plod2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	plod2
Entrez Gene ID	100036767
Full Name	procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2
Synonyms	lh2,zgc:101868
General protein information	Preferred Names procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2 Names procollagen-lysine,2-oxoglutarate 5-dioxygenase 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001089464.1	NP_001082933.1	procollagen-lysine,2-oxoglutarate 5-dioxygenase 2 isoform 2 precursor
NM_001007377.2	NP_001007378.2	procollagen-lysine,2-oxoglutarate 5-dioxygenase 2 isoform 1 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PLOD2	XP_542822.4
Bos taurus (cattle)	PLOD2	NP_001094619.1
Danio rerio (zebrafish)	plod2	NP_001082933.1
Macaca mulatta (Rhesus monkey)	LOC715820	XP_002808385.1
Mus musculus (house mouse)	Plod2	NP_001136388.1
Rattus norvegicus (Norway rat)	Plod2	NP_787065.2
Homo sapiens (human)	PLOD2	NP_891988.1
Xenopus tropicalis (tropical clawed frog)	plod2	XP_004914412.1
Pan troglodytes (chimpanzee)	PLOD2	XP_516801.3
Gallus gallus (chicken)	PLOD2	XP_004943367.1

Gene Ontology
Bibliography

Related articles in PubMed

Zebrafish type I collagen mutants faithfully recapitulate human type I collagenopathies.
Gistelinck C, Kwon RY, Malfait F, Symoens S, Harris MP, Henke K, Hawkins MB, Fisher S, Sips P, Guillemyn B, Bek JW, Vermassen P, De Saffel H, Witten PE, Weis M, De Paepe A, Eyre DR, Willaert A, Coucke PJ
Proceedings of the National Academy of Sciences of the United States of America115(34)E8037-E8046(2018 08)

MicroCT-based phenomics in the zebrafish skeleton reveals virtues of deep phenotyping in a distributed organ system.
Hur M, Gistelinck CA, Huber P, Lee J, Thompson MH, Monstad-Rios AT, Watson CJ, McMenamin SK, Willaert A, Parichy DM, Coucke P, Kwon RY
eLife6(2017 09)

Loss of Type I Collagen Telopeptide Lysyl Hydroxylation Causes Musculoskeletal Abnormalities in a Zebrafish Model of Bruck Syndrome.
Gistelinck C, Witten PE, Huysseune A, Symoens S, Malfait F, Larionova D, Simoens P, Dierick M, Van Hoorebeke L, De Paepe A, Kwon RY, Weis M, Eyre DR, Willaert A, Coucke PJ
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research31(11)1930-1942(2016 11)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Excessive activity of cathepsin K is associated with cartilage defects in a zebrafish model of mucolipidosis II.
Petrey AC, Flanagan-Steet H, Johnson S, Fan X, De la Rosa M, Haskins ME, Nairn AV, Moremen KW, Steet R
Disease models & mechanisms5(2)177-90(2012 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following plod2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the plod2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa30802	NM_001007377.2 Latest version!	Danio rerio procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2 (plod2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa31531	NM_001089464.1 Latest version!	Danio rerio procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2 (plod2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

plod2 ( NM_001007377.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001007378.2 Danio rerio procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2 (plod2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa30802
Clone ID Related Accession (Same CDS sequence)	NM_001007377.2
Accession Version	NM_001007377.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1554480000000
Organism	Danio rerio(zebrafish)
Product	procollagen-lysine,2-oxoglutarate 5-dioxygenase 2 isoform 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ020094.1. On Jul 25, 2008 this sequence version replaced NM_001007377.1. ##Evidence-Data-START## Transcript exon combination :: DQ020094.1, BC162509.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505392, SAMEA4476732 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCGGC GTCGGGGTTT TCACGCGTTC GTTTTGATGC TGGTGACTTG TGTTCATTGT 
ACCTTGGGCA TGGAGACAAA TAAGGATATT CCAACAGAAA AACTGCTTGT ACTTACTGTT 
GCAACACAGG AGACGGATGG CTTTCTCCGC TTCATGCAGT CAGCAAACTA CTTCAACTTC 
AATGTGAAGG TGCTTGGGAT GGGAGAAGAG TGGAAGGGAG GTGACGTGGG TCACTCCATT 
GGTGGAGGCC AGAAAGTTCG ACTGCTGAAG GAAGCCATGG AGTCTCTGGA TCAACAAGAG 
GACTTGGTGG TCTTGTTTGT CGATAGTTAT GACCTCATCT TTGCTGGTGG AGCTGAGGAG 
ATTCTCAGAA AGTTCCAGCA GAGCAATCAT AAGGTGGTGT TTGCTGCGGA GGGAATCATC 
TGGCCAGATT CTCAACTGGC AGAGAAGTAT CCGTCTGTAC GCAGTGGGAA ACGCTTTCTC 
AACTCTGGAG GGATTATTGG CTATGCCCCA TACATACAGA AGCTTGTCAG CCAGTGGGAC 
CTTCATGATA ACGACGACGA TCAGCTTTTC TACACGAAGA TCTACGTGGA TCCGATTCAG 
AGGGAAAAGC TTAACATGAC CTTGGACCAC AAGTGTGAGA TTTTCCAAAA TTTGAATGGA 
GCTCTGGATG AAGTTCTCCT GAAGTTTGGC ACGGAGCGAG TACGCGTGAG GAACACCATC 
TACAATAGTC TGCCAGCAGT TATACATGGA AATGTGAACA CCAAGGTATA CTTTAACTAC 
CTGGCCAACT ACATTCCCAA TGCATGGAAC TATGAGCGCG GCTGCACCAT CTGTGACCAA 
GACATGGTGG ATTTGTCTCA GCTTAAAGAG TTTCCACAAG TGACTGTTGG CGTATACATT 
GAGCAGCCGA CTCCATTCCT GCCAGAGTTT CTCGAGCGGC TCCTGAGTCT GGATTACCCC 
AAAGACAAAT TGAATATTTT TATTCATAAC AGCGAGGTTT ACCATGAGAA GCACATACAG 
AAGTTCTGGG AAGAGAACAA GGATGTGTTC GGCAGTTTTA AAGCCGTTGG CCCTGAGGAG 
AACTTAACTC AAGGAGAAGC AAGGAACATG GGCATGGATG TGTGCCGGAG AGATCCTTCT 
TGTGATTATT TCTTCAACAT TGACGCGGAC GTGATGCTCA CGAACCGACA GACTCTGAAA 
CTCCTCATCG AGCAAAACAG AAAAATAATT GCCCCCCTTG TCACCCGTCA CGGAAAACTT 
TGGTCCAACT TCTGGGGAGC TCTGAGTCTG GATGGTTATT ATGCCAGATC TGAGGACTAC 
ATTGACATTG TGCAAGGAAA GCGAGTCGGA GTGTGGAACA TTCCTTTCCT GGCCCACGTC 
TACCTCATAA AGGGTCAGAC TCTGAGAAAT GAGCTTAAAG AGAGGAATGT CTTTGTCTTG 
GAGAAACTGG ACCCTGATAT GGCCATGTGC AGGAATGCCA GAGACTTGGG ACTTTTCATG 
TATCTTACAA ATCGGCATGA GTTTGGAAGG CTTATTTCCA CTGCCAACTA CAACACTTCT 
CATTATAACA ATGATCTCTG GCAAATTTTC GAAAATCCTC TGGACTGGAG AGAGAAATAC 
ATCCATGCCA ATTACACACG AATATTCACT GAAAACCTCT TGGAGCAGCC GTGCCCAGAT 
GTTTTCTGGT TCCCTGTGCT CTCGGAGAAA GCCTGCAATG AGCTGGTGGA GGAGATGGAA 
AACCACGGGA CATGGTCTGG TGGAAAACAC GAGGATAAGC GTATCACCGG AGGCTACGAA 
AGCGTCCCGA CAGATGACAT TCATATGAAA CAGATCAATT ACGATCAGGA ATGGCTGCAC 
TTTATCAGAG AATTTATCTC CCCTGTCACT CTCAAGGTTT TCTCTGGCTA TTACACCAAG 
GGCTATGCGA TTATGAACTT TGTGGTGAAG TACACTCCAG ACAGACAAGC CTATCTTCGA 
CCACATCACG ACTCCTCAAC GTTCACCATT AACATCGCTC TCAACAACAA AGGCTTAGAT 
TTCCTGGGCG GAGGTTGTCG ATTTCACAGA TATAACTGCT CCATAGAATC TCCTAGAAAA 
GGCTGGAGCT TCATGCATCC AGGTCGACTC ACTCACCTTC ACGAAGGACT GCCGGTGACC 
AATGGCACGC GCTACATAGC AGTGTCTTTC GTAGATCCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001007378.2
CDS	195..2396
Translation	MERRRGFHAFVLMLVTCVHCTLGMETNKDIPTEKLLVLTVATQETDGFLRFMQSANYFNFNVKVLGMGEEWKGGDVGHSIGGGQKVRLLKEAMESLDQQEDLVVLFVDSYDLIFAGGAEEILRKFQQSNHKVVFAAEGIIWPDSQLAEKYPSVRSGKRFLNSGGIIGYAPYIQKLVSQWDLHDNDDDQLFYTKIYVDPIQREKLNMTLDHKCEIFQNLNGALDEVLLKFGTERVRVRNTIYNSLPAVIHGNVNTKVYFNYLANYIPNAWNYERGCTICDQDMVDLSQLKEFPQVTVGVYIEQPTPFLPEFLERLLSLDYPKDKLNIFIHNSEVYHEKHIQKFWEENKDVFGSFKAVGPEENLTQGEARNMGMDVCRRDPSCDYFFNIDADVMLTNRQTLKLLIEQNRKIIAPLVTRHGKLWSNFWGALSLDGYYARSEDYIDIVQGKRVGVWNIPFLAHVYLIKGQTLRNELKERNVFVLEKLDPDMAMCRNARDLGLFMYLTNRHEFGRLISTANYNTSHYNNDLWQIFENPLDWREKYIHANYTRIFTENLLEQPCPDVFWFPVLSEKACNELVEEMENHGTWSGGKHEDKRITGGYESVPTDDIHMKQINYDQEWLHFIREFISPVTLKVFSGYYTKGYAIMNFVVKYTPDRQAYLRPHHDSSTFTINIALNNKGLDFLGGGCRFHRYNCSIESPRKGWSFMHPGRLTHLHEGLPVTNGTRYIAVSFVDP

Target ORF information:

RefSeq Version	NM_001007377.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2 (plod2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001007377.2

ORF Insert Sequence:

ATGGAGCGGC GTCGGGGTTT TCACGCGTTC GTTTTGATGC TGGTGACTTG TGTTCATTGT 
ACCTTGGGCA TGGAGACAAA TAAGGATATT CCAACAGAAA AACTGCTTGT ACTTACTGTT 
GCAACACAGG AGACGGATGG CTTTCTCCGC TTCATGCAGT CAGCAAACTA CTTCAACTTC 
AATGTGAAGG TGCTTGGGAT GGGAGAAGAG TGGAAGGGAG GTGACGTGGG TCACTCCATT 
GGTGGAGGCC AGAAAGTTCG ACTGCTGAAG GAAGCCATGG AGTCTCTGGA TCAACAAGAG 
GACTTGGTGG TCTTGTTTGT CGATAGTTAT GACCTCATCT TTGCTGGTGG AGCTGAGGAG 
ATTCTCAGAA AGTTCCAGCA GAGCAATCAT AAGGTGGTGT TTGCTGCGGA GGGAATCATC 
TGGCCAGATT CTCAACTGGC AGAGAAGTAT CCGTCTGTAC GCAGTGGGAA ACGCTTTCTC 
AACTCTGGAG GGATTATTGG CTATGCCCCA TACATACAGA AGCTTGTCAG CCAGTGGGAC 
CTTCATGATA ACGACGACGA TCAGCTTTTC TACACGAAGA TCTACGTGGA TCCGATTCAG 
AGGGAAAAGC TTAACATGAC CTTGGACCAC AAGTGTGAGA TTTTCCAAAA TTTGAATGGA 
GCTCTGGATG AAGTTCTCCT GAAGTTTGGC ACGGAGCGAG TACGCGTGAG GAACACCATC 
TACAATAGTC TGCCAGCAGT TATACATGGA AATGTGAACA CCAAGGTATA CTTTAACTAC 
CTGGCCAACT ACATTCCCAA TGCATGGAAC TATGAGCGCG GCTGCACCAT CTGTGACCAA 
GACATGGTGG ATTTGTCTCA GCTTAAAGAG TTTCCACAAG TGACTGTTGG CGTATACATT 
GAGCAGCCGA CTCCATTCCT GCCAGAGTTT CTCGAGCGGC TCCTGAGTCT GGATTACCCC 
AAAGACAAAT TGAATATTTT TATTCATAAC AGCGAGGTTT ACCATGAGAA GCACATACAG 
AAGTTCTGGG AAGAGAACAA GGATGTGTTC GGCAGTTTTA AAGCCGTTGG CCCTGAGGAG 
AACTTAACTC AAGGAGAAGC AAGGAACATG GGCATGGATG TGTGCCGGAG AGATCCTTCT 
TGTGATTATT TCTTCAACAT TGACGCGGAC GTGATGCTCA CGAACCGACA GACTCTGAAA 
CTCCTCATCG AGCAAAACAG AAAAATAATT GCCCCCCTTG TCACCCGTCA CGGAAAACTT 
TGGTCCAACT TCTGGGGAGC TCTGAGTCTG GATGGTTATT ATGCCAGATC TGAGGACTAC 
ATTGACATTG TGCAAGGAAA GCGAGTCGGA GTGTGGAACA TTCCTTTCCT GGCCCACGTC 
TACCTCATAA AGGGTCAGAC TCTGAGAAAT GAGCTTAAAG AGAGGAATGT CTTTGTCTTG 
GAGAAACTGG ACCCTGATAT GGCCATGTGC AGGAATGCCA GAGACTTGGG ACTTTTCATG 
TATCTTACAA ATCGGCATGA GTTTGGAAGG CTTATTTCCA CTGCCAACTA CAACACTTCT 
CATTATAACA ATGATCTCTG GCAAATTTTC GAAAATCCTC TGGACTGGAG AGAGAAATAC 
ATCCATGCCA ATTACACACG AATATTCACT GAAAACCTCT TGGAGCAGCC GTGCCCAGAT 
GTTTTCTGGT TCCCTGTGCT CTCGGAGAAA GCCTGCAATG AGCTGGTGGA GGAGATGGAA 
AACCACGGGA CATGGTCTGG TGGAAAACAC GAGGATAAGC GTATCACCGG AGGCTACGAA 
AGCGTCCCGA CAGATGACAT TCATATGAAA CAGATCAATT ACGATCAGGA ATGGCTGCAC 
TTTATCAGAG AATTTATCTC CCCTGTCACT CTCAAGGTTT TCTCTGGCTA TTACACCAAG 
GGCTATGCGA TTATGAACTT TGTGGTGAAG TACACTCCAG ACAGACAAGC CTATCTTCGA 
CCACATCACG ACTCCTCAAC GTTCACCATT AACATCGCTC TCAACAACAA AGGCTTAGAT 
TTCCTGGGCG GAGGTTGTCG ATTTCACAGA TATAACTGCT CCATAGAATC TCCTAGAAAA 
GGCTGGAGCT TCATGCATCC AGGTCGACTC ACTCACCTTC ACGAAGGACT GCCGGTGACC 
AATGGCACGC GCTACATAGC AGTGTCTTTC GTAGATCCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plod2 ( NM_001089464.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001082933.1 Danio rerio procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2 (plod2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa31531
Clone ID Related Accession (Same CDS sequence)	NM_001089464.1
Accession Version	NM_001089464.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1554480000000
Organism	Danio rerio(zebrafish)
Product	procollagen-lysine,2-oxoglutarate 5-dioxygenase 2 isoform 2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ020095.1. ##Evidence-Data-START## Transcript exon combination :: DQ020095.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505392, SAMEA4476732 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCGGC GTCGGGGTTT TCACGCGTTC GTTTTGATGC TGGTGACTTG TGTTCATTGT 
ACCTTGGGCA TGGAGACAAA TAAGGATATT CCAACAGAAA AACTGCTTGT ACTTACTGTT 
GCAACACAGG AGACGGATGG CTTTCTCCGC TTCATGCAGT CAGCAAACTA CTTCAACTTC 
AATGTGAAGG TGCTTGGGAT GGGAGAAGAG TGGAAGGGAG GTGACGTGGG TCACTCCATT 
GGTGGAGGGC AGAAAGTTCG ACTGCTGAAG GAAGCCATGG AGTCTCTGGA TCAACAAGAG 
GACTTGGTGG TCTTGTTTGT CGATAGTTAT GACCTCATCT TTGCTGGTGG AGCTGAGGAG 
ATTCTCAGAA AGTTCCAGCA GAGCAATCAT AAGGTGGTGT TTGCTGCGGA GGGAATCATC 
TGGCCAGATT CTCAACTGGC AGAGAAGTAT CCGTCTGTAC GCAGTGGGAA ACGCTTTCTC 
AACTCTGGAG GGATTATTGG CTATGCCCCA TACATACAGA AGCTTGTCAG CCAGTGGGAC 
CTTCATGATA ACGACGACGA TCAGCTTTTC TACACGAAGA TCTACGTGGA TCCGATTCAG 
AGGGAAAAGC TTAACATGAC CTTGGACCAC AAGTGTGAGA TTTTCCAAAA TTTGAATGGA 
GCTCTGGATG AAGTTCTCCT GAAGTTTGGC ACGGAGCGAG TACGCGTGAG GAACACCATC 
TACAATAGTC TGCCAGCAGT TATACATGGA AATGTGAACA CCAAGGTATA CTTTAACTAC 
CTGGCCAACT ACATTCCCAA TGCATGGAAC TATGAGCGCG GCTGCACCAT CTGTGACCAA 
GACATGGTGG ATTTGTCTCA GCTTAAAGAG TTTCCACAAG TGACTGTTGG CGTATACATT 
GAGCAGCCGA CTCCATTCCT GCCAGAGTTT CTCGAGCGGC TCCTGAGTCT GGATTACCCC 
AAAGACAAAT TGAATATTTT TATTCATAAC AGCGAGGTTT ACCATGAGAA GCACATACAG 
AAGTTCTGGG AAGAGAACAA GGATGTGTTC GGCAGTTTTA AAGCCGTTGG CCCTGAGGAG 
AACTTAACTC AAGGAGAAGC AAGGAACATG GGCATGGATG TGTGCCGGAG AGATCCTTCT 
TGTGATTATT TCTTCAACAT TGACGCGGAC GTGATGCTCA CGAACCGACA GACTCTGAAA 
CTCCTCATCG AGCAAAACAG AAAAATAATT GCCCCCCTTG TCACCCGTCA CGGAAAACTT 
TGGTCCAACT TCTGGGGAGC TCTGAGTCTG GATGGTTATT ATGCCAGATC TGAGGACTAC 
ATTGACATTG TGCAAGGAAA GCGAGTCGGA GTGTGGAACA TTCCTTTCCT GGCCCACGTC 
TACCTCATAA AGGGTCAGAC TCTGAGAAAT GAGCTTAAAG AGAGGAATGT CTTTGTCTTG 
GAGAAACTGG ACCCTGATAT GGCCATGTGC AGGAATGCCA GAGACTTGAC AGTACATAGA 
GAAAGAGAAT CTCCTTCTCC GGAATCATTC CATATGCTCA GATCCCCAAA GGGACTTTTC 
ATGTATCTTA CAAATCGGCA TGAGTTTGGA AGGCTTATTT CCACTGCCAA CTACAACACT 
TCTCATTATA ACAATGATCT CTGGCAAATT TTCGAAAATC CTCTGGACTG GAGAGAGAAA 
TACATCCATG CCAATTACAC ACGAATATTC ACTGAAAACC TCTTGGAGCA GCCGTGCCCA 
GATGTTTTCT GGTTCCCTGT GCTCTCGGAG AAAGCCTGCA ATGAGCTGGT GGAGGAGATG 
GAAAACCACG GGACATGGTC TGGTGGAAAA CACGAGGATA AGCGTATCAC CGGAGGCTAC 
GAAAGCGTCC CGACAGATGA CATTCATATG AAACAGATCA ATTACGATCA GGAATGGCTG 
CACTTTATCA GAGAATTTAT CTCCCCTGTC ACTCTCAAGG TTTTCTCTGG CTATTACACC 
AAGGGCTATG CGATTATGAA CTTTGTGGTG AAGTACACTC CAGACAGACA AGCCTATCTT 
CGACCACATC ACGACTCCTC AACGTTCACC ATTAACATCG CTCTCAACAA CAAAGGCTTA 
GATTTCCTGG GCGGAGGTTG TCGATTTCAC AGATATAACT GCTCCATAGA ATCTCCTAGA 
AAAGGCTGGA GCTTCATGCA TCCAGGTCGA CTCACTCACC TTCACGAAGG ACTGCCGGTG 
ACCAATGGCA CGCGCTACAT AGCAGTGTCT TTCGTAGATC CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001082933.1
CDS	195..2459
Translation	MERRRGFHAFVLMLVTCVHCTLGMETNKDIPTEKLLVLTVATQETDGFLRFMQSANYFNFNVKVLGMGEEWKGGDVGHSIGGGQKVRLLKEAMESLDQQEDLVVLFVDSYDLIFAGGAEEILRKFQQSNHKVVFAAEGIIWPDSQLAEKYPSVRSGKRFLNSGGIIGYAPYIQKLVSQWDLHDNDDDQLFYTKIYVDPIQREKLNMTLDHKCEIFQNLNGALDEVLLKFGTERVRVRNTIYNSLPAVIHGNVNTKVYFNYLANYIPNAWNYERGCTICDQDMVDLSQLKEFPQVTVGVYIEQPTPFLPEFLERLLSLDYPKDKLNIFIHNSEVYHEKHIQKFWEENKDVFGSFKAVGPEENLTQGEARNMGMDVCRRDPSCDYFFNIDADVMLTNRQTLKLLIEQNRKIIAPLVTRHGKLWSNFWGALSLDGYYARSEDYIDIVQGKRVGVWNIPFLAHVYLIKGQTLRNELKERNVFVLEKLDPDMAMCRNARDLTVHRERESPSPESFHMLRSPKGLFMYLTNRHEFGRLISTANYNTSHYNNDLWQIFENPLDWREKYIHANYTRIFTENLLEQPCPDVFWFPVLSEKACNELVEEMENHGTWSGGKHEDKRITGGYESVPTDDIHMKQINYDQEWLHFIREFISPVTLKVFSGYYTKGYAIMNFVVKYTPDRQAYLRPHHDSSTFTINIALNNKGLDFLGGGCRFHRYNCSIESPRKGWSFMHPGRLTHLHEGLPVTNGTRYIAVSFVDP

Target ORF information:

RefSeq Version	NM_001089464.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2 (plod2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001089464.1

ORF Insert Sequence:

ATGGAGCGGC GTCGGGGTTT TCACGCGTTC GTTTTGATGC TGGTGACTTG TGTTCATTGT 
ACCTTGGGCA TGGAGACAAA TAAGGATATT CCAACAGAAA AACTGCTTGT ACTTACTGTT 
GCAACACAGG AGACGGATGG CTTTCTCCGC TTCATGCAGT CAGCAAACTA CTTCAACTTC 
AATGTGAAGG TGCTTGGGAT GGGAGAAGAG TGGAAGGGAG GTGACGTGGG TCACTCCATT 
GGTGGAGGGC AGAAAGTTCG ACTGCTGAAG GAAGCCATGG AGTCTCTGGA TCAACAAGAG 
GACTTGGTGG TCTTGTTTGT CGATAGTTAT GACCTCATCT TTGCTGGTGG AGCTGAGGAG 
ATTCTCAGAA AGTTCCAGCA GAGCAATCAT AAGGTGGTGT TTGCTGCGGA GGGAATCATC 
TGGCCAGATT CTCAACTGGC AGAGAAGTAT CCGTCTGTAC GCAGTGGGAA ACGCTTTCTC 
AACTCTGGAG GGATTATTGG CTATGCCCCA TACATACAGA AGCTTGTCAG CCAGTGGGAC 
CTTCATGATA ACGACGACGA TCAGCTTTTC TACACGAAGA TCTACGTGGA TCCGATTCAG 
AGGGAAAAGC TTAACATGAC CTTGGACCAC AAGTGTGAGA TTTTCCAAAA TTTGAATGGA 
GCTCTGGATG AAGTTCTCCT GAAGTTTGGC ACGGAGCGAG TACGCGTGAG GAACACCATC 
TACAATAGTC TGCCAGCAGT TATACATGGA AATGTGAACA CCAAGGTATA CTTTAACTAC 
CTGGCCAACT ACATTCCCAA TGCATGGAAC TATGAGCGCG GCTGCACCAT CTGTGACCAA 
GACATGGTGG ATTTGTCTCA GCTTAAAGAG TTTCCACAAG TGACTGTTGG CGTATACATT 
GAGCAGCCGA CTCCATTCCT GCCAGAGTTT CTCGAGCGGC TCCTGAGTCT GGATTACCCC 
AAAGACAAAT TGAATATTTT TATTCATAAC AGCGAGGTTT ACCATGAGAA GCACATACAG 
AAGTTCTGGG AAGAGAACAA GGATGTGTTC GGCAGTTTTA AAGCCGTTGG CCCTGAGGAG 
AACTTAACTC AAGGAGAAGC AAGGAACATG GGCATGGATG TGTGCCGGAG AGATCCTTCT 
TGTGATTATT TCTTCAACAT TGACGCGGAC GTGATGCTCA CGAACCGACA GACTCTGAAA 
CTCCTCATCG AGCAAAACAG AAAAATAATT GCCCCCCTTG TCACCCGTCA CGGAAAACTT 
TGGTCCAACT TCTGGGGAGC TCTGAGTCTG GATGGTTATT ATGCCAGATC TGAGGACTAC 
ATTGACATTG TGCAAGGAAA GCGAGTCGGA GTGTGGAACA TTCCTTTCCT GGCCCACGTC 
TACCTCATAA AGGGTCAGAC TCTGAGAAAT GAGCTTAAAG AGAGGAATGT CTTTGTCTTG 
GAGAAACTGG ACCCTGATAT GGCCATGTGC AGGAATGCCA GAGACTTGAC AGTACATAGA 
GAAAGAGAAT CTCCTTCTCC GGAATCATTC CATATGCTCA GATCCCCAAA GGGACTTTTC 
ATGTATCTTA CAAATCGGCA TGAGTTTGGA AGGCTTATTT CCACTGCCAA CTACAACACT 
TCTCATTATA ACAATGATCT CTGGCAAATT TTCGAAAATC CTCTGGACTG GAGAGAGAAA 
TACATCCATG CCAATTACAC ACGAATATTC ACTGAAAACC TCTTGGAGCA GCCGTGCCCA 
GATGTTTTCT GGTTCCCTGT GCTCTCGGAG AAAGCCTGCA ATGAGCTGGT GGAGGAGATG 
GAAAACCACG GGACATGGTC TGGTGGAAAA CACGAGGATA AGCGTATCAC CGGAGGCTAC 
GAAAGCGTCC CGACAGATGA CATTCATATG AAACAGATCA ATTACGATCA GGAATGGCTG 
CACTTTATCA GAGAATTTAT CTCCCCTGTC ACTCTCAAGG TTTTCTCTGG CTATTACACC 
AAGGGCTATG CGATTATGAA CTTTGTGGTG AAGTACACTC CAGACAGACA AGCCTATCTT 
CGACCACATC ACGACTCCTC AACGTTCACC ATTAACATCG CTCTCAACAA CAAAGGCTTA 
GATTTCCTGG GCGGAGGTTG TCGATTTCAC AGATATAACT GCTCCATAGA ATCTCCTAGA 
AAAGGCTGGA GCTTCATGCA TCCAGGTCGA CTCACTCACC TTCACGAAGG ACTGCCGGTG 
ACCAATGGCA CGCGCTACAT AGCAGTGTCT TTCGTAGATC CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Zebrafish type I collagen mutants faithfully recapitulate human type I collagenopathies.
Proceedings of the National Academy of Sciences of the United States of America115(34)E8037-E8046(2018 08)
Gistelinck C,Kwon RY,Malfait F,Symoens S,Harris MP,Henke K,Hawkins MB,Fisher S,Sips P,Guillemyn B,Bek JW,Vermassen P,De Saffel H,Witten PE,Weis M,De Paepe A,Eyre DR,Willaert A,Coucke PJ

MicroCT-based phenomics in the zebrafish skeleton reveals virtues of deep phenotyping in a distributed organ system.
eLife6(2017 09)
Hur M,Gistelinck CA,Huber P,Lee J,Thompson MH,Monstad-Rios AT,Watson CJ,McMenamin SK,Willaert A,Parichy DM,Coucke P,Kwon RY

Loss of Type I Collagen Telopeptide Lysyl Hydroxylation Causes Musculoskeletal Abnormalities in a Zebrafish Model of Bruck Syndrome.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research31(11)1930-1942(2016 11)
Gistelinck C,Witten PE,Huysseune A,Symoens S,Malfait F,Larionova D,Simoens P,Dierick M,Van Hoorebeke L,De Paepe A,Kwon RY,Weis M,Eyre DR,Willaert A,Coucke PJ

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Excessive activity of cathepsin K is associated with cartilage defects in a zebrafish model of mucolipidosis II.
Disease models & mechanisms5(2)177-90(2012 Mar)
Petrey AC,Flanagan-Steet H,Johnson S,Fan X,De la Rosa M,Haskins ME,Nairn AV,Moremen KW,Steet R

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plod2 cDNA ORF clone, Danio rerio(zebrafish)

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