clstn2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	clstn2
Entrez Gene ID	100125749
Full Name	calsyntenin 2
Synonyms	si:dkeyp-55c8.1
General protein information	Preferred Names calsyntenin 2 Names calsyntenin-2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001159839.1	NP_001153311.1	calsyntenin-2 precursor
XM_009298708.2	XP_009296983.1	calsyntenin-2 isoform X1
XM_009298708.3	XP_009296983.1	calsyntenin-2 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CLSTN2	XP_526327.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007103	XP_001237120.2
Macaca mulatta (Rhesus monkey)	CLSTN2	XP_001113205.1
Bos taurus (cattle)	CLSTN2	NP_001193686.1
Mus musculus (house mouse)	Clstn2	NP_071714.2
Drosophila melanogaster (fruit fly)	Cals	NP_726646.1
Homo sapiens (human)	CLSTN2	NP_071414.2
Canis lupus familiaris (dog)	CLSTN2	XP_534287.3
Rattus norvegicus (Norway rat)	Clstn2	NP_599204.1
Xenopus tropicalis (tropical clawed frog)	clstn2	XP_002941239.2
Gallus gallus (chicken)	CLSTN2	XP_422633.4
Danio rerio (zebrafish)	clstn2	NP_001153311.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequential, Divergent, and Cooperative Requirements of
Yang YJ, Wang Y, Li Z, Zhou L, Gui JF
Genetics205(4)1551-1572(2017 04)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Zebrafish calsyntenins mediate homophilic adhesion through their amino-terminal cadherin repeats.
Ortiz-Medina H, Emond MR, Jontes JD
Neuroscience28687-96(2015 Feb)

Identification of zygotic genes expressed at the midblastula transition in zebrafish.
O'Boyle S, Bree RT, McLoughlin S, Grealy M, Byrnes L
Biochemical and biophysical research communications358(2)462-8(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following clstn2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the clstn2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa34134	NM_001159839.1 Latest version!	Danio rerio calsyntenin 2 (clstn2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa48468	XM_009298708.3 Latest version!	Danio rerio calsyntenin 2 (clstn2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
ODa48468	XM_009298708.2	Danio rerio calsyntenin 2 (clstn2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

clstn2 ( NM_001159839.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001153311.1 Danio rerio calsyntenin 2 (clstn2), mRNA.

Price & Availability↑

CloneID	ODa34134
Clone ID Related Accession (Same CDS sequence)	NM_001159839.1
Accession Version	NM_001159839.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2814bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1542902400000
Organism	Danio rerio(zebrafish)
Product	calsyntenin-2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CU179756.8, BX248328.6, CR936405.6 and BX322588.8. On May 8, 2009 this sequence version replaced XM_001919357.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01765705 [ECO:0000348] ##Evidence-Data-END##

ATGAAGATGC GCGCGATAAC AGCGATGCTT CTCCTGGTGC TCTCCGGGCA GTGCGGCATC 
CTGGCTGGGA AAGTGAATAA GCACAAACCA TGGATTGAGA CATCCTACCA TGGAGTCATT 
ACTGAGAACA TGGACACTGT AATGCTGGAC CCTCCTCTTG TGGCGCTGGA CAAAGACGCT 
CCTGTTCCTT ATGCAGGAGA GATCTGTGCG TTTAAGATCC ATGGGCAGGA GGCGCCGTTT 
GAGGCTGAGG TGTTGAACAG GACATCAGGT GAAGGCGTGC TGAGGGCCCG GGGCCCTATA 
GACTGTGAGC AGCAGAAGGA GTACACCTTC ATCATCCAAG CCTATGACTG TGGGGCCAGT 
CCAAATGGAG CTGACTGGAA AAAGAGTCAC AAGGCGGTGG TTCATATCCA GGTGGATGAT 
GTAAACGAGT TCTCCCCAGT GTTCAGAGAG CCGTTGTATC GCGCCACTGT AACTGAAGGG 
AAGATCTATG ACAGCATCCT GCAGGTGGAG GCCTGGGATC AGGACTGCTC TCCACAGTAC 
AGCCAGATCT GCAACTATGA GATTGTCACA CAGGATACAC CCTTCGCCAT TGATCGAAAT 
GGCAACATAA GAAACACAGA GCGACTGAGC TTCGACAAAC AACAGCACTA TAAAATCATG 
GTGACGGCCT ACGATTGTGG CCAGAAGAGG GCGATGGAGA GCGTTCCAGT TCACATTGAC 
GTCAAACCAG TGTGCAAGCC AGGCTGGCAA GGTTGGAACA AGCGTGTAGA TTATGAACCT 
GGAACAGGCA GCAAGCAGCT CTTTCCCAAG ATGCACCTGG AGACCTGCGA TGGCCCCTTA 
TCCTCAGTGC GAGCAATGGT GGAGCTACAG ACCAGCCATA TTGGAAAGGG CTGCGACAGA 
GAGACCTATT CCGAGAAGTC TCTGCAGAAG CTTTGCGGAG CAGCCTCGGG CAGCACAGAC 
CTTCTCCCGG CTCCTAGCAC ATCCACCAAT TGGACGGCAT CCCTGCTGAC AGACAGTGGC 
CGAGACAGTG ACCTCATCTT CCGATTTGAT GGACGACAGG CAGCCAACAT CCCAGATTGG 
GTTGTGCCAC AGAACCTGAC AGATCAGTTC ACCATAGCAA CATGGATGAA GCATGGGCCG 
AGTCCTGGAC TGAGAGCTGA GAAGGAGACG TTGCTCTGTA ATTCAGATAA AACTGAGATG 
AACAGACATC ATTATTCTCT GTATGTCCAC AACTGCCGTC TGGTGTTTCT TCTGAGGCGC 
GACTTCATTC AGCTGGACTC CTTCAGACCG GCCGAATTTC ACTGGAGACT GGAACAGATC 
TGTGATAAAG AGTGGCATTA CTACGTCATC AATGTGGAGT TTCCATCAGT AACCCTCTTC 
GTAGACGGAG TGACATACGA ACCATACCTG GTGACAGACG ACTGGCCCAT CCACCCTTCA 
GAGATCGATG TACAGCTGAC CGTCGGCGCC TGCTGGCAAG GAGGAGAAGT GACCACTCCA 
AGGTTTACAC AGTATTTCCG AGGAAGCCTC TCGGGGTTGA CTATCCGACC AGGGAAAATC 
GCCACTCAGA AGGTGATCTC ATGCCTACAG GCTTGCAAGG AAGGTCTCGA CATCAGCTCT 
CTTGAGAGTT TGGGCAAAGG CATAAAGTTC CACTTCAACC CGGCTCAGTC GGTGTTGGTC 
ATGGAGGCTG ATGACTTGGA GAGCATCAAC ACAGCTATGA CAAAAGTTTC CTACATCAAC 
TCTCGACAGT TTCCAACTCC CGGGCTGCGC AAACTTCACA TTACCACAAC AGTCCAGTGT 
TTCGGTGAAG ACACTTGCAT CTCCATTCCA GAGATCAAGG CGATGGTGAT GGTTCTTCCG 
CCAAGTGAGC CTCGGATCAC CATTGCTGGC GTGGAGCGTC TGGTTTGGCC ATCGGCTCAG 
CTTCGAGCCC CAGTCGGAGT GGCTCTTTTC AAGGACATCC ACATCATTAG TACCGTCACC 
AAGACTGACG CCACCCTCAG CATAGCCCGG AGGCCTGGTG TCCTGGAGCT GATGCATAAC 
CTGGATTATT GTGATGTGCT GGTGATCGGT GAAGAGCTGG ACCCGGAGCG GGAGAGTCTG 
GAGATCCACC ACAGCTCTCT CCTGGGCAAA CATCTGGATG CCACCAACTC TACCTCTGGC 
ATCTCCATCT ACGGTGTGGA CTCGATGGCA CATTATGAAC AGGCCATCAG GCAGGTGAGG 
TACCGTAACT GGAAGCCCGG CAGTCTGTCG GAGAGAAGGT TCCGGCTCAG TTGCTCTGAA 
CTGAATGGAC GCTACACAAG CAATGAGTTC AACTTGGAGA TTGGAGTAGT TCACAGCTCA 
GAGGCCGTTG AGCATGTCAA TCACATGGCG GTCCAATCCC AGTTCATGAG ACCTGTGCAT 
CATCCACTGG TGGTTCACAC TGTCAACTCC GATCACATCT CAGGTACCCC TCCTGCAGCA 
ACAGTGGTAA TTGTAATGTG CATAGCTGCC CTGGTGGTCA TTGTGGTCTT GGGCATCTAC 
CGTATCCACA CCACCCATCA AGACAGCTCA AAAGAGGATG AAGAGGAAAG AAAAGACCCG 
GAGATGGACT GGGACAACTC CAATCTTAAT AGCATTGAAG GAACTCAGAT TGCAGAGGAG 
GTGAGAGAGG AAGAGCCAGA GGAAGATGAG GATGAAGACG AAGAAGATGA TGATTTGGCA 
GGAGATCTCT CTAGCGCTGA ATCTGAGGAC AGTGATGAAG ATGAAGAGAC TAACATTCAG 
AAGGGAAAAG TCAAAGGAAA GCTGGAGTGG GACCCGTCTA CGTTACCCTA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153311.1
CDS	1..2814
Translation	MKMRAITAMLLLVLSGQCGILAGKVNKHKPWIETSYHGVITENMDTVMLDPPLVALDKDAPVPYAGEICAFKIHGQEAPFEAEVLNRTSGEGVLRARGPIDCEQQKEYTFIIQAYDCGASPNGADWKKSHKAVVHIQVDDVNEFSPVFREPLYRATVTEGKIYDSILQVEAWDQDCSPQYSQICNYEIVTQDTPFAIDRNGNIRNTERLSFDKQQHYKIMVTAYDCGQKRAMESVPVHIDVKPVCKPGWQGWNKRVDYEPGTGSKQLFPKMHLETCDGPLSSVRAMVELQTSHIGKGCDRETYSEKSLQKLCGAASGSTDLLPAPSTSTNWTASLLTDSGRDSDLIFRFDGRQAANIPDWVVPQNLTDQFTIATWMKHGPSPGLRAEKETLLCNSDKTEMNRHHYSLYVHNCRLVFLLRRDFIQLDSFRPAEFHWRLEQICDKEWHYYVINVEFPSVTLFVDGVTYEPYLVTDDWPIHPSEIDVQLTVGACWQGGEVTTPRFTQYFRGSLSGLTIRPGKIATQKVISCLQACKEGLDISSLESLGKGIKFHFNPAQSVLVMEADDLESINTAMTKVSYINSRQFPTPGLRKLHITTTVQCFGEDTCISIPEIKAMVMVLPPSEPRITIAGVERLVWPSAQLRAPVGVALFKDIHIISTVTKTDATLSIARRPGVLELMHNLDYCDVLVIGEELDPERESLEIHHSSLLGKHLDATNSTSGISIYGVDSMAHYEQAIRQVRYRNWKPGSLSERRFRLSCSELNGRYTSNEFNLEIGVVHSSEAVEHVNHMAVQSQFMRPVHHPLVVHTVNSDHISGTPPAATVVIVMCIAALVVIVVLGIYRIHTTHQDSSKEDEEERKDPEMDWDNSNLNSIEGTQIAEEVREEEPEEDEDEDEEDDDLAGDLSSAESEDSDEDEETNIQKGKVKGKLEWDPSTLPY

Target ORF information:

RefSeq Version	NM_001159839.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calsyntenin 2 (clstn2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001159839.1

ORF Insert Sequence:

ATGAAGATGC GCGCGATAAC AGCGATGCTT CTCCTGGTGC TCTCCGGGCA GTGCGGCATC 
CTGGCTGGGA AAGTGAATAA GCACAAACCA TGGATTGAGA CATCCTACCA TGGAGTCATT 
ACTGAGAACA TGGACACTGT AATGCTGGAC CCTCCTCTTG TGGCGCTGGA CAAAGACGCT 
CCTGTTCCTT ATGCAGGAGA GATCTGTGCG TTTAAGATCC ATGGGCAGGA GGCGCCGTTT 
GAGGCTGAGG TGTTGAACAG GACATCAGGT GAAGGCGTGC TGAGGGCCCG GGGCCCTATA 
GACTGTGAGC AGCAGAAGGA GTACACCTTC ATCATCCAAG CCTATGACTG TGGGGCCAGT 
CCAAATGGAG CTGACTGGAA AAAGAGTCAC AAGGCGGTGG TTCATATCCA GGTGGATGAT 
GTAAACGAGT TCTCCCCAGT GTTCAGAGAG CCGTTGTATC GCGCCACTGT AACTGAAGGG 
AAGATCTATG ACAGCATCCT GCAGGTGGAG GCCTGGGATC AGGACTGCTC TCCACAGTAC 
AGCCAGATCT GCAACTATGA GATTGTCACA CAGGATACAC CCTTCGCCAT TGATCGAAAT 
GGCAACATAA GAAACACAGA GCGACTGAGC TTCGACAAAC AACAGCACTA TAAAATCATG 
GTGACGGCCT ACGATTGTGG CCAGAAGAGG GCGATGGAGA GCGTTCCAGT TCACATTGAC 
GTCAAACCAG TGTGCAAGCC AGGCTGGCAA GGTTGGAACA AGCGTGTAGA TTATGAACCT 
GGAACAGGCA GCAAGCAGCT CTTTCCCAAG ATGCACCTGG AGACCTGCGA TGGCCCCTTA 
TCCTCAGTGC GAGCAATGGT GGAGCTACAG ACCAGCCATA TTGGAAAGGG CTGCGACAGA 
GAGACCTATT CCGAGAAGTC TCTGCAGAAG CTTTGCGGAG CAGCCTCGGG CAGCACAGAC 
CTTCTCCCGG CTCCTAGCAC ATCCACCAAT TGGACGGCAT CCCTGCTGAC AGACAGTGGC 
CGAGACAGTG ACCTCATCTT CCGATTTGAT GGACGACAGG CAGCCAACAT CCCAGATTGG 
GTTGTGCCAC AGAACCTGAC AGATCAGTTC ACCATAGCAA CATGGATGAA GCATGGGCCG 
AGTCCTGGAC TGAGAGCTGA GAAGGAGACG TTGCTCTGTA ATTCAGATAA AACTGAGATG 
AACAGACATC ATTATTCTCT GTATGTCCAC AACTGCCGTC TGGTGTTTCT TCTGAGGCGC 
GACTTCATTC AGCTGGACTC CTTCAGACCG GCCGAATTTC ACTGGAGACT GGAACAGATC 
TGTGATAAAG AGTGGCATTA CTACGTCATC AATGTGGAGT TTCCATCAGT AACCCTCTTC 
GTAGACGGAG TGACATACGA ACCATACCTG GTGACAGACG ACTGGCCCAT CCACCCTTCA 
GAGATCGATG TACAGCTGAC CGTCGGCGCC TGCTGGCAAG GAGGAGAAGT GACCACTCCA 
AGGTTTACAC AGTATTTCCG AGGAAGCCTC TCGGGGTTGA CTATCCGACC AGGGAAAATC 
GCCACTCAGA AGGTGATCTC ATGCCTACAG GCTTGCAAGG AAGGTCTCGA CATCAGCTCT 
CTTGAGAGTT TGGGCAAAGG CATAAAGTTC CACTTCAACC CGGCTCAGTC GGTGTTGGTC 
ATGGAGGCTG ATGACTTGGA GAGCATCAAC ACAGCTATGA CAAAAGTTTC CTACATCAAC 
TCTCGACAGT TTCCAACTCC CGGGCTGCGC AAACTTCACA TTACCACAAC AGTCCAGTGT 
TTCGGTGAAG ACACTTGCAT CTCCATTCCA GAGATCAAGG CGATGGTGAT GGTTCTTCCG 
CCAAGTGAGC CTCGGATCAC CATTGCTGGC GTGGAGCGTC TGGTTTGGCC ATCGGCTCAG 
CTTCGAGCCC CAGTCGGAGT GGCTCTTTTC AAGGACATCC ACATCATTAG TACCGTCACC 
AAGACTGACG CCACCCTCAG CATAGCCCGG AGGCCTGGTG TCCTGGAGCT GATGCATAAC 
CTGGATTATT GTGATGTGCT GGTGATCGGT GAAGAGCTGG ACCCGGAGCG GGAGAGTCTG 
GAGATCCACC ACAGCTCTCT CCTGGGCAAA CATCTGGATG CCACCAACTC TACCTCTGGC 
ATCTCCATCT ACGGTGTGGA CTCGATGGCA CATTATGAAC AGGCCATCAG GCAGGTGAGG 
TACCGTAACT GGAAGCCCGG CAGTCTGTCG GAGAGAAGGT TCCGGCTCAG TTGCTCTGAA 
CTGAATGGAC GCTACACAAG CAATGAGTTC AACTTGGAGA TTGGAGTAGT TCACAGCTCA 
GAGGCCGTTG AGCATGTCAA TCACATGGCG GTCCAATCCC AGTTCATGAG ACCTGTGCAT 
CATCCACTGG TGGTTCACAC TGTCAACTCC GATCACATCT CAGGTACCCC TCCTGCAGCA 
ACAGTGGTAA TTGTAATGTG CATAGCTGCC CTGGTGGTCA TTGTGGTCTT GGGCATCTAC 
CGTATCCACA CCACCCATCA AGACAGCTCA AAAGAGGATG AAGAGGAAAG AAAAGACCCG 
GAGATGGACT GGGACAACTC CAATCTTAAT AGCATTGAAG GAACTCAGAT TGCAGAGGAG 
GTGAGAGAGG AAGAGCCAGA GGAAGATGAG GATGAAGACG AAGAAGATGA TGATTTGGCA 
GGAGATCTCT CTAGCGCTGA ATCTGAGGAC AGTGATGAAG ATGAAGAGAC TAACATTCAG 
AAGGGAAAAG TCAAAGGAAA GCTGGAGTGG GACCCGTCTA CGTTACCCTA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

clstn2 ( XM_009298708.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296983.1 Danio rerio calsyntenin 2 (clstn2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa48468
Clone ID Related Accession (Same CDS sequence)	XM_009298708.2 , XM_009298708.3
Accession Version	XM_009298708.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2835bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	calsyntenin-2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298708.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATGC GCGCGATAAC AGCGATGCTT CTCCTGGTGC TCTCCGGGCA GTGCGGCATC 
CTGGCTGGGA AAGTGAATAA GCACAAACCA TGGATTGAGA CATCCTACCA TGGAGTCATT 
ACTGAGAACA TGGACACTGT AATGCTGGAC CCTCCTCTTG TGGCGCTGGA CAAAGACGCT 
CCTGTTCCTT ATGCAGGAGA GATCTGTGCG TTTAAGATCC ATGGGCAGGA GGCGCCGTTT 
GAGGCTGAGG TGTTGAACAG GACATCAGGT GAAGGCGTGC TGAGGGCCCG GGGCCCTATA 
GACTGTGAGC AGCAGAAGGA GTACACCTTC ATCATCCAAG CCTATGACTG TGGGGCCAGT 
CCAAATGGAG CTGACTGGAA AAAGAGTCAC AAGGCGGTGG TTCATATCCA GGTGGATGAT 
GTAAACGAGT TCTCCCCAGT GTTCAGAGAG CCGTTGTATC GCGCCACTGT AACTGAAGGG 
AAGATCTATG ACAGCATCCT GCAGGTGGAG GCCTGGGATC AGGACTGCTC TCCACAGTAC 
AGCCAGATCT GCAACTATGA GATTGTCACA CAGGATACAC CCTTCGCCAT TGATCGAAAT 
GGCAACATAA GAAACACAGA GCGACTGAGC TTCGACAAAC AACAGCACTA TAAAATCATG 
GTGACGGCCT ACGATTGTGG CCAGAAGAGG GCGATGGAGA GCGTTCCAGT TCACATTGAC 
GTCAAACCAG TGTGCAAGCC AGGCTGGCAA GGTTGGAACA AGCGTGTAGA TTATGAACCT 
GGAACAGGCA GCAAGCAGCT CTTTCCCAAG ATGCACCTGG AGACCTGCGA TGGCCCCTTA 
TCCTCAGTGC GAGCAATGGT GGAGCTACAG ACCAGCCATA TTGGAAAGGG CTGCGACAGA 
GAGACCTATT CCGAGAAGTC TCTGCAGAAG CTTTGCGGAG CAGCCTCGGG CAGCACAGAC 
CTTCTCCCGG CTCCTAGCAC ATCCACCAAT TGGACGGCAT CCCTGCTGAC AGACAGTGGC 
CGAGACAGTG ACCTCATCTT CCGATTTGAT GGACGACAGG CAGCCAACAT CCCAGATTGG 
GTTGTGCCAC AGAACCTGAC AGATCAGTTC ACCATAGCAA CATGGATGAA GCATGGGCCG 
AGTCCTGGAC TGAGAGCTGA GAAGGAGACG TTGCTCTGTA ATTCAGATAA AACTGAGATG 
AACAGACATC ATTATTCTCT GTATGTCCAC AACTGCCGTC TGGTGTTTCT TCTGAGGCGC 
GACTTCATTC AGCTGGACTC CTTCAGACCG GCCGAATTTC ACTGGAGACT GGAACAGATC 
TGTGATAAAG AGTGGCATTA CTACGTCATC AATGTGGAGT TTCCATCAGT AACCCTCTTC 
GTAGACGGAG TGACATACGA ACCATACCTG GTGACAGACG ACTGGCCCAT CCACCCTTCA 
GAGATCGATG TACAGCTGAC CGTCGGCGCC TGCTGGCAAG GAGGAGAAGT GACCACTCCA 
AGGTTTACAC AGTATTTCCG AGGAAGCCTC TCGGGGTTGA CTATCCGACC AGGGAAAATC 
GCCACTCAGA AGGTGATCTC ATGCCTACAG GCTTGCAAGG AAGGTCTCGA CATCAGCTCT 
CTTGAGAGTT TGGGCAAAGG CATAAAGTTC CACTTCAACC CGGCTCAGTC GGTGTTGGTC 
ATGGAGGCTG ATGACTTGGA GAGCATCAAC ACAGCTATGA CAAAAGTTTC CTACATCAAC 
TCTCGACAGT TTCCAACTCC CGGGCTGCGC AAACTTCACA TTACCACAAC AGTCCAGTGT 
TTCGGTGAAG ACACTTGCAT CTCCATTCCA GAGATCAAGG CGATGGTGAT GGTTCTTCCG 
CCAAGTGAGC CTCGGATCAC CATTGCTGGC GTGGAGCGTC TGGTTTGGCC ATCGGCTCAG 
CTTCGAGCCC CAGTCGGAGT GGCTCTTTTC AAGGACATCC ACATCATTAG TACCGTCACC 
AAGACTGACG CCACCCTCAG CATAGCCCGG AGGCCTGGTG TCCTGGAGCT GATGCATAAC 
CTGGATTATT GTGATGTGCT GGTGATCGGT GAAGAGCTGG ACCCGGAGCG GGAGAGTCTG 
GAGATCCACC ACAGCTCTCT CCTGGGCAAA CATCTGGATG CCACCAACTC TACCTCTGGC 
ATCTCCATCT ACGGTGTGGA CTCGATGGCA CATTATGAAC AGGCCATCAG GCAGGTGAGG 
TACCGTAACT GGAAGCCCGG CAGTCTGTCG GAGAGAAGGT TCCGGCTCAG TTGCTCTGAA 
CTGAATGGAC GCTACACAAG CAATGAGTTC AACTTGGAGA TTGGAGTAGT TCACAGCTCA 
GAGGCCGTTG AGCATGTCAA TCACATGGCG GTCCAATCCC AGTTCATGAG ACCTGTGCAT 
CATCCACTGG TGGTTCACAC TGTCAACTCC GATCACATCT CAGGTACCCC TCCTGCAGCA 
ACAGTGGTAA TTGTAATGTG CATAGCTGCC CTGGTGGTCA TTGTGGTCTT GGGCATCTAC 
CGTATCCACA CCACCCATCA AGACAGCTCA AAAGAGGATG AAGAGGAAAG AAAAGACCCG 
GAGATGGACT GGGACAACTC CAATCTTAAC ATCACTGTTA ATCCCATGGA GAGCATTGAA 
GGAACTCAGA TTGCAGAGGA GGTGAGAGAG GAAGAGCCAG AGGAAGATGA GGATGAAGAC 
GAAGAAGATG ATGATTTGGC AGGAGATCTC TCTAGCGCTG AATCTGAGGA CAGTGATGAA 
GATGAAGAGA CTAACATTCA GAAGGGAAAA GTCAAAGGAA AGCTGGAGTG GGACCCGTCT 
ACGTTACCCT ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296983.1
CDS	251..3085
Translation	MKMRAITAMLLLVLSGQCGILAGKVNKHKPWIETSYHGVITENMDTVMLDPPLVALDKDAPVPYAGEICAFKIHGQEAPFEAEVLNRTSGEGVLRARGPIDCEQQKEYTFIIQAYDCGASPNGADWKKSHKAVVHIQVDDVNEFSPVFREPLYRATVTEGKIYDSILQVEAWDQDCSPQYSQICNYEIVTQDTPFAIDRNGNIRNTERLSFDKQQHYKIMVTAYDCGQKRAMESVPVHIDVKPVCKPGWQGWNKRVDYEPGTGSKQLFPKMHLETCDGPLSSVRAMVELQTSHIGKGCDRETYSEKSLQKLCGAASGSTDLLPAPSTSTNWTASLLTDSGRDSDLIFRFDGRQAANIPDWVVPQNLTDQFTIATWMKHGPSPGLRAEKETLLCNSDKTEMNRHHYSLYVHNCRLVFLLRRDFIQLDSFRPAEFHWRLEQICDKEWHYYVINVEFPSVTLFVDGVTYEPYLVTDDWPIHPSEIDVQLTVGACWQGGEVTTPRFTQYFRGSLSGLTIRPGKIATQKVISCLQACKEGLDISSLESLGKGIKFHFNPAQSVLVMEADDLESINTAMTKVSYINSRQFPTPGLRKLHITTTVQCFGEDTCISIPEIKAMVMVLPPSEPRITIAGVERLVWPSAQLRAPVGVALFKDIHIISTVTKTDATLSIARRPGVLELMHNLDYCDVLVIGEELDPERESLEIHHSSLLGKHLDATNSTSGISIYGVDSMAHYEQAIRQVRYRNWKPGSLSERRFRLSCSELNGRYTSNEFNLEIGVVHSSEAVEHVNHMAVQSQFMRPVHHPLVVHTVNSDHISGTPPAATVVIVMCIAALVVIVVLGIYRIHTTHQDSSKEDEEERKDPEMDWDNSNLNITVNPMESIEGTQIAEEVREEEPEEDEDEDEEDDDLAGDLSSAESEDSDEDEETNIQKGKVKGKLEWDPSTLPY

Target ORF information:

RefSeq Version	XM_009298708.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calsyntenin 2 (clstn2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298708.2

ORF Insert Sequence:

ATGAAGATGC GCGCGATAAC AGCGATGCTT CTCCTGGTGC TCTCCGGGCA GTGCGGCATC 
CTGGCTGGGA AAGTGAATAA GCACAAACCA TGGATTGAGA CATCCTACCA TGGAGTCATT 
ACTGAGAACA TGGACACTGT AATGCTGGAC CCTCCTCTTG TGGCGCTGGA CAAAGACGCT 
CCTGTTCCTT ATGCAGGAGA GATCTGTGCG TTTAAGATCC ATGGGCAGGA GGCGCCGTTT 
GAGGCTGAGG TGTTGAACAG GACATCAGGT GAAGGCGTGC TGAGGGCCCG GGGCCCTATA 
GACTGTGAGC AGCAGAAGGA GTACACCTTC ATCATCCAAG CCTATGACTG TGGGGCCAGT 
CCAAATGGAG CTGACTGGAA AAAGAGTCAC AAGGCGGTGG TTCATATCCA GGTGGATGAT 
GTAAACGAGT TCTCCCCAGT GTTCAGAGAG CCGTTGTATC GCGCCACTGT AACTGAAGGG 
AAGATCTATG ACAGCATCCT GCAGGTGGAG GCCTGGGATC AGGACTGCTC TCCACAGTAC 
AGCCAGATCT GCAACTATGA GATTGTCACA CAGGATACAC CCTTCGCCAT TGATCGAAAT 
GGCAACATAA GAAACACAGA GCGACTGAGC TTCGACAAAC AACAGCACTA TAAAATCATG 
GTGACGGCCT ACGATTGTGG CCAGAAGAGG GCGATGGAGA GCGTTCCAGT TCACATTGAC 
GTCAAACCAG TGTGCAAGCC AGGCTGGCAA GGTTGGAACA AGCGTGTAGA TTATGAACCT 
GGAACAGGCA GCAAGCAGCT CTTTCCCAAG ATGCACCTGG AGACCTGCGA TGGCCCCTTA 
TCCTCAGTGC GAGCAATGGT GGAGCTACAG ACCAGCCATA TTGGAAAGGG CTGCGACAGA 
GAGACCTATT CCGAGAAGTC TCTGCAGAAG CTTTGCGGAG CAGCCTCGGG CAGCACAGAC 
CTTCTCCCGG CTCCTAGCAC ATCCACCAAT TGGACGGCAT CCCTGCTGAC AGACAGTGGC 
CGAGACAGTG ACCTCATCTT CCGATTTGAT GGACGACAGG CAGCCAACAT CCCAGATTGG 
GTTGTGCCAC AGAACCTGAC AGATCAGTTC ACCATAGCAA CATGGATGAA GCATGGGCCG 
AGTCCTGGAC TGAGAGCTGA GAAGGAGACG TTGCTCTGTA ATTCAGATAA AACTGAGATG 
AACAGACATC ATTATTCTCT GTATGTCCAC AACTGCCGTC TGGTGTTTCT TCTGAGGCGC 
GACTTCATTC AGCTGGACTC CTTCAGACCG GCCGAATTTC ACTGGAGACT GGAACAGATC 
TGTGATAAAG AGTGGCATTA CTACGTCATC AATGTGGAGT TTCCATCAGT AACCCTCTTC 
GTAGACGGAG TGACATACGA ACCATACCTG GTGACAGACG ACTGGCCCAT CCACCCTTCA 
GAGATCGATG TACAGCTGAC CGTCGGCGCC TGCTGGCAAG GAGGAGAAGT GACCACTCCA 
AGGTTTACAC AGTATTTCCG AGGAAGCCTC TCGGGGTTGA CTATCCGACC AGGGAAAATC 
GCCACTCAGA AGGTGATCTC ATGCCTACAG GCTTGCAAGG AAGGTCTCGA CATCAGCTCT 
CTTGAGAGTT TGGGCAAAGG CATAAAGTTC CACTTCAACC CGGCTCAGTC GGTGTTGGTC 
ATGGAGGCTG ATGACTTGGA GAGCATCAAC ACAGCTATGA CAAAAGTTTC CTACATCAAC 
TCTCGACAGT TTCCAACTCC CGGGCTGCGC AAACTTCACA TTACCACAAC AGTCCAGTGT 
TTCGGTGAAG ACACTTGCAT CTCCATTCCA GAGATCAAGG CGATGGTGAT GGTTCTTCCG 
CCAAGTGAGC CTCGGATCAC CATTGCTGGC GTGGAGCGTC TGGTTTGGCC ATCGGCTCAG 
CTTCGAGCCC CAGTCGGAGT GGCTCTTTTC AAGGACATCC ACATCATTAG TACCGTCACC 
AAGACTGACG CCACCCTCAG CATAGCCCGG AGGCCTGGTG TCCTGGAGCT GATGCATAAC 
CTGGATTATT GTGATGTGCT GGTGATCGGT GAAGAGCTGG ACCCGGAGCG GGAGAGTCTG 
GAGATCCACC ACAGCTCTCT CCTGGGCAAA CATCTGGATG CCACCAACTC TACCTCTGGC 
ATCTCCATCT ACGGTGTGGA CTCGATGGCA CATTATGAAC AGGCCATCAG GCAGGTGAGG 
TACCGTAACT GGAAGCCCGG CAGTCTGTCG GAGAGAAGGT TCCGGCTCAG TTGCTCTGAA 
CTGAATGGAC GCTACACAAG CAATGAGTTC AACTTGGAGA TTGGAGTAGT TCACAGCTCA 
GAGGCCGTTG AGCATGTCAA TCACATGGCG GTCCAATCCC AGTTCATGAG ACCTGTGCAT 
CATCCACTGG TGGTTCACAC TGTCAACTCC GATCACATCT CAGGTACCCC TCCTGCAGCA 
ACAGTGGTAA TTGTAATGTG CATAGCTGCC CTGGTGGTCA TTGTGGTCTT GGGCATCTAC 
CGTATCCACA CCACCCATCA AGACAGCTCA AAAGAGGATG AAGAGGAAAG AAAAGACCCG 
GAGATGGACT GGGACAACTC CAATCTTAAC ATCACTGTTA ATCCCATGGA GAGCATTGAA 
GGAACTCAGA TTGCAGAGGA GGTGAGAGAG GAAGAGCCAG AGGAAGATGA GGATGAAGAC 
GAAGAAGATG ATGATTTGGC AGGAGATCTC TCTAGCGCTG AATCTGAGGA CAGTGATGAA 
GATGAAGAGA CTAACATTCA GAAGGGAAAA GTCAAAGGAA AGCTGGAGTG GGACCCGTCT 
ACGTTACCCT ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

clstn2 ( XM_009298708.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296983.1 Danio rerio calsyntenin 2 (clstn2), transcript variant X1, mRNA.

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CloneID	ODa48468
Clone ID Related Accession (Same CDS sequence)	XM_009298708.2 , XM_009298708.3
Accession Version	XM_009298708.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2835bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	calsyntenin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298708.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATGC GCGCGATAAC AGCGATGCTT CTCCTGGTGC TCTCCGGGCA GTGCGGCATC 
CTGGCTGGGA AAGTGAATAA GCACAAACCA TGGATTGAGA CATCCTACCA TGGAGTCATT 
ACTGAGAACA TGGACACTGT AATGCTGGAC CCTCCTCTTG TGGCGCTGGA CAAAGACGCT 
CCTGTTCCTT ATGCAGGAGA GATCTGTGCG TTTAAGATCC ATGGGCAGGA GGCGCCGTTT 
GAGGCTGAGG TGTTGAACAG GACATCAGGT GAAGGCGTGC TGAGGGCCCG GGGCCCTATA 
GACTGTGAGC AGCAGAAGGA GTACACCTTC ATCATCCAAG CCTATGACTG TGGGGCCAGT 
CCAAATGGAG CTGACTGGAA AAAGAGTCAC AAGGCGGTGG TTCATATCCA GGTGGATGAT 
GTAAACGAGT TCTCCCCAGT GTTCAGAGAG CCGTTGTATC GCGCCACTGT AACTGAAGGG 
AAGATCTATG ACAGCATCCT GCAGGTGGAG GCCTGGGATC AGGACTGCTC TCCACAGTAC 
AGCCAGATCT GCAACTATGA GATTGTCACA CAGGATACAC CCTTCGCCAT TGATCGAAAT 
GGCAACATAA GAAACACAGA GCGACTGAGC TTCGACAAAC AACAGCACTA TAAAATCATG 
GTGACGGCCT ACGATTGTGG CCAGAAGAGG GCGATGGAGA GCGTTCCAGT TCACATTGAC 
GTCAAACCAG TGTGCAAGCC AGGCTGGCAA GGTTGGAACA AGCGTGTAGA TTATGAACCT 
GGAACAGGCA GCAAGCAGCT CTTTCCCAAG ATGCACCTGG AGACCTGCGA TGGCCCCTTA 
TCCTCAGTGC GAGCAATGGT GGAGCTACAG ACCAGCCATA TTGGAAAGGG CTGCGACAGA 
GAGACCTATT CCGAGAAGTC TCTGCAGAAG CTTTGCGGAG CAGCCTCGGG CAGCACAGAC 
CTTCTCCCGG CTCCTAGCAC ATCCACCAAT TGGACGGCAT CCCTGCTGAC AGACAGTGGC 
CGAGACAGTG ACCTCATCTT CCGATTTGAT GGACGACAGG CAGCCAACAT CCCAGATTGG 
GTTGTGCCAC AGAACCTGAC AGATCAGTTC ACCATAGCAA CATGGATGAA GCATGGGCCG 
AGTCCTGGAC TGAGAGCTGA GAAGGAGACG TTGCTCTGTA ATTCAGATAA AACTGAGATG 
AACAGACATC ATTATTCTCT GTATGTCCAC AACTGCCGTC TGGTGTTTCT TCTGAGGCGC 
GACTTCATTC AGCTGGACTC CTTCAGACCG GCCGAATTTC ACTGGAGACT GGAACAGATC 
TGTGATAAAG AGTGGCATTA CTACGTCATC AATGTGGAGT TTCCATCAGT AACCCTCTTC 
GTAGACGGAG TGACATACGA ACCATACCTG GTGACAGACG ACTGGCCCAT CCACCCTTCA 
GAGATCGATG TACAGCTGAC CGTCGGCGCC TGCTGGCAAG GAGGAGAAGT GACCACTCCA 
AGGTTTACAC AGTATTTCCG AGGAAGCCTC TCGGGGTTGA CTATCCGACC AGGGAAAATC 
GCCACTCAGA AGGTGATCTC ATGCCTACAG GCTTGCAAGG AAGGTCTCGA CATCAGCTCT 
CTTGAGAGTT TGGGCAAAGG CATAAAGTTC CACTTCAACC CGGCTCAGTC GGTGTTGGTC 
ATGGAGGCTG ATGACTTGGA GAGCATCAAC ACAGCTATGA CAAAAGTTTC CTACATCAAC 
TCTCGACAGT TTCCAACTCC CGGGCTGCGC AAACTTCACA TTACCACAAC AGTCCAGTGT 
TTCGGTGAAG ACACTTGCAT CTCCATTCCA GAGATCAAGG CGATGGTGAT GGTTCTTCCG 
CCAAGTGAGC CTCGGATCAC CATTGCTGGC GTGGAGCGTC TGGTTTGGCC ATCGGCTCAG 
CTTCGAGCCC CAGTCGGAGT GGCTCTTTTC AAGGACATCC ACATCATTAG TACCGTCACC 
AAGACTGACG CCACCCTCAG CATAGCCCGG AGGCCTGGTG TCCTGGAGCT GATGCATAAC 
CTGGATTATT GTGATGTGCT GGTGATCGGT GAAGAGCTGG ACCCGGAGCG GGAGAGTCTG 
GAGATCCACC ACAGCTCTCT CCTGGGCAAA CATCTGGATG CCACCAACTC TACCTCTGGC 
ATCTCCATCT ACGGTGTGGA CTCGATGGCA CATTATGAAC AGGCCATCAG GCAGGTGAGG 
TACCGTAACT GGAAGCCCGG CAGTCTGTCG GAGAGAAGGT TCCGGCTCAG TTGCTCTGAA 
CTGAATGGAC GCTACACAAG CAATGAGTTC AACTTGGAGA TTGGAGTAGT TCACAGCTCA 
GAGGCCGTTG AGCATGTCAA TCACATGGCG GTCCAATCCC AGTTCATGAG ACCTGTGCAT 
CATCCACTGG TGGTTCACAC TGTCAACTCC GATCACATCT CAGGTACCCC TCCTGCAGCA 
ACAGTGGTAA TTGTAATGTG CATAGCTGCC CTGGTGGTCA TTGTGGTCTT GGGCATCTAC 
CGTATCCACA CCACCCATCA AGACAGCTCA AAAGAGGATG AAGAGGAAAG AAAAGACCCG 
GAGATGGACT GGGACAACTC CAATCTTAAC ATCACTGTTA ATCCCATGGA GAGCATTGAA 
GGAACTCAGA TTGCAGAGGA GGTGAGAGAG GAAGAGCCAG AGGAAGATGA GGATGAAGAC 
GAAGAAGATG ATGATTTGGC AGGAGATCTC TCTAGCGCTG AATCTGAGGA CAGTGATGAA 
GATGAAGAGA CTAACATTCA GAAGGGAAAA GTCAAAGGAA AGCTGGAGTG GGACCCGTCT 
ACGTTACCCT ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296983.1
CDS	218..3052
Translation	MKMRAITAMLLLVLSGQCGILAGKVNKHKPWIETSYHGVITENMDTVMLDPPLVALDKDAPVPYAGEICAFKIHGQEAPFEAEVLNRTSGEGVLRARGPIDCEQQKEYTFIIQAYDCGASPNGADWKKSHKAVVHIQVDDVNEFSPVFREPLYRATVTEGKIYDSILQVEAWDQDCSPQYSQICNYEIVTQDTPFAIDRNGNIRNTERLSFDKQQHYKIMVTAYDCGQKRAMESVPVHIDVKPVCKPGWQGWNKRVDYEPGTGSKQLFPKMHLETCDGPLSSVRAMVELQTSHIGKGCDRETYSEKSLQKLCGAASGSTDLLPAPSTSTNWTASLLTDSGRDSDLIFRFDGRQAANIPDWVVPQNLTDQFTIATWMKHGPSPGLRAEKETLLCNSDKTEMNRHHYSLYVHNCRLVFLLRRDFIQLDSFRPAEFHWRLEQICDKEWHYYVINVEFPSVTLFVDGVTYEPYLVTDDWPIHPSEIDVQLTVGACWQGGEVTTPRFTQYFRGSLSGLTIRPGKIATQKVISCLQACKEGLDISSLESLGKGIKFHFNPAQSVLVMEADDLESINTAMTKVSYINSRQFPTPGLRKLHITTTVQCFGEDTCISIPEIKAMVMVLPPSEPRITIAGVERLVWPSAQLRAPVGVALFKDIHIISTVTKTDATLSIARRPGVLELMHNLDYCDVLVIGEELDPERESLEIHHSSLLGKHLDATNSTSGISIYGVDSMAHYEQAIRQVRYRNWKPGSLSERRFRLSCSELNGRYTSNEFNLEIGVVHSSEAVEHVNHMAVQSQFMRPVHHPLVVHTVNSDHISGTPPAATVVIVMCIAALVVIVVLGIYRIHTTHQDSSKEDEEERKDPEMDWDNSNLNITVNPMESIEGTQIAEEVREEEPEEDEDEDEEDDDLAGDLSSAESEDSDEDEETNIQKGKVKGKLEWDPSTLPY

Target ORF information:

RefSeq Version	XM_009298708.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calsyntenin 2 (clstn2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298708.3

ORF Insert Sequence:

ATGAAGATGC GCGCGATAAC AGCGATGCTT CTCCTGGTGC TCTCCGGGCA GTGCGGCATC 
CTGGCTGGGA AAGTGAATAA GCACAAACCA TGGATTGAGA CATCCTACCA TGGAGTCATT 
ACTGAGAACA TGGACACTGT AATGCTGGAC CCTCCTCTTG TGGCGCTGGA CAAAGACGCT 
CCTGTTCCTT ATGCAGGAGA GATCTGTGCG TTTAAGATCC ATGGGCAGGA GGCGCCGTTT 
GAGGCTGAGG TGTTGAACAG GACATCAGGT GAAGGCGTGC TGAGGGCCCG GGGCCCTATA 
GACTGTGAGC AGCAGAAGGA GTACACCTTC ATCATCCAAG CCTATGACTG TGGGGCCAGT 
CCAAATGGAG CTGACTGGAA AAAGAGTCAC AAGGCGGTGG TTCATATCCA GGTGGATGAT 
GTAAACGAGT TCTCCCCAGT GTTCAGAGAG CCGTTGTATC GCGCCACTGT AACTGAAGGG 
AAGATCTATG ACAGCATCCT GCAGGTGGAG GCCTGGGATC AGGACTGCTC TCCACAGTAC 
AGCCAGATCT GCAACTATGA GATTGTCACA CAGGATACAC CCTTCGCCAT TGATCGAAAT 
GGCAACATAA GAAACACAGA GCGACTGAGC TTCGACAAAC AACAGCACTA TAAAATCATG 
GTGACGGCCT ACGATTGTGG CCAGAAGAGG GCGATGGAGA GCGTTCCAGT TCACATTGAC 
GTCAAACCAG TGTGCAAGCC AGGCTGGCAA GGTTGGAACA AGCGTGTAGA TTATGAACCT 
GGAACAGGCA GCAAGCAGCT CTTTCCCAAG ATGCACCTGG AGACCTGCGA TGGCCCCTTA 
TCCTCAGTGC GAGCAATGGT GGAGCTACAG ACCAGCCATA TTGGAAAGGG CTGCGACAGA 
GAGACCTATT CCGAGAAGTC TCTGCAGAAG CTTTGCGGAG CAGCCTCGGG CAGCACAGAC 
CTTCTCCCGG CTCCTAGCAC ATCCACCAAT TGGACGGCAT CCCTGCTGAC AGACAGTGGC 
CGAGACAGTG ACCTCATCTT CCGATTTGAT GGACGACAGG CAGCCAACAT CCCAGATTGG 
GTTGTGCCAC AGAACCTGAC AGATCAGTTC ACCATAGCAA CATGGATGAA GCATGGGCCG 
AGTCCTGGAC TGAGAGCTGA GAAGGAGACG TTGCTCTGTA ATTCAGATAA AACTGAGATG 
AACAGACATC ATTATTCTCT GTATGTCCAC AACTGCCGTC TGGTGTTTCT TCTGAGGCGC 
GACTTCATTC AGCTGGACTC CTTCAGACCG GCCGAATTTC ACTGGAGACT GGAACAGATC 
TGTGATAAAG AGTGGCATTA CTACGTCATC AATGTGGAGT TTCCATCAGT AACCCTCTTC 
GTAGACGGAG TGACATACGA ACCATACCTG GTGACAGACG ACTGGCCCAT CCACCCTTCA 
GAGATCGATG TACAGCTGAC CGTCGGCGCC TGCTGGCAAG GAGGAGAAGT GACCACTCCA 
AGGTTTACAC AGTATTTCCG AGGAAGCCTC TCGGGGTTGA CTATCCGACC AGGGAAAATC 
GCCACTCAGA AGGTGATCTC ATGCCTACAG GCTTGCAAGG AAGGTCTCGA CATCAGCTCT 
CTTGAGAGTT TGGGCAAAGG CATAAAGTTC CACTTCAACC CGGCTCAGTC GGTGTTGGTC 
ATGGAGGCTG ATGACTTGGA GAGCATCAAC ACAGCTATGA CAAAAGTTTC CTACATCAAC 
TCTCGACAGT TTCCAACTCC CGGGCTGCGC AAACTTCACA TTACCACAAC AGTCCAGTGT 
TTCGGTGAAG ACACTTGCAT CTCCATTCCA GAGATCAAGG CGATGGTGAT GGTTCTTCCG 
CCAAGTGAGC CTCGGATCAC CATTGCTGGC GTGGAGCGTC TGGTTTGGCC ATCGGCTCAG 
CTTCGAGCCC CAGTCGGAGT GGCTCTTTTC AAGGACATCC ACATCATTAG TACCGTCACC 
AAGACTGACG CCACCCTCAG CATAGCCCGG AGGCCTGGTG TCCTGGAGCT GATGCATAAC 
CTGGATTATT GTGATGTGCT GGTGATCGGT GAAGAGCTGG ACCCGGAGCG GGAGAGTCTG 
GAGATCCACC ACAGCTCTCT CCTGGGCAAA CATCTGGATG CCACCAACTC TACCTCTGGC 
ATCTCCATCT ACGGTGTGGA CTCGATGGCA CATTATGAAC AGGCCATCAG GCAGGTGAGG 
TACCGTAACT GGAAGCCCGG CAGTCTGTCG GAGAGAAGGT TCCGGCTCAG TTGCTCTGAA 
CTGAATGGAC GCTACACAAG CAATGAGTTC AACTTGGAGA TTGGAGTAGT TCACAGCTCA 
GAGGCCGTTG AGCATGTCAA TCACATGGCG GTCCAATCCC AGTTCATGAG ACCTGTGCAT 
CATCCACTGG TGGTTCACAC TGTCAACTCC GATCACATCT CAGGTACCCC TCCTGCAGCA 
ACAGTGGTAA TTGTAATGTG CATAGCTGCC CTGGTGGTCA TTGTGGTCTT GGGCATCTAC 
CGTATCCACA CCACCCATCA AGACAGCTCA AAAGAGGATG AAGAGGAAAG AAAAGACCCG 
GAGATGGACT GGGACAACTC CAATCTTAAC ATCACTGTTA ATCCCATGGA GAGCATTGAA 
GGAACTCAGA TTGCAGAGGA GGTGAGAGAG GAAGAGCCAG AGGAAGATGA GGATGAAGAC 
GAAGAAGATG ATGATTTGGC AGGAGATCTC TCTAGCGCTG AATCTGAGGA CAGTGATGAA 
GATGAAGAGA CTAACATTCA GAAGGGAAAA GTCAAAGGAA AGCTGGAGTG GGACCCGTCT 
ACGTTACCCT ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequential, Divergent, and Cooperative Requirements of
Genetics205(4)1551-1572(2017 04)
Yang YJ,Wang Y,Li Z,Zhou L,Gui JF

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Zebrafish calsyntenins mediate homophilic adhesion through their amino-terminal cadherin repeats.
Neuroscience28687-96(2015 Feb)
Ortiz-Medina H,Emond MR,Jontes JD

Identification of zygotic genes expressed at the midblastula transition in zebrafish.
Biochemical and biophysical research communications358(2)462-8(2007 Jun)
O'Boyle S,Bree RT,McLoughlin S,Grealy M,Byrnes L

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clstn2 cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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