTGG CTACGCACCC 
ACCACTGGAG ATCCCATTGA AGAATTTACA ACTGTCCCAG GCAACCGTAA CTCGGTCATC 
CTGCAAAACC TGCAGCCAGA CACGCCGTAC AACATCAACG TAGAAGCCAT TTATCAAGAT 
GGCCCTGGAG GACGACTCAG TGGAGATGGA AAAACATTGG ATCTCCTTGG GCCTCGTAAC 
CTGCGTGTTT CAGACGAGTG GTACACACGT TTCCGAGTGT CCTGGGATCC TGCAGCCGCA 
CGGGTGAATG GATATAAACT GGTCTATACA CCAGTGGGCA CAACCGACAC CATGGAAGCC 
TTCGTTGGAG ACGTGACTTC ACACCACCTG AACAACCTCC AGCCTGGCAC CACATACGAC 
CTGAAGGTTT ACGCTCAGTA TGATGCGGGC TTGAGCGGCG CTCTGGTCGG ACAGGGAACC 
ACCCTGTATC TCAACGTGAC CGACCTCACT TCCTACAACG TGGGCTACGA CTCTTTCTGC 
ATCAGATGGA CTCCTCATCG AGCTGCCACC TCCTACAGAC TCAAAGTCAA CCCTTTCAAC 
CCATCTCACC GTGGAGCTCA GGAGGTCACG ATCACTGCGG CTGAGAGCCA GTACTGTTTT 
GACGGCCTGA CGCCAGACGC GCTATACAAC GCCACCGTCT ACACGCAGAC CCCCAACCGA 
GAGGGGCCCG GAGTCAGTGT CAAAGAGCGC ACACTGGTGA AGCCCACCGT CGCCCCAACA 
CTACCTCCAA CTCCTCCACC TCCAGCCACC ATTCCTTCTG CACTCGAGGT GTGCAAAGGT 
GCCAAGGCTG ACCTGGTGTT CCTGATCGAT GGCTCGTGGA GTATCGGAGA TGACAGCTTC 
GCTAAAGTCC GGCAGTTTGT GTTCAGCATG ATTGGGGCGT TTGATGTGGT CAGCCATGAG 
GGCATGCAGG TGTCTTTCGT GCAGTACAGC GACGACGCCA AAACTGAGTT CAAGCTCAAC 
ACCTACCACG ACAAGGGAAT GGTGCTGTCT GCTCTTCAGA TGGTCAATTA TAGAGGAGGA 
AACACCAAAA CAGGTGCTGC ACTGAAGCAT GTTGGAGAAA AAGTGTTCAC CTCGGACAAT 
GGCATGAGGA GAACCGTTCC CAAGGTACTG GTTGTGGTGA CCGATGGACG TTCTCAAGAT 
GACGTGAAGA AGAGCGCTGC TAAACTACAG CATGCAGGTT ACAGTGTGTT TGTGGTGGGT 
GTCGCTGACA TTGACGTTAA TGAACTGAGG AACATTGGAA GCAAGCCCAG TGACAGACAC 
GTCTTTATCG TCGACAACTT CGATGCTTTC GCCAAGATCC AAGACAACCT GATCACGTTC 
ATCTGTGAAA CGGCAACGTC CACATGCCCT TTGATTTACC TCAATGGATT CACATCACCA 
GGCTTCAGGA TGCTGGAAGC TTTCAACATC ACTGACAAGA CCTACGCAGG CATGAACGGC 
GTCTCTCTGG AAACCGGCTC CTTCAACAGC TTCATCGCAT ACAGACTCCA TAAGGACGCC 
TTCATTTCCC AGCCAACAAA GGAAATCCAT CCAGATGGTC TTCCTCCATC CTACACCCTC 
ATCCTCCTCT TCCGTCTGCT CCCGGACACC GGCAAGGATG CGTTTGATTT ATGGCAGATC 
TCAGATAAGA ACAATAACCC TGAGGTCGGG CTCACCATTG ACCCCTCCAG CAGAGTGATC 
TCCTTCTACA ATAAAGACAC TAGAGGGGAA ATTCAGAAAG TCTCCTTTGA TAACGACCAC 
GTGAAGAAGA TTTTCCACGG CAGTTTCCAC AAGCTTCACA TTTCTATTTC GCCGAAAAAT 
GTCAAACTCA ACATCGACTG CCAAGAGGTG GCTGAGAAAC AGATCAAAGC AGCCAACAAC 
GTCTCCACAG ATGGCTACGA AGTCCTTGGA AAAATGACAA AGTCTGGCGG TTCAAAGAAG 
CAGTCGGCCA CATTCCAGCT CCAGATGTTT GATATTGTCT GCAGTTTGGG CTGGACAAGT 
CGAGACAAAT GTTGTGATAT TCCAGCAATG CGAGATGAAG CCAAGTGTCC TGCTCTTCCT 
CACTCCTGCA CATGTGCTCA GGACAGTGTT GGTCCTCCAG GCCCAGCGGG TCCTCAAGGC 
AGTCCTGGTA CAAAAGGCCC ACGAGGCGAG AGAGGAGAGT CTGGTCCTAC GGGTCCAATG 
GGTCCACGTG GAGAGCAGGG TCTGCCAGGG CAGATGGGGC CACCGGGTCC ACAGGGCCCC 
AATGGACTAT CCCTTCCCGG AGAACCAGGG CGACCGGGAC CTAAGGGAGA CCCCGGTGAC 
TCAGGTTTAC CTGGACAGAG AGGCCCGGTT GGCGCTGCAG GTCCACTCGG CCCTGTTGGA 
CCCGCTGGAG CAAGAGGTCC ACCAGGAAGT GAAGGCCCAT CTGGACCAAG AGGTCCATCG 
GGACCAATGG GACCACCGGG AGCTCCCGGA ATGCCAGGAA TCACCGGAAA ACCAGGAAAA 
CCCGGAGACA CTGGCTTTCC TGGACCTGTT GGCATGAAAG GAGAGAAGGG TGAAAGAGGA 
GACACTGCGT CACAGAATAT GATGCGCTCT GTAGCGAGAC AAGTCTGCGA ACAGCTAGTC 
AGCAGACAAA TGAGCAGAAT TGATATGATG ATGAACCAGA TCCCCAGCAA CTACAGGAGT 
AACAGCCCCG GCCCCGCTGG ACCTCCAGGC CCACCTGGAA ACCAGGGACC CCATGGAGAG 
CCTGGTCAGC CCGGACCCAA TGGCTTCCCA GGAAATCCAG GTTTACCAGG CTCTCCTGGA 
GAAAGAGGAG TGCCTGGAGA GAAGGGTGAA CGAGGCAGCC CCGGCATTGG CACTCGAGGA 
CAGAGAGGAT TACCTGGACC TCCTGGGCCA CCAGGGCAGT CTCAGACCGG TCCCCCTGGT 
CCCTCAGGCT CACCCGGGCC CCGAGGACCA CCAGGACAAC ATGGTTCTTC TGGAGTCACA 
GGACCACCAG GACCTCCAGG ATACTGTGAC TCCTCTCAGT GCGGCATGCC TTACAACGGA 
CAAGGCTACC CAGGTTTTGC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL12A1A ( XM_009293296.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009291571.1 Danio rerio collagen, type XII, alpha 1a (col12a1a), transcript variant X1, mRNA.

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CloneID	ODa48772
Clone ID Related Accession (Same CDS sequence)	XM_009293296.2 , XM_009293296.3
Accession Version	XM_009293296.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	collagen alpha-1(XII) chain isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293296.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATCA GGTTGTCTTT GGCCGCTGCT GCGCTCTTGA CTCTAGTACT CAGTTCAGTA 
GAGGCTCAAG TGGAGCCTCC GTCAGACTTA AAATTTAAAA TCCTGAATGA AAACACAGTG 
CAAATGTCAT GGAGGAAGCC CTCGTCTCAG ATACAGGGCT ACAGAATACA GGTCACGTCA 
GATACAGATG ATTCTAAAGA ATTGAGTCTT CCTGCCTCGT CCACAAGCAC ATCAATTACA 
GATCTAACTC CAGATGTAGA CTATTCAGTG TCAATCAATT CATATGATGG TGCTGAAGAA 
AGTATCCCTA TCCTTGGACA AATCACAATT CAATCCAGCA ACACAAGTGG ACCAAGGAGG 
CCACAGTCAG AGGCTGTCAA GTGTTCAGTG AGTGCCGTTG CGGATCTGGT TTTCCTGGTG 
GACGGCTCTT GGAGTGTGGG AAGAGAAAAC TTTAGATTTA TCCGGAGTTT CATTGGGGCC 
ATGGCCGGGG CCTTCGACAT TGAAGAGGAT AAGACCAGAG TGGCTGTGGT TCAGTACAGC 
TCGGACACCA GGACAGAGTT CAGTCTAAAC ACACATTTCA GACGACCCGA TGTCCTGCGA 
GCCATTAAAA ATCTGCCGTA CAAGGGTGGA AATACCATGA CGGGTGATGC AATGGACTAT 
CTGGTGAAAA ACACGTTCAC TCAGGCGGCT GGAGCCAGGA AAGGCTTCCC AAAAGTGGCC 
ATGATCATCA CAGATGGAAA ATCTCAAGAC CCCGTAGAGG AGTACGCCGA ACGGTTGAGG 
AACATCGGAG TGGAGATCTT TGTGCTGGGT ATCAAAGGAG CAGATGAGGA CGAGCTGAAG 
GAGATCGCCT CCAGACCCCA TAGCAAGCAT GTCTATAACG TCCCCAACTT CGACATGATC 
AATCAGGTCC AGAAGGCGCT CATCACTGAA GTTTGCTCTG GCGTGGAGGA GCAGCTAAAT 
TCCCTTGTAA GCGGGGAGGA AGTTGTCGAA CCAGCTTCCA ACCTCAGAGT GACAGATATC 
TCCTCCAAAT CCATGAGAAT TGCGTGGGAT TCATCTCCTG GTGATATCAC AGGCTATAAG 
CTGCAGGTTT TTCCCACTAT TGCGAGCGCC AAAAAGCAGG AGCTCTACAC TGGGCCCACG 
ATGACCTTGG CCAATGTCAG AGACCTGACG CCTGAGATGG AGTATGAGAT CAGTCTGTTT 
GCTCTCAAAG GTCTGACTCC TAGTGTGCCG CAAGTTGTCA CTGAGAAAAC TCAGCCAGTG 
AAAGTATTTA CAGAGTGTTC CCTAGAGGTG GATGTCCAAG CTGACATTGT CCTCCTGGTG 
GATGGGTCAT ATAGTATAGG ACTGCCTAAC TTTGCGAAGG TTAGAGCTTT CCTCGAAGTA 
CTTGTGAATT CTTTTGACAT CGGACCTAAC AAAGTGCAGA TCAGCTTGGT TCAGTACAGC 
AGGGATCCTC ACACTGAGTT CGCCTTGAAT AAATTCGATG ACAATGCAGC CATGGTCAAG 
GCGGTCCGCA CCTTCCCCTA CCGAGGTGGA TCCACCAACA CCGGCAAGGC CATGACATAT 
GTGAGGGAAA AGATCTTTGT TTCGGGCCGT GGCGCTCGTG ATAACGTCCC CCGAGTCATG 
GTGCTTATCA CAGACGGCAA GTCCTCAGAT TCTTTCAAAG ACCCCGCCAA CAAACTGAGG 
GATACAGATG TGGAGATTTT TGCAGTGGGA GTAAAAGATG CCGTACGATC TGAGCTGGAG 
GCTATCGCCA ATGTTCCTGC TGATAACCAT GTGTTTGAGG TGGAGGACTT TGATGCTTTC 
GAGCGGATCT CCAAGGAGCT GACCGCGTCC ATCTGCCTCA GAATTGAGCA GGAGCTCCAT 
AACATCAGGA AAAGAAGTTT GCTTCCCCCA AAGTCACTGA GCTTCTCTGA GGTGACCTCC 
AGGAGTTTCA GGACCTCATG GGTTTCTGAT GGAGAGGATA TCCTGTCTTT TCTGGTGCGC 
TTCAGGCCAG CGGCGGACCT AACTGGAGAC TACATCTCTC TGGTGGTTCC CCATGACATC 
ACCAGTACAA TTCTGCCCAA TCTCACGCCA CTCACCACCT ACGAAGTTAA CGTCATTGCT 
CAGTATGATA AAGGAGACAG CTTTCCTTTG ACGGGCGAGG AGACCACTTT AGAAGAGTTG 
GGTTCTGTTC GTAACCTGAT GGTGAGCGAG GAGGCTGTGG ACCGCTTTCG GGTGTCGTGG 
AGAGCTGCTC CAGGATTAGT GCTGAGGTAC AGACTGCTCT ACGAGCCTGT GAGCGGAGGG 
GATAAACTGG AAGCAGAGAC TGATGGGAAT CAGGTCACCA CTGTGCTCAA TGAACTACTA 
CCGGTCACTA CTTACCGGGT CACCGTTTAC CCTGAGTACG CAAGTGGAAG GGGTCCCTCC 
ATGGACACTG AAGGCACCAC CAAAGAAGTT CGAGGTTCTC CACGCGACTT AAGGGTCTTC 
AATGAGACGG TGTCTAGCAT GAGGGTCTCG TGGCGGGCTG CCCCAGGCAA CGTGCTTCAA 
TATCAGGTGG CCTACAAACC TGACGGTGGG GAGAGGAAAG AAATAAGTGT TAAAGGAGAG 
ACAACAACTG CTGTGCTGAA AAATCTGCTG CCGGACACGG AGTATGAGCT GTTTGTTAGC 
GCACGTTACG TCTCTGGACT TGGCGCACCA CTTATGGGAA CAGGCACCAC GCTGGAAGAA 
CTTGGTTCAC CTTCGAATCT GGTAACTTCA GACGTCACTG AGAACAGTTT TGTAGCATCC 
TGGACCGCCG CCCCTGGAAA CGTCCGTCTT TATCGGGTCA CCTGGAAATC CCTCTTTTCA 
GAGGAAGCTG GCGAGAAGAC TGTCCCCGGG GACGTAACCA CCACTGTTCT GGACGGGCTG 
ACACCAGAGA CCCGCTACAA GGTTTCTGTC TACGCTGTCT ACAGCCGTGC GGAGGGGGCG 
CCACTGCACG GAGAGGAGAC CACAGATGTG TCGGCCGCTA CCAGAGCTCT TACGGTGTCA 
GAAGAAACAG AGAGGACCAT GAAGGTGACA TGGATACCAG CACCTGGCAA TGTTGTCAAT 
TACAGGGTAT CATACAAACC TCAGGGTGAT TTACGCCAAA TGGCCTCCAA AGCACCAGGA 
GGCACCAATT CTATAGTCCT GAGACGCCTG ATCCCCCAGA CCACTTACGA TATCACAGTG 
GTTCCCGTCT ACAAGTTTGG GGATGGAAGA CAAAGGCAAG GAGAAGGAAC TACATTGTCT 
CAATATAAAG CTCCCAGAAA CTTGCAAACT TCGGAGCCCA CTAAAACCAG TTTCAGGGTG 
ACCTGGGATC ATGCTCCTGG AGATGTAAAA GGCTACAAGG TCACTTTCCA TCCCACCGGA 
AAGGAAGTGG ACCTGGGAGA GTTACTCGTA GGCCCCTATG ACAACACTGT GGTTCTGGAG 
GAACTAAGGG CCGGCACTAA GTATACGGTA AATGTGTTTG GTATGTTTGA TGGAGGAGAG 
AGTATGCCAT TGGCTGGAGA GGAGAAGACC ACCCTCTCTG ACGCAGCTTC TGAAAACCTC 
CCTTATACAC CTTCAGATGC CCAGTGTAAA ACCACGGCCC AAGCGGATAT CGTCCTGCTT 
GTTGATGGAT CTTGGAGCAT TGGTCGCCTG AACTTCAAGA CCATACGCAA TTTCATTGCT 
CGCATGGTGG GAGTCTTCGA CATCGGCCCC GATCGTGTCC AGATTGGTCT GGCACAGTAC 
AGCGGAGACC CGAAGACTGA GTGGCATCTC AATGCTCACA GAACCAGGAC TCAGCTGCTG 
GATGCGGTGG CCAATCTACC CTACAAAGGA GGAAACACTC TCACCGGCCT TGCTTTGAAT 
TACATCCTTC AGAATAACTT CAAGCCTAAC GTTGGCATGC GTCCAAACTC CAGAAAGATC 
GGCGTCCTGG TCACTGATGG CAAGTCCCAA GACGACATTG TGGCGAACTC ACAGAACCTG 
CGGGACCAAG GGATTGAACT GTACGCCATT GGTGTAAAGA ACGCAGATGA GAATGAGTTG 
AGAACCATTG CTTCCGATCC CGATGACATT CACATGTACA ACGTGGCTGA TTTCTCCTTC 
CTGCTGGACA TTGTGGATGA CCTCACAAAC AACCTGTGCA ACAGCGTCAA AGGTCCAGGA 
GGAGGACCAG AGCCACCCAC TGATCTGACC ACCTCAGAGG TCACACATTA CTCTTTCCGT 
GCCACTTGGG TGGCTCCTGA CGGGCCTGTG GAGAAGTACA GAGTGGAGTA TGTGAGCACC 
GCTGGAGGAG AACCACAGCA ACAGATGTTT GTGGACGGGA CGGTGACCAC TGTGGTTTTG 
GAAGGTCTCA CTCCGCTGAC CGAGTACATA GTAAAGGTTT ACTCAGTGGT GGGAGAGGAC 
AGCAGCGACC CACTCAGAGG CATAGAGACC ACATTGCCTC TCAGTGCTGT GAGGAGCATG 
AACATCTACA AAGAGCGCTC CACCACCATG AATGTGAGAT GGGACGCAGC CACAGGGGCC 
ACAGGCTACA TGCTTCTCTA CAAGGCCCTT AACGCCACTG AACCCTCTGT GGAGCAGGAG 
ATGCGAGTGA AAGGAATGGT CACTGACGTC CAGCTGAAGA AACTTCTCCC CAACACGGCC 
TACAGTGTTT CGCTCTTCGC CCTGCACGGC GATTACGCCA GTGAACCTCT GATTGATCAA 
GGGGTGACCC TGCCTCTTCC TCCATCCGGT GAGTTGAGGA TTACCGATGT GACCCACAGC 
ACCATGCAGC TGGACTGGGA CGCTGCGCCC GGCGCCGTCC GAAAGTACAT CATCACTTAC 
AAGCCAGAGG AGGGAGAGGC GAAGGAGCTT GAAGTTGACG GAGATGTAAC ATCTAGAGGC 
CTGGATAATC TCATCTCCCA GACTGAATAT GATGTGGCTG TGACTCCGGT TTATGATTCG 
GGCACTGGAA ACCCCATGCT TAACCAGGCC ATTACAGATG TGGTTCCTGC CCCGCAGAAC 
TTGAGGTTCT CGATGGTGAC CCAGACCAGT TTTAGGGTTC ACTGGGAGCA CGGCGCTCCT 
GATGTCTCCC TCTACCGTAT TGGCTGGAAA AAGAAAGGAA CGACCGAGTT TCAAAATGAT 
ATCCTGAGTA GTGAAGACAC TACATATGAT CTGGTGGACC TGGAGCCAGA CACACCTTAT 
GATGTTTCGG TCACTGCCAT TTACCCTGAT GAGTCTGAGA GCGAGGATCT GCTGGGCACT 
GAACGCACTT TGGCCAAGCT TGCTACACCA CCTCCCAGTG GTCCTCCTCA AAACCTCCAG 
GTGTTCGACG AGACCACCAC CTCCCTCACG ACTAAGTGGG ACCACGCGCC TGGACGAGTT 
CAGAACTACA AAATCACCTA CGTACCGCTT ACTGGAGGAC GATCACAGTC GACGCAGGTC 
GGAGGAAAGA AAAACACAGT GATCCTGCAG AAACTGACTC CAGACACTCC TTACTCCATC 
ACTGTTGCTG CGGTCTACGC CAGCGGAGAG GCCAGAGACA TTTCTGGCCA AGGAAAGACA 
TTGCCGCTGG GCAGTGTCAG GAATCTTCAG GTCACTGACC CCACAGTTAG CACTCTGAAT 
GTGCGCTGGG AGCCGGCGGA GGGAAGCGTT CGCCAGTACA GGATCTACTA CCAGCCCGTC 
GCAGGGGGCG CAGAGGACAT GGTCCAGGTT CCTGGAAGCA CCACCAGCAC CGTTCTGAAG 
AACCTGCAAT CTGACACTCA GTACAGAGTG ACGGTGGTGC CAGTCTACGC TCCTGGAGAG 
GGCAAGCGCA TGTCGGAGAC CGGAAAAACA CTGGTTCGGA CCCCTGCCAA GAACATCCAG 
GTTTACAACC CCACCACAAA CACCCTGAAC GTGCGCTGGG AGCCGGCCTC AGGACAGGTG 
CAGCAGTACC GCGTGGCTTA CGCCCCTCTC TCCGGGATCC GACCCTCTCA GTCGGTGCTG 
GTTCCAGGAA ACACCCACAC GGCTCTGCTG CAGCAGCTGG AATCAGACAC TGACTACTCT 
GTGAGTGTGG TGGCTCTGTA TGCTGATGGA GAGGGCTCGT CTGTGTCTGA TAATGGCAAA 
ACACTGCCCC GCAGTGGCCC CAGGAACCTG CGGGTTTATG ATCCCACCAC CAGCACGCTG 
TCGGTGGGCT GGGAACATGC AGAAGGGCCT GTGATTCAGT ACCGCATTGG CTACGCACCC 
ACCACTGGAG ATCCCATTGA AGAATTTACA ACTGTCCCAG GCAACCGTAA CTCGGTCATC 
CTGCAAAACC TGCAGCCAGA CACGCCGTAC AACATCAACG TAGAAGCCAT TTATCAAGAT 
GGCCCTGGAG GACGACTCAG TGGAGATGGA AAAACATTGG ATCTCCTTGG GCCTCGTAAC 
CTGCGTGTTT CAGACGAGTG GTACACACGT TTCCGAGTGT CCTGGGATCC TGCAGCCGCA 
CGGGTGAATG GATATAAACT GGTCTATACA CCAGTGGGCA CAACCGACAC CATGGAAGCC 
TTCGTTGGAG ACGTGACTTC ACACCACCTG AACAACCTCC AGCCTGGCAC CACATACGAC 
CTGAAGGTTT ACGCTCAGTA TGATGCGGGC TTGAGCGGCG CTCTGGTCGG ACAGGGAACC 
ACCCTGTATC TCAACGTGAC CGACCTCACT TCCTACAACG TGGGCTACGA CTCTTTCTGC 
ATCAGATGGA CTCCTCATCG AGCTGCCACC TCCTACAGAC TCAAAGTCAA CCCTTTCAAC 
CCATCTCACC GTGGAGCTCA GGAGGTCACG ATCACTGCGG CTGAGAGCCA GTACTGTTTT 
GACGGCCTGA CGCCAGACGC GCTATACAAC GCCACCGTCT ACACGCAGAC CCCCAACCGA 
GAGGGGCCCG GAGTCAGTGT CAAAGAGCGC ACACTGGTGA AGCCCACCGT CGCCCCAACA 
CTACCTCCAA CTCCTCCACC TCCAGCCACC ATTCCTTCTG CACTCGAGGT GTGCAAAGGT 
GCCAAGGCTG ACCTGGTGTT CCTGATCGAT GGCTCGTGGA GTATCGGAGA TGACAGCTTC 
GCTAAAGTCC GGCAGTTTGT GTTCAGCATG ATTGGGGCGT TTGATGTGGT CAGCCATGAG 
GGCATGCAGG TGTCTTTCGT GCAGTACAGC GACGACGCCA AAACTGAGTT CAAGCTCAAC 
ACCTACCACG ACAAGGGAAT GGTGCTGTCT GCTCTTCAGA TGGTCAATTA TAGAGGAGGA 
AACACCAAAA CAGGTGCTGC ACTGAAGCAT GTTGGAGAAA AAGTGTTCAC CTCGGACAAT 
GGCATGAGGA GAACCGTTCC CAAGGTACTG GTTGTGGTGA CCGATGGACG TTCTCAAGAT 
GACGTGAAGA AGAGCGCTGC TAAACTACAG CATGCAGGTT ACAGTGTGTT TGTGGTGGGT 
GTCGCTGACA TTGACGTTAA TGAACTGAGG AACATTGGAA GCAAGCCCAG TGACAGACAC 
GTCTTTATCG TCGACAACTT CGATGCTTTC GCCAAGATCC AAGACAACCT GATCACGTTC 
ATCTGTGAAA CGGCAACGTC CACATGCCCT TTGATTTACC TCAATGGATT CACATCACCA 
GGCTTCAGGA TGCTGGAAGC TTTCAACATC ACTGACAAGA CCTACGCAGG CATGAACGGC 
GTCTCTCTGG AAACCGGCTC CTTCAACAGC TTCATCGCAT ACAGACTCCA TAAGGACGCC 
TTCATTTCCC AGCCAACAAA GGAAATCCAT CCAGATGGTC TTCCTCCATC CTACACCCTC 
ATCCTCCTCT TCCGTCTGCT CCCGGACACC GGCAAGGATG CGTTTGATTT ATGGCAGATC 
TCAGATAAGA ACAATAACCC TGAGGTCGGG CTCACCATTG ACCCCTCCAG CAGAGTGATC 
TCCTTCTACA ATAAAGACAC TAGAGGGGAA ATTCAGAAAG TCTCCTTTGA TAACGACCAC 
GTGAAGAAGA TTTTCCACGG CAGTTTCCAC AAGCTTCACA TTTCTATTTC GCCGAAAAAT 
GTCAAACTCA ACATCGACTG CCAAGAGGTG GCTGAGAAAC AGATCAAAGC AGCCAACAAC 
GTCTCCACAG ATGGCTACGA AGTCCTTGGA AAAATGACAA AGTCTGGCGG TTCAAAGAAG 
CAGTCGGCCA CATTCCAGCT CCAGATGTTT GATATTGTCT GCAGTTTGGG CTGGACAAGT 
CGAGACAAAT GTTGTGATAT TCCAGCAATG CGAGATGAAG CCAAGTGTCC TGCTCTTCCT 
CACTCCTGCA CATGTGCTCA GGACAGTGTT GGTCCTCCAG GCCCAGCGGG TCCTCAAGGC 
AGTCCTGGTA CAAAAGGCCC ACGAGGCGAG AGAGGAGAGT CTGGTCCTAC GGGTCCAATG 
GGTCCACGTG GAGAGCAGGG TCTGCCAGGG CAGATGGGGC CACCGGGTCC ACAGGGCCCC 
AATGGACTAT CCCTTCCCGG AGAACCAGGG CGACCGGGAC CTAAGGGAGA CCCCGGTGAC 
TCAGGTTTAC CTGGACAGAG AGGCCCGGTT GGCGCTGCAG GTCCACTCGG CCCTGTTGGA 
CCCGCTGGAG CAAGAGGTCC ACCAGGAAGT GAAGGCCCAT CTGGACCAAG AGGTCCATCG 
GGACCAATGG GACCACCGGG AGCTCCCGGA ATGCCAGGAA TCACCGGAAA ACCAGGAAAA 
CCCGGAGACA CTGGCTTTCC TGGACCTGTT GGCATGAAAG GAGAGAAGGG TGAAAGAGGA 
GACACTGCGT CACAGAATAT GATGCGCTCT GTAGCGAGAC AAGTCTGCGA ACAGCTAGTC 
AGCAGACAAA TGAGCAGAAT TGATATGATG ATGAACCAGA TCCCCAGCAA CTACAGGAGT 
AACAGCCCCG GCCCCGCTGG ACCTCCAGGC CCACCTGGAA ACCAGGGACC CCATGGAGAG 
CCTGGTCAGC CCGGACCCAA TGGCTTCCCA GGAAATCCAG GTTTACCAGG CTCTCCTGGA 
GAAAGAGGAG TGCCTGGAGA GAAGGGTGAA CGAGGCAGCC CCGGCATTGG CACTCGAGGA 
CAGAGAGGAT TACCTGGACC TCCTGGGCCA CCAGGGCAGT CTCAGACCGG TCCCCCTGGT 
CCCTCAGGCT CACCCGGGCC CCGAGGACCA CCAGGACAAC ATGGTTCTTC TGGAGTCACA 
GGACCACCAG GACCTCCAGG ATACTGTGAC TCCTCTCAGT GCGGCATGCC TTACAACGGA 
CAAGGCTACC CAGGCCCCTA CCAGCCCGGG TCTGAAACCT ACGTAGTGCC CATACCAGCC 
GCGCCATCGG AAGATATCGA GACACAGTCG CCAGGATTGC GCTACCAGCG CGGAAAAAGG 
TCGCTATCAA GAAAGGAAGC CGAAAGAATC AAAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291571.1
CDS	140..9478
Translation	MKIRLSLAAAALLTLVLSSVEAQVEPPSDLKFKILNENTVQMSWRKPSSQIQGYRIQVTSDTDDSKELSLPASSTSTSITDLTPDVDYSVSINSYDGAEESIPILGQITIQSSNTSGPRRPQSEAVKCSVSAVADLVFLVDGSWSVGRENFRFIRSFIGAMAGAFDIEEDKTRVAVVQYSSDTRTEFSLNTHFRRPDVLRAIKNLPYKGGNTMTGDAMDYLVKNTFTQAAGARKGFPKVAMIITDGKSQDPVEEYAERLRNIGVEIFVLGIKGADEDELKEIASRPHSKHVYNVPNFDMINQVQKALITEVCSGVEEQLNSLVSGEEVVEPASNLRVTDISSKSMRIAWDSSPGDITGYKLQVFPTIASAKKQELYTGPTMTLANVRDLTPEMEYEISLFALKGLTPSVPQVVTEKTQPVKVFTECSLEVDVQADIVLLVDGSYSIGLPNFAKVRAFLEVLVNSFDIGPNKVQISLVQYSRDPHTEFALNKFDDNAAMVKAVRTFPYRGGSTNTGKAMTYVREKIFVSGRGARDNVPRVMVLITDGKSSDSFKDPANKLRDTDVEIFAVGVKDAVRSELEAIANVPADNHVFEVEDFDAFERISKELTASICLRIEQELHNIRKRSLLPPKSLSFSEVTSRSFRTSWVSDGEDILSFLVRFRPAADLTGDYISLVVPHDITSTILPNLTPLTTYEVNVIAQYDKGDSFPLTGEETTLEELGSVRNLMVSEEAVDRFRVSWRAAPGLVLRYRLLYEPVSGGDKLEAETDGNQVTTVLNELLPVTTYRVTVYPEYASGRGPSMDTEGTTKEVRGSPRDLRVFNETVSSMRVSWRAAPGNVLQYQVAYKPDGGERKEISVKGETTTAVLKNLLPDTEYELFVSARYVSGLGAPLMGTGTTLEELGSPSNLVTSDVTENSFVASWTAAPGNVRLYRVTWKSLFSEEAGEKTVPGDVTTTVLDGLTPETRYKVSVYAVYSRAEGAPLHGEETTDVSAATRALTVSEETERTMKVTWIPAPGNVVNYRVSYKPQGDLRQMASKAPGGTNSIVLRRLIPQTTYDITVVPVYKFGDGRQRQGEGTTLSQYKAPRNLQTSEPTKTSFRVTWDHAPGDVKGYKVTFHPTGKEVDLGELLVGPYDNTVVLEELRAGTKYTVNVFGMFDGGESMPLAGEEKTTLSDAASENLPYTPSDAQCKTTAQADIVLLVDGSWSIGRLNFKTIRNFIARMVGVFDIGPDRVQIGLAQYSGDPKTEWHLNAHRTRTQLLDAVANLPYKGGNTLTGLALNYILQNNFKPNVGMRPNSRKIGVLVTDGKSQDDIVANSQNLRDQGIELYAIGVKNADENELRTIASDPDDIHMYNVADFSFLLDIVDDLTNNLCNSVKGPGGGPEPPTDLTTSEVTHYSFRATWVAPDGPVEKYRVEYVSTAGGEPQQQMFVDGTVTTVVLEGLTPLTEYIVKVYSVVGEDSSDPLRGIETTLPLSAVRSMNIYKERSTTMNVRWDAATGATGYMLLYKALNATEPSVEQEMRVKGMVTDVQLKKLLPNTAYSVSLFALHGDYASEPLIDQGVTLPLPPSGELRITDVTHSTMQLDWDAAPGAVRKYIITYKPEEGEAKELEVDGDVTSRGLDNLISQTEYDVAVTPVYDSGTGNPMLNQAITDVVPAPQNLRFSMVTQTSFRVHWEHGAPDVSLYRIGWKKKGTTEFQNDILSSEDTTYDLVDLEPDTPYDVSVTAIYPDESESEDLLGTERTLAKLATPPPSGPPQNLQVFDETTTSLTTKWDHAPGRVQNYKITYVPLTGGRSQSTQVGGKKNTVILQKLTPDTPYSITVAAVYASGEARDISGQGKTLPLGSVRNLQVTDPTVSTLNVRWEPAEGSVRQYRIYYQPVAGGAEDMVQVPGSTTSTVLKNLQSDTQYRVTVVPVYAPGEGKRMSETGKTLVRTPAKNIQVYNPTTNTLNVRWEPASGQVQQYRVAYAPLSGIRPSQSVLVPGNTHTALLQQLESDTDYSVSVVALYADGEGSSVSDNGKTLPRSGPRNLRVYDPTTSTLSVGWEHAEGPVIQYRIGYAPTTGDPIEEFTTVPGNRNSVILQNLQPDTPYNINVEAIYQDGPGGRLSGDGKTLDLLGPRNLRVSDEWYTRFRVSWDPAAARVNGYKLVYTPVGTTDTMEAFVGDVTSHHLNNLQPGTTYDLKVYAQYDAGLSGALVGQGTTLYLNVTDLTSYNVGYDSFCIRWTPHRAATSYRLKVNPFNPSHRGAQEVTITAAESQYCFDGLTPDALYNATVYTQTPNREGPGVSVKERTLVKPTVAPTLPPTPPPPATIPSALEVCKGAKADLVFLIDGSWSIGDDSFAKVRQFVFSMIGAFDVVSHEGMQVSFVQYSDDAKTEFKLNTYHDKGMVLSALQMVNYRGGNTKTGAALKHVGEKVFTSDNGMRRTVPKVLVVVTDGRSQDDVKKSAAKLQHAGYSVFVVGVADIDVNELRNIGSKPSDRHVFIVDNFDAFAKIQDNLITFICETATSTCPLIYLNGFTSPGFRMLEAFNITDKTYAGMNGVSLETGSFNSFIAYRLHKDAFISQPTKEIHPDGLPPSYTLILLFRLLPDTGKDAFDLWQISDKNNNPEVGLTIDPSSRVISFYNKDTRGEIQKVSFDNDHVKKIFHGSFHKLHISISPKNVKLNIDCQEVAEKQIKAANNVSTDGYEVLGKMTKSGGSKKQSATFQLQMFDIVCSLGWTSRDKCCDIPAMRDEAKCPALPHSCTCAQDSVGPPGPAGPQGSPGTKGPRGERGESGPTGPMGPRGEQGLPGQMGPPGPQGPNGLSLPGEPGRPGPKGDPGDSGLPGQRGPVGAAGPLGPVGPAGARGPPGSEGPSGPRGPSGPMGPPGAPGMPGITGKPGKPGDTGFPGPVGMKGEKGERGDTASQNMMRSVARQVCEQLVSRQMSRIDMMMNQIPSNYRSNSPGPAGPPGPPGNQGPHGEPGQPGPNGFPGNPGLPGSPGERGVPGEKGERGSPGIGTRGQRGLPGPPGPPGQSQTGPPGPSGSPGPRGPPGQHGSSGVTGPPGPPGYCDSSQCGMPYNGQGYPGPYQPGSETYVVPIPAAPSEDIETQSPGLRYQRGKRSLSRKEAERIKT

Target ORF information:

RefSeq Version	XM_009293296.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio collagen, type XII, alpha 1a (col12a1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293296.2

ORF Insert Sequence:

ATGAAGATCA GGTTGTCTTT GGCCGCTGCT GCGCTCTTGA CTCTAGTACT CAGTTCAGTA 
GAGGCTCAAG TGGAGCCTCC GTCAGACTTA AAATTTAAAA TCCTGAATGA AAACACAGTG 
CAAATGTCAT GGAGGAAGCC CTCGTCTCAG ATACAGGGCT ACAGAATACA GGTCACGTCA 
GATACAGATG ATTCTAAAGA ATTGAGTCTT CCTGCCTCGT CCACAAGCAC ATCAATTACA 
GATCTAACTC CAGATGTAGA CTATTCAGTG TCAATCAATT CATATGATGG TGCTGAAGAA 
AGTATCCCTA TCCTTGGACA AATCACAATT CAATCCAGCA ACACAAGTGG ACCAAGGAGG 
CCACAGTCAG AGGCTGTCAA GTGTTCAGTG AGTGCCGTTG CGGATCTGGT TTTCCTGGTG 
GACGGCTCTT GGAGTGTGGG AAGAGAAAAC TTTAGATTTA TCCGGAGTTT CATTGGGGCC 
ATGGCCGGGG CCTTCGACAT TGAAGAGGAT AAGACCAGAG TGGCTGTGGT TCAGTACAGC 
TCGGACACCA GGACAGAGTT CAGTCTAAAC ACACATTTCA GACGACCCGA TGTCCTGCGA 
GCCATTAAAA ATCTGCCGTA CAAGGGTGGA AATACCATGA CGGGTGATGC AATGGACTAT 
CTGGTGAAAA ACACGTTCAC TCAGGCGGCT GGAGCCAGGA AAGGCTTCCC AAAAGTGGCC 
ATGATCATCA CAGATGGAAA ATCTCAAGAC CCCGTAGAGG AGTACGCCGA ACGGTTGAGG 
AACATCGGAG TGGAGATCTT TGTGCTGGGT ATCAAAGGAG CAGATGAGGA CGAGCTGAAG 
GAGATCGCCT CCAGACCCCA TAGCAAGCAT GTCTATAACG TCCCCAACTT CGACATGATC 
AATCAGGTCC AGAAGGCGCT CATCACTGAA GTTTGCTCTG GCGTGGAGGA GCAGCTAAAT 
TCCCTTGTAA GCGGGGAGGA AGTTGTCGAA CCAGCTTCCA ACCTCAGAGT GACAGATATC 
TCCTCCAAAT CCATGAGAAT TGCGTGGGAT TCATCTCCTG GTGATATCAC AGGCTATAAG 
CTGCAGGTTT TTCCCACTAT TGCGAGCGCC AAAAAGCAGG AGCTCTACAC TGGGCCCACG 
ATGACCTTGG CCAATGTCAG AGACCTGACG CCTGAGATGG AGTATGAGAT CAGTCTGTTT 
GCTCTCAAAG GTCTGACTCC TAGTGTGCCG CAAGTTGTCA CTGAGAAAAC TCAGCCAGTG 
AAAGTATTTA CAGAGTGTTC CCTAGAGGTG GATGTCCAAG CTGACATTGT CCTCCTGGTG 
GATGGGTCAT ATAGTATAGG ACTGCCTAAC TTTGCGAAGG TTAGAGCTTT CCTCGAAGTA 
CTTGTGAATT CTTTTGACAT CGGACCTAAC AAAGTGCAGA TCAGCTTGGT TCAGTACAGC 
AGGGATCCTC ACACTGAGTT CGCCTTGAAT AAATTCGATG ACAATGCAGC CATGGTCAAG 
GCGGTCCGCA CCTTCCCCTA CCGAGGTGGA TCCACCAACA CCGGCAAGGC CATGACATAT 
GTGAGGGAAA AGATCTTTGT TTCGGGCCGT GGCGCTCGTG ATAACGTCCC CCGAGTCATG 
GTGCTTATCA CAGACGGCAA GTCCTCAGAT TCTTTCAAAG ACCCCGCCAA CAAACTGAGG 
GATACAGATG TGGAGATTTT TGCAGTGGGA GTAAAAGATG CCGTACGATC TGAGCTGGAG 
GCTATCGCCA ATGTTCCTGC TGATAACCAT GTGTTTGAGG TGGAGGACTT TGATGCTTTC 
GAGCGGATCT CCAAGGAGCT GACCGCGTCC ATCTGCCTCA GAATTGAGCA GGAGCTCCAT 
AACATCAGGA AAAGAAGTTT GCTTCCCCCA AAGTCACTGA GCTTCTCTGA GGTGACCTCC 
AGGAGTTTCA GGACCTCATG GGTTTCTGAT GGAGAGGATA TCCTGTCTTT TCTGGTGCGC 
TTCAGGCCAG CGGCGGACCT AACTGGAGAC TACATCTCTC TGGTGGTTCC CCATGACATC 
ACCAGTACAA TTCTGCCCAA TCTCACGCCA CTCACCACCT ACGAAGTTAA CGTCATTGCT 
CAGTATGATA AAGGAGACAG CTTTCCTTTG ACGGGCGAGG AGACCACTTT AGAAGAGTTG 
GGTTCTGTTC GTAACCTGAT GGTGAGCGAG GAGGCTGTGG ACCGCTTTCG GGTGTCGTGG 
AGAGCTGCTC CAGGATTAGT GCTGAGGTAC AGACTGCTCT ACGAGCCTGT GAGCGGAGGG 
GATAAACTGG AAGCAGAGAC TGATGGGAAT CAGGTCACCA CTGTGCTCAA TGAACTACTA 
CCGGTCACTA CTTACCGGGT CACCGTTTAC CCTGAGTACG CAAGTGGAAG GGGTCCCTCC 
ATGGACACTG AAGGCACCAC CAAAGAAGTT CGAGGTTCTC CACGCGACTT AAGGGTCTTC 
AATGAGACGG TGTCTAGCAT GAGGGTCTCG TGGCGGGCTG CCCCAGGCAA CGTGCTTCAA 
TATCAGGTGG CCTACAAACC TGACGGTGGG GAGAGGAAAG AAATAAGTGT TAAAGGAGAG 
ACAACAACTG CTGTGCTGAA AAATCTGCTG CCGGACACGG AGTATGAGCT GTTTGTTAGC 
GCACGTTACG TCTCTGGACT TGGCGCACCA CTTATGGGAA CAGGCACCAC GCTGGAAGAA 
CTTGGTTCAC CTTCGAATCT GGTAACTTCA GACGTCACTG AGAACAGTTT TGTAGCATCC 
TGGACCGCCG CCCCTGGAAA CGTCCGTCTT TATCGGGTCA CCTGGAAATC CCTCTTTTCA 
GAGGAAGCTG GCGAGAAGAC TGTCCCCGGG GACGTAACCA CCACTGTTCT GGACGGGCTG 
ACACCAGAGA CCCGCTACAA GGTTTCTGTC TACGCTGTCT ACAGCCGTGC GGAGGGGGCG 
CCACTGCACG GAGAGGAGAC CACAGATGTG TCGGCCGCTA CCAGAGCTCT TACGGTGTCA 
GAAGAAACAG AGAGGACCAT GAAGGTGACA TGGATACCAG CACCTGGCAA TGTTGTCAAT 
TACAGGGTAT CATACAAACC TCAGGGTGAT TTACGCCAAA TGGCCTCCAA AGCACCAGGA 
GGCACCAATT CTATAGTCCT GAGACGCCTG ATCCCCCAGA CCACTTACGA TATCACAGTG 
GTTCCCGTCT ACAAGTTTGG GGATGGAAGA CAAAGGCAAG GAGAAGGAAC TACATTGTCT 
CAATATAAAG CTCCCAGAAA CTTGCAAACT TCGGAGCCCA CTAAAACCAG TTTCAGGGTG 
ACCTGGGATC ATGCTCCTGG AGATGTAAAA GGCTACAAGG TCACTTTCCA TCCCACCGGA 
AAGGAAGTGG ACCTGGGAGA GTTACTCGTA GGCCCCTATG ACAACACTGT GGTTCTGGAG 
GAACTAAGGG CCGGCACTAA GTATACGGTA AATGTGTTTG GTATGTTTGA TGGAGGAGAG 
AGTATGCCAT TGGCTGGAGA GGAGAAGACC ACCCTCTCTG ACGCAGCTTC TGAAAACCTC 
CCTTATACAC CTTCAGATGC CCAGTGTAAA ACCACGGCCC AAGCGGATAT CGTCCTGCTT 
GTTGATGGAT CTTGGAGCAT TGGTCGCCTG AACTTCAAGA CCATACGCAA TTTCATTGCT 
CGCATGGTGG GAGTCTTCGA CATCGGCCCC GATCGTGTCC AGATTGGTCT GGCACAGTAC 
AGCGGAGACC CGAAGACTGA GTGGCATCTC AATGCTCACA GAACCAGGAC TCAGCTGCTG 
GATGCGGTGG CCAATCTACC CTACAAAGGA GGAAACACTC TCACCGGCCT TGCTTTGAAT 
TACATCCTTC AGAATAACTT CAAGCCTAAC GTTGGCATGC GTCCAAACTC CAGAAAGATC 
GGCGTCCTGG TCACTGATGG CAAGTCCCAA GACGACATTG TGGCGAACTC ACAGAACCTG 
CGGGACCAAG GGATTGAACT GTACGCCATT GGTGTAAAGA ACGCAGATGA GAATGAGTTG 
AGAACCATTG CTTCCGATCC CGATGACATT CACATGTACA ACGTGGCTGA TTTCTCCTTC 
CTGCTGGACA TTGTGGATGA CCTCACAAAC AACCTGTGCA ACAGCGTCAA AGGTCCAGGA 
GGAGGACCAG AGCCACCCAC TGATCTGACC ACCTCAGAGG TCACACATTA CTCTTTCCGT 
GCCACTTGGG TGGCTCCTGA CGGGCCTGTG GAGAAGTACA GAGTGGAGTA TGTGAGCACC 
GCTGGAGGAG AACCACAGCA ACAGATGTTT GTGGACGGGA CGGTGACCAC TGTGGTTTTG 
GAAGGTCTCA CTCCGCTGAC CGAGTACATA GTAAAGGTTT ACTCAGTGGT GGGAGAGGAC 
AGCAGCGACC CACTCAGAGG CATAGAGACC ACATTGCCTC TCAGTGCTGT GAGGAGCATG 
AACATCTACA AAGAGCGCTC CACCACCATG AATGTGAGAT GGGACGCAGC CACAGGGGCC 
ACAGGCTACA TGCTTCTCTA CAAGGCCCTT AACGCCACTG AACCCTCTGT GGAGCAGGAG 
ATGCGAGTGA AAGGAATGGT CACTGACGTC CAGCTGAAGA AACTTCTCCC CAACACGGCC 
TACAGTGTTT CGCTCTTCGC CCTGCACGGC GATTACGCCA GTGAACCTCT GATTGATCAA 
GGGGTGACCC TGCCTCTTCC TCCATCCGGT GAGTTGAGGA TTACCGATGT GACCCACAGC 
ACCATGCAGC TGGACTGGGA CGCTGCGCCC GGCGCCGTCC GAAAGTACAT CATCACTTAC 
AAGCCAGAGG AGGGAGAGGC GAAGGAGCTT GAAGTTGACG GAGATGTAAC ATCTAGAGGC 
CTGGATAATC TCATCTCCCA GACTGAATAT GATGTGGCTG TGACTCCGGT TTATGATTCG 
GGCACTGGAA ACCCCATGCT TAACCAGGCC ATTACAGATG TGGTTCCTGC CCCGCAGAAC 
TTGAGGTTCT CGATGGTGAC CCAGACCAGT TTTAGGGTTC ACTGGGAGCA CGGCGCTCCT 
GATGTCTCCC TCTACCGTAT TGGCTGGAAA AAGAAAGGAA CGACCGAGTT TCAAAATGAT 
ATCCTGAGTA GTGAAGACAC TACATATGAT CTGGTGGACC TGGAGCCAGA CACACCTTAT 
GATGTTTCGG TCACTGCCAT TTACCCTGAT GAGTCTGAGA GCGAGGATCT GCTGGGCACT 
GAACGCACTT TGGCCAAGCT TGCTACACCA CCTCCCAGTG GTCCTCCTCA AAACCTCCAG 
GTGTTCGACG AGACCACCAC CTCCCTCACG ACTAAGTGGG ACCACGCGCC TGGACGAGTT 
CAGAACTACA AAATCACCTA CGTACCGCTT ACTGGAGGAC GATCACAGTC GACGCAGGTC 
GGAGGAAAGA AAAACACAGT GATCCTGCAG AAACTGACTC CAGACACTCC TTACTCCATC 
ACTGTTGCTG CGGTCTACGC CAGCGGAGAG GCCAGAGACA TTTCTGGCCA AGGAAAGACA 
TTGCCGCTGG GCAGTGTCAG GAATCTTCAG GTCACTGACC CCACAGTTAG CACTCTGAAT 
GTGCGCTGGG AGCCGGCGGA GGGAAGCGTT CGCCAGTACA GGATCTACTA CCAGCCCGTC 
GCAGGGGGCG CAGAGGACAT GGTCCAGGTT CCTGGAAGCA CCACCAGCAC CGTTCTGAAG 
AACCTGCAAT CTGACACTCA GTACAGAGTG ACGGTGGTGC CAGTCTACGC TCCTGGAGAG 
GGCAAGCGCA TGTCGGAGAC CGGAAAAACA CTGGTTCGGA CCCCTGCCAA GAACATCCAG 
GTTTACAACC CCACCACAAA CACCCTGAAC GTGCGCTGGG AGCCGGCCTC AGGACAGGTG 
CAGCAGTACC GCGTGGCTTA CGCCCCTCTC TCCGGGATCC GACCCTCTCA GTCGGTGCTG 
GTTCCAGGAA ACACCCACAC GGCTCTGCTG CAGCAGCTGG AATCAGACAC TGACTACTCT 
GTGAGTGTGG TGGCTCTGTA TGCTGATGGA GAGGGCTCGT CTGTGTCTGA TAATGGCAAA 
ACACTGCCCC GCAGTGGCCC CAGGAACCTG CGGGTTTATG ATCCCACCAC CAGCACGCTG 
TCGGTGGGCT GGGAACATGC AGAAGGGCCT GTGATTCAGT ACCGCATTGG CTACGCACCC 
ACCACTGGAG ATCCCATTGA AGAATTTACA ACTGTCCCAG GCAACCGTAA CTCGGTCATC 
CTGCAAAACC TGCAGCCAGA CACGCCGTAC AACATCAACG TAGAAGCCAT TTATCAAGAT 
GGCCCTGGAG GACGACTCAG TGGAGATGGA AAAACATTGG ATCTCCTTGG GCCTCGTAAC 
CTGCGTGTTT CAGACGAGTG GTACACACGT TTCCGAGTGT CCTGGGATCC TGCAGCCGCA 
CGGGTGAATG GATATAAACT GGTCTATACA CCAGTGGGCA CAACCGACAC CATGGAAGCC 
TTCGTTGGAG ACGTGACTTC ACACCACCTG AACAACCTCC AGCCTGGCAC CACATACGAC 
CTGAAGGTTT ACGCTCAGTA TGATGCGGGC TTGAGCGGCG CTCTGGTCGG ACAGGGAACC 
ACCCTGTATC TCAACGTGAC CGACCTCACT TCCTACAACG TGGGCTACGA CTCTTTCTGC 
ATCAGATGGA CTCCTCATCG AGCTGCCACC TCCTACAGAC TCAAAGTCAA CCCTTTCAAC 
CCATCTCACC GTGGAGCTCA GGAGGTCACG ATCACTGCGG CTGAGAGCCA GTACTGTTTT 
GACGGCCTGA CGCCAGACGC GCTATACAAC GCCACCGTCT ACACGCAGAC CCCCAACCGA 
GAGGGGCCCG GAGTCAGTGT CAAAGAGCGC ACACTGGTGA AGCCCACCGT CGCCCCAACA 
CTACCTCCAA CTCCTCCACC TCCAGCCACC ATTCCTTCTG CACTCGAGGT GTGCAAAGGT 
GCCAAGGCTG ACCTGGTGTT CCTGATCGAT GGCTCGTGGA GTATCGGAGA TGACAGCTTC 
GCTAAAGTCC GGCAGTTTGT GTTCAGCATG ATTGGGGCGT TTGATGTGGT CAGCCATGAG 
GGCATGCAGG TGTCTTTCGT GCAGTACAGC GACGACGCCA AAACTGAGTT CAAGCTCAAC 
ACCTACCACG ACAAGGGAAT GGTGCTGTCT GCTCTTCAGA TGGTCAATTA TAGAGGAGGA 
AACACCAAAA CAGGTGCTGC ACTGAAGCAT GTTGGAGAAA AAGTGTTCAC CTCGGACAAT 
GGCATGAGGA GAACCGTTCC CAAGGTACTG GTTGTGGTGA CCGATGGACG TTCTCAAGAT 
GACGTGAAGA AGAGCGCTGC TAAACTACAG CATGCAGGTT ACAGTGTGTT TGTGGTGGGT 
GTCGCTGACA TTGACGTTAA TGAACTGAGG AACATTGGAA GCAAGCCCAG TGACAGACAC 
GTCTTTATCG TCGACAACTT CGATGCTTTC GCCAAGATCC AAGACAACCT GATCACGTTC 
ATCTGTGAAA CGGCAACGTC CACATGCCCT TTGATTTACC TCAATGGATT CACATCACCA 
GGCTTCAGGA TGCTGGAAGC TTTCAACATC ACTGACAAGA CCTACGCAGG CATGAACGGC 
GTCTCTCTGG AAACCGGCTC CTTCAACAGC TTCATCGCAT ACAGACTCCA TAAGGACGCC 
TTCATTTCCC AGCCAACAAA GGAAATCCAT CCAGATGGTC TTCCTCCATC CTACACCCTC 
ATCCTCCTCT TCCGTCTGCT CCCGGACACC GGCAAGGATG CGTTTGATTT ATGGCAGATC 
TCAGATAAGA ACAATAACCC TGAGGTCGGG CTCACCATTG ACCCCTCCAG CAGAGTGATC 
TCCTTCTACA ATAAAGACAC TAGAGGGGAA ATTCAGAAAG TCTCCTTTGA TAACGACCAC 
GTGAAGAAGA TTTTCCACGG CAGTTTCCAC AAGCTTCACA TTTCTATTTC GCCGAAAAAT 
GTCAAACTCA ACATCGACTG CCAAGAGGTG GCTGAGAAAC AGATCAAAGC AGCCAACAAC 
GTCTCCACAG ATGGCTACGA AGTCCTTGGA AAAATGACAA AGTCTGGCGG TTCAAAGAAG 
CAGTCGGCCA CATTCCAGCT CCAGATGTTT GATATTGTCT GCAGTTTGGG CTGGACAAGT 
CGAGACAAAT GTTGTGATAT TCCAGCAATG CGAGATGAAG CCAAGTGTCC TGCTCTTCCT 
CACTCCTGCA CATGTGCTCA GGACAGTGTT GGTCCTCCAG GCCCAGCGGG TCCTCAAGGC 
AGTCCTGGTA CAAAAGGCCC ACGAGGCGAG AGAGGAGAGT CTGGTCCTAC GGGTCCAATG 
GGTCCACGTG GAGAGCAGGG TCTGCCAGGG CAGATGGGGC CACCGGGTCC ACAGGGCCCC 
AATGGACTAT CCCTTCCCGG AGAACCAGGG CGACCGGGAC CTAAGGGAGA CCCCGGTGAC 
TCAGGTTTAC CTGGACAGAG AGGCCCGGTT GGCGCTGCAG GTCCACTCGG CCCTGTTGGA 
CCCGCTGGAG CAAGAGGTCC ACCAGGAAGT GAAGGCCCAT CTGGACCAAG AGGTCCATCG 
GGACCAATGG GACCACCGGG AGCTCCCGGA ATGCCAGGAA TCACCGGAAA ACCAGGAAAA 
CCCGGAGACA CTGGCTTTCC TGGACCTGTT GGCATGAAAG GAGAGAAGGG TGAAAGAGGA 
GACACTGCGT CACAGAATAT GATGCGCTCT GTAGCGAGAC AAGTCTGCGA ACAGCTAGTC 
AGCAGACAAA TGAGCAGAAT TGATATGATG ATGAACCAGA TCCCCAGCAA CTACAGGAGT 
AACAGCCCCG GCCCCGCTGG ACCTCCAGGC CCACCTGGAA ACCAGGGACC CCATGGAGAG 
CCTGGTCAGC CCGGACCCAA TGGCTTCCCA GGAAATCCAG GTTTACCAGG CTCTCCTGGA 
GAAAGAGGAG TGCCTGGAGA GAAGGGTGAA CGAGGCAGCC CCGGCATTGG CACTCGAGGA 
CAGAGAGGAT TACCTGGACC TCCTGGGCCA CCAGGGCAGT CTCAGACCGG TCCCCCTGGT 
CCCTCAGGCT CACCCGGGCC CCGAGGACCA CCAGGACAAC ATGGTTCTTC TGGAGTCACA 
GGACCACCAG GACCTCCAGG ATACTGTGAC TCCTCTCAGT GCGGCATGCC TTACAACGGA 
CAAGGCTACC CAGGCCCCTA CCAGCCCGGG TCTGAAACCT ACGTAGTGCC CATACCAGCC 
GCGCCATCGG AAGATATCGA GACACAGTCG CCAGGATTGC GCTACCAGCG CGGAAAAAGG 
TCGCTATCAA GAAAGGAAGC CGAAAGAATC AAAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL12A1A ( XM_009293296.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009291571.1 Danio rerio collagen, type XII, alpha 1a (col12a1a), transcript variant X1, mRNA.

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CloneID	ODa48772
Clone ID Related Accession (Same CDS sequence)	XM_009293296.2 , XM_009293296.3
Accession Version	XM_009293296.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	collagen alpha-1(XII) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293296.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATCA GGTTGTCTTT GGCCGCTGCT GCGCTCTTGA CTCTAGTACT CAGTTCAGTA 
GAGGCTCAAG TGGAGCCTCC GTCAGACTTA AAATTTAAAA TCCTGAATGA AAACACAGTG 
CAAATGTCAT GGAGGAAGCC CTCGTCTCAG ATACAGGGCT ACAGAATACA GGTCACGTCA 
GATACAGATG ATTCTAAAGA ATTGAGTCTT CCTGCCTCGT CCACAAGCAC ATCAATTACA 
GATCTAACTC CAGATGTAGA CTATTCAGTG TCAATCAATT CATATGATGG TGCTGAAGAA 
AGTATCCCTA TCCTTGGACA AATCACAATT CAATCCAGCA ACACAAGTGG ACCAAGGAGG 
CCACAGTCAG AGGCTGTCAA GTGTTCAGTG AGTGCCGTTG CGGATCTGGT TTTCCTGGTG 
GACGGCTCTT GGAGTGTGGG AAGAGAAAAC TTTAGATTTA TCCGGAGTTT CATTGGGGCC 
ATGGCCGGGG CCTTCGACAT TGAAGAGGAT AAGACCAGAG TGGCTGTGGT TCAGTACAGC 
TCGGACACCA GGACAGAGTT CAGTCTAAAC ACACATTTCA GACGACCCGA TGTCCTGCGA 
GCCATTAAAA ATCTGCCGTA CAAGGGTGGA AATACCATGA CGGGTGATGC AATGGACTAT 
CTGGTGAAAA ACACGTTCAC TCAGGCGGCT GGAGCCAGGA AAGGCTTCCC AAAAGTGGCC 
ATGATCATCA CAGATGGAAA ATCTCAAGAC CCCGTAGAGG AGTACGCCGA ACGGTTGAGG 
AACATCGGAG TGGAGATCTT TGTGCTGGGT ATCAAAGGAG CAGATGAGGA CGAGCTGAAG 
GAGATCGCCT CCAGACCCCA TAGCAAGCAT GTCTATAACG TCCCCAACTT CGACATGATC 
AATCAGGTCC AGAAGGCGCT CATCACTGAA GTTTGCTCTG GCGTGGAGGA GCAGCTAAAT 
TCCCTTGTAA GCGGGGAGGA AGTTGTCGAA CCAGCTTCCA ACCTCAGAGT GACAGATATC 
TCCTCCAAAT CCATGAGAAT TGCGTGGGAT TCATCTCCTG GTGATATCAC AGGCTATAAG 
CTGCAGGTTT TTCCCACTAT TGCGAGCGCC AAAAAGCAGG AGCTCTACAC TGGGCCCACG 
ATGACCTTGG CCAATGTCAG AGACCTGACG CCTGAGATGG AGTATGAGAT CAGTCTGTTT 
GCTCTCAAAG GTCTGACTCC TAGTGTGCCG CAAGTTGTCA CTGAGAAAAC TCAGCCAGTG 
AAAGTATTTA CAGAGTGTTC CCTAGAGGTG GATGTCCAAG CTGACATTGT CCTCCTGGTG 
GATGGGTCAT ATAGTATAGG ACTGCCTAAC TTTGCGAAGG TTAGAGCTTT CCTCGAAGTA 
CTTGTGAATT CTTTTGACAT CGGACCTAAC AAAGTGCAGA TCAGCTTGGT TCAGTACAGC 
AGGGATCCTC ACACTGAGTT CGCCTTGAAT AAATTCGATG ACAATGCAGC CATGGTCAAG 
GCGGTCCGCA CCTTCCCCTA CCGAGGTGGA TCCACCAACA CCGGCAAGGC CATGACATAT 
GTGAGGGAAA AGATCTTTGT TTCGGGCCGT GGCGCTCGTG ATAACGTCCC CCGAGTCATG 
GTGCTTATCA CAGACGGCAA GTCCTCAGAT TCTTTCAAAG ACCCCGCCAA CAAACTGAGG 
GATACAGATG TGGAGATTTT TGCAGTGGGA GTAAAAGATG CCGTACGATC TGAGCTGGAG 
GCTATCGCCA ATGTTCCTGC TGATAACCAT GTGTTTGAGG TGGAGGACTT TGATGCTTTC 
GAGCGGATCT CCAAGGAGCT GACCGCGTCC ATCTGCCTCA GAATTGAGCA GGAGCTCCAT 
AACATCAGGA AAAGAAGTTT GCTTCCCCCA AAGTCACTGA GCTTCTCTGA GGTGACCTCC 
AGGAGTTTCA GGACCTCATG GGTTTCTGAT GGAGAGGATA TCCTGTCTTT TCTGGTGCGC 
TTCAGGCCAG CGGCGGACCT AACTGGAGAC TACATCTCTC TGGTGGTTCC CCATGACATC 
ACCAGTACAA TTCTGCCCAA TCTCACGCCA CTCACCACCT ACGAAGTTAA CGTCATTGCT 
CAGTATGATA AAGGAGACAG CTTTCCTTTG ACGGGCGAGG AGACCACTTT AGAAGAGTTG 
GGTTCTGTTC GTAACCTGAT GGTGAGCGAG GAGGCTGTGG ACCGCTTTCG GGTGTCGTGG 
AGAGCTGCTC CAGGATTAGT GCTGAGGTAC AGACTGCTCT ACGAGCCTGT GAGCGGAGGG 
GATAAACTGG AAGCAGAGAC TGATGGGAAT CAGGTCACCA CTGTGCTCAA TGAACTACTA 
CCGGTCACTA CTTACCGGGT CACCGTTTAC CCTGAGTACG CAAGTGGAAG GGGTCCCTCC 
ATGGACACTG AAGGCACCAC CAAAGAAGTT CGAGGTTCTC CACGCGACTT AAGGGTCTTC 
AATGAGACGG TGTCTAGCAT GAGGGTCTCG TGGCGGGCTG CCCCAGGCAA CGTGCTTCAA 
TATCAGGTGG CCTACAAACC TGACGGTGGG GAGAGGAAAG AAATAAGTGT TAAAGGAGAG 
ACAACAACTG CTGTGCTGAA AAATCTGCTG CCGGACACGG AGTATGAGCT GTTTGTTAGC 
GCACGTTACG TCTCTGGACT TGGCGCACCA CTTATGGGAA CAGGCACCAC GCTGGAAGAA 
CTTGGTTCAC CTTCGAATCT GGTAACTTCA GACGTCACTG AGAACAGTTT TGTAGCATCC 
TGGACCGCCG CCCCTGGAAA CGTCCGTCTT TATCGGGTCA CCTGGAAATC CCTCTTTTCA 
GAGGAAGCTG GCGAGAAGAC TGTCCCCGGG GACGTAACCA CCACTGTTCT GGACGGGCTG 
ACACCAGAGA CCCGCTACAA GGTTTCTGTC TACGCTGTCT ACAGCCGTGC GGAGGGGGCG 
CCACTGCACG GAGAGGAGAC CACAGATGTG TCGGCCGCTA CCAGAGCTCT TACGGTGTCA 
GAAGAAACAG AGAGGACCAT GAAGGTGACA TGGATACCAG CACCTGGCAA TGTTGTCAAT 
TACAGGGTAT CATACAAACC TCAGGGTGAT TTACGCCAAA TGGCCTCCAA AGCACCAGGA 
GGCACCAATT CTATAGTCCT GAGACGCCTG ATCCCCCAGA CCACTTACGA TATCACAGTG 
GTTCCCGTCT ACAAGTTTGG GGATGGAAGA CAAAGGCAAG GAGAAGGAAC TACATTGTCT 
CAATATAAAG CTCCCAGAAA CTTGCAAACT TCGGAGCCCA CTAAAACCAG TTTCAGGGTG 
ACCTGGGATC ATGCTCCTGG AGATGTAAAA GGCTACAAGG TCACTTTCCA TCCCACCGGA 
AAGGAAGTGG ACCTGGGAGA GTTACTCGTA GGCCCCTATG ACAACACTGT GGTTCTGGAG 
GAACTAAGGG CCGGCACTAA GTATACGGTA AATGTGTTTG GTATGTTTGA TGGAGGAGAG 
AGTATGCCAT TGGCTGGAGA GGAGAAGACC ACCCTCTCTG ACGCAGCTTC TGAAAACCTC 
CCTTATACAC CTTCAGATGC CCAGTGTAAA ACCACGGCCC AAGCGGATAT CGTCCTGCTT 
GTTGATGGAT CTTGGAGCAT TGGTCGCCTG AACTTCAAGA CCATACGCAA TTTCATTGCT 
CGCATGGTGG GAGTCTTCGA CATCGGCCCC GATCGTGTCC AGATTGGTCT GGCACAGTAC 
AGCGGAGACC CGAAGACTGA GTGGCATCTC AATGCTCACA GAACCAGGAC TCAGCTGCTG 
GATGCGGTGG CCAATCTACC CTACAAAGGA GGAAACACTC TCACCGGCCT TGCTTTGAAT 
TACATCCTTC AGAATAACTT CAAGCCTAAC GTTGGCATGC GTCCAAACTC CAGAAAGATC 
GGCGTCCTGG TCACTGATGG CAAGTCCCAA GACGACATTG TGGCGAACTC ACAGAACCTG 
CGGGACCAAG GGATTGAACT GTACGCCATT GGTGTAAAGA ACGCAGATGA GAATGAGTTG 
AGAACCATTG CTTCCGATCC CGATGACATT CACATGTACA ACGTGGCTGA TTTCTCCTTC 
CTGCTGGACA TTGTGGATGA CCTCACAAAC AACCTGTGCA ACAGCGTCAA AGGTCCAGGA 
GGAGGACCAG AGCCACCCAC TGATCTGACC ACCTCAGAGG TCACACATTA CTCTTTCCGT 
GCCACTTGGG TGGCTCCTGA CGGGCCTGTG GAGAAGTACA GAGTGGAGTA TGTGAGCACC 
GCTGGAGGAG AACCACAGCA ACAGATGTTT GTGGACGGGA CGGTGACCAC TGTGGTTTTG 
GAAGGTCTCA CTCCGCTGAC CGAGTACATA GTAAAGGTTT ACTCAGTGGT GGGAGAGGAC 
AGCAGCGACC CACTCAGAGG CATAGAGACC ACATTGCCTC TCAGTGCTGT GAGGAGCATG 
AACATCTACA AAGAGCGCTC CACCACCATG AATGTGAGAT GGGACGCAGC CACAGGGGCC 
ACAGGCTACA TGCTTCTCTA CAAGGCCCTT AACGCCACTG AACCCTCTGT GGAGCAGGAG 
ATGCGAGTGA AAGGAATGGT CACTGACGTC CAGCTGAAGA AACTTCTCCC CAACACGGCC 
TACAGTGTTT CGCTCTTCGC CCTGCACGGC GATTACGCCA GTGAACCTCT GATTGATCAA 
GGGGTGACCC TGCCTCTTCC TCCATCCGGT GAGTTGAGGA TTACCGATGT GACCCACAGC 
ACCATGCAGC TGGACTGGGA CGCTGCGCCC GGCGCCGTCC GAAAGTACAT CATCACTTAC 
AAGCCAGAGG AGGGAGAGGC GAAGGAGCTT GAAGTTGACG GAGATGTAAC ATCTAGAGGC 
CTGGATAATC TCATCTCCCA GACTGAATAT GATGTGGCTG TGACTCCGGT TTATGATTCG 
GGCACTGGAA ACCCCATGCT TAACCAGGCC ATTACAGATG TGGTTCCTGC CCCGCAGAAC 
TTGAGGTTCT CGATGGTGAC CCAGACCAGT TTTAGGGTTC ACTGGGAGCA CGGCGCTCCT 
GATGTCTCCC TCTACCGTAT TGGCTGGAAA AAGAAAGGAA CGACCGAGTT TCAAAATGAT 
ATCCTGAGTA GTGAAGACAC TACATATGAT CTGGTGGACC TGGAGCCAGA CACACCTTAT 
GATGTTTCGG TCACTGCCAT TTACCCTGAT GAGTCTGAGA GCGAGGATCT GCTGGGCACT 
GAACGCACTT TGGCCAAGCT TGCTACACCA CCTCCCAGTG GTCCTCCTCA AAACCTCCAG 
GTGTTCGACG AGACCACCAC CTCCCTCACG ACTAAGTGGG ACCACGCGCC TGGACGAGTT 
CAGAACTACA AAATCACCTA CGTACCGCTT ACTGGAGGAC GATCACAGTC GACGCAGGTC 
GGAGGAAAGA AAAACACAGT GATCCTGCAG AAACTGACTC CAGACACTCC TTACTCCATC 
ACTGTTGCTG CGGTCTACGC CAGCGGAGAG GCCAGAGACA TTTCTGGCCA AGGAAAGACA 
TTGCCGCTGG GCAGTGTCAG GAATCTTCAG GTCACTGACC CCACAGTTAG CACTCTGAAT 
GTGCGCTGGG AGCCGGCGGA GGGAAGCGTT CGCCAGTACA GGATCTACTA CCAGCCCGTC 
GCAGGGGGCG CAGAGGACAT GGTCCAGGTT CCTGGAAGCA CCACCAGCAC CGTTCTGAAG 
AACCTGCAAT CTGACACTCA GTACAGAGTG ACGGTGGTGC CAGTCTACGC TCCTGGAGAG 
GGCAAGCGCA TGTCGGAGAC CGGAAAAACA CTGGTTCGGA CCCCTGCCAA GAACATCCAG 
GTTTACAACC CCACCACAAA CACCCTGAAC GTGCGCTGGG AGCCGGCCTC AGGACAGGTG 
CAGCAGTACC GCGTGGCTTA CGCCCCTCTC TCCGGGATCC GACCCTCTCA GTCGGTGCTG 
GTTCCAGGAA ACACCCACAC GGCTCTGCTG CAGCAGCTGG AATCAGACAC TGACTACTCT 
GTGAGTGTGG TGGCTCTGTA TGCTGATGGA GAGGGCTCGT CTGTGTCTGA TAATGGCAAA 
ACACTGCCCC GCAGTGGCCC CAGGAACCTG CGGGTTTATG ATCCCACCAC CAGCACGCTG 
TCGGTGGGCT GGGAACATGC AGAAGGGCCT GTGATTCAGT ACCGCATTGG CTACGCACCC 
ACCACTGGAG ATCCCATTGA AGAATTTACA ACTGTCCCAG GCAACCGTAA CTCGGTCATC 
CTGCAAAACC TGCAGCCAGA CACGCCGTAC AACATCAACG TAGAAGCCAT TTATCAAGAT 
GGCCCTGGAG GACGACTCAG TGGAGATGGA AAAACATTGG ATCTCCTTGG GCCTCGTAAC 
CTGCGTGTTT CAGACGAGTG GTACACACGT TTCCGAGTGT CCTGGGATCC TGCAGCCGCA 
CGGGTGAATG GATATAAACT GGTCTATACA CCAGTGGGCA CAACCGACAC CATGGAAGCC 
TTCGTTGGAG ACGTGACTTC ACACCACCTG AACAACCTCC AGCCTGGCAC CACATACGAC 
CTGAAGGTTT ACGCTCAGTA TGATGCGGGC TTGAGCGGCG CTCTGGTCGG ACAGGGAACC 
ACCCTGTATC TCAACGTGAC CGACCTCACT TCCTACAACG TGGGCTACGA CTCTTTCTGC 
ATCAGATGGA CTCCTCATCG AGCTGCCACC TCCTACAGAC TCAAAGTCAA CCCTTTCAAC 
CCATCTCACC GTGGAGCTCA GGAGGTCACG ATCACTGCGG CTGAGAGCCA GTACTGTTTT 
GACGGCCTGA CGCCAGACGC GCTATACAAC GCCACCGTCT ACACGCAGAC CCCCAACCGA 
GAGGGGCCCG GAGTCAGTGT CAAAGAGCGC ACACTGGTGA AGCCCACCGT CGCCCCAACA 
CTACCTCCAA CTCCTCCACC TCCAGCCACC ATTCCTTCTG CACTCGAGGT GTGCAAAGGT 
GCCAAGGCTG ACCTGGTGTT CCTGATCGAT GGCTCGTGGA GTATCGGAGA TGACAGCTTC 
GCTAAAGTCC GGCAGTTTGT GTTCAGCATG ATTGGGGCGT TTGATGTGGT CAGCCATGAG 
GGCATGCAGG TGTCTTTCGT GCAGTACAGC GACGACGCCA AAACTGAGTT CAAGCTCAAC 
ACCTACCACG ACAAGGGAAT GGTGCTGTCT GCTCTTCAGA TGGTCAATTA TAGAGGAGGA 
AACACCAAAA CAGGTGCTGC ACTGAAGCAT GTTGGAGAAA AAGTGTTCAC CTCGGACAAT 
GGCATGAGGA GAACCGTTCC CAAGGTACTG GTTGTGGTGA CCGATGGACG TTCTCAAGAT 
GACGTGAAGA AGAGCGCTGC TAAACTACAG CATGCAGGTT ACAGTGTGTT TGTGGTGGGT 
GTCGCTGACA TTGACGTTAA TGAACTGAGG AACATTGGAA GCAAGCCCAG TGACAGACAC 
GTCTTTATCG TCGACAACTT CGATGCTTTC GCCAAGATCC AAGACAACCT GATCACGTTC 
ATCTGTGAAA CGGCAACGTC CACATGCCCT TTGATTTACC TCAATGGATT CACATCACCA 
GGCTTCAGGA TGCTGGAAGC TTTCAACATC ACTGACAAGA CCTACGCAGG CATGAACGGC 
GTCTCTCTGG AAACCGGCTC CTTCAACAGC TTCATCGCAT ACAGACTCCA TAAGGACGCC 
TTCATTTCCC AGCCAACAAA GGAAATCCAT CCAGATGGTC TTCCTCCATC CTACACCCTC 
ATCCTCCTCT TCCGTCTGCT CCCGGACACC GGCAAGGATG CGTTTGATTT ATGGCAGATC 
TCAGATAAGA ACAATAACCC TGAGGTCGGG CTCACCATTG ACCCCTCCAG CAGAGTGATC 
TCCTTCTACA ATAAAGACAC TAGAGGGGAA ATTCAGAAAG TCTCCTTTGA TAACGACCAC 
GTGAAGAAGA TTTTCCACGG CAGTTTCCAC AAGCTTCACA TTTCTATTTC GCCGAAAAAT 
GTCAAACTCA ACATCGACTG CCAAGAGGTG GCTGAGAAAC AGATCAAAGC AGCCAACAAC 
GTCTCCACAG ATGGCTACGA AGTCCTTGGA AAAATGACAA AGTCTGGCGG TTCAAAGAAG 
CAGTCGGCCA CATTCCAGCT CCAGATGTTT GATATTGTCT GCAGTTTGGG CTGGACAAGT 
CGAGACAAAT GTTGTGATAT TCCAGCAATG CGAGATGAAG CCAAGTGTCC TGCTCTTCCT 
CACTCCTGCA CATGTGCTCA GGACAGTGTT GGTCCTCCAG GCCCAGCGGG TCCTCAAGGC 
AGTCCTGGTA CAAAAGGCCC ACGAGGCGAG AGAGGAGAGT CTGGTCCTAC GGGTCCAATG 
GGTCCACGTG GAGAGCAGGG TCTGCCAGGG CAGATGGGGC CACCGGGTCC ACAGGGCCCC 
AATGGACTAT CCCTTCCCGG AGAACCAGGG CGACCGGGAC CTAAGGGAGA CCCCGGTGAC 
TCAGGTTTAC CTGGACAGAG AGGCCCGGTT GGCGCTGCAG GTCCACTCGG CCCTGTTGGA 
CCCGCTGGAG CAAGAGGTCC ACCAGGAAGT GAAGGCCCAT CTGGACCAAG AGGTCCATCG 
GGACCAATGG GACCACCGGG AGCTCCCGGA ATGCCAGGAA TCACCGGAAA ACCAGGAAAA 
CCCGGAGACA CTGGCTTTCC TGGACCTGTT GGCATGAAAG GAGAGAAGGG TGAAAGAGGA 
GACACTGCGT CACAGAATAT GATGCGCTCT GTAGCGAGAC AAGTCTGCGA ACAGCTAGTC 
AGCAGACAAA TGAGCAGAAT TGATATGATG ATGAACCAGA TCCCCAGCAA CTACAGGAGT 
AACAGCCCCG GCCCCGCTGG ACCTCCAGGC CCACCTGGAA ACCAGGGACC CCATGGAGAG 
CCTGGTCAGC CCGGACCCAA TGGCTTCCCA GGAAATCCAG GTTTACCAGG CTCTCCTGGA 
GAAAGAGGAG TGCCTGGAGA GAAGGGTGAA CGAGGCAGCC CCGGCATTGG CACTCGAGGA 
CAGAGAGGAT TACCTGGACC TCCTGGGCCA CCAGGGCAGT CTCAGACCGG TCCCCCTGGT 
CCCTCAGGCT CACCCGGGCC CCGAGGACCA CCAGGACAAC ATGGTTCTTC TGGAGTCACA 
GGACCACCAG GACCTCCAGG ATACTGTGAC TCCTCTCAGT GCGGCATGCC TTACAACGGA 
CAAGGCTACC CAGGCCCCTA CCAGCCCGGG TCTGAAACCT ACGTAGTGCC CATACCAGCC 
GCGCCATCGG AAGATATCGA GACACAGTCG CCAGGATTGC GCTACCAGCG CGGAAAAAGG 
TCGCTATCAA GAAAGGAAGC CGAAAGAATC AAAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291571.1
CDS	180..9518
Translation	MKIRLSLAAAALLTLVLSSVEAQVEPPSDLKFKILNENTVQMSWRKPSSQIQGYRIQVTSDTDDSKELSLPASSTSTSITDLTPDVDYSVSINSYDGAEESIPILGQITIQSSNTSGPRRPQSEAVKCSVSAVADLVFLVDGSWSVGRENFRFIRSFIGAMAGAFDIEEDKTRVAVVQYSSDTRTEFSLNTHFRRPDVLRAIKNLPYKGGNTMTGDAMDYLVKNTFTQAAGARKGFPKVAMIITDGKSQDPVEEYAERLRNIGVEIFVLGIKGADEDELKEIASRPHSKHVYNVPNFDMINQVQKALITEVCSGVEEQLNSLVSGEEVVEPASNLRVTDISSKSMRIAWDSSPGDITGYKLQVFPTIASAKKQELYTGPTMTLANVRDLTPEMEYEISLFALKGLTPSVPQVVTEKTQPVKVFTECSLEVDVQADIVLLVDGSYSIGLPNFAKVRAFLEVLVNSFDIGPNKVQISLVQYSRDPHTEFALNKFDDNAAMVKAVRTFPYRGGSTNTGKAMTYVREKIFVSGRGARDNVPRVMVLITDGKSSDSFKDPANKLRDTDVEIFAVGVKDAVRSELEAIANVPADNHVFEVEDFDAFERISKELTASICLRIEQELHNIRKRSLLPPKSLSFSEVTSRSFRTSWVSDGEDILSFLVRFRPAADLTGDYISLVVPHDITSTILPNLTPLTTYEVNVIAQYDKGDSFPLTGEETTLEELGSVRNLMVSEEAVDRFRVSWRAAPGLVLRYRLLYEPVSGGDKLEAETDGNQVTTVLNELLPVTTYRVTVYPEYASGRGPSMDTEGTTKEVRGSPRDLRVFNETVSSMRVSWRAAPGNVLQYQVAYKPDGGERKEISVKGETTTAVLKNLLPDTEYELFVSARYVSGLGAPLMGTGTTLEELGSPSNLVTSDVTENSFVASWTAAPGNVRLYRVTWKSLFSEEAGEKTVPGDVTTTVLDGLTPETRYKVSVYAVYSRAEGAPLHGEETTDVSAATRALTVSEETERTMKVTWIPAPGNVVNYRVSYKPQGDLRQMASKAPGGTNSIVLRRLIPQTTYDITVVPVYKFGDGRQRQGEGTTLSQYKAPRNLQTSEPTKTSFRVTWDHAPGDVKGYKVTFHPTGKEVDLGELLVGPYDNTVVLEELRAGTKYTVNVFGMFDGGESMPLAGEEKTTLSDAASENLPYTPSDAQCKTTAQADIVLLVDGSWSIGRLNFKTIRNFIARMVGVFDIGPDRVQIGLAQYSGDPKTEWHLNAHRTRTQLLDAVANLPYKGGNTLTGLALNYILQNNFKPNVGMRPNSRKIGVLVTDGKSQDDIVANSQNLRDQGIELYAIGVKNADENELRTIASDPDDIHMYNVADFSFLLDIVDDLTNNLCNSVKGPGGGPEPPTDLTTSEVTHYSFRATWVAPDGPVEKYRVEYVSTAGGEPQQQMFVDGTVTTVVLEGLTPLTEYIVKVYSVVGEDSSDPLRGIETTLPLSAVRSMNIYKERSTTMNVRWDAATGATGYMLLYKALNATEPSVEQEMRVKGMVTDVQLKKLLPNTAYSVSLFALHGDYASEPLIDQGVTLPLPPSGELRITDVTHSTMQLDWDAAPGAVRKYIITYKPEEGEAKELEVDGDVTSRGLDNLISQTEYDVAVTPVYDSGTGNPMLNQAITDVVPAPQNLRFSMVTQTSFRVHWEHGAPDVSLYRIGWKKKGTTEFQNDILSSEDTTYDLVDLEPDTPYDVSVTAIYPDESESEDLLGTERTLAKLATPPPSGPPQNLQVFDETTTSLTTKWDHAPGRVQNYKITYVPLTGGRSQSTQVGGKKNTVILQKLTPDTPYSITVAAVYASGEARDISGQGKTLPLGSVRNLQVTDPTVSTLNVRWEPAEGSVRQYRIYYQPVAGGAEDMVQVPGSTTSTVLKNLQSDTQYRVTVVPVYAPGEGKRMSETGKTLVRTPAKNIQVYNPTTNTLNVRWEPASGQVQQYRVAYAPLSGIRPSQSVLVPGNTHTALLQQLESDTDYSVSVVALYADGEGSSVSDNGKTLPRSGPRNLRVYDPTTSTLSVGWEHAEGPVIQYRIGYAPTTGDPIEEFTTVPGNRNSVILQNLQPDTPYNINVEAIYQDGPGGRLSGDGKTLDLLGPRNLRVSDEWYTRFRVSWDPAAARVNGYKLVYTPVGTTDTMEAFVGDVTSHHLNNLQPGTTYDLKVYAQYDAGLSGALVGQGTTLYLNVTDLTSYNVGYDSFCIRWTPHRAATSYRLKVNPFNPSHRGAQEVTITAAESQYCFDGLTPDALYNATVYTQTPNREGPGVSVKERTLVKPTVAPTLPPTPPPPATIPSALEVCKGAKADLVFLIDGSWSIGDDSFAKVRQFVFSMIGAFDVVSHEGMQVSFVQYSDDAKTEFKLNTYHDKGMVLSALQMVNYRGGNTKTGAALKHVGEKVFTSDNGMRRTVPKVLVVVTDGRSQDDVKKSAAKLQHAGYSVFVVGVADIDVNELRNIGSKPSDRHVFIVDNFDAFAKIQDNLITFICETATSTCPLIYLNGFTSPGFRMLEAFNITDKTYAGMNGVSLETGSFNSFIAYRLHKDAFISQPTKEIHPDGLPPSYTLILLFRLLPDTGKDAFDLWQISDKNNNPEVGLTIDPSSRVISFYNKDTRGEIQKVSFDNDHVKKIFHGSFHKLHISISPKNVKLNIDCQEVAEKQIKAANNVSTDGYEVLGKMTKSGGSKKQSATFQLQMFDIVCSLGWTSRDKCCDIPAMRDEAKCPALPHSCTCAQDSVGPPGPAGPQGSPGTKGPRGERGESGPTGPMGPRGEQGLPGQMGPPGPQGPNGLSLPGEPGRPGPKGDPGDSGLPGQRGPVGAAGPLGPVGPAGARGPPGSEGPSGPRGPSGPMGPPGAPGMPGITGKPGKPGDTGFPGPVGMKGEKGERGDTASQNMMRSVARQVCEQLVSRQMSRIDMMMNQIPSNYRSNSPGPAGPPGPPGNQGPHGEPGQPGPNGFPGNPGLPGSPGERGVPGEKGERGSPGIGTRGQRGLPGPPGPPGQSQTGPPGPSGSPGPRGPPGQHGSSGVTGPPGPPGYCDSSQCGMPYNGQGYPGPYQPGSETYVVPIPAAPSEDIETQSPGLRYQRGKRSLSRKEAERIKT

Target ORF information:

RefSeq Version	XM_009293296.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type XII, alpha 1a (col12a1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293296.3

ORF Insert Sequence:

ATGAAGATCA GGTTGTCTTT GGCCGCTGCT GCGCTCTTGA CTCTAGTACT CAGTTCAGTA 
GAGGCTCAAG TGGAGCCTCC GTCAGACTTA AAATTTAAAA TCCTGAATGA AAACACAGTG 
CAAATGTCAT GGAGGAAGCC CTCGTCTCAG ATACAGGGCT ACAGAATACA GGTCACGTCA 
GATACAGATG ATTCTAAAGA ATTGAGTCTT CCTGCCTCGT CCACAAGCAC ATCAATTACA 
GATCTAACTC CAGATGTAGA CTATTCAGTG TCAATCAATT CATATGATGG TGCTGAAGAA 
AGTATCCCTA TCCTTGGACA AATCACAATT CAATCCAGCA ACACAAGTGG ACCAAGGAGG 
CCACAGTCAG AGGCTGTCAA GTGTTCAGTG AGTGCCGTTG CGGATCTGGT TTTCCTGGTG 
GACGGCTCTT GGAGTGTGGG AAGAGAAAAC TTTAGATTTA TCCGGAGTTT CATTGGGGCC 
ATGGCCGGGG CCTTCGACAT TGAAGAGGAT AAGACCAGAG TGGCTGTGGT TCAGTACAGC 
TCGGACACCA GGACAGAGTT CAGTCTAAAC ACACATTTCA GACGACCCGA TGTCCTGCGA 
GCCATTAAAA ATCTGCCGTA CAAGGGTGGA AATACCATGA CGGGTGATGC AATGGACTAT 
CTGGTGAAAA ACACGTTCAC TCAGGCGGCT GGAGCCAGGA AAGGCTTCCC AAAAGTGGCC 
ATGATCATCA CAGATGGAAA ATCTCAAGAC CCCGTAGAGG AGTACGCCGA ACGGTTGAGG 
AACATCGGAG TGGAGATCTT TGTGCTGGGT ATCAAAGGAG CAGATGAGGA CGAGCTGAAG 
GAGATCGCCT CCAGACCCCA TAGCAAGCAT GTCTATAACG TCCCCAACTT CGACATGATC 
AATCAGGTCC AGAAGGCGCT CATCACTGAA GTTTGCTCTG GCGTGGAGGA GCAGCTAAAT 
TCCCTTGTAA GCGGGGAGGA AGTTGTCGAA CCAGCTTCCA ACCTCAGAGT GACAGATATC 
TCCTCCAAAT CCATGAGAAT TGCGTGGGAT TCATCTCCTG GTGATATCAC AGGCTATAAG 
CTGCAGGTTT TTCCCACTAT TGCGAGCGCC AAAAAGCAGG AGCTCTACAC TGGGCCCACG 
ATGACCTTGG CCAATGTCAG AGACCTGACG CCTGAGATGG AGTATGAGAT CAGTCTGTTT 
GCTCTCAAAG GTCTGACTCC TAGTGTGCCG CAAGTTGTCA CTGAGAAAAC TCAGCCAGTG 
AAAGTATTTA CAGAGTGTTC CCTAGAGGTG GATGTCCAAG CTGACATTGT CCTCCTGGTG 
GATGGGTCAT ATAGTATAGG ACTGCCTAAC TTTGCGAAGG TTAGAGCTTT CCTCGAAGTA 
CTTGTGAATT CTTTTGACAT CGGACCTAAC AAAGTGCAGA TCAGCTTGGT TCAGTACAGC 
AGGGATCCTC ACACTGAGTT CGCCTTGAAT AAATTCGATG ACAATGCAGC CATGGTCAAG 
GCGGTCCGCA CCTTCCCCTA CCGAGGTGGA TCCACCAACA CCGGCAAGGC CATGACATAT 
GTGAGGGAAA AGATCTTTGT TTCGGGCCGT GGCGCTCGTG ATAACGTCCC CCGAGTCATG 
GTGCTTATCA CAGACGGCAA GTCCTCAGAT TCTTTCAAAG ACCCCGCCAA CAAACTGAGG 
GATACAGATG TGGAGATTTT TGCAGTGGGA GTAAAAGATG CCGTACGATC TGAGCTGGAG 
GCTATCGCCA ATGTTCCTGC TGATAACCAT GTGTTTGAGG TGGAGGACTT TGATGCTTTC 
GAGCGGATCT CCAAGGAGCT GACCGCGTCC ATCTGCCTCA GAATTGAGCA GGAGCTCCAT 
AACATCAGGA AAAGAAGTTT GCTTCCCCCA AAGTCACTGA GCTTCTCTGA GGTGACCTCC 
AGGAGTTTCA GGACCTCATG GGTTTCTGAT GGAGAGGATA TCCTGTCTTT TCTGGTGCGC 
TTCAGGCCAG CGGCGGACCT AACTGGAGAC TACATCTCTC TGGTGGTTCC CCATGACATC 
ACCAGTACAA TTCTGCCCAA TCTCACGCCA CTCACCACCT ACGAAGTTAA CGTCATTGCT 
CAGTATGATA AAGGAGACAG CTTTCCTTTG ACGGGCGAGG AGACCACTTT AGAAGAGTTG 
GGTTCTGTTC GTAACCTGAT GGTGAGCGAG GAGGCTGTGG ACCGCTTTCG GGTGTCGTGG 
AGAGCTGCTC CAGGATTAGT GCTGAGGTAC AGACTGCTCT ACGAGCCTGT GAGCGGAGGG 
GATAAACTGG AAGCAGAGAC TGATGGGAAT CAGGTCACCA CTGTGCTCAA TGAACTACTA 
CCGGTCACTA CTTACCGGGT CACCGTTTAC CCTGAGTACG CAAGTGGAAG GGGTCCCTCC 
ATGGACACTG AAGGCACCAC CAAAGAAGTT CGAGGTTCTC CACGCGACTT AAGGGTCTTC 
AATGAGACGG TGTCTAGCAT GAGGGTCTCG TGGCGGGCTG CCCCAGGCAA CGTGCTTCAA 
TATCAGGTGG CCTACAAACC TGACGGTGGG GAGAGGAAAG AAATAAGTGT TAAAGGAGAG 
ACAACAACTG CTGTGCTGAA AAATCTGCTG CCGGACACGG AGTATGAGCT GTTTGTTAGC 
GCACGTTACG TCTCTGGACT TGGCGCACCA CTTATGGGAA CAGGCACCAC GCTGGAAGAA 
CTTGGTTCAC CTTCGAATCT GGTAACTTCA GACGTCACTG AGAACAGTTT TGTAGCATCC 
TGGACCGCCG CCCCTGGAAA CGTCCGTCTT TATCGGGTCA CCTGGAAATC CCTCTTTTCA 
GAGGAAGCTG GCGAGAAGAC TGTCCCCGGG GACGTAACCA CCACTGTTCT GGACGGGCTG 
ACACCAGAGA CCCGCTACAA GGTTTCTGTC TACGCTGTCT ACAGCCGTGC GGAGGGGGCG 
CCACTGCACG GAGAGGAGAC CACAGATGTG TCGGCCGCTA CCAGAGCTCT TACGGTGTCA 
GAAGAAACAG AGAGGACCAT GAAGGTGACA TGGATACCAG CACCTGGCAA TGTTGTCAAT 
TACAGGGTAT CATACAAACC TCAGGGTGAT TTACGCCAAA TGGCCTCCAA AGCACCAGGA 
GGCACCAATT CTATAGTCCT GAGACGCCTG ATCCCCCAGA CCACTTACGA TATCACAGTG 
GTTCCCGTCT ACAAGTTTGG GGATGGAAGA CAAAGGCAAG GAGAAGGAAC TACATTGTCT 
CAATATAAAG CTCCCAGAAA CTTGCAAACT TCGGAGCCCA CTAAAACCAG TTTCAGGGTG 
ACCTGGGATC ATGCTCCTGG AGATGTAAAA GGCTACAAGG TCACTTTCCA TCCCACCGGA 
AAGGAAGTGG ACCTGGGAGA GTTACTCGTA GGCCCCTATG ACAACACTGT GGTTCTGGAG 
GAACTAAGGG CCGGCACTAA GTATACGGTA AATGTGTTTG GTATGTTTGA TGGAGGAGAG 
AGTATGCCAT TGGCTGGAGA GGAGAAGACC ACCCTCTCTG ACGCAGCTTC TGAAAACCTC 
CCTTATACAC CTTCAGATGC CCAGTGTAAA ACCACGGCCC AAGCGGATAT CGTCCTGCTT 
GTTGATGGAT CTTGGAGCAT TGGTCGCCTG AACTTCAAGA CCATACGCAA TTTCATTGCT 
CGCATGGTGG GAGTCTTCGA CATCGGCCCC GATCGTGTCC AGATTGGTCT GGCACAGTAC 
AGCGGAGACC CGAAGACTGA GTGGCATCTC AATGCTCACA GAACCAGGAC TCAGCTGCTG 
GATGCGGTGG CCAATCTACC CTACAAAGGA GGAAACACTC TCACCGGCCT TGCTTTGAAT 
TACATCCTTC AGAATAACTT CAAGCCTAAC GTTGGCATGC GTCCAAACTC CAGAAAGATC 
GGCGTCCTGG TCACTGATGG CAAGTCCCAA GACGACATTG TGGCGAACTC ACAGAACCTG 
CGGGACCAAG GGATTGAACT GTACGCCATT GGTGTAAAGA ACGCAGATGA GAATGAGTTG 
AGAACCATTG CTTCCGATCC CGATGACATT CACATGTACA ACGTGGCTGA TTTCTCCTTC 
CTGCTGGACA TTGTGGATGA CCTCACAAAC AACCTGTGCA ACAGCGTCAA AGGTCCAGGA 
GGAGGACCAG AGCCACCCAC TGATCTGACC ACCTCAGAGG TCACACATTA CTCTTTCCGT 
GCCACTTGGG TGGCTCCTGA CGGGCCTGTG GAGAAGTACA GAGTGGAGTA TGTGAGCACC 
GCTGGAGGAG AACCACAGCA ACAGATGTTT GTGGACGGGA CGGTGACCAC TGTGGTTTTG 
GAAGGTCTCA CTCCGCTGAC CGAGTACATA GTAAAGGTTT ACTCAGTGGT GGGAGAGGAC 
AGCAGCGACC CACTCAGAGG CATAGAGACC ACATTGCCTC TCAGTGCTGT GAGGAGCATG 
AACATCTACA AAGAGCGCTC CACCACCATG AATGTGAGAT GGGACGCAGC CACAGGGGCC 
ACAGGCTACA TGCTTCTCTA CAAGGCCCTT AACGCCACTG AACCCTCTGT GGAGCAGGAG 
ATGCGAGTGA AAGGAATGGT CACTGACGTC CAGCTGAAGA AACTTCTCCC CAACACGGCC 
TACAGTGTTT CGCTCTTCGC CCTGCACGGC GATTACGCCA GTGAACCTCT GATTGATCAA 
GGGGTGACCC TGCCTCTTCC TCCATCCGGT GAGTTGAGGA TTACCGATGT GACCCACAGC 
ACCATGCAGC TGGACTGGGA CGCTGCGCCC GGCGCCGTCC GAAAGTACAT CATCACTTAC 
AAGCCAGAGG AGGGAGAGGC GAAGGAGCTT GAAGTTGACG GAGATGTAAC ATCTAGAGGC 
CTGGATAATC TCATCTCCCA GACTGAATAT GATGTGGCTG TGACTCCGGT TTATGATTCG 
GGCACTGGAA ACCCCATGCT TAACCAGGCC ATTACAGATG TGGTTCCTGC CCCGCAGAAC 
TTGAGGTTCT CGATGGTGAC CCAGACCAGT TTTAGGGTTC ACTGGGAGCA CGGCGCTCCT 
GATGTCTCCC TCTACCGTAT TGGCTGGAAA AAGAAAGGAA CGACCGAGTT TCAAAATGAT 
ATCCTGAGTA GTGAAGACAC TACATATGAT CTGGTGGACC TGGAGCCAGA CACACCTTAT 
GATGTTTCGG TCACTGCCAT TTACCCTGAT GAGTCTGAGA GCGAGGATCT GCTGGGCACT 
GAACGCACTT TGGCCAAGCT TGCTACACCA CCTCCCAGTG GTCCTCCTCA AAACCTCCAG 
GTGTTCGACG AGACCACCAC CTCCCTCACG ACTAAGTGGG ACCACGCGCC TGGACGAGTT 
CAGAACTACA AAATCACCTA CGTACCGCTT ACTGGAGGAC GATCACAGTC GACGCAGGTC 
GGAGGAAAGA AAAACACAGT GATCCTGCAG AAACTGACTC CAGACACTCC TTACTCCATC 
ACTGTTGCTG CGGTCTACGC CAGCGGAGAG GCCAGAGACA TTTCTGGCCA AGGAAAGACA 
TTGCCGCTGG GCAGTGTCAG GAATCTTCAG GTCACTGACC CCACAGTTAG CACTCTGAAT 
GTGCGCTGGG AGCCGGCGGA GGGAAGCGTT CGCCAGTACA GGATCTACTA CCAGCCCGTC 
GCAGGGGGCG CAGAGGACAT GGTCCAGGTT CCTGGAAGCA CCACCAGCAC CGTTCTGAAG 
AACCTGCAAT CTGACACTCA GTACAGAGTG ACGGTGGTGC CAGTCTACGC TCCTGGAGAG 
GGCAAGCGCA TGTCGGAGAC CGGAAAAACA CTGGTTCGGA CCCCTGCCAA GAACATCCAG 
GTTTACAACC CCACCACAAA CACCCTGAAC GTGCGCTGGG AGCCGGCCTC AGGACAGGTG 
CAGCAGTACC GCGTGGCTTA CGCCCCTCTC TCCGGGATCC GACCCTCTCA GTCGGTGCTG 
GTTCCAGGAA ACACCCACAC GGCTCTGCTG CAGCAGCTGG AATCAGACAC TGACTACTCT 
GTGAGTGTGG TGGCTCTGTA TGCTGATGGA GAGGGCTCGT CTGTGTCTGA TAATGGCAAA 
ACACTGCCCC GCAGTGGCCC CAGGAACCTG CGGGTTTATG ATCCCACCAC CAGCACGCTG 
TCGGTGGGCT GGGAACATGC AGAAGGGCCT GTGATTCAGT ACCGCATTGG CTACGCACCC 
ACCACTGGAG ATCCCATTGA AGAATTTACA ACTGTCCCAG GCAACCGTAA CTCGGTCATC 
CTGCAAAACC TGCAGCCAGA CACGCCGTAC AACATCAACG TAGAAGCCAT TTATCAAGAT 
GGCCCTGGAG GACGACTCAG TGGAGATGGA AAAACATTGG ATCTCCTTGG GCCTCGTAAC 
CTGCGTGTTT CAGACGAGTG GTACACACGT TTCCGAGTGT CCTGGGATCC TGCAGCCGCA 
CGGGTGAATG GATATAAACT GGTCTATACA CCAGTGGGCA CAACCGACAC CATGGAAGCC 
TTCGTTGGAG ACGTGACTTC ACACCACCTG AACAACCTCC AGCCTGGCAC CACATACGAC 
CTGAAGGTTT ACGCTCAGTA TGATGCGGGC TTGAGCGGCG CTCTGGTCGG ACAGGGAACC 
ACCCTGTATC TCAACGTGAC CGACCTCACT TCCTACAACG TGGGCTACGA CTCTTTCTGC 
ATCAGATGGA CTCCTCATCG AGCTGCCACC TCCTACAGAC TCAAAGTCAA CCCTTTCAAC 
CCATCTCACC GTGGAGCTCA GGAGGTCACG ATCACTGCGG CTGAGAGCCA GTACTGTTTT 
GACGGCCTGA CGCCAGACGC GCTATACAAC GCCACCGTCT ACACGCAGAC CCCCAACCGA 
GAGGGGCCCG GAGTCAGTGT CAAAGAGCGC ACACTGGTGA AGCCCACCGT CGCCCCAACA 
CTACCTCCAA CTCCTCCACC TCCAGCCACC ATTCCTTCTG CACTCGAGGT GTGCAAAGGT 
GCCAAGGCTG ACCTGGTGTT CCTGATCGAT GGCTCGTGGA GTATCGGAGA TGACAGCTTC 
GCTAAAGTCC GGCAGTTTGT GTTCAGCATG ATTGGGGCGT TTGATGTGGT CAGCCATGAG 
GGCATGCAGG TGTCTTTCGT GCAGTACAGC GACGACGCCA AAACTGAGTT CAAGCTCAAC 
ACCTACCACG ACAAGGGAAT GGTGCTGTCT GCTCTTCAGA TGGTCAATTA TAGAGGAGGA 
AACACCAAAA CAGGTGCTGC ACTGAAGCAT GTTGGAGAAA AAGTGTTCAC CTCGGACAAT 
GGCATGAGGA GAACCGTTCC CAAGGTACTG GTTGTGGTGA CCGATGGACG TTCTCAAGAT 
GACGTGAAGA AGAGCGCTGC TAAACTACAG CATGCAGGTT ACAGTGTGTT TGTGGTGGGT 
GTCGCTGACA TTGACGTTAA TGAACTGAGG AACATTGGAA GCAAGCCCAG TGACAGACAC 
GTCTTTATCG TCGACAACTT CGATGCTTTC GCCAAGATCC AAGACAACCT GATCACGTTC 
ATCTGTGAAA CGGCAACGTC CACATGCCCT TTGATTTACC TCAATGGATT CACATCACCA 
GGCTTCAGGA TGCTGGAAGC TTTCAACATC ACTGACAAGA CCTACGCAGG CATGAACGGC 
GTCTCTCTGG AAACCGGCTC CTTCAACAGC TTCATCGCAT ACAGACTCCA TAAGGACGCC 
TTCATTTCCC AGCCAACAAA GGAAATCCAT CCAGATGGTC TTCCTCCATC CTACACCCTC 
ATCCTCCTCT TCCGTCTGCT CCCGGACACC GGCAAGGATG CGTTTGATTT ATGGCAGATC 
TCAGATAAGA ACAATAACCC TGAGGTCGGG CTCACCATTG ACCCCTCCAG CAGAGTGATC 
TCCTTCTACA ATAAAGACAC TAGAGGGGAA ATTCAGAAAG TCTCCTTTGA TAACGACCAC 
GTGAAGAAGA TTTTCCACGG CAGTTTCCAC AAGCTTCACA TTTCTATTTC GCCGAAAAAT 
GTCAAACTCA ACATCGACTG CCAAGAGGTG GCTGAGAAAC AGATCAAAGC AGCCAACAAC 
GTCTCCACAG ATGGCTACGA AGTCCTTGGA AAAATGACAA AGTCTGGCGG TTCAAAGAAG 
CAGTCGGCCA CATTCCAGCT CCAGATGTTT GATATTGTCT GCAGTTTGGG CTGGACAAGT 
CGAGACAAAT GTTGTGATAT TCCAGCAATG CGAGATGAAG CCAAGTGTCC TGCTCTTCCT 
CACTCCTGCA CATGTGCTCA GGACAGTGTT GGTCCTCCAG GCCCAGCGGG TCCTCAAGGC 
AGTCCTGGTA CAAAAGGCCC ACGAGGCGAG AGAGGAGAGT CTGGTCCTAC GGGTCCAATG 
GGTCCACGTG GAGAGCAGGG TCTGCCAGGG CAGATGGGGC CACCGGGTCC ACAGGGCCCC 
AATGGACTAT CCCTTCCCGG AGAACCAGGG CGACCGGGAC CTAAGGGAGA CCCCGGTGAC 
TCAGGTTTAC CTGGACAGAG AGGCCCGGTT GGCGCTGCAG GTCCACTCGG CCCTGTTGGA 
CCCGCTGGAG CAAGAGGTCC ACCAGGAAGT GAAGGCCCAT CTGGACCAAG AGGTCCATCG 
GGACCAATGG GACCACCGGG AGCTCCCGGA ATGCCAGGAA TCACCGGAAA ACCAGGAAAA 
CCCGGAGACA CTGGCTTTCC TGGACCTGTT GGCATGAAAG GAGAGAAGGG TGAAAGAGGA 
GACACTGCGT CACAGAATAT GATGCGCTCT GTAGCGAGAC AAGTCTGCGA ACAGCTAGTC 
AGCAGACAAA TGAGCAGAAT TGATATGATG ATGAACCAGA TCCCCAGCAA CTACAGGAGT 
AACAGCCCCG GCCCCGCTGG ACCTCCAGGC CCACCTGGAA ACCAGGGACC CCATGGAGAG 
CCTGGTCAGC CCGGACCCAA TGGCTTCCCA GGAAATCCAG GTTTACCAGG CTCTCCTGGA 
GAAAGAGGAG TGCCTGGAGA GAAGGGTGAA CGAGGCAGCC CCGGCATTGG CACTCGAGGA 
CAGAGAGGAT TACCTGGACC TCCTGGGCCA CCAGGGCAGT CTCAGACCGG TCCCCCTGGT 
CCCTCAGGCT CACCCGGGCC CCGAGGACCA CCAGGACAAC ATGGTTCTTC TGGAGTCACA 
GGACCACCAG GACCTCCAGG ATACTGTGAC TCCTCTCAGT GCGGCATGCC TTACAACGGA 
CAAGGCTACC CAGGCCCCTA CCAGCCCGGG TCTGAAACCT ACGTAGTGCC CATACCAGCC 
GCGCCATCGG AAGATATCGA GACACAGTCG CCAGGATTGC GCTACCAGCG CGGAAAAAGG 
TCGCTATCAA GAAAGGAAGC CGAAAGAATC AAAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL12A1A ( XM_009293297.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009291572.1 Danio rerio collagen, type XII, alpha 1a (col12a1a), transcript variant X3, mRNA.

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CloneID	ODa48773
Clone ID Related Accession (Same CDS sequence)	XM_009293297.3 , XM_009293297.2
Accession Version	XM_009293297.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9063bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	collagen alpha-1(XII) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293297.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATCA GGTTGTCTTT GGCCGCTGCT GCGCTCTTGA CTCTAGTACT CAGTTCAGTA 
GAGGCTCAAG TGGAGCCTCC GTCAGACTTA AAATTTAAAA TCCTGAATGA AAACACAGTG 
CAAATGTCAT GGAGGAAGCC CTCGTCTCAG ATACAGGGCT ACAGAATACA GGTCACGTCA 
GATACAGATG ATTCTAAAGA ATTGAGTCTT CCTGCCTCGT CCACAAGCAC ATCAATTACA 
GATCTAACTC CAGATGTAGA CTATTCAGTG TCAATCAATT CATATGATGG TGCTGAAGAA 
AGTATCCCTA TCCTTGGACA AATCACAATT CAATCCAGCA ACACAAGTGG ACCAAGGAGG 
CCACAGTCAG AGGCTGTCAA GTGTTCAGTG AGTGCCGTTG CGGATCTGGT TTTCCTGGTG 
GACGGCTCTT GGAGTGTGGG AAGAGAAAAC TTTAGATTTA TCCGGAGTTT CATTGGGGCC 
ATGGCCGGGG CCTTCGACAT TGAAGAGGAT AAGACCAGAG TGGCTGTGGT TCAGTACAGC 
TCGGACACCA GGACAGAGTT CAGTCTAAAC ACACATTTCA GACGACCCGA TGTCCTGCGA 
GCCATTAAAA ATCTGCCGTA CAAGGGTGGA AATACCATGA CGGGTGATGC AATGGACTAT 
CTGGTGAAAA ACACGTTCAC TCAGGCGGCT GGAGCCAGGA AAGGCTTCCC AAAAGTGGCC 
ATGATCATCA CAGATGGAAA ATCTCAAGAC CCCGTAGAGG AGTACGCCGA ACGGTTGAGG 
AACATCGGAG TGGAGATCTT TGTGCTGGGT ATCAAAGGAG CAGATGAGGA CGAGCTGAAG 
GAGATCGCCT CCAGACCCCA TAGCAAGCAT GTCTATAACG TCCCCAACTT CGACATGATC 
AATCAGGTCC AGAAGGCGCT CATCACTGAA GTTTGCTCTG GCGTGGAGGA GCAGCTAAAT 
TCCCTTGTAA GCGGGGAGGA AGTTGTCGAA CCAGCTTCCA ACCTCAGAGT GACAGATATC 
TCCTCCAAAT CCATGAGAAT TGCGTGGGAT TCATCTCCTG GTGATATCAC AGGCTATAAG 
CTGCAGGTTT TTCCCACTAT TGCGAGCGCC AAAAAGCAGG AGCTCTACAC TGGGCCCACG 
ATGACCTTGG CCAATGTCAG AGACCTGACG CCTGAGATGG AGTATGAGAT CAGTCTGTTT 
GCTCTCAAAG GTCTGACTCC TAGTGTGCCG CAAGTTGTCA CTGAGAAAAC TCAGCCAGTG 
AAAGTATTTA CAGAGTGTTC CCTAGAGGTG GATGTCCAAG CTGACATTGT CCTCCTGGTG 
GATGGGTCAT ATAGTATAGG ACTGCCTAAC TTTGCGAAGG TTAGAGCTTT CCTCGAAGTA 
CTTGTGAATT CTTTTGACAT CGGACCTAAC AAAGTGCAGA TCAGCTTGGT TCAGTACAGC 
AGGGATCCTC ACACTGAGTT CGCCTTGAAT AAATTCGATG ACAATGCAGC CATGGTCAAG 
GCGGTCCGCA CCTTCCCCTA CCGAGGTGGA TCCACCAACA CCGGCAAGGC CATGACATAT 
GTGAGGGAAA AGATCTTTGT TTCGGGCCGT GGCGCTCGTG ATAACGTCCC CCGAGTCATG 
GTGCTTATCA CAGACGGCAA GTCCTCAGAT TCTTTCAAAG ACCCCGCCAA CAAACTGAGG 
GATACAGATG TGGAGATTTT TGCAGTGGGA GTAAAAGATG CCGTACGATC TGAGCTGGAG 
GCTATCGCCA ATGTTCCTGC TGATAACCAT GTGTTTGAGG TGGAGGACTT TGATGCTTTC 
GAGCGGATCT CCAAGGAGCT GACCGCGTCC ATCTGCCTCA GAATTGAGCA GGAGCTCCAT 
AACATCAGGA AAAGAAGTTT GCTTCCCCCA AAGTCACTGA GCTTCTCTGA GGTGACCTCC 
AGGAGTTTCA GGACCTCATG GGTTTCTGAT GGAGAGGATA TCCTGTCTTT TCTGGTGCGC 
TTCAGGCCAG CGGCGGACCT AACTGGAGAC TACATCTCTC TGGTGGTTCC CCATGACATC 
ACCAGTACAA TTCTGCCCAA TCTCACGCCA CTCACCACCT ACGAAGTTAA CGTCATTGCT 
CAGTATGATA AAGGAGACAG CTTTCCTTTG ACGGGCGAGG AGACCACTTT AGAAGAGTTG 
GGTTCTGTTC GTAACCTGAT GGTGAGCGAG GAGGCTGTGG ACCGCTTTCG GGTGTCGTGG 
AGAGCTGCTC CAGGATTAGT GCTGAGGTAC AGACTGCTCT ACGAGCCTGT GAGCGGAGGG 
GATAAACTGG AAGCAGAGAC TGATGGGAAT CAGGTCACCA CTGTGCTCAA TGAACTACTA 
CCGGTCACTA CTTACCGGGT CACCGTTTAC CCTGAGTACG CAAGTGGAAG GGGTCCCTCC 
ATGGACACTG AAGGCACCAC CAAAGAAGTT CGAGGTTCTC CACGCGACTT AAGGGTCTTC 
AATGAGACGG TGTCTAGCAT GAGGGTCTCG TGGCGGGCTG CCCCAGGCAA CGTGCTTCAA 
TATCAGGTGG CCTACAAACC TGACGGTGGG GAGAGGAAAG AAATAAGTGT TAAAGGAGAG 
ACAACAACTG CTGTGCTGAA AAATCTGCTG CCGGACACGG AGTATGAGCT GTTTGTTAGC 
GCACGTTACG TCTCTGGACT TGGCGCACCA CTTATGGGAA CAGGCACCAC GCTGGAAGAA 
CTTGGTTCAC CTTCGAATCT GGTAACTTCA GACGTCACTG AGAACAGTTT TGTAGCATCC 
TGGACCGCCG CCCCTGGAAA CGTCCGTCTT TATCGGGTCA CCTGGAAATC CCTCTTTTCA 
GAGGAAGCTG GCGAGAAGAC TGTCCCCGGG GACGTAACCA CCACTGTTCT GGACGGGCTG 
ACACCAGAGA CCCGCTACAA GGTTTCTGTC TACGCTGTCT ACAGCCGTGC GGAGGGGGCG 
CCACTGCACG GAGAGGAGAC CACAGATGTG TCGGCCGCTA CCAGAGCTCT TACGGTGTCA 
GAAGAAACAG AGAGGACCAT GAAGGTGACA TGGATACCAG CACCTGGCAA TGTTGTCAAT 
TACAGGGTAT CATACAAACC TCAGGGTGAT TTACGCCAAA TGGCCTCCAA AGCACCAGGA 
GGCACCAATT CTATAGTCCT GAGACGCCTG ATCCCCCAGA CCACTTACGA TATCACAGTG 
GTTCCCGTCT ACAAGTTTGG GGATGGAAGA CAAAGGCAAG GAGAAGGAAC TACATTGTCT 
CAATATAAAG CTCCCAGAAA CTTGCAAACT TCGGAGCCCA CTAAAACCAG TTTCAGGGTG 
ACCTGGGATC ATGCTCCTGG AGATGTAAAA GGCTACAAGG TCACTTTCCA TCCCACCGGA 
AAGGAAGTGG ACCTGGGAGA GTTACTCGTA GGCCCCTATG ACAACACTGT GGTTCTGGAG 
GAACTAAGGG CCGGCACTAA GTATACGGTA AATGTGTTTG GTATGTTTGA TGGAGGAGAG 
AGTATGCCAT TGGCTGGAGA GGAGAAGACC ACCCTCTCTG ACGCAGCTTC TGAAAACCTC 
CCTTATACAC CTTCAGATGC CCAGTGTAAA ACCACGGCCC AAGCGGATAT CGTCCTGCTT 
GTTGATGGAT CTTGGAGCAT TGGTCGCCTG AACTTCAAGA CCATACGCAA TTTCATTGCT 
CGCATGGTGG GAGTCTTCGA CATCGGCCCC GATCGTGTCC AGATTGGTCT GGCACAGTAC 
AGCGGAGACC CGAAGACTGA GTGGCATCTC AATGCTCACA GAACCAGGAC TCAGCTGCTG 
GATGCGGTGG CCAATCTACC CTACAAAGGA GGAAACACTC TCACCGGCCT TGCTTTGAAT 
TACATCCTTC AGAATAACTT CAAGCCTAAC GTTGGCATGC GTCCAAACTC CAGAAAGATC 
GGCGTCCTGG TCACTGATGG CAAGTCCCAA GACGACATTG TGGCGAACTC ACAGAACCTG 
CGGGACCAAG GGATTGAACT GTACGCCATT GGTGTAAAGA ACGCAGATGA GAATGAGTTG 
AGAACCATTG CTTCCGATCC CGATGACATT CACATGTACA ACGTGGCTGA TTTCTCCTTC 
CTGCTGGACA TTGTGGATGA CCTCACAAAC AACCTGTGCA ACAGCGTCAA AGGTCCAGGA 
GGAGGACCAG AGCCACCCAC TGATCTGACC ACCTCAGAGG TCACACATTA CTCTTTCCGT 
GCCACTTGGG TGGCTCCTGA CGGGCCTGTG GAGAAGTACA GAGTGGAGTA TGTGAGCACC 
GCTGGAGGAG AACCACAGCA ACAGATGTTT GTGGACGGGA CGGTGACCAC TGTGGTTTTG 
GAAGGTCTCA CTCCGCTGAC CGAGTACATA GTAAAGGTTT ACTCAGTGGT GGGAGAGGAC 
AGCAGCGACC CACTCAGAGG CATAGAGACC ACATTGCCTC TTCCTCCATC CGGTGAGTTG 
AGGATTACCG ATGTGACCCA CAGCACCATG CAGCTGGACT GGGACGCTGC GCCCGGCGCC 
GTCCGAAAGT ACATCATCAC TTACAAGCCA GAGGAGGGAG AGGCGAAGGA GCTTGAAGTT 
GACGGAGATG TAACATCTAG AGGCCTGGAT AATCTCATCT CCCAGACTGA ATATGATGTG 
GCTGTGACTC CGGTTTATGA TTCGGGCACT GGAAACCCCA TGCTTAACCA GGCCATTACA 
GATGTGGTTC CTGCCCCGCA GAACTTGAGG TTCTCGATGG TGACCCAGAC CAGTTTTAGG 
GTTCACTGGG AGCACGGCGC TCCTGATGTC TCCCTCTACC GTATTGGCTG GAAAAAGAAA 
GGAACGACCG AGTTTCAAAA TGATATCCTG AGTAGTGAAG ACACTACATA TGATCTGGTG 
GACCTGGAGC CAGACACACC TTATGATGTT TCGGTCACTG CCATTTACCC TGATGAGTCT 
GAGAGCGAGG ATCTGCTGGG CACTGAACGC ACTTTGGCCA AGCTTGCTAC ACCACCTCCC 
AGTGGTCCTC CTCAAAACCT CCAGGTGTTC GACGAGACCA CCACCTCCCT CACGACTAAG 
TGGGACCACG CGCCTGGACG AGTTCAGAAC TACAAAATCA CCTACGTACC GCTTACTGGA 
GGACGATCAC AGTCGACGCA GGTCGGAGGA AAGAAAAACA CAGTGATCCT GCAGAAACTG 
ACTCCAGACA CTCCTTACTC CATCACTGTT GCTGCGGTCT ACGCCAGCGG AGAGGCCAGA 
GACATTTCTG GCCAAGGAAA GACATTGCCG CTGGGCAGTG TCAGGAATCT TCAGGTCACT 
GACCCCACAG TTAGCACTCT GAATGTGCGC TGGGAGCCGG CGGAGGGAAG CGTTCGCCAG 
TACAGGATCT ACTACCAGCC CGTCGCAGGG GGCGCAGAGG ACATGGTCCA GGTTCCTGGA 
AGCACCACCA GCACCGTTCT GAAGAACCTG CAATCTGACA CTCAGTACAG AGTGACGGTG 
GTGCCAGTCT ACGCTCCTGG AGAGGGCAAG CGCATGTCGG AGACCGGAAA AACACTGGTT 
CGGACCCCTG CCAAGAACAT CCAGGTTTAC AACCCCACCA CAAACACCCT GAACGTGCGC 
TGGGAGCCGG CCTCAGGACA GGTGCAGCAG TACCGCGTGG CTTACGCCCC TCTCTCCGGG 
ATCCGACCCT CTCAGTCGGT GCTGGTTCCA GGAAACACCC ACACGGCTCT GCTGCAGCAG 
CTGGAATCAG ACACTGACTA CTCTGTGAGT GTGGTGGCTC TGTATGCTGA TGGAGAGGGC 
TCGTCTGTGT CTGATAATGG CAAAACACTG CCCCGCAGTG GCCCCAGGAA CCTGCGGGTT 
TATGATCCCA CCACCAGCAC GCTGTCGGTG GGCTGGGAAC ATGCAGAAGG GCCTGTGATT 
CAGTACCGCA TTGGCTACGC ACCCACCACT GGAGATCCCA TTGAAGAATT TACAACTGTC 
CCAGGCAACC GTAACTCGGT CATCCTGCAA AACCTGCAGC CAGACACGCC GTACAACATC 
AACGTAGAAG CCATTTATCA AGATGGCCCT GGAGGACGAC TCAGTGGAGA TGGAAAAACA 
TTGGATCTCC TTGGGCCTCG TAACCTGCGT GTTTCAGACG AGTGGTACAC ACGTTTCCGA 
GTGTCCTGGG ATCCTGCAGC CGCACGGGTG AATGGATATA AACTGGTCTA TACACCAGTG 
GGCACAACCG ACACCATGGA AGCCTTCGTT GGAGACGTGA CTTCACACCA CCTGAACAAC 
CTCCAGCCTG GCACCACATA CGACCTGAAG GTTTACGCTC AGTATGATGC GGGCTTGAGC 
GGCGCTCTGG TCGGACAGGG AACCACCCTG TATCTCAACG TGACCGACCT CACTTCCTAC 
AACGTGGGCT ACGACTCTTT CTGCATCAGA TGGACTCCTC ATCGAGCTGC CACCTCCTAC 
AGACTCAAAG TCAACCCTTT CAACCCATCT CACCGTGGAG CTCAGGAGGT CACGATCACT 
GCGGCTGAGA GCCAGTACTG TTTTGACGGC CTGACGCCAG ACGCGCTATA CAACGCCACC 
GTCTACACGC AGACCCCCAA CCGAGAGGGG CCCGGAGTCA GTGTCAAAGA GCGCACACTG 
GTGAAGCCCA CCGTCGCCCC AACACTACCT CCAACTCCTC CACCTCCAGC CACCATTCCT 
TCTGCACTCG AGGTGTGCAA AGGTGCCAAG GCTGACCTGG TGTTCCTGAT CGATGGCTCG 
TGGAGTATCG GAGATGACAG CTTCGCTAAA GTCCGGCAGT TTGTGTTCAG CATGATTGGG 
GCGTTTGATG TGGTCAGCCA TGAGGGCATG CAGGTGTCTT TCGTGCAGTA CAGCGACGAC 
GCCAAAACTG AGTTCAAGCT CAACACCTAC CACGACAAGG GAATGGTGCT GTCTGCTCTT 
CAGATGGTCA ATTATAGAGG AGGAAACACC AAAACAGGTG CTGCACTGAA GCATGTTGGA 
GAAAAAGTGT TCACCTCGGA CAATGGCATG AGGAGAACCG TTCCCAAGGT ACTGGTTGTG 
GTGACCGATG GACGTTCTCA AGATGACGTG AAGAAGAGCG CTGCTAAACT ACAGCATGCA 
GGTTACAGTG TGTTTGTGGT GGGTGTCGCT GACATTGACG TTAATGAACT GAGGAACATT 
GGAAGCAAGC CCAGTGACAG ACACGTCTTT ATCGTCGACA ACTTCGATGC TTTCGCCAAG 
ATCCAAGACA ACCTGATCAC GTTCATCTGT GAAACGGCAA CGTCCACATG CCCTTTGATT 
TACCTCAATG GATTCACATC ACCAGGCTTC AGGATGCTGG AAGCTTTCAA CATCACTGAC 
AAGACCTACG CAGGCATGAA CGGCGTCTCT CTGGAAACCG GCTCCTTCAA CAGCTTCATC 
GCATACAGAC TCCATAAGGA CGCCTTCATT TCCCAGCCAA CAAAGGAAAT CCATCCAGAT 
GGTCTTCCTC CATCCTACAC CCTCATCCTC CTCTTCCGTC TGCTCCCGGA CACCGGCAAG 
GATGCGTTTG ATTTATGGCA GATCTCAGAT AAGAACAATA ACCCTGAGGT CGGGCTCACC 
ATTGACCCCT CCAGCAGAGT GATCTCCTTC TACAATAAAG ACACTAGAGG GGAAATTCAG 
AAAGTCTCCT TTGATAACGA CCACGTGAAG AAGATTTTCC ACGGCAGTTT CCACAAGCTT 
CACATTTCTA TTTCGCCGAA AAATGTCAAA CTCAACATCG ACTGCCAAGA GGTGGCTGAG 
AAACAGATCA AAGCAGCCAA CAACGTCTCC ACAGATGGCT ACGAAGTCCT TGGAAAAATG 
ACAAAGTCTG GCGGTTCAAA GAAGCAGTCG GCCACATTCC AGCTCCAGAT GTTTGATATT 
GTCTGCAGTT TGGGCTGGAC AAGTCGAGAC AAATGTTGTG ATATTCCAGC AATGCGAGAT 
GAAGCCAAGT GTCCTGCTCT TCCTCACTCC TGCACATGTG CTCAGGACAG TGTTGGTCCT 
CCAGGCCCAG CGGGTCCTCA AGGCAGTCCT GGTACAAAAG GCCCACGAGG CGAGAGAGGA 
GAGTCTGGTC CTACGGGTCC AATGGGTCCA CGTGGAGAGC AGGGTCTGCC AGGGCAGATG 
GGGCCACCGG GTCCACAGGG CCCCAATGGA CTATCCCTTC CCGGAGAACC AGGGCGACCG 
GGACCTAAGG GAGACCCCGG TGACTCAGGT TTACCTGGAC AGAGAGGCCC GGTTGGCGCT 
GCAGGTCCAC TCGGCCCTGT TGGACCCGCT GGAGCAAGAG GTCCACCAGG AAGTGAAGGC 
CCATCTGGAC CAAGAGGTCC ATCGGGACCA ATGGGACCAC CGGGAGCTCC CGGAATGCCA 
GGAATCACCG GAAAACCAGG AAAACCCGGA GACACTGGCT TTCCTGGACC TGTTGGCATG 
AAAGGAGAGA AGGGTGAAAG AGGAGACACT GCGTCACAGA ATATGATGCG CTCTGTAGCG 
AGACAAGTCT GCGAACAGCT AGTCAGCAGA CAAATGAGCA GAATTGATAT GATGATGAAC 
CAGATCCCCA GCAACTACAG GAGTAACAGC CCCGGCCCCG CTGGACCTCC AGGCCCACCT 
GGAAACCAGG GACCCCATGG AGAGCCTGGT CAGCCCGGAC CCAATGGCTT CCCAGGAAAT 
CCAGGTTTAC CAGGCTCTCC TGGAGAAAGA GGAGTGCCTG GAGAGAAGGG TGAACGAGGC 
AGCCCCGGCA TTGGCACTCG AGGACAGAGA GGATTACCTG GACCTCCTGG GCCACCAGGG 
CAGTCTCAGA CCGGTCCCCC TGGTCCCTCA GGCTCACCCG GGCCCCGAGG ACCACCAGGA 
CAACATGGTT CTTCTGGAGT CACAGGACCA CCAGGACCTC CAGGATACTG TGACTCCTCT 
CAGTGCGGCA TGCCTTACAA CGGACAAGGC TACCCAGGCC CCTACCAGCC CGGGTCTGAA 
ACCTACGTAG TGCCCATACC AGCCGCGCCA TCGGAAGATA TCGAGACACA GTCGCCAGGA 
TTGCGCTACC AGCGCGGAAA AAGGTCGCTA TCAAGAAAGG AAGCCGAAAG AATCAAAACA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291572.1
CDS	179..9241
Translation	MKIRLSLAAAALLTLVLSSVEAQVEPPSDLKFKILNENTVQMSWRKPSSQIQGYRIQVTSDTDDSKELSLPASSTSTSITDLTPDVDYSVSINSYDGAEESIPILGQITIQSSNTSGPRRPQSEAVKCSVSAVADLVFLVDGSWSVGRENFRFIRSFIGAMAGAFDIEEDKTRVAVVQYSSDTRTEFSLNTHFRRPDVLRAIKNLPYKGGNTMTGDAMDYLVKNTFTQAAGARKGFPKVAMIITDGKSQDPVEEYAERLRNIGVEIFVLGIKGADEDELKEIASRPHSKHVYNVPNFDMINQVQKALITEVCSGVEEQLNSLVSGEEVVEPASNLRVTDISSKSMRIAWDSSPGDITGYKLQVFPTIASAKKQELYTGPTMTLANVRDLTPEMEYEISLFALKGLTPSVPQVVTEKTQPVKVFTECSLEVDVQADIVLLVDGSYSIGLPNFAKVRAFLEVLVNSFDIGPNKVQISLVQYSRDPHTEFALNKFDDNAAMVKAVRTFPYRGGSTNTGKAMTYVREKIFVSGRGARDNVPRVMVLITDGKSSDSFKDPANKLRDTDVEIFAVGVKDAVRSELEAIANVPADNHVFEVEDFDAFERISKELTASICLRIEQELHNIRKRSLLPPKSLSFSEVTSRSFRTSWVSDGEDILSFLVRFRPAADLTGDYISLVVPHDITSTILPNLTPLTTYEVNVIAQYDKGDSFPLTGEETTLEELGSVRNLMVSEEAVDRFRVSWRAAPGLVLRYRLLYEPVSGGDKLEAETDGNQVTTVLNELLPVTTYRVTVYPEYASGRGPSMDTEGTTKEVRGSPRDLRVFNETVSSMRVSWRAAPGNVLQYQVAYKPDGGERKEISVKGETTTAVLKNLLPDTEYELFVSARYVSGLGAPLMGTGTTLEELGSPSNLVTSDVTENSFVASWTAAPGNVRLYRVTWKSLFSEEAGEKTVPGDVTTTVLDGLTPETRYKVSVYAVYSRAEGAPLHGEETTDVSAATRALTVSEETERTMKVTWIPAPGNVVNYRVSYKPQGDLRQMASKAPGGTNSIVLRRLIPQTTYDITVVPVYKFGDGRQRQGEGTTLSQYKAPRNLQTSEPTKTSFRVTWDHAPGDVKGYKVTFHPTGKEVDLGELLVGPYDNTVVLEELRAGTKYTVNVFGMFDGGESMPLAGEEKTTLSDAASENLPYTPSDAQCKTTAQADIVLLVDGSWSIGRLNFKTIRNFIARMVGVFDIGPDRVQIGLAQYSGDPKTEWHLNAHRTRTQLLDAVANLPYKGGNTLTGLALNYILQNNFKPNVGMRPNSRKIGVLVTDGKSQDDIVANSQNLRDQGIELYAIGVKNADENELRTIASDPDDIHMYNVADFSFLLDIVDDLTNNLCNSVKGPGGGPEPPTDLTTSEVTHYSFRATWVAPDGPVEKYRVEYVSTAGGEPQQQMFVDGTVTTVVLEGLTPLTEYIVKVYSVVGEDSSDPLRGIETTLPLPPSGELRITDVTHSTMQLDWDAAPGAVRKYIITYKPEEGEAKELEVDGDVTSRGLDNLISQTEYDVAVTPVYDSGTGNPMLNQAITDVVPAPQNLRFSMVTQTSFRVHWEHGAPDVSLYRIGWKKKGTTEFQNDILSSEDTTYDLVDLEPDTPYDVSVTAIYPDESESEDLLGTERTLAKLATPPPSGPPQNLQVFDETTTSLTTKWDHAPGRVQNYKITYVPLTGGRSQSTQVGGKKNTVILQKLTPDTPYSITVAAVYASGEARDISGQGKTLPLGSVRNLQVTDPTVSTLNVRWEPAEGSVRQYRIYYQPVAGGAEDMVQVPGSTTSTVLKNLQSDTQYRVTVVPVYAPGEGKRMSETGKTLVRTPAKNIQVYNPTTNTLNVRWEPASGQVQQYRVAYAPLSGIRPSQSVLVPGNTHTALLQQLESDTDYSVSVVALYADGEGSSVSDNGKTLPRSGPRNLRVYDPTTSTLSVGWEHAEGPVIQYRIGYAPTTGDPIEEFTTVPGNRNSVILQNLQPDTPYNINVEAIYQDGPGGRLSGDGKTLDLLGPRNLRVSDEWYTRFRVSWDPAAARVNGYKLVYTPVGTTDTMEAFVGDVTSHHLNNLQPGTTYDLKVYAQYDAGLSGALVGQGTTLYLNVTDLTSYNVGYDSFCIRWTPHRAATSYRLKVNPFNPSHRGAQEVTITAAESQYCFDGLTPDALYNATVYTQTPNREGPGVSVKERTLVKPTVAPTLPPTPPPPATIPSALEVCKGAKADLVFLIDGSWSIGDDSFAKVRQFVFSMIGAFDVVSHEGMQVSFVQYSDDAKTEFKLNTYHDKGMVLSALQMVNYRGGNTKTGAALKHVGEKVFTSDNGMRRTVPKVLVVVTDGRSQDDVKKSAAKLQHAGYSVFVVGVADIDVNELRNIGSKPSDRHVFIVDNFDAFAKIQDNLITFICETATSTCPLIYLNGFTSPGFRMLEAFNITDKTYAGMNGVSLETGSFNSFIAYRLHKDAFISQPTKEIHPDGLPPSYTLILLFRLLPDTGKDAFDLWQISDKNNNPEVGLTIDPSSRVISFYNKDTRGEIQKVSFDNDHVKKIFHGSFHKLHISISPKNVKLNIDCQEVAEKQIKAANNVSTDGYEVLGKMTKSGGSKKQSATFQLQMFDIVCSLGWTSRDKCCDIPAMRDEAKCPALPHSCTCAQDSVGPPGPAGPQGSPGTKGPRGERGESGPTGPMGPRGEQGLPGQMGPPGPQGPNGLSLPGEPGRPGPKGDPGDSGLPGQRGPVGAAGPLGPVGPAGARGPPGSEGPSGPRGPSGPMGPPGAPGMPGITGKPGKPGDTGFPGPVGMKGEKGERGDTASQNMMRSVARQVCEQLVSRQMSRIDMMMNQIPSNYRSNSPGPAGPPGPPGNQGPHGEPGQPGPNGFPGNPGLPGSPGERGVPGEKGERGSPGIGTRGQRGLPGPPGPPGQSQTGPPGPSGSPGPRGPPGQHGSSGVTGPPGPPGYCDSSQCGMPYNGQGYPGPYQPGSETYVVPIPAAPSEDIETQSPGLRYQRGKRSLSRKEAERIKT

Target ORF information:

RefSeq Version	XM_009293297.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type XII, alpha 1a (col12a1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293297.3

ORF Insert Sequence:

ATGAAGATCA GGTTGTCTTT GGCCGCTGCT GCGCTCTTGA CTCTAGTACT CAGTTCAGTA 
GAGGCTCAAG TGGAGCCTCC GTCAGACTTA AAATTTAAAA TCCTGAATGA AAACACAGTG 
CAAATGTCAT GGAGGAAGCC CTCGTCTCAG ATACAGGGCT ACAGAATACA GGTCACGTCA 
GATACAGATG ATTCTAAAGA ATTGAGTCTT CCTGCCTCGT CCACAAGCAC ATCAATTACA 
GATCTAACTC CAGATGTAGA CTATTCAGTG TCAATCAATT CATATGATGG TGCTGAAGAA 
AGTATCCCTA TCCTTGGACA AATCACAATT CAATCCAGCA ACACAAGTGG ACCAAGGAGG 
CCACAGTCAG AGGCTGTCAA GTGTTCAGTG AGTGCCGTTG CGGATCTGGT TTTCCTGGTG 
GACGGCTCTT GGAGTGTGGG AAGAGAAAAC TTTAGATTTA TCCGGAGTTT CATTGGGGCC 
ATGGCCGGGG CCTTCGACAT TGAAGAGGAT AAGACCAGAG TGGCTGTGGT TCAGTACAGC 
TCGGACACCA GGACAGAGTT CAGTCTAAAC ACACATTTCA GACGACCCGA TGTCCTGCGA 
GCCATTAAAA ATCTGCCGTA CAAGGGTGGA AATACCATGA CGGGTGATGC AATGGACTAT 
CTGGTGAAAA ACACGTTCAC TCAGGCGGCT GGAGCCAGGA AAGGCTTCCC AAAAGTGGCC 
ATGATCATCA CAGATGGAAA ATCTCAAGAC CCCGTAGAGG AGTACGCCGA ACGGTTGAGG 
AACATCGGAG TGGAGATCTT TGTGCTGGGT ATCAAAGGAG CAGATGAGGA CGAGCTGAAG 
GAGATCGCCT CCAGACCCCA TAGCAAGCAT GTCTATAACG TCCCCAACTT CGACATGATC 
AATCAGGTCC AGAAGGCGCT CATCACTGAA GTTTGCTCTG GCGTGGAGGA GCAGCTAAAT 
TCCCTTGTAA GCGGGGAGGA AGTTGTCGAA CCAGCTTCCA ACCTCAGAGT GACAGATATC 
TCCTCCAAAT CCATGAGAAT TGCGTGGGAT TCATCTCCTG GTGATATCAC AGGCTATAAG 
CTGCAGGTTT TTCCCACTAT TGCGAGCGCC AAAAAGCAGG AGCTCTACAC TGGGCCCACG 
ATGACCTTGG CCAATGTCAG AGACCTGACG CCTGAGATGG AGTATGAGAT CAGTCTGTTT 
GCTCTCAAAG GTCTGACTCC TAGTGTGCCG CAAGTTGTCA CTGAGAAAAC TCAGCCAGTG 
AAAGTATTTA CAGAGTGTTC CCTAGAGGTG GATGTCCAAG CTGACATTGT CCTCCTGGTG 
GATGGGTCAT ATAGTATAGG ACTGCCTAAC TTTGCGAAGG TTAGAGCTTT CCTCGAAGTA 
CTTGTGAATT CTTTTGACAT CGGACCTAAC AAAGTGCAGA TCAGCTTGGT TCAGTACAGC 
AGGGATCCTC ACACTGAGTT CGCCTTGAAT AAATTCGATG ACAATGCAGC CATGGTCAAG 
GCGGTCCGCA CCTTCCCCTA CCGAGGTGGA TCCACCAACA CCGGCAAGGC CATGACATAT 
GTGAGGGAAA AGATCTTTGT TTCGGGCCGT GGCGCTCGTG ATAACGTCCC CCGAGTCATG 
GTGCTTATCA CAGACGGCAA GTCCTCAGAT TCTTTCAAAG ACCCCGCCAA CAAACTGAGG 
GATACAGATG TGGAGATTTT TGCAGTGGGA GTAAAAGATG CCGTACGATC TGAGCTGGAG 
GCTATCGCCA ATGTTCCTGC TGATAACCAT GTGTTTGAGG TGGAGGACTT TGATGCTTTC 
GAGCGGATCT CCAAGGAGCT GACCGCGTCC ATCTGCCTCA GAATTGAGCA GGAGCTCCAT 
AACATCAGGA AAAGAAGTTT GCTTCCCCCA AAGTCACTGA GCTTCTCTGA GGTGACCTCC 
AGGAGTTTCA GGACCTCATG GGTTTCTGAT GGAGAGGATA TCCTGTCTTT TCTGGTGCGC 
TTCAGGCCAG CGGCGGACCT AACTGGAGAC TACATCTCTC TGGTGGTTCC CCATGACATC 
ACCAGTACAA TTCTGCCCAA TCTCACGCCA CTCACCACCT ACGAAGTTAA CGTCATTGCT 
CAGTATGATA AAGGAGACAG CTTTCCTTTG ACGGGCGAGG AGACCACTTT AGAAGAGTTG 
GGTTCTGTTC GTAACCTGAT GGTGAGCGAG GAGGCTGTGG ACCGCTTTCG GGTGTCGTGG 
AGAGCTGCTC CAGGATTAGT GCTGAGGTAC AGACTGCTCT ACGAGCCTGT GAGCGGAGGG 
GATAAACTGG AAGCAGAGAC TGATGGGAAT CAGGTCACCA CTGTGCTCAA TGAACTACTA 
CCGGTCACTA CTTACCGGGT CACCGTTTAC CCTGAGTACG CAAGTGGAAG GGGTCCCTCC 
ATGGACACTG AAGGCACCAC CAAAGAAGTT CGAGGTTCTC CACGCGACTT AAGGGTCTTC 
AATGAGACGG TGTCTAGCAT GAGGGTCTCG TGGCGGGCTG CCCCAGGCAA CGTGCTTCAA 
TATCAGGTGG CCTACAAACC TGACGGTGGG GAGAGGAAAG AAATAAGTGT TAAAGGAGAG 
ACAACAACTG CTGTGCTGAA AAATCTGCTG CCGGACACGG AGTATGAGCT GTTTGTTAGC 
GCACGTTACG TCTCTGGACT TGGCGCACCA CTTATGGGAA CAGGCACCAC GCTGGAAGAA 
CTTGGTTCAC CTTCGAATCT GGTAACTTCA GACGTCACTG AGAACAGTTT TGTAGCATCC 
TGGACCGCCG CCCCTGGAAA CGTCCGTCTT TATCGGGTCA CCTGGAAATC CCTCTTTTCA 
GAGGAAGCTG GCGAGAAGAC TGTCCCCGGG GACGTAACCA CCACTGTTCT GGACGGGCTG 
ACACCAGAGA CCCGCTACAA GGTTTCTGTC TACGCTGTCT ACAGCCGTGC GGAGGGGGCG 
CCACTGCACG GAGAGGAGAC CACAGATGTG TCGGCCGCTA CCAGAGCTCT TACGGTGTCA 
GAAGAAACAG AGAGGACCAT GAAGGTGACA TGGATACCAG CACCTGGCAA TGTTGTCAAT 
TACAGGGTAT CATACAAACC TCAGGGTGAT TTACGCCAAA TGGCCTCCAA AGCACCAGGA 
GGCACCAATT CTATAGTCCT GAGACGCCTG ATCCCCCAGA CCACTTACGA TATCACAGTG 
GTTCCCGTCT ACAAGTTTGG GGATGGAAGA CAAAGGCAAG GAGAAGGAAC TACATTGTCT 
CAATATAAAG CTCCCAGAAA CTTGCAAACT TCGGAGCCCA CTAAAACCAG TTTCAGGGTG 
ACCTGGGATC ATGCTCCTGG AGATGTAAAA GGCTACAAGG TCACTTTCCA TCCCACCGGA 
AAGGAAGTGG ACCTGGGAGA GTTACTCGTA GGCCCCTATG ACAACACTGT GGTTCTGGAG 
GAACTAAGGG CCGGCACTAA GTATACGGTA AATGTGTTTG GTATGTTTGA TGGAGGAGAG 
AGTATGCCAT TGGCTGGAGA GGAGAAGACC ACCCTCTCTG ACGCAGCTTC TGAAAACCTC 
CCTTATACAC CTTCAGATGC CCAGTGTAAA ACCACGGCCC AAGCGGATAT CGTCCTGCTT 
GTTGATGGAT CTTGGAGCAT TGGTCGCCTG AACTTCAAGA CCATACGCAA TTTCATTGCT 
CGCATGGTGG GAGTCTTCGA CATCGGCCCC GATCGTGTCC AGATTGGTCT GGCACAGTAC 
AGCGGAGACC CGAAGACTGA GTGGCATCTC AATGCTCACA GAACCAGGAC TCAGCTGCTG 
GATGCGGTGG CCAATCTACC CTACAAAGGA GGAAACACTC TCACCGGCCT TGCTTTGAAT 
TACATCCTTC AGAATAACTT CAAGCCTAAC GTTGGCATGC GTCCAAACTC CAGAAAGATC 
GGCGTCCTGG TCACTGATGG CAAGTCCCAA GACGACATTG TGGCGAACTC ACAGAACCTG 
CGGGACCAAG GGATTGAACT GTACGCCATT GGTGTAAAGA ACGCAGATGA GAATGAGTTG 
AGAACCATTG CTTCCGATCC CGATGACATT CACATGTACA ACGTGGCTGA TTTCTCCTTC 
CTGCTGGACA TTGTGGATGA CCTCACAAAC AACCTGTGCA ACAGCGTCAA AGGTCCAGGA 
GGAGGACCAG AGCCACCCAC TGATCTGACC ACCTCAGAGG TCACACATTA CTCTTTCCGT 
GCCACTTGGG TGGCTCCTGA CGGGCCTGTG GAGAAGTACA GAGTGGAGTA TGTGAGCACC 
GCTGGAGGAG AACCACAGCA ACAGATGTTT GTGGACGGGA CGGTGACCAC TGTGGTTTTG 
GAAGGTCTCA CTCCGCTGAC CGAGTACATA GTAAAGGTTT ACTCAGTGGT GGGAGAGGAC 
AGCAGCGACC CACTCAGAGG CATAGAGACC ACATTGCCTC TTCCTCCATC CGGTGAGTTG 
AGGATTACCG ATGTGACCCA CAGCACCATG CAGCTGGACT GGGACGCTGC GCCCGGCGCC 
GTCCGAAAGT ACATCATCAC TTACAAGCCA GAGGAGGGAG AGGCGAAGGA GCTTGAAGTT 
GACGGAGATG TAACATCTAG AGGCCTGGAT AATCTCATCT CCCAGACTGA ATATGATGTG 
GCTGTGACTC CGGTTTATGA TTCGGGCACT GGAAACCCCA TGCTTAACCA GGCCATTACA 
GATGTGGTTC CTGCCCCGCA GAACTTGAGG TTCTCGATGG TGACCCAGAC CAGTTTTAGG 
GTTCACTGGG AGCACGGCGC TCCTGATGTC TCCCTCTACC GTATTGGCTG GAAAAAGAAA 
GGAACGACCG AGTTTCAAAA TGATATCCTG AGTAGTGAAG ACACTACATA TGATCTGGTG 
GACCTGGAGC CAGACACACC TTATGATGTT TCGGTCACTG CCATTTACCC TGATGAGTCT 
GAGAGCGAGG ATCTGCTGGG CACTGAACGC ACTTTGGCCA AGCTTGCTAC ACCACCTCCC 
AGTGGTCCTC CTCAAAACCT CCAGGTGTTC GACGAGACCA CCACCTCCCT CACGACTAAG 
TGGGACCACG CGCCTGGACG AGTTCAGAAC TACAAAATCA CCTACGTACC GCTTACTGGA 
GGACGATCAC AGTCGACGCA GGTCGGAGGA AAGAAAAACA CAGTGATCCT GCAGAAACTG 
ACTCCAGACA CTCCTTACTC CATCACTGTT GCTGCGGTCT ACGCCAGCGG AGAGGCCAGA 
GACATTTCTG GCCAAGGAAA GACATTGCCG CTGGGCAGTG TCAGGAATCT TCAGGTCACT 
GACCCCACAG TTAGCACTCT GAATGTGCGC TGGGAGCCGG CGGAGGGAAG CGTTCGCCAG 
TACAGGATCT ACTACCAGCC CGTCGCAGGG GGCGCAGAGG ACATGGTCCA GGTTCCTGGA 
AGCACCACCA GCACCGTTCT GAAGAACCTG CAATCTGACA CTCAGTACAG AGTGACGGTG 
GTGCCAGTCT ACGCTCCTGG AGAGGGCAAG CGCATGTCGG AGACCGGAAA AACACTGGTT 
CGGACCCCTG CCAAGAACAT CCAGGTTTAC AACCCCACCA CAAACACCCT GAACGTGCGC 
TGGGAGCCGG CCTCAGGACA GGTGCAGCAG TACCGCGTGG CTTACGCCCC TCTCTCCGGG 
ATCCGACCCT CTCAGTCGGT GCTGGTTCCA GGAAACACCC ACACGGCTCT GCTGCAGCAG 
CTGGAATCAG ACACTGACTA CTCTGTGAGT GTGGTGGCTC TGTATGCTGA TGGAGAGGGC 
TCGTCTGTGT CTGATAATGG CAAAACACTG CCCCGCAGTG GCCCCAGGAA CCTGCGGGTT 
TATGATCCCA CCACCAGCAC GCTGTCGGTG GGCTGGGAAC ATGCAGAAGG GCCTGTGATT 
CAGTACCGCA TTGGCTACGC ACCCACCACT GGAGATCCCA TTGAAGAATT TACAACTGTC 
CCAGGCAACC GTAACTCGGT CATCCTGCAA AACCTGCAGC CAGACACGCC GTACAACATC 
AACGTAGAAG CCATTTATCA AGATGGCCCT GGAGGACGAC TCAGTGGAGA TGGAAAAACA 
TTGGATCTCC TTGGGCCTCG TAACCTGCGT GTTTCAGACG AGTGGTACAC ACGTTTCCGA 
GTGTCCTGGG ATCCTGCAGC CGCACGGGTG AATGGATATA AACTGGTCTA TACACCAGTG 
GGCACAACCG ACACCATGGA AGCCTTCGTT GGAGACGTGA CTTCACACCA CCTGAACAAC 
CTCCAGCCTG GCACCACATA CGACCTGAAG GTTTACGCTC AGTATGATGC GGGCTTGAGC 
GGCGCTCTGG TCGGACAGGG AACCACCCTG TATCTCAACG TGACCGACCT CACTTCCTAC 
AACGTGGGCT ACGACTCTTT CTGCATCAGA TGGACTCCTC ATCGAGCTGC CACCTCCTAC 
AGACTCAAAG TCAACCCTTT CAACCCATCT CACCGTGGAG CTCAGGAGGT CACGATCACT 
GCGGCTGAGA GCCAGTACTG TTTTGACGGC CTGACGCCAG ACGCGCTATA CAACGCCACC 
GTCTACACGC AGACCCCCAA CCGAGAGGGG CCCGGAGTCA GTGTCAAAGA GCGCACACTG 
GTGAAGCCCA CCGTCGCCCC AACACTACCT CCAACTCCTC CACCTCCAGC CACCATTCCT 
TCTGCACTCG AGGTGTGCAA AGGTGCCAAG GCTGACCTGG TGTTCCTGAT CGATGGCTCG 
TGGAGTATCG GAGATGACAG CTTCGCTAAA GTCCGGCAGT TTGTGTTCAG CATGATTGGG 
GCGTTTGATG TGGTCAGCCA TGAGGGCATG CAGGTGTCTT TCGTGCAGTA CAGCGACGAC 
GCCAAAACTG AGTTCAAGCT CAACACCTAC CACGACAAGG GAATGGTGCT GTCTGCTCTT 
CAGATGGTCA ATTATAGAGG AGGAAACACC AAAACAGGTG CTGCACTGAA GCATGTTGGA 
GAAAAAGTGT TCACCTCGGA CAATGGCATG AGGAGAACCG TTCCCAAGGT ACTGGTTGTG 
GTGACCGATG GACGTTCTCA AGATGACGTG AAGAAGAGCG CTGCTAAACT ACAGCATGCA 
GGTTACAGTG TGTTTGTGGT GGGTGTCGCT GACATTGACG TTAATGAACT GAGGAACATT 
GGAAGCAAGC CCAGTGACAG ACACGTCTTT ATCGTCGACA ACTTCGATGC TTTCGCCAAG 
ATCCAAGACA ACCTGATCAC GTTCATCTGT GAAACGGCAA CGTCCACATG CCCTTTGATT 
TACCTCAATG GATTCACATC ACCAGGCTTC AGGATGCTGG AAGCTTTCAA CATCACTGAC 
AAGACCTACG CAGGCATGAA CGGCGTCTCT CTGGAAACCG GCTCCTTCAA CAGCTTCATC 
GCATACAGAC TCCATAAGGA CGCCTTCATT TCCCAGCCAA CAAAGGAAAT CCATCCAGAT 
GGTCTTCCTC CATCCTACAC CCTCATCCTC CTCTTCCGTC TGCTCCCGGA CACCGGCAAG 
GATGCGTTTG ATTTATGGCA GATCTCAGAT AAGAACAATA ACCCTGAGGT CGGGCTCACC 
ATTGACCCCT CCAGCAGAGT GATCTCCTTC TACAATAAAG ACACTAGAGG GGAAATTCAG 
AAAGTCTCCT TTGATAACGA CCACGTGAAG AAGATTTTCC ACGGCAGTTT CCACAAGCTT 
CACATTTCTA TTTCGCCGAA AAATGTCAAA CTCAACATCG ACTGCCAAGA GGTGGCTGAG 
AAACAGATCA AAGCAGCCAA CAACGTCTCC ACAGATGGCT ACGAAGTCCT TGGAAAAATG 
ACAAAGTCTG GCGGTTCAAA GAAGCAGTCG GCCACATTCC AGCTCCAGAT GTTTGATATT 
GTCTGCAGTT TGGGCTGGAC AAGTCGAGAC AAATGTTGTG ATATTCCAGC AATGCGAGAT 
GAAGCCAAGT GTCCTGCTCT TCCTCACTCC TGCACATGTG CTCAGGACAG TGTTGGTCCT 
CCAGGCCCAG CGGGTCCTCA AGGCAGTCCT GGTACAAAAG GCCCACGAGG CGAGAGAGGA 
GAGTCTGGTC CTACGGGTCC AATGGGTCCA CGTGGAGAGC AGGGTCTGCC AGGGCAGATG 
GGGCCACCGG GTCCACAGGG CCCCAATGGA CTATCCCTTC CCGGAGAACC AGGGCGACCG 
GGACCTAAGG GAGACCCCGG TGACTCAGGT TTACCTGGAC AGAGAGGCCC GGTTGGCGCT 
GCAGGTCCAC TCGGCCCTGT TGGACCCGCT GGAGCAAGAG GTCCACCAGG AAGTGAAGGC 
CCATCTGGAC CAAGAGGTCC ATCGGGACCA ATGGGACCAC CGGGAGCTCC CGGAATGCCA 
GGAATCACCG GAAAACCAGG AAAACCCGGA GACACTGGCT TTCCTGGACC TGTTGGCATG 
AAAGGAGAGA AGGGTGAAAG AGGAGACACT GCGTCACAGA ATATGATGCG CTCTGTAGCG 
AGACAAGTCT GCGAACAGCT AGTCAGCAGA CAAATGAGCA GAATTGATAT GATGATGAAC 
CAGATCCCCA GCAACTACAG GAGTAACAGC CCCGGCCCCG CTGGACCTCC AGGCCCACCT 
GGAAACCAGG GACCCCATGG AGAGCCTGGT CAGCCCGGAC CCAATGGCTT CCCAGGAAAT 
CCAGGTTTAC CAGGCTCTCC TGGAGAAAGA GGAGTGCCTG GAGAGAAGGG TGAACGAGGC 
AGCCCCGGCA TTGGCACTCG AGGACAGAGA GGATTACCTG GACCTCCTGG GCCACCAGGG 
CAGTCTCAGA CCGGTCCCCC TGGTCCCTCA GGCTCACCCG GGCCCCGAGG ACCACCAGGA 
CAACATGGTT CTTCTGGAGT CACAGGACCA CCAGGACCTC CAGGATACTG TGACTCCTCT 
CAGTGCGGCA TGCCTTACAA CGGACAAGGC TACCCAGGCC CCTACCAGCC CGGGTCTGAA 
ACCTACGTAG TGCCCATACC AGCCGCGCCA TCGGAAGATA TCGAGACACA GTCGCCAGGA 
TTGCGCTACC AGCGCGGAAA AAGGTCGCTA TCAAGAAAGG AAGCCGAAAG AATCAAAACA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL12A1A ( XM_009293297.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009291572.1 Danio rerio collagen, type XII, alpha 1a (col12a1a), transcript variant X3, mRNA.

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CloneID	ODa48773
Clone ID Related Accession (Same CDS sequence)	XM_009293297.3 , XM_009293297.2
Accession Version	XM_009293297.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9063bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	collagen alpha-1(XII) chain isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293297.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATCA GGTTGTCTTT GGCCGCTGCT GCGCTCTTGA CTCTAGTACT CAGTTCAGTA 
GAGGCTCAAG TGGAGCCTCC GTCAGACTTA AAATTTAAAA TCCTGAATGA AAACACAGTG 
CAAATGTCAT GGAGGAAGCC CTCGTCTCAG ATACAGGGCT ACAGAATACA GGTCACGTCA 
GATACAGATG ATTCTAAAGA ATTGAGTCTT CCTGCCTCGT CCACAAGCAC ATCAATTACA 
GATCTAACTC CAGATGTAGA CTATTCAGTG TCAATCAATT CATATGATGG TGCTGAAGAA 
AGTATCCCTA TCCTTGGACA AATCACAATT CAATCCAGCA ACACAAGTGG ACCAAGGAGG 
CCACAGTCAG AGGCTGTCAA GTGTTCAGTG AGTGCCGTTG CGGATCTGGT TTTCCTGGTG 
GACGGCTCTT GGAGTGTGGG AAGAGAAAAC TTTAGATTTA TCCGGAGTTT CATTGGGGCC 
ATGGCCGGGG CCTTCGACAT TGAAGAGGAT AAGACCAGAG TGGCTGTGGT TCAGTACAGC 
TCGGACACCA GGACAGAGTT CAGTCTAAAC ACACATTTCA GACGACCCGA TGTCCTGCGA 
GCCATTAAAA ATCTGCCGTA CAAGGGTGGA AATACCATGA CGGGTGATGC AATGGACTAT 
CTGGTGAAAA ACACGTTCAC TCAGGCGGCT GGAGCCAGGA AAGGCTTCCC AAAAGTGGCC 
ATGATCATCA CAGATGGAAA ATCTCAAGAC CCCGTAGAGG AGTACGCCGA ACGGTTGAGG 
AACATCGGAG TGGAGATCTT TGTGCTGGGT ATCAAAGGAG CAGATGAGGA CGAGCTGAAG 
GAGATCGCCT CCAGACCCCA TAGCAAGCAT GTCTATAACG TCCCCAACTT CGACATGATC 
AATCAGGTCC AGAAGGCGCT CATCACTGAA GTTTGCTCTG GCGTGGAGGA GCAGCTAAAT 
TCCCTTGTAA GCGGGGAGGA AGTTGTCGAA CCAGCTTCCA ACCTCAGAGT GACAGATATC 
TCCTCCAAAT CCATGAGAAT TGCGTGGGAT TCATCTCCTG GTGATATCAC AGGCTATAAG 
CTGCAGGTTT TTCCCACTAT TGCGAGCGCC AAAAAGCAGG AGCTCTACAC TGGGCCCACG 
ATGACCTTGG CCAATGTCAG AGACCTGACG CCTGAGATGG AGTATGAGAT CAGTCTGTTT 
GCTCTCAAAG GTCTGACTCC TAGTGTGCCG CAAGTTGTCA CTGAGAAAAC TCAGCCAGTG 
AAAGTATTTA CAGAGTGTTC CCTAGAGGTG GATGTCCAAG CTGACATTGT CCTCCTGGTG 
GATGGGTCAT ATAGTATAGG ACTGCCTAAC TTTGCGAAGG TTAGAGCTTT CCTCGAAGTA 
CTTGTGAATT CTTTTGACAT CGGACCTAAC AAAGTGCAGA TCAGCTTGGT TCAGTACAGC 
AGGGATCCTC ACACTGAGTT CGCCTTGAAT AAATTCGATG ACAATGCAGC CATGGTCAAG 
GCGGTCCGCA CCTTCCCCTA CCGAGGTGGA TCCACCAACA CCGGCAAGGC CATGACATAT 
GTGAGGGAAA AGATCTTTGT TTCGGGCCGT GGCGCTCGTG ATAACGTCCC CCGAGTCATG 
GTGCTTATCA CAGACGGCAA GTCCTCAGAT TCTTTCAAAG ACCCCGCCAA CAAACTGAGG 
GATACAGATG TGGAGATTTT TGCAGTGGGA GTAAAAGATG CCGTACGATC TGAGCTGGAG 
GCTATCGCCA ATGTTCCTGC TGATAACCAT GTGTTTGAGG TGGAGGACTT TGATGCTTTC 
GAGCGGATCT CCAAGGAGCT GACCGCGTCC ATCTGCCTCA GAATTGAGCA GGAGCTCCAT 
AACATCAGGA AAAGAAGTTT GCTTCCCCCA AAGTCACTGA GCTTCTCTGA GGTGACCTCC 
AGGAGTTTCA GGACCTCATG GGTTTCTGAT GGAGAGGATA TCCTGTCTTT TCTGGTGCGC 
TTCAGGCCAG CGGCGGACCT AACTGGAGAC TACATCTCTC TGGTGGTTCC CCATGACATC 
ACCAGTACAA TTCTGCCCAA TCTCACGCCA CTCACCACCT ACGAAGTTAA CGTCATTGCT 
CAGTATGATA AAGGAGACAG CTTTCCTTTG ACGGGCGAGG AGACCACTTT AGAAGAGTTG 
GGTTCTGTTC GTAACCTGAT GGTGAGCGAG GAGGCTGTGG ACCGCTTTCG GGTGTCGTGG 
AGAGCTGCTC CAGGATTAGT GCTGAGGTAC AGACTGCTCT ACGAGCCTGT GAGCGGAGGG 
GATAAACTGG AAGCAGAGAC TGATGGGAAT CAGGTCACCA CTGTGCTCAA TGAACTACTA 
CCGGTCACTA CTTACCGGGT CACCGTTTAC CCTGAGTACG CAAGTGGAAG GGGTCCCTCC 
ATGGACACTG AAGGCACCAC CAAAGAAGTT CGAGGTTCTC CACGCGACTT AAGGGTCTTC 
AATGAGACGG TGTCTAGCAT GAGGGTCTCG TGGCGGGCTG CCCCAGGCAA CGTGCTTCAA 
TATCAGGTGG CCTACAAACC TGACGGTGGG GAGAGGAAAG AAATAAGTGT TAAAGGAGAG 
ACAACAACTG CTGTGCTGAA AAATCTGCTG CCGGACACGG AGTATGAGCT GTTTGTTAGC 
GCACGTTACG TCTCTGGACT TGGCGCACCA CTTATGGGAA CAGGCACCAC GCTGGAAGAA 
CTTGGTTCAC CTTCGAATCT GGTAACTTCA GACGTCACTG AGAACAGTTT TGTAGCATCC 
TGGACCGCCG CCCCTGGAAA CGTCCGTCTT TATCGGGTCA CCTGGAAATC CCTCTTTTCA 
GAGGAAGCTG GCGAGAAGAC TGTCCCCGGG GACGTAACCA CCACTGTTCT GGACGGGCTG 
ACACCAGAGA CCCGCTACAA GGTTTCTGTC TACGCTGTCT ACAGCCGTGC GGAGGGGGCG 
CCACTGCACG GAGAGGAGAC CACAGATGTG TCGGCCGCTA CCAGAGCTCT TACGGTGTCA 
GAAGAAACAG AGAGGACCAT GAAGGTGACA TGGATACCAG CACCTGGCAA TGTTGTCAAT 
TACAGGGTAT CATACAAACC TCAGGGTGAT TTACGCCAAA TGGCCTCCAA AGCACCAGGA 
GGCACCAATT CTATAGTCCT GAGACGCCTG ATCCCCCAGA CCACTTACGA TATCACAGTG 
GTTCCCGTCT ACAAGTTTGG GGATGGAAGA CAAAGGCAAG GAGAAGGAAC TACATTGTCT 
CAATATAAAG CTCCCAGAAA CTTGCAAACT TCGGAGCCCA CTAAAACCAG TTTCAGGGTG 
ACCTGGGATC ATGCTCCTGG AGATGTAAAA GGCTACAAGG TCACTTTCCA TCCCACCGGA 
AAGGAAGTGG ACCTGGGAGA GTTACTCGTA GGCCCCTATG ACAACACTGT GGTTCTGGAG 
GAACTAAGGG CCGGCACTAA GTATACGGTA AATGTGTTTG GTATGTTTGA TGGAGGAGAG 
AGTATGCCAT TGGCTGGAGA GGAGAAGACC ACCCTCTCTG ACGCAGCTTC TGAAAACCTC 
CCTTATACAC CTTCAGATGC CCAGTGTAAA ACCACGGCCC AAGCGGATAT CGTCCTGCTT 
GTTGATGGAT CTTGGAGCAT TGGTCGCCTG AACTTCAAGA CCATACGCAA TTTCATTGCT 
CGCATGGTGG GAGTCTTCGA CATCGGCCCC GATCGTGTCC AGATTGGTCT GGCACAGTAC 
AGCGGAGACC CGAAGACTGA GTGGCATCTC AATGCTCACA GAACCAGGAC TCAGCTGCTG 
GATGCGGTGG CCAATCTACC CTACAAAGGA GGAAACACTC TCACCGGCCT TGCTTTGAAT 
TACATCCTTC AGAATAACTT CAAGCCTAAC GTTGGCATGC GTCCAAACTC CAGAAAGATC 
GGCGTCCTGG TCACTGATGG CAAGTCCCAA GACGACATTG TGGCGAACTC ACAGAACCTG 
CGGGACCAAG GGATTGAACT GTACGCCATT GGTGTAAAGA ACGCAGATGA GAATGAGTTG 
AGAACCATTG CTTCCGATCC CGATGACATT CACATGTACA ACGTGGCTGA TTTCTCCTTC 
CTGCTGGACA TTGTGGATGA CCTCACAAAC AACCTGTGCA ACAGCGTCAA AGGTCCAGGA 
GGAGGACCAG AGCCACCCAC TGATCTGACC ACCTCAGAGG TCACACATTA CTCTTTCCGT 
GCCACTTGGG TGGCTCCTGA CGGGCCTGTG GAGAAGTACA GAGTGGAGTA TGTGAGCACC 
GCTGGAGGAG AACCACAGCA ACAGATGTTT GTGGACGGGA CGGTGACCAC TGTGGTTTTG 
GAAGGTCTCA CTCCGCTGAC CGAGTACATA GTAAAGGTTT ACTCAGTGGT GGGAGAGGAC 
AGCAGCGACC CACTCAGAGG CATAGAGACC ACATTGCCTC TTCCTCCATC CGGTGAGTTG 
AGGATTACCG ATGTGACCCA CAGCACCATG CAGCTGGACT GGGACGCTGC GCCCGGCGCC 
GTCCGAAAGT ACATCATCAC TTACAAGCCA GAGGAGGGAG AGGCGAAGGA GCTTGAAGTT 
GACGGAGATG TAACATCTAG AGGCCTGGAT AATCTCATCT CCCAGACTGA ATATGATGTG 
GCTGTGACTC CGGTTTATGA TTCGGGCACT GGAAACCCCA TGCTTAACCA GGCCATTACA 
GATGTGGTTC CTGCCCCGCA GAACTTGAGG TTCTCGATGG TGACCCAGAC CAGTTTTAGG 
GTTCACTGGG AGCACGGCGC TCCTGATGTC TCCCTCTACC GTATTGGCTG GAAAAAGAAA 
GGAACGACCG AGTTTCAAAA TGATATCCTG AGTAGTGAAG ACACTACATA TGATCTGGTG 
GACCTGGAGC CAGACACACC TTATGATGTT TCGGTCACTG CCATTTACCC TGATGAGTCT 
GAGAGCGAGG ATCTGCTGGG CACTGAACGC ACTTTGGCCA AGCTTGCTAC ACCACCTCCC 
AGTGGTCCTC CTCAAAACCT CCAGGTGTTC GACGAGACCA CCACCTCCCT CACGACTAAG 
TGGGACCACG CGCCTGGACG AGTTCAGAAC TACAAAATCA CCTACGTACC GCTTACTGGA 
GGACGATCAC AGTCGACGCA GGTCGGAGGA AAGAAAAACA CAGTGATCCT GCAGAAACTG 
ACTCCAGACA CTCCTTACTC CATCACTGTT GCTGCGGTCT ACGCCAGCGG AGAGGCCAGA 
GACATTTCTG GCCAAGGAAA GACATTGCCG CTGGGCAGTG TCAGGAATCT TCAGGTCACT 
GACCCCACAG TTAGCACTCT GAATGTGCGC TGGGAGCCGG CGGAGGGAAG CGTTCGCCAG 
TACAGGATCT ACTACCAGCC CGTCGCAGGG GGCGCAGAGG ACATGGTCCA GGTTCCTGGA 
AGCACCACCA GCACCGTTCT GAAGAACCTG CAATCTGACA CTCAGTACAG AGTGACGGTG 
GTGCCAGTCT ACGCTCCTGG AGAGGGCAAG CGCATGTCGG AGACCGGAAA AACACTGGTT 
CGGACCCCTG CCAAGAACAT CCAGGTTTAC AACCCCACCA CAAACACCCT GAACGTGCGC 
TGGGAGCCGG CCTCAGGACA GGTGCAGCAG TACCGCGTGG CTTACGCCCC TCTCTCCGGG 
ATCCGACCCT CTCAGTCGGT GCTGGTTCCA GGAAACACCC ACACGGCTCT GCTGCAGCAG 
CTGGAATCAG ACACTGACTA CTCTGTGAGT GTGGTGGCTC TGTATGCTGA TGGAGAGGGC 
TCGTCTGTGT CTGATAATGG CAAAACACTG CCCCGCAGTG GCCCCAGGAA CCTGCGGGTT 
TATGATCCCA CCACCAGCAC GCTGTCGGTG GGCTGGGAAC ATGCAGAAGG GCCTGTGATT 
CAGTACCGCA TTGGCTACGC ACCCACCACT GGAGATCCCA TTGAAGAATT TACAACTGTC 
CCAGGCAACC GTAACTCGGT CATCCTGCAA AACCTGCAGC CAGACACGCC GTACAACATC 
AACGTAGAAG CCATTTATCA AGATGGCCCT GGAGGACGAC TCAGTGGAGA TGGAAAAACA 
TTGGATCTCC TTGGGCCTCG TAACCTGCGT GTTTCAGACG AGTGGTACAC ACGTTTCCGA 
GTGTCCTGGG ATCCTGCAGC CGCACGGGTG AATGGATATA AACTGGTCTA TACACCAGTG 
GGCACAACCG ACACCATGGA AGCCTTCGTT GGAGACGTGA CTTCACACCA CCTGAACAAC 
CTCCAGCCTG GCACCACATA CGACCTGAAG GTTTACGCTC AGTATGATGC GGGCTTGAGC 
GGCGCTCTGG TCGGACAGGG AACCACCCTG TATCTCAACG TGACCGACCT CACTTCCTAC 
AACGTGGGCT ACGACTCTTT CTGCATCAGA TGGACTCCTC ATCGAGCTGC CACCTCCTAC 
AGACTCAAAG TCAACCCTTT CAACCCATCT CACCGTGGAG CTCAGGAGGT CACGATCACT 
GCGGCTGAGA GCCAGTACTG TTTTGACGGC CTGACGCCAG ACGCGCTATA CAACGCCACC 
GTCTACACGC AGACCCCCAA CCGAGAGGGG CCCGGAGTCA GTGTCAAAGA GCGCACACTG 
GTGAAGCCCA CCGTCGCCCC AACACTACCT CCAACTCCTC CACCTCCAGC CACCATTCCT 
TCTGCACTCG AGGTGTGCAA AGGTGCCAAG GCTGACCTGG TGTTCCTGAT CGATGGCTCG 
TGGAGTATCG GAGATGACAG CTTCGCTAAA GTCCGGCAGT TTGTGTTCAG CATGATTGGG 
GCGTTTGATG TGGTCAGCCA TGAGGGCATG CAGGTGTCTT TCGTGCAGTA CAGCGACGAC 
GCCAAAACTG AGTTCAAGCT CAACACCTAC CACGACAAGG GAATGGTGCT GTCTGCTCTT 
CAGATGGTCA ATTATAGAGG AGGAAACACC AAAACAGGTG CTGCACTGAA GCATGTTGGA 
GAAAAAGTGT TCACCTCGGA CAATGGCATG AGGAGAACCG TTCCCAAGGT ACTGGTTGTG 
GTGACCGATG GACGTTCTCA AGATGACGTG AAGAAGAGCG CTGCTAAACT ACAGCATGCA 
GGTTACAGTG TGTTTGTGGT GGGTGTCGCT GACATTGACG TTAATGAACT GAGGAACATT 
GGAAGCAAGC CCAGTGACAG ACACGTCTTT ATCGTCGACA ACTTCGATGC TTTCGCCAAG 
ATCCAAGACA ACCTGATCAC GTTCATCTGT GAAACGGCAA CGTCCACATG CCCTTTGATT 
TACCTCAATG GATTCACATC ACCAGGCTTC AGGATGCTGG AAGCTTTCAA CATCACTGAC 
AAGACCTACG CAGGCATGAA CGGCGTCTCT CTGGAAACCG GCTCCTTCAA CAGCTTCATC 
GCATACAGAC TCCATAAGGA CGCCTTCATT TCCCAGCCAA CAAAGGAAAT CCATCCAGAT 
GGTCTTCCTC CATCCTACAC CCTCATCCTC CTCTTCCGTC TGCTCCCGGA CACCGGCAAG 
GATGCGTTTG ATTTATGGCA GATCTCAGAT AAGAACAATA ACCCTGAGGT CGGGCTCACC 
ATTGACCCCT CCAGCAGAGT GATCTCCTTC TACAATAAAG ACACTAGAGG GGAAATTCAG 
AAAGTCTCCT TTGATAACGA CCACGTGAAG AAGATTTTCC ACGGCAGTTT CCACAAGCTT 
CACATTTCTA TTTCGCCGAA AAATGTCAAA CTCAACATCG ACTGCCAAGA GGTGGCTGAG 
AAACAGATCA AAGCAGCCAA CAACGTCTCC ACAGATGGCT ACGAAGTCCT TGGAAAAATG 
ACAAAGTCTG GCGGTTCAAA GAAGCAGTCG GCCACATTCC AGCTCCAGAT GTTTGATATT 
GTCTGCAGTT TGGGCTGGAC AAGTCGAGAC AAATGTTGTG ATATTCCAGC AATGCGAGAT 
GAAGCCAAGT GTCCTGCTCT TCCTCACTCC TGCACATGTG CTCAGGACAG TGTTGGTCCT 
CCAGGCCCAG CGGGTCCTCA AGGCAGTCCT GGTACAAAAG GCCCACGAGG CGAGAGAGGA 
GAGTCTGGTC CTACGGGTCC AATGGGTCCA CGTGGAGAGC AGGGTCTGCC AGGGCAGATG 
GGGCCACCGG GTCCACAGGG CCCCAATGGA CTATCCCTTC CCGGAGAACC AGGGCGACCG 
GGACCTAAGG GAGACCCCGG TGACTCAGGT TTACCTGGAC AGAGAGGCCC GGTTGGCGCT 
GCAGGTCCAC TCGGCCCTGT TGGACCCGCT GGAGCAAGAG GTCCACCAGG AAGTGAAGGC 
CCATCTGGAC CAAGAGGTCC ATCGGGACCA ATGGGACCAC CGGGAGCTCC CGGAATGCCA 
GGAATCACCG GAAAACCAGG AAAACCCGGA GACACTGGCT TTCCTGGACC TGTTGGCATG 
AAAGGAGAGA AGGGTGAAAG AGGAGACACT GCGTCACAGA ATATGATGCG CTCTGTAGCG 
AGACAAGTCT GCGAACAGCT AGTCAGCAGA CAAATGAGCA GAATTGATAT GATGATGAAC 
CAGATCCCCA GCAACTACAG GAGTAACAGC CCCGGCCCCG CTGGACCTCC AGGCCCACCT 
GGAAACCAGG GACCCCATGG AGAGCCTGGT CAGCCCGGAC CCAATGGCTT CCCAGGAAAT 
CCAGGTTTAC CAGGCTCTCC TGGAGAAAGA GGAGTGCCTG GAGAGAAGGG TGAACGAGGC 
AGCCCCGGCA TTGGCACTCG AGGACAGAGA GGATTACCTG GACCTCCTGG GCCACCAGGG 
CAGTCTCAGA CCGGTCCCCC TGGTCCCTCA GGCTCACCCG GGCCCCGAGG ACCACCAGGA 
CAACATGGTT CTTCTGGAGT CACAGGACCA CCAGGACCTC CAGGATACTG TGACTCCTCT 
CAGTGCGGCA TGCCTTACAA CGGACAAGGC TACCCAGGCC CCTACCAGCC CGGGTCTGAA 
ACCTACGTAG TGCCCATACC AGCCGCGCCA TCGGAAGATA TCGAGACACA GTCGCCAGGA 
TTGCGCTACC AGCGCGGAAA AAGGTCGCTA TCAAGAAAGG AAGCCGAAAG AATCAAAACA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291572.1
CDS	140..9202
Translation	MKIRLSLAAAALLTLVLSSVEAQVEPPSDLKFKILNENTVQMSWRKPSSQIQGYRIQVTSDTDDSKELSLPASSTSTSITDLTPDVDYSVSINSYDGAEESIPILGQITIQSSNTSGPRRPQSEAVKCSVSAVADLVFLVDGSWSVGRENFRFIRSFIGAMAGAFDIEEDKTRVAVVQYSSDTRTEFSLNTHFRRPDVLRAIKNLPYKGGNTMTGDAMDYLVKNTFTQAAGARKGFPKVAMIITDGKSQDPVEEYAERLRNIGVEIFVLGIKGADEDELKEIASRPHSKHVYNVPNFDMINQVQKALITEVCSGVEEQLNSLVSGEEVVEPASNLRVTDISSKSMRIAWDSSPGDITGYKLQVFPTIASAKKQELYTGPTMTLANVRDLTPEMEYEISLFALKGLTPSVPQVVTEKTQPVKVFTECSLEVDVQADIVLLVDGSYSIGLPNFAKVRAFLEVLVNSFDIGPNKVQISLVQYSRDPHTEFALNKFDDNAAMVKAVRTFPYRGGSTNTGKAMTYVREKIFVSGRGARDNVPRVMVLITDGKSSDSFKDPANKLRDTDVEIFAVGVKDAVRSELEAIANVPADNHVFEVEDFDAFERISKELTASICLRIEQELHNIRKRSLLPPKSLSFSEVTSRSFRTSWVSDGEDILSFLVRFRPAADLTGDYISLVVPHDITSTILPNLTPLTTYEVNVIAQYDKGDSFPLTGEETTLEELGSVRNLMVSEEAVDRFRVSWRAAPGLVLRYRLLYEPVSGGDKLEAETDGNQVTTVLNELLPVTTYRVTVYPEYASGRGPSMDTEGTTKEVRGSPRDLRVFNETVSSMRVSWRAAPGNVLQYQVAYKPDGGERKEISVKGETTTAVLKNLLPDTEYELFVSARYVSGLGAPLMGTGTTLEELGSPSNLVTSDVTENSFVASWTAAPGNVRLYRVTWKSLFSEEAGEKTVPGDVTTTVLDGLTPETRYKVSVYAVYSRAEGAPLHGEETTDVSAATRALTVSEETERTMKVTWIPAPGNVVNYRVSYKPQGDLRQMASKAPGGTNSIVLRRLIPQTTYDITVVPVYKFGDGRQRQGEGTTLSQYKAPRNLQTSEPTKTSFRVTWDHAPGDVKGYKVTFHPTGKEVDLGELLVGPYDNTVVLEELRAGTKYTVNVFGMFDGGESMPLAGEEKTTLSDAASENLPYTPSDAQCKTTAQADIVLLVDGSWSIGRLNFKTIRNFIARMVGVFDIGPDRVQIGLAQYSGDPKTEWHLNAHRTRTQLLDAVANLPYKGGNTLTGLALNYILQNNFKPNVGMRPNSRKIGVLVTDGKSQDDIVANSQNLRDQGIELYAIGVKNADENELRTIASDPDDIHMYNVADFSFLLDIVDDLTNNLCNSVKGPGGGPEPPTDLTTSEVTHYSFRATWVAPDGPVEKYRVEYVSTAGGEPQQQMFVDGTVTTVVLEGLTPLTEYIVKVYSVVGEDSSDPLRGIETTLPLPPSGELRITDVTHSTMQLDWDAAPGAVRKYIITYKPEEGEAKELEVDGDVTSRGLDNLISQTEYDVAVTPVYDSGTGNPMLNQAITDVVPAPQNLRFSMVTQTSFRVHWEHGAPDVSLYRIGWKKKGTTEFQNDILSSEDTTYDLVDLEPDTPYDVSVTAIYPDESESEDLLGTERTLAKLATPPPSGPPQNLQVFDETTTSLTTKWDHAPGRVQNYKITYVPLTGGRSQSTQVGGKKNTVILQKLTPDTPYSITVAAVYASGEARDISGQGKTLPLGSVRNLQVTDPTVSTLNVRWEPAEGSVRQYRIYYQPVAGGAEDMVQVPGSTTSTVLKNLQSDTQYRVTVVPVYAPGEGKRMSETGKTLVRTPAKNIQVYNPTTNTLNVRWEPASGQVQQYRVAYAPLSGIRPSQSVLVPGNTHTALLQQLESDTDYSVSVVALYADGEGSSVSDNGKTLPRSGPRNLRVYDPTTSTLSVGWEHAEGPVIQYRIGYAPTTGDPIEEFTTVPGNRNSVILQNLQPDTPYNINVEAIYQDGPGGRLSGDGKTLDLLGPRNLRVSDEWYTRFRVSWDPAAARVNGYKLVYTPVGTTDTMEAFVGDVTSHHLNNLQPGTTYDLKVYAQYDAGLSGALVGQGTTLYLNVTDLTSYNVGYDSFCIRWTPHRAATSYRLKVNPFNPSHRGAQEVTITAAESQYCFDGLTPDALYNATVYTQTPNREGPGVSVKERTLVKPTVAPTLPPTPPPPATIPSALEVCKGAKADLVFLIDGSWSIGDDSFAKVRQFVFSMIGAFDVVSHEGMQVSFVQYSDDAKTEFKLNTYHDKGMVLSALQMVNYRGGNTKTGAALKHVGEKVFTSDNGMRRTVPKVLVVVTDGRSQDDVKKSAAKLQHAGYSVFVVGVADIDVNELRNIGSKPSDRHVFIVDNFDAFAKIQDNLITFICETATSTCPLIYLNGFTSPGFRMLEAFNITDKTYAGMNGVSLETGSFNSFIAYRLHKDAFISQPTKEIHPDGLPPSYTLILLFRLLPDTGKDAFDLWQISDKNNNPEVGLTIDPSSRVISFYNKDTRGEIQKVSFDNDHVKKIFHGSFHKLHISISPKNVKLNIDCQEVAEKQIKAANNVSTDGYEVLGKMTKSGGSKKQSATFQLQMFDIVCSLGWTSRDKCCDIPAMRDEAKCPALPHSCTCAQDSVGPPGPAGPQGSPGTKGPRGERGESGPTGPMGPRGEQGLPGQMGPPGPQGPNGLSLPGEPGRPGPKGDPGDSGLPGQRGPVGAAGPLGPVGPAGARGPPGSEGPSGPRGPSGPMGPPGAPGMPGITGKPGKPGDTGFPGPVGMKGEKGERGDTASQNMMRSVARQVCEQLVSRQMSRIDMMMNQIPSNYRSNSPGPAGPPGPPGNQGPHGEPGQPGPNGFPGNPGLPGSPGERGVPGEKGERGSPGIGTRGQRGLPGPPGPPGQSQTGPPGPSGSPGPRGPPGQHGSSGVTGPPGPPGYCDSSQCGMPYNGQGYPGPYQPGSETYVVPIPAAPSEDIETQSPGLRYQRGKRSLSRKEAERIKT

Target ORF information:

RefSeq Version	XM_009293297.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio collagen, type XII, alpha 1a (col12a1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293297.2

ORF Insert Sequence:

ATGAAGATCA GGTTGTCTTT GGCCGCTGCT GCGCTCTTGA CTCTAGTACT CAGTTCAGTA 
GAGGCTCAAG TGGAGCCTCC GTCAGACTTA AAATTTAAAA TCCTGAATGA AAACACAGTG 
CAAATGTCAT GGAGGAAGCC CTCGTCTCAG ATACAGGGCT ACAGAATACA GGTCACGTCA 
GATACAGATG ATTCTAAAGA ATTGAGTCTT CCTGCCTCGT CCACAAGCAC ATCAATTACA 
GATCTAACTC CAGATGTAGA CTATTCAGTG TCAATCAATT CATATGATGG TGCTGAAGAA 
AGTATCCCTA TCCTTGGACA AATCACAATT CAATCCAGCA ACACAAGTGG ACCAAGGAGG 
CCACAGTCAG AGGCTGTCAA GTGTTCAGTG AGTGCCGTTG CGGATCTGGT TTTCCTGGTG 
GACGGCTCTT GGAGTGTGGG AAGAGAAAAC TTTAGATTTA TCCGGAGTTT CATTGGGGCC 
ATGGCCGGGG CCTTCGACAT TGAAGAGGAT AAGACCAGAG TGGCTGTGGT TCAGTACAGC 
TCGGACACCA GGACAGAGTT CAGTCTAAAC ACACATTTCA GACGACCCGA TGTCCTGCGA 
GCCATTAAAA ATCTGCCGTA CAAGGGTGGA AATACCATGA CGGGTGATGC AATGGACTAT 
CTGGTGAAAA ACACGTTCAC TCAGGCGGCT GGAGCCAGGA AAGGCTTCCC AAAAGTGGCC 
ATGATCATCA CAGATGGAAA ATCTCAAGAC CCCGTAGAGG AGTACGCCGA ACGGTTGAGG 
AACATCGGAG TGGAGATCTT TGTGCTGGGT ATCAAAGGAG CAGATGAGGA CGAGCTGAAG 
GAGATCGCCT CCAGACCCCA TAGCAAGCAT GTCTATAACG TCCCCAACTT CGACATGATC 
AATCAGGTCC AGAAGGCGCT CATCACTGAA GTTTGCTCTG GCGTGGAGGA GCAGCTAAAT 
TCCCTTGTAA GCGGGGAGGA AGTTGTCGAA CCAGCTTCCA ACCTCAGAGT GACAGATATC 
TCCTCCAAAT CCATGAGAAT TGCGTGGGAT TCATCTCCTG GTGATATCAC AGGCTATAAG 
CTGCAGGTTT TTCCCACTAT TGCGAGCGCC AAAAAGCAGG AGCTCTACAC TGGGCCCACG 
ATGACCTTGG CCAATGTCAG AGACCTGACG CCTGAGATGG AGTATGAGAT CAGTCTGTTT 
GCTCTCAAAG GTCTGACTCC TAGTGTGCCG CAAGTTGTCA CTGAGAAAAC TCAGCCAGTG 
AAAGTATTTA CAGAGTGTTC CCTAGAGGTG GATGTCCAAG CTGACATTGT CCTCCTGGTG 
GATGGGTCAT ATAGTATAGG ACTGCCTAAC TTTGCGAAGG TTAGAGCTTT CCTCGAAGTA 
CTTGTGAATT CTTTTGACAT CGGACCTAAC AAAGTGCAGA TCAGCTTGGT TCAGTACAGC 
AGGGATCCTC ACACTGAGTT CGCCTTGAAT AAATTCGATG ACAATGCAGC CATGGTCAAG 
GCGGTCCGCA CCTTCCCCTA CCGAGGTGGA TCCACCAACA CCGGCAAGGC CATGACATAT 
GTGAGGGAAA AGATCTTTGT TTCGGGCCGT GGCGCTCGTG ATAACGTCCC CCGAGTCATG 
GTGCTTATCA CAGACGGCAA GTCCTCAGAT TCTTTCAAAG ACCCCGCCAA CAAACTGAGG 
GATACAGATG TGGAGATTTT TGCAGTGGGA GTAAAAGATG CCGTACGATC TGAGCTGGAG 
GCTATCGCCA ATGTTCCTGC TGATAACCAT GTGTTTGAGG TGGAGGACTT TGATGCTTTC 
GAGCGGATCT CCAAGGAGCT GACCGCGTCC ATCTGCCTCA GAATTGAGCA GGAGCTCCAT 
AACATCAGGA AAAGAAGTTT GCTTCCCCCA AAGTCACTGA GCTTCTCTGA GGTGACCTCC 
AGGAGTTTCA GGACCTCATG GGTTTCTGAT GGAGAGGATA TCCTGTCTTT TCTGGTGCGC 
TTCAGGCCAG CGGCGGACCT AACTGGAGAC TACATCTCTC TGGTGGTTCC CCATGACATC 
ACCAGTACAA TTCTGCCCAA TCTCACGCCA CTCACCACCT ACGAAGTTAA CGTCATTGCT 
CAGTATGATA AAGGAGACAG CTTTCCTTTG ACGGGCGAGG AGACCACTTT AGAAGAGTTG 
GGTTCTGTTC GTAACCTGAT GGTGAGCGAG GAGGCTGTGG ACCGCTTTCG GGTGTCGTGG 
AGAGCTGCTC CAGGATTAGT GCTGAGGTAC AGACTGCTCT ACGAGCCTGT GAGCGGAGGG 
GATAAACTGG AAGCAGAGAC TGATGGGAAT CAGGTCACCA CTGTGCTCAA TGAACTACTA 
CCGGTCACTA CTTACCGGGT CACCGTTTAC CCTGAGTACG CAAGTGGAAG GGGTCCCTCC 
ATGGACACTG AAGGCACCAC CAAAGAAGTT CGAGGTTCTC CACGCGACTT AAGGGTCTTC 
AATGAGACGG TGTCTAGCAT GAGGGTCTCG TGGCGGGCTG CCCCAGGCAA CGTGCTTCAA 
TATCAGGTGG CCTACAAACC TGACGGTGGG GAGAGGAAAG AAATAAGTGT TAAAGGAGAG 
ACAACAACTG CTGTGCTGAA AAATCTGCTG CCGGACACGG AGTATGAGCT GTTTGTTAGC 
GCACGTTACG TCTCTGGACT TGGCGCACCA CTTATGGGAA CAGGCACCAC GCTGGAAGAA 
CTTGGTTCAC CTTCGAATCT GGTAACTTCA GACGTCACTG AGAACAGTTT TGTAGCATCC 
TGGACCGCCG CCCCTGGAAA CGTCCGTCTT TATCGGGTCA CCTGGAAATC CCTCTTTTCA 
GAGGAAGCTG GCGAGAAGAC TGTCCCCGGG GACGTAACCA CCACTGTTCT GGACGGGCTG 
ACACCAGAGA CCCGCTACAA GGTTTCTGTC TACGCTGTCT ACAGCCGTGC GGAGGGGGCG 
CCACTGCACG GAGAGGAGAC CACAGATGTG TCGGCCGCTA CCAGAGCTCT TACGGTGTCA 
GAAGAAACAG AGAGGACCAT GAAGGTGACA TGGATACCAG CACCTGGCAA TGTTGTCAAT 
TACAGGGTAT CATACAAACC TCAGGGTGAT TTACGCCAAA TGGCCTCCAA AGCACCAGGA 
GGCACCAATT CTATAGTCCT GAGACGCCTG ATCCCCCAGA CCACTTACGA TATCACAGTG 
GTTCCCGTCT ACAAGTTTGG GGATGGAAGA CAAAGGCAAG GAGAAGGAAC TACATTGTCT 
CAATATAAAG CTCCCAGAAA CTTGCAAACT TCGGAGCCCA CTAAAACCAG TTTCAGGGTG 
ACCTGGGATC ATGCTCCTGG AGATGTAAAA GGCTACAAGG TCACTTTCCA TCCCACCGGA 
AAGGAAGTGG ACCTGGGAGA GTTACTCGTA GGCCCCTATG ACAACACTGT GGTTCTGGAG 
GAACTAAGGG CCGGCACTAA GTATACGGTA AATGTGTTTG GTATGTTTGA TGGAGGAGAG 
AGTATGCCAT TGGCTGGAGA GGAGAAGACC ACCCTCTCTG ACGCAGCTTC TGAAAACCTC 
CCTTATACAC CTTCAGATGC CCAGTGTAAA ACCACGGCCC AAGCGGATAT CGTCCTGCTT 
GTTGATGGAT CTTGGAGCAT TGGTCGCCTG AACTTCAAGA CCATACGCAA TTTCATTGCT 
CGCATGGTGG GAGTCTTCGA CATCGGCCCC GATCGTGTCC AGATTGGTCT GGCACAGTAC 
AGCGGAGACC CGAAGACTGA GTGGCATCTC AATGCTCACA GAACCAGGAC TCAGCTGCTG 
GATGCGGTGG CCAATCTACC CTACAAAGGA GGAAACACTC TCACCGGCCT TGCTTTGAAT 
TACATCCTTC AGAATAACTT CAAGCCTAAC GTTGGCATGC GTCCAAACTC CAGAAAGATC 
GGCGTCCTGG TCACTGATGG CAAGTCCCAA GACGACATTG TGGCGAACTC ACAGAACCTG 
CGGGACCAAG GGATTGAACT GTACGCCATT GGTGTAAAGA ACGCAGATGA GAATGAGTTG 
AGAACCATTG CTTCCGATCC CGATGACATT CACATGTACA ACGTGGCTGA TTTCTCCTTC 
CTGCTGGACA TTGTGGATGA CCTCACAAAC AACCTGTGCA ACAGCGTCAA AGGTCCAGGA 
GGAGGACCAG AGCCACCCAC TGATCTGACC ACCTCAGAGG TCACACATTA CTCTTTCCGT 
GCCACTTGGG TGGCTCCTGA CGGGCCTGTG GAGAAGTACA GAGTGGAGTA TGTGAGCACC 
GCTGGAGGAG AACCACAGCA ACAGATGTTT GTGGACGGGA CGGTGACCAC TGTGGTTTTG 
GAAGGTCTCA CTCCGCTGAC CGAGTACATA GTAAAGGTTT ACTCAGTGGT GGGAGAGGAC 
AGCAGCGACC CACTCAGAGG CATAGAGACC ACATTGCCTC TTCCTCCATC CGGTGAGTTG 
AGGATTACCG ATGTGACCCA CAGCACCATG CAGCTGGACT GGGACGCTGC GCCCGGCGCC 
GTCCGAAAGT ACATCATCAC TTACAAGCCA GAGGAGGGAG AGGCGAAGGA GCTTGAAGTT 
GACGGAGATG TAACATCTAG AGGCCTGGAT AATCTCATCT CCCAGACTGA ATATGATGTG 
GCTGTGACTC CGGTTTATGA TTCGGGCACT GGAAACCCCA TGCTTAACCA GGCCATTACA 
GATGTGGTTC CTGCCCCGCA GAACTTGAGG TTCTCGATGG TGACCCAGAC CAGTTTTAGG 
GTTCACTGGG AGCACGGCGC TCCTGATGTC TCCCTCTACC GTATTGGCTG GAAAAAGAAA 
GGAACGACCG AGTTTCAAAA TGATATCCTG AGTAGTGAAG ACACTACATA TGATCTGGTG 
GACCTGGAGC CAGACACACC TTATGATGTT TCGGTCACTG CCATTTACCC TGATGAGTCT 
GAGAGCGAGG ATCTGCTGGG CACTGAACGC ACTTTGGCCA AGCTTGCTAC ACCACCTCCC 
AGTGGTCCTC CTCAAAACCT CCAGGTGTTC GACGAGACCA CCACCTCCCT CACGACTAAG 
TGGGACCACG CGCCTGGACG AGTTCAGAAC TACAAAATCA CCTACGTACC GCTTACTGGA 
GGACGATCAC AGTCGACGCA GGTCGGAGGA AAGAAAAACA CAGTGATCCT GCAGAAACTG 
ACTCCAGACA CTCCTTACTC CATCACTGTT GCTGCGGTCT ACGCCAGCGG AGAGGCCAGA 
GACATTTCTG GCCAAGGAAA GACATTGCCG CTGGGCAGTG TCAGGAATCT TCAGGTCACT 
GACCCCACAG TTAGCACTCT GAATGTGCGC TGGGAGCCGG CGGAGGGAAG CGTTCGCCAG 
TACAGGATCT ACTACCAGCC CGTCGCAGGG GGCGCAGAGG ACATGGTCCA GGTTCCTGGA 
AGCACCACCA GCACCGTTCT GAAGAACCTG CAATCTGACA CTCAGTACAG AGTGACGGTG 
GTGCCAGTCT ACGCTCCTGG AGAGGGCAAG CGCATGTCGG AGACCGGAAA AACACTGGTT 
CGGACCCCTG CCAAGAACAT CCAGGTTTAC AACCCCACCA CAAACACCCT GAACGTGCGC 
TGGGAGCCGG CCTCAGGACA GGTGCAGCAG TACCGCGTGG CTTACGCCCC TCTCTCCGGG 
ATCCGACCCT CTCAGTCGGT GCTGGTTCCA GGAAACACCC ACACGGCTCT GCTGCAGCAG 
CTGGAATCAG ACACTGACTA CTCTGTGAGT GTGGTGGCTC TGTATGCTGA TGGAGAGGGC 
TCGTCTGTGT CTGATAATGG CAAAACACTG CCCCGCAGTG GCCCCAGGAA CCTGCGGGTT 
TATGATCCCA CCACCAGCAC GCTGTCGGTG GGCTGGGAAC ATGCAGAAGG GCCTGTGATT 
CAGTACCGCA TTGGCTACGC ACCCACCACT GGAGATCCCA TTGAAGAATT TACAACTGTC 
CCAGGCAACC GTAACTCGGT CATCCTGCAA AACCTGCAGC CAGACACGCC GTACAACATC 
AACGTAGAAG CCATTTATCA AGATGGCCCT GGAGGACGAC TCAGTGGAGA TGGAAAAACA 
TTGGATCTCC TTGGGCCTCG TAACCTGCGT GTTTCAGACG AGTGGTACAC ACGTTTCCGA 
GTGTCCTGGG ATCCTGCAGC CGCACGGGTG AATGGATATA AACTGGTCTA TACACCAGTG 
GGCACAACCG ACACCATGGA AGCCTTCGTT GGAGACGTGA CTTCACACCA CCTGAACAAC 
CTCCAGCCTG GCACCACATA CGACCTGAAG GTTTACGCTC AGTATGATGC GGGCTTGAGC 
GGCGCTCTGG TCGGACAGGG AACCACCCTG TATCTCAACG TGACCGACCT CACTTCCTAC 
AACGTGGGCT ACGACTCTTT CTGCATCAGA TGGACTCCTC ATCGAGCTGC CACCTCCTAC 
AGACTCAAAG TCAACCCTTT CAACCCATCT CACCGTGGAG CTCAGGAGGT CACGATCACT 
GCGGCTGAGA GCCAGTACTG TTTTGACGGC CTGACGCCAG ACGCGCTATA CAACGCCACC 
GTCTACACGC AGACCCCCAA CCGAGAGGGG CCCGGAGTCA GTGTCAAAGA GCGCACACTG 
GTGAAGCCCA CCGTCGCCCC AACACTACCT CCAACTCCTC CACCTCCAGC CACCATTCCT 
TCTGCACTCG AGGTGTGCAA AGGTGCCAAG GCTGACCTGG TGTTCCTGAT CGATGGCTCG 
TGGAGTATCG GAGATGACAG CTTCGCTAAA GTCCGGCAGT TTGTGTTCAG CATGATTGGG 
GCGTTTGATG TGGTCAGCCA TGAGGGCATG CAGGTGTCTT TCGTGCAGTA CAGCGACGAC 
GCCAAAACTG AGTTCAAGCT CAACACCTAC CACGACAAGG GAATGGTGCT GTCTGCTCTT 
CAGATGGTCA ATTATAGAGG AGGAAACACC AAAACAGGTG CTGCACTGAA GCATGTTGGA 
GAAAAAGTGT TCACCTCGGA CAATGGCATG AGGAGAACCG TTCCCAAGGT ACTGGTTGTG 
GTGACCGATG GACGTTCTCA AGATGACGTG AAGAAGAGCG CTGCTAAACT ACAGCATGCA 
GGTTACAGTG TGTTTGTGGT GGGTGTCGCT GACATTGACG TTAATGAACT GAGGAACATT 
GGAAGCAAGC CCAGTGACAG ACACGTCTTT ATCGTCGACA ACTTCGATGC TTTCGCCAAG 
ATCCAAGACA ACCTGATCAC GTTCATCTGT GAAACGGCAA CGTCCACATG CCCTTTGATT 
TACCTCAATG GATTCACATC ACCAGGCTTC AGGATGCTGG AAGCTTTCAA CATCACTGAC 
AAGACCTACG CAGGCATGAA CGGCGTCTCT CTGGAAACCG GCTCCTTCAA CAGCTTCATC 
GCATACAGAC TCCATAAGGA CGCCTTCATT TCCCAGCCAA CAAAGGAAAT CCATCCAGAT 
GGTCTTCCTC CATCCTACAC CCTCATCCTC CTCTTCCGTC TGCTCCCGGA CACCGGCAAG 
GATGCGTTTG ATTTATGGCA GATCTCAGAT AAGAACAATA ACCCTGAGGT CGGGCTCACC 
ATTGACCCCT CCAGCAGAGT GATCTCCTTC TACAATAAAG ACACTAGAGG GGAAATTCAG 
AAAGTCTCCT TTGATAACGA CCACGTGAAG AAGATTTTCC ACGGCAGTTT CCACAAGCTT 
CACATTTCTA TTTCGCCGAA AAATGTCAAA CTCAACATCG ACTGCCAAGA GGTGGCTGAG 
AAACAGATCA AAGCAGCCAA CAACGTCTCC ACAGATGGCT ACGAAGTCCT TGGAAAAATG 
ACAAAGTCTG GCGGTTCAAA GAAGCAGTCG GCCACATTCC AGCTCCAGAT GTTTGATATT 
GTCTGCAGTT TGGGCTGGAC AAGTCGAGAC AAATGTTGTG ATATTCCAGC AATGCGAGAT 
GAAGCCAAGT GTCCTGCTCT TCCTCACTCC TGCACATGTG CTCAGGACAG TGTTGGTCCT 
CCAGGCCCAG CGGGTCCTCA AGGCAGTCCT GGTACAAAAG GCCCACGAGG CGAGAGAGGA 
GAGTCTGGTC CTACGGGTCC AATGGGTCCA CGTGGAGAGC AGGGTCTGCC AGGGCAGATG 
GGGCCACCGG GTCCACAGGG CCCCAATGGA CTATCCCTTC CCGGAGAACC AGGGCGACCG 
GGACCTAAGG GAGACCCCGG TGACTCAGGT TTACCTGGAC AGAGAGGCCC GGTTGGCGCT 
GCAGGTCCAC TCGGCCCTGT TGGACCCGCT GGAGCAAGAG GTCCACCAGG AAGTGAAGGC 
CCATCTGGAC CAAGAGGTCC ATCGGGACCA ATGGGACCAC CGGGAGCTCC CGGAATGCCA 
GGAATCACCG GAAAACCAGG AAAACCCGGA GACACTGGCT TTCCTGGACC TGTTGGCATG 
AAAGGAGAGA AGGGTGAAAG AGGAGACACT GCGTCACAGA ATATGATGCG CTCTGTAGCG 
AGACAAGTCT GCGAACAGCT AGTCAGCAGA CAAATGAGCA GAATTGATAT GATGATGAAC 
CAGATCCCCA GCAACTACAG GAGTAACAGC CCCGGCCCCG CTGGACCTCC AGGCCCACCT 
GGAAACCAGG GACCCCATGG AGAGCCTGGT CAGCCCGGAC CCAATGGCTT CCCAGGAAAT 
CCAGGTTTAC CAGGCTCTCC TGGAGAAAGA GGAGTGCCTG GAGAGAAGGG TGAACGAGGC 
AGCCCCGGCA TTGGCACTCG AGGACAGAGA GGATTACCTG GACCTCCTGG GCCACCAGGG 
CAGTCTCAGA CCGGTCCCCC TGGTCCCTCA GGCTCACCCG GGCCCCGAGG ACCACCAGGA 
CAACATGGTT CTTCTGGAGT CACAGGACCA CCAGGACCTC CAGGATACTG TGACTCCTCT 
CAGTGCGGCA TGCCTTACAA CGGACAAGGC TACCCAGGCC CCTACCAGCC CGGGTCTGAA 
ACCTACGTAG TGCCCATACC AGCCGCGCCA TCGGAAGATA TCGAGACACA GTCGCCAGGA 
TTGCGCTACC AGCGCGGAAA AAGGTCGCTA TCAAGAAAGG AAGCCGAAAG AATCAAAACA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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COL12A1A cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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